text:
Want Hair Like Emma Stone's? Take a Few Tips from Her Hairstylist
Emma Stone's hairstylist, Mara Roszak, dishes on her favorite hair products and more!
-Lucia Peters
Emma Stone definitely isn't stingy when it comes to giving her style and beauty team credit. She's sung the praises of makeup artist Rachel Goodwin; she's called stylist Petra Flannery "the voice of reason" behind her killer red carpet ensembles; but who's responsible for her gorgeous hair?
Meet hairstylist extraordinaire Mara Roszak! Emma makes it no secret that Mara is the reason her hair always looks fabulous, whether it's blonde, red, or somewhere in between. After hearing the Gangster Squad star gush about Mara, Refinery29 tracked the hair guru down and had one heck of a conversation with her. About Emma, Mara says, "[she] has really great hair—she has a natural wave in her hair that can be either easily smoothed out because it's not super-frizzy, or easily enhanced. It's kind of the perfect hair." Not that we're jealous or anything! Mara also notes that Emma "just has that hair that holds a curl or a style, which is really helpful for the red carpet because you never know what it's going to look like when you get there."
Everyone's hair needs a little help every now and again, though—Even Emma Stone's. How does Mara deal with it? With a little bit of help from her friends, of course. Take a few tips from Mara and give your hair the very best when it comes to styling products:
Read SAG Awards Hair How-To: Rose Byrne's Glossy Waves + More
– PHYTO Phytovolume Actif volumizer spray ($28, beauty.com): This is Mara's go-to product to get volume and enhance natural texture. "It's not stick," she says, "so you don't feel it in the hair at all. I'm leery of a lot of volumizing products because unles a product is super-lightweight, you are adding weight—which is contrary to what you want to do." She notes, though, that this particular spray works on everyone.
– TIGI Bed Head Superstar Queen for a Day thickening spray ($16.50, drugstore.com): This one is a multi-purpose product to have in your beauty tricks bag. Says Mara, "You can use it as a volumizer, but it's great for a smooth blowout with volume as well."
– Bumble and bumble surf spray ($28, amazon.com): Want that beachy look? Reach for a surf spray. Mara notes, "I keep trying different surf sprays and I just keep coming back to [this one]." Guess it's a keeper!
-Serums and oils: Mara is a big believer in hair serums, especially for your ends. "It helps in the blowdry process," she says; "your hair gets really smooth and shiny when you use one." There are ways to use serums and oils and ways not to use them, though. For the best results, Mara says, "Whenever you apply anything creamy or oily, always start at the ends and work your way up. Less is more with products like that—you can always add more." Her favorites include Couture Color Pequi oil ($32, amazon.com), KeSARI Indian Oil hair serum ($31.50, amazon.com), and Model Cosmetic Rose Veil hair serum ($45, amazon.com) for damp, towel-dried hair, and Leonor Greyl huile de palme ($50, shop.nordstrom.com) for dry, unwashed hair.
Now go forth and make your hair fabulous, Bettys!
Lucia Peters is BettyConfidential's senior editor.


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Welcome to Liverpool Public Library
1994 Library HistoryIn January Rita ben Simon became the Adult Services librarian who purchased fiction. Katie Reid was hired as a page.
The Optimists Club of Liverpool donated Assistive Listening Devices to the library for use in the Carman Community Room by patrons who require sound amplification.
From February to June the library ran the Homework Connection program in conjunction with the County library system. Volunteer Homework Helpers assisted students in using library resources in order to complete their school research projects. An intergenerational program "Reading is Ageless" took place in March. Senior citizens from Birchwood Center and West Side Manor read stories and poems and did crafts with elementary school students.
In March the Board voted to name the Circulating Software Collection in honor of past employee Jean Armour Polly. A major undertaking of the Board was to review all of the library's policies. Two readings were held on each policy. The time between the two readings was planned to give the staff and the public time to comment. Over two dozen policies were implemented within a few months.
Reference librarians offered a program "Overcoming Library Phobia" in March and April. Small groups of adults were tutored in the use of the library catalog and collections. Other programs included a resume workshop given by Baldwinsville Business teacher, Joseph Bisignano and a presentation by Miriam Monfredo, mystery author from western New York state. All American Tim Green who was a Liverpool High School graduate spoke at another Meet the Author program.
Nolan Lushington was hired as a consultant regarding the library's expansion project . The Board acknowledged and approved the action of Dr. Wells in acquiring the property at 213 Second Street (adjacent to the library) for $212,000.
The April 4 budget vote for $2.6 million passed 268 to 133. Sharon Fulmer was reelected to the Board of Trustees for another five year term.
In June Dawn Stonecipher was hired to work in the Audio Visual Department as a part time page. Melissa Messere and Chris Dahlin were hired as pages for the Children's Room in August. Kerry Higgins joined the paging staff in October.
"Journaling: Writing Your Own News" proved to be a popular workshop. It was led by Linda Loomis on June 28.
In June Dr. Klaiber resigned from the Board. He was awarded a plaque commemorating over twenty years of service as a library trustee. Dr. Thomas J. Wells was reelected as President, Joe Ostuni, Jr. as Vice President, and Patricia Mautino as Secretary of the Board. Daniel Kelly was appointed to the Board in October to fill the vacant position.
On July 14 the Board met with the Department Heads for an all day meeting. The main topic was the proposed expansion. In mid summer several employees visited area libraries to view space utilization firsthand. The public libraries they visited were in Geneva and Clifton Springs.
Rubber Stamping techniques were the subject of workshops for adults in September. An exhibit of Banned Books attracted attention that same month. For the first time the Art Rental Gallery included jewelry as part of its show.
October saw the beginning of the ninth annual Arts Alive concert series. Held on the first Sunday of the month, the series was supported by the Friends of the Library. This year the Art Rental Gallery Open House was held in conjunction with the jazz program.
Local author Barbara Block ( "Chute and Adders") was the guest speaker at the Meet the Author series on October 16th. That same month Fay Ann Golden was honored by the Friends at their annual membership tea for her many years of dedication and service to the library.
Library employees honored and presented with Merit and Performance Awards in November were Renee Romance, Judy Sibio, and Lisa Murphy.
Also in November was the third annual Cornucopia show. Described as "Arts and Crafts with Distinction," the show was a spin off of the popular Art in the Park. Paintings, stained glass and one of a kind jewelry were some of the wares offered.
In December the Board held two special meetings for the purpose of interviewing six architectural firms who had responded to the Request for Proposals sent out by Director Fay Golden.
The library received a Poets and Persons in New York grant to hold poetry workshops in February and March.

subset: commoncrawl


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ASCO Annual Meeting
June 2, 2013
A recent study comparing five or 10 years of tamoxifen (Nolvadex, Soltamox) therapy for early-stage, estrogen receptor (ER)-positive breast cancer showed that continuing tamoxifen for longer than five years further lowers the risk of a breast cancer recurrence (return of the cancer) and death. ER-positive breast cancer uses the hormone estrogen to grow and spread. Tamoxifen is a type of hormonal therapy that blocks the effects of estrogen on tumor growth and has been proven to lower the risk of a breast cancer recurrence and lengthen the lives of women with early-stage breast cancer. Currently, the standard length of tamoxifen therapy is five years, and women start it right after finishing surgery or chemotherapy.
This study included 6,953 women in the United Kingdom who had been taking tamoxifen for five years and then either stopped taking the drug or continued for an additional five years. Researchers found that both recurrences and deaths decreased with each year of continuing tamoxifen, and the women with the lowest risk of recurrence were those who took tamoxifen for more than nine years. Among the women who took tamoxifen for 10 years, about 17% had a recurrence, compared with 19% of those who took the drug for five years. Longer treatment also lowered the risk of dying from breast cancer. Researchers estimate that, compared to taking no tamoxifen, taking tamoxifen for 10 years reduces the deaths from breast cancer by a third in the first 10 years after diagnosis and by half in later years.
What this means for patients
"Five years of tamoxifen is already an excellent treatment, but we thought that longer treatment might be even better because women with ER-positive breast cancer can have recurrences long after treatment is completed. Until now, though, there have been doubts whether continuing tamoxifen beyond five years is worthwhile," said lead study author Richard G. Gray, MA, MSc, Professor of Medical Statistics at the University of Oxford in the United Kingdom. "This study and a related international study confirm that there is definitely a survival benefit from longer tamoxifen treatment, and many doctors will likely recommend continuing tamoxifen for an extra five years."
The side effects of tamoxifen also increased with longer use. These side effects are similar to menopausal symptoms. They include night sweats, hot flashes, and rare but serious side effects, such as a higher risk of uterine cancer, blood clots, and stroke. In this study, the risk of stroke was not higher for women taking tamoxifen for 10 years, but the risk of uterine cancer was higher. However, the researchers estimate that for every extra uterine cancer that occurs as a side effect of long-term tamoxifen, 30 deaths from breast cancer would be prevented.
Questions to Ask Your Doctor
- What stage of breast cancer do I have? What does this mean?
- What is my hormone receptor status?
- What are my treatment options?
- After treatment is over, what are my options to lower my risk of a breast cancer recurrence?
- What are the risks and benefits of each option?
For More Information
What to Know: ASCO's Guideline on Hormonal Therapy for Hormone Receptor-Positive Breast Cancer


text: The UAH College of Arts, Humanities, & Social Sciences Arts, Humanities, and Social Sciences, which include the study of history, literature, philosophy, languages, social sciences, and the fine arts, are the heart of the core curriculum for each student at UAH. They also comprise an important component in the university-wide honors program. Those with substantial liberal arts exposure will have the advantage over people with narrow training whose careers fall victim to technological change. Studies have shown that liberal-arts graduates move up managerial ranks as fast or even faster than those trained in more narrowly specialized fields. The fine arts bring pleasure and perspective to our lives. The music program takes students to the frontier of creativity with an extensive computer laboratory, in addition to, traditional performance opportunities. In today's trans-national and multicultural business world, programs in foreign languages provide the opportunity for students to study and assimilate the languages and cultures of countries they may be residing in or interacting with in their future business, political, scientific, or educational careers. Political science fosters the cultivation of skills in reasoning, synthesis of complex information, decision-making, and oral and written communication, as students are trained to think clearly, critically, and analytically. Philosophy helps students uncover the assumptions that underlie the opinions they hold and the questions they ask and subjects those assumptions to a critical scrutiny. Communication Arts students gain practical, critical, historical, and theoretical perspectives on human communication, preparing them for work, for social life, and for further academic studies. Undergraduate study in the College of Arts, Humanities, & Social Sciences brings together faculty and students in an exciting atmosphere of creative learning. Within the framework of the requirements established by the Department and the College of Arts, Humanities, & Social Sciences, students design, in consultation with a faculty advisor, a bachelor's degree program of study fitted to their particular interests and needs. Please explore further to see what our college has to offer you not only academically, but financially and socially as well.


