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HOT POCKETS | Jellied stock in the filling liquefies during steaming. Justin Walker for The Wall Street Journal, Food Styling by Karen Evans, Prop Styling by DSM
XIAO LONG BAO, at their best, are works of dumpling perfection. You use your chopsticks to take one by its twirled neck and dip the fragile bundle into a mix of dark Chinkiang vinegar, soy sauce and ginger before lowering it into the cradle of your china spoon. Pierce the dumpling with a chopstick, and out rushes a flush of savory soup. You sip this elixir before biting through the skin into a delicate pork stuffing. And then, in all likelihood, a dreamy look comes into your eyes.
Finer, and trickier to make, than the boiled jiaozi dumplings people in northern China will be eating this weekend to mark the New Year, xiao long bao make an equally delightful festive treat. One of Shanghai's most beloved snacks—the name translates literally as "small bamboo-steamer bun"—it is said to have originated in Nanxiang, a county town on the fringes of the city.
How to Shape a Dumpling
Joe McKendry for The Wall Street Journal
Roll out a thin 4-inch circle of dough, rolling from edge to center and rotating so that center is slightly thicker.
Joe McKendry for The Wall Street Journal
Place 1½ tablespoons pork stuffing in center of dumpling skin and flatten stuffing slightly.
Joe McKendry for The Wall Street Journal
Pinch a series of pleats around outer rim of dumpling skin, rotating as you go.
Joe McKendry for The Wall Street Journal
Twist top of dumpling to seal.
These days, the most celebrated purveyor is a Taiwanese restaurant company, Din Tai Fung, with branches in Taipei, Shanghai and California. Their xiao long bao have thin skins, exactly 18 pleats apiece; the chefs use electronic scales to make sure each dumpling weighs exactly 21 grams before steaming.
There are other excellent iterations, like those at Shanghai's Fuchun. Lighter than the kind served out in Nanxiang, they contain stock that is rich, almost buttery, and you can order versions in which the pork stuffing is enhanced with shrimp or crabmeat.
Determined to master xiao long bao at home, I quizzed dumpling makers in Shanghai and Nanxiang, checked my notes on visits to the Taipei headquarters of Din Tai Fung and rifled through a multitude of cookbooks. The chef at Fuchun was even kind enough to give me a few tips. Maybe, just maybe, I could make something resembling his dumplings without a three-month apprenticeship and electronic scales.
The first challenge was preparing the jelly that is the key to the xiao long bao's soupy center. Made with collagen-rich pig skin, it is solid at low temperatures but melts into liquid when the dumpling is steamed. I initially tried cooking the pig skin in water, but I soon switched to a pork-and-chicken stock to achieve the depth of flavor I desired. I knew the wrappers, too, had to be just so: Too thick, and my dumplings would be stodgy; too thin, and they would rupture, losing all that beautiful stock.
I made a few different doughs, and, though all-purpose flour produced a decent result, the incontestable champion was one made with medium-gluten Chinese dumpling flour. I rolled it out on a lightly floured wooden board and pinched the wrappers around generous mounds of juicy pork stuffing laced with jelly. Then I steamed the dumplings vigorously before serving them with the traditional dipping sauce.
My friends devoured them, and my own feeling of satisfaction was incredible. Still, I reckon I'll need a bit more practice before Fuchun, let alone Din Tai Fung, will take me on as an apprentice.
Shanghai Soup Dumplings
Active Time: 1¼ hours Total Time: 1 day Makes: 25-30 dumplings
For the jellied stock:
1 pound pork ribs
1 chicken carcass
2 ounces ginger
3 scallions, white parts only
2 tablespoons Shaoxing rice wine
1 pound pig skin
1/8 teaspoon white pepper, plus more, to taste
Salt, to taste
For the filling:
1 ounce ginger
2 scallions
½ pound ground pork
1 tablespoon Shaoxing rice wine
1 tablespoon light soy sauce
2 teaspoons white sugar
¾ teaspoon salt
¼ teaspoon white pepper
1 teaspoon toasted sesame oil
For the wrappers:
1½ cups plus 2 tablespoons Chinese dumpling flour or all-purpose flour, plus extra for dusting
For steaming:
1 large head Napa cabbage
Vegetable oil for brushing
For the dipping sauce:
¼ ounce ginger, peeled and sliced into fine slivers
Chinkiang vinegar
Light soy sauce
Slow Food Fast
- Danny Bowien's Clam Omelet With Cabbage-Peanut Slaw
- Michael Anthony's Sunchoke Soup With Apples and Lentils
- April Bloomfield's Hearty Root Vegetable Soup
- Marc Vetri's Spaghetti in Parchment With Clams
- Eric Ripert's Artichoke, Fennel and Arugula Salad
- Amaryll Schwertner's Dal With Poached Eggs, Cilantro and Yogurt
What To Do
1. A day in advance, make jellied stock: Place pork ribs and chicken carcass in a large pot, cover with water and bring to a boil. Boil 1-2 minutes, then drain. Rinse well. Cover with 10½ cups water and return to a boil. Use side of a cleaver to smash a 1½-ounce piece of ginger and 2 scallions to loosen fibers, then add them to pot along with 1 tablespoon Shaoxing wine. Lower heat and simmer gently 2 hours. Remove pot from heat and strain stock. Discard chicken carcass and reserve ribs for another use.
2. Place pig skin in a pot, cover with water and bring to a boil. Boil 2 minutes, then drain. When skin is cool enough to handle, lay it on a cutting board with fat facing up and use a sharp knife to cut away and discard as much fat as possible, along with any fragments of meat.
3. Rinse pot. Return skin to pot, cover with 6 1/3 cups stock and bring to a boil. Smash remaining ginger and scallion with cleaver and add to pot, along with remaining Shaoxing wine and white pepper. Cover pot halfway and simmer gently 1½ hours. Remove skin, ginger and scallion with slotted spoon. Discard ginger and scallion, and finely chop skin. Return skin to pot, return to boil and simmer 2 minutes. Season with white pepper and salt. Strain contents of pot and discard skin. Let cool. Keep in refrigerator overnight to set jelly.
4. Make filling: Smash ginger and scallions with side of cleaver. Cover with ½ cup cold water and use your hand to squeeze vegetables to release their fragrance into water. Strain, discarding vegetables and reserving water.
5. Place ground pork in a mixing bowl. Add Shaoxing wine, soy sauce, sugar, salt and white pepper, and mix well. Stirring paste in one direction, drizzle in ginger-scallion water. Continue to stir until paste has a gluey consistency. Stir in sesame oil.
6. Finely chop jellied pork stock, add to pork mixture and mix well, stirring in one direction. Refrigerate until needed.
7. Make dumpling wrappers: Place flour in a mixing bowl and make a well in center. Pour ½ cup cold water into well and incorporate into flour. Add more water if necessary to make a smooth and pliable but fairly stiff dough. Roll dough into a ball and let rest in a bowl covered with a wet cloth for ½ hour.
8. Lightly dust a work surface and a rolling pin with flour. Break off half dough and use your hands to make a long roll about ¾-inch thick. Break off cherry-size pieces of dough from roll. Dust hands with flour and use palm to flatten each piece of dough into a disc. Use rolling pin to roll dough into a thin 4-inch circle, rolling from edge to center and rotating so that center is slightly thicker.
9. Prepare steamer: Place cabbage leaves in base of steamer and brush leaves lightly with oil.
10. Wrap dumplings: Lay a dumpling skin across palm of one hand. Place 1½ tablespoons pork stuffing in center of dumpling skin and flatten stuffing slightly. Use your free hand to pinch pleats around outer rim of dumpling skin, turning as you go. Twist top of dumpling to seal. Place dumpling in prepared steamer. Repeat with remaining dumpling skins and filling.
11. Steam dumplings: Fill a wok halfway with water and bring to boil. Stack steamer layers, cover and place in wok. Steam over high heat 5 minutes.
12. To serve: Bring steamer to table along with slivered ginger and bottles of Chinkiang vinegar and soy sauce. Set a dipping dish, china spoon and chopsticks for each guest. Let guests mix their own dips of vinegar, soy sauce and ginger. To eat, use chopsticks to lift neck of dumpling, dip in sauce and place in china spoon. Pierce dumpling with a chopstick so stock flows out into spoon. Sip soup and bite into dumpling.
—Ms. Dunlop's latest book, "Every Grain of Rice: Simple Chinese Home Cooking," is out this week from W. W. Norton.


