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If you are looking to lose weight, you are finest off looking at the successes in the weight reduction field, those that are standing the test of time. The Diet Solution Program appears to be one of those being the top selling eBook in the field because its introduction in 2010.
The writer of the publication is Isobel De Rios, a really well qualified expert in nutrition by having numerous degrees and diplomas. The program was established as an antidote to all the get clear of weight swift, put it back on just as quick items on the market and looking at the problem from a dietary viewpoint.
Unlike several nutritional programs on the market this is not some faddish diet like consuming nine bananas a day or just drinking grapefruit juice. This is a diet that introduces exactly what to countless is a novel concept, ingesting well and making way of living changes that fit your metabolic kind.
Being based on your metabolic kind is a reasonably new idea and not one without criticism but it does appear to make sense on an instinctive degree. Essentially we are all different and our nutritional demands differ according to our metabolic rate. This is not some sort of short how to book, it is in fact almost 100 web pages long and begins of with an comprehensive questionnaire to establish your metabolic kind so your personal diet are able to be tailor made in consequence. That diet will certainly be based on the food packs that are best for your metabolic kind, offering you the kinds and amounts of carbs, fats and protein you must be eating on a everyday basis.
The distinction with The Diet Solution Program is that you have the option to figure out just what may be the finest diet for you and you then have a choice of around sixty days of meal deals to work at, all based around that body type. This is definitely a healthy and balanced eating plan with whole lots of fruit and veg and a mix of the vital fats carbs and proteins ideal for your body type according to those metabolic concepts.
You get a expansive dinner deal based on your individual body kind which provides you sixty days of meals with some bites to eat and lots of recipes. This is a diet based on healthy and balanced eating with no narrow focus issues like cutting out carbs, however ingesting bunches of fresh fruit and vegetables by having natural herbs and spices. A expansive list of foods for each body sort is consisted of. It isn't really rocket science but it does appear to work.
There are lots of reviews about this program featuring one from the author's mother that was a diabetic and having actually utilized the diet herself is now far better. Numerous diabetic problems are created by diet and it is an increasing issue in the west so anything that assists with those stats has certainly to be welcomed.
This is a full bundle nonetheless and if you were thinking you can get away without any activity, well you are able to but it does aid if you boost your heart fee when in a while. So there are some extremely full workout handbooks by having instructions and helpful photographs giving you all the cardio and resistance workouts you might need to speed up the weight loss procedure. Provided the number of complimentary diet solution program review , this does seem to be to work.


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Enjoy an indulgent spa break with QHotels
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QHotels spa portfolio will meet your every need and surpass all your expectations.
Your indulgent spa break includes:
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This offer is subject to availability at QHotels with spa facilities.All treatments booked are subject to the spas cancellation policy, which you should enquire about upon booking your treatment with your preferred spa. You may be asked to split your spa treatments over two days. Please note spa treatment times are subject to change. Leisure and spa facilities vary at each property, please check at time of booking. Guests staying on a one night spa break will receive 1 x 60 minute spa ritual or 2 x 30 minute mini spa treatments. Guests staying on a two night spa break will receive 1 x 60 minute spa ritual and 1 x 30 minute mini spa treatment or 3 x 30 minute mini spa treatments. Please note your 60 minute spa ritual includes 50 minute treatment time and 10 minutes client care time. Please note your 30 minute spa treatment includes 25 minutes treatment time and 5 minutes client care time. Prices are based on two people sharing a standard double or twin room on a dinner, bed & breakfast basis, subject to availability. Guests that stay on a Sunday night will be asked to take their spa treatments after 2pm on the Sunday or request that you have them on a Monday morning. Guests staying on a Friday night package will need to take their spa treatments on the Friday or before 11am on the Saturday morning. Supplements apply for any room upgrades. A £50 per person per night single supplement applies. However, please note that certain spa hotels do have triple bedrooms. Please enquire upon booking your spa break. Please note that a £26 per person allowance is made for a three-course evening meal. Slippers and robes are available for the duration of your stay only. Cannot be used in conjunction with any other offer. Supplements apply for any room upgrades. You will be asked to pay a non-refundable, non-transferable deposit of 25% on booking, the rest is payable on departure or in the event of cancellation.
From £178.00
The Westerwood Hotel & Golf Resort is a fantastic four star hotel located just a fifteen minute drive from both Glasgow and Stirling.. The hotel enjoys a tranquil and picturesque setting and with excellent facilities including an 18 hole golf course, leisure club, spa and fantastic meeting rooms, this spectacular hotel near Glasgow is perfect for business travellers or those looking for a leisure break, spa break or golfing break.
Aldwark Manor Golf & Spa Hotel, York, is set in the stunning North Yorkshire Moors, just a nip from the A59 ideally positioned between historic Harrogate and York, providing a great escape from the hustle and bustle of daily life. Picturesque villages and rolling moorland allow you to relax, unwind and enjoy all the delights that this exceptional North Yorkshire hotel has to offer.
For a truly magnificent hotel in Cheshire, there is nothing grander than the four star Crewe Hall. Standing majestically in beautiful grounds the hotel is a stunning mix of contemporary chic and elegant tradition. This Cheshire hotel has all the facilities for those travelling on business or pleasure, as well as being a fabulous wedding venue.
As far as hotels in Cheshire go, The Park Royal couldn't be better located. This fantastic four star hotel is just off the M56 and a short drive to Chester and Liverpool and only 14 miles from Manchester Airport. The Park Royal offers fantastic facilities making it perfect for a leisure break or business trip.
Tankersley Manor near Sheffield is a fantastic business hotel but also a luxury hotel for leisure guests. Just off J36 of the M1, the stunning hotel is built around a 17th century residence, it has all the modern facilities of a four star hotel coupled with charming original features.
Forest Pines Hotel & Golf Resort, North Lincolnshire, is ideally located just off the M180. Featuring a 27 hole golf course, luxurious spa, fine dining and superb leisure facilities, Forest Pines is ideal for any occasion from weddings and conferences to spa and golf breaks.
When it comes to hotels in Nottingham, the four star Nottingham Belfry is one of the most contemporary and stylishly designed. The hotel is just 5 miles from the bustling city centre, just a mile off the M1 and 15 miles from East Midlands Airport. With 13 conference and meeting rooms, this is the largest conference venue in Nottingham. The Nottingham Belfry is the perfect hotel for business travellers and leisure guests.
Set in a UNESCO World Heritage site, the Telford Hotel & Golf Resort is a gem in the Shropshire countryside. The four star hotel offers total luxury and the facilities include an 18 hole golf course, Spa and Leisure Club, 114 bedrooms and 8 conference and meeting rooms for up to 350 people.
Chesford Grange is a fantastic four-star Warwickshire hotel offering everything you could possible wish for in a hotel. Historic charm is coupled with contemporary glamour, all set in beautiful grounds on the edge of the River Avon. Chesford Grange boasts luxurious bedrooms, an excellent restaurant and some of the best conference and event space in Warwickshire.
The four star Stratford Manor hotel is situtated just off the M40 in 21 acres of stunning Warwickshire countryside. The hotel is just 5 minutes from the centre of Stratford-upon-Avon and all the sights and history that can be found there. The hotel offers fantastic facilities whether travelling on business or for pleasure.
Hellidon Lakes Golf & Spa Hotel offers fantastic four star accommodation set in 220 acres of beautiful countryside. The hotel is close to major motorway networks, Silverstone racing circuit and on the doorstep to the picturesque Northamptonshire countryside. The hotel offers fantastic facilities including a 27 hole golf course and 24 state-of-the-art meeting rooms, an ideal hotel for both business and pleasure.
The Cambridge Belfry enjoys a fantastic location close to the A1 and M11 and just 10 miles to the stunning university city of Cambridge. The hotel offers excellent facilities including 120 bedrooms, a leisure club and 8 conference and meeting rooms. If you are looking for a place to stay, whether it is for business or pleasure, look no further than this fabulous four star Cambridge hotel.
The Oxford Belfry is a fantastic four star hotel near Oxford. Just off the M40, the hotel is on the outskirts of the pretty town of Thame and is a great base for exploring the Oxfordshire countryside. The Oxford Belfry has great facilities including 154 bedrooms and an excellent leisure club. With 17 conference and meeting rooms the hotel is an excellent conference venue near Oxford.
The Cheltenham Chase Hotel is a perfectly placed four star hotel for visiting the sights of Cheltenham and Gloucester and the surrounding beauty of the Cotswolds. If you need a hotel near Cheltenham Race Course, look no further. The hotel offers fantastic facilities including sumptuous bedrooms, conference and meeting rooms and a superb leisure club and spa.
No other hotel in Hampshire will come close to the four star luxury of The Hampshire Court Hotel. This hotel in Basingstoke is only 3 miles from the M3 and 45 minutes from London, making it an ideal business hotel or a perfect place for a leisure break. The hotel offers fantastic conference and meeting facilities, in fact it is the largest conference hotel in Hampshire.
Norton Park, Winchester resides in 54 acres of peaceful grounds and is only minutes from the A34 with easy access to the M3, M4 and M27 motorways. From entering our bright reception area to retiring in one of our 175 immaculate bedrooms, you're constantly reassured that your stay at this spectacular Hampshire hotel will be one to remember.
The Ashford International Hotel is superbly situated just 5 minutes from the Eurostar, 20 minutes from Le Shuttle, 20 minutes drive from Dover Ferry Port, 26 minutes via high-speed train to Westfield Stratford City and 37 minutes via Hi-Speed link to London St. Pancras. The hotel is an ideally located hotel in Kent for both business and pleasure, offering fantastic four star facilities including a magnificent leisure club and spa and state-of-the-art conference and meeting rooms.
In the heart of Kent, Bridgewood Manor is a highly accessible hotel, just 50 minutes from central London and only 42 minutes by high speed train to St Pancras International, with excellent links to the M2, M20 & M25 motorways.
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subset: commoncrawl


