text:
7 Night Vita Health Weight Loss Programme.Some of us can indulge in whatever we like to eat without putting on weight. Most of us though, aren't so fortunate.
Increased intake of energy-dense foods that are high in fat, salt and sugars but low in vitamins, minerals and other micronutrients is compounded by badly timed meals. Put that together with a decrease in physical activity due to sedentary forms of work, changing modes of transportation and increasing urbanisation, it's easy to see why weight is a big problem for many people.
But it's more than that. Many people's diets result in inflammation at cell and tissue level. This in turn can lead to raised stress hormones and an excess of insulin, while a deficiency of certain minerals can lead to sluggish cell metabolism.
At Vita Health we identify the hormonal, mineral and inflammatory imbalances that can contribute to weight loss resistance and then correct them.
Naturally, diet has a big part to play. At the heart of our healthy-eating programme is the Mediterranean diet, proven to be the most effective for weight loss and a healthy heart. This also helps lower rates of diabetes and depression.
Our food is sourced from the fertile island of Sicily and our menus, designed by Amelia Freer and Fulvio Pierangelini, are rich in fish, olive oil, whole grains, fruit and vegetables, flavoured with herbs grown in gardens of Verdura resort. Download the Vita Health Weight Loss Programme Overview here
Enquiries and Reservations


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The public beta is finally here:
The beta includes different branches of the Philippine Army:
The Marines
The Army
Cause of lack of time, but on the other side I didnt want to exclude the army in the first release I made 3 soldiers of the 1st Tabak Division and one in the new Digital camo.
Philippine National Police (PNP)
I did also include one Pilot of the 206th Tactical Helicopter Squadron "Hornets"
Note that this only is a public beta and not the final.
The demo includes about 35 different soldiers in different loadouts.

