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Rick Wilking is a Reuters contract photojournalist based in Denver, Colorado who has been shooting for Reuters for almost 25 years based in Europe, Washington, D.C. and now in Colorado. Rick recently developed the idea of spending time documenting the lives of a Christian "Quiverfull" family who have 15 children due to their belief that all family planning is best left in the hands of God. Rick produced the following piece of multimedia video from his time spent with the Jeub family in Colorado and tells us about the experience below. - Jim Bourg
from Photographers' Blog:
Life with a "Quiverfull" Family – the story behind the story
from AxisMundi Jerusalem:
Collective Punishment in Religious Jerusalem Neighborhoods?
Much ink has been spilled about the riots of Haredi (ultra-Orthodox) Jews in Jerusalem over the past several weeks (See our article on that here). Among some sources, there's a note of disdain for this sector of Jewish population, seen as being contemptuous of the state of Israel while making up the largest portion of the country's welfare recipients.
Tips on reconciling Muslim practises with German schools
The German government and representatives of the country's large Muslim community said on Thursday they had agreed a number of practical proposals to resolve conflicts between German schools and Muslim practises.
Religion crowded out in "cloud" of Ayatollah Khamenei's sermon
Iran's Supreme Leader Ayatollah Ali Khamenei delivered a major address today on the election there. It was in the form of a khutbah, an Islamic Friday sermon that is often the platform for the most important public pronouncements in the Islamic Republic. So one might assume it would be couched in Islamic terminology and religious themes.
French high schoolers struggle with philosophical puzzles
One of the things that makes France so French is the annual philosophy exam that traditionally kicks off the week-long series of tests for the baccalauréat diploma at the end of the lycée (senior high school). While France is a proudly secular state, the questions asked often pose puzzles with ethical aspects that many religions also contemplate. They are usually very broad — some would say impossibly broad — questions, leaving the student to decide how to understand and discuss them in a long essay.
World religious leaders hold their own G8 summit
(Photo: L'Aquila's Santa Maria of Collemaggio Basilica, 13 April 2009/Daniele La Monaca)
They came, they prayed, they appealed.
New on-line forum seeks "common ground" on abortion
A new on-line forum launched on Tuesday seeks to spark discussion among faith and secular leaders and activists about ways to find some elusive common ground on the divisive issue of abortion.
Paris court to rule if Scientology should be shut down in France
How far does the principle of religious freedom go? How much can be accepted in the name of respect for a faith? A Paris court is debating these questions in a fraud case against the Church of Scientology. If the public prosecutor wins the case, Scientology will be convicted of extorting hundreds of thousands of euros from followers on personality tests, vitamin cures, "auditing" sessions and counselling with an "e-metre." It will be disbanded and could also face heavy fines. The French arm of the U.S.-based Scientology denies the charges and says the case violates its freedom of religion.
Visiting the Samaritans on their holy West Bank mountain
(Click on the photo above for a slideshow on the Samaritans)
Samaritan High Priest Abdel Moin Sadaqa was relaxing on his porch watching Al-Jazeera on a wide-screen TV when we dropped by his home to talk about his ancient religion. "I like to keep up with the news," the 83-year-old head of one of the world's oldest and smallest religions explained as he turned down the volume. Told we wanted to make him part of the news, more precisely part of a feature on Samaritanism, he sat up, carefully put on his red priestly turban and proceeded to chat away in the fluent English he learned as a boy under the British mandate for Palestine. Our interview with him and other Samaritans were the basis for my feature "Samaritans use modern means to keep ancient faith."
Will Obama address the Muslim world or the Arab world?
When President Barack Obama delivers his long-awaited speech in Cairo on Thursday, will he address the Muslim world or the Arab world? In the pre-speech build-up, it's being called a speech "to the Muslim world" or "to the world's 1.x billion Muslims" (the estimated total mentioned in different articles fluctuates between 1and 1.5 billion). But the venue he's chosen — Cairo — and all the focus on the Israeli-Palestinian conflict make it sound like a speech to and about the Middle East.


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Tom's Still Got That Need For Speed
Tom's Still Got That Need For Speed
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Tom's Still Got That Need For Speed
Before rubbing elbows with fellow A-listers at Madonna's Oscar party on Sunday, Tom Cruise spent Saturday catching up with his son Connor. The boys took their sunglasses to the racetrack to catch the NASCAR Sprint Cup Series Auto Club 500, and looks like Tom also showed off his football skills. The ladies of the Cruise family decided to sit this one out, but it sounds like a perfect day for some father-son bonding and VIP treatment.
Latest Celebrity & News

subset: commoncrawl


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Jamaica Beaches
The Best Beaches in Jamaica
The Best Beaches in Jamaica
Jamaica is home to over 200 miles of golden sandy beaches and has become one of the world's ...
Beautiful Beaches in Jamaica
Beautiful Beaches in Jamaica
The island of Jamaica is one of the most popular vacation destinations in the Caribbean. With miles ...
Inclusive Beaches in Jamaica
Inclusive Beaches in Jamaica
The Caribbean nation of Jamaica features hundreds of miles of coastline, with beaches all around ...
Beaches at Runaway Bay, Jamaica
Beaches at Runaway Bay, Jamaica
Runaway Bay, on Jamaica's northern coast, has a colorful history replete with pirates, rumrunners, ...
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Beaches Near Jamaica Plain, Massachusetts
Jamaica Plain is inland, southwest of Boston, Massachusetts. Called "J.P." by locals, ...
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text:
November 23, 2014
Stay up-to-date with KAKE News:
HUTCHINSON, Kan-- Police are looking for a man who robbed a north Hutchinson business early Saturday morning.
Around 1:40 a.m. Saturday, Hutchinson Police officers responded to a call that a Kwik Shop on 2617 N. Main had just been the victims of armed robbery.
Police describe the suspect as a man, short in stature, around 5'9 and 130-140lbs wearing a ski mask, camo face mask and dark colored clothing. The man pulled out a silver and black colored handgun and demanded cash from the register.
An unknown amount of cash was taken and the suspect fled on foot in a NW direction carrying a dark colored bag.
Video surveillance is being reviewed and the case is under investigation.
Police ask that anyone with information please call Crime Stoppers of Reno County or Hutchinson PD at 620-694-2834.
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Certain sections of our website are accessible to IoDSA members only.Because of these features, you must be signed in before accessing these closed sections. IoDSA members please sign in, if you are not a member yet, please consider joining now - we'd love you to be part of our community.
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2014/11/17The power is in our hands let's use it
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2014/11/28Being a Director Part 4_28 November 2014
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Cornbread and Bread Pudding
Cornbread and Bread Pudding
Daym takes over the kitchen at Willie Mae's Scotch House in New Orleans.
Brats Brothers
Menage A Trois Sandwich
Neptune's Net
Swan Oyster Depot
Acme Oyster House
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Buckle up and take a tour of the most thrilling amusement parks in America.
3-Ingredient Apps for Thanksgiving
Try Food.com's quick and easy apps for a stress free turkey day.
Food Network's Top Turkey Day Tips
Plan Your Menu with easy, how-to videos from Bobby, Sunny and more.


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Healthy Halloween Treats
Not a Trick: 13 Healthy Halloween Treats
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Not a Trick: 13 Healthy Halloween Treats
The thought of the highs and lows a child gets from binging on Halloween candy is enough to put the scare in any parent. But kids don't need to succumb to sugar's roller coaster ride. Here, we've rounded up 13 healthier Halloween goodies that are sure to be a treat.
Latest Moms