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Few things in our society cause as much arousal as sex. Sex is one of the most fundamental human activities, but God forbid it if we do it too much, too little, with a black person, with a member of the same sex, by ourselves or with more than one other person. However, as you can see with the above statement, our views on sex are in a constant state of change: it's no longer a big deal to have, for example, interracial sex or gay sex, and Sex and the City made it a-okay for women to masturbate, which is remarkable considering how even forty years ago, the female orgasm was seen as a lie.
Tides have turned since then, and any man that claims that the female orgasm does not exist does it at the peril of his own mockery. In fact, nowadays, it's seen as a medical problem if women cannot achieve orgasm and some are concerned that the medical-pharmaceutical industry is taking advantage of this to their own ends. Is the medical community following the suit of the music, publishing, gaming, broadcasting, movie, and advertising industry to capitalize upon sex? (On a side note, here is an interesting Cracked article on how porn has shaped the modern world:Â http://www.cracked.com/article_18888_5-ways-porn-created-modern-world.html).
The New View Campaign is a New York based grassroots campaign that exposes the ways by which pharmaceutical companies oversimplify and exploit messages about sexuality in order to sell drugs. While certainly not all medical research is biased, they believe that biased research indeed exists and is more widespread than currently believed. According to them, "sexual problems are complicated, sexuality is diverse, and no drug is without side effects." Sexuality is a very complex matter that is related to many aspects of life, and some in the medical community are trying to simplify it into a problem that a few pills can fix. What is your take on this issue? Feel Free to comment in the section below!
(Photo from: depositphotos.com)


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You can get a high quality look here as well.
16 thoughts on "The Cataclysm Cinematic drops before Blizzcon!"
Epic is the only word good enough to describe this.
agreed i wonder if anyone will complain about the TV commercial because pearl harbor happened on December 7th and that was a cataclysmic event. thoughts?
Amazing intro. So now, we have the release date, and NOW we have the trailer…what does that leave them to reveal at Blizzcon this year? New IP, perhaps Diablo III is farther along than we thought….
meh…nice scenic buildings, would have liked to see the worgen and the goblins. The "Trap Jaw" Dragon is kinda a dumb idea.
Really nice, but i hope blizzard starts working on Warcraft 4, when they are done whit everything else…
oooooohhhhh boy… awesome stuff, as always!!
Eh, I wasn't too impressed with this. On one hand, blizzard does have their usual touch when it comes to designing cinematics – Detail and life everywhere. No doubt that this is apparent in the video. Blizzard also went above and beyond improving their CGI videos once again. However, I am saddened to say that I got what I expected. I had predicted the video would have been "A dragon flying around" and guess what? Blizzard could have done so much more with this and made this so much more impressive, I think they just took the easy route.
This is just epic,amazing,fantastic and… late ?
Why it is epic ? First of all the design, they clearly did a good job making the zones look like what they do in game. Secondly the music, really the music is the biggest reason this trailer is so awesome, it honestly felt i didnt watch the game trailer but a trailer of the upcoming Warcraft movie.
And why did i say it was late ? Well, by this time we could all imagine that the trailer was going to be about the fierce dragon that spits fire like it have swallowed a can of chili's, i think most of us would want to see something about the races instead of the zone tearing. but every expansion movie blizzard have made for WoW has been about that last boss fight you do untill the next trailer for the next expansion hits the net.
Anyway i would of course love to hear Scotts,Randy and Henry's thoughts on this, and maybe talk about some of the things i said
All in all its a epic trailer with a taste of chili pepper!
I have to say if Blizzard ever gets tired of the video game line (and really it's just a passing fad. lol) they could make a killing as an animation house.
The intro was epic though it doesn't compare with WotLK intro. That one had poetry and a great juxtaposition between a father's loving words to his son and a villain raising an undead monster. This is more "I broke it. Yeah. What are you going to do about it!"
That said, I can' be made about a dragon raining destruction on all we know that is familiar in Azeroth. I especially like how the dragon ends up where the dragon head usually hangs in SW. Consider the black dragonflight avenged.
i agree with batallion… now what are they gonna give us at blizzcon?… something even more awesome?
I did call in on this one. Again, it was like the roller coaster effect for me. I didn't quite make it to the top. Even when it was over, it still left me with anticipation rather than the exciting rush you get when you plunge down that first drop. I was left hanging on the incline of the tracks.
High expectations… or course. That's always the case. You have to learn to beat it. And it was predictable in knowing about the fearless dragon flying around causing mayhem, which is okay cause that's expected.
Blizzard spent too much time awakening the dragon. Not enough destruction and in that, not enough destruction where you get to see the races and other wild life (reactions maybe). That was the big miss right there. Everyone wants to see the races, because the last one we didn't, and we're ready for it again. They could have gone without the overly drawn out and redundant voice over. Too much "pain, rage, burn" etc…. all means the same thing.
My only advice… games and movies are different. If you're creating a trailer relative to what you would see for a movie, then you (Blizzard) need a to hire a film director with the knowledge for story flow and content, and a film editor.
At any rate, it was a tie with the last trailer. The first two trailers are still the best.
I was starting to doubt they'd give us a proper CGI trailer we deserve. I was wrong! This is the most epic thing I have ever seen! I had to watch it twice because I couldn't take it all in the first time! Don't understand why it wasn't released on blizzcon, or maybe kept a secret for when peeps start up the game when it's released, but I'm not complaining!
Hey wart, they need the trapdoor mouth because it marks the entrance to an instance: The Deathwing raid; he's so massive you gotta wait until he's sleeping, sneak into the instance in his mouth and kill him from the inside out. As you navigate his body, you fight each organ as an individual boss. But really.. they need to change the mechanics of fighting dragons instead of enlarging the circular attack range to kill them. Hope they do something cool and new with dragon fights in cata.
Okay, Scott/Randy… that's two in two weeks… please, please, please, PLEASE predict that there's no way in hell that Blizzard will drop the price of Cataclysm to $5.
Well naah…
Still didn't get me fired enough as the TBC one did…
For me personally its TBC -> WoTLK -> This one here…


