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PR / Press and Communications Officer
- Recruiter
- eRecruit Solutions Ltd
- Location
- Colchester Essex
- Salary
- £24-30,000 plus excellent benefits
- Posted
- 07 Oct 2013
- Closes
- 07 Nov 2013
- Ref
- Function
- Press Officer
- Hours
- Full Time
- Contract Type
- Permanent
A PR / Press and Communications Officer is sought to join this high profile registered Charity in Colchester, Essex. The organisation provides direct, practical support to wounded, injured and sick Service personnel, veterans and their families. They do this through grants to individuals and other Service charities, capital build projects and support at their four Recovery Centres cross the UK.
TITLE: PR / Press and Communications Officer
LOCATION: Colchester, Essex
BENEFITS: Staff Discount on Merchandise, Healthcare, Pension, Life Assurance, Enhanced Holiday Entitlement, Free Parking
The ideal PR / Press and Communications Officer will possess:
- 2-3 years' experience working on high profile media campaigns and dealing with national/regional press
- The ability to deliver multiple projects to strict deadlines
- High level of attention to detail and great organisational skills
- Charity or not-for-profit sector experience (highly desirable but not essential)
- Experience of social and digital media is desirable
- Must live or be able to relocate to Colchester
To succeed in this role as a PR / Press and Communications Officer you will be expected to:
- Deliver the charity and Recovery Centres media plans in an effective manner
- Be responsible for identifying, developing and exploiting corporate relationships and recognise opportunities to raise the public profile and reputation of the charity
- Responsible for driving media coverage to ensure key messages of the charity are communicated effectively and consistently
- Develop relationships with key local and regional journalists to gain media exposure across all channels
- Develop a database of relevant images, video and media collateral for use by the charity
As a PR / Press and Communications Officer you will be joining an established Charity offering you an opportunity to get involved and "do your bit". They offer an excellent working environment with a relaxed dress code and free parking.
Please click to apply now for this role as a PR / Press and Communications Officer in Colchester, Essex!
If the details shown on your CV match our client's requirements, we will provide our client with your full details so that they can judge whether you may be suitable for the opportunity advertised. Your details, as submitted by you, will only be used in relation to this specific opportunity. By applying to this advert and submitting your CV and/or cover letter to us, you give express consent to us using your details for this purpose.
eRecruit Solutions is a flat fee online recruitment company that actively recruit for the following roles: PR Officer, Public Relations Officer, Press & Media Assistant, Communications Officer, Marketing Manager in Colchester, Essex.


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Major requirements
Italian Studies major worksheet
The major in Italian studies consists of at least nine courses (starting with ITAS 200 or above). At least four of the nine courses must be in Italian.
Nine courses from the following:
ITAS 200 Advanced Intermediate Italian
ITAS 220 Advanced Oral and Written Communication: Composition and Conversation
ITAS 235 Italian Women Writers in Translation
ITAS 260 Italian Civilization
ITAS 305 Infernos, Brothels and Courts
ITAS 306 Studies in Italian Literature II
ITAS 320 Italian Cinema
ITAS 330 Modern Italian Theatre
ARTH 231 Italian Medieval Art and Culture
ARTH 232 Art and Architecture of the 14th and 15th Centuries in Italy
ARTH 274 Visualizing Ancient Rome
ITAS 310 Fashion, Sex and the City
ARTH 401 Seminar
Other combinations are also possible, if students take advantage of special courses that deal directly with Italy and/or Italians in America offered by other departments. Such courses will vary from year to year.
We also encourage majors to spend their junior year abroad with an approved program, such as Middlebury in Florence or Ferrara and the Institute for the International Education of Students (IES) in Milan or Rome.
Italian Studies majors will complete their major capstone experience in their senior year. The major capstone experience in Italian Studies will consist of advanced work in the field or in an interdisciplinary area of the field. Senior majors can take an upper level experimental Itas 398 course, when offered; engage in original and independent research by requesting an Itas 399; or take an enhanced version of an upper level Italian course offered in their senior year. The learning outcome will generally consist of a long research paper in Italian in a literary or interdisciplinary area of student interest.

subset: commoncrawl


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By: Asma Stephan
Twitter has expanded their ad program with the use of interest targeting. While Promoted Tweets and Promoted Accounts give brands some ability to target users, the potential reach of these campaigns was limited to Tweeters who shared interests with current followers.
According to today's announcement on the Twitter blog, interest targeting will not only help brands broaden their reach, but also more precisely target their audience.
For a broad reach, brands can target users using a comprehensive list of over 350 different interest categories, such as Home and Garden, or Style and Fashion or Hobbies and Interests.
This puts Twitter's ad campaigns in a better position to compete with Facebook's targeted ad offering, which features a similar checklist of categories. Brands who've seen success targeting niche audiences on Facebook would do well to incorporate interest targeting on Twitter.
Alternatively, brands can target a custom segment of specific Tweeters and any followers with shared interests. They can specify any relevant Twitter handles associated with the product, event, or initiative the brand is interested in promoting in order to reach a tight-knit network of Twitter users.
Targeting a set of users this way offers an especially provocative possibility: allowing brands the ability target their competition's followers.
Though Twitter hasn't released any hard data about the success of interest targeting, they report promising results, saying:
"[Beta advertisers] have seen significantly increased audience reach; others have creatively defined custom audiences to reach a very specific set of users. Across the board, they are seeing high engagement rates because they are reaching users who are interested in their content."
Precisely reaching an audience with highly customized, highly targeted promotions is the holy grail of social media marketing. Twitter's latest offering could prove to be a huge step in the right direction for brands.
What is your take on Twitter's interest targeting? Do you already use Promoted Tweets and Promoted Accounts? Does this launch make you more likely to create a Twitter marketing campaign? Tell us in the comments below!


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SPRING, Texas - A four-car pileup led to two arrests after deputies found marijuana in one of the cars involved in the crash.
The wreck happened while several cars headed west on FM 1960 in Spring were all stopped at the Cypress Station light around 12:20 a.m. on Saturday.
Harris County deputies said an SUV slammed into the back of a Cadillac, then the Cadillac ran into the car in front of it and sideswiped a fourth vehicle.
According to reports, the SUV flipped on its side and the male driver had to be pulled out of the car by rescue crews. He was transported by Life Flight to the hospital in critical condition.
The two occupants in the Cadillac--a man and a woman--were also taken to the hospital in serious but stable condition.
Deputies said the two men in the small car that was hit by the Cadillac were treated at the scene and later arrested after deputies found a large amount of marijuana in their car.
Copyright 2013 by Click2Houston.com. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


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We believe no other online retailer can match our combination of low prices, product presentation, customer service, and product guarantees.
Our competition attempts to compete by offering super low prices with no support, while we devote our time to exceptional customer service, accurate descriptions and photography for each product, and providing answers to each email we receive.
Even with the significant differences between us and our "low-price" competition, we vow to be the low-price leader.
If you find an identical item with a lower price, we will match and lower the price for you! Complete our lowest price request form and we will discount our price by 105% of the difference!
|Eligibility Requirements
Each request must meet the following conditions:
We reserve the right to limit our lowest price policy if the Internet retailer:
Other conditions:
<< Return to low price request form


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Popular TV Shows 5Trivia brain teasers have some element of trivia in them, but they are not just pure trivia questions.
Below are five (5) anagrams of actors from a popular 1960s TV show. Your job is twofold: firstly, name these people and secondly, ascertain the TV show they all featured in. I have included the number of letters of each actor's name in brackets.
Choral junket (4, 8)
Market trains (5, 7)
Drag odd mark (4, 7)
Wail slim guy (3, 8)
An ash or thin jar (8, 6)
Hint"Now come along with me, you ludicrous lump, there's much to be done."
"Silence, you mental midget."
"Yours not to question why; yours to do as I say or die."
AnswerJune Lockhart
Marta Kristen
Mark Goddard
Guy Williams
Jonathan Harris
The TV series: Lost in Space (1965-68)
The cast:
June Lockhart - Maureen Robinson
Marta Kristen - Judy Robinson
Mark Goddard - Maj. Don West
Guy Williams - Prof. John Robinson
Jonathan Harris - Dr. Zachary Smith
What Next?
See another brain teaser just like this one...
Or, just get a random brain teaser
If you become a registered user you can vote on this brain teaser, keep track of
which ones you have seen, and even make your own.
Back to Top


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The Crap We Missed – Monday 9.17.12
Welcome to Monday's The Crap We Missed, where we take a weekend's worth of celebrity nonsense and transmute it into pure gold. It's just like alchemy, only the gold is dick jokes. Anyway, today we've got Wilmer Valderrama showing us his swag bag of high tech gadgets that he will trade to teenagers for sex, while Dolph Lundgren opts for a much more direct approach, Victoria Beckham letting baby Harper know what's will happen if that double chin isn't gone by her first birthday, this vampire, who will know haunt your dreams for the next eternity or so, and finally, Mark-Paul Gosselaar's epic fail at Hamming (I told you).
Holy Cow! Is that a recognizable person in the Final Five? You'll have to forgive me, it's unusual is all..
- Photo Boy
Click Here To Start The Gallery
Photo: Bauer-Griffin, Fame/Flynet, Getty, INFdaily, Pacific Coast News, Splash News, WENN


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Iowa's Historic Hills Scenic Byway
Autumn color paints the valleys and family farms between the Villages of Van Buren County, seven historic burgs punctuating this quiet 110-mile drive along the Des Moines River in southeast Iowa. During the Scenic Drive Festival in October, craft vendors and friendly pancake feeds add to the small-town appeal.
Creative class In Bentonsport, the husband-wife team of Bill and Betty Printy (aka Iron and Lace) draw scores of art-lovers with her Queen Anne's lace pottery and his hand-forged metal pieces. Watch them work during the fest.
For maps and trip planning
Historic Hills Scenic Byway [1]


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Picture a hip-hop music video, except images of drinking and partying are replaced with studying, and scantily clad back-up dancers are replaced with University at Albany cheerleaders.
That's the video a couple of SUNY alumni made to help market the 64-campus system. Chancellor Nancy Zimpher showed the nearly-four minute video as the opening to her State of the University address Tuesday.
The video will be released to the public next month, but if you bear with our "video of a video" from the speech, you can get a taste.
Here's "If You Go to SUNY," performed by UAlbany graduate Brendan Martin, who studied business and was recently signed with Warner Music. His agent, Jason Singer, is also a UAlbany alum.


