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Product Description
- Type: Digital TFT LCD
- Display Size: 3.5Inch
- Display Format: 4:3
- Power: ≤3W
- Power Supply: DC12V
- Video: 1 rearview video input/2AV input switch when reversing
- System: PAL/NTSC auto switch
- Contrast: 350:1
- Pixel: 480 x 234
- Installation: Install on the dashboard
- Operating Voltage: DC9V-16V
- Environment Humidity: 10%-90%
- Operating Temperature: -20℃~+60℃
- Storage Temperature: -40℃~+85℃
- Size: 104 x 103 x 25mm / 4.1 x 4.1 x 1.0in(L x W x H)
CCD Camera:
- Input Voltage: DC 12V
- Image Sensor: 1/4 inch SHARP color CCD
- Pixel Resolution: PAL: 500 x 582; NTSC: 512 x 492
- Electronic Shutter: 1/60~1/5000 Sec
- Lens Angular Field of View: 60 degree
- Effective Focal Length: 6mm, 9 Light
- Maximum Relative Aperture: F/2.0
- Resolution: 420 TV lines
- Video Signal Output: 1V(p-p) composite video signal at 75ohm termination
- Power Consumption: 1 Watt
- S/N Ratio: 40dB
- Cable Length: 50m/164ft
- The underwater camera system is designed to connect to your TV, VCR, LCD TV or some Digital Video Camera/Camcorders with an A/V Input
- Have provided "Gender Adaptors" in the case that your Digital Video Camera cannot accept male RCA plugs
- The underwater camera system is ideal for use on your home TV or VCR (when using Underwater Camera in the pool) or can be used on TV's found on cruise or dive boats,Show children what goes on at the bottom of the sea, lake, fishpond or aquarium
- Look down wells and tanks or search dams, rivers and flooded mine shafts. Search sink holes, look down pipe or large drain holes for missing pets or children. Marine departments can check for damage, inspect boats or search for wrecks
- Farmers can use the 50m underwater monitor to inspect the bottom of wells, tanks and dams
- Use underwater camera system to check drains and pipes for root/branches/leaf clogs and blockages
- Underwater camera system, multifunctional, can be used in many aspects of our dailylife
- The portable underwater monitor can be folded to be in the box, for easy carrying, also get great protection
- The camera can be used up to 50m under water, provides your clear pictures in any condition
- Come with a 50m-long cable, it is more convenient to use the portable underwater monitor
- The DC12V battery is used to power the monitor, give it constant power supply
Size in Detail:
How to Clean an Underwater Camera:
- As you learn how to clean an underwater camera, make sure to inspect all cover doors regularly, including the ones for the battery and USB port. These should be free from debris and anything else which may allow water to leak into the underwater camera
- Remove any dirt or debris with a soft, lint free cloth. Take care to also remove any drops of water with the same type of cloth. This should be done both before and after each use
- Inspect visually all waterproof casings and seals, to make sure that there are no cracks or tears. Make sure that all doors close properly, and that the camera is not damaged in any way. If there is visible damage, do not use the camera underwater until the casings are replaced
- Fill the bucket with fresh water, making sure that it is free from any salt or other chemicals, as this could cause damage to the camera
- Close all doors on the camera, making sure that it is securely sealed. Immerse it into the bucket, with the lens facing downwards
- Open and close the lens cover a number of times, this is usually done by pressing the power button. This will make sure that any dirt or other obstructions are removed, preventing the lens cover from becoming stuck or damaged
- Remove the underwater camera from the bucket of water and gently shake off any excess liquid. Dry it using a clean cloth, then leave to air dry with all of the doors open. Now you know how to clean an underwater camera
Package Included:
- 1 x CCD Underwater Camera
- 1 x Underwater Monitor
- 1 x 6500mAh Battery
- 1 x Battery Charger
- 1 x 50m Cable
Customers' Reviews
Get DinoPoint™ by Writing reviews, Post Images, or Post Videos. The first five customers can get double points!
from (Perth Western Australia)
Bought this item on
06-22-2011- Pros: The price, size is compact
- Cons: none
- Other Thoughts: I liked that the unit looks like in the picture and that it came so quickly.Had fun getting it to work, screen info abit confusing does need power to get it to work.
Was this review helpful?
Jimmy Boucher
Bought this item on
03-12-2011- Other Thoughts: really great Portable Underwater Monitor, it is what I am looking for, like it very much
Was this review helpful?
Pierre Menard
Bought this item on
02-28-2011- Other Thoughts: Product is exactly how it says. very clear picture and seems durable. thank you amazing product idk why i waited so long to buy one!
Was this review helpful?
Paul Murphy
Bought this item on
02-06-2011- Other Thoughts: I like the Underwater Camera System, I can used it in many places, very powerful in functions
Was this review helpful?
Carole Van Camp
Bought this item on
01-17-2011- Other Thoughts: Nice big screen, great color (even in the sunlight) and great detail on the fish that it sees. The charging system is sort of a pain to do and it could use more room for the cable and the "eye". Over all, very happy. Worth the extra bucks from the cheaper ones...
Was this review helpful?
Wayne Lucas
Bought this item on
01-10-2011- Other Thoughts: This Underwater Camera System looks very stylish and appears to be durable, little pricey but worth it.
Was this review helpful?
Keegan Barrett
Bought this item on
12-22-2010- Other Thoughts: I like to buy goods on the Dinodirect because every time I come here, the servers offer me very good service. And of course, the products them sole to me were always good. I will surely buy things here.
Was this review helpful?
Kevin Tam
Bought this item on
12-13-2010- Other Thoughts: One of the best 50m Underwater Monitor that I've owned ever. Very solid and sturdy! great as it should be! highly recommend!
Was this review helpful?
John Silber
Bought this item on
11-28-2010- Other Thoughts: This Underwater Camera System can really get 50m under the water, I used it to fish in the lake of my town. and the pictures under 50m are also clear. Great Underwater Camera System!
Was this review helpful?


text:
Do you ever wonder why some organizations have their calendars booked and service appointments scheduled months in advance, while others are surviving on day-to-day appointments, dependent on break-fix calls to keep them afloat? Obviously, it's better to work for an … Continue reading
Technology Solutions to Boost the Health and Growth of Your Organization
This post is contributed by MSI Data partner, Viewpoint Construction Software. The first of the year offers many opportunities to take a fresh look at where important matters stand, and where they stand for improvement. If you're a business owner … Continue reading
5 Field Service Resolutions to Jumpstart 2014
Here we are already. It's December and the holiday season's in full swing. We're all busy rushing around, getting food, decorations, and gifts ready for parties, preparing to enjoy some much anticipated time with family and friends. I love this … Continue reading
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Have you been searching for a career with just the right mix of customer interaction and software experience? Join our team at MSI Data and see how you can let your software expertise shine while engaging with customers in an … Continue reading
3 Ways Mobility is Changing Field Service
I'm sure enterprise mobility is a phrase you've read about a hundred times. Everyone from hobbyist bloggers to major news sources are weighing in on the topic and everywhere you look, businesses are frantically trying to evaluate how they can … Continue reading

subset: commoncrawl


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We had a great stay at GV Habitue, one week in september. The perfect location, nearby restaurants, subway, deli, all the cosy neigborhoods in Manhattan. What more can we ask for(?) The apartment was also very nice, cleaned almost everyday, very comfortable bed, spacious. The hosts are amazing, Kevin was telling us the most important info when we arrived, and... More
- Also Known As:
- a Greenwich Village Habitue Hotel New York City
- a Greenwich Village Habitue Hotel
- Official Description (provided by the hotel):
- Owner occupied 1830's Federal style Brownstone has 2 completely private, self-contained, fully appointed apartments for rent - single or double occupancy ONLY - 7 night minimum stay. ... more less


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Less Than Dot is a community of passionate IT professionals and enthusiasts dedicated to sharing technical knowledge, experience, and assistance. Inside you will find reference materials, interesting technical discussions, and expert tips and commentary. Once you register for an account you will have immediate access to the forums and all past articles and commentaries.
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How important is database documentation to you? I know you have a hundred "more important" things to do. But consider this: how much time is it going to take you to document the purpose of a constraint right now? How much time will you spend trying to r…
Take the First Step
Earlier this month I attended PASS Summit 2013 in Charlotte, NC. It was my third, and my favorite so far (I say that every year). Among the many activities I got to participate in, I was interviewed for PASStv. (Look for me on day one!) One of the ques…
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It's almost time for the biggest SQL Server focused conference in the world – PASS Summit. I'll be heading down to Charlotte, NC for a week of learning, teaching, running, and networking!
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SSRS has a default set of color combinations for charts. Let's face it, they aren't pretty.
