text:
|Publication number
|Publication type
|Application number
|Publication date
|Feb 13, 2003
|Filing date
|Jun 27, 2002
|Priority date
|Oct 2, 1991
|Also published as
|Publication number
|Alan Smith
|Original Assignee
|Genzyme Corporation, A Delaware Corporation
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (5), Classifications (19), Legal Events (9)
|External Links: USPTO, USPTO Assignment, Espacenet
[0001] The present inventions involves the large-scale production of membrane specific proteins and more particularly involves the production of such proteins using transgenic animals and most specifically involves the production of CFTR, the protein involved with Cystic Fibrosis.
[0002] Striking progress has been made in our understanding of cystic fibrosis (CF) during the period since the gene associated with the disease was identified1-3. Many mutations within the gene have been identified in DNA from patients with CF4-10. The protein product of the gene, named CFTR, has been identified11 and functional studies have shown that CFTR cDNA is able to complement the defect in ion transport characteristic of cells from CF patients12,13. The domain structure of CFTR has been probed by analysis of mutated versions of the protein14-18. Such studies indicate that CFTR is a Cl− channel14,19,20 and that it is required by phosphorylation of the R-domain16,18,21 and by nucleotide binding22. to our knowledge none of such proteins have involved exogenous or recombinant membrane proteins.
[0003] In accordance with the principles of the present invention it has been surprisingly discovered that exogenous or recombinant membrane proteins can be produced in the milk of transgenic animals. In the most preferred embodiment of the present invention, recombinant CFTR has been produced in the milk of transgenic mice containing CFTR cDNA downstream of a mammary specific promoter. Most preferred embodiments will employ larger transgenic animals including, for example, rabbits, goats, sheep and cows which produce large quantities of milk however, the development of such animals takes considerably longer than the time periods required for developing transgenic mice. While the time periods vary considerably, the procedures and methods are substantially identical.
[0004] Other embodiments include the apocrine like secretion in tissue culture of membrane proteins by cells which have been maintained in a differentiated state. Such proteins would be secreted as part of the membrane released during the "pinching off" proces. Membrane proteins suitable for production according to the methods of the present include receptors, channels, viral glycoproteins, transporters and other proteins typically associated with cellular membranes. Such membrane proteins can be used for therapeutics such as by administration of the abnormally deficient or missing protein, or by use as vaccines in the case of viral glycoproteins including for example utilization of the envelope of HIV, Herpes Virus or influenza in order to develop immunity with respect to infection caused thereby.
[0005] Advantageously, methods of the present invention can also be employed for the production of membrane proteins useful for diagnostic purposes. For example, one could use the present methods to produce the receptor for thyroid stimulating hormone (TSHR) which would be useful for the diagnosis of Graves Disease. Such proteins could also be used to screen for therapeutically active compounds.
[0006] Preferred methods of the present invention will employ promoters, ideally coupled with suitable enhancers, that are mammary specific so that production of the desired membrane protein is incorporated into milk fat globules which will appear in the milk of a lactating female transgenic animal. Such an animal is, of course, by definition, reproductively competent. Greater appreciation of these and other emodiments will be acquired by study of the following drawings, examples and detailed procedures.
[0007] Further understanding of the present invention may be had by reference to the figures wherein:
[0008]FIG. 1(a) shows the structure of the mammary specific expression vector for producing CFTR using abbreviations: S, Sal I; R, EcoR I; N, Not I;
[0009]FIG. 1(b) shows identification of positive transgenic mice by Southern blot analysis;
[0010]FIG. 2(a) shows the detection of CFTR in milk of transgenic mice;
[0011]FIG. 2(b) shows one-dimensional peptide analysis of CFTR synthesized in recombinant C127 cells and in transgenic mice milk;
[0012]FIG. 3 shows the digestion of milk-derived CFTR with glycosidases; and
[0013]FIG. 4 shows fractionation of milk from transgenic mice; and FIGS. 5A, B and C graphically show relevant vector constructions.
[0014] The 17.8 Kb DNA consruct used to produce transgenic mice capable of secreting CFTR in their milk is shown in FIG. 1A. It consists of a full length copy of CFTR cDNA (containing a point mutation at residue 936 to inactivate an internal cryptic bacterial promoter that otherwise renders the cDNA unstable15 ) inserted between exons 2 and 7 of the goat β-casein gene. The β-casein gene contains a mammary gland specific promoter which results in expression of the desired gene, e.g. CFTR or other membrane protein, within the mammary gland. Other milk specific promoters which may be used in substitution include well known promoters such as ∝-casein, whey acid protein and β-lactoglobulin promoters. Most preferred constructs will include one or more enhancer elements typically associated with such genes as has been described previously in the art. Such promoters/enhancers are then associated with the coding sequence of the membrane protein of interest using conventional recombinant techniques. Such coding sequence may be either cDNA, partial or fully genomic DNA but more preferably either of the latter two categories since such have been shown in transgenic systems to cause greater levels of expression. More particularly, the 4.5 kb Sal I-Sal I fragment from pMT-CFTR15 was cloned into the Xho I site of CAS 1441 (see also FIG. 5C). The cosmid vector CAS 1441 contains an altered goat β-casein gene with an Xho I site in place of the coding portion of the gene. Earlier studies have characterized the goat casein gene and shown that it directs the synthesis of a number of gene products in the milk of both transgenic mice and goats in a tissue-specific manner26,27. The portion deleted extends from the Taq I site in exon 2 to the PpuM I site in exon 7.
[0015] With further reference to Figure series 5, a goat genomic DNA library was constructed by cloning DNA fragments generated by partial MboI digestion of Saanen goat DNA into EMBL3 phage as described by Maniatis et al. 31. The library, consisting of 1.2�106 recombinant phage, was screened with a 1.5 Kb HindIII/TthIII1 DNA fragment encoding the entire mouse beta casein cDNA as described by Maniatis et al. 31. Three plaques designated 3-7, 3-8, and 3-11 exhibited the strongest hybridization signals under conditions of high stringency and each were subjected to three rounds of plaque purification. Restriction enzyme and Southern blotting analysis revealed that the inserts within recombinant phage 3-7 and 3-8 were subfragments of the 18.5 Kb insert contained in phage 3-11. Therefore, all DNA fragments used for construction of a mammary gland specific expression vector were derived from clone 3-11 which is shown in FIG. 5A. All subclones were constructed in pUC or pUC derivatives with modified polylinker.
[0016] To construct a mammary gland specific expression vector, the entire upstream (5′ portion) region from the SalI site to exon 2 and the entire downstream (3′ portion) region from exon 7 to the SalI site of the goat beta casein gene was used to direct expression to the mammary gland. To engineer the 5′ end of the beta casein gene, the TaqI site in exon 2 of clone Bc106 was replaced with a BamHI restriction site to produce the plasmid Bc150 (FIG. 5A). The entire available 5′ region of the goat beta casein gene was constructed by the sequential addition of the subclones Bc104, Bc147, and Bc103 (FIG. 5A). The orientation of subclone Bc147 was verified by restriction analysis. The final vector designated Bc113 contains a SalI site at the 5′ end and a BamHI site at the 3′ end. The downstream BamHI was subsequently changed to an XhoI site to form Bc114.
[0017] The 3′ end of the goat beta-casein gene was constructed in a similar fashion to the 5′ end. The 1.8 Kb Bc108 clone (FIG. 5A) was digested with PpuMI to allow for the addition of a BamHI linker. The BamHI/HindIII fragment from the engineered clone Bc108 was ligated into the vector Bc109 which contained the extreme 3′ flanking region of the goat beta casein gene. The entire 3′ end was completed by the addition of the 4.4 Kb HindIII fragment from Bc108 was cloned into the modified Bc109 vector and screened for orientation. The new vector was designated Bc118 and contained the entire 3′ region of beta casein from exon 7 through the poly A signal and 5 Kb downstream to the SalI site of EMBL clone 3-11. Subsequently, an XhoI site was introduced at the 5′BamHI site and a NotI site at the 3′ SalI site of Bc118 to produce the vector Bc122.
[0018] The overall cloning strategy followed to construct the beta casein CFTR vector (Bc8) is shown in FIG. 5B and 5C. To construct a goat beta casein vector containing all the available upstream and downstream sequences, a 1.7 Kb XhoI to SalI stuffer fragment containing a unique SacII site cloned into the XhoI site of Bc114 and Bc122 to form vectors Bc143 and Bc144, respectively. The complete vector was made by cloning the SalI to SacII fragment from Bc143 into a SalI to SacII cut Bc144 to form the plasmid Bc6. The stuffer fragment was removed from the plasmid by digestion with BamHI and a unique XhoI site was inserted to form the vector Bc145. The SalI to NotI fragment from Bc145 which contains the 5′ and 3′ beta casein sequences was subcloned into the cosmid clone CAS1438 to form the cosmid CAS1441. CAS1438 is a cosmid vector constructed in pHC79 containing an engineered SalI and NotI site and 21 Kb of 5′ flanking sequence from the Bovine alpha-casein gene32 (Meade et al. Biotechnology 8, 443-45 1990). The expression vector Bc8 was constructed by digesting the vector CAS1441 with XhoI and ligating in the 4.5 Kb SalI fragment from pMT-CFTR11,17. Orientation of the CFTR cDNA was confirmed by restriction analysis. To obtain a fragment for microinjection, the vector Bc8 was digested with SalI and NotI to release the beta casein CFTR portion of the vector for purification. The final microinjection fragment contained 4.2 Kb of 5′ flanking sequence, exon 1, intron 1, a portion of exon 2, 4.5 Kb CFTR cDNA, a portion of exon 7, intron 7, exon 8, intron 8, exon 9, and 5.3 Kb of 3′ flanking sequence. This fragment from Bc8 was injected into mouse embryos and reimplanted using standard methods. Transgenic mice were identified by Southern blot analysis of DNA from the tails of resulting offspring.
[0019] To screen for positive transgenic mice, approximately 15 μg of genomic DNA from each founder mouse was digested with EcoR I, separated on a 1% agarose gel, transferred to magnagraph nylon (Fisher Scientific and hybridized with the 384 bp Pvu II-Pvu II fragment from exon 7 of the mouse β-casein cDNA30 and the 4.5 kB SaI I-SAI fragment of pMT-CFTR. Copy number was quantitated using a Betascope 603 Analyzer.
[0020]FIG. 1B shows DNA from the positive founder animals. Band I is mouse β-casein DNA and bands II, III and IV are CFTR DNA. Lane 1 is a control mouse and lanes 2 to 5 show results of four founder transgenic mice. Such analysis indicates the copy number of the transgene in the lines varied between 1 and 10.
[0021] Founder mice were bred to produce lactating transgenic females. Milk from such animals was collected, diluted with PBS (phosphate buffered saline) and treated with RIPA (50 mM Tris-HCI [pH 7.5], 150 mM NaCI, 1% Triton X-100, 1% sodium deoxycholate and 0.1% sodium dodecyl suplhate) buffer to solubilize membrane proteins. Briefly, samples were examined for the presence of CFTR by immunoprecipitation with pAbEx13, a highly purified polyclonal antibody raised against an R-domain fusion protein (see Canadian Patent Application 2,037,478-1, fully incorporated herein by reference), followed by in vitro phosporylation treatment of the washed immunoprecipitate with protein kinase A(PKA) in the presence of [y 32P]ATP11,15. More specifically, procedures for preparing cell lysates immunoprecipitation of proteins using pAbEx13, one-dimensional peptide analysis and SDS-polyacrylamide gel electrophoresis were as described by Cheng et al. 15,16. In vitro phosphorylation of the CFTR-containing immunoprecipitates was achieved by incubating with protein kinase A and [y 32P]ATP (10 μCi) in a final volume of 50 μl in kinase buffer (50 mM Tris-HCI, pH 7.5, 10 mM MgCl2 and 100 μg ml−1 bovine serum albumin) at 30� C. for 60 min. Milk was collected from the transgenic mice at days 3, 5, 7 and 9 of lactation. Mice were injected with oxytocin (2.5 IU) and milked 5 min later with a suction device. The milk was diluted (10 fold) and solubilized in RIPA buffer prior to immunoprecipitation.
