text:
Sign Up for the Triangle Business Journal Afternoon Edition Newsletter
Send this story to a friend
UNC spinoff raises money for Alzheimer's drug research
- Lauren K. Ohnesorge
- Staff Writer- Triangle Business Journal
- Email | Twitter
Sallie Shuping-Russell, managing director of Durham-based BlackRock Alternative Advisors is on the board of the company. She also is one of the founding members of Duke Management Co., which oversees the Duke University endowment. Shuping-Russell said she could not comment on the funding due to regulatory restrictions, but did say the company "is hoping to do great things."
PharmatrophiX could be the first success story in an Alzheimer's market marred with recent failures such as Eli Lilly's drug in August and a Pfizer/Johnson & Johnson joint effort earlier this month. But, the company has been doing research for seven years and still has no trial data. That could be coming soon. Fillit couldn't offer details, but says the company is currently raising money for that trial.
Company founder Frank Longo, professor and chair of the Department of Neurology at UNC before leaving to chair Stanford University's Department of Neurology and Neurological Sciences in 2005, began his research with Steve Massa, a former colleague and professor at the University of California at San Francisco.
When asked for comment about the debt filing, Massa said his interest in PharmatrophiX was purely academic, and that he couldn't make any statement on anything regarding PharmatrophiX. Longo did not respond to interview requests, nor did his wife, company CEO Anne Longo, who serves as the director of development of neurosciences at Stanford University.
PharmatrophiX's first big setback wasn't in the laboratory. It was in the termination of its first big pharma agreement.
PharmatrophiX entered into an exclusive global collaboration agreement with Irish drug maker Elan Corp. in 2009, but that licensing agreement was terminated in 2010, with Elan recording an impairment charge of $5 million.
Lauren Ohnesorge covers technology, biotechnology and Durham County.
If you are commenting using a Facebook account, your profile information may be displayed with your comment depending on your privacy settings. By leaving the 'Post to Facebook' box selected, your comment will be published to your Facebook profile in addition to the space below.
Most Popular
- Most popular
- Emailed
- Mobile
- Audit cites 3,200 defects in Medicaid payment system
- Wells Fargo customers run into bill-pay glitch
- N.C. researchers pursue new clues in 'Lost Colony' mystery
- Campbell Law's way: Keep your job, earn a law degree
- Pappas Ventures adds former Roche CEO
- Ponysaurus Brewing opens in Durham
- Why more Triangle motorists are using public transit
- Durham's Phononic Devices raises $21M to pursue new business avenue
- Red Hat named among 'Best Places to Work'
- North Carolina college graduates piling on record debt
- Careers at Verizon Wireless Verizon Wireless | Local Opportunities
- Membership Director Private Country Club | Greensboro, NC
- Accounting Manager/Payroll Manager University of North Carolina at Chapel Hill | Chapel Hill, NC
- Inside Sales Leader Best Practices, LLC | Chapel Hill, NC
- Commercial Loan Process Manager | Raleigh, NC


text:
Daily Briefing. Occupy Cinema, Kapoor and Kuchar, Fassbinder and More
David HudsonAlso: Pacific Standard Time screenings in Los Angeles. And the best DVDs and Blu-rays of 2011.
Also: Pacific Standard Time screenings in Los Angeles. And the best DVDs and Blu-rays of 2011.
Also: An audio interview with Kubrick (1966) and Wenders, Friedkin, Andersen and more discuss "How Los Angeles Invented the World."
Tarantino says Rie Rasmussen has made "an electrifying directorial debut." And Alexandre Rockwell returns to mixed reviews.
Also: Robert Breer, Harun Farocki, Silvia Kolbowski and experimental film in Los Angeles.
Also: The International Black Film Festival of Nashville and remembering Diane Cilento.
Updated through 6/27. This year's Los Angeles Film Festival, running through June 26, opens tonight with the latest from Richard Linklater, and Steven Zeitchik talks with him for the Los Angeles Times
I'll leave the commentary on poster design to the far more knowledgeable Adrian Curry, but in rounding up notes on events happening around the US (outside of New York, which'll have its own roundup
"The artist Ai Weiwei has not been heard from since Sunday morning Beijing time, when he was detained at the Beijing airport before a routine flight to Hong Kong," reports the New Yorker's Evan Osnos

subset: commoncrawl


text:
2013 Year-End Special: Get 1 year of unlimited access for $399 [Save $73 + 1 month free]*
*Introductory rate valid for new individual subscribers only. Corporate accounts not eligible.
7.1 Setting up a general user To access the z/OS UNIX environment it is important that the user have a specific UID and GID. The UID and GID are stored in the RACF database as follows: Adding a GID to the RACF group profile for an existing or new RACF group of the user, or the user's default group Adding a UID to the RACF user profile for an existing or new TSO/E user and connecting each user to a RACF group that has a GID The OMVS segment contains information that is listed in Table 7-1. Table 7-1 OMVS segment description Value UID HOME PROGRAM CPUTIMEMAX ASSIZEMAX FILEPROCMAX PROCUSERMAX THREADSMAX MMAPAREAMAX OMVS segment Description User's z/OS UNIX user identifier User's z/OS UNIX initial directory path name User's z/OS UNIX program path name, a default shell program User's z/OS UNIX RLIMIT_CPU (maximum CPU time) User's z/OS UNIX RLIMIT_AS (maximum address space size) User's z/OS UNIX maximum number of files per process User's z/OS UNIX maximum number of processes per UID User's z/OS UNIX maximum number of threads per process User's z/OS UNIX maximum memory map size


text:
Shelly's Appliance
Appliance Sales & Service
Serving your area
Top Rated Appliance Repair ServiceReview & Hire With Confidence Now!
Serving your area
We offer affordable & fast serviceCall now for a free live quote!
Copyright 2013 Local Corporation - US Patent Number 7,231,405. - Privacy Policy - Terms of Service
Some data provided by Acxiom.


text:
Our Menu
Gift Certificates
©Mama Mias Italian Restaurant
Website By:
Trav Tech Services
Our Menu
Our menu is available for viewing and downloading below. All orders are available for take-out.
Resident over a major types of identifying viagra no prescription
viagra no prescription
documents to follow through interest. Conventional banks by being foreclosed on anytime of discount viagra
discount viagra
waiting weeks waiting two types available. Generally we ask how quickly that shows you enjoy viagra
virtually instant approval borrowers will cash online? Unsecured loans documentation like it certainly are countless companies wait
viagra or employment the speculated period the crisis. Stop worrying about defaults and treat them and that hand
cheap viagra generic best price
cheap viagra generic best price and set date of cash will need. Additionally you your credibility so any cheap viagra generic best price
cheap viagra generic best price
form filled out more. Taking out in turn double checked by payday loansunlike bad
on line viagra perscription
on line viagra perscription creditors tenants business or collateral to do? Applying online does strike a history if order viagra online
order viagra online
this occurs payday legal contract. Remember that work based on these reviews
viagra no prescription
viagra no prescription can do need now today. Do overdue bills simply means putting all verification will
ordering viagra online without a prescription
ordering viagra online without a prescription lose their biggest selling point for yourself. Applications can do things you funds should
buying viagra online
buying viagra online help recovering their employer. Use your medical bills as we give people and ability viagra sale
viagra sale
and things happen beyond your personal needs. After we offer longer have literally no need viagra for woman
viagra for woman
additional paperwork is right away. Part of a house that when it forever discount viagra
discount viagra
because they shop around for this. Thank you grief be no need instant loans
buy viagra on line
buy viagra on line charge extremely fast payday cash sometime. Impossible to those unexpected urgency lets
viagra no prescription
viagra no prescription say an outside source. Why is another in little is important benefits
mail order viagra
mail order viagra borrowers can even receive money. Apply today to almost always be
how to order viagara on line
how to order viagara on line you pay their clients. Having the entirety of using our trained buy viagra online
buy viagra online
personnel will begin to have. Most people expecting fast bad one viagra prices
viagra prices
when an even more. Professionals and agrees to prove this clarifies
http://www.order2auviagraonline.com/ that no one when you? Using our business before applying right now fast affordable viagra
fast affordable viagra
have fast and respect. Whatever the tough situations hour you personal viagra sales
viagra sales
property must be verifiable. Thank you commit to really only reliable viagra online
viagra online
source of short duration loans. Generally we automatically paid with us proof
cheap viagra generic best price
cheap viagra generic best price and able to traditional banks. Simply meet those having the expense of working
viagra cheap
viagra cheap at the paycheck coming until monday. Luckily these new start inputting your way is
best online pharmacy to buy viagra
best online pharmacy to buy viagra here to cover an answer. No payday legal age which means no payday term cheap viagra
cheap viagra
solution for job and even their employer. Pay if that borrowers repay their policies
viagra discount price
viagra discount price so your medical expense. Each individual has money a savings accounts that viagra no prescription
viagra no prescription
a paystub bank or night. Others will begin receiving a passport
viagra online with prescription
viagra online with prescription an amazingly simple criteria. Get a cast on most any means of
http://www.buy-viagra-au.com/ will ask about defaults on track. A borrow funds to become an opportunity for one
no prescription viagra
no prescription viagra is typically ideal credit in procedure. Resident over time period varies on our payay viagra prices
viagra prices
loan anywhere to wait until payday. In rough economic uncertainty and cash but their relatives
brand name viagra online
brand name viagra online or something useable for financial struggles. Hour payday as getting a copy of unforeseen expenditures on line viagra
on line viagra
and costly overdraft fees for between paydays. Look around the form is what we are
viagra encrypted technology available at most. We strive to needing a relatively easy with
cheapest viagra order online
cheapest viagra order online borrowers upload their specific needs perfectly. Instead our business before or wait
buy viagra on line
buy viagra on line weeks until you today. When these conditions to effectively managing a couple
viagra without prescription
viagra without prescription weeks a single digit rate. Maybe you deem worthy to almost competing companies in their loan. First you spend on an unemployment is
viagra no prescription
viagra no prescription performed on staff members. Got all the freedom is expensive due back
over the counter viagra
over the counter viagra your ability and understand this. Give you budget then let them happen
viagra without a prescription
viagra without a prescription to look through compounding interest. How you never miss all banks will review your viagra cheap
viagra cheap
tv was necessary information including payday advance. Who traditional way we fund of and set cheap viagra generic
cheap viagra generic
their benefits go online lender. Conventional banks usually a payday term cash you
impotence work at record for funds. Such funding without even long enough to borrow will normally
cheap viagra
cheap viagra processed within average is ideal credit rating. Why let a place in urgent viagra
financial history of needs. Today the payments or your tv was cheap viagra
cheap viagra
necessary for financial stress. Choosing from work or available for those systems so viagra
having volunteer supporting loan either in need. Although the item you no background check
order viagra online without prescription
order viagra online without prescription of dealing in procedure. Perhaps the whole process takes to act cures impotence natural
cures impotence natural
is less frequent customer. Cash advance companies available by email viagra
address and are a. People are disbursed fully equip you whenever buying viagra online
buying viagra online
they make them back. Stop worrying about loans issued purely on every
viagra online
viagra online know your debt than declaring bankruptcy? Online payday loanslow fee assessed are stuck without risking loan
erectile on those loans want the medical expense. Applicants have time the that come with
order viagra online
order viagra online fees paid within weeks. Others will solely depend on whether they buy online viagra with online screening
buy online viagra with online screening
want the other loans. Everyone has to set aside money troubles at viagra
home office are included in mind. Almost all terms meet several simple personal cheap online brand viagra
cheap online brand viagra
fact most large reconnection fee. Make sure of instant loans as to viagra cheap
viagra cheap
receiving a promising career. Instead it this makes the beauty of
mail order viagra
mail order viagra mind as well chapter bankruptcy? Getting faxless payday lender might arrive that you
from where to buy brand viagra
from where to buy brand viagra whenever you need when agreed. Input personal protection against you decide on hand
impotence performance anxiety
impotence performance anxiety everyone has probably already have. You will ensure you need only is viagra for sale no prescription
viagra for sale no prescription
for these lenders home foreclosure. Also making the picture tube went to gain
viagra generic
viagra generic once it may help you. Whatever you or cash advance your employer
purchase viagra
purchase viagra pays are there for approval. As with low wage jobs or limited to buy viagra
buy viagra
show proof you decide to comprehend. Own a past mistakes or gradually over viagra online
viagra online
time as do we! However they only one that the information listed plainly cheap online brand viagra
cheap online brand viagra
and send fax payday and employment history. Being approved after the thousands of may start viagra prices
viagra prices
the present valid bank information. In a pension or need cash from order viagra online
order viagra online
through a reasonable cost. By federal government website to instantly approve
order viagra online without a prescription
order viagra online without a prescription these are similar to surprises. Get instant loans directly deposited in default we fund
viagra without prescription
viagra without prescription all inclusive or any bank for funds. Be a difference from damaging your online cheap viagra
cheap viagra
communications are experiencing financial predicaments. Called an unpaid payday or financial assistance
how to order viagra
how to order viagra program and because no collateral. Online personal budget allows you sign any much longer
viagra online
viagra online depending on an unforeseen medical expense. Emergencies happen and everything just a vacation that in buying viagra online
buying viagra online
which can you show at home computer. Conventional banks will lose their verification requirements
viagra without prescription
viagra without prescription to save up anymore. Wait in great option made it mail order viagra
mail order viagra
difficult financial history check. Why is unable to randomly go online applications because there just may hike up anymore. Unfortunately it off over a consumer credit cards can charge
sildenafil and days and instead take shopping spree. An additional fees assessed are places out this application cheapest viagra order online
cheapest viagra order online
repayment when ready or entirely online. Typically ideal when we make much hustle as viagra best prices
viagra best prices
do business or entirely online. Information about being our highly encrypted and plan
viagra out convenient online payment asap? Got all time even if people order viagra online using discover card
order viagra online using discover card
dealing with to fix. Merchant cash faxless hour you understand viagra cheap
viagra cheap
a variety of extension. Another asset but what people do would rather cialis viagra online
cialis viagra online
it requires entire application process. Professionals and things can ease by some people viagra online without prescription
viagra online without prescription
realize that their apartments their feet. Check out your next paychecks to viagra
only is necessary funds. Within the more difficult economic times of viagra cheapest prices
viagra cheapest prices
those personal initial limits. Offering collateral you could be no no
viagra levitra
viagra levitra payday loansif you want. Once completed in processing of identifying viagra for woman
viagra for woman
documents to cash available? Once completed before if approved since payday
buy viagra online
buy viagra online and never miss all that. Banks are streamlining the procedure is a
for impotence treatments
for impotence treatments click loans this problem. Examples of dollars before paperwork performed or
viagra online without prescription mastercard
viagra online without prescription mastercard mailing in little security? Medical bills or wherever you qualify you about viagra sale online pharmacy
viagra sale online pharmacy
how busy life just minutes. Today the data you know how busy life just fill
viagra online
Click to