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Male Last 20 Scores: N/H Trend Handicap 83.3 scoring avg. 62.8% Fairway 1.77 Putts 42.4% GIR Overall: 40 scores entered Courses Friends |
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Equipment falcon50driver says:
Thanks for the advice to work on putting . I think it's finally coming around 9/9/08

subset: commoncrawl


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12/15 EDIT: Current figure focus - Evil-Lyn - regular version (non Battleground). I need just the body (actually, only the tunic, boots and crotch piece to use for 2 different customs but will take the full fig if still cheap. And the Screech stand too if available). Willing to pay $15 shipped. I know she may be going for a little more than that, but I do see prices are fluctuating at times and I'm taking a shot that either someone wants quick (PayPal) cash, or maybe wants to hook up a BrORGer. If you have her, PM me! Maybe we can work a deal! Thanks!
Greetings all! Here is my B/S/T list. Hope you find something(s) you like!
If you are wanting to buy anything from my haves list below, make me an offer! As for shipping costs, it will be only what USPS charges me to send the item(s). I don't charge for packing/supplies/labor.
I am slowly building up my MOTUC collection so I still have a bunch of needs....
NOTE: Trading is preferred right now as my PayPal account is near empty. If you are looking for other toy lines, check the bottom of this post for a sample of what I have! PM me requests! But, If you have a fig (or figs) for sale that are too good to pass up, PM me! I may be able to figure something out! There is also a link to my FULL B/S/T website at the bottom of this post.
MOTUC WANTED: loose and complete preferred
In order of priority:BOLD items are most wanted.
Teela - regular version (non Goddess, non Battleground). - PENDING
King He-Man (don't need the comic at all)
Kobra Khan
Zoar (from Sorceress. Need just the stand with the falcon)
King Randor (EP version)
Spirit of Hordak - do NOT need the crossbow (I DO want the armband tho)
He-Ro - most common color of crystal is A-OK
Wind Raider
Palace Guards 2-pack
Battleground Teela
Sky High and Sky Sled (I don't care what Matty labeled it, WE know what it is!)
Stratos - TRU 2-pack version (willing to pay $15 shipped)
Snake Men 2-pack
Mosquitor - wish I could get one from Pixel Dan! (with a signed cardback!)
Hurricane Hordak
Chief Carnivus
Demo Man (must have Alcala head)
Dragon Blaster Skeletor - don't need comic at all
Snout Spout
Shadow Beast
King Grayskull - (for custom, don't need Orb) - PENDING
Castle Grayskull stands
TOD Sorceress
Bubble Power She-Ra - only want the buck with dress, no head, no accessories
Clamp Champ
Fighting Foe Men
Batros (mildly interested in him)
Geldor (not a priority at all)
Optikk (least priority)
MOC Weapons Rack
2013/2014 upcoming figs wanted:
Strongarm - PENDING
Two-Bad - PENDING
Glimmer - PENDING
Goat Man
Scorpia - PENDING
Battle Lion
Filmation Power Sword (from Flogg)
MOTUC cardbacks WANTED:
Thunder Punch He-Man
Battleground Evil-Lyn
King Randor (first release)
TRU Skeletor (just the Skeletor side)
TRU Mer-Man (just the Mer-Man side)
TRU Zodac (just the Zodac side)
BA He-Man
Buzz Off
King Hsss
Snake Man-At-Arms
Panthor (actually, just the bio is fine)
MOTUC parts WANTED:
Zoar (from Sorceress. Need just the stand with the falcon)
female crotch piece - (v2.0?) used my Marlena crotch to fix Goddess. Need one (any color) to help her stand up again.
Skeletor - Alcala head from Demo Man
BA Skeletor - head only
Weapons Rack/Pack/Accessories available that look good with the figs (no odd colors)
. - MAA sword and pistol (either deco)
. - Roboto parts in black
. - blue He-Man Axe, Shield and Power Sword
He-Man - Broken Arrow Toys custom sword hand (holds the power sword at an angle so He-Man can point it straight up in the air)
TOD Sorceress Orb Stand
Man-At-Arms - Duncan head
He-Man full power sword (to give to Adam and more) x3
Catra's sword
Tri-Klops' Doomseeker (with stand)
Mo-Larr (buck only - no head) - PENDING
Clamp Champ armor
Parts for custom figs needed:
Custom fig idea I may want to put together:Cy-Chop with normal hands (have the base fig now)
. Horde Prime - 2 silver hands (or custom cast ones)
. Evil-Lyn - tall staff
Custom fig idea I may want to put together: Filmation Man-At-Arms
. Eternian Palace Guard - buck (must have all green parts and upper boots/shins with ankle pins, as well as all 4 pieces of arm armor)
. Man-At-Arms - Duncan head and chest armor
. He-Man/King Grayskull/other brown footed fig like He-Man - feet
Custom fig idea I may want to put together:(have other parts already) IAT Shop Keeper
. Evil-Lyn (yellow version) - only need leotard and boots.
OTHER MOTU items wanted:
Masters of the Universe comics from STAR comics (1986) - have issue #3
collectible mini-comics that came with vintage figures and commemorative figures. (I have 4 already, but want more. Mainly the Alcala drawn ones and those where main characters were introduced like Ram-Man, Trap Jaw, Man-E-Faces, etc)
new DC comics mini-series (have #2 I think)
Golden Books MOTU:Secret of the Dragon's Egg (8x8 book)
other MOTU Golden Books (8x8)
80's MOTU film on DVD or Blu-Ray
DVDs WANTED:
He-Man season 1 - completes Castle Grayskull (have)
He-Man season 2 - completes Snake Mountain (have the DVDs, but need the cases!)
New Adventures - completes the spaceship (NEED)
She-Ra season 1 - completes the Crystal Castle (have)
She-Ra season 2 - the main picture of the fright zone tower (NEED)
200x/MYP complete set - completes the new Castle Grayskull (NEED)
If anyone can help me find these DVD sets, I'd appreciate it!
************************************************** ********************************************
MOTU Classics:
Sy-Klone - MOC (from Big Lots)
MOTU Vintage:Grading scale is the best I can figure out. I may be grading a tad bit better than they are. Ask for pics if curious.
Prince Adam - C6, no accessories, no shirt, works well
Orko - C7, only has hat
Kobra Khan - C8, no pistol, head squirter works great!
Hordak - C6, broken leg band, only has white crossbow thingy and chest armor (no cape)
Skeletor - C7, possibly DB version, no accessories, twisty punch broken, nice head (for swapping)
MOTU 200X (new designs):
Skeletor - C9.5, classic version, complete
Other stuff I can trade you:
I also have a TON of stuff from GIJOE, Star Wars, Transformers (mostly 1st movie), Marvel (Universe and Legends), and more! My COMPLETE B/S/T listings can be found at my website HERE.
DC Super Powers Flash - PENDING
WWF figures from the early 2000s. Note: I did find the 2nd blue elbow pad and I added a Intercontinental Title Belt (yellow strap. from the S3 Ult Warrior) to the bag holding this stuff. I will trade everything in the picture (plus the extra elbow pad and the title belt) for ONE of the MOTUC figures I want at the top of this post!
A-Team figures and other oddities. (CHiPs helmet, Mr. T, 1 rifle and black Cowboy hat are GONE.)
Danger Girl (Abbey Chase).


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Working Detail: New Stobhill Hospital ACAD by Reiach and Hall Architects and SKM Anthony Hunts
[WORKING DETAIL 25.06.09] Atrium roof detail
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text: A woman overcomes a tragedy in her own life by helping a teenage girl with a tragic family background in this made-for-TV drama. A disturbed woman who is unhappy with what she perceives to be the eccentric behavior of her daughter keeps her child a prisoner in her home for 16 years. A social worker who is still putting her life back together after the death of her own daughter gets wind of the case and is brought back into the world as she fights for the girl's freedom. To Live Again stars Bonnie Bedelia, Frances Sternhagen, and Annabeth Gish. ~ Mark Deming, Rovi


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Like millions of Americans, former national motocross champion Chuck Greene is gripped by the TV sensation that is Terror is Reality. Hosted by the flamboyant Tyrone King, Terror is Reality pits ordinary members of the public against an arena full of zombies with a simple challenge – kill more zombies than your opponents and stay alive with the winner collecting big money and the chance to come back and secure even greater prizes. So, what is it that has forced Chuck to come to Fortune City and risk his life in the modern day gladiatorial contest, is he trying to recapture the fame of his motocross days, does he have a reason to hate zombies, or is it simply the lure of big money?
Available exclusively via the Capcom Store, the Dead Rising 2: High Stakes Edition is the ultimate Fortune City-flavored edition of this game! The special edition includes a high end Fortune City poker set, the unique 'Psycho' skills pack DLC, an awesome Fortune City Visitor Map that unfolds to a larger size like a real tourist map, and a chance to own a giant six feet tall zombie replica!!!