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This month, our host, Bradley Ball, wants to hear about at least one moment in SQL time that I would like back.
Book Review: Pro SQL Server 2008 Failover Clustering
This is the most comprehensive look at Windows and SQL Server failover clustering that exists. In this book, Allan Hirt, a clustering expert and Microsoft MVP, lays a solid foundation for all aspects of clustering.
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I love and am very familiar with SQL Server. But, as a consultant, I need to step outside of that box pretty often, and advise people on applications, storage, and networking. I have general knowledge of those topics, gathered from many years in IT, see…
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On Saturday, April 6, 2013, Madison, WI hosted its second annual SQL Saturday. It was another successful year! We had 241 attendees, an increase over last year. It's been exciting to watch the Wisconsin SQL Server community grow over the past five ye…
T-SQL Tuesday #41 - Presenting: Be Prepared
It's the second Tuesday of the month, and you know what that means: it's T-SQL Tuesday! Standing up in front of a room of people and talking, especially on a technical topic, can induce nightmares. Think about everything that could go wrong. Your lapto…
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The Official "Aaron Rodgers put my fantasy team on his back" thread (self.fantasyfootball)
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[–]BattaUp 2 points3 points4 points 1 year ago
Sounds like a Guppy League
[–]dubblechzburger 0 points1 point2 points 1 year ago
Yeah it's one my good friend made a while while back and asked me to join. It's definitely different but it's still fun using a different strategy to try and win that league.
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Automotive Digital Marketing Professional Community
ADM serves Car Dealers, Automotive Marketing Pros and Internet Sales Managers
Facebook has reached over a billion active users and as our social history continues to unfold, each of our activities gathers quite a bit of intelligent information once it is formulated into grouped statistics. Wouldn't you be surprised to find out that though many complain about their privacy being infringed upon by Facebook that only 5% of Facebook users have set their privacy settings to be completely private? Don't you want to know what our most popular activities are? Or that though it seems like every time we turn around we are poking someone back but that 63% of Facebook users have never used the "poking" feature?
Those of us who use Facebook have deep rooted emotions about it, whether we love the social site or hate it; we are most certainly on it and use it various times throughout our day. In the following infographic learn more about our time spent on Facebook and statistics that may surprise you.
Welcome to
Automotive Digital Marketing Professional Community
Please use the "Sign Up" link above to complete your registration form and become a member of the industry's leading Automotive Marketing and Internet Sales Professional Community. ADM members have access to resources, connections and private events that provide them with a competitive advantage.
Social Network for Car Dealers, Automotive Marketing, Advertising and Sales Management Professionals sharing Internet strategies and tactics
December 12, 2013 from 8am to 5pm – Indianapolis, IN
0 Comments 0 LikesDecember 12, 2013 from 12pm to 1pm – http://www.dealeron.com/webinar/events/how-to-become-a-rockstar-salesperson/
0 Comments 1 LikeDecember 12, 2013 from 1pm to 2:30pm – https://www4.gotomeeting.com/register/8769
0 Comments 0 LikesDecember 13, 2013 from 10:30am to 10:45am – Nationwide
0 Comments 0 LikesDecember 19, 2013 from 12pm to 1pm – http://www.dealeron.com/webinar/events/killer-seo-strategies-for-2014/
0 Comments 1 LikeDecember 20, 2013 all day – Cincinnati, OH
0 Comments 0 LikesJanuary 16, 2014 from 12pm to 1pm – http://www.dealeron.com/webinar/events/unleashing-the-power-of-youtube-and-video-to-sell-more-cars/
0 Comments 1 LikeADM Professional Community Blogroll:
1. Social Media Optimization
3. Persuasive Concepts Social Marketing
11. Weed Out Losers and Hire Winners
12. Take Control of Dealership Reputation
Get listed on this Blogroll; send your blog title text and your blog's associated URL. Include a link to your site's page showing the ADM title text: "Automotive Digital Marketing" linked to: http://www.automotivedigitalmarketing.com to Ralph Paglia via ADM.
© 2013 Created by Ralph Paglia.


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By PR Newswire | Article Rating: |
September 12, 2013 04:00 AM EDT | Reads: |
459 |
LONDON, Sept. 12, 2013 /PRNewswire/ -- Kentico Software, a leader in customer experience management, today announced the kick-off of Kentico Connection Europe in London on October 7-8, 2013.
(Logo: http://photos.prnewswire.com/prnh/20130530/SF23337LOGO)
Kentico Connection Europe is a two-day conference for developers, marketers, partners and business owners looking to derive maximum value and return from their marketing efforts. The show features three tracks—Technology, Partner and a new Marketing Track that attendees can register separately for—together delivering more than 30 sessions on up-to-the-minute trends, industry insights, and partner case studies that demonstrate the level of sophistication made possible by the right combination of marketing tools, creativity and expertise:
- Technical Track: Kentico-led informative technical sessions, as well as insights into the powerful digital marketing tools available to create sophisticated marketing programs.
- Partner Track: Comprised of case studies and best practices for solving real-world marketing challenges.
- New Marketing Track: An intensive and content-rich program for marketing professionals interested in staying ahead of the game. Learn the secrets of digital marketing with the latest knowledge and best practices from a host of industry experts.
"Digital marketing is about more than just technology," said Petr Palas, founder and CEO of Kentico Software. "It's also about creativity and know-how. That's why this year's Kentico Connection Europe conference promises to be the best yet, with a new Marketing Track featuring insight from some of the most experienced and cutting-edge professionals in the business."
Attendees of Kentico Connection Europe can expect new Marketing Track sessions focused on a variety of marketing disciplines and channels, including:
- Branding: Why Do 90% of Campaigns Fail? by Martin McInnes, The Church Agency
- PR: Content and Collaboration: The New Global Marketing Opportunity, by Mary Shank Rockman, MSR Communications
- The Rise of Social Photography and What It Means That Your Customers are Communicating in Images Instead of Words, by Dirk Singer, The Rabbit Agency
- Mobile: The Future of Content Management When the Mobile Broadcasts Intent, by Tony Fish, FeynLabs
Kentico Connection Europe is part of Kentico's annual Connection Series, which also includes Kentico Connection Boston on September 23-24 and Kentico Connection Sydney on November 11-12, 2013.
To register and for more information on the Kentico Connection conferences in Boston, London or Sydney, please visit: http://connection.kentico.com
About Kentico CMS
Kentico CMS is an enterprise Web Content Management System and Customer Experience Management solution that provides a complete set of features for building websites, intranets, community sites and e-commerce solutions on the Microsoft ASP.NET platform, on-premise or in the Cloud. It supports mobile websites, SEO, document management, online marketing tools, multilingual websites, and multisite management, and comes out of the box with 70 ready-to-use modules, 400 configurable web parts, and source code available by request.
About Kentico Software
Kentico provides an affordable, award-winning integrated marketing solution that's easy to use but also highly customizable for powerful real-time customer-centric marketing across multiple channels, on-premise or in the Cloud. Founded in 2004, Kentico is headquartered in the Czech Republic with offices in the US, UK, and Australia. A Microsoft Gold Certified Partner with 1,200 digital agency partners, Kentico powers more than 16,000 websites in 90 countries. Customers include Bacardi; Great British Chefs; Gibson; Mazda; Microsoft; Mighty River Power; Run and Become; Sony; Vodaphone.
All product and company names herein may be trademarks of their respective owners.
Published September 12, 2013 Reads 459
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- Cloud Solutions and Technology
- Part II: EMC announces XtremIO General Availability, speeds and feeds
- EMC announces XtremIO General Availability (Part I)
- Acquia Appoints Martyn Eley as General Manager of EMEA Operations
- WSO2 Carbon, WSO2 API Manager and WSO2 Governance Registry Increase Speed, Scalability and High Availability Through Hazelcast
- Why Small Business Needs Cloud Computing
- Opening Keynote at Cloud Expo | The Changing Atmosphere of Cloud Computing
- U.S. General Services Administration Moves Order Management System to IBM SmartCloud for Government
- Cloud Computing 2.0 Flexibility Discussed at Cloud Expo
- Cisco Web Security Webinar: Ensure World Class Protection
- Storage Made Easy Announce the General Availability of Their On-Premise Enterprise File Share and Sync Product
- Establish and Manage a Recurring Revenue Model
- OneLogin Raises $13M to Power Expansion
- Financial Results, New Appointments, and General Meetings - Research Report on Red Hat, Yelp, Sierra Wireless, Infinera, and Bitauto
- Cloud Solutions and Technology
- At-a-Glance: Comparing VMware vSphere 5.5 & Windows Server 2012 R2 Hyper-V
- Part II: EMC announces XtremIO General Availability, speeds and feeds
- Fathers of Clouds – A Tribute
- EMC announces XtremIO General Availability (Part I)
- Acquia Appoints Martyn Eley as General Manager of EMEA Operations
- WSO2 Carbon, WSO2 API Manager and WSO2 Governance Registry Increase Speed, Scalability and High Availability Through Hazelcast
- WebRTC Summit | WebRTC Business Models: Building a Web-Based Telecom Co
- Open Server Summit Reports Doubling of Registration
- Why Small Business Needs Cloud Computing
- The Top 150 Players in Cloud Computing
- Where Are RIA Technologies Headed in 2008?
