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Summary: Alpha mannosidase, middle domain
Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.
This is the Wikipedia entry entitled "Glycoside hydrolase family 38". More...
Glycoside hydrolase family 38 Edit Wikipedia article
golgi alpha-mannosidase ii | |||||||||
Identifiers | |||||||||
Symbol | Glyco_hydro_38 | ||||||||
Pfam | PF01074 | ||||||||
Pfam clan | CL0158 | ||||||||
InterPro | IPR000602 | ||||||||
SCOP | 1o7d | ||||||||
SUPERFAMILY | 1o7d | ||||||||
CAZy | GH38 | ||||||||
golgi alpha-mannosidase ii | |||||||||
Identifiers | |||||||||
Symbol | Alpha-mann_mid | ||||||||
Pfam | PF09261 | ||||||||
InterPro | IPR015341 | ||||||||
SCOP | 1o7d | ||||||||
SUPERFAMILY | 1o7d | ||||||||
golgi alpha-mannosidase ii | |||||||||
Identifiers | |||||||||
Symbol | Glyco_hydro_38C | ||||||||
Pfam | PF07748 | ||||||||
Pfam clan | CL0103 | ||||||||
InterPro | IPR011682 | ||||||||
SCOP | 1o7d | ||||||||
SUPERFAMILY | 1o7d | ||||||||
CAZy | GH38 | ||||||||
In molecular biology, glycoside hydrolase family 38 is a family of glycoside hydrolases.
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy(http://www.cazy.org/GH1.html) web site,[4] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes. [5]
Glycoside hydrolase family 38 CAZY GH_38 comprises enzymes with only one known activity; alpha-mannosidase (EC 22.214.171.124) (EC 22.214.171.124).
Lysosomal alpha-mannosidase is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover. The enzyme catalyzes the hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides, and can cleave all known types of alpha-mannosidic linkages. Defects in the gene cause lysosomal alpha-mannosidosis (AM), a lysosomal storage disease characterised by the accumulation of unbranched oligo-saccharide chains.
A domain, which is found in the central region adopts a structure consisting of three alpha helices, in an immunoglobulin/albumin-binding domain-like fold. The domain is predominantly found in the enzyme alpha-mannosidase.[6]
[edit] References
- ^ Henrissat B, Callebaut I, Mornon JP, Fabrega S, Lehn P, Davies G (1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proc. Natl. Acad. Sci. U.S.A. 92 (15): 7090–7094. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=41477.
- ^ Henrissat B, Davies G (1995). "Structures and mechanisms of glycosyl hydrolases". Structure 3 (9): 853–859. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ^ Bairoch, A. "Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT". 1999.
- ^ Henrissat, B. and Coutinho P.M. "Carbohydrate-Active Enzymes server". 1999.
- ^ CAZypedia, an online encyclopedia of carbohydrate-active enzymes.
- ^ Hough E, McSweeney S, Schoehn G, Heikinheimo P, Helland R, Leiros HK, Leiros I, Karlsen S, Evjen G, Ravelli R, Ruigrok R, Tollersrud OK (2003). "The structure of bovine lysosomal alpha-mannosidase suggests a novel mechanism for low-pH activation". J. Mol. Biol. 327 (3): 631–44. doi:10.1016/S0022-2836(03)00172-4. PMID 12634058.
This article incorporates text from the public domain Pfam and InterPro IPR000602
This article incorporates text from the public domain Pfam and InterPro IPR011682
This article incorporates text from the public domain Pfam and InterPro IPR015341
This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.
This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.
Alpha mannosidase, middle domain Provide feedback
Members of this family adopt a structure consisting of three alpha helices, in an immunoglobulin/albumin-binding domain-like fold. They are predominantly found in the enzyme alpha-mannosidase [1].
Literature references
Heikinheimo P, Helland R, Leiros HK, Leiros I, Karlsen S, Evjen G, Ravelli R, Schoehn G, Ruigrok R, Tollersrud OK, McSweeney S, Hough E; , J Mol Biol. 2003;327:631-644.: The structure of bovine lysosomal alpha-mannosidase suggests a novel mechanism for low-pH activation. PUBMED:12634058 EPMC:12634058
External database links
Pseudofam: | PF09261 |
SCOP: | 1o7d |
SYSTERS: | Alpha-mann_mid |
This tab holds annotation information from the InterPro database.
InterPro entry IPR015341
Members of this entry belong to the glycosyl hydrolase family 38, This domain, which is found in the central region adopts a structure consisting of three alpha helices, in an immunoglobulin/albumin-binding domain-like fold. The domain is predominantly found in the enzyme alpha-mannosidase [PUBMED:12634058].
Gene Ontology
The mapping between Pfam and Gene Ontology is provided by InterPro. If you use this data please cite InterPro.
Molecular function | hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0004553) |
zinc ion binding (GO:0008270) |
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
Loading domain graphics...
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...
View options
We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (126) |
Full (2561) |
Representative proteomes | NCBI (2123) |
Meta (87) |
Jalview | ||||||||
PP/heatmap | 1 | |||||||
Pfam viewer |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key: available, not generated, — not available.
Format an alignment
Download options
We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
Seed (126) |
Full (2561) |
Representative proteomes | NCBI (2123) |
Meta (87) |
Raw Stockholm | ||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
External links
MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Seed source: | pdb_1o7d |
Previous IDs: | none |
Type: | Domain |
Author: | Sammut SJ |
Number in seed: | 126 |
Number in full: | 2561 |
Average length of the domain: | 78.00 aa |
Average identity of full alignment: | 28 % |
Average coverage of the sequence by the domain: | 8.09 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details: |
Model length: | 80 | ||||||||||||
Family (HMM) version: | 6 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
Sunburst controls
ShowThis visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...
Tree controls
HideThe tree shows the occurrence of this domain across different species. More...
Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Alpha-mann_mid domain has been found. There are 64 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
Loading structure mapping...