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Research mentors and projects for the 2013 UNF REU Program
Check back regularly. More REU mentors will be added shortly!
Ecology of the Gopher Tortoise
My students and I have been evaluating the gopher tortoise population on the campus of UNF for over 20 years. This species is threatened throughout most of its range, mainly due to habitat loss caused by forestry practices, the Florida citrus industry, and development for houses and malls. Our campus population is fairly well protected which has allowed us to continue this long-term study. Each year I assemble a team of students, mostly undergraduates, who perform daily fieldwork. We begin the season by re-locating all the 400 plus burrows and assessing their activity. By mid-May tortoises begin depositing nests and we do our best to locate as many as we can. We equip nests with nest protectors to prevent predation by raccoons, then we monitor them daily until they hatch (usually mid-August to September). We also capture, measure, weigh, and mark (if necessary) as many tortoises as we can each year. This allows us to monitor demographic changes over time. This year we will also be experimenting with the use of a robotic camera that we will send into burrows to determine occupancy. We have successfully built and used a version of this camera before, and now we are trying to make it smaller so it will fit into juvenile burrows as well as those of adults.
Ecology and Biology of Sharks and Rays in north Florida waters
Dr. Jim Gelsleichter, Shark Biology, Physiology, and Ecotoxicology
My research focuses on the ecology of sharks and rays in northeast Florida waters. I also conduct research on the physiological factors that regulate shark reproduction, particularly hormones, and the effects that environmental pollution can have on shark reproduction and overall health. Undergraduates that conduct research in my program participate in the UNF Shark Biology Program's annual field survey, which examines the species composition, abundance, distribution, and movement patterns of shark and ray populations in northeast Florida waters. The goal of this research is to identify areas that are in need of special consideration and, in some cases, protection because they serve as essential habitat for ecologically and economically important and federally regulated shark species. Students also conduct laboratory research geared towards identifying the roles of hormones in shark reproduction and the effects of pollutant exposure on sharks residing in contaminated habitats including areas of the Gulf coast impacted by the Deepwater Horizon Oil Spill.
Behavioral Ecology of Estuarine Bottlenose Dolphins
Dr. Quincy Gibson, Marine Mammal Behavioral Ecology and Social Development
My lab conducts weekly boat-based photo-identification and behavioral surveys of bottlenose dolphins in the St. Johns River and is currently addressing a number of research questions related to population dynamics and behavioral complexity. Our data indicate that the St. Johns River provides important year-round habitat for estuarine bottlenose dolphins. However, given the river's metropolitan location, these resident dolphins are at a high risk of anthropogenic disturbance. Throughout our study, a number of individual dolphins have developed skin lesions, which can be an indication of disease and may be related to anthropogenic factors. Long-term photographic documentation of these lesions can be used as a non-invasive tool to assess the health status of the population. REU students working in my lab will conduct photographic analysis of skin lesions on wild bottlenose dolphins. This will involve categorizing skin lesions by type and severity, and examining potential effects of season, location, and residency patterns on lesion prevalence. Students will spend at least one full day per week assisting with data collection out on our research vessel on the St. Johns River. The remaining time will be spent processing and analyzing data in a computer lab.
Evolutionary Genetics, Speciation, Invasive Species Biology
Dr. Matt Gilg, Evolutionary Genetics, Speciation, Invasive Species Biology
Students working with me will have the opportunity to work on one of two research projects:
Selection on the gamete recognition protein M7 lysin
The first involves studying the evolution of barriers to reproduction between two sub-species of mussels (Mytilus edulis and M. galloprovincialis) from the Atlantic coast of Europe. I am interested in how proteins involved in fertilization diverge and how this divergence affects the ability of males of one species fertilize the eggs of a second species. A student working on this project will use molecular genetic techniques such as PCR and restriction enzyme digests to analyze a gene involved in fertilization called M7 lysin. Samples of recently settled mussel larvae will be analyzed to test for genetic differences among distinct cohorts of larvae.
Human mediated mechanisms of dispersal of the invasive green mussel
The second research project focuses on a local invasive species of mussel (the green mussel, Perna viridis) that is native to the Indo-Pacific. I am interested in factors that may affect the spread of this species to other parts of the United States. One possibility is that dredging equipment used by the U.S. Army Corps of Engineers may act to spread invasive marine species such as the green mussel when it is moved from one location to another. It is also possible that spoil islands that are made from the relocation of dredged material are hot spots for the establishment of invasive species allowing them to increase in population size and spread. Students working on this project will be conducting field surveys of spoil islands of various ages and dredging equipment in the local area to determine the likelihood of these contributing to the spread of green mussels and other invasive species.
Reproductive Ecology and Impact of Invasive Species
Dr. Eric Johnson, Population dynamics and quantitative fisheries ecology
Students working in my research program will have the opportunity to work on one of two research projects:
Reproductive ecology of the blue crab in the St. Johns River: A comparative approach:
Charles Darwin observed that males of many species contributed little to the sexual partnership; in other words sperm were cheap and eggs were expensive. This basic evolutionary concept has persisted through time and is the dominant paradigm in modern fisheries management where the overwhelming goal is the protection of mature females and the potential effect of males on reproductive success is ignored. However, in numerous species, the ability of females to fertilize their eggs is limited by the amount and quality of sperm that is contributed by males during mating. In particular, the unique characteristics of blue crab life history and the intense fisheries for this species make them an ideal model system for testing hypotheses related to sperm limitation. The overall goal of the project that a REU student will participate in is to fill key data gaps on basic blue crab reproductive ecology for the St. Johns River and compare the results to ongoing studies in Chesapeake Bay, a system with very different biological, environmental, and human forcing factors. Specifically, the results will provide important biological data necessary for effective management of blue crabs in the St. Johns River, and generate a strong comparative data set for ongoing tests of sperm limitation in Chesapeake Bay.
Food web impacts of invasive lionfish in nearshore coastal ecosystems of Florida
Introductions of invasive species pose a serious threat to native ecosystems worldwide. Once established, invasives can precipitate far reaching and often unpredictable effects in native coastal ecosystems through predation, competition for prey resources or habitat, and can ultimately lead to declines in the abundance, diversity or extinction of native biota. Two closely related species of lionfish native to the Indo-Pacific were introduced to Florida's east coast and have expanded rapidly throughout the marine waters of the western Atlantic Ocean and Caribbean Sea. Although now common on marine coral reefs, lionfish have only recently been observed within estuaries, in particular residing in mangrove and seagrass ecosystems, with unknown, but potentially substantial detrimental effects for many economically important commercial and recreational fishes that utilize these habitats as juvenile nurseries. In a second project that REU students can participate in, I propose to investigate the food web impacts of invasive lionfish in Florida estuaries with particular focus on these recently invaded estuarine ecosystems using both traditional and novel approaches. This research will enable fishery biologists to more effectively manage this established species, help predict the impact of lionfish on economically important native fishes, and provide key data for ecosystem-based fisheries management which relies critically on knowledge about trophic dynamics and food web structure.
Salt Marsh Restoration
Drs. Matt Kimball and Kelly Smith, Fish Ecology
Comparing different living shoreline methods for salt marsh restoration within the Guana Tolomato Matanzas National Estuarine Research Reserve (GTMNERR).
The GTMNERR has several areas where marshes (shorelines) are experiencing significant erosion. The long term goal of the project that a REU student will participate in is to reduce erosion and salt marsh loss in an area utilized by a number of important recreational and commercial fishery species. A previously funded project established nearshore oyster reefs to promote shoreline growth. The new project (expected to begin in May of 2013) will add additional treatments to reduce erosion using fiber logs embedded with ribbed mussels. These structures should accelerate shoreline accretion as well as provide habitat structure for coastal fishes. Students would assist in pre-restoration monitoring of coastal fauna (primarily fishes and invertebrates), restoration implementation, and some post-restoration monitoring of habitat use by coastal fauna. Side projects that students could develop under advisement from the co-mentors include: comparative settlement patterns of benthic fauna with shoreline treatment, population structure of resident salt marsh and oyster reef fishes in the restoration area, comparative growth rates of salt marsh cord grass with shoreline treatment. This is a field-based project so students will need to be comfortable working under rigorous field conditions. The project should be of particular interest to students interested in ecological restoration.
REU students working in my research program may be involved in one of the two following projects. In both cases, REU students will complete laboratory based projecst that may be presented at an international scientific meeting. Participating students will learn and become proficient with laboratory assay techniques including radioimmunoassays, western ligand blots, spectrophotometric assays and other basic molecular biology techniques.
Milk vs. Formula: Examination of Metabolic Hormone Profile in Common Pinniped Rehabilitation Formulas
Many rehabilitation facilities, zoos, and aquaria in the United States, Canada, and Europe care for injured or abandoned pinniped (seal and sea lion) pups. While rehabilitation is common, success rates are low with 50-70% mortality. Nutrient intake is an essential component to rehabilitation success. First year survival of pinniped pups is positively correlated to body mass. In the wild, seal pups nurse on energy dense, lipid rich milk (60% lipid) that results in an average daily gain (ADG) of 5 to 9% of body mass per day. Rehabilitated pups are fed via esophageal feeding tube with formula that maximizes lipid and energy content, on the assumption that these are key parameters. However, to our knowledge no attempt has been made to evaluate and compare the endocrine contribution of natural pinniped milk with commonly used rehabilitation formulas. In contrast to formula fed pinnipeds, human infants fed formula grow at a faster rate than breast fed infants. Human formula contains several bioactive hormones in greater quantities than breast milk including increased ghrelin, and insulin-like growth factor. This study will compare metabolic hormone profiles in pinniped rehabilitation formulas with milk from free-ranging pinnipeds. These data will provide new information that could improve rehabilitation formulas. Addition of metabolic hormones to formula may improve growth rate and therefore rehabilitated pup survival.
Determining the Influence of Seasonality on the Metabolic Hormone Response to Nutritional Stress in Steller sea lions (Eumetopias jubatus)
In extreme cases, nutritional stress due to a reduction in the quantity or quality of prey consumed, may lead directly to emaciation and death, however, more subtle effects of nutritional stress may also result in decreased survival. Nutritional stress can make animals vulnerable to disease and predation which may have substantial impacts on survival and population trends. Between 1970 and 2000, Steller sea lions (Eumetopias jubatus) underwent an 80% reduction in population size and have not yet recovered. Although the cause for the population decline and slow recovery of these populations is likely multifaceted, early evidence did support the hypothesis that nutritional stress contributed to the initial population decline; however, later research has been inconclusive. Limitations of this research, include poor knowledge of the mechanisms linking nutritional stress to changes in survival and reproductive rates. Confounding our understanding of these mechanisms is the strong seasonality of normal Steller sea lion life history. Studies have suggested that seasonal physiology of Steller sea lions may have a greater impact on body condition, one metric used to identify acute nutritional stress, than changes in prey quality. Therefore the focus of this research is to evaluate the physiological mechanisms of the seasonal response of Steller sea lions to nutritional stress. Understanding the seasonality of the physiological response to nutritional stress will allow us to better understand and quantify the impact in wild populations.
Stress Responses
of Marine Organisms
Dr. Cliff Ross, Stress Responses of Marine Organisms
Pathogen-Induced Defense Responses in Seagrasses
Seagrasses are widely distributed marine vascular plants that are an integral component of coastal ecosystems. Several seagrass species are susceptible to periodic outbreaks of a "wasting disease" (presumed to be affiliated with the opportunistically pathogenic slime mold Labyrinthula sp.) that can contribute to rapid population declines. Although there is an increasing awareness of the impacts of diseases in the marine environment, the elucidation of marine plant defense responses against invading pathogens is just emerging. Fortunately, due to the value of agriculturally related plants, a tremendous amount of research has been placed on understanding the basis of active defense responses in terrestrial plants. Bearing in mind that marine angiosperms (seagrasses) are descendants of terrestrial plants, we can use this work as a platform to further our understanding of defense systems in marine plants. Using seagrass (turtle grass) and Labyrinthula sp. as a model system, we are particularly interested in the structural and functional identification of elicitors, intracellular signal transduction components, and the transcriptional activation of defense proteins and secondary metabolites. Specific projects will address: 1) how seagrasses produce secondary metabolites in response to infection; and 2) ff changing environmental conditions (e.g. changes in light, salinity, temperature) influence seagrasses susceptibility to infection.


