text:
Bar Bristol
A place in Santiago where you will find live music and more
Located in central Santiago, Bristol bar offers a variety of spirits, wines, sparkling wines and craft beers which it can be accompanied by exquisite tables to share or sandwiches among others; all under a cosmopolitan atmosphere.
Every week from Wednesday to Friday evenings will be different in your company our live music under jazz and bossa nova style.
At lunchtime you surprised every week with a tasty menu that includes
See what we have here for this week:
Looking for a picture for tea time? In Bristol Bar we offer a wide range of pastries, sandwiches, juices, tea and coffee for those who enjoy this tasty want more time.
You can also find exquisite and enjoy Happy Hours, which includes 2 drinks a choice of over a cutting board.
Make your day is different and full of flavor! To book directly do. cl / restaurant / bar-bristol-hotel-plaza-san-francisco "title =" resrva online "target =" _self "> here or if you prefer you can contact us at 23604454 or contact us @ plazasanfrancisco . cl
Monday to Sunday 08:00 - 24:00
Check out our lunch menu here
Happy Hours
If you enjoy a delicious drink, cutting boards, sandiwch in the best environment in downtown Santiago, you can not miss visiting Bristol Bar, we offer the best service and cuisine.


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|Presentations Stream 1
Acoma A & B
|Presentations Stream 2
Lobo A & B
|Tuesday, April 15th
4.00pm - 6.00pm
6.30pm - 9.00pm
Arrival and Onsite Registration, SUB Building, UNM
Keynote Speech and Introductory Dinner. SUB Ballroom C
Welcome – Ann Cunliffe.
Conference Dinner and Keynote
Changing the "Subject" of Qualitative Research: Communication, Organizing, and the Politics of Common Sense
Dennis Mumby, University of North Carolina, USA.
|Wednesday, April 16th
9.00am – 10.40am
|Chair: Michaela Driver
Women Academics in the Cognispheric Workplace:
A Digital Duoethnography
Janet Salmons, Capella University & Victoria Boynton, State University of New York at Cortland, USA
Avatars, Personas, and Other Tricks of the Trade of Online Ethnography.
Dariusz Jemielniak, Kozminski University, Poland.
"Consultations on Conflict": How Executive Coaches Describe and Rationalize the Dark Side of Female Assertiveness in the Workplace.
Margaret M. Brooks, Arizona State University, USA
Enactivism as an Approach to Social Cognition.
The Example of How Social Cognition of Artificial Intelligence Impacts the Organization of Work.
Aleksandra Przegalinska. Kozminski University, Warsaw, Poland.
|Chair: Sarah Tracy
Going Back to Our (non-linear) Roots: Celtic Mythology and Narrative Methods.
Jackie Ford, University of Leeds, Nancy Harding, University of Bradford & Mariana Fotaki, University of Warwick, UK.
The Silent Concept in Qualitative Research
Daniella Fjellström, Uppsala University, Sweden, & David S. A. Guttormsen, University of Warwick, UK
Their Stories/My Research: A Critical Reflection of Ethnographic Organizational Communication Research.
Andy Kai-chun Chuang, Metropolitan State University of Denver, USA
Shaping Food Production: A Dialectical Analysis of Organizational Tensions about what Constitutes Healthy Foods.
Alison Henderson, University of Waikato, NZ,
Linda Putnam, University of California Santa Barbara, USA.
|10.40am – 11.00am
11.00am – 12.30pm
|Chair: Jackie Ford
Researching the Lacking Subject: Inquiring into Subjectivity from a Lacanian Perspective.
Michaela Driver, Western State Colorado University, USA
Three Voices, Three Subjectivities, Three Struggles.
Liz Hayes, Corp Community; Clare Hopkinson, University of West of England, & Alan Taylor, Coventry University, UK
Capturing and (Re)presenting Subjectivities: Examining Soundscapes of Voice/Silence in Organisational Life.
Diane Burns, University of Sheffield, UK.
Subjectivity in Organizational Identity Inquiry – Case of Auto-ethnography.
Lukasz Sulkowski, Jagiellonian University, Cracow, Poland & University of Social Sciences, Warsaw, Poland.
|Chair: Chris Mclachlan
Critical Realism in Leadership Studies: The Application of the Critical Incident Technique.
Gregory Bott & Dennis Tourish. Royal Holloway, University of London, UK.
Engaging Meaning in Motion: Proposing a Critical Interview Methodology.
Amy K. Way & Robin Kanak, Villanova University &
Sarah J. Tracy, Arizona State University, USA.
Toward Wholeness: An Action Research Strategy to Improving Provider and Client Health.
Carizma Chapman & Kisha Bashikaratee, Loma Linda University, USA
Nonprofit Sector Leadership: What Does it Really Look Like?
Monika Hudson & Keith Hunter, University of San Francisco, Darlene Rodriguez, Salem College, & Pier Rogers, North Park University, USA.
|12.30pm – 1.15pm
1.15pm – 3.00pm
|Chair: Janet Salmons
Police Stories as Alienation: Exploring Our Writing with "Triple Reflectivity".
John T. Luhman & Andy F. Nazario, Eastern New Mexico State University, USA
(Inter)subjectivity and the Research Pair.
Carrie M Duncan & Sara R.S.T.A. Elias, University of Missouri, USA.
Multiple Lenses of Self and Other: A Dialogical Reading of Video Recordings, Others' Accounts & One's Own Fieldnotes to Explore "What is Going on Here" in Social Interaction.
Clare Mumford, Open University, UK.
Investigating Institutional Work through the Lens of Lived Experiences of Organisational Change.
Louise Grisoni, Oxford Brookes University, UK.
|Chair: Karen Locke
Relational Subjectivity: Recovering our Living Relations to our Surroundings.
John Shotter, University of Leeds, UK
Working on Organizational Contradictions through Narratives: A Situated Dialogic Action-Research Case.
Giuseppe Scaratti, Elisa Liberati, Laura Galuppo & Mara Gorli, Catholic University of Milan, Italy.
Toward a Meaningful, Multi-sited, Big-data Qualitative Data Analysis.
Sarah J.Tracy, Shawna Malvini Redden, Margaret Brooks, Louise Clark, Phil Mulvey, Michael Shafer, Arizona State University & Elizabeth Hall, University of California, LA, USA.
Hosts, Guests and Textualized Selves: Subjectivity in (Privatized) Sharing Economy.
Karolina Mikolajewska, Kominki University, Poland
|3.00pm – 3.30pm
3.30pm – 5.00pm
|Chair: Karen Golden-Biddle
Subjectivity and the I/We in Team Based Ethnography.
Rebecca Gill & Joshua Barbour, Texas A & M University, USA
A Museum, Shared Professional Spaces & Social Distance: Or Can there be not Auto-ethnographic Ethnography?
Małgorzata Adamczyk, Kozminski University, Poland
The Politics of Access in Fieldwork: Infiltration, Backstage Dramas and Deflection.
Rafael Alcadipani, FGV-EAESP, Brazil & Ann L Cunliffe, University of Leeds, UK.
Ethnographic Practice: An Ontological Odyssey?
Tim Butcher, RMIT University, Australia
Chair: Dariusz Jemielniak
Ontological Angst: Why Critical Realism won't do for Justifying a Mixed Methodology PhD.
Julie Wilson, University of Leeds, UK
Social Epistemology, Epistemic Imbalances and Decision Making Processes in Groups.
Catherine Kelly, University of Roehampton, UK.
Economization of Emotions at the Workplace – How Personal and Social Relations at Work become Objectified and Managed.
Agata Dembek, Kozminski University, University of Warsaw, Poland.
Chair: Andy Kai-chun Chuang
Emancipating Reflexive Voice in Developing Contemporary Graduate Identity: The Role of Synthesis, Critical Thinking and Design Thinking.
Elizabeth Johnston & Anthony Kortens, University of Phoenix, USA
Reflexivity and Disclosure: Methodological Trends in Tension?
James Michael Fortney, Western Washington University & Kate Lockwood Harris, University of Colorado Boulder, USA.
Beyond the Wounds: The SCAR Project as Space for Female Breast Cancer Patients' New Gender Identities.
Hyunji Doh & Donnalyn Pompper
Temple University
|6.30pm – 9.30pm
|Conference Dinner and Keynote Speech. Sheraton Hotel
Self-Making and the Brain
Emily Martin, Professor of Anthropology
New York University, USA
April 17th
9.00am – 10.30am
|Chair: Ann Cunliffe
Expanding Queer Reflexivity: Shifting Social Identities, Shifting Meta-theoretical Commitments.
James McDonald, University of Texas at San Antonio, USA.
Philosophy as Praxis? Navigating Ontology and Subjectivity in Qualitative Interviewing.
Chris Mclachlan & Reece Garcia, University of Leeds, UK
Complicating the Researcher as Privileged Ally in Scholarship on Difference, Power, and Organizing.
Elizabeth K Eger, University of Colorado, Boulder.
|Chair: Donnalyn Pompper
Merging Research, Education and Local Development – Semi-focused Groups for an Engaged Scholarship.
Frederic Bill, Kozminski University / Linnaeus University, Sweden
Banking on the Poor?: Universalizing Discourses of Social Initiatives under the Guise of 'Value'.
Carolyn Day, University of South Florida, USA
Participants as Collaborators: Engaging the Coordinated Management of Meaning (CMM) to guide an Appreciative Cooperative Inquiry.
Ilene C. Wasserman, Innovative Communities at the Workplace
|10.30am – 11.00am
11.00am – 1.00pm
|Chair: Elena P. Antonacopoulou
Subjectivity Dead and Reborn: Managers' Narrative Work to Maintain Agency in Paradoxical Contexts.
Klaus Majgaard, Copenhagen Business School, Denmark.
Miscarriage and Negative Space: Using Autoethnography to Research Taboo Topics.
Elizabeth Siler, Worcester State University & Emily T. Porschitz, Keene State College, USA.
Interrogating Social Identity, Power, and Difference
In Researcher-Researched Relationship Dynamics.
Donnalyn Pompper, Temple University, USA.
Communication in Space, Freedom in Time. Spatialization, Subjectivity, and Representation of Temporal Experience.
Ray Montagno, Ball State University & Ioan Muntean,
University of Notre Dame, USA.
|Chair: Gregory Bott
The Influence of French National Culture upon an American Workplace.
Debra Borkovich & Robert Skovira, Robert Morris University, USA.
Assimilation Barriers, Lost Status, and Family Destabilizations: Perspectives of African Refugees on Organizational Challenges in the US.
Jonathan Camp, Abilene Christian University & J. Garpue Lieway, William V.S. Tubman University, USA
Ethnic Identity as Discursive Practices.
Geetha Karunanayake, University of Hull, UK
A Crystalized Approach to Identity Performances in Airport Security: Connecting Multi-level Discursive Analysis with Embodied, Embedded Ethnographic Research.
Shawna Malvini Redden, Arizona State University, USA.
|1.00pm – 1.45pm
1.45pm – 3.30pm
|Chair: Julie Wilson
The Role of Organization Employment Equity Policies in Promoting Gender Equity within a Transformational Context: A Case of Two South African Universities.
Yvonne Senne, Tshwane University of Technology, Zambia.
Greed, grit, and qualitative research: Stories of securing samples.
Amanda Peticca-Harris & Nadia deGama. York University, Canada, & Sara R.S.T.A. Elias, University of Missouri, USA
Experience, Language and the Subjective/Objective Problem: A Theory for Agile Ethnographic Inquiry.
Robert Joseph Skovira, Robert Morris University, USA
|Chair: James McDonald
Hypothesizing Socio-cultural Sensitivities of Front-line Employees – A Case for Interpretive Phenomenological Analysis.
Lynn Beckles, University of the West Indies, Barbados
The 'Referees' are Biased, Further Solidifying Entrenched Professional Boundaries: An Expository Dilemma of Interprofessional Work in Healthcare.
Selorm Mensah Yevu, University of Hull, UK
A Reflexive Autoethnographic Analysis of Life Lived: Subjectivity Making a Difference to Managing.
Elena P. Antonacopoulou, University of Liverpool, UK & Gelson Silva Junquilho, Federal University of Espirito Santo, Brazil.
The Re-turn to Reflexivity: The Role of Logos and Phronesis in Learning and Changing.
Elena P. Antonacopoulou, University of Liverpool, UK.
|3.30pm – 4.00pm
|Coffee and Depart
|Coffee and Depart

