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Ferrari 550 Maranello--Video Test Drive with Chris Moran http://www.SupercarNetwork.com A sexy black Ferrari 550 Maranello...complete with Tubi Exhaust! Presented by D&M Motorsports and hosted by Chris Moran Marking a return to a front-engined layout for flagship sporting Ferraris, the 550 Maranello sparked a revival of more usable Ferrari models. With a layout harking back to the Daytona of the Seventies, the 550 backed up its position by carrying a stick the size of a giant redwood. With 485bhp to call upon, it was at least two seconds quicker around the Fiorano test track than the Ferrari F355 itself no slouch. With used models now starting to appear, can a 550 Maranello justify itself on any rational basis? Find out here. Leaving aside the question of depreciation, performance or running costs, the less tangible aspects of Ferrari ownership come to the fore with the 550. Take the interior for starters. Theres a refreshing lack of pretension in its execution, a firm understanding of Ferrari heritage and whats necessary and whats mere frippery. No pompous walnut veneer here, no mock-F1 style carbon fibre. Nor are there modern niceties like steering wheel stereo controls, dimming mirrors or cup holders. Can you imagine the late Enzo Ferrari ever specifying cup holders in one of his cars? The very thought of trying to drink while at the helm of a Ferrari. The great man would turn in his grave. No, a Ferrari cockpit should always be designed for driving - and this one is. That doesn't stop it from being an automotive work of art, however, swathed in leather with careful chrome touches here and there. Take the polished gear-knob - it looks and feels extraordinary. Alone, it must be worth £500; or maybe even £1,000 - who can tell? Though the exterior styling may be more Beckham than Barrichello, this is a car for people who love Ferraris and don't mind others knowing. Perhaps it is this more than anything else that marks the 550 Maranellos greatness. You can't imagine someone who might otherwise consider, say, a Mercedes SL or a Jaguar XK8 buying this car; it's too wild - too extreme. But, unlike the F360 Modena, not so wild and extreme that you couldn't really use it as everyday transport. Which, by almost any justification, is about as good as it gets. That the Ferrari 550 Maranello can now be had for five figures rather than six is probably not going to prompt a buying stampede, but it nonetheless makes the car look conspicuously good value against admittedly newer rivals. In many ways, the beautiful thing about the 550 Maranello is that with a private plate on, only a true Ferrari anorak would ever know which model year it was. When used valuations vary by over £50,000, theres a pragmatic sort of appeal to this course of action. Opening values for a 550 are around £43,000 for a 1997 P-registered car, whilst a more recent 2001 X-plated model will fetch in the region of £62,000. Insurance? Youd be disappointed if it were anything other than Group 20 The 550 Maranello is an astonishingly reliable car. In order to demonstrate its durability, a team of British journalists drove one from Buenos Aires to Tierra del Fuego, a trip of over 3000 miles on a mixture of tarmac, snow and dirt tracks. Despite the merciless pounding the car received, the only reported fault was a broken temperature sensor on a catalyst, testament to the 550s mechanical ruggedness. Using four valves per cylinder instead of the increasingly common Ferrari five, the Maranello is a surprisingly uncomplicated beast. Chicago Motor Cars, Chicago Motor Cars, Chicago Motor Cars, Chicago Motor Cars, Chicago Motor Cars, Chicago Motor Cars, Chicago Motor Cars, Chicago Motor Cars, Chicago Motor Cars, Chicago Motor Cars, Chicago Motor Cars, Chicago Motor Cars.chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars chicago motor cars www.chicagomotorcars.com
The Ferrari factory in Maranello Italy We take a rare tour inside the Ferrari factory and learn the unique way they make each car a piece of art.
Ferrari f1 96 vs Ferrari 550 Maranello vs Fiat Bravo 2.0 Hgt Carrera el la que se enfrentan estos coches pilotados por Michael Schumacher, Eddie Irvine, Nicolas Larini en la que en la salida se da un tiempo entre coche y coche saliendo el ultimo el ferrari de Michael y llegando eel primero en el ultimo instante de carrera
"Terminator" Vs Range Rover - TerraMax - Top Gear - Series 19 - BBC James May assesses the new Range Rover at Nevada's Automotive Test Center for the ultimate challenge against an autonomous military machine - the Terminator of all terrain vehicles; The TerraMax. Subscribe for more awesome Top Gear videos: http://www.youtube.com/subscription_center?add_user=Topgear Top Gear YouTube channel: http://www.youtube.com/topgear TopGear.com website: http://www.topgear.com Top Gear Facebook: http://www.facebook.com/topgear Top Gear Twitter: http://twitter.com/BBC_topgear This is a channel from BBC Worldwide who help fund new BBC programmes.
Maria in 1000hp Twin Turbo Lamborghini Superleggera Maria in 1000hp Twin turbo Lamborghini Superleggera http://www.facebook.com/NueVuePhotography
FERRARI 458 ITALIA SPIDER cam on board Maranello 21/10/2012
340 km/h en Ferrari 430 Scuderia NovitecRosso (Option Auto) F430 Scuderia Bi Compresseur de 717 ch ! - ON ROAD SPEEDO / NOT A SINGLE Dyno TEST ON OPTION AUTO VIDEOS - www.optionauto.com -
2x Lamborghini Gallardo Racing on Autobahn!! - 1080p HD FB: http://www.facebook.com/Jorrie2YT & http://www.facebook.com/jorrik.aben Yesterday I had a great day during an ''Autobahn Run''. We had a few nice cars together and I participated with my GTI. In this first video you see two black Lamborghini Gallardos, one is totally stock, but the other has a Tubi Exhaust and Testpipes. Thanks to that modification, this Gallardo has a very loud and high sound! I hope you like this video, stay tuned for more Autobahn action!
Eriksson's Ferrari Enzo This tuned black Enzo belonged to Stefan Eriksson who was crusing Beverly Hills just weeks before he totaled his other Enzo -- a red one -- doing well over 150 mph on Pacific Coast Highway in Malibu.
Ferrari F430 Spider test drive in Maranello. 25.08.2011, Maranello, Italy.
Ferrari em Maranello - 1 - Test Drive F430 Spider Dirigindo uma F430 Spider nas ruas apertadas de Maranello - Warm Up
1998 Ferrari 550 Maranello V12 FOR SALE HD High Def WWW.FLEMINGSULTIMATEGARAGE.COM Only 18K original serviced miles, 5.5 liter hand built V-12 @ @420hp, 6-speed close ratio transaxle, mirror finish Giallo Modena (Fly yellow) paint, tan + black full leather interior w/ dual power seats, 18 factory alloys w/ Pirellis, keyless entry/alarm, Tubi polished Exhaust, original $204K MSRP, stunning! 301.816.1000
Ferrari 550 Maranello vs BMW M3 Coupe 6-speed: MBOARD.com Ferrari 550 Maranello vs BMW M3 Coupe 6-speed: MBOARD.com http://www.m5board.com/vbulletin/e90-m3-sedan-e92-m3-coupe-e93-m3-convertib le-e46-m3-e36-m3-z4-m-z4-m-coupe/138882-bmw-m3-coupe-6-speed-vs-ferrrari-55 0-maranello.html#post1588175
HD: Jaguar XFR vs Ferrari 550 Maranello: GTBOARD.com HD: Jaguar XFR vs Ferrari 550 Maranello: http://www.m5board.com/vbulletin/e60-m5-e61-m5-touring-discussion/137368-su percar-shootout-2009-may-gtboard-com-lp640-lp560-4-m5-touring-m6-porsche-99 7-gt2-jaguar-xfr-e39-m5-ess-Supercharger-more.html
Ferrari 550 vs. Lamborghini Murcielago Ferrari 550 vs. Lamborghini Murcielago Drag Race
Which car is faster?