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Hi all,
I am working on an eBay Store template for one of my clients, as a result of the environment In this instance am coding the template in Quirksmode.
My client has asked me to repoduce the menu found on his eCommerce Website into his eBay store, something of which I have no problem doing accept for IE which is giving me greif.
The html of the menu us thus, where 'NAME' appears directly below 'PRODUCT':

PRODUCT
NAME A

PRODUCT
NAME B

PRODUCT
NAME C

PRODUCT
NAME D

PRODUCT
NAME E

PRODUCT
NAME F

PRODUCT
NAME G

This renders correctly in all Browsers, accept in IE instead of seeing:
I just get
I can post the CSS as well if required, but does anyone have any idea how I might correct this? Any help much appreciated as this is an important job.
Many thanks!!
Well from that code sample there is nothing that would cause IE to display weirdly so something else like CSS is in play so it might be apt to provide some of CSS that effects the above sample, etc.
This is the CSS for that menu taken from my clients site (not my code). I know this problem does not occur in IE in Standards mode, but unfortantly this isnt an option!
.container {width: 500px; margin: 10px auto;}
ul.menutabs {
margin: 0;
padding: 0;
float: left;
list-style: none;
height: 57px;
width: 100%;
margin-top: 20px;
border-bottom:1px solid #e6c54c;
ul.menutabs li {
float: left;
margin: 0;
padding: 0;
padding-top: 12px;
padding-bottom: 10px;
background: white;
overflow: hidden;
position: relative;
ul.menutabs li.tab_right {
float: right;
margin: 0;
padding: 0;
padding-top: 22px;
padding-bottom: 0px;
background: #e6c54c;
overflow: hidden;
position: relative;
ul.menutabs li a {
text-decoration: none;
color: #76c258;
display: block;
font-size: 1.2em;
text-transform: uppercase;
ul.menutabs li a:hover {
background: #292929;
border-bottom: 1px solid #292929;
color: white;
html ul.menutabs li.active, ul.menutabs li.active a, html ul.menutabs li.active a:hover {
background: #292929;
border-bottom: 1px solid #292929;
color: #13B5E7;
All other browsers:
IE (Quirksmode)
Why are you in quirks mode for IE? Put a doctype in there! It makes browsers behave more like IE5
Yes I understand the need for Doctypes in webpages - eBay templates are written in quirksmode because you cant use a DTD in that environment.
If I didnt understand rendering modes I wouldnt be working in Web Development...
In this instance am coding the template in Quirksmode
overflow: hidden; on the LI seems to be the cause of your problem while running IE in quirksmode.
It looks like you can loose the relative positioning and fixed heights as well.
Then probably about 50 or so percent of people don't deserve to beworking then. I'm including people who do improper doctypes too
proper job - thanks!
I seem to forget not everyone sticks to neat standards compliant code


text:
You won't be able to dismiss this by usual means (escape or click button), but you can close it programatically based on user choices or actions.
"I customized this program with a custom color to match my wedding colors and chose an edge trim to make it really unique. They turned out perfect!"
"I was pleasantly surprised at the quality of the paper and printing for this church program! It's perfect and exactly what we wanted. Really sturdy card. Beautiful! Came quickly and without a hitch."
"I am very pleased with these programs. They fit all of the information I needed them to. I used them just to name the wedding party instead of the wedding ceremony because we're having a very basic ceremony and people generally like to know who's standing up there. The paper is very nice, just like the invitations. The only thing I was a little disappointed about was the top of one of our names was cut off just a little bit. It's not a huge deal and I'm sure most people won't even notice but of course I did because it's my wedding. I thought they would catch something like that while editing them. The previews I saw were too small to see it. Other than that I love the product that I got."
"The paper quailty is superb. I didn't expect it to be as heavy and elegant as it was. It's classic in design and I know my guests will be very impressed. The text is easy to read and the color schemes were able to be tweaked for my wedding palette."
"I loved these programs and the staff at wedding paper divas was very helpful during the creation and proofing process! They are simple and elegant, perfect for my tradtional Catholic ceremony. I wanted to print something on the back, but that is not an option with this product. I was able to fit the short poem on the front cover, so it worked out for me."


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FOR the second match in succession Kendal failed to take maximum points despite building a commanding lead as debutant Phil Mooney scored a stoppage time leveller for Cammell Laird.
After a goalless first-half, Maison McGeechan scored twice in quick succession before Freddie Potter's spot-kick reduced the arrears and Mooney's last-gasp equaliser.
Dave Foster tinkered with his side as Kevin Brown came in at centre-half which meant Jack Smith reverted to his more accustomed left-back role.
Ricky Mercer returned in a midfield role with Callum Hoctor on the left flank and McGeechan through the middle – Danny Mitchley was ruled out with a hamstring injury.
The opening chance fell to the home side on three minutes, Lairds keeper Michael Langley tipping over Colin Watson's header from Danny Coid's cross from the left.
In the tenth minute, McGeechan found himself in the clear after sprinting onto Watson's forward ball only for his half-volley to be turned away by Langley.
After Kendal's solid start, Lairds worked their way back into the game with the lively Craig Cairns drawing a save from Danny MacDonald after exploiting space down the right.
McGeechan's pace was causing problems to the visitors' backline, with the 22-year-old's bright play creating a presentable opportunity shortly before the half-hour mark.
The former Bolton Wanderers academy player pestered Lairds' centre-half Liam Hollet, regaining possession for Coid whose left-footed effort was smothered behind by Langley.
Cairns and Joe Evans both had shots which lacked the necessary power to trouble MacDonald in the latter stages of the opening period as the first-half ended goalless.
After a quiet opening to the second period, McGeechan was once again the man who looked the most likely to break the deadlock.
On 53 minutes, Watson found McGeechan after a neat touch by Coid with the frontman shooting wide after skilfully flicking the ball over the covering defender.
Shortly afterwards, Hoctor robbed Hollet on the left and crossed for an unmarked McGeechan whose right-footed volley was palmed over by Langley.
At the other end, Mooney – a recent recruit from Stafford Rangers – saw a header grasped by MacDonald before rifling over following a drilled cross from Jay Foley.
The deadlock was finally broken on 65 minutes as Coid's defence-splitting pass freed McGeechan who coolly slid the ball beyond Langley.
And two minutes later the home side extended their lead as McGeechan doubled his tally for the afternoon and took his total for the season to 12 in all competitions.
The ex-Ambleside United forward latched onto a John Ross pass down the right-hand side of the penalty area before angling a powerful drive across Langley into the top corner.
Kendal lost a three-goal lead in the final quarter of the game at Wakefield seven days previously and with 71 minutes on the clock, Tony Sullivan's side halved the deficit with a contentious penalty.
Cairns was in the clear and bearing down on goal when he was adjudged to have been brought down by Smith, despite the full-back appearing to play the ball.
Having given the penalty the referee chose not to issue a red card despite Smith being the last man as Potter sent MacDonald the wrong way with his spot-kick.
Ironically, Lairds then had two penalty claims waved away which were arguably better shouts than the one given.
By this stage Kendal were offering little in attack as the visitors searched for an equaliser, substitute Jamie Henders squaring for Cairns to side-foot wide with five minutes remaining.
Deep into injury time, Evans floated a free-kick into the box, Macdonald came but failed to collect with Mooney stabbing home following a rather hashed effort by Hollet.
Kendal have lost only once in their last six games but find themselves ten points outside the play-off places in ninth, with all teams above them having games in hand.
Kendal Town: Danny MacDonald, John Ross, Jack Smith, Warren Beattie (c), Will Bell, Kevin Brown, Ricky Mercer, Danny Coid (Rob Wilson 87), Maison McGeechan, Callum Hoctor, Colin Watson (Mike Rushton 82). Subs not used: Ollie Mathieson, Michael Stringfellow
Cammell Laird: Michael Langley, Freddie Potter, Paul Parle, Liam Hollet, Chay Dysart(c), Daniel O'Brian (Paul Carney 90), Jay Foley (Jamie Henders 72), Joe Evans, Craig Cairns, Phil Mooney, Ritchie Foulkes. Subs not used: Paul Lundon, Jamie Speare.
Attendance: 146


text:
Went to Stubbs the other day since it was in walking distance from my hotel. Pretty good, but I like the brisket from my backyard better. Definitely still better than any brisket I've had from restaurants in SC.
The brisket at Stubbs is smoked with Post Oak, according to the waiter with whom I spoke. It was a little heavy for my taste, but it wasn't terrible.
The brisket definitely had a great smoke ring... But it was falling apart and a tad dry... Their sauce was good though and it helped with the dry factor.
Hopefully I'll get to try out Franklins tomorrow. And sorry for the big pix....using my phone this week.