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GPC – General Polygon Clipper library The University of Manchester GPC library
GPC Licensing GPC is free for downloading and time-unlimited evaluation by anyone. |
Non-commercial use of GPC (for example: private / hobbyist / education) |
GPC is free for non-commercial use only. |
Commercial use of GPC (for example: product development / commercial research) |
If you wish to use GPC in support of a
commercial product, |
Download GPCGPC v2.32 (zip, 28k). On-line documentation is here. |
Maxim Shemanarev, developer of the Anti-Grain Geometry
high quality rendering engine, has kindly donated
Windows application which shows GPC in action.
The gpctool package allows the interactive graphical evaluation of the GPC library under Unix / X11. Compilation requires the Mesa (or OpenGL) and xforms libraries - no precompiled binaries are available. The gpctool sources are available as either a Unix compressed tarfile (62k) or a PC Zip file (38k). Please note that this software is completely unsupported.
ActionScript 3 port | Jakub Kaniewski |
C# wrapper | Stefan Menne / Guido Bonino |
Cocoa/NSBezier wrapper | Graham Cox |
COM wrapper | Tom Wolf |
Delphi/Pascal port (GPC v2.30) | Stefan Schedel |
Delphi/Pascal port (GPC v2.32) | Richard B. Winston |
haXe port | Michael Baczynski |
Haskell port | Marco Túlio Gontijo e Silva |
Java port | Daniel Bridenbecker | Javascript port | Arif Buntaran |
Lua binding | Luiz Henrique de Figueiredo |
OCaml binding | Coherent Graphics/John Whitington |
Octave binding | Rafael Laboissiere |
Perl binding |
Eric Wilhelm |
Python bindings |
Joerg Raedler |
VB.NET wrapper | Tadej Basa |
If you are a non-commercial user and you have found GPC to be useful, we invite you to please make a donation and help support the continuation of this project. Thanks!
However, If you wish to use GPC in support of a
commercial product, you must obtain an official GPC Commercial Use Licence from
The University of Manchester.
Please email for details.
Page last updated:
25 August 2013.


text:
"But I don't think Hank done it this way, no..."
I don't think Hank Hill did things this way too, Waylon Jennings.
Introducing Trevor, everyone's favourite hillbilly drug dealer. Find out what scrapes he'll get into in Grand Theft Auto V.
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"But I don't think Hank done it this way, no..."
I don't think Hank Hill did things this way too, Waylon Jennings.
لعبة رائعة جدا ولاكن نرجو من شركت روك ستار أن يترجمو اللعبة الى العربية ترجمة فقط لانطلب شيئا صعب أرجوكم أنا أأكد لكم ستباع نسخ كثيرا جدا أذا ترجمتوها ألى العربية ألي معي يعطيني لايك ليروها المسؤليت في روك ستار A very wonderful game and I hope the game translated to Arabic translation Just do not ask something difficult I assure you very much copies will be sold if translated to Arabic ........ With me gives me the Lake to see it Contact Person in Rock Star
If it doesn´t have real vehicles, don't count me in this time. I'm tired of driving proxies in cities with unreal names. Besides, I don't want to use a psychotic piece of shit ugly bold.
i wish hairstyles could be customizable
i hated niko's hairstyle and facial hair in gta iv , lol
i have a good feeling about this game for the simple that they Turned "The Red Dead Series" into a huuuge game with a great story line. But this guy seems like your typical Psychotic White Trash of america high on methamphetamine hahahaha! Seems like he belongs on a Episode of Breaking Bad, and he looks like he'd be realated to Kane from Kane and Lynch.. except Meth'd out...
let me guess he smoked too much meth ? typical white trash white guy and poor me i live in the black ghetto and mr yuppy characters... haven't we done these already ? c'mon Rockstar lose Michael and Franklin it's played out at least Trevor is funny in a twisted burned out way...oh well I like blowing the shit out of everything then the police,then getting away ....PC users get screwed again ,damn might have to buy it.
Rockstar never fails. I hope Rockstar will not fail to bring this game on PC......
GTA IN PC ......Its AwEsOmE.This game isnot looking as good to me anymore tbh. I dont wanna play a episodic game, i like the epic feeling of playing with one guy and following their trials and tribulations all the way through. Pulp fiction was a great movie but trying to make in to a game is gonna suck. Why did they have to ruin this game by forcing us t play through the lives of all these different people. Wouldnt it make more sense if it was a choice?
Trevor looks like a freaking hilarious character. I love Rockstar's writers. Trevors last line was hilarious. Hurry and come september!
i just want to reenact the north hollywood shootout in a GTA environment with Max Payne 3 graphics and i'll be happy
The character models are starting to look dated. It would be awesome if this was released on next-gen consoles! I wouldn't mind the extra wait.
Trevor looks like an older version of Johnny Knoxville. The facial resemblance is uncanny!
Here are all together please visit this: http://www.gamingtracks.com/2013/05/gta5trailers.html
It's a no piracy site
Mike = rich lifestyle
Franklin= urban life style
Trevor = out of your mind psycho life style
just cant wait for this game
hahah i love this crazy character it seems that now it will be a more interesting story or "campaign" i wonder if some of GTA:SA character will be appear here at least like a reference or cameo
@Serpens_Solida quit gaming and starting living in the real world then... real city names, real vehicles... plus you could be a handsome psychopath and shoot other people IRL too....
@swyg lol hillbilly is not an accent.. hillbilly is the way you live.. do you know what a hillbilly is? if you mean southern accent then that would make more sense, As i do have a Southern "Cajun" twang in my voice.. lemme break it down for you.. Hillbillys Do not live in the City or in the Country. They Live in the Mountains, they Have ZZ Top Beards, They have Shat Houses, Most of them are hunters/survivalists off of the land.. But if you mean hillbilly as a sterio type meaning trashy,live in a trailer,probably touches his cousin, cant live without a bottle of whiskey every night, gets drunk and yells and screams and smokes meth.... then you mean the term "white trash".. but if you mean someone who half-owns a car garage, works hard for a living but enjoys life with a bottle of whiskey and occasionally hollers when drunk.. then thats a "Red Neck".. im not even white and i knew that lol! ;)
@Coolyfett might not even be a very good game tbh, i mean wtf we have to play as differnt people? wheres the immersion then? I mean thats the ultimate immersion breaker, ye will appeal to all the kids who have attention spans of fishes, but for the people who the game was supposed to be made for it will probably suck :S I mean GTA 4 was a worse game than SAn andereas. Vice city had more charm than San andreas though. So you can see a steady downfall in the series.
@p4kman88 That's what I always loved about GTA games. You could always in some way reenact scenes from movies, or certain things in history. I used to love reenacting the end of Scarface in Vice City, except when I'd be close to death I'd change the ending and run up to the helipad and escape
Its sucks that the game has ADHD though. I mean why do we have to play as so many different people? That is dum. It would be cool if you could choose, or make a party, but my guess is the game will end up feeling like a soap opera, at least thats the way its been described to me.
@amar1234 @Coolyfett Immersion?? Pulling an RPG from nowhere is quite the opposite but I dont see you complaining about that
@amar1234 @Coolyfett every GTA game was massively successful. To think otherwise is completely idiotic.


text:
Your source for the latest news and events regarding The Three Stooges.
Christmas Is Just Around The Corner
We look at the calendar and Christmas is coming soon!…So…Here comes the rest of the Holiday Season! Christmas is the big event coming up with virtually every household in America celebrating Christmas in one fashion or another. Chanukah started on … Read More
Last Three Stooges print has the last laugh
Posted in the In The News section
A garden shed in a northern-beaches suburb of Sydney, Australia, may sound like a remote connection to a Three Stooges film festival being held at Glendale's Alex Theatre on Nov. 30, but the rare find by a film collector there has fans of the zany comedy trio rejoicing.
The Three Stooges, a former Vaudevillian troupe, made 190 short films in their long Hollywood career. One film, the Technicolor "Hello Pop," an MGM 18-minute backstage musical made in 1933 was thought lost forever when a fire at MGM studios in 1967 destroyed what was then thought to be the last print. Read More
Thanksgiving Traditions
Posted in the Stooge Stories section
Thanksgiving tradition tells us this holiday started in 1621 with the Pilgrims at the Plymouth Colony. The celebration was to commemorate the harvest after a harsh winter with Governor William Bradford proclaiming a day of Thanksgiving. The colonists celebrated with … Read More
C3 Entertainment, Inc. Licenses an All New Live Three Stooges® Stage Show and Television Broadcast of The Three Stooges® Classic Shorts in Argentina
Posted in the Press Releases section
Ani Khachoian, C3's Executive Vice President of Licensing & Consumer Products said 'South America and Argentina specifically have millions of Three Stooges fans, and this new license will elevate the presence of The Three Stooges® brand in Argentina even more. An all new exciting live Three Stooges Stage Show, as well as broadcast of the classic The Three Stooges shorts on free television on the American TV Channel will bring the wild antics of the greatest comedy team ever to a whole new audience in South America.' Read More
The Three Stooges 16th Annual Big Screen Event
Posted in the Current EventsStooge Stories section
'Hello Pop', the lost MGM short from 1933 with Larry, Moe and Curly with Ted Healy has been found! Now restored, it will be shown as it was originally intended, in 35mm on the big screen and in two strip Technicolor! So after 80 years, once again we can say, 'Hello Pop! Glad you made it!' Read More
C3 Entertainment, Inc. Licenses Star Cutouts For The Three Stooges® Brand Cutouts & Face Masks
Posted in the Press Releases section
Ani Khachoian, C3's Executive Vice President of Licensing & Consumer Products said 'Joining with a global leader like Star Cutouts as a Three Stooges licensee gives fans around the world more quality products to choose from for themselves and as gifts. In this case, Three Stooges cutouts and face masks. The products from Star Cutouts are fun, with terrific graphics and truly lifelike. The cutouts and face masks make for a wonderful addition for every fans' collection.'. Read More
Happy Birthday Curly!
Posted in the Stooge Stories section
Curly. That single word evokes an image and brings smiles and laughter to every corner of the globe. It is instantly recognized and in the mind's eye a picture appears of one of the funniest men to ever share his talents. To paraphrase an old adage, it's a word worth a 1,000 pictures or at least the 97 Columbia shorts he made with Moe and Larry! Read More
Halloween Recipes
Posted in the Stooge Stories section
Candied Apples Ingredients 2 Cup granulated sugar 2 Cup corn syrup 1/3 Cup cinnamon candy 1 Cup water 3/4 Tsp. cinnamon 1/2 Tsp. vanilla 1/4 Tsp. cloves 3/4 Tsp. red food coloring 6 Med apples Preparation Remove stems from apples … Read More
Fright Night With The Three Stooges
Posted in the Stooge Stories section
What better way to prepare for Trick or Treat time than with Spooky Three Stooges Shorts. The Boys met up with their share of ghosts, goblins and assorted scary types so to get in the mood to go haunting on Halloween, turn out the lights and tune in these shorts for some delights and frights! Read More
Happy Birthday Larry!
Posted in the Stooge Stories section
Larry's birthday is October 5th. He was born on Philadelphia in 1902. He began his career in vaudeville and his act included playing the violin. Larry met his wife Mabel while both were performing in vaudeville as Mabel performed with her sister Loretta as the Haney Sisters. They often performed at the same venues at the same time on the various vaudeville circuits. That's Larry with Mabel and their daughter Phyllis. Read More