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1970 Chevrolet Chevelle SS: 6.250 @ 227.800 Tom Dwyer, Engine: 526 KB Olds, Supercharger: PSI-C Tires: Hoosier
1970 Chevrolet Chevelle : 7.071 @ 200.620 Doug Holmes, Engine: 604 Big Block Chevy, Turbos: twin 88 mm thumpers Tires: Hoosier 17 x 34.5 x 16
1971 Chevrolet Chevelle Doorslammer: 7.869 @ 172.540 Tom Dwyer, Engine: 454, Supercharger: 8-71 Littlefield Tires: Hoosier
1970 Chevrolet Chevelle SS: 8.210 @ 146.340 Justin Mexia, Engine: 454, Supercharger: weiand 8-71 Tires: Mikey Thompson ET slicks
1968 Chevrolet Chevelle : 8.730 @ 158.000 Joe, Engine: 540 BBC, Tires: M/T 29.5x10.5
1968 Chevrolet Chevelle : 8.954 @ 154.000 Dave Hodgman, Engine: 540ci, Supercharger: NO Turbos: NO Tires: 30x13.5x15
1967 Chevrolet Chevelle Malibu: 9.020 @ 147.360 John Wilson, Engine: 440 sbc, Tires: Hoosier
1997 Mclaren F1 GTR: 9.031 @ 149.200 Steve Pearce, Engine: V12,
1970 Chevrolet Chevelle SS: 9.039 @ 148.100 Ray DeAngelis, Engine: 540, Tires: 16X32 goodyear
1976 Chevrolet Chevelle luv: 9.110 @ 146.360 Glen Young, Engine: 400ci, Supercharger: n Turbos: n
1971 Chevrolet Chevelle 496: 9.363 @ 139.770 jas kooner, Engine: 496, Supercharger: none Turbos: none Tires: 29.5 X 10.5
1964 Chevrolet Chevelle SS: 9.400 @ 139.000 2 O'Clock, Engine: 555, Tires: goodyear
1967 Chevrolet Chevelle Super Sport #138: 9.421 @ 141.090 2 O'Clock Tommy, Engine: 555, Tires: goofyear
1967 Chevrolet Chevelle Malibu: 9.440 @ 138.670 John Wilson, Engine: 496-BBC, Tires: Hoosier
1969 Chevrolet Chevelle SS: 9.484 @ 140.680 Armando DOnofrio, Engine: 555 JA Performance, Supercharger: - Turbos: - Tires: Hoosier Radial Slicks 30x10.5x15
1964 Chevrolet Chevelle ss: 9.610 @ 138.000 Tommy Whatley, Engine: bbc 555, Tires: 15/15/32
1971 Chevrolet Chevelle SS 454: 9.655 @ 145.650 Dmorgan, Engine: 632, Tires: Hoosier
1969 Chevrolet Chevelle ss: 9.690 @ 137.730 Nick Maloley, Engine: 540, Tires: GoodYear
1970 Chevrolet Chevelle SS: 9.700 @ 137.420 Killer Ken Iamele, Engine: 414 small block, Supercharger: No Turbos: No Tires: Hoosiers
1965 Chevrolet Chevelle : 9.721 @ 139.470 john skavdal, Engine: 454, Tires: 31-16,5 - 15
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Written on: 03/04/2013
I recently ordered curtains from Tuiss order no T2181945WB and at the same time I ordered a blind for my mother order no T2189028WB. In summary:
samples arrived vey quickly
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curtains are beautiful& perfect fit as is blind
communication from the time of placing the order was excellent - able to track order
I've now requested some more samples and will be placing another order for curtains.
Nothing to dislike.
Will definitely recommend & will use again. Thank you!
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Valentino might have some sweet dreams tonight, the new Desmosedici is finally on his taste.
It seems the sun starts to shine and the black clouds of doubt will scatter soon: "I shortened to half the gap that i had in Valencia" meaning, 0.7 of a second away from Lorenzo but only 0.2 away from Spies and Crutchlow.
How would you describe on short this day?
I am happy, this new bike has a very good base where we can start working from. We managed to improve on all aspects that last season kept us still. This is wonderful
Can you go more intro details with this?
I can sit better in the saddle, as a position, I can ride now as I want to and I have so much fun. Besides that, on braking and entering the corner we deal with such a huge step forward but also in changing direction faster which was another problem that we had.
What changed?
Everything. Still, this is a new born bike, we are at the very beginning with it but now we finally can work better with the tires and bring these at optimum temperature.
You didn't find any bad points?
We are still suffering when accelerating, there it is where we loose much of the time, the power management is to brutal.
It's an engine related problem?
I don't think so, our engine is very powerful, we must work on electronics because Honda for instance, manages to put all their horsepower on ground.
A big problem of GP 11 was that it didn't reacted to different settings, is it different with GP 12?
All is ok now, we tried little changes during the day and we constantly improved the times, an impossible thing to do some months ago.
How can you translate all these changes in terms of riding the bike?
I feel stronger and safer, i can finally brake as I like to and take my own lines, i made 40 laps today without a single error. Now everything is under control.
Did you expected for this?
I tell you this: before this test I could only dream about it being like this.
What do you expect now in the future?
I think that when you test a totally new bike, the good day you start to see it happen since morning and I tell you, this was such a good morning that happened. I don't have any objectives, now we need to work because until a victory to be possible we still miss some things and after all the three days will be over, we'll have a more clear image of the situation.
To see the real limits of the bike you must start lapping in range of 2' or 1'59" on this track.
You also tested out the new Bridgestone tires.
They work a little bit better than the old ones but I didn't noticed a big difference.
After his premature retirement at just 27 years old, it's rumored again that the Aussie could return with Ducati. Valentino Rossi gave an interview in which he also mentioned Stoner "If Stoner would have ridden the Ducati today, he wouldn't have finished higher than sixth place". I was thinking about a challenge, but of course this could be only in my de...
In a recent interview Nakamoto gave to GPone.com, the Honda - HRC Executive vice president - made an interesting affirmation. How did they came up in their minds to put this kind of question? Rumors behind the scenes again but I admit that any kind of a constructive rumors there are, I wish could be true and see Ducati on the level again. Read after the jump.
Luigi Dall'Igna is finally on Ducati's box and started the work for the 2014 season. Dall'Igna gave an interview in which speaks about the new company and about the way he wants to organize the things at Borgo Panigale, the future plans and the situation that found at Ducati.
Nothing new for Ducati at Valencia either, among the last MotoGP machines can only test your morale once again. "We are not among the first ones, as usual and the gap can only increase. All the others can take the correct lines, meaning a cooler tyres" Dovizioso said, more of this interview after the jump.
The former Supersport champion spoke to crash.net about his feelings in these last days with Tech3. Being in Moto GP since 2011, Cal spent all these years with only one team, Herve Poncharal gave him the trust and had a good hand regarding the brit's talent, now in front of a new challenge.
The last Desmo saddle available in Moto GP was given to Yonny Hernandez. At Borgo Panigale they decided to renew the trust in the Colombian rider and the 'Open' Desmosedici will be given to the young rider which proved to be a skilled rider and adapted very fast to the new environment and the GP machine.
The 31st of October 2013, brand new start for Ducati in World SBK. Both riders are new - Davide Giugliano and Chaz Davies and the championship partner changed also, after Alstare got out of the mix because of the most disastrous season ever in WSBK for Ducati, Feel Racing will be the new ally in 2014.
