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Chick-Fil-A bully Adam Smith tries to apologize to female Rachel – she refuses to see him – plus video 'apology and clarifications'
Seems Chick-Fil-A bully Adam Smith is getting a taste of his progressive leftist medicine. He went to try and "apologize" to Chick-Fil-A worker 'Rachel' and she refused to see him. The Blaze has an exclusive interview with Adam Smith over how his life has changed over the last 36 years. While I obviously despise what this coward did to the Chick-Fil-A worker, some of the stuff he's dealing with is NOT cool. In fact, it reminds me alot of George Zimmerman situation when Spike Lee tweeted out an address that he thought was Zimmerman's. Seems Adam Smith was forced to leave his home because of death threats to not only him, but also his family. I'm sorry, but that's just not cool. Adam Smith's home address was made public, and ever since then he's been forced to take his family and flee his home. He was also fired from his job as CFO with Vante. A lot of this is karma, but I simply can't get into the whole death threat thing. I'm not a leftist. It's what they do. Otherwise, Smith tried to see the female employee he bullied at the drive-thru window and she refused to see him. I say good for her! So he took to Youtube and posted this lackluster 'apology' and 'clarifications':
On the bright side, I saw over at Gateway Pundit that a site to support Rachel. The site's webmaster(s) stopped by Chick-Fil-A in Tucson and gave Rachel some flowers.
http://www.fireandreamitchell.com/2012/08/03/chick-fil-a-bully-adam-smith-tries-to-apologize-to-female-she-refuses-to-see-him/Latest Ramblings
So given all the fallout, I wanted to know if he cared to see Rachel again. "If you encountered her again, what would you say to her?" I asked.
"I will say to her… what I will say to her… and I will figure out what the right words are," he responded. And then he shed a little more light, saying that he had already gone back to Chick-fil-A the next morning to talk to Rachel.*
She refused to talk to him.
"I wanted to see if she was OK, that's all," he explained. "She was actually working there, and her manager went to get her… and she wouldn't come back."
Considering the controversy that's erupted, you can't really blame her.
By the way, we have repeatedly reached out to his former employer Vante for comment and have not yet received a reply.


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subset: commoncrawl


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MUMBAI: Deepak (name changed), a Kolkata-based employee of Vodafone India, has more on his plate than what his job profile demands. He has to undergo a rigorous driver's training, which includes how to drive safely, and running checks on seat belts and helmets.
Such training is now mandatory and those who don't undergo this, can get a bad appraisal, and even worse, lose their jobs. Over a year ago, three employees were fired by Vodafone India for driving recklessly out of office hours.


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|New Pax Flights To/from Anchorage. posted Fri May 20 2005 20:57:34 by Sokol
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Don't Miss: October
by nycgo.com staff, 09/17/2013
Photo: Julienne Schaer
Ice-Skating Rinks Open
Ice-skating is synonymous with winter in New York City. Luckily, you don't have to wait until the holidays roll around to enjoy this time-honored tradition. Many of the City's rinks open early in the fall—including Bank of America Winter Village at Bryant Park, Trump Rink in Central Park (formerly the Wollman Rink) and The Ice Rink at Rockefeller Center—and others are open year-round, like the Sky Rink at Chelsea Piers, City Ice Pavilion and Aviator Sports & Events Center. Taking advantage of the early openings may be wise, as crowds tend to fill up the more popular rinks later in the year.


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Should Vietnam veterans have their discharges upgraded for PTSD?
Sort of an interesting article in the New York Times today about veterans of Vietnam who were discharged with less than Honorable Discharges who want to upgrade their discharges on the basis of PTSD. Bear in mind that PTSD as a mental diagnosis didn't exist until 1980, so this determination would have to be retroactive. The Yale Law School Veterans Legal Service Clinic has filed suit, and as the NYT lays out, the court has to address two issues:
The suit raises two thorny issues that could affect thousands of Vietnam veterans: Can they be given a diagnosis of PTSD retroactively, to their time in service, though the disorder was not identified until 1980? And if they can, should recently instituted policies intended to protect troops with PTSD be applied retroactively to their cases?
The article talks largely about one such individual, John Shepherd Jr., who had a mixed record in the military:
Within a month, his patrol was ambushed, and Mr. Shepherd responded by tossing a hand grenade into a bunker that killed several enemy soldiers. The Army awarded him a Bronze Star with a valor device, one of its highest decorations.
Yet the medal did little to assuage Mr. Shepherd's sense of anxiousness and futility about the war. A few weeks after his act of heroism, he said, his platoon leader was killed by a sniper as he tried to help Mr. Shepherd out of a canal...
After a court-martial, the Army discharged Mr. Shepherd under other-than-honorable conditions, then known as an undesirable discharge. At the time, he was happy just to be a civilian again. But he came to rue that discharge, particularly after his claim for veterans benefits was denied because of it. It was a breaking point: his behavior became erratic, and at some point he simply refused to go on patrol.
So, I went to the Yale Law website about Mr. Shepherd's case, and they certainly lay out a comelling argument. One thing that might factor against Mr. Shepherd is that apparently he had problems prior to even arriving in Vietnam, including going AWOL from Basic Training for almost a month.
The last sentence of the article doesn't seem to me to help his case much:
"I want that honorable," he said. "I did do my part, until I really felt it wasn't worth getting killed for."
While I understand what he is saying, I'm not sure you can leave it to individual soldiers to determine when something is "worth getting killed for." But even if it isn't worth getting killed for, I'm not sure that neccessitates upgrading the discharge.
Nonetheless, it should be an interesting case. Had this happened today, his discharge probably would have been upgraded. But then again, if he had gone AWOL from basic today, he probably would have been separated before even deploying, so I am not sure that is the determinitive factor.
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Richard Mason (not verified)
February 1, 2013 - 7:56pm
In the early 1990s I was involved in helping to set up a Korean War Veterans group in Miami, Florida.
After some newspaper publicity, we had a meeting with about 40 KW Vets in attendence. The next month's meeting we had about 30 vets. Then the following month's less than that. I started running into a few of the men at the VA Hospital that had attended the early meetings and asked them why they had not continued to attend. They all told me that after leaving the military in the 1950s, they went to school, got married and raised a family and surpressed any thoughts of their comcat experiences. After attending the KWA meetings they started getting flashbacks of the Korean War and they were being over whelmed with anxiety. sleeplessness and all the nervousness. They went to the VA and were in the mental health clinic.
What does that tell you about delay PSTD.
David Turner (not verified)
June 6, 2013 - 12:55pm
Veterans Discharge Upgrades
I am presently working with Senator Bernie Sanders (Chairman of the Veterans Affairs Committee) on submitting a proposal to the Dept. of Defense to give an overall pardon to any Veteran of Vietnam who received an "Other than Honorable" discharge, based only on the criteria that they have lived a "reasonable" life since their discharge and not based on mitigating facts that slow down the process and in many cases become reasons for denial. Anyone wishing to help may contact his Veterans affairs officer Sam Haskins
My Crew Chief, the man I owe my life to, deserves to at last feel his sacrifice was worth at least something to this country!
Robert (not verified)
February 3, 2014 - 12:48pm
upgrade discharges with ptsd
I have fought forty years for upgrade I have begged va for help. Five suicide attempts ptsd, depression,copd erectible dysfuction ten other medical ailments. It has been a long and hard road. I would not wish this on my worst enemy. I ask to see a military phycharist while I was in service but they refused to see me. This is one hell of a death sentence.
Steved (not verified)
May 24, 2014 - 4:28pm
don't know how to get help
I was in vietnam in 1968, during that time my grandmother was on her death bed and i ask and got a compassonate leave. but i got back to the departure station two days late and the mps were called in on me..... they told me that when i got back to my company i would be courtmartialed and put in prison or firing squad...... a 19 year old boy and it scared the hell out of me so when i went to the mess hall i kept going... i did not hide i went home and went to work.... in 74 i applied for the amnisty offered by ford.... the officer they put me with as a defense told me to sign the paper or i would be found guilty and sent to levenworth.... i signed and have a undiserable discharge. I don't know how or if i can get it upgraded but i would like to get it done before i die..... i'm 67 now... at the time i was not affraid of the vietcong or the nva... but i sure as hell was scared to death of what my own military would do to me for being two days late reporting in and with the mps..or cops told me would happen at that time was the way it would happen.... police back then of any type were not to be fooled with... they told the truth and were beyond being questioned on their orders... so i left now i want to fix it if i can.... i was told to do comunity service and for the last 45 years i have played for any type of fund rasing to help people every chance i got... oh... i play music and donate time to help when i can..... if any one knows how i can get help please let me know...... stevepennington AT charter dot net
Anonymous (not verified)
June 5, 2014 - 9:54am
VA Treatment
I was Medivac to Philaphila VA 1971 for a skin problem.And they wanted me to help clean the hospital.I refused and the doctor recommended to for me full duty.At the Naval Yard.I FIGURED I BETTER MAKE A COPY OF MY COMPLETE HEALTH RECORD.To keep them from saying they couldn't find my records in the future.So the system have been misleading veteran for years.Especially the lower level military enlisted.I'm sure the higher staff officers knew.They were the doctors.Go back to Nixon and work your way up.I have the proof to show.SEMPER FI