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Ford Transit-350 HD Repair EstimatesChoose from 87 Ford Transit-350 HD repairs and services for model years 2015 to 2017 to get a RepairPal Fair Price Estimate™.
Learn more
RepairPrice Estimates are based on the estimated time for the repair, labor rates charged by trained automotive professionals, and prices for quality parts.
These estimates are designed to show a range of fair prices, instead of a single price, because there is more than one part that is appropriate to use in each repair, and more than a single mechanic who is qualified to do the repair.
Each estimate is customized for your vehicle and geographic area. Estimates exclude applicable taxes, diagnostic charges and disposal fees.
All Ford Transit-350 HD Repairs and Services
- Maintenance Schedule
- ABS System Diagnosis
- Air Conditioning Diagnosis
- Air Conditioning Expansion Valve Replacement
- Air Conditioning System Leak Inspection
- Air Filter Replacement
- Automatic Transmission Diagnosis
- Battery Cable Replacement
- Battery Replacement
- Brake Fluid Replacement/Flush
- Brake Hydraulic System Bleed
- Brake Light Switch Replacement
- Brake Pad Replacement
- Brake Pad Replacement, Resurface Rotors
- Brake Rotor Replacement
- Cabin Air Filter Replacement
- Charging System Diagnosis
- Check Engine Light Diagnosis
- Coolant Leak Diagnosis
- Cooling System Leak Inspection
- Differential Fluid Replacement - Rear
- Drive Axle Assembly Leak Inspection
- Drive Belt Idler Pulley Replacement
- Drive Belt Tensioner Replacement
- Emergency Brake Cable Replacement
- Engine Control Module (ECM) Replacement
- Engine Front Cover Gasket Replacement
- Engine Leak Inspection
- Engine Mount Replacement
- Engine Oil Light Diagnosis
- Engine Oil Pan Gasket Replacement
- Engine Oil Pan Replacement
- Engine Overheating Diagnosis
- Exhaust Muffler Replacement
- Fuel Evaporative Canister Replacement
- Fuel Filter Change
- Fuel Level Sending Unit Replacement
- Fuel Tank Replacement
- General Diagnosis
- Glow Plug Replacement
- Headlamp Bulb Replacement
- Headlamp Switch Replacement
- Horn Replacement
- No Start Diagnosis
- Noise Diagnosis
- Oil Leak Diagnosis
- Oil and Filter Change
- Power Steering System Leak Inspection
- Pre-Purchase Car Inspection
- Serpentine Belt Replacement
- Spark Plug Replacement
- Stabilizer Bar Link Kit Replacement
- Stabilizer Bushing Replacement
- Steering Knuckle Replacement
- Suspension Control Arm Replacement
- Thermostat Replacement
- Throttle Body Replacement
- Timing Chain Tensioner Replacement
- Tire Rotation
- Trans Leak Inspection
- Trans Oil Cooler Line Replacement
- Transmission Fluid Change
- Variable Valve Timing Control Solenoid Replacement
- Vibration Diagnosis
- Wheel Alignment
- Wheel Bearing Replacement
- Wheel Hub Assembly Replacement
- Wheel Lug Stud Replacement
- Wheel Speed Sensor Replacement
- Window Switch Replacement
- Windshield Washer Fluid Reservoir Replacement
- Windshield Wiper Arm Replacement
- Windshield Wiper Blade Replacement
High Quality RepairsYour auto repair done right, only the work you need with no add ons.
Never OverpayOur free estimator calculates a custom price for your vehicle repair.
Guaranteed RepairsRest easy knowing you're covered by our RepairPal Nationwide Warranty.


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Over the next few days we'll be posting various videos from the DIY DAYS LA event. The day consisted of a number of keynotes (Robert Greenwald, Marshall Herskovitz), panels (Tommy Pallotta, Femke Wolting, Alex Johnson, Micki Krimmel, Mark Stolaroff, Ondi Timoner, Hunter Weeks, Saskia Wilson-Brown), case studies (M dot Strange, Arin Crumley, Lance Weiler), a series of special video presentations (Matt Hanson, Brett Gaylor, Brian Chirls, Christy Dena, Timo Vuorensola) and a conversation with director Mark Pellington.
M dot Strange, Hunter Weeks and Ondi Timoner – photo by Mike Hedge
The Realities of DIY
There's been much discussion about the democratization of the tools but what's really involved in taking your film from a concept to something an audience will pay to see? How can you fight your way through the clutter and what are the pitfalls to avoid when you decide to go it on your own?
Discussion Leader: Mark Stolaroff – panelists Arin Crumley, Ondi Timoner, Hunter Weeks and M dot Strange.BTS DIYDays animation audience biz case study deals discussion distro diy doc dvd education event festivals funds how to narrative online panel podcast producing production promotion resource sponsorship tech theatrical tools tv web 2.0


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A CHARITY fancy dress party has been organised in support of Worcester's breast unit.
The party, organised by Sainsbury's in St John's, Worcester is scheduled to take place in the Bank House Hotel in Bransford, near Worcester on Sunday, September 7 in support of the Worcestershire Breast Unit Campaign.
Everyone is welcome and people can attend from 4pm with the party expected to last until late. Guests do not have to come in fancy dress but there will be prizes for the best fancy dress costume.
The proceeds will go towards creating the unit at 220 Newtown Road, Worcester. The charity, supported by the Worcester News since the launch of the campaign in October 2009, seeks to create a one-stop shop for breast care patients so they can get the majority of their treatment in one place, within the county and with their privacy, dignity and comfort paramount. Existing clinics are spread out over different floors at Worcestershire Royal Hospital which is not always convenient for patients. The new unit would be designed with input from patients so it better corresponds to their needs.
Tickets which cost £5 are available from the customer service desk at the store in Swanpool Walk, St John's or by contacting Louise Shaw on 01905 683800 or by emailing firstname.lastname@example.com


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View unanswered posts | View active topics
Board index » Question and Answer » Sharpening Q&A
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Michiel Vanhoudt wrote:Some of you may know I designed a couple of knives in the past. Mostly folders and comping and outdoors knives. I have asked some makers to alter a few specs when it came to kitchen knives, but never did I design a kitchen blade from scratch like I want it to be.Here it is!http://belgiansharpening.wordpress.com/ ... hsi-blade/
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The warm glow and elegance of Metallaire™ decorative ceiling tiles create an enticing ambiance that's hard to resist. These panels are made to look like the historic tin ceiling tiles of the late 19th century. Add a touch of sparkle and charm to any room with one of 12 classic tin ceiling designs that include medallions, florals, and trefoils.
Metallaire decorative ceiling tiles are made of real metal and won't rust. Finishes include white, chrome, brass, copper, and paintable lacquered steel, so you can custom-match the colors in your room.
Panels Sizes and Accessories
Metallaire tin ceiling tiles come in either a 24" x 24" lay-in panel for use in suspended grid systems, or 24" x 48" for nail-up installation.
Five tin ceiling styles (small panels, fans, small floral circle, vine, and medallion) are also available in the smaller 18½" x 48½" size for use as a backsplash.
Accessories come in all 5 finishes:
Decorating with Metal Ceiling Tiles
The glamorous look of tin ceiling tiles will transform your plain-Jane ceiling by adding color, texture, and flair. No matter what your décor style – from fabulous antique or vintage to modern chic – Metallaire decorative ceilings make everyone look up and take notice.
Read More About Antique Ceiling Tile Ideas
Decorating Tips
DIY Installation
Pressed metal ceiling tiles are more affordable and easier to install than yesteryear's tin ceilings. Their DIY-friendly design let's you complete an entire ceiling in as little as a weekend. Our installation guides and how-to videos give you complete instructions. For inspiration, visit our photo gallery.


