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Lawrence Decker, mining king of Trinity and surrounding areas, has a job for you. There's a salvage site he wants you to dig up—and quickly. Time is definitely of the essence. Oh, and of course, there's possible danger.
"Trying Times in Trinity" is the first in a series of adventures to be offered for the Rogue Mage game. If you l... [click here for more]
The lowest ranks of the Darkness are the spawn, a nearly limitless horde of hungry and aggressive beings. They are the most commonly encountered creature of the Darkness and remain a constant danger for rural communities. Many of those traveling beyond the protection of cities invest in Charmed Circle amulets to ward off spawn they may encounter. Others simply disappear, lost to the ravenous ap... [click here for more]
Minor infernal powers are the weakest immortal powers of the Darkness; an intermediate rank between major powers and Dark mortals. Minor powers are often ambitious and seek to improve their position through service to their masters and the destruction of their rivals.
Creatures of Darkness Vol 2: Minor Powers is the second in a series of monsters to be offered for the Rogue... [click here for more]
Major powers of the Darkness are direct personal servants of the dragons. Some are major seraphs who fell (e.g., Reshep), while others are fallen minor seraphs (e.g., Forcas) who've grown in power over millennia. Major powers act as the de facto rulers of the Darkness, communicating the will of their bound masters to lesser servants. Some work feverishly to free their masters. Others enjo... [click here for more]
Opposing the seraphim are the powers of the Darkness, former servants of the Most High who rebelled against his rule, as depicted in Christian Scripture and apocrypha. After their defeat they were cast into Hell. Over the eons since then other servants of the Most High fell from grace, bolstering their infernal ranks.
Dragons are former major seraphs who participated in the original Fal... [click here for more]
Rogue Mage Creatures of Darkness Vol 5: Dark Mortals brings you a selection of new foes born of humanity's corruptible nature.
Beneath the minor powers in the infernal hierarchy are corrupted mortals and their hellhole-bred offspring. They are the foot soldiers of the armies of Darkness, and the infernal powers are always seeking to expand their ranks in preparation for fu... [click here for more]
It's a century after the Seraphs returned to Earth, fulfilling ancient scripture with fire and sword. Most of Earth's population died in the plague and resulting wars -- and then came the ice age.
Mankind has survived, but not without change.
Wondrous new races were born following the Time of Plagues, some destined to fight alongside the Seraphs of the Light, while others simply... [click here for more]


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Im looking for a latios willing to trade starters from any region all if you want and I can trade any of tms and mastetballs pm with offers
Im looking for a latios willing to trade starters from any region all if you want and I can trade any of tms and mastetballs pm with offers
Do you havre careful nature registeel shiny I have a latios
I'm looking for a legit latios. Dont care what nature. Preferably untouched, but flexible with that part. Willing to trade an EVENT Arceus, among other rares. PM me if interested.
I have a legit Latios that I cought on my Pokemon emerald, lv. 45. I was just courious what offers I could get for it. Pm me with your best offers, I have almost every legendary so I'm only really looking for shinies
Currently playing: Pokemon X
Shinies for trade: Adamant Pansear (with 2 max IVs from friend safari)
Add me for Pokemon Safari, trades and battles / FC: 3454-0767-8038
Friend Safari: Ghost type containing Shuppet, Phantump and Drifblim
Looking for 40+ Latios . Willing to trade many legendaries.
Pm me if Intrested.
Wanted: Shiny Speed or Attack Nature.
Many Pokemon to Offer and Trade.
TRU Arceus (Can Activate SS HG Event in Sinjoh Ruins)
Birth Island Deoxys UT
MYSTRY Mew
And Many More. (Too Lazy to Put it Here.)
My Friend Code Here:
[SPOILER=My Pokemon Teams] Spoiler:- -My ThunderStorm Team:
Looking for Latios serious nature any level I can trade almost any legendary....
Pm me
Latios/Lv 52/Quirky up for trade. PM if interested.
*Currently away from trading/games while finishing classes*
Diamd FC: 1636 3049 4944
SoulSilver FC: 2710 0881 6322
White FC: 5458 8254 3119
I wanna be the very best.
Have4th Gen >(UT)(UT)(crown)(crown)(Crown)(10 ANIV/UT)x2 (not HG)(not SS)(Movie Promo/UT)(UT)(UT)(event/JP), (UT)
5th Gen >x2, (UT)
Can Breed >(Dragon Dance & Outrage)(Hydro Pump)
Shiny >
Want4th Gen > n/a
5th Gen> (DW)(DW)
i am looking for latios for my pokemon platinum version. I give you exchange 100lvl pokemon very good training good natures,legendaries and other pokemon for pokemon white version.send me a message
Lv70 Relaxed nature || ALAMOS Event
x3 Lv50 Hardy,mild,bold nature || WIN2011 Modest flawless nature
x2 Lv50 docile,naughty nature || GAMESTP
x2 sassy,nasty nature || Oblivia
adamant flawless nature || TRU
Jolly flawless nature (MAY2010) || TRU
Shiny Event || TRU
Shiny Event || TRU
Shiny Event ||
Looking For any shiny(Prefer Pokeradar's)
i am looking for latios jirachi deoxys celebi and all shinies pm if interested with offers and what u want in return for them.
Can I have one I'll give a masterball in exchange as well.
i'm looking for a legit UT flawless timid nature latios PM/VM me for what u want in return
MY 3DS Friend Code: 1848-2888-8103 Safari- Pidgey, Woobat and Rufflet
My Black 1 FC Just for Trades: 2795-7384-8121
My Black 2 FC just for Battles: 5286-9239-4973
MY SHOP- http://www.serebiiforums.com/showthr...iny-Event-Shop
credit to FairyWitch for userbar
Looking for a ut bold latios can offer a lot of stuff pm me
My new team;
Diamond fc trainer name Erik: 0046 8727 1813
My shinies; looking for a shiny Aggron
I have claimed my favorite Pokemon of all
Trade completed!!!
Last edited by EXGShadow; 15th September 2012 at 3:27 AM.
White 2 FC: 3311 2771 3038 / 3DS FC: 0044 3638 4020
Looking for:
-Lansat, Starf and Enigma berries;
-Burn Drive, Shock Drive;
-Hidden ability Pikachu.
Looking for a Latios
I can offer a wide range of pokemon, mostly from Kanto and Hoenn, Such as Every Starter from Kanto,Johto and Hoenn.
I have a large list of trade-able pokemon, PM me with a list of pokemon you are looking for and I'll see if I can assist!
Currently using Pokemon Diamond!
Looking for Kanto, Johto, Hoenn and Sinnoh Legendaries!
No Shiny's up for trade At the moment! Sorry.
Pm me for Friend code!
Offering level 90 kyogre for latios if interested contact me on email@example.com or. If u have any jhoto legendaries I'm willing to trade as well
I have all regies PLus regigigas. Currently looking for darkarai Latios or Latias also willing to trade for any jhoto legendary of interested email me at email@example.com
I'm looking for Latios, I don't have much to offer, but I have a spare Cresselia, Zapdos, Articuno or Moltres, so if you have a spare Latios, PM me and we might work something out
Current shiny hunt: 1401 eggs hatched so far
HG FC: 1593-0713-2225, OT: Kofola
Black FC: 1464-3638-2370, OT: Rajec
Current HG Pokedex status: 360 owned
Hey, I'm Mike
I have a Latias, but I need Latios. I'll take either full trades or temporary trades to fill the pokedex. PM me if interested \m/
Looking for all starters, aside from Tepig.
Black FC: 4943 6914 2387
Looking for the duo, can offer a few Legendaries and some breedables, message me what you're after I'll see what I can do
I own Heartgold, Platinum and SoulSilver. I am breeding the first four gen starter Pokémon! Send me a message if you want one and I'll get it how you want it (apart from shiny, and I will want more if it's shiny!) I will also breed other Pokémon if you want Just PM me!
Legendaries For Trade!
Events For Trade!
Shiny Pokémon For Trade!
Interested in: Shinies (Offering legendaries)
People banned from trading/battling me: GiantsFan |
Looking for a Modest Latios, PM me if interested, check my signature for more info and i can also offer items, rare candies, pp max, pp up, ev trained, dw babies, and shinies.
Admnt, Mdst, Timid, Jolly Admnt, Mdst,; Admnt, Crful, Impsh, Mdst, Admnt, Mdst, Calm, Calm, any nat low Attk, Calm, Mdst, Calm.
Pokémon Y
Safari Friend:
Munna, Espurr and Xatu.
Looking for a Latios;
Preferably a Timid/Modest and Untouched one.
I can offer Lugia, Phione, breedable pokémon. PM me if interested.
White 2 FC: 3311 2771 3038 / 3DS FC: 0044 3638 4020
Looking for:
-Lansat, Starf and Enigma berries;
-Burn Drive, Shock Drive;
-Hidden ability Pikachu.
mewtwo will be equiped with powerlens, latios must be LV35-40 brave and named Zeo. pm me
I have an untouched Latios, level 40 available to trade. Will probably be cloned.
I am looking for legit (Cloned OK) at any level:
really? you do? is he brave?