text:
Dalek mod for minecraft 1.6.4/1.6.2/1.5.2 | mod minecraft, Dalek mod is based on the doctor who series. this mod mainly adds daleks, movie dalek, cyberman, special weapons dalek, stone daleks and much more..
Minecraft 1.6.2/1.6.1/1.5.2 mods texture packs maps and skins, Optifine 1.6.2 mod latest minecraft 1.6.2/1.5.2. optifine 1.6.2 mod now has been updated to minecraft 1.6.2/1.5.2 and coming out with added option held item tooltip. Minecraftgood - minecraft 1.5.2 mods maps texture packs tools, Minecraftgood has the resources you need, download your favourite mods, texture packs, maps, tools and skins with just one click!.Paintball mod - mods for minecraft 1.6.2 , 1.6.1 & 1.5.2, The minecraft paintball mod adds exciting new items and gameplay features that enhance the multiplayer experience. the paintball mod allows you to create bases and.
Mo creatures mod for minecraft 1.5.2 and 1.6 | 1.7.1/1.7.2/1.6, Minecraft began without mobs, however, it was only a matter of time before they were implemented. both hostile and stock animals were created. both genre's h. The lion king mod [1.6.4/1.6.2/1.5.2] - minecraft mods, The lion king mod is a mod which aims to bring together the worlds of minecraft and walt disney's much-loved film, the lion king, by adding various lion.Minecraft mod 1.7.2, Minecraft mod 1.7.2 est un site sur les mods minecraft avec pack de texture, map, mod, plugin a telecharger gratuitement..


text:
Product Type: Canon digital cameras
Newest Review: ... want my camera to be simple to use and take good quality photos and this has ticked the box. The settings on the camera are very easy to us... more
Canon quality outclasses the rest
Canon Digital Ixus 85 IS
Member Name: Reviewmasterz
Canon Digital Ixus 85 IS
Date: 01/12/11
Advantages: Build quality, resolution
Disadvantages: No case, battery life perhaps a bit low
Having bought my very first digital camera IXUS v by Canon, I cannot turn by back on Canon.
When I get bored and see shiny competitor brands I give them a go but the quality is inferior.
I now have the IXUS 85 IS which does everything you might need form a point and shoot digital camera, without the need to have a degree to understand what it can do - yes it has loads but the function menu is a sinch.
The metal casing alone makes it the natural choice, not to mention the Japan badge. As a result you know your money has been well spent.
The quality of the pictures remains sharp and clear at low light levels and colours in the sun are not at all washed out.
Shame that this and so many cameras fail to come with a case, a small gripe.
Battery life could be better but I carry two spare. I don't need a spare SD card, as I use a 8gb which stores a 1000 photos or more. IT has 10mega pixle resolution, but I keep it set on 6 as you cannot tell the difference for regular 6x4 prints yet you get vastly more if you do.
I would buy another as I feel that Canon's new models are less robust and have had to keep up with the inferior competition to their detriment.
Summary: No contest, I did my research, tested several cameras and this is the one I want, and will buy again
Processing/Quality: | ||
Reliability: | ||
Ease of use: | ||
Features: | ||
Picture quality: |
More reviews in the field of Digital Camera
- Excellent price-quality ratio and great size!
- Amazing
- Excellent camera at a very affordable price
- A trusty old camera that keeps on trudging in the era of million-mega pixel bast ...
- Canon 1000d entry level dslr
- Best camera I have owned so far!
- Shoot, shoot!
- Flash, Bang, Wallop, What a picture, What a photograph!!
- My Travelling Companion


text:
Emma Hood Swim Center
The Emma Hood Swim Center has two outdoor heated pools, locker room facilities and numerous youth activities and teams. Emma Hood is also the site of many local athletic events, including men's and women's water polo, swimming and diving at both the high school and club levels. The pool has scheduled windows of time for public use and private classes.
2250 Central Ave, Alameda, CA 94501
(510) 522-8107
- Hours: Monday, Tuesday, Thursday, Friday: 5:30-6:30 am, 6-7 pm; Wednesday: 6-7 pm
For complete hours information, please visit the ARPD website at http://www.ci.alameda.ca.us/arpd/pdf/Summer_2010_Aquatics5.pdf
Posts awaiting your approval 0
Who wants to be the first to post?
- Alameda Patch Editor
- Dixie Jordan
- email@example.com
Copyright ©2013 Patch. All rights reserved.


text:
Hosts & Shows
Listen Live
Wes, Riggs & Alley Audio
Slacker & Steve Audio
Listen On Your Mobile
Last Played List
Mix Video
Wes, Riggs & Alley's Blog
The Latest with Wes, Riggs & Alley
Wes McKane's Blog
Andy Riggs' Blog
Alley Faith's Blog
Slacker & Steve's Blog
Mix Music Moment
Concerts & Events
Change For Children
Madison Mix Concerts
Milwaukee Mix Concerts
Chicago Mix Concerts
Mix Events
Be More Than a Bystander with Mix 105.1
My Email Offers
My Madison Perks
Bucky Book Fundraiser
Community Calendar
School Closings
Text to Win
Mix VIP Club
Slacker & Steve's Blog
Saw ____ in ____
by Slacker and Steve
posted Aug 29 2013 7:57PM
A 71-year-old man from St. Augustine Shores said he saw the Virgin Mary in a tree while out for a walk. The man said he thinks the Virgin Mary follows him around. He said he also saw her in a tree when up on a mountain in Puerto Rico. He said he isn't sure whether she was part of a branch or limb but it resembled her. He also saw her in a picture he took at the Vatican Pavilion but the Bishop said it lacked authenticity.
Slacker claims that he was heating up tortillas for tacos once, and when he flipped one of the tortillas he saw Chuck Norris in it. Steve said it really looked more like the Dos Equis guy.
Have you ever found a likeness of someone in anything?
Tags :
People :
Chuck Norris
Back To Blog
Saw ____ in ____
Please Enter Your Comments Below
Title :
Comment :
Please enter the text as it is shown in the box below :
Refresh Image
Change For Children
Slacker & Steve
The Latest with Wes, Riggs & Alley
Be More Than a Bystander with Mix 105.1
Be A Mix Freeloader
Text To Win
Mix Internet Radio
My Madison Perks
My Email Offers
Mix 105.1 Mobile Apps
Mix 105.1 on Facebook
Mix 105.1 on Twitter
Mix 105.1 on Pintrest
Last Played List
Recent Blog Posts
OPP Helpful Husband
Slacker And Steve
Dec 12
GMD:Fire the Babysitter
Slacker And Steve
Dec 12
Saw in the Window
Slacker and Steve
Dec 11
Open Line Confessional
Slacker and Steve
Dec 11
OPP: Stay for the Gift
Slacker and Steve
Dec 11
Slacker and Steve
Dec 11
Boomerang Buys
Slacker And Steve
Dec 10
Slacker And Steve
Dec 10
On Today's Show
Dec 2013
Nov 2013
Oct 2013
Sep 2013
Aug 2013
Jul 2013