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Generic Name: fondaparinux (fon-DAP-a-rin-ux)
Brand Name: Arixtra
Tell your doctor you use fondaparinux before you have any type of spinal or epidural procedure. Patients who have these procedures while they use fondaparinux are at risk for developing bleeding around your spine. This could result in long-term or permanent paralysis.
The risk is increased in patients who have an indwelling epidural catheter. It is also increased in patients with a history of traumatic or repeated epidural or spinal puncture, a deformed spine, or spinal surgery. It is also increased in patients who use medicines that may affect blood clotting, such as other anticoagulants (eg, warfarin), aspirin, platelet inhibitors (eg, clopidogrel), and nonsteroidal anti-inflammatory drugs (NSAIDs) (eg, ibuprofen, naproxen).
If you have a spinal or epidural procedure while you use fondaparinux, tell your doctor at once if you notice any symptoms of nerve problems (eg, back pain; numbness or tingling, especially in the legs and feet; muscle weakness; paralysis; loss of bladder or bowel control).
Talk with your doctor if you have recently had or will be having a spinal or epidural procedure. Some time may need to pass between the use of fondaparinux and your procedure. Discuss any questions or concerns with your doctor
Fondaparinux is used for:
Preventing blood clots in patients who will be having certain types of surgery. Fondaparinux is also used with warfarin to treat blood clots in the leg or lungs. It may also be used for other conditions as determined by your doctor.
Fondaparinux is an anticoagulant. It works by inhibiting one of the steps necessary for the formation of blood clots, which helps prevent clots from developing or worsening.
Do NOT use fondaparinux if:
- you are allergic to any ingredient in fondaparinux
- you have severe kidney problems, inflammation of the heart due to a bacterial infection (eg, bacterial endocarditis), active major bleeding, or severe bleeding problems
- you have a low platelet count and the presence of antiplatelet antibodies
- you weigh less than 110 pounds (50 kg) and are using fondaparinux to treat a blood clot in the lungs or legs
- you weigh less than 110 pounds (50 kg) and will be having hip or knee replacement surgery or abdominal surgery, or you have had a hip fracture
Contact your doctor or health care provider right away if any of these apply to you.
Before using fondaparinux:
Some medical conditions may interact with fondaparinux. Tell your doctor or pharmacist if you have any medical conditions, especially if any of the following apply to you:
- if you are pregnant, planning to become pregnant, or are breast-feeding
- if you are taking any prescription or nonprescription medicine, herbal preparation, or dietary supplement
- if you have allergies to medicines, foods, or other substances (eg, latex)
- if you have a history of kidney problems, liver problems, stomach or intestinal problems (eg, ulcers), stroke, or vision problems caused by diabetes
- if you have a bleeding disorder; a history of brain bleeding or blood conditions; severe, uncontrolled high blood pressure; or low body weight
- if you have recently had or are scheduled to have brain, spine, or eye surgery; an epidural catheter; or a spinal puncture
- if you are taking medicines that affect platelets (eg, aspirin, clopidogrel, ticlopidine)
Some MEDICINES MAY INTERACT with fondaparinux. Tell your health care provider if you are taking any other medicines, especially any of the following:
- Activated protein C (eg, drotrecogin alfa), anticoagulants (eg, warfarin), direct factor Xa inhibitors (eg, rivaroxaban), direct thrombin inhibitors (eg, dabigatran), NSAIDs (eg, ibuprofen, ketorolac, naproxen), platelet inhibitors (eg, clopidogrel, ticlopidine), or salicylates (eg, aspirin) because they may increase the risk of bleeding
This may not be a complete list of all interactions that may occur. Ask your health care provider if fondaparinux may interact with other medicines that you take. Check with your health care provider before you start, stop, or change the dose of any medicine.
How to use fondaparinux:
Use fondaparinux as directed by your doctor. Check the label on the medicine for exact dosing instructions.
- An extra patient leaflet is available with fondaparinux. Talk to your pharmacist if you have questions about this information.
- Fondaparinux is usually given as an injection at your doctor's office, hospital, or clinic. If you will be using fondaparinux at home, a health care provider will teach you how to use it. Be sure you understand how to use fondaparinux. Follow the procedures you are taught when you use a dose. Contact your health care provider if you have any questions.
- Do not use fondaparinux if it contains particles, is cloudy or discolored, or if the syringe is cracked or damaged.
- Use the proper technique taught to you by your doctor. Inject deep under the skin, NOT into muscle.
- Use a different injection site each day to prevent problems at the injection site (eg, hematomas).
- Keep this product, as well as syringes and needles, out of the reach of children and pets. Do not reuse needles, syringes, or other materials. Ask your health care provider how to dispose of these materials after use. Follow all local rules for disposal.
- If you miss a dose of fondaparinux, use it as soon as possible. If it is almost time for your next dose, skip the missed dose and go back to your regular dosing schedule. Do not use 2 doses at once.
Ask your health care provider any questions you may have about how to use fondaparinux.
Important safety information:
- Fondaparinux may reduce the number of clot-forming cells (platelets) in your blood. Avoid activities that may cause bruising or injury. Tell your doctor if you have unusual bruising or bleeding. Tell your doctor if you have dark, tarry, or bloody stools.
- Tell your doctor or dentist that you take fondaparinux before you receive any medical or dental care, emergency care, or surgery.
- Before you begin taking any new prescription or nonprescription medicine, check the label to see if it has aspirin or ibuprofen in it. If it does or you are not sure, contact your doctor or pharmacist.
- Fondaparinux may affect certain lab tests, including liver enzyme. Be sure your doctor and lab personnel know you are using fondaparinux.
- Lab tests, including complete blood cell counts (eg, platelet counts), kidney function, and stool blood, may be performed while you use fondaparinux. These tests may be used to monitor your condition or check for side effects. Be sure to keep all doctor and lab appointments.
- Use fondaparinux with caution in the ELDERLY; they may be more sensitive to its effects, especially bleeding.
- Fondaparinux should be used with extreme caution in CHILDREN; safety and effectiveness in children have not been confirmed. Children may have an increased risk of bleeding with fondaparinux.
- PREGNANCY and BREAST-FEEDING: If you become pregnant, contact your doctor. You will need to discuss the benefits and risks of using fondaparinux while you are pregnant. It is not known if this medicine is found in breast milk. If you are or will be breast-feeding while you use fondaparinux, check with your doctor. Discuss any possible risks to your baby.
Possible side effects of fondaparinux:
All medicines may cause side effects, but many people have no, or minor, side effects. Check with your doctor if any of these most COMMON side effects persist or become bothersome:
Seek medical attention right away if any of these SEVERE side effects occur:
Mild bleeding, irritation, rash, or itching at the injection site; trouble sleeping.
Severe allergic reactions (rash; hives; itching; difficulty breathing; tightness in the chest; swelling of the mouth, face, lips, or tongue); bloody, black, tarry stools; confusion; difficulty walking; fainting; fever; increased drainage or oozing from a wound; loss of appetite; nosebleed; one-sided weakness; pale skin; pink or red urine; severe or persistent dizziness or headache; severe or persistent tiredness, weakness, nausea, or vomiting; slurred speech; swelling of the ankles, feet, or hands; tingling or numbness (especially in the legs and feet), and muscle weakness; unusual or severe bleeding or bruising; vomit that looks like coffee grounds.
This is not a complete list of all side effects that may occur. If you have questions about side effects, contact your health care provider. Call your doctor for medical advice about side effects. To report side effects to the appropriate agency, please read the Guide to Reporting Problems to FDA.
If OVERDOSE is suspected:
Contact 1-800-222-1222 (the American Association of Poison Control Centers), your local poison control center, or emergency room immediately. Symptoms may include excessive bleeding and bruising.Proper storage of fondaparinux:
Store fondaparinux at 77 degrees F (25 degrees C). Brief storage at temperatures between 59 and 86 degrees F (15 and 30 degrees C) is permitted. Store away from heat, moisture, and light. Do not store in the bathroom. Keep fondaparinux out of the reach of children and away from pets.
General information:
- If you have any questions about fondaparinux, please talk with your doctor, pharmacist, or other health care provider.
- Fondaparinux is to be used only by the patient for whom it is prescribed. Do not share it with other people.
- If your symptoms do not improve or if they become worse, check with your doctor.
- Check with your pharmacist about how to dispose of unused medicine.
This information should not be used to decide whether or not to take fondaparinux or any other medicine. Only your health care provider has the knowledge and training to decide which medicines are right for you. This information does not endorse any medicine as safe, effective, or approved for treating any patient or health condition. This is only a brief summary of general information about fondaparinux. It does NOT include all information about the possible uses, directions, warnings, precautions, interactions, adverse effects, or risks that may apply to fondaparinux. This information is not specific medical advice and does not replace information you receive from your health care provider. You must talk with your healthcare provider for complete information about the risks and benefits of using fondaparinux.
Disclaimer: This information should not be used to decide whether or not to take this medicine or any other medicine. Only your health care provider has the knowledge and training to decide which medicines are right for you. This information does not endorse any medicine as safe, effective, or approved for treating any patient or health condition. This is only a brief summary of general information about this medicine. It does NOT include all information about the possible uses, directions, warnings, precautions, interactions, adverse effects, or risks that may apply to this medicine. This information is not specific medical advice and does not replace information you receive from your health care provider. You must talk with your healthcare provider for complete information about the risks and benefits of using this medicine.
More about fondaparinux
- Side Effects
- During Pregnancy
- Dosage Information
- Drug Interactions
- Support Group
- Pricing & Coupons
- En Español
- 5 Reviews – Add your own review/rating
- Drug class: factor Xa inhibitors
Consumer resources
Other brands: Arixtra


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Hey there, time traveller!
This article was published 28/10/2013 (1393 days ago), so information in it may no longer be current.
I was dreading it.
Dreading the thought of being in front of nearly 1,400 people and having to walk the fashion runway at Sunday's 22nd annual Guardian Angel Benefit for Women's Cancer.
Until, that is, I got to meet and got to know the other cancer survivors who walked the fashion runway with me. Woman and men and even teenagers who have walked the walk with various forms of the disease in ways far more challenging than my own.
There was 78-year-old Ethel Gordon, a breast cancer survivor of nearly 20 years who delighted the crowd by boogying down the RBC Convention Centre Winnipeg runway. And 74-year-old former Canadian men's curling champion Terry Braunstein modelling pyjamas almost as bravely as he has confronted and beaten bladder and prostate cancer. And Ryan Veldkamp, who's just 16, and Jill Zarney, who's 26 and Erin Smith who's 29.
And then there was Lawrence Traa who introduced himself this way.
"I'm the luckiest man in the world."
The 52-year-old former general manager of Standard Areo was pulling on a shirt backstage at the rehearsal the day before when he startled me with those words. This was after he told me the message his doctor had given him last year just before Christmas.
That he had a brain tumour and maybe two years to live. There was more bad luck where that came from.
Less than five years after he had to tell his then-teenage children that their 45-year-old mother's cancer, after being in remission for more than seven years, had spread to her liver. Lorilee Traa had only days to live.
"Someone asked me, 'What's the toughest thing you've ever had to do?' "
Tougher, even, than telling his daughter, Morgan, and son, Mitchell, that he had two years to live because there was nothing that could be done for his brain cancer.
Except that's not how it turned out because Traa wouldn't allow it.
He remembered something and someone that might help him find and receive the advanced treatment he so desperately needed.
The something he remembered was being at a Canadian Manufacturers and Exporters event in March 2011 where an award was presented to IMRIS, a Manitoba- conceived enterprise that the year before had acquired cutting-edge neuroArm imaging technology. The kind that is used for advanced brain surgery.
The someone he remembered was Ed Richmond, a friend and former boss at StandardAero who had been an executive at IMRIS.
Traa contacted Richmond and Richmond contacted Dr. Garnette Sutherland, a professor of neurosciences at the University of Calgary's Foothills Medical Centre and the father of the technology that, at that time, Winnipeg didn't have.
Dr. Sutherland agreed to review Traa's file, and told Traa to come to Calgary.
They could operate.
Last March, Traa underwent a 14-hour operation during which a team led by Sutherland removed 70 per cent of the tumour.
Traa said suddenly his life expectancy went from two years to eight. That's why Traa calls himself the luckiest man in the world.
But we're in luck, too.
The technology that had prolonged Traa's life is here now.
In mid-September, Health Sciences Centre announced the opening of its $25-million centre for surgical innovation; one of only seven in the world.
But there's more good luck where that came from. One day recently Traa was at CancerCare Manitoba having his blood monitored when he saw a young woman who was weeping. He asked if he could help in some way. The 39-year-old mother of three told Lawrence she had brain cancer, and she was waiting to be the first patient to have brain surgery in the new centre for surgical innovation and she didn't know what to expect.
And there was Traa to help her get through it.
Just as Traa and my other new cancer-survivor friends helped me get through the fear of that dreaded fashion show.
By walking that walk together.


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Department of Veterinary Medicine
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text:
Get the Recipe
If you've ever found yourself thinking "this pecan pie simply isn't sweet enough", here's a seasonal variation which is bound to make your dentist cringe: Candy Corn Pecan Pie.
Listen, I wish I could take credit for this idea. But it was the brilliant Rachel of Coconut & Lime who planted the seed in my head and who also (thank you!) gave her blessing for me to make this monstrous bit of Halloween magic happen.
The idea is simple: classic pecan pie, plus candy corn. The candy corn melts so beautifully into the corn syrup and brown sugar mixture that it must feel like it's coming home; it adds a nice, vanilla-y sweetness to the pie that will satisfy even the sweetest of tooths. In fact, it's so sweet that you may push your candy sack aside after a slice. So you see, it's the perfect food to enforce moderation!
Happy Halloween!
About the author: Jessie Oleson Moore is a writer, illustrator, gallery owner, and cake anthropologist who runs Cakespy, an award-winning dessert website. Her first book came out in October 2011, and her second book came out in May 2013.