subset: commoncrawl


text:
I don't think about my divorce often, anymore.
But when I do, it still fucking makes me cry.
Note to self:
Never hire Mlaw for anything cuz shes a jesus loving, right wing, conceited, jesus loving, pompous cake eater
We don't have kids. Obviously we can't predict the future but let's say things went south.. me and Mrs. Nantrax got a divorce... the only custody battles would be over the ferrets.
I think she is keeping me around for now. I'm getting a minor surgery/procedure this coming friday. It's a big deal and she said she wanted to be with me that day. And I expect her to stick around on saturday to make everything ok.
Get off my back, Babylon!
ok, here's what I found out---You can file the divorce yourself and then file a motion to appoint the warning attorney, he or she then has the responsibility to try and find your husband, if he can't he files a report. He is entitled to get paid but it should only run about $150.
gridman should hold a bake sale for ftm's attorney fees.


text:
Sort By: Descending Journal Year Ascending Journal Year
Feliciello, I., Parazajder, J., Akrap, I. and Ugarović, Ð.
Notes: GoTaq® Green Master Mix was used to amplify Tribolium castaneum satellite DNA that had been bisulfite treated to detect methylated cytosines. The bisulfite-treated satellite DNA was amplified using methyl-specific primers in a total volume of 30µl using 2X GoTaq® Green Master Mix, 2mM mix of the primer sets, and 1µl of the bisulfite modified DNA.
Expand Full Notes »
GoTaq® Green Master Mix
Farkas, T., Fey, B., Keller, G., Martella, V. and Egyed, L.
Notes: Mice RNA samples were converted to cDNA using an oligo-dT primer with the Reverse Transcription System, ethanol precipitated, vacuum dried and transfered to another lab. There they were reconstituted in 20μl of molecular biology grade water.
Detection of caliciviruses in the wild mice samples was attempted using generic calicivirus primers targeting sequences encoding conserved amino acid motifs in the RNA-dependent RNA polymerase (RdRp) region of ORF1. Two microliters of cDNA was used in 25μl PCR reactions using the GoTaq® Green Master Mix. Laboratory mouse RNA samples were tested only with MNV-specific primers in the AccessQuick™ RT-PCR System using 2μl RNA as template. PCR products were cloned into pGEM-T® Vector and sequenced using M13 forward and reverse primers on an ABI PRISM® 3730 DNA Analyzer. (4330)
AccessQuick™ RT-PCR System
pGEM®-T Vector System I
pGEM®-T Vector System II
Reverse Transcription System
Expand Products »
Ismail, N.A., Ragab, S.H., Elbaky, A.A, Shoeib, A.R., Alhosary, Y., and Fekry, D.
Notes: These authors investigated the differences in gut microbial flora between obese and normal-weight subjects. They used the Wizard® Genomic DNA Purification Kit to extract DNA from diluted fecal extracts. The extracted DNA was analyzed by PCR to identify Bacteroidetes and Firmicutes. Differences in distribution of these phyla was between the subject groups were identified. (4220)
Wizard® Genomic DNA Purification Kit
GoTaq® Flexi DNA Polymerase
Crawford, M.A., Lowe, D.E., Fisher, D.J., Stibitz, S., Plaut, R.D., Beaber, J.W., Zemansky, J., Mehrad, B., Glomski, I.J., Strieter, R.M. and Hughes, M.A.
Notes: The authors identified three genetic loci involved in chemokine-mediated antimicrobial effects against Bacillus anthracis using a transposon mutant library in which a transposon is randomly inserted into the B. anthracis genome, then treating the mutant cells with chemokine to select for resistant cells. To identify the transposon insertion site, and thus the resistance-conferring loci, the authors amplified regions flanking the transposon by PCR using the GoTaq® Green Master Mix. (4167)
Ruhlmann, J.M., Kram, B.W. and Carter C.J.
Notes: The authors used microarray analysis to identify genes that are differentially expressed in nectaries of Arabidopsis and may be involved in nectar synthesis and secretion. One of these genes was cell wall invertase 4 (cwinv4). The authors generated two Arabidopsis cwinv4 mutant lines to study gene function and used GoTaq® Green Master Mix to confirm the mutant genotype. Expression patterns of cwinv4 in wildtype Arabidopsis and an ortholog from Brassica rapa were examined in various tissues by RT-PCR. The reverse transcription step was performed using the Reverse Transcription System and 0.1µg of RNA. (4093)
Mobberley, J., Authement, R.N., Segall, A.M., Edwards, R.A., Slepecky, R.A. and Paul, J.H.
Notes: Certain bacteriophages can promote host cell sporulation under unfavorable conditions to increase survival of the host and prophage. These types of phages, known as spore-converting phages, have been found in terrestrial Bacillus species. In this article the authors examined the effect of prophages on sporulation of 11 Bacillus isolates from the Gulf of Mexico. Potential prophages in the Bacillus isolates were detected by PCR using unique PCR primer sets for each prophage genome and GoTaq® Green Master Mix. One of these isolates, B14905, was examined in more detail; the genome of this isolate was isolated using the Wizard® Genomic DNA Purification Kit, then sequenced. (4091)
Scafaro, A.P., Haynes, P.A. and Atwell, B.J.
Notes: The authors compared seedling growth rates, photosynthesis rates and expression levels of heat-responsive genes in the heat-resistant wild rice Oryza meridionalis and the domesticated rice O. sativa when grown at optimal and elevated temperatures. Proteins that were up- or downregulated in response to heat were identified by two-dimensional gel electrophoresis coupled with nano liquid chromatography on line with tandem mass spectrometry (nanoLC-MS/MS). Trypsin was used to cleave proteins prior to nanoLC-MS/MS. Semi-quantitative RT-PCR was performed using the GoTaq® Green Master Mix to determine if the heat-responsive proteins were transcriptionally regulated. (4092)
Trypsin Gold, Mass Spectrometry Grade
Covshoff, S., Majeran, W., Liu, P., Kolkman, J.M., van Wijk, K.J. and Brutnell, T.P.
Notes: The authors identified the maize homolog of hcf136 (Zmhcf136), a gene involved in photosynthesis, and used an RNA blot to determine if ZmHcf136 transcripts accumulate preferentially in mesophyll cells. DNA probes for Zmhcf136 and several cell-specific markers were generated by PCR using GoTaq® Green Master Mix, gel purified and radiolabeled prior to use in the RNA blots. To examine differences in protein accumulation and localization in wildtype and hcf136 mutants, proteins from subcellular fractions were subjected to two-dimensional gel electrophoresis, and spots of interest were excised, digested with Sequencing Grade Modified Trypsin, then analyzed by electrospray ionization-tandem mass spectrometry. (3883)
Sequencing Grade Modified Trypsin
Staniszewska, I., Sariyer, I.K., Lecht, S., Brown, M.C., Walsh, E.M., Tuszynski, G.P., Safak, M., Lazarovici, P. and Marcinkiewicz, C.
Notes: The authors investigated the ability of α9β1 integrin to act as a neurotrophin receptor and affect cell signaling pathways. As part of the study, RT-PCR was used to detect the presence of other neurotrophin receptors in their model cell line, SW480. Reverse transcription was performed using the Reverse Transcription System and 1µg of total RNA isolated using the SV Total RNA Isolation System. The resulting cDNA (5µg) was amplified for 35 cycles (β-actin as a control) or 40 cycles (TrkA and p75NTR) using GoTaq® Green Master Mix. RT-PCR results were confirmed by Western blot analysis. (3884)
SV Total RNA Isolation System
Kommagani, R., Payal, V. and Kadakia, M.P.
Notes: The authors examined transcriptional regulation of the vitamin D receptor (VDR) by p53 and p63, a member of the p53 family, under stressed and unstressed conditions. Reporter constructs with the full-length and minimal VDR promoters controlling expression of firefly luciferase were cotransfected with p53 or p63 expression constructs, and transcriptional activation of the VDR promoter was monitored using the Dual-Luciferase® Reporter 100 Assay System. Results were normalized to Renilla luciferase activity. Interaction between p73, another member of the p53 family, and the VDR promoter was examined using chromatin immunoprecipitation. The imunnopreciptated chromatin was reverse crosslinked, DNA was eluted and VDR and p21 sequences were detected by PCR using GoTaq® Green Master Mix. (3715)
Dual-Luciferase® Reporter 1000 Assay System
Yu, Q., Collavo, A., Zheng, M.Q., Owen, M., Sattin, M. and Powles, S.B.