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Anthony, that is anecdotal. Did you notice where I added the qualifying adjective "most"? Please forgive me, but this one DID almost make me fall out of my chair from laughing so hard: "I'm Catholic. I bet you didn't know that." Is that a pick-up line...? REALLY? "Anthony, Church is a chore to me, not a "comfort"" -That's fine...but people who have tried to convince me to go to church in the past have told me what a comfort it can be for them, and I'm sure it is. I imagine that church/mass/temple can be many things to many different people. It's not always easy, though, to roll out of bed early on any given Sunday, get ready, and have to go out regardless of the weather. My mom and stepdad found a way around that, though, and watch Lon Solomon's service at McClain (sp?) Bible Church from the comfort of their home - and their PJ's and coffee - via the internet. ":-) Perhaps I am rising to the bait, Anthony, " ~:-) to you as well, Kevin Peet. Sorry to say though, there is no bait, it was just a question for Megan I had out of slight confusion. It was not intended to start debate or insult anyone - just simply my observations from past experiences and wanted to get a better understanding of what she was talking about. And she did, thank you Megan :) "May I ask how you are using the word 'tolerate'?" ~Sorry Kevin P, I was referring to #2 of the Merriam-Webster definition of "tolerance" when I initially posted that. I should have specified. http://www.merriam-webster.com/dictionary/tolerance and # 2 of http://www.merriam-webster.com/dictionary/tolerance "Er...I am not sure that "quite lovely" is the compliment most boys desire." -Sorry Jason, but I respectfully disagree :) One night a couple of years ago I went dancing at one of our local dance clubs, and some random very attractive girl came up to me while I was talking to some friends and blurted out, "Aww! You're SOOOO pretty!" then handed me her phone number on a napkin and gave me a kiss on the cheek and left. (For the record, she didn't appear to be intoxicated. Bonus.) I never used her phone number, but that compliment is still etched in my memory. I think lovely is a great word to use! "I think Christians have some stuff to learn about other religions - not to say that we agree with their worldviews, particularly, but just to clear up some "misjudgments."" -This is my favorite part of your response, Megan. I've taken some time to study other religions, and I've learned so much that I never knew before. Maybe this could be more than just about clearing up misjudgements. This could also be a fascinating learning experience, as well...? Er...I am not sure that "quite lovely" is the compliment most boys desire. There are few so obnoxious as a new convert with only a little discipleship under their belt, so I'm not going to go all preachy on you, Megan, with the news that ellipses have spaces before the dots: " . . .". (Gina will know what this means. If you'd prefer to remain baffled, well, I understand.) But I will say that the Vishal Mangalwadi book should make for an interesting discussion at an IFYC meeting . . . Anthony, Interfaith is talking about ALL faiths, not just Christianity. In fact, that's why I support it so much because I think Christians have some stuff to learn about other religions - not to say that we agree with their worldviews, particularly, but just to clear up some "misjudgments." Also, don't worry… I don't have anything against boys… the ones in the elevator just got a little "too" carried away. A lot of boys are quite lovely. :-) Here I go again :-) Perhaps I am rising to the bait, Anthony, but you write: "I ask because of all the different faiths/religions in the world that are practiced, and the many interpretations of Christianity that have been made over the past several years. It's always been my view that more conservative-leaning Christians cannot/do not/will not tolerate other beliefs/belief systems." May I ask how you are using the word 'tolerate'? I ask because these days it has come to have two informal meanings. In one instance, it means to permit another person to believe what he or she wishes, without any recriminations or refusal to accept the person. In the second, more slippery (but I think more common) use, it means to refuse to take a stance on the truth-content of WHAT they say, on the fuzzy ground that 'who is to say what is true or not?' or something along those lines. I find the second use to be really weak reasoning, and generally the result of intellectual gutlessness, the refusal to take a stand-- based, most likely, on not having any rational basis for one's beliefs. Here's a simple example: if the Bible says there is just ONE God, while Hinduism says there are 5 million gods, doesn't simple reason dictate that both statements cannot both be true? Either one or the other must be incorrrect (unless we're using language in a very slippery, shape-shifting manner), or possibly both are incorrect. I don't see that tolerance has the slightest thing to do with this assertion. Here is one core of the distinction: one view looks at the content of what is said and evaluates that, the other looks at the person saying the statement, and does not make a distinction between what is said and the person saying it. I know it has become (oddly enough) a radical thing to maintain, but the truth of a statement is completely independent of the person making the statement. And therefore, good people can believe something that is false, while evil people can believe what is true. And finally, another sub-set: the second kind of 'tolerance' may be based upon the view that, "well, in the spiritual/metaphysical realm, who can possibly know what is true??" I will contend that in most cases, this is more likely revealing that person's lack of intellectual rigor in pursuing the matter. But in addition, in a way it's a self-defeating statement: to say that one can know nothing about the metaphysical world is simultaneously claim that one knows something about the metaphysical world, that it is 'unknowable.' To say you know something which cannot be known is self-contradictory. But I think that usually the speaker is more likely admitting, "*I* myself know nothing about that world, and therefore I am extrapolating and maintaining that no one can know anything about it." And THAT is a strong statement, worth investigating and either proving or refuting. Because if that world can be known, even if imperfectly, then it is very possible that the claims of that metaphysical world are completely and utterly compelling on all human beings. Anthony, Church is a chore to me, not a "comfort". PS to Megan: On a scale of 1-10, this post gets a 9.5, because although I'm not a church-goer (and should not be chastised because of this), I respect that people worship in many ways and if church/temple/mass is a way and a comfort for them, they should go for it and should NOT be condemned. Good for you for sticking to your guns. "Thankfully, however, a Chicago-based organization called Interfaith Youth Core (IFYC) has decided to challenge these notions by bringing "college students together across faith and belief lines so that they develop greater respect, comfort and appreciation for one another and their traditions."" -Megan, I have a Question: Are you referring to only Christianity when you state "bringing college students together across faith and belief lines"? I ask because of all the different faiths/religions in the world that are practiced, and the many interpretations of Christianity that have been made over the past several years. It's always been my view that more conservative-leaning Christians cannot/do not/will not tolerate other beliefs/belief systems. I do not mean that in a harsh or insulting way, but I've attended services at conservative-leaning churches, was educated in (STRICT) Catholic school from ages 4-12, and that seems to be the general sentiment I've noticed. And what do you have against us BOYS young lady?? ;) There are a (large) number of us out there who give hugs and affection openly, support women's rights, and not all of us are out to "get laid". Don't put all of us on the same boat, Megan, not all of us are sleazy, slimy dirtballs! :) "I have no doubt, Jason, that a whole host of people read those words and immediately offered heartfelt praise that indeed, there could have been two of me, but God in His mercy stayed his hand." -No matter what I read or wherever I go in my explorations of BreakPoint's blog, I know I can always count on some post of LeeQuod's to either make me smile or have me in stitches. Good one :) Except the main reason for guilt is the(justifiable if