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All Android applications categories
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Chapter 4:Snoring prevention
Chapter 5:Snoring remedy
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Chapter 9:snoring surgery
Chapter 10:Anti snoring device
Chapter 11:Ways to stop snoring
Chapter 12:Natural snoring remedy
How To Stop Snoring Tonight guide might be the the solution that you have been looking for for a good night slumber.
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Robert Arnold is an Emmy award-winning investigative journalist who joined the KPRC 2 Investigates team in July 2000.
Arnold's diligent investigations earned him the honor of being named "Best Reporter" in Texas four times -- twice by the Texas Associated Press Broadcasters, once by the Headliners Foundation of Texas and once by the Houston Press Club.
Arnold's work covers a range of issues from exposing government waste and corruption to the impact border violence has on Houston. This work has prompted changes in the law and policy both locally and nationally.
Arnold's continuous work into the links between drug violence along the border and Houston earned him one of three Emmy awards. Arnold's other Emmy awards were earned for work that exposed waste and mismanagement in the Houston Fire Department and the less-than-stellar work ethic of a veteran city official.
The State Bar of Texas has also given two awards to Arnold for work that examined loopholes in safety regulations in the construction industry and the policies surrounding how police executed search warrants based on information from confidential informants.
On a national level, Arnold's long-term investigation into the treatment of those suffering from mental illness earned him the Mental Health America's National Media Award. The National Mothers Against Drunk Driving organization also recognized Arnold for work that exposed how a legal loophole allowed suspected drunken drivers to avoid criminal charges by not having their blood drawn after serious accidents.
Arnold graduated from the University of Houston and began his career as a radio reporter for KNUZ/KQUE and then KTRH before joining Local 2. In that time, Arnold has tackled issues involving the Texas Lottery Commission's selling of scratch-off tickets after top prizes were awarded, the treatment of the mentally and physically disabled living in state schools, the licensing and drug testing of commercial truck drivers, the state's hiring practices regarding employees of nursing homes, human trafficking and the security weaknesses surrounding some of our country's most sensitive pieces of infrastructure.
Arnold has also been dispatched to cover events with national and international implications such as the Sept. 11 attack on the World Trade Center and the BP oil spill. During his career, Arnold has also covered nearly two dozen hurricanes and tropical storms along the Gulf Coast and in Mexico.
In his spare time, Arnold enjoys spending time with his wife, Jennifer, and their "children" -- Gracie, Molly and Fritzi. Arnold is also a movie buff, loves reading (horror is his favorite genre) and playing video games (guilty pleasure).
Arnold is also a long time member of Investigative Reporters and Editors, Inc.
If you would like to e-mail Robert, drop him a line at firstname.lastname@example.com and "like" his Facebook page or follow him on Twitter.
Copyright 2011 by Click2Houston.com. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


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A CHILD welfare officer and youth football coach from Oldbury has been jailed for goading children into performing sexual acts on webcams as he chatted to them online.
Kevin Eaton, who coached children at a Sandwell club, set-up social media accounts under the guise of a schoolgirl and persuaded youngsters to strip for him.
As the 31-year-old started a six-year prison sentence yesterday, the former secretary of the club, which cannot be named for legal reasons, said police checks and safeguarding courses "were not worth the paper they were written on" and called for changes to the system.
The case is likely to send shockwaves throughout the junior sporting world as the club had conformed with all safeguarding rules and regulations.
The club's chairman said the severity of Eaton's offending coincided with his elevation to welfare officer.
After starting as a helper at the club 11 years ago, Eaton qualified as a coach, passed his CRB - Criminal Records Bureau check – and took safeguarding and child welfare courses.
The chairman said the club was "devastated" when Eaton was arrested and he was immediately dismissed.
Wolverhampton Crown Court heard Eaton, of no fixed address, was caught after the mother of a 13-year-old girl reported online threats he had made to her daughter.
Detailed IT analysis by West Midlands Police officers traced the computer Eaton had used to an address in Aldridge Road, Oldbury.
The force's Child Online Safety Team's examination of Eaton's online activity found he had contacted up to 100 children pretending to be a 15-year-old girl called Charlotte Large, including two boys he coached at the football club.
Eaton was charged with 20 counts of inciting boys and girls to engage in sexual activity in front of webcams, seven counts relating to making indecent images of children having encouraged them to send him naked photos and three of distributing the images.
Nineteen of the incitement charges were against seven children aged between 11 and 14 which officers were able to identify, whilst the final generic charge related to 23 young victims police have not traced.
He admitted all 30 offences committed from July 2011 to March 2013 and has been ordered to sign the sex offender's register for life.
A sex offender's prevention order was also passed preventing Eaton from ever working with children including in a voluntary capacity and from sleeping in the same building as any children under 16-years-old.
Investigating officer, Detective Constable Rob Piper, said: "Many of these children realised they'd gone too far in sending naked images of themselves and were petrified 'Charlotte' would carry out threats to share the images with friends and family if they didn't bow to his demands.
"One of his victims was so upset by Eaton's actions that she tried committing suicide."
He urged parents to be vigilant of their children's online activity and to question whether they should have webcams in their bedrooms.


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A WOMAN was assaulted while out jogging in Evesham this morning.
The victim was jogging along the riverbank between Abbey Bridge and Evesham Rowing Club at about 7.15am when she was approached by a man who grabbed and assaulted her.
She managed to run off and escape the attacker, who is described as white, in his early 20s, clean shaven and was said to be "expressionless" with a thin and gaunt face.
He was wearing a grey hooded top pulled tightly over his face and dark denim jeans and was carrying a fishing rod.
Detective Sergeant Kate Stevens has appealed to anyone who may have been in the area at the time to contact her.
"We are taking this assault very seriously and wish to speak to anyone who may have seen a man of this description in the area this morning," she said.
"We particularly wish to speak to a woman who was walking a Jack Russell dog prior to the incident who may have vital information to share."
Anyone with any information can call 101 quoting incident 112 of Monday, September 1.
Information can also be passed anonymously to Crimestoppers on 0800 555 111 or www.crimestoppers-uk.org.


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Dreamweaver CS5 11.0.3 Updater
Windows Link:
Size: 14.42 MB
Macintosh Link:
Size: 14.12 MB
Description: This update addresses workflows established in the Dreamweaver CS5 HTML 5 Pack Update such as Multiscreen Preview, Media Queries, Code Hinting and Live View rendering. It is recommended for all Dreamweaver CS5 users. This update also includes updates previously provided by the Dreamweaver CS5 11.0.2 Updater and BrowserLab/Dreamweaver CS5 integration update.
Notes: Please restart the computer after installing the update.
Release Date: 08/31/10


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The PDF is one of the file types I interact with frequently for work alongside word processing documents, spreadsheets, audio, video and images. This app lets you mark up PDFs via touch or mouse. Let the page bleed with red ink: you can highlight, chicken-scratch and strike-through to your heart's content. On my touchscreen ultrabook I found the experience a bit awkward. It helps to have a light touch when making marks and the interface and responsiveness could use some work. I imagine the experience improves on the Surface Pro or other Windows 8 tablets. Nonetheless, for the price this app was a no-brainer for me to make my touchscreen more usable.
Office 2013: 10 Questions To Ask
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Windows 8 Doubt: 4 Ways To Sway PC People
Microsoft Dangles Windows 8, Office 2013 Bundle
SkyKick Tackles Microsoft Office 365 Migration Headaches
SMBs Love Mobile, Struggle On Strategy
Tell Me Again: Why Rush Into Windows 8?


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Where can you find the edition of The Old Farmer's Almanac that's best for you?
Where Each Edition Is Available
The National, or East Coast, version, as well as the Southern and Western editions, are available in those regions on newsstands and in book, hardware, and grocery stores. The Old Farmer's Almanac is also available in our online store.
We also publish a Canadian Edition of the classic Old Farmer's Almanac. It is available in Canada on newsstands and in book, hardware, and grocery stores or in our online store.
We do not publish a traditional large-print edition. However, our black-and-white Hardcover Collector's Edition offers slightly larger pages than our paperback edition, whiter paper, and high-quality print, making the text and images crisper and easier to read.
For the modern age, we also offer a Digital Edition of The Old Farmer's Almanac. This online version not only allows you to read the complete contents of the Almanac but also lets you e-mail, share, print, and search by keyword.
All of our products are available in our online store.