text:
Wright, Astros' bullpen can't corral Rangers
After Keuchel's solid start, reliever gives up go-ahead shot in seventh
HOUSTON -- The last thing Astros veteran relief pitcher Wesley Wright wanted to do was come into a tie game and tiptoe around the strike zone and wind up with a mess on his hands. Wright instead challenged Rangers left fielder Jeff Baker with a first-pitch fastball and wound up paying for it.
Baker cranked a tiebreaking solo home run to the opposite field on the first pitch Wright threw in the seventh inning to send the Rangers to a 4-2 win over the Astros in the series opener between the American League West intrastate rivals Friday night at Minute Maid Park.
"I came in trying to get ahead, and he was able to drive it out to the opposite field, which you have to tip your hat when guys are able to do that," Wright said.
Wright, who was unwittingly at the center of controversy Thursday when he was brought into the game but taken out prematurely before throwing a pitch, suffered his first loss of the season for the Astros, who have dropped 17 of 21 to the Rangers.
Baker had never faced Wright and wound up with his fourth homer of the season in only 36 at-bats. David Murphy socked the Rangers' third homer of the night in the ninth inning, off Travis Blackley.
"It was a good pitch to hit," Baker said. "I don't swing at the first pitch a lot, but it was a good pitch, so I swung and let it go."
Wright replaced left-hander Dallas Keuchel, who made his first start of the season and held the Rangers to two runs and five hits in six solid innings. He walked Elvis Andrus with the bases loaded in the third and gave up a long game-tying homer to Nelson Cruz in the sixth.
"Dallas was tremendous," Astros manager Bo Porter said. "He did a great job attacking the zone, sinking the ball away and was able to pitch to both sides of the plate and get a lot of ground balls and kept us right there for six innings."
Rangers manager Ron Washington lauded the performance of the lefty.
"He always throws good against us," Washington said. "He threw a cut fastball, he sinks it, he's around the plate and he changes speeds. Guys like that give us trouble. He was competing out there and he held down a pretty good offensive team."
Keuchel, who made his Major League debut against the Rangers a year ago, had pitched six games in relief for the Astros this year while making one start at Triple-A Oklahoma City. He's a starter by trade, and his quality outing against the Rangers was a boon to an Astros rotation that's righted the ship after a rough spell.
"I felt like I could have gone longer," said Keuchel, who threw a season-high 83 pitches. "I felt like I was on a pretty good roll with everybody grounding out, except for that mammoth bomb I gave up. I guess he wanted to get me out of there on a positive note. That third inning I walked the bases loaded, that's the run I'd like to have back. I was attacking the zone in that sixth inning, and the only pitch I would like to have back is that bases-loaded walk."
Porter was indeed trying to protect Keuchel when he pulled him after six innings.
"That's the most pitches he's thrown all year," the manager said. "You don't want to put him in a [bad] position. He's pitched too well to go back out for the seventh inning and then you get one guy on base and I'm going to go get him, and now you're asking someone else to save his runs. I'm one of those that really believes in quality, positive starts, and that there is a positive start."
Bringing Wright, a lefty, into the game to face a string of right-handed hitters at the bottom of the batting order might have been a tad unconventional, but Porter's right-handed options were limited to Paul Clemens and closer Jose Veras.
"If you bring Clemens in that spot, he's not going to face [Baker], he's going to face Murphy [a lefty bat]," Porter said. "I felt more comfortable with Wesley facing the bottom of that lineup."
The Astros, who left the bases loaded in the first inning, got a leadoff homer from Trevor Crowe in the second in his first start of the season, which came in left field. In the third, Jose Altuve doubled and wound up scoring on a wild pitch to put the Astros ahead, 2-1.
Crowe's homer off Rangers starter Alexi Ogando was his first since July 23, 2010, a span of 234 at-bats.
"It's good to get your team off to a lead, but I would have liked to have had more consistent at-bats throughout the game," Crowe said.
Brian McTaggart is a reporter for MLB.com and writes an MLBlog, Tag's Lines. Follow @brianmctaggart on Twitter. This story was not subject to the approval of Major League Baseball or its clubs.


text:
4th District Archives
Arizona congressional races too close to call
November 7, 2012 by in Business News | Politics
Arizona's three competitive congressional races were too close to call early Wednesday, including the Tucson-centered seat Rep. Ron Barber won in June to replace his former boss, Gabrielle Giffords. Barber saw his small lead against Republican Martha McSally in the 2nd District disappear just before midnight, with McSally taking a tiny lead. "We knew it… Read More →


text:
Smart social marketers know that good content adds up to good business results. As the leader in social analytics technology, Adobe® Social helps you see and predict what customers like, talk about, and share most. Adobe Social monitors over 100 engagement metrics, filters social listening data by keywords and sentiment, and ties it all back to website activity so you can see which days, times, and types of content spark the most engagement.
Discover what types of posts drive the most likes, retweets, and shares across your social networks so you can optimize your strategies to drive the greatest results.
Use filters to sort posts by platform, data range, type, and content to compare performance and identify trends.
With predictive insights, you can see how well a post will perform before you publish it. Adobe Social also gives you recommendations on ways to improve your posts.
Monitor competitor social pages and posts to gauge your brand performance, learn best practices, and capitalize on opportunities.


text:
ST. JOHNS COUNTY, Fla. - Interstate 95 between County Road 210 and International Golf Parkway was shut down for several hours Thursday morning following multiple serious crashes in the area.
Two people were taken to the hospital by helicopter after two separate serious crashes on that stretch of I-95 in St. Johns County, according to the Florida Highway Patrol.
About 4:50 a.m., FHP troopers responded to the first crash involving a tractor-trailer and three cars on I-95 southbound near International Golf Parkway and Mile Marker 324.
A trooper told Channel 4 at the scene that a semi carrying hazardous materials was hit by a car driven by 23-year-old Jennifer Edwards, of St. Augustine, then another semi struck that car. The driver of the first car got out, only to be hit by yet another car.
No others involved in that crash were injured. Troopers said charges and blood alcohol content results are pending.
"You have to be very cautious after an incident occurs," FHP Sgt. Dylan Bryan said. "If you're able to move the vehicle from the roadway or the lanes of travel, we strongly suggest you do that that way if a secondary collision doesn't occur behind you."
Two left lanes southbound remained closed after 9 a.m. due to the initial crash, as well as one left lane northbound due to debris from the crash.
A couple of hours after the first wreck at about 7:05 a.m., another accident occurred about four miles away. Troopers said the driver of a Ford Explorer, 38-year-old James Harris of Palm Coast, wasn't paying attention, veered to avoid the slow traffic, sideswiped a big rig and rear-ended a car driven by 32-year-old Jolie Hoyt. A helicopter was brought back to the area to take Hoyt to Orange Park Medical Center with life-threatening injuries. Harris suffered serious injuries, and two other drivers involved in the crash had only minor injuries, according to FHP.
Troopers said charges are also pending in that crash.
The crashes resulted in a major traffic back up just north of C.R. 210 on I-95 southbound. Drivers were advised to avoid the area and to take U.S. Highway 1 as an alternate route.
All lanes were clear and reopened to traffic about 11 a.m. Thursday.
Copyright 2013 by News4Jax.com. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


text:
New year, new line-up of food festivals. Up first: the New Times Street Eats Food Truck Festival at Salt River Fields on January 12 & 13, 2013.
But be aware: The Street Eats Food Truck Festival has more than just a few food trucks available for your choosing. At this two-day event, you'll also find a wine lounge, a special VIP tent, several bars, a kids zone, eating contests (hot dog, pie and Twinkie), cooking demonstrations, live music and special guests.
Celebs & demos
Anyone who watches The Food Network and the Travel Channel will recognize these two special guests: Chef Geoffrey Zakarian and Adam Richman.
On Saturday, Chef Zakarian, Iron Chef on The Food Network's hit show "Iron Chef America" and a judge on The Food Network's cooking challenge "Chopped," will perform a one-hour public cooking demonstration from 1-2 p.m.
On Sunday, attendees can check out a one-hour cooking demonstration by Adam Richman, host of "Man vs. Food," "Man vs. Food Nation" and "Adam Richman's Best Sandwich in America" on the Travel Channel. The demonstration will also take place from 1-2 p.m.
The Sub-Zero Wolf Cooking Demo Stage will also include the following demonstrations:
12-1 p.m.: Top of the Rock – Chef Gavino Borquez
12-1 p.m.: The Food Network's Geoffrey Zakarian
2-3 p.m.: Uncle Sal's/The Side Door/The Blind Pig – Chef Jimmy Molinari
3-4 p.m.: Pure Sushi Bar – Chef Eddie Aguilar
4-5 p.m.: Wildhorse Grille at Talking Stick Golf Course – Chef Patrick Karvis
12-1 p.m.: TheHopelessHousewife.com Chef Erika Monroe-Williams & Arizona Diamondbacks Third-base Coach Matt Williams
12-1 p.m.: Travel Channel's Adam Richman
2-3 p.m.: PS Catering Arizona – Chef Chris Peil & Diane Stern
3-4 p.m.: Certified Holistic Health Coach – Chef Michele Rusinkp
Spend a little extra dough
Why merely watch Chef Zakarian and Richman when you spend spend a little one-on-one time with them? Spend the extra cash for admission into the LTB Concepts VIP Tent. Admission includes not only includes light appetizers, $20 in food tickets, six alcoholic drinks and unlimited soda and water, but also a VIP Meet & Greet with Chef Zakarian on Saturday and a VIP Meet & Greet with Richman on Sunday.
Wine time
Want wine? Head to the Stark Raving Un-Wine-D Lounge for eight wine tastings with food pairings.
Here's a rundown of what to expect at the Un-Wine-D Lounge:
- Paired with assorted cheeses and crackers: Chandon Rośe and Moët Ice
- Paired with caprese skewer: Uppercut Sauvignon Blanc and Chalone Chardonnay
- Paired with crustini with tomato relish: A BY Acacia Pinot Noir and Stellina de Notte Chianti
- Paired with beef chimi churi: Terrazas Malbec and Dynamite Cabernet Sauvignon
- Paired with shrimp shooters: A by Acacia Chardonnay and Cloudy Bay Sauvignon Blanc
- Paired with beef empanada: BV Napa Cabernet and Numanthia Termes
- Paired with cheesecake bits: Stellina di Notte Prosecco and Clicquot Demi Sec
- Paired with toasted marshmallow and chocolate bars: Rosenblum Desiree and Newton Merlot Unfiltered
Beer, please
As if it couldn't get any better, the Street Eats Food Truck Festival will even include four bars, including Whiskey River Saloon, Upper Crust Pizza Patio & Wine Bar, Majerle's Sports Grill, Firehouse and a mobile bar from the National Taco Association.
For more information about each bar's featured products and mixed drinks, visit saltriverfields.com.
Food trucks, galore
From frybread, hot dogs and noodles to pizza, kettle corn, burgers and traditional Native American food, nearly every type of food will be present.
For the full list of food trucks, visit saltriverfields.com.
Ticket prices
Sold yet? Here's what you can expect to fork up at the door:
- $10 General Admission Tickets / Children 12 and under free
- $35 Un-Wine-d Lounge
- $60 LTB Concepts VIP Tent
For more information about the Street Eats Food Truck Festival, including the line-up of live entertainment, visit saltriverfields.com.
New Times Street Eats Food Truck Festival
When: January 12-13, 2013 from 11 a.m. to 6 p.m. (each day)
Where: Salt River Fields at Talking Stick
7555 N. Pima Rd., Scottsdale
Tickets: Purchase tickets here


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Panel Discussion: "Cyber Security – Uncharted waters for the UN"
Panel Discussion: "Cyber Security – Uncharted waters for the UN"
Thursday, 6 June 2013, 1.00 - 2.45pm, at the German House
News reports on cyber security have become regular reading for us all. In contrast, cyber issues are rarely discussed within the United Nations. It almost seems as if cyberspace doesn't exist for the UN. These are uncharted waters for the UN despite the fact that maintaining security of cyberspace is emerging as a significant challenge for the international community.
There is growing concern regarding the wide range of potential cyber attacks and their potentially devastating consequences. Rapidly advancing technological capabilities have given rise to mounting risks of escalation and miscalculation.
In the military arena of the past, policies of deterrence, classic non-proliferation strategies and export controls have prevented major destructive escalations. These instruments, however, have been applied to traditional instruments of war and wellestablished scenarios of warfare. Those were based on the use of weapons and arms that were real, not virtual. Although the virtual theater of war and its actors may be invisible to most of us, the impact on the military operations involved may be very real and extend well beyond a purely military dimension.
Securing cyberspace therefore raises a new spectrum of questions for the international community:
· How could conflicts in cyberspace potentially escalate?
· What are likely scenarios?
· How can misinterpretations be avoided?
· What kind of counterstrategies are available?
· Is there clarity about the applicable international rules and norms?
· What should be the role for the UN?
· Should there be one at all?
The Panelists:
· James Lewis, Center for Strategic and International Studies in Washington, D.C.,
· Cherian Samuel, Institute for Defence Studies and Analyses, New Delhi,
· Sandro Gaycken, Freie Universität, Berlin.
The panel will be moderated by Colum Lynch, veteran UN correspondent of the Washington Post and a blogging contributor to Foreign Policy.
The event is organized in collaboration with the EastWest Institute and directed at a broader UN public. It intends to offer a space for dialogue, involving Member States, UN agencies and NGOs.
Space is limited and reservations are required. If you like to attend, please send an e-mail to pol-s5-vn[at]newy.auswaertiges-amt.de.