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Amber-colored beverage from planet Ennan VI.
Katherine Pulaski brought a bottle of this ale to a gathering in Riker's quarters for a homemade breakfast. She believed Riker's omelets deserved no less, but she later ate her words (so to speak).
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Following is a list of faculty at UPenn and Drexel involved in the IGERT program. For more information on a particular faculty member, follow the link from their name. If you are a faculty member and would like to become involved in the Drexel-Penn IGERT, contact Danielle Tadros.
University of Pennsylvania
Bonnell, Materials Science & Engineering
H. Bau, Mechanical Eng & Mechanics Alan
MacDiarmid, Chemistry |
Drexel University Michel Barsoum, Materials Science & Engineering
DiNardo, Physics Bakhtier Farouk, Mechanical Eng and Mechanics
(Gary) Friedman, Electrical & Computer Eng Yury Gogotsi, Materials Science & Engineering Surya Kalidindi, Materials Science & Engineering
Peter Lelkes, Biomedical Engineering
M. Lowman, Chemical Engineering
Hongseok (Moses) Noh, Mechanical Eng & Mechanics
Kambiz Pourrezaei, Biomedical Engineering Roberto Ramos, Physics Caroline Schauer, Materials Science and Engineering Wei-Heng Shih, Materials Science & Engineering Kevin Shuford, Chemistry Karl Sohlberg, Chemistry Jonathan Spanier, Materials Science & Eng Steve Wrenn, Chemical Engineering |


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- Product nameAnti-HDAC1 antibodySee all HDAC1 primary antibodies ...
- DescriptionRabbit polyclonal to HDAC1
- Tested applicationsICC/IF, WB, IP, IHC-P more details
- Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human HDAC1.
(Peptide available as ab20434.)
- Positive control
- This antibody gave a positive signal in Hela whole cell lysate and Human Breast Carcinoma.
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 - Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Our Abpromise guarantee covers the use of ab19845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Notes |
ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. |
WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human HDAC1 peptide (ab20434). |
IP | IP: Use at an assay dependent concentration. |
IHC-P | IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
- FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
- Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
- Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
- Post-translational
modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome. - Cellular localizationNucleus.
Target information above from: UniProt accession Q13547 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) . -
Database links
- Entrez Gene: 404126 Cow
- Entrez Gene: 3065 Human
- Entrez Gene: 433759 Mouse
- Entrez Gene: 297893 Rat
- Omim: 601241 Human
- SwissProt: Q32PJ8 Cow
- SwissProt: Q13547 Human
- SwissProt: O09106 Mouse
- SwissProt: Q4QQW4 Rat
- Unigene: 88556 Human
- Unigene: 202504 Mouse
- Unigene: 391033 Mouse
- Unigene: 1863 Rat
see all -
Alternative names
- DKFZp686H12203 antibody
- GON 10 antibody
- HD 1 antibody
- HD1 antibody
- HDAC 1 antibody
- HDAC1 antibody
- hdac1: histone deacetylase 1 antibody
- HDAC1_HUMAN antibody
- Histone deacetylase 1 (HD1) antibody
- Histone deacetylase 1 antibody
- Reduced potassium dependency yeast homolog like 1 antibody
- RPD3 (reduced potassium dependency yeast homolog) like 1 antibody
- RPD3 (reduced potassium dependency) antibody
- RPD3 antibody
- RPD3L1 antibody
see all
Anti-HDAC1 antibody images
The image shows staining of human tonsil tissue using ab19845. Staining was nuclear and was equally successful using Tris EDTA pH9 or Citrate pH6 for antigen retrieval. Staining was prevalent in almost all cellular compartments of the tonsil.
All lanes : Anti-HDAC1 antibody (ab19845) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate withHuman HDAC1 peptide (ab20434) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab7090) at 1/5000 dilution
Predicted band size : 55 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?) -
ICC/IF image of ab19845 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab19845, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Panel A shows localisation of ab19845 to the nuclei, Panel B has the Alexa Fluor® 488 channel removed for comparison.
ab19845 at a 1/3000 dilution staining asynchronous HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and immunofluorescently labelled with ab19845 for 30 minutes at room temperature. Bound antibody was detected using a Cy3 conjugated goat anti-rabbit antibody. Nuclei were visuallised using DAPI staining. The antibody was found to be highly enriched in the nucleus.
This image is courtesy of an Abreview submitted by Kirk McManus.
ICC/IF image of ab19845 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19845, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 1µg/ml, and in 100% methanol fixed (5 min) MCF7 and HepG2 cells at 1µg/ml
IHC image of HDAC1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19845, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
HDAC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab19845.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 60ka: HDAC1.
References for Anti-HDAC1 antibody (ab19845)
This product has been referenced in:
- Takase K et al. Monoaminergic and neuropeptidergic neurons have distinct expression profiles of histone deacetylases. PLoS One 8:e58473 (2013). ICC/IF ; Mouse . Read more (PubMed: 23469282) »
- Wells CE et al. Inhibition of histone deacetylase 3 causes replication stress in cutaneous T cell lymphoma. PLoS One 8:e68915 (2013). WB ; Mouse . Read more (PubMed: 23894374) »


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Dewars White Label Blended Scotch
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375ml Bottle $318.97
Case of 24
375ml Bottle $318.97
Case of 24
Item #161363
Size: 375ml
Type: Scotch
Varietal: Blended
Country: Scotland
Proof: 86
Prices, promotions and availability may vary by store and online.
Cutty Sark Blended Scotch
"Pale golden yellow color. Honeyed toast, spice, and caramelized nut aromas follow through of a soft entry to a dry-yet-fruity light-to-medium body with a tongue warming white pepper and wet stone fade."
$16.99 / 750ml Bottle
$193.69 / Case of 12
$193.69 / Case of 12
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Agency won't comment on what data may be in stolen computers
Atlantic Canada Opportunities Agency logo. File photo
Four laptop computers are missing from a federal government agency in Charlottetown, but no one is commenting on what data might be in them.
Staff at the Atlantic Canada Opportunities Agency at 100 Sydney St. in the city discovered the break, enter and theft on Saturday. The office had been "rummaged through," said a report from Charlottetown Police Services.
The matter remains under investigation, it said.
The Guardian contacted ACOA, asking what kind of data might have been on the computers, if any private or sensitive business data from clients or human resource was on the computers. The Guardian also asked what general policies and procedures guide and govern ACOA staff regarding data storage and what technical procedures are in place to protect data.
"Regarding your inquiry this afternoon about the police report, it would be inappropriate for the Agency to comment on this matter which is still under investigation," said an email to The Guardian Wednesday from Cindy Roy, director, communications, ACOA PEI and Tourism.
Anyone with information is asked to contact police at 902-629-4172 or anonymously to Crime Stoppers via SMS by sending "TIP162" plus your message to (274637) CRIMES, or by the web HERE, or toll-free phone to 1-877-222-8477.