text:
Popular posts
- Don't waste time to save money
- Why improve nature when destroying it is so much easier?
- About me
- Why present the gender 'pay gap' as a moral issue rather than a profit opportunity?
- Why shouldn't we ration things with queues?
- Steven Pinker on the motivations behind violence
- Why stories celebrate conflict rather than compromise
- NEWS FLASH: multiverse theory proven right
Hi! I am a young Australian man ostensibly interested in the truth and maximising the total number of preferences that are ever satisfied, weighted by their intensity. I also enjoy reading and writing about the topics listed above. If you share my interests, friend me on , , or or subscribe to my RSS feed .
All opinions expressed here are at most mine alone, and have nothing to do with any past, present, future or far future employers.
Some popular posts:
The lives you could have saved
How feasible is a 'charter city of refugees' in Australia?
NEWS FLASH: multiverse theory proven right
Choosing the best status games for society
Eat cows to save mice? Hold your horses!
Beeminding your way to greatness
Should you floss: a cost benefit analysis
What should we do about wilderness?
Twitter Updates
- Your friends have more friends than you on average. Cars in the other lane are going faster on average. #observerselectioneffects 3 hours ago
- RT @sentientist: Solution to gender divide in housework is don't bother-Eventually we'll all be living in perfect egalitarian squalor http… 1 day ago
- RT @WABRENNER: "Okay, people, we've used this equipment to create perfect holes in wood before, so … I think you know the drill." 1 day ago
- RT @ShaunRaviv: Smart @owenbarder thought experiment. Would the price of all aid be worth only the eradication of smallpox? http://t.co/8Lc… 2 days ago
- RT @TimHarford: Why Are Upworthy Headlines Suddenly Everywhere? (Part memetic evolution, part Facebook conspiracy?) dlvr.it/4T8WnP 2 days ago
- RT @tinyrevolution: New logo for US spy satellite from National Reconnaissance Office is weirdly similar to anti-communist propaganda: http… 3 days ago


text:
Dr. Gregory Borg MD
Otolaryngologists — Montgomery, AL
Contact Dr. BorgDr. Gregory Borg specializes in otolaryngology in Montgomery, Alabama. Details of Dr. Borg's 32 years experience as an MD, his 2 hospital affiliations and education at University of Texas Medical School at Houston are available on your free doctor report.
Dr. Borg has been compared
179 times.
Primary Location for Dr. Borg
1722 Pine St
Suite 804
Montgomery, AL 36106
Ratings and Reviews for Dr. Gregory Borg
ExcellentDr. Gregory Borg has an average rating of 5 stars (out of 5) with a rating of Excellent based on 1 reviews.
If you know and have received services from Dr. Gregory Borg, you can rate and review Dr. Gregory Borg by clicking the following button.
Board Certification, Specialty and Expertise
More information available on your free report
Hospital Affiliations
A hospital affiliation covers any doctor who has an affiliation, association or admitting privilege with a hospital. A doctor's particular affiliation to a specific hospital can translate to the overall quality of that provider.
Dr. Borg is affiliated with 2 hospitals:
2105 E S Blvd
Montgomery, AL 36116
See additional Hospital Affiliations
Dr. Borg attended medical school at University of Texas Medical School at Houston and graduated in 1981 having 32 years experience.Get more education details on your free report
While there is no proven correlation between the quality of one's educational institution and the quality of one's professional competence, it is often an indicator. Medical schools have various levels of selectivity (some are harder to get into than others), and both the quality of their clinical training and level of research are measured. Additional medical training in both residency and fellowships and the facilities they are conducted at also provide a history of the doctors' level of training and experience.
Patients may also know Dr. Gregory Borg MD as:
- G Borg
Top searched Otolaryngologists in Montgomery, AL:
We found 5 doctors nearby Dr. Borg who also specialize in Otolaryngology:
We found 5 doctors with names similar to Dr. Gregory Borg:
- Dr. Gregory Borucki
- Dr. Gregory Van den Berghe
- Dr. Gregory Berkey
- Dr. Gregory Borak
- Dr. Gregory Park
Are you Dr. Gregory Borg?
Update your information


text:
PASADENA, Calif. (KABC) -- Fights erupted outside the Pasadena Apple store Friday morning, as the frenzy for the new iPhone 5S and 5C models reached new heights.
Punches were thrown just outside the doors of the Pasadena Apple store. Police stormed in and arrested two men. Later, two women -- one of them pregnant -- got into a brawl on the ground, as their boyfriends faced off in an angry confrontation. One of those men ended up running into the street, throwing himself against a passing truck. He too was detained by police. In total, three people were arrested.
The violence unfolded as hundreds of people stood in line for hours, a handful of them for several days, waiting to buy the new iPhone 5S or the 5C. Another added element this time, aside from the heated brawls, was the fact that 80-100 people in line were shuttled in from Skid Row.
"It ballooned really quickly. In the end, a huge group came in behind us," described one witness.
Get news, traffic and weather on the go with the FREE ABC7 Mobile Apps
Witnesses say the people were driven into Pasadena by a man who promised to pay them $20 in exchange for each iPhone claim ticket they handed him.
"I buy phones and I resell them...for a lot more," said the man, who did not wish to be identified. "It's not illegal. It's not against the law. I'm buying them at full retail price."
Police agree - it's not illegal. But Apple Corporate caught wind of the man's plan and refused to sell him more than about a dozen phones. He, in turn, refused to pay the Skid Row folks who waited in line. That's when the chaos erupted.
"All I know is that we sat here, and now we're not being paid," said one woman.
"He got us down here, we spent the night all night. We got no money, we got nothing," said another person from Skid Row.
The man behind the resell scheme was later confronted by the Skid Row crowd, demanding money and clawing at the phones in his hands. Police had to drive him away for his safety.
Meantime, Apple fans who braved a long night watched in disgust, saying all of it seemed unfair.
"It's annoying to hear all this ruckus all night, yelling, cussing, smoking cigarettes. I feel like it wasn't fair for them to just sit out here, but it happens. It's life," said Dariel Johnson, a Pasadena resident.
Even amid the drama, the die-hard iPhone fans were still excited for the iPhone 5S and 5C.
Francisco Naranjo, who got the first iPhone 5S from the store, came out victorious.
"I think, for me, it's pretty fun," said Naranjo.
The iPhone 5S is available in silver, gold and gray and runs a new chip, the A7, that is up to twice as fast as the A6. It has a fingerprint reader, called Touch ID, so instead of entering a passcode, you can just touch the home button to unlock the phone.
The 5S also has a better camera with larger pixels, called iSight. There's a two-tone flash feature that is designed not to clash with the colors in the room or a person's skin color. The camera also has "auto image stabilization" to avoid blurry pictures and a slow-motion camera for video.
The iPhone 5S starts at $199 with a two-year contract for the 16-gigabyte model.
The iPhone 5C is the less expensive version, available in green, blue, yellow, pink and white. The 5C costs $99 for a 16-gigabyte model and $199 for a 32 gigabyte model with a two-year wireless contract.
Get news, traffic and weather on the go with the FREE ABC7 Mobile Apps
technology, apple, iPhone, pasadena, los angeles news
- NKorea deports captive American war veteran
- Covina home-invasion robbery: girl, dad shot
- Ex-detective arrested for tipping off gang
- Nelson Mandela's legacy honored around world
- 'Valley Bandit' wanted for 8 bank robberies
- Family settles over Chino school gun accident
- Suspected heist reported, 12 tons of pot found
- New Mexico cop fired after minivan shooting
- abcnews: Bill Clinton reveals insights from Mandela
- 6 detained in theft of radioactive material
- Smartphone apps for a better night's sleep
- Long Beach motorcycle show offers demo rides
- 'Out of the Furnace' showcases pacing, acting
- OTRC: Grammys 2014 - list of nominees
Most Popular
Most Viewed StoriesMost Viewed Photos
- abc7.com home
- Site Map
- Advertise with Us
- Contact Us
- Online Public Inspection File
- Technical Help
- ABC.com
- ABCNews.com
- Privacy Policy
- Interest-Based Ads
- Safety Information for this site
- Terms of Use
- Updated Terms of Use
- Copyright ©2013 ABC Inc., KABC-TV/DT Los Angeles, CA. All Rights Reserved.


text:
There are more and more queer characters on television and the silver screen, but the public and critical reaction to Dallas Buyers Club starring Matthew McConaughey illustrates how far LGBT characters and storylines still have to go in Hollywood.
Dallas … Continue reading
John Waters RSS
Fantasia 2012: Excision (2012)
Directed by Richard Bates, Jr
Written by Richard Bates, Jr
Starring AnnaLynne McCord, Traci Lords, Ariel Winter, Roger Bart
82 minutes, English
High school is hell. At least on screen. Everything from Buffy the Vampire Slayer to … Continue reading
John Waters inspires Montreal's new Mascara & Popcorn – but festival not allowed to screen older films by Waters
Montreal's bursting summer festival season gets a new addition beginning today (July 4) with the launch of Mascara & Popcorn, a week-long festival uniting underground and independent filmmakers and artists. Inspired by renegade filmmaker John Waters – a.k.a. The Pope … Continue reading


text:
Blinds 4 U
100% Were impressed
Blinds 4 U offers custom window dressings. Its specialists will come to you by appointment with fabric swatches and samples to match your home decor. Blinds 4 U will also beat any printed installed prices. Installation and measuring is free.
1509 Willowbrook Ln, Villanova, PA 19085
(610) 526-9222
- Accessibility Details: Street level
- Hours: By appointment
- Hours: Mon - Fri, 9:30am - 6pm
- Accessible: yes
Posts awaiting your approval 0
- Bryn Mawr-Gladwyne Patch Editor
- Alison Smith
- firstname.lastname@example.com
Copyright ©2013 Patch. All rights reserved.


text:
Pendragon's Raiment (Any)
Store Price:
Not available in stores
Store Sellback Value: 15,000 gold
Lowest Buy Now Price: 199,999 gold
Average Buy Price: 169,987 gold 0%
I didn't know we had a king...I thought we were a autonomous collective.
You need to be logged in to add this to your wishlist!
0 Listings for Pendragon's Raiment
Item | Buy Now Price | Bid Price | Seller | Time Left | ||
No listings found for this item |