subset: commoncrawl


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Biaya penambahan orang dalam kamar mungkin berlaku dan berbeda-beda menurut kebijakan hotel.
Sewaktu check-in diperlukan tanda pengenal dengan foto yang resmi dikeluarkan oleh pemerintah dan kartu kredit atau deposit tunai untuk menutup biaya tak terduga.
Pemenuhan permintaan khusus bergantung pada ketersediaan sewaktu check-in dan mungkin menimbulkan biaya tambahan. Permintaan khusus tidak dijamin akan terpenuhi.
Biaya dan deposit berikut mungkin ditagihkan oleh properti saat memberikan layanan, check-in, atau check-out.
Daftar di atas mungkin tidak lengkap. Biaya dan deposit mungkin belum mencakup pajak dan dapat berubah tanpa pemberitahuan sebelumnya.
Biaya penambahan orang dalam kamar mungkin berlaku dan berbeda-beda menurut kebijakan hotel.
Sewaktu check-in diperlukan tanda pengenal dengan foto yang resmi dikeluarkan oleh pemerintah dan kartu kredit atau deposit tunai untuk menutup biaya tak terduga.
Pemenuhan permintaan khusus bergantung pada ketersediaan sewaktu check-in dan mungkin menimbulkan biaya tambahan. Permintaan khusus tidak dijamin akan terpenuhi.
"Not fancy but adequate"
Convenient if your going to sports events at BGSU campus. Clean. Walkable to restaurants and shopping. Free breakfast rates a C-.Ulasan otentik tamu Hotels.com
"Over night stay.."
The hotel was conveniently located next to the places we needed to visit. The hotel room was clean and ample room space with double beds to accommodate upto 4 guests . The price was very reasonable compared to other hotels near by. Room included a continental breakfast for all guest, great for a quick bite and coffee. Friendly staff to answer any questions. […] Unfortunately, the swimming pool is outdoors and was closed due to the weather. Baca selengkapnyaUlasan otentik tamu Hotels.com
The ammenities and in room refrigerator are great and beds are very comfortable. The continental breakfast is very basic and the hotel is showing its age.Ulasan otentik tamu Hotels.com
"pretty old but clean."
this days inn is very close to the stadium which was great. the room and hotel in general were outdated, but our room was clean and everything worked fine. for why we were there this hotel suited us fine.Ulasan otentik tamu Hotels.com
"Got what I paid for."
Had to be moved from my original room because I couldn't get the door open. The second room was OK.....but just before I left I had to kill a rather large spider on the wall. Overall condition of hotel was rather disappointing......poor paint job, bath rooms rather basic and out dated. I will not stay there again.Ulasan otentik tamu Hotels.com
"Dirty rooms"
This was the dirtiest hotel I have ever stayed in. It looked like the floors had never been vacuumed, especially under the beds. I would never stay in this hotel again.Ulasan otentik tamu Hotels.com
"No windows"
If you are waiting for the sun to come up before you wake up, don't stay here. The rooms at this place has no windows, very bad internet connection and a trick way to open the front door. The room is good for sleeping and has a refrigerator.Ulasan otentik tamu Hotels.com
It was clean, employees were very nice and very accomodating and considerate, rates through hotels.com were a good deal, electrical outlets were not all working in the room (I moved coffee pot into bathroom to operate; narrow ledge to reach outlet there). I had some concern about "octopusing" many appliances into one outlet. All in all it was a pleasant […] place to crash every night and I wasn't there to lounge in the environment. I felt reasonably safe for a single female travelling alone. Baca selengkapnyaUlasan otentik tamu Hotels.com
Everything was good until i found a bug in the bed on the next dayUlasan otentik tamu Hotels.com
"This hotel should have no national affiliation"
Rotted doors, broken door locks, toilet never stopped running, broken vanity lights, awful condition. To put it simply, this hotel makes burned out trailers look like the Hilton. How in the world Days Inn allows its name to be on the sign is a mystery to me.Ulasan otentik tamu Hotels.com
Bad! Air did not work. Had to wait on the girl at the counter to finish her homework before she would answer any questions i had.Ulasan otentik tamu Expedia
"Not a great stay"
I have stayed at Days Inn locations before. This was by far the worst location. The room smelled like smoke/cleaning products. To the point that the clothes we slept in have a terrible odor still, as well as a blanket and pillow my daughter brought. The whole property is older and seems woefully inadequate for the amount of money I spent ($94 including […] taxes). The swimming pool was "closed" even though anyone could have walked through the gate. When I asked at the office if the pool was actually closed (a sign was at the gate), they said yes, and that "the pool has been needing to be fixed for a few weeks now". Lovely. Baca selengkapnyaUlasan otentik tamu Expedia
This hotel should be condemned. When we walked into the room the smelll was just awful. On further inspection we realized that the sheets were not changed nor clean. Just horrible and we checked out right after checking in......Ulasan otentik tamu Hotels.com
"Days Inn, Bowling Green, OH"
I was VERY unhappy with this hotel. I had asked for a double bed/non-smoking room. I was put in a suite (of sorts) which reeked of smoke. There were cigarette burns in the carpet and carpet was very dirty. The shower did not work. This hotel needs to upgrade their property.Ulasan otentik tamu Hotels.com
"Can you guess what's missing?"
The room is just adequate, but the fact is it's near the freeway and there are NO windows in many of the rooms! Our first room felt like a prison cell. And it looked like someone had taken a crowbar to the door. They moved us, but we had to go to the second floor.Ulasan otentik tamu Hotels.com
"disappointing experience"
towels had very noticable stains/ carpet needed to be vac./ trash pick up a 3am loud banging dumpstersUlasan otentik tamu Expedia
"decent accmodations for overnight"
the hotel staff was courteous,and helpful.the room was comfortable,could have been a little cleaner.WE have no complaints about the overall stay,however, our next door neighbors were very loud, playing their music at all hours.we had to get the front desk to ask them to lower it 3 times.perhaps a reminder of courtesy hours needs to be in the contract […] and the rooms. Baca selengkapnyaUlasan otentik tamu Expedia
"Convenient to BGSU campus."
We knew this was an older hotel and had some problems with the room keys, but other than that it was a good place to stay for the night.Ulasan otentik tamu Expedia
"It was a lousy place"
We didn't stay that's how bad it wasUlasan otentik tamu Hotels.com
"beyond belief"
this hotel has not been renovated since it was built,floors are saking,dirty,tv was out of the 70)s poor reception.they have two ice makers,both of which were out of order..i have seen truck stops in better condition.Ulasan otentik tamu Expedia
"The place needs work"
Though my room was generally clean, the carpets were in serious need of cleaning, the door lock was partially broken from someone previously breaking in, and the microwave didn't work. I would not recommend staying here to friends or family.....the entire place was generally in pretty poor shape and need of updating....Ulasan otentik tamu Hotels.com
"scared to sleep"
So I don't check in till 2 a.m. after driving a long way we had to go to Bowling Green at 7 am in the morning . As soon as we got a room and were unloading our luggage we were confronted by three drunk construction workers that were partying in the parking lot.The room we were given was locked from the inside.Scared to walk past the partiers I called […] the front desk and explained my situation.The three guys then came up and approached us from 2 diff sides the hotel worker finally came and let us into the room next door with the drunk guys who just picked up our luggage and tried to help. The hotel attendant said they are just construction guys. Once in the room there was no additional lock on the door. My teenage daughter said it will be ok mom as she drung the Desk and chair in front of the door. I slept for 3 1/2 hours with one eye open . What should have been a fun experience the next day at the college orientation turned out difficult due to lack of sleep. The next morning the front desk attendant acted like it was no big deal.it to me was a big deal if all this would not have happened I would have got the room for a couple days so we could rest! Not to mention the pool was not even open and it is the middle of june! Shame shame hampton here I come for the next four years to visit my student! Baca selengkapnyaUlasan otentik tamu Hotels.com
"Not a good experience"
I had booked 3 rooms and only one room was ready at check-in. So we all had to get ready for a wedding in one room. After 4:00 only the second room was ready and we had to leave and ask for the keys for the third room because it still wasn't ready. We reserved one room with a king size bed and it was only a queen. Another hotel got busted for illegal […] things and I think they moved their business to this hotel because I could hear them all night long. Not a good experience at all. Baca selengkapnyaUlasan otentik tamu Expedia
Rooms were cleanUlasan otentik tamu Hotels.com
Our stay was adequate but we probably wouldn't stay there again. Your staff was courteous and helpful, but the worn, outside appearance of the motel wasn't very appealing. The room was also adequate but was small and dark. For the short amount of time we were there, this was okay.Ulasan otentik tamu Hotels.com
"Good night"
The room was very quite considering it was facing the major highway. The noise from the traffic did not keep us awake and we both rested soundly. The eveing of our arrival due to bad direction from AAA we called the hotel and the clerk on duty was able to direct us to the hotel.Ulasan otentik tamu Expedia
"VERY unvomrotable bed!!!"
We had a double room with 2 double beds. One of the beds in the room was SO lop-sided that I had to prop a pillow behind my back to avoid rolling onto my daughter sleeping next to me all night! - The rooms cleanliess was lacking as well (especially around the all / edges of the carpet )Ulasan otentik tamu Expedia
"You get what you pay for"
This is an old beat up hotel property. My room was the single queen (non-smoking, thankfully). My room used to house a kitchenette, but had a recent mini fridge and a microwave where the stove and fridge used to be. The cabinets and range hood remained. My permanent resident next door neighbors sometimes fought loudly. There were no drapes or curtains […] on the window -- only blinds -- which had no problem letting the security lights beam into the room at night. Also, the comforter on the bed was old, unclean and ratty. The TV's remote control was sticky.. had to clean that. The only plusses in my room was the sheets were clean, the pillows were comfy, and the mattress wasn't terrible. Overall, an old dirty room. Baca selengkapnyaUlasan otentik tamu Hotels.com
"Get what you pay for ---- unacceptable"
The first room we were put in did not have a lock on the door. It was room #222. The second room #223 had no windows and felt light light a bomb shelter. We stayed 2 nights and did not recieve any housekeeping had to call front desk for some fresh towels.Ulasan otentik tamu Hotels.com
"Clean but old"
Looks like you've walked into the 1960s.Ulasan otentik tamu Hotels.com
"One night stay for Soccer Tournament"
The price was high for the condition of the place. The room had a funny smell when we walked, like 10 cans of freshener were dispersed inside before our arrival.Ulasan otentik tamu Expedia
it is an ok stay, uneventful. Door is a little hard to close.Ulasan otentik tamu Hotels.com
"Needs an update"
Very run down. Bed very hard. Overpowering smell of room spray.Ulasan otentik tamu Expedia
"won't be back"
Needs renovated. security issues with doors. Wet urine spot on bed approximately 3'x2' that was just covered up with a mattress pad. Changed rooms. New room was better, but I won't be back.Ulasan otentik tamu Hotels.com
"Perfect overnight stay to visit university"
Served its purpose, only problem was the back door did not have a lock, just a hinge to pull over. Otherwise it was good.Ulasan otentik tamu Hotels.com
"Basic accomodations."
Easy to get to from the highway, though the entrace from the main road is a little tricky. While the price is probably comparable to the local market, I've enjoyed nicer accomdations just an hour north for $25 less, so thought the overnight rate was a bit high for basic lodging. Nice that it was walking distance to restaurants. Breakfast had fresh fruit, […] cereal, waffles, breads...the basics. Room had small fridge & extra sink. Baca selengkapnyaUlasan otentik tamu Hotels.com
Front desk guy was greatUlasan otentik tamu Hotels.com
I stayed in RM. 111. I was very Satisfied. I require CLEAN!!! and it was. You just can't beat it for the $$$. Some reviews Ripped this Hotel. My Room was totally Fine.Ulasan otentik tamu Hotels.com
"close to Bowling Green University"
Front Desk very friendly and professional. Price was great. Room was very clean. Typical continental breakfast. No complaints.Ulasan otentik tamu Hotels.com
"easy access to college funtions"
was a nice place to catch some sleep, didnt have time to enjoy it for anything else.Ulasan otentik tamu Hotels.com
"Nasty rooms"
Very dirty. Stained sheets. Coffee maker hadn't been cleaned in months.Ulasan otentik tamu Hotels.com
"close to university"
hotel staff was nice and pleasant, felt very welcomed. The only thing is maybe that rooms could have windows.Ulasan otentik tamu Hotels.com
"Good hotel for the price!"
Our rom was very clean, the beds were comfortable and the up keep of the room was relaxing. The staff was friendly upon check in and explained check out time and informed us of the continental breakfast and the time it was served. Very nice place to stay for the price.Ulasan otentik tamu Expedia
"Close to BGSU and sport bar"
Condering the room was only $54, can't really complain. The beds were comfortable and clean. Bathroom was very clean. Doors to the rooms are very old, but like I said get what we paid for. Overall, satisfied but don't expect much. Check in fast and easy.Ulasan otentik tamu Hotels.com
"changes for bettter hotel stay"
1st room they put us in..so cold even after heat was on for 1/2 hour..walls were cement..2nd room was warmer..but other door exit was not sealed well..drafty...heater was very loud..bed was not what i wd consider queen..more full..too short for 6' tall person(my husband)..alota stains on mattress sides and bed spread..shower head came off..light fixtures […] in bath room did not have a covering over light bulbs..moisture hits em when ur in shower or little ones touch em if they crawl on sink..ouch!Staff very friendly...breakfast..coffee cold..tables needed to be cleaned more often.. Baca selengkapnyaUlasan otentik tamu Hotels.com
"Good hotel for a lower cost"
Nice place to stay for the price. The front desk clerk was very friendly and made us feel welcome.Ulasan otentik tamu Expedia
"Decent place"
decent room, a bit drafty thoughUlasan otentik tamu Hotels.com
"Bad Night Stay"
This hotel was in poor shape...first room we checked into we had to turn on the heat and it was cold outside. We were gone for over 2 hrs and the room was still cold with the heat turned all the way up. They moved us to another room and we again turned on the heat then left...came back and it was sooo hot we had to keep adjusting it all night long to […] hot or to cold no in betweens...bed was lumpy with an old blanket that was worn out and scratchy. We will never stay here again! Baca selengkapnyaUlasan otentik tamu Hotels.com
"no 2 star for sure"
If all you are looking for is a CHEAP place to stay look no more.Breakfast should not even be offered. Was beyond terrible. Roim smelled bad. Didnt want to use shower. UggghUlasan otentik tamu Expedia
Very disappointed with this hotel. Toilet was clogged and main security problem the door would not latch without all my body weight and pushing to latch door. Upon leaving I could not close the door.Ulasan otentik tamu Hotels.com
Ulasan dan peringkat yang ada di situs ini merupakan pendapat pribadi dari pelanggan dan suplier yang bersangkutan; ulasan tersebut tidak mencerminkan posisi Hotels.com. Baik Hotels.com maupun pihak yang terlibat untuk pengadaaan konten ulasan tidak dapat dimintai pertanggungjawaban untuk kerugian dalam bentuk apa pun yang disebabkan dari penggunaan ulasan ini