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NEW YORK (AP) - There will be a different type of Sandman featured at the Apollo next month.
CAIRO (AP) - Egypt's ousted leader Hosni Mubarak, wearing a white shirt and loafers while flashing a smile, was released from prison Thursday and transported to a military hospital in a Cairo suburb where he will be held under house arrest.
FORT HOOD, Texas (AP) - American soldiers deploying overseas to "engage in an illegal war" provoked the deadly shooting rampage at Fort Hood in 2009, the soldier accused in the attack said Wednesday after refusing to mount a defense during his trial.
ALBUQUERQUE, N.M. (AP) - Gay couples rushed to a courthouse in Las Cruces on Wednesday after the county clerk decided to issue same-sex marriage licenses in a surprise move that came as several legal challenges on the practice make their way through the courts.
FORT MEADE, Md. (AP) - Bradley Manning plans to live as a woman named Chelsea and wants to begin hormone therapy as soon as possible, the soldier said Thursday, a day after being sentenced to 35 years in prison for sending classified material to WikiLeaks.
GRANTS PASS, Ore. (AP) - Running out of money to fight wildfires at the peak of the season, the U.S. Forest Service is diverting $600 million from timber, recreation and other areas to fill the gap.
HONOLULU (AP) - A German woman who lost her arm in a Maui shark attack has died. According to a statement from her family released by Maui Memorial Medical Center, 20-year-old Jana Lutteropp died Wednesday. Lutteropp's death comes one week after a shark bit off her right arm while she was snorkeling off Makena. Her arm wasn't recovered. The statement from her mother and sister says, "Jana fought hard to stay alive" but "lost ...
FRESNO, Calif. (AP) - An out-of-control forest fire threatening about 2,500 structures near Yosemite National Park was one of more than 50 active, large wildfires dotting the western U.S. on Wednesday.
WASHINGTON (AP) - For the first time since 2009, previously occupied U.S. homes are selling at a pace associated with a healthy market.
YORBA LINDA (AP) - In the hours after President Richard Nixon delivered his first major national address about Watergate, two future presidents called him to express their private support, according to audio recordings released Wednesday.
WASHINGTON (AP) - Almost a third of this year's high school graduates who took the ACT tests are not prepared for college-level writing, biology, algebra or social science classes, according to data the testing company released Wednesday.
FORT MEADE, Md. (AP) - Army Pfc. Bradley Manning was sentenced Wednesday to 35 years in prison for giving hundreds of thousands of secret military and diplomatic documents to WikiLeaks in one of the nation's biggest leak cases since the Pentagon Papers more than a generation ago.
WASHINGTON (AP) - The Obama administration, undertaking a major review of U.S. relations with Egypt, edged closer to a decision Tuesday about curtailing some of America's $1.5 billion in annual aid after the Egyptian military's crackdown on supporters of ousted President Mohammed Morsi.
LONDON (AP) - One Direction has one less eligible bachelor. Band member Zayn Malik is engaged to Perrie Edwards of the group Little Mix. They're both 20. Edwards was spotted wearing a diamond ring Tuesday at One Direction film premiere "This Is Us" in London. Edwards' mother, Debbie, confirmed Wednesday that her daughter and Malik were engaged. She told Real Radio: "It's true. They got engaged on Sunday and it's absolutely lovely." Columbia Records ...
FORT HOOD, Texas (AP) - The soldier on trial for the 2009 shooting rampage at Fort Hood rested his case Wednesday without calling any witnesses or testifying in his own defense.
CUPERTINO, Calif. (AP) - For the first time since introducing the device that changed cellphones forever, Apple will offer two distinct versions of the latest iPhones - a cheaper one made of plastic and another that aims to be "the gold standard of smartphones" and reads your fingerprint.
HELENA, Mont. (AP) - A newlywed wife with doubts about her 8-day-old marriage confessed she pushed her husband face-first off a cliff in Glacier National Park, then lied about his death and told authorities he had driven off with friends, court documents said.
BUENOS AIRES, Argentina (AP) - Longtime favorite Thomas Bach was elected president of the International Olympic Committee on Tuesday, keeping the powerful sports body in European hands.
WASHINGTON (AP) - Pushing military might and raising hopes it won't be needed, President Barack Obama threw his support Tuesday behind a plan for U.N. Security Council talks aimed at securing Syria's chemical weapons stockpiles, even as he continued to advance the fallback idea of U.S. airstrikes against Bashar Assad's regime.
REDDING, Calif. (AP) - A wind-whipped, fast-moving wildfire in Northern California's Shasta County damaged 30 buildings, many of them homes, and threatened another 350 structures, fire officials said Tuesday.
LAKE MARY, Fla. (AP) - The 911 call seemed frantic: George Zimmerman's sobbing estranged wife said he was threatening her and her father with a gun and she feared for her life. Please come.
NEW YORK (AP) - Former "Jersey Shore" cast member Mike "The Situation" Sorrentino realized he had an addiction problem on a trip to Australia in February 2012.
LOS ANGELES (AP) - He said he would eat a bug to get you to buy a car. He promised that he would even stand upon his head until his ears were turning red if you would just, "Go see Cal."
PARIS (AP) - The world's largest solar-powered boat has docked on the banks of the Seine River, its final port of call after a three-month voyage across the Atlantic Ocean to study how the Gulf Stream and climate change could influence each other.
AMSTERDAM (AP) - A painting that sat for six decades in a Norwegian industrialist's attic after he was told it was a fake Van Gogh was pronounced the real thing Monday, making it the first full-size canvas by the tortured Dutch artist to be discovered since 1928.
JOHANNESBURG (AP) - Richard Van As, a South African carpenter, lost four fingers from his right hand to a circular saw two years ago.
SAN FRANCISCO (AP) - Apple is expected to unveil its latest take on the iPhone Tuesday during an annual ritual that will probably cast a spotlight on the gadget maker's drive to regain market share and its sluggish pace of innovation.


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People Like Us premiere Page 1
Blake Lively, Coco Rocha, & More Grace The Red Carpet
So many fancy red carpets last night! Blake Lively, Salma Hayek, and more attended the premiere of Savages in Los Angeles, while Elizabeth Banks and Coco Rocha showed up at the screening of People Like Us, and Tika Sumpter graced the red carpet for the premiere of Madea's Witness Protection in New York. But which coast had the best dressed lady? Let's dissect the looks.


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DIY WordPress Theme Framework Part 1: Defining Your Needs
Tutorial Details
Platform: WordPress Estimated Time: 5 minutes Difficulty: Beginner - Intermediate
- DIY WordPress Theme Framework Part 1: Defining Your Needs
- DIY WordPress Framework Part 2: Creating the Theme
- DIY WordPress Framework Part 3: Using the Framework as a Child Theme
- DIY WordPress Framework Part 4: Using the Framework as a Boiler Plate
One of the best things about my education at the University of Scranton was the recurring lesson we learned about reuse. Reuse is incredibly important in programming for many reasons: easier testing, saving time, the ability to focus on more advanced things, etc. Once I graduated and entered the wonderful world of full-time freelancing, I decided if I was going to keep doing WordPress work, I would need to apply those reuse lessons to my everyday life. Number one on my list was a simple WordPress theme framework.
The first article in this series is going to be a little different from other ones on this site. In this article I want to communicate to you some of the lesson I learned about reuse and defining your needs. I will be providing my framework to you in the next article, but it's a fact that everyone codes differently and what I provide might not suit your needs best!
Some Principles of Reuse to Keep in Mind
Anyone with formal education in Computer Science or Software Engineering can attest to the fact that there are countless theories, libraries, and classes (programming classes, not school classes) dedicated to some very advanced forms of reuse and generic programming. While I think those are overkill for a theme framework, there are some principles I tried to follow when creating mine:
"Testing will ensure your code works before implementing it 5, 10, or 20 times."
- Design Your Code: I know when talking about WordPress, design usually implies the front-end design, but it's just as important to design the code. Lay out what your functions, classes, and pages will be before you start to code them up.
- Generalize When Possible: Possibly the most important principle is to recognize when you're reusing code snippets and to generalize them into functions. This will make managing and updating your code much, much easier.
- Document and Test Thoroughly: This is something you should do with all code, but especially code you plan on reusing frequently. Documenting will help you remember what you were thinking 6 months or a year down the line. Testing will ensure your code works before implementing it 5, 10, or 20 times.
Define Your Needs
Now that we've laid out some things to keep in mind, we need to define what we want our framework to accomplish. Remember each of us has our own needs, and while I'm going to talk about mine, yours may be different. My needs were fairly simple at first: I wanted a simple framework that did my initial work for me.
While creating WordPress themes for my clients, I noticed my process was the same: copy K2 (the default theme at the time), remove the stuff I didn't want to use, replace it with my code. A lot of my code was similar: the same CSS reset, the same CSS structure, the same style header, navigation, etc. After a while, I found it was easier just to copy my last client's theme and build off of that. It was then that I decided to build my own framework.
"We need to define what we want our framework to accomplish"
With that decided, I needed to define my needs: what was I doing over and over again, and what could I generalize. My list of requirements was as such:
- Plugable CSS: There are several parts of my CSS that rarely changes. This includes in WordPress class definitions, my CSS reset, some general classes I use (.hide, .left, .right, .clear, etc), and (usually) my IE Fixes. If I could abstract that all away, all I would need to do is dump in a site specific CSS file (called master.css after Dan Cederholm's simple CSS framework) and I know everything else would work properly.
- Constants for the Theme URL and Image Paths: These are 2 variables I need with every theme. If I could easily define them somewhere, I wouldn't need to worry about replacing the URLs for every site I create.
- Common WordPress Functionality: This is the menus, sidebar definitions, and anything else I could think of that I'd be typing over and over again.
- Generally Defined Template Pages: The common theme pages (header, footer, index) with enough on them to make them useful, but not so much on them that I'd have to really change theme every time I developed a new theme.
- Common Folders: I always have an images folder, css folder, and css/img folder. I had to include these too.
- Lightweight: It's got to be lightweight. I don't want to have to sift through pages and pages of code to find what I want. My though is that WordPress itself is a complex framework; why build a second complex framework on top?
I also wanted to build some function for features of several pages, like the "page not found message" and the posts page navigation. This goes back to the mentality that a single function will help me more quickly change multi-page features.
See What's Already Out There
The great thing about open source software is that if you need something, it's probably already done. The same thing goes from Frameworks. There are dozens of frameworks out there, so before you go off to develop your own, it would definitely be worthwhile to checkout what's already there. The purpose is to save time, right? No better way to save time than to not have to develop at all!
Thematic by ThemeShaper is a very popular framework
To help you out, I've compiled a short list of frameworks I've at least looked at (though most of them I have actually used):
While exploring these frameworks, keep your requirements in mind. Test these properly: download, install, enable. Then try building a child theme and playing with the settings. See what you find. Then make your decision.
For me, I felt the ones I tried were too complex and not lightweight. They are great for people who need a theme quickly but as far as customizing goes, I had to learn a whole new system in order to build themes. Like I stated earlier: I already know WordPress' complex framework/API. I'd have to replace that knowledge with another theme's framework/API. I decided to build my own, which would still use WordPress' functions, not replace them.
I'm sure you could have guess at the outset of this article that the inevitable conclusion would be that we build out our own framework. We've got our principles and requirements, and we've done our research. We're ready to build a framework, which we will do next time!
The last thing I invite you to do is chime in on what I've said here: what are your principles and requirements. Do you use one of the frameworks I've mentioned here, or have you already developed your own? Let us know!
In the next session in this series, we'll be applying these principles to the first day of work on our new framework!
- http://teerapuch.wordpress.com
- http://www.jodiwarren.com
- http://www.jodiwarren.com
- http://www.nomad-one.com
- http://flashjourney.com/
- http://www.960development.com
- http://gorelative.com
- http://www.bosonto.com
- http://atinder.com
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- http://samcohen.net
Pingback: Создаем фреймворк темы для WordPress своими руками: определяем цели (ч.1) | Wordpresso
- http://www.webdesignlift.com
- http://www.netprofitmarketing.com
- http://twitter.com/rpdtweet