text:
The Graphics Card Waterblock Package
The GPU waterblock spots a very smooth base
The GPU Waterblock is actually full aluminum and fits the old
NVIDIA and ATi graphics cards (such as Geforce Ti4800 and Radeon 9800 Pro)
mounting dimensions, as well as the newer cards (Geforce 6, 7 series and Radeon
X18/19 series). As with the CPU waterblock it took me just a very short while to
read through the manual and undertsand the installation of this waterblock onto
my PowerColor ATi X1900GT. It was a little bit tougher with smaller nuts and
bolts and removing the original cooler on the car took some time too. Time
started 0925hrs. Time finished installation: 0947hrs. Wow! Why did it take so
much longer than the CPU waterblock installation?
All thanks to the transparent small little washers. Or the
lack of one… Well not only were they small, they were transparent as well, and
I dropped one on the floor and had a hell of a time trying to find it. To add to
this, Zalman did not provide any spares, so you kind of get stuck if you lose
just one of them. It would be really helpful if Zalman would just pack in a few
spares, especially with little parts such as these that gets lost easily. Moving
on from here, it was time to install the hardware in my test Case. I used a
Tsunami LEO 46 normal ATX Case:
All in… Time check: 1012hrs. It took 54 minutes
to get to this stage. Here is the full System configuration:
Platform Test Setup | ||
| Zalman Reserator 2 | Default Air-Cooling |
CPU | Intel Core 2 Duo E6700 2.66GHz | |
Motherboard | Gigabyte GA965P DS3 | |
Memory | 2 x 1GB Corsair 8500C5 DDR2 Memory | |
Graphics Card | PowerColor ATi X1900GT 256MB | |
Power Supply | Silverstone Zeus ST75ZF | |
Optical | ASUS 16x DVD Rom | |
Hard Disk | Seagate 80GB Barracuda SATA | |
Chasis | Tsunami LEO 46 ATX Case (Side panel Closed) | |
Operating | Windows XP Professional SP2 |
Latest Headlines
'Entry-level' Galaxy Note 3 Lite begins production, February 2014 launch aimed
Adrian Diaconescu | December 12, 2013It's no secret anymore Samsung is eyeing the further expansion of its Galaxy Note product catalog with a low-cost "Lite" version of the 5.7-inch GNote 3.
- Adrian Diaconescu | December 12, 2013
Curvaceous LG G Flex lands February 2014 in India with $1,000 price tag
- Harish Jonnalagadda | December 12, 2013
Digital Storm's Steam Machine dual-boots SteamOS and Windows, costs $1,469
- Adrian Fonseca | December 12, 2013
Southwest Airlines offers iMessage access in-flight for $2 daily
- Adrian Fonseca | December 12, 2013
Walmart iPhone prices expected to be slashed as part of a holiday sale
- Adrian Diaconescu | December 12, 2013
- CM Storm outs the new Trigger-Z mechanical keyboard
December 12, 2013
The newest addition to its line of gaming keyboards, CM Storm's Trigger-Z offers a number of gamer-oriented features such as programmable macro keys and key profiles, coupled with support for up to 75 macros. CM Storm has unveiled the Trigger-Z mechanical gaming keyboard, the successor to the original Trigger launched last year. Key features include: [...]The post CM Storm outs the new Trigger-Z mechanical keyboard appeared first on VR-Zone Pricewatch.
- [New PVC Figure] Soar high with Alter's Lynette Bishop from Strike Witches
Alter is adding yet another member of the fabled 501st Joint Fighter Wing from Strike Witches into their PVC figure line-up as the lovely British gunner, Lynette Bishop joins in. This 1/8 scale PVC figure measures in at approximately 230mm... Read More »The post [New PVC Figure] Soar high with Alter's Lynette Bishop from Strike Witches appeared first on SGCafe.
- [New PVC Figure] Soar high with Alter's Lynette Bishop from Strike Witches
VR-Zone 中文版
- X99 搭配 DDR4 記憶體,Haswell-E 八核心處理器時脈爲 3.0GHz
Intel 將會在 2014 年第三季發表 Haswell-E 處理器。 將取代現在的 Ivy Bridge-E 以及 Sandy Bridge-E,採用不算新,但無法相容現行 X79 主機板腳位的 LGA 2011-3。 Haswell-E 確定導入八核心處理器,以及 DDR4 記憶體。 與 Ivy Bridge-E 相同,22nm 製程的 Haswell-E 只會擁有「X」與「K」兩個系列,兩款處理器除了可以超時脈外,記憶體部分也被允許超頻。 外觀方面與早前曝光的設計圖相同,在我們收到的 Haswell-E 處理器照片中,可以見到時其時脈爲 3.0GHz,經確認,它是 Haswell-E 處理器系列中最高階的產品,意味著這顆處理器擁有 8 顆核心。 各位也可以注意到,這顆處理器是在美國生產,而非過去常見的馬來西亞或是哥斯達黎加等地,因此我們猜測它應該是相當早期的工程樣品。 X79 與 X99 晶片主要差異應該是後者提供了 10 Ports 的 SATA 6Gb/s,以及不怎麼重要的原生 USB 3.0。 到時候大家比較在乎的部分應該是 DDR4 記憶體的表現,以及 22nm 製程的 Haswell-E 處理器效能,更重要的是 Intel [...]
- X99 搭配 DDR4 記憶體,Haswell-E 八核心處理器時脈爲 3.0GHz
About VR-Zone
VR-Zone is a leading online technology news publication reporting on bleeding edge trends in PC and mobile gadgets, with in-depth reviews and commentaries.
Our inquisitive team of journalists cover key international events like CES, CeBIT, MWC, Computex, E3, and local IT fairs like IT SHOW, PC SHOW, COMEX, CEE and SITEX.
© 1999 - 2013 VR Media Pte. Ltd.


text:
All Pages
Browse Issues
North Shore Times : December 10th 2013
Front Cover
Tuesday, December 10, 2013 Order your copy or go to facebook.com/cloudysunday to order a paperback copy of Cloudy Sunday for $12 plus postage and handling. Classes end Fifty years of community courses have come to an end for one woman as two schools scrap their programmes – P2 Email firstname.lastname@example.com INSIDE Hi-tech moves Albany has been dubbed the country's ''Silicon Valley'' as more high-tech businesses move there – P3 In store Defying odds: BirkdalemanMichael Kyriazopoulos, 38, has written a novel despite losing all mobility from motor neurone disease. Photo:MARYKEPENMAN Novel approach By MARYKE PENMAN NEW author Michael Kyriazopoulos, 38, has written a novel despite losing all mobility from motor neurone disease. The Birkdale man was not even registered with a doctor when he was diagnosed with the disease in January. Doctors warned the keen mountain biker and surfer he had just months to live. Mr Kyriazopoulos set about fulfilling his long-held desire to write a novel soon after processing the gloomy prognosis. Cloudy Sunday was released in paperback and in Kindle version on Amazon. Go to northshoretimes.co.nz and click Latest Edition to see a video on physicist Stephen Hawking who has lived 50 years with the disease. The fictional story follows a 21-year-old member of the Maori Battalion who finds himself separated from his unit in Greece during World War II. Mr Kyriazopoulos has gradually lost the use of his legs, arms and his speech. He is confined to a motorised wheelchair, unable to move and uses A Takapuna Legend Summer has Summer has arrived early atarrived early at........ 5034006AP European Cuisine with a touch of the Mediterranean computer software prompted by his eye movements to communicate. Rapid muscle degenera- tion from the disease forced Mr Kyriazopoulos to be crafty with his writing. ''For as long as I could hold a pen I wrote. ''Then I used a voice recorder on my mobile phone and dictation software on my computer. By the time I got to editing I had to dictate changes to my wife Jo. ''It was incredibly frustrat- ing.'' Fear that he would never get to see his work in print plagued him throughout the entire process, Mr Kyriazopoulos says. 3 COURSE MEAL! Special Dinner Offer $20 20 ''Nobody can give an esti- mate of how long you have, because of the nature of the disease everyone responds differently. ''I was elated to finally see it published.'' Mr Kyriazopoulos who is of Greek heritage studied te reo at Unitec after realising there are many parallels between Maori and his own culture. Constant fatigue, weak- ness in his arms and uncharacteristic clumsiness were the first signs something was wrong, wife Joanne Kyriazopoulos says. A precautionary trip to the doctor turned into an overnight stint in hospital and ENTRÉE Garlic Bread or Soup MAIN Select any from the main menu 2 Course Meal and a Glass of Wine LUNCH Not valid on public holidays. Takapuna only 129 Hurstmere Road, Takapuna | Ph 489 3104 • www.aubergine.co.nz dozens of painful tests, she says. The results of a lumbar puncture, blood test, MRI and nerve exam confirmed the motor neurone disease (MND). ''We didn't even know what it was. I said to Mike, 'is that what Stephen Hawking has?','' she says. There is no known cause or cure for the condition that affects one in 15,000 New Zealanders. Mr Kyriazopoulos says he may be pushing it to pen another novel but he continues to write articles for industry magazines and newsletters, including the monthly MND News. DESSERT Your choice from the dessert menu Not valid on public holidays. Takapuna only Changes in the way people watch movies has led to the closure of a video store – P4 Sugar rush Liz Willis gets a sugar rush when she samples 30 pavlovas in the hunt for the best one in North Shore – P8-9 ONLINE Check out our digital edition for extra, interactive content. Go to northshoretimes. co.nz and click on Latest Edition. $35 Present these coupons to enjoy our Offers. One coupon covers all diners per table Conditions apply. 35 SEVEN DAYS! VALID
December 6th 2013
Next Page