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Inside Preparation Work:
- Read the Handbook of Nature Study pages 821-822 (Lesson 225). Try to share the story of these three constellations with your children either before you go out to view the night sky or if you have a really good memory, while you are looking for the constellations.
- Here is a link to simple explanations and diagrams for the three constellations in this challenge: Well Known Stars and Constellations.
- Autumn Constellations—scroll down to the "facing NE" chart and you will see what Cassiopeia and Cepheus will look like in your autumn sky.
- Advanced Study: Print this chart out and see how many of the constellations you can see in the autumn sky: Autumn Star Chart.
Outdoor Hour Time:
- Take your outdoor challenge time this week during the evening hours. The aim is to get outside and view the night sky, hoping to see the stars if weather permits.
- Use the information, charts, and links both in the Handbook of Nature Study and in the links in the section above to find the Polestar, Cassiopeia's Chair, Cepheus, and the Dragon. Even if you have trouble finding the exact constellations, this activity can be enjoyable for the whole family. Remember to dress warmly or you won't last too long outdoors!
Follow-Up Activities:
- The Handbook of Nature Study suggests in the lesson to draw Cassiopeia's Chair (#1), Cepheus (#5), and the Dragon (#7). These can be done using the chart on page 821 of the Handbook of Nature Study if needed. There is a notebook page included in the ebook for you to use for your sketches if you would like. There is also a checklist on this notebook page you can use as part of the follow-up.
- Advanced Study: Challenge yourself to learn the story of these three constellations as related in the Handbook of Nature Study.
- Advanced Study: Sketch these stars from memory: Polestar, Big Dipper, Little Dipper, Cassiopeia's Chair, Cepheus, and the Dragon. There is a notebook page in the ebook you might like to use as part of this activity.
Join us for this series of challenges every week here on the Handbook of Nature Study. If you want to purchase the Autumn Nature Study Continues ebook so you can follow along with all the notebooking pages, coloring pages, and subject images, you can join the Ultimate or Journey Membership Levels. See the Join Us page for complete information. Also, you can view the Autumn Nature Study Continues content list on the announcement page.
You can also submit any Outdoor Hour Challenge blog entry from October to the next edition of the Outdoor Hour Challenge Blog Carnival by sending the link directly to me by 11/29/14. email@example.com


text:
For more than 100 years, National Geographic explorers have defined the spirit of adventure. Now, provocative interviews and spectacular footage introduce you to a new generation of explorers who take you on an unforgettable journey, that give you a glimpse of where adventure will take us in the future.
Follow Dr. Robert Ballard into the depths of the Mediterranean Sea as he searches for Roman shipwrecks. Join Ian Baker's quest for the mythical waterfall hidden deep within Tibet's Tsangpo Gorges. Dive into vast untouched reaches with deep sea explorer Sylvia Earle. And discover ancient Egyptian secrets with noted archeologist, Dr. Zahi Hawass. Take a bold, exciting look into the future of exploration in Beyond 2000: The Explorers.
Not available for shipment outside of the U.S. and Canada.
National Geographic DVD Exclusives
For the first time ever, National Geographic presents its programming in DVD-R format. Available exclusively through this site, you can now own more of our award-winning specials and documentaries in a high-quality DVD format.
How our DVDs and DVD-Rs Differ
Customers who purchase programs in the NG DVD Exclusives Library will receive a non-interactive, play only disc. There is no chaptering information on these DVDs, but there are chapter marks approximately every five minutes, which make it easy to fast forward through a program. The DVD-R format provides the digital video and audio quality you expect from all of our DVDs.
NOTE: The NG DVD Exclusives titles are not closed-captioned. We apologize for any inconvenience this limitation may cause.
Limited Offer: Spend $100, get $20 off your next purchase!
Make a product purchase of $100 or more from the National Geographic Online Store from 11/3/14 to 11/26/14 at 11:59 p.m. PT, and receive an e-mailed promotion code the following day for $20 that may be redeemed in the National Geographic Online Store. Shipping and handling, taxes, and prior purchases do not apply toward the minimum $100 purchase requirement.
To redeem your promotion code, spend $20.01 or more in merchandise and enter the unique promotion code at checkout in the National Geographic Online Store. Limit one per customer per order. $20 cannot be applied toward digital products; magazines; Genographic kits; donations; gift boxes; shipping and handling; taxes; Weekend Photo Workshops; or CafePress, Barton Cotton, or NG Art Store merchandise. Promotion code may not be combined with certain other offers or discounts; not valid on previous orders and not redeemable for cash. Applies to online orders only. Entire $20 must be used one time in a single transaction; any remaining balance will be forfeited. After promotion code has been applied to order and the order has been submitted, the promotion code is no longer valid. $20-off promotion code is valid through 12/31/14 at 11:59 p.m. PT. Prices not valid in the National Geographic UK Online Store or in the Road Less Traveled Store. Offer is nontransferable and may be modified or canceled at any time. Promotion code is void if items are returned and refund for returned items lowers total order value to less than $100. Offer cannot be resold or bartered.


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One-Hop Shop for Student Services Opens in the Campus CenterAugust 20th, 2014
Several key offices have moved into one location to provide students fast and easily accessible services. The SUNY Canton Registrar's office, Financial Aid office and representatives from the College Association have relocated to the bottom floor of the Richard W. Miller Campus Center creating the "One-Hop Shop." Students can register or change classes, pay their bills, set up a meal plan and get their college identification card all in one location. Previously these departments were in at least two buildings and were across campus from one-another.


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See your red on these designs right now!
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Nice Touch
Two of a Kind (Playing Cards) Envelope Seals Ordered Vegas themed Save the Dates, Invitations, and will order thank you cards.
Signature of Love Envelope Seals Just need a little extra color for the favor boxes to make the wife to be happy. Got 'er done. They got bent during shipping a bit, though, so a more stout shipping container would have been good. Would recommend MagnetStreet to friends.
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Nautical Dreams Envelope Seals We went with ivory linen invitations and then used these on the envelopes. Best way to add a personalized touch and splash of color! Colors, font, spacing was exactly how they were when we edited them. We are extremely happy with them.
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Picture quality is excellent
Pure Rose Envelope Seals I purchased these seals for my Thank You cards. They are extremely clear. I am very pleased with them.


text: I eat lunch here with a friend at least once a month. We've thought about going other places, but like the menu and can depend on good food and service. Plus, the atmosphere is quiet and lovely. I have never had a bad meal here but must admit that I choose the crispy fish tacos almost every time. Occasionally, they... More


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Stuck in a Box for 31 DaysStuck in a Box for 31 Days
Day One: Sealing
Hachigen Ushoda walked slowly toward a small shop in the distance. He squinted at the blurry words on the sign on top of the shop to check that he was in the right area. As he got closer, the bold words became clear: Urahara Shouten, they read. Hachigen smiled slightly. He was indeed in the right place. He started to walk faster at that notion; if he didn't do this now, Soifon-taicho would surely beat him to a pulp.
It was after the Winter War, and he still hadn't granted his promise to Soifon-taicho. "Promise me once more that tomorrow you'll trap Kisuke Urahara in one of your barriers for a month," she had said. It was already two months after the war was over. Everyone needed time to recover from injuries, and it took quite a while before he could get his arm back to normal. He would thank Unohana-taicho later. As of now, Soifon-taicho's expectations were much more important. Frankly, he was rather excited for this moment.
Stopping for a m
Doggone CrazyDoggone Crazy
10/17/09 9:49 p.m. Five dogs have been barking nonstop while a large, loud party is going on.
The blaring music from the stereo had caused a ruckus, inside and outside of the usually peaceful house. Today was a special day; it happened to be a teenager's birthday, and she had invited all her friends for a sleepover and a wild dance party. The usual stuff for birthdays. The girl didn't have a care in the world, and loved to party. As for her friends, they were the same.
All night, the stereo blared, and neighborhood windows shook from the sound. The light from the teenager's room shone brightly, making the dancing teenagers black silhouettes on a square pane of glass. It was 9:30, and the birthday's girls parents were trying to sleep after a long, restless day. However, they never really got the chance to.
Neither did the neighborhood dogs.
The chorus of barking started with the teenager's bloodhound. The bloodhound was a dog that had suffered years of neglecting from the
|Sta.sh Submit


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Home » News » Public Safety
New signs catch eyes of drivers
One of the safety signs that comply to Federal reflectivity regulations and use a national standard for signage on Center Road in Lyndeborough was recently installed. (KATHLEEN BAGLIO HUMPHREYS)
"The sign is not for the people who have been driving that road for 50 years, it's for the new driver who has never been to this area before to alert them to dangers," DOT Highway Design Project Manager Ron Grandmaison said.
Grandmaison said the DOT made its selection after reviewing state-maintained rural roads in its database.
Milford Department of Public Works Director Rick Riendeau said the improved signage meets the Manual for Uniform Traffic Control Devises (MUTCD) guidelines and reflectivity standards.
The bright yellow reflective signs increase visibility, improve advance warning on curves by the use of arrows called chevrons.
He said the new reflectivity standards increase the signs' visibility and may mean fewer signs will be needed.
"We are trying to get the info out to be consistent across the state and also across the country for a national standard," Grandmaison said.
"I noticed a difference on vehicle speeds on Center Road because they put the sharp curve signs and reduced the speeds to 25 mph on curves so it gets people to slow down when they know it's a curve, especially at night," Deware said. "I've noticed myself even slow down because there is that visual stimulus there."
- Rymes Oil employees pull Bradford man from flaming wreck, but driver succumbs to injuries - 0
- Keene man killed in work accident in Troy Monday - 0
- Teen driver dies after being pulled from icy waters in Londonderry - 8
- Trio escapes Claremont fire after smoke wakes resident - 0
- Man suffers life-threatening injuries in Little Cohas Brook crash - 0
- Tractor-trailer pulls down poles, wires, closing part of Route 28 in Epsom - 0
- Commission honors seven for contributions to state firefighter training - 0
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Person found dead in Manchester alleyREADER COMMENTS: 0
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- Dave Solomon's Power Plays: Who knew what and when in PSNH scrubber case? - 0
- Story of Marine Memorial at Hampton Beach begins with Bill Downs - 0
- Immigrants include ethnic dishes at their Thanksgiving table - 0
- Puritans in America: Daniel Webster on religion's role - 0
- Mitt Romney is out front, but says he's not running again - 0
Dave D'Onofrio's Patriots Notebook: Pats put selves in strong positionREADER COMMENTS: 0
On being American: A point for reflection
Your Turn, NH -- Mike Moffett: Republicans can win in 2016 with Kasich at the top of the ticket
Person found dead in Manchester alley
Your Turn, NH -- Mike Moffett: Republicans can win in 2016 with Kasich at the top of the ticket
On being American: A point for reflection