It's official, the ex Ducati rider will leave Althea to rejoin the new Ducati team at Jerez for testing on 30 and 31st of October. The 24 year old rider finished sixth in the World SBK 2013 standings, the first among the privateer riders finishing 2nd in two of the races in this season.
An anouncement was released about Spie's retirement. 'Ducati Motor Holding and Ben Spies announced today that the American will not be racing in 2014 after the parties reached agreement to resolve Ben's current contract with the Italian racing manufacturer,' reads a Saturday statement.
Starting from 2014 Chaz Davies and Sylvan Barrier will be the World SBK Ducati official riders. It's a bit odd, or at least this is how it looks to me, after Nicolo Canepa brought a 2nd place with Panigale in WSTK 1000 and a good result in Superpole @ Laguna Seca, the Italian was the first in my mind to have a seat in 2014.
There's a time when you must take though decisions, after a disastrous 2013 Carlos decided. The 2011 World SBK Champion will come in visit and say goodbye on Sunday at Jerez where the SBK round will take part this weekend, goodbye from the fans and from his team.
The MotoGP squad is ready and final, but what about the World SBK project? After Gigi Dall'Igna joined the 'red' family as a Ducati Corse manager the Borgo Panigale manufacturer made a strong stand towards the future, now, the SBK plan must also find a resolution regarding the fac that Alstare deal is on the edge.
Ducati starts to confirm the potential, Magny Cours wasn't the best weekend but showed that Panigale can. After the Laguna Seca round where Nicolo Canepa managed to finish with a +0.184 difference in Superpole, Ducati showed that all the tests and the new parts brought in the mix are starting to pay off the efforts and this week end, in France, Pirro brought the 1...
After a first difficult year in World SBK alongside Ducati, Francis Batta gives up. 2013 started in the best way possible, an incredible best lap time in Australia, but continued in the worse possible manner, severe injury for Checa and a 1199 Panigale struggling in the mid positions of championship.
As Carlos Checa finished his season by injuring himself at Istambul Ducati had to find a solution. After Laguna Seca, where Nicolo Canepa brought the 1199 Panigale in top 4 in Superpole and again in WUP, Michele Pirro was chosen to continue the WSBK adventure in these last rounds of the 2013 season.
2013 is one year to forget for Ducati for both competitions, Moto Gp and World SBK. Gpone took an interview to Ernesto Marinelli, the WSBK project manager, and here there are some answers after this horror 2013 which doesn't seem to become much better with just three rounds before the season's end.
The results of the Aragón Grand Prix, the fourteenth round of the MotoGP World Championship, were less than satisfying for the Ducati Team. Both Andrea Dovizioso and Nicky Hayden were able to improve slightly on their grid positions (on the third and fourth row, thanks to ninth and eleventh place finishes in qualifying), but at the finish line, they had to settle ...
Carlos Checa will miss the rest of the races this season. After the Istambul incident in the free practice day, the doctors found the problem was more serious and the Spaniard has a hip fracture and because of this, the Ducati rider won't be able to ride this season anymore.
Is not yet official, but the Kentucky Kid will ride an Aprilia in 2014. Aleix Espargaro will leave the team next season to ride a full Moto GP bike for Team Forward and by this, and by this a place was freed under the ART colors.
If you missed Ben's interview from motogp.com, here it is. Lots of rumors out there rearding Ben's future, many said the Pramac team will no longer keep him for 2014, the twitter messages that the American wrote were also not helping out the situation, som here it is what the rider had to say about all these and also about the time he will jump on the saddle.


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The event includes a 1.5-mile fun run around the Blue Dome District (streets will be closed for safety,) costume contests, candy and prizes. New for 2012 is a dog category – runners can bring their dogs to run in the fun run, and there will be a prize for the best K9 costume.
This is a great way to burn off some Halloween candy energy while also incorporating running, walking and fitness into a holiday tradition. The run is scheduled this year for Oct. 30, so it doesn't interfere with typical trick-or-treating.
HallowZOOeen 2012 will showcase some treasured treats with Burger King Royal Castle and American Airlines "Pirate Island". Burger King's Royal Castle summons all Green Country royalty to join the Tulsa Zoo for a splendid to-do where your princess (or prince charming!) can be amused with royal court activities, including archery with nerf guns!
Perhaps a sailor's life suits thee more? Then chart yer course for American Airlines "Pirate Island" for a swashbuckling adventure with the American Airlines pirates. Every "buoy" and "ghoul" will enjoy the one-of-a-kind pirate ship, where seaworthy sprouts can take the helm.
No HallowZOOeen is complete without a tour through the American Airlines Haunted House or a spin on the Haunted Train or Cross-Eyed Carousel. Kids of all ages will enjoy the CommunityCare "Pumpkin Patch Playroom" with a hay maze, pumpkin decorating and carnival games.
Of course the Tulsa Zoo hasn't forgotten about the CANDY! Corporate-sponsored Goblin Stops line the zoo to provide a safe trick-or-treating environment where your kiddos can fill up their goodie bags - and with more than 3 tons of candy, there will be plenty of treats!
Tulsa Zoo's HallowZOOeen will take place Oct. 27-31. Tickets can be purchased online or at the Tulsa Zoo daily from 9a.m.-5p.m.
The cost for admission is $8 for the general public and $7 for Tulsa Zoo Friends members. Children 1 and under are free if not trick-or-treating.
WOODLAND HILLS MALL TO HOST SAFE TRICK-OR-TREATINGKids are invited to enjoy fun and goodies in a safe environment! WHAT: Ghosts, goblins and ghouls, oh my! Your favorite little black cats and spooky bats are invited to Woodland Hills Mall on Tuesday, October 30 from 6:00 - 8:00PM for a fun and safe night at Mall-o-ween Halloween celebration! For children 12 years old and younger, throw on your best costume and come scour the mall and various retailers for candy, all in a safe and climate-controlled environment. Parents can visit with family friendly businesses and learn more about Simon Malls' Kidgits Club program while your kiddos trick-or-treat. The event will be presented by The Hub TV Network, a destination for kids and their families. The Hub will provide fun activities for attendees, giveaways and trick-or-treat bags (while supplies last). Additionally, during the event, little ones can view some of the network's fun, kid-friendly, spooky-themed episodes of shows including "My Little Pony Friendship is Magic" and "Transformers Rescue Bots." This will be a spooktacular Halloween that you and your kiddos won't want to miss!COST: FREE and open to the public. WHEN: Tuesday, October 30 6:00 p.m. - 8:00 p.m. WHERE: Woodland Hills Mall- Upper and lower levels of mall7021 S Memorial Dr.Tulsa, OK 74133
For the third year, officers from the Tulsa Police Department's Riverside Division will host the Haunted Halloween Precinct on Monday, Oct. 29 from 5 to 7 p.m.Families can look at police equipment including, a helicopter, Saracen and patrol cars, and visit with the officers who use them. Children 12 and under can play games and receive safe treats at the festivities.Sponsors for the event are QuikTrip Corporation, Target and Tulsa Federal Credit Union. The Riverside Division is located at 7515 Riverside Drive.