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Ancestors: Famous and Notorious
Meet two more of my Rhode Island ancestors, one famous and the other infamous, even notorious in his day. Times change and notorious conduct 400 years ago is a cause for admiration today. Many who were persecuted in colonial times are responsible for the freedoms we now take for granted.
Roger Williams was born London in 1603. His father was an importer and trader, a merchant of some importance until his death in 1621. Roger grew up during the period when numerous Puritans and others thought to be heretics were burned at the stake at Smithfield near London, acts that influenced his later religious beliefs. He was educated at Cambridge University's Pembroke College and graduated in 1627 having received scholarships for his excellence in Latin, Greek and Hebrew.
As a theologian and independent preacher, Roger Williams first preached at Salem, Massachusetts in 1631. His controversial beliefs in separation of church and state and freedom of religion attracted the attention of Massachusetts Bay's Puritan rulers who were about to deport him back to England in 1636. He fled southwest to the area around Narragansett Bay where he founded Providence, the first area of Rhode Island settled.
Williams founded the first Baptist church in America, the First Baptist Church of Providence. He favored treating the Native Americans fairly and was a peace maker between the colonists and Indians. He established a trading post at North Kingston, and as governor of the colony from 1655 to 1658 encouraged trade with the Indians. Williams may have been the first American abolitionist as he forbade slavery in the state of Rhode Island. His belief in separation of church and state would become a founding principle of our American form of government.
His contemporary, Samuel Gorton was born in 1591 in Manchester, England. Also from a wealthy, influential family, Gorton received a liberal education. His fluency in Greek and Latin enabled him to read the Bible in its original text leading to his nonconformist beliefs. A convincing speaker, he would speak wherever people would listen to him, although it was said that he "did indeed clothe his thought at times, in clouds, but then it was because they were too large for any other garment."
Gorton brought his family to Boston in 1636 and was constantly in trouble with authorities. After he moved to Portsmouth, Rhode Island two Indian chiefs, Ponham and Soconoco, were hired by Massachusetts to raid Gorton's home and burn it down. In 1642 after several run-ins with Massachusetts authorities, Gorton and 100 of his followers were put out and forced to walk in a blinding snowstorm about 90 miles back to Providence. Massachusetts continued to harass him until he left his family and went back to England for three years.
There the Earl of Warwick befriended Gorton and helped him obtain a royal charter from the king as well as an order of safe passage. He returned to Boston where authorities had to honor his documents and allow him to proceed to Providence. Gorton founded Warwick, Rhode Island, and was elected the first President over the towns of Warwick and Providence, called the Providence Plantations. He was elected a Deputy Governor in 1664, 1665, 1666, and 1670. Treated as a heretic by the Puritans, he wrote, Hypocrisy Revealed, a critique of his critics. A graduate of Pembroke College and Cambridge University, Samuel Gorton was in fact a minister of the Gospel, an independent thinker and a true champion of liberty.


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Jul 30, 2009
According to industry sources the largest decrease will take place early next week, when six plants are expected to down, reducing production by about 23%.
The reduction comes at a time when the USA is producing more pork than it can use. A slowdown in pork exports due to the global recession and fears over the novel influenza A virus (H1N1) has put an excess of the meat in domestic channels, said Glenn Grimes, agricultural economist at the University of Missouri.
Pork plants
At least two pork plants will be down on Friday and up to six will be down on Monday, according to livestock dealers who monitor pork production. Two of next week's closings will be due to floating holidays.
On Friday, Smithfield Foods plants in Sioux Falls, SD, and Sioux City, IA, are scheduled to be down, dealers said.
Next week's scheduled closings currently include the Smithfield plants in Clinton and Tar Heel, NC, and in Smithfield, VA; the Hormel Foods plants in Austin, MN, and Fremont, NE, and the JBS-Swift plant in Worthington, MN.
Related websites:
Smithfield Foods
University of Missouri
Hormel Foods
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Vincent ter Beek
May 19
Dick van Doorn
May 18
David Burch
May 23
Vincent ter Beek
May 20
Vincent ter Beek
May 11
Vincent ter Beek
May 9


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Dead Space 3 is upon us and we have the full list of Kinect voice commands you will be able to use throughout your journey.
The game is enhanced with Xbox 360's Kinect hardware that constantly listens to your voice. Giving the console some vocal commands can make playing Dead Space 3 all the more easier and more seamless, as you can avoid pressing buttons in combination using this system.
So, whether you wish to open your inventory, reload a weapon or help out a friend in Co-op, these Kinect voice commands will free you up to experience the horror that awaits in Dead Space 3!
Basic Interface Actions
- "Find Objective" – locates an objective
- "Find Suit Station" – locates a Suit Station
- "Find Bench" – locates a bench/workstation
- "Access Inventory" – Opens inventory menu
- "Access Database" – Opens Database menu
- "Access Mission" – Opens mission menu
- "Scavenger Bot Status" – Opens the Bot Status menu
- "Exit" – Exits opened menu
Gameplay Actions
- "Reload Weapon" – Reloads current weapon>
- "Switch Weapon" – Switches to the next available weapon
- "Quick Heal" – Uses the health pack
- "Refill Stasis" – Uses the stasis pack
- "Switch to Scavenger Bot" – equips the scavenger bot
- "Pick up Item" – Picks up an item
- "Use Stasis" – Uses stasis while aiming
- "Grab Object" – Uses TK Grab while aiming
- "Throw object" – Uses TK Launch while aiming
- "Get off me!" – Paired attack escape
Co-op Actions
- "Give Partner Health Pack" – Gives your partner a health pack
- "Give Partner Stasis" – Gives your partner a stasis pack
- "Give Partner Ammo" – Gives your partner an ammo clip
- "Help me!" – Calls your partner for help
- "I Need a Health Pack" – Ask partner for a health pack
- "I Need a Stasis Pack" – Ask partner for a stasis pack
- "I Need Ammo" – Ask partner for an ammo clip
- "Find Partner" – Locate a co-op partner
- "Revive Partner" – Revive your partner
Dead Space 3 is out now on Xbox 360 and PlayStation 3. Look out for the Aussie-Gamer review next Wednesday!


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Nov. 2, 2009
When senior Lady Techster volleyball player Samantha Crowder was asked what it is she brings to the team, with no hesitation she replied, "Comedy."
"I make everybody laugh," Crowder said. "There is not a day that goes by that I don't make somebody on our team laugh. What can I say, I'm a goofball. Sometimes it gets me in trouble, but I think it's all worth it."
Although Crowder has a lively, comedic personality it doesn't take long in talking to her to figure out she brings much more to the Lady Techsters than just comedy.
The senior has shown she is not just comedy while on the court, recording over 500 kills in her career and she currently ranks in the top ten in Tech history for career blocks. Crowder has played a pivotal role this season in helping the Techsters to their best start since 2005 while leading the team with a .288 hitting percentage. Tech head coach Matt Sonnichsen believes she is a rare, complete player at the middle blocker position.
"Sam can hit any of the middle attack options, blocks extremely well, has quick footwork and an excellent vertical jump," Sonnichsen said. "She has been a great senior, providing a happy demeanor to keep her team mates relaxed during the challenges of our season. It is a big plus for our team to have the talent of Sam available and she has excelled at coming into games at key junctions to provide a big swing or block."
In addition to her ability on the court, Crowder provides a different element off the court for her team in leadership.
"I try to be like a big sister on the team," Crowder said. "I'm always giving advice to teammates and they know I'm always there for all of them. If someone is down I'll be there to encourage them and make them feel better and I'm always there to deal with any issues within the team. I'm open with everybody so anybody who knows me knows everything about me which I think makes it easier for them to open up to me and tell me if something is wrong."
The Floresville, Texas native says she shares this big sister role with her senior teammates Lindsay Armstrong and KC Clayton as well.
"I think this senior class has incredible leadership abilities," Crowder said. "I think it's because we've been through so much together. We have been through the worst of the worst of the worst together here at Tech and now that we are having some success we know we can't take what we have for granted. We don't let anybody on the team take anything for granted either."
Crowder also illustrates her passion for her school and the game while reminiscing about Tech's home opening tournament earlier this year. In the final match of the tournament Tech had to beat South Alabama, who was undefeated at the time, in straight sets to be crowned tournament champions and that is just what they did topping the Jaguars 25-20, 27-25 and 25-22. Crowder led the team with an impressive .500 hitting percentage in the win over USA.
"Knowing the only way we could win the championship was to win in straight sets and then to pull it off was a great feeling," Crowder said. "Coach Teresa Weatherspoon (Techster basketball coach) came in and gave us an unbelievable pre-game speech stressing the importance of winning in front of your home crowd, defending our home court and the pride we have in our program. Everything she says gives me goose bumps, she is so inspiring and I think the whole team felt that way. It was just an amazing feeling being able to hold my finger up to the alma mater in pride after a win like that."
Comedy is defined in Merriam-Webster's dictionary as simply humorous entertainment. It's pretty safe to say that Tech's Samantha Crowder brings much more to the table than just humorous entertainment.


text: I managed to fit some 700x32 ritchey speedmaxs on them. Aside from mud clogging up my calipers, anyone see a problem with this? will i damage my road bike? Also, I have a pair of mavic cosmos with 24F/28R and I weigh about 165. Will that work out or should i use 32f/32r?