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National Election
Cameron wins 1922 Committee reform
12:44pm Thursday 20th May 2010
David Cameron's controversial bid to reform a key Tory backbench committee has been passed by his MPs.
Conservative MPs voted by a margin of 168 to 118 to accept a change that will see ministers able to play a full part in the 1922 Committee.
Traditionally, membership has been limited to backbenchers when the party is in government.
The result, confirmed by a party spokesman, should give the Prime Minister more control over a body that has historically been a thorn in the side for Tory leaders.
A number of MPs have expressed public concerns over the move, which was sprung on the party at a meeting in the Commons on Wednesday night.
Prominent figures including Douglas Carswell, Bill Cash, Christopher Chope and Peter Bone have suggested the reform will reduce accountability and effectively abolish the 1922.
The result of the vote indicates that many more privately agreed with the sentiments - although not enough to prevent Mr Cameron getting his way.
The crucial change means that the "payroll vote" of ministers and Government Whips will be able to take part in the election of the 1922's new chairman on May 26 - the day after the Queen's Speech.
Veteran Richard Ottaway, the current vice-chairman, is fighting Altrincham and Sale West MP Graham Brady, who has been critical of the leadership, for the coveted post.
If elected, many expect Mr Brady to become a focal point for growing backbench disquiet over the coalition deal Mr Cameron has struck with the Liberal Democrats.He has already indicated he would have preferred the Prime Minister to form a minority Tory administration.


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Ilkley Civic Society will discuss the forthcoming Bradford Local Plan at its meeting this month, just days before a public meeting called by Ilkley Parish Council on the issue.
The Civic Society will meet at Church House, Church Street, on Thursday, January 23, inviting members to have their say on the plan, which will influence housing and business building developments in the Bradford District until 2029. The meeting will begin at 7.30pm.
Ilkley Parish Council has also organised a public meeting, at the Clarke Foley Centre on Wednesday, January 29, to discuss the proposed Core Strategy of the Local Plan.


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Advance Tabco BS-308 Wood Top Baker's Table with Stainless Steel Undershelf - 30" x 96"
Other Available Sizes:
- Laminated, 1 3/4" hard maple top with 4" high sides
- Stainless steel undershelf
White Glove Delivery - Available -What is White Glove service?
Standard common carrier delivery options cover the cost of getting an item to your shipping address but our White Glove delivery service does that and more!
The White Glove agent will call to schedule a delivery appointment within a four hour window, which gives you the flexibility to work around your busy schedule and to prepare the space where your item will be placed. Upon delivery, trained professionals will unload your item from the truck, bring it into the room of choice, unpack the item and remove all packaging debris from the premises.
Please note that White Glove delivery will typically add 5 - 7 business days to your order's transit, and only includes 30 minutes of on-site time. If on-site time beyond 30 minutes is required, you may be responsible for additional fees. Additionally, this service does not include set-up of the items being delivered.
You May Also Need
"You May Also Need" items are guaranteed to work with the item you are viewing, such as lids that will fit a cup or casters that will fit a piece of equipment. If an item is not listed in this section, we do not guarantee that it will work with the product you are viewing and do not recommend that you purchase them for use together.
5" Swivel Stem Casters for Work Tables and Equipment Stands - 6/Set
$119.99 /Set
Advance Tabco US-30-96 Adjustable Work Table Undershelf for 30" x 96" Table - 18 Gauge Stainless Steel
$319.05 /Each
Emmet's Elixir Wood Conditioner / Cutting Board Oil 16 oz. Squeeze Bottle Michigan Maple Block Co.
$11.99 /Each
|96 Inches
|30 Inches
|35 1/2 Inches
|Backsplash Height
|4 Inches
|Leg Construction
|Stainless Steel
|Made in America
|NSF Listed
|Number of Legs
|Table Style
|Tabletop Material
|Undershelf Construction
|Stainless Steel
Customer questions about this product
- What is the best way to clean and care for my wood top table?
- All wood products should be hand washed. If your wood top begins to show signs of wear, its finish can be restored with a food safe wood conditioner. First, clean and dry the wooden top. Then, apply the wood conditioner with a soft cloth or paper towel. Once the conditioner has soaked into the top of the table, buff off any excess with a clean cloth. Having excess conditioner on a wood top will make it feel sticky or oily.
- Will using casters on my Advance Tabco work table or equipment stand increase the working height?
- As an added service, Advance Tabco will automatically send shorter table legs when you purchase one of their work tables or stands along with a set of Advance Tabco casters. The shorter legs will guarantee that your table's height will remain the same as is listed on the table's product page, ensuring a comfortable working height for your needs.
This Advance Tabco BS-308 baker's table features a laminated, 1 3/4" thick hard maple wood top with 4" high coved corner risers to keep flour and other ingredients on the table and not on the floor when rolling dough or preparing baked goods.
Thanks to its durable 18 gauge stainless steel undershelf and six 1 5/8" diameter stainless steel legs, with 1 1/2" adjustable feet for precise leveling, the Advance Tabco BS-308 wood top baker's table makes an ideal work station for the busiest commercial bakeries.
Overall Dimensions:
Left to Right: 96"
Front to Back: 30"
Height (excluding corner risers): 35 1/2"
This Item Ships via Common Carrier. For more information and tips to help your delivery go smoothly, click here.
This item is made-to-order and cannot be canceled or returned once your order is placed. Please contact Customer Solutions prior to placing your order if you have any questions or concerns.
Because this item is not stocked in our warehouse, processing, transit times and stock availability will vary. If you need your items by a certain date,
please contact us prior to placing your order. Expedited shipping availability may vary. We cannot guarantee that this item can be cancelled off of the order or returned once it is placed.
Best in Class
This product was given a Best in Class Award in Foodservice as chosen by foodservice operators, dealers, and consultants.
NSF Listed
This item meets the standards imposed by NSF International, which focuses on public safety, health, and the environment.
Made in America
This item was made in the United States of America.
A PDF viewer is required to view this product's information. Download Adobe Acrobat software
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I am lifestyle
You will love to carry your water or any other beverage in these ultra-durable, eco-friendly, stainless steel bottles with screw on cap.
Lightweight, reusable, BPA-free and designed to last a lifetime. Comes in 7 designs: Love Miami, Peace, Love, Happiness, Life, Sunshine and Karma.
Cap size: 1.75"
Bottom size: 2.0"
Capacity: 25oz
Cannot be put in the dishwasher.
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A BUSINESSMAN from Ilkley has completed his seventh major annual yachting race after being inspired by his wife's heroic fight against breast cancer.
Christopher Barker, along with wife, Zoe, and their two children, Annabelle and Serena, recently took part in the 2014 JP Morgan Asset Management Round the Island Race, sailing around the Isle of Wight.
The Barkers are no strangers to the seas, and have been sailing as a family for several years. Mr Barker decided to raise money for good causes by competing in the race.
The race is 50 nautical miles - or 93 kilometres - long, and more than a thousand boats regularly take part.
The first time Mr Barker competed was back in 2007 and he has made sure that he has returned every year since.
This year's race saw thousands of competitors competing against big names such as four-time Olympic champion Sir Ben Ainslie, and Dame Ellen MacArthur.
But for Mr Barker, 46, and his family, it was all about being able to spend time together as a family and raise money for good causes.
"Seven or eight years ago my wife Zoe had a diagnosis of breast cancer and she went through a lot," said Mr Barker.
"She's been truly heroic since so that was an emotive year and we did a lot of different things and of those mad things that we did was the Round the Island Race and we said we'll raise whatever we can.
"So we did quite well as a charity event and that was our first one in 2007 and we've returned every year since and Zoe came a few years later when she was in good health.
"So we've been doing it as a family for years now and the young girls are old enough to do it with us.
"The excitement is incredible and we make a big family weekend of it and it's great to have come back this year."
It is the second time the whole family has had the chance to compete in the event and Mr Barker says they plan to take part in more in future.
"To have so many boats on the start line is amazing because it's 20 times you'd expect usually.," Mr Barker added.
"That creates some really great close-quarter situations and teamwork situations and it is so variable. It's an event we will continue to do for years to come."
The J.P. Morgan Asset Management Round the Island Race is one of the largest participation sporting events in the UK and the largest yacht race of its kind in the world. Organised by the Island Sailing Club in Cowes, it is dubbed 'Britain's favourite yacht race', regularly attracting around 1,500 boats and 16,000 competitors.