- Six Benefits of Cloud Computing
- FullArmor GPAnywhere Secures Microsoft Application Virtualization Applications Through Group Policy
- SYS-CON's Virtualization Conference & Expo: Themes & Topics
- SYS-CON's Virtualization Journal Opens Its "Readers' Choice Awards" Nominations
- "Virtualization Is Now a Key Strategic Theme," Says Citrix CTO
- Application Virtualization: Instant Migration to Vista, Fast Delivery, Secure Access, Side-by-Side Deployments
- The Top 250 Players in the Cloud Computing Ecosystem
- Application Virtualization
- Integration with Windows Vista, Microsoft Excel, and Microsoft Application Virtualization
- What's the Difference Between Cloud Computing and SaaS?


text:
We run org.eclipse.ant.core.antRunner to build our plugins and RCP projects. In the build logs we get a ton of messages like:
[eclipse.buildScript] Bundle org.eclipse.X:
[eclipse.buildScript] Another singleton version selected: org.eclipse.equinox.X_1.0.4.v20081112-1019
The reason is clear; There are two different versions of a particular bundle and it chose the latest. We cannot change the Eclipse installation to remove the older plugins, so what can be done to get rid of these messages?
Bonusquestion: What class prints out these messages? One option could be to create our own version where these messages are never shown.
Stackoverflow suggested this question:
From there I found this page:
which contains more info of ant logger and listeners.


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A subreddit for rage comics
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I'm still in here (i.imgur.com)
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[–]Capzo -5 points-4 points-3 points 12 months ago
Comics must be between 2 and 8 panels.
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Every time this rule gets posted, the commenter gets downvoted. I feel like this comic should be an exception, even though crappy, it's still low in context.
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Below are top resources on SparkPeople for Diabetes Disease.
Diabetes is a disease that can be prevented in most cases and treated successfully in the rest. But improper management of this disease can cause serious complications in many different areas of the body. If you have diabetes or are at risk for devel... Read more
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Diabetes is a chronic disease that affects more than 23 million people in the United States. If managed well, people with diabetes can live normal, healthy lives. However, the many devastating effects of uncontrolled or poorly controlled diabetes are... Read more
What Is Diabetes? Diabetes is a condition that causes high blood sugar levels. It is a chronic disease that can be managed but not cured. It does not go away. Knowing as much as you can about the disease is the first step to managing it effectively... Read more
Before developing the serious health condition of type 2 diabetes, a person will almost always have pre-diabetes beforehand. But pre-diabetes is a condition without symptoms, meaning that many people can have it without even knowing it. Left unchecke... Read more
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hi i am peggy,i am new here and would like to make some news friends to see if we can help each other reach our goals, i am a diabetic and my BS are all over the board,my diabetic dr has changed my insulin amounts 4 times in 2 months now, so now i h... Read more
I was wondering if anyone has followed this eating plan or is following this now. It is a healthy eating plan for everyone not just diabetics.... Read more
I don't see a lot on this here.... Read more
So, I was diagnosed as pre-diabetic in my teens. I am now almost 40. Several years ago I began taking a medication which pushes a lot of people into diabetes, even if they are not predisposed. I must take this medication to stay alive and to be fu... Read more
Gum Disease is linked to heart disease, strokes & diabetes. High glucose levels in saliva promote the growth of bacteria that cause gum disease or infection and even tooth loss. Make sure to floss daily, brush after each meal and if your throat is d... Read more
The science magazine Natures said Virgin Oil Oil may have the same effects of Ibuprofen. It may also inhibit the oxidation of LDL (bad) cholesterol. A tsp per day may help in treating arthritis, cardiovascular disease, high cholesterol,& in pain ma... Read more
We are Managing Our Diabetes! And we will get it under control with good healthy eating and exercising, and the support of our Spark Friends! Come Join Us in your Quest to Become Healthy!... Read more
Diabetes is a serious condition that can affect the heart, nerves, vision and other vital organs. But did you know that diabetes might also increase the risk of Alzheimer's disease, and even increase the speed of progression once the disease strikes... Read more
Diabetes mellitus is a group of diseases characterized by high blood glucose levels as a result of defects in the body's ability to produce or use insulin. In 2006, diabetes was the seventh leading cause of death according to U.S. death certificates.... Read more


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Thursday, December 19, 2013
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Help the Philippines Typhoon Relief effort
Note: Select a region before finding a country.
A passing shower or two
Partly sunny, a shower or two
Partly sunny with a shower
Dec 6, 2013; 4:48 PM ET A snow storm marches into the Northeast this afternoon first with sleet and rain then with heavy snow.
6:35pm AST


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English: Media relating to Bellinzona railway station, on the Gotthard railway in the Swiss canton of Ticino. For more information, see the wikipedia article Bellinzona railway station.
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Tarragon flavored vodka, along with sweet grass (Hierochloe odorata), dill or thyme vodka infusions,...
To spice up the classic cocktail, whip up a Limoncello recipe with little more than lemons, sugar an...
Choosing the ideal products for your skin type can get complicated. Take the guesswork out of your b...
Homemade liqueurs can also be made with sage or fennel leaf.
Makes 1 pint Italian herb liqueur is a sweet combination that's full of flavor.
Leah A. Zeldes is food editor of Chicago's Lerner Newspapers.
Click here for the original article Homemade Liqueurs with Herbs.
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Alleged burglar apprehended after call from Fair Lawn resident
Fair Lawn - A resident's call to police resulted in the arrest of a suspected burglar.
On Oct. 17 at 4:28 p.m., Detective Peter Yuskaitis, Detective Jeff Welsh, P.O. Eric Eleshewich, P.O. Nicholas Snyder and Sgt. Eric Leitman responded to the area of Morlot Avenue near the Morlot Avenue Bridge after a resident called police to report a male acting suspiciously as he was walking toward Paterson carrying two bags, said Sgt. Brian Metzler in a press release from the Fair Lawn Police Department.
The police department had previously alerted residents in that area about a string of burglaries that had occurred in July and August. They had been asked to report anything they deemed to be suspicious.
Osvaldo Rodriguez, 38, of Paterson, was located with assistance from Paterson Police Department. He was arrested based on the description given by the Fair Lawn resident, according to police.
Rodriguez had a Spider-Man backpack and a black bag with musical notes in his possession. The items were determined to have been taken from a residence on 1st Street near Morlot Avenue. The property recovered in the bags included a laptop computer, a camera, an iPad, an electronic reader, a cell phone, jewelry and credit cards.
Rodriguez was charged with burglary and theft. Bail was set at $50,000 and he was sent to Bergen County Jail. Rodriguez was also arrested in Paramus on Sept. 9 on charges of burglary, said police.
Detectives are continuing the investigation because Rodriguez is considered a person-of-interest in other burglaries, according to police.
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Ginsu 14 Piece Stainless Steel Knife Block Set GinsuSale Price $52.99
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India's monopoly regime stops us from making many drugs here: ...Cipla's market is not India alone and it has to go global, says Yusuf K. Hamied, who stepped down as MD in February C.H. Unnikrishnan Tweet Tweet 0 Subscribe to: Daily Newsletter Breaking News Latest News 12:24 AM IST 12:22 AM IST ... Livemint.com, 3 weeks ago |
1 images for "yusuf k hamied" | ||||
Cipla to expand its international drug market gloaballyMUMBAI -- Having made its first foreign acquisition earlier this year, Cipla Ltd, India's second largest drug maker by market share, is planning to go global on the back of its reputation as a maker and supplier of affordable generic drugs to some ... MedCity News, 2 weeks ago
Cipla plans bigger global presence [Mint, New Delhi]
BusinessWeek, 2 weeks ago
'India is committing selective genocide in healthcare'The recent release of the documentary Fire in the Blood has revived the debate on whether patents block access to life-saving medicines. Dr Yusuf K Hamied, chairperson of Cipla and the 'star' of the film, made international headlines in 2001 when he ... Times of India, 1 month ago
Compelling StoriesK atiyabaaz is a documentary that could have been a feature film. Colourful characters in search of better destinies, their personalities, traits and quirks, conflicts and clashes and triumphs and failures tell a unique, compelling story which ... Outlook India, 1 month ago
Film review: Fire in the BloodAReduce font size AIncrease font size AReduce font size AIncrease font size URL copied to clipboard ApnaCircle My Saved articles RELATED Film: Fire in the Blood Director: Dylan Mohan Gray Cast: Bill Clinton, Zackie Achmat, Peter ... Mumbai Mirror, 2 months ago
Cipla tries to change course, eyes markets beyond USDrug maker Cipla plans to shift its strategy, on both markets and products, to address issues on competition and revenue. It has been known for for selling low-priced and for challenging the patents of multinational corporations. Now, it plans to ... Business Standard India, 2 months ago


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Presidential Tie Clips
Nobody Reports
The fight in Washington continues, and the Republicans seem to be caving into the lies of the current President, Barack Obama, who has viciously attacked the "tea party" members of the house, if they don't give into his full demands. Obama blames the tea party for causing the United States to default for the first time in history. Many of us out here in nobody land, cannot believe our ears…
Because if we do default, it will be by Obama ordering the treasury not to pay the bills. (And with Dr. Lew, he has an obeying lap dog.)