subset: commoncrawl


text:
This Cast-Iron Steel Burner is made of high quality materials and made to last several years. This cooking burner is designed to fit many different models and will meet or exceed OEM specifications.
Fits Sams Gas Grill Models: 5002D, 9701D, 9803S, 9905TB, 9912T, Bakers and Chefs 9701D, Bakers and Chefs GQ-5001D, Bakers and Chefs GQ-5002D, Bakers and Chefs Y0655, Y0655, Bakers and Chefs Y0656, Y0656
Measures: 15.75" X 2.875"
Includes: 6 Cast Iron Burners
Aftermarket, NOT made by Sams
Replacement Part Number: 23301, P2007D, P2007T
All prices are in USD Copyright 2014 Marbeck. Sitemap | Shopping Cart Software by Bigcommerce


text:
Allianz General Insurance Company (M) Berhad
Allianz Malaysia takes pride in being part of the notable Allianz Group which was founded in 1890 in Germany. With approximately 142,000 employees worldwide, the Allianz Group serves more than 78 million customers in about 70 countries.
Allianz officially stepped foot in Malaysia in 2001 when it became the controlling shareholder of Allianz General Insurance Malaysia Berhad ("AGIM"). In 2007, the general insurance business of AGIM was transferred to its wholly-owned subsidiary, AGIC. Following the completion of the transfer of general insurance business, AGIM changed its name to AMB and became an investment holding company, with a general insurance (AGIC) and life insurance (ALIM) subsidiary.
AGIC is one the leading general insurer in Malaysia and has a broad spectrum of services in personal lines; small to medium enterprise business and large industrial risks. AGIC also leverages on the banc assurance agreement with CIMB Bank to reach out to the bank's over-a-million-customer base. The GWP for general insurance business for financial year 2011 reached a mark of RM 1.46 billion.
Allianz Malaysia aims to distinguish itself as the most trusted provider of financial services to suit the lifetime needs of Malaysians. The Company sees the importance of sustainable growth through global diversification, the reduction of complexity, value-based management approach, and crucially important employees, ultimately attaining customer satisfaction.


text:
young jamaican college gal living life to the fullest ^_^ i love fashion, music, and asian culture :*
I post alot of asia/jamaica related things :P
Language Spoken - Japanese, Chinese (Mandarin), Patwa, English
Music : UnsraW, Nega, Sel'm, Nocturnal Bloodlust, Dalth, ScReW, Born. Alice Nine. Di3irae, Deathgaze, meth. Lynch. Kyary Pamyu Pamyu,Kreva, Vybz Kartel, Aidonia, Spice, B2ST, MBLAQ....(yes thelist goes on)
if only I was in japa *_*
METO is badass
Royz <3 I love kazuki but in this picture he looks lost lol. Kuina, however is looking like a boss :D
Today I decided to deactivate one of my twitter accounts…obviously it was the one with the most followers. I was thinking of completely deleting it and keeping my backup which is my completely Asian based twitter (where I tweet in Japanese/English). This account have band members from jrock & j metalcore bands following me…if you ask me, that's better than having a bunch of thirst buckets following haha
her hair is perfect *-*
He is the cutest asian baby in the world . I want to kidnap him
My korean gangstas. I love their music


text:
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Heroes and marchesBy Ramon Farolan
Today people march all over the country to express their anger and indignation over pork;
Today people march all over the country to express their anger and indignation over pork;
Three years ago, I wrote a column titled "Two injustices, different endings." The first injustice had to do with the treatment of Filipino war veterans who served in the US Armed Forces at the outbreak of the Pacific War in 1941. Alongside US soldiers, the Filipinos fought with loyalty and determination even when the white […]
Notes on Philippine Military Academy homecoming activities: Vice President Jejomar Binay who also serves as the chairman of the Housing and Urban Development Coordinating Council (HUDCC) was the guest of honor at the PMA alumni gathering in Baguio City during the weekend. As I mentioned in an earlier column, Interior Secretary Mar Roxas was the guest speaker at the PMA Alumni Annual General Membership meeting held at Camp Aguinaldo last month. Roxas is an honorary member of PMA Class 1984 while Binay belongs to PMA Class 1998, members of whom are marking their Silver Jubilee this year.
Last week, Argentina became the first nation to be censured by the International Monetary Fund (IMF) because of doubts concerning the credibility of its economic data. Specifically the Washington-based lender alleged that President Cristina Fernandez de Kirchner's government is not addressing reports it understated inflation figures that analysts say are actually more than double the 10.8 percent official rate.
Notes on AFP Day. During the past few weeks, there have been rumors circulating about the health of President Aquino. These were brought about mainly by his inability to attend several important engagements here and abroad. He had to cancel a meeting with IMF chief Christine Lagarde during the latter's visit to the country last month. He also excused himself from the dinner hosted by New Zealand Prime Minister John Phillip Key in Wellington during his state visit. There were a number of Asean functions that he missed due to health concerns. Some doctors suggested that perhaps he needed a vacation.


