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Bill Rancic Defends His Wife Giuliana After Fashion Police Controversy: 'I Tried to Get Them to Release the Footage' 42 years, 2,191 covers and 55,436 stories from PEOPLE magazine's history for you to enjoy
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- December 29, 1997
- Vol. 48
- No. 26
John F. Kennedy Jr.
Successful and Settled, He Cones into His Own
The odd juxtaposition of vim and virtue seems to sum up the two sides of the 36-year-old Kennedy's personality. He may have ended his giddy run as America's most eligible bachelor, but the John-John we knew and loved—unassuming, outdoorsy, fun-loving—is still kicking. At the same time, he has emerged as a sober, canny businessman, assuming control of his two-year-old political magazine. "He fooled everyone," says Kennedy family intimate, architect Richard Meier. "He said, 'Hey, I'm a serious guy, and I'm going to have a serious life.' " In the process, says author John H. Davis (Jacqueline Bouvier: An Intimate Memoir), "he proclaimed himself the leader of the [younger] clan."
At home, John and wife Carolyn, 31, keep a low profile, ducking out of their downtown Manhattan apartment for pizza despite the constant paparazzi presence. When he appeared with a bandaged hand last October, rumors flew that the couple were fighting, but the wound was blamed on a kitchen accident. "They're very happy," says Meier. Now, with his personal and professional life on track, Kennedy may be looking for new challenges. Former JFK press secretary Pierre Salinger, for one, believes, "In the next century, John will go into politics."
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Preliminary results of parts of this study were presented at the 19th Annual Meeting of the Society for Neuroscience and the 33rd Annual Meeting of the Society of Toxicology and were published in abstract form in Soc. Neurosci. Abstr. (1993) 19, 1749, and The Toxicologist (1994) 14, 290. This work was submitted by M.F.D. in partial completion of the requirements of the Ph.D. degree in Pharmacology and Toxicology and Environmental Toxicology at Michigan State University.
Methylmercury-Induced Elevations in Intrasynaptosomal Zinc Concentrations: An 19F-NMR Study†
Version of Record online: 23 NOV 2002
DOI: 10.1046/j.1471-4159.1994.63010383.x
Journal of Neurochemistry
Volume 63, Issue 1, pages 383–386, July 1994
Additional Information
How to Cite
Denny, M. F. and Atchison, W. D. (1994), Methylmercury-Induced Elevations in Intrasynaptosomal Zinc Concentrations: An 19F-NMR Study. Journal of Neurochemistry, 63: 383–386. doi: 10.1046/j.1471-4159.1994.63010383.x
- †
Publication History
- Issue online: 23 NOV 2002
- Version of Record online: 23 NOV 2002
- Resubmitted manuscript received April 5, 1994; accepted April 11, 1994.
- Zn2+;
- Methylmercury;
- Synaptosomes;
- Neurotoxicity;
- Heavy metal
Abstract: Methylmercury (MeHg) increases the concentration of intracellular Ca2+ ([Ca2+]i) and another endogenous polyvalent cation in both synaptosomes and NG108-15 cells. In synaptosomes, the elevation in [Ca2+]i was strictly dependent on extracellular Ca2+ (Ca2+e); similarly, in NG108-15 cells, a component of the elevations in [Ca2+]i was Ca2+e dependent. The MeHg-induced elevations in endogenous polyvalent cation concentration were independent of Ca2+e in synaptosomes and NG108-15 cells. The pattern of alterations in fura-2 fluorescence suggested the endogenous polyvalent cation may be Zn2+. Using 19F-NMR spectroscopy of rat cortical synaptosomes loaded with the fluorinated chelator 1,2-bis(2-amino-5-fluorophenoxy)ethane-N,N,N′,N′-tetraacetic acid (5F-BAPTA), we have determined unambiguously that MeHg increases the free intrasynaptosomal Zn2+ concentration ([Zn2+]i). In buffer containing 200 µM EGTA to prevent the Ca2+e-dependent elevations in [Ca2+]i, the [Zn2+]i was 1.37 ± 0.20 nM; following a 40-min exposure to MeHg-free buffer [Zn2+]i was 1.88 ± 0.53 nM. Treatment of synaptosomes for 40 min with 125 µM MeHg yielded [Zn2+]i of 2.69 ± 0.55 nM, whereas 250 µM MeHg significantly elevated [Zn2+]i to 3.99 ± 0.68 nM. No Zn2+ peak was observed in synaptosomes treated with the cell-permeant heavy metal chelator N,N,N′,N′-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN, 100 µM) following 250 µM MeHg exposure. [Ca2+]i in buffer containing 200 µM EGTA was 338 ± 26 nM and was 370 ± 64 nM following an additional 40-min exposure to MeHg-free buffer. [Ca2+]i was 498 ± 28 or 492 ± 53 nM during a 40-min exposure to 125 or 250 µM MeHg, respectively. None of the values of [Ca2+]i differed significantly from either pretreatment levels or buffer-treated controls.

subset: commoncrawl


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Overview and Guidelines
Overview and Guidelines
- "What Every New Screener Should Know" Introduction to the Screening Process
- Initiating a Screening Project at ICCB-Longwood
- Biosafety Requirements
- Designing a High Throughput Assay
- Performing a Screen
- Data Handling
- Small Molecule Compounds for Secondary Screens
- Medicinal Chemistry Resources
- RNAi Reagents for Secondary Screens
- Other Questions?
"What Every New Screener Should Know" Introduction to the Screening Process
Click here for a link to view or download the 'What Every New Screener Should Know' document, which is an important introduction to screening at the ICCB-Longwood facility. This is a quick reference guide that is distinct from the additional information below.
Initiating a Screening Project at ICCB-Longwood
The ICCB-Longwood Screening Facility collaborates with academic investigators to perform high-throughput screens of chemical and RNAi libraries. Our compound collection includes over 500,000 small molecules, including commercially available compound libraries and unique natural product libraries. We also offer genome-scale RNAi screening. Dharmacon SMARTpool siRNA libraries targeting the whole human genome (18,220 genes), the whole mouse genome (20,552 genes) and 3 different human miRNA collections are available for screening at ICCB-L. Data generated from screens are entered into a screening results database. Access to this non-public database (Screensaver) is granted only to ICCB-Longwood investigators and collaborators who have deposited data. For more information on small molecule and RNAi data in the database, please see the ICCB-L small molecule user agreement and ICCB-L RNAi user agreement, both area available to download on the Screening Documents page.
ICCB-Longwood screening facility personnel assist efforts by providing access to the compound and RNAi libraries, and by operating and maintaining the screening robots. They also offer advice at all stages of the screening process, including assay design and optimization, screening, data analysis and follow-up approaches. Collaborators perform their own assays and provide their own supplies, including assay plates. A fee is charged for access to screening at ICCB-Longwood. Please see the small molecule application page and the RNAi application page for more details about the fee structures.
To allow us to prioritize new screening projects appropriately, a formal application must be submitted in order to initiate a screen at ICCB-Longwood. Additional applications must be submitted each time a new screen is proposed. We currently review applications on a rolling basis (links to the applications appear in the previous paragraph or on the left hand navigator bar under Application Forms).