Once again the democratic rule: Do the crime, blame the other guy for it.
Would a President do this impeachable act just to further destroy his enemy, the Tea Party? (In case you're late to this, the Tea Party are the last Americans standing who want Constitutional rule.)
Of course he would. That's what happens when you let a President get by with murder. Congress has far too many times, looked the other way when their Presidents commit unconstitutional crimes.
Impeachment is almost impossible now after the Monica Lewinsky debacle. Bill Clinton should have been impeached when he joined up with our giant military manufacturers to give China our technology. He should have been impeached for giving North Korea plutonian. They could have impeached him for lying under oath. Instead, they made the impeachment about sex, god forbid they cut off their own future campaign coffers. And by doing impeachment on such a trivial matter, they put the country through so much negative emotion about the whole process, we will more than likely never see it supported ever again.
Maybe that was the idea.
And so, with each Presidency the office itself has grown stronger, which is what the elite in the circle we call Washington and Wall Street want. it truly is about the rich against the poor—–a one party system, with one strong leader at the top.
I came upon this picture today. It was taken right before then, President George H.W. Bush, was going to send the troops into combat in the first Gulf War. He kept saying it was to save the poor helpless Kuwaitis from that mean neighbor, Saddam Hussein.
He could have told us that we had struck a deal with the Saudi's to protect them in exchange for bases on their land, but that wouldn't sell to us nobodies, who they consider to be better off ignorant of all that they do.
What struck me about this picture…is the expression on his face. And the grace of his hand, as if, he is a Napoleon in history, reaching out for eternity saying. "GO…FREE MY PEOPLE!…YOU ARE MY BRAVE AND HONORABLE ARMY FOR GOOD!"
In fact, the Times editor asked Diana Walker, the photographer who took this picture, to explain it.
"It looks like he's Moses parting the waters."
BS. According to Diana, he was standing on a wooden box and throwing out tip clips. (I.e…he was posing for her)
Wow. Tie clips. Just what you need when you are about to be shot.
That reminds me of all the universal corporate "honor" Christmas meetings that I've been to, where the bigwigs of corporations would hand out plagues for "most sales." instead of what most people would have preferred; money.
Anyway, Diana Walker, waited all day to get that shot. The President had been surrounded the whole day by Congressmen who wanted THEIR picture taken with the President. (Speaker of the House Thomas Foley is in the bottom left hand corner walking away.)
Diana got more than a tie clip for this picture. She won the World Press Photo competition. And I'm sure the "President" saw to it that she got that reward. No doubt, this is one of his favorite pictures.
It will be interesting to see what will happen in the next few days. If the House Leaders give into the President, we will have a one-party system.
If they don't give into the President, we will still have a one-party system, with a 'benevolent' dictator at the top, who if you are lucky, will excuse you from Obamacare.
The rest of you…will just have to go to into battle without that tie clip.
Buddy Did NOT Have His Own Plane….Sad.
Nobody Reports
Ahh….there's NOTHING like having a tax-free charity to support your lavish travel expenses, is there? Why else do you think all the rich set up the NGO's?
And today, somehow, the Clinton's travel expenses were released—by Edward Snowdon.
No, I'm kidding.
No doubt the Clinton's want to get all the unpleasant stuff out-of-the-way so we'll forget all about it by-election time, where Hillary will probably be compared to Mother Teresa, The Virgin Mary, and the first gay running for office. But she will stay married to Bill because he has done so much for the world.
WASHINGTON – Bill Clinton's foundation has spent more than $50 million on travel expenses since 2003, an analysis of the non-profit's tax forms reveal.
Yes, it takes millions of dollars to travel to Rwanda, just to give a village a $50 well, and an important photo-op for Chelsea, who is now warming up for her mother and taking over the family business of learning how to live off other people's money.
The web of foundations run by the former president spent an eye-opening $12.1 million on travel in 2011 alone, according to an internal audit conducted by foundation accountants. That's enough to by 12,000 air tickets costing $1,000 each, or 33 air tickets each day of the year.
That overall figure includes travel costs for the William J. Clinton Foundation (to which Hillary and Chelsea are now attached) of $4.2 million on travel in 2011, the most recent year where figures are available.
Bill Clinton of course misses Air Force One, so he likes to fly around in a Boeing 727. Who wouldn't? Leave it to the Clinton's to not pay for ANYTHING in their lives. The 727 is donated by a rich billionaire donor.
I need to get one of those.
BUT…next to the Obama's, the Clinton's look like Middle Class yokels. Obama has spent $1.4 billion dollars running around, and everybody knows, it goes up every second. Not to mention, they got another dog for BO…a girl named Sunny, because its gets lonely being the only dog on HIS own private jet. They might have to get a bigger jet for the dogs. In fact, that's the plan, don't you think? Puppies…to rationalize the extra plane?
On the other hand, we must ask ourselves: Will Hillary's travel expenses be even more than Obama's if she becomes President?
You bet. Bill will need another Air Force One, and Chelsea will need a Boeing 727.
All they need to do is get some more pets, because Bill's last dog… Buddy…well. You don't really want to know what happened to him. Let's just say, he didn't get his own plane, and it's a shame. Because if Bill had gotten Buddy his own plane, then he would still be alive, because Bill left him at home with Secret Agents, and Buddy got run over by a car, even though he was being watched by two very expensive baby sitters courtesy of the taxpayers.
There's a moral to this story here…I'll let you find it. I can't.
King George…NOT the Baby….
Nobody Wins
While EVERYONE was gushing today about how X-President George H.W. Bush shaved his head bald to honor a small child of one of his secret service members who had Leukemia— I was astounded to see all those guys standing behind him. No, ……I was—amazed and shocked that he needed all those guys in this picture to protect him. I always thought they got one or two for the rest of their lives, not 100. I tried in vain to find out just how many secret service are guarding our X- Presidents and kept coming up with the same answer: Most X Presidents have a total of 56 agents, but George Bush Jr. has 103.
Come on. If you believe THIS, we are paying $2 trillion a month for these guys to live in grand style with no fear? One hundred agents a piece?
Is it any WONDER we are going broke?
They get 2 trillion a month and get assigned 100 secret service people each. They also get their kids to go to president schools like Adams family did and Bush.
No wonder they spend billions to get into the White House. Please…somebody tell me this is NOT true. Awfully nice of King George to show compassion, but maybe the taxpayers should be asking…why don't you show a little compassion to the taxpayers? Do you really need an army?
Let's All Go to Africa and Celebrate Women!
Nobody Reports
Barack Obama will met up with former President George W. Bush while they are both in Tanzania. Good old George is going to help polish up Obama's lame legacy at Benghazi by standing with him at a wreath-laying ceremony in honor of the 1998 bombing there that killed 11 people at the U.S. Embassy. George is there to promote African women. Michelle Obama and Laura Bush will also team up at a conference on African Women organized by the George W. Bush Institute. If they think this is going to help get Jeb Bush the black vote in 2016, they need a Tanzanian transplant.
In September, Jeb Bush, will be giving the 2013 Liberty Medal, to Hillary Clinton, in Philadelphia.
He had this to say about Hillary:
"Former Secretary Clinton has dedicated her life to serving and engaging people across the world in democracy," the former Florida governor said in a statement announcing the award. "These efforts as a citizen, an activist, and a leader have earned Secretary Clinton this year's Liberty Medal."
The Bush family must expect Jeb to win the White House, and they are just handing out some goodies to Obama and Hillary as consolation prizes: OR…Jeb and George are just really nice guys.
Nobody Thinks if the Republicans really want to win back the White House, the last thing they should be doing is helping their opponents.