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SOURCE Texas Renewable Energy Industries Association
Transforming America's Energy Future with Renewable Energy, Natural Gas and Energy Efficiency
SAN ANTONIO, Oct. 17, 2013 /PRNewswire/ -- America is in the midst of a low carbon energy revolution. New energy technology has delivered much needed energy independence to the U.S. In San Antonio, nationally recognized speakers showcase the "All-of-the-Above" energy world and impacts of drastically reduced carbon emissions at Texas Renewables 2013. The conference and exhibit is organized to provide a solid understanding of forces at play, of economic and environmental benefits and strategies on continued growth for all Texas energy providers. Texas Renewables 2013 runs November 11 – 13 in San Antonio, TX at the Omni Colonnade, 9821 Colonnade Blvd, San Antonio, TX.
This exciting new conference experience includes TED style talks, video transitions, industry CEO panels and an exceptional lineup of speakers. Leading keynotes and speakers include:
Big picture topics include:
Dr. Arvizu delivers a keynote on Renewable Energy in an "All-of-the-Above" World. Arvizu is one of the world's leading experts on renewable energy and sustainable energy. As NREL's laboratory director, he has established and implemented a new institutional strategy to position the lab for higher impact and contributions to national and global energy challenges.
Reicher addresses the question, "Are renewables and fossil fuels in an 'All-of-the-Above' energy world a fit or a fight?" Following Reicher keynote, a panel expands on the topic. Dr. Jurgen Weiss, Principal, The Brattle Group reviews aspects of a report his group researched and published in June on the interplay between natural gas and renewable energy resources in the Texas electric energy market. Regarding the report former Texas senator Kip Averitt, Chairman, Texas Clean Energy Coalition said, "Going forward, Texas will reap huge economic and environmental benefits as the market learns how natural gas and renewables complement each other."
Texas Renewables Conferences organized annually by Texas Renewable Energy Industries Association (TREIA), represent hundreds of member companies, organizations, and individuals providing products, services and information within Texas' growing renewable energy sector. Founded in 1984, TREIA is a key trade and advocacy organization headquartered in Austin, Texas. TREIA http://www.treia.org/ is the oldest renewable energy non-profit trade organization in Texas whose work promoting development of renewable resources and their wise use has spanned a quarter of a century. For more conference information on Texas Renewables 2013 go to www.TexasRenewables.org or call 830-456-1341.
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Support the Burkle Center
Created in 1979, the Burkle Center for International Relations at UCLA fosters research and analysis on U.S. foreign policy and international relations, brings the brightest minds in these fields to UCLA and to the Los Angeles community, and encourages faculty and students to explore and shape debate on global issues. The Center sponsors conferences, lecture series, and other events that bring world leaders and internationally renowned scholars to UCLA. The Center hosts Senior Fellows, including General Wesley K. Clark (ret.), sponsors research, administers fellowship and scholarship programs, and provides web-based publications, news, podcasts, and video op-eds that raise awareness about important issues in international relations.
To maintain and enhance the quality of our research endeavors and public events over the coming years, the Burkle Center requires substantial support. Private philanthropy can make a critical difference in our ability to attract renowned speakers, promote cutting-edge research, support faculty and students, and provide forums for scholarly and public discussion and debate.
Please consider supporting the Burkle Center for International Relations.
Core financial support is provided by an endowment generously given by Ronald W. Burkle and by funding from the University of California. Additional funding comes from an endowed scholarship provided by Alice Belkin and a grant from IGCC for Burkle Forum events. The Center accepts additional sources of support in the form of private donations and grants.
Options for support include:
- Support public lectures or other events
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Options for engagement include:
- Help publicize the Burkle Center and its activities
- Offer suggestions for future speakers or programs
- Volunteer or speak at the Center's Career Panels
- Keep us informed of other Los Angeles-based international relations activities


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This is by far one of the nicest hotels we ever stayed in. Very clean, staff is nice. its right by all the action. We had breakfast at the restaurant there, Q's and it was very good. Everything at the Boulderado is top notch. Comfortable beds. Beautiful woodwork. Cathedral like ceilings. Very quiet room.
- Also Known As:
- Boulderado Hotel Boulder
- Boulderado Hotel
- Official Description (provided by the hotel):
- This hotel has been in the city's National Historic Register since 1909. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Expedia, Hotels.com, Booking.com, Orbitz, Travelocity, Amex, Priceline, EasyToBook, Venere, Fareportal , Otel and Tingo so you can book your Hotel Boulderado reservations with confidence. We help millions of travellers each month to find the perfect hotel for both holiday and business trips, always with the best discounts and special offers.


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Detection and Quantification of Recombinant ProteinsMicroMol's BioReference Extracts and Kits are designed for the detection of recombinant proteins and residual proteins from the production strain.With the GST and HIS kits you can detect and quantify the protein your expression system should produce. The kits contain a reference protein of defined quantity and a ready-to-use anti-tag antibody or conjugate. Tested for ELISA and Western blotting.The BL21 (DE3), HB101 and W3110 kits are designed for the detection of residual proteins of the expression strain. This is very important for the validation of purification methods. The relevant document rules for pharmaceutical preparations urge the producer of a recombinant protein to confirm that the purification process is able to remove all impurities from the preparation. Our kits are designed to do this by Western blotting or ELISA. They contain raw extracts of the most common production strains and an adequate antiserum that was prepared against whole cell extracts.Also see the section "Services" for more information.
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45 CFR 301.12 - Submittal of State plan for Governor's review.
§ 301.12
Submittal of State plan for Governor's review.
The State plan must be submitted to the State Governor for his review and comments, and the State plan must provide that the Governor will be given opportunity to review State plan amendments and long-range program planning projections or other periodic reports thereon. This requirement does not apply to periodic statistical or budget and other fiscal reports. Under this requirement, the Office of the Governor will be afforded a specified period in which to review the material. Any comments made will be transmitted to the Office with the documents.
(Approved by the Office of Management and Budget under control number 0960-0253)
[40 FR 27147, June 26, 1975, as amended at 51 FR 37730, Oct. 24, 1986]
Title 45 published on 2012-10-01
no entries appear in the Federal Register after this date.
This is a list of United States Code sections, Statutes at Large, Public Laws, and Presidential Documents, which provide rulemaking authority for this CFR Part.
This list is taken from the Parallel Table of Authorities and Rules provided by GPO [Government Printing Office].
It is not guaranteed to be accurate or up-to-date, though we do refresh the database weekly. More limitations on accuracy are described at the GPO site.