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Your gift to help a hardworking entrepreneur will double in impact thanks to a generous World Vision partner.
James is from Kenya. He has 3 children. He needs a loan of $500 to purchase fertlizer and seeds.
James is from Kenya. He has 3 children. He needs a loan of $500 to purchase fertlizer and seeds.
James Mwangi is a father of three children, he lives with his family in Ngondi, a rural part of Kenya. James is a hard working farmer, growing and selling his crops to traders and markets in his local community, to help him earn a living for his family.
With a loan from World Vision, James will be able to grow more crops, which will allow him to expand his business and generate more income. This is especially significant when his country is battling a drought. With the additional income, James will be able to provide his family with better living conditions and provide for their daily needs.
The Agriculture business sector covers all farming and livestock activities. Some entrepreneurs request loans to help in securing supplies and equipment. Others want to buy more animals to breed or purchase feed and medicines. The majority of our loan clients live in rural communities where agriculture is already understood as a business model. It is for this reason that approximately 50% of our loans are in the agricultural sector.
There is no community update available at this time.
While James Mwangi initially invested his business loan by purchasing fertilizer and seeds to improve his crops, he has struggled to repay his loan. Although we continued to work with James to help him reestablish his business, we regret to report he has now defaulted on his loan.
James prays he will someday be able to restart his business so he can better provide for his family. James would like to sincerely thank all the donors who partnered with him. Please pray for James and his family during this difficult time.
Despite the fact that World Vision boasts a repayment rate greater than 98%, there will always be some loans that do not get fully repaid. While World Vision does everything it can to ensure that this does not happen, it is an unfortunate reality that must be recognized when providing loans to less fortunate populations.
Thank you for your understanding, and your support of World Vision Micro. If you have any questions or concerns, please feel free to call our Customer Care line at 1-888-511-6444.


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Home » Western Saddles & Tack » Western Saddles » Nash Saddlery Pace Cutter Saddle
Nash Saddlery Pace Cutter Saddle
Code: NS4300
Only $859.00
Quantity in Basket: None
American-Made Quality!More than 50 years of saddle making experience - American-made quality, materials, construction, and tooling. Nash Saddlery has been a leader of private label saddlery for half a century and is now offering their line direct to the public.
A great barrel saddle ready for the competition ring! Hand stamped in barb wire and basketweave trimmed with silver conchos. The roughout jockey and fender make for a nice contrast to the beauty of this golden finished saddle.
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Most homeowners don't get around to cleaning their air ducts until the accumulated dust causes the criminals hiding inside them to sneeze all night long. Breathe easy with this Groupon.
Choose from Two Options
- $59 for the cleaning of up to 10 air-duct vents (up to a $250 value)
- $119 for the cleaning of unlimited air ducts (up to a $500 value)
Pro Steamers
Nail polish. Red wine. Paint. Coffee. Ink. Even the most stubborn stains are no match for the technicians at Pro Steamers, who use hot wisps of steam to clean carpets in a way that's as gentle as it is environmentally friendly. They clean carpets and upholstery for both residential and commercial clients, locking in newfound freshness with a layer of Scotchgard, which repels and blocks future stains without embarrassing carpets by making them wear bibs. To further spruce up spaces, they also clean air ducts, scour grout and tiles, and restore areas that have water or fire damage.


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A law firm with offices in Ilkley has committed to raising £2,000 for the Airedale Hospital Emergency Department Appeal to help fund a number of new distraction walls for children.
LCF Law has kick-started its fundraising with a donation from Partner and Head of Personal Law, Neil Shaw.
Neil was sponsored to compete in the Jane Tomlinson 10k race dressed as Elvis Presley.
He said: "I'm not a runner so I knew that friends, family and colleagues would pay good money to see me take part in the race. I completed the race in under an hour which I was very pleased with considering I stopped quite a few times to sign autographs!"
Meg Crossley, consultant in emergency medicine and clinical lead for A&E at Airedale NHS Foundation Trust said: "I am absolutely delighted that LCF Law is supporting Airedale's new Emergency Department by fundraising towards distraction walls for the children's rooms.
"Children can find coming into hospital, particularly in an emergency situation, quite frightening.
"These floor-to-ceiling distraction walls play a vital role in helping parents distract or entertain a child if they are becoming agitated or distressed.
"The child can then be treated safely and quickly by the medical staff whilst remaining calm and concentrating on the wall. The walls can be wiped clean and are extremely durable as well as being an ideal alternative to plastic toys or books which can harbour germs and can be difficult for staff to hold whilst trying to treat the patient."
LCF managing partner Simon Stell added: "Many of us live locally and understand what a vital role the hospital plays in the lives of so many children so we can really appreciate what a difference the distraction wall will make.
"We have a number of fund-raising events planned and we will make a company donation."


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Water and Light
Your trusted source of water and electricity for over 100 years.
Browse Como.gov Skip top Navigation. Translate this page
Transmission lines in the southern part of Columbia are being built to reliably deliver electricity to this area and meet federal standards. This project involves adding transmission lines to connect the new Mill creek substation on Peach Tree Drive with some of the existing substations. After a special, second public hearing on the route for the transmission lines, the Columbia City Council decided On January 19, 2016 to not build transmission lines according to the Option A route. This project is on hold until further direction is given by the City Council.
QUESTIONS? Call the Electric Transmission Project information line 844-244-7870 (toll free)
Sign up for e-mail updates - select the Southern Columbia Electric Transmission Line project .
2017 Transmission Project Timeline Chart
City Council work session presentation on May 16, 2016
Council information regarding the transmission project on January 19, 2016
Council information regarding the transmission project on November 16, 2015
Download the presentation in a pdf file: July 15, 2013 City Council meeting
Public feedback on electric transmission line project route selection
With the process of this project, it is difficult to easily move back & forth in this process without costing additional time & money
Option A was selected by the City Council as the route for the new transmission lines in 2013 and then the City Council decided against Option A in January 2016. The intent of the Option A alignment study is to connect 161 kilovolt circuits to the Mill Creek substation from the Grindstone, Perche Creek, and McBaine substations. The route will run along Providence, Grindstone/Nifong/Vawter School Road and Scott Boulevard.
Columbia Water & Light staff members support Option A for the following reasons:
memo about Option A that went to Council on April 18, 2011
Option A Selection Matrix Results from 2011
Videos of the proposed pole structures and locations along the Option A Routes
The intent of the alternatives in the Option B alignment study is to connect the Mill Creek substation with 69 kilovolt lines along the existing 69 kilovolt line route from the Grindstone substation to the Hinkson Creek substation. Option B will place the Mill Creek substation in series with the 69 kilovolt circuit from Grindstone to Hinkson Creek substations. In addition, Option B connects the McBaine substation to the Perche Creek substation via a 161 kilovolt line routed along the southwestern periphery of the city limits as Council suggested. Public feedback was used for the evaluation matrix to determine the preferred routes for Option B.
After the publicly preferred route for Option B was identified, Columbia Water & Light staff looked into the possibility of moving a section of the line to city owned property near the Waste Water Treatment Plant, the city's wetland cells and the Water Treatment Plant. These properties also contain a section of the MKT trail. Option B-2 was presented to the City Council in August 2012 and at a public meeting in November.
Option B map - publicly preferred route
Option B-2 map - publicly preferred route
Report to the City Council on 8-20-12: Mill Creek Substation Transmission Study - Option B
Option B Selection Matrix Results & Preferred Routes report from 2012
Presentation to the City Council on August 20, 2012
Determining pole placement along Option A has been stopped due to the City Council reversing its decision on building lines along the Option A route. Proposed pole placement along the route has been determined and comments regarding these locations are being collected. There are a number of factors taken into consideration before a decision can be made, including:
Electric and Magnetic Fields (EMF) are generated by the flow of electrons. Electric fields are established between points of different voltages. Magnetic fields are generated by electrons flowing in a conductor. EMF are generated by electric lines as well as many common household items. EMFs decrease in strength with distance from the source.
According to the Environmental Protection Agency, "Much of the research about power lines and potential health effects is inconclusive. Despite more than two decades of research to determine whether elevated EMF exposure, principally to magnetic fields, is related to an increased risk of childhood leukemia, there is still no definitive answer. The general scientific consensus is that, thus far, the evidence available is weak and is not sufficient to establish a definitive cause-effect relationship.
Please see these reports and web sites for more information about EMF and any possible health issues
U.S. National Cancer Institute on EMF
American Cancer Society on EMF
Comparison of Bio-Initiative on EMF Claims
Environmental Protection Agency - (1 page overview with links)
Electric Power Research Institute – general overview and list of resources on EMF (5 pages)
Electric Power Research Institute - answers to frequently asked questions about EMF (2 pages)
Western Area Power Association – provides a basic overview of EMF and levels of EMF found in power lines (16 pages)
To handle electric load growth in southern Columbia and for reliability purposes. There is a growing need for more electricity on the southern side of Columbia. Substations in the area are approaching their maximum capacity both electrically and physically. This makes them vulnerable to being overloaded which could cause power outages and damage to other equipment in the system.
Transmission systems need to be built so one outage will not cause overloading of another transmission line. If there are two outages, the system must be built so there are not cascading outages. A new substation and transmission lines in Columbia will reduce the loading on the existing substations, ensure greater electric reliability and will improve regional stability for the power grid.
Electricity is generated at a power station or power plant from fossil fuels or renewable resources. Approximately 90% of Columbia's energy comes from sources outside the city. Once the power is generated, it has to be moved to where it is going to be used. Large amounts of power are transferred with electric transmission lines. An interconnected network of transmission lines are commonly referred to as the power grid.
Transmission lines feed into substations where transformers step down the power to lower voltages. From there, power is delivered to individual electric customers by distribution lines. New distribution lines in Columbia must be placed underground according to city ordinances. In the older sections of town, distribution lines are mounted on wooden poles.
Electrical power delivery systems are built with an interconnected network of transmission and distribution lines. That way if there is a problem with one section of an electric line, operators can switch the flow and provide electricity to customers with another line. An interconnected electric grid helps the reliability of the system and can greatly reduce the length of an outage
The Columbia City Council decided to build the new transmission lines overhead. Due to the high voltage of the electricity the lines carry, it is much more involved to bury them than it is distribution lines. Transmission lines are not frequently buried in the United States due to the cost. Placing transmission lines underground in Columbia is estimated to cost $7.6 million per mile versus $1.1 million per mile for overhead construction. The estimated rate impact per customer to build the lines underground would be $8.26 per month for 20 years versus $1.18 for overhead. (NOTE: This information has not been updated since 2013. Since that time, the Columbia City Council has not requested any additional information on the costs of burying transmission lines.
Download a historical time line of this project.
Sega, Inc. was hired by the city to evaluate different transmission line routes to start the public discussion. The consultants divided the project into three different sections and proposed three to four different routes for each section. The initial routes were chosen using these criteria:
After the initial routes were selected, they were presented to the public and the City Council. Written public comment was tabulated to formulate a selection matrix for each route. Columbia Water & Light collected feedback during late 2012/early 2013 to determine the public's most favorable route and the preference for building overhead or underground electric transmission lines.