I would rather see Presidents putting wreaths on the graves of the recent firefighters that lost their lives in Arizona, but, that's me. I can't wait to see what' Obama's "institute" is going to be, but one thing we all know, the x Presidents make more money out of office than in. Which is why they are willing to spend billions to get the job.
Nobody's Perfect: Edward Snowdon VS The United States Presidents
Insanity: doing the same thing over and over again and expecting different results.- Albert Einstein.
Nobody's Perfect:
This week we have the NSA whistleblower, Edward Snowdon, VS the United States Government.
Let's start with Edward Snowden.
A young man does an interview with the British paper, The Guardian, and tells the world that our government is spying on everything we do. He believes it's wrong, and even though he knows he will be hunted down, and possibly killed, he did it anyway. After that statement, the man just disappeared. Snowden, and this is very important, allegedly downloaded the country's most sensitive secrets, especially how the U.S. Government does surveillance abroad, so other countries would know how we spy on them. If it's true, that's not good, although I don't for a minute believe that the rest of the world is that stupid, and didn't know this anyway.
Since we haven't heard from the man, we have no idea what the truth is. John Kerry, a man who lied big time about his own involvement in Vietnam, and who branded American soldiers as war-criminals when he was younger, said that "people may die as a consequence of what this man (Snowden) did."
And, in a "I told you so" Nobody smirking moment, (Read yesterday's post) John Kerry, our defense secretary, also said this:
"It is possible that the United States will be attacked because terrorists may now know how to protect themselves in some way or another, that they didn't know before.,"
Life is wonderful when you can blame some insignificant nobody, isn't it?
And then, we have The United States government:
When it comes to protecting the secrets of the United States, I think the Democrats make Edward Snowdon look like an amateur.
Take Bill Clinton. His North Korea appeasement program included a nuclear reactor. He also provided North Korea with detailed satellite photos of Kadena during the 1990′s as part of an "earth resources" deal with China. The U.S. Dept. of Commerce documents show the Chinese satellite "remote Sensing Center" was supplied with "world class remote sensing data acquisition, processing, archive and distribution" equipment. The state of the art satellite equipment was proved by Huges Corp.
In 1996, President Clinton personally signed an executive order releasing a wide variety of space and missile technology for export to China. That executive order was supported by the CEO's of Lockheed, Loral, ad Huges and it allowed China to purchase sophisticated electronic guidance and control systems for its military missiles.
Remember: China helped Pakistan nuke up, and Pakistan helped North Korea, Iran and Iraq. All because Bill and his big military companies, wanted to make some money. In the meantime, he was encouraging all other nations, like Japan and India to not nuke at all.
So, Bill Clinton has made it possible for China to be able to nuke the United States. Any city. Any time. And now, North Korea. And yet, he is still adored.
That was treason of the highest order. No other way to look at it: Treason.
What else happened during Bill's reign? Remember the Los Alamos fire? Opps, a Chinese spy got in and downloaded everything. In fact, in the last ten years that I have been reading about politics, I can't count the number of times our secrets have been stolen. Remember when the head of the CIA was our biggest informer to Russia? Who let THAT happen? If this program is so damn good, how come we do not stop China from stealing all our good stuff?
And then you have the current President, who has continued to lie. He continues to fundamentally change our now, socialist America, into a soon- to -be communist dictator. He is flooding our shores with Muslims, and his cabinet with Muslim sympathizers. He let men die in Benghazi, sent the IRS after patriotic Americans, pushed Obamacare through in the middle of the night, and that's only what we know of.
Who is the bigger monster here? Do we let him pass because we honor the office, even if it means the office is destroyed and replaced with a dictator?
So, who wins the Nobody's Perfect award for the week?
Is Edward Snowdow out to kill innocent Americans? Or, do you trust the government that rules you now?
If you choose our government, then you are doing the same thing over and over again and expecting different results. Snowdon, might be a lone traitor, we won't know unless we hear him speak. BUT—Our own government has hurt, and will continue to hurt more of its own citizens that Edward could ever dream of hurting. You have to buy the 'facts' that everyone is telling you, that he intends to give all our secrets away.
And if he is a "traitor" is he any worse than a President that gave nuclear capability to China?
To say the Edward Snowdon is a bigger traitor than Bill Clinton or Barack Obama is insanity. And to keep trusting the criminals in office, is just more insanity. But that just my lonely Nobody Opinions.
(Information taken from Newsmax articel: China Acquires Missile Technology– Charles R. Smith, May 17, 2007)
Remembering Our Presidents
Nobody Remembers
Once again, the timing of the dedication of x-President, George W. Bush's library, couldn't have had come at a more propitious time. The nation had just been attacked by Muslim radicals, giving the Bush family a platform to remind us all, that Bush kept us safe throughout the rest of his Presidency after 9/11. And in this light, it was okay for George himself to push for another Bush in the White House: His brother, Jeb.
As I watched the former Presidents all standing, and then the speeches, I can't say that any one of them talked about what THEY had done for our country. It was all about how they, while doing the 'toughest' job in the world…changed the world, and plan to keep on doing it.
Not much about helping America. No mention of the hard times we are going through now.
I posted Jimmy Carter's speech, simply because to me, what all of the former Presidents seem to be most proud about is what they have accomplished in other countries. That's all they bragged about in all their speeches. According to the former Presidents, Bush's best legacy was his work on aids in Africa. Clinton, Carter, and Obama all mentioned it.
George W. himself continued his great theme of globalization. America's future is to spread democracy around the world. Never mind that since Bush and Obama, the trillions of American dollars spent to spread democracy around the world has not done a damn thing but strengthen more tyrants and America haters abroad, and destroyed the economy at home.
The theme of Bush's library is freedom. But…while he dreams of spreading freedom all over the world, in America, the citizens continue to lose theirs.
The Bush doctrine is that only in freedom will democracy flourish, while true and noble….it makes no sense to a religion based on anything BUT freedom. All the children of Islam are brainwashed before they start to walk. I guess Bush thinks sometime 3,000 years from now, the world's population of Muslims will decide to vote for someone who goes against everything they've learned from birth, and will thank George Bush for saving them from poverty and giving them democracy.
Frankly, I don't see it happening.
Nobody Thinks that the reason all these Presidents have to praise themselves on how what they personally did to further and help the world is because none of them have down a damn thing to help Americans. All of them are too busy helping the rest of the world. Globalization may make good speeches and wonderful ideas for American Presidents who build multi-billion dollar museums to themselves and keep their own families working forever, but they all just become another part of the 1 percent that live among the rich and famous. The Presidential museums will be the last remaining things standing if we keep on this "We will fix the world" path.
George W. Bush comes off as a very sweet and likable man, but so does Bill Clinton. But, they were paid to work for America…not to eliminate poverty all over the world because it makes them feel better about themselves. (Or helps out big corporations who want into the global markets.) We must not judge Presidents on how much we like them as people, but on what they have done for us. And let's be frank: None of them can be considered great.
So, when Bush started crying at the end of his speech— when he said the future of America will be great…a lot of us felt like crying with him. Everyone said he was crying because of all his friends. But to those watching, he was crying because America might never recover, and he knows it.
Personally, I don't think Bush is that dumb.
Instead of talking about what they have all done for America, all they could talk about is how much money they sent out to the rest of the world. It's enough to make you mad. And we still have good men dying in Afghanistan.
After the ceremony, I came across this video of Daniel Hannan, explaining how Margaret Thatcher took a dying England and brought it back to life. It's what we need here. A President that takes care of his country: FIRST.
That's what we should have heard today: George W. Bush could have brought America's strength back. Instead, he choose his own course of "saving the world."..and because he chose the wrong course, the communists, came forward.
George…you broke my heart.
You know, maybe our "Presidents" should just start building their libraries in Africa, and Iraq, and all over the world. And I apologize tonight to anyone who feels I'm too cynical about them all, because frankly my dear, I don't give a damn about any of them.
And I will spend the rest of my life, trying NOT to remember the years I spent feeling betrayed, by each and every one of them.
Nobody's Fool: Michele Backmann
Nobody's Fool
Last week, Michele Backmann mentioned Obama's lavish lifestyle, and here Bill O'Reilly ridicules her for it, and this Nobody Thinks he's wrong.
To take on Obama with such a "trivial" matter, when Presidents ALWAYS spend lots of money is not good, Bill says. She should concentrate on the important things.
BUT…in this economy, with so many people out of work, the President should be cutting back. His golf trip to play with Tiger Woods alone would have paid for so MANY things…tours at the White House for one. Our Presidents are big hypocrites and we should expect more from them.
Michele is right. But both of them missed the real point here. The President should have a budget. The office itself has grown into such an expensive KINGSHIP, they make any King or Queen on earth look like misers in comparison. For instance, what President had to build a 'replica' of the Oval Office while the real one was being upgraded? Most of them moved into a hotel, or another room.
