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Real turn focus to trimming squad
Wednesday 05 August 2009
Real Madrid have ended their search for new signings with the deal to land Spain midfielder Xabi Alonso from Liverpool and will now focus on trimming their squad, director general Jorge Valdano said today.
Alonso flew into Madrid in the morning and passed a medical, Real said on their website (www.realmadrid.com), ahead of completing a reported 30 million euro transfer.
He is expected to train with the rest of the squad this evening, before joining the club's tour to North America which leaves on Thursday.
"We understood Xabi Alonso was an important player to complete our squad, and it was worth Real Madrid fighting for him," Valdano told radio station Cadena Ser.
"The club needed a shake up. Some very good players have come in to strengthen every position and the fans are very excited.
"With this move we end the search for new players. We continue searching for an exit for those who are surplus to requirements."
Having spent around 250 million euros ($359.8 million) to lure eight players including Cristiano Ronaldo, Kaka and Karim Benzema to the Bernabeu, Real have until the end of August to cut Manuel Pellegrini's squad back to 25 players.
Midfielder Rafael van der Vaart and forwards Klaas Jan Huntelaar and Alvaro Negredo have been linked with interest from clubs in Germany, Italy and England.
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Hop Growing / Re: Thinking about growing hops« on: May 18, 2015, 07:48:12 AM »
We have hundreds of deer where I live and they will eat anything. The only thing they wont eat are the hops. One of them tries every year; I always find a cone or two and a leaf with a bite taken out, but they must taste pretty bad if even the deer won't eat them.


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Headlines November 21, 2013 Full Show | First Story >
U.S. Troops Granted Immunity in Afghan Security Pact
The White House and Afghanistan have reached agreement on a bilateral security pact that would preserve a U.S. military occupation beyond 2014. Under the deal, the U.S. military would maintain several bases, and its troops would be immune from prosecution in Afghan courts. The United States would not conduct military operations "unless mutually agreed." Afghan officials say they have kept their demand for a White House apology over deadly raids on Afghan homes. But in Washington, Secretary of State John Kerry said an apology is off the table.
Secretary of State John Kerry: "But let me be clear: President Karzai didn't ask for an apology. There was no discussion of an apology. There will be — there is no — I mean, it's just not even on the table. He didn't ask for it; we're not discussing it. And that is not the subject that we have been talking about. What we've been talking about are the terms of the BSA [Bilateral Security Agreement] itself."
The U.S.-Afghan pact now goes before an Afghan council of elders, known as a loya jirga, meeting in Afghanistan today. If approved, the United States is expected to maintain a force of around 8,000 to 12,000 troops after the formal withdrawal next year.
U.S. Launches Drone Strike in Pakistan Despite Reported Halt
The United States has carried out a new drone strike in Pakistan despite a reported pledge to halt the attacks during peace talks with the Taliban. U.S. missiles struck a religious school in northwestern Pakistan earlier today, killing up to five people. The strike comes one day after Pakistan's foreign minister said the United States had promised to hold back on drone strikes while the Pakistani government tries to engage the Taliban in negotiations. Pakistan accused the United States of derailing peace talks earlier this month after a strike that killed Taliban chief Hakimullah Mehsud.
Dozens Dead in Iraq Bombing
At least 25 people have been killed and more than 30 wounded in a car bombing in northeastern Iraq. It was the third major bombing in Iraq this week. Iraq is currently suffering its worst violence in five years.
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The news comes amidst a continued diplomatic fallout between Indonesia and Australia over National Security Agency spying. Indonesia has recalled its ambassador to Australia and frozen military cooperation following recent disclosures showing the NSA used Australia as part of its global spying operations. Australian intelligence agencies reportedly tried to tap the phone of Indonesia's president and other top officials. Earlier today, around 200 people marched on the Australian embassy in Jakarta, some burning Australian flags. During a visit to Washington, both Australian Foreign Minister Julie Bishop and Secretary of State John Kerry refused to comment on the Indonesia controversy.
Australian Foreign Minister Julie Bishop: "We do not discuss intelligence matters, certainly not allegations. We do not discuss them publicly, and we will not do so."
Secretary of State John Kerry: "We work with our friends in Indonesia on many different issues, and we will continue to do that. But whatever has been or not been released or being discussed in the papers, I believe, as I think our friends in Australia do, is a matter of intelligence and intelligence procedures, and we don't discuss intelligence procedures in any sort of public way."
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Virden Community Arts Centre presents The Travelling Mabels
Thursday, March 21, 2013
Theatre & Arts
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Tags » Captain Hook
101 Words of Wisdom Day Fifty-Five: Living in Crisis
If you're always waiting for everything to be perfect, you'll never truly live.
Share your own words of wisdom or thoughts on the words posted below, on the Facebook page or or tweet to me at @WriteForBoots using the hashtag #WordsOfWisdom101.
Story Spotlight: Two Princes
Title: Two Princes
Author: Scheherazade06
Fandom: Once Upon a Time
Rating: T
AU/Canon/AH: AU
Complete? yes
Summary: Inspired by the song. AU: Princess Emma was raised by her birth parents, but a curse on her 16th birthday sends her to Neverland. 217 more words
Once Upon A Time...
Once Upon A Time…
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CABIN1 acts as a negative regulator of p53 by keeping p53 in an inactive state on chromatin. Genotoxic stress causes rapid dissociation of CABIN1 and activation of p53. However, its molecular mechanism is still unknown. Here, we reveal the phosphorylation- and ubiquitination-dependent degradation of CABIN1 upon DNA damage, releasing p53 for transcriptional activation. The DNA-damage-signaling kinases, ATM and CHK2, phosphorylate CABIN1 and increase the degradation of CABIN1 protein. Knockdown or overexpression of these kinases influences the stability of CABIN1 protein showing that their activity is critical for degradation of CABIN1. Additionally, CABIN1 was found to undergo ubiquitin-dependent proteasomal degradation mediated by the CRL4DDB2 ubiquitin ligase complex. Both phosphorylation and ubiquitination of CABIN1 appear to be relevant for controlling the level of CABIN1 protein upon genotoxic stress.
CABIN1 was initially identified as a calcineurin-binding protein acting as a negative regulator of both calcineurin and MEF2 (myocyte enhancer factor 2) (1,2). Numerous reports have thoroughly elucidated the mechanism of MEF2 repression, demonstrating that CABIN1 brings a huge complex of repressors including histone deacetylases (HDACs) and histone methyltransferase (HMT) (3–7).