Notes: The authors characterized mutations in the acetyl-coenzyme A carboxylase (ACCase) gene that confer resistance to the herbicide clethodim in the grass weed Lolium rigidum. The ACCase gene was amplified from clethidem-resistant and susceptible plants, then sequenced to identify previously unknown mutations. Amplifications of ACCase were performed using 300ng of genomic DNA and GoTaq® Green Master Mix. The Wizard® SV Gel and PCR Clean-Up System was used to purify PCR products directly or from agarose gels. (3721)
Wizard® SV Gel and PCR Clean-Up System
Martin, A.R., Dunstan, R.H., Roberts, T.K., and Brown, G.K.
Notes: GoTaq® DNA Polymerase was used in PCR to test dog blood for the presence of Babesia canis. Genomic DNA isolated from dog blood was analyzed with primers to the variable 5' region of the Babesia canis 18S rRNA gene. PCR was performed in 50µl reactions containing 1.25 units of GoTaq® DNA Polymerase and 10µl of GoTaq® Reaction Buffer. (3367)
GoTaq® DNA Polymerase
Hardstone, M.C., Baker, S.A., Gao, J., Ewer, J., and Scott, J.G.
Notes: Researchers used the GoTaq® DNA Polymerase in PCR screens for excisions around a CYP6d4 gene in the HA-1829 strain of Drosophila. PCR was performed in a 20μl reaction volume using 0.4 Units of GoTaq® DNA Polymerase, 2.75mM MgCl2 and 1μl of DNA (equivalent to the DNA in approximately 1/5 to 1/10 of a fly). (3363)
Dupont, K., Vogensen, F.K., and Josephsen, J.
Notes: GoTaq® DNA Polymerase was used in PCR to detect Lactococcus lactis phage DNA strains in whey samples. Phage DNA templates were amplified directly from DNase treated and boiled whey samples. For these reactions, the researchers use 0.25µl (1.25 units) of GoTaq® DNA Polymerase for each 50μl reaction. Primers were designed to distinguish various strains of Lactococcus lactis phage receptor-binding protein genes. (3362)
Theodoropoulos, G., Gazouli, M., Ikonomopoulos, J.A., Kantzoura, V., and Kominakis, A.
Notes: Researchers used GoTaq® DNA Polymerase to test sheep and goat blood samples for the presence of Babesia DNA. Primers were designed around the 18S rRNA sequence of Babesia sp. PCR was performed in a 50µl reaction volume using 1 unit of GoTaq® DNA Polymerase. Ten microliters of each amplification reaction were loaded on gels and subjected to electrophoresis. (3380)
Romero, H.M., Pell, E.J. and Tien, M.
Notes: PMSRA expression was examined in 4-week old plants exposed to 10μM methyl viologen, 100μM cercosporin, photo-oxidative stress or ozone. Samples were ground in liquid nitrogen and total RNA isolated. Total RNA (2.5μg) was reverse transcribed into cDNA with random primers d(N)10, then amplified using gene-specific primers for PMSRA1—PMSRA5 and an antibody-mediated hot start containing a mixture of GoTaq® DNA Polymerase and Taq antibody (BD Biosciences, Mountain View CA). In a total volume of 25μl, the RT-PCR reaction mixture contained 2.5 units of GoTaq® DNA Polymerase, 10mM Tris–HCl (pH 8.5), 60mM KCl, 2.4mM MgCl2 and 300μM of each dNTP. For each RT-PCR, a plant 18S universal internal standard (Ambion, Austin TX) was included as a loading control. Amplification reaction conditions were as follows: 27 cycles at 94°C for 45 seconds, 55°C for 45 seconds and 72°C for 1 minute. For each analysis, three rounds of RT-PCR were conducted with three independently isolated total RNA samples. Twenty microliters from each PCR were fractionated by 1.5% (w/v) agarose gel in Tris–borate EDTA buffer and stained with 0.5% (w/v) ethidium bromide. (3370)
Demars, J., Riquet, J., Feve, K., Gautier, M., Morisson, M., Demeure, O., Renard, C., Chardon, P., and Milan, D.
Notes: Fifteen microliter amplification reactions performed using 0.5 Units of GoTaq® DNA Polymerase and 25 ng BAC or genomic DNA were used as templates for sequencing portions of the QTL region of porcine chromosome 7. Amplification products were verified by gel electrophoresis followed by ethidium bromide staining. (3361)
Song, J.H., Bellail, A., Tse, M.C.L., Yong, V.W. and Hao, C.
Notes: Total RNA was extracted from human astrocytes and control A549 cells. First strand cDNA was synthesized from 3μg of total RNA using random hexamers. PCR was performed on the cDNA samples using primers for DR4, DR5, and GAPDH with GoTaq® Green Master Mix. The PCR was performed with an initial denaturation at 94°C for 2 minutes, followed by cycles of 30 seconds at 95°C, 30 seconds at 55°C, and 45 seconds at 72°C. The PCR products were analyzed on a 1.5% agarose gel and stained with ethidium bromide. (3372)
Owegi, M.A., Pappas, D.L., Finch, M.W. Jr., Bilbo, S.A., Resendiz, C.A., Jacquemin, L.J., Warrier, A., Trombley, J.D., McCulloch, K.M., Margalef, K.L., Mertz, M.J., Storms, J.M., Damin, C.A. and Parra, K.J.
Notes: The authors generated truncations of subunit d of a vacuolar proton-translocating ATPase pump to examine the protein's function. The N- and C-termini were required for subunit stability as well as pump function and assembly. Function was restored to the C-terminal deletions of subunit d by creating a chimeric protein with the C-terminus of a subunit d homolog from Thermus thermophilus. Three tandem repeats of a hemagglutinin (HA3) epitope were amplified and inserted at the N-terminus so that the chimeric protein could be visualized by Western blot with an anti-HA antibody. Initial amplification of the HA3 epitope and amplifications to confirm the orientation of the epitope were performed using GoTaq®Green Master Mix. (3720)
Denyer, M.S., Wileman, T.E., Stirling, C.M.A., Zuber, B., and Takamatsu, H.
Notes: In this study, GoTaq® DNA Polymerase was used in two-step RT-PCR. The ImProm-II™ Reverse Transcription System was first used to produce cDNA using an oligo d(T)15 primer. PCR was then performed using GoTaq® DNA Polymerase. Each reaction contained 2μl cDNA, 10μl GoTaq® Reaction Buffer, 1μl dNTP (10mM), 0.2μl GoTaq® DNA Polymerase, 1μl each primer (10pmol) and 34.8μl nuclease-free water. PCR was performed at 94°C for 30 seconds, 55°C for 30 seconds, 72°C for 60 seconds for 35 cycles, and 72°C for 10 minutes.PCR products were visualized by agarose gel electrophoresis containing ethidium bromide and then sequenced. (3368)
ImProm-II™ Reverse Transcriptase
ImProm-II™ Reverse Transcription System
Takami, M., Cho, E.S., Lee, S.Y., Kamijo, R. and Yim, M.
Notes: Mouse bone marrow cells and calvarial osteoblasts were cocultured for 6 days with or without 50 μM of IBMX. Total RNA was then isolated from the cells and cDNA templates prepared. cDNAs were subjected to PCR amplification with GoTaq® DNA Polymerase. Primers for mouse PDE4s, TRANCE, CTR, cathepsin K and GAPDH genes were used in this study. The PCR program was as follows: 32 (all mouse PDE4s, TRANCE, CTR, and cathepsin K) or 28 (GAPDH) cycles, after an initial denaturation step at 94°C for 3 minutes, then denaturation at 94°C for 30 seconds, annealing at 58°C for 45 seconds, and extension at 72°C for 60 seconds, with a final extension at 72°C for 10 minutes. (3356)
Jiang, G. and Sancar, A.
Notes: In this study, GoTaq® DNA Polymerase was used in amplification reactions to test for the recruitment of DNA damage checkpoint proteins RPA, Rad9, and ATR in ChIP assays. DNA was isolated from cells treated with UV irradiation or acetoxyacetylaminofluorene. The DNA and DNA damage checkpoint proteins RPA, Rad9, and ATR were crosslinked together and the complexes immunoprecipitated with antibodies toward RPA, Rad9, and ATR. Amplification reactions contained primers designed to amplify regions of the p53 and β-globin genes. (3366)
Mochizuki, K. and Gorovsky, M.A.
Notes: GoTaq® DNA Polymerase was used in RT-PCR. First-strand cDNA was synthesized from 3μg of total RNA. PCR was performed using the first-strand cDNA as a template, the primers TM4SF2, and GoTaq® DNA Polymerase, with 42 cycles of 94°C for 30 seconds, 50°C for 30 seconds, and 72°C for 60 seconds. (3354)
Weinberg, J.B., Stempflea, G.S., Wilkinsonb, J.E., Youngerc, J.G., and Spindler, K.R.
Notes: Mouse adenovirus type 1 (MAV-1) was detected in DNA extracted from the lungs of mice after PCR amplification of the E1A region of MAV-1. For these assays, 80ng of total DNA was added to a 20µl PCR reaction containing 0.5 units of GoTaq® DNA Polymerase, 4µl of 5X GoTaq® Buffer, dNTPs and primers for MAV-1 E1A. The amplified products were separated on a 1.8% agarose gel and stained with ethidium bromide. (3381)
Auge-Gouillou, C., Brillet, B., Hamelin, M.H., and Bigot, Y.
Notes: A transposase protein (Tnp) open reading frame (amino acids 1 to 345) was amplified from the Drosophila mauritiana Mos1 mariner transposable element using GoTaq® DNA polymerase. The Tnp open reading frame was then cloned with into the pGEM®-T Easy Vector and sequenced before further subloning. Tnp protein was purified and used in gel-shift assays to demonstrate Tnp binding regions in the Mos1 transposable element. (3344)
pGEM®-T Easy Vector System I
pGEM®-T Easy Vector System II