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Imperial County officials hoping for quake relief
El Centro, Calexico suffered the worst damage in temblor
The American Red Cross and the Salvation Army have been providing assistance to quake victims. Here is where monetary donations to support that work can be made:
American Red Cross San Diego/Imperial Counties Chapters Disaster Fund at sdarc.org or by mailing a check or money order to American Red Cross, 3950 Calle Fortunada, San Diego, CA 92123.
Baja/Imperial Earthquake fund at the Salvation Army, P.O. Box 503580, San Diego, CA 92150-3580. People also may call (866) 455-4357 or donate online at www.sandiego.salvationarmy.org.
As they prepare to receive Gov. Schwarzenegger and U.S. Sen. Barbara Boxer today, authorities in Imperial County are looking for an infusion of federal and state emergency aid to address extensive damage caused by Sunday's 7.2-magnitude earthquake, centered about 30 miles across the border in the Mexicali Valley.
Though it claimed no lives in the United States, the temblor took its toll on a broad range of businesses, residences and public facilities, with El Centro and Calexico suffering the most widespread damage. Shops, homes, schools, water tanks, sewage systems and government facilities have been affected.
"We're still in our damage-assessment mode," El Centro Mayor Cheryl Viegas-Walker said yesterday, reporting $8 million in residential damage, $20 million in commercial damage and about $10 million in industrial damage. A water-treatment plant damaged in the quake will cost $7 million to repair.
The quake threatens to take a severe economic toll on the county of about 170,000 residents, where unemployment hovers around 27 percent. In Calexico, with 38,000 residents, City Manager Victor Carrillo estimated job losses from shuttered businesses at 3,000 to 4,000 and infrastructure damage to the city at about $23 million.
"With all these unemployed people, small businesses shut down, schools closed, it's not a pretty picture," Rep. Bob Filner, D-San Diego, said yesterday after meeting with mayors, county supervisors and other Imperial County authorities. "It's going to take a while to recover."
Filner expects to join Schwarzenegger and Boxer today as they survey the damage.
Schwarzenegger already has declared a state of emergency in Imperial County as a result of the earthquake. Filner is asking that the governor seek a presidential disaster declaration for Imperial County, allowing the release of federal emergency aid.
The brunt of the quake's force struck Mexico, with 25,000 people left homeless and two fatalities in the Mexicali area. In Imperial County, a man in El Centro was seriously injured when a sign fell on his head, and he was flown to San Diego for treatment, the mayor said.
In Imperial County, some of the hardest-hit residents appear to be those living in mobile homes, many of which were shaken off their foundations. County Supervisor Gary Wyatt estimated that several hundred mobile-home-park residents have been forced from their homes. In El Centro, Viegas-Walker said 52 people have been displaced as a result of damage to mobile-home parks.
County officials are working to help those people safely return home. "We want to make sure when they are put upright that they are safe and all of the utilities are properly connected," Supervisor Wally Leimgruber said of the mobile homes. "With summer months approaching, we want to make sure all the air-conditioning units are operating."
By yesterday evening, only 16 people had sought refuge at a county shelter set up at a junior high school, said Maria Peinado, spokeswoman for the Emergency Operations Center of Imperial County. She said the office was preparing a statement last night that would estimate the damage.
Damage to water tanks caused problems in Calexico and El Centro, and both cities reported damage to sewer systems, leading to a temporary decrease in capacity.


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Celebrate Kindness Week will be held February 11-17, 2007. Celebrate Kindness Sunday is February 11th. An annual campaign, the goal of the Celebrate Kindness campaign is to promote goodwill and peace in our communities through the sharing of kindness with friends, family, neighbors and even perfect strangers. This year's theme is "Cool 2b kind!"
Celebrate Kindness is coordinated by MeritCare Community Connections in cooperation with public and private schools in Fargo, Moorhead, West Fargo, Mayville, and the Bemidji region. Throughout the week, schools, faith communities, and businesses in the region are encouraged to find opportunities to intentionally spread goodwill through kind words and service to others in the hopes that the doing and receiving acts of kindness will set a tone of peace for the year to come.


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Wednesday, September 25, 2013
11 Comments | Copyright: India-Forums.com / TellyBuzz
Ulka Gupta who is barely 16 years of age and is pursuing her 12th boards from Mithibai College in Arts wants to become an ace Bollywood actress. She is currently seen in Khelti hai Zindagi Aankh Micholi as a sweet and naughty Ami. Earlier she also played the role of Ek Veer Stree Ki Kahaani Jhansi in Jhansi Ki rani in Zee TV.
TellyBuzz had a candid conversation with Ulka who shares about her Bollywood dreams, her source of inspiration towards acting and her journey in the industry.
Here it is what Ulka said...
How did your journey in this industry begin?
I started acting in a very early age when I was barely 8 years old. I did couple of advertisements and shows then. Later I gave an audition for Jhansi Ki Rani and got selected and then I Khelti hai Zindagi... happened.
Who was your inspiration behind becoming an actress?
My father was my inspiration and source of motivation as he himself is an actor in films. His name is Gagan Gupta. Being a daughter of an actor itself feels good and somewhere the genes do come in.
In which standard are you studying?
I am pursuing my 12th boards in Arts stream and doing correspondence from Mithibai College.
How important is it for you to complete your education?
For me education is very important and I respect it and I will pursue BA post my 12th and if possible I would like to get into Masters of Politics.
How do you cope up with studies and shoot?
Well, I usually try to balance time out by taking leaves whenever required. It's not that tough as I have to juggle between my studies and shoot.
How is it working with Gautami Gadgil?
To be honest, I thought that she is Mrs. Ram Kapoor so I had a sense of feeling that she would somehow be very different or may have an attitude but I was amazed with her perfection and her kindness. We share an amazing rapport with each other. In fact we actually rehearse our lines together and we also help each other in many ways as we both portray the roles of mother-daughter in the show. So we help each other in understanding the bond of togetherness. She is the most amazing human being and a very good co-actress.
There is a sudden transition in your role from Ek Veer Stree Ki Kahaani Jhansi Ki Rani to Kheta hai Zindagi...was it difficult for you to portray this role?
I feel Jhansi Ki Rani was one of its kinds where I had to portray a role of a typical empress which was very different and somehow I have practiced and learnt a lot from that show. I used to read books to polish myself to get into the skin of the character while the role of Ami is very much a younger version of myself. The way today's teenage girls are presented on screen, this 16 year old girl Ami acts and behaves like a 12 year old girl as she is too protected by her family. But every girl would certainly relate to the character of Ami.
Whom do you get along with the most on the sets?
I get along with everybody on the sets. Manav Gohil as an actor is very nice. He is also very jovial but my tuning with Gautamiji is immensely high and I also have sweet moments with my on screen sister Khusboo. She is very mischievous. In fact we both are mischievous.
What is your ambition?
I want to be a successful actress in Bollywood. I may not have good height but I do have a sense of good acting skills. I know the trend these days are of tall girls but acting ability is also must. There were yesteryear actors like Jaya Bacchan, Waheeda Rehman who are not tall too but still are very famous. I too want to make it big in Bollywood.
Which television shows do you like watching?
I usually don't get time to watch television. Whatever time I get, I go home and either study or rest but I do watch my own show.
How similar is your character in real life?
I am more like Ami in real life, but I wasn't talkative at all. After essaying the role of Ami, I have started talking. I mostly used to be very focused. In fact I gel with most of the people and at times I don't open up so you can say I am an introvert.
Moreover one thing that my relatives keep on telling me is that I am way too grounded and I just don't actually have any celebrity like feeling. I should mostly have some kind of attitude and act like a celebrity. I give my due time to everybody which people think I shouldn't.
Had you not been an actor what would you be?
I am very much interested in Politics so I want to become a politician. Had I not been an actor I would have tried my hands at politics.
If you get an offer wherein you have to play a main lead in a typical daily soap like Avika Gor is doing in Sasural... will you accept it?
No, I will never accept it. I want to make it big in Bollywood and I want to play the roles referred to that of my age. This happens to be my last show on television. I would want to pursue my Bollywood dreams for now.
What kind of offers were you getting?
I am actually getting the roles of female leads but I was not very keen to take the offers as I wanted to prepare myself for Bollywood. I want to become an actress. If I take up the role of a female protagonist then it would be typecast and I don't want it to be.
What do you usually do during your free time?
I mostly read books, sleep and I love playing with kids in my building. I am a boxer and love swimming as well. I am a sports person and this will groom me better towards making me an actress. I also know Horse riding as I had to learn it during Ek Veer Stree Ki Kahaani Jhansi Ki Rani.
Ruchita Mishra
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ATLANTA, Ga. -- Friends of an Atlanta kickboxer are mourning her death during a tournament.
Adrienne Simmons, 34, died Monday after being struck in the head during her match on Sunday in Orlando.
"She took a left hook to the head and went down," said an Atlanta friend, Nick Dekay.
Simmons was knocked unconscious during the final round of a three-round fight.
Simmons' boyfriend, Chike Lindsay-Ajudua of Atlanta, told 11Alive News that there was no ambulance and there were no paramedics standing by at the tournament Sunday, and that it took an hour for Simmons to get hospital treatment.
"It took over half an hour after the incident for her to even be loaded onto a gurney for transport," Lindsay-Ajudua said. "Transportation took another 20 minutes, which gave over an hour of time for the swelling of her brain to get to a point where there was little that doctors could do.... The severity of this accident is a direct result of the proper precautions not being taken by the IKF [International Kickboxing Federation]."
As of late Tuesday night, the president of the International Kickboxing Federation, Steve Fossom, had not returned messages that 11Alive News left for him.
All that Adrienne Simmons' friends in Atlanta know is how much they admired her, how much they'll miss her.
"She was fully aware of the risks inherent in this sport, as we all are," said Nick Dekay. Simmons trained and worked for a while at Dekay's midtown Atlanta gym, Unit 2 Fitness.
He said he understands what attracted Simmons to kickboxing.
"You overcome adversity in this sport," Dekay said, "and if you've ever felt powerless or weak before, this kind of training can really change that."
It was her way of building self-confidence, he said.
Atlanta Kickboxer Dymond Jones said Simmons was a fierce competitor.
"I've seen her come off of losses and still want to get in here and train," Jones said. "And that's a testament to her spirit, you know, no matter what, she still wants to train, she still wants to work harder, and be better."
The Sheriff of Orange County, Florida concluded that Adrienne Simmons' death in the ring Sunday was an accident.
She was doing what she loved, her friends said, in their grief -- and she was looking to her future.
"She was looking to be a very, very high-level female competitor," Dekay said "But additionally, she was doing her Master's Degree to get her teaching -- so she could teach" in the public schools.
The co-promoter of Wild Bill's Fight Night, David Oblas, said Simmons fought in one of his matches about two years ago.
"She was extremely easy to work with and always was laughing, smiling and having a good time." Oblas said. "She was one of those people that you naturally just gravitated to hang out and chat with. She always seemed to enjoy where she was at and even joked minutes up to her fight in the dressing room."
Oblas, host of 790 The Zone's Counter Punch program, said the "combat sports community of Atlanta" will miss her.
Simmons' family is in Alabama. Her friends in Atlanta plan to hold a memorial service for her in Atlanta.


