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Here we go again.
With official Washington trapped in partisan gridlock, doctors who treat Medicare patients are once again facing the prospect of a big cut in pay that almost no one supports.
And this time Medicare officials say they won't be hold onto the bills for longer than the usual 10-day processing time to wait for Congress to act. A 27 percent cut is set to take effect Jan. 1, unless Congress stops it.
The last time Congress was deadlocked over what's come to be known as the "Medicare doc fix" was in the spring and summer of 2010, when the threatened cut was a mere 21 percent, and Congress ended up delaying it a month at a time for several months.
When Congress actually missed one of the deadlines, Medicare officials stepped in, and sat on Medicare claims for several days, to give lawmakers a little more to pass the extension just about everyone knew was coming.
But that's not an option this time, said Jonathan Blum, deputy administrator of the Centers for Medicare and Medicaid Services and director of the Center for Medicare. "The last time when we did the extended claims hold we nearly crashed the system, and so we feel very strongly that can never happen again," he told Shots in an interview Tuesday.
The problem is that even though Medicare claims are paid by nearly a dozen separate insurance company contractors around the country, "the claims run through common systems, so we can do all the fraud and abuse controls and all the payment checks to ensure the payments are accurate and appropriate."
And because those systems are "only built to handle so much volume," the backlog that developed from holding up several weeks of claims last time almost turned catastrophic, Blum said.
There's another reason Blum said Medicare has decided not to sit on claims for such a long time again. After the last go-round, he said, officials got "very strong feedback" from physicians, particularly those in small practices that "the extended claims hold created severe cash-flow problems." They made it clear, he said, "they would rather have some cash flow to ensure that they can keep afloat during a very challenging time."
So, Medicare contractors have been told to hold claims exactly 10 business days (which current law gives Medicare authority to do), bringing the drop-dead date to Jan. 18, coincidentally the day after the Senate is scheduled to return to work.
If the standoff isn't resolved by then, Medicare will begin processing payments at the lower rate. Should Congress eventually cancel the cut (which appears likely), then it would have to gave Medicare specific authority to reprocess those claims at the higher rate.
Said Blum: "That's added work and cost to the program, obviously."
Copyright 2021 NPR. To see more, visit https://www.npr.org.