We recently showed that CABIN1 plays a pivotal role in p53-dependent gene regulation by occupying the promoters of a subset of target genes with p53 as a repressive regulator in the unstressed condition (8). Our previous research provides an explanation for p53 occupancy on target promoters without activating gene expression (9–11). This study also gives rise to the necessity of CABIN1 dissociation from p53 upon genotoxic stress for activation of the target gene expression.
In response to genotoxic stress, eukaryotic cells activate conserved pathways that increase expression of many genes involved in cellular functions such as DNA repair, cell-cycle arrest and cell death (12–14). Protein kinases ATM (ataxia-telangiectasia, mutated) and ATR (ATM and Rad3-related) are emerging as potential sensors of DNA damage. Activated ATM and ATR phosphorylate downstream effector kinases including CHK1 (checkpoint kinase 1) and CHK2 (checkpoint kinase 2) for the damage-signaling cascade (15,16). ATM and ATR share consensus sites, the Ser-Gln (SQ) and Thr-Gln (TQ) motifs, and CHK1/CHK2 recognize the R–X–X–S/T motif. Moreover, CABIN1 is reported to have a putative ATM-/ATR-mediated phosphorylation site in response to UV irradiation (17). This fact prompted us to examine the possibility of CABIN1 phosphorylation upon DNA damage.
DNA-damage-binding proteins (DDB1 and DDB2) are subunits of a heteromeric complex, which is known as the primary detection device for UV-induced lesions in the genome and mediates global genome nucleotide excision repair (GG-NER) (18–20). The CRL4DDB2 ubiquitin ligase complex participates in diverse cellular and physiological processes including DNA repair, DNA replication and chromatin remodeling. More specifically, the ligase complex facilitates NER by targeting XPC and histones H2A, H3 and H4 for ubiquitination (21–24). The complex also targets the replication licensing factor, CDT1, for degradation which in turn results in delayed cell-cycle progression, finally permitting time for DNA repair (25).
Here, we found that ATM and CHK2 mediate phosphorylation of CABIN1 and the CRL4DDB2 ubiquitin ligase complex binds and mediates CABIN1 ubiquitination, leading to proteasomal degradation upon DNA damage. These findings provide an explanation of prompt activation of bound-p53 on promoters upon DNA damage.
HEK293 and HCT116 cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% (v/v) fetal bovine serum, 50 U/ml of streptomycin and penicillin (Invitrogen). Reagents, including puromycin, polybrene, cycloheximide, doxorubicin, etoposide, caffeine and CHK2 inhibitor II, were purchased from Sigma-Aldrich, Inc. MG132 was purchased from Calbiochem.
Various CABIN1 expression vectors were described previously (7). Mammalian expression vectors coding for human DDB1 and CUL4A were obtained from Addgene (Cambridge, MA, USA). The expression vectors for full-length DDB2 were generated by inserting DDB2 PCR fragments from pOTB7-DDB2 (obtained from 21C Frontier Human Gene Bank, Daejeon, Republic of Korea) into pcDNA3-HA. The plasmid pcDNA4/HisMax-ubiquitin was generously provided by Prof. C.H. Chung (Seoul National University, Republic of Korea).
For lentiviral-mediated RNA interference, we purchased pLKO-DDB1, DDB2 and CABIN1 from Open Biosystems, pLV-ATMi and pLV-ATRi from Addgene (Cambridge, MA, USA). Lentiviruses were produced according to the manufacturer's protocol using the BLOCK-iT Lentiviral RNAi expression system (Invitrogen). Briefly, 293FT cells were transfected with the pLKO shRNA vector in combination with packaging vectors using Lipofectamine 2000 (Invitrogen). The virus containing supernatant was collected and used for target cell infection. Forty-eight hours after lentiviral infection, puromycin was added for stable cell generation. To develop the adenoviral DDB2 expression system, we used Gateway Cloning kit (Invitrogen). Briefly, DDB2 PCR fragments from pOTB7-DDB2 were subcloned into pENTR3C and recombined with pAd/CMV/V5-DEST using Gateway LR Clonase II (Invitrogen). The production and amplification of adenovirus were performed as described previously (26).
To prepare the whole-cell extracts, cells were lysed with TETN buffer [50 mM Tris–HCl (pH 7.5), 150 mM NaCl, 1 mM ethylenediaminetetraacetic acid (EDTA), 1% (v/v) Triton X-100, protease inhibitor cocktail (Roche) and 1 mM PMSF]. Cell lysates were incubated with the indicated antibody and protein G beads (Santa Cruz Biotechnology). Immunoprecipitates were washed three times with the same lysis buffer and boiled with sample loading buffer for sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). The following antibodies were used: FLAG (M2 antibody, Sigma-Aldrich), HA (16B12, Covance), MYC (9E10, Covance), β-actin (Sigma-Aldrich), p53 (DO-1, Santa Cruz Biotechnology), DDB1 (BD Transduction Laboratories), DDB2 (Santa Cruz Biotechnology), Ubiquitin (P401, Santa Cruz Biotechnology), ATM (2C1, GeneTex), CHK2 (ab8108, Abcam) and CABIN1 (polyclonal antibodies were generated by AbFrontier).
Cells transfected with Myc-tagged CABIN1 and His6-tagged Ubiquitin for 24 h were harvested and re-suspended in Buffer A (6 M guanidine–HCl, 0.1 M Na2HPO4/NaH2PO4, 10 mM imidazole at pH 8.0). The lysates were then sonicated before adding 50 μl of Ni–NTA agarose and rotating at room temperature for 4 h. The beads were washed with Buffer A, Buffer A/TI [1 vol. of Buffer A, 3 vol. of Buffer TI (25 mM Tris–Cl, 20 mM imidazole at pH 6.8)] and Buffer TI. To the washed beads, 50 μl of His-ubiquitin elution buffer [0.2 M imidazole, 5% (w/v) SDS, 0.15 M Tris–Cl at pH 6.8] was added and incubated for 20 min at room temperature. The eluted ubiquitin conjugates were boiled with SDS sample buffer and subjected to western blot analysis.
A stable cell line expressing FLAG-tagged CABIN1 was generated and used for CABIN1 purification. Briefly, cells were lysed with TETN lysis buffer and soluble lysates were incubated with anti-FLAG-M2-agarose (Sigma-Aldrich) for 2 h. Precipitates were washed four times with lysis buffer and eluted with 50 μl elution buffer [50 mM Tris–HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 0.1 μg/μl FLAG peptide (F4799, Sigma-Aldrich)].