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You're watching...
How will Clinton answer Benghazi questions?
All-Star panel weighs in
Jan 8, 2013
You're watching...
All-Star panel weighs in
Jan 8, 2013
Also in this playlist...
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It would be unfortunate.
I think if in pursuit of this issue.
Which was highly politicized.
The senate would hold up the nomination of John Brennan to be director of the Central Intelligence Agency.
I'm not going to confirm John Brennan or anyone else until the administration.
Shares information with the congress about -- deleted references to al-Qaeda three weeks before the election.
I think it was purposely done and I want to know who did it and why before we move forward.
Senator Lindsey Graham saying this man the president's pick to be the next CIA director John Brennan.
Will have trouble in the senate he's going to hold up this nomination until he gets answers on Ben Ghazi.
This is just part of this investigation.
On the day nearly four months to the day after investor Chris Stevens and three others were killed in Benghazi Libya.
That a Tunisian is let go.
In Tunisia one of the only suspects being held of the Tunisian government saying a court saying there was lack of evidence there.
According to his lawyer.
What about all of this let's bring in our panel Tucker Carlson editor of the daily caller dot com AB Stoddard associate editor of the hill and syndicated columnist Charles -- -- talk.
Well I mean I think from the administration's -- he was probably ashamed that Secretary Clinton didn't testify earlier she will testify in the 22 apparently now.
But she'll have to answer questions not just about what happened before the attack and why security wasn't adequate to protect the ambassador and the three others.
What's happened subsequently and not just the removal as senator Graham said of the -- al-Qaeda from the report.
But why after four months there is no one in custody and apparently no one being seriously pursued in this attack keep in mind there -- security cameras.
At the consulate in -- -- that we have learned through a number of accounts capture the images of people who committed this violence and yet.
Apparently -- particular -- that's a massive failure and that's one of the things are gonna answer for when she goes up to help.
And it.
I am.
It would be surprised actually if given the fact that senator Graham wants to hold up a John Brennan's nomination.
And the fact that Hillary Clinton has really gone -- overweight should just stay out of the Ben Ghazi back and forth.
And might her -- not be entirely aware of why the CIA talking points were switched around.
I would be surprised -- the administration didn't come out before she testifies to explain somehow.
What happened here said that John Brennan's nomination proper procedure can go through.
He can be confirmed incidents secretary on her way out of her job.
Can come in and testify I really highly doubt.
That she is involved with -- -- be editing of this talking points and I would be surprised if it's really left on her shoulders to come and explain.
But there will be a lot of questions that -- -- attitudes about security well not only that but.
I thought she tells the father of the slain.
Seal at the service that they're going to go after the guy who made the video according to the father.
There's going to be a time and that is her responsibility.
I doubt that that.
That is going to be after her to come up an explanation of what happened in this area talking points is Susan Rice used.
And why they were scrubbed and how she got them and who prepared them for her I think that's something the administration.
It would be wise of them I would imagine that they would get that -- Before she sits in the hot seat on all those other topic she has said she's responsible she's gonna answer about the recommendations of the report.
Included what needs to be carried out in the next administration under the State Department but -- the CIA talking points issue.
I I imagine someone else's problem and I think it's going to be revealed before she's there.
But what the first things have to explain is why for three months after the event.
Two months after the event.
And before her injury she didn't say a word.
She is ahead of the department BM brand ambassador work for her.
He didn't work for Susan -- And she said the buck stops here and as she's had nothing.
We don't know what she knew about security before.
We don't know what she was doing during the hours of 78 hours of the attack.
And we don't know what -- role was in the -- of stories entails afterwards.
The other thing that was remarkable to me.
Jay Carney I mean -- prejudice they are about this story.
Carney as we just said the highly politicized.
It's not.
Political if you ask -- administration.
To explain what happened.
That's all that the critics have -- since the beginning.
What happened before why were all the warnings ignored what happened during those seven hours the one thing that we should hear from -- it is.
As to close advisor to the president on terrorism.
What was the president engaged -- in the seven what did Brendan though.
What did he tell the president what orders keep getting given where they carried out there was so much unknown you know I'm not surprised that we have to.
Let go the only suspect there is zero interest in the White House.
For what they you know the story Lisa Vivian really tracked him down I would bring them.
Could just as we know the leader of the attack was a certain.
I hammed who could tower.
Who was seen a few days later in a coffee shop outside in ten guys he's unmolested and he remains -- molested.
The big guys -- today I think there's a lot of explaining.
And the administration clearly wants to pretend it's political it's not it's -- it's to seek out what actually happened.
The -- thing that this potentially does.
In the context of this hearing on Brennan is open up the door to.
Al-Qaeda the strength of al-Qaeda in the region.
The -- That perhaps it's a lot stronger than.
The administration forecasts that it was.
And questions about the administration policy going after cut.
-- than it did in in courage in a back -- -- in any case some of the events of the Arab Spring which have I think by any.
Calculation resulted in a lot more chaos and a lot more uncontrollable.
Anti American -- American activity whose consequences or pursue one is just it's a much -- -- region than it was when the president.
Took office is not of course entirely his fault but the administration policy played -- -- and I think he will have to answer for that.
News and seeing as Charles is right when this first happened the president said we're gonna track them down and we're gonna.
You know apprehend them and everything and then on his interviewer -- to -- wouldn't Meet the Press he he really backed away and said well I really can't comment on these ongoing you know.
Procedures were doing we can and he he just Richard he wouldn't be specific about it I think someone needs to ask why that is -- -- -- wider investigation.
With a different.
Set of circumstances they need to be clear about that but there they don't they haven't come up with anyone any -- he says it's still a secret.
And there's -- We all know at this table there's a lot about and -- that we just don't know there are a lot of -- unanswered questions one of the -- senator Graham.
Where there's survivors and Ben -- who can tell us anything about what was going on -- -- And what happens is you give it.
To the FBI as we used to do before nine elevenths and we treated like a burglary -- -- you know in the embassy.
And then once you do that and you say it's law enforcement.
All kinds of the rules privacy procedure which preclude the administration explaining what had happened.
It was not a -- it was not a crime it was an act of terror we have to treated is that the way that we have after nine elevenths and this is a retreat to -- Nine elevenths thinking that's the reason that hey we haven't.
Gotten anywhere and.
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Biochemistry (Moscow)
The monthly journal Biochemistry (Moscow) is the international English edition of the Russian scientific research journal Biokhimiya. Biokhimiya was founded by the Russian Academy of Sciences (formerly Academy of Sciences of the USSR) and the Russian Biochemical Society in 1936. Since 1956 Biokhimiya has been followed by its English translation. The Russian and English editions are now issued simultaneously. The journal includes research papers in all fields of biochemistry as well as biochemical aspects of molecular biology, bioorganic chemistry, microbiology, immunology, physiology, and biomedical sciences. Coverage also extends to new experimental methods in biochemistry, theoretical contributions of biochemical importance, reviews of contemporary biochemical topics, and mini-reviews (News in Biochemistry). This journal is intended for scientists, postgraduates, teachers at universities and high schools, students, and specialists at research institutes. It is also a valuable resource, providing regular and essential information for libraries of university research departments, biotechnology, and biomedical departments.
14 Volumes 163 Issues 2,522 Articles available from 2000 - 2013
Browse Volumes & IssuesLatest Articles
Bacterial and cell-free production of APP671-726 containing amyloid precursor protein transmembrane and metal-binding domains
O. V. Bocharova, A. S. Urban, K. D. Nadezhdin, E. V. Bocharov… (November 2013)
Experimental and theoretical studies of mechanical unfolding of different proteins
A. V. Glyakina, N. K. Balabaev, O. V. Galzitskaya (November 2013)
Mitochondrial models of pathologies with oxidative stress. Efficiency of alkalization to reduce mitochondrial damage
N. I. Fedotcheva, E. N. Mokhova (November 2013)
- Journal Title
- Biochemistry (Moscow)