To avoid situations in which two different groups are carrying out substantially similar screens on the same small molecule collections or RNAi libraries at the same time, we attempt not to initiate screens that compete with existing ones. Whether a proposed screen will compete with ongoing assays is decided by the ICCB-Longwood Screening Application Review Committee in consultation with the investigators involved. Precedence is generally given to the screen first proposed.
Radioactive assays are currently not supported in the ICCB-Longwood Screening Facility, and substances requiring a higher containment level than BSL2 are not permitted.
Please send questions about the application process to screeniccb_apply"AT"hms.harvard.edu.
After an application is accepted, the investigator is granted access to the screening facility. Click to link to the document "What Every New Screener Should Know", which is requisite reading before a screener's first visit to the ICCB-Longwood Screening Facility.
The maximum biocontainment level for studies conducted in the screening facility is BSL2.
Biosafety procedures for the screening facility are governed by Harvard Medical School regulations, overseen by the Committee on Microbiological Safety (COMS). The screening facility currently holds a biosafety approval covering a variety of organisms. There may be experimental design constraints for certain organisms that are considered highly infectious or that present an unusual health hazard.
Biosafety requirements are evaluated when an equipment training request is submitted. If you are wondering whether the organism(s) you would like to work with has been approved for use at ICCB-Longwood, please contact Jennifer Smith (jennifer_smith"at"hms.harvard.edu) or Katrina Rudnicki (katrina_rudnicki"at"hms.harvard.edu). If your screen involves items not approved, we can file an amendment to add your reagents to the list. To assist us in this process, if you are not a Harvard Medical School-affiliated researcher, please provide a copy of your institutional biosafety committee approval for all research procedures applicable to the screen. Depending on the format of your institutional approval, you may have to provide additional information describing your proposed work. For example, the COMS application requests names and descriptions of strains, plasmids, experimental design, and biosafety issues. Please review the HMS COMS application form to see what is required. Please be aware that COMS approval may take a month or more, so it is important to file amendments as early as possible.
Your BSL2, hazardous waste, and bloodborne pathogen training should also be completed and up-to-date. Annual refresher courses for Harvard personnel are available online through Environmental Health and Safety.
For screeners not holding current Harvard IDs, access to the screening facility is contingent upon your institutional biosafety office co-signing the screening facility user agreement to indicate that your employer has provided all necessary BSL2, hazardous waste, and bloodborne pathogen training and protective equipment. For practical purposes, however, site-specific training and protective equipment will be provided by the screening facility as necessary.
All ICCB-L screeners must complete the ICCB-L Screening Facility Lab Specific Training prior to beginning any work in the facility. This brief training reviews the location of emergency equipment and policies specific to the screening rooms. Please complete the online Training Request Form to get started. In addition, the ICCB-L Lab Safety Handbook can be viewed or printed here (PDF).
Designing a High Throughput Assay
For a high-throughput screen, a biochemical or cell-based assay must be adapted to microtiter plate format. Assays are most commonly performed in 384-well assay plates; in certain cases, they may also be performed in 96-well or 1536-well plates. Assay volumes in 384-well plates range from 5 µL (in low-volume plates) to 100 µL (in standard plates). At the low end of this range, inaccuracies in small-volume pipetting can cause signal variation, while there is a risk of spillage and cross-contamination at the high end. We therefore recommend that, if possible, investigators use 30-50 µl assays in standard plates and avoid dispense volumes lower than 5 µL.
Different types of plates are recommended depending on the assay detection method used. White plates are recommended for luminescence assays, black plates for fluorescence assays, clear-bottom plates for automated microscopy, and clear polystyrene plates for photometric assays. Please see the Screening Supplies section for standard plate options.
Most high-throughput assays show a high amount of inherent variability and error. Therefore, it is strongly recommended that small molecule assays be performed in duplicate and RNAi assays be performed in triplicate. Using replicate data points can reduce false positive rates by up to one-half.
Control readings are essential to a well-designed assay, and every assay should use as many controls as possible. In general, there are two types of controls: plate-based controls and assay-wide controls. Plate-based controls are controls that are placed on each individual assay plate. These are essential for identifying plate-to-plate variability and establishing assay background levels. Assays that are prone to plate-based variability (such as luciferase readouts that decay over time) should primarily use plate-based controls and normalization. Stock library plates are formatted with empty wells to allow for screen-specific plate-based controls. It is good practice to use all available wells, with the researcher deciding on the appropriate distribution of positive and negative controls.
For small molecule screens, the ideal positive control is a small molecule that is known to produce the sought-after effect. Negative controls should match experimental conditions and only lack compound. For RNAi screens, the positive control should be an siRNA that knocks down a gene in the pathway of interest, producing the desired phonotype, while the negative control should be an identified non-targeting siRNA.
Assay-wide controls are separate plates containing only control wells and no screening compounds. These are particularly useful for determining the background levels of an assay and should be used to help determine whether an assay has sufficient signal to be reliably detected.
Signal/background ratio (S/B) and well-to-well variability (CV) are important issues to consider. As assay variability increases, the S/B ratio must increase for the screen to be successful. We recommend using a positive control condition to determine the S/B ratio. To determine S/B, fill a plate with reagents using the same equipment to be used for the screen. Add several dilutions of the positive control to several wells, and determine whether the positive control can be reproducibly detected above the well-to-well variation. These data will provide an indication of the false-positive and false-negative rate of your assay.
Quantitative Assay Evaluation and Optimization
The Z´ factor calculation is useful during piloting for quality assessment of assay conditions (Zhang et. al. 1999, see below for full reference). An assay can be considered validated for high-throughput screening after independent experiments (for example, each experiment set up separately starting from scratch or carried out on different days) have been shown to result in reproducible and suitable Z´ factor values. Each experiment should be performed on at least one full 384-well plate on which ½ of the wells contain positive controls and ½ of the wells contain negative controls. This will produce a statistically significant data set for evaluation. The experimental conditions to be used during the screen, including use of robotics, should be mimicked to the greatest extent possible.
To quantitatively rank assay conditions, perform control experiments and calculate Z´ factors from the data collected:
SD + = positive control standard deviation
SD – = negative control standard deviation
Ave + = positive control average
Ave – = negative control average
For small molecule screening assays, the below Z' values indicate assay fitness:
1 > Z´ > 0.9 An extremely robust assay
0.9 > Z´ > 0.7 A robust assay
0.7 > Z´ > 0.5 Acceptable, but identification of positives will benefit significantly from any improvement
0.5 = Z´ The minimum recommend for high throughput small molecule screening
This table may differ slightly from published recommendations. However, it is based on the general experience of small molecule screeners at ICCB-L. We commonly observe that screening results rarely achieve the high quality levels seen during piloting using controls. Also, cell-based assays frequently cannot achieve as high a Z' factor as assays using purified proteins.
For RNAi screening assays, the experience of ICCB-L screeners has been that Z' factor values under the most optimized conditions are often less than 0.5. These RNAi screening assays have nonetheless been productive.
Please contact us at email@example.com if you require more information.
Z'-factor reference: Ji-Hu Zhang, Thomas D. Y. Chung and Kevin R. Oldenburg (1999). A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen 4:67-73.