HEK293T cells were transiently transfected with pcDNA3-FLAG-ATM using polyethylenimine (PEI, Polysciences). Forty-eight hours after transfection, the cell lysates were prepared and immunoprecipitated with anti-FLAG antibody and protein G beads. Precipitates were washed and subjected to ATM kinase assay as described previously (27). For CHK2 kinase assay, the purified recombinant full-length human CHK2 kinase (#7434, Cell Signaling Technology) was used following the manufacturer's protocol.
Chromatin immunoprecipitation (ChIP) assays were performed as described previously (7,8). Primers used for GADD45 were: forward, 5′-AGCGGAAGAGATCCCTGTGA-3′ and reverse, 5′-CGGGAGGCAGGCAGATG-3′.
Total RNA was extracted using TRIzol reagent (Invitrogen) and reverse transcribed into cDNA as described previously (28). Quantitative real-time PCR was performed with the SYBR Green qPCR kit (Finnzymes, F-410L). The primers for real-time PCR were as listed previously (8). Primers used for DDB1 RT–PCR were forward, 5′-GGCAAAGACCCCAACACTTA-3′ and reverse, 5′-CGTGCTATTGATGCTGGCTA-3′. Primers used for DDB2 RT–PCR were forward, 5′-TCCTCTACTCGCTGCCGCAC-3′ and reverse, 5′-CTGGGTATCGGCCCACAACA-3′.
We previously reported that CABIN1 acts as a negative regulator of p53 by co-occupying a subset of p53 target promoters in the absence of genotoxic stress (8). Under the genotoxic stress condition, CABIN1 should be dissociated from those promoters not to disturb p53 target gene expression. To determine the CABIN1 protein level in response to genotoxic stress in this study, HCT116 cells were treated with either doxorubicin (0.4 μg/ml) or etoposide (10 μM) and were subjected to immunoblot analysis. The protein levels of CABIN1 decreased in a time-dependent manner after the treatment of DNA damaging agents or UV irradiation (Supplementary Figure S1 and Figure 1A). To investigate whether the decrease in the CABIN1 protein level was proteasome-mediated, HCT116 cells were treated with the proteasome inhibitor MG132 prior to UV irradiation. Treatment of MG132 prevented the decrease in the CABIN1 protein level after UV irradiation (Figure 1B). These results implied that CABIN1 degradation involved CABIN1 ubiquitination. Thus, we transfected HEK293 cells with expression vectors of Myc-tagged CABIN1 along with HA-tagged ubiquitin. Cells were treated with MG132 for 16 h and then subjected to immunoprecipitation followed by immunoblotting with the indicated antibodies. The Myc-tagged CABIN1 protein exhibited smearing patterns in cells expressing exogenous ubiquitin and such protein patterns were enhanced by MG132 treatment (Figure 1C). In addition, his-ubiquitin pull-down assays were performed to confirm CABIN1 ubiquitination (Figure 1D). To investigate the role for DNA damage in stimulating CABIN1 ubiquitination, ubiquitination assay was performed with or without UV irradiation and the results showed a significant increase of CABIN1 polyubiquitination (Figure 1E).
In response to UV irradiation and other genotoxic treatments, cells undergo DNA damage checkpoint response including a phosphorylation-based signal transduction cascade. To gain some insight into the mechanism leading to rapid degradation of CABIN1 protein upon DNA damage, we tested whether checkpoint kinases mediate the cue signaling of CABIN1 reduction. HEK293 cells were transfected with the FLAG-tagged ATM and the Myc-tagged CABIN1 expression vector and were subjected to immunoprecipitation assay to test the physical interaction of ATM and CABIN1. Myc-tagged CABIN1 readily co-immunoprecipitated with FLAG-tagged ATM (Figure 2A). To examine whether CABIN1 can be directly phosphorylated by ATM in vitro, the full-length FLAG-tagged CABIN1 protein, purified from a stable cell line expressing FLAG-tagged CABIN1, was used as a substrate in an in vitro kinase reaction with immunoprecipitated ATM from FLAG-ATM transfected HEK293 cells (Figure 2B). Purified CABIN1 protein was strongly phosphorylated by the immunoprecipitated FLAG-ATM wild-type (Figure 2C). We also performed binding assays with CABIN1 and other checkpoint kinases. Co-IP assays revealed that CHK2 was readily bound to CABIN1 (Figure 2D), whereas ATR and CHK1 did not (Supplementary Figure S2). To establish the functional relevance of CHK2/CABIN1 interaction, we tested whether CABIN1 is phosphorylated by purified CHK2. Phosphorylation of full-length CABIN1 was increased by adding of CHK2 kinase (Figure 2E). These results demonstrate that CABIN1 is a bona fide substrate of DNA damage-activated checkpoint kinases, ATM and CHK2.
To investigate the effect of these kinase activities on CABIN1 degradation, HCT116 cells were treated with an ATM/ATR inhibitor, caffeine and the protein level of CABIN1 was observed by immunoblot assay before and after UV irradiation. Caffeine blocked the reduction of the CABIN1 protein level after UV irradiation (Figure 3A). We next generated lentivirus expressing short-hairpin RNAs (shRNAs) which have specific sequences targeting ATM. Knockdown of ATM resulted in a higher level of CABIN1 protein even after UV irradiation in comparison with control (Figure 3B). We also investigated whether CHK2 contributes to the degradation of CABIN1 protein, using a specific inhibitor of CHK2 kinase activity and by shRNA-mediated CHK2 knockdown. Interestingly, both approaches markedly blocked the reduction of CABIN1 protein in response to UV irradiation (Figure 3C and D).
To verify that the activity of ATM and CHK2 affected CABIN1 ubiquitination, we transfected HEK293 cells with the Myc-CABIN1 expression vector in combination with HA-ubiquitin and FLAG-ATM or the FLAG-CHK2 expression vectors. Overexpression of ATM or CHK2 increased the ubiquitination of CABIN1 protein (Figure 4A and B). We also observed that the ubiquitination of CABIN1 in response to UV irradiation was attenuated in the presence of caffeine (Figure 4C). Thus, these results indicate that the DNA-damage kinase-mediated phosphorylation of CABIN1 is prone to ubiquitination upon DNA damage.