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Max Payne Movie
|Release Date: October 17, 2008
|Running Time: 100 mins.
|MPAA Rating: PG 13
|Box Office: $40,687,294.00
|Genre: Action, Adaptation, Adventure
Mark Wahlberg
Mila Kunis
Beau Bridges
Chris Bridges
All Cast & Crew >>
Summary:Max Payne is a maverick cop with little regard for rules and nothing left to lose. Hell-bent on revenge, he's determined to track down those responsible for the brutal murder of his family, but his obsessive investigation takes him on a nightmarish journey where dark fantasy collides with stark reality. As the mystery deepens, Max is forced to battle enemies beyond the natural world and face an unthinkable betrayal that will drive him to the edge of his own sanity.
Event Photos:


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Edition: 7th
Author(s): Bayes, Nenna; Becklin, Karonne; Crist, Bonnie
ISBN: 9780073401980 / Paperback / 1/13/2011
Marketplace Disclaimer:
Edition, Cover Design, etc may vary from the eCampus.com description. Please refer to the seller's comments before ordering. Marketplace sellers are required to ship by the end of the 5th business day. Items are not returnable to eCampus.com. Orders cannot be cancelled by eCampus.com.


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All branches closed for Memorial Day, May 30th.
The pursuit of love ; & Love in a cold climate : two novels
The Radletts of Alconleigh occupy the heights of genteel eccentricity, from terrifying Lord Alconleigh (who, like Mitford's father, used to hunt his children with bloodhounds when foxes were not available), to his gentle wife, Sadie, their wayward daughter Linda, and the other six lively Radlett children. Mitford's wickedly funny prose follows these characters through misguided marriages and dramatic love affairs, as the shadow of World War II begins to close in on their rapidly vanishing world.
Call Number:FIC Mit


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Art Modell: A complex man
Art Modell, a man well known to long-time National Football League fans, especially those from Ohio, passed away last Thursday at the age of 87. He was foundational in the growth of the NFL both as a league and as the entertainment juggernaut it is today. But even as they read this, Cleveland Browns fans are either wincing or gnashing their teeth. They knew Modell as a traitor, a Benedict Arnold, even a thief. His death marks the passing of an NFL icon, a pioneer, and more than a bit of a pariah.
In 1961 Modell purchased the Cleveland Browns, and in his first 10 years of being owner, he changed the landscape of football forever. He helped establish the first collective bargaining agreement, creating a fabric for the NFL's finances that still exists. Then came one of his greatest accomplishments, and certainly the one we can most easily recognize today. In 1970, along with the legendary league commissioner Pete Rozelle, Modell helped to push through the contract between the NFL and ABC for what we still know today as Monday Night Football. It's safe to say that the NFL we love to watch on TV wouldn't exist without Art Modell.
But 1996 is a year that will forever stick in the craw of Northern Ohio residents. Cleveland was a championship-starved and snakebit sports city (and still is). But unbeknownst to the long-suffering fans, Modell was secretly reaching a deal to move their beloved Browns to Baltimore because of his financial situation. It ripped the soul out of a diehard fan base. He stole their team right out from under their noses. To this day Art Modell is roundly cursed in Cuyahoga County. Even the 1998 introduction of a new Cleveland Browns team via expansion hasn't done much to dull the hatred.
More than just a big businessman, though, his players knew him as a great man. Hall of Fame tight end Shannon Sharpe said, "He showed real concern. But, it wasn't just me. He knew the practice squad players' names. He treated them the same." Current all-pro linebacker Ray Lewis described Modell this way: "Every minute of his life, he cared more about everyone around him than himself. … I genuinely loved Art as a man, and he showed me what to strive for in life. … He was a humble servant, and one of the best men I have ever known."
Now he is gone. What are we to make of a man who simultaneously inspired loathing and love? I suppose we are to remember that he was but a man. His life was marked by profound common grace, which enabled such pivotal successes. And his life was marked by the failure inherent to all men. Art Modell was a complex mix of good and bad, of grace and greed. And in this he was not so different than you or I.


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July 1993
Christa Wolf is an East German novelist and essayist has lived through both National Socialism and "Real Socialism." Her insights into the mechanism of co-optation and surveillance under totalitarian rule have translated into complex literary works and thoughtful essays on politics, culture and the life of the individual in a stultifying society. Books by Wolf include The Quest for Christa T., A Model Childhood, Cassandra, and What Remains?
Last Updated: 9/14/09


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South Korea's Next Cultural Wave Could Be Smoothies
A South Korean cultural wave is sweeping the world, with interest surging in areas ranging from the country's pop music to cosmetics to television dramas. Now, smoothies could be next.
At least that is what Standard Chartered's private-equity unit is banking on with a $45.5 million joint investment with Korea's National Pension Service for a 48% stake in Smoothies Korea.
The Korean fruit-blended beverage maker is trying to position itself as a healthful Starbucks-like competitor in the world of smoothies. Its 100 company-owned and franchised stores tap into the country's café craze while offering a healthy alternative to coffee and shunning sugar as a sweetener, said Charles Huh, managing director of Standard Chartered Private Equity's Korean arm.
The plan is to replicate the café approach in the U.S., where grab-and-go is currently the norm, he said.
As part of its new strategy, Smoothies Korea will buy out the American company behind the Korean franchises, New Orleans-based Smoothie King, which began in 1987 and has 529 stories in the U.S. Its owners have agreed to sell.
The Korean company also intends to open stores in China and Singapore, both new markets.
Smoothies Korea hopes to follow the success of other Korean food and beverage brands that have gone overseas, such as Paris Baguette, a bakery and coffee chain with branches in the U.S., China and Singapore, and Red Mango, a frozen yogurt and smoothie chain with a presence in the U.S., Southeast Asia and the Middle East.
Smoothies Korea, set up in 2003, is growing rapidly domestically as Koreans become more health-conscious and gradually shift away from consuming caffeinated drinks. While strawberry is its most popular flavor, there are also local blends such as "red ginseng vitality," based on a root native to the country used as an energy booster. Deal Journal has more.