Mall-O-Ween at Tulsa Promenade Mall, October 31st 6pm-8pmChildren asked to bring canned goods for food drive benefiting the Community Food Bank of Eastern Oklahoma Tulsa, OK (October 15, 2012)-----Promenade Mall, located at 4107 S. Yale Avenue, invites the public to their annual Mall-O-Ween event Wednesday, October 31st from 6:00 pm to 8:00 pm. Children and their parents are invited for mall wide trick or treating with participating retailers and a canned food drive for the Community Food Bank of Eastern Oklahoma. In addition 106.9 KHITS radio station will sponsor a children's costume contest at 7:00 pm in Center Court. Prizes will be awarded in several categories including · Best Baby Costume (birth to one yr)· Cutest Costume· Funniest Costume· Scariest Costume· Most Original Costume· Best of Show "We are excited to be promoting a community canned food drive. We ask all trick-or-treaters to bring at least one canned food with them on Halloween. The food will be donated to the Community Food Bank of Eastern Oklahoma," said Sheila Cooper, Mall Manager for Tulsa Promenade Mall. "Promenade Mall provides a comfortable environment for families to enjoy a fun Halloween. Our participating retailers will be handing out treats to all princesses, fairies and ghosts descending upon the mall on Halloween." Halloween has definitely become more than just candy and treats. According to the National Retail Federation's (NRF) 2012 Halloween Consumer Spending Survey Americans are expected to spend $2.87 billion on Halloween costumes this year, spending over $1.1 billion on children's costumes, $1.4 billion on adult costumes, and $370 million on pet costumes. The average person will spend $79.82 on Halloween candy, costumes and décor, bringing total spending to $8 billion - the most in the survey's ten-year history. Several stores in Promenade Mall are catering to the Halloween shopper. Shoppers to the mall can visit Halloween Express, a store dedicated to Halloween costumes and decorations. This store is located on the lower level across from Victoria's Secret. In addition several stores have Halloween accessories as well including Claire's Accessories, Hot Topic, and Romancing the Stone.
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English-German translation for "extradition treaty"
"extradition treaty" German translation
extradition treaty
In addition to considerations based on the extradition treaty with the USA, account must also be taken of general international law and international public policy.
States Parties undertake to include such offences as extraditable offences in every extradition treaty to be subsequently concluded between them.
States Parties undertake to include such offences as extraditable offences in every extradition treaty to be concluded between them.
The US Embassy in Berne submitted the formal extradition request to the FOJ on 22 October 2009, within the deadline of 40 days stipulated under the bilateral extradition treaty.
Similar translations
Similar translations for "extradition treaty" in German
Usage examples
Usage examples for "extradition treaty" in German
Do the existing extradition treaties not offer sufficient scope in this area?
The IMAA also allows extradition without a treaty obligation, however.
The extradition treaties do not lay down any clear rules on priority.
Old extradition treaties contain a list of offences for which provision for extradition is made.
For some Member States that already have a bilateral extradition treaty with the US this is nothing new.
First as regards the extradition treaty.
No, we cannot have Interpol; we cannot have normal extradition treaties between Member States!
Switzerland has concluded bilateral or multilateral extradition treaties with most European and many non-European States.
Member States can conclude a non-extradition treaty with the United States with representatives dispatched by the government in Washington.
If this leads to the opening of a criminal case - as is foreseeable - Pinochet would even have to be extradited to Spain under an existing extradition treaty.
But for Member States without a bilateral extradition treaty and for Member States that operate a so-called positive list there will be a sweeping change.
The Amsterdam Treaty represents progress along the road to equal opportunities.
I believe that article K.3 of the Treaty on European Union was perfectly sound.
That is covered by a special procedure laid down in Article 141 of the EC Treaty.
There is a suggestion that sport should be included in Article 128 of the Treaty.
Protocol amending the Treaty establishing the European Atomic Energy Community
The requirement of unanimity in the case of treaty changes must be maintained.
There are several concrete proposals on security in the Constitutional Treaty.
This regulation goes further than the Treaty of Rotterdam on a number of points.
- the Palestinian side does not want to conclude a permanent treaty with Israel.
Suggest new English to German translation
Do you know of specific regional German expressions? Perhaps you have a great German translation for a particular English phrase? If you do then you can make your own addition to the English-German dictionary here.
Latest word suggestions by users: imprint obligation, obligation to provide an imprint, obligation to provide a masthead, overzealousness, officiousness
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extracting · extraction · extractions · extractor · extracts · extracurricular · extradited · extradites · extraditing · extradition · extradition-treaty · extrajudicial · extramarital · extramedullary · extramural · extraneous · extraneousness · extranet · extraordinarily · extraordinary · extrapolated
More translations in the bab.la English-Danish dictionary.


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HAMPTON - Three people standing on a sidewalk were injured Tuesday afternoon after they were struck by a car that went off Ocean Boulevard and stopped only after crashing into concrete columns.
The pedestrians – two men and a woman – were hit around 2:20 p.m. in front of the Hampton Beach Casino Ballroom near the intersection of D Street and Ocean Boulevard. They were rushed to Portsmouth Regional Hospital, but their injuries were not believed to be life-threatening, Hampton Deputy Police Chief Rich Sawyer said.
The car was traveling north on Ocean Boulevard when it sideswiped a truck parked in a loading zone, rear-ended another vehicle sitting in traffic, and then continued north and eventually hit the pedestrians before slamming into two columns attached to the Casino Ballroom, Sawyer said.
One of the male pedestrians suffered a fractured leg while the second man received a head injury and neck laceration. The woman suffered minor leg abrasions, Sawyer said.
The 49-year-old male driver and his female passenger were transported to Exeter Hospital with non-life threatening injuries.
The cause of the crash remains under investigation.
"We're not quite sure what occurred. We haven't really had a chance to talk to him to get his perspective on what happened. We don't know if it was something medical, we're not sure," Sawyer said.
The occupants of the other vehicles involved weren't hurt.
Police have not yet released the names of those involved in the accident.
Sawyer was in the area at the time of the accident.
"We just heard people screaming and yelling and running," he said. "This could have been a lot more serious. We're just lucky that there weren't more pedestrians."
Workers at The Whale's Tale 2 restaurant said a mother, young child in a stroller, and a grandmother who were eating pizza on the sidewalk just seconds before the accident.
Sera Fowler, a restaurant manager, said the mother had just taken her child out of the stroller and they ran when they saw the car coming. They weren't hurt, but the stroller was crushed, she said.
"They were just hysterical," she said of the family, who sat inside the restaurant located in front of the accident scene to fill out an incident report.
Restaurant co-owner Kevin Jacques said many of the customers inside the restaurant were upset.
"We heard it and we felt it," he said.
Devin Dorin, who works for Casino Fast Food, said he saw the car "drifting" off Ocean Boulevard and then hit the column. "I saw smoke and then everybody started running around," he said.
Tom Pawley of Cell Accessories said he heard the crash and people screaming. He said he saw people on the ground when he came out of the store to see what happened.
Pawley said he was surprised that more people weren't hurt.
"It was packed. There were literally hundreds out here," he said.


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He's working from home today, so I just went over to his computer and commenced with the textual healing. I wasn't made to debase myself! What an incredible change of pace.
We're past the point where video game consoles are just pieces of hardware for games. The PlayStation 4 may be less expensive than the Xbox One and more focused on games, but it's still filled with features that betray Sony's… Read More
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text:
Global Soldier Modernisation Market Assessment
- Language: English
- 160 Pages
- Published: May 2012
- Region: Global
As of June 15, 2011, Standard & Poor's Ratings Services had received notification from collateralized debt obligation (CDO) trustees of 442 events of default (EODs) on cash flow and hybrid mezzanine structured finance (SF) CDOs of asset-backed securities (ABS), high-grade SF CDOs of ABS, CDOs of commercial mortgage-backed securities (CMBS), and CDO of SF CDO transactions. Our list includes global cash flow and hybrid CDO transactions originated since 2001 that have experienced an EOD as a result of credit deterioration of recent-vintage ABS, either by directly holding notes from the ABS transactions or by holding notes from other CDO transactions that are in turn collateralized by ABS. The 442 transactions listed below for which we have received EOD notifications to...