text: Restaurant / Bar / Hotel Experience? Immediate Openings Leicester | - £14k-£30k | From Alpha Gamma Solutions Restaurant / Bar / Hotel Experience? Immediate Openings Perfect if you're looking to take the next step in your professional customer services related career Have fun (really!) while you are... at work Represent UK top brands We are looking for people with a background in restaurants, bars, hotels, pubs, clubs or similar for Customer Service, Sales and Promotions roles. We believe that ... for attitude above all else, the following would really help your application: Some form of previous experience in restaurants, bars, hotels or similar is preferred The ability to make friends ... training and work for top performers. This job was originally posted as www.totaljobs.com/JobSeeking/Restaurant--Bar--Hotel-Experience-Immediate-Openings_job61052492 ... Upload CV Go To Job Save Job


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New Jersey > Ridgefield Park Yellow Pages > Health and Wellness > Health Care Equipment and Supplies > Medical Equipment and Supplies: First Aid Supplies Ridgefield Park
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November 20, 2014 Laboratory Informatics Weekly Update
Volume 12, Issue 43
Welcome to LIMSfinder, the most comprehensive online, interactive magazine devoted to Laboratory Information Management Systems (LIMS) and other Laboratory Informatics Solutions. LIMSfinder is simply the best resource for Information Technology in the Laboratory.
The Key to Interfacing Lab Instruments to LIMS or ELN
Innovate or die!: Genomic data and the electronic health record (EHR)
11/12/2014 -
Bid Opportunity - Laboratory Information Management System LIMS for Newborn Screening
10/23/2014 -
Request for Proposal - Development of a Cloud Based Laboratory Data Exchange Portal
11/21/2014 -
Autoscribe Ltd. PublishesTwo New LIMS Case Studies
11/13/2014 -
The SmartLab Exchange Releases the Periodic Table of Laboratory Information Management
11/12/2014 -
IBM Watson Group Invests in Pathway Genomics to Help Personalize Consumer Health
11/12/2014 -
ARUP Laboratories and PierianDx Create Partnership to Produce More Efficient and Effective Diagnostic Genetic Tests
Introducing the 2014 Edition of the Complete Guide to LIMS & Laboratory Informatics
Introducing the 2014 Edition of:
The Complete Guide to LIMS & Laboratory Informatics
The Laboratory Informatics Institute is pleased to announce the 2014 release of the new, Complete Guide to LIMS and Laboratory Informatics. This reference guide is unique in the LIMS industry by providing a side-by-side comparison of the most popular LIMS products on the market along with complete pricing information and now, we have provided links to demonstrations of the various listed products. Also, we have updated the guide to be completely available on-line and kept up-to-date daily as new information becomes available along with critical information you need to help you document your LIMS requirements.
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Senate proposal would require a certification program to teach the acquisition workforce about small business set-aside programs and more.
Three senators have introduced a bill to establish a training program to help contracting officers understand federal contract set-aside programs.
The Small Business Training in Federal Contracting Certification Act (S. 3444) introduced May 27 would require the Office of Federal Procurement Policy (OFPP) to establish a certification program for the acquisition workforce.
The program would teach those employees about small business set-aside programs that offer special competitions among specific types of small companies, such as service-disabled, veteran-owned small businesses or companies owned by women.
Obama moves on small-business contracting
Senators push for acquisition workforce training
Army applies boot camp approach to acquisition workforce
The program also would train contracting officers on how to determine the company size standards for a procurement and choose the appropriate the North American Industry Classification System (NAICS) codes. Those codes are the government's standard that agencies use to catalog businesses in various industries. There have been allegations in recent years of "code shopping," which refers to choosing NAICS codes that allow favored or larger companies to bid as small businesses, rather than those that most fairly apply to the contract's scope of work.
The legislation would have the Federal Acquisition Institute carry out the program.
Senators sponsoring the bill say it would improve the federal government's small-business contracting efforts.
"Our legislation would help the government to meet or exceed its 23 percent statutory contracting goal for small businesses, which it has yet to achieve," Sen. Olympia Snowe (R-Maine), ranking member of the Small Business and Entrepreneurship Committee, said in a statement. Sens. Mary Landrieu, (D-La.), the committee's chairwoman, and Ben Cardin (D-Md.), a committee member, co-sponsored the bill.
About the Author
Matthew Weigelt is a freelance journalist who writes about acquisition and procurement.
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Nominations are now open for the 2015 Federal 100 awards. Get the details and submit your picks!
Make a nomination today
Steve Kelman considers the costs of corruption in Mexico, China, and most developing countries -- and argues that Americans should keep their own government troubles in perspective.
Agency leaders must proactively invest time, energy and resources to shape the future rather than reactively wait for change to happen.
Meet 24 men and women who are driving key changes in federal IT -- and shaping up as the community's likely leaders of tomorrow.
GSA's administrator talks with FCW about short-term hiring, long-term planning and Robert Frost.
Without a future vision and an appetite for risk, reforms lead down a rabbit hole. There is a better way.
VA's top tech official says he is balancing risks while trying to keep the department's online services open for business.
After five years on the job, the founding director of the Office of Government Information Services believes that a deeper understanding and acceptance of FOIA is seeping into the federal government.
The September attack, made public Nov. 10, potentially puts customer and employee personal information at risk, including addresses, Social Security numbers and emails.
The General Services Administration's Matthew Goodrich predicts more agency-driven authorizations and previews a new two-year road map.
The Small Business Training in Federal Contracting Certification Act (S. 3444) is a big step in the right direction for the small business set-aside programs!
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Mexicorn Dip
Stop Eating, Sour Cream, Families Meeting, Cups Shredded, Onions Slices, Mexicorn Dips, Shredded Cheddar, Cowboy Caviar, Green Onions
Previous pinner wrote, "My husband talked about this for two days - 4 thawed chicken breasts, 6oz. package of Stove Top stuffing mix, 1/2 cup sour cream, 1 can of cream of chicken soup. Chicken on bottom - Stove Top over chicken, Cream of Chicken/Sour Cream mix on top. Cover and cook for 4 hours on low."
My husband talked about this for two days - 4 thawed chicken breasts, 6oz. package of Stove Top stuffing mix, 1/2 cup sour cream, 1 can of cream of chicken soup. Chicken on bottom - Stove Top over chicken, Cream of Chicken/Sour Cream mix on top. Cover and cook for 4 hours on low.
Sour Cream, Stuffed Mixed, Chicken Breasts, Crock Pots, 1 2 Cups, Tops Stuffed, Stoves Tops, Cups Sour, Chicken Soup
The Pursuit of Happiness: EASY Crockpot Chicken & Stuffing
Our favorite crockpot dinner!! 4 ingredients. Delicious Chicken and Stuffing dinner.
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Step by step instructions on turning spaghetti squash into noodles! Top with your favorite pasta toppings for a low-carb, paleo alternative.
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Cecily Goes to Chef School: Make the Best Grilled Fish Ever
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Garlic Parmesan Salmon Foil Pack. Very simple and healthy dinner with only 420 Calories and 5 Carbs. #diet #lowcal #lowcarb #healthy