text:
Midwest Physicians, which is part of HCA Midwest Health System, is a network of experienced, multi-specialty physicians located throughout the Greater Kansas City metropolitan area
As a recognized leader in healthcare, HCA Midwest attracts physicians who are leaders in their field. In fact, our doctors and nurses are often ranked among the best in the region, trained at some of the nation's leading medical and nursing schools.
A primary care provider (PCP) is a health care practitioner who sees people that have common medical problems. Your PCP is often involved in your care for a long time, so it is important to select someone with whom you will work well.
Specialists are trained in a specific area of medicine that focuses on a narrow range of services, procedures, body systems or patients.
Review our calendar for health screenings and more, and utilize our health encyclopedia to research medical conditions and treatments.
We offer a number of career opportunities for individuals who are committed to compassionate medical care.


text:
Movement 1: Allegro con brio
Movement 2: Andante cantabile, con variazioni
Movement 3: Menuetto: Quasi allegro
Movement 4: Prestissimo
Within weeks of his arrival in the imperial capital Beethoven became a house guest of his principal patron Prince Karl Lichnowsky, a talented amateur pianist who kept his own string quartet. The Prince held regular private soirées at which Beethoven would astonish the company with his brilliant keyboard improvisations; and it was at one of these occasions that he chose to introduce his first important Viennese compositions, the set of three piano trios which he published by subscription in August 1795 with a dedication to Lichnowsky. His erstwhile teacher Haydn, recently returned from his second triumphant London visit, was present, and warmly praised the E flat and G major trios. But he suggested that the third trio, in C minor, would not be easily understood by the Viennese publica well-intentioned remark that the ever-touchy Beethoven put down to envy.
Beethoven was determined to impress and challenge the Viennese musical elite with his first published opus. And with their weighty four-movement structures and urgency of musical dialectic, the Op 1 trios must have seemed like a full-frontal assault on the traditional notion of the piano trio: what had been an intimate domestic medium in Mozarts and Haydns hands suddenly became a symphony for three instruments. In the first two trios Beethovens subversiveness was still cloaked in the language of the classical comedy of manners. But in the C minor, No 3, it erupted in a work of startling explosive vehemence and dark lyric beauty.
While it may have shocked some of its early listeners, Haydn included, the C minor Trio gradually became one of Beethovens most popular chamber works. More than two decades later, in 1817, an amateur composer named Herr Kaufmann submitted to him an arrangement he had made for string quintet, with two violas. Beethoven had it copied out, decided he could do better, and made wholesale improvements to the part-writing. At the top of the score he wrote a jokey, ironic preface, mocking Kaufmanns efforts as a three-voice quintet which he had raised from the greatest wretchedness to some respectability, sacrificing the original as a solemn burnt offering to the gods of the underworld.
Beethoven did not jettison Kaufmanns ideas altogether; and the final arrangement, published in 1819 as Op 104, was thus something of a composite effort. The first violin line occasionally betrays its keyboard origins. Yet for the most part the quintet arrangement sounds thoroughly idiomatic, with the most obviously pianistic passages recast for strings and the textures enriched by many touches of imitation. Beethovens only, minor, structural change was in the finale, where the recapitulation was originally introduced by a flamboyant chromatic scale for the keyboarda kind of mini-cadenza. For the quintet arrangement this lead-back becomes thematic rather than decorative: the lower parts sound the first four notes of the main theme, while the chromatic scale, now in the first violin, is reduced from eight bars to four.
The mysterious, pregnant unison opening (deprived of its initial turn in the quintet version) is, coincidentally or not, reminiscent of Mozarts Piano Concerto in the same key, K491, a work Beethoven is said to have greatly admired. But the music is profoundly Beethovenian in its abrupt, extreme contrasts, with violent rhetoric (the first page alone is peppered with sforzando accents) alternating with intense pathos and yearning lyricism. The famous heroic narratives of Beethovens middle period are already in view. There is a thrilling moment at the start of the development where the original pianissimo echo of the main theme a step higher now moves a semitone lower, spiriting the music to a strange new tonal region (C flat major, enharmonically spelt as B major). It is typical of Beethoven, at any period, that the quiet opening phrase returns as a strenuous fortissimo at the start of the recapitulation. Then, in perhaps the most breathtaking stroke of all, the music slips first into C major, pianissimo, with the cello taking the lead (imitated in the quintet arrangement by the first violin), and then into the Neapolitan key of D flat for a new cantabile development of the main theme.
After this high-pressure sonata drama, the slow movement, a set of variations in E flat on one of Beethovens characteristically plain, hymn-like themes, lowers the tension. But there are plenty of inventive, authentically Beethovenian moments: the boisterous third variation, with its brusque sforzando accents and twanging pizzicatos; the fourth, in E flat minor, initiated by a plangent cello solo; or the coda, beginning with a rich chromatic reworking of the theme.
The third movement, somewhere between a minuet and a scherzo, returns to the agitated, explosive C minor world of the first movement. In the C major trio section Beethoven has fun roughing up the cellos lilting Ländler melodyenriched in the quintet version by a countermelody for second violawith cussed offbeat accents. The Prestissimo finale (the tempo marking is typical of the young Beethovens determination to be extreme) juxtaposes violence, suppressed agitation and, in the E flat second theme, lyrical tenderness. In the recapitulation the second theme turns from C major to C minor, with deeply pathetic effect. The astonishing coda, held down to pianissimo for most of its eighty-seven bars, slips mysteriously to B minor and then moves, via C minor and F minor, to C major. But the ending is uneasy and equivocal, with minimal sense of resolution (the recent memory of F minor is too strong for that), let alone of major-keyed optimism.
from notes by Richard Wigmore © 2009


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Give nature photos, family pics or place cards a seasonal lift. Drill a small hole in the top of a walnut. (Pick nuts with flatter bottoms so they stand up easily.) Cut a 6-inch piece of 18-gauge wire. Wrap one end of the wire twice around a wide marker. Remove the marker, and pinch the loops together. Insert the other end into the nut. Slip a photo or card between the loops.
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This is a fantastic hardback book from East of India that would make a great gift for anyone planning to keep a record of their future or past travels. The book has a real retro aged feel to it with a washed out dated look. The cover is partly a very pale blue colour with white polka dots and partly an image of an aged UK map and is decorated with 'My Travel Book', and an old travel ticket. Inside the pages are lined on one side for writing your memoires while the other side has deep pockets for saving all your momentos.
Our East of India collection available to buy here at The Gifted Penguin consists of beautiful handmade products which will add a rustic but modern feel to your home or make the perfect gift for a loved one. East of India creates products with special finishing touches and as their items are handmade they really care about the end result and the quality. All of the East of India products and gifts are made from the finest materials. They love creating unique gifts which can proudly sit in any room in the home and are sure to gather interest from all of your family and friends. No matter what the occasion, whether it is a special birthday, baby's christening or your best friends' wedding, you will find a perfect gift from the East of India range. Whoever you are buying for will love to receive a gift from this beautiful range. It is always important to find that ideal present for somebody that they can keep for many years to come. You are sure to accomplish this with a great gift from this range. From wooden signs to photo frames and from keepsake boxes to boxes for you to keep recipes or sewing kits in, there are so many specialized items for you to choose from which will all fit a purpose for someone you love. East of India has many beautiful boxes and keepsakes for you to keep christening memories in. If your friend's baby boy or girl is being christened or perhaps a family member, they will love these pretty boxes and having a special piece from everybody to put inside and keep for when their child has grown up will be lovely. It is a great way to make sure nothing goes missing and that memories are kept secure.
If you like the East of India products we have in our collection, make sure you take a look at our gift ideas for her that are available. Also take a look at our great page for gift ideas for him, this will help you find a special something to put inside the keepsake box of your choice.


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SouthPeplum Dolly Coat
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Tuesday, March 27, 2012
A Perfectly Good CatLoaf
23 comments:
- Simba said...
It is hard work, Spitty. But you are good for it.
- March 27, 2012 at 3:59 AM
- The Furries of Whisppy said...
Hahaha. You sure showed her with just a *flick*. MOL.
- March 27, 2012 at 4:18 AM
- Katie Isabella said...
Spitty..no worries. The Admiral heard and said no worries. xoxoxoxox
I haven't been in a catloaf YET! Matter of fact, while dictating this I'm doing it with shouting type meows as I fly by on thundering feets!
xoxox - March 27, 2012 at 4:27 AM
- Fuzzy Tales said...
Well done! Honestly, we're so sick of the flashy box. Derry has taken to running away whenever he sees our human even just pick it up!
- March 27, 2012 at 6:28 AM
- Brian said...
Ya done good King Spitty!
- March 27, 2012 at 6:37 AM
- William said...
You have to keep them frustrated just enough, Spitty!
- March 27, 2012 at 6:41 AM
- Pandafur said...
Eksellent tail move! Thanks you fur teh kind werds on mai bloggy, I duz appreyshiates it lots.
- March 27, 2012 at 7:19 AM
- Cathy Keisha said...
Good for you Spitty. I enjoyed your visit last night immensely. Hope you liked the Silvervine P-low. Sorry I hissed at you at the beginning when you first touched it. Hope I made up for it later.
We've got to talk about the fairy dust you have sprinkled on the blog. It ain't manly and it ain't kingly. It's just plain freaky. It freaks me out to have that dust follow my fursor around. - March 27, 2012 at 7:30 AM
- Shaggy, Scooby and Scout said...
And you do it so well, Spitty.
- March 27, 2012 at 7:42 AM
- Mark's Mews (Ayla, Iza, and Marley) said...
Not ta be annoyin, but iffen we keep seein fairy dust drippin from our mousie evry time it moves, we are gonna BITE someone!!!
- March 27, 2012 at 8:26 AM
- Katnip Lounge said...
Your tailio is simply a Loaf Condiment, like ketchup, only tastier.
- March 27, 2012 at 9:04 AM
- The Cat From Hell said...
Dear Spitty
Hmmm, if yous is a cat loaf, if me was loafing beside yous, would me be mashed potatoes?
Just wondering
Nellie - March 27, 2012 at 10:53 AM
- Tucker The Crestie said...
Good thinking, Spitty. Never let them take you for granted!
- March 27, 2012 at 11:03 AM
- Sparkle the Designer Cat said...
I have photo ruining down to a fine art, Spitty - I look right in the camera while my human is messing with the settings - and look away when she is ready to shoot!
- March 27, 2012 at 11:56 AM
- Christine and FAZ said...
You still look good through. FAZ
- March 27, 2012 at 12:40 PM
- ABBY said...
You are royally gorgeous Spitty!
♥Abby♥Boo♥Ping♥Jinx♥Grace♥ - March 27, 2012 at 3:35 PM
- Callie (a.k.a. Trouble) said...
Spitty, you need a throne to sit upon and view your audience.
- March 27, 2012 at 4:35 PM
- The Island Cats said...
Spitty, you really do hafta do something about her. She is not obeying your commands!!
- March 27, 2012 at 4:46 PM
- Andrea and the Celestial Kitties said...
Great job! We love catload with a handle!
Star was glad you were at Leo's party so she had someone to snuggle UTB. She's still purr-ey today! - March 27, 2012 at 4:48 PM
- Katie Isabella said...
Frankly my dear Sire...I LOVE your sparkles. xoxoxoxo
- March 27, 2012 at 7:11 PM
- Luxington said...
Good move Spitty, good move. I totally understand.
- March 27, 2012 at 9:20 PM
- Clooney said...
Spitty, you are quite the clever guy (of course we have all known that forever but just sayin').
- March 28, 2012 at 12:02 PM
- Mark's Mews (Ayla, Iza, and Marley) said...
Sorry, we keep tryin ta whap the sparklies, and that makes us step on the keyboard, and THAT causes trubbles. We'll just hafta learn ta restrain our whappin instincts...
- March 28, 2012 at 1:06 PM