[0022]FIG. 2A shows a polyacrylamide gel of samples from milk of two females of line #12 and a control mouse. Lane 1 contains an iminunoprecipitate from approximately 3�106 recombinant C127 cells stably transfected with a wild type CFTR cDNA. Immunoprecipitates of 50 μl of control milk (lane 2), milk from transgenic mouse #12-6 (lane 3) and mouse #12-9 (lane 4) were subjected to the same treatment. The positions of the fully processed CFTR (band C) and the partially glycosylated form (band B) are indicated on the right margin. Exposure time was 30 min at −70� C. As can be seen from the results, a diffuse band of labeled protein is detected in the milk from both of the transgenic mice but not the control. The protein (lanes 3 and 4) migrates slightly more rapidly than the fully processed version of CFTR (lane 1) from stably transfected mouse C127 cells containing a BPV-CFTR vector. Such differences in migration of CFTR have been detected previously in samples of the protein from various recombinant and high producer cells. They have been shown to be caused by differences in glycosylation15,20.
[0023] To confirm that the protein detected in FIG. 2(a) is indeed CFTR, the labeled protein was eluted and subjected to partial proteolytic cleavage using Staphylococcus aureus V8 protease followed by polyacrylamide gel analysis. Specifically, the vitro labeled bands C from recombinant C127 cells (lanes 1-3) and from mouse #12-6 (lanes 4-6) were excised from the gel and digested with different amounts of Staphylococcus aureus V8 protease11,15. Proteins in lanes 2 and 5 were digested with 0.17 μg μg−1 S. aureus V8 protease and those in lanes 3 and 4 with 0.017 μg μl−1 enzyme. Lanes 1 and 6 were untreated samples. Exposure time was 24 h at −70� C. The results depicted in FIG. 2(b) show that the milk derived protein yields a fingerprint indistinguishable from that of CFTR derived from C127 cells. Thus, the milk from line #12 mice contained authentic CFTR. Similar analysis of milk from the other transgenic mouse lines for the presence of CFTR showed that line #38 contained CFTR but in lesser amounts than in line #12 whereas lines #18 and #13 contained no detectable protein.
[0024] To confirm that the difference in mobility between milk-derived and C127 cell-derived CFTR results from different patterns of glycosylation, the 32P-labeled proteins were subjected to digestion with N-Glycanase� enzyme, endoglycosidase H and endoglycosidase F. In particular, the CFTR immunoprecipitated from the milk of transgenic mouse #12-9 and from recombinant C127 cells were phosphorylated in vitro using protein kinase A and [y 32P]ATP. Following elution from polyacrylamide gels, the CFTR proteins were either subjected to no treatment (lanes 1,3, 5 and 7) or were incubated with N-Glycanase (lanes 2 and 4), endoglycosidase H (lane 6) or endoglycosidase F (lane 8). Samples were separated by electrophoresis and analyzed by autoradiography. The positions of the fully processed form of CFTR (band C) and the non-glycosylated version (band A) are indicated on the right margin. Exposure was for 24 h at −70� C. The enzymes N-Glycanase, endoglycosidase H and endoglycosidase F were from Genzyme Corp. Conditions for digestion with the respective glycosidases were as specified by the manufacturer, except that incubations were performed at 37� C. for 4 h only. CFTR was immunopurified using the polyclonal antibody pAb Ex13, phosphorylated in vitro with protein kinase A and [y 32P]ATP, separated on SDS-polyacrylamide gels, and then extracted from the gels by macerating the gel pieces in protein extraction buffer (50 mm ammonium bicarbonate, 0.1% sds and 0.2% β-mercaptoethanol). Eluted proteins were recovered by trichloroacetic acid precipitation.
[0025] The results depicted in FIG. 3 show that milk CFTR is digested by N-glycanase to yield a band of greater mobility (lane 2) that co-electrophoreses with the corresponding band from C127 cell-derived, N-glycanase-treated CFTR (lanes 4). This band consisted of non-glycosylated CFTR11,15. As shown previously for COS-7 derived CFTR15, milk derived protein is resistant to cleavage by endoglycosidase H or F (lanes 6 and 8). It is therefore clear that the protein detected in the milk of transgenic mice is a fully glycosylated form of CFTR that contains carbohydrate side chains of somewhat less complexity than CFTR from recombinant C127 cells. In separate studies tissue plasminogen activator (tPA) produced in recombinant cells has been compared with that produced in milk of transgenic goats and likewise shown to have differences in the pattern of glycosylation26.
[0026] We surprisingly discovered that membrane proteins such as CFTR can be expressed in milk. We discovered that CFTR is present in the membrane of milk fat globules and thus is found in the lipid-rich fraction of the milk, that is, in the cream. To isolate the CFTR, milk from transgenic animals was fractionated as described by Imam et al. and Patton & Huston29. In summary, milk was first centrifuged at 3000 g to separate the cream which floats from the skim milk. The bulk of the caseins were separated from the skim milk by further low speed centrifugation at 12,000 g. Since milk fat globules are fragile, some membrane fragments remain in the supernatant of centrifuged skim milk23,29. Such residual membrane vesicles and fragments were collected by further high speed centrifugation at 100,000 g. In detail, 0.4 volume of wash buffer (10 mM Tris-HCI, pH 7.5, 0.25 M sucrose and 1 MM MgCI2) was added to whole milk (approximately 300 μl) and centrifuged at 3000 g for 15 min at room temperature to separate the cream. The resulting skim milk was siphoned off by inserting a needle through the layer of cream. The cream was washed twice with approximately 5 volumes of wash buffer and dispersed in RIPA buffer. The siphoned skim milk was centrifuged at 12,000 g for 10 min to pellet the caseins. The casein pellet was washed once with 5 volumes of wash buffer and resuspended in RIPA buffer. Membranes and vesicles contained in the resultant skim supernatant were collected by further centrifugation at 100,000 g for 2 h.
[0027]FIG. 4 shows PKA immunoprecipitation assays of the various fractions obtained. Whole milk (lane 1), the cream fraction (lane 2), the casein-containing pellet (lane 3), the 100,000 g skim pellet (lane 4) and the 100,000 g skim supernatant (lane 5) were solubilized with RIPA buffer, immunoprecipitated with pAb EX13 and phosphorylated in vitro using protein kinase A and (y 32P)ATP. Exposure time was 1 h at −70� C. About 65% of the CFTR was present in the cream (lane 2) and the remaining CFTR was in the high speed pellet from the skim milk fraction (lane 4). Virtually no CFTR was present in the casein fraction nor in the high speed supernatant.
[0028] The data shown hear clearly indicates that authentic CFTR can be produced in the milk of transgenic mice. The form of protein produced represents mature fully glycosylated CFTR rather than a partially glycosylated intracellular intermediate as indicated by its insensitivity to endoglycosidase H but sensitivity to N-glycanase. This pattern of sensitivity implies CFTR has a carbohydrate structure consistent with it having traversed both the endoplasmic reticulum and the Golgi, but why is it present in the milk ?
[0029] This surprising and unexpected result must occur because some, perhaps all, mature CFTR must reach the apical plasma membrane of mammary epithelia in lactating animals. This epithelia is actively involved in high level secretion, not only of soluble milk proteins such as the caseins through the normal protein secretion pathway, but also of lipids in the form of fat globules23,24. It has been discovered therefore that CFTR is secreted by apocrine like secretion into the milk rather than remaining in the cellular membrane. If the CFTR is not secreted as a soluble protein, but as part of the milk fat globule. Such globules are composed of an outer covering of pinched-off plasma membrane, a small amount of underlying cytoplasm comprising the so-called cytoplasmic crescent and a globule of fat23,24. Whether CFTR is incorporated into the fat globule membrane by a random process of plasma membrane blebbing or whether it is actively recruited to the exiting vesicle as it pinches off remains unknown. The demonstration that most CFTR in milk floats away from the bulk of milk proteins upon centrifugation, and the remainder separates from casein and can be sedimented at high speed strongly suggests that CFTR is present in membrane structures some of which include fat globules. The most plausible explanation of the results is that the bulk of CFTR exits the cell in the membrane of milk fat globules and that some of these globules break off membrane vesicle fragments upon handling. Alternatively, perhaps some empty membrane vesicles containing CFTR but no fat globules are directly shed from the mammary epithelia.
[0030] As a result of the present discovery that CFTR can be sequestered into milk, it will be readily apparent to those skilled that other human membrane-associated proteins such as receptors (e.g. cytokine receptors, hormone receptors), viral glycoproteins, transporters and channels that sort at least in part to the apical surface may be produced by the transgenic methods of the present invention. Alternatively, such membrane related proteins can be produced in vitro such as by tissue culture of appropriate cells capable of apocrine secretion. Mammary cells maintained in a differentiated state would be the preferred embodiment of such an approach. Although any cell capable of blistering or "blebbing-off" membrane associated proteins such as those associated with apocrine secretion will suffice. Previous demonstration that the synthesis of soluble human proteins in transgenic milk can be scaled up to production in transgenic goats26,27 confirms that virtually unlimited amounts of membrane proteins could be produced by this means.
[0031] The application of the present invention to CFTR production will enable the development of new therapies for CF. Of direct implication is the capability to now produce sufficient CFTR protein to allow therapeutic treatment of CF patients by protein replacement. In such therapy, CFTR protein is administered to the airway cells, preferably by aerosolization and inhalation, typically in conjunction with helper agents to assist in delivery the CFTR to the airway cell surfaces through the mucous layer for incorporation into the airway cell membrane. Because there is turnover of such cells, treatment by this modality is a continuous prospect.
[0032] Other exciting opportunities now enabled by the present invention include protein replacement therapies for other defective or deficient membrane protein mediated diseases as well as diagnostic and screening assays previously made impossible due to the absence of commercially viable membrane protein production systems since membrane proteins are not secreted into the surrounding medium. For example, the ability to now produce identified and sequenced cytokine receptors allows one to create not only an assay with a substrate (using either purified receptor or the milk fat globule itself or membranes therefrom having the receptor exposed) specific for the cytokine (whose binding to the substrate could be readily detected by a labeled, cytokine specific antibody) but can also be used as a screening device to detect other proteins or peptides capable of binding to the same receptors. Such peptides might serve to activate the receptor or alternately as antagonists to prevent cytokine function. Selection of new effective drugs would be facilitated using such a screening approach. Similarly, these techniques would be equally useful for hormone receptors such as those specific for thyroid stimulating hormone as earlier mentioned.
[0033] 1. Kerem, B. -S. et al., Science 245, 1073-1080 (1989).
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[0037] 5. Dean, M. et al.,, Cell 61, 863-870 (1990).
[0038] 6. Kerem, B. -S. et al., Proc. Natl. Acad. Sci. USA 87, 8447-8451 (1990).
[0039] 7. Kobayashi, K., Knowles, M. R., Boucher, R. C., O'Brien, W. E. & Beaudet, A. L. (1990). Am. J. Hum Genet. 47, 611-615 (1990).
[0040] 8. White, M. B. et al., Nature 322, 467-470 (1990).
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[0042] 10. Zielenski, J. et al, Genomics 10, 229-235 (1991).
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[0049] 17. Gregory, R. J. et al., Mol. Cell. Biol. 11, 3886-38983 (1991).
[0050] 18. Rich, D. P. et al., Science 235, 205-207 (1991).
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Burnt bodies of woman, minors found in West Bengal
Partially charred bodies of a woman and two minor girls, including an infant, were found Sunday from inside a building in West Bengal's Burdwan district, police said.
The bodies were discovered from inside a school building in the Kanksa police station aras in the district, some 150 km from Kolkata.
"We found the dead body of a female aged around 28 years with a five-month baby girl in her arms and a girl of seven years. The woman's hands were tied with a rope and her face burnt," Superintendent of Police S.M.H. Meerza said.
The bodies which are yet to be identified, have been sent for post-mortem with the police suspecting they were murdered before their bodies were set on fire.
"The victims were perhaps killed somewhere else and their bodies dumped here," added Meerza.
(Posted on 25-08-2013)