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Vince McMahon Laying Low At TV Events, WWE Merchandise Sales, Papa Shango
by Amish Patel - November 28, 2012
- At WWE's weekly Raw events, Vince McMahon generally only meets with producers and other officials, with most talent only encountering him when they head to the curtain. Furthermore, he does not attend approximately half of the SmackDown tapings because he feels it necessary that Paul "Triple H" Levesque gain experience in taking charge of shows because he will ultimately be the leader of the company.
When Levesque is in charge of events, the atmosphere is described as being more chaotic. It is said that when disagreements or problems arise, there is nobody in the Vince role of putting his foot down and saying, "This is how it's going to be done."
– WWE merchandise sales has been described as "haves" and "have nots." A few talents are reportedly making "great money," with all others not faring too well.
– During a recent interview, Charles Wright revealed that WWE had plans for him to return as grittier version of Papa Shango in 1997, but the idea was ultimately shelved due the introduction of another dark, supernatural character in Kane. In response to feedback on Twitter, Wright said Jerry Lawler assisted him with the character's look and that he may post online a Polaroid photo that was taken then if he can find it.
(source: Wrestling Observer Newslettter)
|CM Punk Interview: Says He's Done With WWE, Speaks About Why He Left, Talks With Vince & HHH, Lots More
The Hottest WWE news, photos and videos delivered to your inbox in one convenient newsletter. Sign up now and give it a try!
|WWE Survivor Series Report - 11/23/14
|CM Punk Interview: Says He's Done With WWE, Speaks About Why He Left, Talks With Vince & HHH, Lots More
|WWE RAW Report - 11/24/14
|Sting Makes His WWE TV Debut, Possible Match Against Triple H?, Update on The Authority
|Backstage News on Sting vs. Triple H or The Undertaker, Speculation on Sting's WWE Future


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page 2 - Maranello's Man
Michael Schumacher - though not loved by all - is the best driver of his generation, and one of the fastest of all time. The grid will not be the same without him.
By January 18, 2007 / Photos by Paul-Henri Cahier
"I think his major contribution is that he turned Ferrari around from 21 years of failure," says Stewart. "Drivers come and go. Lots of people say, 'Is the sport going to survive without Michael Schumacher?' When Jim Clark died, everyone said, 'Will the sport survive this?' And it was the same with Ayrton Senna.
"The sport is bigger than any single driver, and I don't think it will actually miss Michael. With the number of starts, the batting average is not that extraordinary. But it is extraordinary that the latter part of his career was so successful, because he drove Ferrari. It wasn't Luca di Montezemolo, it wasn't Jean Todt.
"Michael did it, he rejuvenated that team and drove it. They knew he could win motor races, and he was driven to please them. He put it together better than anybody else I've known. I chose not to drive for Ferrari, because I thought that was a task that I didn't want to take on at that stage of my career."
Stewart is of course right to say that Grand Prix racing will carry on regardless, but many insiders are concerned about what might happen to the wider public interest in the sport. Michael reached out to people who were not previously F1 junkies, and not just in Germany or Italy. It's perhaps not a coincidence that for 2007, ringmaster Ecclestone has scratched the second races in both those countries.
Even Michael's brother Ralf, who has tired of answering questions about his older sibling during his own decade in F1, acknowledges that we are stepping into the unknown. "It's a strange feeling thinking that he's gone now," says Ralf. "We've spent 10 years together here. What is it changing? I don't know. The German media, spectators and sponsors are worrying a bit, and we have to wait and see how other German drivers, including me, can fill this role or not.
"To me it's very simple. As soon as somebody is quick and wins the championship, it will not matter. If we're not, and we saw that already in 2005 when Michael didn't win, the interest slows down. It's the same in every market, as soon as there is no driver competing for the championship."
Schumacher was a supremely talented driver, and a great ambassador for the sport. Despite his extraordinary wealth, he remains a fundamentally decent and unspoiled human being. His perfect holiday involves horses, Harleys and unspoiled countryside, preferably in the U.S. Midwest.
However, it's impossible to gloss over the darker side of his nature. In Monaco last year a clumsy piece of gamesmanship saw him stop on the track and ruin the final qualifying laps of his furious rivals. It was an astonishing piece of behavior, and immediately revived memories of infamous incidents earlier in his career.
Twice in title showdowns he instigated controversial collisions with rivals Damon Hill in Adelaide '94 and Jacques Ville-neuve at Jerez '97 that left even his supporters struggling to defend him. And there were many other occasions when his forceful style left another driver with nowhere to go. He even leaned on brother Ralf a few times.
Former Ferrari driver Ivan Capelli makes the interesting observation that Michael's great strength was his ability to solve as many problems as possible before he even got in the car. When something came up unexpectedly in the white heat of a racing situation, and he had to react on instinct, it did not always compute.
There's no doubt that his uncompromising, hard-ass nature made him the driver he was. Winning was everything.
"It is a controversial area, we can't deny it," says former Ferrari technical director Ross Brawn, Michael's staunchest supporter. "But it's part of his DNA, and that's the way it is. He can't stand for anyone else to win. It hurts him for someone else to win a race. I like that in a guy. The opposite is someone who doesn't care, and you certainly don't want that. Sometimes it borders on the edge, we all know that, but that's what makes a World Champion. I'd be disappointed if he was the other way.
"I think he's set the standard that other F1 drivers have to achieve. I think you can see some of the other drivers realizing what needs to be done, the commitment you need, the level that you can take it to. I think he's just raised the professionalism of F1 drivers to a new level."
This year Michael will have no choice but to stand by and watch others win, and quite possibly, win in his beloved Ferrari. However, he has given his full blessing to his successor, Kimi Räikkönen. The pair have always got on well socially, and the uncomplicated, stone-faced Finn has never done or said anything that has in any way been disrespectful to Schumacher. The same cannot be said for a lot of the drivers he raced with over the years. He's also happy to hand over the reins of the Prancing Horse to Felipe Massa, his last teammate and a man who did not make his final year difficult. Michael's own future remains unclear. Ferrari has left the door open for him to have some sort of ambassadorial or advisory role, and it remains to be seen how that will pan out. Just don't expect him to be seen at too many Grands Prix in the short term, there's not much point in retiring to spend time with the family if you keep up the traveling. His name alone will earn him millions per annum in endorsements for the rest of his life, but at some point he is sure to want to do more than just smile for another shampoo, oil or watch photo shoot. That energy will have to be channeled somewhere.
Burned out after his years with Michael, Brawn will himself be away from the F1 scene this year, although he may yet return to a managerial role at Ferrari. Like everyone else, he wonders what the sport will be like without the man he worked with for a decade and a half.
"It's difficult to judge until we get there. But he had been a huge character, a huge person in F1, almost since he arrived. Some young guys are coming through, but whether they will ever make as big an impression as he made, I don't know. I think he'll certainly go down in history as one of the greats of F1, and I think we'll all be sorry to lose him. F1 will remain, it will find its new heroes, and it will evolve. But there will not be another driver like Michael Schumacher."


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It looks like post-UFC life is treating Tito Ortiz just fine. Ortiz banked a hefty $300,000 purse for his split decision victory over fellow UFC Hall of Famer Stephan Bonnar in the headlining bout of Saturday's Bellator 131 event, according to information released to Sherdog.com by the California State Athletic Commission. Bonnar took home $100,000 in the losing effort, although he could have doubled that amount had he defeated Ortiz. Aside from the two former UFC stalwarts, lightweight champion read news >>
Not surprisingly, Tito Ortiz topped the Bellator 131 fighter salaries with a reported payout of $300,000. read news >>
Guess what haters, the joke is on you! Bellator 131 headliner Tito Ortiz banked a whopping $300,000 payday for disposing of Ultimate Fighting Championship (UFC) import Stephan Bonnar at last Sat. night's (Nov. 15, 2014) Spike TV mixed martial arts (MMA) event in San Diego, California (highlights). "The American Psycho" took home $100,000. That probably doesn't sit well with lightweight champion Will Brooks, who claimed just $62,000 for turning away former division kingpin Michael Chandler in the read news >>
Main-event winner Tito Ortiz was the big earner at Saturday's UFC 180 event and accounted for $300,000 of the $632,600 disclosed payroll.Filed under: Bellator, News read news >>
Tito Ortiz and Stephan Bonnar were the main attractions of Bellator 131, and they also went home with the largest paychecks. read news >>
When Gennady Golovkin enters the ring Saturday to face Marco Antonio Rubio, it now amounts to a non-title catchweight bout. read news >>
Conor McGregor plus three of the four combatants in the main and co-main events at UFC 178 were the highest salaried fighters on the payroll. The UFC 178 salaries are out, and the flyweight champion is your highest earner. Demetrious Johnson netted $183,000 (including a $54,000 win bonus) for his submission win over Chris Cariaso, who pocketed $24,000. Eddie Alvarez earns a $100,000 base pay in his UFC debut, which is higher than his initial $70k/$70k salary the UFC planned to start him with las read news >>
Some fight fans say UFC flyweight champion Demetrious Johnson is one of the most overlooked fighters in the promotion, but his check from UFC 178 says otherwise. Mighty Mouse walked home with a cool $183,000 after his second-round submission victory over Chris Cariaso in UFC 178's headliner Saturday night, as Sherdog reported. Johnson earned $129,000 to show up and was awarded an additional $54,000 for the win. Meanwhile, featherweight contender Conor McGregor wasn't far behind, taking home a he read news >>
Conor McGregor may not have topped the bill at UFC 178: Johnson vs. Cariaso, but he certainly stole the show, and finished second on the disclosed payroll. read news >>
If time is money, then Conor McGregor did pretty well for himself at UFC 178. The Irish featherweight sensation earned a little over $1,415 per second in purse winnings alone in just his fourth UFC fight, cashing in a $150,000 check for his sub two-minute victory over Dustin Poirier on the night's pay-per-view card, according to information released to MMAFighting.com by the Nevada Athletic Commission. Throughout weeks of pre-fight hype, McGregor predicted he would knock out Poirier inside the f read news >>
UFC 178 is in the books and payouts and salaries are in the checkbooks. Here's a complete list of last Saturday night's (Sept. 27, 2014) earnings for all the fighters competing at the "Johnson vs. Cariaso" pay-per-view (PPV) event in Las Vegas, Nevada. UFC 178 fighter payouts for those athletes competing at the "Johnson vs. Cariaso" pay-per-view (PPV) event last weekend (Sat., Sept. 27, 2014) from inside MGM Grand Garden Arena in Las Vegas, were released earlier today by the Nevada State Athleti read news >>