CRL4DDB2 ubiquitin ligase participates in degradation of several target proteins upon DNA damage (21–24). We thus postulated that CRL4DDB2 may be involved in CABIN1 degradation upon DNA damage. To address this, the binding of DDB2 to CABIN1 was first tested. Indeed, HA-tagged DDB2 readily co-immunoprecipitated with Myc-tagged CABIN1 and vice versa (Figure 5A and B). To identify the region of CABIN1 protein involvement in interaction with DDB2, Co-IP assays were performed with HA-tagged DDB2 and Myc-tagged CABIN1 deletion mutants. Myc-tagged CABIN1 fragments carrying 701–900 and 901–2220aa (amino acid) regions showed strong binding ability with DDB2 and rather weaker binding affinity in 501–700 aa regions (Figure 5C and D). We next performed binding assays with DDB1 and CUL4A which acted as an E3 ligase complex with DDB2. The results showed a strong interaction with CABIN1 (Figure 5E and F). As an adaptor protein of CUL4 ligase, DDB1 associates with numerous substrate receptors including DDB2 which brings the specific substrates to the E3 ligase complex. To determine whether DDB1 and DDB2 share the binding region of CABIN1, Co-IP assay was carried out using CABIN1 deletion mutants and DDB1. DDB1 was bound to CABIN1 fragments carrying 901–2220 aa regions that partially overlapped with the binding sites of DDB2. In addition, we confirmed that Myc-CABIN1 associated with HA-CUL4A by Co-IP assay (Figure 5G).
To address the possibility that the CRL4DDB2 ubiquitin ligase may be involved in the degradation of CABIN1 under DNA damage condition, we next examined the knockdown effect of DDB1 or DDB2 on the CABIN1 protein level upon UV irradiation. Using the lentivirus-mediated shRNA silencing system, DDB1 and DDB2 were efficiently knocked down in HCT116 cells as evaluated by real-time qPCR and immunoblot analysis (Figure 6A and B). A notable observation was made in the UV-DDB knockdown cells which showed a significant increase in the amount of CABIN1, suggesting that degradation of CABIN1 might be mediated by CRL4DDB2. Furthermore, overexpression of HA-tagged or V5-tagged DDB2 caused reduction of endogenous CABIN1 protein and increased the ubiquitination of CABIN1 (Figure 6C and D). HCT116 cells were infected with scrambled shRNA or DDB2 shRNA lentivirus and CABIN1 stability was subsequently determined using the cycloheximide chase. As expected, knockdown of DDB2 expression delayed CABIN1 protein decay.
We have previously shown that CABIN1 affects p53 target gene expression by co-occupying their promoters in the absence of genotoxic stress. Therefore, the changes in protein levels of CABIN1 influence the mRNA expression of p53 target genes. To test whether UV-DDB could regulate p53 activity through degradation of CABIN1, we assessed the mRNA levels of GADD45 and p21 in sh-DDB-treated cells. Two different combinations of effective shRNAs were used to address off target effects (Supplementary Figure S6). Transient depletion of UV-DDB reduced UV-induced expression of GADD45 and p21 due to the high levels of CABIN1 even after DNA damage (Figure 7A). However, double RNA interference of UV-DDB and CABIN1 restored the mRNA levels to those of controls. We also observed an increased expression of GADD45 in cells expressing exogenous DDB1 and DDB2, which coincided with the reduction of CABIN1. Moreover, the increase of GADD45 expression was not observed in p53-deficient cells (Figure 7B). To investigate the effect of UV-DDB knockdown on promoter co-occupancy, ChIP assays were performed. The results showed that knockdown of UV-DDB blocked CABIN1 dissociation from GADD45 promoter upon genotoxic stress (Figure 7C).
Based on the current results, we propose a working model for CABIN1 degradation upon DNA damage (Figure 8). CABIN1 acts as a repressor of p53 in the absence of genotoxic stress. When DNA damage is inflicted on the whole cells, however, CABIN1 is rapidly phosphorylated, ubiquitinated, and degraded in order to activate the expression of p53 target genes.
We demonstrated that the CABIN1 protein is phosphorylated and ubiquitinated in response to DNA damage and that this post-translational modifications cause the rapid degradation of the CABIN1 protein. In this study, we defined a mechanism of CABIN1 degradation including the factors responsible for these modifications. Phosphorylation of CABIN1 is mediated by ATM and CHK2 kinases and occurs on multiple residues of CABIN1 (Supplementary Figure S3A and B). Depletion of ATM or CHK2 increases CABIN1 protein stability upon DNA damage, and overexpression of either one causes an increase in the ubiquitination of CABIN1.
In addition, the CRL4DDB2 ligase complex was found to target CABIN1 for ubiquitination and degradation. No reports have suggested that UV-DDB recognizes and binds to a specific phosphorylated motif, but it is still possible that phosphorylation of CABIN1 at multiple sites could alter the binding affinity between CABIN1 and the CRL4DDB2 complex. To test this hypothesis, we performed reciprocal co-IP assays of CABIN1 and DDB2 in various conditions. Our results show that UV irradiation increased the binding affinity between them, but pre-treatment of caffeine or CHK2 inhibitor II blocked that effect (Supplementary Figure S5). These results suggest that activities of DNA-damage kinases affect the binding affinity of CABIN1 and DDB2. Moreover, ATM- and CHK2-dependent phosphorylation of MDMX is reported to be important for its degradation and p53 activation which is a similar consequence to CABIN1 phosphorylation (29–31). At present, the precise mechanism of the functional interplay between damage kinases and CRL4DDB2 ubiquitin ligase remains to be elucidated, and further studies are required to determine the exact phosphorylation and ubiquitination sites that are critical for CABIN1 protein degradation.
In fact, we found several phosphorylation sites on CABIN1, but a single residue mutation did not cause a significant difference in CABIN1 protein stability, suggesting that phosphorylation of multiple residues are needed for its degradation. We recently found a mutant construct which seemed to be more stable than wild-type protein under DNA damage; however, it is yet to be tested thoroughly (Supplementary Figure S3C). We also tested whether deletion mutants of CABIN1 are subjected to ubiquitination. Both Myc-tagged CABIN1 fragments carrying 1–900 and 901–2220 aa regions were highly ubiquitinated, implying that ubiquitination also occurs on multiple residues (Supplementary Figure S4).
Our previous studies demonstrated that CABIN1 brings a repressive complex consisting of histone modifying enzymes including HDACs and HMT to the binding partner for repressive regulation (8). It has been shown that CABIN1 restrains p53 activity by regulating chromatin structure on the p53 target promoters. This explains how p53 can occupy the target promoters without activating the gene expression and also suggests a need for their rapid dissociation mechanism after the DNA-damage signal. In this study, we showed that CABIN1 is a target of the CRL4DDB2 ubiquitin ligase complex, thus the protein level of CABIN1 is influenced by overexpression or knockdown of UV-DDB.