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This fine vintage Elizabeth II sterling silver presentation dish has a plain circular form in the Art Deco style.
The dish is supported by a collet foot.
This fine dish is plain and unembellished.
This vintage dish / bowl is a very good gauge of silver, fine quality and in excellent condition.
The dish has been professionally polished and is in presentation condition.
Full hallmarks struck to the surface of the foot are very clear.
Reflections in photography may detract from the true representation of this item.
Height 7cm/2.75"
Diameter of body 18cm/7.1"
11 troy ounces/ 342g
To see this fine sterling silver bowl displayed in motion, please click on the play button at the bottom left of the screen above.
To enlarge the player please select the icon.
Further details on how to use the YouTube Player
To purchase this item online, click the 'add to cart' button. You will be clearly directed, step by step, through the purchase process that will enable you to buy via your selected payment method, directly from our website.
Sterling Silver and Ivory Handled Bread / Fruit Dish - Art Deco Style - Antique George IV
Price £1,295.00
Sterling Silver Fruit Dish - Art Deco Style - Antique Edward VIII
Price £675.00
Sterling Silver Bowl - Antique Edwardian
Price £595.00
Sterling Silver Presentation Bowl - Antique Victorian
Price £795.00


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We said a tearful farewell to Matt on Wednesday. Though things ended on a positive note, even therapists need encouragement. What advice do you have for Dani?
Necessary Roughness facebook page
You have been as valuable to that team as anyone who has come through.
First Look: ABC's 'Killer Women' Credit: (c) ABC, 2013 Part 3
Marc Blucas stars as Agent Dan Winston
First Look: ABC's 'Killer Women' Credit: (c) ABC, 2013
Of all the notorious lawmen who ever patrolled the violent Texas frontier, none are more storied than the Texas Rangers. Being the only female ranger in this elite squad isn't going to stop ballsy, badass Molly Parker (Tricia Helfer). Molly is committed to finding the truth and seeing justice served. While she's surrounded by law enforcement colleagues who want to see her fail, including Police Lieutenant Estaban Salazar (Vic Trevino), the Rangers have her back, led by Company Commander Luis Zea (Alex Fernandez). Molly's also got her brother Billy (Michael Trucco) and his wife Nessa (Marta Milans). On the verge of getting divorced from her smarmy husband Jake (Jeffrey Nordling), Molly begins an affair with sexy DEA agent, Dan Winston (Marc Blucas).
Killer Women stars Tricia Helfer (Battlestar Galactica) as Molly Parker, Mark Blucas (Buffy The Vampire Slayer) as Dan, Marta Milans (Shame) as Nessa, Alex Fernandez (Transformers: Revenge of the Fallen) as Luis and Michael Trucco (Battlestar Galactica) as Billy.
Killer Women was written by Hannah Shakespeare and is executive produced by Sofia Vergara (Modern Family), Martin Campbell, Ben Silverman, Luis Balaguer, Electus, Latin World Entertainment. The pilot was directed by Larry Trilling. Killer Women is produced by ABC Studios.
First Look Photos: ABC's 'Killer Women' starring Tricia Helfer, Michael Trucco, & Marc Blucas
Come next television season, Sofia Vergara will be pulling double duty for ABC, continuing to star on Modern Family but also executive producing a new drama, Ki
Marc Blucas estara en una serie basada en ago que escribio un argentino??!! wow
Season 3


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There is a slim chance that I may be dying. I am thoroughly convinced.
That sudden burst of adrenaline when you find out a bit more about yourself than you knew before.
I love that feeling.
In love with American Horror Story. Honestly I'm overwhelmingly in love.
How can you complain about how no one loves you, yet when I tried to give you all of my love you shut me down?
my biggest fear is losing touch with who i really am
wtf i got my nipple pierced.
I don't like you or love you or need you. I never really cared about you all that much. But that doesn't change the fact that you're in my dreams every.single.night.
that awkward moment when you try to correct someone and you end up being wrong okay i need to crawl into a whole and die
Ill never understand why people are afraid to hurt, to love, to cry, to be helpless, to scream, to fall for, to depend on, or to rely on. The list of things we are afraid to do can go on and on. But, ill never understand it. Those things may not be positive. However, they are a part of what makes life beautiful. Embrace those moments.
I just asked a girl out on a date for the first time in my life ahhhh