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Muschamp paying no attention to stats
Published: Friday, October 11, 2013 at 6:50 p.m.
Last Modified: Friday, October 11, 2013 at 6:50 p.m.
BATON ROUGE, La. — Back in his defensive coordinator days, Will Muschamp obsessed over statistics. He would judge his defense's performance a success or failure based on the numbers on the stat sheet.
Who: No. 17 Florida (4-1) at No. 10 LSU (5-1)
When: 3:30 p.m. Saturday
Radio: WRUF
Well, those day are gone.
Now that Muschamp is a head coach, he doesn't care so much about the numbers anymore. He's more into letters. As in the "W" or "L" that appear in the win/loss column after each game.
"As long as we win, I don't care," Muschamp said. "Honestly. I got real caught up in all that stuff when I was a coordinator. When I'm a head coach, I want to win. If it takes 51-50, let's go.
"I used to grab those stat sheets when I was a coordinator. Not anymore. There's one stat that matters, and that's winning. Do what you've got to do to win game.
"It's hard enough on Saturday afternoon without worrying about all that other stuff. Just do what you've got to do to win."
The winning vs. the statistics thing came up with Muschamp this week because many are wondering what kind of numbers LSU's potent offense might be able to put up against Muschamp's defense today in Death Valley.
It is something to consider. As good and as solid as the Gator defense has been (UF leads the SEC in eight categories on the stat sheet, including scoring defense and total defense), it has not been tested the way it figures to get tested today.
Led by quarterback Zach Mettenberger, tailback Jeremy Hill and wide receivers Jarvis Landry and Odell Beckham Jr., the LSU offense has been flying up and down the field (and into the end zone) at a record pace this season.
They're hotter than Cayenne pepper.
The Tigers are averaging 45.5 points and 488.8 yards a game. They put 59 points on Mississippi State a week ago, 41 on Georgia the week before. For the first time in school history, LSU has gone for more than 400 yards and scored 30 points or more in six consecutive games to open the season.
Mettenberger is on pace to break the school records for passing yards and touchdowns. Hill is second in the SEC in rushing (118.8 yards a game) and leads the league in rushing touchdowns (nine). Landry and Beckham have combined for 77 receptions for 1,302 yards and 13 touchdowns.
The numbers are staggering. But Muschamp isn't into the numbers.
Even though he's a prideful defensive guy, Muschamp said he'd be fine with the Gators giving up 50 points today as long as they score one more than that.
"Absolutely," he said. "And then we'd watch the film on Sunday and figure out what we need to do to get better."
But could the emotional Muschamp physically endure a 51-50 game?
Muschamp laughed and said, "Winning is all that matters."
LSU's offense has shown it is capable of scoring 50 points (or more). But can the Tigers do it against the best defense, statistically, in the SEC?
Mettenberger said it's a big challenge.
"I think Florida has a combination of both athleticism and great schemes," Mettenberger said. "They have a great defensive coordinator, but they also have tremendous athleticism on the defensive side of the ball. They have big guys that can run, linebackers that can cover and corners that cover very well.
"We will have our opportunities to be man-on-man with the defense and hopefully we can score more times than not against them."
The UF defensive players promise this will be the toughest test of the season so far for Mettenberger and the LSU offense.
"I don't think anybody in the country is playing better defense than we are," junior defensive tackle Darious Cummings said. "We haven't even reached our max yet. We have that relentless effort and we're not taking no for an answer. We get challenged week in and week out to be the best."
Cummings said he doesn't expect today's game to get away from the defense and turn into an offensive shootout.
"Oh, not at all. No sir," he said. "We're not going to allow ourselves to let that happen. Coach Muschamp is a defensive coach and a great mind, and he's going to adjust and we're going to get it done.
"We're going to go out there and do our jobs. We're going to try and stop them the best we can."
Outside linebacker Ronald Powell said the goal of the defense is not necessarily to hold the Tigers to a certain number or yards or points.
"Our goal is to just come out and play Gator football," he said. "We're not going to roll the hat out (and not play hard).
"We're going to come out there as juiced up as we can, come out and play our football, play smart football and let the plays come to us."
If that strategy fails, the Gators might find themselves having to score a bunch of points to keep up, something the offense has not shown an ability to do this season.
The Gators are 13th in the SEC in scoring offense (25.0 points a game). Back to those statistics again.
"I think we're capable of (keeping up in a shootout)," quarterback Tyler Murphy said. "But with the defense we have, I think it's just as important that we take care of the ball, try to stay on the field as much as possible.
"If we do that, if we keep their offense off the field, I think we can be successful."
Contact Robbie Andreu at 352-374-5022 or email@example.com. Also check out Andreu's blog at Gatorsports.com.
Reader comments posted to this article may be published in our print edition. All rights reserved. This copyrighted material may not be re-published without permission. Links are encouraged.


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by Joseph Smith Sr.
We have finally made contact with our old friend Jose Santos. He has allowed us to go into the archives and re-publish some of his works that was published years ago. He also assured us that he would produce some new "All-In" cartoons to add to the mix so stop by weekly and see how poker registers through his tequila infused eyes.


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CHILDREN'S mental health services in south Cumbria were found to be 'inadequate' following the death of Kendal schoolgirl Helena Farrell.
A Serious Untoward Incident Report was carried out into the Child and Adolescent Mental Health Service (CAMHS) after the 15-year-old was found hanged in Kendal woodland in January, 2013.
It found five areas the service needed to urgently improve, including a failure to record the level of risk Helena posed to herself, issues with the level of competency of some CAMHS staff and information sharing, which was judged to be 'inadequate'.
Lynette Moore, clinical services manager at the Cumbria NHS Partnership trust, said the the 'purpose' of the review was to 'learn lessons'.
"It's to change procedure in a timely way so that anything that urgently needs to be addressed can be done so."
The evidence was heard at the second day of the inquest.
The hearing opened yesterday with claims from Helena's father, Enda Farrell, that the service at the time was 'in disarray'.
The court heard that although Helena had sought help on December 3 she wasn't referred to CAMHS until December 10.
She was referred after telling school nurse, Donna Moore, that she had been sexually assaulted on an exchange trip to Germany and that this had led to 18 months of bulimia.
On the evening of December 3 she took an overdose of paracetamol and at a party later that month attempted to cut herself with a knife.
The court also heard she was 'heartbroken' after breaking up with former boyfriend, Billy Williams.
The Kirkbie Kendal School pupil was found hanged in woodland behind the town's Castle Green Hotel on January 4.
She was found at around 6.50pm by Mr Williams, now 18, who had been searching the area for her with a friend of his, his mother and Cumbria policeman, PC Paul Kelly.
"I wasn't expecting it," said PC Kelly. "I wasn't expecting to see what I found."
Helena previously attended the Windermere School and was a high achiever in both sports and the arts.
"My daughter to me was a very bright, intelligent and adventurous, fun-loving, life-loving girl," added Mr Farrell.


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MOTORISTS are facing an autumn of delays, disruption and diversions on the A64 in North Yorkshire because of a £2.2 million resurfacing programme.
Several sections of the route between Bramham and Malton will be affected by the Highways Agency project over a two month period, starting on September 1.
An agency spokesman said: "Work will be carried out in five phases and will improve 8.5 miles of this North Yorkshire road.
"The work includes carriageway resurfacing and patching, the renewal of road markings and road studs, re-instating traffic monitoring loops and renewing damaged road gullies."
Project manager Steven Wright said the scheme would prolong the life of the carriageway, and had been designed to improve the surface and boost safety for road users, while minimising the need for future work.
From the beginning of September until October 2, work will take place on Monday to Thursday nights, between 8pm and 6am. From October 6, until the works have been completed, work will take place every night between 8pm and 6am.
Phase 1, between Bramham and Headley Bar, will mean overnight eastbound closures from September 1 to 11.
Phase 2, between Flaxton Moor and Barton Hill Crossroads on the eastbound carriageway, will cause lane closures with temporary traffic signals and convoy working from September 15 to 23.
Phase 3, between Grimston Bar and Hopgrove Roundabout, will mean overnight eastbound carriageway closures from September 24 to October 9.
Phase 4, between Tadcaster and Askham Bryan, will mean overnight eastbound closures from October 10 to 25.
Phase 5, between Bilbrough Top and Tadcaster, will mean overnight westbound closures from October 26 to November 3.


text:
The distributor may develop internal faults. This can cause a rough running engine or stalling condition, the Check Engine Light may or may not illuminate. Our technicians tell us that the distributor should be overhauled or replaced to correct this concern.
Problem Description and Possible Solution
Problem Data
RepairPal Verified
Average mileage: 192,000 (192,000–192,000)
Engine affected: 4.2L 6 Cylinder
7 people reported this problem


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A Valentine's Day good deed will be carried out tomorrow to help a 93-year-old Otley man.
The area's Neighbourhood Policing Team (NPT) had originally been called in to investigate the chopping down of trees at the back of Desmond Fox's house on Cambridge Drive.
The removal of the trees left the pensioner's garden exposed and caused his shed, which had been resting against them, to collapse.
The police decided the matter was a civil rather than a criminal one, so did not take the investigation further, but were determined to lend a helping hand to Mr Fox, who uses a disability scooter and is partially deaf.
So they appealed to businesses to help supply materials and, tomorrow, will put up a new fence to make his home feel secure again.
Otley-based NPT member PC Sam Emsley said: "We had a look at the garden and it was quite a mess, with numerous stumps left exposed. It looked like the person who had chopped the trees down had used a chainsaw.
"As a result, Des's garden shed fell down as it was propped up against the trees and he had to pay someone £100 to come and dismantle it. Des did seek some advice from a solicitor, but they told him he would have to have the fence repaired or replaced himself.
"When we spoke to him he was very distressed and did not know where to turn to sort it out. I decided to see if I could get the local businesses to help by donating the materials to replace the fence and put a new one up to make his garden secure."
B&TS Builders Merchants in Otley responded by donating four fence panels while Homebase, at Moor Allerton, Leeds, supplied five fence posts and post mix.
Aireborough & Wharfedale NPT Inspector Richard Coldwell praised PC Emsley and her colleagues.
He said: "They are doing a really wonderful thing for a deserving elderly man, with the support of some local businesses who also deserve a big mention."


text: Public Notice Notice of Sheriff's Sale By virtue of a certified copy of a decree to me directed from the Clerk of the Superior Court of Jay County, Indiana, in Cause No. 38D01-1206-MF-000032, wherein JP Morgan Chase Bank, N.A, was Plaintiff, and Sean Torre a/k/a Sean Daniel Torre, was a Defendant, requiring me to make the sum as provided for in said Decree with interest and costs, I will expose at public sale to the highest bidder, on the 5th day of December ,2013, at 10: 00 A.M. of said day, at the 3rd Floor, Courthouse, Portland, IN 47371, the fee simple of the whole body of Real Estate in Jay County, Indiana: Lot numbered Fifteen(15) in Hugh's Second Addition being an addition in a part of the East Half of the Northeast Quarter of Section 19, Township 23 North, Range 14 East, Wayne Township, now Portland, Indiana. 38-07-19-101-020.000-034 and commonly known as: 112 Silvers Lane, Portland, IN 47371. Subject to all easements and restrictions of record not otherwise extinguished in the proceedings known as Cause # 38D01-1206-MF-000032 in the Superior Court of the County of Jay Indiana, and subject to all real estate taxes, and assessments' currently due, delinquent or which are to become a lien. Said sale will be made without relief from valuation or appraisement laws. If the sale is set aside for any reason, the Purchaser at the sale shall be entitled only to a return of the deposit paid. The Purchaser shall have no further recourse against the Mortgagor, the Mortgagee, or the Mortgagee's Attorney. Larry R. Newton Jr. Jay County Sheriff James L. Shoemaker (19562-49) S. Brent Potter (10900-49) Craig D. Doyle (4783-49) Curt D. Hochbein (29284-29) Ryan T. Kiernan (29316-49) David M. Johnson (30354-45) Evgeny G. Mogilevsky (27602-49) Tina M. Caylor (30994-49) Doyle Legal Corporation, P.C. 41 E Washington St., Suite 400 Indianapolis, IN 46204 Telephone (317) 264-5000 Facsimile (317) 264-5400 CR 11-4,11,18-2013 - HSPAXLP