Once a satisfactory assay is developed in 384-well format, contact the ICCB-Longwood Screening Facility staff (iccb_screen"AT"hms.harvard.edu or 617-432-5815) to schedule your screen. Please contact the screening personnel well in advance of the date you would like to start your screen, as the screening schedule may be filled weeks in advance.
After establishing an assay protocol with an acceptable Z' factor, please schedule a meeting with the data curator and data analyst to discuss data handling. This can be done by emailing david_wrobel"AT"hms.harvard.edu and jennifer_nale"AT"hms.harvard.edu.
Screening facility personnel will provide screeners with training in the use of automated liquid handlers, plate readers, and screening microscopes. Only screening facility personnel are permitted to handle library stock plates, and thus they perform all transfers from library plates into assay plates. Screening facility personnel are not responsible for conducting any other parts of a screen.
Small molecule screens: we suggest you perform an initial pilot screen of 2,000 to 3,000 wells. Additional information about libraries available for screening is available at Compound Libraries. Note that we provide an up-to-date list of recommendations (link to PDF document in first paragraph) for the order in which to screen our many small molecule libraries. If the results of your pilot screen are satisfactory, you may continue to screen additional library plates. At this point, you must submit an assay protocol for inclusion in our screening database.
When performing a screen, it is preferable to process as many plates as possible during a single visit to the screening room. We strongly suggest screening all wells in duplicate to increase the significance of data points generated. The typical daily throughput for an individual small molecule screening project is approximately 20 plates in duplicate (40 plates total, or roughly 14,000 wells) for screens involving mammalian cells, and somewhat higher for microbial screens. An average ICCB-Longwood screening project assays a total of 50,000 to 100,000 compounds for biological activity.
RNAi Screens: For human siRNA screens, screeners must first screen the pilot plate, ICCBL Pilot1 (formerly piloting utilized Mitchison Kinase1). If the results of this pilot plate are satisfactory, screening of the Dharmacon libraries can be initiated. Mouse siRNA screens begin by screening plate 50,025, the first plate in the Dharmacon Mouse1: Kinase-Phosphatase Library.
For a variety of reasons (number of replicates, time sensitivity, complexity of steps, end-point readout), the RNAi screens do not proceed at as high of a throughput as small molecule screens. It is recommended that screeners build up to screening as many as 6 library plates at a time (with this number being dependent upon the number of replicates and conditions).
ICCB-Longwood provides screening libraries and access to equipment but does not provide supplies for screeners. Screeners are responsible for purchasing and shipping all applicable supplies to ICCB-Longwood for use in their screens.
Raw screening data should be saved directly to your ICCB-Longwood server account. Detailed instructions for data formatting and deposition into our screening results database may be downloaded as a PDF from the Screening Documents page. Once data are entered into the database, instruction will be provided as to how to view your data and compare your results to those of other screens.
Your data must be entered into the database in a timely manner after completing your primary screen. Selection of compounds/siRNAs for secondary assays ("cherry picking") is permitted only after deposition of initial data. Please see below for additional information on cherry picking.
While it is the responsibility of individual researchers to analyze their screen results, ICCB-Longwood staff can provide advice during this process. Some information may be viewed under the Data Analysis section: General Considerations and Interpretation of Hits.
Small Molecule Compounds for Secondary Screens
After completing a primary screen of a library, you should have many screening "positives" to follow-up in secondary screens. Subject to compound availability, we will provide you with approximately 1 µL of each selected screening positive compound (~5 µg). These are called "cherry picks". The maximum number of cherry picks allowed is equal to 0.3% of the number of compounds initially screened. For example, if the ChemBridge DiverSet E library is the initial screen completed (16,320 compounds), a maximum of 50 cherry picks can be requested. With adequate justification, exceptions to this policy might be made; Jennifer Smith (jennifer_smith"AT"hms.harvard.edu) for approval.
Screeners with a "hit" rate higher than 0.3% will often choose their cherry picks by examining the structures of their screening positives and comparing these data with others' screening data. This requires data entry into the Screensaver database and also time spent by the researcher mining the database. This step is usually important for the success of the project.
Requesting Small Molecule Cherry Picks:
Cherry-pick requests should be sent to David Wrobel (david_wrobel"AT"hms.harvard.edu) and are generally supplied within two weeks. Please view Instructions for requesting small molecule cherry picks for details about submitting a cherry pick request.
Please bear in mind that cherry picks are intended to help you obtain initial confirmation of results from your primary screen, and that we do not supply material for subsequent studies. For additional material, you must order it directly from a commercial source. Commercial vendors are often able to resupply a majority of their compounds for the first year after a library is purchased. The resupply rate varies unpredictably for older libraries. Please see the descriptions of the individual Compound Libraries for information on ordering.
For compounds no longer available from the original vendor, other supply sources may be found using public databases and other databases accessible via ICCB-Longwood. See the Interpretation of Hits section for information about these databases. Alternatively, custom synthesis can be arranged to obtain additional compound. Rates for custom synthesis vary considerably and must be negotiated directly with suppliers.
We strongly encourage the use of analytical chemistry methods such as HPLC to confirm the identity and purity of any material reordered for secondary studies.
Strategies for testing Small Molecule Cherry Picks:
As described below, there are three options for screeners to test their cherry picks, you can download this information as a PDF, located on the Screening Documents page.
Option 1 to test cherry picks: The screener may use the HP D300 liquid dispenser to retest the 1.0 uL cherry pick volume provided by the screening facility. The instrument uses proprietary HP T8 Dispensehead Cassettes available from Tecan. Each cassette allows for 8 locations for solutions to be dispensed into assay plate.
PLEASE NOTE that the only solvent supported at this time is 100% DMSO. Aqueous solutions cannot be used with the D300 at present.
For more information on the HP D300, please visit the Tecan website here.
Option 2 to test cherry picks: The screener may use essentially the same assay as for their primary screen, using PocketTips ® to transfer their cherry pick compounds into their assay plates instead of pin transfer. Use of PocketTips requires lab automation and assistance from the ICCB-L staff. PocketTips allow up to 4 transfers of 100 nL from each 1 µL sample of cherry picked compound at 5 mg/mL in DMSO, but compounds at higher concentrations of DMSO (e.g., Natural Product extracts) may get fewer transfers per 1 µL.
The ICCB-L PocketTips ® protocols require that cherry pick compounds (0.6-2 µL volume) are in ABgene AB-1056 microplates, and that the plates have been spun down in a centrifuge at 1000 rpm for 1 minute immediately prior to use. The destination assay microplate should contain 30 µL of media or assay buffer. A separate PocketTip will be used for each replicate. Please inquire with screening room staff about the current price of PocketTips. The PocketTip workflow is illustrated below.
PocketTips ® Workflow
Step 1 : Source Plate: Sample aspirated and pocket in tip fills to
capacity (50 nL, 100 nL, or 250 nL).
Step 2 : Source Plate: Sample dispensed back to cherry pick plate and pocket remains full.
Step 3 : Wash Plate: The portion of the tip below the pocket is washed to eliminate carryover.
Step 4 : Destination Plate: Sample/liquid in destination (assay) plate is aspirated to pocket level in the tip, and mixed.
Step 5 : Destination Plate: Mixed sample is dispensed back to the destination (assay) plate.