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Bought at Negotiated Price $182.00
(3 purchases)
Glen Burnie, MD
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Move hair comments:
1. Product name: 18"20"22" 3 pks human hair virgin Peruvian Remy hair weft weaving deep wave machine weft
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4. Weight: 95-100g/pcs.
5. Color: #1B (Natural Black
6. Texture: Body wavy. We have straight,body wavy,natural wavy,deep wavy, french curly wavy hair in stock.
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text:
+Hurst Capital plans to bid on Hostess
The local company filed a letter of intent with the U.S. Bankruptcy Court.
+City finalizes St. Armands parking changes
The city of Sarasota will be making some parking changes on St. Armands Circle, with some of the changes coming before Thanksgiving Day.
+Mowing issues continue on Siesta Key
Sarasota County could create as many as 28 temporary positions to buffer median upkeep; Canal Road right-of-ways will continue to grow until next month.
+Entertaining on Easy Street
The Out-of-Door Academy's kindergarteners performed a Thanksgiving Program Monday, Nov. 19, in the Multi-Purpose Room at Out-of-Door Academy on Siesta Key.
+THE SWEET SPOT: Unique takes on a traditional side
The Observer family shares its sweet potato traditions — sans the marshmallows.
+Popcorn Bob's Movie Magic
Enjoy this week's movie reviews by Popcorn Bob.
Currently 0 Responses
- Dec
12 La Leche League/Breastfeeding Support
10:00 am - 12:00 pm - Dec
13 Fifth Annual CYD Gingerbread Festival
10:00 am - 7:00 pm - Dec
13 CYD Gingerbread Festival
10:00 am - 6:00 pm - Dec
14 Free Spirograph Art Event For The Kids
10:00 am - 4:00 pm - Dec
14 Green Tara Empowerment
10:00 am - Dec
14 Cat Depot: Leashwalking
12:00 pm - 1:00 pm - Dec
17 Financing Our Foodshed with Carol Peppe Hewitt
6:30 pm - 8:30 pm - Dec
18 8th Annual Sustainable Communities Workshop
8:00 am - 4:30 pm
Turkey tidings
During each of the last eight years, the Out-of-Door Academy families have collected food for Gocio Elementary families. -
Forty Carrots hosts grandparents
Forty Carrots Family Center invited students' grandparents to attend Grandparents' Day Nov. 25. -
Holiday ring
The Rotary Club of Sarasota Keys is seeking volunteers from now through Dec. 24, to help man its Salvation Army kettle bell during the holiday season.


text:
There's more to life than the freakin weather. I think there's a ridiculous amount of time and energy wasted on the subject of how cold it is, how hot it is, whether it's raining, snowing, sleeting, muggy, dry, cloudy or sunny. "Hot out, isn't it?," "Sure wish the Spring would get here.", "What's the weather like today?"… There is an entire cable TV station devoted to it. The news spends about 10 minutes (or does it just feel like 10 minutes) on the weather, and the fact is, there is absolutely nothing we can do to change it. I think when I grow up (hah!) I want to be a meteorologist. You get to be on TV, you make lots of money and it doesn't matter if you are right or wrong. Sounds like a pretty deluxe deal to me. An awful lot of people even choose where they live by the weather – not the theatre, music, cuisine, art, or museums available. And some folks that I can think of go to the hottest regions of the country and then complain about how hot it is. They never go outside at all! They don't go anywhere there isn't air conditioning (and then complain about the draft). I'm tired of it, but just like the weather, I can't do a dang thing about it.
Yours in annoyance,


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SOURCE The Kroger Co.
CINCINNATI, Sept. 26, 2013 /PRNewswire/ -- The Kroger Co. (NYSE: KR) announced today that four associates have been named Certified Cheese Professionals™ by the American Cheese Society.
Kroger associates Marcella Wright (Portland, OR), Mark Lucier (Cincinnati, OH), Logan Class (Dayton, OH), and Brandon Schilling (Atlanta, GA) have joined the ranks of an elite group of 253 individuals who have passed the Certified Cheese Professional™ Exam. The exam was created by the American Cheese Society to promote the large and comprehensive world of cheeses and encourage food industry professionals to master the knowledge.
"We are proud of Marcella, Mark, Logan and Brandon for dedicating themselves to their craft and sharing their passion for cheese with our customers," said Margaret McClure, Kroger's vice president of deli/bakery. "Their designation as Certified Cheese Professionals is an inspiration to Kroger's many talented cheese stewards who take pride in delighting our customers."
With U.S. per capita cheese consumption at all-time highs and projected to grow, Kroger is committed to being the destination for cheese. Through an exclusive partnership with New York's Greenwich Village-based Murray's Cheese, the Kroger family of stores hosts more than 89 Murray's Cheese shops in stores from coast-to-coast.
For more information about ACS CCPs™, please visit www.cheesesociety.org.
Kroger, one of the world's largest retailers, employs 343,000 associates who serve customers in 2,418 supermarkets and multi-department stores in 31 states under two dozen local banner names including Kroger, City Market, Dillons, Jay C, Food 4 Less, Fred Meyer, Fry's, King Soopers, QFC, Ralphs and Smith's. The company also operates 783 convenience stores, 326 fine jewelry stores, 1,195 supermarket fuel centers and 37 food processing plants in the U.S. Recognized by Forbes as the most generous company in America, Kroger supports hunger relief, breast cancer awareness, the military and their families, and more than 30,000 schools and grassroots organizations. Kroger contributes food and funds equal to 200 million meals a year through more than 80 Feeding America food bank partners. A leader in supplier diversity, Kroger is a proud member of the Billion Dollar Roundtable and the U.S. Hispanic Chamber's Million Dollar Club.
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Home / Alex Perry
Alex Perry Instagram Gallery
Alex Perry Interview – Humble Beginnings, Career High Points, and More
Alex Perry on How to Find the Perfect Winter Coat
Alex Perry's Tips For Keeping Beige at Bay this Winter
Alex Perry MBFWA: Watch the Full Runway Show
Alex Perry Interview at Mercedes Benz Fashion Week 2013
Alex Perry on his Sydney Fashion Week 2013 Show
Alex Perry on How To Find Your Signature Style
The Oscars 2013: Alex Perry's Best and Worst Dressed
Golden Globes 2013: Alex Perry's Red Carpet Wrap
When it comes to Australian designers, you can trust Alex Perry to bring the glam like no other. With A-listers including Jennifer Lopez, Sandra Bullock, Miranda Kerr, Elle Macpherson, Megan Gale and more having worn Alex Perry dresses on the red carpet, and the Alex Perry bridal collection making waves around the world; there's no doubt Perry has mastered art of dressing for the occasion. As one of Australia's Next Top Model judges, Perry has won over Australia with his quick wit and candidness.
Here Alex Perry shares his tips and tricks for looking fabulous year round, and achieving red carpet glam worthy of an A-lister.