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Located in the district Aigen in Salzburg in a very quiet neighbourhood, one reaches the city center with the car in around 15 minutes. The property is situated in a neighbourhood of stately mansions near the border of a forest. Stores for the daily need lie within walking distance.
Floor heating in all residential quarters
Floors: wooden parquet and italian marble
Heated towel rail
Electric roller blinds with central control
Synchronised loudspeakers in all residential quarters
Pool with counter current system and humidity removal
Elevator from the basement to the top floor


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Dining out? Check your phone first (if you aren't doing that already); here are some dining apps for you to consider.
With today's plugged-in landscape and social media savvy society, websites, blogs and smartphone applications are quickly becoming the go-to source for daily tasks like hunting down recipes, shopping for restaurant deals and connecting with friends. From check-ins to secret menus and Twitter notifications about the next happy hour, social media is the inroad to "what's good" at bars and restaurants.
At Sapporo, social media has allowed us to give diners a backstage pass to our food and how we make it as well as provide a tailored personal experience. Hungry for a certain dish? Don't worry; Sapporo has you covered. By liking Sapporo on Facebook, users can ask questions and checkout the new and most popular menu items and what people are saying. Through Twitter, users can follow and find out about upcoming events and exclusive promotions.
Whether you're looking for a fine-tuned menu, a trendy, foodie destination (like Sapporo) or just wanting a burger, below are some social media app suggestions that will have you eating, drinking and being social in no time.
Digital dining promotions
Remember the days of cutting coupons? Cut no more; with today's social media landscape, getting the best deals on restaurants is literally at the click of a button. With daily deal sites like Groupon, Living Social and Doozy of a Deal, diners can pre-purchase coupons and print them off to use them at their leisure. But that's not all, more and more restaurants are utilizing their own social networking pages like Foursquare and Facebook to offer exclusive deals and promotions to help drive traffic through the door and create customer loyalty.
There are dining apps for that
For those who may be hungry but too lazy to make a reservation or order food, there's an app for that. Today's social landscape makes it easy for diners to get rid of their hunger pains by the push of a button. At Sapporo, an app called Open Table allows diners to make reservations through Facebook. Other restaurants have also created their own mobile applications that allow customers to place food orders from the convenience of their smartphones and even pay for their purchases.
Table tweets
Dining with social media isn't just about getting the best deals; many restaurant guests enjoy the connection social media allows them to have with the restaurant and its chef. Through Twitter, many restaurants, chefs or owners will actively engage in conversation with the customer base by posting events, recipes, up-to-date menus and photos. Some even take customer interaction one step further by coming out to greet those patrons tweeting from their table.
Mind your mobile manners
"No phone at the dinner table" is a phrase everyone has heard at one time or another. Since mobile technology has improved and diners are more social media savvy, it has become socially acceptable to multitask during meals. Today's guests are eager to check-in, snap photos or tweet about where they are and what they're doing. Not sure what to order? Foodspotting and Forkly, apps that can be downloaded to a mobile device, allows diners to snap photos and share dishes so customers make better-informed purchasing decisions.
Dine and dish
After a dining experience, there is nothing more guests like to do than dish about what they ate and how amazing, or not amazing, it was. Several social networking sites have created a space where patrons can share their tips, reviews and rate restaurants. Urbanspoon is an app that allows customers to view ratings for the specific type of food or restaurant they are looking for. Yelp is another app that allows users to search for the restaurants closest to them and read reviews before choosing a specific restaurant.
In the past, dining out may have involved navigating an unfamiliar neighborhood aimlessly searching for a restaurant, unsure of what to expect on the menu ― but not anymore. Look no further than your smartphone, where restaurants like Sapporo are engaging in the social space and guests can stay in the loop and experience a new type of dining experience.


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When you come to Grove City College, you become a part of a dynamic and supportive community of lifelong learners.
The student newspaper, The Collegian, is owned and
published by Grove City College. The paper is published weekly, free of
charge, with the purpose of reporting happenings on campus and beyond.
If you live nearby, commuting is another way to experience Grove City College.
Students are encouraged to get involved in these groups to learn from one another, make a positive impact on the world and to embark on their spiritual journeys together.
All students admitted to Grove City College enjoy full access to its programs and services.


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Right - not sure how this site works - but ive managed to upload some pics and hope someone can help explain whats been captured...
Last Sunday there was an electrical storm here in sydney, I went outside to film some of the lightning bolts with the aim of getting (eventually) some stills with lightning in them. At no point did I think the lightning was 'close' and yet yesterday when i finally downloaded quicktime in order to view the pics - one of the bolts even APPEARS on my side of the railing - now this doesnt seem feasible to me... so am hoping someone can explain why it APPEARS that way on the photo.
(Its not been 'doctored' and its not fluff on the lens or anything - it appears in one frame - gone in the next).
Here's the pics anyway - hope someone can shed some light on 'em!
The balcony one...
File:Http://chdk.wikia.com/wiki/Image:Too close!!.JP
Bolt between myself and neighbours....
File:Http://chdk.wikia.com/wiki/Image:Des' house.JPG
Two frames that show the lightning going UPWARDS from the ground (frame one) to the sky (frame 2)
File:Http://chdk.wikia.com/wiki/Image:Goin up.JPG
Thanks in advance!


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THE 70th Anniversary of D Day was marked in Guiseley with the placing of a Normandy Veterans Associations wreath at the town's memorial garden.
The wreath was placed by Elsie Turner fromThe Royal British Legion Guiseley branch. Also attending was the area's MP Stuart Andrew, ward councillors, and representatives from local groups who had been to a WW1 projects meeting for Aireborough.
This week Mrs Turner thanked everyone in Guiseley, Rawdon and Yeadon, who had helped to raise £31,433 for the 2013 Poppy Appeal.
She said: "Volunteer collectors stood in Morrisons Guiseley and Yeadon every day for two weeks also two Saturdays at Asda store in Guiseley Retail park.
"The Army Cadet Unit (Yeadon). Collected at Guiseley Football Club and Morrisons. 2168 Air Training Corps (Yeadon) collected at Morrisons,and the squadron band also took part in the Remembrance Day parade. Schools, shops, public houses, and business premises had collecting boxes for two weeks,
"Without all these helpers working as a team we could not achieve this wonderful amount. Your help and support are very much appreciated."
The Royal British Legion will hold a wreath laying service in memory of the centenary anniversary of the beginning of the First World War, at Guiseley memorial garden,Town Gate,on Monday August 4 at 11am, and will also place crosses on war graves in St.Oswalds churchyard. Guiseley brass band will be playing from 10.30 am.


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Students at St. Olaf have the opportunity to study a unique subject — Norwegian. St. Olaf is one of a few U.S colleges and universities where students can use Norwegian to complete their foreign language requirement — and beyond the requirement can study the language, literature, culture,and history of Norway in depth. In addition to graduating with a major in Norwegian, St. Olaf students have many opportunities to study and travel in Norway.
The study of Norwegian opens the door to another culture and another way of viewing the world and develops skills in communication, research, analytical thinking, and writing that are essential in a wide range of careers. Former students report time and again that a knowledge of Norwegian is a rare and appealing skill that gives them an edge over other graduates.
Some of the world's best literature awaits discovery by students of Norwegian: works by authors like Ibsen, Hamsun, and Undset, as well as by writers who have not been translated into English. Norwegian also enables many students to explore their cultural heritage, and encourages contact with relatives and friends in Norway.