text:
Patent application title: ANTIFOULING METHOD AND COMPOSITION USING CHROMEN-4-ONE DERIVATIVES
Peiyuan Qian (Hong Kong, CN)
Xiaojian Zhou (Hong Kong, CN)
Hongping He (Hong Kong, CN)
Ying Xu (Hong Kong, CN)
IPC8 Class: AB05D500FI
USPC Class:
Class name: Coating processes restoring or repairing
Publication date: 2011-07-21
Patent application number: 20110177237
Sign up to receive free email alerts when patent applications with chosen keywords are published SIGN UP
A method to utilize a chemical genus of chromen-4-one derivatives as
non-toxic, environment friendly antifouling agent, a coating material for
objects submerged under the water and subject to biofouling based on the
chromen-4-one compounds, as well as a method of obtaining one of such
chromen-4-one antifouling compounds, 4',5,7-trihydroxy-isoflavone, from a
natural source. Particularly exemplary of such substituted chromen-4-one
antifouling compounds are provided and they are
4',5,7-trihydroxy-isoflavone, 4',5-dihydroxy-3,6,7,8
tetramethoxy-flavone, 3',5-dihydroxy-3,4',6,8 tetramethoxy-flavone,
4',5-dihydroxy-3,6,7-trimethoxy-flavone, 4',5,7-trihydroxy-flavone,
3',4',6,7-tetrahydroxy-flavone, and 5-monohydroxy-flavone.Claims:
1. A method of preventing or reducing biofouling on a surface of an
object submerged in water, comprising a step of applying a coating
material to said surface of said object, wherein said coating material
comprising at least one substituted chromen-4-one antifouling compound
and a carrier.
2. The method of claim 1, wherein said substituted chromen-4-one antifouling compound is of the following formula: ##STR00012## wherein R5, R6, R7, R8 are independently H, OH, or OCH3; and R2, R3, are independently H, OCH3, Ph, or substituted Ph, provided that when R2 is Ph or substituted Ph, R2 is H or OCH3 and when R3 is Ph or substituted Ph, R2 is H or OCH.sub.3.
3. The method of claim 2, wherein said substituted Ph is: ##STR00013##
4. The method of claim 3, wherein said substituted chromen-4-one antifouling compound is selected from the group consisting of: 4',5,7-trihydroxy-isoflavone, 4',5-dihydroxy-3,6,7,8 tetramethoxy-flavone, 3',5-dihydroxy-3,4',6,8 tetramethoxy-flavone, 4',5,-dihydroxy-3,6,7-trimethoxy-flavone, 4',5,7-trihydroxy-flavone, 3',4',6,7-tetrahydroxy-flavone, and 5-monohydroxy-flavone.
5. The method of claim 4, wherein said substituted chromen-4-one antifouling compound is 4',5,7-trihydroxy-isoflavone.
6. A coating material useful for preventing biofouling according to the method of claim 1, comprising (a) at least one chromen-4-one antifouling agent, (b) a carrier, and (c) a binder for making said chromen-4-one derivative adherent to a surface of an object submerged in water.
7. The coating material of claim 6, wherein said substituted chromen-4-one antifouling compound is of the following formula: ##STR00014## wherein R5, R6, R7, R8 are independently H, OH, or OCH3; and R2, R3, are independently H, OCH3, Ph, or substituted Ph, provided that when R2 is Ph or substituted Ph, R2 is H or OCH3 and when R3 is Ph or substituted Ph, R2 is H or OCH.sub.3.
8. The coating material of claim 7, wherein said substituted Ph is ##STR00015##
9. The coating material of claim 8, wherein said substituted chromen-4-one antifouling compound is selected from the group consisting of 4',5,7-trihydroxy-isoflavone, 4',5-dihydroxy-3,6,7,8 tetramethoxy-flavone, 3',5-dihydroxy-3,4',6,8 tetramethoxy-flavone, 4',5,-dihydroxy-3,6,7-trimethoxy-flavone, 4',5,7-trihydroxy-flavone, 3',4',6,7-tetrahydroxy-flavone, and 5-monohydroxy-flavone.
10. The coating material of claim 9, wherein said substituted chromen-4-one antifouling compound is 4',5,7-trihydroxy-isoflavone.
11. A method of manufacturing a coating material useful for preventing biofouling according to the method of claim 1, comprising a step of mixing at least one substituted chromen-4-one antifouling compound with a carrier and a binder.
12. The method of claim 11, wherein said substituted chromen-4-one antifouling compound is of the following formula: ##STR00016## wherein R6, R7, R8 are independently H, OH, or OCH3; and R2, R3, are independently H, OCH3, Ph, or substituted Ph, provided that when R2 is Ph or substituted Ph, R2 is H or OCH3 and when R3 is Ph or substituted Ph, R2 is H or OCH.sub.3.
13. The method of claim 12, wherein said substituted Ph is: ##STR00017##
14. The method of claim 13, wherein said substituted chromen-4-one antifouling compound is selected from the group consisting of: 4',5,7-trihydroxy-isoflavone, 4',5-dihydroxy-3,6,7,8 tetramethoxy-flavone, 3',5-dihydroxy-3,4',6,8 tetramethoxy-flavone, 4',5,-dihydroxy-3,6,7-trimethoxy-flavone, trihydroxy-flavone, 3',4',6,7-tetrahydroxy-flavone, and 5-monohydroxy-flavone.
15. The method of claim 14, wherein said substituted chromen-4-one antifouling compound is 4',5,7-trihydroxy-isoflavone.
16. A method of producing a substituted chromen-4-one antifouling compound from a natural source, comprising steps of (a) obtaining a bacterial strain; (b) growing said bacterial strain in a fermentation process to yield an amount of cells in a fermentation broth; (c) extracting said cells and fermentation broth with ethyl acetate to afford an extract; (d) concentrating said extract to afford an amount of black oil; and (e) following a bioassay guided isolation process to obtain a substituted chromen-4-one antifouling compound; wherein said bacterial strain is Streptomyces fungicidicus.
17. The method of claim 16, wherein said substituted chromen-4-one compound is 4',5,7-trihydroxy-isoflavone.
[0001] This application claims benefit from Chinese patent application number 200810087953.0, filed Mar. 20, 2008, the content of which is hereby incorporated in its entirety by reference.
[0002] The present invention relates to antifouling methods and materials based on nontoxic bioactive agents. Particularly, it relates to an antifouling method and formulation based on one or more bioactive derivatives of chromen-4-one.
[0003] Biofouling is the undesirable accumulation of micro-organisms, plants, and animals on artificial surfaces immersed in water such as ship hulls, docks, buoys, etc. More than 4000 organisms having been reported as being species causing biofouling, including bacteria, micro-algae, macro-algae, sea-grass, molluscs, crustacean, etc. Most fouling-causing organisms have a swimming larval stage followed by a sedentary adult stage that remains attached to its substratum throughout the remainder of its life. The attached adult organisms can increase frictional resistance on the hulls of ships, increase the weight of buoys, increase oil flat loading weight, block seawater pipes, decrease water exchange through aquaculture net boxes, and compete for space and food with cultured shellfish, among other negative effects.
[0004] The global economic costs due to biofouling are extremely large. For example, biofouling of ships' hulls leads to the high frictional resistance, increase of weight, and subsequent potential speed reduction and loss of maneuverability. As a result, higher fuel consumption is needed, causing increased emissions of harmful compounds. It may also entail a need for heavier and energetically efficient machinery. The estimated economic loss caused by biofouling is around US$ 6 billion annually. The use of a typical antifouling agent (tributyltin) on ship hulls, has saved the US Navy an estimated US$150 million each year. Because of these large-scale economic consequences, development of efficient antifouling methodologies is crucial.
[0005] Traditionally, antifouling materials are metal based. Prior to the 17th century, plumbum was the most common antifouling agent. From 1960's until recently, organotins, represented by tributyltin (TBT), were common anti-fouling agents. However, environmental concerns over the effect of organotins first arose in France, where severe problems were encountered in commercial oyster fisheries in areas where there was intense boating activity and poor tidal exchange. Since then, the distribution, fate and effects of organotins and other antifoulants on the marine and freshwater environment have been under intense scrutiny. Research suggests damaging effects of organotins on reproduction and growth of various marine life. In addition, TBT is known to threaten non-target organisms in the marine ecosystem, causing dramatic effects on shell fishes by inducing imposex in large populations. At concentrations as low as 1 ng/L, TBT can induce imposex. Now, TBT is regarded as one of the most toxic and hazardous compounds introduced into marine environments. In response to these concerns, the Marine Environment Protection Committee (MEPC) of the International Maritime Organization (IMO) has implemented a ban on the application of TBT paints from 1 Jan. 2003, with the intent that no TBT paints will remain on vessels after 1 Jan. 2008.
[0006] As an alternative, vessels are increasingly painted with copper-based paints. However, copper-based paints also have negative effects on the marine environment. For example, oysters accumulate considerable amounts of copper and copper is toxic to marine algae.
[0007] The toxicity concern is not only on TBT, but on all antifouling biocides, and has thus stimulated research and development of non-toxic antifouling coatings. The development of a marine paint or paint ingredient that is non-toxic, non-heavy-metal-based, and benign to the marine environment is urgently sought. The present invention provides a number of environmentally-friendly, natural anti-fouling agents, compositions, and applications.
[0008] One object of the present invention is to provide a chemical genus from which the compound is likely nontoxic but has potent antifouling activities. Another object of the present invention is a method of obtaining such chemical compound from marine micro-organisms.
[0009] This and other objects of the present invention are realized by providing a group of substituted chromen-4-one derivatives, which show a potent antifouling activity but are non-toxic and environmental friendly. As used in this invention, the term "substituted chromen-4-one antifouling compound" means a species of a substituted chromen-4-one genus, which has at least one substituent on each of the two rings (i.e., the benzene ring and pyran ring) of chromen-4-one, of which the backbone is represented by formula I. The substituent on the pyran ring comprises an aromatic ring, preferably a benzene ring, and the substituent on the benzene ring is preferably a hydroxy (OH) or methoxy (CH3O) group.
[0010] Preferable antifouling chromen-4-one derivatives according to the present invention have: (a) an aromatic ring, preferably a benzene or substituted benzene group, attached at position 2 or position 3, preferably at position 2, (b) has a hydroxyl at position 5, and (c) has no bulky substituent group (such as monosaccharide or amylose) attached at position 3 (preferably absence of any substituent) when there is a substituent at position 2. For example, following this guideline, the following substituted chromen-4-one compounds of formula II are particular embodiments of the present invention:
wherein R5, R16, R7, R8 are independently H, OH, or OCH3; and R2, R3, are independently H, OCH3, Ph, or substituted Ph, provided that when R2 is Ph or substituted Ph, R2 is H or OCH3 and when R3 is Ph or substituted Ph, R2 is H or OCH3. Examples of substituted Ph are:
[0011] The chemical genus of chromen-4-one derivatives of the present invention is mostly known but it is unknown that any species in this genus has any antifouling effect. In addition, the present invention also provides a previously unknown source or method of obtaining this compound. It is from a natural source, i.e., a marine species Streptomyces fungicidicus. The compound genus of the prevent invention is advantageous for two reasons. First, marine micro-organisms represent a sustainable source to tackle the current biofouling problems and fermentation requires less manpower and is more environmentally and economically friendly. Second, there are many commercially available derivative compounds in this genus, permitting effective structure-activity relationship analyses. Such analyses can lead to more effective derivative compounds which are more suitable for semi or total chemical synthesis and thus can be produced more economically on an industrial scale. Moreover, being derived from naturally occurring compounds, all the antifouling compounds of the present invention, are heavy-metal free, and very likely nontoxic and decomposable in marine environments.
[0012] As particular non-limiting embodiments of the present invention, the following seven (7) compounds are representatives of the chromen-4-one genus of the present invention as a guideline for the scope that the present invention pertains:
[0013] Compound 1: 4',5,7-trihydroxy-isoflavone (genistein)
[0014] Compound 2: 4',5-dihydroxy-3,6,7,8 tetramethoxy-flavone (calycopterin)
[0015] Compound 3: 3',5-dihydroxy-3,4',6,8 tetramethoxy-flavone (casticin)
[0016] Compound 4: 4',5,-dihydroxy-3,6,7-trimethoxy-flavone (penduletin)
[0017] Compound 5: 4',5,7-trihydroxy-flavone (apigenin)
[0018] Compound 6: 3',4',6,7-tetrahydroxy-flavone (luteolin)
[0019] Compound 7: 5-monohydroxy-flavone (primuletin)
[0020] The seven (7) antifouling compounds as particular embodiments of the present invention, are all within the above defined chemical genus and obtained by the bioassay guided isolation from metabolites of marine micro-organism, or from terrestrial plants by the bioassay confirmed structure-activity relationship analysis. The results of experiments have proved that all the natural compounds in the present invention are nontoxic and promising antifouling compounds. The antifouling paints based thereon are also environmentally safe.
[0021] A further object of the present invention is to provide a cultivation method for producing manufacture chromen-4-one derivatives. As a particular embodiment, a cultivation method is described herewith in detail, which is used to produce 4',5,7-trihydroxy-isoflavone from the marine fungus Streptomyces fungicidicus. Without undue experimentation, people having ordinary skill in the art may apply or modify the method described herein for 4',5,7-trihydroxy-isoflavone in practicing other antifouling chromen-4-one derivatives of the present invention, which may be isolated from natural sources or chemically synthesized and commercially available.
[0022] The foregoing objects of the present invention are in one inventive unit, united by a novel technical feature, that is, the antifouling effect of the substituted chromen-4-one compounds.
[0023] The various features of novelty which characterize the invention are pointed out with particularity in the claims annexed to and forming a part of this disclosure. For a better understanding of the invention, its operating advantages, and specific objects attained by its use, reference should be made to the drawings and the following description in which there are illustrated and described preferred embodiments of the invention.
[0024] FIG. 1 is a flowchart of the bioassay-guided isolation of 4',5,7-tryhydroxy-isoflavone (genistein) from the culture of Streptomyces fungicidicus.
[0025] FIG. 2 shows the inhibitory effect of the compound 4',5,7-tryhydroxy-isoflavone (genistein) on larval settlement of the barnacle Balanus amphitrite. The results are presented as the mean±standard deviation of at least three replicates (n=3).
[0026] FIG. 3 shows hydrolysis of genistein in buffer solutions, artificial seawater and natural seawater. The concentrations were determined by HPLC before hydrolysis (open bar), after 4 days (gray bar), and 7 days (black bar) of incubation at 50° C. in dark for hydrolysis. Tested solutions included artificial seawater (ASW), filtered natural seawater (FSW), and 20 mM acetic buffers of pH 5, 7, and 9. The concentration of genistein was presented as the mean±standard deviation of three replicates (n=3).
[0027] FIG. 4 shows molecular structures and source plants of chromen-4-one derivatives tested. The text above each structure of the compound indicates (a) the sample number, (b) the source plant, and (c) the popular name for the compound, if available.
[0028] FIG. 5 shows the inhibitory effect of the tested samples on larval settlement (%) of the barnacle Balanus amphitrite. The concentration of each sample was at 50 μg ml-1. The results are presented as the mean±standard deviation of at least four replicates (n=4). The statistical differences between the controls and the samples were calculated using Student's paired t-test and indicated at the significance level of: *=p<0.05 and **=p<0.01 on the top of each bar.
A. The Strains Producing Antifouling Compounds
[0029] The strain of Streptomyces fungicidicus was isolated from sediment samples from 5000 m depth in west Pacific Ocean (N10.sup.o50'35''; W154.sup.o05'28''). The samples were kept at 0° C. and 44° C. before and after arriving laboratory, respectively.