To confirm the correlation between CABIN1 and p53 target gene expression, the mRNA level of GADD45 was checked in cells expressing either a high level of UV-DDB or depleted UV-DDB. As expected, the protein level of CABIN1 fluctuated with UV-DDB expression and therefore, the mRNA level of p53 target genes was, in turn, influenced by them.
It is noteworthy that DDB2 is also known as a p53 target gene (32–34). Genotoxic stress causes the transcriptional activation of the p53 target genes including DDB2, which mediates the ubiquitination of CABIN1 and finally boosts p53 activity by eliminating the repressor. We assume that this is a very efficient mechanism for positive regulation of p53 activity upon DNA damage.
Previous studies have also shown that p53 is required for efficient NER of UV-induced DNA lesions (35). It has been proposed that p53 acts not only as a transcriptional activator of DDB2 and XPC, the global genome repair (GGR)-specific damage recognition genes, but also as a chromatin accessibility factor required for chromatin relaxation in order to make DNA lesions accessible for NER (36,37). In the context of the relationship between p53 and CABIN1, there is a high possibility that CABIN1 may influence the NER process.
In this work, we demonstrated a novel mechanism of CABIN1 degradation mediated by the cooperation of the damage kinases and the CRL4DDB2 ubiquitin ligase. Our findings broaden the functional understanding of CABIN1 in gene repression and reveal a possible interplay between damage kinases and the CRL4DDB2 complex.
Supplementary Data are available at NAR Online: Supplementary Figures 1–6.
The National Creative Research Laboratory Program [2012R1A3A2048767 to H.-D.Y.]; Mid-Career Research Program, National Research Foundation of Korea [NRF-2007-0056786 and NRF-2010-0007646 to H.-D.Y.]; World Class University Program of the MEST and the NRF [R31-2008-000-10103-0]. Funding for open access charge: the National Creative Research Laboratory Program [2012R1A3A2048767].
Conflict of interest statement. None declared.
The authors thank Dr Chin Ha Chung for His-tagged ubiquitin plasmid.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's Canada Institute for Scientific and Technical Information in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Bad night for QPR manager Mark Hughes following defeat to Reading as Chelsea draw Manchester United
Wednesday 26 September 2012
Pavel Pogrebnyak's late backheel saw Reading come out on top 3-2 last night in an enthralling third-round Capital One Cup tie at Queen's Park Rangers, in which they twice had to come from behind.
QPR got off to a great start when Junior Hoilett scored a superb solo effort 14 minutes in, although Kaspars Gorkss levelled things up against his former club minutes later. Rangers regained the lead when Djibril Cissé fired home from distance, but yet again the visitors responded with a 25-yard Nicky Shorey free kick, before Pogrebnyak backheeled home nine minutes from time. The former Fulham striker then missed a late penalty and Mark Hughes, the QPR manager, also saw Alejandro Faurlin hobble off injured. Hughes said: "To lose it in that manner is disappointing but it is understandable to a certain extent given the changes and the injuries we have at the back."
Alexander Tettey fired Norwich City into the last 16 with a 1-0 win over Doncaster Rovers at Carrow Road, where there was a minute's silence before kick-off in memory of former manager John Bond, who has died aged 79.
Chris Hughton, the Norwich manager, said: "There was a nice respect from the crowd."
In Scotland, Rangers showed they can still compete with the top sides in the country by dumping SPL leaders Motherwell out of the Scottish League Cup.
Motherwell's hopes of claiming a first win at Ibrox since 1997 were dashed by second-half goals from Lee McCulloch and Dean Shiels as Rangers booked their place in the quarter-finals.
Sunderland v Middlesbrough
Swindon v Aston Villa
Wigan v Bradford
Leeds v Southampton
Norwich v Tottenham
Liverpool v Swansea
Chelsea v Manchester United
Reading v Arsenal
Ties to take place Tuesday 30/Wednesday 31 October
Poor should stop blaming inequality on the rich – says Warren Buffett (the world's third richest man)
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Apr 19, 2012
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Apr 19, 2012
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"Particle Fever" inspires some excitement of its own
The premise to "Particle Fever," Milwaukee Film's members screening selection last week, doesn't exactly scream spectacular entertainment. A film about scientists questing through a world of classrooms and computer screens for an invisible theoretical particle and debating harder-than-diamond scientific theory sounds less destined for the big screen and more doomed for high school classrooms, playing to drowsy students on a pre-break half day.
Yet Mark Levinson's documentary about the discovery of the Higgs boson in 2012 and the experiment's five-year buildup defies its educational film expectations. Yes, side effects likely include a rudimentary understanding of the current state of particle physics, but it's never simply a dense, dry lecture. Rather, "Particle Fever" weaves together an intellectually and emotionally engaging story that efficiently translates years of scientific anticipation, frustration and excitement – even to viewers whose understanding of stuff like protons extends as far as the Ghostbusters' weapon of choice.
The mission is the Higgs boson, boldly nicknamed "God particle." The experiment's methodology is almost amusingly simplistic: smash two beyond microscopic particles together and see what flies out of the wreckage. However, the machinery behind it – a monumental, ring-shaped particle collider a decade and over 100 nations in the making, built underground near Geneva, Switzerland – is anything but basic.
If discovered, the Higgs boson could point physicists in the direction of answering some of mankind's greatest questions about the universe. If not, those same physicists will have to take a long, sobering look in the mirror, realizing that decades of theories and research have led them barking up the wrong tree. Simplified even more, it serves as a progress check: Either the experiment shows science is on the right track, or it's back to page one. Or the ridiculous third option that it forms a black hole that eats the planet (spoiler alert: It doesn't).
There are some big conceptual ideas at play in "Particle Fever," yet much of the film focuses on the little people making it happen. Levinson's movie gets down in the trenches with the scientists, from the people running the experiments out in Switzerland to a duo of theorists dreaming up ideas from a distance (one particularly horrifying one involving multi-verses could basically make physics null, void and impossible to predict).
Oscar-winning editor Walter Murch manages to take seven years of footage, compress that and find the fascinating story, one that bounds back and forth easily between the experiment and the ideas, naturally blending explaining the dense concepts with simple yet effective graphics with personalities without sacrificing anything in the process. Early on, "Particle Fever" wrangles tension from merely waiting for a bleep on a computer screen.
The result is a story that moves and carries a surprisingly dramatic charge.
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Some people just aren't a fan of selfie-taking European Union ladies, it seems.