Standard and Poors RatingsXpress Credit Research provides in-depth coverage of international corporates, financial institutions, insurance companies, utilities, sovereigns and structured finance programs. RatingsXpress Credit Research lets users determine the credit rating of holdings and identify key factors underlying an issuer's creditworthiness, distinguishes the different risk exposures for


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Item specifics
- Place of Origin:
- Guangdong China (Mainland)
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Classic Lanes
The Classic Lanes Bowling Alley has been in the community for at least 25 years. Here you can bowl for $3.95 a game and rent shoes for $4.
3201 E End Ave, South Chicago Heights, IL 60411
(708) 754-4210
- Accessible: yes
Posts awaiting your approval 0
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- Chicago Heights Patch Editor
- Ryan Fitzpatrick
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Copyright ©2013 Patch. All rights reserved.


text:
« Reactions To The Michael Bourn Signing | Main | Pirates, Brandon Inge Close To Deal »
Quick Hits: O's, Verlander, Royals, Crawford
Justin Verlander repeated today that he wants to stay with the Tigers beyond 2014, when his current contract expires. "I love Detroit," he said, according to John Lowe of the Detroit Free Press. "I feel like I'm a kindred part of that town. I grew up in front of these fans and earned my way into their hearts." Verlander said he's not yet sure whether he and his representatives would be willing to negotiate during the season. Here are some more links from around MLB...
- Grantland's Jonah Keri interviewed Orioles GM Dan Duquette about the success of his team in 2012 and Duquette's outlook on 2013. Duquette feels that both Dylan Bundy and Kevin Gausman will be ready to make significant impacts on the club at some point in 2013.
- ESPN's Jayson Stark presents an in-depth look at the top stories to watch in each league during Spring Training this year. Stark's piece also includes breakdowns of the best and worst free agent signings, trades and more, according to a group of 16 baseball scouts and executives.
- Dave Cameron of FanGraphs lists the ten worst moves of the offseason with the caveat that "the worst moves aren't as bad as bad as they used to be." The Royals make three appearances on the list after trading Wil Myers to obtain James Shields, acquiring Ervin Santana and signing Jeremy Guthrie for three years.
- Meanwhile, in another piece for Grantland, Keri examines the worst contracts in MLB starting with Alex Rodriguez of the Yankees and Carl Crawford of the Dodgers.
- Agents at Hendricks Sports have filed an MLBPA grievance against three MLB agents who left the firm for Excel Sports Management and took players with them, Liz Mullen of the Sports Business Journal reports (on Twitter). Be sure to use MLBTR's Agency Database for your agency-related inquiries.
Full Story |
Comments (0) | Categories:
Alex Rodriguez
| Baltimore Orioles
| Carl Crawford
| Detroit Tigers
| Dylan Bundy
| Justin Verlander
| Kansas City Royals
| Kevin Gausman
| Los Angeles Dodgers
| New York Yankees
White Sox Sign Felipe Paulino
Roy Halladay To Retire
Marlins To Sign Garrett Jones
Astros Sign Chad Qualls
Yankees To Sign Carlos Beltran
Red Sox To Re-Sign Mike Napoli
Nationals To Sign Nate McLouth
Rangers To Sign J.P. Arencibia
Astros Sign Scott Feldman
Mets Sign Curtis Granderson
Mariners To Sign Robinson Cano
Yankees To Re-Sign Hiroki Kuroda
Orioles To Sign Francisco Peguero
Orioles To Sign Ryan Webb
Marlins To Sign Rafael Furcal
White Sox To Sign Ronald Belisario
Dodgers Re-Sign Brian Wilson
Red Sox To Sign Edward Mujica
Royals Acquire Norichika Aoki
Yankees To Sign Kelly Johnson
Cubs Sign Wesley Wright
White Sox Re-Sign Paul Konerko
Rockies To Sign Justin Morneau
Yankees Agree To Sign Jacoby Ellsbury
Marlins Sign Jarrod Saltalamacchia
Astros Acquire Dexter Fowler
Padres, A's Swap Seth Smith For Luke Gregerson
A's Acquire Craig Gentry, Josh Lindblom
Rays Extend Ryan Hanigan
2014 Top 50 Free Agents With Predictions
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Projected Arbitration Salaries For 2014
Free Agent Prediction Contest Leaderboard
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D-Backs To Acquire Trumbo In Three-Team Trade With Angels, White Sox
Phillies Seeking Two Or Three Young Players For Domonic Brown
Seven Teams Interested In Logan Morrison
Twins, Mets, Reds Pursuing Arroyo
New York Notes: Reynolds, Logan, Gardner, Mets
Stephen Drew Rumors: Tuesday
Youkilis Drawing Interest, Prefers West Coast
Rockies, Reds Talked Sean Marshall
At Least Seven Teams Interested In Eric Chavez
Tigers, Rajai Davis Agree To Two-Year Deal
Braves In Talks With Eric O'Flaherty
D-Backs, ChiSox, Angels Optimistic About Three-Team Deal
AL East Notes: Moreland, Joyce, Ibanez, Floyd, Yanks
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text:
Orleans is headed back to the Cape Cod Baseball League championship for the first time since 2005 with a dramatic victory over Chatham. In the West, Bourne broke open a scoreless game in the fourth inning and never looked back en route to a victory over Cotuit, which forces a game three.
Orleans 6, Chatham 5
The Firebirds were the hottest team in the league at the end of the regular season, and they're not cooling off in the postseason.
After watching a hard-charging Chatham team rally from a three-run deficit to take the lead, Orleans immediately scored two runs to tie it then scored what proved to be the winning run in the eighth. The Firebirds are in the finals for the first time since 2005, when they also beat Chatham in the Eastern Division championship series.
This Orleans team won 11 of its last 13 in the regular season, and has now won four of five in the playoffs. That's 15 of 18 overall, and the only non-wins were a two-run loss, a one-run loss and a tie.
The Anglers still figured to be a very tough out. They had a "team of destiny" kind of feel around them all season, and even after a 7-3 loss in game one, I don't think anyone would have been surprised to see a game three.
J.D. Davis (Cal State Fullerton) gave Chatham a 1-0 lead with his second home run of the post-season, a controversial one that was apparently caught by Geoff DeGroot (UMass Lowell) as he flipped over the center field fence. Orleans answered and went up 3-1 before Chatham scored four in the sixth. Connor Joe's two-run homer gave the Anglers the lead.
With the game in the hands of the strong Chatham bullpen, the Anglers seemed to be in good shape, but Orleans quickly answered. In the bottom of the sixth, DeGroot, the ninth-place hitter, doubled and then stole third. He scored on a wild pitch. Austin Davidson (Pepperdine) then brought home the tying run on a groundout.
After a scoreless seventh for both teams, Orleans took the lead in the eighth. Ross Kivett (Kansas State) singled, took second on a balk and third on a passed ball, and scored on a sac fly by Davidson. It was the sixth RBI of the series for Davidson.
In the ninth, Orleans gave the ball to closer Matt Troupe (Arizona) as he tried to shut down a Chatham team that won five games in the ninth inning or later this season.
There was no magic this time. Troupe gave up a one-out single but struck out the other three batters to give Orleans a spot in the championship.