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Got a kid who just won't go to sleep? These proven tips from iVillage moms can help you streamline your bedtime -- fast!
(16 Photos)
Lela Davidson
Sep 1, 2013 at 11:42PM
"Introduce a sound machine from the beginning. The white noise will become something they quickly associate with the sound of sleep. Both of my kids have used them since Day One. The best part is you can take them anywhere so even a hotel has the same comforting sounds that their own bedroom provides for a good nights sleep." -- Kim, Rogers, AR
Yummy recipes, DIY projects, home decor, fashion and more curated by iVillage staffers.
The very dirty truth about fashion internships... DUN DUN @srslytheshow http://t.co/wfewf
Behind-the-scenes pics from iVillage.
Enter your expected due date:


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Shura's 'Touch' May Make You Want to Kiss Someone: VIDEO
"Touch" from London-based, Russian-born singer, producer, remixer and video editor Shura is an ethereal track about "the sadness of not being with somebody anymore, but also the infinite tenderness of your time together" starring the singer's group of friends.
Shura herself makes a cameo in the clip, but doesn't kiss anyone.
Enjoy, AFTER THE JUMP...
Continue reading "Shura's 'Touch' May Make You Want to Kiss Someone: VIDEO" »


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Dr. Fredro C. Knight, MD
Emergency Medicine, Our Lady Of The Angels Hospital
- 433 Plaza St
Bogalusa, LA 70427
- 1 Office Location
- (985) 730-6711
Dr. Fredro C. Knight has been practicing medicine for 16 years. Dr. Knight graduated from the Meharry Medical College School of Medicine in 1998. He practices medicine in Bogalusa, LA and specializes in Emergency Medicine. Dr. Knight is affiliated with Our Lady Of The Angels Hospital.
Gender: Male
Years in Practice: 16 Years
Languages Spoken: English
Medical School: Meharry Medical College School of Medicine
Graduation Date: 1998
Hospital Affiliations: Our Lady Of The Angels Hospital
Specialties: Emergency Medicine


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''"Fantasia"'' is the most ''"adult"'' classic full length animated film Disney ever made. This makes it already a bit different than most of their other full length animated films. Almost all of the segments take place in the dark.
!!! Fantasia (1940)
* ''"Toccata and Fugue"''
** Near the end of the segment a strange shape can be seen walking from the camera back to the distance. It is shaped as a coffin and accompanied by very frightening music.
* ''"The Nutcracker Suite"''
** The start of the segment has an eerie atmosphere to it. Tiny little lights floating in a pitch dark forest. The music doesn't help much either.
** The MoodWhiplash of contrast between the quiet fish dance and the loud Russian flower dance, can be startling.
* ''"The Sorcerer's Apprentice"''
** The wizard ([[WordOfGod Yen]] [[BackwardsName Sid]]) has a very eerie piercing stare in his big eyes. He never ever speaks, which makes him appear all the more menacing.
*** Interestingly the opening scene with him sets up this trope, then subverts it--Yen Sid seems to be summoning some sort of creepy, disturbing skull imagery...only for it to blend together and become a beautiful butterfly.
** The idea of something as inanimate as a broom suddenly walking around ... and then taking a will of its own!
** [[ShadowDiscretionShot Mickey chopping up the broom]]. A DeletedScene (existing in pencil test form) had Mickey doing it ''onscreen''.
** The way the chopped up broom slowly but surely comes back to life. Mickey then hearing sounds from behind the door and taking a peek...
** Mickey almost drowning in the whirlpool.
** Yen Sid providing a JumpScare moment by suddenly hitting Mickey with the broomstick.
* ''"The Rite of Spring"''.
** The creation of the universe is very eerie, due to the threatening music. Especially when Earth comes closer and it seems as if the viewer will be crushed by the planet.
** The never slowing down or stopping lava stream.
** The scenes taking place in the deep ocean. The music itself is very spooky, especially during the first scene where organisms split up to form cells. What makes this scene particularly frightening is that most children have no idea what is going on!
** The Pteranodon [[FamilyUnfriendlyDeath getting eaten]] by a Mosasaur. This happens onscreen and [[DownerEnding ends that part of the segment]]. Worse still, the Pteranodon gets grabbed by the Mosasaur's jaws head first and then slowly gets dragged into the water, [[NothingIsScarier in almost complete silence, no less]]. There's no blood or anything, but it's still pretty shocking.
** The appearance of those Gallimimuses who head towards the river to drink. The music is eerie and they move like zombies, complete with glazy stare and jerky movements.
** The brutal battle between the Stegosaurus and the T-rex.
*** Hell, the T. rex himself! If he doesn't scream {{Prehistoric Monster}}, then I don't know what does!
** The dinosaurs dying from starvation is also very disturbing. Especially the one who wants to dig up something to eat and then slowly realizes there isn't anything there. While he sits there, tired of his fruitless search the desert wind starts blowing against his back and you realize he is doomed.
*** If that's not terrifying enough, there's the T. rex collapsing from the heat. Once it was a powerful creature feared by all, now even it is rendered powerless by nature's wrath and is forced to join in the march towards extinction.
** Or what about the solar eclipse at the end of the segment? It's just so eerie that something so familiar as the sun can be so frightening.
* ''"The Pastoral Symphony"''
** Although not a villainous character, the pitch-black pegasus with the glowing red eyes is a little creepy looking.
* The ''[[http://www.youtube.com/watch?v=V8Ca_edg6RE Night On Bald Mountain]]''. Everything that ever went bump in the night, all rolled up in one burning Black Mass presided over by a [[AttackOfThe50FootWhatever 50-foot]] [[{{Satan}} Chernabog]], complete with GlowingEyesOfDoom, demonic leers of delight, and terrifying snarls. Do NOT watch this after dark with the lights off. It's one of Disney's [[SugarWiki/MomentOfAwesome highest points in animation]], with [[SugarWiki/VisualEffectsOfAwesome stellar special effects]]... ''and they just make it worse''.
** The idea that this truly haunting event happens more often than once a year, near that quiet little city near the bald mountain... You just hope that the villagers don't realize what happens near their town each night!
** Chernabog's eyes! And his powerful gigantic arms are also something you don't wish to encounter in real life.
** Chernabog grabbing a handful of demons while he looks at them with an endeared smile. Then he just drops them into the lake of fire, as if he lost interest...
** The harpy grabbing a demon but then (accidentally?) dropping him, causing him to plummit to his doom inside a seemingly bottomless pit of fire!
** A particularly unsettling moment in that scene would be when Chernabog picks up a bunch of beautiful, fiery succubus-like creatures and watches them dance seductively on his hand...then he places his other hand over them and they all [[BalefulPolymorph suddenly transform into hideous beasts]]. Their expressions afterward imply that [[FateWorseThanDeath the transformation isn't a fun experience for them]].
** Chernabog ends his night of horrors by bringing the mass of spirits, demons and fire together and producing a bright, white light [[http://img3.wikia.nocookie.net/__cb20120712225812/disney/images/6/68/Chernabog_18.PNG while his arms stretch toward the sky]]. Given that Disney was intending this to be a stand-in for the Devil himself, [[FridgeBrilliance and another name for Satan was Lucifer, or "Light Bringer"...]]
!!! ''Fantasia 2000''
* ''The Firebird Suite''.
** The embodiment of life and nature destroyed by a horrific firebird. Which comes in the form of a volcanic eruption.
** One of the most heart-stopping, [[JumpScare jump-scaring]], EyeOpen moments ''ever''. The song already came prepackaged with its very own music sting, and the animators ''ran with it.''
** The scene where the Nature Sprite scrambles up a tree to escape the Firebird. [[HopeSpot It really looks like she might escape...]] and then we see the Firebird [[EnemyRisingBehind slowly rise up from behind her]]...
** Look closely at the smoke that rises from the Firebird as it awakens. It forms the shape of dozens of howling and screaming ''skulls''.
** The Firebird, and especially the skulls, become more horrifying when you find out this section was [[RippedFromTheHeadlines based on]] [[http://en.wikipedia.org/wiki/1980_eruption_of_Mount_St._Helens the 1980 Eruption of Mount St Helens]], a real-life natural disaster ''which killed 57 people.''
* ''Rhapsody in Blue'', when Rachel's parents look out their office windows and [[AdultFear see their daughter running into busy traffic]].