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- Products:
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DefinitionsHammered Satin Jumpsuit
Definitions Satin Jumpsuit with Wrap Top Detail
A futuristic rework of the retro classic
Introducing our latest exclusive brand ready for AW13, Definitions features structured cuts, tailoring and sharp edges for style driven women who want a high-end fashion finish.
Playing with textures and styles this jumpsuit by Definitions channels the season's love of mixing it up.
Sumptuous satin like fabric is soft-to-the-touch and lends this piece a luxuriously smooth texture. In black this piece has a timeless classic quality, whilst the grey adds an industrial yet futuristic appeal to it.
Wide, elbow-length sleeves create a boxy style that is softened by the detail to this piece. The wrap top creates a beautifully feminine neckline, whilst the fabric tie waistband cinches your waist for a flattering fit and the tapered legs give a more fluid silhouette.
All this jumpsuit needs is a chunky beaded necklace and a pair of pointed toe stiletto heels to raise your profile. A simple clutch bag will complete your retro style.
Whatever you do, do it in style
Useful info:
- Jumpsuit by Definitions
- Luxe satin fabric
- Wrap top detail
- Tapered leg style
- Zip to back neck
- Elbow length sleeves
- Satin waistband tie
- Weekly Price
- From £1.85 per week
- Price
- Was £74.00Now £37.00Save £37.00
- In Stock
- Next day delivery available
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SouthHalterneck Jumpsuit
Wide legs and front halter neck. Inside leg: 29 inch.
95% Viscose, 5% Elastane. Machine washable. Colour: Purple.- Weekly Price
- From £0.70 per week
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- Was £35.00Now from £14.00 to £35.00Save Up To £21.00
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- Available
DefinitionsLace Plunge Front Jumpsuit
Definitions Wide Leg Jumpsuit with Animal Lace Panel
Introducing cat couture into this season's wardrobe
A chic alternative to the classic LBD, this wide leg jumpsuit by Definitions is a statement investment piece for the season ahead.
Featuring an animal lace panel to the front this piece unleashes the feline within. Vintage-inspired the lace detail contrasts beautifully against the soft jersey fabric for a striking texture clash. The wide leg is flattering and introduces a touch of retro to your look, while cap sleeves are classic.
For an all out elegant evening look wear this jumpsuit with a statuesque pair of heels, chunky black jewellery and an animal print clutch bag. Sleek your hair back into a classy updo for a starlet finish.
Whatever you do, do it in style
Useful info:
- Jumpsuit by Definitions
- Animal lace panel
- Wide leg style
- Plunge front
- Jersey fabric
- Zip fastening to back
- Weekly Price
- From £1.10 per week
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- Low stock, selling fast
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Love LabelContrast Sleeve Playsuit
Love Label Long Sleeved Playsuit
With contrast sleeves this playsuit is a striking piece that's packed with gorgeous detail. In nude and black this playsuit lends a retro inspired style to your wardrobe this season.
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Wear this long sleeved playsuit with court shoes or platform heels. For day, pare down with simple leather flats and a tote bag.
Useful info:
- Playsuit by Love Label
- Long semi-sheer sleeves
- Banded waist with pleats
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text:
I did a little "drive-by" of the Spellbinders Idea Gallery today, and saw a few of my projects from last winter had been posted. I really love this London layout so I wanted to share it with you. I took these photos when my husband and I were in London for our anniversary in 2009. {If you are sick of my Paris and London photos, you will be happy to hear that we are celebrating our 25th anniversary this year by going to Alaska! New photos to scrap!}
I took these photos of the pubs which were around the corner from our hotel. The flowers were so pretty. Plus, I had to take a photo of the "Drury Lane" sign to show my kids...because in one of the Shrek Movies they talk about the Muffin Man who lives on Drury Lane. {It's a sad day when all of your movie quotes are from Shrek, Toy Story, Princess Bride, and Die Hard!}
I created all of the flowers with the Spiral Blossom Three die template. I cut the flowers from printed paper, and once they were rolled, I let them "relax" a bit so that I could glue a dew drop crystal into the center.
I even did a journaling spot for this page - with HANDWRITTEN journaling! I don't do much journaling, which makes it extra important when I do!
I really love how the gray and hot pink worked together for this layout. It is not my normal color palette, but it came together so nicely. Plus those flowers are do die for!
Spellbinders: Die templates: Tea Service, Nested Tea Cups, Spiral Blossom Three, Moroccan Motifs, Moroccan Accents, Grand Calibur Die Cutting System
Stamps: Gina K Designs Tea Time, Unity Stamp Company Unforgettable Moments, Heidi Swapp Date stamp
Cardstock: Bazzill Basics
Designer paper: My Mind's Eye Stella and Rose, Anna Griffin Carmen
Ink: Clearsnap Colorbox Rouge, Azurite Fluid Chalk Ink; Graphic 45 Decades Photogenic Antiquing Ink; Sharpie gray journaling pen
Embellishments: Creative Impressions brad; Robin's Nest Dew Drops clear bling; Maya Road chipboard letters
Adhesive: Scrapbook Adhesives by 3L: EZ Runner, 3D Foam Squares; glue
Wednesday, May 16, 2012
4 comments:
- contadinak said...
Love these, Gloria! SO great to see your amazing talents with layering span the crafting spectrum! And thanks for introducing me to spellbinders dies i must have!!!
- kathy/North Carolina said...
Gloria...love this...commented on FB...but I wanted to let you know that I can't open your latest blog post...get a message saying the page doesn't exist...just thought I'd let you know.
- Cultivate Life in Joy said...
so pretty..love the color combination and the paper design you chose for the flowers..so pretty..did i say that again...so pretty
- Christine said...
Gorgeous layout and yes, those flowers are stunning :)
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- Patrick B. Pexton
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Patrick Pexton was Washington Post ombudsman from March 2011-March 2013. Doug Feaver now serves as reader representative and can be reached at 202.334.7582 or firstname.lastname@example.com. Pexton came to The Post from National Journal, where he was deputy editor, the No. 2 job at the nonpartisan weekly magazine about politics and government. Before National Journal, Pexton worked for the Army Times Publishing Co., where he was an editor, chief Pentagon correspondent and an investigative reporter. He played a key role in uncovering two national stories about the U.S. Navy: the Tailhook sexual harassment scandal and the widespread cheating by midshipmen at the U.S. Naval Academy. At the beginning of his career, Pexton worked at the Shoreline Times newspapers in Southern Connecticut and the Journal newspapers in the Washington suburbs. A Los Angeles native, Pexton graduated from Claremont McKenna College and earned a master's degree from the Johns Hopkins School of Advanced International Studies (SAIS).
- Post home subscribers get price increase too
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- The Post uses someone else's words — again
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Carolyn Hax Hootenanny of Holiday Horrors
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Celebrate the season by swapping horror stories from holidays past and doling out advice for navigating difficult holiday situations.
12:00 PM | Carolyn Hax Live: Hootenanny of Holiday Horrors 2013 (Friday, Dec. 13) LIVE NOW |


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Remember Navy SEAL Adam Brown
SEAL Team Six member's remarkable story of bravery
May 29, 2012
Eric Blehm, Brian Valenza
You're watching...
SEAL Team Six member's remarkable story of bravery
May 29, 2012
Eric Blehm, Brian Valenza
Also in this playlist...
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Auto-advanceThis transcript is automatically generated
They are.
Always told where they're going are who their target is.
But Navy SEALs take on the real Mission Impossible assignments.
Navy SEALs are among the most highly trained in the united states military.
-- -- -- -- -- Ruling recruiting process that involves seen air and land training.
The program is designed to be the ultimate test of one's streak -- -- -- historic part.
-- the dropout rate for the 21 week training course it's nearly 70%.
And for those who make.
The missions they must carry out require the ultimate command of physical abilities and -- it's.
The most famous of these missions may 2011.
Navy seal team six carried out the killing of America's number one target.
Osama been on the seals -- -- landed in the compound and masterfully maneuvered their way to bin Laden.
In -- heart pounding -- fight these few brave souls brought to justice the terror mastermind.
But Navy SEALs were involved in another famous mission.
In January 2009.
Navy seal team six rescued captain Richard Phillips.
After his cargo ship the -- Alabama had been hijacked by Somali congress.
Thanks got to thank you very much.
-- -- -- The seals shot and killed three Somali pirates and rescued Phillips was being held come pork.
And these are just conditions that made the headlines Navy SEALs continuously put themselves in high risk situations.
Without the recognition.
Being the silent heroes the nameless faceless sailors.
Who stand up fearlessly against the world's most dangerous threats.
Adam brown was already a navy seal when he was blinded in one -- during a training exercise.
And then lost parts of his fingers on his dominant right hand when his humvee flipped and crashed.
Instead of leaving the military with an -- medical discharge -- -- -- with his left hand and then pass the test to become a member.
Of navy seal team six.
Adam was killed in action in Afghanistan march 17 2010.
His remarkable story of bravery is told in the book fearless.
Joining me now the author of the book Eric bland and lieutenant Brian Walesa who -- with Adam on -- It.
-- captured in the sport -- -- tell me what was it about Adam's story that captivated you and said this ought to be a book.
Well governor -- neither of these guys -- -- like all the -- their gift torn nation and madam story really is a gifted it's inspiring.
Because it takes you from a boy who rose up from his darkest places -- to become a seal.
Let's fast forward let's let's go to humvee accident he mention he.
Was driving from a Kabul towards Kandahar and all the sudden I could shoot crash -- slipping in the areas holding on to the right window.
And lands after the cloud of dust clears his buddies come up and they see Adam giving aid to a guy in the back -- Had his -- and Ford driven through shoulder blade.
Blood everywhere chaotic situation and he said there helping amount and then someone finally realizes after about fifteen minutes hey Adam.
Your fingers and you know your hands messed up bunny.
They were gone I mean they were hanging there by tendons and skin and he was giving aid to this guy he's our guy I'm okay I got it gluten is that we -- we -- get this -- -- -- this machine.
And that's just shows a little snippet and a lot of rhetoric through the compassionate hero -- the humble hero.
And he then after they wrapped up -- tenth place its fingers back and had the bandages.
He picked up his rifle and then held security.
For his own -- after he's been after he's been very very severely injured so this of the type of guy he always put others before himself.
And his story -- that was only back to when he was little kid.
And -- all over the top tier to the tip of the spear seal -- six -- his story again it's it's a gift.
It is a remarkable story Brian -- trained with and served with Adam tell me as a fellow -- what kind of person was -- Adam round and -- always puts a smile on everybody's face she mentioned his name -- Going through training first time I met Adam.
I can remember.
It was a long before my time run for some ever seen him and I saw this guy determined -- and just.
Focused on this four -- time run after -- probably just swam and ran in -- school courses.
And I'm -- come across the finish line announced thrown out -- because you pushed himself so hard and right after that it kind of shook it off and kept going.
I mean kind of I think that in itself.
Was just a great example of no matter how hard he would either get hit or hit himself he'd always come back harder mean Erica mentioned the fact.
And now you -- to governor about the fact of losing his you know it's not just his left hand but he also had to teach himself.
A right hand -- -- eye right eye dominant person you shoot left eye dominant and to do it extremely well is unprecedented what I'd never heard that.
Mean I thought typically if you lost and I particularly that your domino line that -- shooting nine anybody as a shooter understands.
That's not minor it's not just like saying all just look out of the -- what.
I mean it's it's your whole hand eye coordination.
The fact that he could even do it to proficiency of going deer hunting would be pretty significant to be able to still qualify for the seals.
I've never heard anything like that Bryant and it is it it is pretty unusual isn't it.
A president who we see Joseph collapsing machines he would look over to me on along on a run a versatile team for and he be handling that you really -- -- -- hammer.
He had an and he used to celebrate that that that agree that aggressive spirit or the warrior -- actually emulated.
And he resonated from -- -- a kind motivated everyone around him you see now with all the people that are backing the -- the movement and backing family in Kelly.
This these -- a lot of people and his his energy and his warrior spirit is kind of taken it's it's taken amazing -- Amazing routes -- tell me what what was the biggest surprise you found out as you did the research for the book fearless and and when you wrote about Adam brown what was it you just step back and said oh my gosh.
Hit his ability to overcome.
And it and -- I mean he came from the darkest hole you can imagine he was addicted to crack.
Him how many people even can make the navy.
Vs the top -- the tip of the -- seal team most people can't get a job working as a clerk -- a store once had gotten on crack on and I'm not not menu honestly when another car his -- said you know I wouldn't volunteer that you -- more drug I wouldn't volunteer -- you -- eleven felons that you felonies that you and been in jail.
He said.
But he said if I'm on the right path I'm gonna tell the truth he and if -- meant to be what I wanna be and -- just to get in the navy he didn't know where he was gonna get and a really that is what it inspired me the most to see how this this kid we all know him we all know Adam brown the kid that was a nice kid to open people's doors that was honest.
Would not -- to his parents.
That one time.
The horrible drug one time you and it turned to the world upside down and then to come back.
And use that energy that daredevil streak in him to channel it into something for good.
But the ultimate levels and never quit never give up.
That's why brown is in fact a miraculous life.
And his legacy will be preserved forever in the wonderful book you've done called fearless.
Perry plans book fearless is available at bookstores everywhere but all of our audience will be getting a copy.
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I stayed at this hotel while driving from Kansas to South Texas and the Lady at the front desk was very pleasant. My Room was Clean,,, Quiet,,, and the TV Programming was very good. Breakfast in the Morning was expanded and Fresh. I reccomend this Hotel.
- Reservation Options:
- TripAdvisor is proud to partner with Cheap Tickets, Orbitz, Travelocity, Tingo and HotelsClick so you can book your Motel 6 Temple reservations with confidence. We help millions of travellers each month to find the perfect hotel for both holiday and business trips, always with the best discounts and special offers.