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- Read the latest issue of the ProbesOnline E-Newsletter.
In This Issue
Ultrasensitive Reporter Gene Assays — NovaBright™ Reporter Gene Assays
Click-iT® Biology Just Got Brighter — New Alexa Fluor® 555 Click-iT® EdU Cell Proliferation Assays and Detection Reagents
Live-Cell Imaging of Cell Cycle and Division — Premo™ FUCCI Cell Cycle Sensor
Accelerate MAPK Signal Transduction Research — New Validated Primary Antibodies
See all of this month's New Products for Cell & Tissue Analysis
- Buzzworthy — High-efficiency Transient Transduction of Human Embryonic Stem Cell–derived Neurons
- Online Technical Webinars — Fluorescence Imaging & New Product Overview
- The View — Live-Cell Imaging of Cell Cycle and Division
- Proven Performers — Epitope Tag Antibodies
- On the Web — Environmental Sustainability Programs & IDC Latex Microspheres
- In the Field — Post-sorting Analysis Enabled by Low Toxicity of Cell Cycle Stain
Check out the latest issue of BioProbes
High-Content Analysis of Cytotoxicity
Subscribe to BioProbes
what it is
Reporter gene assays are invaluable for the study of gene expression. NovaBright™ Reporter Gene Assays, available for the most common reporter genes, offer chemiluminescent measurement of reporter gene activity that is more sensitive than colorimetric or fluorescent detection. NovaBright™ kits are optimized to yield consistent results with high sensitivity over a wide dynamic range spanning femtogram to nanogram amounts of reporter.
what it offers
- High sensitivity
- Wide dynamic range
- Three easy-to-use formats
how it works
NovaBright™ assays are offered in three easy-to-use formats:
- NovaBright™ Secreted Placental Alkaline Phosphatase (SEAP) Kit
- NovaBright™ β-galactosidase and Firefly Luciferase Dual Enzyme Reporter Gene Chemiluminescent Detection Kit
- NovaBright™ β-galactosidase Enzyme Reporter Gene Chemiluminescent Detection Kit
- Learn More about Chemiluminescence Assays
Detection of secreted placental alkaline phosphatase (SEAP) by the NovaBright™ Secreted Placental Alkaline Phosphatase (SEAP) Enzyme Reporter Gene Chemiluminescent Detection Kit.
|Cat. No.
|NovaBright™ Secreted Placental Alkaline Phosphatase (SEAP) Enzyme Reporter Gene Chemiluminescent Detection Kit
|400 assays
|NovaBright™ Secreted Placental Alkaline Phosphatase (SEAP) Enzyme Reporter Gene Chemiluminescent Detection Kit
|1,200 assays
|NovaBright™ β-galactosidase and Firefly Luciferase Dual Enzyme Reporter Gene Chemiluminescent Detection Kit
|200 assays
|NovaBright™ β-galactosidase and Firefly Luciferase Dual Enzyme Reporter Gene Chemiluminescent Detection Kit
|600 assays
|NovaBright™ β-galactosidase Enzyme Reporter Gene Chemiluminescent Detection Kit for Mammalian Cells
|200 assays
|NovaBright™ β-galactosidase Enzyme Reporter Gene Chemiluminescent Detection Kit for Mammalian Cells
|1,000 assays
|NovaBright™ β-galactosidase Enzyme Reporter Gene Chemiluminescent Detection Kit for Yeast Cells
|200 assays
|NovaBright™ β-galactosidase Enzyme Reporter Gene Chemiluminescent Detection Kit for Yeast Cells
|1,000 assays
Click-iT® Biology Just Got Brighter — New Alexa Fluor® 555 Click-iT® EdU Cell Proliferation Assays and Detection Reagents
what it is
The Alexa Fluor® 555 dye, a superior alternative to tetramethylrhodamine (TRITC) and Cy®3 red-orange fluorophores, is now available as a detection reagent in click chemistry–based detection of cell proliferation and azide/alkyne-labeled biomolecules.
what it offers
- Simply the best fluorescence—brighter and more photostable than TRITC and Cy®3 dyes
- Compatible—enables multiplexed analyses with blue, green, and far-red–fluorescent dyes
- Click-iT® biology—superior alternative to radioactivity- and antibody-based detection
how it works
Click-iT® biology utilizes biologically unique moieties for efficient labeling and detection of molecules of interest using a simple, two-step procedure. In the first step, an azide- or alkyne-containing biomolecule is fed to cells. Unlike other labels, the azide and alkyne tags are small enough that tagged biomolecules are acceptable substrates for incorporation into macromolecules, including proteins, DNA, or RNA. The subsequent detection step utilizes the chemoselective "click" ligation reaction between an azide and alkyne where the modified biomolecule is detected with a corresponding azide- or alkyne-containing dye or hapten. Unlike antibodies (MW ~150 kDa), the Alexa Fluor® 555 Click-iT® detection molecule (MW ~1,000 Da) easily penetrates complex samples.
Alexa Fluor® 555 Click-iT® EdU Cell Proliferation Assay. HeLa cells were treated for 30 min with EdU. Cells were fixed and permeabilized, and EdU that had been incorporated into nascent DNA was detected with the red-orange Alexa Fluor® 555 azide from the Click-iT® EdU 555 Imaging Kit. Tubulin was labeled with a mouse anti-tubulin antibody and visualized with the far-red–fluorescent Alexa Fluor® 647 goat anti-mouse antibody.
|Cat. No.
|Alexa Fluor® 555 azide, triethylammonium salt
|0.5 mg
|Alexa Fluor® 555 alkyne, triethylammonium salt
|0.5 mg
|Click-iT® EdU Alexa Fluor® 555 Imaging Kit *for 50 coverslips*
|1 kit
|Click-iT® EdU Alexa Fluor® 555 HCS Assay *2-plate size*
|1 kit
|Click-iT® EdU Alexa Fluor® 555 HCS Assay *10-plate size*
|1 kit
The Premo™ FUCCI cell cycle sensor enables live-cell imaging of cell cycle and division. As cells progress through the cell cycle, nuclear fluorescence changes from red to green.
what it offers
- Ready-to-use—no need to purify plasmids or worry about vector integrity
- Cell-friendly—no harmful treatments are required; primary and stem cells are labeled without apparent cytopathic effects
- Titratable—defined reagent concentrations enable precise titration and optimization of expression levels
In 2008, Miyawaki and colleagues developed the Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI), a fluorescent protein–based sensor that employs red (RFP) and green (GFP) fluorescent proteins fused to different regulators of the cell cycle: Cdt1 and geminin. The temporal regulation of these proteins by ubiquitin results in the biphasic cycling through the cell cycle. Premo™ FUCCI Cell Cycle Sensor takes this technology one step further by using the BacMam gene delivery system—the prepackaged genetically encoded reagents are ready for immediate use. Simply add the Premo™ FUCCI reagents to your cells, treat with the BacMam enhancer, wash, incubate overnight for protein expression, and visualize cell cycle progression in populations of cells using fluorescence microscopy.
- Learn More about Premo™ FUCCI Cell Cycle Sensor
Imaging cell cycle progression in live cells with Premo™ FUCCI Cell Cycle Sensor. (A) Schematic of cell cycle progression with nuclear fluorescence changes. (B) U2OS cells were transduced with Premo™ FUCCI Cell Cycle Sensor, then stained with Alexa Fluor® 647 wheat germ agglutinin.
what it is
The mitogen-activated protein kinase (MAPK) pathway mediates signal transduction from cell surface receptors to downstream transcription factors that regulate cellular responses such as cell proliferation, growth, motility, survival, and apoptosis. Invitrogen's broad portfolio of products for MAPK research includes both phosphospecific and total antibodies that are validated for multiple applications including western blots, immunoprecipitation, immunocytochemistry, and immunohistochemistry.
what it offers
- Confidence— all phosphorylation site–specificities have been verified with peptide competition
- Performance—robust antibodies validated for multiple applications
- Selection—large menu of unique specificities, with wide range of targets and modification sites
how it works
The role of the MAPK pathway in cancer, immune disorders, and neurodegenerative diseases has been well recognized. Invitrogen now offers phosphospecific antibodies against MEK3, MEK4, and MEK6. MEK3 and MEK6 are members of a tyrosine/threonine protein kinase family that activate p38, which is part of the inflammation/stress signaling pathway. Phosphorylation of MEK3 and MEK6 by MEKK1 activates the proteins and enables them to phosphorylate p38. MEK4 is a member of a tyrosine⁄threonine protein kinase family that activates the c-Jun NH2-terminal kinases (JNK), which is also part of the inflammation⁄stress signaling pathway.
- Products for the MAPK Pathway
- Learn More about Phosphorylation Site–specific Antibodies
- Find Antibodies by Specificity or Application
Phosphospecificity of the MEK3/6 antibody. See larger image for more details
|Species Reactivity*
|Cat. No.
|Hu, Ms, Rt, X
|100 µg
|MEK1 [pS298]
|Hu, Ms, Rt
|10 blots
|MEK2 [pT394]
|10 blots
|MEK3/6 [pS189/pT193]/[207/211]
|10 blots
|MEK4 [pS257/pT261]
|Hu (Ms)
|10 blots
|MEK7 [pS271/pT275]
|Hu (Ms)
|10 blots
|p38 MAPK
|Hu, Ms
|100 µg
|p38 MAPK [pT180/pY182]
|Hu, Rt (Ms, Cn, Mk)
|10 blots
* Cn = canine, Hu = human, Mk = monkey, Ms = mouse, Rt = rat, X = Xenopus. ( ) = reactivity predicted but not tested.
Imaging Mitochondrial Calcium Sequestration — Organelle Lights™ Fluorescent Proteins Meet Classic Molecular Probes® Organelle Stains
The preferential accumulation of the calcium sensor rhod-2, AM in mitochondria has long been utilized to measure calcium flux, an important parameter given the vital role of mitochondria in maintaining intracellular calcium homeostasis. A limitation of using rhod-2 alone is that mitochondria are only visible after calcium uptake; however, determining mitochondrial location prior to the sequestration of calcium can provide important information about the positioning of individual mitochondria with respect to spatially localized intracellular calcium release. For more accurate measurement of mitochondrial calcium flux, the rhod-2 calcium indicator can be combined with probes for observing mitochondrial morphology, such as Organelle Lights™ Mitochondria GFP, for enhanced calcium imaging in individual mitochondria.
Organelle Lights™ reagents are ready-to-use fluorescent protein constructs fused with signal peptides for accurate and specific targeting to sub-cellular compartments and structures. Using the passive mitochondrial marker Organelle Lights™ Mito-GFP, heterogeneities in mitochondrial uptake and important mitochondrial dynamics such as fission, fusion, and motility can be observed before, during, and after the uptake of calcium into mitochondria. Pairing the green-fluorescent Organelle Lights™ Mito-GFP with the red-orange–fluorescent rhod-2, AM calcium sensor allows the spatial and temporal aspects of mitochondrial calcium sequestration to be analyzed with respect to individual mitochondrial dynamics within a given cell and across populations of cells.
- Learn More about Organelle Lights™ Reagents
- Learn More about Products for Mitochondrial Research
|Imaging mitochondrial calcium levels and dynamics with rhod-2, AM and Organelle Lights™ Mito-GFP. (A) HeLa cells were labeled with Organelle Lights™ Mito-GFP and treated with 5 µM rhod-2, AM for 15 min at 37°C. (BI) The region outlined in A is enlarged to show individual mitochondria within a single cell. (BII–III) Calcium release from internal stores following application of 10 µM histamine. Mitochondria in close proximity to the calcium release from internal stores are shown by the increase in the orange-red fluorescence of rhod-2, AM. The arrow in BII denotes a mitochondrion that has impaired mitochondrial calcium uptake, a detail that would not have been revealed using rhod-2, AM alone. The asterisk marks a previously motile mitochondrion that appears to have stopped moving following calcium elevation. Organelle Lights™ Mito-GFP and rhod-2, AM were imaged using standard FITC and TRITC filters, respectively, on a DeltaVision® Core microscope with a 40x objective lens.
High-efficiency Transient Transduction of Human Embryonic Stem Cell–derived Neurons
High-efficiency transient transduction of human embryonic stem cell–derived neurons With baculoviral vectors.
Zeng J, Du J, Lin J et al. (2009) Mol Ther Epub ahead of print.
How can we transiently manipulate human embryonic stem cells to improve transplantation efficiency?
Human embryonic stem cells (hESCs) are an important source of cells for the generation of human neurons for basic research and therapeutic applications. Transplantation of hESC-derived neurons has the potential to repair damaged or diseased neurons, but efforts in this area have been limited by the poor survival and low functional recovery of grafted hESC-derived neurons. Genetic manipulation of hESC-derived cells prior to transplantation is one possible method of improving transplantation efficiency, but chromosomal integration of transgenes poses the risk of permanent expression of potentially harmful genes. Transient transduction is preferable for the expression of genes that are only beneficial during transplantation. To address this challenge, Zeng and colleagues developed a set of baculoviral vectors and demonstrate their use for successful transient transduction of hESC-derived neurons.
To develop a vector optimized for transient transduction of hESC-derived neurons, Zeng and colleagues investigated whether insect baculovirus–based vectors were suitable as a gene delivery system in these cells. They tested various eGFP expression cassettes in addition to the commercially available Organelle Lights™ ER-OFP and Mito-OFP baculoviral systems for transgene expression in hESC-derived neurons and in the brains of nude mice after transplantation.
Baculoviral transduction of hESC-derived neurons was highly efficient (up to 80%), and transgene expression was detected as early as 1 day post-transduction. Transgene expression was stable for at least 1 month in cultured human neurons, and co-transduction of baculoviral vectors with Organelle Lights™ fluorescent protein constructs (using a modified protocol) resulted in successful targeting of the fluorescent proteins concurrent with eGFP transgene expression. Finally, baculovirus-modified hESC-derived neurons were transplanted into the brains of nude mice, where transgene expression was detected for up to 4 weeks after injection. Baculoviral transduction did not appear to inhibit neuronal function, and there was no apparent immune response to the transplanted cells, suggesting that baculoviral vectors are promising tools for the development of more efficient neural transplantation systems.
View bibliography reference
- Learn More about Organelle Lights™ Reagents
|Free online technical webinars
You are invited to join us for a series of biweekly technical webinars from the comfort of your desk. The webinars will initially focus on imaging-related applications, but we welcome your feedback for additional topics throughout the course of the year. Upcoming topics will be announced each month via email.
Presentations will last approximately 45 minutes, followed by 15 minutes for live Q&A.
Missed our previous webinars? Find our recorded webinars here!
Live-cell imaging of cell cycle and division. U2OS cells were transduced with Premo™ FUCCI Cell Cycle Sensor, then imaged the next day using a 40x objective and standard FITC/TRITC filter sets. Time lapse imaging was performed on the DeltaVision® Core microscope. Images were collected every 15 min for 18 hr.
Watch the Video!
Epitope Tag Antibodies
Over the years, a number of different epitope tags have been made available to researchers studying protein expression. The tags are recognized by antibodies—which can in turn be visualized by standard secondary detection techniques—and have proven useful for detecting proteins of interest via western blotting, ELISA, immunostaining, and immunoprecipitation. Invitrogen offers antibodies against several of the most popular epitope tags, including V5, Xpress, myc, histidine (his), and hemagglutinin (HA). Each antibody is also available in several conjugate forms and includes complete protocols for use.
- Browse our complete offering of Epitope Tag Antibodies
Myc and V5 tags detected using epitope tag antibodies. (A) Myc tag detected with anti-myc antibody. (B) V5 tag detected with anti-V5 antibody.
| Product
|Cat. No.
|Anti-V5 Antibody
|50 µL
|Anti-Xpress™ Antibody
|50 µL
|Anti-myc Antibody
|50 µL
|Anti-histidine (c-terminal) Antibody
|50 µL
|Anti-HA Antibody
|100 µg
|Environmental Sustainability Programs
Invitrogen, part of Life Technologies, is dedicated to protecting the environment. We have ongoing environmental sustainability programs that deliver products to reduce your environmental footprint, while helping you achieve your research goals.
|New Web Resource for IDC Latex Microspheres
Invitrogen offers a wide selection of UltraClean™ surfactant-free latex microspheres—also referred to as latex beads—for research and commercial applications. We have recently redesigned our IDC latex microspheres web page to include more information about the different surface modifications, detailed background information on the physical properties of microspheres, and a guide to selecting the best microsphere for your application. Also included are protocols for attaching latex microspheres to proteins or other biomolecules.
Post-Sorting Analysis Enabled by Low Toxicity of Cell Cycle Stain
Vybrant® DyeCycle™ Ruby Stain is used for cell cycle analysis in live cells by flow cytometry using the common 635 nm red laser for excitation. In collaboration with Derek Davies and Tina Luke from the FACS Laboratory at London Research Institute-Cancer Research UK, Invitrogen scientists have shown that Vybrant® DyeCycle™ Ruby Stain has low toxicity in live cells. This means that cells stained with Vybrant® DyeCycle™ Ruby Stain can be sorted on a flow cytometer into different phases of the cell cycle and then re-grown for subsequent analyses.
- Learn More about Vybrant® DyeCycle™ Ruby Stain
Post-sorting analysis of cell number and viability. HL-60 cells (5 x 10 6) were suspended in 10 mL of medium, 10 µL Vybrant® DyeCycle™ Ruby Stain was added, and the mixture was incubated for 15 min at 37°C. The cells were pelleted and resuspended in 1 mL PBS with 5 µL DAPI (200 µg/mL). ( A) Live gating on DAPI negative cells followed by singlet gating based on area and height and sorted G 0/G 1 population (675/20 blue laser excitation and 660/20 red laser excitation). Mod FIT LT software was used to model the cell cycle, and the G 0/G 1 cells were then re-grown and evaluated for cell count ( B) and viability ( C).
|Cat. No.
|Vybrant® DyeCycle™ Ruby Stain
|400 assays
|Vybrant® DyeCycle™ Ruby Stain
|100 assays
Molecular Probes® The Handbook
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Spring winged aircraft described in ScienceNOW, 19 January 2005. Insects use two layers of muscle to flap their wings - as each layer contracts it stretches the other, which then contracts whilst the other layer relaxes and gets stretched ready for the next cycle. This process occurs too quickly to be controlled by nerve impulses, so scientists have proposed that it works by using energy stored in the muscles themselves. A team of scientists led by Thomas Irving, a biophysicist at the Illinois Institute of Technology was able to follow the movement of proteins in fly wing muscles using a high intensity x-ray beam. They found that a long rod shaped molecule named myosin, that was previously thought to be stiff, was actually stretched between muscle contractions so it could store energy like a stretched spring. According to ScienceNOW this study "may have finally solved the mystery of how insects flap their wings so fast".
Editorial Comment: This kind of precision engineering at the molecular level is a powerful challenge to the idea that proteins evolved by random changes in their amino acid sequences. Unless the myosin proteins had the right 'springy' properties to start with, the insects would not be able to fly, and would lose out in any struggle to survive. (Ref. flight, proteins, nanotechnology)