Good luck breaking up with the girl who's already made a shrine to you made out of stolen locks of your hair and that t-shirt you've been missing for the last week.
In response to Swift's recent breakup with Harry Styles, Laina rewrote the lyrics to One Direction's "What Makes You Beautiful," showing that even Overly Attached Girlfriend has realized something is up with how Swift goes through boyfriends.


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Commissioner Scott Soares
Commissioner, Department of Agriculture Resources (DAR)
This Memorial Day Weekend, I'm excited to be partaking in one of my favorite New England spring-through-fall pastimes – going to Agricultural State Fairs! I'll be headed to the Sheep and Woolcraft Fair in Cummington this Saturday (at Fairgrounds Road) for the kick-off of what is gearing up to be a great fairs season.
Did you know it was two Merino sheep that launched our Commonwealth's rich and diverse history of fairs? In 1807, Massachusetts native Elkanah Watson decided to exhibit his animals at a public square in Pittsfield. A few years later, agricultural societies were established to sponsor and promote the state's agricultural fairs of which DAR has been a big part.
Today, our agricultural fairs remain a favorite destination point for the more than 3 million residents and visitors each year.
The Commonwealth's agricultural fairs feature highlights such as exhibits, livestock competitions and showings, farm-fresh food, and local crafts. Some fairs also have fireworks displays, tractor or pony rides, pig racing, and pie eating contests. There are also wine and beer making competitions, wood carving or cooking demonstrations, and concerts.
Find a fair near you in our fairs brochure.
Find a fair using our interactive map.
This year the American Automobile Association (AAA) has kindly partnered with us to also feature hard copies of our 2010 Fairs Brochure at their AAA clubs. Or, you can send an email request to receive a hard copy to firstname.lastname@example.com.
From fairs to farms to farmers' markets, our Agri-Google map on our new MassGrown & Fresher website will connect you to all of your favorite agricultural destinations. www.mass.gov/massgrown
Whether you like the big fairs or the small community ones – I hope to see you there!
Recent Posts
2015 DAR Agricultural Calendar: April posted on May 14
A lamb at the Cummings School of Veterinary Medicine at Tufts University in North Grafton. Photo by David Cawston April's contest winner was David Cawston who photographed a spring lamb at the Cummings School of Veterinary Medicine at Tufts University in North Grafton. The Cummings School of …Continue Reading 2015 DAR Agricultural Calendar: April
2015 DAR Agricultural Calendar: March posted on Apr 23
Girard's Sugarhouse in Heath, MA. The sugarhouse was built in 1887 and produces around 250-300 gallons of syrup annually. Photo by Michael Girard March's contest winner was Michael Girard who photographed his family's sugarhouse in Heath. Michael Girard has been a sugarmaker since 1961 when he …Continue Reading 2015 DAR Agricultural Calendar: March
2015 DAR Agricultural Calendar: February posted on Feb 25
February's contest winner was Amanda Bettle, who photographed sheep at The Natural Resources Trust of Easton. This photo features Dog, a former 4-H show animal and sole male sheep among the nine ewes in the Natural Resources Trust of Easton (NRT) flock. It is the mission …Continue Reading 2015 DAR Agricultural Calendar: February


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May 13
Today! Sunday, May 13th (Mothers' Day night) The Talking Stick 1411 Lincoln Blvd. Venice, CA 90291 310-450-6052 http://www.thetalkingstick.net/ Harriet's band will consist of Joe Lamanno, Kelly De Sarla, Jennifer Richardson and Andrea Ross-Greene. The guest artists will knock you out and the food is fantastic! The guest artists are Harriet's songwriting students, each of whom will be performing 2 songs and more if there is time. They are: Amanda Abizaid Andrea Ross-Greene Anna Montgomery Art Podell Bill Berry Duff Watrous Gary Stockdale Gene Lippman Kelly De Sarla Michelle Malone Paul Hefti Suzanne Whang
Jan 22
Out with the old year...in with the new shows! Our artists this month know how to put a fresh spin on what they do -- when they're done you'll wish they would start up all over again! (SEE BLOG for artist bios)
Oct 8
Bio: Singer-songwriter Andrea Ross-Greene moved to Los Angeles from Baltimore, Maryland to pursue her career in music. She plays guitar and keyboard. She loves to entertain audiences with her well-crafted songs, soulful voice and wry sense of humor. Andrea writes songs that are meant to move people, w...See Full Bio
Posted a new song: "Thank You For Your Service" http://www.reverbnation.com/c./poni/149596142 #music
"When you have a voice like this, showboating is both unnecessary and rather tawdry. Though comparisons are of limited use, I'll go ahead – I hear elements of the late Phoebe Snow, and – unexpectedly – the creamy qualities of the Peterson sisters (from The Bangles). I have no reservations about associating Andrea with songwriters and singers of that calibre. Now I say, with absolute honesty and not a trace of hyperbole, that Andrea's album is a discreet, delightfully un-flashy masterpiece. I'm enjoying it considerably more than several recent major label offerings by some of my favourite singer/songwriters. Andrea's music has taken beautiful form in the studio and it's hard to imagine how a voice like hers could be captured badly. But without good songs, that wouldn't amount to a great album. Andrea has not only written good songs, she's written brilliant ones, ones that bring to mind the best efforts of Beth Nielsen Chapman, Brenda Russell and Andrea's own mento"


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Jan Thompson writes inspirational international thrillers and contemporary Christian fiction when she is not chronicling regional history or journaling life lessons. She is also an avid reader and enjoys a good book on a pleasant day.
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Tag Archives: Dancing With The Stars
Who Won Dancing With the Stars 2015
Congratulations to Rumer Willis and Val Chmerkovskiy winners of "Dancing With the Stars."
After 10 weeks of grueling practice, competition and a little bit of drama… celebrity dancer Rumer Willis and her professional dance partner and coach Val Chmerkovskiy was were declared the winner of season 20 of ABC's "Dancing With the Stars."
Hines Ward Samba
The scores for the fusion challenge, the dance assignment the competitors received after Monday's show and performed on the finale, were added to the scores they received from that show, where Willis and Lynch had been tied for first place with Galloway in second.
Michael Sam Will Tackle the 'DWTS' Ballroom Hardwood
"Dancing With the Stars" season 20—the 10th anniversary of the show's premiere—will debut on Monday, March 16 on ABC.
The cast announcement for season 20 of Dancing With The Stars is tomorrow!
Hope Solo No Stranger to Controversy
Hope Solo, 32, the star goalkeeper for the Seattle Reign and a two-time Olympic gold medalist from Richland, Washington was arrested and taken into custody on Domestic Violence Charges over the weekend.