The American people find it disgusting what Obama and Michelle are spending on our dime, taking monthly vacations…and Bill O'Reilly, seems to think it's okay for a President to go hog-wild with his money spending because all Presidents have done it.
That doesn't make it right. It's like saying "Hey mom…all the kids do crack!…It's Okay!" It's the same argument. Bill's argument is weak.
So, Michele Backmann wins the Nobody's Fool award for the week. I hope she hangs in there.


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Jon Hendricks
Jon Hendricks
Freddie Freeloader
Original Release Date: 1990
Track Listing
1. Jumpin' At the Woodside
2. In Summer
3. Freddie Freeloader
4. Stardust
5. Sugar
6. Take the "a" Train
7. Fas' Livin' Blues
8. High As a Mountain
9. Trinkle Tinkle
10. Swing That Music
11. The Finer Things In Life
12. Listen To Monk
13. Sing Sing Sing
Kevin Burke: Vocals, Baritone (Vocal)
Al Jarreau: Vocals
George Benson: Vocals
Tommy Flanagan: Piano
Larry Goldings: Piano
Al Grey: Trombone
Bobby McFerrin: Vocals
Rufus Reid: Bass
Jerome Richardson: Sax (Alto)
Janis Siegel: ?
Lew Soloff: Trumpet
Stanley Turrentine: Sax (Tenor)
Joe Temperley: Sax (Alto), Sax (Baritone), Sax (Tenor)
Cheryl Bentyne: ?
Jimmy Cobb: Drums
Britt Woodman: Trombone
Clifford Barbaro: Drums
Barry Finclair: Viola
Frank Foster: Arranger, Director, Sax (Tenor), Tenor (Vocal)
Geoff Gillette: Engineer
Josiah Gluck: Engineer
Tim Hauser: ?
Jon Hendricks: Leader, Sax (Tenor), Vocals, Producer, Alto (Vocals), Performer, Liner Notes, Vocal Arrangement
Judith Hendricks: Trumpet, Vocals, Soprano (Vocal)
Duffy Jackson: Drums
Howard Johnston: Engineer
Romero Lubambo: Guitar
Mark Lopeman: Conductor
Wynton Marsalis: Trumpet
Andy McCloud III: Bass
Tyler Mitchell: Bass
George Mraz: Bass
Alan Paul: ?
Al Rogers: Violin
Margaret Ross: Harp
Randy Sandke: Trumpet
Andy Stein: Violin
Stan Wallace: Engineer
Count Basie Orchestra: ?
Chaz Clifton: Engineer
Kiyomitsu Mihara: Design, Cover Design
David Berger: Arranger, Conductor, Transcription
Hiroyuki Hosaka: Mastering
Brian Lee: Engineer, Mixing
Josiah Cluck: Engineer
Takao Homma: Executive Producer
Ron McBee: Percussion
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Office Of Road Safety Western Australia: Tear
Tear along the dotted lines to see Electronic Stability Control at work.
ESC helps to correct your vehicle's direction after a sudden swerve, keeping you safely on the road. Ask for ESC in your new car. Visit rac.com.au for details.
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Dr. Jill Nagel Hester MD
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Dr. Jill Nagel Hester MD is a female Anesthesiologist, has 24 years of experience and practices in Anesthesiology and Family Medicine.
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With 7 out of 10 Latinos voting to re-elect Barack Obama, Republicans are debating whether to alter their hardline immigration stance, says Mark Engler.
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With 7 out of 10 Latinos voting to re-elect Barack Obama, Republicans are debating whether to alter their hardline immigration stance, says Mark Engler.
Filed in: Migration United States
The veteran journalist talks to Libby Powell about the dearth of tweeting Tuaregs, reporting war crimes and the scourge of guinea worm.
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Conservative Capitalism has won, time to say goodbye to the Age of Enlightenment says Anna Chen.
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Peru is said to be booming but the poor would never know it. Stephanie Boyd reports.
Inspiration, fears, and being drawn to Birmingham.
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Indian shortlisted Booker prize author speaks to Amy Hall about inspiration (overrated) and constructing an off-world station for gun-lovers.
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Zoe Leigh Smith reports on the tiny Caribbean island's strangling debt burden.
Wame Molefhe describes her joy as the first rains chase winter away.
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How can Egyptians make sure last year's uprisings have a lasting legacy? Maria Golia looks forward.
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With the presidential election looming, many Americans are finding their right to vote under assault says Mark Engler.
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A new leftwing café gives Steve Parry a break from empty consumerism and massive cups.
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PHOTO ESSAY: For Eritrean migrants, there is more dignity in death
The recent Saudi clampdown on migrant workers has brought campaigners onto the streets. Chris Matthews was with some of them in London.
Vanessa Baird ponders the tactics needed to resist austerity.
Jamie Kelsey-Fry reflects on the movement that has united people around the world.
Mari Marcel Thekaekara argues that we can all improve our wellbeing through traditional medicine and by slowing down.
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Sarasota - Terrace East condo sells for $575,000
May 13, 2010
A condominium in Terrace East tops all real-estate transactions from April 13 to April 16.
East County - Lakewood Ranch home sells for $925,000
May 12, 2010
Anthony and Susan Novellino, Lakewood Ranch, sold their home at 7107 Teal Creek Glen to James Nichols, Bradenton, for $925,000.
Longboat Key - Building Permits
May 12, 2010
These are the largest building permits issued by the Longboat Key Planning and Zoning Department for the week of April 30 through May 6, in order of dollar amounts. (GMD = Gulf of Mexico Drive)
Longboat Key - Key Real Estate: Sanctuary unit sells for $1.01 million
May 12, 2010
Sales dropped for the recording week of April 26 through April 30 to 11, down from the previous week's total of 24.
Longboat Key - Real Estate Reports
May 12, 2010
Michael Saunders & Co. signs on to Facebook platform; Jane Rees to be awarded with CIPS designation; Wagner Realty announces new Longboat Key agents
Longboat Key - Saunders signs on to new Facebook-based platform
May 6, 2010
The brokerage firm is the first in Florida to take advantage of the new service.
Sarasota - Harbor Acres home sells for $2.4 million
May 6, 2010
A home in Harbor Acres tops all real-estate transactions from April 5 to April 12.
East County - Rosedale home sells for $535,000
May 5, 2010
Arthur and Barbara Polk, Bradenton, sold their home at 4914 96th St. E. to James and Susie Lamy, Bradenton, for $535,000.
Longboat Key - Building Permits
May 5, 2010
These are the largest building permits issued by the Longboat Key Planning and Zoning Department for the week of April 23 through April 29, in order of dollar amounts. (GMD = Gulf of Mexico Drive)
Longboat Key - Lido Beach home sells for $1,895,000
May 5, 2010
The sale was the largest of 24 transactions — more than any other week to date in 2010 — recorded on Longboat, Lido, St. Armands and Bird keys for the week of April 19 through April 23 at the Sarasota and Manatee county courthouses.
- Dec
13 "Jazz at Two" with the Rodney Rojas Quartet
2:00 pm - 4:00 pm - Dec
13 Holidays around the Ranch
6:00 pm - 9:00 pm - Dec
13 Holidays Around the Ranch-Lakewood Ranch
6:00 pm - 9:00 pm - Dec
13 Cirque Des Voix "Joy and Wonder"
7:00 pm - 5:00 pm - Dec
14 FREE Pictures with Santa Cow!
8:00 am - 10:30 am - Dec
14 Free Spirograph Art Event For The Kids
10:00 am - 4:00 pm - Dec
14 Green Tara Empowerment
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14 Gingerbread Houses
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Country Club Shores home sells for $4.3 million
The sale was the fifth highest amount paid for a home on Longboat Key in 2013. -
Home in Sarasota by the Sea sells for $550,000
Ian Howard sold his home at 6630 Midnight Pass Road to Sunshine Homes of Sarasota LLC for $550,000. -
St. Armands Towers condo sells for $1.35 million
Gavriel and Catherine Salvendy sold their Unit 10-S condominium at 101 Benjamin Franklin Drive to Christopher and Susan John, of Norton, Mass., for $1.35 million.


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Feature Articles |
Rather than detecting the amount of an enzyme through traditional lateral-flow immunoassays, this technology detects only active enzymes and has the potential for detecting a plethora of enzymes and enzyme inhibitors involved in bond cleavage and bond formation.
The lateral-flow immunochromatographic assay (LFIA) format has achieved considerable commercial success, as demonstrated by strong sales of pregnancy and drugs-of-abuse tests.1-4 Because of its simplicity, low cost, and user-friendliness, LFIA is particularly attractive for point-of-care and over-the-counter tests. Although LFIA has been primarily used for qualitative detection, several new technologies capable of generating quantitative measurements have emerged.