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Monday, May 21, 2012
The Play Date Cafe 132
17 comments:
- Lynn said...
Those Memory Box dies are incredible...such absolutely fabulous detail. Love your take on the challenge.
- May 21, 2012
- Kara Lynne said...
Too funny, I just posted a bird on a branch for the same challenge not 1/2 hour earlier. We must be on the same wave length. I love the dies you used.
- May 21, 2012
- Stacy said...
Excellent take on this weeks color challenge. Love how the bird just pops off the branch. Thanks for playing with us at The Play Date Cafe!
- May 21, 2012
- Michelle said...
Wonderful card! Love those dies!
- May 21, 2012
- Donna Bowman said...
Great CAS card! Love the dimensional tree and bird! Thanks for joining us this week at The Play Date Cafe.
- May 21, 2012
- Jinny Newlin said...
This is gorgeous, Lisa! LOVE the way everything pops on your bright white background, and the font of your sentiment is fantastic!
- May 21, 2012
- sanketi said...
Lovely! am so glad I placed my order for that bird ...thanks for the inspiration!
- May 21, 2012
- Tenia Nelson said...
Very very pretty!!!
- May 21, 2012
- Meadie said...
Your card is just gorgeous, hugs x x
- May 22, 2012
- Brenda said...
This card just jumps off the page. Great idea. Really stands out.
- May 22, 2012
- Jenny said...
I think I see a winner here! What an amazing card... You continue to wow me with those dies! Love the leaf as a wing :0)
Jenny x - May 22, 2012
- Larissa Heskett said...
This is BEAUTIFUL!! I LOVE the FUN Design and cute details!! THANKS for sharing and have a FABULOUS WEEK!! =)
- May 23, 2012
- Sleepy Stamper said...
Love this card! That little bird is just so perfect with the sentiment and the CAS look to this card! Beautiful work!
Anitraj - May 23, 2012
- Barb said...
wow! what a beautiful card!!! love your designs :)
- May 23, 2012
- Virginia L. said...
I adore this so much, Lisa! Congrats on your Fab 4 win over PDCC!! Way to go!!!
- May 24, 2012
- Kelly Griglione said...
ooooh, love the simplicity ... beautiful card! Congrats on your win!
- May 24, 2012
- Quip1320 said...
This is simply stunning! Congrats!
- May 25, 2012


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Zayden Plaid Vest
Take on fall's structured trend in our latest vest. In need-now plaid with striking button-detail and a shield plaque, this layering piece adds the extra dose of style you've been looking for.
• Plaid vest
• Patterned exterior with contrasting front placket. Button detail at right side of placket. Logo-embossed shield plaque at left chest. Welt pockets at lower front. Tab with buckle at lower back.
• Front zipper closure
• 100% Cotton
• Hand wash
- Need Help?
- Customer Service


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Credits & Info
- Uploaded
- Feb 6, 2010 | 4:44 AM EST
- Daily Feature February 7, 2010
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Author Comments
A point-and-click thriller, second chapter of the Foreign Creature series.
On a planet far away an evil King has made a clone of himself to create an even more powerful ruler. This clone has been sent to planet Earth to grow stronger and breed an army to take back home.
Play the role of a blood-thirsty alien sent to planet Earth to grow stronger and breed an evil army to take back home.
Point-and-click to interact with the environment.
There are 2 bonus scenes after win the game.
There are 2 different endings in the bonus scene #2.
Added medals, thanks Tom.
Rated 3 / 5 stars August 22, 2013
The game never showed us what the creature was up to.
All you did is making us guess all the time with alot of amount of clicking.
After all this game is nothing but a waste of time.
If there is a third game then it should tell us how the creature wants to kill or escape in each scene.
Rated 3.5 / 5 stars August 21, 2013
well It has been such a great game until now but it would have been great with it being available with hints or having more sequels :P instead of immediately giving it the part where it infected the whole world but nothing more, this is a good game therefore I give it 3.5 stars.. good game overall Awakens and Belugerin...
Rated 5 / 5 stars August 18, 2013
I'm giving you five because I'm REALLY enjoying this, but the cop on the left is also bugged for me...too bad, I was looking forward to seeing this through!
Rated 3.5 / 5 stars August 7, 2013
The bottom left cop is bugged. Can't get pasted it. So can't give a full review right now.


text:
dude, you should add all of these to the original list, get 'em compiled.
You board will never ask you if it's rocker looks fat.
You can make your board 'looser' or 'tighter' to taste by altering fins.
You can ride your board for hours without it getting sore.
You don't have to get stroppy about other blokes openly admiring your board
you'll never have a fight over a surf board.
a surfboard is yours for as long as you want it
your board will never get drunk and throw up on your carpet
Your board will understand if you don't take it out at the weekend
Your board won't get a rash when you wax it


text:
We plan to come back, thanks to attentive stuff, the hotel is beautiful and cosy. Excellent location. The art collection of the hotel is very interesting, and its a real pleasure to have tea in the lobby area...
- Also Known As:
- Cambon Hotel Paris
- Official Description (provided by the hotel):
- A hotel to discover or to be discovered in next to no time whose regular guests hand the address by word of mouth.An exceptional site: near the Concorde Place and the Tuileries gardens, in one of the most prestigious street of Paris: Cambon Street, the historical headquarter of Coco Chanel, but also in the heart of the city's most elegant shopping and art district, and just next door, place Vendôme for its well-known jewelleries,Few steps from the Louvre and Orsay Museums, Opera Garnier and 10 minutes walking distance from the Galeries Lafayette flagship store. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Booking.com, Orbitz, Priceline, Hotels.com, Agoda, Travelocity and Asiatravel.com Holdings so you can book your Hotel Cambon reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.


text:
"What's Happening at West Virginia Archives and History"
Important Notice: Beginning Monday, November 4, 2013, an upgrade is being made to the elevator that provides access to Archives and History closed collections. This upgrade may take up to ten weeks to complete. The library is implementing the following procedures for retrieving materials for patron use during this period:
1. Library patrons who plan to use specialized collections, such as manuscripts and land books, that are housed on other floors must submit requests for these materials at least two days in advance of the day they intend to visit the library. On Saturdays, staff may not be able to provide materials that have not been requested in advance. 2. Staff will pull materials from other floors once each hour.
2. Staff will pull materials from other floors once each hour.
Whether studying for the Golden Horseshoe exam, researching your family lineage, or looking for information on the history of West Virginia, the Archives and History website is the premier on-line resource. The site features primary and secondary source documents, photographs, and databases; lists collections and other materials available in the State Archives; and provides research guides and assistance for genealogists.
The academic challenge for eighth gradersto test their knowledge of West Virginia
Nathan Bay Scott, who served as United States senator from West Virginia from 1899 to 1911, was born on December 18, 1842
Click here if you would like to join our mailing list.
Articles and documents on this site contain the exact wording of the original. Comments and suggestions about the website www.wvculture.org/history may be addressed to Mary Johnson at the West Virginia State Archives. The Archives will not answer e-mail research requests. All research requests must be submitted in writing to the Archives and History Library; The Culture Center; 1900 Kanawha Boulevard, E.; Charleston, WV 25305-0300. Requests from outside West Virginia must be accompanied by a $15 research fee; a $5 research fee must accompany in-state requests (checks made payable to Division of Culture and History). Please read the Archives' listing of services available for more details on research correspondence. Important information regarding checks received for payments