She was held without bail for investigation on two counts of domestic-violence assault at the South Correctional Entity Regional Jail in Des Moines, Wash.
Solo was accused of hitting her sister and 17-year-old nephew during a party at her sisters residence in Kirkland, a suburb of Seattle. Solo appeared in court today and entered a not guilty plea to the charges.
Solo is no stranger to controversy. Her outspoken, opinionated demeanor has been the topic of conversation throughout her professional career.
Hope Solo has a net worth of $2.5 Million.
Hope Solo Controversy:
- In a 2012 Hope Solo was interview on CNN. During the interview,on the Piers Morgan Show she spoke about and defended her "public image." Solo spoke about her public image and said "I have a bad rap," she said. "People look at me as selfish, outspoken. But I know who I am." She added, "I don't expect any of the media to be positive. But I know that I'm doing wonderful things for the sport of soccer and I know I'm doing amazing things for female athletes. So I can take it."
- That same year, Solo sent out a harsh tweet criticizing Olympic soccer commentator Brandi Chastain for her negative commentary during the 2012 London games. After the 2007 FIFA Women's World Cup, Solo openly criticized U.S. women's soccer coach Greg Ryan.
- In 2012 U.S. Anti-Doping Agency publicly warned Solo after she tested positive for a banned diuretic — a substance she claimed she didn't know was in premenstrual medication prescribed by her physician. The agency accepted Solo's explanation and she competed in the 2012 Olympics.
Jennifer Lopez and Maksim Chmerkovskiy Dating
Jennifer Lopez isn't wasting any time at all moving on after her split with her twenty-seven-year-old-boy-toy Casper Smart .Lopez, 44, began dating the not-so-smart Smart in 2011, when he was a 24-year-old backup dancer.
Lopez, is reportedly dating "Dancing With the Stars" hottest Maksim Chmerkovskiy.
J.Lo reportedly ended her relationship with Smart. The split came after two transgender models claimed to have had sexting relationships with him.
Two weeks ago, transgender bikini model Sofie Vissa claimed to TheDirty.com that Smart — still dating la Lopez at the time — had asked her for X-rated pics over Instagram and began a private chat with her.
Another transgender model, Xristina Marie, claimed she'd been on a date with Smart in Miami and also had an online relationship with him.
According to sources, the two met when they were working on a performance for the AMA's last year. There was immediate chemistry and the attraction between the two was obvious. Lopez was still with Casper and Maks was dating model Kate Upton. He reportedly broke up with her just a few weeks later.
Maksim Chmerkovskiy
Lopez and Maks have reamined in contact and if reports are true…they are now an item.
More about Maksim Chmerkovskiy:
Maksim Chmerkovskiy first appeared in the 2nd season of the ABC series Dancing with the Stars partnered with actress Tia Carrere.
- Tia Carrere is an American actress, model, voice artist, and singer who obtained her first big break as a regular on the daytime soap opera General Hospital. See photo here
Maksim Chmerkovskiy and Tia Carrere finished in sixth place
Maksim "Maks" Aleksandrovich Chmerkovskiy is a Ukrainian Latin Ballroom dance champion, choreographer, and instructor.
Maksim Chmerkovskiy made it to the final round five times, with two runner-up and two third place finishes.
On May 20, 2014 Maksim Chmerkovskiy won his first mirror ball trophy with celebrity partner Meryl Davis.
Maksim Chmerkovskiy owns four dance studios in greater New York City, including Rising Stars Dance Academy (RSDA)
Meryl Davis and Maksim Chmerkovskiy DWTS Almost Kiss
For those of you who didn't tune in last night to was "Dancing With the Stars," it was a night of spectacular dancing, hot chemistry and the kiss the almost happened."
Maksim Chmerkovskiy and Meryl Davis HOT, HOT, HOT!
Professional dancer Maksim Chmerkovskiy and his Olympic figure skater partner Meryl Davis have radiated chemistry throughout season 18 of the ABC hit reality dance competition and last night we saw the chemistry get even hotter last night as the dynamic dance pair performed their Week 10 Freestyle dance.
Maskim Chmerkovskiy
The sexual tension and attraction between the two has been obviously displayed in all their performances, their rehearsals and their behind the scenes footage. There have even been rumors that the two are having a mirror ball romance outside the dance studio as well….They have been entertaining to watch week after week and last night was no different. Fans were on edge as they share a very up close and intimate embrace on last nights show….which ended with a kiss that didn't happen….
In case you missed it…Hines Ward Samba
Watch video and see for yourself!
Meryl Elizabeth Davis (born January 1, 1987) is an American ice dancer. With partner Charlie White, she is the 2014 Olympic Champion, a two-time (2011, 2013) World champion, the 2010 Olympic silver medalist, two-time (2010, 2012) World silver medalist, five-time Grand Prix Final champion (2009–2013), three-time Four Continents champion (2009, 2011, 2013) and six-time U.S. national champion (2009–2014). At the 2014 Winter Olympics, they also won a bronze medal in the team event.


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May 20, 2015
WASHINGTON [MENL] -- The U.S. Navy demonstration of its new laser weaponin the Gulf appears to mark an effort to impress Congress.
May 16, 2015
WASHINGTON [MENL] -- Iran continues to attack foreign cargo vessels in theGulf.
WASHINGTON [MENL] -- The United States ended a summit with the GulfCooperation Council without a word on the Iranian threat.
May 12, 2015
TEL AVIV [MENL] -- Israel, in the first such procurement in nearly 20 years,has ordered four subsidized naval corvettes from Germany.


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Watch: Phoenix Play Stripped-Down Version of "Entertainment" in Homemade Performance Video
By Jenn Pelly on April 4, 2013 at 11:35 a.m. EDT
Phoenix are gearing up to release their record Bankrupt! this month and headline a number of huge summer festivals in support of it. Today, they've shared a green-screened practice clip of them performing the album's single "Entertainment", which finds the band doing a stripped down version of the song. Also, a beautiful dog wanders in.
Watch: Phoenix Perform Three New Songs Live
By Evan Minsker on March 30, 2013 at 9:45 a.m. EDT
Photo by Kathryn Yu
Phoenix have returned to the stage, so naturally, video has surfaced online of the band performing some new songs from Bankrupt!. Watch their performances of "Bourgeois" (via Consequence of Sound), "Drakkar Noir", and "Chloroform" in Vancouver.