not conclusive) belief that Christianity specifically forbids the use of arms in it's defense. There is no logical reason from Natural Law. That is while anyone can be expected to get, "thou shalt not steal" or "honor thy father and mother" no matter where and when he lives, there is no reason except the tenants of a given faith why a person should not fight for his religion. That is, the proposition "It is permissible to use force in self-defense and/or the defense of others and to cooperate for such" is generally agreed upon even if how far that extends is not. That being admitted the focus of collective self-defense can be a tribe, a city, or a nation, but there is no logical reason why it should not be a religion. For instance, the Sikh practice of carrying plastic swords as a symbol of their status as a warrior faith is immune to criticism from outsiders; because Sikhism is a warrior faith. If a Jain were to do so, however he would be obviously inconsistent. Likewise while rude, it is perfectly logical to answer someone who criticizes the Crusades simply with "Who made you Pope?" That is the literal answer. The speaker is often neither himself a Christian nor well-studied in Christianity enough to give an actual answer. And only someone who is one or the other of these can actually say that the Crusades were a discredit to Christianity because otherwise they are not competent to give an opinion. An outsider has no way to say, "The Crusades were immoral" if it could reasonably be said that Crusading would have been defensible if conducted in the name of another institution. For instance the Byzantines were an ally of the Crusaders but though Christian, Byzantine soldiers fought for the Emperor. And no one says "the Byzantine Empire was immoral to fight in the Crusades." Stark's book "God's Battalions" is very good on the subject of the Crusades. And I love J. Budziszewski's columns. The basic reason people want to say stupid and cruel things about Christians is the guilt they know they have. Actually, I had less of a problem that way then many, being a historian, not to mention a Victorian fan from time immemorial. When you have been raised on Ballad of East and West you are not likely to think all Moslems are demons but neither are you likely to think they are adorable little adherents to a Religion of Peace. LeeQuod, How times have changed. Dorms do have co-ed floors now, where guys and girl live on the same floor together. It's quite interesting :) I learned to stay away from the boys pretty quickly. haha. "Better Together," I think, does focus on people who already have faith, but I got the feeling that it also reaches out to those who don't. I'll research it a little more for clarity. Although, I do think there is a need for education even with people who do have faith. I think some (but not all, of course) Christians get the wrong impression of Muslims (thinking they are all violent or terrorists) and, as I mentioned, many people get the wrong impression of Christians. A lot of that has to do with the media, in my opinion. I value organizations that seek to integrate all religions and tolerate all people of faith, whether or not we agree with their respective faiths. Kim, I haven't read "The Real History of the Crusades," but I've read some articles about what really happened, probably with ideas similar to those in the book. I was just trying to illustrate what are some of the prevailing notions at school about Christians in general. The only male moslems I am aware of that wear veils are Touregs. Interestingly not all female ones traditionally did(Charles M Doughty once said that he liked the tribes wear the women wear no veil best and that was not flattering his hosts; he was not much for that). I could imagine myself as a Jew going to Synagogue, eating Kosher, arguing Torah, and killing terrorists. As for Buddhists, most people seem to connect Buddhists with romantic thoughts of mysterious Himalayan temples with Tomes Of Ancient Lore, and secrets Man Was Not Meant To Know. Or of stoic samurai, tea ceremonies and of course with gorgeous Wuxia princesses who are of course martial arts experts. One problem is that Evangelicalism isn't exotic or elegant and even when it tries to be doesn't have the knack for it; you have to have several hundred years of backlog to do that sort of thing properly and Evangelicalism is a middle class suburban religion. In a way it is the same aesthetic inferiority complex that Americans have vis-a-vis Europe. As for the Crusades, going to far to defend them is odd though they were no worse then any war of the time. One thing to point out is that unless you are starting from a particular doctrine it is hard to say that it is more immoral to fight for one's religion then for any other form of local identity so the phrase "religion causes wars" is logically flawed to anyone but a pacifist. As far as getting along with people from other religions, I find them more pleasant company then materialists. The chief advantage I will give materialists is that they are very much aware that they live in an unjust universe and in some ways rage is better then complacency(in other ways complacency is better; it causes less trouble). I do believe however that materialism is logically contradictory(the phrase "there is only matter" is a philosophical proposition not a scientific one), and does not properly account for the complexities of the human mind without convoluted myths that are interesting as myths but are obviously myths(I still haven't heard why lions are beautiful and hogs are ugly even though lions are competitors and hogs are breakfast). Ah, Gina, so many excellent articles, so little time. But Megan and "Prof Theo" make an incredibly powerful combination, so I'm delighted to see it's occurred. Lee, funny you should mention J. Budziszewski to Megan . . . :-) http://www.breakpoint.org/features-columns/articles/entry/12/19583 "though I am not LeeQuod" I have no doubt, Jason, that a whole host of people read those words and immediately offered heartfelt praise that indeed, there could have been two of me, but God in His mercy stayed his hand. And indeed, when you name a religion "Submission", it's a safe bet that its most important value is submission. And that mercy operates on a sliding scale, weighted toward those who are more fully submitted. Megan, firstly I'm very sorry that you had to suffer the boors in the elevator every week. ("the boys on my floor"?? In my day "co-ed dorm" meant boys on one floor and girls on another. Ick.) John 15:20-21 springs to mind, as does Acts 5:41. Secondly, though, I must admit I'm a bit puzzled: Doesn't "Better Together" aim to connect those who *already* have faith? Versus, say, those who are going out seeking alcohol and sex? I mean, I understand that the result would be a greater interfaith dialogue and understanding, but the lack of those doesn't seem to be the cause of this rude behavior. So I'm not making the connection of how their treatment of you would improve if only they understood your faith. And I'm not trying to start something with Jason or anyone else, but I doubt you'd get the same treatment if you were going out in a hijab, or if you said you were going to the synagogue or even a Buddhist meditation center. No, you get picked on because it's culturally acceptable for them to so. I think this is less a problem of ignorance and more a problem of tolerance of inappropriate behavior. Educating them will simply make them smarter jerks. No doubt you already know about the works of J. Budziszewski. I wonder if anyone at PFM knows him personally, and could arrange an introduction for you (if one's really needed); a pity that Mizzou and UT Austin are so many miles apart. And there's some guy named Eric Something-or-other at PFM ;-) who has a series of absolutely awesome books titled "Everything You Always Wanted to Know About God (but were afraid to ask)" that would be excellent to give to someone who's actually open to learning about your faith. I highly recommend "The Jesus Edition" of the series. And maybe an elevator ride's just long enough to ask them, politely but with conviction, where they got their misconceptions. Eric has an incredibly disarming way of doing this in his books and his speeches; it's well worth studying. Oh, and Kim's right about the Crusades; the common understanding of them is almost exactly the opposite of the truth. Rodney Stark has written several very accessible volumes on the topic. And I, too, thank God there aren't two of me, because if there were, this comment would be twice as long. Nice article. But though I am not LeeQuod, even I don't believe that the most important value in Islam is mercy.