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My talk of how much good doctors do
I little while ago I was invited to speak by Medsin Imperial and GWWC:London about how much good doctors do. Thanks to Sam Hilton and Maria Qursashi for hosting me, Gemma Hodgson for the video (although watching myself remains mortifying!) and to all those there who had no choice but to suffer me in the third person. Enjoy life!
2014/09/23 Leave a comment
Funding cannibalism motivates concern for overheads
Summary: Overhead expenses' (CEO salary, percentage spent on fundraising) are often deemed a poor measure of charity effectiveness by Effective Altruists, and so they disprefer means of charity evaluation which rely on these. However, 'funding cannibalism' suggests that these metrics (and the norms that engender them) have value: if fundraising is broadly a zero-sum game between charities, then there's a commons problem where all charities could spend less money on fundraising and all do more good, but each is locally incentivized to spend more. Donor norms against increasing spending on zero-sum 'overheads' might be a good way of combating this. This valuable collective action of donors may explain the apparent underutilization of fundraising by charities, and perhaps should make us cautious in undermining it.
2014/08/30 1 Comment
Why the tails come apart
Many outcomes of interest have pretty good predictors. It seems that height correlates to performance in basketball (the average height in the NBA is around 6'7″). Faster serves in tennis improve one's likelihood of winning. IQ scores are known to predict a slew of factors, from income, to chance of being imprisoned, to lifespan.
What is interesting is the strength of these relationships appear to deteriorate as you advance far along the right tail. Although 6'7″ is very tall, is lies within a couple of standard deviations of the median US adult male height – there are many thousands of US men taller than the average NBA player, yet are not in the NBA. Although elite tennis players have very fast serves, if you look at the players serving the fastest serves ever recorded, they aren't the very best players of their time. It is harder to look at the IQ case due to test ceilings, but again there seems to be some divergence near the top: the very highest earners tend to be very smart, but their intelligence is not in step with their income (their cognitive ability is around +3 to +4 SD above the mean, yet their wealth is much higher than this).1
The trend seems to be that although we know the predictors are correlated with the outcome, freakishly extreme outcomes do not go together with similarly freakishly extreme predictors. Why? continue reading »
- One might look at the generally modest achievements of people in high-IQ societies as further evidence, but there are worries about adverse selection. ↩
2014/07/22 1 Comment
Off duty
"Hi, single to the hospital, please."
"You're not ill, are ya?" The bus driver teased.
"No, no, I'm just training there."
He let her go with a laugh and she walked up the aisle of the bus. The white tunic with blue trim poking out from under her coat made me guess student nurse. I was next in the queue; the driver spared me the same joke when I asked for the same ticket.
I swung into my seat near the front, and put my newly bought laptop (long-awaited, and now looked-forward-to) on my lap. It was around seven, and the sky was in the throes of a stretched out summer sunset. The bus lurched forward, and my attention wandered.
"It's his stop!" continue reading »
2014/07/14 Leave a comment
How good were the old greats?
Summary: In many fields, the 'greatest' (be they philosophers, playwrights, composers, etc.) are selected disproportionately more from those who lived in the distant past. I speculate as to what might be driving this bias towards 'ancient greatness', but one important takeaway is that we can be confident that the greatest of the past are likely inferior to the greatest amongst us today in terms of 'innate ability'. So perhaps we should not regard them so highly.
[Very rough draft: advice/criticism on data, analysis, or style welcome, as is advice on whether this is worthy of going into academia, and if so how. Thanks to Rob Wiblin, Will Crouch, Catriona McKay, and Sam Bankman-Fried for ideas/prior discussion.]
2014/02/16 4 Comments
I am now a doctor. The result that I passed my finals came on Friday, and the declaration happened Sunday afternoon. I start work as the most junior of junior doctors – a Foundation Year 1 – at the end of July in Milton Keynes. So now you know where to avoid if you get sick in August.
Traditionally (at least in the 'States) students have a valedictory address at the end of their course, given by the top-ranked student of the year. This is a less auspicious farewell: I am a long way down any order of merit you care to name, and medical school has been a struggle. Things did get better in my final year, but the last six years haven't been some Bildungsroman of how I transformed from spotty student to modern medical professional. continue reading »
2013/07/01 Leave a comment
God, Evil, and Appearances: A Dialogue
[Published in THINK (2013; issue 12, pp 9-23)]
ADAM: Consider this: Neil and Kazumi Puttick, and their son Sam were, by all accounts, an idyllic family. One friend said: 'If you could bottle up a perfect marriage, theirs would be it'. They were involved in a car accident in 2005. Kazumi's legs and pelvis were broken. Sam – then 18 months old – had his spine severed at the neck. He would have died were it not for two doctors who happened to be passing by. After being rushed to hospital, Neil and Kazumi were told that Sam's injuries were catastrophic. Neil was defiant:
… I believe in my heart the doctors are wrong and he will win. I believe God is with us and Sam will walk, talk, and breathe again.
He was a miracle when came to us, it was a miracle when he survived the crash and it will be a miracle when he recovers. These things do happen and they will happen to Sam.
Sam survived, and although he didn't recover from paralysis, flourished in all other respects. Neil and Kazumi quit their jobs to devote their time looking after Sam and raising money for his care. The local community pitched in too: one of the things they did was take photographs of themselves from all over the world holding cards saying 'Hi Sam!' which Sam enjoyed immensely. Later the local government agreed to pay the costs of Sam's medical care. Neil and Kazumi continued their work, now directed towards raising awareness of spinal injuries. Sadly, the story doesn't end there.
Three years after the accident (just after he'd started at school) Sam contracted pneumococcal meningitis, a highly virulent and aggressive infection. Despite intensive care, it became clear there was no hope of survival. Neil and Kazumi took him back home, and he died shortly afterwards.
Beachy Head is a notorious suicide blackspot, so much so a chaplaincy has been set up expressly to patrol the cliffs and counsel those contemplating whether to jump. Despite this, no one saw two figures wearing rucksacks who leapt to their deaths. The bodies were discovered the following morning. They were Neil and Kazumi Puttick. Sam's body was in one their rucksacks; the other contained his toys.
The 'problem of evil' can mean many different things. It could be a moral problem: 'What should we do to stop the evil things in the world?' It could be a motive for existential crisis: 'How can we bear to live in a world with so much that is evil?' It could be an obstacle to religious faith: 'How can I love a God that lets these evil things occur?'
The sort of 'problem' I want to talk about is really an argument, that starts from the existence of evil, and ends up concluding that there is no God. Awful stories like the Putticks' are meant to demonstrate we do not live under the watchful benevolence of God, but rather in one of blind, pitiless indifference to our wellbeing. continue reading »
2013/03/19 4 Comments
Why you shouldn't believe the Resurrection happened
The 12th (and final) part in "20 Atheist answers to questions they supposedly can't."
- What accounts for the empty tomb, resurrection appearances and growth of the church?
Short answer: We shouldn't be that confident of these facts, but in any case the base rate fallacy and selection bias nixes the confirmatory power.
Longer answer: The argument implied in the question is that the historical record of Jesus provides strong evidence to believe he actually died and rose again, which provides evidence that Christianity's central claims (e.g. God exists, Jesus is the son of god) are true. The question neatly summarizes the three main 'planks' of evidence usually offered:
- The Empty Tomb. When Jesus died, his body was placed in a tomb. Not only was a stone rolled in front of it, but also the authorities posted sentries outside the tomb to stop anyone stealing the body. Despite this, the stone was discovered to be rolled away, and the body had gone. (e.g. Mark 16:4, Luke 24:2-3)
- Resurrection appearances. Several different groups of people (the disciples, some women, etc.) are reported to have seen Jesus after he died. (e.g. Luke 24:15-31, 36-48; Matthew 28:9-10)
- The growth of the church. After Jesus died, his apostles (and figures like Paul) were committed to the message of Jesus, and helped the church spread rapidly. (cf. Acts, but also the historical record re. the Holy Roman empire, etc.)
The idea is this data is very hard to explain via purely atheistic means. Maybe Jesus didn't really die, but is it plausible he could have got up and escaped the guarded tomb after being crucified and speared for good measure? Maybe the disciples managed to steal the body, but how did they manage to get that past the guards? (And what was in it for them? Why would many of them go on to die for a belief they knew to be false?) Maybe the appearances of the resurrection were just hallucinations, but how could there have been so many hallucinations, of so many different people, and why didn't the authorities just squash the story by presenting the public with Jesus's corpse?
So, it's argued, the best explanation for the historical data is the Christian one: Jesus rose from the dead and left the tomb miraculously, and then appeared to people like the apostles and women who visited the tomb, and these people, convinced by the truth, to go on and grow the church. continue reading »
2013/02/13 4 Comments
Unfriendly AI cannot be the Great Filter
[Slightly odd topic, originally posted on LessWrong.]
The Great Filter is the idea that although there is lots of matter, we observe no "expanding, lasting life", like space-faring intelligences. So there is some filter through which almost all matter gets stuck before becoming expanding, lasting life. One question for those interested in the future of humankind is whether we have already 'passed' the bulk of the filter, or does it still lie ahead? For example, is it very unlikely matter will be able to form self-replicating units, but once it clears that hurdle becoming intelligent and going across the stars is highly likely; or is it getting to a humankind level of development is not that unlikely, but very few of those civilizations progress to expanding across the stars. If the latter, that motivates a concern for working out what the forthcoming filter(s) are, and trying to get past them.
One concern is that advancing technology gives the possibility of civilizations wiping themselves out, and it is this that is the main component of the Great Filter – one we are going to be approaching soon. There are several candidates for which technology will be an existential threat (nanotechnology/'Grey goo', nuclear holocaust, runaway climate change), but one that looms large is Artificial intelligence (AI), and trying to understand and mitigate the existential threat from AI is the main role of the Singularity Institute, and I guess Luke, Eliezer (and lots of folks on LW) consider AI the main existential threat.
The concern with AI is something like this:
- AI will soon greatly surpass us in intelligence in all domains.
- If this happens, AI will rapidly supplant humans as the dominant force on planet earth.
- Almost all AIs, even ones we create with the intent to be benevolent, will probably be unfriendly to human flourishing.
Or, as summarized by Luke:
… AI leads to intelligence explosion, and, because we don't know how to give an AI benevolent goals, by default an intelligence explosion will optimize the world for accidentally disastrous ends. A controlled intelligence explosion, on the other hand, could optimize the world for good.
So, the aim of the game needs to be trying to work out how to control the future intelligence explosion so the vastly smarter-than-human AIs are 'friendly' (FAI) and make the world better for us, rather than unfriendly AIs (UFAI) which end up optimizing the world for something that sucks.
'Where is everybody?'
So, topic. I read this post by Robin Hanson which had a really good parenthetical remark (emphasis mine):
Yes, it is possible that the extremely difficultly was life's origin, or some early step, so that, other than here on Earth, all life in the universe is stuck before this early extremely hard step. But even if you find this the most likely outcome, surely given our ignorance you must also place a non-trivial probability on other possibilities. You must see a great filter as lying between initial planets and expanding civilizations, and wonder how far along that filter we are. In particular, you must estimate a substantial chance of "disaster", i.e., something destroying our ability or inclination to make a visible use of the vast resources we see. (And this disaster can't be an unfriendly super-AI, because that should be visible.)
This made me realize an UFAI should also be counted as an 'expanding lasting life', and should be deemed unlikely by the Great Filter.
Another way of looking at it: if the Great Filter still lies ahead of us, and a major component of this forthcoming filter is the threat from UFAI, we should expect to see the UFAIs of other civilizations spreading across the universe (or not see anything at all, because they would wipe us out to optimize for their unfriendly ends). That we do not observe it disconfirms this conjunction. 1
A much more in-depth article and comments (both highly recommended) was made by Katja Grace a couple of years ago. I can't seem to find a similar discussion on here (feel free to downvote and link in the comments if I missed it), which surprises me: I'm not bright enough to figure out the anthropics, and obviously one may hold AI to be a big deal for other-than-Great-Filter reasons (maybe a given planet has a 1 in a googol chance of getting to intelligent life, but intelligent life 'merely' has a 1 in 10 chance of successfully navigating an intelligence explosion), but this would seem to be substantial evidence driving down the proportion of x-risk we should attribute to AI.
What do you guys think?
- It also gives a stronger argument – as the UFAI is the 'expanding life' we do not see, the beliefs, 'the Great Filter lies ahead' and 'UFAI is a major existential risk' lie opposed to one another: the higher your credence in the filter being ahead, the lower your credence should be in UFAI being a major existential risk (as the many civilizations like ours that go on to get caught in the filter do not produce expanding UFAIs, so expanding UFAI cannot be the main x-risk); conversely, if you are confident that UFAI is the main existential risk, then you should think the bulk of the filter is behind us (as we don't see any UFAIs, there cannot be many civilizations like ours in the first place, as we are quite likely to realize an expanding UFAI). ↩
2012/12/22 Leave a comment
Atheist Prayer Experiment: Conclusion
Several weeks late, but never mind.
I was another one of those who got no answer, despite my best efforts. If anything, my life was marginally less numinous than usual: nothing resembling spiritual longing, my life was slightly more fraught and disappointing than usual during the 'prayer experiment' (although nothing dramatic).
You can probably guess what I'm going to take from this based on my earlier posts: a negative answer is good (further) evidence for Atheism, as I'm pretty confident I behaved in a manner such that God (if he was really there) would get in touch. Yet he hasn't.
In medical school you are taught to only order tests you plan to act on. Although this principle isn't watertight (sometimes you will only act on particular results of a test, for example), it is a good heuristic. So my action plan now is to spend less time on philosophy of religion: my evaluation of the merits of various arguments are now pretty stable, so I don't anticipate further study will be that fruitful in terms of value of information – there might be some killer argument to change my mind, but I don't hold out much hope for it. In contrast, the concerns around ethics and epistemology (like the ethics of charity, what should be prioritized, how to consider future generations) seem like problems worth more of my time as theism fades into unlikelihood.
I'll still remain open to divine revelation, of course: it makes no sense to try and close oneself off from potential data. But I'll mostly have given up looking – if it arrives regardless, well and good, but I have bigger problems than to keep waiting for a (now very delayed) damascene moment. I think Mawson's analogy would agree with me: if you're told a man is in a dark room who is worth talking to, yet you shout out 40 or so times and get no reply, you might keep an ear out, but you won't keep shouting 'just in case' he decides to finally respond.
I remain hopeful that some God exists – it would make the world a better place, at least for those who suffer. But hope isn't expectation, and I do not anticipate this hope will be fulfilled. In the meanwhile, it seems best to live ones life with care to try and make the world a better place, especially for those rendered the worst off. Take care of that, and hope Marcus Aurelius is on the right track:
Live a good life. If there are gods and they are just, then they will not care how devout you have been, but will welcome you based on the virtues you have lived by. If there are gods, but unjust, then you should not want to worship them. If there are no gods, then you will be gone, but will have lived a noble life that will live on in the memories of your loved ones.
Enjoy life,
2012/11/28 1 Comment