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Joel Feick is an anchor on NBC25 Monday through Friday from 5am - 7am.
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VT-12TEDTS-2Z - Vinotemp 12 Bottle Dual Zone Wine Cooler - Black
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im not liking the demonic shield....it doesnt even fit the hero....i really dont get where it fits in
It really looks like the blade is actually blocking the barrel of the gun, which bugs me. He also smacks of a LoL hero.
Ok... So more people voted it "down" than people actually watched the spotlight...
I personally really liked the spotlight, the music was really good and it was very informative. The only thing it missed was numbers but no spotlight has numbers.
Community Contributions Fan
I feel like this hero is just a ranged nomad.
not skillwise
but item wise.
Ghost marchers, helm, shroud, shieldbreaker, bulwark.
but then again when I think about it. A lot of heroes can make these items and instapwn someone. I just see it coming that gunblade will be a oneshot dude just like nomad. none the less, gonna buy him.
As many have stated this hero seems uninspired with abilities that come across as recycled from other heroes. IMO S2 has a great streak of qualities heroes, I am inclined to believe this is an abbreviation opposed to the beginning of a bad trend. Regardless, hopefully sales reflect the quality and this week S2 see's the difference from a great original concept (Kinesis) to a pretty poor one (Gunblade).
As for the spotlight videos, this is defiantly another low point. Spotlight videos/the horsemen videos seem to have lost a bit of steam and creativity lately. Again, I am willing to give it a month or two and see if S2 returns to form before I become concerned.
The important thing is for the consumer to express our opinions and hopefully S2 responds with stronger quality control.
Last edited by Trolloc; 03-14-2012 at 02:06 PM.
What is with that baby taunt segment? That looks like something I'd have done when I was 10, and then deleted it out of shame 3 years later.
looks like Gunblade is going to have a Warhammer 40k spacemarine themed EA skin...
This spotlight was poorly made, pathetic to say the least.
Good thing about playing with friends: you will never get kicked when picking Night Hound as Orange or Brown.
I'll post it again and again.
Smyger was on break this time, so Viole had to work with some random volunteers who just did this for the first time, that in turn encountered their own problems.
Thus the quality of this Spotlight isn't of the usual epic one.
Please note that Smyger and RuthlessVoid took a break this spotlight for personal reasons. I had to do with a make-shift crew of people I've never worked with. We tried some new things and added a bit more flair to the scenes. Let me know how you all liked it, what suggestions you have, what you think could be improved, and anything else!
Also, let Cami and Murray know how well they did in such a tight squeeze
Hero named Gunblade. Only uses guns. The gods of design look favorably upon this company.
This hero looks terrible.
I'm sorry, but I see absolutely no redeeming features in his design.
This is the kind of hero design you should be scrapping, S2.
My hero suggestions:
Gust - Druidess - Mobeus - Stone Angel
this hero could either be very OP or not good at all, terrible design.


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Going from darkness to light
By GARY ANDREWS
Editor and Publisher
For several months we watched and heard about the miners in Chile being trapped 2,000 feet below the earth's surface. For 17 days no one knew if these men were dead or alive but through the effort and energies of the company, families, and state officials contact was eventually made with the miners.
It was a tremendous day when the voices were heard from below and how these men congregated together to lean on one another for support. Even after the 17 days of not knowing, it would be another two months before extraction of these miners would come to fruition.
These miners had been well versed in what to do in case of an emergency and the company was properly prepared with life's necessities should an emergency such as this one happen.
There were many medical concerns about these men as they would come from being under ground with only artificial light into the brightness of the sunlight. The evacuation started at night and continued for 24 hours until each miner was rescued and back above ground.
No medical emergencies existed other than what problems might have been coming from the darkness to the light. To help combat this each miner was given sunglasses to hide their eyes until they eventually could face the light again.
This is a story that ended well, however there are many of our friends and neighbors that walk in darkness everyday and don't even know it. Just as these miners were trapped and couldn't see so are many people that are caught in satan's arms and resist the light of Jesus.
We don't have to be 2,000 feet below the earth to see total darkness. We may be involved in situations that we don't need to be in, however we have allowed ourselves to be brought in through sinful actions and desires.
Just as the miners were brought back to light there is hope for us that have fallen short of our Christian morals and values being brought back to the light. Sunglasses were used for them and the Word of God is needed for us. Both of these will supply us the needs of facing the light and getting away from the blinders that has held us captive for so long.
Remember what Jesus told Saul on the road to Damascus. Acts 26:17-18 states, "I will rescue you from you own people and from the Gentiles. I am sending you to them to open their eyes and turn them from darkness to light and from the power of Satan to God, so they may receive forgiveness of sins and a place among those who are sanctified by faith in me."
Prayer: Lord use me to help those in darkness to see your light. Guide me in my actions and thoughts. Amen.
(Suggested daily Bible readings: Sunday - Acts 13:8-11; Monday - Matthew 8:5-12; Tuesday - Isaiah 50:10-11; Wednesday - Psalm 139:11-12; Thursday - 1 John 1:5-10; Friday - 2 Chronicles 15:1-7; Saturday -1 Corinthians 4:1-5.)


text:
In a sometimes contentious cross-examination filled with testy exchanges in the George Zimmerman trial, defense attorney Don West peppered the prosecution's star witness, Rachel Jeantel, with questions Thursday about the consistency of her statements related to the night Trayvon Martin died.
At one point, West even asked Jeantel if she understood English.
"I don't understand you, I do understand English," Jeantel replied.
"When someone speaks to you in English, do you believe you have any difficulty understanding it because it wasn't your first language?" asked West.
"I understand English really well," said Jeantel.
The 19-year-old, who had been asked several times during her testimony to speak louder or more clearly, said Creole is her first language.
Phone records show that Jeantel was talking to Martin moments before he was shot to death by Zimmerman last year.
Zimmerman, a former neighborhood watch captain, is charged with second-degree murder in the death of 17-year-old Martin in Sanford, Florida, on Feb. 26, 2012. Zimmerman told police he was pursuing the teenager because there had been a rash of crime in the area. A confrontation ensued, and Zimmerman said he was forced to kill Martin in self-defense.
On Thursday, her second day on the stand, Jeantel offered terse replies when asked by the defense about her claims that Martin told her the night he died that someone was following him and that a fight followed.
"Trayvon got hit," said Jeantel.
"You don't know that, do you?" West shot back.
"No, sir," said Jeantel.
"You don't know if Trayvon got hit, do you?" asked West.
"No, sir," said Jeantel.
"You don't know that Trayvon didn't, at that moment, take his fist and drive it into George Zimmerman's face, do you?"
"No, sir," said Jeantel.
West also challenged Jeantel several times over whether Zimmerman approached Martin the night of the shooting, but she maintained her story.
"If he was going to confront the man he would have told me, 'I am about to confront the man and see what he wants.' He did not tell me that, sir. He just told me he tried to get home, sir. But the man was still following him, sir," said Jeantel.
Jurors also heard the 911 call made by neighbor Jenna Lauer that captured the sounds of yelling and the gunshot that killed Martin.
Lauer testified that she muted her TV after hearing voices take part in what she describes as a "three-part exchange." She couldn't identify what exactly was being said.
After hearing the voices, Lauer said she heard another sound.
"I just heard whoever was out there scuffling around... it sounded like sneakers on pavement and grass," said Lauer. "Like someone was running on the pavement and on the grass -- it sounded like that."
The scuffling turned to yelps, which then turned into cries for "help," according to Lauer.
"It sounded like they were in our living room -- it was right there," said Lauer. "It just sounded like they were desperate and whoever it was really needed help or wanted help."
Another neighbor, Selma Mora, said she walked out her back door after hearing the gunshot, which she at first thought was a skateboard smacking the pavement.


text:
The United States has been at war for four years. Just about everyone agrees that strategic mistakes were made, and nobody is blaming the troops. I get frustrated as I listen to most of the arguments about the war. So many times it boils down to name calling and over-simplification. Meanwhile, the world becomes a more dangerous place. Lets talk about what America should do now. Please limit comments to your position rather than attacking other views.
Patrick Baz/AFP - Getty Images
US soldiers scan rooftops looking for a sniper in Baghdad's Haifa street on March 19. Congress is expected this week to consider budget legislation for the Iraq war that includes a provision requiring a US withdrawal by September 2008.