Option 3 to test cherry picks: The screener may take possession of the plate of cherry pick compounds from the screening room staff and use them for follow-up assays as they see fit. Until recently this was the only option available to screeners, and many screeners elect to dilute the cherry pick compounds in DMSO or buffer to "stretch" their cherry pick compounds for more assays. Please be aware of the issues involved with retesting compounds using a higher % of DMSO. If you have any questions about this, please contact a screening room staff member.
If you are interested in medicinal chemistry support for analysis of top screening hits or for compound follow up, please contact David Scott (davida_scott"AT"dfci.harvard.edu) and The Medicinal Chemistry Core at Dana-Farber Cancer Institute.
There are also other medicinal chemistry groups in the Boston area that have worked with ICCB-Longwood screeners in the past. For more information about these potential resources, please contact Jennifer Smith (jennifer_smith'AT'hms.harvard.edu)
RNAi Reagents for Secondary Screens
After completing a primary screen of one of Dharmacon's full genome siRNA libraries, there should be several "positives" to follow-up in secondary screens. We provide screeners with a "cherry pick" of the 4 individual duplexes targeting up to 300 genes (1200 duplexes total). Additional cherry picks can be requested at $1/duplex picked ($4/gene). Cherry picking will only be carried out for individual duplexes, not for SMARTpool reagents. Each duplex is provided at the same volume as was used when screening the SMARTpools in the primary screen. The purpose of this cherry pick is to enable screeners to perform a deconvolution screen, where the same assay is performed, but instead of using SMARTpools for knockdown, each of the 4 duplexes are tested individually.
A cherry pick may be requested at any point after screening of the Dharmacon libraries (Human 1-5, Mouse 1-5) has been initiated. Please note that it is not possible to cherry pick from the ICCBL Pilot1 plate. Cherry pick requests should be sent to David Wrobel (david_wrobel"AT"hms.harvard.edu) and are generally supplied within two weeks. Please view Instructions for requesting RNAi cherry picks (PDF) for details about submitting a cherry pick request.
If you require additional amounts of duplexes or SMARTpools, please refer to the vendor quotes on the ICCBL-L RNAi wiki for ordering information.
Please visit the FAQ section for answers to commonly asked screening questions, and click here to see information on how to build an academic high-throughput screening facility. If you have additional questions regarding the screening process or policies, contact the screening facility staff at iccb_screen"AT"hms.harvard.edu, Jennifer Smith (jennifer_smith"AT"hms.harvard.edu).
Please view "What Every New Screener Should Know", suggested reading before a screener's first visit to ICCB-Longwood.


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Ben Vereen Awards
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The Mummy Soldiers were displayed in Downtown San Diego in front of the Convention Center during Comic Con 2008.
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Shocking moment Australian woman jumped onto a moving train after platform argument, fell under it but SURVIVED
- Australian woman jumped onto freight train passing through station
- Slipped through gap in carriages but stood up when it passed
- Officers have blasted the incident at a New South Wales station as 'stupid'
- Believed she had stitches in head and back but discharged same day
This is the moment a woman fell under a moving train and survived.
The Australian woman was captured on CCTV sitting on the platform arguing with a man before hurling herself onto a freight train as it passed.
But losing her balance, she fell through the gap between carriages and lay motionless on the ground as the train, weighing thousands of pounds, ploughed over her.
Dangerous: The woman and man, sitting alone at 11pm, edge towards the moving train in New South Wales
Leap: Footage shows her stop smoking to launch herself onto an open space on the train
Shocking: The woman leaps onto a moving freight train as it rattles through Springwood Station in Australia
It powered through the Springwood Station, in the Blue Mountains of New South Wales, at 15-20mph.
The woman and man had been deep in discussion as it began to pass just after 11pm on December 28, 2013.
But suddenly she stands up, walks to the edge and - cigarette in hand - jumps on.
Incredibly, moments after the train had passed, the woman rolls over and stands up.
Lost balance: But as she jumps she loses her footing and tumbles towards the gap in the carriages
Lands: She falls onto the train, with her leg still visible and her friends still looking on calmly
Disappeared: The man visibly gasps as she disappears from sight and the 20kmph train powers on
Bleeding, she stumbles towards the edge and cannot muster the strength to pull herself up to safety.
Later footage, revealed today, shows the woman lying on the platform being treated by paramedics.
Viewers of the video - which has now gone viral - have questioned what the man was doing on the platform.
Officers have blasted the act.
Lucky to be alive: The woman lies motionless on the tracks as the train powers away
Stumble: She heaves herself, bleeding, to the edge but struggles to find the strength to lift herself up
Treated: Later the footage shows her being treated by paramedics. It is believed she received stitches in her head and back but was discharged from hospital the same day
'This girl has probably learned a lesson the hard way, she could have been killed or seriously injured,' Detective Inspector Mick Bostock told 7News.
'It's an offence for someone to touch a passing train or attempt to touch a passing train, so to actually try and jump on a moving goods train is very reckless and i wouldn't recommend it.'
It is believed she had stitches in her head and back before being discharged from hospital the same day.
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Management Information Systems Jobs in Cambridgeshire
Jobs 1 to 3 of 3
Software Developer EGA
You will implement scalable databases, ETL pipelines as well as java library and REST APIs for the management of genetic data and meta-data. The post will require the use of Confluence, Jira and Git in a team programming environment... You should hold a bachelors or higher degree in computer...
Hinxton near Cambridge, UK -
Salary: Competitive plus benefitsPosted: 2 days ago
Project Leader (Production)
Experience with bash scripting and advanced SQL querying. -Experience with scripting languages such as Perl/Python. -Experience with relational databases such as MySQL, PostgreSQL and/or Oracle... Git, subversion) and notions of Continuous Integration is important (e.g. Make, ant, Apache Maven, Apache Ivy, Jenkins and SonarQube, etc.) -Experience in...
Hinxton near Cambridge, UK -
Salary: Competitive plus benefitsPosted: 2 days ago
Scientific Programmer
A MSc in Computer Science, Bioinformatics or Cheminformatics (or equivalent); -Knowledge of cheminformatics/bioinformatics tools and resources (e.g., RDKit, ChemAxon, Pipeline Pilot, BLAST, UniProt, Protein Data Bank); -Experience working with Life Science or Drug Discovery data; -Experience in deploying and consuming RESTful web services; -Experience with continuous integration tools (...
Hinxton near Cambridge, UK -
Salary: Competitive plus benefitsPosted: 9 days ago
Management Information System Jobs in Cambridgeshire


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Senior Development Engineer, Electronics
Premium job
- Letchworth Garden City, Hertfordshire
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We are an innovative, robust and fast growing business, whose main focus is to deliver continues improvement to existing products and offer new sol..
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Smart Grid Research Engineer
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- Cumbernauld, Glasgow
- Grade: 6/7* £26,537 - £37,768*
Work as part of a growing dynamic team on a wide range of technical projects with particular emphasis on experimental validation and testing
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Electrical Asset Specialist
- Hatfield, Hertfordshire
Responsible for updating and writing electrical engineering standards, approved codes of practice and safe systems of work
- Recruiter: Affinity Water
Senior Electronics Engineer
- York, North Yorkshire
Senior electronics engineer to work as part of a team developing an MEG imaging system; working with the engineering team and external contractors.