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Description Report Fraud
Oldsmobile 442 Convertible Pace Car (Clone), White, for sale in San Luis Obispo, California, for $21,900.
Features. .
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Exterior. .
Excellent restoration for Olds Corp. show/celebration in Lansing, exact replica of Indy 500 Pacecar.
Interior. .
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All restored as new/rebuilt, less than 8,000 miles on vehicle since restoration in 1997. Original "base"Cutlass 350/350 replaced with 1970 Olds 442 drivetrain: 455 cu. in. motor and TH 400 trans. BRAND NEW rebuilt Harrison radiator! The AC works fine.
Notes. .
Great "Show"car, but "EXCELLENT DRIVER"too, real Muscle Power and great investment! 2nd owner - owned since 1978. Bought from original owner in Las Vegas. VIN: 342670E168250 Data Plate or Cowl Tag Info. : ST 70 34267 B 2900 04A 997 78. Work performed in 2010/2011: 1) Front axle was converted to disc brakes. This give the car much better stopping power which is necessary with the high HP 2) New shock absorbers 3) All fluids changed 4) Battery system was rebuilt. Converted to a 6 cell/12 volt high performance system with a high output alternator
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Transmission:Turbo 400 automatic
Base Colour:White
Location:San Luis Obispo, California


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Feb 28, 2013, 02:52 PM | #1 | |
Apple Has Sold 8 Million iPads to Worldwide Educational Institutions
Today, Apple revealed to TechCrunch that the company has sold more than 8 million iPads to educational institutions worldwide, accounting for some 6.6% of total iPads sold, with 4.5 million of those to U.S. based schools. In a number of Apple's quarterly earnings calls over the past couple of years, Apple executives have repeatedly shared details of large transactions to educational institutions and of an iPad adoption rate by educational institutions that was unlike any Cook had seen before. In the Q3 2012 conference call, the company noted that a school district in Texas purchased 11,000 iPads, and then went on to talk about the recent price drop of the iPad 2 to $399: Quote:
Apple today said that 1 billion pieces of free content have been downloaded from its iTunes U service since its inception. Article Link: Apple Has Sold 8 Million iPads to Worldwide Educational Institutions |
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Feb 28, 2013, 03:01 PM | #2 |
I'm not surprised. The iPad is relatively affordable and it is Apple's most durable computing product. It is hard to break one.
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Feb 28, 2013, 03:07 PM | #3 |
yet the school i graduated from 10 years ago still uses books from 1997
'13 MacBook Pro Retina iPhone 5 (white & silver) Nexus 4 iPad 3 Kindle KindleFire HD Apple TV 3 |
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Feb 28, 2013, 03:20 PM | #4 |
I wish we had iPads in school, I would've paid so much more attention.
On a second thought, probably not that much more. |
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Feb 28, 2013, 03:28 PM | #5 |
If used right, it can be a very powerful tool. My daughter is using an iPad in school and got from the reading tutor software to improve her reading skills - it really improved her reading and the fun in doing it by a lot. It all depends in using the right software (there is unfortunately too much bad software from people trying to make a quick dollar - but there is good one too)
Also starting next school year they do a pilot program where they start replacing physical text books with corresponding iPad editions. They still will have the physical book in school (at least initially), but they no longer have to carry tons of books forth and back every time and it will eliminate the problem that they 'forget' the book needed for the homework (I know, that's my daughters fault, but still, it helps). If the pilot is successful, they might go ahead and no longer use the physical text book - but depends on how all of that is working out. Advantages will be: - more fun (hopefully) - quicker update cycles than 'printed' text books - more interactive content - cheaper in the long run
sent from my computer |
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Feb 28, 2013, 03:32 PM | #6 |
AND yet not one of those iPads was reduced in price with an educational discount. Way to go apple, you greedy bastards.
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Feb 28, 2013, 03:43 PM | #7 |
Remember that many teachers would be extremely happy if you donate your old iPad to a school when you get the new-new iPad...
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Feb 28, 2013, 03:44 PM | #8 |
So apple has sold more iPads to educational institutions alone, than the amount of tablets most other companies sold to the entire world
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Feb 28, 2013, 03:46 PM | #9 |
My university purchased a lage batch of new ipads a month ago- top spec ones with GPS and 3G for our undergraduates. I was about to issue them to my students.....only to be told by our IT people that they had all been stolen from a 'secure' area. We still have our old Mobile Windows PDA devices that nobody in his right mind would want to steal.
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Feb 28, 2013, 03:52 PM | #10 |
School districts are buying thousands of iPads using bond money, which is being financed over 30 to 50 years in some cases. Tax payers will end up paying $4000 + for iPads that will last...., well you tell me how long an iPad in the hands of children are good for?
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Feb 28, 2013, 06:31 PM | #11 | |||
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LW Apps iPhone iOS Applications LW Apps Twitter Best iPhone Apps: Studious Diary/Planner: bit.ly/PqTzyw Are You Normal?: m.lwapps.com/15HN0it Phys Pad: bit.ly/Pj86Oa |
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Feb 28, 2013, 09:21 PM | #12 |
Our district got a discount on a volume purchase. Wasn't much of a discount, but then again our purchase was small compared to others. (15 to 20 I think)
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Mar 1, 2013, 01:50 AM | #13 |
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Mar 1, 2013, 01:55 PM | #14 |
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Reporting Real Life, Telling True Stories
Screening of |
True/False Film Fest |
wT/F - True False Zine |
The rise of documentary film as a social and box office force continues unabated, even as traditional journalism struggles to find its financial footing in an era of new, and often free, sources of information. Sicko (2007), Michael Moore's exploration of the American healthcare system, grossed $25 million – enough to pay a year's salary (and health insurance) for a couple hundred investigative reporters at the newspapers that can no longer afford them. Oscar-winning films like Inside Job (2010) and The Cove (2009) are simultaneously entertaining audiences and investigating serious issues like the financial collapse and dolphin hunting – issues that in the past might have been covered exclusively by investigative journalists.
That a mainstream journalism outlet like CNN has just created a documentary film unit harkens back to an era when documentary was mainstream, the province of television networks intent on fulfilling public service requirements and bolstering their images. What explains the public's fascination with documentary? How is documentary film reacting to recent transformations in the media landscape? Is it filling a critical need that journalism is no longer willing or able to meet?
For all their historical and institutional differences, documentary film and journalism intersect in their commitment to depicting real life. Join us in Columbia, Missouri, February 27–28 as a dynamic group of scholars, filmmakers and film critics engage in a series of thoughtful and energetic discussions about these intersections of documentary film and journalism and the implications for America's media of the future. The second year of Based on a True Story: The Intersection of Documentary Film and Journalism will analyze further the intersection of nonfiction storytelling forms, advocacy in that storytelling, and the cultural and ethical implications of the convergence between journalism and documentary film.
An interdisciplinary group of scholars at the University of Missouri — sponsored by the Mizzou Advantage Program — has partnered again with the True/False Film Festival to continue a tradition of light-hearted yet serious-minded discussions about documentary film. Conference attendees will spend time engaging with some of the top thinkers, purveyors, and practitioners of documentary film. (The perfect warm-up to the tenth annual True/False Film Festival, February 28 – March 3, 2013!)
The conference begins on Wednesday evening, February 27, with a screening and Q&A with Academy Award nominated filmmaker Kirby Dick. On Thursday, the discussion continues with four panels scheduled throughout the day, along with refreshment and lunch breaks to provide ample opportunity for interaction with the conference participants and attendees.
We hope you'll join us for the conference and choose to stay on in Columbia for the True/False Film Festival, considered by many industry insiders as the best national documentary film festival.


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"All war is deception."
Author details
Other Sun Tzu Quotes
For to win one hundred victories in one hundred battles is not the acme of skill. To subdue the enemy without fighting is the acme of skill.
Sun Tzu
83% of people like this quote
Supreme excellence consists in breaking the enemy's resistance without fighting.
Sun Tzu
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What can I do to configure SSH on both client and servers to prevent Write Failed: broken pipe
errors, which ofter occurs if you sleep your client computer and resume later.
I have tried this in
This is how often, in seconds, it should send a keepalive message to the server. If that doesn't work then train a monkey to press enter every two minutes while you work. | |||||||||||||||||||||
SSH sessions may break due to numerous and possibly unavoidable reasons. A useful utility which can be used to mitigate problems caused by this is called | |||||
I'm remotely upgrading an Ubuntu server from lucid to precise and lost the ssh connection in the middle of the upgrade with the message "Write failed. Brocken pipe". ClientAliveInterval and ServerAliveInterval did nothing. The solution is to turn on TCPKeepAlive options in client ssh:
| ||||
I have a script on the remote server that never seems to fails, regardless of the SSH configuration client or server.
Save it to some dummy.sh file and quickly run it before you minimize the window or move away from it. It will keep printing the current time stamp on the server and keeps your connection alive as long as the connection is not dropped by any other reason. When you get back to that terminal, just hit CTRL+C and keep working. | |||


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Select a datex
This four-hour city sightseeing tour of Oslo is a fabulous introduction to the city and a convenient way to see the major attractions. You'll visit Vigeland Sculpture Park, see the Kon-Tiki Museum, stop by the Holmenkollen Ski Jump and explore downtown Oslo.
As the capital of Norway, Oslo is the country's hub of culture and tourism. The city is beautifully situated at the head of the Oslo Fjord (Oslofjorden in Norwegian) and is surrounded by forested hills. In other words, it's quite stunning, and it offers several museums and other attractions.
You'll tour the city by coach, stopping at the Vigeland Sculpture Park and some of the city's many museums, including the Fram Museum (home to the Fram, a famous vessel used in Norwegian polar expeditions) and the Viking Ship Museum (home to three preserved ships used by Vikings in the 9th and 10th centuries).
You'll also visit the Kon-Tiki Museum to see the Kon-Tiki, the famous raft from the 1947 Norwegian expedition across the Pacific Ocean, and you'll stop by the Holmenkollen Ski Jump.
Schedule details
Vigeland Sculpture Park
Select a month to view a calendar of availability and departure details.
Departure Point:
Tour departs from Radhusbrygge (pier 3) opposite Oslo City Hall. Between September and May tour departs in front of City Hall.Departure Time:
- 10:15am
Return Details:
Returns to original departure pointPricing information
Click the link below to check pricing & availability on your preferred travel date. Our pricing is constantly updated to ensure you always receive the lowest price possible.
Additional information
Oslo City Sightseeing
- Coach tour
- Entrance fees to Museums
- Local guide
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- Food and drink, unless specified
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February 3, 2013
Ryan James GopherIllustrated.com
Minnesota outlasts Iowa 62-59 as Austin Hollins hits a three and creates a turnover in the final 13 seconds for the win. ...More... To continue reading this article you must be a member. Sign Up Now for a FREE Trial
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Hi. Is there a site that lists rather generic storylines with plot devices (rescue the princess, deliver the orb etc.) for playing with action figures motu or otherwise? (for kids)
How do you like that, hero!
See ya',
He-BroI have a custom Nikolas Powers and many figures for sale on eBay, some are even the good ones too.
My Shapeways Store SALE- some cool custom accessories like the Diamond Ray of disappearance, TrapJaw's Attachments, Coridite Crystals, Teleportation Device, Stasis Ray, Horn of Evil, Tytus Hammer, etc...