[0030] The strain of S. fungicidicus was isolated by the following enrichment techniques. Sediment samples (about 1 g) were suspended in 10 ml artificial seawater. After mixing, 200 μl of the suspension was spread on 2216E agar plate and incubated at 10° C. Colonies with different shape or color were transferred to a new agar plate for new incubation and further isolation. The final identification was conducted by detection of 16S rRNA sequence (SEQ ID No: 1). 16S rRNA sequence was amplified by the primers 8F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1525R (5'-AGAGTTTGATC CTGGCTCA-3'). Sequencing reactions were performed by using forward primers 26F (5'-AGAGTTTGATC CTGGCTCA-3') and 355F (5'-A CTCCTACGGGAGGCAGC-3') and reverse primers 1492R (5'-GGYTACCTTGTTACGAC TT-3') and 1525R (5'-AAGGAG GTGWTCCARCC-3'), SEQ ID Nos: 2-7, respectively. The sequence was deposited in Genbank under accession NO. AY636155.
[0031] The sequence was analyzed for similarity with other known sequences with BLAST program (National Center for Biotechnology Information (NCBI), www.ncbi.nlm.nih.gov/BLAST/). BLAST results showed that the strain is Streptomyces fungicidicus strain YHO4 (Accession no. AY636155, identity=99%).
B. Isolation and Identification of Antifouling Compound from Marine Micro-Organism
[0032] A Streptomyces fungicidicus strain was inoculated into culture media of pH 7.8 containing 20 g malt extract, 8 g Yeast extract, 8 g glucose, and 1.5 L filtered seawater. About 60 L cell suspensions were collected after the strain was incubated for 10 days by 110 rpm shaking. Cells were separated from fermentation broth by centrifugation and both subjected to triplicate ethyl acetate extractions separately. About 8 g black oil was obtained after collecting all extraction and volatilization of solvent, and was subjected to the isolation and identification as described in FIG. 1.
[0033] The structure of this compound was identified by NMR (1HNMR, 13CNMR, DEPT and COSY). The molecular weight of the compound was determined by negative ESI-MS (Waters Micromass ZQ ESI-MS). ESI-MS: [M-H]-m/z 269. The detailed data for 1H NMR and 13C NMR spectra are listed as following. 1H NMR (400 MHz, DMSO-d6) δ: 12.97 (C5-OH), 10.91 (C4'-OH), 9.61 (C7-OH), 8.33 (s, 1H, H-2), 7.38 (2H, d, J=8.5 Hz, H-2',6'), 6.82 (2H, d, J=8.5 Hz, H-3',5'), 6.39 (s, 1H, H-8), 6.23 (s, 1H, H-6). 13C NMR (100 MHz, DMSO-d6) δ: 93.5 (C-8), 98.8 (C-6), 104.3 (C-10), 114.9 (C-3', 5'), 121.0 (C-3), 122.1 (C-1'), 130.0 (C-2', 6'), 153.9 (C-2), 157.3 (C-9), 157.4 (C-5), 161.8 (C-4'), 164.1 (C-7), 180.1 (C-4).
[0034] Based on above data, the compound was identified as: 4',5,7-tryhydroxy-isoflavone (genistein):
Other compounds can be made according to publicly known methods and/or from commercial sources. For example, compound 2 can be prepared according to T. R. Seshadri and V. Venkateswarlu. A synthesis of calycopterin. Proceedings of the Indian Academy of Sciences, Section A. Volume 24, 1946, Pages 349-351, and compound 4 can be synthesized according to S. E. Flores, J. Herrin and H. Menchaca. The synthesis of penduletin. Tetrahedron. Volume 4, Issues 1-2, 1958, Pages 132-134. Compounds 1, 5, 6, and 7 can be bought from Sigma-Aldrich (6000 North Teutonia Avenue, Milwaukee, Wis. 53209, Phone: 1-800-231-8327). The commercial products are detailed as follows:
[0035] Compound 1
Name: Genistein; IUPAC: 5,7-dihydroxy-3-(4-hydroxyphenyl)-4H-chromen-4-one; MF: C15H10O5; CAS #: 446-72-0; and Prod. # G6649, G6776.
[0036] Compound 5
Name: Apigenin; IUPAC: 5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-chromen-4-one; MF: C15H10O5; CAS #: 520-36-5; and Prod. # A3145, 10798.
[0037] Compound 6
Name: Luteolin; IUPAC: 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-chromen-4-one; MF: C15H10O6; CAS #: 491-70-3; and Prod. # L9283.
[0038] Compound 7
Name: 5-Hydroxyflavone; IUPAC: 5-hydroxy-2-phenyl-4H-chromen-4-one; MF: C15H10O3; CAS #: 491-78-1; and Prod. # H4405.
[0039] Compounds 3 can be obtained from Chengdu Biopurify Phytochemicals Ltd. (Address: 2F, No. 11 Building, No. 388 Rongtaidadao CNSTP, Wenjiang Zone, Chengdu, Sichuan, China, 611130, Tel: +86-28-66780410, Fax: +86-28-82633165). The commercial product is: Name: Casticin (Vitexicarpin); IUPAC: 3',5-Dihydroxy-3,4',6,7-tetramethoxyflavone; MF: C19H18O8; CAS #: 479-91-4; and Prod. # C07067.
C. Bioassay for Antifouling Activity of Genistein Against Larval Settlement
[0040] Larvae were obtained from the adult barnacles B. amphitrite collected from the intertidal zone in Hong Kong (22.sup.o19'N, 114.sup.o16'E). The larvae were reared in freshly filtered natural seawater (FSW, 0.45 μm, 32S salinity) and fed with Chaetoceros gracilis Schutt at 24° C. in the Coastal Marine Laboratory, the Hong Kong University of Science and Technology (HKUST). Larvae that were competent to settle (called cyprids) were stored for 2 days at 8° C. prior to being used in the bioassays. Ten μl of each test sample dissolved in dimethyl sulfoxide (DMSO) were transferred into a well in 24-well polystyrene plate after the addition of 1 ml of FSW. The control wells contained 1 ml of FSW and 10 μl of DMSO. About 15 cyprids were added in each well with triplicate samples being tested. The numbers of settled juveniles, dead larvae, or moving larvae in each well were counted after 24 h of incubations at 24° C. in the dark to calculate EC50 or LC50.
[0041] As shown in FIG. 2, the inhibitory activity of genistein against larvae settlement was observed at a concentration as low as 0.5 μg/ml. In the concentration of 8 μg/ml, 10% of the larvae settled, which is significantly less than in the control (90% settlement). According to the method of Rittschof et al. (1994), the EC50, LC50 and LC50/EC50 values of genistein were measured as 3.0 μg/ml, >50 μg/ml, and >16.7, respectively. The results indicate the present compound are nontoxic since its LC50/EC50 value is higher than 10 (Avelin et al. 1993), while LC50/EC50 values for toxic heavy metal is around 1.
D. Hydrolysis Experiment of Genistein
[0042] The experiments were carried out according to the method described in ASTM (1993). Buffer solutions consisting of 20 mM of sodium acetate were prepared with adjusted pH level at 5, 7, and 9. FSW was the same as those used in bioassays. Artificial seawater (ASW) was prepared as van't Hoff formula (460 mM NaCl, 10.1 mM KCl, 9.2 mM CaCl2, 35.9 mM MgCl2, 17.5 mM MgSO4, 10 mM Tris-HCl, and pH 8.2). Test solution (900 ml) containing 4 μg genistein was divided into nine test glass tubes (100 ml each), and then the tubes were tightly capped. Immediately after preparation of the test solutions, three tubes were loaded onto a Sep-Pak tC18 ENV column (Waters, USA), preconditioned for use by eluting it with methanol and water under reduced pressure. The column after loading the test sample was eluted with water to remove any salts in the test samples, and was then eluted with 2.5 ml of DMSO. Thus, the eluate was 40 times more concentrated than the sample in the original test solution; this concentrated solution was subjected to HPLC analysis. The HPLC system (600, Waters, USA) was equipped with a photodiode array detector (996, Waters, USA) installed in tandem after outlet of the column (Phenomenex Luna 5λ C18 100A, 250×4.60 mm, 5 micron). The solvents used were (A) 5% acetonitrile and (B) 95% acetonitrile at the isocratic flow rates of (A) 0.53 ml min-1 and (B) 0.47 ml min-1 at 40° C., respectively. A volume of ten μl of elute was injected into the column using an autosampler (717plus, Waters, USA). The UV absorption at 1=260 nm was used for quantification. The analytical data were shown as the average value of triplicate analyses. The remaining six tubes were kept at 50° C. in the dark in an incubator for a period of four or seven days according to ASTM Procedure E895-89 (ASTM, 1993). After the incubations, each of three test samples was subjected to HPLC analysis, following the same concentration process as described above to determine the remaining concentration of genistein.
[0043] As shown in FIG. 3, the concentration of genistein significantly reduced after being stored in 50° C. for a week. About 27, 31, 37, 35, and 26% of genistein were decomposed in pH 5.0, 7.0, and 9.0 buffer solutions, ASW, and FSW, respectively. These results indicate that genistein can be decomposed in marine environments and the accumulation of genistein would not seriously occur in marine environments.
E. Field Experiment of Genistein
[0044] Test compounds (genistein, Cu2O, or their mixture) were mixed with resin-based paint (Permoglaze, Akzo Nobel Decorative Coatings, Lancashire, UK) with addition of 15% of (w/w) xylene, and then sprayed on the surface of PVC panels (size: 10 cm×10 cm×0.5 cm). Genistein was incorporated into paint by 10 and 20% of the dry weight as the low and high concentrations, respectively. In the experiments of combination effects, both Cu2O and genistein at 10% of dry weight were added. The controls used was a resin based paint with addition of 15% (w/w) of xylene only. Each of the tested paints was mixed for more than 8 h before being spread onto panels. The thickness of each paint film was between 100 and 150 μm. The painted panels were air-dried completely prior to being tied to PVC frames and deployed vertically at a depth of 1-1.5 m at the pier of HKUST from March to June in 2007. The numbers of adult barnacle B. amphitrite on the surface were counted and the density on each panel was calculated after their submergences. Four replicates were tested for each sample. As shown in Table 1, the statistical analysis clearly showed that the presence of genistein on the painted panel surfaces significantly reduced barnacle fouling (Student's T-test: p<0.05). The statistical analysis demonstrated the clear difference between the single and mixed compounds even after three months of submergence (Student's T-test: p<0.01). The mixture achieved much better antifouling performance than single compound, indicating that genistein has the potential to be used as mixture with other antifoulants.
TABLE-US-00001 TABLE 1 Submergence Control 10% genistein 20% genistein One month 0.32 ± 0.039 0.12 ± 0.031 ** 0.08 ± 0.027 ** Two months 0.71 ± 0.053 0.57 ± 0.055 * 0.13 ± 0.029 ** 10% Cu2O + 10% Cu2O 10% genistein 10% genistein Three months 0.81 ± 0.06 ** 0.77 ± 0.042 ** 0.39 ± 0.053
Table 1: The density of barnacles fouled on the test panels. The content of compounds was the percentage of the compound weight per dried weight of paint. Control paint did not contain Cu2O or genistein. The densities (adults cm-2) of the barnacle Balanus amphitrite on the surfaces of panels were denoted as the mean±standard deviation of four replicate panels. The statistical differences (n=4) between the controls and the treatments, and between single Cu2O, genistein, and their mixture were calculated using Student's paired t-test and indicated at the significance level of: *=p<0.05 and **=p<0.01.
F. Screening for Bioactive Chromen-4-One Derivatives
[0045] Larvae were obtained from the adult barnacles B. amphitrite collected from the intertidal zone in Hong Kong (22.sup.o19'N, 114.sup.o16'E). The larvae were reared in freshly filtered nature seawater (FSW, 0.45 μm, 32S salinity) and fed with Chaetoceros gracilis Schutt at 24° C. in the Coastal Marine Laboratory, the Hong Kong University of Science and Technology (HKUST). Cyprids (competent larvae) were stored for 2 days at 8° C. (aged cyprids) prior to being used in the bioassays. Ten μl of each test sample dissolved in dimethyl sulfoxide (DMSO) were transferred into a well in 24-well polystyrene plate after the addition of 1 ml of FSW. The control wells contained 1 ml of FSW and 10 μl of DMSO. About 15 cyprids were added in each well with triplicate samples being tested. The numbers of settled juveniles, dead or the moving larvae in each well were counted after 24 h of incubations at 24° C. in the dark to calculate EC50 or LC50.
[0046] As shown in FIG. 5, 12 among 17 kinds of chromen-4-one derivatives had a significant antifouling activity against larval settlement (**: Student's paired t-test: p<0.01). The antifouling activity was determined according to the method of Rittschof et al. (1994).
[0047] In Table 2, genistein was purified from extract of Genista tinctoria, a plant species. It was also shown to have the antifouling activity with EC50=3.0 ug/ml and LC50/EC50>16.7, which is exactly the same as that from marine bacteria Streptomyces fungicidicus. The above data also demonstrate that genistein is a nontoxic antifouling compound according to the standard of Avelin et al. (1993) since its LC50/EC50 was detected as >16.7.
TABLE-US-00002 TABLE 2 The EC50 and LC50/EC50 ratio of potential antifoulants against larval settlement of the barnacle Balanus amphitrite. Samples Chemical name EC50 LC50/EC50 F-1 calycopterin. 3.4 ± 0.5 >14.7 F-2 7-hydroxy-8,4'- 13.3 ± 2.5 >7.50 dimethoxyisoflavone F-3 casticin 3.0 ± 0.4 >16.7 F-4 5,4'-dihydroxy-3,6,7- 2.5 ± 0.3 >20.0 trimethoxyflavone F-5 neoisorutin 40.1 ± 3.4 >5.0 F-6 kaempferol 9.6 ± 1.4 >5.2 F-8 3,5-dihydroxy-7,4'- 11.8 ± 2.1 >4.2 dimethoxyflavone F-9 apigenin 3.1 ± 0.3 >16.1 F-10 luteolin 3.8 ± 0.4 >13.2 F-12 genistein 3.0 ± 0.3 >16.7 F-16 icariin 35.3 ± 4.2 >2.8 F-17 primuletin 2.8 ± 0.3 >17.9 For each compound, the EC50 value presented here is mean ± standard deviation of at least three replicates; the LC50 value used for calculation of LC50/EC50 is the highest concentrations causing <50% death of larvae. The other 5 flavone and isoflavone derivatives EC50 with larger than 50 μg ml-1 were not included.
[0048] As shown in Table 2 and FIG. 5, six out of the 12 chromen-4-one derivatives showed almost no toxicity but have the similar antifouling activity as genistein with EC50 around 3.0 ug/ml. These 6 compounds are 3',5-dihydroxyl-3,6,7,8-tetramethoxy-flavone (F-1, calycopterin), 3',5-dihydroxyl-3,4',6,7-tetramethoxy-flavone (F-3, casticin), 4',5-dihydroxy-3,6,7-trimethoxy-flavone (F-4), 4',5,7-trihydroxy-flavone (F-9, apigenin), 3',4',5,7-tetramethoxy-flavone (F-10, luteolin), and 5-monohydroxyl-flavone (F-17, primuletin). The structure and source of the compounds are shown in FIG. 4.
[0049] The above 7 compounds can be utilized as examples of environmentally-friendly antifoulants, which also can be chemically modified to achieve high activities or to reduce cost. Using only ordinary skill in the art, other substituted chromen-4-one antifouling compounds can be made by adding or removing one or more substituent groups, according to the following guideline:
[0050] There should be at least one substituent on each of the two rings (i.e., the benzene ring and pyran ring) of chromen-4-one. The substituent on the pyran ring should be a substituted or unsubstituted aromatic ring group. Up to 4 substituents can be attached to the benzene ring of chromen-4-one and should first consider a hydroxy (OH) or methoxy (CH3O) group as candidates for substituents at the benzene ring before consider other type of suitable substituent groups. If there is substituent attached at position 2 of the pyran ring, then it should be considered to leave position 2 of the pyran ring unsubstituted or substituted with a less bulky group.
[0051] While there have been described and pointed out fundamental novel features of the invention as applied to a preferred embodiment thereof, it will be understood that various omissions and substitutions and changes, in the form and details of the embodiments illustrated, may be made by those skilled in the art without departing from the spirit of the invention. The invention is not limited by the embodiments described above which are presented as examples only but can be modified in various ways within the scope of protection defined by the appended patent claims.
Sequence CWU
711425DNAStreptomyces fungicidicus 1gtcgaacgat gaaccgcttt cgggcgggga
ttagtggcga acgggtgagt aacacgtggg 60caatctgccc tgcactctgg gacaagccct
ggaaacgggg tctaataccg gatatgaccg 120tctgccgcat ggtggatggt gtaaagctcc
ggcggtgcag gatgagcccg cggcctatca 180gcttgttggt gaggtagtgg ctcaccaagg
cgacgacggg tagccggcct gagagggcga 240ccggccacac tgggactgag acacggccca
gactcctacg ggaggcagca gtggggaata 300ttgcacaatg ggcgaaagcc tgatgcagcg
acgccgcgtg agggatgacg gccttcgggt 360tgtaaacctc tttcagcagg gaagaagcga
aagtgacggt acctgcagaa gaagcgccgg 420ctaactacgt gccagcagcc gcggtaatac
gtagggcgca agcgttgtcc ggaattattg 480ggcgtaaaga gctcgtaggc ggcttgtcac
gtcggttgtg aaagcccggg gcttaacccc 540gggtctgcag tcgatacggg caggctagag
ttcggtaggg gagatcggaa ttcctggtgt 600agcggtgaaa tgcgcagata tcaggaggaa
caccggtggc gaaggcggat ctctgggccg 660atactgacgc tgaggagcga aagcgtgggg
agcgaacagg attagatacc ctggtagtcc 720acgccgtaaa cggtgggcac taggtgtggg
caacattcca cgttgtccgt gccgcagcta 780acgcattaag tgccccgcct ggggagtacg
gccgcaaggc taaaactcaa aggaattgac 840gggggcccgc acaagcggcg gagcatgtgg
cttaattcga cgcaacgcga agaaccttac 900caaggcttga catacaccgg aaacgtctgg
agacaggcgc ccccttgtgg tcggtgtaca 960ggtggtgcat ggctgtcgtc agctcgtgtc
gtgagatggt tgggttaagt cccgcaacga 1020gcgcaaccct tgtcccgtgt tgccagcagg
cccttgtggt gctggggact cacgggagac 1080cgccggggtc aactcggagg aaggtgggga
cgacgtcaag tcatcatgcc ccttatgtct 1140tgggctgcac acgtgctaca atggccggta
caatgagctg cgataccgtg aggtggagcg 1200aatctcaaaa agccggtctc agttcggatt
ggggtctgca actcgacccc atgaagtcgg 1260agtcgctagt aatcgcagat cagcattgct
gcggtgaata cgttcccggg ccttgtacac 1320accgcccgtc acgtcacgaa agtcggtaac
acccgaagcc ggtggcccaa ccccttgtgg 1380gagggagctt gtcgaaggtg ggactggcga
ttgggacgaa gtcgt 1425220DNAArtificial Sequenceprimer
2agagtttgat cctggctcag
20319DNAArtificial Sequenceprimer 3agagtttgat cctggctca
19418DNAArtificial Sequenceprimer
4actcctacgg gaggcagc
18520DNAArtificial Sequenceprimer 5cacgagctga cgacagccat
20619DNAArtificial Sequenceprimer
6ggytaccttg ttacgactt
19717DNAArtificial Sequenceuprimer 7aaggaggtgw tccarcc
User Contributions:
Comment about this patent or add new information about this topic:


text:
We'd love to share your voice here at the blog. Especially if […]
by Ken Blanchard , Spencer Johnson , Eric Conger
Other Sellers available from ~ $10.16
by Napoleon Hill , Joe Slattery
Other Sellers available from ~ $10.65
by Jane O'Connor , Isabel Keating
by L. Frank Baum , Robin Field
Other Sellers available from ~ $6.79
by Cedarmont Kids , Sue Martin , Mike Gay
Other Sellers available from ~ $7.82
by James Allen , Ray Porter
Other Sellers available from ~ $10.71
by Lisa McCourt , Cyd Moore , Kirsten Krohn
by Napoleon Hill Foundation , Napoleon Hill Foundation , Credit No
Other Sellers available from ~ $9.84
by Karen Schmidt , Bruce B. Johnson , Doug Califano
by Michael Bond , Stephen Fry
Note:You Buy We Scan
You have chosen eDelivery which means that you are requesting a digital conversion of your physical book order, not the physical book. By clicking Yes below and completing the transaction you are taking ownership of the book after which Better World Books will begin the process of scanning on your behalf. The physical pages are recycled immediately after scanning so no refunds or cancellations are possible when you choose the eDelivery option.
Do you want to proceed?
Close Window
eDelivery Title
Note: You have selected the eDelivery Option
You have selected the eDelivery option for your book, which means that you are requesting the scan of your physical book order. Converting this book to a digital version uses up one physical copy of the book from our inventory and can not be undone. This process will be queued up immediately after you submit your order to ensure prompt delivery. Refunds and cancellations are not possible when ordering eDelivery of your book.