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Meet and Communicate with Fun Singles Near You on JDate
Register now to search thousands of members and post your FREE profile!
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I love life.....Oklahoma is a wonderful state if you haven't visited! I stay super busy----but if I like ya----I will e-mail you back....
mozart4u is a 48yr-old single woman in Shawnee, OklahomaView Profile
Shalom. My name is Joshua and I live in Moore Oklahoma, just outside Oklahoma City. I just started attending the Reform Syng. in OKC ...
YeshuaA562 is a 34yr-old single man in Shawnee, OklahomaView Profile


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Here are just a very few of the kajillion maps I made from about 11 yrs old through my younger teen years and even my first digital maps!! I can't believe i still have these. I notice one has a reference to Darokin from D&D's Mystara module.. never played but i liked that world map.
Most were made the old fashioned way with pencil and paper and there are a couple forms to show how i kept track of populations etc.. i changed them as i grew the cities so often the paper would wear through from erasing. That one form (last attach.) was made from rub on decals and what i though was a "finepoint" back then... boy was I proud of that hideous form LOL!!
These weren't for show but for my own enjoyment.. and i have notebooks and loose sheets of them stashed all over... as well as rolled up giant size and ever expanding graph paper maps i would lay on and draw for hours. Many have gone missing but I have enough to go through and muse at from time to time. Keeps me in touch with the roots of my mapping passion


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I have been playing The Elder Scrolls for along time, ever since Daggerfall came out. Truely RPG on the market based on single play, but lack of multiplay reduced the interest and hangin in there. Some enjoy it as loner without MP.
Looking forward to this one...
I have played Gothic4, Two Worlds 2, etcs, none please me more than this one. Just wanted something that is epic and realistic


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Upstart Improved Documentation and New Features for Ubuntu Natty Release
"Jobs and Events are the primary Upstart concepts," writes James Hunt Ubuntu Upstart maintainer at Canonical. "The version of Upstart provided with Ubuntu Natty provides a new "initctl" command "show-config" which when coupled with a new tool "initctl2dot" allows these interactions to be understood visually."
In this post today, Hunt explains the new "initctl show-config" command as well as the "initctl2dot" command and show in a series of visualization graphs how the typical Ubuntu system starts.
Hunt uses his blog to explain to users what Upstart really means and highlights a few new commands. In his series of posts on Upstart Hunt shows that new with the Ubuntu Natty cycle is a focus on documenting the mysteries of Upstart.
For users who want to know more about Upstart, how it works, and see the full documentation can start with Hunt's blog post.
Issue 187/2016
Buy this issue as a PDF
Linux is the Largest Software Development Project in History
But if you are not using the latest Linux kernel, your system is insecure.
Linksys Says Yes to Open Source Firmware
Home routers will give room for custom firmware but still comply with FCC rules
OwnCloud Founder Resigns
Frank Karlitschek will continue to lead the open source ownCloud project
Ubuntu 16.04 LTS released
"Xenial Xerus" comes with a new packages format and several improvements for the enterprise.
MS Visual Studio Code Editor is Available for Linux
Linux users can now download and install the Windows code editor
Open Container Initiative announces Image Format Project
New initiative will address security and interoperability concerns around container technology.
Red Hat Lures Developers with Free RHEL and Container Development Kits
Developers can use RHEL as a development platform without a subscription fee.
Microsoft Brings the Linux Command Line to Windows
Windows users will soon have native access to the Bash shell.
Internet Research Group Proposes Better Email Standard
Improvements to SMTP will provide better guarantee of confidentiality
New Nvidia Driver Offers Wayland Support
Graphics vendor embraces new reality in Linux graphics


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|US Airways: CLT-LCQ And PHL-LPL? posted Thu Aug 24 2006 23:09:26 by SHUPirate1
|US Airways Eyes PHX-FRA, PHL-ASIA ULH Route posted Thu Mar 29 2007 18:49:03 by Jimyvr
|US Airways Alpa Billboard Near CLT posted Mon Dec 18 2006 17:28:44 by Adam T.
|US Airways Express To Begin CLT-HPN, End PIT-HPN posted Mon Sep 18 2006 17:54:48 by A330323X
|US Airways PHL-SLC Or CLT-SLC? Why Not? posted Fri Jul 14 2006 22:17:59 by Slcdeltarumd11
|Why No CLT-MDW Service By US Airways? posted Wed May 17 2006 18:59:41 by United777ORD
|US Airways At CLT: Flight Cancellations 5/11/05 posted Wed May 11 2005 05:40:55 by SHUPirate1
|US Airways Twice Daily To FRA? posted Tue Mar 1 2005 13:55:42 by FlyinTLow
|US Airways Emergency Landing In CLT posted Wed Feb 23 2005 17:39:30 by RyeFly
|US Airways Express / PSA To Begin CLT-GGT posted Tue Aug 17 2004 18:29:40 by A330323X


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IMO, removing the auto decompression lever would be a mistake.Without it in place and operating as it should, the starter motor, starter gear train and the sprag clutch will be subjected to much higher loads as they raise the piston against the full compression caused by the exhaust valve being fully closed.This sounds like a good recipe for part failure to me.
Can you be a bit more specific about the noise it was making? Was it a tappet-like clatter, and did it begin suddenly? Was it much worse when the engine was warmed up to full operating temperature? Was there metal coming out on the drainplug magnets?
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I have been traveling and have just learned of the planned sale of the property on N.C. 73 to the Carolinas HealthCare System for a mental health facility.
I guess I am puzzled as to why the town would be so happy to convert taxable property into non-taxable property. The half-decision to change the zoning in the first place was unusual, but at least it offered the potential for some sort of tax revenue. Now, we lose the potential tax revenue with no potential revenue for the town. The jobs won't benefit Davidson as they will not pay any taxes to the town. The property tax revenue is lost forever.
In reading some press releases, it appears as though at least some of the construction will involve doctor's office buildings. Can these be taxed? They are certainly profit-making enterprises.
–Tom MacDonald, Davidson


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Full story at 10 tonight on channel 5 on former cyclone Amanda Nisleit giving a portion of her liver to her father. Just posting to give some a heads up.
Originally Posted by BoxsterCy
Dude, you are Gameday Rulzzz and could probably post about anything.
Originally Posted by CyclonesRulzzz
Full story at 10 tonight on channel 5 on former cyclone Amanda Nisleit giving a portion of her liver to her father. Just posting to give some a heads up.
Thanks for the tip to the story. Here is the linky to the video on our awesome Cyclone!
Giving CyclonesRulzzz's post a bump because it is something Cyclones that are not even WBB fans should take a look at.
Wow! Just Wow!
Toughest player ever.
Over the years I have gotten the chance to know some of the players and their families. Amanda's folks are great people, and Amanda will always be one of my favorite cyclone Wbb players.
She loves her dad, and seeing this news report helps you to understand why. They are a great, close-knit family.
"Cómo te va", dijo el murguista a la muchacha. Que lo cortó con su mirada indiferente
Le dijo "Bien" y lo dejó como si nada
Nuevamente... La princesa... Se perdía entre la gente (Jaime Roos)
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Mr. and Mrs. Carl Oakley
Carl H. and Kathleen A. Oakley, Kingsley, recently celebrated their 50th wedding anniversary. They were married Sept. 7, 1963, in First Baptist Church of Factoryville by the Rev. Paul Gehris.
They were attended by Helene Minick Spoor and Denise Muzzy Bloxham, sisters of the bride; the late Joyce Novotka, sister of the bridegroom; the late Barry Rought, cousin of the bridegroom; the late Robert Minick, brother-in-law of the bride.
Mrs. Oakley is the former Kathleen Muzzy, daughter of the late Virgil and Edna Muzzy.
Mr. Oakley is the son of the late Russell and Eleanor Oakley.
The couple had three children, Kathleen E., New Milford; Lee Ann Bieber, Reading; and the late Steven C. They also have four grandchildren.
They marked the occasion with a surprise party at Harford Fire Hall.
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I'm new to this forum. I have a friend who is attempting to sell me his TREK Alpha 1000 . He's only had it 1-2 yrs, it's blue n white in color, 60 cm. He said he paid $600 brand new. He only rode it twice. What is a fair price to pay for this. It has all standard accessories (21 or 24 speed I think). I appreciate your reply and help. Is $200 or $250 tops a good offer to make to him? What do u recommend?
Thank You....Cameron
P.S- I was incorrect about my previous thread post..it wasn't a TREK 600


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1 Park near Shelter Cove, California
Garberville, California 9.31 miles NE
Redway, California 9.74 miles NE
Weott, California 17.66 miles NE
Myers Flat, California 19.34 miles NE
Redcrest, California 21.95 miles NE
Shelter Cove, California (map) | (707) 986-7474
Sites: 103 | Latest Rate: $46.00 | 4 Reviews
|Clean Restrooms:
|Clean Showers:
Sep 2014 - $46.00
Shelter Cove is location, location, location. It is right on the coast and drop dead gorgeous. It is only about 30 miles from highway 101 (Redway) but it is a slow drive. Road is good but narrow with many tight turns including a few hairpins and a steep descent from the mountains. Use low gear! It took us an hour and a half to do the 30 miles in a 38' class A towing a dingy. The park itself has full hookups but otherwise is minimal. Sites are close together on dirt with few trees and not level. But the view is fantastic. It sits right next to the community runway and occasionally small private aircraft take off or land but only during the day. Lots of fishermen (and women) and surfers as that is the main attraction here. No Wi-Fi and cell coverage (Verizon) is weak. Travel caution – the road between Shelter Cove and north to Ferndale (the Lost Coast) is terrible. The road itself is in horrible condition with dirt in a few spots. And since the pavement is in such terrible condition, the dirt is better. Many, many tight turns with many very tight hairpin switchbacks steep both up and down. We had to disconnect twice on hairpins. Speed is usually under 25 MPH and one still bounces all over the place. The drive is just not worth it. The view is nothing to speak of. Only a short view of the coast. Much better views can be had at Shelter Cove or Ferndale. - WillieBilly
Aug 2013 - $25.00
First of all, the 22 mile drive from Hwy 101 to this place is a real nail biter, especially pulling a 35 foot trailer. This town is a fishing village and if you don't fish ( we didn't) there is nothing to do. Campground is dismal as there are really no designated sites. No trees. We did not use the facilities except laundry and the washer and dryer were very old and rusty. Weather cold and foggy. We planned on staying for 6 nights but left after 3. Never again. - borregoannie
Jun 2013 - $25.00
The road in and out is a real tricky one, slow and easy. You're dropping to sea level here folks; those are mountains you came from! This is as rugged a get-away-from-it-all campground as you will find with accessability. A gorgeous BLACK SAND beach, nice owner, great fishing and tremendous beach combing and birding. Stayed a week and HAD to leave but WILL return. If you are into remote beaches, THIS IS IT and fairly easy to get to. - horsefolk