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Originally written on Mets Merized Online | Last updated 11/14/14
At seasons end Dickey called he and Wright a "package deal." Now that the Mets have locked up David Wright to an extension, they will probably shift their attention to the 2012 Cy Young award winner, RA Dickey. Could it be the reason why David Wright agreed to sign on the dotted line was because he and Dickey are truly a package deal, and now the Mets are going to lock up Dickey next? It could be the case-who knows at this point? There has been tons of speculation regarding what the Mets are going to do with regards to Dickey. There have been numerous trade rumors this off-season. Rightly so, as it seems this would be the optimal time to restock the team with young prospects in exchange for Dickey. That's called selling high. Then, in the midst of the rumors, they were somewhat discredited when other MLB team executives said they thought the Mets were using the trade talks to gain an edge when negotiating with Dickey. That last part doesn't really make much sense. Dickey wants to be...
Original Story: http://metsmerizedonline.com/2012/11/...
PLAYERS: RA Dickey, David Wright, Johan Santana
TEAMS: New York Mets
TEAMS: New York Mets
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New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.).
ABSTRACT New primer pairs of genomic DNA microsatellite markers were tested to assess the genetic diversity of eleven date palm genotypes. The results indicated that out of thirty, only seven primers (23.3%) failed to amplify the expected PCR fragments, while thirteen primers (43.3%) amplified monomorphic banding patterns and the remaining ten primers (33.4%) generated polymorphic banding patterns. A total of 77 alleles have been observed with a mean of 7.7 alleles per locus. The average of gene diversity was 0.80 ranging from 0.6 (in marker DP168) to 0.9 (in two markers DP157 and DP175). These new co-dominant markers will be a starting point for researchers making use of the markers for genetic mapping and diversity analysis of date palm.
Article: ANALYSIS OF GENETIC DIVERSITY OF DATE PALM (Phoenix dactylifera L.) CULTIVARS FROM MAURITANIA USING MICROSATELLITE MARKERS[Show abstract] [Hide abstract]
ABSTRACT: In Senegal, as in the most Sahelian countries, date palm is an embryonic stage of development. The existing varieties of date palm produce poor quality dates that fruit maturation coincides with the rainy season which is a favorable period for the development of pathogens. Thus, selection and introduction of cultivars with fruit ripening is done outside of the rainy season has become a priority for the development of date palm in Senegal. This preliminary work aimed at characterizing 5 early Mauritanian date palm cultivars: ("Ahmar", "Medina", "Tijib", "Edaghed", "Tiguidert"), and males ("Emacine") using microsatellite markers. These cultivars are clustered in 92 samples from 5 oases (Aoujeft, Chinguitti, Kseir Torchane, Terjit and Teyeret) in Mauritania and analyzed with 16 SSR markers. Only 14 markers have amplified successfully all samples and are polymorphic. The results reflected a great genetic variation between cultivars and the obtained dendrogram shows an individualization of cultivars in spite of a certain degree of similarity. An excess of heterozygous is observed in the cultivars. Very low genetic variability for "Ahmar" cultivar, moderate for "Medina" cultivar and very high for "Tijib" cultivar has been observed. Three clusters grouping samples of different cultivars were obtained. No genetic specificity was observed for "Emacine".International Journal of Science and Advanced Technology. 02/2012; 2(Volume 2 No 2 February 2012):2221-8386.
Article: Near-infrared electroluminescence emission from an n-InN nanodots/p-Si heterojunction structure[Show abstract] [Hide abstract]
ABSTRACT: An n-InN nanodots/p-Si(1 1 1) heterojunction diode was fabricated by plasma-assisted molecular beam epitaxy. The device shows clear rectifying behaviour with a turn-on voltage of approximately 1.2 V at room temperature. The near-infrared electroluminescence (EL) can be observed under forward bias, which covers a wide wavelength range. In comparison with the photoluminescence spectra, the maximum of the EL spectra has a blueshift which is probably due to the size quantization effect of small-sized InN nanodots and their stronger contribution to the EL intensity. On the other hand, there is an obvious enhancement of the less dominant transitions on the short wavelength side of the EL spectra, which may arise from the recombination of the injected holes with the extremely high-density surface electrons of InN nanodots.Journal of Physics D Applied Physics 05/2012; 45(21):215102. · 2.53 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Molecular typing of 377 female date palm trees belonging to 18 Libyan cultivars and representing common genotypes in the central Libyan oasis of Al Jufrah was performed using 16 highly polymorphic microsatellite or SSR loci. A total of 110 alleles with an average of 6.88 alleles per locus were scored indicating the high level of polymorphism existing among the cultivars thus allowing their genetic fingerprinting. Moreover 28 alleles out of 110 were fixed. All the cultivars were characterized by negative values of the Fixation Index (F) due to an excess of heterozygotes with respect to HW equilibrium. The pattern of genetic diversity among cultivars was estimated by codominant genetic distances and presented by principal coordinates analysis (PCoA). The observed pattern evidences the genetic diversity existing among cultivars that allow distinguishing them easily. The average dissimilarity internal to each cultivar ranged from 0 to 21. Seven cultivars showed value zero indicating no genetic difference within cultivar in agreement with their Fixation Index (F = 1). A varietal identification key was also built using multiloci genotyping with only three microsatellite loci that identified 23 alleles in total. The possibility to attribute the unknown male plant to a cultivar was also considered and male parentage analysis was performed. Fifty-five male plants out of 63 were assigned to a definite cultivar with high confidence level. The positive result obtained in identifying males confirmed the suitability of SSR for clone fingerprinting and cultivar identification, thus opening new prospects for date palm breeding.3 biotech.
Page 1
New microsatellite markers for assessment of genetic diversity
in date palm (Phoenix dactylifera L.)
Khaled Elmeer•Hina Sarwath•Joel Malek•
Michael Baum•Aladdin Hamwieh
Received: 12 March 2011/Accepted: 11 May 2011/Published online: 27 May 2011
? The Author(s) 2011. This article is published with open access at Springerlink.com
ellite markers were tested to assess the genetic diversity of
eleven date palm genotypes. The results indicated that out
of thirty, only seven primers (23.3%) failed to amplify the
expected PCR fragments, while thirteen primers (43.3%)
amplified monomorphic banding patterns and the remain-
ing ten primers (33.4%) generated polymorphic banding
patterns. A total of 77 alleles have been observed with a
mean of 7.7 alleles per locus. The average of gene diversity
was 0.80 ranging from 0.6 (in marker DP168) to 0.9 (in two
markers DP157 and DP175). These new co-dominant
markers will be a starting point for researchers making use
of the markers for genetic mapping and diversity analysis
of date palm.
New primer pairs of genomic DNA microsat-
Simple sequence repeats
Phoenix dactylifera ? Microsatellite marker ?
Date palm (Phoenix dactylifera L.) belonging to the
Arecaceae family, originated in Mesopotamia and thou-
sands of cultivars have been reported (Hanachi et al. 1998).
Date palms is a diploid (2n = 2x = 36), and the predicted
genome size is estimated to be approximately between 550
and 650 Mbp long (Malek 2010). Date palms have always
been clonally propagated to ensure the identity and uni-
formity of the cultivars and the morphological markers of
many traits are often unreliable or extremely difficult to be
estimated correctly, especially because of the influence of
environmental factors (Elhoumaizi et al. 2002). Therefore,
marker technology for DNA fingerprinting has become
increasingly important in recent years to discriminate
among closely related cultivars. Several marker systems
have been used to study the genetic diversity of date palm.
In brief, randomly amplified polymorphic DNA (RAPD)
fingerprints have been used to identify date palm acces-
sions in Algeria (Benkhalifa 1999), in Morocco (Sedra
et al. 1998), in Tunisia (Trifi et al. 2000), in Saudi Arabia
(Al-Khalifah and Askari 2003), and in Egypt (Soliman
et al. 2003; Adawy et al. 2006). Amplified fragment length
polymorphic (AFLP) markers have been applied to study
the genetic diversity of date palm cultivars in Egypt and
California (Cao and Chao 2002; El-Assar et al. 2005;
Adawy et al. 2006).
Microsatellite or simple sequence repeat (SSR) marker
has been used in plant diversity analysis; the popularity of
these markers is due to their ease of amplification by
polymerase chain reaction (PCR), their co-dominant nature
and their typically high levels of allelic diversity at dif-
ferent loci. There are numerous reports suggesting the
usefulness of microsatellite markers for measuring the
genetic variability in a wider taxonomic range (Ryberg
et al. 2002; Li et al. 2007; Kawka et al. 2007; Chan et al.
2008; Banhos et al. 2008).
Microsatellite markers have been developed and used to
investigate genetic diversity in Phoenix dactylifera (Bil-
lotte et al. 2004). They used (GA)n microsatellite-enriched
library to develop 16 microsatellite markers. More recently
K. Elmeer (&) ? H. Sarwath
Biotechnology Centre, Ministry of Environment, Doha, Qatar
e-mail: email@example.com
J. Malek
Weill Cornell Medical College in Qatar, Doha, Qatar
M. Baum ? A. Hamwieh
International Center for Agricultural Research in the Dry Areas
(ICARDA), Aleppo, Syria
3 Biotech (2011) 1:91–97
DOI 10.1007/s13205-011-0010-z
Page 2
further 17 microsatellite loci were developed by con-
structing two microsatellite-enriched libraries of date palm
using (GA)n and (GT)n repeats (Akkak et al. 2009). These
microsatellite markers have been used to assess the genetic
diversity and relationships of date palm varieties in Tunisia
(Zehdi et al. 2004), in Sudan (Elshibli and Korpelainen,
2007), in Oman (Al-Ruqaishi et al. 2008), and in Qatar
(Ahmed and Al-Qaradawi 2009). However, still 33
microsatellite markers are not enough to cover the whole
genome and give a comprehensive assessment of biodi-
versity in date palm. This creates an urgent need to develop
more of microsatellite markers in this genome. The
objective of this research is to use recently developed new
microsatellite markers in date palm genome to assess the
genetic diversity in date palm.
Materials and methods
DNA extraction
Eleven cultivars were chosen randomly from different
locations in Qatar namely Khalas, Shaishi, Barhi, Hilali,
Sukari, Khnaizi, Shahil, Khasab, Rzaiz and Lulu as females
and one male Phahel.
The frozen young leaf tissues of date palm collected
from each of the cultivars were first cleaned carefully with
distilled water to remove the waxy layer and then 1 g of
leaf sample was cut into small pieces and grinded into fine
powder using liquid nitrogen. DNeasy Plant Maxi kit
protocol (QIAGEN) was used to extract DNA by following
the manual instructions of the kit (DNeasy Plant Hand-
book). The obtained DNA were quantified and qualified
using Nanodrop Spectrophotometer. For further estimation
of the DNA quantity 2 ll was loaded on 0.85% agarose gel
at 100 V for 30 min. The gels were stained in ethidium
bromide and visualized under UV light.
Primer design and PCR amplification
The sequences of 30 new primer pairs of genomic DNA of
date palm were obtained from the International Center for
Agricultural Research in the Dry Areas (ICARDA) recently