text:
Eamon Horan reports on the French and English clubs' decision to withdraw from the Heineken Cup
Ulster have injury worries as the provinces gear up for Heineken Cup action.
Dave Kearney sees the next two weeks as being pivotal in Leinster's season.
The former Ireland and Ulster fullback talk about Ulster's preparations for the weekend.
Former Ireland international Frankie Sheehan discusses his retirement from rugby
Hugh Cahill reports on Ulster's win over Zebre
Zane Kirchner gives his reaction to Leinster's win over Scarlets
Gerry Thornley gives his assessment of the French clubs' decision to stick with the Heineken Cup.
Justin Treacy looks ahead to the weekend's action and hears from Leinster's Guy Easterby


text:
We stayed for a night at this hotel. The room was a good acceptable size with bright laminated flooring. It was clean enough and had free wifi but no coffee-making facilities. The bathroom was okay. The breakfast area was unappealing and so was the food. For one night passing through, it's bearable.
- Also Known As:
- Tatlises Hotel Kusadasi
- Official Description (provided by the hotel):
- Kusadasi, one of Turkey's principle holiday resorts, offers an excellent environment for an unforgettable holiday. Situated on the west coast of Turkey- 90km south of Izmir, Kusadasi, is reputed for one of the most attractive city of the Aegean, as it is close to the important historical sites including Ephesus, Didyma, Priene, Miletos-the principals of ancient times, and ideal for sightseers.Yacht Marina 1 km, Palm Beach 1 km, City Center 700 mt, Harbour 2 km, Adnan Menderes Airport (ADB) 70 km, Ephesus 20 km, ... more less
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- TripAdvisor is proud to partner with Booking.com, Priceline, Travelocity, Otel and Hotels.com so you can book your Tatlises Hotel reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.


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Phenol extraction
Posted 15 July 2010 - 05:34 AM
I'm new to phenol/Chlorophorm DNA extraction, and I tried today with my Boss and we get an interesting, but not wanted, result...
A milk-like solution, without aquaeous/phenolic difference after centrifugation...
What I did :
Ressuspend cells in 4ml of (100mM Tris pH=8, 50mM EDTA pH=8, 50mM NaCl)
Add SDS to final concentration 1%
Add Sarcosyl to final concentration 0,8%
Mix gently by inverting
Adding 4ml of pH8 saturated phenol
Mix several time by inverting
And I get this milk-like solution...
Phenol was yellow.
Any idea ?
Thanks for your help !
Posted 15 July 2010 - 05:37 AM
Posted 15 July 2010 - 05:39 AM
Posted 15 July 2010 - 05:46 AM
and the speed has to be ok.
Maybe there is something wrong with the mixture at the start, you are sure the chloroform mixture is ok? (phenol/chloroform/isoamyl alcohol 24/1 ratio?)
Posted 15 July 2010 - 05:49 AM
We were planning to do phenole extraction twince, then phenol chlorophorm twice and to finish, chlorophorm only twice before washind with ethanol.
I note for next time to extand centrifugation time. How much do you suggest ?
Posted 15 July 2010 - 06:29 AM
Posted 15 July 2010 - 06:48 AM
I found in an article they are using TE buffer with 100mM NaCl added. I think I will try with this solution.
If anyone have a good idea, I will be pleased to hear it !
Have au good day
Posted 15 July 2010 - 08:51 AM
Do you have a newer bottle?
A. Einstein
Posted 15 July 2010 - 05:25 PM
Isn't yellow phenol, oxidized phenol?
Do you have a newer bottle?
yup, yellow and as it gets even more oxidized the phenol turns red.
However if 0.1% w/v 8-hydroxyquinoline, a reducing agent used to protect phenol from oxidative damage was added, the phenol solution will turn bight yellow.
From Sluvah protocol I believe only a phenol solution is being used. No chloroform was added, so you can't obtained the aqueous-organic layer.
the composition of phenol/chloroform solution is
25:24:1 = phenol solution:chloroform:isoamyl alcohol
The phenol solution is composed of
10mM TE, 1mM EDTA solution saturated with phenol.
Add 0.1% w/v 8-hydroxyquinoline to your phenol solution, else it will get oxidised very quickly.
For DNA extraction the pH of the phenol solution is pH 8
For RNA extraction the pH of the phenol solution is pH 4.5
Posted 16 July 2010 - 04:25 AM
From Sluvah protocol I believe only a phenol solution is being used. No chloroform was added, so you can't obtained the aqueous-organic layer.
Thats where I was stuck, I couldnt understand it how he wanted to obtain that layer when using only phenol.
Posted 18 July 2010 - 11:57 PM
Sorry to answer so late,
Our phenol is yellow-ish becaus we add inside 8-hydroxiquinoline to prevent oxydation. It's fairly new, we bought it 1month ago and open it last week.
I will try phenol/chlorophorm only extraction also. Should-I do only twice ? Or try it more time to compense phenol extraction absence ?
Thanks for your help !
Posted 19 July 2010 - 07:40 PM
I will try phenol/chlorophorm only extraction also. Should-I do only twice ? Or try it more time to compense phenol extraction absence ?
Thanks for your help !
Sorry, I don't quite understand what you mean. Could you rephrase what you were saying?
Posted 20 July 2010 - 12:52 AM
My boss' protocol for phenol then Phenol/Chloroform then Chloroform extraction have 6 steps because each extraction (phenol, Phenol/Chloroform , Chloroform) are made twice.
For the Phenol/Chloroform and Chloroform extraction, should I do twice Phenol/Chloroform extraction then twice Chloroform extraction, or should I do it more times ? (Three time for example).
There should be a layer even with phenol only extraction, but it seems to be hardest to get.
Posted 20 July 2010 - 01:53 AM
maybe its better to post the protocol here.
Posted 20 July 2010 - 03:52 AM
(For archaea, but after the cell lysis, there shouldn't be any differences, no ?)
500ml archaea culture is pelleted and washed on N100 buffer (100mM Tris pH8, 100mM NaCl, 50mM EDTA pH8) then resuspended in 2 to 5 ml of N100 buffer.
Then, it's frozen at -80°C for several minutes then unfrozen at room temperature. The SDS is added (1% final) then the sarkosyl (0,8% final) (These two are probably too concentrated.)
After, 1 vol of phenol is added then mixed by gently inverting for 15 minutes.
Then, centrifugation 5 min at 4000RPM to separate layers. The aqueous layer is preleved, and a new phenol extraction is did (same thing, add a volume of phenol, mix, centrifugate).
Then, same thing but with Phenol/Chloroform (Two extraction)
Then, same thing but with Chloroform (Two extraction)
Then, precipitation with 100% ethanol, then with 70% ethanol.
After dessication, ressuspension of the DNA into the TE
Test of phenol absence by restriction enzyme digestion.
I think his protocol may work whis HIS strains, (streptomyces) but not with mine...
I plan to use an other cell suspension buffer (TE + NaCl 100mM) and try with and without the phenol-only extraction step..
Thanks for your kind help !


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We're willing to bet even the most die-hard Mopar fan hasn't seen a 1963 Dodge Custom 880 hardtop before. Only 1275 were built that... More
If you like your cars with pedigrees few can match the details that accompany this potent 1971 Super Bee. Build sheets MSO Owner Card... More
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text:
I'm working with a local business that has a storefront and an online catalog selling wine, and they're wondering how they can best improve their search engine results.
If you search for their actual business name, they're the top link returned, but if you merely put in something generic like 'wine' and the city name, they're sitting way back on the fourth page of results. They're not trying to compete with large wholesalers, but they would like greater exposure for when people search for local wine retailers.
What can they can do to improve their local search results? Would the most impact come from having their name and URL published on other sites that specifically focus on their geographic area? (Such as sites that do local business reviews or focus on local events such as wine tastings?)
I'm not an expert in SEO, so I would appreciate any feedback or ideas that I could pass on to them. Thanks!


text:
Dom Mazzetti v. White Rappers
The hand shoes destroyed me lmao. "CLEAR!"
Dom Mazzetti v. White Rappers
The hand shoes destroyed me lmao. "CLEAR!"
Nas he didn't.
Valandui's Weekly Music Video
What are the odds that Dom Mazzetti is Dominizzo?
2013 Goals:
1) Win the next game.
2) See goal #1
"The problem with internet quotes is verifying their authenticity."
-Abraham Lincoln
The NEW Front Office Plan
for our Miami Dolphins:
-Hire the FH Staff, ad hoc.
... Could we really do worse?!?
(I'll accept the newly created position of beverage consultant)
Osah lama Lakers!
"Martining" things is no way to go through life.
Don't be a Martin.
"I have one dance move. It's called the retard face with the rotating arm."
I sold weed fa like two weeks and then I got grounded.
But when the trumpets blew again and the knights charged, the name they cried was, "Stannis! Stannis! Stannis!"
"... and blastin ye-yo off of *******..."
"... classes are like abortions, you have the option."
Dom rules.


text:
Singletons everywhere, rejoice: We've got the best reason ever to fly solo this Valentine's Day. Netflix just released a gorgeous, foreboding trailer for House of Cards, which announces the second season will premiere on February 14, 2014. If you haven't seen the political thriller yet — cue an audible gasp, read
If you're looking for a great holiday gift for your girlfriends (or just want to splurge on yourself), check out Urban Chic's Perfect Gift Holiday Trunk Show this weekend. There's a kick-off fete hosted by White + Warren, Loren Hope, and Blair Ritchey on Thursday night, and the store will be offering 15% off from read


text:
Videos for #101708 RTR Pulse 4.6 w/ 2.4GHz
HPI PULSE 4.6 Buggy RTR
HPI PULSE 4.6 Buggy RTR
With an all-out racing design, amazing features and massive 4.6 engine, the Pulse is a seriously impressive buggy! Whether you take it to a huge field for playing or your local race track for serious racing, you will love the power, response and strength of this awesome car!
- Pulse 4.6 Buggy in action!