text:
Paris Paris Center Hotels + 2 Star + Artcurial + Restaurant
Know When You're Going?
More Search OptionsStar Rating
- 2 Stars
Hotel Amenities
- Restaurant
0.53 mi/0.86 km from search point (short walk)
"I spent five days here at the end of November, it is a comfortable modest hotel, very... Read more"
"Hotel had good location near metro stop ecole militaire . We were on 4th floor elevat... Read more"
Note: The nightly rates shown above do not yet include Tax Recovery Charge & Service Fees or (if applicable) Extra Person Fees, Child Fees, Additional Mandatory Hotel Charges or optional incidentals, such as parking, room service or movies.


text: Sylvia woke up early the next morning. Shevlooked over the three others around her. Ocelot seemed to be doing much better now, he would just need rest. Dietrich, well, she couldn't tell, it seemed like the bleeding had stopped, but she had never encountered a creature like her, so despite some medical training she had picked up, it wa impossible to tell. She decided Dietrich was ok since Lyon was still curled up, and intact. She sat next to him and gently began petting him. She didn't want to wake him, but she was growing a bit attached to him.


text:
The Detail.MGX Pendant Light
Filed under: Decor
Now here's a lamp that truly can't be copied or created by any other artist -- it's literally got designer Dan Yeffet's fingerprints all over it. In an endeavor to create something that represented privacy (or a lack of) he took his own fingerprint and stretched it over a sphere shape, resulting in a truly unique pattern of light shining out from between the channels.
At first I wasn't that crazy about this design because it looked like zebra stripes and I'm not one for animal prints. But once I realized it's fingerprints I like it a lot more! Funny how that works. The Detail.MGX pendant light measures 24cm wide (the sphere) and is available from Within 4 Walls for £1948.


text:
For years now, there have been rumblings about the eBox, or its other moniker, the iSec. Yet, the console was pushed back, and then it seemed to vanish completely. Well, it's back and slated to come out soon. Real soon.
Now called CT510, this is the Chinese game console the Lenovo backed and co-invested in—the first of its kind. In China, video game consoles are banned. Yet, Beijing Eedoo Technology Ltd., the company developing the CT510, said this week that the console will be released in China on April 29. It will be priced at 3,799 yuan ($600) per unit, and it comes with eight pre-installed games.
Last fall, it was priced at 3,000 yuan, which was then $470.
Like Kinect, the CT510 features control-free motion-controls. According to Eedoo head Jack Luo, the CT510 is only the second home console to feature an entirely controller-free home console. The console is targeting casual players and not hardcore gamers. It also seems to be targeting people with $600 to spare.
China Daily reported that the console was delayed for about a year because, as Luo said, Eedoo required more time to "further improve the product and provide a better user experience".
Even though the console is slated to come out, it's unclear how this machine can bypass the Chinese game console ban, which outlaws "game boxes", and be legally sold in China.
And once it does go on sale, does that mean that Japanese and American console companies can legally sell their hardware in China?
The PS3, the Xbox 360, and the Nintendo Wii are widely sold throughout China, but via the country's gray market.
Chinese game console about to hit market [China Daily]