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Leslie Gunatilaka is Professor at the School of Natural Resources and the Environment and Director of the Natural Products Center. He is also Adjunct Professor of Department of Nutritional Sciences, and a member of the Arizona Cancer Center. He is a member of several professional societies, editorial boards, and pharmaceutical company advisory groups. He is a Fellow of the Academy of Sciences for the Developing World (TWAS), Italy, and the National Academy of Sciences, Sri Lanka. Dr. Gunatilaka has over 200 peer-reviewed publications and book chapters and over 150 communications in natural product science to his credit. He is the recipient of the Sri Lankan Presidents' gold medal for "creating a center of excellence in natural products research at the University of Peradeniya, Sri Lanka" (1987), CaPCURE award for "dedication to ending prostate cancer as a risk for all men and their families" (2000), Research Faculty of the Year Award of the UA College of Agriculture and Life Sciences (2003), the UA Asian American Faculty, Staff and Alumni Association Outstanding Faculty Award (2005), and the UA Leading Edge Researcher Award for Innovative Research (2012). He has delivered over 100 invited lectures worldwide and was the Chief Guest and Plenary Lecturer at the International Herbal Medicine Conference held in Sri Lanka (2005), and the Keynote Speaker and the Guest of Honor at Chemtech-2007, an International Conference organized by the Institute of Chemistry, Ceylon. His current research interests include discovery, identification of protein targets, and structure-activity relationship (SAR) studies of natural product-based drugs to treat cancer, neurodegenerative, and other diseases from plants, and plant- and lichen-associated microorganisms, maximization of chemistry diversity and production of microbial and plant secondary metabolites, and scientific investigation of medicinal plants and herbal supplements.
Thielavialides A E, Nor Spiro Azaphilones, And A Bis Spiro Azaphilone From Thielavia Sp. Pa0001, An Endophytic Fungus Isolated From Aeroponically Grown Physalis Alkekengi. Source: Journal Of Natural Products
June 2nd, 2014 PMID: 24882589 Leslie Gunatilaka
Four new nor-spiro-azaphilones, thielavialides A-D (1- 4), a new bis-spiro-azaphilone, thielavialide E (5), together with pestafolide A (6), were isolated from the endophytic fungal strain, Thielavia sp. PA0001, occurring in the healthy leaf tissue of aeroponically grown Physalis alkekengi. The structures and relative configurations of 1-5 were established on the basis of their MS and NMR data. Possible biosynthetic pathways to thielavialides A-E (1- 5) from pestafolide A (6), some involving a Favorskii-like rearrangement, are proposed.

Structure Activity Relationships For Withanolides As Inducers Of The Cellular Heat Shock Response. Source: Journal Of Medicinal Chemistry
March 25th, 2014 PMID: 24625088 Leslie Gunatilaka
To understand the relationship between the structure and the remarkably diverse bioactivities reported for withanolides, we obtained withaferin A (WA; 1) and 36 analogues (2-37) and compared their cytotoxicity to cytoprotective heat-shock-inducing activity (HSA). By analyzing structure-activity relationships for the series, we found that the ring A enone is essential for both bioactivities. Acetylation of 27-OH of 4-epi-WA (28) to 33 enhanced both activities, whereas introduction of β-OH to WA at C-12 (29) and C-15 (30) decreased both activities. Introduction of β-OAc to 4,27-diacetyl-WA (16) at C-15 (37) decreased HSA without affecting cytotoxicity, but at C-12 (36), it had minimal effect. Importantly, acetylation of 27-OH, yielding 15 from 1, 16 from 14, and 35 from 34, enhanced HSA without increasing cytotoxicity. Our findings demonstrate that the withanolide scaffold can be modified to enhance HSA selectively, thereby assisting development of natural product-inspired drugs to combat protein aggregation-associated diseases by stimulating cellular defense mechanisms.

Secoemestrin D, A Cytotoxic Epitetrathiodioxopiperizine, And Emericellenes A E, Five Sesterterpenoids From Emericella Sp. Ast0036, A Fungal Endophyte Of Astragalus Lentiginosus1. Source: Journal Of Natural Products
November 19th, 2013 PMID: 24251417 Leslie Gunatilaka
A new epitetrathiodioxopiperizine, secoemestrin D (1), and five sesterterpenoids bearing a new carbon skeleton, emericellenes A-E (2-6), together with previously known fungal metabolites, sterigmatocystin (7), arugosin C (8), and epiisoshamixanthone (9), were obtained from the endophytic fungal strain Emericella sp. AST0036 isolated from a healthy leaf tissue of Astragalus lentiginosus. The planar structures and relative configurations of the new metabolites 1-6 were elucidated using MS and 1D and 2D NMR spectroscopic data. All compounds were evaluated for their potential anticancer activity using a panel of six tumor cell lines and normal human fibroblast cells. Only metabolites 1 and 7 showed cytotoxic activity. More importantly, secoemestrin D (1) exhibited significant cytotoxicity with IC50 values ranging from 0.06 to 0.24 μM and moderate selectivity to human glioma (SF-268) and metastatic breast adenocarcinoma (MDA-MB-231) cell lines.

Phomapyrrolidones A C, Antitubercular Alkaloids From The Endophytic Fungus Phoma Sp. Nrrl 46751. Source: Journal Of Natural Products
September 30th, 2013 PMID: 24079882 Leslie Gunatilaka
Three new alkaloids, phomapyrrolidones A-C (1-3), bearing a cyclopenta[b]fluorene ring system were isolated from the mycelium extract of the endophytic fungal strain Phoma sp. NRRL 46751, inhabiting Saurauia scaberrinae. Methylation of 1 afforded its N-methyl derivative 4. The planar structures and relative configurations of 1-4 were elucidated by extensive spectroscopic analysis. Phomapyrrolidones B (2) and C (3) exhibited weak antitubercular activity at subcytotoxic concentrations.

Endohyphal Bacterium Enhances Production Of Indole 3 Acetic Acid By A Foliar Fungal Endophyte. Source: Plo S One
September 24th, 2013 PMID: 24086270 Leslie Gunatilaka
Numerous plant pathogens, rhizosphere symbionts, and endophytic bacteria and yeasts produce the important phytohormone indole-3-acetic acid (IAA), often with profound effects on host plants. However, to date IAA production has not been documented among foliar endophytes -- the diverse guild of primarily filamentous Ascomycota that live within healthy, above-ground tissues of all plant species studied thus far. Recently bacteria that live within hyphae of endophytes (endohyphal bacteria) have been detected, but their effects have not been studied previously. Here we show not only that IAA is produced in vitro by a foliar endophyte (here identified as Pestalotiopsis aff. neglecta, Xylariales), but that IAA production is enhanced significantly when the endophyte hosts an endohyphal bacterium (here identified as Luteibacter sp., Xanthomonadales). Both the endophyte and the endophyte/bacterium complex appear to rely on an L-tryptophan dependent pathway for IAA synthesis. The bacterium can be isolated from the fungus when the symbiotic complex is cultivated at 36°C. In pure culture the bacterium does not produce IAA. Culture filtrate from the endophyte-bacterium complex significantly enhances growth of tomato in vitro relative to controls and to filtrate from the endophyte alone. Together these results speak to a facultative symbiosis between an endophyte and endohyphal bacterium that strongly influences IAA production, providing a new framework in which to explore endophyte-plant interactions.