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Michael Saunders & Company is pleased to welcome Brian McColgan to the Boca Grande office.
A longtime real estate professional, Brian, who holds the MAI designation, brings an extensive commercial background specializing in appraisal review, litigation counseling and expert witness testimony, as well as conducting hearings as a Special Magistrate on commercial real estate and high-end residential waterfront properties.
After founding McColgan & Company, Inc. in 1980, Brian expanded his real estate appraisal firm to become the largest privately owned, commercial-only appraisal company in the southeast United States. During this term, he managed and operated offices in Atlanta as well as the Tampa and Fort Myers market areas. The role of his company included assignments in approximately 30 states and encompassed
almost every aspect of commercial real estate. Brian sold his business in late 2006, and continues to provide appraisal review and counseling services for the company's Florida assignments.
Raised in an army family, Brian graduated from high school at Calvert Hall College in Towson, Maryland, before earning a bachelor's degree from the University of Miami. Brian discovered and made his home on Useppa Island in 1985. From the Boca Grande office, he specializes in Useppa residential properties.


text: Since the time when Talcott Parsons pursued the project of one overarching "general theory of society", the landscape of social theory has vastly changed, and the pluralism and multidimensionality increased tremendously. Today, with so many different approaches in and to social theory, and multiple ways of defining and describing their relationship to and relevance for the social sciences, there has been a growing danger of diversity and pluralism tipping into fragmentation, making the prospect of social scientists and sociologists being able to communicate with the expectation of reaching some kind of understanding, ever less likely. This volume presents alternative trajectories for how to take steps toward achieving a theoretically informed understanding of the present analytical and practical challenges (in terms of social, sociological, and critical theory), and looks beyond pluralism and fragmentation to the kind of roles social theorists may be playing in the future. These essays revisit the issue of common agenda (or lack thereof) in social theory and provide critical overviews by specialists working in social theory, sociological theory, and critical theory.


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Entrapment [DVD]
Screenplay : Ronald Bass and William Broyles Jr. (story by Bass and Michael Hertzberg)
MPAA Rating : PG-13
Year of Release : 1999
Stars : Sean Connery (Robert MacDougal), Catherine Zeta-Jones (Gin Baker), Ving Rhames (Thibadeaux), Will Patton (Cruz), Maury Chaykin (Conrad Greene), Kevin McNally (Haas), Terry O'Neill (Quinn), Madhav Sharma (Security Chief), David Yip (Chief of Police)
"Entrapment" is a technically efficient thriller with no human interest or development. Despite the fact that the leads are played by the always charismatic Sean Connery and Catherine Zeta-Jones, the smart and sexy siren who lit up the screens last summer in "Mask of Zorro," "Entrapment's" characters never rise above simple, comic book creations.
The film is about high-stakes international thieves, the kind who think stealing the Crown Jewels is too easy. Connery plays Robert "Mac" MacDougal, a veteran thief of great notoriety who teams up with Zeta-Jones' Virginia "Gin" Baker, an insurance investigator who may or may not be an experienced thief herself. This part of the story works—screenwriters Ronald Bass and William Broyles Jr. and director Jon Amiel ("Copycat") put together three major robberies during the film's two hours, each one upping the ante in danger and reward.
However, "Entrapment" is yet another film that asks us to buy a romance between a 69-year-old man and a 29-year-old woman. And, even though that 69-year-old man is Sean Connery (give him credit for still moving like he's 30 years younger and looking a helluva lot better than Clint Eastwood), he is still unable to generate the kind of chemistry with Zeta-Jones that is required for us to accept this age gap romance.
Interestingly enough, the movie is able to work up some heat, but it is always in suggesting a potential relationship, not in actually following through on it. For instance, director Amiel slyly turns one of Gin's training exercises—she practices slinking and crawling through strands of taught yarn to perfect her moves so she can avoid a laser beam security system—into an erotic dance of seduction. Using heavy breathing on the soundtrack and slow motion fades between Zeta-Jones' cat-like ballet moves and Connery's voyeuristic gaze (she has her eyes closed, but you can feel that she feels him watching), Amiel gives the scene an effective erotic heat, much more so than most clumsy sex scenes you find in movies today.
"Entrapment" would have been significantly better if it had maintained this coy distance between them, allowing for heat without actual friction. The distance gives the relationship an exotic, forbidden feel which, in the real world, it probably should have. Only in Hollywood are romances between men and women with 40- to 50-year age differences considered a given. Too bad the filmmakers were unable to keep themselves from ending the movie in ridiculously romantic fashion—it makes you wish for the heyday of the 1970s when an action movie like this would have ended on some self-conscious ironic or existential note.
However, before the movie arrives at its lame conclusion, it does feature a number of tense, well-directed action sequences involving complicated robberies of a $40-million Rembrandt painting, a 2,000-year-old Chinese mask, and $8 billion from an international trading bank in Malaysia. Amiel mixes up the action—part of the time it's "Mission: Impossible"-like suspense involving alarm systems and laser beams, while other times it's James Bond-style action with Connery and Zeta-Jones dangling hundreds of feet in the air from a slowly breaking string of light bulbs.
Each robbery is intricately plotted out, and what fun the movie has to offer is watching Connery and Zeta-Jones execute the plans. Amiel lets us watch them working out what they're going to do, and their preparation never completely makes sense until we actually see the robbery—it gives you something to look forward to. Each plan is a little more complicated than the one before; and, of course, the stakes are always higher, both in terms of getting caught and making off with the loot.
Unfortunately, this puts a spotlight on the movie's major flaw, namely the fact that it never manages to rise above a certain level because the stakes are never more meaningful than getting caught or getting rich. Neither Mac nor Gin have any background or color; they're simply thieves who happen to look as good as they are at stealing. This is especially problematic since the movie is asking for us to root for unrepentant criminals who are only out for themselves.
There's also no reason why these two should fall in love, unless it's simply because they realize they're so perfect for each other professionally—once again, they are defined only by what they do, not by who they are. If the screenplay had given them some kind of need to fulfill beyond their own egocentric desire to pull off the world's greatest robbery, then maybe "Entrapment" would have had more kick. Instead, it's simply a clever, one-trick thriller that's watchable, but not memorable.
"Entrapment" has been re-released by 20th Century Fox in a new Special Edition.
Widescreen: 2.35:1
16x9 Enhanced: Yes
Audio: Dolby Digital 5.1; Dolby Surround
Languages: English, French
Subtitles: English, Spanish
Extras: Running audio commentary with director John Amiel; two deleted scenes; alternate ending; "The Making of Entrapment" 13-minute featurette; two theatrical trailers; five television spots; production notes; cast bios
Video: The new anamorphic widescreen transfer (the initial no-frills DVD release of the film was not anamorphic) is absolutely gorgeous. The detail is strong, black levels are just right, and the colors are vibrant without being oversaturated. For a good example of how sharp and rich the picture looks, just watch the opening sequence that incorporates helicopter shots of the New York skyline at night, with all the buildings lit up in green and red for Christmas. There are no hints of artifacting or any other defects in the image. The film is nicely framed in its original aspect ratio of 2.35:1 without any noticeable edge enhancement.
Audio: The Dolby Digital 5.1 surround is as effective as one could hope for. The dialogue is crisp and easily understandable, and the surround sound is put to great use. Christopher's Young's thundering musical score will give any system's speakers a good workout. The surround speakers are also put to good use, but without being overtly noticeable. Overall, the soundtrack provides a rich, enveloping environment.
Extras: The newly released "Special Edition" contains a number of worthwhile extras. The production notes and featurette on the making of the film are fairly lightweight and forgettable. Director Jon Amiel's scene-specific running audio commentary, however, is definitely worth listening to. It gets a little dull during the film's slower sequences, but it offers a fascinating insight into the intricate robbery techniques used in the film. It's amazing how much of the high-tech equipment used in the film is based on actual working devices (some of it, of course, is pure fantasy that could never work, while other devices were designed specifically for the film). The deleted scenes that are included are interesting, but it is easy to see why they were left on the cutting room floor. Also, it is worth noting that the film's technical fetish extends to its particularly intricate animated menus, which are designed around locations used in the film, and can be viewed either normally, through night-vision goggles, or in 3-D.
©1999,2000 James Kendrick