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This 3 DVD Set Presents the Magic,
the Inventions and the Story of U. F. Grant.
Included on the DVDs:
Rare lost film lectures
Original drawings of Grant?s illusions
Vintage film footage with Gen Grant Jay & Francis Marshall and other greats
Interviews with people who knew Grant
Color film footage of Grant demonstrating his creations
Experience the history of this most amazing magician, inventor and teacher.
There are also a host of special features you will want to see including downloadable copies of Grant's most popular manuscripts & a slide show tour showing how the effects came to be.
This is a once in a lifetime opportunity to see
and hear 'inside the inventive process'.


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Lol - James Whitham is from Huddersfield in Yorkshire.
I was at this race weekend - Brands Hatch British Superbikes FYI.
Didn't see this live though, only on the big screen as i was on the other side of the track.
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The Pacific - Asian Studies Program offers three tracks in Chamorro Studies, East Asian Studies and Japanese Studies. Each track is built on traditional UOG strengths, and covering overlapping linguistic, economic, historical, cultural, and philosophical areas, which can be investigated in varying emphases depending upon the individual student's interests and goals. Profiting from Guam's close geographical proximity and strong socio-economic interconnections to the objects of its study, this cultural studies program enables robust studies of the languages, civilizations, and societies of Japan, China, Korea, and neighboring areas. While aiming to recognize and clarify the unique characteristics of each Pacific-Asian society, the program rests on the traditional conviction of liberal, humanistic education, that each of these regions constitutes part of the common human heritage.
The Chamorro Studies track develops students' affective capacity for an appreciation of cultural diversity, human and indigenous rights, and social and communal responsibility. Creative capabilities in the areas of oral and written literary expression will also be developed. Chamorro Studies covers an interdisciplinary range of subjects, examining knowledge pertaining to Chamorro identity and subjectivity. It draws on courses in Chamorro Language, Anthropology, Biology, Literature, Geography, History, Political Science, and Psychology. Chamorro Studies is not offered elsewhere, although BA programs in Pacific Islands Studies are offered at the University of Hawaii at Manoa, Hawaii Pacific University, Brigham Young University-Hawaii, and Australian National University.
Required Courses (25 credit hours): CM201, CM202, CM301, CM302, HI211, CM340, CM412.
Electives (12 credit hours, at least 9 hours upper division): AN320, AN325, AN/HI413, AN421, BI100, BI103, BI201, EN333, EN461, GE401, HI243, HI444, HI450, PS225, PS412, PY475, or upper division CM courses.
Required Courses (22 credit hours): CM101, CM102, CM201, CM202, HI211, CM340.
Electives (6 credit hours): AN320, AN325, AN/HI413, AN421, BI100, BI103, BI201, EN333, EN461, GE401, HI243, HI444, HI450, PS225, PS412, P 475, or upper division CM courses.


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Laura Marling unveiled as Green Man Festival 'special guest'
Singer-songwriter will play Main Stage on the Sunday
Photographer: Al De PerezDaniel Fahey on 04 May 2011
The singer-songwriter, known for albums 'I Speak Because I Can' and 'Alas, I Cannot Swim', will
play the Main Stage on the Sunday (21 August).
She joins The Avett Brothers, Destroyer, Josh T Pearson and Alessi's Ark, who were announced previously.
Fleet Foxes, Explosions In The Sky and Iron and Wine will headline the Welsh festival with more confirmed as part of the Green Man Festival line-up.
Click here to see the latest Green Man Festival line-up.
Tickets are on sale now at £135 for an adult pass, with concessions available for teenagers and students.
Click here to buy Green Man tickets.


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Corrections & Amplifications
Pimco Investment Grade Corporate Bond Fund has outperformed its benchmark, the Barclays U.S. Credit Index, which is down 4.36% in 2013, as of Monday. A Money & Investing article on Wednesday about Pimco bond manager Bill Gross, and accompanying chart, incorrectly said the fund used the Barclays U.S. Aggregate Bond Index as its benchmark. A corrected chart is on page C10.
SRI International is a Menlo Park, Calif., nonprofit research institute. A Personal Journal article on Tuesday about the quest for effective sleep drugs incorrectly said it is based in Palo Alto, Calif.
The Seattle Marinershave eight players who spent time in the minor leagues. In some editions Wednesday, a chart with a Sports article listed the Mariner players but incorrectly said they played for the Washington Nationals.
Dune Real Estate Partners LP, which was spun off from Dune Capital Partners, is an investor in a new condominium at 56 Leonard St. A Property article Wednesday misidentified Dune Capital as the investor.
Samuel Jamier described "The Animals" as the "Filipino 'Spring' Breakers." An earlier version of a Greater New York article published Thursday incorrectly quoted Mr. Jamier as describing the 2012 drama as the "Korean 'Spring Breakers.'"