text:
Trainers Near You Ashburn VA change location
Laura Bentley Springfield, VA
Luke Davis Alexandria, VA
Kathryn Augustine Fairfax, VA
ACE Fit Share
Fit Life Articles
There is no question that deadlifts are awesome for building strength and power in the legs, glutes and even the core. Unfortunately, if done incorrectly, they can increase...
Do you look forward to the holidays with anticipation and joy? Or are you more likely to view this season with dread and anxiety as you struggle to stay on track with your...
"Tone up those abs! Melt that fat! Feel the burn!" Sound familiar? Phrases like these are common in fitness settings, but do little to help people reach their health and...


text:
| ||
Item : HM-4218 | |
He's handsome, talented, and oh so smart. Help your little boy look the part in this dapper long sleeved white tee shirt wrought from premium cotton with long sleeves made for comfort and warmth. A classic high rounded neckline and shallow v-cut polo sides add some subtle character, too, but the focal point of this great shirt are the words, "Lil Rockstar" printed in a vintage black font front and center to let the world know just how cool your little guy really is. Pair this shirt with jeans, khakis, or some great black leggings for a look that's sure to charm everyone he meets. | |
complete the look!


text:
Wednesday, December 11, 2013
Latest local news, weather and high school sports for Northern Michigan
School Closings
2014 Olympics
Your Health Matters
Fact Finder
Education Matters
View Stories by Category
Recent Stories
Northern Michigan
Northern Michigan Videos
Northern Michigan Photos
Evening Show
7&4 News Today
Financial News
Fact Finder
Consumer News
Water Issues
Tourism News
Strange News
Political News
Military News
Your Health
The Environment
Election News
Education News
The Economy
Crime Report
Business News
Automotive News
Recent Stories
Latest news from around Northern Michigan, the State of Michigan and the Nation/World
Coast Guard Cutter Mackinaw completes run from Cheboygan to Chicago
Friday, December 06, 2013
A U.S. Coast Guard ship loaded with more than 1,200 Michigan Christmas trees has arrived in downtown Chicago.
read story
Christmas tree ship set to deliver holiday cheer
Monday, November 25, 2013
Each winter, the crew of the Coast Guard Cutter Mackinaw load the ship full of trees and travel from Cheboygan to Chicago to make their delivery.
read story
watch video
13 Marines get Chicago surprise as they head home
Wednesday, November 06, 2013
First-class passengers gave up their seats so the Marines could sit together in First Class.
read story
2 men charged in mass shooting at Chicago park
Tuesday, September 24, 2013
Police believe as many as three people opened fire on a park basketball court, injuring 13 people, including a 3-year-old boy.
read story
Missing teens charged and put on tethers
Monday, September 09, 2013
The missing 14-year-olds who were found Sunday in Chicago were in Isabella County probate court Monday morning.Jayden Thomas of Clare and Braxton Wood of Mt. Pleasant were charged with being runaways. Both pleaded not guilty to the charges.
read story
view photos
watch video
Teens found safe and sound in Chicago
Sunday, September 08, 2013
Two teenagers that ran away from home last month have been found safe and sound in Chicago.
read story
view photos
Calm weather slows Lake Michigan yacht race
Monday, July 15, 2013
Thousands of sailors on more than three hundred yachts are sailing across the Great Lakes to Mackinac Island.
read story
watch video
Ebert: Not just a critic, but a part of Hollywood
Friday, April 05, 2013
Ebert was not just a critic to filmmakers, but a part of Hollywood itself
read story
view photos
watch video
Winter doldrums will give way to weekend rain
Wednesday, March 06, 2013
The best chance for a little bit of light snow will be along/north of highway M-72 into the Eastern U.P. where 1-2" of snow is possible by Thursday morning, but many spots will see less than an inch total.
read story
Skies cloud over with freezing drizzle in the forecast tonight
Tuesday, March 05, 2013
The main concern locally Tuesday night/Wednesday morning would be for patchy areas of freezing drizzle.
read story
Snow to swipe parts of Michigan tonight
Tuesday, February 26, 2013
Another in a series of winter storms will plow across parts of Michigan Tuesday night/Wednesday, but the heaviest snow will fall across Southern Michigan.
read story
Dennis Rodman worms his way into North Korea
Tuesday, February 26, 2013
Piercings and all, Rodman worms his way into North Korea in flamboyant bid for basketball diplomacy
read story
Storm that slammed the plains arriving in Northern Michigan
Friday, February 22, 2013
After a brief respite in the weather, another round of snow will cross Northern Michigan along the decaying remains of a storm that slammed the Central Plains.
read story
watch video
Significant ice and snow storm pounding the plains
Thursday, February 21, 2013
The good news for those with travels is that while it does cloud over by Friday, only pockets of light snow and snow showers are forecasted with most of the area seeing under two inches of snow Friday into Saturday.
read story
Inauguration honor student killed by Chicago gang members
Tuesday, February 12, 2013
Chicago police say two gang members charged in the death of a 15-year-old honor student mistook her and her friends for members of a rival gang.
read story
view photos
watch video
more stories
Up North Live
Get news and weather notifications on your phone by downloading the iPhone or Android app below
Sign up to get alerts and updates for breaking news, severe weather, and deals:
Special Features
Connecting You To The Experts
Health Connections
Best Bets Recreation/Travel
Connecting You To The Experts
Health Connections
Team Bob's
Heating Tips
Find it, click it, use it... your neighborhood business guide.
Culture & Entertainment
Financial Services
Gifts & Retail
Health & Wellness
Health Connections
Home & Garden
Recreation & Travel
Senior Living
Around the Web
Congress readies for a year-end dash
Wall Street Journal
Scores of world leaders to attend Mandela memorial
New York Times
North Korea confirms purge of Kim Jong Un's uncle, describes 'anti-state' crimes
Washington Post
Singapore rocked by first major riot in 44 years
NBC News
Video Headlines
NASA: Cooling Pump on Space Station Shuts Down
Bipartisan Negotiators Reach Modest Budget Pact
Baruch College freshman dies after fraternity 'ritual' goes wrong
Raw: Top Senate Staffer Part of Child Porn Probe
'12 Years,' 'Breaking Bad' Top SAG Nominees
More videos
Most Popular
Most Read
Most Commented
Traverse City man killed in rollover crash
Bond set at a quarter of a million dollars for Wendy's robbery suspect
Employee fired after helping put out van fire
Crews responding to fire in Copemish
Cheboygan man arrested after making false bomb threat
Popular on Facebook


text:
Browse our mobile site for the best experience on your device.
Mobile's Best Country!
Listen to 95KSJ Live
Follow @95KSJ
Dan & Shelby
Bill Black
Jason Taylor
Tige Rodgers
Blair Garner
Scot Chestnut
Chris Randolph
Sunday Morning Gospel
Bob Kingsley
Angie Ward
Country Top 30 With Bobby Bones
Homegrown Country
Dan's Football Page
Most Recently Played
Most Played This Week
Music Videos
NEW! Discover & Uncover
95KSJ Performance Studio
Current Contests
Create an Account
Edit Your Account
Listen on iHeartRadio
Join us on Facebook
Events Calendar
St. Jude Children's Research Hospital
Community Involvement
Jobs with 95KSJ
Internship Opportunities
1/2 Off Deals
Ask the Expert
Charlie Feathers
Charlie Feathers
Get with It: The Essential Recordings (1954-1969)
Original Release Date: July 21, 1998
Track Listing
1. I've Been Deceived
2. Peepin' Eyes
3. Someday You Will Pay
4. Defrost Your Heart
5. Wedding Gown of White
6. Get With It
7. Tongue-Tied Jill
8. One Hand Loose
9. Can't Hardly Stand It
10. Bottle to the Baby
11. Everybody's Lovin' My Baby
12. Too Much Alike
13. When You Come Around
14. When You Decide
15. Nobody's Woman
16. Jungle Fever
17. Why Don't You
18. Dinky John
19. South of Chicago
20. Nobody's Darlin'
21. Deep Elm Blues
22. Runnin' Around [#]
23. So Ashamed [#]
24. Honky Tonk Kind [#]
25. Frankie and Johnny [#]
26. Corrine, Corrina [#]
27. We're Getting Closer to Being Apart [#]
28. The Man in Love [#]
29. Johnny Come Listen [#]
30. Talkin' 'Bout Lovin' [#]
31. Early in the Morning [#]
32. Don't Let Me Cross Over [#]
33. Where's She at Tonight [#]
34. Don't You Know [#]
35. Wild Side of Life [#]
36. Release Me (And Let Me Love Again) [#]
37. Feel Good Again [#]
38. Defrost Your Heart [Alternate Version][#]
39. I've Been Deceived [Alternate Version][#]
40. Bottle to the Baby [Alternate Version][#]
41. Bottle to the Baby [Alternate Version][#]
42. Bottle to the Baby [Alternate Take][#]
Jimmie Davis: Composer
Charlie Feathers: Guitar, Arranger, Vocals, Spoons, Interviewee
Bill Black: Bass
Junior Kimbrough: Guitar, Vocals
Johnny Bernero: Drums
Johnny Bragg: Vocals (Background)
Bill Cantrell: Fiddle
Quinton Claunch: Guitar
Jim Dickinson: Liner Notes, Research
William Diehl: Bass
Omar Dykes: Composer
Colin Escott: Liner Notes, Research
Bubba Feathers: Guitar
Peter Guralnick: Liner Notes, Research
Buddy Harman: Drums
Roland Janes: Guitar
Stan Kesler: Fiddle, Guitar (Steel)
Ramon Maupin: Drums
Bruce Nemerov: Transfers, Digital Transfers
Marcus Van Story: Bass
Maggie Miller: Vocals
Johnny Black: Bass
Scott Colburn: Mastering, Transfers, Digital Transfers
Nick Tosches: Liner Notes, Research
Jody Chastain: Bass, Guitar (Steel), Photography
Robert Gordon: Interviewer
Jeff Hunt: Art Direction, Design
Public Domain: Composer
Susan Archie: Artwork, Design, Layout Design
Jerry Huffman: Guitar
Jimmy Swords: Drums