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Please enter your travel dates.
Classic Double Room
- One Double Bed
Superior Double Room, Sea View
- One Double Bed
Suite, 1 Double Bed
- One Double Bed
Classic Triple Room, 1 Double Bed with Sofabed
- One Double and One Sofa Bed
Hotel in Pozzuoli between Averno and Lucrino Lakes in the heart of Campi Flegrei
This Pozzuoli hotel is located on the slopes of the natural oasis of Montenuovo. Not far from Temple of Serapis and Flavian Amphitheater, and Archeological Museum of Baia. Only 10km away from Naples.
Bar/lounge, beauty farm
At Villa Luisa Hotel Residence Beauty Farm recreational amenities include a sauna and a fitness facility. The hotel also features concierge desk, bicycle rental, diving services.
Balconies with views
Guestrooms open to balconies with ocean, sea, or mountain views and feature high-definition televisions with cable channels and air conditioning.
Hotel Amenities
- General
Villa Luisa has 22 rooms and 15 apartments, a bar, beauty farm with Turkish bath, Finnish Sauna, emotional showers, pool, and Jacuzzi. A small fitness center is also available for guests. Bicycle rental, concierge facilities, 4 star Diving service, massages, and beauty treatments can be booked. The beach is 50 meters from the hotel. Free Wi-Fi connection in the rooms.
- Total number of rooms - 37
- Number of buildings/towers - 2
- 24-hour front desk
- Air-conditioned public areas
- Babysitting or childcare (surcharge)
- Airport transportation (surcharge)
- Bar/lounge
- Beauty services
- Coffee shop or café
- Dry cleaning/laundry service
- Free breakfast
- Express check-in
- Elevator/lift
- Express check-out
- Business center
- Garden
- Health club
- Swimming pool - indoor
- Concierge services
- Area shuttle (surcharge)
- Rooftop terrace
- Luggage storage
- Laundry facilities
- Massage - spa treatment room(s)
- One meeting/conference room
- Multilingual staff
- Audiovisual equipment
- Room service (24 hours)
- Safe-deposit box at front desk
- Meeting facilities size (feet) - 538
- Free secure parking
- Sauna
- Secretarial services
- Security guard
- Spa services on site
- Spa tub
- Steam room
- Television in lobby
- Tour assistance
- Business services
- Complimentary newspapers in lobby
- Event catering
- Half board rates available
- Free Wi-Fi
- Porter/bellhop
- Meeting facilities size (meters) - 50
- Internet
Available in all rooms: Free Wi-Fi
Available in some public areas: Free Wi-Fi
- Parking
Free secure parking
- Room Amenities
- Pay movies
- Balcony
- Climate control
- Air conditioning
- Direct-dial phone
- Minibar
- Wake-up calls
- Private bathroom
- Hair dryer
- Extra towels/bedding in room
- Shower only
- Bidet
- Electronic/magnetic keys
- Iron/ironing board (on request)
- Welcome amenities
- Desk
- In-room safe
- Rollaway/extra beds available
- Digital TV service
- Flat-panel TV
- Free Wi-Fi
- Soundproofed rooms
- Complimentary toiletries
- Cribs/infant beds (surcharge)
- Connecting / adjoining rooms available
- Window opens
- Sea view
- Garden view
- Dining
The hotel restaurant is located at approximately 1.5 km away (transfer service available).
- Recreation
Villa Luisa is perfectly located in the heart of Campi Flegrei, just 50 meters away from Lucrino seaside. Walking distance to Lucrino and Averno lakes, just a few kilometers from Pozzuoli historical center and Baia. Visits to Archeological Ruins of Cuma, or at the Archeological Museum of Campi Flegrei available. Snorkeling at the Underwater Park of Baiae with 4 stars diving centre. Excursions to Naples and Campi Flegrei main attractions available.
In the summer an outdoor swimming pool is available nearby.
- Golf - miniature nearby
- Golf - driving range nearby
- Water skiing nearby
- Rowing or canoeing nearby
- Sailing nearby
- Snorkeling nearby
- Mountain biking nearby
- Golfing nearby
- Swimming nearby
- Boat launch nearby
- Tennis nearby
- Running track/path nearby
- Horse riding/rental nearby
- Scuba diving nearby
- Bicycle rentals on site
- Fishing nearby
- Ecotours nearby
- Spa
The spa centre is located within the hotel. The peace and relax that pervades the small gem of Villa Luisa creates an atmosphere in which the starry sky, the blue mosaic and the smooth contour of the rocks, melt together to create an enchanted environment, where you can take care of your body and rediscover your senses. Guests can relax and be pampered with massages, facial, and body treatments. Guests can choose several facial and body treatments, from regenerating with an immediate beauty effect to the classical beauty massage with white grapes, Ayurveda, Californian, connective, and hammam, sweet white chocolate scalp massage or classic mud therapy, and relaxations treatments. A section is available where you can have a Turkish bath, Finnish Sauna, emotional showers with aromatherapy, Jacuzzi, and a small recreational area.
- Body treatments
- Facial treatments
- Accessibility
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
- In-room accessibility
- Accessible bathroom
- Roll-in shower
Policies & Fees
- Check-in
Check-in time starts at 2 PM
- Check-out
Check-out time is 11 AM
- Payment types
Accepted at this hotel: American Express, Diners Club, MasterCard, Visa
- Pets
Pets allowed, Pet maximum weight in kg is 5
- General
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
Caters to couples
Caters to families
- Fees
- Unless otherwise indicated, the charges below are not included in the overall room price. If not included in the overall room price, they will be charged by the property at check-in or check-out. Charges can vary, for example, based on length of stay or the room you book. This property told us they will charge you for the following:
- All citizens of Italy will be charged the national value-added tax
We have included all charges provided to us by the property. However, charges can vary, for example, based on length of stay or the room you book.The following fees and deposits are charged by the property at time of service, check-in, or check-out.- Kitchen/kitchenette fee: EUR 50 per day
The above list may not be comprehensive. Fees and deposits may not include tax and are subject to change.
- Additional Details
Reservations are required for massage services and spa treatments. Reservations can be made by contacting the hotel prior to arrival, using the contact information on the booking confirmation. A resort fee is included in the total price displayed. One child 2 years old or younger stays free when occupying the parent or guardian's room, using existing bedding. Only registered guests are allowed in the guestrooms. The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation. Guests can arrange to bring pets by contacting the property directly, using the contact information on the booking confirmation.
- Details
Book a stay in a qualifying rate category and receive the following:
- Access to the wellness area
- Parking
- Internet access
- Daily breakfast