Watch: Phoenix Chat With Pitchfork.tv About New Album Bankrupt! in New Interview
By Pitchfork on March 27, 2013 at 11:31 a.m. EDT
Today, Pitchfork.tv presents a conversation with Phoenix's Thomas Mars and Laurent Brancowitz. Watch as they discuss the making of their album Bankrupt!, including how they implemented harpsichord and why the exclamation point in their album title became essential for them. "Once [the title] had the exclamation point, we fell in love with it," Mars said.
Watch Phoenix Perform "Entertainment" at Studio Q
By Carrie Battan on March 26, 2013 at 3:40 p.m. EDT
Phoenix recently dropped by the studios of Canadian Broadcast Channel's QTV, where they performed a pared-down version of their new single "Entertainment". Thomas Mars looks especially downtrodden. Cheer up, Thomas!
Hear Blood Orange's Version of Phoenix's "Entertainment"
By Carrie Battan on March 26, 2013 at 11:45 a.m. EDT
Phoenix: "Entertainment" (Blood Orange remix) (via SoundCloud)
Producer du jour Dev Hynes of Blood Orange has posted his own take on Phoenix's new single "Entertainment". It's not a remix so much as a cover, complete with female vocalists-- members of UK trio Mutya Keisha Siobhan (the founding members of the Sugababes)-- and Hynes' trademark swaying and swooning 80s touch. Listen above.
Listen: Dinosaur Jr. Cover Phoenix's "Entertainment"
By Jenn Pelly on March 19, 2013 at 9:53 a.m. EDT
Phoenix: "Entertainment" (Dinosaur Jr. Remix) (via SoundCloud)
Dinosaur Jr. have recreated Phoenix's single "Entertainment" off Bankrupt! (out April 23 via Glassnote), as SPIN reports. It's a downtempo, emotional, and slightly unexpected "remix" that sounds more like a cover. Phoenix frontman Thomas Mars told SPIN he saw Dino Jr. in Paris in 1991 and has been a fan since.
Phoenix's New Album Bankrupt Will Feature 71 Bonus Tracks on Its Deluxe Edition
By Corban Goble on March 13, 2013 at 5:10 p.m. EDT
The new album from French rockers Phoenix, Bankrupt!, is out April 23 in the U.S. and April 22 in the UK via Loyaute/Glassnote. Today, they announced they'll be releasing an extensive deluxe edition of the record that will feature a full hour of extras. In total, 71 "demos & sketches" (!) will be included in the package, which is now available to pre-order on iTunes. The album itself is only 41 minutes long.
Watch Phoenix's Bloody "Entertainment" Video
By Jenn Pelly and Evan Minsker on March 7, 2013 at 12:16 p.m. EST
The first single from Phoenix's Bankrupt!, out April 23 in the U.S. and April 22 in the UK via Loyaute/Glassnote, just got a music video. The clip for "Entertainment", directed by Patrick Daughters, is a seemingly fun-loving clip that travels from a forest to the club and ends with some blood.
Listen/Watch: New Phoenix Song "Entertainment"
By Evan Minsker on February 18, 2013 at 2:35 p.m. EST
Here it is, finally: new music from Phoenix. The band have shared "Entertainment", the first taste from their new album Bankrupt! (out April 23 in the U.S. and April 22 in the UK via Loyaute/Glassnote). That's the single artwork up there. The track was premiered on Zane Lowe's show on BBC Radio 1. Watch the lyric video below.
Phoenix Detail New Album, Bankrupt!
By Jenn Pelly on February 12, 2013 at 10:21 a.m. EST
Details have been leaking out since December, but now Phoenix have finally shared the full scope of their upcoming record, Bankrupt!, the follow-up to 2009's breakthrough Wolfgang Amadeus Phoenix. It's out April 23 in the U.S. and April 22 in the UK via Loyaute/Glassnote.
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Eddie Redmayne Might Star in Harry Potter Spinoff
And play a guy named Newt Scamander.READ MORE »
And play a guy named Newt Scamander.READ MORE »
No one knows who wrote this infamously bad, now-legendary Harry Potter fanfiction.READ MORE »
Including Laura Miller on the tense tug-of-war between artists and their creations' biggest admirers, the video-game obsessives who bootlegged Nintendo, and Harry Styles, literary muse.READ MORE »
So many feels.READ MORE »
Sort of the characters, sort of the actors.READ MORE »
He was 79.READ MORE »
Her latest Pottermore update gives a look at Draco's character.READ MORE »
No. 50 is a box of dog biscuits.READ MORE »
Accio Torah.READ MORE »
Scratch your Potter itch before tomorrow's new release.READ MORE »
It will focus on Draco Malfoy.READ MORE »
Think "final," as in Harry Potter and the Deathly Hallows, says Chris Martin.READ MORE »
They're all on Pottermore.READ MORE »
Including what his Horcrux would be.READ MORE »
"I rather enjoyed playing Walder — rather more than is healthy, really."READ MORE »
"Drake-O Malfoy."READ MORE »
David Yates is back!READ MORE »
The little-known Celestina Warbeck.READ MORE »
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Harry is now almost 34 and has some gray hairs.READ MORE »
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Meet Zhao Tao, the Chinese Actress Who Might Beat Cate Blanchett and Marion Cotillard at Cannes
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Samsung Galaxy Note 3 Neo specs and photos surface
This week gave way to both hardware specifications and a leaked photo for a Samsung Galaxy Note 3 Neo. Once rumored as a Samsung Galaxy Note 3 "Lite", it's the stripped down version of the popular big-screen experience.
Given the unconfirmed specs, it appears the Note 3 Neo is actually more akin to the Note 2. Nevertheless, it's still a respectable device and should find a decent following when it arrives. The Samsung Galaxy Note 3 Neo reportedly features a 5.55-inch 720p Super AMOLED display, an 8-megapixel camera, and a 3100 mAh battery.
Samsung GALAXY Note 3 Neo (SM-N750/5) pic.twitter.com/jF7UP1QhsT
— SamMobile (@SamMobiles) January 10, 2014
Powering the smartphone is a 1.7GHz dual + 1.3GHz quad-core (Hexa) processor, 2GB RAM and 16GB internal storage. It will be interesting to see how this first of its kind processor performs as compared to Qualcomm's Snapdragon series. According to SamMobile, it should fall somewhere in the area of a Snapdragon 600 CPU.
There's no indication yet as to which markets are going to carry the Galaxy Note 3 Neo; it's possible it won't come anywhere near the United States.
Mobile World Congress takes place at the end of February where we should look for an Unpacked event or press announcement out of Samsung.
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