Such technologies include colorimetry, conventional fluorescence, time-resolved fluorescence, phosphorescence, magnetic field measurements, surface-enhanced Raman, and up-converting luminescence.5-11 In fact, due to their proven robustness, some of these technologies have received regulatory approval for application in clinical diagnostics.
A lot of work has also focused on improving the detection of nucleic acids in LFIA. Several types of molecular probes and detection methods have been combined with lateral-flow assays to enhance the analysis of DNA and RNA.12 In addition, LFIA has been used for the simultaneous detection of several analytes. Although the throughput is limited, this detection platform can achieve multiplexing of analytes.13
LFIA does have limitations, such as the ability to determine only the amount of antigens and haptens in a sample. For detecting enzymes, LFIA cannot differentiate between active enzymes and denatured forms. In some cases, such differentiation is desirable and essential for clinical diagnosis. For example, active proteases from pathogens such as hyphal yeasts are believed to be responsible for vaginal yeast infections.14,15 Monitoring the enzymatic activity of proteases would also benefit the monitoring of a drug therapy's efficacy.
Although tremendous efforts have been made in developing and commercializing LFIA for detecting a large number of analytes, minimal work has been done in adapting the lateral-flow assay format for detecting enzyme activity. This article discusses efforts to integrate an enzyme reaction with a lateral-flow assay technology. In addition to detecting enzyme activity, these assays also provide a simple method to screen and identify enzyme inhibitors. Compared with conventional enzyme assays, lateral-flow enzyme assays require no instrumentation. Finally, these assays are simple, rapid, and inexpensive, yet provide high sensitivity.
Methods and Materials
Surface-functionalized particles and magnetic particles, including carboxylated and streptavidin-modified blue particles (SA-BP, 0.3 µm), were obtained from Bangs Laboratories. Sulfo-NHS-LC-biotin was acquired from Pierce Biotechnologies. Bovine serum albumin (BSA), β-casein, streptavidin, anti-biotin antibody, and Streptomyces griseus (SG) protease were bought from Sigma-Aldrich. Phosphate buffered saline (PBS; 50 mM sodium phosphate, 100 mM sodium chloride, pH=7.2), sodium borate, carbodimide, and ethanolamine were purchased from Polysciences Inc. Nitrocellulose membranes, supporting cards, cellulose samples, and wicking pads were obtained from Millipore Inc.
Preparation of Casein-MP. 25 mg of 0.35 µm magnetic particles (MP) with carboxylic acid surface groups were washed once with borate buffer and once with PBS. The separation of the magnetic particles from the supernatant was accomplished by a magnet separator. The washed particles were suspended in 1 ml PBS buffer, and 28.8 mg carbodimide in 1 ml PBS buffer was added. The mixture was gently shaken for 15 minutes. The particles were washed four times with a borate buffer (pH=8.5, 0.1 M).
The washed particles were suspended in 1 ml borate buffer, and two mg β-casein in 1 ml borate buffer was added. The resulting mixture was gently shaken at room temperature overnight. The particles were washed three times with water, and suspended with one ml of 50 mM ethanolamine solution in water. The mixture was shaken for 30 minutes. The particles were washed five times with water and suspended in 2 ml borate buffer. These particles will be referred to as casein-MP for the remainder of this article.
Preparation of Casein-BP. The procedure is the same as covalent attachment of β-casein to magnetic particles, except that carboxylated blue latex particles (0.3 µm) were used to replace the carboxylated MP. Separation and washing were accomplished by centrifugation. These particles will be referred to as casein-BP for the remainder of this article.
Preparation of Biotin-Casein-MP and Biotin-Casein-BP. Four mg of casein-MP, or casein-BP, in 300 µl borate buffer was added to 1 mg sulfo-NHS-LC-biotin in 200 µl borate buffer (50 mM, pH=8.4). The mixture was gently shaken for four hours and washed five times using tris buffer (20 mM, pH=7.2). The particles were suspended in 1 ml tris buffer for storage and were designated as biotin-casein-MP or biotin-casein-BP.
Preparation of Biotin-BSA. 500 mg BSA in 9 ml borate buffer was added to 300 mg sulfo-NHS-LC-biotin in 1 ml borate buffer (50 mM, pH=8.4). The mixture was allowed to react for three hours at room temperature. The mixture was dialyzed five times in PBS buffer. The biotinylated BSA is designated as biotin-BSA.
Preparation of Type I Strips. A hi-flow 120 nitrocellulose membrane on a plastic support card was striped with a line of 5 mg/ml biotin-BSA in water to form Capture Zone I. A similar line of polylysine solution (50 mg/ml) in water was printed to form Capture Zone II. All striping was conducted using a dispenser by Kinematic Automation. The printed card was dried overnight at 37° C.
A cellulose wicking pad was laminated to the end of the nitrocellulose membrane, close to Capture Zone II, with a 5-mm overlap. A conjugated pad and a sample application pad were laminated with the side of the nitrocellulose membrane, close to Capture Zone I. For the conjugate pad, a 10-cm-long strip of glass fiber pad material was loaded with a solution of 50 µl of 1% SA-BP, 200 µl 20% sucrose, 100 µl 2% tween 20, and 250 µl 20 mM tris buffer (pH=7.2), followed by drying at 37° C for four hours. The card was cut into 4-mm-wide strips.
Preparation of Type II Strips. A hi-flow 120 nitrocellulose membrane on a plastic support card was striped with a line of 2 mg/ml anti-biotin antibody in water to form Capture Zone I and with a line of polylysine solution (50 mg/ml) in water to form Capture Zone II. The membrane was dried overnight at 37° C. A cellulose wicking pad was laminated to the end of the nitrocellulose membrane, close to Capture Zone II, with a 5-mm overlap. The card was cut into 4-mm wide strips.
Detection of Protease Using Magnetic Substrate Conjugates. Each of the six samples containing 80 µg of biotin-casein-MP in 60 µl 20 mM tris buffer (pH=7.2) was spiked with a different amount of metalloprotease, ranging from 0.02 to 4.0 µg per sample. A control sample containing 200 µg of deactivated metalloprotease was included. The samples were incubated at room temperature for 20 minutes, and the magnetic particles were removed by a magnet separator. 20 µl of the resulting supernatant from each sample was applied to the sample pad of each type I device. The strips were developed for 30 minutes (see Figure 3).
Detection of Metalloprotease Using Non-Magnetic Substrate Conjugates. Five samples, containing 0.3 µg of biotin-casein-BP in 40 µl 20 mM tris buffer (pH=7.2), 1% tween 20, were spiked with 0-40 ng of metalloprotease. The samples were incubated for 20 minutes at room temperature. A type II strip was inserted into each sample for development. The strip was developed for 30 minutes (see Figure 5).
Figure 1. Schematic representation of lateral-flow enzyme assays. S and P represent substrate and product, respectively. LFIA stands for lateral-flow immunoassay. |
Results and Discussion
The general process for lateral-flow enzyme assays is depicted in Figure 1. The method combines an enzyme reaction with LFIA. An enzyme substrate is catalyzed by an enzyme to generate one or more products. The reaction may cleave a chemical bond, form a new bond, or result in a conformational isomerization. If the test is intended to screen for an enzyme inhibitor, the inhibitor will also be present in the samples. The lateral-flow test strip can be used after the enzyme reaction is completed, or the enzyme reaction can be integrated into the lateral-flow test strip, which will need a certain mechanism to control reaction time and condition. For example, a lateral-flow test strip may be coupled with a microfluidic device to control reaction time and conditions.
Theoretically, LFIA can be constructed to detect the disappearance of a substrate, the appearance of products, or both simultaneously, provided antibodies are available. However, such antibodies may not exist, especially for small molecular substrates such as a short peptide. In these cases, it is desirable to tag the substrate with specific binders and/or probes. Depending on the substrates and enzymes, different assay formats can be constructed.
Figure 2. Process of a lateral-flow enzyme assay using magnetic particles. |
Magnetic Lateral-Flow Enzyme Assays
Figure 2 depicts an assay format that uses a magnetic particle, a substrate, and a specific binder I (B1) for detecting an enzyme, which involves a bond cleavage to release a product. An enzyme substrate is covalently linked to a magnetic particle and B1 to form a magnetic particle-based substrate conjugate. When an enzyme cleaves the substrate conjugate to release B1 from the magnetic particle, the free B1 can be separated from both digested and intact substrate conjugates by a magnetic field, and detected by a lateral-flow test strip.