published by Hamwieh et al. (2010). These primers were
made in (IDT) Integrated DNA Technologies, Inc and
derived from an assembly draft of the date palm genome
generated by whole genome shotgun next generation DNA
sequencing issued by researchers in the Weill Cornell
Medical College in Qatar (WCMC-Q).The length of the
primers, sequence information, repeat motifs, allele size and
melting temperature (Tm) are shown in (Table 1). Poly-
merase chain reaction was performed in a total reaction
mixture of 25 ll containing 2 ll (20–30 ng) of total
genomic DNA, 12.5 ll of AmpliTaq Gold?360 Mastermix
(Applied Biosystems), 1 ll (5 pmol/ll) of primers each and
8.5 ll of nuclease free water. Amplification was carried out
in a Veriti 96 Well Fast Thermal cycler (Applied Biosys-
tems) under the following conditions: initial denaturation
95 ?C for 10 min, 35 cycles (denaturation 95 ?C for 30 s,
annealing temperature depending on primer for 30 s,
extension 72 ?C for 1 min), final extension 72 ?C for 7 min.
In order to achieve better resolution, electrophoresis was
performed using the Spreadex?EL600 Mini gel (Elchrom
Scientific). These gels are manufactured from a novel
monomer and have 39 higher resolving power than any
other synthetic gels with working separation range
40–600 bp and optimal separation range 150–350 bp. The
amplified DNA fragments 2 ll were diluted with 3 ll
distilled water and 2 ll of loading dye making a total
volume of 7 ll were loaded on to the gel. Electrophoresis
was carried out at 150 V for 150 min in 19 TAE buffer
(30 mM). The gel was stained with ethidium bromide
(EtBr) solution (1 mg/ml) for 30 min at 350 rpm and
destained with double distilled water for 45 min at
400 rpm. The DNA banding patterns were visualized on a
UV transilluminator and documented using Gel Docu-
mentation System (Alpha Innotech).
Data analysis. Microsatellite bands were precisely
measured by gel documentation system AlphImager EC by
Alpha View software V.188.8.131.52 and scored for each geno-
type. Each polymorphic DNA band at particular position
on the gel was treated as a separate character and scored as
allele size. Data were then computed with the PowerMar-
ker software V3.0 (Liu and Muse 2005) to detect the per-
centage of heterozygosity and the phylogenetic relationship
among the genotypes on the basis of the allele's size. The
phylogenetic diagram was drawn by TreeView V.1.6.6
(Page 1996).
Results and discussion
Thirty new primer pairs of genomic DNA microsatellite
markers of date palm were tested to assess the genetic
diversity of eleven date palm genotypes. The results
indicate that ten of the thirty markers (33.4%) generate
polymorphic banding patterns at expected size (Fig. 1).
These primers are: DP151, DP157, DP159, DP160, DP168,
DP169, DP170, DP171, DP172 and DP175 (Table 1).
These new co-dominant markers will be a starting point for
researchers making use of the markers for genetic mapping
and diversity analysis of date palm. However, these results
are relatively lower when compared to Akkak et al. (2009),
who selected seventeen markers (41%) as polymorphic,
after screening of forty-one simple sequence repeats. The
results also show that thirteen markers (43.3%) amplified
923 Biotech (2011) 1:91–97
Page 3
Table 1 Forward and reverse primer sequences, repeat motifs and expected sizes of microsatellite loci and its status of amplification of SSR loci
of date palm
Primer name Primer's sequence
Tm(?)Expected sizeMotif repeatStatus of amplification
3 Biotech (2011) 1:91–97 93
Page 4
monomorphic banding patterns (Fig. 1), while the remain-
ing seven primers (23.3%) failed to amplify the expected
PCR fragments.
The microsatellites examined were highly polymorphic
possessing a great number of alleles, a total of 77 alleles
with a mean of 7.7 alleles per locus were scored, however,
the number of alleles varied between 4 in primer DP168
and 12 in primers DP157 and DP175 (Table 2). The
number of alleles per locus detected in this study were
higher than those scored by Ahmed and Al-Qaradawi
(2009) who marked 40 different alleles with a mean of 4
alleles per locus by examining 15 Qatari date palm
The numbers of allele per locus detected in this study
were almost equivalent to those graded by Zehdi et al.
(2004) who recognized 7.14 alleles per locus when exam-
ining 46 Tunisian date palm accessions using 14 micro-
satellite loci.
Elshibli and Korpelainen (2007) identified 21.4 alleles
per locus, which were more than the numbers of alleles per
locus detected in this study; it may be due to using more
number of microsatellite loci (16) in addition to using
different genotype area 68 Sudan and Morocco date palm
The ten primers used in this study successfully produced
clear amplified SSR band sizes ranging from 108 bp
(marker DP160) to 274 bp (marker DP175), similar to
Ahmed and Al-Qaradawi (2009) results which ranged from
100–300 bp.
The sequences flanking microsatellite sites are generally
conserved within species and also often in closely related
species (Gupta and Varshney 2000). Interestingly, the ten
SSR markers tested in this study formed mean of 7.8
genotype numbers (Table 2), however, the highest were
10 different genotypes scored in marker DP175 (206/
262 bp, 198/198 bp, 236/274 bp, 196/196 bp, 200/258 bp,
Fig. 1 Markers amplified monomorphic and polymorphic banding patterns at expected size: M is 50 bp Genplot marker, date palm sample
Cultivar No. 1 Khakas, 2 Shaishi, 3 Barhi, 4 Hilali, 5 Sukari, 6 Khanaizi, 7 Shahil, 8 Khasab, 9 Razaiz, 10 Lulu and 11 Male
Table 1 continued
Primer name Primer's sequence
Tm(?) Expected sizeMotif repeat Status of amplification
-, Non-amplification; ?, amplification of monomorphic band; ??, amplification of polymorphic band of desired PCR product with Qatari date
943 Biotech (2011) 1:91–97
Page 5
198/234 bp, 226/226 bp, 196/236 bp, 226/250 bp and
234/270 bp) represented in Barhi, Hilali, Khalas, Khasab,
Khnaizi, Lulu, Male, Razaiz, Shahil, and Shaishi, respec-
tively while cultivar Sukari share the same genotype with
cultivar Khnaizi.
On the other hand, only 4 different genotypes was
renowned in marker DP186 (166/175 bp with Barhi, Hilali
and Shaishi, 172/172 with Khalas, 163/163 with Khasab,
Khnaizi, Lulu, Male, Shahil and Sukari, 163/172 bp with
Razaiz cultivar.
The mean of gene diversity was 0.80 (Table 2) ranging
from 0.60 (for locus DP168) to high diversity 0.90 (for two
loci DP157 and DP175). This high level of gene diversity is
similar to 0.83 reported in date palms germplasm from
Sudan (Elshibli and Korpelainen 2007). This high level of
diversity is expected because of the unique mechanism
responsible for generating SSR allelic diversity by repli-
cation slippage. Replication slippage is thought to occur
more frequently than single nucleotide mutations and
insertion/deletion events, which generated the polymor-
phisms detected by RAPD analysis (Powell et al. 1996).
The heterozygosity was 0.42%, much lower than 0.84
and 0.82 in Sudan and Morocco date palm cultivars,
respectively (Elshibli and Korpelainen 2007). The average
major allele frequency was 0.32 ranging from 0.13 in
marker DP175 to 0.59 in marker DP168. Similarly, the
polymorphism information content PIC value which is
commonly used in genetics as a measure of polymorphism
for a marker locus used in linkage analysis, ranged between
0.55 in DP168 to 0.89 in DP157 and DP175, with an
average of 0.77 per marker. Allelic variation might be
correlated with the number of repeats within a particular
microsatellite locus. A positive relationship was found
between the number of repeats and the PIC of earlier
reports in tomato (Smulders et al. 1997; Areshchenkova
and Ganal 1999; He et al. 2003, Pritesh et al. 2010). In this
study (Table 2), DP168 with the lower PIC (0.55) has eight
repeats compared to DP157, which has PIC (0.89) with
nineteen repeats, which is in agreement with the finding of
Pritesh et al. (2010). Marker with lower PIC (0.08) has
three repeats compared to marker which has PIC (0.40)
with seven repeats. Moreover, similar to the report of He
et al. (2003) no relationship was found between PIC and
the number of nucleotides per repeat. However, there are
reports that the polymorphism level in trinucleotide repeats
is lower than that in di-nucleotide repeats for rice (Blair
et al. 1999) and ryegrass (Jones et al. 2001).
A dissimilarity matrix between Qatari date palm culti-
vars showed an average dissimilarity coefficient ranging
from 0.00 to 1.00. The cultivars studied here were highly
divergent at the DNA level. The lowest dissimilarity
coefficient value was observed between Khnaizi and
Sukari cultivars (0.25) which seem to be the nearest two
varieties and can be closely regrouped (Fig. 2). Ahmed and
Al-Qaradawi (2009) found that the similarity coefficient
Table 2 Genetic biodiversity
information of Qatari date palm
using 10 SSR markers
Marker Major allele
Genotype no. Allele no. Gene diversity HeterozygosityPIC
Mean 0.327.807.700.80 0.420.77
Fig. 2 Phylogram Tree of Qatari date palm based on Distance of Nei
1983 by NJ method
3 Biotech (2011) 1:91–9795
Page 6
value of Qatari date palm ranged from 0.00 to 0.75 while
Zehdi et al. (2004) found that the similarity coefficient
value of Tunisian date palm ranged from 0.3008 to 0.7885.
The next closet cultivars with 0.43 dissimilarity coefficient
value were obtained between Khasab and Shahil. Dissim-
ilarity coefficient value of 1.00 was obtained between
Barhi and each of Khasab, Khnaizi, Shahil and Sukarii
cultivars, indicating that how far is the relationship
between Barhi cultivar and those cultivars (Fig. 2).
Molecular markers can provide an effective tool for effi-
cient selection of desired agronomic traits because they are
based on the plant genotypes and thus, are independent of
environmental variation. Several molecular markers are
being currently employed, of which simple sequence
repeats (SSRs) or microsatellites are the most widely used
types. SSRs are not only very common but also hyper-
variable for number of repetitive DNA motifs in the gen-
omes of eukaryotes (Hamada et al. 1984; Edwards et al.
1991; Vosman and Arens 1997; Rallo et al. 2000; van der
Schoot et al. 2000). All the other cultivars displayed dif-
ferent levels of dissimilarity but still were grouped with
each others (Fig. 2). It is suggested that the variation or
polymorphism of SSRs are a result of polymerase slippage
during DNA replication or unequal crossing over (Levin-
son and Gutman 1987).
The phylogenetic diagram (Phylogram) illustrates the
divergence between the studied Qatari date palm cultivars
(Fig. 2) and suggests their tree branching. Male (Phahil)
cultivar was clustered separately from the rest of other
cultivars. Mainly the phylogenetic tree showed two major
clusters, the first included four cultivars (Shahil, Khasab,
Sukari and Khnaiz) and the second cluster contained six
cultivars (Khalas, Shaishi, Hilali, Barhi, Razaiz and Lulu);
however, Lulu and Razaiz were grouped in a separate sub
cluster (Fig. 2).
Genetic diversity is desirable for long-term crop
improvement and reduction of vulnerability in plants to
important crop diseases. Measurements of genetic diversity
can be used in breeding programs to increase the genetic
variation in base populations by crossing cultivars with a
high level of genetic distance as well as for the introgres-
sion of exotic germplasm. Molecular genetic diversity
estimates are extremely useful for intellectual property
protection, particularly in the determination of essential
derivation. The genetic diversity estimates based on
molecular marker data may be compared to a minimum
genetic distance which indicates that two cultivars are not
essentially derived (Lefebvre et al. 2001).
Open Access
Creative Commons Attribution License which permits any use, dis-
tribution, and reproduction in any medium, provided the original
author(s) and source are credited.
This article is distributed under the terms of the
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3 Biotech (2011) 1:91–9797