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BOW DOWN to the Beyonce-inspired childhood photo meme. Take a look at the best of the best here.
HyperVocal Halloween: On the left, HV's Cooper Fleishman channels Paul Ryan. On the right, HV's Greg Seals just blue himself as Tobias Funke.
This analrapist just blue himself. Oh, Tobias, you blowhard!
A 16-year-old San Antonio student accused of sharing a nude cellphone pic of a minor swallowed the phone's memory card during a conversation with the vice principal. He's since been arrested, released and assigned to a new school. HyperVentilate chief Greg Seals sounds off.
Queen Elizabeth II's royal corgis got into a fight this weekend. Now it seems like they've found a new career. Lord Greg Seals throws down the challenge.
Grindr CEO Joel Simkhai sits down with HyperVocal's Greg Seals for a fun and interesting discussion about the gay hook-up app's history, its goals, its future and its backlash. Plus, he plays 'F*ck, Marry, Kill" with three weird options.
10 Things I Did While Twitter Was Down
1. Chopped up all the furniture in my house; built a fire to stay warm
2. Wrote my tweets on tiny sheets of paper and tied them to the leg of a raven to fly them straight to Twitter HQ
3. Followed strangers to their cars in the parking lot of Target asking if they were part of #TeamFollowBack
4. Buried my dog. I had forgotten to feed him since the last time I logged into Twitter 21 days ago
5. Took my first shower in a week. Mainly so I could cry and mask my tears
6. Read the backlog of tweets I printed out and stored in a bank lock box
7. Faxed all my celebrity death jokes to the obituary section of my local paper
8. Tracked down and reunited with my birth parents
9. Hunted, skinned and cooked my own DiGiorno pizza
10. Waved a white flag on my roof waiting for FEMA assistance
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Netflix's stock took a huge hit today. WILL IT GO AWAY FOREVER? Greg Seals is worried he may have to go for a hike now. Uh oh.
Greg Seals on the Season 5 premiere premiere: "Congrats to white males under 65, you can stop pretending to like Newsroom, Breaking Bad is back."


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Ghost Adventures: Saint James Hotel Pictures
Zak, Nick and Aaron travel to the St. James Hotel in New Mexico, which once hosted Wild West legends like Jesse James and Buffalo Bill.Filed under:
Zak Bagans checks his notes before the guys start filming in front of the St. James Hotel in Cimarron, NM.
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Three years ago, Linda Morse tossed a tennis ball gracefully overhead, reached for it with her racket, planted her left leg on the hard tennis court for leverage, and felt something snap. "It was one of those 'Oh, no' moments," says Morse, now 73. "Most people I know who play tennis have some kind of knee trouble, and now, I just knew, I did, too." … Back to Article
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With Quercetin & NAC
These statements have not been evaluated by the Food and Drug Administration (FDA). These products are not meant to diagnose' treat or cure any disease or medical condition. Please consult your doctor before starting any exercise or nutritional supplement program or before using these or any product during pregnancy or if you have a serious medical condition.
Serving Size: 2 capsules
Serving Per Container: 25
Amount Per Serving % Daily Value
Vitamin A (as retinyl palmitate) 2'500 I.U 50 %
Vitamin C (as calcium ascorbate) 250 mg 417 %
Pantothenic Acid (as d-calcium pantothenate) 200 mg 2'000 %
Zinc (as zinc monomethionine) 10 mg 67 %
Quercetin (from Sopohorae japonica linn) (flower) 250 mg *
N-Acetyl Cysteine 200 mg *
L-Histidine (free form) 200 mg *
Bromelain (2'000 GDU/G)(from pineapple) 100 mg *
Stinging Nettle (leaf) 100 mg *
Cayenne (fruit) 20 mg *
Grape Seed Extract 5 mg *
(95% polyphenols equal to 4.75 mg)
* Daily Value not established.
Other Ingredients: Cellulose (capsule shell)' magnesium stearate' silica.
(Does Not Contain: Yeast' wheat' soy' gluten' milk' salt' or artificial color.)
Adults take two (2) capsules daily or as directed by a health care professional.
As a reminder' discuss the supplements and medications that you take with your health care providers.
If you are pregnant or nursing' have a gastrointestinal?disorder' are taking medication or planning a surgery' consult your doctor before using this product.
If any adverse reactions occur' stop taking the product and consult your doctor.
Allergy / Dietary Needs
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- Vegetarian Product
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Overall Rating for Aller-Max 100 Vegetarian Capsules
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Allergies under control
I had been looking for a product that would help keep me off prescription allergy medications and a friend mentioned I should try quercetin. Ive been using this product for several years now. It has helped keep my allergies under control. I like to spend time outdoors so I take it regularly. I also love that it contains NAC!
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Allergy relief!
Initially I bought Aller-Max at a vitamin store chain. A friend recommended it to me for allergy relief and has helped to relieve congestion and coughing I was having with seasonal allergies. I bought my second bottle at pureformulas for almost $15 less. It s a great price and product. I started taking 2 in am and 2 in pm and then tapered off to one in am and one before bed. Easy to take capsules!
√ I would recommend this item to a friend.
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Conn Creek Anthology 2008
Bordeaux Red Blends from Napa Valley, California
Winemaker's Notes
Berries, violets, allspice and freshly baked pastry unite in an offering reminiscent of berry pie. The aromatic potpourri prominently features bright cherry flavors and sandalwood-tinged cocoa spice. Handsomely sculptured tannins showcase the mouthwatering fruit compote in the long, lingering finish.
Blend: 66% Cabernet Sauvignon, 12% Malbec, 11% Merlot, 7% Petite Verdot, 4% Cabernet Franc
Critical Acclaim
Wine & Spirits - "This takes a fresh approach to classical Napa Valley cabernet, a sleek, cool and delicious red, layering scents of violets, berries and oak. There's a resonant complexity to its depth of flavor, a full-on ripeness with no hint of raisining. Mike McGrath blends this from a wide range of vineyard sources throughout Napa Valley, including Stagecoach on Atlas Peak, Truchard in Carneros and Frediani in Calistoga. The wine is mainly cabernet sauvignon (66 percent), along with malbec, merlot and smaller amounts of petite verdot and franc, the combination supple and very easy to drink. "
Conn Creek Winery
Conn Creek was founded in 1973 and immediately established itself as one of Napa Valley's premier Cabernet houses with the release of its 1974 Eisele Vineyard Cabernet Sauvignon. The winery name comes from its location, next to Conn Creek near the Rutherford Crossroad that connects the Silverado Trail with Highway 29. Conn Creek specializes in limited production Bordeaux varietal wines exclusively from Napa Valley's best vineyards. Present production remains at fewer than 6500 case per year. View all Conn Creek Winery Wines
About Napa ValleyView a map of Napa Valley wineries
It's hard not to think of Napa Valley when thinking of California wines. The region is, after all, the one that brought world recognition to California wine making. The area was settled by a few choice wine families in the 1960's who bet that the wines of the area would grow and flourish. They were right. The Napa wine industry really took off in the 1980's, when vineyard lands were scooped up and vines were planted throughout the county. A number of wineries emerged, from large conglomerates to small boutiques to cult classics. Cabernet Sauvignon is definitely the grape of choice here, with many winemakers also focusing on Bordeaux Blends. Whites are usually Chardonnay and Sauvignon Blanc.
Notable FactsWithin the Napa Valley lie smaller sub-AVAs that lend even more character specifics to the wines. Furthest south is Carneros, followed by Yountville, Oakville & Rutherford. Above those two is St.-Helena and finally, just grated an AVA, Calistoga. These areas are situated on the valley floor and are known for creating rich, smooth Cabernet Sauvignon, Merlot and Chardonnay. There are a few mountain regions as well, nestled on the slopes overlooking the valley AVAs. Those include Howell Mountain, Stags Leap and Mount Veeder. Wines from the mountain regions are often more structured and firm, benefiting from more time in the bottle to evolve and soften.
About CaliforniaIt's not rare to see a wine's country of origin listed as "California." A country into itself in the wine world, California makes enough varieties and styles to match many European wine countries. It produces a diverse range of wines that span the quality spectrum.
The most famous of the California wine regions is Napa Valley, and these wines are certainly outstanding – but it's not as broad and diverse as its larger neighbor, Sonoma County. Down south, Santa Barbara's Santa Maria Valley is well-known for its Rhône blends, as well as cool-climate varieties like Pinot and Chardonnay. The Central Coast, the largest California AVA, has many different microclimates that lead to a wide range of wines with many sub-AVAs.
Customer ReviewsSign In to Add Your Review0 }div>Related ProductsThe debut release of the Quilceda Creek 2006 Palengat Vineyard Cabernet Sauvignon displays the wonderful aromatic perfume of the Horse ...Our 2010 Platinum delivers rich elegance cloaked in a dark profile. Opulent, brooding and incredibly black in every sense. Blackberry, ...The 2010 The Mariner is representative of the cooler vintage with moderate temperatures. Initial aromatics speak to those cool vintage ...
Alcohol By Volume GuideMost wine ranges from 10-16% alcohol by volume. Some varietals tend to have higher (for example Zinfandel and Cabernet Sauvignon) or lower alcohol levels (Pinot Noir and many white varietals), but there is always some variation from producer to producer. Some wine falls outside of this range, for instance Port weighs in closer to 20%, while Muscat and Riesling are usually a bit below 10%.
Wine Style Guide
Light & Fruity
- Red wines that are more fruit-forward and lighter in tannin and body.
Smooth & Supple
- Medium bodied reds that go down easy, with smooth tannins and supple fruit.
Earthy & Spicy
- Wines where earthy and/or spicy dominate the flavors – typically medium to full body.
Big & Bold
- Full bodied wines that have concentrated fruit and are higher in alcohol and/or tannins. Some need age.