- Coverage
- Volume 65 / 2000 - Volume 78 / 2013
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- 1608-3040
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- Springer US
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If you are unlucky enough to be involved in a dental emergency, there is bound to be some delay until you can get to an emergency dentist. If you are involved in an accident or have a tooth or teeth knocked out in a sporting event, there may be little you can do for yourself. The best thing is to rely on those around you to administer first aid and medical help, and to get you to an emergency dentist or A&E department as soon as possible.
If you have a lesser dental emergency, but still require an emergency dentist, there are plenty of steps that you can take to make yourself feel more comfortable during the time you need to wait.
You don't have to have an accident to break a tooth, you can do it by eating something hard, or it happens when you chew harder than usual. A simple chip does not really require a visit to an emergency dentist as the damage usually only extends as far as the surface layer of tooth enamel. It is unlikely to cause you any pain beneath your tooth, as the nerve is still well protected. Until you can see your usual dentist (and you should make an appointment without delay) you just need to be careful about biting your tongue or your lip if the edge of the break is sharp.
The cusp of one of your back teeth can break away when you bite or chew. This is a little more serious than a chipped tooth, but it is still not really a reason for a visit to an emergency dentist. Again, you can wait for an appointment with your own dentist, which you should arrange as soon as possible. A broken cusp will leave your tooth exposed to getting trapped food caught within it, which could set up an infection and could leave you in need of an emergency dentist. While you wait for dental treatment, keep the broken tooth as clean as possible by brushing gently after eating anything.
A split, broken or badly cracked tooth that results from a knock or a fall may be more serious and you could find an emergency dentist will consider you an emergency case. The thing to look out for is whether the inner pulp of the tooth has been damaged. If it has, you are more likely to be in pain, and any delay in contacting an emergency dentist could leave you open to infection.
While waiting to see your emergency dentist, you need to help yourself to cope with the pain. Take over-the-counter pain relief: paracetamol, paracetamol with codeine, or ibuprofen are all effective pain killers. You just need to ensure you take the correct dose over the correct time period.
If a large section of your tooth has broken away in one piece, and you have to wait more than 24 hours before you can see an emergency dentist, it may be worth using some dental cement from an emergency dental repair kit to fix the broken part back again. Never be tempted to use any other type of glue. Covering the raw, exposed part of the tooth pulp should make you feel a lot more comfortable, and will allow you to take some food and drink.
If you have an obvious infection under one of your teeth, and you think you might have developed an abscess, you need to see an emergency dentist urgently. Infections can worsen quickly, and if you experience severe pain and swelling, this is always a dental emergency. If you can speak to your emergency dentist for advice, or to your own dentist, always follow their instructions. If you cannot get hold of them for several hours, or there is another reason for a delay, you can take painkillers as described above, and keep your mouth clean using a warm water mouthwash. You can also use an ice pack or frozen peas to reduce the swelling.
You may feel ill and not that hungry, and eating may also be painful, but try to take some form of nourishment in the form of drinks; soup, fruit juice, or milk for example. Rest as much as possible and look out for any change or worsening in the swelling. If you notice that you are having trouble breathing, or the swelling is pressing on your throat and making it difficult to swallow, you need to go to an A&E department urgently rather than waiting for your emergency dentist appointment.
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Green Prescription
How you live makes a difference.
Let's start with your vehicle. If you're driving an SUV, it burns one-half to two-thirds more fuel than a regular car. As for cars, newer models can vary considerably on environmental and energy efficiency, so compare both fuel efficiency and emission controls before buying or leasing.
Technology can only help so much, though. We need to reduce the number of vehicles and the time they spend on the road. One way is to hike the price of gas. As the David Suzuki Foundation points out, in Europe, fuel costs two to three times more than it does here, and European consumption is one-third less.
This is also an urban planning issue, since the growth of suburbs has extended commuting times. So we need to halt sprawl and encourage people to live downtown or close to where they work. This means greater density, with more condo high-rises along subway and bus routes. To get people out of cars, you also need better public transit, as well as more bike lanes. Businesses need to be encouraged to provide secure bicycle racks, plus showering areas.
But in the shorter term, what can you do this summer?
- Carpool, use public transportation, walk or bike (if it's not a smoggy day). One car commuter uses as much energy as a transit rider uses in 10 years. Viewed another way, if you take public transit instead of a vehicle for a year, you can save nearly a tonne of pollutants, including carbon dioxide.
- Avoid idling. Ontario stats show 3 per cent of fuel is wasted by idling.
- Tune up your car. If we all did it on a regular basis, we could reduce Nitrogen oxides by 12 per cent and volatile organic compounds (VOCs), a key part of smog, by 30 per cent.
- Keep tires properly inflated. Each 5 per cent of under inflation in a tire translates into a 1 per cent increase in fuel consumption.
- Fill your gas tank in the evening, as this a major source of VOCs. If they combine with other gases in the sun, they create smog.
- Consider fuel efficiency when you buy a car. See www.fueleconomy.gov to check gas consumption and emissions.
- Buy a hybrid. Enjoy the sound of silence as you push the button that starts the electric-powered motor. You might qualify for Ottawa's new rebate for hybrids – up to $2,000. They're expensive, but what's the price for breathable air?
- Retire the energy-guzzling clunker. A program called Car Heaven offers a free car tow and eco-friendly recycling.
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Fall's finest in Lancaster High School fall sports garnered the blue and gold spotlight Thursday, Dec. 5 in the school's auditorium.
– Editor's note: Due to reader requests, we are reprinting this Remember When column, which was originally published in the Nov. 19, 2006, edition of The Lancaster News. The story of Jim the turkey has indeed become a Thanksgiving tradition at The Lancaster News.
I don't know if it was tradition or custom, but a couple weeks before "Big Thursday" or Thanksgiving Day, me and Daddy journeyed down to Tradesville to pick up our big ole gobbler.
It's that time of year again when we celebrate by giving to others. Gonzie's Miracle Bikes for Kids is once again soliciting your help and support in providing bicycles to less fortunate children in our community.
In 2012, with your help, Gonzie's Miracle Bikes for Kids was able to provide bikes to 47 children – 32 boys and 15 girls.
Once again, we are asking you to support our efforts as we continue with this ministry. If you would like to donate bikes (old or new), please use the following drop-off sites:
Indian Land's boys, 1-1, pulled out a last-second win to earn a split with visiting Cuthbertson of Union County, N.C., on Thursday, Dec. 5 in the Warriors' gym.
Indian Land split a three-way wrestling match with Class AA North Central and Class AAA Hartsville.
The Warriors downed North Central, 58-21, but dropped a 45-25 loss to Hartsville.
Traveling down life's highways we run into plenty of obstacles. The smart fella will always figure out a short cut for himself.
To tell the truth, ever since 1966, I have made the route to and from North Elementary School. First a daughter, then a son, a granddaughter, a grandson and Bless Pete, I have the task of escorting a great-grandson to those hallowed halls of learning.
The trouble is, the kids got smarter and grandpa got lost in the shuffle.
The North and South 7-8 soccer all-stars met Thursday, Dec. 5 to decide the 2013 Lancaster County Parks and Recreation pee wee all-star soccer champion.
The South stars opened play in the best-of-three tournament with a 4-2 win over the North at the Buford Recreation Complex field on Monday, Dec. 2.
There are many honors and responsibilities that come with being a legislator. But none is more profound than sharing a community's appreciation to the men and women who have put service above self, and made the commitment to go wherever they were sent for the purpose of protecting the freedoms that we hold dear.
Scan retiring Lancaster High School football coach Bennie McMurray's resume and something hits you like a hard-charging blitzing linebacker.
Eighty-four-year-old James Hagins is a retired Lancaster resident. He spends his days taking it easy, napping in his recliner when he feels like it. When he lost his wife of 60 years, Peggy, in September of 2012, he found an unusual outlet for his grief – quilting.
"My mother quilted, and with eight of us children, she definitely had help when she needed it," he said. "I didn't mind helping her, and sometimes I was the only one she would let help, because I did things the way that suited her."