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Manufacturing Engineer - Circuit Card Assembly
- Lostock Junction
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Whats the opportunity? Manufacturing UK is an integral part of the Operations Directorate whose principal mission is to ensure that MBDAs deliverable commitments are met...
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High Voltage Engineer
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This High Voltage Engineer will provide design leadership for high voltage cable assemblies up to one megavolt.
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Team Leader - Flank Arrays
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- Recruiter: BAE Systems
Electronics and Software Engineer
- Nottingham
- circa £35,000 per annum + bonus
Develop new test equipment for the pharmaceutical industry. Good opportunities to grow and develop. Successful family-owned and managed business.
- Recruiter: Copley Scientific Ltd
Bridge Test Facility Manager
- Shropshire, Telford, England
- Negotiable
Bridge Test Facility ManagerWe currently have a vacancy for a Bridge Test Facility Manager at our site in Telford with our Land UK business.As the Bridge Test Facility Manager, you will be part of our Test & Trials team, working closely with the Mili
- Recruiter: BAE Systems
Intelligent Transport Systems Engineer - Highways Technology
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Our transport technology team in Birmingham is currently growing a highly skilled and customer-focused team to...
- Recruiter: Mott MacDonald
Tiny fibres could have played role in 787 battery failure
Boeing 787 Dreamliner
The US National Transportation Safety Board is investigating whether tiny fibre-like formations, known as dendrites, inside lithium-ion batteries could have played a role in battery failures on two Boeing 787 Dreamliners last month.
Dendrites – just one of several possible causes under investigation by the agency – accumulate as a battery is charged and discharged and can cause short circuits, according to battery experts.
"As part of our continuing investigation, we are looking at whether dendrites may or may not have been a factor," Kelly Nantel, director of public affairs for the NTSB, said.
The Wall Street Journal reported this week that the NTSB was looking into dendrites, suggesting that investigators were looking at the tiny deposits as a major element in the probe.
Nantel said the NTSB has not ruled out any potential causes and that dendrites are "one of many things we are looking at" in determining what caused a battery aboard a parked Japan Airlines 787 to catch fire in Boston on 7 January.
"We are still considering several potential causes for the short circuiting" in the sixth of eight cells in the battery on the JAL plane, Nantel said.
NTSB chairman Deborah Hersman said last week that a short circuit in the lithium-ion battery had caused the fire.
The Japan Transport Safety Board (JTSB) is investigating a second 787 battery incident that prompted an All Nippon Airways plane to make an emergency landing in western Japan on 16 January. That battery showed signs of overheating.
Air safety regulators worldwide later grounded all 787s until the cause and a solution are found.
Boeing did not immediately respond to a request for comment.
The planemaker completed what it called an uneventful test flight of a 787 over the weekend, its first since the lightweight, carbon-composite aircraft was grounded.
Nantel said other factors under investigation include the state of charge of each cell and the method and delivery of that charge, contamination, electrode folds, wrinkles and pinches, "and the assembly of the cells and battery".
The NTSB is also looking at "the total design of the battery, including the physical separation of the cells, their electrical interconnections, and their thermal isolation from each other", she added.
Shares in GS Yuasa, a Japanese firm that makes batteries for the 787, slipped 0.3 per cent to 329 yen in Tokyo, underperforming a 2.4 per cent gain on the benchmark Nikkei.
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Check BNN and Bulbanews for up-to-date Pokémon news and discuss it on the forums or in our IRC channel #bulbagarden on irc.systemnet.info.
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Blastoise (Unleashed 13)
From Bulbapedia, the community-driven Pokémon encyclopedia.
Revision as of 04:49, 15 April 2013 by BulbaBot (Talk | contribs) (r2.7.3) (Robot: Adding zh:水箭龟(B))
Blastoise (Japanese: カメックス Kamex) is a Water-type Stage 2 Pokémon card. It is part of the Unleashed expansion.
Card text
Pokédex data
|Blastoise - Shellfish Pokémon
|5'03" (1.6 m)
|188.5 lbs. (85.5 kg)
|Pokédex entry
|It deliberately makes itself heavy so it can withstand the recoil of the water jets it fires.
|ふんしゃした みずの いきおいに まけないように わざと たいじゅうを おもくしているのだ。
Release information
Blastoise was the Prerelease promotional card for the Unleashed expansion. It was released in the Unleashed expansion, originating from the Japanese Battle Starter Decks. In March 2011, this card was also released in the Template:Merch and the Template:Merch as an exclusive Cracked Ice Holofoil.
This card's Pokédex entry comes from Pokémon HeartGold.
|This article is part of Project TCG, a Bulbapedia project that aims to report on every aspect of the Pokémon Trading Card Game.
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Bra-mazing! Amy Willerton sizzles in an updated version of Myleene Klass's famous white bikini on I'm A Celebrity
She's been showing off her enviable figure in a selection of itsy-bitsy bikinis throughout her stint on I'm A Celebrity this year.
And now Amy Willerton, 21, seems to have paid homage to former contestant, Myleene Klass, by wearing a white bikini just like the one she famously wore when she appeared on the show in 2006.
The star updated the number by wearing a swimsuit with a strap detail at the front in a look which is sure to garner her lots of attention.
Scroll down for video
Al-white! Amy poses in yet another bikini just like the one worn by Myleene Klass as she relaxes in the Australian jungle
The original: Myleene's white bikini moment back in 2006 has been mimicked time and time again
Amy spent the day topping up her tan on Wednesday night's episode of the show, as she relaxed in the sunshine in what is an emotional day for the Miss Great Britain sensation.
After waking up, she said: 'There is a strange vibe in camp this morning and there is a real "tensity" around,' making up her own word.
She added: 'I don't feel relaxed. I don't feel it's a happy environment and I don't think everybody is in the best of mood[s].'
Strapped up: The star adjusts her bikini as she prepares to dazzle in her outfit
It comes after Tuesday night's episode when Matthew Wright seemed to criticise Amy for relaxing too much and letting Lucy Pargeter do all the work around camp.
Earlier in the day, Amy asked professional dancer, Vincent Simone if she could have a sleep in his hammock shortly after waking up.
And Steve Davis said: 'Yes, it's been a little while since you've had a little sleep. About time you had another one.
'Do you remember those words you said on day one? You said to me you only need three hours sleep a night.'
Looking good: She surveys her assets as she gets changed in her jungle bed
But a sensitive Amy fought back: 'When I'm at home it is all I sleep, because I've been running around for so many months on just a few hours' sleep.
'When my body was given the chance to sleep it just went "boom"'.
'With all my running around I just get to do my emails at night and I end up staying up for hours and then getting up to go to the gym at 5am.'
'From who?' asked Lucy about her emails. 'Loads of different people, work opportunities, yeah.' said Amy.
Feeling sad: Amy was having an emotional day in the jungle despite her stunning appearance
'It's turned the other way round now,' said Steve, 'It would work like that for a couple of days, I'd agree with you, but I think you are basically going to be sleeping 24 hours stints.'
Steve said: 'She's probably doing 14 hours sleeps a day but it's not a problem, but we don't mind.'