text:
2012년10월24일, Hotels.com 고객 이용 후기
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고객이 별도로 요청한 사항은 체크인 시 가능한 경우에 한하여 제공되며 추가 요금이 부과될 수 있습니다. 또한 반드시 보장되지는 않습니다.
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이 숙박 시설은 별도로 인증된 제3차 기관 인증 프로그램인 Green Key Eco-Rating Program에 참여하고 있습니다. 제3차 기관 인증은 해당 숙박 시설이 환경, 커뮤니티, 문화 유산, 지역 경제 중 하나 이상의 분야에 어떠한 영향을 미치고 있는지 평가합니다. 이 숙박 시설의 친환경 관광 사업에 대한 객관적인 평가는 제3차 기관 인증 프로그램과 관련이 없는 독립된 평가자 또는 감사자에 의해 수행되었습니다.
숙박 시설 내에는 실내 수영장 외에 2 개의 스파 욕조가 마련되어 있습니다. 기타 레크리에이션 서비스/시설로는 24시간 피트니스 시설도 마련되어 있습니다.
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체크인 시 각종 서비스 이용 요금이 발생할 경우를 대비하여 정부에서 발급한 사진이 부착된 신분증과 신용카드 또는 현금으로 보증금이 필요합니다.
고객이 별도로 요청한 사항은 체크인 시 가능한 경우에 한하여 제공되며 추가 요금이 부과될 수 있습니다. 또한 반드시 보장되지는 않습니다.
Clean and in the center of everything.... Awesome stay kids a loved the water slide검증된 Hotels.com 숙박객 이용 후기
The people who worked at the front desk were very helpful with information about shopping and dining availability. The location of the hotel is very convenient for shoppers.검증된 Hotels.com 숙박객 이용 후기
"Short Visit"
The staff were very friendly and accommodating. Breakfast selection was very good. The rooms were very quiet. Excellent location. Would definitely stay here again.검증된 Hotels.com 숙박객 이용 후기
Friendly staff, clean room and pool. (pool chairs needed cleaning. Great breakfast included in the price.검증된 Hotels.com 숙박객 이용 후기
"Great service!"
The highlight of our stay at the Dartmouth Hampton was the quality of service. From the front desk staff to housekeeping, everyone was willing to go above and beyond to make sure our stay was comfortable. My mother has many food allergies and has to pack her own meals when she travels. The room she was staying in didn't have a fridge, but the staff […] offered to keep it in their fridge and brought it out for her whenever she needed it. The rooms were always very clean and the friendly housekeeping staff would leave little notes on the notepads. The hotel itself was clean and modern with a nice swimming pool. As far as free continental breakfasts go, this hotel has one of the best! The bacon, eggs and waffles were terrific! The location of this hotel is great if you want to travel outside of Halifax. It's close to the highway so you can get around quickly. The downfall is it's not close to any tourist attractions. The harbour front is about a 20 min drive and the airport is around 15 min. If you like shopping there is a great strip mall right next to the hotel and major shopping center nearby. There's also a handful of restaraunts and a movie theater within walking distance. 더 보기검증된 Hotels.com 숙박객 이용 후기
"Classy place for a great price."
Wonderful place to stay. First time there definetly will return.검증된 Hotels.com 숙박객 이용 후기
"Always satisfied"
Excellant as always and close location to all.검증된 Hotels.com 숙박객 이용 후기
The room was small and it did not have a fridge so I got one brought up cost me $20 extra dollars sad when your room does not come with a fridge not satisfied at all would not stay there again.검증된 Hotels.com 숙박객 이용 후기
"Great stay with great pool for kids"
We stay here with the kids from time to time when we are down in Halifax. Pool is great for the little ones. Breakfast is good as well검증된 Hotels.com 숙박객 이용 후기
Fine for shopping nearby.검증된 Hotels.com 숙박객 이용 후기
"Maritime Race Weekend"
The Hotel is very easy to get to and is very visible at night to tired highway travelers. The morning breakfast choices are numerous and plentiful. Helps you get your day started right. The room was very comfortable and clean. Having WIFI available for free is much appreciated. Being able to park for free is outstanding.검증된 Hotels.com 숙박객 이용 후기
"great place"
Wonderful stay as always. This was our 7th time at this spot!검증된 Hotels.com 숙박객 이용 후기
"Very convenient"
Enjoyed the comfort; surroundings and amenities of the hotel. Very quiet.검증된 Hotels.com 숙박객 이용 후기
We enjoyed are stay room was very clean staff was very friendly . breakfast was great. Would stay there again.검증된 Hotels.com 숙박객 이용 후기
"Back to school shopping"
I booked this hotel for a back to school shopping trip with my son. We have stayed at it a handful of times before......but it has been a couple of years. I was surprised at some of the disrepair that has presented itself since our last visit. The pool area specifically is quite a turn off........there is a "ring" around the pool from the water line […] and the slide area is stained very badly with with iron and hard water. I would think that some general maintenance and cleaning would resolve both of these issues. The pool deck is filthy with dust/dirt and hair. The pool and each of the hot tubs are full of sediment and dirt. Quite disappointing. In our room the floor had obviously not been vacuumed........my son found a pile of something (it was unidentifiable) under the edge of the bed while picking up one of his toys. For a hotel that cost that much I expect a lot more. 더 보기검증된 Hotels.com 숙박객 이용 후기
"nice hotel. many places to shop and eat close by."
Had a very pleasant stay. This hotel is very clean and quiet. Love the location.검증된 Expedia 이용 후기
"The comforter"
The bed was very comfortable but the comforter was not. There was only the top sheet and the comforter so you were either too hot or too cold. The comforter was very lumpy due to clumps of inner stuffing compiling in certain areas. I wish there had been some kind of blanket between the top sheet and the lumpy comforter. Otherwise, it was very pleasant […] staying at the Hampton Inn and suites. Breakfast was fabulous! Thank You 더 보기검증된 Expedia 이용 후기
"hotel was beautiful . Can't wait until our next st"
had a nice relaxing stay ..Room wS clean and despite the hotel being full ot was very quite검증된 Expedia 이용 후기
"Very Nice!"
Hot breakfast that was included was a nice change from the ho-hum continential breakfasts offered at most other places. Pool was clean and well maintained (salt - not chlorine). Close to major highway - easy to get to downtown Halifax using the toll bridge. Stayed only one night - our mistake! We should have stayed longer!검증된 Expedia 이용 후기
"Great Stay!"
We had a great stay. Very nice to have a complementary breakfast in morning.검증된 Expedia 이용 후기
"Excellent value for families (Dartmouth/Halifax)"
The price point is within reach of most families and the abundance of services included for the price makes this an excellent value. The pool is relatively small but has a great slide to entertain kids. The breakfast selection in the morning offers something for everyone. Free coffee and tea all day is an added bonus. The workout room, business room […] (with printer/fax), laundry ($2 per cycle), and on-site convenience store (milk, water, pop, juice, snacks) are all added bonuses. The hotel is within a complex that includes a movie theater, many different restaurants, and many different retail stores, all within walking distance. Walmart is also very close and it has food/drinks so you don't have to purchase them at the hotel lobby. The hotel is 5 minutes from Halifax and just off the highway. The staff was wonderful, all round, and exhibited a "can-do" attitude. An example of this occurred as I was checking in. I arrived to find I hadn't booked enough rooms, but the front-desk resolved my issue in minutes. This is an excellent value for families travelling to Dartmouth or Halifax. 더 보기검증된 Expedia 이용 후기
"Great location"
Front desk staff were very helpful making reservations for us, room was clean and everything worked fine. Was located in a great area with lots of shopping and restaurants. Would definitely stay here again.검증된 Expedia 이용 후기
"nice clean hotel close to everything"
Nice indoor pool for adults and kids with lot of space.검증된 Expedia 이용 후기
"ok stay, little overpriced"
It was nice that it was in close walking distance for Dartmouth Crossing but did not like that there was no mini fridge in the room and the shower was dreadful...no pressure at all! I am all for saving the planet but it took me 5 solid minutes to get my hair wet enough to shampoo and then twice that to get the shampoo out! Positive note, beds are very […] comfortable. 더 보기검증된 Expedia 이용 후기
"Shopping in Halifax."
The hotel itself was very nice . My stay was good, but , the bed. Was not that comfortable and the that were there were terrible. They were standard pillows for a king bed . Also no support what so ever. Our room was clean and everything in its place. The complimentary breakfast was o.k. But the scrambled eggs were like little sponges. So if they can […] improve on this it be a great place to stay. I feel you can not fix a problem unless you know about one. Hopefully this will help. Thanks again. 더 보기검증된 Expedia 이용 후기
"Pool was really not clean!!"
Our stay was ok and the staff was really nice....The pool and hot tub were not clean...even sand on the bottom of the hot tub...The pillows in the room were really not comfortable...검증된 Hotels.com 숙박객 이용 후기
Very nice hotel, great breakfast with your own waffle maker, great pool with slide for the kids, modern and comfortable rooms. All for an affordable price. A lot of shops and restaurants nearby. Continuous coffee and tea available in the lobby. Excellent검증된 Expedia 이용 후기
"Hampton Inn Review"
Clean room, friendly staff; what more could you ask for. Housekeeping even left a handwritten note saying to let them know if we needed anything (we were impressed).검증된 Expedia 이용 후기
"Close to all the shops and stores."
Really liked everything but the breakfast. Too many people. Had to wait for the toast. Things were a bit confusing. Love the location the rooms and the staff검증된 Expedia 이용 후기
"Beautifully decorated"
Very nice hotel, loved the rooms, the dining room and the breakfast was wonderful! My kids loved the pool and the slide!검증된 Expedia 이용 후기
"conveniently located"
Conveniently located for shopping and easy access to the highway. Stayed in a double queen suite and enjoyed the space. Great included breakfast.검증된 Expedia 이용 후기
"Nice room, rude front desk staff"
We had some issues with the water pressure but we were able to move to another room. The hot tub jets in the pool area did not work and the pool area could use some work. These were not big issues for my family and I. My only real complaint was the guy at the front desk when we checked in. I found him unpleasant and anything but welcoming. The rest […] of the staff were great. 더 보기검증된 Expedia 이용 후기
"Excellent hotel with plenty of amenities"
Waterslide is a nice addition to the standard pool, and judging my the number of kids, very popular. Breakfast was excellent검증된 Expedia 이용 후기
"Great for family with teenagers"
Room was great. We had a large room with 2 double beds and a sofa bed so great for teenage boys to each have their own bed. Very clean hotel and good breakfast buffet included with the room rate.검증된 Expedia 이용 후기
"Great waterslide for this family friendly hotel"
The kids rated the speed of the waterslide as #1 on our vacation where several other waterslides were involved. Great hote would recommend!검증된 Expedia 이용 후기
"Wonderful Service"
It was wonderful. The service was Great. Very Friendly staff. We would stay again.검증된 Expedia 이용 후기
I was very disappointed in the Hampton in Dartmouth! The pool area was not clean, especially around the water lied and the floor of the whirlpool was full of sand! The Suite was not a Suite at all...just a oom with a kitchenette...all open and no difference in I've than other rooms I'm sure! We will not be returning to any Hamptons or a suite for s […] ure!! 더 보기검증된 Hotels.com 숙박객 이용 후기
"Great Area"
Hotel was very clean with friendly staff. Great location to stay and shop.검증된 Hotels.com 숙박객 이용 후기
"Great Trip"
It was definfitly the cleanest and pleasant hotel i've ever stayed at and the workers were very kind. Very nice lay out, great sized rooms and bathrooms and location was perfect.검증된 Expedia 이용 후기
"Pet Hotel?"
Very convenient location, close to shops, restaurants and the highway. Down side is that although both the hotel's and Expedia's websites mention that this place is pet friendly, unless you're someone who reads the "fine print" you're not going to see this and be prepared to see a lot of dogs there like we did. Don't get me wrong, I like dogs as much […] as anybody else, but its quite a shock to see as many dogs as we did on our recent stay and they weren't confined to just one floor, they were on all floors. For a while we thought we had checked in to a local kennel instead of a hotel. So if there are that many dogs staying there, how many cats stay there too, and that's where I get fearful of booking there again due to my allergy to cats. No matter how much they say they give the rooms the once over, there's no way to fully eradicate pet hair from a room once it has had pets in it. 더 보기검증된 Expedia 이용 후기
"Nice hotel for the value"
Great hotel, large clean rooms. A nice pool with water slide. The staff were very friendly and courteous. The only down side was the breakfast buffett. While cold cereal and the waffle station were great, the eggs were too salty and not very tasty. There are plenty of meal options in the Dartmouth Crossing area if you are a bacon egg person.검증된 Hotels.com 숙박객 이용 후기
"view to all the stores, wich was nice!"
can't ask for more, it was superb!!!검증된 Hotels.com 숙박객 이용 후기
"great location"
This hotel is in a great location! Staff could have been a little more friendly and give us more details upon arriving like when check out time was, what time breakfast started or ended, about the pool or even how to find our room! (But of course this is just minor improvements that could be done!) Also big variety of breakfast foods but room for improvement […] there as well! ( on a taste scale 10 being the best it was a 6.) 더 보기검증된 Hotels.com 숙박객 이용 후기
"Thumbs up for a GREAT family vacation!!"
We really enjoyed our stay at the Hampton Inn & Suites at Dartmouth Crossing. The hotel was spotless and the staff was very friendly and helpful. We enjoyed a fantastic hot breakfast each morning and access to complimentary coffee/tea throughout the day.They even had "movie night" in one of the meeting rooms for the kids - what a great idea!! The kids […] enjoyed the pool and water slide the most. We all slept very well during our stay. Though the parking lot was full, we didn't hear a sound at night. Didn't hear stomping in the hall or doors slamming, as you normally hear at hotels. Also, being located right in Dartmouth Crossing Village Shops, Endless shopping and many many restaurants were all in walking distance. We can't wait to visit again! :) Great place for a family weekend get away !! :) 더 보기검증된 Expedia 이용 후기
"Excellent breakfast"
Good location - beside mall with places to eat. Breakfast was extremely good - lots of choices, staff friendly, constant cleaning and restocking.검증된 Expedia 이용 후기
"Very impressed"
Staff was very friendly and worked hard to impress. The hotel was well kept, modern (new), and nice. Clean pool and waterslide. Breakfast was good. Standard continental fare with eggs, bacon, and DIY waffles. Would definitely make this my first choice for return trips to Dartmouth.검증된 Hotels.com 숙박객 이용 후기
"dartmouth crossing - excellent"
great location, pool and staff are excellent. clean rooms, very affordable and close to airport, you can walk to shopping and food. highly recommend for business as its cheaper than downtown.검증된 Hotels.com 숙박객 이용 후기
Breakfast could be improved the quality of food was so so but there was lots there and good service. The room could use a bar fridge and maybe microwave.검증된 Hotels.com 숙박객 이용 후기
"would always recomend"
I have stayed at this Hotel many times it is in a good spot clean and very friendly parking is ideal I enjoy the buffet in the morning before going on my pleasure trip or business I find the staff very helpful and understanding검증된 Expedia 이용 후기
"Great hotel."
A solid hotel, well-organized, friendly staff. Rooms have always been quiet as the tomb - my person #1 priority. Check in/out was super fast.검증된 Expedia 이용 후기
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User loginNavigationTechnology On Your TimeInspired by O'Reilly Media's Make: magazine >> Also, be sure to check out craftzine. Search |
FreeCADShow & Tell - Mar. 5, 2013 Meeting time: 6:00 to 8:00 p.m. at the HMS Beagle Science Store, 180 English Landing Drive, Parkville, MO 64152 How to Create 3D Printed Parts Yourself.This month we will discuss the software options available that you can download to create your own 3D printed parts. Don't have a 3D printer of your own? Make:KC members will be able to access the group's printer to create your own parts. Show Us Your ProjectAs usual, bring your project out to show what you've been working on and share with our Show & Tell participants. Make:KC MembershipMake:KC invites you to come out and get involved with our activities but we depend on help from our community of Makers to keep the group and activities going. We are expanding opportunities with projects and workshops at the HMS Beagle Science Store. Membership with Make:KC has benefits; our members enjoy a 10% discount at the Beagle store on many items. We offer $25 annual memberships with Make:KC for individuals or $40 annual membership for families. Your membership helps support our research, project planning, outreach activities, and educational efforts.
Events & Activities
Make:KC SponsorsHMS Beagle Science Store - voted Coolest Store in Kansas City Make:KC AlliancesCheck out these other great Maker Friendly groups: YouthBlack Family Technology Awareness Association Professional OrganizationsLocal Hackerspace and Makerspace GroupsCCCKC Johnson County Library Makerspace Regional Hackerspace and Makerspace Groups |