Structure Activity Relationship (Sar) Of Withanolides To Inhibit Hsp90 For Its Activity In Pancreatic Cancer Cells. Source: Investigational New Drugs
July 26th, 2013 PMID: 23887853 Leslie Gunatilaka
Withaferin A (WA), a naturally occurring steroidal lactone, directly binds to Hsp90 and leads to the degradation of Hsp90 client protein. The purpose of this study is to investigate the structure activity relationship (SAR) of withanolides for their inhibition of Hsp90 and anti-proliferative activities in pancreatic cancer cells. In pancreatic cancer Panc-1 cells, withaferin A (WA) and its four analogues withanolide E (WE), 4-hydroxywithanolide E (HWE), 3-aziridinylwithaferin A (AzWA) inhibited cell proliferation with IC50 ranged from 1.0 to 2.8 μM. WA, WE, HWE, and AzWA also induced caspase-3 activity by 21-, 6-, 11- and 15-fold, respectively, in Panc-1 cells, while withaperuvin (WP) did not show any activity. Our data showed that WA, WE, HWE, and AzWA, but not WP, all directly bound to Hsp90 and induced Hsp90 aggregation,hence inhibited Hsp90 chaperone activity to induce degradation of Hsp90 client proteins Akt and Cdk4 through proteasome-dependent pathway in pancreatic cancer cells. However, only WA, HWE and AzWA disrupted Hsp90-Cdc37 complexes but not WE and WP. SAR study suggested that the C-5(6)-epoxy functional group contributes considerably for withanolide to bind to Hsp90, inhibit Hsp90 chaperone activity, and result in Hsp90 client protein depletion. Meanwhile, the hydroxyl group at C-4 of ring A may enhance withanolide to inhibit Hsp90 activity and disrupt Hsp90-Cdc37 interaction. These SAR data provide possible mechanisms of anti-proliferative action of withanolides.

Synthesis And Biological Evaluation Of Novobiocin Analogues As Potential Heat Shock Protein 90 Inhibitors. Source: Bioorganic & Medicinal Chemistry
June 27th, 2013 PMID: 23859777 Leslie Gunatilaka
Recent studies have shown that novobiocin (NB), a member of the coumermycin (CA) family of antibiotics with demonstrated DNA gyrase inhibitory activity, inhibits Heat shock protein 90 (HSP90) by binding weakly to a putative ATP-binding site within its C-terminus. To develop more potent HSP90 inhibitors that target this site and to define structure-activity relationships (SARs) for this class of compounds, we have synthesized twenty seven 3-amido-7-noviosylcoumarin analogues starting from NB and CA. These were evaluated for evidence of HSP90 inhibition using several biological assays including inhibition of cell proliferation and cell cycle arrest, induction of the heat shock response, inhibition of luciferase-refolding in vitro, and depletion of the HSP90 client protein c-erbB-2/HER-2/neu (HER2). This SAR study revealed that a substantial increase in biological activity can be achieved by introduction of an indole-2-carboxamide group in place of 4-hydroxy-isopentylbenzamido group at C-3 of NB in addition to removal/derivatization of the 4-hydroxyl group from the coumarin ring. Methylation of the 4-hydroxyl group in the coumarin moiety moderately increased biological activity as shown by compounds 11 and 13. Our most potent new analogue 19 demonstrated biological activities consistent with known HSP90-binding agents, but with greater potency than NB.

Geopyxins A E, Ent Kaurane Diterpenoids From Endolichenic Fungal Strains Geopyxis Aff. Majalis And Geopyxis Sp. Az0066: Structure Activity Relationships Of Geopyxins And Their Analogues. Source: Journal Of Natural Products
January 20th, 2012 PMID: 22264149 Leslie Gunatilaka
Four new ent-kaurane diterpenoids, geopyxins A-D (1-4), were isolated from Geopyxis aff. majalis, a fungus occurring in the lichen Pseudevernia intensa, whereas Geopyxis sp. AZ0066 inhabiting the same host afforded two new ent-kaurane diterpenoids, geopyxins E and F (5 and 6), together with 1 and 3. The structures of 1-6 were established on the basis of their spectroscopic data, while the absolute configurations were assigned using modified Mosher's ester method. Methylation of 1-3, 5, and 6 gave their corresponding methyl esters 7-11. On acetylation, 1 and 7 yielded their corresponding monoacetates 12 and 14 and diacetates 13 and 15. All compounds were evaluated for their cytotoxic and heat-shock induction activities. Compounds 2, 7-10, 12, 14, and 15 showed cytotoxic activity in the low micromolar range against all five cancer cell lines tested, but only compounds 7-9, 14, and 15 were found to activate the heat-shock response at similar concentrations. From a preliminary structure-activity perspective, the electrophilic α,β-unsaturated ketone carbonyl motif present in all compounds except 6 and 11 was found to be necessary but not sufficient for both cytotoxicity and heat-shock activation.

Using The Heat Shock Response To Discover Anticancer Compounds That Target Protein Homeostasis. Source: Acs Chemical Biology
November 30th, 2011 PMID: 22050377 Leslie Gunatilaka
Unlike normal tissues, cancers experience profound alterations in protein homeostasis. Powerful innate adaptive mechanisms, especially the transcriptional response regulated by Heat Shock Factor 1 (HSF1), are activated in cancers to enable survival under these stressful conditions. Natural products that further tax these stress responses can overwhelm the ability to cope and could provide leads for the development of new, broadly effective anticancer drugs. To identify compounds that drive the HSF1-dependent stress response, we evaluated over 80,000 natural and synthetic compounds as well as partially purified natural product extracts using a reporter cell line optimized for high-throughput screening. Surprisingly, many of the strongly active compounds identified were natural products representing five diverse chemical classes (limonoids, curvularins, withanolides, celastraloids, and colletofragarones). All of these compounds share the same chemical motif, an α,β-unsaturated carbonyl functionality, with strong potential for thiol-reactivity. Despite the lack of a priori mechanistic requirements in our primary phenotypic screen, this motif was found to be necessary albeit not sufficient, for both heat-shock activation and inhibition of glioma tumor cell growth. Within the withanolide class, a promising therapeutic index for the compound withaferin A was demonstrated in vivo using a stringent orthotopic human glioma xenograft model in mice. Our findings reveal that diverse organisms elaborate structurally complex thiol-reactive metabolites that act on the stress responses of heterologous organisms including humans. From a chemical biology perspective, they define a robust approach for discovering candidate compounds that target the malignant phenotype by disrupting protein homeostasis.

An Analog Of Withaferin A Activates The Mapk And Glutathione "Stress" Pathways And Inhibits Pancreatic Cancer Cell Proliferation. Source: Cancer Investigation
November 17th, 2011 PMID: 22085270 Leslie Gunatilaka
Withaferin A (WA) (1) and two analogs [4-epi-withaferin A (2) and 4,27-diacetyl-4-epi-withaferin A (3)] were evaluated for antitumor activity in pancreatic cancer cells. IC(50) for 1, 2, and 3 were 0.87, 0.45, and 0.29 ?M (BxPC-3); 1.28, 1.53, and 0.52 ?M (MIAPaCa-2); and 0.59, 2.25, and 0.56 ?M (PANC-1), respectively. We chose WA analog 3 for functional studies with confirmatory RT-PCR and Western blotting. ANOVA identified 33 (MIAPaCa-2), 54 (PANC-1), and 48 (BxPC-3) gene expression changes. Fisher exact test demonstrated MAPK and glutathione pathways to be overexpressed with WA analog 3. WA analog 3 elicits a dose- and time-dependent apoptosis, activates MAPK and glutathione ?stress? pathways, and inhibits proliferation.

Smardaesidins A G, Isopimarane And 20 Nor Isopimarane Diterpenoids From Smardaea Sp., A Fungal Endophyte Of The Moss Ceratodon Purpureus. Source: Journal Of Natural Products
October 14th, 2011 PMID: 21999655 Leslie Gunatilaka
Five new isopimarane diterpenes, smardaesidins A-E (1- 5) and two new 20-nor-isopimarane diterpenes, smardaesidins F (6) and G (7), together with sphaeropsidins A (8) and C-F (10-13) were isolated from an endophytic fungal strain, Smardaea sp. AZ0432, occurring in living photosynthetic tissue of the moss Ceratodon purpureus . Of these, smardaesidins B (2) and C (3) were obtained as an inseparable mixture of isomers. Chemical reduction of sphaeropsidin A (8) afforded sphaeropsidin B (9), whereas catalytic hydrogenation of 8 yielded 7-O-15,16-tetrahydrosphaeropsidin A (14) and its new derivative, 7-hydroxy-6-oxoisopimara-7-en-20-oic acid (15). The acetylation and diazomethane reaction of sphaeropsidin A (8) afforded two of its known derivatives, 6-O-acetylsphaeropsidin A (16) and 8,14-methylenesphaeropsidin A methyl ester (17), respectively. Methylation of 10 yielded sphaeropsidin C methyl ester (18). The planar structures and relative configurations of the new compounds 1-7 and 15 were elucidated using MS and 1D and 2D NMR experiments, while the absolute configurations of the stereocenters of 4 and 6-8 were assigned using a modified Mosher's ester method, CD spectra, and comparison of specific rotation data with literature values. Compounds 1-18 were evaluated for their potential anticancer activity using several cancer cell lines and cells derived from normal human primary fibroblasts. Of these, compounds 8, 11, and 16 showed significant cytotoxic activity. More importantly, sphaeropsidin A (8) showed cell-type selectivity in the cytotoxicity assay and inhibited migration of metastatic breast adenocarcinoma (MDA-MB-231) cells at subcytotoxic concentrations.