For Chatham, it was a tough ending to a terrific season, the franchise's best in a long time. I think they'll be remembered much like the 2005 Chatham team that lost to Orleans – a talented and special group, regardless of where they finished.
Chatham's loss also means it's now been six years since the team with the best record in the regular season went on to win the championship. The 2007 Y-D Red Sox were the last ones.
Bourne 8, Cotuit 1
Cotuit starter Tommy Kister (The Masters College) no-hit the Braves for three-plus innings, but once they broke the seal, the floodgates soon followed.
The Braves got their first hit with two outs in the fourth when Jeff Gardner (Louisville) hit a line drive to center that Bradley Zimmer (San Francisco) couldn't come up with on a sliding try. Vinny Siena (Connecticut) followed with a solid base hit to score a run before Trent Gilbert (Arizona) doubled to score two.
Just like that, it was a 3-0 game, and the Braves never really looked back. They added two runs in the fifth and three in the sixth while keeping Cotuit's bats quiet the rest of the way.
Gilbert's two RBI led the offense, while Mason Robbins (Southern Miss) also drove in two. Clint Freeman (East Tennessee State) had an RBI triple, his second in as many games.
Bourne got a strong start from Christian Colletti (Connecticut), who was making just his second appearance. He struck out eight and allowed just three hits in four shutout innings. Michael Costello (Radford) got the win with three strong innings of relief. With the big lead, Will Cox (Mississippi State) and Jack English (Florida Gulf Coast) slammed the door.
Game three is set for 4 p.m. today in Cotuit. Ben Smith (Coastal Carolina), who's been a steady performer all summer, is slated to start for Cotuit. Bourne's starter is TBA.


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"It's hard to flip it, but it's the playoffs. You're always flipping the switch come playoff time."
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Doug Swink


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1-10 of 90 two bedroom houses for sale found
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Brow Lift
Recent comments
Posted to
Brow Lift, Upper & Lower Eyelids... Couldn't Be Happier! - Phoenix, AZ
on 24 Jun 2013
This is a great review; thank you. I had an endoscopic brow lift and blepharoplasty 5 days ago, and so far, my experience sounds the same as yours. Lots of nausea and pain the first night, but a surprisingly quick recovery afterwards. My only concern now is my forehead feels very tight; it doesn't look stretched but it feels so tight I don't want to frown or raise my eyebrows. Did you feel that way? BTW, your results are fantastic!


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XWiki is a WikiWiki clone written in Java that supports many popular features of other Wikis like the Wiki syntax, version control, attachments, security, and searching, but also many advanced features like templates, database and dynamic development using scripting languages (Velocity, Groovy, Ruby, Python, PHP, and more), an extension system and skinability, J2EE scalability, an XML/RPC remote API, statistics, RSS feeds, PDF exporting, WYSIWYG editing, an Office viewer and importer, and a lot more.
phpMyFAQ is a multilingual, completely database-driven FAQ system. It support various database systems and it also offers a content management system with a WYSIWYG editor, an image manager, flexible multi-group and multi-user support, a news system, user tracking, language modules, templates, PDF support, a backup system, Active Directory support, and an easy to use installation script.
Tiki Wiki CMS Groupware is a full-featured, Web-based, multilingual, tightly integrated, all-in-one wiki, CMS, and groupware. Tiki can be used to create all kinds of Web applications, sites, portals, knowledge bases, intranets, and extranets. Tiki offers a very large number of features "out-of-the-box". It is highly configurable and modular. All features are optional and administered via a Web-based interface. Major features include a robust wiki engine, news articles, discussion forums, newsletters, blogs, file and image galleries, bug and issue trackers, a link directory, polls/surveys and quizzes, FAQs, a banner management system, calendar, maps, mobile access, RSS feeds, a category system, tags, an advanced themeing engine (Smarty), spreadsheet, live support, shoutbox, inter-user messaging, menu generator, advanced permission system for users and groups, internal search engine, external authentication support, and more. It was formerly named TikiWiki.
WebIssues is a multi-platform system for issue tracking and team collaboration. It can be used to store, share, and track issues with various attributes, description, comments, and file attachments. It is easy to install and use but has many capabilities and is highly customizable. Desktop and Web clients are provided.
PeaZip is a cross-platform file archiver utility that provides a unified portable GUI for many open source technologies like 7-Zip, FreeArc, PAQ, UPX, etc. Creates 7Z, ARC, BZ2, GZ, *PAQ, PEA, QUAD/BALZ, TAR, UPX, WIM, XZ, and ZIP files. It extracts more than 150 archive types: ACE, ARJ, CAB, DMG, ISO, LHA, RAR, UDF, ZIPX, and more. Features of PeaZip include extracting, creating, and converting multiple archives at once, creating self-extracting archives, split/join files, strong encryption with two factor authentication, an encrypted password manager, secure deletion, find duplicate files, calculate hashes, and export job definitions as scripts.
ZK is a highly productive Java framework for building amazing enterprise Web and mobile applications. With event-driven components and an XUL-based markup language, developing is as simple as programming desktops and authoring HTML. Scripting languages include but are not limited to Java, JavaScript, Ruby, and Groovy.
Cerb is a fast and flexible Web-based platform for business collaboration and automation. It helps you remember anything about anyone, collaborate from anywhere, deftly reply to a flood of email, automate, stay informed, capture feedback, track time, flag opportunities, manage tasks, share expert knowledge, and otherwise execute efficiently.
A set of utility classes that can be used for Desktop application development.


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Would appreciate hearing personal reports on the new "SRH" in Cuba.I would like to hear,the good and the bad,not, the usual AI bashing.Thanks........
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Its a beautiful place with beautiful people - you'll love it!
Report with pictures of my visit in October 04 here:
For more up-to-date info go to www.tropictravelonline.com there is an active discussion about this place.
Martin -
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I had clients just return from there and they loved it. They said the service was great, and the grounds amazing. They didn't have any complaints. From the pictures, it does not look as bright and colourful as some other Sandals properties (which is a good thing IMO)
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I Wife Sharing
My Wife Sharing Experience
About 3 years ago I started working for a new company. I became friends with a guy there, and over the course of about a year as couples, we got to be really good friends. His wife and my wife liked each other and as guys we liked the same sports and things. There were nights I would stop by their place by myself and have a beer with Jim and just kill a couple of hours before going home from work. About a year ago I stopped by on the way home and had a beer with Jim and we just sat on his patio and talked. Our conversations usually ranged from sports to cars or whatevr come to mind. As we were talking he said he would like to ask me something, and not to offend me or anything, but it was kinda personal. I said sure, whats on your mind. He asked me what I thought about open marriages. Well I was a bit set back, I didn't think about him asking anything like that. I kinda gave a general answer, you know, what do you say?, so I said, I dont know, I suppose its ok, as long as its safe, private and so on. And then I had to ask what brought that up. And I about fell out of my chair when he told me what they were thinking. He actually asked me if I would consider having sex with his wife, saying she was all for it and that its something they had discussed for a long time. He told me they never did it, would be a first for them. I told him, wow..what a question to ask someone out of the blue, I dont know what to say. He said I know, and he promised total secrecy and privacy, his only request was to be there, in the room, an observer. I told him we were good friends, I don't want to screw that up. I asked, are you sure your wife really wants this?, are you kidding me or something? He assured me it was no joke, we had walked on itno the kitchen as we were chatting all this time, to get another beer. His wife Linda had walked in from the livingroom. He told her he had asked me, he kinda looked at me for an answer, and he turned and shut the door. I guess I must have looked blank or something, he said..Mike, its a first for us too. It took alot of time for me to ask. I couldnt keep from looking at her, finially I said ok, but whatever, were still friends, and cant tell anyone, I mean anyone, including my wife, because that would be the end for me. So with that I followed their lead, they took me to their bedroom and Jim sat in a chair opposite the bed and watched as Linda and I had sex. She gave me oral, I licked her a bit, and then straight to it. She kept moaning and telling me how good it felt, and that got me going that much more. I cummed so hard as I was thinking of my ***** spurting into my friends wife, and how she was wanting it so bad. Just wanted to share this. I am always interested in hearing others encounters as well. firstname.lastname@example.com can join Experience Project now to respond to this story!