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One of my tasks here at Concord as the Youth Outreach Director is to help design our website. Because of this, I am always looking for new ways to present our message and I pay close attention when Concord's message appears in the many different media publications our staff has the fortune of being featured and interviewed in. Readers can normally keep up-to-date on these appearances at our "Concord in the News" page for traditional print articles or by clicking on the "Video and Audio" section of that page for multimedia.
I got quite excited recently because something came along that didn't fit neatly into these categories. Concord Coalition Policy Director Josh Gordon was interviewed about the scale and the nature of our nation's fiscal challenge, as well as how we can go about addressing it, by an organization called FLYP Media. They have a new bi-weekly publication that is part magazine, part TV show, and part in-depth and content rich website for a well-designed and well-integrated, truly multi-media experience.
I think that experimenting with such innovation in getting information to the American public is a crucial element in having messages like ours--for generationally responsible fiscal policy, and all of the difficult and complex choices that it entails--reach and persuade the American public. I hope to help Concord do this, even if it is little-by-little, over the coming months and years.
--Stefan Byrd-Krueger
External links:FLYP Media Article About Concord


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Join me on Tuesday, March 19th, 2013 at 6:30 PM EST as I help co-host the P&G Orgullosa "Skirts Only" Twitter Party. We'll be chatting during an exclusive LIVESTREAM "Skirts Only" Fashion Show with designer Cenia Paredes straight from New York City!
P&G Orgullosa looks to celebrate MUJERES CON LA FALDA BIEN PUESTA™! Which translates to "women who have their skirts well placed." That means a strong and confident Latina woman to me.
Orgullosa is a new initiative that celebrates the diverse collective beauty of Latina women. The movement encourages Latina women to promote not only our own beauty standard, but also our accomplishments as daughters of the new world.
The show will feature a red carpet welcome, skirts designed by Cenia Paredes' line CENIA®, and celebrity guest appearances! Models, spokespersons for Orgullosa (ABC's Evette Rios, motivational speaker Maria Marin, and chocolatier Maribel Lieberman) and other women will walk the runway. View the Facebook event page here.
We'll be tweeting right along with the fashion show talking about all things style and beauty for spring! Pop singer Jasmine V will serve as our celebrity co-host! And there will be amazing prizes, so make sure you RSVP here to be eligible to win. Get all the Twitter party details below.
Twitter Party Details:
What: P&G Orgullosa "Skirts Only" Twitter Party
When: Tuesday, March 19th at 6:30 PM EST
Where: Twitter
Hashtag: #LaFaldaBienPuesta
Who to Follow:
Celebrity Host: Jasmine Villegas: @JasmineVillegas
Co-Hosts: @DiarioDeLaModa, @BeChicMag, @CURVESandCHAOS, @SocialRosy, @NinaSays
Moderators: @LatinaMomBlogs, @JollyMom, @TexinMiami, @LatinoMarketing
Brand: @Orgullosa
Winners will be selected at random, for a total of five (5) winners:
– One (1) off-the-runway skirt of your choice (customized to fit) by designer CENIA
– One (1) $300 gift card to start a new wardrobe and an Orgullosa branded gift bag full of P&G beauty products
– One (1) $100 gift card and a branded gift bag full of P&G beauty products
– Two (2) $50 gift cards and a branded gift bag full of P&G beauty products
Rules: For the official rules, please click HERE.
RSVP: To be eligible to win prizes, you must RSVP by entering your Twitter handle and Twitter handle URL HERE.
Disclosure: This is part of a paid campaign with Latina Mom Bloggers. Any opinions expressed in this post are 100% my own.


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Cops: Man killed roommate for stealing food
Published: Tuesday, November 27, 2012 at 1:48 p.m.
Last Modified: Tuesday, November 27, 2012 at 1:48 p.m.
ST. PETERSBURG, Fla. — A southwest Florida man has been charged with second-degree murder after authorities say he fatally stabbed his roommate for stealing his food.
St. Petersburg Police said 36-year-old Corey Walker was angry on Tuesday morning, telling his roommates that someone was stealing food from his room. He blamed Anthony Smith and grabbed a butcher knife from the kitchen and allegedly stabbed Smith once in the chest.
Another roommate flagged down a passer-by and asked them to call police.
The 56-year-old Smith died at the hospital.
The two men live at a transitional house run by a local church.
Walker was arrested at the home without incident. The knife was recovered in the kitchen sink.
Reader comments posted to this article may be published in our print edition. All rights reserved. This copyrighted material may not be re-published without permission. Links are encouraged.


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The Number PatternTrivia brain teasers have some element of trivia in them, but they are not just pure trivia questions.
When is the following true?
Add 7 more and it becomes 13.
AnswerIn a game of Black Jack.
An ace is originally counted as 11 points, but if a card is received that causes the total to go over 21, the ace can be converted to one point.
What Next?
See another brain teaser just like this one...
Or, just get a random brain teaser
If you become a registered user you can vote on this brain teaser, keep track of
which ones you have seen, and even make your own.
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