Amy could sense Lucy wasn't feeling happy with her. In the Bush Telegraph Lucy explained:
'It does get to me that people feel that they can leave all responsibility in the outside world but we are a camp trying to survive.'
With that Amy decided to stay awake and not take her morning snooze.
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A Winthrop woman is asking for the public's assistance to find out who abandoned a dog in a ditch with its feet bound with Christmas garland. Cindy Edwards says she was on a walk around 4:30 p.m. Wednesday on State Route 11C when she came across the cocker spaniel laying on the side of the road in a ditch. See story.
View enlarged photo.
Massena village officials are looking at a "social host" law that would make it illegal for adults to host minors consuming illegal drugs and alcohol. The proposed law would punish adults with possible fines and jail time who "knowingly or unknowingly" allow people under age 21 to consume drugs on their property, according to the Massena Drug Free Coalition. An unlawfully dealing with a minor law already exists, but Massena Mayor Tim Currier says the law is "a lot more narrow."
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St. Lawrence County legislators would like the sheriff to take over the duty of issuing pistol permits. It's the latest in an ongoing effort from county legislators to end a long standing policy by County Court Judge Jerome Richards to put restrictions on nearly all of the conceal carry permits he approves.
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The Massena Artists Association recently hosted a juried art show with 94 works from 50 artists around the area. They will be on display at the Massena Public Library through June 3. Bill Parmer of Winthrop, above, won best in show. For more photos, click here.View enlarged photo.
Dozens of cars from the 1920s through the 21st century lined East Orvis Street in Massena for Frenchie's 'Great Northern New York Auto Show.' A portion of East Orvis Street was blocked for the event, which was also a fundraiser for the Boys and Girls Club of Massena. North Country Now photo by Andy Gardner. Go to http://bit.ly/20qZsR3 for more pictures from the event.View enlarged photo.
Volunteers at St. Mary's Cemetery apparently removed these memorial items from graves. Several people with family members buried there are upset over the action. Photo from Susan Barrett. See story.View enlarged photo.
What photographer Natalie Aldrich identifies as a coyote takes a peek at her as she photographs the beast. 'Ever feel like someone or something is watching you? Well, this coyote was sitting up when I first spotted him on the Brewer Road, Town of Canton. When he spotted me he laid down in the grass with just his head showing,' Aldrich wrote.View enlarged photo.
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At a House Space Subcommittee meeting on Capitol Hill last week, several companies laid out plans for deep space exploration. Lockheed Martin Vice President Wanda A. Sigur discussed the Orion Multi-Purpose Crew Vehicle the company is building for NASA, proposed cis-lunar space operations, and a Mars base camp orbiting the Red Planet. link.
Orbital ATK has unveiled preliminary plans to place a four-person habitat in cislunar space by 2020. The announcement during testimony to the U.S. House of Representatives Subcommittee on Space would not only see the creation of a permanent...Show More Summary
NASA and European Space Agency officials on May 19 said the European Service Module (ESM) for NASA's Orion crew-transport vehicle was within budget and on schedule for a late-2018 inaugural flight pending a key review.But the ESM program...Show More Summary
chrisozer: Monterey Bay Aquarium – April, 2016
One thing I've learned from the Okeanos Explorer's ROV trip to the Marianas Trench, is that deep sea jellyfish just like to let it all hang out. How else you going to feed effectively if you don't wave your tentacles around like you just don't care? But get too close to this unidentified jelly, and you risk triggering […]
The House Appropriations Committee has released a spending bill that would give NASA a budget of $19.5 billion for fiscal year 2017, which is $500 million above President Barack Obama's request. The measure boosts spending for exploration and science programs. link.more.
The U.S. Air Force relies on more than 5,000 aircraft to give it unmatched dominance over every other competitor on earth. The U.S. Navy, for its part, counts on more than 3,700 aircraft and 273 deployable battle force ships, which constitute...Show More Summary
General Atomics (GA) told IHS Jane's that it will have committed significant company funds to develop its 10 megajoule (MJ) Multimission Medium Range Railgun Weapon System (MMRRWS) from 2007 to the end of testing in 2017.Speaking toShow More Summary
Antibiotic-treated cows are bad news for climate and possibly ecosystems, because the drugs play havoc with microbes living inside dung beetles
Hawaii's endangered hawksbill sea turtles have been protected under the Endangered Species Act since 1978. So why are their population numbers still so low? Catch up on the latest research from Dr. Kyle Van Houtan, Monterey Bay Aquarium's director of science, and learn how plastic pollution keeps baby hawksbill turtles from thriving.
Tooth Leads to Re-think over Eotyrannus lengi Megan Jacobs, a palaeontology student from the University of Portsmouth, has something to remember from her trip to the Isle of Wight, for whilst exploring Compton Beach on the western side of the Island she discovered a 2.8 centimetre long dinosaur tooth that has been identified as belonging […]
Tuna crabs are back—tell a friend! Once again, swarms of pelagic red crabs—aka tuna crabs, Pleuroncodes planipes—beached themselves In Monterey yesterday. First seen in October of last year, there have been a handful of these mass strandings...Show More Summary
In this second article on maniraptorans, we look at the main groups that constitute this clade: you'll need to remember the main group names if the trends and tendencies discussed in later parts of this series are to make any sense.Show More Summary
Osteichthyans, or bony fishes, comprise two categories, each containing over 32,000 living species: Sarcopterygii (lobe-finned fishes and tetrapods) and Actinopterygii (ray-finned fishes). Nevertheless, actinopterygians have an obscure early evolutionary history. Show More Summary
The world's environment ministers are meeting in Nairobi this week to discuss major issues plaguing the planet, from wildlife trade to plastic in the ocean, and possibly agree on remedies
A rocket carrying the Reusable Launch Vehicle Technology Demonstrator (RLV-TD) lifted off from the Satish Dhawan Space Centre at 9:30 p.m. Eastern Sunday.The RLV-TD, a winged vehicle similar in appearance to the U.S. Air Force' s X-37B,...Show More Summary
The H3 Launch Vehicle is a liquid propellant launch vehicle currently under development. This is the first full-scale development of the 21st century. The aim of this development is to respond to launch demands from global customers....Show More Summary
When Mark Karpeles, the CEO of what was once the world's largest Bitcoin exchange, said that the company had gone bankrupt because 800,000 bitcoins (worth nearly half a billion dollars at the time) had been hacked, he wasn't exactly lying. Show More Summary
Characterization of aromaticity in analogues of titan's atmospheric aerosols with two-step laser desorption ionization mass spectrometryAuthors:Mahjoub et alAbstract:The role of polycyclic aromatic hydrocarbons (PAH) and Nitrogen containing...Show More Summary
luscious916: People watching and fish watching at the Monterey Bay Aquarium!???????????????? #Ocean #Monterey #Aquarium (at Monterey Bay Aquarium)


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|Fremont, Ohio
|Former Occupation
|Special education teacher
|6' 3''
|235 lbs.