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Free Training: Sound Design Intensive. 3 Classes To Create Amazing Sounds
In this FREE 6 hour workshop, I will go in depth into specific methods used to create amazing patches. Split into three 2 hour sessions, each session is focused on a specific kind of sound. At the end of the day, all attendees will have what they need to go and create amazing pads, pianos, and synths on their workstation – be it software such as Reason, Omnisphere, or Logic… or hardware such as Nord Stages, Korg Kronos or Yamaha Motifs.
The entire workshop is streamed LIVE from my home studio. I use my computer to show real examples that are applicable to nearly any skill level, as well as any "class" of gear. I focus on giving real CONTEXT in order to help you understand how different concepts will apply in the real world.
Who should attend: This workshop is applicable to both studio musicians and live performers. Though not necessary, I would encourage attendees to have watched my Keyboard And Synth Bootcamp – currently available for purchase on my website. Any skill level from Beginner (what's a synth) to Advanced (owns 50 keyboards and soft synths) will find something to get out of this free workshop and are encouraged to attend.


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General Process of Creating Java Code Examples (Using Eclipse)
Download and install the AWS Toolkit for Eclipse. This toolkit includes the AWS SDK for Java, along with preconfigured templates for building applications.
From the Eclipse menu, click File, New, Other...
In the Select a wizard box, click File, AWS Java Project, Click Next.In the Project name field, type a name for your project. Click Finish to create the project. Note that the project is pre-configured, and includes the AWS SDK for Java .jar files.
You will now need to create a default credential profiles file. This file enhances security by storing your credentials separately from your project directories, so that they cannot be unintentionally committed to a public repository. For more information, see Using the Default Credential Provider Chain in the AWS SDK for Java Developer Guide.The credential properties file should be saved as
~/.aws/credentials, where the tilde character
represents your home directory. In this file, you can store multiple
sets of credentials from any number of accounts. Each set is referred to
as a profile. The following is an example of a credential properties
file with a a profile named
[default] aws_access_key_id =
The code examples in this document use the default client constructors that read your AWS credentials stored in the credential properties file.
Copy the code from the section that you are reading to your project.
Run the code.
You can set the DynamoDB region explicitly, as shown in the following Java code snippet.
client = new AmazonDynamoDBClient(credentials); client.setRegion(Region.getRegion(Regions.US_WEST_2));
For a current list of supported regions and endpoints, see Regions and Endpoints.