To demonstrate the principle, metalloprotease from SG was used as a model system. β-casein is a good substrate for the metalloprotease. Biotin-casein-MP was prepared as an enzyme substrate.
The amide bonds of β-casein on biotin-casein-MP are cleaved by the protease to release some or all of the biotin moieties from the MPs. The MPs are removed by a magnet, and the biotin molecules in the solution can be detected on type I lateral-flow test strips. The lateral-flow test strip for detecting biotin can be universally used for assaying various enzymes as long as the enzyme reaction releases biotin from a substrate conjugate.
Figure 3. Detection of protease by a lateral-flow enzyme assay using magnetic particles. |
Figure 3 shows the results for detecting metalloprotease in a series of samples spiked with a different amount of metalloprotease. Without active protease, all biotin molecules are attached to the MPs and are removed from the solution by a magnet, and therefore no biotin remains in the solution. When the solution is applied on a type I lateral-flow test strip, all the SA-BP particles released from the conjugate pad were captured in Capture Zone I by the immobilized biotin-BSA to show a strong blue line (200 µg deactivated). Capture zone II showed almost no blue signal.
In the presence of 0.02-0.2 µg of active protease, some of the biotin molecules were released from the MPs. The released biotin molecules competed for the SA-BP so that fewer SA-BP were able to be captured by biotin-BSA in Capture Zone I, resulting in a weaker signal. SA-BPs saturated with released biotin molecules passed through Capture Zone I and bound to the polylysine in Capture Zone II to produce a strong signal. The positively charged polylysine in Capture Zone II can capture any particles with a negative charge. Therefore, the color density of Capture Zone I decreased as the enzyme activity increased, while the color density of Capture Zone II increased. When the amount of the protease was one or four µg, all the biotin molecules were released from the substrate conjugates into the solution. In this case, Capture Zone I showed no blue line, while Capture Zone II formed a strong blue line.
Figure 4. Process of a lateral-flow enzyme assay using colored particles |
Particle Lateral-Flow Enzyme Assays
Figure 4 depicts another lateral-flow enzyme assay format that uses a non-MP such as a colored or fluorescent particle. An enzyme substrate is covalently tagged with a specific binder I (B1) and a colored particle to make a substrate conjugate. Without an enzyme, the particles are captured by a second binder (B2, specific for B1) that is immobilized in Capture Zone I on a type II lateral-flow device to show a strong color signal. Capture Zone II should show either no signal or a weak signal if the capturing capacity of Capture Zone I for the substrate conjugate is more than the total amount of the substrate conjugate.
In the presence of an enzyme that cleaves the substrate, some or all of the B1 will be released from the particles. The released B1 can flow faster than the substrate conjugates to bind with the B2 in Capture Zone I. Furthermore, the digested or partially digested substrate conjugates will have fewer B1 moieties; therefore, they will have less or no chance of being captured by the B2 in Capture Zone I to produce a weaker signal or no signal. The digested or partially digested substrate conjugates pass through Capture Zone I and are captured by the polylysine in Capture Zone II to show a stronger signal.
Figure 5. Detection of protease by a lateral-flow enzyme assay using colored particles |
To demonstrate the principle of the assay format in Figure 4, biotin-casein-BP was prepared as a substrate conjugate for metalloprotease from SG. Figure 5 shows the developed test strips for analyzing samples spiked with 0-40 ng of metalloprotease. In the absence of metalloprotease, the substrate conjugates are captured in Capture Zone I of a type I strip to show a strong signal. Capture Zone II showed no signal because all the substrate conjugates were captured in Capture Zone I.
In the presence of 0.5-40 ng of metalloprotease, the enzyme digested the substrate conjugates to release some of the biotin molecules so that the substrate conjugates have fewer or no biotin moieties. The released biotin molecules and the biotin moieties still attached to the substrate conjugates competed for the anti-biotin antibody in Capture Zone I to produce a weak blue signal, while some of the digested or partially digested substrate conjugates passed through Capture Zone I and were bound at Capture Zone II to produce a stronger blue signal.
When 40 ng of metalloprotease was used, all the biotin moieties were released from the substrate conjugates, and no more biotin was attached to the blue particles. The color intensity in Capture Zone I was very weak, and the blue color intensity in Capture Zone II was strong. In general, the color intensity of Capture Zone I decreased, and the color intensity of Capture Zone II increased as the amount of protease in the sample increased.
Figure 6. Dose response curve for metalloprotease detection (Y axis: proportional reflectance, I2/ (I1+I2)). |
The color intensities of Capture Zones I and II can provide semi-quantitative or even quantitative measurements of the amount of active enzyme. The reflectance intensities of both Capture Zones I and II were measured using an in-house-developed scanner. Figure 6 shows the reflectance of Capture Zone II (I2) over the sum of Capture Zones I and II (I1 + I2) as a function of the amount of metalloprotease in a sample. The dose response curve shows a good linear correlation between the reflectance intensities and the amount of protease prior to saturation.
This article has demonstrated the adaptation of the lateral-flow format for detecting active enzymes. Lateral-flow enzyme assay techniques retain all the benefits of LFIA such as simplicity, user-friendliness, and low cost. Although this current study was focused on detecting the enzymes involving bond cleavage, the assay techniques could be adapted to enzymes involved in bond formation and conformational isomerization. In addition to detecting enzymes, the assay techniques should also be useful for detecting any molecular species involved in an enzyme reaction, such as an enzyme inhibitor. The technologies should find a wide variety of real-world applications such as POC and OTC tests for vaginal yeast infections (using spartyl proteases as biomarkers) and chronic wounds (using proteases from bacterial pathogens as biomarkers).
1. G Posthuma-Trumpie, J Korf, and A Amerongen, "A Dual Gold Nanoparticle Conjugate-Based Lateral-Flow Assay (LFA) Method for the Analysis of Troponin I," Analytical and Bioanalytical Chemisty 393 (2009): 569-582.
2. D Brooks, et al., "A Rapid, Quantitative Whole Blood Immunochromatographic Platform for Point-of-Care Testing," Clinical Chemistry, 45(1999): 1676-1678.
3. RG Sommer, "Quantitative Detection of Analytes on Immunochromatographic Strips," U.S. Patent 5,569,608 (1996).
4. H Kuo and LA Meritt, "Method for the Determination of Analyte Concentration in a Lateral-Flow Sandwich Immunoassay Exhibiting High-Dose Hook Effect," U.S. Patent 6183972B1.
5. CR Thornton, AC Groenhof, and R Forrest, "A One-Step, Immunochromatographic Lateral-Flow Device Specific to Rhizoctoniasolani and Certain Related Species, and Its Use to Detect and Quantify R. Solani in Soil," Phytopathology 94 (2004): 280.
6. JL Lambert and AM Fisher, "Diagnostic Assays Including Multiplexed Lateral-Flow Immunoassays with Quantum Dots," U.S. Patent Application 20050250141A1 (2005).
7. X Song, "Lateral-Flow Assays Using Time-Resolved Luminescence," IVD Technology 15, no. 4 (2009): 31-37.
8. X Song, "Membrane-Based Lateral-Flow Assay Devices that Utilize Phosphorescent Detection," U.S. Patent Application 20050112703A1 (2005).
9. C Feistel, "Systems and Methods for Performing Magnetic Chromatography Assays," U.S. Patent 6136549 (2000).
10. MC Burrell, et al., "Raman-Active Lateral-Flow Device and Methods of Detection," U.S. Patent Application 20070059203 (2007).
11. P Corstjens, et al., "Use of Up-Converting Phosphor Reporters in Lateral-Flow Assays to Detect Specific Nucleic Acid Sequences: a Rapid, Sensitive DNA Test to Identify Human Papillomavirus Type 16 Infection," Clinical Chemistry 47 (2001): 1885-1893.
12. X Mao, et al., "Disposable Nucleic Acid Biosensors Based on Gold Nanoparticle Probes and Lateral-Flow Strip," Analytical Chemistry 81 (2009): 1660–1668.
13. Y Oku , et al., "Development of Oligonucleotide Lateral-Flow Immunoassay for Multiparameter Detection," Journal of Immunological Methods 258 (2001): 73-84.
14. J. Naglik, et al., "Candida Albicans Secreted Aspartyl Proteinases in Virulence and Pathogenesis," Microbiology 154 (2008) 3266-3280.
15. J Naglik, S Challacombe, and B Hube, "The Secreted Aspartyl Proteinases Sap1 and Sap2 Cause Tissue Damage in an In Vitro Model of Vaginal Candidias is Based on Reconstituted Human Vaginal Epithelium," Microbiology and Molecular Biology Reviews 67 (2003): 400-428.
Xuedong Song is a technical leader in corporate research and engineering at Kimberly Clark Worldwide (Dallas). He can be reached at email@example.com.