|Top 10 Finishes (Wins)*
|3 (0) As of 4/18/2014
|Career Earnings*
|$52,824 As of 4/18/2014
|Current World Rank
|Last 3 Finishes
69 - Cayuga Lake, 8/21/2014
98 - Delaware River, 8/7/2014
83 - Lake Chickamauga, 6/11/2014
|Bassmaster Classics Fished (Won)
|0 (0) As of 4/18/2014
|FLW Championships Fished (Won)
|0 (0) As of 4/18/2014
|Angling Hero
|Kevin VanDam and Rick Clunn – "Both for setting the bar so high for the rest of us."
|Home Lake
|Erie (Western Basin)
|Favorite Lake
|Least Favorite Lake
|Ohio River – "I enjoy the fishing style, it's just tough fishing."
|Favorite Technique
|Hollow-belly frog
|Primary Fishing Strength
|Shallow-water cover
|Secondary Fishing Strength
|Great Lakes smallmouth
|Biggest Weakness
|Offshore fishing on Southern reservoirs
|Fishing Sponsors
|Jann's Netcraft, Lew's, Berkley, Spiderwire, Humminbird, Minn Kota
|Non-Fishing Sponsors
|Crown Battery
|Tow Vehicle (Sponsor)
|Ford Expedition
|Favorite Food
|Favorite Music
|Favorite Book/Movie
|Getting Stoned with Savages/The Legend of Bagger Vance
|Non-Angling Hero
|Parents – "They both are extremely good role models."
|When Not Fishing
|"I like to hike in the woods with my dog, catch crayfish, hang out with friends and fish in local streams and rivers."
|Why He Fishes
|"It allows me to stay outdoors and it's one of the most challenging things that's equally as frustrating as it is rewarding. I've been fascinated with fish ever since I was a little kid."
|Click a column heading to re-sort.
|Tournament Finishes
|(Only recent tournaments are included. Not necessarily a career listing.)
*BASS events (all events because BASS does not report Tour and Open earnings separately) and FLW Tour.
Biographical data last updated: 4/18/2014
You may report any errors or omissions by clicking here. Please be certain of your information before submitting anything. Submissions will be examined, and if approved will be included in a future update.
By Todd Ceisner BassFan Editor The 10th edition of the Toyota Texas Bass Classic was not the slugfest some have come to expect from the event that
By Todd Ceisner BassFan Editor As foreign a place as Lake Ray Roberts was to Matt Herren before he started practice for the Toyota Texas Bass Classic
By BassFan Staff Matt Herren left the dock this morning hoping he could figure out how to trigger some bites in the morning. Through the first 2 days


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Black-Clad Einsatzgruppen Confiscate Guns in Californiaby William Norman Grigg
Nov. 19, 2013
1.Trump Rips Bill Kristol: "All The Guy Wants to do is Kill People and Go to War"
2.Migrants Thank 89-Yr-Old Austrian Man Who Gave Them Euros by Robbing Him
3.Angry Birds Movie is Red-Pilled Anti-Immigration Propaganda
4.BUSTED: Katie Couric Anti-Gun Doc Deceptively Edited to Make Pro-Gunners Look Foolish
5.VIDEO: Anti-Trump Protester Spits on Asian-American Trump Supporter
6.VIDEO: BLM Lunatics Storm Stage, Threaten to Punch Milo at DePaul Event
7.Watch Anti-Trump Protesters Act Embarrassingly Ignorant
8.The Guardian's Steven Thrasher Plays Victim After His Anti-White Hate Video Goes Viral
Einsatzgruppen are special paramilitary task forces, the most notorious of which were the death squads deployed by Germany's National Socialist regime. The contemporary American soyuz is lousy with einsatzgruppen, some of which — such as the Pima County, Arizona SWAT team, which murdered Jose Guerena in his Tucson home – behave in a fashion almost indistinguishable from their German antecedents.
The State of California's Armed Prohibited Persons System (APPS) has created an einsatzgruppe dedicated exclusively to gun confiscation. Using gun registration lists, the APPS stormtroopers, "arriving in SUVs and dressed in black tactical uniforms … regularly sweep through California cities" to seize firearms from people the state has designated "prohibited persons," reports the Fresno Bee. A criminal conviction is not necessary for enrollment on the civilian disarmament register; all that is necessary is an official finding by the state's Welfare bureaucracy that the gun owner is "a danger to himself or others."
The searches are conducted without warrants — unless the targeted individuals refuse to cooperate, in which case the raiders will "seek a warrant and lock down the house until they get results." California Governor Jerry Brown recently signed a bill that will use a $24 million surplus from firearms purchase fees to hire 36 new jackboots for the squad.
So far, California is the only state to deploy a dedicated gun confiscation team — but it isn't the only one to engage in pre-emptive civilian disarmament. In 1999, the Connecticut legislature enacted a measure permitting police to confiscate firearms from any individual believed to pose "a risk of imminent personal injury to himself … or to other individuals." All that is necessary for the seizure to occur is a sworn complaint "by any state's attorney or assistant state's attorney or by any two police officers to any judge of the Superior Court." The confiscated firearms can be held for up to a year, without any criminal charges or civil action being taken against their owner. Thousands of firearms have been seized under that provision, which famously did not prevent the Sandy Hook Massacre.
In the People's Republic of Massachusetts, police and prosecutors didn't even bother pretending that they were enforcing a law permitting the pre-emptive disarmament of Gregory Girard, a resident of Manchester-by-the-Sea, after his estranged wife — who apparently is a temporally displaced subject of East Germany — called health and welfare officials to report that her husband held eccentric political views. Specifically, Mr. Girard believed that martial law, complete with gun confiscation, is imminent. Since it is impermissible for people to believe that government agents will carry out paramilitary raids to confiscate firearms, a paramilitary squad was sent to Girard's home to confiscate his firearms.
California's roving gun confiscation squad will be the template from which numerous anti-gun einsatzgruppen will be struck.


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...I use many creative ways to help each student thrive in their learning experience. I have been told by my students and parents that I am very patient, detailed-oriented and precise in creating a positive learning experience for them. I have worked as a U.S. college counselor, taught at the college, high school and adult school levels.
2 Subjects: including Chinese, ESL/ESOL
San Diego, CA
...I am a father, husband, Realtor, taco lover, a true surfer at heart, and love to speak and help others learn and speak Spanish. If you need a Spanish Tutor in North County San Diego, I'm your guy. I am not a native Spanish Speaker, so I am very easy to understand, and I can adapt my teaching style to your type of learning style.
1 Subject: Spanish
San Marcos, CA
...I studied French for 8 years and lived in France as well, where I did private tutoring and taught English to business people. I have more than 20 years of teaching experience. I work with students on a one to one basis in order to uncover where the gaps in understanding exist.
6 Subjects: including ESL/ESOL, French, English, writing
San Diego, CA
I have more than twenty years of experience teaching Farsi and for the last four year have been teaching Farsi to US Navy SEALs at a naval base in Coronado. I have acquired a certificate of appreciation from naval advanced training command, and in the last four years all of my students have passed ...
1 Subject: Farsi
Carlsbad, CA
...The University of Turabo has equipped me with the necessary education and credentials to more than adequately teach and tutor here on the island. I impart dignity and respect upon my students propelling them to seek higher education and to reach their maximum potential while acquiring personal g...