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'Dream come true' for Falcao after joining Man U on loan from Monaco
Colombian striker Radamel Falcao has declared that playing for one of the truly great teams like Manchester United had been a lifetime ambition, after the Premier League club fetched out around 20 million pounds to bring him from AS Monaco for just one loan season.
Falcao was desperate to leave French club Monaco and, though United were not his first choice, their offer was the only one on the table.
But the striker said of his new club that it is a dream come true to join a club like United, one of the biggest clubs in the world. He added that he has always dreamt about playing for one of the truly great teams and winning lots of trophies, The Independent reported.
Falcao said that it is a once in a lifetime opportunity and one cannot turn that down. He added that he is a huge fan of English football and watch it whenever he can and now the reality is that he is going to play there.
The 6 million pounds signing-on fee United have agreed to pay for the Colombian takes their outlay on players beyond 150 million this summer. But it is by agreeing to Falcao's wages that they take the biggest financial hit.
Other Premier League clubs were quoted 18 million pounds a year, 3,46,000 pounds-a-week, when Falcao's representatives first began touting him around, midway through last week.
However, United are understood to have agreed to pay 2,65,000 pounds-a-week, making Falcao an even bigger earner than Wayne Rooney and Robin van Persie. United have an option to buy the player for 43.4 million pounds at the end of the season, the report added.
(Posted on 02-09-2014)
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text:
Protests Continue Against Egyptian President Mohammed Morsi
A protester leans against a concrete wall suffering from exposure to... Fotografia de notíciasBetão,Cairo,Conflito,Crise,Dor,Egipto,Exposição Longa,Heliópolis,Horizontal,Inclinar-se,Médio Oriente,Palácio Presidencial,Polícia Anti-Motim,Primavera árabe,Protestante,Protesto,ViolênciaPhotographer Ed GilesCollection: Getty Images News 2013 Ed GilesCAIRO, EGYPT - FEBRUARY 8: A protester leans against a concrete wall suffering from exposure to tear gas fired by Egyptian riot police during violent protests at the Presidential Palace in Heliopolis on February 8, 2013, in Cairo, Egypt. Protests continued across Egypt against President Morsi and the Muslim Brotherhood two weeks after the second anniversary of the Egyptian Revolution that overthrew former President Hosni Mubarak on January 25, 2011.(Photo by Ed Giles/Getty Images).


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How often does your Outlook go haywire? Or how often does it, uh, stop working?
Yes, I know both mean the same, I just couldn't come up with anything better, that's all. Anyways, if you're having such a tough time with Outlook , maybe it's time you took it to a good computer repair shop. This article wouldn't be of any help!
However, if it's just the Calendar function which is acting up, you might as well fix it with the instructions in this article. Well, let's look at that in the next section. Read on!
The issue
When you try to create a new Calendar entry in Outlook, you might as well get an error message. This error can be a big pain if you don't know how to fix it. But before we get to the Outlook repair steps, how is this caused?
Well, experts think that this error is caused when you've exceeded the .pst size limit. Anyways, the next section will surely help you deal with the error. Go on!
Outlook repair steps
- Try restarting your computer. In most cases, this fixes the issue!
- If that doesn't do the trick, try clearing up the Deleted Items folder in the email client. Open View menu and select Folder List. From here, right click on the Deleted Items folder and choose to Empty Deleted Items Folder. Once that's done, re-launch Outlook and see if it's fixed.
- The Inbox Repair Tool might also be able to help. For this, click Start->Control Panel->Classic View->Mail->Services->Personal Folders->right click and then choose Properties. Once here, make a note of the path information somewhere.
Now click on Start->Accessories->System Tools->Inbox Repair Tool. Type in the path, noted down earlier, under Drives and then click Start to, uh, start off the scan. Once done, re-launch Outlook and see if things are okay.
- If all of the above fail, then you can try creating a new PST file. For this, go to the Services tab (from last step), choose Personal Folders and then click Remove.
Now, click Add and then choose Personal Folders. Click OK and give a suitable name for the .pst file. Open and then press OK.
Well, that should get your Outlook fixed. If not, contact a proper Outlook repair personnel as soon as possible!


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This report aims to help Eastern Europe, Caucasus and Central Asia (EECCA) environmental administrations to harness the potential benefits of on-going public finance reforms in the region.
English, , 693kb
This country assistance program evaluation for Uzbekistan assesses ADB�s assistance, identifies factors affecting ADB's performance, and draws lessons and recommendations for improving ADB's future performance.
English, , 665kb
This report presents the findings of an evaluation of the Railway Rehabilitation Project and the Railway Modernization Project in Uzbekistan.
Russian, , 590kb
The meetings of the Anti-corruption Network for Eastern Europe and Central Asia will take place on 5-8 December at the OECD headquarters in Paris.
English, , 400kb
The meetings of the Anti-corruption Network for Eastern Europe and Central Asia will take place on 5-8 December at the OECD headquarters in Paris.
Russian, , 680kb
Workshop on Green Growth Strategy: OECD Green Growth Strategy from the Perspective of Developing Countries, organised in Seoul, 28 October 2010.
English, , 402kb
The objective of this sector assistance program evaluation is to assess the peformance of the Asian Development Bank assistance in the education sector of Uzbekistan for the period 1996-2009.
English, , 268kb
In 2009 the Black Sea and Central Asia Initiative began to provide policy makers in both regions with economic country-specific analysis.
Black Sea and Central Asia Initiative Co-ordinator Colm Foy presented the Eurasian Competitiveness Programme and Central Asia Inititative at the International Conference "Outcomes of Anti-Crisis Programs and Priorities of Postcrisis Development" in Tashkent on 12 April, 2010.


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- Nigel Sharrocks, the chief executive of Aegis Media Global Brands, is leaving at the end of the summer.
- PHD has elevated Verica Djurdjevic, the managing partner on the Sainsbury's and Macmillan Cancer Support businesses, to managing director, while Frances Ralston-Good, who currently holds that post, will move to the new role of chief innovation officer.
- Bobby Hui named as Arena's new chief strategy officer.
Tags: Aegis Media, Nigel Sharrocks, chief executive officer, CEO, resign, PHD, promotes, MD, managing director, Verica Djurdjevic, Frances Ralston-Good, CIO, chief innovation officer, Bobby Hui, Arena Media, CSO, chief strategy officer, G2 Joshua, executive planning director
Category: People