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Hey there, time traveller!
This article was published 16/10/2012 (1771 days ago), so information in it may no longer be current.
A look at the other names, aside from QBs, on the Bomber neg list, obtained by The Free Press on Monday:
Mario Addison DE Troy
Undrafted in 2011, signed by the Bears, then to the Colts, now with the Redskins. Has played end and now linebacker.
Terrence Austin WR UCLA
Seventh-round pick of the Redskins in 2010, he was one of the club's last cuts this season and is now a free agent. Played in 18 games over two years with Washington and pulled in 15 passes.
Josh Bellamy WR Louisville
On the Kansas City Chiefs practice roster after standing out as undrafted player at their rookie mini-camp. Ran a 4.47 and played some corner as well at Louisville.
Claude Davis DE South Florida
Wasn't drafted and was injured around the time NFL teams expressed an interest. Signed earlier this month with the Bay Area Pirates of something called the United Football Federation.
Desia Dunn DB Fresno State
Had a stint with Arizona Cardinals and then in the Arena League.
Pat Edwards WR Houston
Currently on the Detroit Lions' practice squad; was the Conference USA Offensive Player of the Year in 2011.
Dequin Evans DL Kentucky
On the Cincinnati Bengals practice squad and former starter with the Wildcats.
LaQuinton Evans WR Southern
Cut by the Tennessee Titans in August. Just FYI: very cool nickname — 'Smoke.'
Jeff Fuller WR Texas A&M
Now with the Miami Dolphins on their PR; Big target (6-4) set school record for career TD receptions.
Tyrell Gaddies DL Louisiana at Lafayette
Former Rajin' Cajuns star is a defensive tackle listed at 6-2, 275.
Darryl Gamble DE Georgia
Undrafted out of Georgia, Gamble first signed with San Diego and is now on Washington's practice roster.
Greg Gatson DB Arkansas
Small by NFL standards at 5-11, 165, Gatson is on the San Diego practice roster.
Logan Harrell DL Fresno State
Defensive end was a 2010 All-WAC Allstar. Another member of the Chargers PR squad.
Kareem Huggins RB Hofstra
Cut by New England in August. Spent two years with the Tampa Bay. Rushed for 2,188 yards and 21 touchdowns at Hofstra.
LeQuan Lewis DB Arizona State
Cornerback; was just released by Dallas after being snatched off the New York Jets' practice squad.
Eric Norwood DE South Carolina
Former first-team All-American in 2009 after setting school records in sacks and tackles for losses. Was cut by Carolina in August.
Ty Nsekhe OT Texas State
Human eclipse of a man at 6-8, 325 who is on the St. Louis Rams practice squad.
Louis Nzegwu DE Wisconsin
Added to the Dolphins PR last week after being dumped by Atlanta.
Junior Onyeali DE Arizona State
A junior with the Sun Devils who starts at defensive end.
Dj Porter DB Stony Brook
Led Big South conference in interceptions last year.
Armond Smith RB Union College
Undrafted free agent first signed by Cleveland, now on the Carolina practice roster.
Corey Smith LB Cincinnati
Starred with the Bearcats.
Deonte Thompson WR Florida
Former Florida Gator is on the Baltimore Ravens active roster.
Ross Weaver CB Michigan State
Has had stints wit Miami, Seattle, Dallas and the Jacksonville Sharks (indoor) and was released from Detroit's practice squad in September.
Martez Wilson LB Illinois
On the active roster of the New Orleans Saints; drafted in the third round of 2011.
Note: the Oct. 8 Bomber neg list included Jawann Westerman, Vincent Agnew, R.J. Archer, James Carmon and Wallace Miles, all currently on the Bomber practice roster, as well as Lee Barbiasz, who reported to Winnipeg but left a day later.


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Gluten-Free Girl's Irish Soda Breadby Healthy Eats in Gluten-Free, March 14, 2011
Shauna James Ahern, better known as Gluten-Free Girl, is sharing her favorite gluten-free recipes with us! This week, an Irish classic: Soda bread.
St. Patrick's Day is coming up. Everyone will be Irish for the day, dressed in green and hoping to not get pinched.
Most people think of corned beef and cabbage when they think of St. Patrick's Day foods. It's nice, no question. However, when I was in Ireland on St. Patrick's Day, 10 years ago, I was shocked to find that no one ate corned beef and cabbage on that celebration day. No one.
Brown soda bread? That's eaten all through the year and on St. Patrick's Day, too.
Soda bread takes only a few moments to make. It requires no yeast, so no worrying that your yeast is old or your kitchen too hot. You simply throw some flours in a bowl, add rolled oats, baking soda and salt and stir in some buttermilk. Slide it into a hot oven and you have bread in under an hour. Crusty, warm bread.
And gluten-free? The crust is just as crackly, the crumb just as soft, the slices just as firm, and the bread just as delicious as the gluten version.
Gluten-Free Irish Soda Bread
Adapted from Colman Andrews' The Country Cooking of Ireland
Butter for greasing
Sweet rice flour for flouring
150 grams (2/3 cup) almond flour
150 grams (3/4 cup) sorghum flour
100 grams (1/2 cup) sweet rice flour
50 grams (1/3 cup) teff flour
50 grams (1/4 cup) potato flour
10 grams (2 tablespoons) flax seed meal
80 grams rolled oats
2 teaspoons baking soda
1 teaspoon kosher salt
1/2 cup currants
2 to 4 cups buttermilk
1 large egg plus 1 tablespoon water, mixed
Preheat the oven to 375.
Grease a baking sheet with butter, then dust it with sweet rice flour.
Mix together the almond flour, sorghum flour, sweet rice flour, teff flour and potato flour. Whisk them together to aerate and incorporate them. Mix in the flaxseed, oats, baking soda and salt. Toss in the currants and mix them into the flours.
Make a well in the center of the dry ingredients and pour in the buttermilk. Add buttermilk, mixing, until the dough is moist and soft but not wet, with no visible flour left. You will probably use about 3 cups, but feel free to use less or more, depending on your kitchen.
Turn the dough out onto the baking sheet. With lightly floured hands, shape the dough into a round about 3 inches thick. Cut a deep cross into the top of the loaf with a wet, serrated knife. Brush on the egg wash evenly.
Bake until the crust is dark brown and you hear a hollow thump on the bottom when you tap it, about 45 to 60 minutes.
Happy St. Patrick's Day!
Shauna James Ahern blogs about living the (gluten-free) sweet life at Gluten-Free Girl and the Chef. Her new cookbook, Gluten-Free Girl and the Chef, is available in bookstores now.


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The City of Evansville, Indiana, was founded in 1812 on a scenic bend in the Ohio River. With a population of nearly 121,000 people in the city limits, and more than 300,000 people in the metropolitan area, Evansville is the third-largest city in Indiana. Evansville is the social and economic hub for our region, which includes Southwest Indiana, Southeast Illinois and Northwest Kentucky.
Interesting facts about Evansville include:
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Punch out the puzzle pieces, then match the numbers. Follow the detailed instructions to bring to the Robocrocodile to life! For ages 8+ Exclusively available for purchase online here at Go! Games and Puzzles.


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A BROMSGROVE woman is preparing to put her best foot forward when she takes part in the London marathon.
Lara Jackson, a team leader at Altered Images, is participating in the marathon this Sunday, April 13.
She has run the 26-mile challenge before but said she was excited to again have the chance to run it.
The Worcester Road-based gym has a partnership with the Stroke Association and Lara said she had been inspired to run after hearing the stories of stroke survivors.
One of the survivors is Pete Rumbold, a lifelong member of the gym who had a stroke in 2012. Since the stroke he has been training at the gym to aid his rehabilitation.
He has also recently qualified as an Action for Rehabilitation of Neurologicial Injury (ARNI) personal trainer, a qualification that Lara has also been working towards as the gym are planning to have a new ARNI facility.
Lara said she was looking to raise £1,000, but generally wanted to raise as much as possible.
"I have seen how far Pete has come, which has inspired me to run for the Stroke Association," she said.
"All the money I raise will go towards helping stroke survivors from Bromsgrove, particularly as the charity's headquarters are in the town."
The club runner said she was already able to run half marathon distances but had stepped it up with a tough 12-week schedule, so that she will be able to run the full distance on Sunday.
Anyone who wants to make a donation is asked to visit Lara's fundraising website, justgiving.com/altered-images-gym.


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Scandi Home has finally found a new home. A few more tweaks are still needed, but you can have a sneak peek at
This blog will remain as is and any recipes I am posting will still be shared on this website. Interior related posts will go on the new scandihome.com.au website. Hope you like it!


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Some shots of our table at the Canberra fair…
Tim successfully avoids being photographed, just after set up in Gorman house.
Later, some of the gallery staff came and took away a number of the tables, which they had apparently filched from somewhere without permission and had to return. The empty table next to us was reserved for Bird in the Hand distro, who did not, unfortunately, materialise. Presumably Susy learned that she'd be seated next to us and, aware of our legendary pugnacity (or perhaps pungency), decided against it.
In case you were wondering what goes on behind the table at a zine fair (legs and boxes/ bags of crap).
Later in the afternoon, Tim discovered that Emma's scarf could be fashioned into a something not unlike an amusing, woollen breast.
Which, with slight alteration, could be turned into a serviceable, almost Taitlinesque, new hat for Emma. Note bags under eyes, result of waking up at 5 in the morning to get to Canberra in time for zine fair. The things we do for you, honestly.
We left shortly after this photo was taken, for reasons that are probably obvious.
Thanks again to everyone who came!