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Refolding of proteins from inclusion bodies: rational design and recipes
- Anindya Basu,
- Xiang Li,
- Susanna Su Jan Leong
- … show all 3 hide
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The need to develop protein biomanufacturing platforms that can deliver proteins quickly and cost-effectively is ever more pressing. The rapid rate at which genomes can now be sequenced demands efficient protein production platforms for gene function identification. There is a continued need for the biotech industry to deliver new and more effective protein-based drugs to address new diseases. Bacterial production platforms have the advantage of high expression yields, but insoluble expression of many proteins necessitates the development of diverse and optimised refolding-based processes. Strategies employed to eliminate insoluble expression are reviewed, where it is concluded that inclusion bodies are difficult to eliminate for various reasons. Rational design of refolding systems and recipes are therefore needed to expedite production of recombinant proteins. This review article discusses efforts towards rational design of refolding systems and recipes, which can be guided by the development of refolding screening platforms that yield both qualitative and quantitative information on the progression of a given refolding process. The new opportunities presented by light scattering technologies for developing rational protein refolding buffer systems which in turn can be used to develop new process designs armed with better monitoring and controlling functionalities are discussed. The coupling of dynamic and static light scattering methodologies for incorporation into future bioprocess designs to ensure delivery of high-quality refolded proteins at faster rates is also discussed.
Within this Article
- Introduction
- Inclusion body formation: can it be avoided?
- Design rationale of refolding methods
- Light scattering methods: a rational approach to designing protein refolding recipes
- Future directions: using light scattering tools for rational design of refolding processes
- Concluding remarks
- References
- References
Other actions
- Anselment B, Baerend D, Mey E, Buchner J, Weuster-Botz D, Haslbeck M (2010) Experimental optimization of protein refolding with a genetic algorithm. Protein Sci 19(11):2085–2095
- Basu A, Chen WN, Leong SSJ (2011) A rational design for hepatitis B virus X protein refolding and bioprocess development guided by second virial coefficient studies. Appl Microbiol Biot 90(1):181–191 CrossRef
- Bellott E (2010) The rising tide of biotech. Eur Biopharm Rev (Autumn):92–97
- Benoit I, Coutard B, Oubelaid R, Asther M, Bignon C (2007) Expression in Escherichia coli, refolding and crystallization of Aspergillus niger feruloyl esterase A using a serial factorial approach. Protein Expres Purif 55(1):166–174
- Braun P, Hu Y, Shen B, Halleck A, Koundinya M, Harlow E, LaBaer J (2002) Proteome-scale purification of human proteins from bacteria. Proc Natl Acad Sci USA 99(5):2654–2659 CrossRef
- Bryksa BC, MacDonald LD, Patrzykat A, Douglas SE, Mattatall NR (2006) A C-terminal glycine suppresses production of pleurocidin as a fusion peptide in Escherichia coli. Protein Expres Purif 45(1):88–98 CrossRef
- Burgess-Brown NA, Sharma S, Sobott F, Loenarz C, Oppermann U, Gileadi O (2008) Codon optimization can improve expression of human genes in Escherichia coli: a multi-gene study. Protein Expres Purif 59(1):94–102 CrossRef
- Cao S, Pollastrini J, Jiang Y (2009) Separation and characterization of protein aggregates and particles by field flow fractionation. Curr Pharm Biotechnol 10(4):382–390 CrossRef
- Chen Y, Leong SSJ (2009) Adsorptive refolding of a highly disulfide-bonded inclusion body protein using anion-exchange chromatography. J Chromatogr A 1216(24):4877–4886 CrossRef
- Chen Y, Leong SSJ (2010) High productivity refolding of an inclusion body protein using pulsed-fed size exclusion chromatography. Process Biochem 45(9):1570–1576 CrossRef
- Chuan YP, Fan YY, Lua L, Middelberg APJ (2008a) Quantitative analysis of virus-like particle size and distribution by field-flow fractionation. Biotechnol Bioeng 99(6):1425–1433 CrossRef
- Chuan YP, Lua LHL, Middelberg APJ (2008b) High-level expression of soluble viral structural protein in Escherichia coli. J Biotechnol 134(1–2):64–71 CrossRef
- Cowan RH, Davies RA, Pinheiro TTJ (2008) A screening system for the identification of refolding conditions for a model protein kinase, p38α. Anal Biochem 376(1):25–38
- Crisman RL, Randolph TW (2009) Refolding of proteins from inclusion bodies is favored by a diminished hydrophobic effect at elevated pressures. Biotechnol Bioeng 102(2):483–492 CrossRef
- Curtis RA, Lue L (2006) A molecular approach to bioseparations: protein–protein and protein–salt interactions. Chem Eng Sci 61(3):907–923 CrossRef
- Curtis RA, Ulrich J, Montaser A, Prausnitz JM, Blanch HW (2002) Protein–protein interactions in concentrated electrolyte solutions. Biotechnol Bioeng 79(4):367–380. doi:10.1002/bit.10342 CrossRef
- Dechavanne V, Barrillat N, Borlat F, Hermant A, Magnenat L, Paquet M, Antonsson B, Chevalet L (2011) A high-throughput protein refolding screen in 96-well format combined with design of experiments to optimize the refolding conditions. Protein Expres Purif 75(2):192–203
- de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B (2007) Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli. BMC Biotechnol 7:32 CrossRef
- Demain AL, Vaishnav P (2009) Production of recombinant proteins by microbes and higher organisms. Biotechnol Adv 27(3):297–306 CrossRef
- Deshpande K, Ahamed T, Van Der Wielen LAM, Ter Horst JH, Jansens PJ, Ottens M (2009) Protein self-interaction chromatography on a microchip. Lab Chip—Miniaturisation for Chemistry and Biology 9(4):600–605 CrossRef
- Dobson CM (2003) Protein folding and misfolding. Nature 426(6968):884–890 CrossRef
- Eisenberg D, Marcotte EM, Xenarios I, Yeates TO (2000) Protein function the post-genomic era. Nature 405(6788):823–826 CrossRef
- Ejima D, Ono K, Tsumoto K, Arakawa T, Eto Y (2006) A novel "reverse screening" to identify refolding additives for activin-A. Protein Expres Purif 47(1):45–51
- Fahnert B, Lilie H, Neubauer P (2004) Inclusion bodies: formation and utilisation. Adv Biochem Eng/Biotechnol 89:93–142
- Fang L, Xia B, Inouye M (1999) Transcription of cpsA, the gene for the major cold-shock protein of Escherichia coli, is negatively regulated at 37°C by the 5′-untranslated region of its mRNA. FEMS Microbiol Lett 176(1):39–43
- Gast K, Nöppert A, Müller-Frohne M, Zirwer D, Damaschun G (1997) Stopped-flow dynamic light scattering as a method to monitor compaction during protein folding. Eur Biophys J 25(3):211–219. doi:10.1007/s002490050033 CrossRef
- George A, Wilson WW (1994) Predicting protein crystallization from a dilute solution property. Acta Crystallogr D 50:361–365 CrossRef
- Georgiou G, Valax P (1996) Expression of correctly folded proteins in Escherichia coli. Curr Opin Biotech 7(2):190–197 CrossRef
- Greenshields AL, Knickle LC, Syvitski R, Douglas SE (2008) Strategies for recombinant expression of small, highly disulphide-bonded, cationic antimicrobial peptides. Protein Peptide Lett 15(9):985–994 CrossRef
- Grigsby JJ, Blanch HW, Prausnitz JM (2000) Diffusivities of lysozyme in aqueous MgCl2 solutions from dynamic light-scattering data: effect of protein and salt concentrations. J Phys Chem B 104(15):3645–3650 CrossRef
- Guo B, Kao S, McDonald H, Asanov A, Combs LL, Wilson WW (1999) Correlation of second virial coefficients and solubilities useful in protein crystal growth. J Cryst Growth 196(2–4):424–433 CrossRef
- Ho JGS, Middelberg APJ (2004) Estimating the potential refolding yield of recombinant proteins expressed as inclusion bodies. Biotechnol Bioeng 87(5):584–592 CrossRef
- Ho JGS, Middelberg APJ, Ramage P, Kocher HP (2003) The likelihood of aggregation during protein renaturation can be assessed using the second virial coefficient. Protein Sci 12(4):708–716 CrossRef
- Hoffmann F, Rinas U (2004) Stress induced by recombinant protein production in Escherichia coli. Adv Biochem Eng/Biotechnol 89:73–92
- Huang L, Ching CB, Jiang R, Leong SSJ (2008) Production of bioactive human beta-defensin 5 and 6 in Escherichia coli by soluble fusion expression. Protein Expres Purif 61(2):168–174 CrossRef
- Huang L, Leong SSJ, Jiang R (2009) Soluble fusion expression and characterization of bioactive human beta-defensin 26 and 27. Appl Microbiol Biot 84(2):301–308 CrossRef
- Jungbauer A, Kaar W (2007) Current status of technical protein refolding. J Biotechnol 128(3):587–596 CrossRef
- Jungbauer A, Kaar W, Schlegl R (2004) Folding and refolding of proteins in chromatographic beds. Curr Opin Biotech 15(5):487–494 CrossRef
- Kaar W, Hartmann BM, Fan Y, Zeng B, Lua LHL, Dexter AF, Falconer RJ, Middelberg APJ (2009) Microbial bio-production of a recombinant stimuli-responsive biosurfactant. Biotechnol Bioeng 102(1):176–187 CrossRef
- Kirschner A, Altenbuchner J, Bornscheuer UT (2007) Cloning, expression, and characterization of a Baeyer–Villiger monooxygenase from Pseudomonas fluorescens DSM 50106 in E. coli. Appl Microbiol Biot 73(5):1065–1072 CrossRef
- Kleber-Janke T, Becker WM (2000) Use of modified BL21(DE3) Escherichia coli cells for high-level expression of recombinant peanut allergens affected by poor codon usage. Protein Expres Purif 19(3):419–424 CrossRef
- Komar AA (2009) A pause for thought along the co-translational folding pathway. Trends Biochem Sci 34(1):16–24 CrossRef
- Krasheninnikov IA, Komar AA, Adzhubeǐ IA (1988) Rol' klasterov redkikh kodonov v opredenelenii granits uchastkov polipeptidnoǐ tsepi s odnotipnoǐ vtoprichnoǐ strukturoǐ v protsesse ko-transliatsionnogo svorachivaniia belka [Role of the rare codon clusters in defining the boundaries of polypeptide chain regions with identical secondary structures in the process of co-translational folding of proteins]. Dokl Akad Nauk SSSR 303(4):995–999
- Kuehner DE, Heyer C, Rämsch C, Fornefeld UM, Blanch HW, Prausnitz JM (1997) Interactions of lysozyme in concentrated electrolyte solutions from dynamic light-scattering measurements. Biophys J 73(6):3211–3224 CrossRef
- Kulkarni A, Zukoski C (2001) Depletion interactions and protein crystallization. J Cryst Growth 232(1–4):156–164 CrossRef
- Kulkarni AM, Chatterjee AP, Schweizer KS, Zukoski CF (2000) Effects of polyethylene glycol on protein interactions. J Chem Phys 113(21):9863–9873 CrossRef
- Langenhof M, Leong SSJ, Pattenden LK, Middelberg APJ (2005) Controlled oxidative protein refolding using an ion-exchange column. J Chromatogr A 1069(2):195–201 CrossRef
- Leong SSJ, Chen WN (2008) Preparing recombinant single chain antibodies. Chem Eng Sci 63(6):1401–1414 CrossRef
- Leong SSJ, Middelberg APJ (2006) Dilution versus dialysis: a quantitative study of the oxidative refolding of recombinant human alpha-fetoprotein. Food Bioprod Process 84(1 C):9–17 CrossRef
- Leong SSJ, Middelberg APJ (2007) A simplified bioprocess for human alpha-fetoprotein production from inclusion bodies. Biotechnol Bioeng 97(1):99–117 CrossRef
- Li X, Leong SSJ (2011) A chromatography-focused bioprocess that eliminates soluble aggregation for bioactive production of a new antimicrobial peptide candidate. J Chromatogr A 1218(23):3654–3659 CrossRef
- Li M, Su ZG, Janson JC (2004) In vitro protein refolding by chromatographic procedures. Protein Expres Purif 33(1):1–10 CrossRef
- Lin L, Seehra J, Stahl ML (2006) High-throughput identification of refolding conditions for LXRβ without a functional assay. Protein Expres Purif 47(2):355–366
- Liu W, Bratko D, Prausnitz JM, Blanch HW (2004) Effect of alcohols on aqueous lysozyme–lysozyme interactions from static light-scattering measurements. Biophys Chem 107(3):289–298 CrossRef
- Liu W, Cellmer T, Keerl D, Prausnitz JM, Blanch HW (2005) Interactions of lysozyme in guanidinium chloride solutions from static and dynamic light-scattering measurements. Biotechnol Bioeng 90(4):482–490 CrossRef
- Liu X, Du Y, Guo Z, Gunasekaran S, Ching CB, Chen Y, Leong SSJ, Yang Y (2009) Monodispersed MCM-41 large particles by modified pseudomorphic transformation: direct diamine functionalization and application in protein bioseparation. Micropor Mesopor Mat 122(1–3):114–120 CrossRef
- Luo J, Leeman M, Ballagi A, Elfwing A, Su Z, Janson JC, Wahlund KG (2006) Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation-multi-angle light scattering. J Chromatogr A 1120(1–2):158–164 CrossRef
- Malavasi NV, Foguel D, Bonafe CFS, Braga CACA, Chura-Chambi RM, Vieira JM, Morganti L (2011) Protein refolding at high pressure: optimization using eGFP as a model. Process Biochem 46(2):512–518 CrossRef
- Mansell TJ, Fisher AC, DeLisa MP (2008) Engineering the protein folding landscape in gram-negative bacteria. Curr Protein Pept Sci 9(2):138–149 CrossRef
- Marin M (2008) Folding at the rhythm of the rare codon beat. Biotechnol J 3(8):1047–1057 CrossRef
- Mark JK, Smith S, Hefford MA (2007) Over-expression and refolding of MAP kinase phosphatase 3. Protein Expres Purif 54(2):253–260
- Martin C, Lenhoff AM (2011) Self-interaction chromatography of proteins on a microfluidic monolith. Biochem Eng J 53(2):216–222 CrossRef
- Martínez-Alonso M, García-Fruitós E, Ferrer-Miralles N, Rinas U, Villaverde A (2010) Side effects of chaperone gene co-expression in recombinant protein production. Microb Cell Fact 9:64 CrossRef
- Masip L, Pan JL, Haldar S, Penner-Hahn JE, DeLisa MP, Georgiou G, Bardwell JCA, Collet JF (2004) An engineered pathway for the formation of protein disulfide bonds. Science 303(5661):1185–1189 CrossRef
- Meechai N, Jamieson AM, Blackwell J (1999) Translational diffusion coefficients of bovine serum albumin in aqueous solution at high ionic strength. J Colloid Interf Sci 218(1):167–175 CrossRef
- Middelberg APJ (2002) Preparative protein refolding. Trends Biotechnol 20(10):437–443 CrossRef
- Mirarefi AY, Zukoski CF (2004) Gradient diffusion and protein solubility: use of dynamic light scattering to localize crystallization conditions. J Cryst Growth 265(1–2):274–283 CrossRef
- Muschol M, Rosenberger F (1995) Interactions in undersaturated and supersaturated lysozyme solutions: static and dynamic light scattering results. J Chem Phys 103(24):10424–10432 CrossRef
- Neal BL, Asthagiri D, Lenhoff AM (1998) Molecular origins of osmotic second virial coefficients of proteins. Biophys J 75(5):2469–2477 CrossRef
- Nishihara K, Kanemori M, Kitagawa M, Yanagi H, Yura T (1998) Chaperone coexpression plasmids: differential and synergistic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of Japanese cedar pollen, Cryj2, in Escherichia coli. Appl Environ Microb 64(5):1694–1699
- Pan X, Glatz CE (2003) Solvent effects on the second virial coefficient of subtilisin and solubility. Crystal Growth and Design 3(2):203–207 CrossRef
- Pazgier M, Lubkowski J (2006) Expression and purification of recombinant human α-defensins in Escherichia coli. Protein Expres Purif 49(1):1–8 CrossRef
- Phadtare S, Severinov K (2005) Extended-10 motif is critical for activity of the cspA promoter but does not contribute to low-temperature transcription. J Bacteriol 187(18):6584–6589 CrossRef
- Pizarro SA, Dinges R, Adams R, Sanchez A, Winter C (2009) Biomanufacturing process analytical technology (PAT) application for downstream processing: using dissolved oxygen as an indicator of product quality for a protein refolding reaction. Biotechnol Bioeng 104(2):340–351 CrossRef
- Prinsen P, Odijk T (2007) Collective diffusion coefficient of proteins with hydrodynamic, electrostatic, and adhesive interactions. J Chem Phys 127(11)
- Purvis IJ, Bettany AJE, Santiago TC, Coggins JR, Duncan K, Eason R, Brown AJP (1987) The efficiency of folding of some proteins is increased by controlled rates of translation in vivo. A hypothesis. J Mol Biol 193(2):413–417 CrossRef
- Qoronfleh MW, Hesterberg LK, Seefeldt MB (2007) Confronting high-throughput protein refolding using high pressure and solution screens. Protein Expres Purif 55(2):209–224 CrossRef
- Radford SE (2000) Protein folding: progress made and promises ahead. Trends Biochem Sci 25(12):611–618 CrossRef
- Read EK, Park JT, Shah RB, Riley BS, Brorson KA, Rathore AS (2010) Process analytical technology (PAT) for biopharmaceutical products: part I. Concepts and applications. Biotechnol Bioeng 105(2):276–284 CrossRef
- Roda B, Zattoni A, Reschiglian P, Moon MH, Mirasoli M, Michelini E, Roda A (2009) Field-flow fractionation in bioanalysis: a review of recent trends. Anal Chim Acta 635(2):132–143 CrossRef
- Rosenbaum DF, Zukoski CF (1996) Protein interactions and crystallization. J Cryst Growth 169(4):752–758 CrossRef
- Rubin J, Miguel AS, Bommarius AS, Behrens SH (2010) Correlating aggregation kinetics and stationary diffusion in protein-sodium salt systems observed with dynamic light scattering. J Phys Chem B 114(12):4383–4387 CrossRef
- Ruppert S, Sandler SI, Lenhoff AM (2001) Correlation between the osmotic second virial coefficient and the solubility of proteins. Biotechnol Prog 17(1):182–187 CrossRef
- Scheich C, Niesen FH, Seckler R, Büssow K (2004) An automated in vitro protein folding screen applied to a human dynactin subunit. Protein Sci 13(2):370–380
- Schmoeger E, Wellhoefer M, Dürauer A, Jungbauer A, Hahn R (2010) Matrix-assisted refolding of autoprotease fusion proteins on an ion exchange column: a kinetic investigation. J Chromatogr A 1217(38):5950–5956. doi:10.1016/j.chroma.2010.07.053 CrossRef
- Seefeldt MB, Rosendahl MS, Cleland JL, Hesterberg LK (2009) Application of high hydrostatic pressure to dissociate aggregates and refold proteins. Curr Pharm Biotechnol 10(4):447–455 CrossRef
- Shuler ML, Kargı F (2002) Bioprocess engineering: basic concepts. Prentice-Hall international series in the physical and Chem Eng Scis, 2nd edn. Prentice Hall, Upper Saddle River
- Stockmayer WH (1950) Light scattering in multi-component systems. J Chem Phys 18(1):58–61 CrossRef
- Tessier PM, Lenhoff AM (2003) Measurements of protein self-association as a guide to crystallization. Curr Opin Biotech 14(5):512–516 CrossRef
- Trésaugues L, Collinet B, Minard P, Henckes G, Aufrère R, Blondeau K, Liger D, Zhou CZ, Janin J, Van Tilbeurgh H, Quevillon-Cheruel S (2004) Refolding strategies from inclusion bodies in a structural genomics project. J Struct Funct Genomics 5(3):195–204
- Tsumoto K, Ejima D, Kumagai I, Arakawa T (2003) Practical considerations in refolding proteins from inclusion bodies. Protein Expres Purif 28(1):1–8 CrossRef
- Tsumoto K, Arakawa T, Chen L (2010) Step-wise refolding of recombinant proteins. Curr Pharm Biotechnol 11(3):285–288 CrossRef
- Valente JJ, Payne RW, Manning MC, Wilson WW, Henry CS (2005) Colloidal behavior of proteins: effects of the second virial coefficient on solubility, crystallization and aggregation of proteins in aqueous solution. Curr Pharm Biotechnol 6(6):427–436 CrossRef
- Vasina JA, Baneyx F (1997) Expression of aggregation-prone recombinant proteins at low temperatures: a comparative study of the Escherichia coli cspA and tac promoter systems. Protein Expres Purif 9(2):211–218 CrossRef
- Velev OD, Kaler EW, Lenhoff AM (1998) Protein interactions in solution characterized by light and neutron scattering: comparison of lysozyme and chymotrypsinogen. Biophys J75(6):2682–2697 CrossRef
- Vincentelli R, Canaan S, Campanacci V, Valencia C, Maurin D, Frassinetti F, Scappucini-Calvo L, Bourne Y, Cambillau C, Bignon C (2004) High-throughput automated refolding screening of inclusion bodies. Protein Sci 13(10):2782–2792 CrossRef
- Vonrhein C, Schmidt U, Ziegler GA, Schweiger S, Hanukoglu I, Schulz GE (1999) Chaperone-assisted expression of authentic bovine adrenodoxin reductase in Escherichia coli. FEBS Lett 443(2):167–169 CrossRef
- Willis MS, Hogan JK, Prabhakar P, Liu X, Tsai K, Wei Y, Fox T (2005) Investigation of protein refolding using a fractional factorial screen: a study of reagent effects and interactions. Protein Sci 14 (7):1818–1826
- Xu X, Jin F, Yu X, Ji S, Wang J, Cheng H, Wang C, Zhang W (2007) Expression and purification of a recombinant antibacterial peptide, cecropin, from Escherichia coli. Protein Expres Purif 53(2):293–301 CrossRef
- Yadav S, Scherer TM, Shire SJ, Kalonia DS (2011) Use of dynamic light scattering to determine second virial coefficient in a semidilute concentration regime. Anal Biochem 411(2):292–296 CrossRef
- Zhang SM, Fan R, Yang TY, Sun Y, Li JY, Xu QZ, Zhou PK (2009) An improved strategy for efficient expression and purification of soluble HIV-1 tat protein in E. coli. Virol Sin 24(6):518–528 CrossRef
- Zhou C, Qi X, Li P, Chen WN, Mouad L, Chang MW, Leong SSJ, Chan-Park MB (2010) High potency and broad-spectrum antimicrobial peptides synthesized via ring-opening polymerization of α-aminoacid-N-carboxyanhydrides. Biomacromolecules 11(1):60–67 CrossRef
- Title
- Refolding of proteins from inclusion bodies: rational design and recipes
- Journal
Applied Microbiology and Biotechnology
Volume 92, Issue 2 , pp 241-251
- Cover Date
- 2011-10-01
- 10.1007/s00253-011-3513-y
- Print ISSN
- 0175-7598
- Online ISSN
- 1432-0614
- Publisher
- Springer-Verlag
- Additional Links
- Topics
- Keywords
- Refolding
- Inclusion bodies
- Chromatography
- Static light scattering
- Dynamic light scattering
- Industry Sectors
- Authors
- Anindya Basu (1)
- Xiang Li (1)
- Susanna Su Jan Leong (1)
- Author Affiliations
- 1. School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, Singapore