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PartSelect Number PS2003651
Also known as Thrust Bearing Kit. This kit includes the pulley, washer, thrust bearing, 2 upper race washers and 1 lower race washer. These parts are NOT available separately - they are ONLY available in this kit.
A worn bearing causes slow or no spin.
This part works with the following brands: Whirlpool, Admiral, Estate, Inglis, Kenmore, KitchenAid, Roper, Maytag, Crosley, Jenn-Air, Hardwick, Magic Chef, Amana, Caloric & Glenwood.
This part fixes the following symptoms:
Level of DifficultyEasy
Time to do repair:30 - 60 mins
ToolsNutdriver, Pliers, Screw drivers, Socket set
CustomerJames from Hueytown AL
Lay washer on front, removed belt. Removed center pulley with thrust bearing (pulley was worn on upper end, against thrust bearing, thrust bearing was worn also). Installed new thrust bearing & pulley kit. Cleaned & lubed slots that motor insulators slide in, installed new belt.
Help other customers find the most helpful instructions.
Were these instructions helpful?
Level of DifficultyReally easy
Time to do repair:15 - 30 mins
ToolsNutdriver, Screw drivers, Socket set
CustomerMICHAEL from PLYMOUTH MN
The pump was very easy to replace (3) screws, easy to reach with front panel off. (less than 5 minut job. The brake assembly and thrust bearing was a little harder to do, but not bad at all. I found it best to flip the unit upside down to do the brake assembly. Replaced #10-24 x 1/2" screws with 2" long ones. Remember to do this (1) at a time, then back all (6) off a little at a time to release tesion on the 200lb spring. Swap out parts and tighten back down with 2" screws and then swap them back out for the 1/2" ones (1) at a time. I tipped the unit back on its side to install the bearing & pully kit. This takes some of the weight off of the main shaft allowing you to insert the spacers and split ring. The replacement parts seem to be of eqal or better quality to the originals. Machine runs quiet and smooth.
Level of DifficultyA Bit Difficult
Time to do repair:More than 2 hours
ToolsPliers, Screw drivers, Socket set, Wrench set
Customersteve from lebanon OH
First I unplugged it, then removed the agitator and tub assemblies, then I tipped it back and removed the belt and pully and thrust bearings. After reading on here I learned the brake would stick and after installing the thrust bearing kit I found out I couldnt fit in the shims. That led me to believe the brake was siezed inplace so i couldn't fit in the parts. I removed the thrust bearing kit and removed the transmission assembley. I took apart the brake and found the two pieces had siezed together, after I got them apart I sanded both peices and lubed the shaft with high temp silicone like you would use on a cars brake system. I put the brake back together, BE VERY CAREFUL THE SPRING HAS ALOT OF PRESSURE! I then put the transmission back in, reinstalled the thrust bearing kit and it fit great. I put in the tub and agitator and washed clothes.
ToolsPliers, Screw drivers
Customermarcus from mansfield TX
You do not have to remove any water lines to do this repair. Just unplug the machine from the electric outlet and tip it up on its back to make the repair. I removed the old belt and parts per the instructions. The new kit does not look like the old one. Make sure you mark the minimum and maximum marks with a sharpie on the plastic pulley before intalling it. This will help line up the cam bearing to test the spin cycle. The repair kit says to use the thickest washer but mine would not fit on so I used the thinner washer. Keep the other washer so that in the future if the plastic part wears down and you have spinning problems again you can replace it with the thicker washer. Also make sure you buy a new belt and replace the belt. I loaded it up with a full load of jeans and it works great.
ToolsScrew drivers, Wrench(Adjustable)
Customermichael from mountain home ID
I put the washer on the spin cycle and could see that the tub wanted to move. While it was trying to spin I leaned the washer forward and saw that the motor was spinning and the belt was turning, but the tub wasn't. Did some reading and it was the thrust bearing. I ordered the part and replaced them (very easy, follow the directions that come with the set). After install, the tub would spin.
CustomerEarl from Crest Hill IL
Turned off water suply. Removed drain (pipe is short). Then slid washer out so I could tilt it back and work under the washer. I put a wood brace under the front of the washer for safety. I then followed the instructions that came with the parts.
ToolsScrew drivers
CustomerBrian from South Holland IL
Unplugged and removed the drain and supply hoses from the back; some water will run out so be ready for that. I laid the machine down on two 2x4s to keep from crushing the supply and drain nipples. Just follow the instructions included with the parts, all steps are straight forward accept for two things: 1. they talk about pulling down on the transmission shaft to slip on the retaining clip. With only the end of the shaft sticking out there isn't much to grab on to. I found by using a thin blade scraper inserted into the retaining clip groove, prying downward on the shaft I could then get a regular screwdriver blade into the same groove and expose the entire groove and slip on the clip. 2. they talk about "Adjusting Brake Disengagement" after studying this for a while it just refers to which thickness of thrust washer to use. After trying both the .062 and .032 I found that only the .032 would allow the retaining clip to engage. Rechecking the brake disengagement, everthing seemed to work fine. The machine now has a stronger spin cycle and even bath towels are ready for drying after the normal spin.
ToolsScrew drivers, Socket set, Wrench set, Wrench(Adjustable)
CustomerElmer from Fort Campbell KY
I took out all the tubs and the apparatus holding the gear and thrust bearing assembly. I used a homemade tool (long zipties) to pull the springs off and to put them back on. After that I just replaced the thrust bearing with the new kit and put everything back together.
CustomerRon from Oxford MI
This was easy except for the e-ring which was extremely difficult to remove. When I put the new one on I filed down the bottom of the front edges to make it easier to slide it on. Beware: If you have the washer tipped back against the wall when you do get the e-ring off everything will come sliding off the shaft all at once and go everywhere. These parts are covered with black grease and made quite a mess so you might want to have someone hold them in place so you can gently slide them off one piece at a time.
Level of DifficultyDifficult
ToolsPliers, Screw drivers, Socket set, Wrench(Adjustable)
CustomerDaniel from Sugar Land TX
Tried to remove brake stator with machine tilted back. Thisforced the stator off center. Removed the 6 holddown springs, water level hose and drain hose. Pull drum & transmission out of the case. Placed the drun on the ground, brake facing up. Replaced the short screws on the brake stator with 2" screws one at a time. Caution the spring has 200 lbs of force. Used 2" screws to back off spring. With spring tenshion released replace parts. Reversed proceedurer. Machine quite. Wife is happy.
ToolsNutdriver, Pliers, Socket set
CustomerCarlos from Georgetown TN
i did the repair by the instructions that you had on the web site,and i thank you for all the help you gave me.
CustomerMARK from HEBRON KY
CustomerRobert from South Gibson PA
tip washer up on wooden blocks.remove drive belt.remove "E" clip from tub drive pulley and remove all the parts.Install new parts in kit as per instructions.machine works great!
Time to do repair:1- 2 hours
ToolsNutdriver, Pliers, Screw drivers, Socket set, Wrench(Adjustable)
CustomerSteven from Gilbert AZ
After replacing the tub to pump hose, I adjusted the feet on the washer. Apparently, the main pulley sits so low on these washers that it ground into the floor and stripped out the thrust bearing. I ordered the new thrust bearing clip and put it in the evening I received it. The instructions it came with were very helpful and it was simple to reassemble. Up until the last piece - the retaining clip. The majority of the repair time was spent replacing that clip. If there is a tool that makes the job easier, I didn't have it. I finally got it on with two flathead screwdrivers prying the shaft out while I wedged one corner of the clip in the groove.
ToolsNutdriver, Pliers, Socket set, Wrench set
CustomerJack from Robinson IL
I had diagnosed the problem by checking the resistance at the water inlet electrical terminals and their was no continuity. 1. I shut off the water and removed the lower access/kick panels. 2. Then I disconnected the two wires from the inlet valve terminals noting which one went to which terminal (I'm a bad person since I did not disconnect power from the dishwasher while doing this so it is up to you to decide if the time saved is worth the possible consequences). 3. Using an open end wrench I loosened and disconnected the water supply line. 4. Remove the two screws securing the valve assembly to the inside wall of the dishwasher 5. Loosen the clamp on the water outlet line and slide off the hose. Installation is pretty much the same in reverse. Some times the valve assembly comes with the wire terminals oriented in a different direction than what you may find when you take yours off. If it won't work for you, remove the four screws that hold the electrical side of the inlet valve and rotate it to the needed position. Make sure when putting it back together that you tighten the screws evenly to prevent leaks.
All brand logos are trademarks of their respective owners.
The PartSelect logo is a Registered Trademark of Atlantic Laundry Centres, Ltd.
Copyright © 1999-2015 , Eldis Group Partnership. All rights reserved.


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Android Games
This page contains Cheats for Speed Night organized by sections for Android. This game has "Racing" as genre, made by Icloudzone Inc., released on Apr 23, 2013. If you can't find a hint or secret in our list, then please check this page periodically for the latest updates.
Game Boxshot & Details
- Developer: Icloudzone Inc.
- Publisher: Icloudzone Inc.
- Genre: Racing
- Release: Apr 23, 2013
- ESRB: Not Set
Sorry No Cheats Yet!
If you know cheats, secrets, hints, glitches or other level guides for this game that can help others leveling up, then please Submit Your Cheats and share your insights and experience with other gamers.
Game Questions
Stuck on this game? Set a specific, clearly defined question and let other gamers answer your question or view answers to previously asked questions. If you think you are an expert then please try to help others with their questions.
Ask a Question
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M2.9 Earthquake Shakes Carson
November 6, 2013 10:06 AM
Top Features
CARSON (CBSLA.com) — A magnitude-2.9 earthquake shook the Carson-area Wednesday morning.
The quake hit around 9:42 a.m. and was centered about one mile northeast of the city, the U.S. Geological Survey reported.
There were no reports of damage or injuries.


text:
courtesy: style at home
Sleep Sweetly...
Posted by megan @ a life's design at 1/24/2013
Labels: bedrooms
5 comments:
- ejlynn said...
Very inviting!!Love the one with the large windows and the purple accents!
- January 24, 2013 at 8:01 AM
- Joan Troyer said...
I aslo have a purple and white bed and its so cozy.
- January 24, 2013 at 9:04 AM
- A Perfect Gray said...
oh boy, those were great. I love that b&w blanket in the last image...
- January 24, 2013 at 9:49 AM
- Progresive Home Advantage said...
This site is one of the best I have found :
=> Interior Decorating Ideas <=
=> Interior Design Tips <=
=> Home Interior Decorating <=
Excellent information and facts. Only real difficulty I was basically receiving was viewing the pics. No idea exactly why.
1. Home Insurance Ideas
2. Best Life Insurance Policy
3. Property and casualty insurance
- January 25, 2013 at 4:42 AM
- First House Spouse said...
Ooo, we love that first bedroom photo's wall color! It's just like the blue fox in our master bedroom! Gorgeous captures, thanks!
- February 27, 2013 at 2:40 PM
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Villa Sogno is a Bed and Breakfast built in the '900, just restored, which still maintains high ceilings and floors in Lombard 'terracotta and quarry tiles'.
Most of the furnishings are originally of the house, keeping the atmosphere and taste of that period.
Bed & Breakfast Villa Sogno is situated in a quite area with a nice garden rich with flower beds and fruit trees where our guests can relax.
Our bedrooms are very new and have private bathrooms, showers, hairdryers, air conditioning, TV, your choice of single or double beds, and private entrance to let you have a nice stay.
The Reali family will offer you a rich and delightful breakfast for a nice wake up in the morning.
In B&B Villa Sogno you will taste a little bit of the warm Italian hospitality and we will be happy to give you any kind of information, if desired.
Villa Sogno is 2 minutes far away from Saronno highway and half hour from Malpensa Airport, 8 minutes walking from Train station and 15 minutes by car from the new Milano-Rho International Fair.
|Demandez à un devis
Services Inclues: Lit Bébé, Climatisation, Animaux PAS Autorisés, Salle de Petit Déjeuner, Jardin, Aire de jeux
Services Optionnelles: Location de vélos ( 5,00 par jour)
Check In: 17:00 - 22:30
Check Out: 07:30 - 10:00
*Prix moyens. Après votre demande de disponibilité est envoyé une réponse avec le prix final de votre séjour.