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great story. i want my wife to **** other guys. i would like to find her several regular **** buddies.i would like to call up a freind and say hey want to meet us for dinner and drinks knowing he was going to be ******* my wife in a couple of hours.
Gotta tell ya dude..you are now hooked on enjoying other's wives... I don't know of one thing that is more exciting that the first encounter with a wife.. Bill in Va.


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- November 14, 2012
- 10:08 a.m.
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Here's a link to the Tennessee petition to secede. Names of those who signed the petition are at the bottom of the page.
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Publications (10)40.86 Total impact
- [show abstract] [hide abstract]
ABSTRACT: Human immunodeficiency virus type 1 (HIV-1) integrase (IN) functions in cells within the context of high molecular weight preintegration complexes (PICs). Lens epithelium-derived growth factor (LEDGF) transcriptional coactivator/p75 and hepatoma-derived growth factor related protein 2 (HRP2) tightly bind to HIV-1 IN and stimulate its integration activity in vitro. Here, we show that each recombinant host cell factor efficiently reconstitutes the in vitro activity of HIV-1 PICs disrupted for functional integration by pre-treatment with high concentrations of salt. Mutational analysis reveals that both the IN-binding and DNA-binding activities of LEDGF/p75 contribute to functional PIC reconstitution. We also investigate a role(s) for these proteins in HIV-1 infection by using short-interfering RNA. HIV-1 infection was essentially unaffected in HeLa-P4 cells depleted for LEDGF/p75, HRP2, or both proteins. We conclude that cells knocked-out for LEDGF/p75 and/or HRP2 will be useful genetic tools to address the roles of these host cell factors in HIV-1 replication.Virology 04/2006; 346(2):415-26. · 3.37 Impact Factor -
Article: HIV-1 incorporates and proteolytically processes human NDR1 and NDR2 serine-threonine kinases.
[show abstract] [hide abstract]
ABSTRACT: Mammalian genomes encode two related serine-threonine kinases, nuclear Dbf2 related (NDR)1 and NDR2, which are homologous to the Saccharomyces cerevisiae Dbf2 kinase. Recently, a yeast genetic screen implicated the Dbf2 kinase in Ty1 retrotransposition. Since several virion-incorporated kinases regulate the infectivity of human immunodeficiency virus type 1 (HIV-1), we speculated that the human NDR1 and NDR2 kinases might play a role in the HIV-1 life cycle. Here we show that the NDR1 and NDR2 kinases were incorporated into HIV-1 particles. Furthermore, NDR1 and NDR2 were cleaved by the HIV-1 protease (PR), both within virions and within producer cells. Truncation at the PR cleavage site altered NDR2 subcellular localization and inhibited NDR1 and NDR2 enzymatic activity. These studies identify two new virion-associated host cell enzymes and suggest a novel mechanism by which HIV-1 alters the intracellular environment of human cells.Virology 02/2005; 331(1):181-9. · 3.37 Impact Factor - [show abstract] [hide abstract]
ABSTRACT: Integrase has been implicated in human immunodeficiency virus type 1 (HIV-1) nuclear import. Integrase analyses, however, can be complicated by the pleiotropic nature of mutations: whereas class I mutants are integration defective, class II mutants display additional assembly and/or reverse transcription defects. We previously determined that HIV-1(V165A), originally reported as defective for nuclear import, was a class II mutant. Here we analyzed mutants containing changes in other putative nuclear localization signals, including (186)KRK(188)/(211)KELQKQITK(219) and Cys-130. Previous work established HIV-1(K186Q), HIV-1(Q214L/Q216L), and HIV-1(C130G) as replication defective, but phenotypic classification was unclear and nuclear import in nondividing cells was not addressed. Consistent with previous reports, most of the bipartite mutants studied here were replication defective. These mutants as well as HIV-1(V165A) synthesized reduced cDNA levels, but a normal fraction of mutant cDNA localized to dividing and nondividing cell nuclei. Somewhat surprisingly, recombinant class II mutant proteins were catalytically active, and class II Vpr-integrase fusion proteins efficiently complemented class I mutant virus. Since a class I Vpr-integrase mutant efficiently complemented class II mutant viruses under conditions in which class II Vpr-integrases failed to function, we conclude that classes I and II define two distinct complementation groups and suggest that class II mutants are primarily defective at a postnuclear entry step of HIV-1 replication. HIV-1(C130G) was also defective for reverse transcription, but Vpr-integrase(C130G) did not efficiently complement class I mutant HIV-1. Since HIV-1(C130A) grew like the wild type, we conclude that Cys-130 is not essential for replication and speculate that perturbation of integrase structure contributed to the pleiotropic HIV-1(C130G) phenotype.Journal of Virology 01/2005; 78(23):12735-46. · 5.08 Impact Factor - [show abstract] [hide abstract]
ABSTRACT: Human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) protein was recently identified as a binding partner for HIV-1 integrase (IN) in human cells. In this work, we used biochemical and bioinformatic approaches to define the domain organization of LEDGF/p75. Using limited proteolysis and deletion mutagenesis we show that the protein contains a pair of evolutionarily conserved domains, assuming about 35% of its sequence. Whereas the N-terminal PWWP domain had been recognized previously, the second domain is novel. It is comprised of approximately 80 amino acid residues and is both necessary and sufficient for binding to HIV-1 IN. Strikingly, the integrase binding domain (IBD) is not unique to LEDGF/p75, as a second human protein, hepatoma-derived growth factor-related protein 2 (HRP2), contains a homologous sequence. LEDGF/p75 and HRP2 IBDs avidly bound HIV-1 IN in an in vitro GST pull-down assay and each full-length protein potently stimulated HIV-1 IN activity in vitro. LEDGF/p75 and HRP2 are predicted to share a similar domain organization and have an evident evolutionary and likely functional relationship.Journal of Biological Chemistry 12/2004; 279(47):48883-92. · 4.65 Impact Factor - [show abstract] [hide abstract]
ABSTRACT: Integration, catalyzed by the viral integrase (IN) protein, is a crucial step in the life cycle of all retroviruses including human immunodeficiency virus type 1 (HIV-1). Although purified HIV-1 IN protein is sufficient to catalyze the DNA breakage and joining steps of integration in the absence of any other protein factor, a number of studies indicate that cellular proteins participate in the integration process in cells. These host cell proteins have been proposed to act through binding the pre-integrated viral cDNA substrate, by directly interacting with the IN protein, and/or by repairing the single-stranded DNA gaps that occur at viral/chromosomal DNA junctions during integration. In this paper we summarize the identification and potential roles of specific cell factors in HIV-1 integration. We also present experimental results of human cell proteins that coimmunoprecipitated with HIV-1 IN following its expression in HeLa cells and discuss these results in light of the previously-identified integration cofactors.Frontiers in Bioscience 10/2004; 9:3187-208. · 3.29 Impact Factor - [show abstract] [hide abstract]