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Santa Rosa County Health Department has been forced to cut back on water sampling as the state has cut back on its budget.
Bill Sirmans, county environmental health director, said the county would still receive federal grant money for water sampling but would no longer receive state funding.
"The state budget is being cut and there's just not enough money for everything," he said.
The state was paying for half of the weekly water sampling at seven sites through the Florida Healthy Beaches program and the federal Beaches Environmental Assessment and Coastal Health (BEACH) program was paying for the other half. However, Sirmans said the reduction in funding would only allow the health department to test five sites every other week.
For more on this story, see the Sept. 15 issue of Navarre Press or subscribe online.


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Books | Movies |
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Author Payne's Book
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Shine Annie |
"Shine Annie" is a wonderful tale of growing up in rural Georgia during the early 1960's. Annie Ruth Watson, the main character, must deal with the burgeoning Civil Rights struggle and its effect on African-Americans, as she herself is coping with growing up and the responsibilities of adulthood. Filled with memorable events to which those that lived during the time can relate, the book can double as a "primer" for those that didn't. ...
View these reviews in summary mode |
Betty Oglesby Payne Message Board Talk about the novels, new and used books that Payne has written! |


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Yves Saint Laurent Velvet skirt
Bright orange velvet skirt from Yves Saint Laurent featuring a knife pleat design to the rear and side and a thin waistband with a concealed side fastening. Please note that vintage items are not new but often between 1985 and the end of 90...´s, and therefore will always have minor imperfections, even when the items have been used and worn with love and care. read more
Invest in Hedi Slimane's eagerly anticipated debut collection for Saint Laurent with this black cotton-jersey sweater. It's the at...tention to detail that makes this design so unique - panels of butter-soft leather and a silver zip nod to the designer's luxurious rock 'n' roll vision. Shown here with: Saint Laurent bracelet, cuff, pants, boots and bag. read more
Saint Laurent's black mini skirt is cut from the softest lightest leather. Lined in silky satin-twill, the interior is just as fla...wless as the exterior. Silver hardware and a draped chain add signature edge - complete the look with ankle boots and a sweatshirt. Shown here with: Saint Laurent top, bracelet, cuff, boots and clutch.Black leather Fully lined Zip fastenings along front with draped chain 100% leather (Lamb); lining: 55% cupro, 45% cotton Specialist clean read more
Saint Laurent's impeccably tailored cady skirt is a smart classic. We love how this chic piece has been fully lined in silk to ens...ure it hangs beautifully. Wear it to work with a cashmere sweater, or channel the designer's rock 'n' roll aesthetic by teaming it with a tank and ankle boots. Shown here with: Saint Laurent top, bracelet, bracelet, boots and bag.Black cady Belt loops, button-fastening back jet pocket, back vent, fully lined Concealed zip and hook fastening at side 57% acetate, 43% viscose; lining: 100% silk Dry clean read more
Strike a signature Saint Laurent silhouette in these impeccably tailored wool-gabardine pants. Detailed with zip-fastening cuffs, ...this skinny pair will make an indispensable addition to your wardrobe. We love them with a billowing blouse and pointed pumps. Small to size. See Size and Fit tab. Shown here with: Saint Laurent top, shoes and bag read more
Saint Laurent's black blazer has perfect proportions. Cut from mid-weight wool-gabardine, it maintains a smart silhouette without ...being heavy or bulky - team it with skinny jeans and heels. Shown here with: Saint Laurent top, jeans, shoes and bag. read more
Silk georgette button front blouse with point collar and long sleeves. Box pleated tiered ruffles at placket and cuffs. Available ...in Ivory. Made in France. Silk. Dry clean. read more
Saint Laurent's wool-gabardine pants are tailored with a perfect slim-leg silhouette. Leather tuxedo stripes add a chic texture co...ntrast. Maximize the super flattering low-rise cut with a blazer and heels. Shown here with: Saint Laurent blazer, top, ring, shoes and clutch.Black wool-gabardine Leather side stripes (Lamb), belt loops, two front slant pockets, two back button-fastening welt pockets, pressed creases Concealed button, zip and hook fastenings at waist 100% wool Dry clean read more
Hedi Slimane's lightweight silk-crepe blouse is a signature Saint Laurent style. The ruffled front and cuffs reflect the designer'...s '70s inspiration this season. Wear it for evening with tailored pants and polished accessories. Shown here with: Saint Laurent jacket, bow-tie, bracelet, ring, pants, belt, shoes, bag.Ivory semi-sheer silk-crepe Ruffles at front and at button-fastening cuffs, extra long sleeves Button fastenings through front 100% silk Dry clean read more
Sheer silk v-neck long sleeve dress with leather bodice, ruffled skirt, and leather button cuff sleeves. Invisible zip at back. 32..." length from shoulder to hem. Available in Black. Made in France. Silk/leather. Specialist clean. read more
Jersey long sleeve dress with jewel neck, sequin cutout detail, and sequin-embellished button cuffs. Invisible zip at back. 32" le...ngth from shoulder to hem. Available in Black. Made in France. Acetate/viscose. Dry clean. read more


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Abortion rights supporters and opponents packed a Washington state House hearing Thursday and debated a bill that would require insurers to pay for the procedure.
Supporters call the bill the Reproductive Parity Act and say it's intended to preserve existing abortion coverage once new health insurance rules come into effect under the federal health care law.
Rep. Eileen Cody, D-West Seattle, the bill's sponsor, said the proposal is needed because the Affordable Care Act will create red tape that could tempt insurers to drop abortion from their plans.
Under Cody's measure, insurers who provide maternity care, which is required in Washington state, will also have to pay for abortions.
Starting next year, insurers will be required to collect two sets of premiums, one for abortion coverage and another for all other care.
Cody said she is unaware of any carrier contemplating dropping abortion coverage.
At least 17 states have passed laws prohibiting insurance plans available for purchase on the state health care exchanges that will be part of the federal health care law from covering abortion.
This measure would make Washington the first to do the opposite, though two federally mandated multi-state insurance plans, as well as those offered by providers claiming religious or conscience-based exemptions, would be excluded.
Elaine Rose, CEO of Planned Parenthood Votes Northwest, said that other states are considering similar legislation to Washington's but that she is not aware of such bills having been introduced.
Opponents of House Bill 1044 used the hearing to say it was wrong to compel businesses and women who oppose abortion to pay for coverage for a procedure they equate with murder.
"This bill would force me to buy something I do not believe in," said Kimmy Jones, 28, a stay-at-home mother from Rainier.
Detractors also said that an exemption for insurance providers on conscience or religious grounds was not strong or broad enough.
Supporters, for their part, told members of the House's Health and Wellness Committee that women should be able to elect to have an abortion without fearing that their insurance won't cover it.
Rose said her group wants to make sure women have access to insurance that "allows them to make the decision that's best for them and their families, with their God and their doctors."
A similar measure passed out of the Democratic-controlled House last year but did not come up for a vote in the Senate.
The measure's fate will likely be determined in the Senate again this year, where a version of the bill has 22 co-sponsors, leaving it in need of three votes.


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Bring a beautiful new look to your bedroom decor with the Isadora Twin Matelasse Coverlet (White) by Peacock Alley bedding. An airy traditional chenille damask with an exquisite graceful simplicity, the Isadora collection by Peacock Alley bedding has a clean, refined look that coordinates well with any decor style. Featuring a pristine white base ornamented with an intricate floral matelasse design, Peacock Alley Isadora bedding has a classic sophistication and understated elegance that will add a touch of timeless beauty to your bedroom.
Our mission is to provide an unparalleled online shopping experience for our customers, offering the widest selection of top-quality home products at affordable prices. Founded in 2000 in Franklin, CT. the Yankee Retail Company is an authorized retailer of many world-renowned brands; such as Croscill, Waterford Crystal, American Drew, Brownstone Furniture, Reed & Barton, Swarovski, Lenox, Laura Ashley, and Waverly.


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Welcome to our home, nestled on eleven acres along Maui's renowned Kaanapali Beach. While you're with us, it will be our privilege to show you our island, our Hawaiian Culture and what "Aloha" really feels like. In the way that only locals can.
Kaanapali Beach Hotel is more than just another hotel set amongst tropical gardens. Here, you'll experience the little touches that you'd only find in any Hawaiian home, like bedspreads of Hawaiian quilt design and tropical furnishings. And always, the warmth of a smile. Because once you're here, you're family.
To view commercial, CLICK HERE!


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This lovely bride, Jessica comes to me via her little sister, Farron, who was my dear friend all through high school. We were both the new kids on the block at the new school, so at freshman orientation we locked eyes as loners and the rest is history… and by history, I mean we got into mad mischief. Good times we had, and through it all, we spent much time hanging out in the kitchen with her older sister, Jessica. After catching up with Farron down in LA after a wedding, she told me her sister was engaged. Yeah! So fun. Jess met Loren online which is starting become the majority of many of my couples. I'm so happy for Jess. Loren is perfect for her. He is funny and calm admist her super bubbly, half-spastic personality. I'm so looking forward to their beach front wedding at The Cliffs, down in Pismo.
Jessica requested an all night time engagement session in San Francisco which I had never done before and I was sure she was made aware of before attempting it. She was gracious in saying she had no expectation, so I agreed. The small world of all this was that as I looked for some night time locations in the City online, I came across one of the other mischeif makers from high school who ended up marrying a wedding photographer. Really?! I shot her hubby an email and he ended up coming out with us that night to nail some bridge shots. Big thanks to Josh for helping out that night! We walked away with some killer shots thanks to him. We started over on the Mill Valley side of the bridge to get the Golden Gate all lit up with the city a glow in the background. Then we headed into the City for some shots. Jessica needed to stop off to adjust the hair trial that was blown to pieces on the bluffs, so we popped into a restuarant. When I called her to come across the street, she waved me into the restaurant where she had a seat all warmed up, coat off, purse hung, and a drink at the bar. Love the girl. She said, "this is so us, having drinks, out at night." So I began to capture them there before we headed out to top off the night. **I used radio poppers with two 580exii flashes. One on camera, one off. At times, just off. Check it out.