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Report suspected incidents to 0800 321600 (calls are free)
Link to Quarterly Updates of all confirmed incidents in the UK
The Wildlife Incident Investigation Scheme (the Scheme) makes enquiries into the death or illness of wildlife, pets and beneficial invertebrates that may have resulted from pesticide poisoning. The scheme has two objectives:
In practice "companion animals" usually refers to cats and dogs, and "beneficial invertebrates" refers to honeybees, bumble bees and earthworms. Also included in the Scheme are suspect baits, where it is thought that pesticides have been inappropriately applied or used, and spillages of pesticides where this poses a risk to wildlife or companion animals.
The Scheme is essentially a monitoring tool to inform the pesticide approval process. However, where there is clear evidence of a breach of pesticide law enforcement action may be taken.
Cases accepted for further investigation usually fall into one of the following categories:
In some cases pesticides may be found but the origin of the substance is unclear and the cause of death will be unknown or unspecified.
Where poisoning is suspected, a combination of field work, veterinary examination and chemical analysis is used to try to determine the underlying cause of death. The Scheme is operated independently in all four countries of the United Kingdom using the same free phone number given above. Only the reporting of results is co-ordinated and reported here. Those organisations responsible for the investigation and enforcement of incidents are:
In England the Chemicals Regulation Directorate of Health and Safety Executive (HSE) has overall responsibility for the policy of the scheme which is run on HSE's behalf by Natural England. The following pages provide details of the investigation procedures followed by Natural England and the vertebrate and invertebrate flowcharts provide a guide to the reporting and investigation process.
In Wales the scheme is run by the Welsh Government
In Scotland the Science and Advice for Scottish Agriculture (SASA)
and in Northern Ireland the Department of Agriculture and Rural Development
The results of enquiries made by the scheme in the UK are published.
From 2008 all results of enquiries in the UK are published Quarterly in a searchable spreadsheet.
Annual reports for 1998 to 2007 inclusive are available for download.
NC link to vertebrate data sharing added. can you add a link to the comparative assessment page please.
Answers to frequently asked questions regarding the registration and use of pesticides and detergents
Guidance to help those working with plant protection products/pesticides to meet the requirements of legislation
We have a number of databases giving information on Pesticides & Plant Protection Products, including information on Authorisations and Maximum Residue Levels
We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we'll assume that you are happy to receive all cookies on the CRD website. To find out more about the cookies, see our privacy policy.
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The morning coffee run is a daily ritual for many Americans. Whether it's Starbucks, Dunkin Donuts or even just a cup of fresh brewed coffee at home, many have a need for the caffeine fix.
But do you know what brand of coffee gives you the most caffeine per fluid ounce?
Recently the Huffington Post teamed up with the Center for Science in The Public Interest, or CSPI, to analyze nine popular brands of coffee. McDonald's had the lowest amount of caffeine per fluid ounce with 9.1 milligrams.
On the other end of the spectrum was a brand called Deathwish Coffee that contains an apparent 54.2 milligrams of caffeine per fluid ounce. Deathwish Coffee can only be ordered online and costs 19.99 per 12 ounce bag plus shipping.
If you shop around for the coffees in the study that can be store-bought and brewed at home, Peet's Brewed Coffee is the best value.
A 12 ounce bag costs $7.99 at Publix and contains 16.7 milligrams of caffeine per fluid ounce, more than Caribou and Dunkin Donuts, which are pricier.
Seattle's Best is also lower in price at the grocery store, but contains far less caffeine with just 10.4 milligrams per fluid ounce.
Starbucks was the second most caffeinated brand next to DeathWish with 20.6 milligrams of caffeine per fluid ounce, but also the second most expensive to purchase.
Caribou, although popular for his aromatic flavors, was the worst value for the amount of caffeine. It has less then both Peet's and Starbucks but cost more than both at $9.99 a bag.
The CSPI also has a full analysis of caffeine available through its website, including how much caffeine is contained in popular energy drinks and coffee flavored ice creams.


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HOME: North America: Michigan
In search of information and photos on the topic of beautiful Michigan women? Well look no further. We've put together an open message board and image gallery about meeting and dating ladies from the Great Lakes State.
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In accordance with Executive Order 01.01.2007.19, the Code of
Fair Employment Practices, the Department of Labor, Licensing
and Regulation is dedicated to a strong policy prohibiting discrimination
on the basis of age, ancestry, color, creed, marital status, mental
or physical disability, national origin, race, religious affiliation,
belief or opinion, sex, or sexual orientation. Any employee who
believes that he/she has been subjected to discrimination should
report the incident to the Agency Equal Employment Opportunity
(EEO) or Fair Practices Officer, his/her immediate supervisor,
or another appropriate managerial representative. For your convenience,
the names of the office with primary responsibility for equal
opportunity and affirmative action and the Fair Practices Officer
are: Jennifer Dashiell Reed, Director and Fair Practices Officer,
Office of Fair Practices, 1100 North Eutaw Street, Room 214, Baltimore,
Maryland 21201, telephone 410-230-6319.
The State of Maryland is also committed to complying with the
Americans with Disabilities Act. The State will
ensure that qualified individuals with disabilities are given an opportunity to
participate in and benefit from the States services, programs, benefits, and
employment opportunities. Jennifer Dashiell Reed has been designated as the employee
responsible for providing information on reasonable accommodations and for receiving and
investigating any questions or complaints under the Americans with Disabilities Act
against this agency or any of its grantees and representatives. She may be contacted at:
voice (410) 230-6319, TTY (410) 225-7039, Fax (410) 225-3282 or e-mail
firstname.lastname@example.com or by mail at
1100 North Eutaw Street, Room 214, Baltimore, Maryland 21201.
Return to the Office of Fair
Practices home page
Questions or comments regarding the Office of Fair Practices may be directed to firstname.lastname@example.com.
Questions or comments regarding the DLLR website may be directed to email@example.com.
Updated June 9, 2009


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A 6-year-old girl bitten by a water moccasin last week has been released from the intensive care unit and is recovering well at Nemours Children's Hospital, according to her mother.
The poisonous snake bit Kristina Lluesma while she was playing in her backyard with her sister last Saturday at her home in Buenaventura Lakes.
[RELATED: Girl rushed to hospital after snake bite]
Dayana Gonzalez, the girl's mother, said Kristina is doing well. At one point the swelling had spread from the bite near her ankle to her stomach, but Gonzalez said the swelling has almost completely subsided.
The girl also no longer has a fever, which kept her in the ICU for most of the week.
"She was scared. She was crying. She said that she wanted to see [the bite] and I said no you don't want to see it. She was traumatized," said Gonzalez.
Gonzalez said doctors believe her daughter will recover completely after being treated with antivenin. They said it's likely she'll have to spend at least another week in the hospital while doctors care for her leg.
The snake was taken to Reptile World in St. Cloud after it was captured by animal control last week.
Gonzalez told Local 6 after the incident she's even considering moving from her neighborhood dotted with patches of heavy woods.


text: Archived Programs The Race, Gender & Beyond Faculty Development Program The Race, Gender & Beyond Faculty Development Program was a resource for faculty across the university to provide support for teaching for diversity in the form of pedagogical support, curriculum development, and assessment resources. The program is currently not active. The RGB Program offered a range of activities from individual consultations, to drop-in reading groups, to workshops, on-going reading groups as well as summer institutes. The RGB Program offered a menu of options for ways to become involved. For example, some may have found book groups useful, while others preferred recommendations for inclusive teaching resources. Another purpose of the RGB Program was to help faculty form connections through ongoing dialogues about inclusive teaching.


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Daily Tennis News Briefs
is a summary of our full-featured
Daily Tennis News published Monday -
Subscription rates:
Individuals - FREE
CLICK to
Groups - Call for Rates
Bob Larson - PublisherCort Larson - Editor
Bob Larson TennisP.O.
Box 24256Edina, MN 55424 USATel.
Fax 952-920-8940
Visit our websites:
An Executive
Summary of today's Daily Tennis News
© Copyright 2013 Daily Tennis News Wire. All
rights reserved.
Bryans Receive
Persons Of The Year Honors
Bryan and twin brother Mike received trophies from Daily Tennis News
publisher Bob Larson Wednesday as they were recognized as Tennis News
Persons Of The Year 2012.
In a brief ceremony
on court at the BNP Paribas Open in Indian Wells, California, Larson
cited the brothers for their extensive contributions to charitable
events around the world throughout the year.
Azarenkas Physio
Says Injury Is Very Slight
joining Victoria Azarenkas team as touring physiotherapist, Per
Bastholt has been one of the busiest men on the WTA scene. And the Swede
who not too long ago was a staff member on the ATP World Tour maintains
the Australian Open champion has suffered no tendon damage after being
forced to withdraw for her title defense at the BNP Paribas Open in
Indian Wells because of pain in her right ankle.
Berdych Is One Of
The Last Of Old School Czechs
Berdych says hes part of the last generation of Czech players who had
to go through hard times to make it on tour. Berdych was born in 1985,
four year before the fall of the Berlin Wall and seven years before
Czechoslovakia was split into two counties: the Czech Republic and the
Slovak Republic. Like most Eastern European countries after the Eastern
Bloc dissolved, the Czech Republic struggled economically when
transitioning from communism to capitalism, so Berdych didn't have it
Wozniacki Can
Sleep During Lulls
long hours of waiting for a late-starting match is all part of player
life at most events, with the Indian Wells Masters no exception.
Caroline Wozniacki has become an expert at filling her time, with the
former No. 1 big on sleeping whenever possible and at all hours.
Gulbis Is Now The
New Gulbis
win streak may be over for Ernests Gulbis after 13 straight following
his defeat by Rafael Nadal at the Indian Wells Masters. But the Latvian
qualifier who was formerly known more as a tennis playboy than a title
contender is planning to carry on to Miami with his new, serious
approach to his sport.
To receive the full stories,
subscribe to Daily Tennis News -
Daily Tennis NewsBusiness
Larson Tennis produces several tennis newsletters. One of these is Daily
Tennis NewsBusiness. This publication is a daily report on the money in
tennis; Who is getting the money, Where does it come from, What do they
do with it and Where does it go.
If you are interested in the business side of tennis, you will find it
interesting. For a free copy of Daily Tennis NewsBusiness, just send
an email to
email@example.com and say you want a sample of Tennis Business.
Notice Inviting
The City of Coronado,
California, is requesting proposals from qualified individuals or
companies to provide retail tennis merchandise concessionaire services
at the reconstructed Tennis Center.
Proposals may be
obtained at the Coronado Community Center, 1845 Strand Way, Coronado,
CA 92118, by emailing
email@example.com,, on the City of Coronados website,
www.coronado.ca.us, or by calling (619) 522-2451.
Tennis Job of The
USPTA Is Seeking a
Director of Marketing
The USPTA is seeking
a Director of Marketing who will play an important role in expanding the
organizations brand identity. They will seek opportunities for
endorsements and ad sales.
communication and strong relationships with key industry contacts;
managing contracts, ads and general sales; track sales and suggest
promotional ideas; and work on special projects.
The Director will
also have an integral role in planning and coordinating the
associations annual convention, be responsible for organizing and
promoting the trade show and securing endorsements and exhibitors.
Send cover letter and
resume to
email@example.com before March 25.
Pro Tennis Coach
Available: Pro Tennis
Coach. If you want to play the pro tour and youve got what it takes,
call or email me. 952-393-6812 or
Tennis Club For
This is your chance
to own a premier tennis, fitness and swim club located in Augusta,
Georgia. The club is situated on six acres (fee simple ownership),
heavily wooded and beautifully landscaped with palm trees, mature pines
and oaks and other landscape plants, and is located on a tributary known
throughout the world, the famous Raes Creek which runs through the club
and then Augusta National Golf Club, just four miles downstream. The
club features 13 championship courts all of which are fully lighted for
night play. The courts include two Red Clay courts, five Har-Tru clay
courts and six US Open style hard courts (with stands and bleachers set
up for professional tournament viewing). The club also has a junior
Olympic-size swimming pool, and a separate large family pool viewable
from the clubhouse which is shaped like a tennis racquet. The huge
8,000 square foot clubhouse (completed in 2005) includes a potential
fully-equipped restaurant and/or sports bar facility, locker rooms with
nice wood lockers, office space, a spacious pro shop, and complete
nautilus and cardio theatre fitness center. Serious potential
purchasers please request details by email at
email@example.com or call Richard at 706-231-1353.
Tennis Job of the
Tennis Channel, Santa
Monica, California, is seeking a production coordinator. 310 314 9400.
Bob Larsons Tennis
publishes Daily Tennis Jobs newsletter, a daily list of available tennis
jobs throughout the US market. Only $10 a month. For a free sample of
the current list, just email
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Provide Daily Tennis News Briefs to your membership
Contact us at 952-920-8947 for details or email
Indian Wells
Indian Wells
Today's tennis results can be found at
in the right column.
Copyright 2013. No duplication is permitted without permission
from Bob Larson Tennis