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Joe Wolverton, II, J.D.
Stand with Rand 2.0? Sen. Paul Considers Filibuster of Syria Vote
Senator Rand Paul (R-Ky.) told reporters Tuesday that he considering a filibuster of the vote to attack Syria.
Sen. Graham Will Face Constitutional Challengers in Primary
As he calls for an "aggressive U.S. strike in Syria," war drum major Senator Lindsey Graham (R-S.C.) is facing a battle for re-election at home in the Palmetto State.
DNI Clapper: We'll Tell Tumblr How Many Americans We Monitor
On his official Tumblr blog, DNI James Clapper says he will soon declassify documents revealing how many Americans are currently under surveillance.
Colorado Sheriffs Stand Strong in Defense of Second Amendment
GOP gubernatorial candidate Tom Tancredo reports on the efforts of more than 50 sheriffs to oppose the recently enacted gun control laws in Colorado.
Kucinich: Strike on Syria Makes U.S. "Al-Qaeda Air Force"
Former representative Dennis Kucinich warns that an airstrike on Syria would convert the U.S. military into al-Qaeda's air force.
NSA Surveillance in Germany May Threaten Merkel Reelection
Challengers to German Chancellor Angela Merkel are accusing her of permitting the NSA to conduct illegal surveillance on the people of Germany.
Fed. Judge Rules Oklahoma Challenge to ObamaCare May Proceed
A federal judged ruled that a challenge to ObamaCare filed by the Oklahoma attorney general may proceed.
Maine GOP Members Resign Over RNC Rules Manipulation
After being rejected by the RNC Rules Committee, seven members of the Maine GOP resigned, citing the RNC's decision to silence challenges to its hegemony.
Fed Judge Declares Anti-Gay Sermon a "Crime Against Humanity"
In a 79-page opinion, U.S. District Court Judge Michael Ponsor ruled that a case against a pastor for opposing homosexual relationships could proceed.
Ruling Reveals NSA Lies to Courts, Congress About Scope of Surveillance
A FISA court ruling from 2011 reveals that the NSA routinely behaves unconstitutionally and lies about it to the courts, Congress, and the public.


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On the eve of the 100th anniversary celebration of Fenway Park, Felger and Massarotti discuss the planned ceremony and also ask whether Fenway should still be the home of the Red Sox.
Mazz sill thinks it should be, because "its unique – all these [other stadiums], a lot of them, are the same. They're all built the same way now."
Felger questions the wisdom of the celebration and the hoopla surrounding it, comparing the celebration to a cocktail or a wedding, with bickering over the invitations and the planning of the event.
It is, according to Felger, "another thing that has nothing to do with the actual game…it is just one thing on top of the next."
Felger and Mazz then turned to the current incarnation of the Red Sox under Bobby Valentine. According to Mazz, "This thing is a train wreck." What has led to the team's dismal start? Of Valentine, Mazz says, "he doesn't look like he can manage a game."
Has Valentine 'lost it'? Felger and Mazz discussed the possibility. Should Red Sox fans be worried?
What about the possibility of Valentine stepping down during the season? One caller asks this, and Felger and Mazz end the segment by giving their opinion.


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The 2012 version of Kansas City's Restaurant Week features over 125 restaurants from all over the city. This weekend someone was asking me what restaurants are involved and which ones I'll be hitting up myself. This post is a list of the participating restaurants. Later in the week I will post about some of the individual establishments, their restaurant week menus, and other fun stuff.
So now to the list:
For more info on Restaurant Week visit KCRestaurantWeek.com
Bistro hereford | Joininghandsca (1 year ago)
[...] 2012 KC Restaurant Week Participants Comments Off [...]
Imogene (1 year ago)
Shopping app fans that use an Android or iTunes might want to appreciate a free one I found titled The Coupons App. This weekend get a 40% coupon at Elder-Beerman. $10 off coupon at King's Discount Stores. A FREE plate coupon at Schlotzsky's and an appetizer coupon to Shoney's! http://thecouponsapp.com/download Seriously, can't go wrong it's free.
Ellen (1 year ago)
Daily deal app lovers that use an Android or iPhone might want to try The Coupons App. Tomorrow get a 70% coupon to Nordstrom. $15 off coupon at Nordstrom Rack. A FREE entree coupon for Ponderosa/Bonanza Steakhouse and an appetizer coupon for Shoney's! http://thecouponsapp.com/download Honestly, free samples.
Nichelle (1 year ago)
Discount app users on Android or iPhone oughta download this free one called The Coupons App. Open it and there is a 70% coupon for Carson Pirie Scott. $10 off coupon at Palais Royal. A FREE combo meal coupon at Chronic Tacos and an appetizer coupon at Souplantation and Sweet Tomatoes! http://thecouponsapp.com/download Seriously, see for yourself.


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The number of Facebook users is rapidly approaching one billion – and this despite the fact that the site is blocked in China. Yesterday the company issued a correction to the shape of S1 in anticipation of a public offering. The new document provides fresh statistics for March 2012.
In March, Facebook reached 901 million people – the ones who went to the site at least once a month or for a specified period of time fixed by pressing the Like, and tracking cookies. Daily active audience in March amounted to 526 million people – many fixed tracking network Facebook daily. Around 500 million people a month use Facebook mobile application.
Every day, social network users leave 3.2 billion "Likes" and comments and published 300 million photos. The site recorded 125 billion "friendly relations" (on 31 December 2011 was 100 billion).
When Facebook filed an initial application for an IPO in February, the monthly audience of January stated in 845 million. Thus, we can estimate the growth of social networks: 56 million people in two months (6.6%). Perhaps, Facebook will cross one billion milestone before the end of the year: around August.
Of course, Facebook is now the world's largest social network. In second place is Twitter, which in February stood at 500 million accounts (although, of which only 140 million are active users).
Other numbers: the company's revenues in the I quarter. 2012 amounted to 1 billion $ 58 million, which is 6.5% lower than the previous quarter. So, each user has earned the company a little more than a dollar in the quarter. Advertising has brought $ 872 million profit, and other sources – $ 186 million.
The document also pointed out that Facebook has just purchased a package of 650 Microsoft patents at $550 million, which it recently bought AOL. Now Facebook has quite a strong defense of the more than 1,400 patents.


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This free real estate calculator for Windows helps you determine whether purchasing a home or renting is your best option. Free Rent or Buy Calculator is a real estate calculator designed for Windows 95, 98, ME, NT, 2000, and XP systems. It offers integrated help, provides automatic hints, and offers Wheatworks' integrated Upgrade Center for easy, online updates.
Platform:Windows 95, Windows 98, Windows Me, Windows NT, Windows 2000, Windows XP
System Requirements: There is no specific requirements


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J.R. Dill got his start 8 years ago making wine as a hobby in his parent's basement. Things progressed from there and in 2009 founded J.R. Dill Winery. J.R. Dill's great grandfather, H.J. Dill worked in the vineyards here over 100 years ago. (Pictured left, far right).
J.R. Dill invites you to come & enjoy our wines and enjoy the atmosphere. The winery tasting room features early 20th Century photos of Watkins Glen and Seneca Lake, and a unique tasting bar made of Seneca Lake glass! Come experience first hand J.R. Dill Winery.
J.R. Dill Winery is located six miles north of Watkins Glen on the east side of Seneca Lake on New York State Route 414. We are on the righthand side of the road if you are traveling north. Once you see the Seneca Spring Resort we are the next driveway on the right. If you are traveling south on NYS Route 414 we will be on the left about 1/2 mile past Atwater Estate Vineyards. Need more info? Please call us at 607-546-JRJR
Mon - Sat: 11 am to 6 pm
Sun: 11 am to 5 pm
Mon - Thu: Closed
Fri-Sun: 11 am to 5 pm


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UF Student Praised For Quick Thinking
Published March 5th, 2010
University of Florida Police are looking for a man accused of possibly stalking a student on Thursday.
A female UF student said while she waited for a bus, she was approached by a man asking for directions to the Reitz Union. But, the man followed her onto the bus, which was not heading towards the Reitz Union.
Police say the man was taking pictures of the girl and got up to leave when she did. She ended up staying on the bus after the man left on the final stop, and then called friends to pick her up.
The man's described as Indian, approximately five-foot six-inches tall with black hair and brown eyes. He was wearing dark colored pants, possibly jeans and a jacket.
Related Stories
- Dog Trainer Teaches Humans to Think Like Animals
- Sunscreen May Be More Important Than You Think
- Thinking Inside the Box: Innovative Construction in Gainesville
- Ghoul, goblin, skunk ape? Think again, says FWC
- Quick Response Saves Life- Earns Honor
- Quick Errands Could Lead To Deadly Consequences
- "Quick Change" Fraud in Gainesville
- 2/4/13 Fitzness Minute: Quick Cardio Workout
- The High Price of Quick Loans
- Trayvon's Mother Thinks Shooting was an Accident