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Summary: Putative RNA methyltransferase
Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.
This is the Wikipedia entry entitled "Domain of unknown function". More...
Domain of unknown function Edit Wikipedia article
A domain of unknown function (DUF) is a protein domain that has no characterised function. These families have been collected together in the Pfam database using the prefix DUF followed by a number, with examples being DUF2992 and DUF1220. There are now over 3,000 DUF families within the Pfam database representing over 20% of known families.[1]
The DUF naming scheme was introduced by Chris Ponting, through the addition of DUF1 and DUF2 to the SMART database.[2] These two domains were found to be widely distributed in bacterial signaling proteins. Subsequently, the functions of these domains were identified and they have since been renamed as the GGDEF domain and EAL domain respectively.
Structural genomics programmes have attempted to understand the function of DUFs through structure determination. The structures of over 250 DUF families have been solved.[3] This work showed that about two thirds of DUF families had a structure similar to a previously solved one and therefore likely to be divergent members of existing protein superfamilies, whereas about one third possessed a novel protein fold.
External links[edit]
- ^ Bateman A, Coggill P, Finn RD (October 2010). "DUFs: families in search of function". Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 (Pt 10): 1148–52. doi:10.1107/S1744309110001685. PMC 2954198. PMID 20944204.
- ^ Schultz J, Milpetz F, Bork P, Ponting CP (May 1998). "SMART, a simple modular architecture research tool: identification of signaling domains". Proc. Natl. Acad. Sci. U.S.A. 95 (11): 5857–64. doi:10.1073/pnas.95.11.5857. PMC 34487. PMID 9600884.
- ^ Jaroszewski L, Li Z, Krishna SS, et al. (September 2009). "Exploration of uncharted regions of the protein universe". PLoS Biol. 7 (9): e1000205. doi:10.1371/journal.pbio.1000205. PMC 2744874. PMID 19787035.
This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.
This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.
Putative RNA methyltransferase Provide feedback
This family has a TIM barrel-like fold with a deep C-terminal trefoil knot. The arrangement of its hydrophilic and hydrophobic surfaces are opposite to that of the classic TIM barrel proteins. It is likely to bind RNA [1] and may function as a methyltransferase [2,3].
Literature references
Zarembinski TI, Kim Y, Peterson K, Christendat D, Dharamsi A, Arrowsmith CH, Edwards AM, Joachimiak A;, Proteins. 2003;50:177-183.: Deep trefoil knot implicated in RNA binding found in an archaebacterial protein. PUBMED:12486711 EPMC:12486711
Tkaczuk KL, Dunin-Horkawicz S, Purta E, Bujnicki JM; , BMC Bioinformatics. 2007;8:73.: Structural and evolutionary bioinformatics of the SPOUT superfamily of methyltransferases. PUBMED:17338813 EPMC:17338813
Grosjean H, Gaspin C, Marck C, Decatur WA, de Crecy-Lagard V;, BMC Genomics. 2008;9:470.: RNomics and Modomics in the halophilic archaea Haloferax volcanii: identification of RNA modification genes. PUBMED:18844986 EPMC:18844986
External database links
Pseudofam: | PF02598 |
SCOP: | 1k3r |
SYSTERS: | Methyltrn_RNA_3 |
This tab holds annotation information from the InterPro database.
InterPro entry IPR003750
This family has a TIM barrel-like fold with a deep C-terminal trefoil knot. The arrangement of its hydrophilic and hydrophobic surfaces are opposite to that of the classic TIM barrel proteins. It is likely to bind RNA [PUBMED:12486711], and may function as a methyltransferase [PUBMED:17338813, PUBMED:18844986].
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
Loading domain graphics...
Pfam Clan
This family is a member of clan SPOUT (CL0098), which contains the following 11 members:
DUF2122 Methyltrans_RNA Methyltrn_RNA_2 Methyltrn_RNA_3 Methyltrn_RNA_4 RNA_Me_trans SpoU_methylase SPOUT_MTase SPOUT_MTase_2 Trm56 tRNA_m1G_MTAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...
View options
We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (23) |
Full (418) |
Representative proteomes | NCBI (396) |
Meta (27) |
Jalview | ||||||||
PP/heatmap | 1 | |||||||
Pfam viewer |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key: available, not generated, — not available.
Format an alignment
Download options
We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
Seed (23) |
Full (418) |
Representative proteomes | NCBI (396) |
Meta (27) |
Raw Stockholm | ||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
External links
MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
Seed source: | COG2106 |
Previous IDs: | DUF171; |
Type: | Family |
Author: | Mian N, Bateman A, Eberhardt R |
Number in seed: | 23 |
Number in full: | 418 |
Average length of the domain: | 273.20 aa |
Average identity of full alignment: | 32 % |
Average coverage of the sequence by the domain: | 79.92 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details: |
Model length: | 291 | ||||||||||||
Family (HMM) version: | 12 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
Sunburst controls
ShowThis visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...
Tree controls
HideThe tree shows the occurrence of this domain across different species. More...
Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.
There is 1 interaction for this family. More...
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the Methyltrn_RNA_3 domain has been found. There are 2 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
Loading structure mapping...


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2012-04-29 17:00
Wrong message
By Cho Mu-hyun Millions of smartphone users complained of disruption Saturday after Kakao Talk, the country's leading instant messenger service for mobile Internet devices, was paralyzed for several hours. Kakao Talk, which boasts more than 40 million subscribers here, abruptly alerted its users around 2:50 p.m. that it needed to conduct an emergency server check-up that will continue until 4 p.m. and that its messenger and Kakao Story picture upload services will be inaccessible during that time. But it wasn't until 7 p.m. that Kakao Talk managed to go back online. The company explained that the disruption was caused by a power outage at its data center in southern Seoul, operated by information technology and systems company, LG CNS. ``Our partners are very important to the Kakao team, and we believe that the disruption was unavoidable as our partners are among the top corporations in the world," the company said in a statement. ``We will cooperate to avoid any problems happening again. We will make more money to activate power conservative data centers in each continent to ensure safer maintenance. "The direct reason for the disruption was due to power problems in servers located in Korea. We have never had this problem before as there are usually two to three safety devices on our systems. We will look into the precise nature of the power problem with our partner companies." Kakao Talk claimed that the long delay in recuperating the server was unavoidable as thousands of server stations had to be checked. Finding the cause, returning power, reactivating the server and programs supposedly caused the additional 50 minute delay after power returned to the center, according to a company official. Rivals clearly benefited from Kakao Talk's troubles as data from Apple's App Store, the content platform for iPhones and iPads, show that the downloads of other messenger services like Line, T-Tok'' and My People spiked. Kakao Talk started in March 2010, and reached 10 million subscribers as of April 1, 2011. The messenger service provider has 44 million users globally, 10 million of which are non-Koreans. |


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Tag Archives: Hard Reset: Extended Edition
Fri, Sep 13, 2013 | 17:45 BST
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The Humble Weekly sale this week is full of Retro Shooters. If you pay $6 or more you can grab the following games: Serious Sam HD: The First Encounter, Serious Sam HD: The Second Encounter, Shadow Warrior Classic Redux, Duke Nukem 3D Megaton Edition, Hard Reset Extended Edition (plus soundtrack), and System Shock 2 (plus soundtrack). Head onward through the link for more information.
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The Blazers shot over 70 percent from behind the arc en route to a 130-98 win over the Jazz.
Use a Facebook account to add a comment, subject to Facebook's Terms of Service and Privacy Policy. Your Facebook name, photo & other personal information you make public on Facebook will appear with your comment, and may be used on ESPN's media platforms. Learn more.