text:
A trip down Memory Lane
Harold "Ernie" Ernst of Princeton submitted this photo of his family on his family farm in Wythe Township, Hancock County, Illinois in 1926. Pictured are (front row, from left) William, George, John, Ruth and Dorothy; and (back row) his mother, Lora, holding Ernie.
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Russian iconography program offered at St. Elizabeth Seton Church in Bedford
Traditional Russian icon painting is one of the great artistic movements and has been a living and evolving art form for more than 1,000 years.
Russian icons reveal a deep inner spirituality, incomparably matched by an intrinsic rhythm of line and harmony of coloring.
Using numerous exhibits, including examples of her own work, Marina Forbes will examine the history of icon painting in Russia and will discuss the unique multiple nature of the icon as a sacred object, a product of an artistic tradition, a work of art with its own inherent aesthetic value, and a major example of our world cultural heritage. Anyone who has personal icons may bring them for examination and comment.
Forbes is a lecturer, historian and award-winning artist who has written extensively on Russian traditional arts, history and the rich tapestry of Russian culture.
She is licensed with the Hermitage Museum in St. Petersburg, and is featured on the New Hampshire Council on the Arts Rosters for Arts in Education and Traditional Arts.
Each year, Forbes leads cultural tours to Russia, where she regularly updates her scholarship, gathering photos and documents, visiting craft factories, GULAG sites and monasteries, and interviewing journalists, political figures and scholars on the current state of affairs in Russia.
She has a distinctive ability to generate energy and enthusiasm in the audience and to bring the rich fabric of traditional Russian art and folklore to life for children of all ages through memorable and thought-provoking programs.
Her talks are sometimes a little bit funny, sometimes a little bit sad, but always unmistakably Russian.
This program is free and open to the public and it was made possible through a grant from the New Hampshire Humanities Council.
For more information, call 332-2255.
Contact Information
The Bedford Bulletin
100 William Loeb Drive
Manchester, NH 03109
Ph: 603-206-7800
Fax: 603-206-7801
News, Obituaries, & Social Announcements
Christine Heiser, Executive Editor
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Cranmore To Offer Special Learn to Ski or Snowboard Package


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Topic closed. 1315 replies. Last post 2 years ago by Toy.
calabs box in tiers i had dp 18 19 congrats to all 3winners and posters.
love to nibble those micey feet.
Took a day longer than i expected...but the 20 pair did return....wtg to all posters/winners of 230
New pair watch next few draws/days: 91 92....see them in your w/o.......smash em hard...by the way.....Ga....can you send us a trip over here in NC....congrats to all that smashed the trip 2's last night
hope y'all paid attention Ga players....there's your 91 pair
WTG to all posters/winners of 891
Weezy *328 Str8* No Chaser
Congrats geeweezy
Congrats to all the posters and winners
Followers of 891
707 217 622 934 998
Good luck LP
I love 310 this evening
loving that 998 been playing it and the mate for a couple of months now
989,998,899 889,898,988 sometimes big sometimes the po man's way
.50 st all three ways
i LIKE 310 TOO!! AND 719!!!
217 and 622 are rochelles.
Hello Everyone my evening qp's today are 069, 066 and 123....what are your qp's and thoughts?
Congrats to all recent winners!! Qps for the eve are 212 and 420. Goodluck everyone.
Scared money don't make no money.
i like 361 783 763 743 413
How many more years does MARGARET have left in GA??? I want her GONE!! Lmao. Is there a way we can vote her off???? I hope she is reading this..
now that's funny! it's amazing because every time i go to play, 'SOMEONE IS TALKING ABOUT MARG" i hope really soon, i did well with rebecca.
qp's str 873 183 s/b 010 043 and 978
Thanks to all posters ! Lots of good info and numbers. I like the 998 as well been playing it. Like the 707 007 179 as well. Mslady thanks for posting your qps remember to flip your 6s ..... I also had 099 on my list of numbers to play. Watch out for 11 44 00 pr soon... Feel sum 6 could repeat shortly as well.good luck all!


text:
U.S. News Rankings
Ranking score and category | |||||
Graduate Information Technology Program Overview
Georgia Southern University, a public institution, does not offer labs for its online, graduate-level Computer Information Technology (CIT) program. All of the classes are recorded and posted online, so that students can access lecture material at their own convenience. The application deadline for the online graduate CIT programs at Georgia Southern University is July 31, and 100 percent of students are employed when they enroll.
U.S. News Rankings
Student Body
Enrollment | 45 |
Student gender distribution | |
Technology & Support
Recommended Internet speed | Cable |
Instructor response expected within (hours) | 24 |
24-7 live tech support | No |
More About Technology & Support
Paying for School
Part-time tuition (per credit, in-state) | $400 |
Part-time tuition (per credit, out-of-state) | $400 |
Full-time tuition (per credit, in-state) | $400 |
Full-time tuition (per credit, out-of-state) | $400 |


text:
We stayed from Thursday - Sunday in April 2012. We were not expecting much from a bathhouse for a room but thought it might be interesting. The room was surprising. Bed was comfortable, decent dressers and full bath with shower. Room had a TV as well with all the main channels and two resort porn channels. The room as expected... More
- Also Known As:
- Flex Cleveland Baths Ohio
United States >
Ohio >
Ranked #2 of 3 Specialty lodging in Cleveland