text: 'The difference in the U.S. is stark, and many American companies celebrate overwork as a sign of commitment. "You have to put in the hours" is the message in the mistaken belief that the more hours you work, the more work you get done. We call this "The Cult of Overwork." Danish companies, on the other hand, recognize that employees also have a life outside of work and that working 80 hours a week is bad for both employees and the bottom line.'


text:
Life Imprisonment for Al-Qaida Operative who Targeted Financial Infrastructure
34-year-old terrorist had targeted NYSE, World Bank, IMF, Citigroup
Barot is wanted in the United States on charges of conspiracy to use weapons of mass destruction. Under British law, domestic proceedings take precedence over an extradition. He also is wanted in Yemen for the 1988 kidnappings of Westerners.
Under the alias Issa al-Britani, Barot was named in the report of the U.S. commission into the Sept. 11 attacks, as an associate of Khalid Sheikh Mohammed, the alleged planner. It said Mohammed had ordered al-Britani to identify bombing targets in New York.
The Associated Press, The British Broadcasting Corp., and Times Newspapers Ltd. successfully challenged a court ruling that threatened to prevent news media reporting details of Barot's sentencing hearing.
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page 2 - Technology Update: Aftermarket Navigation
Is your car missing a nav system? Read on...
By July 8, 2004 / Photos by Brian Blades and By Ivan Berger
Which Type?
The integrated nav and entertainment systems (about $1800 to $2700, possibly plus installation) most like factory installations are from companies like Alpine, Blaupunkt, Eclipse, Kenwood and Pioneer. To fit dashboards that aren't designed for their large (typically 6 1/2-in.) screens, their map displays retract or mount separately, allowing the systems to fit in a standard radio slot (or double-size for one Eclipse model). Using DVD drives enables them to have a street map of the whole U.S. and parts of Canada on a single disc and to play DVD movies (though only on back-seat extension screens unless the car is parked).
When turn-by-turn directions are needed, they mute the music and speak to you through the stereo speakers. Several tie in to speed sensors in the car for greater accuracy even in tunnels and other places where satellite signals don't reach. Several offer voice control. In some cars, retractable map panels may obscure dashboard features you need or activate the wiper stalk as they swing in and out.
A second type is the portable system. All cars work with portables: Just plunk one on your dash or suction-cup it to your windshield, plug it into your lighter socket, and go. Moving it among your family cars or using it in rental cars is a breeze. And the systems (from Cobra, Delphi, Emerson Mobile, Garmin, Geosat, Magellan, Navman, Sanyo, TomTom and others) sell for about $500 to $1500. Just be sure to mount them where airbags won't hurl them into the passenger compartment in an accident.
The third option is entirely PDA-based. And why beam your PDA's addresses if you can turn the device itself into a nav system? Just add mapping software, something to hold the computer as you drive, and GPS hardware that either plugs in or has a Bluetooth wireless connection. (PDAs with built-in navigation are available from Garmin, Mio and Space Machine for about $400 to $500.) Many companies make software or GPS hardware; kits with both are available from DeLorme, Pharos, CoPilot Live, TomTom and others, for about $130 to $250 for pocket computers, plus about $30 to $80 for dashboard mounts. (You'll find a wide selection, though possibly not the lowest prices, at .) Laptop computers can also be equipped this way, but mobile mounts for them are higher-priced and more intrusive.
Soon, your cellphone may be your navigator. Nextel now offers a cellphone with built-in GPS that can work with a low-cost navigation service, speaking directions and displaying turn diagrams. Other companies will have similar services soon.
Features to Look for
Browsing manufacturers' websites (which often include on-screen "demo rides") can give you some idea of what features each system has and how you might work with it. Here are some things to look for:


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End Fluoridation of Municipal Water
Water is the most essential nutrient involved in every physical function, and makes up 75% of a person's total body weight; assisting to maintain body temperature, metabolize body fat, aids in digestion, lubricates and cushions organs, transports nutrients, and flushes toxins from the body. People should drink at least sixty-four ounces per day, and if you exercise or are overweight, even more. Sources of water to re-supply the body come from the kitchen faucet, school drinking fountains, bottled water, re-constituted juices, and soft drinks. All of these sources, except homes on a well system, use treated water produced by local municipal treatment plants. The treatment process includes filtering, liming, carbonation, chlorination, and fluoridation. All are necessary to make the water potable and safe, except for the addition of Sodium Fluoride; added to change the human body and not the water itself. It is time for the US, and the few remaining fluoridating countries, to recognize that fluoridation has serious risks that far outweigh any minor benefits, violates sound medical ethics, and denies freedom of choice, and should be halted until conclusive evidence is presented to justify this practice.
Keywords: Fluoridation, controversies, endorsements, exposure, proponents, dosage, infants, freedom of choice
End Fluoridation of Municipal Water
Water in its purest form consists of two parts hydrogen bonded to one part oxygen. It covers 75% of the earth and is purified through nature's continuous cycle of evaporation, condensation, precipitation, and percolation (Pollack Laboratory, 2010). There is no more or no less water on the earth since the beginning of time; humans drink the same water the dinosaurs drank hundreds of millions of years ago. Water is the most essential nutrient involved in every physical function, and makes up 75% of a person's total body weight;...