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Quick Facts
Best Known For
Marvin Gaye was a soul singer-songwriter with Motown in the 1960s and 1970s. He produced his own records and often addressed controversial themes.
Videos see all videos
Marvin Gaye - Mini Biography (2:44)
Marvin Gaye - Mini Biography
Marvin Gaye was a soul singer-songwriter with Motown in the 1960s and 1970s. He produced his own records and often addressed controversial themes.
Smokey Robinson - Writing for Marvin
Smokey Robinson on songwriting for Marvin Gaye and how the right singer can make or break a song.
Think you know about Biography?
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Play NowMarvin Gaye. (2013). The Biography Channel website. Retrieved 07:53, Dec 12, 2013, from http://www.biography.com/people/marvin-gaye-9307988.
Marvin Gaye. [Internet]. 2013. The Biography Channel website. Available from: http://www.biography.com/people/marvin-gaye-9307988 [Accessed 12 Dec 2013].
"Marvin Gaye." 2013. The Biography Channel website. Dec 12 2013, 07:53 http://www.biography.com/people/marvin-gaye-9307988.
"Marvin Gaye," The Biography Channel website, 2013, http://www.biography.com/people/marvin-gaye-9307988 [accessed Dec 12, 2013].
"Marvin Gaye," The Biography Channel website, http://www.biography.com/people/marvin-gaye-9307988 (accessed Dec 12, 2013).
Marvin Gaye [Internet]. The Biography Channel website; 2013 [cited 2013 Dec 12] Available from: http://www.biography.com/people/marvin-gaye-9307988.
Marvin Gaye, http://www.biography.com/people/marvin-gaye-9307988 (last visited Dec 12, 2013).
Marvin Gaye. The Biography Channel website. 2013. Available at: http://www.biography.com/people/marvin-gaye-9307988. Accessed Dec 12, 2013.
Marvin Gaye was born on April 2, 1939, in Washington, D.C. He sang in his father's church and in the Moonglows before signing with Motown. He recorded songs by Smokey Robinson before becoming his own producer on the protest album What's Going On. Gaye's later records developed his production style and yielded several hits. Gaye was killed in 1984 during a domestic dispute with his father.
"War is not the answer, because only love can conquer hate."
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"Marvin Gaye is one of the greatest male voices of all time. So covering a Marvin Gaye song, especially one as quintessential as 'What's Going On,' I was a little hesitant in doing so. But I felt that it was one of those songs which spoke to a whole generation."
Early Life
Singer Marvin Gaye, also known as the "Prince of Soul," was born Marvin Pentz Gay Jr. (he added the "e" to his last name alter in life) in Washington, D.C., on April 2, 1939. Gaye was raised under the strict control of his father, Reverend Marvin Gay Sr., the minister at a local church, against a bleak backdrop of widespread violence in his neighborhood.
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Motown Records
Gaye's first certified hit under his own name wouldn't come until 1962, but his early years at Motown were full of behind-the-scenes successes. He was a session drummer for Motown legends such as Little Stevie Wonder, The Supremes, The Marvelettes and Martha and the Vandellas. Showing his stripes as Motown's renaissance man, Gaye went on to break into the Top 40 for the first time on his own in 1962 with his solo single "Hitch Hike."
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For three high-flying years, Gaye and Tammi Terrell wowed the country with their soaring duet performances of songs like "Ain't No Mountain High Enough" and "If I Could Build My Whole World Around You". Unfortunately, their reign as the Royal Couple of R&B ended when Terrell succumbed to a brain tumor in 1970. His beloved partner's death ushered in a dark period for the singer, who swore never to partner with another female vocalist and threatened to abandon the stage for good.
Political Message
In 1970, inspired by escalating violence and political unrest over the Vietnam War, Gaye wrote the landmark song "What's Going On." Despite clashes with Motown over the song's creative direction, the single was released in 1971 and became an instant smash. Its success prompted Gaye to take even more risks, both musically and politically.
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Tony Parker In San Antonio, Estate of the Day
Eva Longoria Parker and Tony Parker have been working on a huge San Antonio mansion for a while. It must be close to completion because according to the Wall Street Journal's Private Properties, the pair have listed Parker's previous San Antonio home for sale. The five-bedroom stucco house is a comparatively modest home on .65 acres that include a pool and a half basketball court featuring the logo of Parker's team, the Spurs. The home is in a gated community and it has tile floors, a large kitchen and a master suite that has a double sided fireplace that opens to a whirlpool tub in the master bath. Other spaces include a wood-paneled study and a media room that leads to an outdoor terrace. Parker bought this home for $782,040 at the age of 19 in 2001 and added the pool and basketball court. This home is listed at $895,777.
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This can be made ahead up to the point where the dish goes into the oven; just cover with plastic wrap and refrigerate. Bring to room temperature before final cooking.
5 (10-ounce) packages frozen chopped spinach, defrosted
4 tablespoons unsalted butter, dairy- free butter alternative or olive oil
2 large white onions, chopped
¼ teaspoon freshly grated nutmeg
1 tablespoon coarse salt or to taste
1 teaspoon freshly grated black pepper
2 tablespoons cornstarch or tapioca starch/flour
2 cups unflavored cold milk of choice, divided
1 cup heavy cream or milk of choice
1 cup freshly grated Parmesan cheese or dairy-free alternative*
1 cup grated Gruyere cheese or dairy- free alternative*
1. Preheat oven to 425° F. Lightly grease a 9x13-inch (or similar size) baking dish with cooking spray.
2. Place the spinach in a colander and squeeze out the liquid.
3. Melt butter in a heavy-bottomed sauté pan over medium heat. Add onions and sauté until translucent but not browned, about 15 minutes. Add the nutmeg, salt and pepper. Cook for about 2 more minutes.
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5. Combine Parmesan and Gruyere cheeses.
6. Add rest of milk and heavy cream to onion mixture and cook until hot, about 5 minutes. Add cornstarch and milk mixture and cook until sauce has thickened. Add spinach and half the cheese mixture and mix well.
7. Transfer the spinach to prepared baking dish. Top with remaining cheeses.
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SKILL LEVEL: Easy + (Level 3)
SIZE: One Size Finished Circumference 21½ in. (55 cm), unstretched. Beanie will stretch to fit a range of sizes. CORRECTIONS: None
17 sts + 24 rows=4 in. [10cm] in Stockinette st worked in the round (knit all rnds). BE SURE TO CHECK YOUR GAUGE. When you match the gauge in a pattern, your project will be the size specified in the pattern and the materials specified in the pattern will be sufficient. If it takes you fewer stitches and rows to make a 4 in. [10 cm] square, try using a smaller size hook or needles; if more stitches and rows, try a larger size hook or needles.
K2, p2 Ribbing (worked in the round) Rnd 1: *K2, p2; rep from * around. Rnd 2: K the knit sts and p the purl sts. Rep Rnd 2 for k2, p2 Ribbing
Begin Grunge Lace Pattern Rnds 1 and 3: *K2, p2; rep from * around. Rnd 2: *Yo, k2tog, p2; rep from * around. Rnd 4: *k2tog, yo, p2; rep from * around. Rep last 4 rnds 3 more times, then work 6 rnds of k2, p2 Ribbing. Bind off.
FINISHING Weave in ends.
Learn to Knit instructions: http://www.lionbrand.com/learnToKnit.html


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Go out to the terrace to enjoy views of the city and valley between Atlas ridges. The rooms were clean and spacious. Restaurant left much to be desired, but at least nobody became sick. If you prefer firm beds, like me, this is certainly the place for you. This being said, other than the view, there is nothing outstanding about... More
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Joan of Arcadia
It's been awhile since I watched this show and I never quite finished it. Now I have been rewatching and making up for the lost series and I gotta it's really good. Very surprising considering it's theme. Most shows handle an issue like God in a horrible
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NBA: San Antonio 109, Milwaukee 77
NBA: San Antonio 116, Toronto 103
COL BKB: UCLA 89, UC-Santa Barbara 76
NBA: San Antonio 102, Atlanta 100
NBA: Houston 112, San Antonio 106
NBA: Oklahoma City 94, San Antonio 88
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COL BKB: UCLA 106, Chattanooga 65
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NBA: San Antonio 92, Washington 79
NBA: San Antonio 109, Philadelphia 85
William Anthony "Tony" Parker (born 17 May 1982) is a French professional basketball player who currently plays for the NBA's San Antonio Spurs and France's national team.
The son of a professional basketball player, Parker played for two years in the French basketball league before entering the 2001 NBA Draft. He was selected by the San Antonio Spurs with the 28th overall pick in the draft and quickly became their starting point guard, helping the Spurs win three NBA Championships in 2003, 2005 and 2007. Employing his pace and high field goal percentage to great effect, Parker has been selected as an NBA All-Star three times, an All-NBA Third Team member once, and was the 2007 NBA Finals MVP.
Parker is also a music artist with his own music album TP. He married actress Eva Longoria, best known for her role in the ABC television series Desperate Housewives, on 7 July 2007. In November 2010, both parties filed for divorce.