text:
Get the skills and education you and your community need to make a difference in creating the type of change we all need. Whether it's in-person or online, DFA has a training program that's right for you.
Headlines from Friday, December 6: Mandela's Death Stirs Sense of Loss Around the World — New York Times "A giant among men has passed
Headlines from Thursday, December 5: Obama Focuses Agenda on Relieving Inequality — Washington Post Historic speech sets agenda for rest of
Headlines from Tuesday, December 3: Budget Deal Optimism Emanates from Top House Appropriator — Roll Call Congressmen are hopeful…
McDonalds at it again: now helping workers tip for massages, au pairs, personal trainers, pool cleaners. On $9/hr?! http://t.co/3kRl67ow8a
No plans, just "no." #uniteblue http://t.co/1xmpXyHZWs
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Indiana Jones and the Last Crusade
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The third installment in the widely beloved Spielberg/Lucas Indiana Jones saga begins with an introduction to a younger Indy (played by the late River Phoenix), who, through a fast-paced prologue, gives the audience insight into the roots of his taste for adventure, fear of snakes, and dogged determination to take historical artifacts out of the hands of bad guys and into the museums in which they belong. A grown-up Indy (Harrison Ford) reveals himself shortly afterward in a familiar classroom scene, teaching archeology to a disproportionate number of starry-eyed female college students in 1938. Once again, however, Mr. Jones is drawn away from his day job after an art collector (Julian Glover) approaches him with a proposition to find the much sought after Holy Grail. Circumstances reveal that there was another avid archeologist in search of the famed cup -- Indiana Jones' father, Dr. Henry Jones (Sean Connery) -- who had recently disappeared during his efforts. The junior and senior members of the Jones family find themselves in a series of tough situations in locales ranging from Venice to the most treacherous spots in the Middle East. Complicating the situation further is the presence of Elsa (Alison Doody), a beautiful and intelligent woman with one fatal flaw: she's an undercover Nazi agent. The search for the grail is a dangerous quest, and its discovery may prove fatal to those who seek it for personal gain. Indiana Jones and the Last Crusade earned a then record-breaking $50 million in its first week of release. ~ Tracie Cooper, Rovi
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Los Locos
Mario Van Peebles wrote, produced, and starred in this follow-up to his 1993 Western Posse. Chance (Van Peebles) is a cavalry scout in the old west who accidentally gives his battalion wrong directions going into a battle. Not being a forgiving bunch, the soldiers beat Chance, tar and feather him, and leave him to die in the desert. However, Chance is found by Buck (Paul Lazar), who asks him if he's the young god Icarus, who fell to Earth after flying too close to the sun. It seems that Buck is an inmate at an insane asylum that's part of a nearby convent; Chance is brought back to the convent, where the nuns nurse him back to health. Chance learns that the Mother Superior (Jean Speegle Howard) is in poor health, and she asks Chance to lead the nuns and their charges to a safer mission 100 miles across the arid plains. Mother Superior dies only a few hours later, and the cruel Sister Drexel (Rusty Schwimmer) takes charge of the flock. Not eager to take her abuse, Chance goes his own way, but when he encounters the disturbed men of the convent, led by the self-proclaimed President (Rene Auberjonois), he realizes that he must help them if they are to survive. Los Locos was also distributed as Los Locos: Posse Rides Again, though while both are set in the old West and star Mario Van Peebles, he does not play the same character and the two films have no narrative connection. ~ Mark Deming, Rovi
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No Country for Old Men
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When a Vietnam veteran discovers two million dollars while wandering through the aftermath of a Texas drug deal gone horribly awry, his decision to abscond with the cash sets off a violent chain reaction in a stripped-down crime drama from Joel and Ethan Coen. Llewelyn Moss (Josh Brolin) has just stumbled into the find of a lifetime. Upon discovering a bullet-strewn pickup truck surrounded by the corpses of dead bodyguards, Moss uncovers two million dollars in cash and a substantial load of heroin stashed in the back of the vehicle. Later, as an enigmatic killer who determines the fate of his victims with the flip of a coin sets out in pursuit of Moss, the disillusioned Sheriff Bell (Tommy Lee Jones) struggles to contain the rapidly escalating violence that seems to be consuming his once-peaceful Lone Star State town. Woody Harrelson, Javier Bardem, and Kelly MacDonald co-star in a distinctly American crime story that explores timeless biblical themes in a contemporary Southwestern setting. ~ Jason Buchanan, Rovi
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North Country
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A fictionalized account of one of America's most groundbreaking sexual harassment lawsuits comes to the screen in this hard-hitting drama. In the late '80s, Josey Aimes (Charlize Theron) fled her abusive husband, and needed to find a way to support her two children. Aimes returned to her hometown in Minnesota and followed the lead of her old friend Glory (Frances McDormand), who had bucked tradition and found a job in the iron mines that had long provided employment for much of the community. Aimes found honest labor and a living wage working the mines, but she also discovered she was working with men who were uncomfortable working with women (whose right to work in the mines had been mandated by law almost 15 years prior), and didn't care to show them much respect. However, as Aimes found herself the growing target of sexist jokes and abhorrent behavior, she found that many of her female co-workers were reluctant to stand beside her, afraid of losing a good-paying job at a time when they were increasingly hard to find. But as a personal crisis became a public war of words, Aimes became the center of a nationwide controversy when she attempted to file a class action sexual harassment suit against the mine owners, which put her and her family in a position of scrutiny beyond her worst expectations. North Country also stars Sean Bean, Sissy Spacek, and Woody Harrelson. ~ Mark Deming, Rovi
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Powwow Highway
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The road movie gets a smart update with this seriocomic tale of two Cheyenne men traveling from their reservation in Montana to New Mexico. For one of them, Buddy Red Bow (A Martinez), a quick-tempered activist, the journey is a practical one; his sister has been arrested and he is the only family member who can help her out. Buddy has no transportation, so he's forced to ride with Philbert Bono (Gary Farmer), a phlegmatic hulk of a man who is using his 1964 Buick as a vehicle for a spiritual journey of his own. Philbert's easygoing ways and insistence on frequent stops to meditate prove irritating at first to Buddy, but the men reach an accommodation as the trip wears on. Buddy comes to see that blaming the white man and what he sees as system rigged against Native Americans is distracting him from his true mission: to better understand himself and his place in the world. ~ Tom Wiener, Rovi
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Suburban Commando
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A vehicle for popular wrestling celebrity Hulk Hogan, Suburban Commando is an inoffensive science-fiction fantasy. Hogan plays Shep Ramsey, a well-sculpted if somewhat dimwitted intergalactic hero. On vacation from fighting crime on other planets, he has a fight with an alien enemy and his spaceship is damaged. He seeks refuge on Earth until his ship can be revived. Trying to look inconspicuous as an ordinary human being without special powers, he is befriended by a suburban family headed by Charlie Wilcox (Christopher Lloyd) and his wife Jenny (Shelley Duvall). Ramsey's stay isn't peaceful because he has such a keen sense of justice, which he dishes out to muggers, reckless drivers, and even smart-aleck paper carriers. In the end, he has to defend the family against his bold nemesis. ~ Michael Betzold, Rovi
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The Astronaut Farmer
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An aspiring astronaut who was forced to forsake his dream of exploring space in order to save the family farm begins building his own personal rocket as a means of reaching the stars in this quirky rural drama starring Billy Bob Thornton. There once was a time when Charlie Farmer (Thornton) seemed like a shoo-in for NASA's esteemed astronaut training program. An Air Force pilot who held a degree in aerospace engineering, Charlie was well on his way to blasting off when a family crisis grounded his celestial aspirations. Of course, Charlie's feet may be firmly planted on terra firma these days, but his thoughts are constantly ascending into the clouds and beyond. It's been ten long years since the man who would navigate the stars began tending to the family farm, and during that time, Charlie has dedicated every moment of his spare time to building the rocket that will one day launch him into space. Though his devoted wife, Audie (Virginia Madsen), the pair's three children, Sunshine, Stanley, and Shepard, and even his father-in-law, Hal (Bruce Dern), all support Charlie in achieving his lifelong goal of becoming the nation's first independent astronaut, a last-minute hitch on the eve before the big launch unexpectedly stalls Charlie's countdown. It seems that Charlie's quest to obtain a substantial amount of high-grade rocket fuel has attracted the attention of not only the FBI, but the CIA, the FAA, and the United States military as well. Now, as the powers that be attempt to ground Charlie, citing concerns for civilian safety, a media frenzy descends upon the once-quiet farm as the reluctant renegade hero vows to show his children just how far one's courage and willingness to pursue one's own ideals can get a person when one refuses to give up hope. Northfork writing/directing duo Mark and Michael Polish team up to tell the tale of a man considered an oddity by his neighbors, a criminal by the government, and an inspiration by the media and the people. ~ Jason Buchanan, Rovi
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Thermo Scientific EZ-Link NHS-Biotin is the simplest NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for general intracellular labeling.
NHS-Biotin is N-hydroxysuccinimidobiotin. It is the shortest of three similar EZ-Link NHS-Biotin Reagents that enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules in solution. Differing only in their spacer arm lengths, the three NHS-ester reagents offer researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Because they are uncharged and contain simple alkyl-chain spacer arms, these biotin compounds are membrane-permeable and useful for intracellular labeling.
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
N-Hydroxysulfosuccinimide (NHS) esters of biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.
Varieties of biotin NHS-ester reagents differ in length, solubility, cell permeability and cleavability. Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF. Sulfo-NHS biotins (and those with pegylated spacers) are directly water soluble but not membrane permeable. Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein.
Biotinylation Reagents Selection Guide Review of Biotinylation Methods and Applications Chemistry of Crosslinking (and Labeling Reagents)
All Amine-Reactive Biotinylation Reagents All Biotinylation Reagents Biotin Quantitation Reagents and Kits Avidin, Streptavidin, NeutrAvidin Affinity Resins


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