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Nirav P. Patel, M.D., M.P.H., F.C.C.P., F.A.A.S.M., born in Nadiad, India, raised in London, England, and resident of Wyomissing, Pennsylvania, and physician specializing in sleep disorders, pulmonary medicine and critical care, passed away suddenly on May 17, 2012, of a myocardial infarction from coronary atherosclerosis. He was 37 years old. Dr. Patel's career was devoted to helping patients with sleep and pulmonary disorders. He was recognized as not only an outstanding clinician, but also as a superb teacher and researcher.
Dr. Nirav P. Patel
Dr. Patel dedicated his research career to advancing our understanding and awareness of the public health impact of sleep disorders. He was especially interested in poverty's negative effects on sleep. He believed that sleep was a link between social inequality and adverse health outcomes. His research examined insufficient sleep attainment by people in disadvantaged groups (such as minorities and the poor)—a situation he called "sleep disparity"—and the reasons why these groups often have worse long-term health outcomes. He worked tirelessly to help increase awareness of the negative effects of inadequate sleep, such as increased risk of diabetes, motor vehicle accidents, and cardiovascular disease, through participation in community health fairs, his work as a board member of the Pennsylvania Sleep Society, and his numerous research publications. In 2008, he organized a "Population Sleep" conference at the University of Pennsylvania, which was attended by over a hundred participants from across the nation. He was instrumental in adding sleep questions to the Public Health Management Corporation (PHMC) annual survey, a key regional assessment of public health in the Philadelphia area. Despite his brief career, he has published extensively: His research, consisting of 36 manuscripts and 26 abstracts, has been published in leading journals in the sleep and pulmonary medicine communities, such as the journal SLEEP, and has been presented at international conferences.
Dr. Patel began his medical education at Guys, Kings – St Thomas University Medical School, King's College in London, United Kingdom. He moved to the Philadelphia area in 2001, where he completed his internal medicine training at Albert Einstein Medical Center. He then came to the University of Pennsylvania for a fellowship in pulmonary medicine, critical care and sleep medicine. He joined the faculty of the Division of Sleep Medicine in 2008 where he was an Assistant Professor of Medicine. He also completed a Masters of Public Health at the University of Pennsylvania in 2009. Throughout his training and subsequent career, he loved caring for and learning about complex medical conditions. This passion led to numerous awards, including the Howard Rogers Prize for Medicine and the Exemplary House Physician award from University Hospital Lewisham (United Kingdom). Dr. Patel was also a member of the 2006 winning team for the nationwide American College of Chest Physicians "Chest Challenge" competition.
Dr. Patel left the University of Pennsylvania in 2009 to take a position at Respiratory Specialists in Wyomissing, Pennsylvania, where he also attended at The Reading Hospital Medical Center and St. Josephs Medical Center. He quickly developed a reputation as a kind, committed and hard-working physician who received awards for outstanding physician house staff teaching and showed that his love of learning extended to a desire to share that knowledge with others. Despite his significant clinical workload, he continued to be actively involved in public health, pulmonary and sleep medicine research activities, which recently resulted in his being considered for Adjunct Assistant Professor of Medicine at the University of Pennsylvania.
Those of us who knew Dr. Patel remember his vitality, commitment and incredible perseverance. In 2006, he was a passenger in a severe motor vehicle accident that resulted in debilitating elbow injuries which ultimately required five separate operations and left him with chronic pain and reduced arm mobility. Despite this traumatic, near-death experience, he maintained his optimism, love of life and family, and commitment to patient care and research. We are fortunate to have had his star burn so brightly in our presence even for this short time.
His philosophy in life was to never to put off till tomorrow what you can do today. His interests included connecting with people, traveling, and sports. His passion, however, was being there and helping people. Dr. Patel is survived by his wife, Minal, son Ariyan (age 5), daughter Rhea (age 3), and by his parents and sister, of London, England.


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After teasing users since April 2011 with previews, Microsoft has at long last released a complete version of Internet Explorer 10 for Windows 7. While the company promises that the browser loads "real world" Web pages up to 20 percent faster, developers are likely more interested in the broader Web-standards support, particularly HTML5 -- assuming they've exercised enough patience for IE10 to come to Windows 7.
In a blog post, Microsoft's Rob Mauceri, group program manager for Internet Explorer, announced the availability of IE10 for Windows 7 and detailed the browser's superiority over IE9. His post received mixed responses from the MSDN community, ranging from "Hurray!" to "Why the heck did this take so long?"
Back in 2011, Microsoft unveiled its first preview version of Internet Explorer 10, which ran on Windows 7 and later. The second preview also run on Windows 7, but all that followed were Windows 8-only until last November. IE10 for Windows 8, meanwhile, reached general availability last September.
IE10 for Windows 7 adds support for 30 new Web standards, including the latest HTML5, CSS3, DOM (Document Object Model), Web Performance, and Web Application specifications, wrote Rob Mauceri, group program manager for Internet Explorer. Support for CSS Text Shadow, 3D Transforms, Transitions, and Animations enable developers to create rich visual effects, according to Mauceri. Developers also can create more sophisticated and responsive page layouts with CSS3 and HTML5 forms, enabling UI tricks like flexbox, positioned floats, and regions.
Support for local storage via IndexedDB and the HTML5 Application Cache will help developers create better offline applications, he said. IE10 also supports HTML5 Drag-drop, File Reader API, Media Query Listeners, and Pointer Events, which can aid in building more interactive Web apps.
On the security front, Mauceri said that IE10 offers superior Web-app security with the same markup and support for HTML5 Sandbox for iframe isolation.
There are differences between IE10 for Windows 7 and for Windows 8, all of which involve touch-related APIs. For example, IE10 on Windows 7 will not fire certain DOM events, such as MSPointerHover, MSPointerMove, MSGestureStart, MSGestureTap, and MSManipulationStateChanged. IE10 for Windows 7 also doesn't support certain CSS scrolling, zooming, and touch properties, as well as aria-haspopup, a DOM property supported on Windows 8 that simulates mouse hover functionality in touch-based scenarios.
Developers can use Microsoft's recently launched modern.IE tool to test and verify their sites. It offers providers a wizard that scans a Web page URL for common interoperability problems and suggests some ideas for how to address those issues.


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1923-1926 — Tipp City, Ohio
Sometime between 1923 and 1926 Les Eickhoff and A. E. "Bake" Staup became co-owners of this barbershop on the south side of East Main Street between First and Second Streets in Tipp City. He was still barbering here when he had his fatal heart attack. Betty Barnhart Eickhoff also worked here during the month that they sold driver's licenses and automobile tags. In those days everyone got their license at the same time of year. In the back room of the building was a big steam press where Don Barnhart, Betty's brother, worked pressing clothes for customers.


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Looking for more information about Joseph Shirk?
Search through millions of records to find out more.
Search through millions of records to find out more.
1779-1781 — Franklin County, Pennsylvania
American Revolutionary Soldiers of Franklin County, Pennsylvania, compiled by Virginia Shannon Fendrick for the Franklin County Chapter, Daughters of the American Revolution, Chambersburg, Penna., published by Historical Works Committee of the Franklin County Chapter, 1944
p 194-195
Served as private under Capts. John Rea and John Reed, 1779-80-81. He was of Green Twp., but owned land in Letterkenny. He names 11 children: Casper, Fredk; Joseph; Christly; Abraham; Jacob; Peter; Elizabeth mar. John Long; Barbara mar. Jacob Houser; Christina mar. John Leaman; Magdalena mar. Geo. Sellers; grandson Samuel of son John. This will was probated May 22 1811.
-Penna. Arch. 5th Ser. Vol. 6, p 78, 103, 544, 585
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You exercise your body to stay physically in shape, so why shouldn't you exercise your brain to stay mentally fit? With these daily exercises you will learn how to flex your mind, improve your creativity and boost your memory. As with any exercise, repetition is necessary for you to see improvement, so pick your favorite exercises from our daily suggestions and repeat them as desired. Try to do some mentalrobics every single day!
noun :: The fit, attack, or exacerbation, of a disease that occurs at intervals, or has decided remissions or intermissions.
noun :: Any sudden and violent emotion; spasmodic passion or action; a convulsion; a fit.
"And as they obeyed, Michael strained backward in a paroxysm of rage, making fierce short jumps to the end of the tether as he snarled and growled with utmost fierceness at the steward." --London, Jack
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Word of the Day : Loquacious
Word of the Day : Veneration
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Word of the Day : Deliquesce
Word of the Day : Prolix


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Bus Maintenance Facilities Strategic Plan
The Authority has identified and assessed the current bus service, the existing bus fleet, the constraints of existing garages, the future facility equipment needs, and any sites for possible new facilities.
Among highlights of the Strategic Plan for Bus Maintenance Facilities are recommendations for upgrade of system maintenance capacity with medium- and long-term construction of north side and south side facilities. For more details, read the Strategic Plan document below, which has been divided into sections for easier download.
Part 1: Summary, Introduction, and Needs Assessment
Part 2: Recommendations for Wellington and Alewife
Part 3: Recommendations for Revere and Malden
Part 4: Recommendations for Watertown and Riverside
Part 5: Summary of Recommended Options, Environmental Considerations, Funding, and Schedule
All PDFs can be viewed with Adobe Acrobat Reader version 4.0 or higher.
A photo of the existing Wellington station (facing South):
A proposed bus facility and parking garage on the site:
Renderings of a proposed bus facility and parking garage on the site:


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Mie, M., Shimizu, S., Takahashi, F. and Kobatake, E.
Notes: The authors developed an in vitro selection system that is based on ribosome display and favors identification of 5´-untranslated regions (UTRs) with high translation efficiencies. A 5´-UTR random library was created in which the 5´-UTRs were upstream of a polyhistidine-tag/Renilla luciferase-coding region. In vitro transcripts from this library were translated in vitro using the Flexi® Rabbit Reticulocyte Lysate System. The authors preferentially selected mRNAs with high translational efficiencies by shortening the translation time and capturing ternary complexes of mRNA, ribosome and nascent proteins. These complexes were captured using MagneHis™ Ni Particles. RNA was extracted from these complexes and used as a template in RT-PCR for the next round of selection. Before and after each round of selection, 9µl of RNA was translated in vitro, and 20µl of translated product was removed every 5 minutes to measure Renilla luciferase activity and monitor translation efficiency. Renilla luciferase was measured using the Renilla Luciferase Assay System. After two rounds of selection, RT-PCR products were cloned into a pUC18 vector, the sequences of the resulting plasmids were confirmed, and 0.5µg of plasmid was translated in vitro using the TNT® T7 Coupled Rabbit Reticulocyte Lysate System to further evaluate translation efficiency. (3963)
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Flexi® Rabbit Reticulocyte Lysate System
MagneHis™ Ni-Particles
Renilla Luciferase Assay System
TnT® T7 Coupled Reticulocyte Lysate System
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Sarrazin, S., Starck, J., Gonnet, C., Doubeikovski, A., Melet, F., Morle, F.
Notes: Authors used the Flexi® Rabbit Reticulocyte Lysate System to in vitro translate Fli-1 mRNA. The lysate was programmed with mRNAs containing mutated sequences to determine the mechanism of translation initiation of two different isoforms. (2210)
McLaughlin, S.H., Conn, S.N., Bulleid, N.J.
Notes: Transcribed cDNA for type X collagen using Promega's T7 RNA Polymerase. Translated this using Flexi® Rabbit Reticulocyte Lysate System in presence of semi-permeabilized HT1080 cells to allow for correct assembly of fibrillar collagens. (0714)
T7 RNA Polymerase
Zhao, L., Helms, J.B., Brunner, J., Wieland, F.T.
Notes: To produce site-specific photolabile ADP-ribosylation factor mutants, the amino acid of interest was mutated to the Amber stop codon in the cDNA. The cDNA was then in vitro transcribed and the resulting RNA translated in vitro with the Flexi® Rabbit Reticulocyte Lysate System supplemented with a suppressor tRNA charged in vitro with the L-4'-(3-trifluoromethyl-3H-diazirin-3-yl)-phenylalanine. The suppressor tRNA was used at 5µM in the reaction. The reaction was performed with labeled methionine to judge the success of the suppression via autoradiography. The photolabile protein was used to crosslink to proteins interacting with ADP-ribosylation factor. (0057)
Story, C.M., Furman, M.H., Ploegh, H.L.
Notes: Inserts in the pSP72 Vector were in vitro transcribed and translated using the Flexi® Rabbit Reticulocyte Lysate System. (0340)
pSP72 Vector
Shields, M. J., Assefi, N., Hodgson, W., Kim, E. J., Ribaudo, R.K.
Notes: The authors co-translated mRNAs for beta 2 microglobulin and MHC class I proteins in Flexi® Rabbit Reticulocyte Lysate System with Canine Pancreatic Microsomal Membranes (CMMs). They generated mRNAs using RiboMAX™ T7 Large Scale RNA Production System. Treated transcription reactions with RQ1 RNase-Free DNase I after transcription to remove template DNA. (0393)
Canine Pancreatic Microsomal Membranes
RiboMAX™ Large Scale RNA Production System-SP6
RiboMAX™ Large Scale RNA Production System-T7
RQ1 RNase-Free DNase
Illidge, C., Kielty, C., Shuttleworth, A.
Notes: The Flexi® Rabbit Reticulocyte Lysate System was used for cell-free translation reaction in the presence of 4 x 105 digitonin-permeabilized HT-1080 fibroblasts. Two types VIII chains were expressed as well as the a1chain of type X collagen. The expressed proteins were assayed by protease digestion and thermal denaturation for their quaternary structure. The in vitro transcripts for the translation reaction were produced with the Riboprobe® System. (0995)
Riboprobe® System-SP6
Riboprobe® System-T3
Riboprobe® System-T7
Denning, G.M., Leidal, K.G., Holst, V.A., Iyer, S.S., Pearson, D.W., Clark, J.R., Nauseef, W.M. and Clark, R.A.
Notes: PolyA+ RNA from HL-60 cells was in vitro translated using the Rabbit Reticulocyte Lysate System in the presence or absence of Canine Microsomal Membranes, then immunoprecipitated and analyzed by SDS-PAGE and fluorography. The calreticulin protein was presumably glycosylated by the membranes. The same results were obtained with in vitro transcripts of the calreticulin. (1617)
Rabbit Reticulocyte Lysate System, Nuclease Treated
Falk, M.M., Buehler, L.K., Kumar, N.M. and Gilula, N.B.
Notes: Several different gap junction channel subunit isotypes, also known as connexins, were synthesized in vitro in the presence of Canine Microsomal Membranes. Translation reactions were spun through a sucrose cushion to separate the membrane fraction from the supernatant. Connexins that integrated into the microsomes formed homo- and heterooligomeric structures. The assembled proteins in the microsome were fused with a synthetic membrane and single channel conductance was measured and found to be very similar to wild-type membranes. (1618)
Rabbit Reticulocyte Lysate, Untreated
Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System
Gow A., Gragerov A., Gard A., Colman D.R., Lazzarini R.A.
Notes: All cDNAs to be translated in vitro were subcloned into the pSP64 Poly(A) Vector. The vector was linearized and RNA transcribed from the SP6 promoter with the Riboprobe® in vitro Transcription System. The in vitro transcribed RNA was translated in the Rabbit Reticulocyte Lysate in the presence or absence of Canine Pancreatic Microsomal Membranes (CMMs). N-linked glycosylation sequences were engineered into the proteolipid protein to determine the orientation of the domains of the protein in the membrane. (1114)
pSP64 Poly(A) Vector
Roeper, J., Lorra, C., Pongs, O.
Notes: Two site-directed mutants of KV1.4 were expressed in the Flexi® Rabbit Reticulocyte Lysate System in the presence of Canine Pancreatic Microsomal Membranes (CMMs). The membranes were gently pelleted, resuspended and the proteins in vitro phosphorylated with Calmodulin-dependent protein kinase II. (0490)
Wang. S., Krinks, M., Lin, K., Luyten, F.P. and Moos, M., Jr
Notes: To demonstrate an interaction between Frzb and Wnt proteins in vitro, the proteins were cotranslated using Rabbit Reticulocyte Lysate in the presence of Canine Microsomal Membranes then immunoprecipitated with specific antibodies to either one. Control immunoprecipitations were performed with cotranslations with Frzb and the β-lactamase control or Wnt and the β-lactamase control. The procedure for the immunoprecipitation is referenced. (1603)
Gutierrez, J.A., Yu, J., Rivera, S., Wessling-Resnick, M.
Notes: The cRNA for the Stimulator of Fe Transport (SFT) was in vitro translated with Rabbit Reticulocyte Lysate in the presence of Canine Microsomal Membranes. The RRL was used at 70% of total volume and 2 equivalents of the membranes were used. After translation, the reaction was treated with RNase A to reduce background. The SFT protein is predicted to have six transmembrane domains. (1621)
Stumptner, P. and Benaroch, P.
Notes: Reactions were performed at 30°C in a 25µl volume with 3 equivalents of membranes. Following translation, the membranes were lysed with detergent and the proteins immunoprecipitated with various antisera. The immunoprecipitated proteins were also subjected to endoglycosidase H digestion. (1598)
Meera, P., Wallner, M., Song, M. and Toro, L.
Notes: Different truncations of the protein of interest were in vitro translated in the presence of the membranes. The translation reaction were centrifuged and the pellet and supernatants assays for the presence of the protein product. (1628)
Vance, B.A., Wu, W., Ribaudo, R.K., Segal, D.M. and Kearse, K.P.
Notes: The CD69 transcripts were prepared with the T3 RiboMAX® System and translated with the Flexi® System in the presence of the membranes. After 60 minutes at 30°C, the translation reaction was layered onto a sucrose solution and centrifuged at 100,000 x g for 20min. The supernatant was discarded and the pellet was resuspended in SDS-PAGE sample buffer or digested with endoglycosidase H. (1601)
Rangwala, F., Drisdel, R.C., Rakhilin, S., Ko, E., Atluri, P., Harkins, A.B., Fox, A.P., Salman, S.B. and Green, W.N.
Notes: The two products were used to show that the rat a7 cDNA was translated and could be processed in vitro to a higher molecular weight, most likely by glycosylation. (1630)
Hackan, A.S., Wang, T.L., Guggino, W.B. and Cutting, G.R.
Notes: The proteins r1 and r2 as well as the N-terminal half of r1 and the C-terminus of r1 were expressed using the Flexi® Rabbit Reticulocyte Lysate System in the presence of Canine Microsomal Membranes. The r1 protein has four membrane spanning domains. The orientation of the protein was determined by proteinase K digestions of intact membranes. Digestions were terminated by the addition of PMSF rather than direct lysis in SDS sample buffer. (1622)
Gafvelin, G., Sakaguchi, M., Andersson, H. and von Heijne, G.
Notes: The paper looked at the insertion of a model protein with one, two and four transmembrane segments and different distributions of positively charged residues in the N-terminal tail and the polar loops. The proteins were translated in the presence of the microsomes. Translocation of polypeptides to the lumenal side of the microsomes was assayed by prevention of N-linked glycosylation through competitive inhibition by the addition of a glycosylation acceptor tripeptide but not by a nonacceptor tripeptide and by proteinase K treatment of the microsomes. All techniques are referenced. (2033)
Schmidt-Rose, T. and Jentsch, T.J.
Notes: Increasing residues of the chloride channel were fused to a prolactin epitope and translated in vitro using Promega's Rabbit Reticulocyte Lysate in the presence of Canine Microsomal Membranes. The proteins were subjected to a proteinase K digest in the presence or absence of Triton X-100. The protease digestions were stopped with PMSF and the translation reaction analyzed by Western blotting. The protease protection assays were used to define the topology of the channel. The chloride channel crosses the membrane at least 10 times. Very good protocol for the protease protection assay. (1596)
Li.Y., Song, Y.H., Rais, N., Connor, E., Schatz, D., Muir, A. and Maclaren, N.
Notes: The extracellular domain of the receptor was expressed in vitro using Promega's Rabbit Reticulocyte Lysate in the presence or absence of the microsomes. The 60kDa extracellular domain is processed to a 70kDa glycoprotein in the presence of the membranes. The paper also shows a titration of the membranes in the translation reaction from 1-4µl per reaction. More protein is processed with increasing amounts of the microsomes. (1627)
BCIP/NBT Color Development Substrate
Wallner, M., Meera, P. and Toro, L.
Notes: Truncation mutants of the protein of interest were translated in the presence of the membranes. The membranes were pelleted and analyzed directly or treated with endoglycosidase F. The supernatents from the centrifugation were acetone precipitated and analyzed as well. (1602)
Towner, J.S., Ho, T.V. and Semler, B.L.
Notes: The 3AB protein was fused with a FLAG tag. The rabbit lysate was centrifuged prior to use to remove any protein aggregates. The reaction was set up in a 200µl volume with the 3AB protein transcript and a b globin mRNA. The reaction was split into two 100µl reactions and to one 25 equivalents of membranes were added and to the other, just buffer. The translation reactions were allowed to procede for 30min at 30°C, then translation stopped by the addition of cycloheximide to 300µM. The reactions were centrifuged at through a sucrose gradient at 86,000 x g. Fractions were removed and subjected to immunoprecipitation and gel analysis. The 3AB protein was found to be membrane associated. Further studies with the addition of the Canine Microsomal Membranes after translation and cycloheximide demonstrated a membrane association after translation. (1599)
Shtrom, S.S. and Hall, Z.W.
Notes: Translations with the Flexi Rabbit Reticulocyte Lysate System were performed in 25µl volume containing 3 equivalents of membranes. Reactions were performed at 28.5°C and in the presence or absence of the glycosylation inhibitor AcNYT. Translation products were analyzed either directly by SDS-PAGE followed by fluorography or with prior immunoprecipitation of the translation products. Capped transcripts were produced by in vitro transcription with SP6 RNA Polymerase. (2029)
Shayakul, C., Steel, A. and Hediger, M.A.
Notes: The novel UT1 urea transporter was translated in vitro in the presence or absence of the microsomes. In the absence of the microsomes, the protein migrates at 76kDa and in the presence, 86kDa. The data is not shown but described in the text. (1597)