8 Subjects: including ESL/ESOL, TOEFL, Microsoft Excel, vocabulary
San Marcos, CA


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Bargnani out with sore lower back
Toronto - Toronto Raptors center Andrea Bargnani will not play against the Bucks on Friday night due to a sore lower back.
Bargnani was scratched from the Toronto lineup about an hour before game time. The 7-foot Bargnani played 31 minutes in Milwaukee on Wednesday night and scored 10 points on 4-of-13 shooting.
Rasho Nesterovic made the start in place of Bargnani. It was only the second start of the season for the 7-foot Nestervoic, who also started in Milwaukee on Dec. 9 when Bargnani missed the game due to a sore right ankle.
Bucks coach Scott Skiles said his team is facing a major challenge to beat the Raptors in back-to-back games. Chris Bosh scored a career-high 44 points at the Bradley Center two nights ago.
"We'll have to play one of our better games all-around to win," Skiles said. "We've got to do a much better job on Bosh, and a better job on (Jose) Calderon. Then we can't let the other guys come into the game.
"We did a decent job on (Hedo) Turkoglu. We need to do all those things again and up our effort. We were shooting so poorly in the first quarter. We certainly don't want to come out in their building and do that.
"We were able to come out in the second half and re-take control of the game. We don't want to be playing uphill against these guys tonight."
- Bucks Podcast: Gardner & Velazquez discuss the draft, free agency and other off-season happenings
- Tar Heels' Marcus Paige in Bucks workout
- Maryland guard Melo Trimble tests skills for Bucks
- Bolomboy, English lead Bucks first workout
- Giannis finishes third in NBA most improved player voting
- Bucks Podcast: Wrapping up the 2015-'16 season
- Bucks lose draft tiebreakers; hold 10th spot
- Upbeat Plumlee says he wants to return to Bucks
- Middleton honored with PBWA award
- Retiring ref Joey Crawford did it his way


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The owner a relatively young man was very helpful to me in my genealogical research I tried to conduct at the old cemetery. He looked through the data the best he could and assisted as much as possible. It is my hope in the future to access his records and place in interment.com and findagrave.com the relative data from the cemetery. This in turn will assist many family genealogists and his business with upgraded grave stones and alike at the cemetery. The owner seemed to understand the desire of genealogist to fill their voids in research.
Joseph Lee Cemeteries
61. Maryland Veterans Cemetery Garrison Forest
11501 Garrison Forest RdOwings Mills, MD 21117
62. St John's Cemetery
3480 Saint Johns LnEllicott City, MD 21042
63. Arlington Cemetery Of Chizuk
8100 Stevenson RdPikesville, MD 21208
64. St John's Cemetery
ST Johns LnEllicott City, MD 21043
65. Good Shepherd Cemetery
2805 Rogers AveEllicott City, MD 21043
66. Har Zion Tifereth Cemetery
7504 7 Mile LnPikesville, MD 21208
67. St John Cemetery
13305 Long Green PikeHydes, MD 21082
68. Saints Rest Cemetery
7310 Ridge RdHanover, MD 21076
69. Kaczorowski Funeral Home
We were in the process of making pre-arrangements for my mother and had visited 4 places. When we were there we talked to Bobby. The decision was up…
70. St Stanislaus Cemetery
6515 Boston StBaltimore, MD 21224
71. Lilly & Zeiler Inc
700 S Conkling StBaltimore, MD 21224
From Business: Since our founding in 1908 by Edward D. Lilly and my father, Charles G. Zeiler, Lilly & Zeiler Inc. funeral home, has remained a family owned and operated business.…
72. David J Weber Funeral HomesServices
401 S Chester StBaltimore, MD 21231
73. Mitchell-Wiedefeld Funeral Home Inc
6500 York Rd.,Baltimore, MD 21212
From Business: Since 1837, Mitchell-Wiedefeld Funeral Home Inc. has been serving families in Baltimore and the surrounding areas. The facility offers traditional funeral, direct c…
74. Loudon Park Cemetery
3620 Wilkens AveBaltimore, MD 21229
75. Simplicity Plan
3620 Wilkens AveBaltimore, MD 21229
76. United States Government
5501 Frederick AveCatonsville, MD 21228
77. Greene Funeral Service
5151 Baltimore National PikeBaltimore, MD 21229
78. W S Tegeler Monument Co
5804 Windsor Mill RdGwynn Oak, MD 21207
79. Harry H Witzke Family Funeral Home
4112 Old Columbia PikeEllicott City, MD 21043
From Business: The original Harry H. Witzke Funeral Home was founded in 1912 in Baltimore. One location was at Hollins and Gilmore Streets, and another was at 4101 Edmondson Avenu…
80. Merkle Monuments
8112 Liberty RdWindsor Mill, MD 21244
81. Raymond G Merkle Inc
8112 Liberty RdWindsor Mill, MD 21244
82. McCully-Polyniak Funeral Home
3204 Mountain RdPasadena, MD 21122
83. Sterling Ashton Schwab Witzke Funeral Home
1630 Edmondson AveCatonsville, MD 21228
84. Chesapeake Monuments
11417 Reisterstown RdOwings Mills, MD 21117
From Business: At Chesapeake Monuments we pride ourselves on making the finest quality memorials at affordable prices. No two lives are the same, and we believe that the same shou…
85. Eckhardt Funeral Chapel
11605 Reisterstown RdOwings Mills, MD 21117
From Business: Our Owing Mills funeral home was established in 1961 by Jim and Patricia Eckhardt in a Pre-Civil War log cabin in the area formally known as Delight. The facility h…


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5 injured in escalator accident at NJ rail station
Associated Press
JERSEY CITY, N.J. (AP) – Screams and panic engulfed a commuter rail station Monday morning when an ascending escalator changed direction and started to head back down, causing some people to jump off mid-ride.
A spokesman for the Port Authority of New York and New Jersey, which operates the station, said five people were injured when the escalator malfunctioned around 9 a.m. at the Exchange Place PATH station in Jersey City.
Three were taken to the hospital as a precaution, according to Port Authority Spokesman Ron Marsico. None of the injuries was considered life-threatening. They ranged from back and neck pain to scrapes, bumps and bruises, Marsico said.
Nick Lukish, of Brooklyn, N.Y., said he was headed to work and was midway up the escalator when the moving staircase started going down.
"There was a stampede at the base of the escalator," Lukish said. "People started to panic and yell and scream, and I saw some people jump over to the down side of the escalator, so I jumped."
The 33-year-old, who works in information technology at a bank in Jersey City, said it wasn't until he got to work that he noticed he had cuts and bruises on his shins from the jump. After going to a pharmacy to get some bandages, he returned to the station to give his account to police.
A YouTube video showed some people jumping onto the divider between the up and down escalators as others shouted in alarm or tried to keep walking up.
Two of the station's three escalators were shut down after the incident, forcing thousands of commuters to walk up the 128 stairs to the street.
The Exchange Place station suffered extensive damage during Superstorm Sandy. PATH officials were investigating what caused Monday's escalator malfunction, "including whether Superstorm Sandy-related effects played a role," Marsico said.
Escalator service at Exchange Place will be suspended through Tuesday morning, Marsico said, adding that workers were looking at all PATH escalators throughout the system.
(Copyright 2013 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.)
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