ABSTRACT: Human NDR1 (nuclear Dbf2-related) is a widely expressed nuclear serine-threonine kinase that has been implicated in cell proliferation and/or tumor progression. Here we present molecular characterization of the human NDR2 serine-threonine kinase, which shares approximately 87% sequence identity with NDR1. NDR2 is expressed in most human tissues with the highest expression in the thymus. In contrast to NDR1, NDR2 is excluded from the nucleus and exhibits a punctate cytoplasmic distribution. The differential localization of NDR1 and NDR2 suggests that each kinase may serve distinct functions. Thus, to identify proteins that interact with NDR1 or NDR2, epitope-tagged kinases were immunoprecipitated from Jurkat T-cells. Two uncharacterized proteins that are homologous to the Saccharomyces cerevisiae kinase regulators Mob1 and Mob2 were identified. We demonstrate that NDR1 and NDR2 partially colocalize with human Mob2 in HeLa cells and confirm the NDR-Mob interactions in cell extracts. Interestingly, NDR1 and NDR2 form stable complexes with Mob2, and this association dramatically stimulates NDR1 and NDR2 catalytic activity. In summary, this work identifies a unique class of human kinase-activating subunits that may be functionally analagous to cyclins.Journal of Biological Chemistry 07/2004; 279(23):24444-51. · 4.65 Impact Factor - [show abstract] [hide abstract]
ABSTRACT: The ability of small interfering RNA (siRNA) to mediate gene-specific post-transcriptional silencing in mammalian cells will undoubtedly revolutionise functional genomics, as well as drug target identification and validation. Furthermore, there is widespread excitement that siRNA itself might prove useful in the clinical setting. For those wishing to develop siRNA as a therapeutic agent, the most difficult obstacle to overcome will be delivery. Recently, several breakthroughs have highlighted viruses as excellent vehicles for siRNA delivery. Retroviruses, the transgene-delivery vector of choice for many experimental gene therapy studies, have been engineered to deliver and stably express therapeutic siRNA within cells, both in vitro and in vivo. These findings are important milestones for the development of siRNA as a gene therapy for treatment of viral infections, cancer, autoimmune syndromes and numerous genetic disorders. This review describes the development of retroviral siRNA vectors, highlights proof-of-concept experiments demonstrating their therapeutic efficacy and explores therapeutic targets particularly suitable for retroviral-mediated gene silencing.Expert Opinion on Biological Therapy 04/2004; 4(3):319-27. · 3.35 Impact Factor - [show abstract] [hide abstract]
ABSTRACT: The human immunodeficiency virus type 1 integrase protein has karyophilic properties; that is, it localizes to the cell nucleus according to a range of assays. As an essential component of the preintegration complex, it has been suggested that the karyophilic properties of integrase might facilitate transport of the preintegration complex through the nuclear pore complexes of nondividing cells. However, no experiments have satisfactorily identified a nuclear localization signal within integrase. In this work, we investigated the karyophilic properties of integrase in intact cells with hopes of identifying a genuine transferable nuclear localization signal. Our results confirm that integrase tightly binds chromosomal DNA in vivo. However, our analysis determined that large integrase fusion proteins are unable to access the nucleus, indicating that integrase might lack a transferable nuclear localization signal. In addition, we present several lines of evidence to indicate that DNA binding might facilitate integrase nuclear accumulation. Furthermore, our data indicate integrase is degraded in the cytoplasm by a proteasome-dependent process, an event that probably contributes to the apparent nuclear accumulation of integrase. These results provide new insight into human immunodeficiency virus type 1 integrase intracellular dynamics.Journal of Cell Science 12/2003; 116(Pt 21):4401-8. · 5.88 Impact Factor - [show abstract] [hide abstract]
ABSTRACT: Retroviral replication requires the integration of reverse-transcribed viral cDNA into a cell chromosome. A key barrier to forming the integrated provirus is the nuclear envelope, and numerous regions in human immunodeficiency virus type 1 (HIV-1) have been shown to aid the nuclear localization of viral preintegration complexes (PICs) in infected cells. One region in integrase (IN), composed of Val-165 and Arg-166, was reportedly essential for HIV-1 replication and nuclear localization in all cell types. In this study we confirmed that HIV-1(V165A) and HIV-1(R166A) were replication defective and that less mutant viral cDNA localized to infected cell nuclei. However, we present three lines of evidence that argue against a specific role for Val-165 and Arg-166 in PIC nuclear import. First, results of transient transfections revealed that V165A FLAG-tagged IN and green fluorescent protein-IN fusions carrying either V165A or R166A predominantly localized to cell nuclei. Second, two different strains of previously described class II IN mutant viruses displayed similar nuclear entry profiles to those observed for HIV-1(V165A) and HIV-1(R166A), suggesting that defective nuclear import may be a common phenotype of replication-defective IN mutant viruses. Third, V165A and R166A mutants were defective for in vitro integration activity, when assayed both as PICs isolated from infected T-cells and as recombinant IN proteins purified from Escherichia coli. Based on these results, we conclude that HIV-1(V165A) and HIV-1(R166A) are pleiotropic mutants primarily defective for IN catalysis and that Val-165 and Arg-166 do not play a specific role in the nuclear localization of HIV-1 PICs in infected cells.Journal of Virology 12/2002; 76(21):10598-607. · 5.08 Impact Factor -
Article: Retrovirus-delivered siRNA.
[show abstract] [hide abstract]
ABSTRACT: The ability of transfected synthetic small interfering (si) RNAs to suppress the expression of specific transcripts has proved a useful technique to probe gene function in mammalian cells. However, high production costs limit this technology's utility for many laboratories and experimental situations. Recently, several DNA-based plasmid vectors have been developed that direct transcription of small hairpin RNAs, which are processed into functional siRNAs by cellular enzymes. Although these vectors provide certain advantages over chemically synthesized siRNAs, numerous disadvantages remain including merely transient siRNA expression and low and variable transfection efficiency. To overcome several limitations of plasmid-based siRNA, a retroviral siRNA delivery system was developed based on commerically available vectors. As a pilot study, a vector was designed to target the human Nuclear Dbf2-Related (NDR) kinase. Cells infected with the anti-NDR siRNA virus dramatically downregulate NDR expression, whereas control viruses have no effect on total NDR levels. To confirm and extend these findings, an additional virus was constructed to target a second gene, transcriptional coactivator p75. The experiments presented here demonstrate that retroviruses are efficient vectors for delivery of siRNA into mammalian cells. Retrovirus-delivered siRNA provides significant advancement over previously available methods by providing efficient, uniform delivery and immediate selection of stable "knock-down" cells. This development should provide a method to rapidly assess gene function in established cell lines, primary cells, or animals.BMC Biotechnology 09/2002; 2:15. · 2.17 Impact Factor
Top Journals
Dana-Farber Cancer Institute
- Department of Cancer Immunology and AIDS
Boston, MA, United States -
University of Massachusetts Boston
Boston, Massachusetts, United States
Harvard Medical School
- Department of Biological Chemistry and Molecular Pharmacology
Boston, MA, United States


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Ci Racing Hack and Cheat Information
Added on: Nov 14, 2012
Play Count: 3999
Category: Racing
Hack Information:
Key Hacks: [1] - Win Level [2] - Add Upgrade Points
Drive your anti gravity vehicle around the various stages but actually rotate the the landscape as you go for a unique new gaming experience.
Rating : 8 out of 10 based on 15 user ratings.
Game not working? If you see a stolen game sign you must be using a proxy! Report it.
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