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See By Chloe
Logo Tee
Launched in 2001, See by Chloe is a more relaxed and casual response to the success of the pieces to its elegant sister brand Chloe. See by Chloe has grown into a complete ready-to-wear line that has found favor by adding an urban edge to feminine tailoring.
- 100% cotton
- Styled with J Brand Mid Rise Skinny in Afterlife.
- Styled with gorjana Eden Stack Ring Set in Gold/Rose Gold.
- Styled with gorjana Lena Ring in Gold.
- Styled with CC Skye Double Header Pave Spike Bracelet.
- Modeled in size 40/S. See fit guide for model's dimensions.
- Revolve Style No. SEEB-WS67
- Manufacturer Style No. L 4 C47 02


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For those of you who were interested in the SP gathering, please check this thread out!
Holsti97 wrote:Top Ten Reasons I thought the Konvention was Kool:
jdzaharia wrote:Hey now!
White Butte isn't much of a mountain, but it is certainly more interesting than several other State HPs.
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SOURCE YourTango
New Survey Reveals Powerful Insights Into the Relationship Between Love and Food
NEW YORK, Oct. 4, 2013 /PRNewswire/ -- The verdict is in regarding the "300 sandwiches for an engagement ring" debate, thanks to a new survey by YourTango.com – the leader in love and relationships. It turns out you can be a feminist and still enjoy preparing meals for your partner according to 85% of respondents.
The survey explores the role that food plays in love. And while it confirms the way to a man's heart is through his stomach, this is true only for a slight majority of men. What's surprising is that nearly half of women indicated that food is the way to their hearts. The vast majority agreed that food plays an incredibly important role in their relationship.
"We were thrilled to discover that almost 80% of people felt preparing a meal for someone is a significant act of love!" says Andrea Miller, YourTango CEO. "Conversely, only 21% reported that food is a source of tension or conflict in their relationship, with 7% claiming they could not agree on what to eat. Less than 1% said it was because the person responsible for meals doesn't cook well."
In fact, 90% of people surveyed say a lack of kitchen know-how is not a deal-breaker for them.
Additional survey findings:
Through October 11, YourTango.com is hosting its second annual Breakfast, Love & Dinner campaign. Users can enter a sweepstakes for a chance to win prizes from Destination Dinners & Max Brenner's Chocolate Shop.
About YourTango
YourTango is the leading media brand dedicated to love and relationships. Our mission is to help our nearly 10mm unique visitors/month live their best love lives. Whether the topic is dating, sex, marriage, intimacy, divorce, or celebrity love, YourTango offers an abundance of entertaining, engaging text and video content, providing an active forum for this all-important, never-ending conversation.
*Survey methodology.
©2012 PR Newswire. All Rights Reserved.


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Who we are
Support CSE
CSE Bloggers
CSE in news
Director General of CSE and publisher of Down To Earth, an environmentalist pushing for changes in policies and practices and mindsets. More>>
I read newspapers and I watch the news unfold on scores of television channels. But in spite of these sources that keep me informed about current affairs, I would not know that floods are still ravaging vast parts of India. I would not know that over 2,800 people have died in these disasters, which have been termed as the worst ever in living memory.
I smelled the air of Bangalore last week. It was foul. I remembered how in the late 1990s, when Delhi's air was dark and dirty, we had run an advertisement in the newspapers: "Roll down the window of your bullet-proof car, Mr Prime Minister, the security threat is not the gun it is the air of Delhi." Since then Delhi introduced compressed natural gas, it increased the number of buses, it got better quality fuel. With all this, the air got less dirty and less toxic.
A journalist recently called me to check if I thought that India had the same food-consumer product record as China. He wanted to know if we face the problems that are plaguing Chinese exports of late: tainted pet food, toxic toothpaste, lead-paint in toys, chemicals in textiles.
It got me thinking: of China and of India and more importantly, about the phenomena we call the market.
The botted water industry is global in nature. But it is designed to sell the same product to two completely different markets: one water rich and the other water scarce. The question is whether this industry will have different outcomes in these two worlds. Or will we, for two opposite reasons, agree that their business costs us the earth and that it is not good for us?
Will Indian scientists measure up to the challenge of climate change? I ask this question because of the nature of the science as well as the nature of our scientists.
The government is being severely criticised for the wheat it is now planning to import. Rightly so. India's season for wheat ended a few months ago. When the crop was being harvested the government dithered on the price it would pay farmers; it floated tenders for import of wheat; it insisted on taxing the purchased wheat. At the end, farmers were paid Rs 850 per quintal, a price which included a 'bonus' of Rs 100.
Industrialist Ratan Tata has reportedly written to the prime minister cribbing about delays in implementing big buck projects. In his capacity as the chair of the government's investment commission he says over us $50 billion is tied up because of delays in allocating land and resources.
Some innovations change lives. A favourite of mine is the village milk collection system, a cooperative model. There's a dairy in the village, people bring in milk, the dairy in-charge places a sample on an instrument, checks the fat content, prints a receipt that tells the seller the fat content and the price.
My worst fears are coming true; and that has more to do with the politics of climate change than its reality. While concern on global warming reaches a crescendo, the world, instead of finding resolutions, is hurtling towards discord and dispute. Let us be clear: we do not have time to waste on bad politics and bad politicians.
Now that the reality of climate change has been accepted even by its strongest sceptics, there is a rush to find answers. The latest buzz is to substitute the use of greenhouse gas-emitting fossil fuels with biofuels—fuel processed from plants. Unfortunately, the way we are going about implementing this "good" idea could mean we are headed from the frying pan to the fire.


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Boiler Room |
Radiators & TrimGas Products |
Specialties |
Your new password is on its way. If you have problems accessing your account, please contact us at 1-888-757-4774 and we'll help you out.
I'm sorry, your e-mail was not found in our system.
If you think this is a mistake, please contact customer service at 1-888-757-4774 or via e-mail here.
You will have the option to create an account on the order confirmation page.
Honeywell Mixing Valves |
Honeywell Mixing Valves are available for residential and commercial applications.The Honeywell AM-1 series is the most popular series among Honeywell Mixing Valves. These valves accurately adjust, maintain and limit the hot water temperature to a desired setting selected by the user. In domestic water applications it offers scalding protection and bacteria growth control. By installing a Honeywell AM-1 mixing valve and raising water heater storage temperature setting and limiting mixed outlet water temperature to safe temperature more usable hot water is available. In heating applications it provides comfort and protects the equipment.
At PexSupply.com, our goal is to bring you the highest quality plumbing, heating and HVAC supplies at competitive prices. Our product catalog includes PEX Plumbing Supplies, Radiant Heat Supplies, VisionPro Honeywell Thermostats, Taco pumps and mini-split air conditioners. If there are items you are looking for but can't find on our website, please contact our Customer Service Representatives & we will do our best to find what you need.
© 2013 PexSupply.com


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Squeeze - Up the Junction: Essential Collection
NME.COM feature on Squeeze - Up the Junction: Essential Collection album including album review, artwork, tracks, listen now, tour dates, discography and more.
Squeeze News
Venue of first Squeeze gig to be honoured with 'blue plaque'
Unveiling will take place at Greenwich Dance Hall on March 23
Squeeze kick off final day of T In The Park 2009 in the rain
Band treat the early risers to the hits
Artist/Album artwork images hosted by Last.fm. For copyright enquiries please see here.


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You want to know which Autodesk products are supported on Microsoft Windows® 8.
Please check the system requirements for your product to verify compatibility and specific details (for example, some products are supported on the 64-bit version of Windows 8 but not the 32-bit version).
Unless noted otherwise in the system requirements for an individual product, Autodesk 2014 products are supported on the Windows 8 operating system.
Note: Unless indicated otherwise, this information applies to the 2013-2011 versions of all Autodesk products. Autodesk product versions of 2010 and earlier are no longer supported and are not included in considerations of Windows 8 compatibility.
Autodesk intends to support many of our key products on Windows 8 and will add additional products to the list of products supported on Windows 8 as updates and future versions are released. Product Support will provide its best effort to assist customers who have issues with products that are not currently supported.
The Autodesk products listed below have been tested and are confirmed as working correctly on Microsoft Windows 8 operating system:
Note: This technical solution will be updated as new Windows 8 compatibility information becomes available.
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Published date: 2013-Aug-14 ID: TS19048377