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Bathroom Sinks: 10 Beautiful Artistic Sink Designs
Bathroom sinks that make an artistic statement ...
If you're looking for a statement sink, a conversation starter design for your powder room, a guest bathroom or a master bathroom then this fine selection of beautiful bathroom sinks is for you. We've carefully singled out these 10 artistic sinks as the most modern and elegant designs available on the market today. The purpose of this article is to show you what is available in today's modern bathroom sinks. It's up to you to contact manufacturers for details on where to purchase the items mentioned.
Tags: Trendy


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Looking for sauvignon blanc? www.hotfrog.com.au showcases more than 665 businesses grouped by sauvignon blanc within Australia. To find more businesses related to sauvignon blanc, Wine or Shiraz, and to filter by locality, use the left navigation menu.
Karridale, WA
KarriBindi is a family owned and operated vineyard with 32 hectares of grapes under vine. The KarriBindi vineyard is located in Karridale, a premium …
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Melbourne, VIC
Great wines from iconic Australian wine regions. We source the best fruit available to bring you affordable wines in edgy packaging - far removed fr…
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Home, Sabotage Wines Australia, emerging Mornington Peninsula based Victorian winery producing highly textured sauvignon blanc, riesling and pinot noir
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Moonambel, VIC
Lalla Gully Sauvignon Blanc: Single vineyard premium varietal from north-east Tasmania. Very limited production. Lalla Gully Chardonnay: Unique blend from Tasmania and Victoria, made in the Burgundian tradition, very li…
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Our vineyard has Sauvignon Blanc, Semillon, Merlot, Cabernet and Shiraz all planted in 1998
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Limbic Wines, Established in 1997, Premium Wines, Sauvignon Blanc, Pinot Noir, Chardonnay, Jenny & Micheal Pullar
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McLaren Vale, SA
Renards Folly - Premium Award Winning McLaren Vale Shiraz, Sauvignon Blanc Semillon & Sangiovese Cabernet
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Casas Vineyard & Estate: Producers of fine Cabernet Sauvignon, Shiraz, Sauvignon Blanc & Chardonnay Casas Wines has now ceased trading
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The Mount Trio vineyards are in the Great Southern region of Western Australia. High quality, affordable wines including savignon blanc, riesling, semillon sauvignon blanc, chardonnay, pinot noir, cabernet merlot and sh…
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Tanunda, SA
Marschall Groom Cellars is a very small family winery situated in the famous Barossa Valley region of South Australia. The winery produces three varietals, Sauvignon Blanc, Shiraz & Zinfandel - under the label GROOM
Penola, SA
Raidis Estate is a family owned and operated winery in Coonawarra, Australia. Their hands-on approach and low-yielding vines produce exceptional Cabernet Sauvignon, Riesling, Pinot Gris, Sauvignon Blanc
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Dixons Creek, VIC
…with wine makers with vineyard and cellar door located in Dixons Creek, right in the heart of Yarra Valley wine country. Graeme Miller Wines produce a first class Australian Shiraz, Chardonnay, Sauvignon Blanc and Rose
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Brighton, VIC
Snobs Creek offers a diverse range of wine varietals such as Chardonnay including Reserve Grade, Roussanne Viognier, Pinot Gris, Dolcetto, Pinot Noir including Reserve Grade, Sauvignon Blanc and more
Pinot NoirWhite WineShirazVineyard
Griffin Wines situated in the Southern Adelaide Hills, South Australia at Kuitpo is a family owned estate cool climate wine label that specialises in hand made Shiraz, Pinot Noir, Merlot, Chardonnay and Sauvignon Blanc
Pinot NoirMerlotChardonnayCool Climate Wine
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Griffith, NSW
… Sample from a wide range of Westend's excellent award winning wines, from the '2009 White Wine of the Year – Richland Sauvignon Blanc' to Westend's flagship wine – Three Bridges Durif. Open seven days a week for tastings.
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Ventnor, VIC
We produce Chardonnay, Sauvignon Blanc, Merlot, Pinot Noir, Rose, Cabernet Sauvignon, Cabernet Merlot and Shiraz, as well as a very special Noble Riesling dessert wine Here, on the tranquil side of the island,…
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Elwood, VIC
We are a Melbourne based online wine store who endeavor to find the best wine, from the best wineries, at what are legitimately the best prices.
We've personally built a rapport with these winemakers, building undenia…
Semillon Sauvignon BlancRieslingPinot NoirMerlot
Pokolbin, NSW
…Hunter favourites as well as niche varieties. Currently producing Chardonnay, Chambourcin, Verdelho, Merlot, Sauvignon Blanc, Tannat, Shiraz and Pinot Noir grape varieties the vineyard is capable of producing a wide…
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Yarra Glen, VIC
…Valley Wine Show. Ranges include Yarrawood Tall Tales, Yarrawood Yarra Valley. Wine varietals include: Sauvignon Blanc, Verdelho, Chardonnay, Autumn Harvest Riesling, Botrytis Riesling Rosé, Pinot Noir, Merlot,…
Online Wine ShopVictorian WinesAward Winning Winespremium wines
Sydney, NSW
Liquor store specialising in corporate sales of wine,beer and spirits. FREE Sydney delivery for orders over $100. Massive range of wines, beers as premium spirits including Asian spirits. Open late 7 days a week.
Pinot NoirWhite Winecorporate wineRed Wine
Northbridge, NSW
We offer you great value wines at low cost because we keep our overheads down. We get a Master of Wine to select all our wines so you know every bottle will be a great one! Follow three easy steps to buy wine online 1. …
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Pyrmont, NSW
At Cracka wines we reckon that
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Waikerie, SA
Mirabella Vineyards is a winery which encompasses 5 generations of family tradition. Mirabella Vineyards is a boutique winery in the Riverland has vines up to 80 years old, producing top quality fruit and premium qualit…
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North Boyanup, WA
…Frog Wines have also just released their first white wine, the thoroughly enchanting Foreigner a Semillon Sauvignon Blanc Blend. Bonking Frog wines and gift lines are available online and at their cellar door. And the…
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Cranbrook, WA
Our signature Riesling and Tunney Cabernet Sauvignon support our range of wines that include Sauvignon Blanc, Chardonnay, Katies Kiss Sweet Riesling, Merlot and Shiraz
Victoria, NSW
Find best online wine deals on Just Wines Australia. Just Wines offer you to order online wines at Melbourne, Sydney, Perth, Adelaide and surrounding areas with affordable prices, Fast Delivery. Buy red wine, white wine…
Pinot NoirOnline Wine ShopMail Order WineBuy Wine
Our Sauvignon Blanc holds the juiciness of fresh encounters with the familiarity of a continued conversation When we set out to create our blend we reflected on some of Australia's greatest pioneering wines …
Preston, VIC
Wine Online is the best Australian wine web store. We provide wine at a whole new level - and that is online. We have a pretty simple web page - so you won't struggle to find your wine and make the purchase. We are comm…
Pinot NoirWhite Winewine onlineonline wine
Campbelltown, NSW
Fine Australian wine manufacturers and distributors around the globe. If you are a wine wholesaler, distributor who is looking for great wines from a great wine making country, then contact us by visiting google, or vie…
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Narre Warren East, VIC
…cottage accommodation sleeping up to six people. Buy Three Wise Men Shiraz and Three Wise Men Pinot Noir, Sauvignon Blanc Semillon and other fine Australian wine. Functions by arrangement. Wines available for purchase…
Artarmon, NSW
…terroir as they know if it is healthy then the vines will be able to produce good fruit. Grapes grown include Sauvignon Blanc, Pinot Gris, Riesling, Pinot Noir, Syrah, Cabernet Sauvignon, Cabernet Franc, Merlot,…
Rieslingcabernet merlotPinot NoirSemillon
Narrabeen, NSW
At WineXpress, we hope not only to give you the experience of enjoying fantastic wines from small boutique wineries, but also to help these devoted and sometimes struggling winemakers to survive in what has been widely …
Pinot NoirChardonnayNew Zealand Wines cabernet
We are a Designated Drivers business, What we can do for you is drive your vehicle to your destination of your choice, and once you have used us, you will have peace of mind that your vehicle is at your destination with…
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Myrrhee, VIC
Toombullup North Winery is located within the King Valley wine region, located in North East Victoria. Toombullup North Winery specializes in Super Premium table wines with varieties including Arneis, Sauvignon Blanc, C…
Pinot NoirMerlotcabernetWhite Wine
East Sydney, NSW
Buy Wine Online | Buy Good Wines | Wine of Australia : - Red Wine White Wine Sparkling Wine Sauvignon Blanc ecommerce, open source, shop, online shopping
Pinot NoirWines DirectChardonnayCabernet Sauvignon
NZ Wine Online is an Australian based e-store that offers customers a range of New Zealand Wine at low prices. This range of New Zealand Wine includes Chardonnay, Pinot Gris, Gewürztraminer, Syrah, Sauvignon Blanc, Cabe…
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Murrumbateman, NSW
…grown fruit. Barton Estate's premium wines include Petit verdot, Shiraz, Sangiovese, a Cabernet blend comprising all five of the great grape varieties of Bordeaux, trophy winning Riesling and Sauvignon blanc Semillon
Semillon Sauvignon BlancRieslingSangiovesecabernet
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|Unlocking the Athlon II X3 435
|Written by Michael Schuette
|Nov 27, 2009 at 05:23 AM
Despite having different code names such as Toliman, Heka and Rana, AMD's triple core solutions are anything but a discreet die design. Rather, existing Deneb (Phenom II X4) and Propus (Athlon II X4) dies are tested and those with defects in one of the four cores are rebadged as triple core solutions. It is no secret though, that often enough, marketing and demand will create defective dies where the only defect is realistically the lack of the latter. In other words, many of the triple core CPUs offered at a budget price compared to their full-fledged brethren are wolves in a sheep skin.
To make matters more interesting, it turns out that a number of the Athlon II brand processors supposedly based on the Propus core are in fact Deneb processors featuring the 6 MB shared L3 cache, a feature that makes those CPUs more pliable for gaming purposes. The good news in this case is that the crippling inflicted by AMD appears to be done on the basis of register programming rather than blowing fuses and, therefore, with a few BIOS tricks, it may be reversible. To sum it up, chances are that an inexpensive Athlon II X3 processor may transform into a Stanley Beamish-like Phenom II X4 with the extra bonus of the pill not wearing off.
BIOS screenshot of the Athlon II X3 435 after enabling "Unleashing Mode"
The big question is, as always, is it going to work or not. The short answer is: maybe! As long as yields are good enough so that there is no fall-out of bad cores and demand for the triple core processors by the OEMs his high, chances are that many of the X3 435 and similar are going to be either fully functional Athlon II X4 or even Phenom II processors.
The Test:
We started out with a sample of the Athlon II X3 435 (media sample) and running it on the ASUS M4A79T using the 2002 (or later) BIOS revision (the latest we tested was the 2205 version).
In the "Advanced" menu of the CMOS Setup utility, select Advanced Core Calibration (ACC) and change it from "Disabled" to "Auto". This setting will unhide a separate tab called "Unleashing Mode" with the options "Enabled/Disabled". When "Enabled" is selected, it will give several options for triple core selection and, more importantly, it will unlock the L3 cache. A shortcut is provided by pressing "4" during POST to enable all four cores. Unfortunately, the ASUS BIOS does not allow to selectively enable all cores without activating the L3 cache.
Default configuration of the test CPU.
Did it Work?
To make a long story short, the sample we had could not be persuaded to make it into Windows7, regardless of which core we disabled. As soon as the "L3" cache was enabled, the system managed to get to the "Windows7"/"Previous OS" screen but from there on, all we got was a black screen. We tried to underclock the CPU by selecting multipliers as low as 5x to give a marginal core a chance to at least make it into Win7 at 1 GHz, to no avail.
At this point, it is not clear whether the "Unleash Mode" (CPU Unlock in MSI parlance) just misreports a non-existent L3 cache, which then causes the sytem to stall during boot or whether the L3 on our sample was actually present but crippled so badly that the CPU just couldn't make it into Windows. It doesn't matter, though, this is one sample and whether we have a true Propus die or whether it is a Deneb in disguise is inconsequential. What matters is that there is a potential for getting a bargain deal and there have been reports on Inpai.com.cn that at least some of the retail processors in circulation are in fact Phenom II CPUs.
We confirmed this morning with AMD that the '435 sample we have in our hands is in fact a Deneb die. We also tried to down-clock the NB/L3 all the way to 1200 MHz to see whether this would allow to get into Windows but no matter what, the system kept stalling immediately after launching the boot process, indicating that the die was defective enough to not even work at low frequency.
What we don't want to do with this article is sending our readers on a wilde goose chase, trying to hunt down any possible data code of Athlon II X3 and potentially getting frustrated by lack of success. What we do want to do, though, is encourage those who settle for the X3 435 to try their luck and see how far they can get. Hey, you never know...
Special Thanks to our Reader "Justapost" for pointing us into the right direction.
|Last Updated ( Dec 06, 2009 at 12:55 PM )
|<Previous Article
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Daylight Lamps is a new website by Wye Needlecraft Ltd which is a multi-award winning shop based in Bakewell in the heart of the Peak District in Derbyshire. The shop has been open since 1992 and has sold the Daylight range from the begining.
We have many more innovative and exciting plans to develop our business. Our ambition is to keep growing our company while staying true to our customers who are the important bit that make us who we are!
Your comments are always welcome, whether you have a suggestion, a comment on our website, an item you think we should be stocking that you can't find anywhere and so on - just let us know and we'll do our best!
Best regards