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Trintech History
Since 2000, Trintech has focused on its distinctive competence — developing integrated financial software solutions that make a strategic difference to businesses everywhere.
Trintech has nearly 700 leading customers in dozens of industries including the commercial, financial services and healthcare industries.
Cadency, by Trintech, is the world's only end-to-end financial governance solution that manages the entire Record-to-Report process in a single product. With Cadency, the Office of Finance can finally establish a repeatable rhythm for the Record-to-Report cycle. All stakeholders can be orchestrated and all process activities streamlined while measuring and managing the risk profile in the financial governance model.
Trintech was acquired in December 2010 by Spectrum Equity Investors. This acquisition allowed Trintech to accelerate the growth of its financial automation solutions, positioning the Company to execute on its global expansion strategy. Following the acquisition, Paul Byrne was appointed Chief Executive Officer.


text: More Than Special! More Than Unique! More Than a Step Back in Time! Place a Visit to Whitehall Bed and Breakfast on your Bucket List! My husband and I discovered Whitehall B & B a few years ago when we were in Madison for the Historic Christmas Candlelight Tour of Historic Homes. Since then, we have not missed our annual... More


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Make your reservations now by using OpenTable.
Make a ReservationSpacious dining rooms high atop the misty Blue Ridge Mountains feature the season's harvest of fresh local ingredients at The Restaurant at Chateau Morrisette. Sensitive to the preservation and prosperity of our rich cultural environment, the restaurant offers southern regional cuisine in an atmosphere of rustic elegance. From simple entrees to complex appetizers and creative desserts, our food is prepared to create the ultimate culinary experience. Expertly paired with our award-winning wines, the restaurant's mission is to inspire your return and create warm memories.
Recently refreshed and refined, our dining rooms offer casual and leisurely dining whether for impromptu get togethers or special occasions. Innovative wine cocktails have been added to our bar, a cheerful location to meet friends. New bistro tables and stools are also a fun seating alternative in the lobby area. Everyone anxiously awaits warm weather and dining on our outside deck. Cheerful umbrellas shade diners from the sun while drinking in the mountain vistas.
Reservations are suggested and can be made on line by using the link on this page or by phoning 540-593-2865. We are easily accessible for guests requiring special equipment and children are especially welcome to accompany any party.
Please note that parties of 6 or more must be billed on one check and a 20% gratuity is automatically added to your guest check.
Cancellations of reservations requested 24 hours in advance. Please note that tables can only be held twenty minutes after the scheduled reservation time. Kindly give us a courtesy call if you are running late. For your health and ours the entire restaurant and dining patio is non-smoking. Smoking is permitted in outside designated areas only.
Dress Code
We want you to enjoy your dining experience with us, CASUALLY, with our liberal dress code. Be comfortable, relax and let us serve you.
Weddings and Special Events
We welcome weddings, wedding receptions, birthday/anniversary parties, business meetings, reunions, and other special events.
For business meetings, corporate events, reunions, weddings, wedding receptions, and other special events, please contact our event co-coordinator, Sally Truslow, at 540-593-9223 or firstname.lastname@example.com.
For birthday or anniversary parties, please call our restaurant manager, Misty Tunstall, at 540-593-9236 or firstname.lastname@example.com.
Complimentary Winery Tour Schedule
Monday - Thursday | 11 am, 1 pm & 3 pm |
Friday & Saturday | 11 am, 1 pm, 3 pm & 5 pm |
Sunday | 12 pm, 2 pm & 4 pm |


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Torture and lack of sanitation in the Transnistrian imprisonments
Human rights in Transnistria are a sensitive subject. A Promo-LEX report shows that the inmates from the left bank of Nistru River of Moldova experience serious human rights violations.
The persons arrested by the so-called "local police officers" of the separatist region are subject to various forms of torture in imprisonments, the perpetrators being forced to bear the necessary testimony. The most used form of torture is sitting the person on an iron chair, bandaging them and pulling their hands causing injuries and pain.
The psychological torture is another method. The victims are blackmailed and put together with those who carry infectious diseases and have no access to the necessary health care.
Alexandru Zubco, attorney at Promo-LEX Association, told Moldova.ORG that there isn't a national programme to help the victims recover after they have been imprisoned in Transnistria.
"There are not rehabs for the victims of the regime from Tiraspol. The relatives of the victims themselves who denounce the actions of these illegal structures to the constitutional institutions, are frequently threatened, intimidated and stalked," said the author of the report.
The document provides the story of some inmates and their relatives. According to them, Tighina imprisonment consists of a basement of a building without daylight. The only possibility to air the cells is when the food arrives. There are also common toilets and bathrooms, and the men and women have to shower in the same space.


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I don't want this community to die... I want to keep it updated hopefully. All Sakkan fans are precious.SPIRAL SPIDERS GOT BACK TOGETHER!As a 5 member band for now, but right now their replacement drummer is "Takoyaki Boy." He's performed with them 5 times so far.SPIRAL SPIDERS links:TwitterMyspace (same as before, but updated)WebsiteYoutube (new)You can find live photos from their 3 night one-man live series ("one-man" means it's only them performing) here:flickr photosI believe that Kingfisher Jr. is over. It hasn't officially been said, but now that Yosuke is busy with SPASPA again I can't see him and TOMI YO continuing it. RADIO FLOWER is also over (it ended in January), but Yosuke is apparently coming back on ustream in June. Sora Izumikawa of SPIRAL SPIDERS is restarting her old radio show Soradio on ustream at 10 pm Thursday (Japan time) here. Be sure to tune in! Sora also has an official twitter and a twitter for her radio program.The concerts that SPIRAL SPIDERS have been doing have been a secret live in February, the 3 night only one-man series of lives in March (in Tokyo and Osaka), and just this past weekend at an Antinos 10 year anniversary concert with Kinya Kotani.Lastly,ENJOY A BRAND NEW PV FROM SPIRAL SPIDERS! (not for sale on iTunes or anything yet)x-posted to supa_supa
kingfisher jr. has an online broadcast from 20:00 ~ 21:00 JAPAN TIME every Friday, and you can see it on their myspace, so be sure to tune in!The very first broadcast is July 11th (Japan time. May be earlier in your location).There is also a CHAT ROOM that you need an account to log in to with Ustream.If you've never participated in one of these before, with an account, you can type a message to the musicians and they'll read it instantly and you can see their reactions/responses right away on-screen.
If you're still in the community, I think this'll make you guys very happy :).New FOLK/ELECTRONIC/POP band, Kingfisher Jr.TOMI YO + guess who?http://profile.myspace.com/index.cfm?fuseaction=user.viewprofile&friendid=1001234795
Happy Birthday Takashi!Back in October, I requested a translation of ブライス through a Japanese music translation project website called "Mognet." I've come to realize Buraisu is "Blythe" (as in the dolls). I translated a very in-depth description of CALL, including Buraisu, which said the song is as if it's in the point-of-view of a Blythe doll, with its huge eyes, and looking at everything wrong with people. 人は悲しい生物だ!I didn't post about it before, but this summer I had the opportunity to hear Buraisu/Blythe live, and it is absolutely maaaniic.I just got the translation today. If you have the original kanji (in CALL's booklet or whatever), feel free to point out any errors :0. My Japanese skill is still too limited to make any sense of Yosuke, so that's why I'm too wary of doing this myself. I'd like to think he's weird anyway, though.( buraisu/blytheCollapse )
Just wondering if anyone has MySpace?Spiral Spiders now have a MySpace page.http://www.myspace.com/spaspaThey want more friends, so add them!