text:
Find A Way Lyrics - The Used
Review The Song (10)
Lay your head down just for now
Space fills your mind and you dream awhile
The sun floods your room as you drown
your lungs full of breathing
Your true love believe
One more time to say I love you always
and keeping faith letting love find a way
Move let the stars suck you in
Hold tight, the night's air and breathe again
Let go and be burnt by the moon
Your hands full of feeling your true love, Believe
One more time to say I love you always
and keeping faith letting love find a way
One more time to say I love you always
and keeping faith letting love find a way
I love you to death
sponsored linksCould you love me to death (x2)
Could you love me
One more time to say I love you always
and keeping faith letting love find a way
One more time to say I love you always
and keeping faith letting love find a way
Letting love find a way (x3)
Click here to submit the Corrections of Find A Way Lyrics
Thanks to email@example.com for submitting Find A Way Lyrics.
Question | Reviewer: Allison | 12/27/10
Between the first and second verse, there's a small voice in the background. I think it said something like, "Sweetybear, you mean so much to me, and I love you very dearly." But I'm not sure if that's correct. Does anyone know exactly what it says?
Mind Reader | Reviewer: Hopeless Romantic | 12/22/07
Oh Bert McCracken! I feel it and you sing it! This song is by far the sweetest song I have ever heard. I fall in love with it more and more every time I hear it.
hey cookie cutter | Reviewer: jAy | 11/20/07
thats what it sounded like at first the voice but after a few more times listening to it i think it says "sweetie r u there, ure so prescious 2 me, u know i'll always be right there."
Beautiful song! | Reviewer: Cookie Cutter | 10/11/07
This is one of my favorite songs off the new album, which is saying somthing as they're all so good. I love the random background sounds, and bert's vocals are amaaaazing. I love the lines like "Your lungs full of breathing" and "You hands full of feeling."
There's this really quiet childish voice in the background between the first and second verse. Does anyone know what it says? It sounds like " Sweetie, are you there? Your suppoused to scream, now everybody cares..." but i'm not really sure.
almost too stunned for words | Reviewer: Jessicaa | 9/6/07
this is the single most beautiful song I have probably ever heard. I thought that all the songs on Lies for the Liars would be like Liar Liar and the Bird and the Worm when I got it, but I was totally surprised. I love the song, I love the album, I love the band, even though I've just started listening to them. It's amazing. ^^
requiem for a dream | Reviewer: me | 7/21/07
the beginning sounds in this song..and in the background..sound like the sounds from requiem for a dream (movie) when they are selling..
OMG =D | Reviewer: Daniel | 7/18/07
Certainly this is the most most beautiful of the beautifulest songs ever !!! I like the beginning, it sounds like he's taking some amphetamines =P The Used is the best band. Bert is so damn powerful <3
i love you always.... | Reviewer: Jovan | 7/9/07
oh my god
this song is amazing. bert has the most beautiful voice ever. i like the sound of the mallets in the background when he says "i love you to death"
i cry to this song. definately one of my favorites.
: ) : ) : ) | Reviewer: don't worry about it. | 6/25/07
I love The Used.!
They are basically the best band ever.
I love this song and Smother Me and Paralyzed and basically their whole new C.D., Liars For The Liars.
wow. | Reviewer: Cheyanne | 6/19/07
This song is amazing. It shows how great of a voice Bert has. I think this is my favorite song off of LFTL, next to Smother me & Wake The Dead.
The following area is only for review, if you want to submit the lyrics or the corrections of the lyrics, please click the link at the end of Find A Way Lyrics.


text:
Submitted by Publisher on Mon, 08/13/2007 - 16:14.
08/13/2007 - 15:14
Book profiles, news and previews.
- "Star Struck" A Novel by Pamela Anderson on the shelves
- 'Harry Potter and Goblet Of Fire' Voted Best Book Children Have Ever Read
- Dick York: Still Magic
- Final Stages of Printing and Distributing 10.8 Million Copies of Harry Potter and the Half-Blood Prince in Preparation for July
- Harry Potter book 7: "Harry Potter and the Deathly Hallows"
- J.K. Rowling's Seventh and Final Harry Potter Novel, Harry Potter and The Deathly Hallows, to Be Published on July 21, 2007
- Madonna launches The English Roses Website, englishrosescollection.com, based on her #1 Best-Selling Children's Book
- Movie Tie-In Book to the Film Charlie and the Chocolate Factory
- Newly Spun Science Of String Theory Now Starting To Entangle the Imaginations of Writers, Moviemakers
- The Art of Batman Begins - Book now available
- Who Is the Half-Blood Prince?
- Zagat Survey Releases 2006-2007 Guide To America's Top Golf Courses
- 'A Brother's Journey' Sequel Released - 'A Teenager's Journey'
- Bookman's Used Books and Videos on Grant, Events April 2009, Tucson, Arizona
- Borders Book Store Expands Teacher Focus for Educator Appreciation Week 2009
- Gene Simmons' Sci-Fi Comic Zipper Breaks Loose in November 2007
- Martha Stewart Living Omnimedia, Inc. to Unveil Redesigned Body + Soul Magazine
- Scholastic and Harry Potter and The Deathly Hallows Make Publishing History With 8.3 Million Copies Sold In First 24 Hours
- Zagat Releases 2007/08 Los Angeles Nightlife Survey
- Zagat Releases 29th Annual New York City Restaurants Guide, Average Meal Cost Holds Steady, Prices at High End Soar
- 'Buy the Ticket, Take the Ride: Hunter S. Thompson on Film'
- Decorating Do's and Don'ts for Home Sellers
- L. Ron Hubbard Awarded Guinness World Record as Most Translated Author
- Rick Springfield Collection' Celebrates Springfield's Return To General Hospital As Dr. Noah Drake
- Small World Toys Closes Deal on Prestigious Dr. Seuss License
- Star Jones Reynolds to Shine With Dress for Success in Seven U.S. Cities
- Stephen King's Cell: Ringing Up Readers Nationwide
- TOM PETTY Tells The Story of How the Legendary TRAVELING WILBURYS Came to Be Formed
- Zagat Releases 2006 America's Top Restaurant Survey
- Zagat Releases 2007 New York City Restaurant Survey


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Comments Update
Here's an update on our progress with comments and more.
* We've continued through this week to fix various glitches readers have reported with the new commenting system. We'll post a list here tomorrow of the different fixes we've made and which we still have to complete.
* As noted earlier, comments remain visible only to TPM Readers who are logged into the site, whether with a free commenting account or a Prime account.
* We had hoped to have comments again visible to all readers (whether or not you're logged in to comment) by the end of this week. That will not happen. We now believe we'll have our server re-architecting completed between Monday and Wednesday of next week. Once that's done, we'll redeploy comments for all readers.
* Server speed continues at normal levels. We anticipate a significant increase in download speed once our server re-architecting is complete.
* If you experience problems with the site please contact us at the email on the right.


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Investment Outlook, August 30th
- Wednesday, August 30, 2000
Written by Dr. Walter Miller, of Wright Investor's Service.
Wednesday, August 30, 2000
The case is not airtight, but most new economic data support the soft-landing scenario. Personal income has been in a slowdown for longer than the Fed has been raising interest rates, and consumers have recently been spending in conformity with their thinner wallets.
With the Federal Reserve seemingly out of the picture until after the November presidential election, last week the stock and bond markets enjoyed another strong week. Ten-year Treasury bonds rallied in the days following the Feds decision to hold the line on interest rates, hitting a yield of 5.72% on Friday. That is essentially the lowest level for the benchmark ten-year Treasury in 13 months in other words, since the Fed began raising interest rates in June 1999. Stocks did even better in the wake of Tuesdays FOMC meeting, propelling the S&P 500 Composite index to within 1% of its March high by this Monday. The S&P 400 MidCap Index reached a record high, although market breadth remains further from its 1998 peak than the MidCaps strength would suggest. The Nasdaq Composite led the market averages last week, breaking past the 4000 level for the first time in almost a month.
According to an article in Mondays Wall Street Journal, the Greenspan Fed has raised its estimate of how quickly the economy can grow without sparking inflation. The case for a higher speed limit rests on the improved outlook for productivity growth, which Greenspan believes has increased for structural which is to say, lasting rather than cyclical reasons. That labor productivity is growing at close to a 5% rate in year ten of an economic expansion is unprecedented for two reasons: first, because productivity typically exhibits its biggest gains early in a business cycle and wanes in the latter stages and second, because weve never had so long-lived an economic expansion in U.S. history. This last fact, in turn, is clearly tied up in the virtuous circle of business investment in equipment and technology and productivity growth currently being experienced.
Consumer spending rebounded in July, but not enough to controvert the fact that the U.S. economy is slowing in the third quarter. Consumer spending increased by 0.4% in real dollars last month, the best growth since February. Still, each of the five months since then has seen below-average spending growth compared to the rates of the past three years. In fact, the growth rate in consumption for the three months to July (2.6%) was the slowest such rate in three years and down sharply from the first-quarter rate (8%). Disposable personal income had already been growing at more moderate rates what might be thought of as Fed-approved, soft-landing rates for all of 2000 (since 1998, really). It is only since February that consumer spending has grown more in line with income. Spending did outstrip income in July, taking the personal saving rate to a record low 0.2%, but with consumer confidence on a plateau that sort of profligacy is unlikely to continue.
Outside of the energy sector, inflation trends remain generally benign. Price increases in what the Commerce Department calls gross domestic purchases ex food and energy, reportedly one of Alan Greenspans favorite inflation measures, have averaged only a little more than 1% over the past four months. Over the past 12 months, this core inflation rate is 1.7%, just 0.4% higher than the 35-year low of 1.3% registered from June 1997 to June 1998 amid the Asian financial crisis. Unlike the experience of the 1970s, higher crude oil prices have not spilled over into other sectors in the current environment of tough competition and productivity gains. If the U.S. economy is on course to the soft landing that the Fed has targeted, as most indicators seem to suggest, then price pressures should continue to be contained at a low level.
The investment backdrop is mostly positive for financial asset values. Economic growth moderating toward the presumed Fed target of 3.5%-4%, inflation under control, corporate earnings growth in the 10%-15% range and budget surpluses widening out in the direction of $300 billion are the foundation of better market performance. While there is not a lot of room in stock P/Es for major disappointment, so long as the U.S. economy remains in its virtuous productivity cycle, higher-than-normal valuations are appropriate and positive stock returns likely. Dont expect stock returns of the 20%-25% magnitude seen in the late-1998 to early-2000 period, but rather returns more in keeping with the markets long-term performance and more than competitive with bond returns.
Investment Outlook
After the gains of recent weeks, some profit taking should probably be expected in the bond market in the near term. We would view such a break as an opportunity to extend the duration of bond portfolio holdings modestly beyond the benchmark duration. Stocks are exhibiting better trends than they have in months both more persistence to the upside and more rationality in market action. By the latter we mean stock prices moving for fundamental reasons as opposed to the sometimes speculative, sometimes frivolous market action of late last year and early this year. This is healthier for stocks and more supportive of a lasting extension of the bull market.
For additional investment analysis, visit Wright Investor's Service.
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