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Obama's TV Picks: Critically Acclaimed Dramas From AMC, HBO, Showtime and Netflix
What the commander in chief is watching (and catching up on).
When it's time for President Obama to unwind with a little TV, he prefers heavy dramas over reality TV.
The president has made it well known that he's a fan of Showtime's Homeland and HBO's The Wire, but he's also said he likes HBO's Game of Thrones and Boardwalk Empire, British series Downton Abbey, which airs on PBS in the U.S., and Netflix's House of Cards, the second season of which he's eagerly awaiting, friends of his told The New York Times.
PHOTOS: 'Breaking Bad's' 10 Most Mind-Blowing Episodes
Obama is also working his way through the DVD box set of AMC's Breaking Bad, the Times reports, adding that even though the series just ended, he's way behind and frequently reminds people not to spoil anything he hasn't seen.
The president also used DVDs to catch up on AMC's Mad Men, according to the Times, telling friends that Elisabeth Moss' Peggy Olson gave him insight into how his strong-willed grandmother dealt with life in a man's world.
What Hollywood Earns
Top Galleries
What's Hot in TV
Hollywood's Notable Deaths of 2014
Watch Johnny Depp's Bizarre, Curse-Filled Speech at Hollywood Film Awards
Watch James Franco and Seth Rogen Meet in the Woods on 'Naked and Afraid'
Bill Cosby: His Career and Family Life in Pictures
Glen A. Larson, Creator of TV's 'Quincy M.E.,' 'Magnum, P.I.' and 'Battlestar Galactica,' Dies at 77


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