Unusual Withanolides From Aeroponically Grown Withania Somnifera. Source: Phytochemistry
March 22nd, 2011 PMID: 21315384 Leslie Gunatilaka
In an attempt to maximize production and the structural diversity of plant metabolites, the effect of growing the medicinal plant Withania somnifera under soil-less aeroponic conditions on its ability to produce withaferin A and withanolides was investigated. It resulted in the isolation and characterization of two compounds, 3α-(uracil-1-yl)-2,3-dihydrowithaferin A (1) and 3β-(adenin-9-yl)-2,3-dihydrowithaferin A (2), in addition to 10 known withanolides including 2,3-dihydrowithaferin A-3β-O-sulfate. 3β-O-Butyl-2,3-dihydrowithaferin A (3), presumably an artifact formed from withaferin A during the isolation process was also encountered. Reaction of withaferin A with uracil afforded 1 and its epimer, 3β-(uracil-1-yl)-2,3-dihydrowithaferin A (4). The structures of these compounds were elucidated on the basis of their high resolution mass and NMR spectroscopic data.

Biomimetic Conversion Of ( ) Fusoxypyridone And ( ) Oxysporidinone To ( ) Sambutoxin: Further Evidence For The Structure Of The Tricyclic Pyridone Alkaloid, ( ) Fusoxypyridone. Source: Bioorganic & Medicinal Chemistry Letters
March 16th, 2011 PMID: 21419624 Leslie Gunatilaka
Biomimetic-type reactions of the tricyclic pyridone alkaloid, (-)-fusoxypyridone [(-)-4,6'-anhydrooxysporidinone] (1), recently encountered in an endophytic strain of Fusarium oxysporum, and (-)-oxysporidinone (2) afforded (-)-sambutoxin (3) and an analogue of 1, identified as (-)-1'(6')-dehydro-4,6'-anhydrooxysporidinone (4), thus confirming the structure previously proposed for 1 and suggesting that 1-3 bear the same relative stereochemistry. Oxidation of 4 with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) yielded a hitherto unknown sambutoxin analogue, (-)-4,2'-anhydrosambutoxin (5).

Microbial Transformations Of Aryltetralone And Aryltetralin Lignans By Cunninghamella Echinulata And Beauveria Bassiana. Source: Journal Of Natural Products
October 20th, 2010 PMID: 20961092 Leslie Gunatilaka
Microbiological transformation of the aryltetralone lignan (-)-8'-epi-aristoligone (1) with Cunninghamella echinulata ATCC 10028B afforded two known natural lignans, (-)-holostyligone (3) and (-)-arisantetralone (4). Incubation of the aryltetralin lignan (-)-isogalbulin (2), obtained by chemical transformation of 1, with C. echinulata ATCC 10028B afforded the known lignan aryltetralol (5) and seven new metabolites, (-)-8-hydroxyisogalbulin (6), (-)-7-methoxyisogalbulin (7), (-)-4'-O-demethyl-8-hydroxyisogalbulin (8), (-)-7-methoxy-8-hydroxyisogalbulin (9), (-)-4'-O-demethyl-7-methoxyisogalbulin (10), (-)-4',5-O-didemethylcyclogalgravin (11), and (-)-4'-O-demethylcyclogalgravin (12). When 2 was subjected to biotransformation with Beauveria bassiana ATCC 7159, (-)-8-hydroxyisogalbulin (6) was the only isolable product. The structures of all new compounds were established by detailed analysis of their spectroscopic data.

Pkc Signaling Regulates Drug Resistance Of The Fungal Pathogen Candida Albicans Via Circuitry Comprised Of Mkc1, Calcineurin, And Hsp90. Source: P Lo S Pathogens
August 26th, 2010 PMID: 20865172 Leslie Gunatilaka
Fungal pathogens exploit diverse mechanisms to survive exposure to antifungal drugs. This poses concern given the limited number of clinically useful antifungals and the growing population of immunocompromised individuals vulnerable to life-threatening fungal infection. To identify molecules that abrogate resistance to the most widely deployed class of antifungals, the azoles, we conducted a screen of 1,280 pharmacologically active compounds. Three out of seven hits that abolished azole resistance of a resistant mutant of the model yeast Saccharomyces cerevisiae and a clinical isolate of the leading human fungal pathogen Candida albicans were inhibitors of protein kinase C (PKC), which regulates cell wall integrity during growth, morphogenesis, and response to cell wall stress. Pharmacological or genetic impairment of Pkc1 conferred hypersensitivity to multiple drugs that target synthesis of the key cell membrane sterol ergosterol, including azoles, allylamines, and morpholines. Pkc1 enabled survival of cell membrane stress at least in part via the mitogen activated protein kinase (MAPK) cascade in both species, though through distinct downstream effectors. Strikingly, inhibition of Pkc1 phenocopied inhibition of the molecular chaperone Hsp90 or its client protein calcineurin. PKC signaling was required for calcineurin activation in response to drug exposure in S. cerevisiae. In contrast, Pkc1 and calcineurin independently regulate drug resistance via a common target in C. albicans. We identified an additional level of regulatory control in the C. albicans circuitry linking PKC signaling, Hsp90, and calcineurin as genetic reduction of Hsp90 led to depletion of the terminal MAPK, Mkc1. Deletion of C. albicans PKC1 rendered fungistatic ergosterol biosynthesis inhibitors fungicidal and attenuated virulence in a murine model of systemic candidiasis. This work establishes a new role for PKC signaling in drug resistance, novel circuitry through which Hsp90 regulates drug resistance, and that targeting stress response signaling provides a promising strategy for treating life-threatening fungal infections.

Maximizing Chemical Diversity Of Fungal Metabolites: Biogenetically Related Heptaketides Of The Endolichenic Fungus Corynespora Sp. (1). Source: Journal Of Natural Products
June 25th, 2010 PMID: 20521776 Leslie Gunatilaka
In an attempt to explore the biosynthetic potential of the endolichenic fungus Corynespora sp. BA-10763, its metabolite profiles under several culture conditions were investigated. When cultured in potato dextrose agar, it produced three new heptaketides, 9-O-methylscytalol A (1), 7-desmethylherbarin (2), and 8-hydroxyherbarin (3), together with biogenetically related metabolites scytalol A (4), 8-O-methylfusarubin (5), scorpinone (6), and 8-O-methylbostrycoidin (7), which are new to this organism, and herbarin (8), a metabolite previously encountered in this fungal strain. The use of malt extract agar as the culture medium led to the isolation of 6, 8, 1-hydroxydehydroherbarin (9), and 1-methoxydehydroherbarin (10), which was found to be an artifact formed during the extraction of the culture medium with methanol. The structures of all new compounds were determined by interpretation of their spectroscopic data and chemical interconversions.

Tricinonoic Acid And Tricindiol, Two New Irregular Sesquiterpenes From An Endophytic Strain Of Fusarium Tricinctum. Source: Natural Product Research
March 11th, 2010 PMID: 20221941 Leslie Gunatilaka
Two new rare irregular sesquiterpenes, tricinonoic acid (1) and tricindiol (2), and the known furanopyrrolidones, NG-391 (3) and NG-393 (4), have been isolated from an EtOAc extract of Fusarium tricinctum, a fungus endophytic in the root tissue of the Sonoran desert plant, Rumex hymenosepalus. The structures of 1 and 2 were elucidated on the basis of their high-resolution mass, 1D and 2D NMR spectroscopic data. A possible biosynthetic route to 1 and 2 from farnesyl diphosphate is proposed.

Withaferin A Targets Heat Shock Protein 90 In Pancreatic Cancer Cells. Source: Biochemical Pharmacology
December 16th, 2009 PMID: 19769945 Leslie Gunatilaka
The purpose of this study is to investigate the efficacy and the mechanism of Hsp90 inhibition of Withaferin A (WA), a steroidal lactone occurring in Withania somnifera, in pancreatic cancer in vitro and in vivo. Withaferin A exhibited potent antiproliferative activity against pancreatic cancer cells in vitro (with IC(50)s of 1.24, 2.93 and 2.78 microM) in pancreatic cancer cell lines Panc-1, MiaPaCa2 and BxPc3, respectively. Annexin V staining showed that WA induced significant apoptosis in Panc-1 cells in a dose-dependent manner. Western blotting demonstrated that WA inhibited Hsp90 chaperone activity to induce degradation of Hsp90 client proteins (Akt, Cdk4 and glucocorticoid receptor), which was reversed by the proteasomal inhibitor, MG132. WA-biotin pull down assay of Hsp90 using Panc-1 cancer cell lysates and purified Hsp90 showed that WA-biotin binds to C-terminus of Hsp90 which was competitively blocked by unlabeled WA. Co-immunoprecipitation exhibited that WA (10 microM) disrupted Hsp90-Cdc37 complexes from 1 to 24h post-treatment, while it neither blocked ATP binding to Hsp90, nor changed Hsp90-P23 association. WA (3, 6mg/kg) inhibited tumor growth in pancreatic Panc-1 xenografts by 30% and 58%, respectively. These data demonstrate that Withaferin A binds Hsp90, inhibits Hsp90 chaperone activity through an ATP-independent mechanism, results in Hsp90 client protein degradation, and exhibits in vivo anticancer activity against pancreatic cancer.