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April 25, 2010 Dems seek to gut Citizens United SCOTUS decision
Respect for the Constitution? Respect and deference to the Supreme Court of the United States? That was so 2008 and before. We know how Barack Obama has a fetish for attacking groups, including the Supreme Court justices during the State of the Union address.
Now we can witness moves afoot by Democratic Senators to try to weaken their decision in the Citizens United Case - the decision that allowed corporations to fund independent political broadcast in elections. Most Senators are lawyers; but here political power and the desire to help control what citizens hear takes precedence over respect to the Constitution. The White House is working with key Democrats to weaken the impact of Citizens United. Meanwhile, unemployment stays stubbornly high-and our politicians are spending time on figuring out how to chill free speech,.
The legislation will address seven major points: * Enhance Disclaimers: Make CEOs and other leaders take responsibility for their ads. * Enhance Disclosures: It is time to follow the money. * Prevent Foreign Influence: Foreign countries and entities should not be determining the outcome of the elections * Shareholder/Member Disclosure: We should allow shareholders and members to know where money goes. * Prevent Government Contractors from Spending: Taxpayer money should not be spent on political ads * Provide the Lowest Unit Rate for Candidates and Parties: Special interests should not drown out the voices of the people. * Tighten Coordination Rules: Corporations should not be able to "sponsor" a candidate. Of course, the devil is in the details. For example, "provide the lowest Unit Rate for Candidates and Parties" means that entrenched incumbents and political parties are weakening the chance that opponents will be able to compete with incumbents. Democrats are trying to make it easier to keep their seats - now that they are in the majority. Preventing government contractors from spending means that an entire group of businesses will not be able to engage in supporting free speech merely because they receive more than $50,000 in government money. In an era where government is coopting major sectors of the economy and spending money as if there is no tomorrow, this will foreclose the ability of many businesses from being able to express their political views. One could go on about this attempt to chill free speech. |
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text: I have an application that decodes MPEG transport streams and outputs scaled images to a framebuffer. It's running on a 2 GHz Core Duo, and there is some CPU power unused. Currently, with 1280x720 input images there is some choppiness at 1024x576 in the video, but not audio. There is none noticable at 512x288 (preview). From the demuxer, I have mpeg2dec, videoscale, ffmpegcolorspace, and my fbdevsink, separated by queues. The fbdevsink takes RGB, in this case 32 bpp, depth 24, and the transport streams decode to YV12 (or it can be I420). I don't see any hints what the relative performance is for nearest neighbor, bilinear, and 4 tap scaling. BTW, I have not been able to get 4-tap scaling to work, even with the colorspace conversion after the scaler (the fbdevsink color space property seems to be passed up to the scaler, which cannot scale RGB, so it refuses). Any links to help me choose between the scaling methods? If 4-tap would be best for me, any idea how to get the scaler to output YV12 to the color space converter (another filter element between the color space converter and the frame buffer sink??)? Thanks.


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- November 19, 2011
- 4:05 p.m.
- Suggest removal
- Reply to this post
Chicagoan writes:
Believe me...this had nothing to do with students being coddled or not behaving as adults. This guy has some serious mental problems. He didn't just get "snippy" with people, he could make co workers' and students' lives hellish with lack of communication, inappropriate outbursts, ridiculous expectations, and conduct that was completely unacceptable. I witnessed it first hand. To his credit, he is terribly talented and his designs are excellent. He just can not work with people. He is emotionally labile and will speak normally one moment, scream obscenities in the next moment and follow that with tears and regret at his outburst. This is more than an overdramatic theatre professor. This is a human being with deep seated problems who displayed horrid behavior over and over and over again. USI should be ashamed that they didn't act sooner...when student after student and concerned staff members voiced their dismay at this man's continued employment as an instructor. Everyone who worked with him and knew him felt as though they walked on eggs or were handling a live grenade when they were around him. No one deserved to be treated the way he treated people. He never did anything to me personally, but I was unfortunate enough to watch in amazement as USI ignored his behavior time after time after time. I know he apologized to this student...what about all the others, Robert? What about the ones who worked their hardest to do what they were supposed to do only to be treated horribly by you? What about the ones you treated so horribly that you brought them to tears? As a bystander I was appalled at what I saw. Shame on USI for not having higher standards. They are the ones afraid to offend someone...it's easier to look the other way I suppose.
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Twin Bridges Golf Club's 250-acre Robert Lohmann–designed course regales sphere slingers with picturesque views of the neighboring White Lick Creek and the emerald foliage of nearby copses. Holes on the front nine showcase large greens and fescue-lined bent-grass fairways, and the back nine rolls over hills with greens closely patrolled by sand traps, waterways, and burrowing Pacmen. This oasis of recreation remains open to the general public and features year-round play.
High above a lush vineyard, the morning sky brims with creatures of flight: an eagle, a large bumblebee, a pigeon that forgot how to land. These were just some of the sights Airbus Balloon Rides' owner Andy Richardson imagined would greet his future down-gazing passengers. He first fell in love with the roar of a hot air balloon's flame just before entering the second grade, and that passion has driven his dreams ever since. Ten years after buying his first balloon at age 14, Andy now commands a fleet of rainbow-colored balloons that come in standard and specialty shapes. These colorful vessels set the elevated stage for individual flights and tethered rides helmed by Andy and his talented team. Flights lift off at sunrise, in the afternoon, and at sunset, when the low sun paints an orange-red glow over water, fields, and reindeer still stuck on rooftops.
Back on land, Airbus Balloon Rides also educates visitors on hot air balloon creation inside their balloon factory, which welcomes tours. At the end of each tour, the guides lead guests in a champagne or mimosa toast with accompanying hors d'oeuvres, celebrating their skyfaring adventures together.
The eclectic organizers at Red Frog Events take a lighthearted and fun-focused approach to building their adventurous events, such as obstacle courses, scavenger hunts, and themed bar crawls, to connect city dwellers with local neighborhoods. Their creative, interactive offerings include regularly occurring competitions such as the Warrior Dash, Great Urban Race, and Beach Dash, the proceeds from which usually benefit St. Jude Children's Research Hospital. Like the idea of having a pet rock, their events have grown more popular annually, and frequently spring up in cities across the United States.
Looking for a fresh turn in their respective careers, Joe and Dawn Taylor planted the first grapes at Sleepy Creek Vineyards in 2002. In 2007 they opened the winery, where they sell their wines and swap stories with visitors. Amid 10 acres of expansive grapevines, a timber-frame barn houses the winery and tasting room. While sipping Sleepy Creek's varietals, guests can browse the gift shop, peruse the upstairs art gallery, and sample local cheeses from Ludwig Farmstead Creamery and Ropp Jersey Cheese. Above the winery, bed-and-breakfast-style accommodations allow visitors to relax amid the countryside and eavesdrop on rumors spreading through the grapevines outside.
Ryan Ketchum was overweight. When he decided to change his lifestyle, however, he held nothing back. In less than a year's time, he lost 110 pounds and became the athlete he'd always dreamed of being. His dedication impressed lifelong athlete and All-American track and field competitor Wil Fleming, and together they founded Force Fitness & Performance to help others experience similar success.
Now, joined by six other expert trainers, the founders help everyday citizens shed pounds with personal training and boot camps. They also help competitive athletes improve their performance through functional motion training, which cuts unnecessary movements such as lifting the knees too high when running or clicking one's heels when taking jump shots.
Function. That's what CrossFit is all about. And that's what got Shaun and Jenna Tieman so hooked on the method that they decided to open Hoosier CrossFit. During classes, CrossFit's system of constantly varied movements are performed at a high intensity. Because the movements are functional, they increase agility, speed, and power while readying participants for all manner of physical activity. Classes rely on tools such as gymnastics rings and bumper plates instead of machines, ensuring that the body is doing all the work. To initiate the uninitiated, instructors start all beginners out with On-Ramp classes, which familiarize exercisers with CrossFit basics.


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Little Rabbit is sad to see winter come. But his momma reminds him of how fun winter can be.


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Big Cuts with a Lightweight Design The Milwaukee 0721-21 V28 Li-Ion 1/2″ Right Angle Drill Kit is a lightweight 28-volt system with heavy-duty power capabilities. The Milwaukee V28 technology is engineered to be so powerful it easily cuts through 2 x 4 studs and delivers the weight of 3.0 amps per hour. The 28-volt battery is significantly less weight ...
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Milwaukee 0721-21 V28 Li-Ion 1/2″ Right Angle Drill Kit
June 3, 2013
Posted by Tool Up on June 3, 2013
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