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Kristin Chenoweth on Her Weird Blind Date Experience on 'Late Late Show' (VIDEO)
His idea was to pass cute notes back and forth, culminating in him asking Chenoweth to take off her panties and give them to him. Then, when she does they adorably have "I Love" and then his name on them. Funny stuff, right? Well, Chenoweth wasn't down with that.
Okay, so he got shot down. What do you do next? Well, this guy decided that he didn't want to give up on the dream, so he showed up with his own pair of panties for her to use should she change her mind. Which means he either just happens to have panties lying around that say "I Love" and then his name on them, or he went out and got these for this joke she's already rejected.
Now picture the guy watching this interview, as well as the mutual friends. We think there's going to be at least one conversation after this episode airs.
- See a great moment on TV? Send feedback & suggestions to firstname.lastname@example.com
- New clips post all day long. Don't miss out, subscribe to our RSS feed


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FBC has many opportunities for middle and high school students.
Youth Group
If you're in 6th-12th grade, join us on Sunday nights to learn something about God's word, about yourself, and just as important – to have fun! Get more info at the Youth Group page.
Sunday School
Join us downstairs at 9:30AM for one of our pre-worship classes.
2013/2014 Youth Classes
Middle School — Mrs. Amy Veling
High School — Mr. Paul Helgesen
Mission Trips/Service Opportunities
The FBC Youth Group (and the church as a whole) has a goal of impacting the communities nearby, as well as impoverished countries worldwide. With this in mind, students have many opportunities to help out at local food banks and shelters. A longer service trip is usually scheduled for the summer. See examples of past trips on the FBC Youth Missions page!


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Printed with a fusion of tropical patterns, Just Cavalli's satin shorts are a statement-making style. The loose fit and adjustable waist ensure a comfortable fit. This breezy pair will work in the city or on vacation - just add a simple blouse and bold sandals.
Multicolored printed satinSlant pockets, curved hemTies at elasticated drawstring waist100% polyesterMachine wash
Product code: 340545 - Need help? Contact us


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Brittney Skye Is The Best Friend A Cock Can Have
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Starring Brittney Skye, Mark Ashley and Marty Romano, DVD: Passion Designer
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Busty Brittney Skye spends an afternoon giving blowjobs and taking two big cocks in her pussy
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Contamination Suppression in Chemical Fluid Deposition
This invention relates to methods for depositing materials onto a substrate surface or into a porous solid. Thin filsm of materials such as metals, semiconductors, or metal oxides insulators are of great importance inthe micro-electronics industry. Fabrication of integrated circuits involves formation of high purity thin films, often in multiple layers, on patterned substrates, commonly using chemical vapor deposition.
The invention is based on the discovery that contamination, e.g., oxidation, of material deposited onto a substrate surface or into a porous solid can be suppressed through the appropriate selection of a material precursor, delivery agent (e.g. solvent), reaction conditions (e.g., temperature), and/or presence of additioal agents.
See the link to the PDF of the Patent below.
Production of Thin Films for micro-electronics
Produces high purity thin films at temperatures lower than conventional Chemical Vapor Deposition (CVD) temperatures.
Patent US 6,984,584 issued
See Patent Link Above
Ling X. Shen, Ph.D., M.B.A.
Licensing Officer
Commercial Ventures and Intellectual Property
Phone: 413-545-5276
E-mail: firstname.lastname@example.com


text:
Food Prices to Double by 2030
In the Near Future, Price of Food Will Drastically Increase
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To solve the situation, Oxfam is recommending a complete overhaul of the world's food system. The chief executive said, "We are sleepwalking towards an age of avoidable crisis. One in seven people on the planet go hungry every day despite the fact that the world is capable of feeding everyone. The food system must be overhauled." It's easy to call for action, but the change that is necessary will probably not come quick enough. Are you concerned about a global food crisis?
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