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subset: commoncrawl


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Our company has to date delivered 40 proposals [approximately $350,000 in bids] and have secured all of them. I give credit to Proposal Pack for helping me deliver these in a timely manner."
While the Proposal Kit Professional is our main product, a lower cost option is to get a single Proposal Pack design theme such as this one and optionally upgrade to the Professional bundle later if you need the extra contract content or more advanced software features.
Proposal Pack Resources #2 is a specialty graphic logo design which accents our entire collection of over 2200 business proposal templates. You get a custom design theme consistently integrated through every page of your proposal. We have done most of the work for you already - pre-written proposal letters, contracts and documents complete with graphic designs and title page backgrounds and sample proposals which show you how to create your own.
Use the templates as-is, edit the graphics for a custom look and use the optional Proposal Pack Wizard automation software to master your business proposal writing.
Design elements in the Proposal Pack templates such as colored borders can be easily changed in your word processor. You can change the size, shape and color of the borders, fonts, tables and other elements.
Other design elements such as logos, body watermark graphics, custom bullet point graphics and Title Page graphic designs can also be customized. Proposal Packs include the original artwork graphics to make it easier for you to customize our Proposal Pack graphic themes. This type of customizing requires graphic design work using your own graphics editing software. We also provide services to customize Proposal Pack graphic themes.
Logos and images included in the Proposal Packs are general purpose and are designed for you to replace with your own logo and images. Stock photography and logos may not be used outside of the Proposal Pack templates for any other purpose.
Proposal Packs are downloadable after ordering. Platforms supported include Microsoft Windows, Mac, Linux, Microsoft Word, Microsoft Office, OpenOffice, StarOffice, Word Perfect, Google Docs, iWork, Pages and Appleworks. Proposal Packs are for use with Microsoft Word for Windows or any other word processor than can open Microsoft Word documents.
You can output your completed documents in any format your word processor supports. For example, when using Word you can output in Word, PDF, HTML, XML and text formats. When using Pages on the Mac you can output in Word, PDF and Pages format.
Also includes many sample proposals, general project and service contracts and non-disclosure forms. Supports creating general business proposals, grant funding proposals, business plan funding proposals, book publishing proposals, letter proposals, government contracts, research proposals, resumes and other complex business documents. The library of included sample proposals covers many different types of proposals in many industries and are included as general guides and help information.
Designed for use by any type of business, small and large. Proposal Packs are the framework used to show your prospective clients who you are, what you can do, what you propose to do for them, and most importantly, how they will benefit from the product or service you provide. Each Proposal Pack includes over 5500 pages of templates, samples and instructions.
The Proposal Pack templates and documents work on all platforms including Mac. You can open the documents in Word for Mac, OpenOffice for Mac, etc. There is an interface that runs on a Mac browser to help navigate through all this content.
We already include several assembled documents for product and service sales proposals, business plans, grants, quotes, etc. ready to open and edit as-is on a Mac.
The Novice Wizard that comes with the individual Proposal Packs is an automation tool that is built using Word for Windows. Microsoft does not support its macro automation features on their Word for Mac. So, this interface is only used on Windows platforms. This interface is also mainly used to custom build short templates more efficiently. If you have access to a Windows computer running Word for Windows you can install it there, create custom templates then edit those templates on your Mac.
To assist Mac users who do not have any access to a Windows computer we will generate one custom template for you using any of our hundreds of document layouts. You can request this when you are ready after ordering and downloading the Proposal Pack.
your packs today and finished a business proposal in one day that would otherwise have taken me a week to do and the result was far more professional looking..."
summarize in the best of fashion all the finest practices for businesses in their dealings with customers, mostly because they remain focused on the client's solution, giving back to the customer the control over his project."


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BMO Global Asset Management and global technology firm FIS announced Wednesday that they have formed a strategic alliance with California-based Farmers and Merchants Trust Co. to provide Farmers with what they say is an "industry-leading" trust and custody platform.
Under the partnership, BMO and FIS will cooperatively provide Farmers and Merchants Trust with a trust and custody platform developed by FIS' technology specialists and serviced by BMO Global Asset Management's dedicated team of trust operations and custody professionals.
"The combination of FIS and BMO Global Asset Management links the industry-leading custody platform with a premiere trust outsourcing service provider," said Frank Tabar, VP and Strategic Risk and Operating Officer for Farmers and Merchants Trust. "Together, their proven track record in product development and client service gives us the ability to focus on serving our current and future customers."
Wayne Klomstad, director of Trust Outsourcing-Trust and Custody Services for BMO Global Asset Management, added in the same statement that for nearly a century, "Farmers and Merchants has provided clients throughout California with service excellence. Their commitment to their clients closely mirrors that of the relationship between BMO and FIS. Together, we will strive to provide best-in-class trust and custody solutions to multiple generations of clients throughout California."
Under the agreement, FIS will be providing a fully integrated trust and wealth management technology platform including fiduciary accounting and reporting, front-office administrative and management portals, automated compliance tools, real-time portfolio management, daily performance measurement, and real-time client and third-party web-based account access and transaction initiation.


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Crystals for Control
Crystals for Control
Control is a tricky trait to master and I would nearly say no one has truly mastered it. The problem with control is, just as with many things, too much of one thing can be bad. It is important to have control in your life, especially if someone has taken it before from you in the past. When we gain too much control, we become overbearing and controlling. So where can we strike a balance? We believe crystals can help with this. Whether you have too much control and you need to try and step back or if you need to regain control that was lost or maybe never even had, the right crystal can help realign you where you need to be.
Midsummer is a time in the year when we rejoice in the energy of the sun and the growth it creates, which nourishes the body and the soul. The rays of a golden crystal fulfill us and give us the enthusiasm and excitement that make life a wonderful adventure. Gold is traditionally the color of kings, riches, and the sun. It is all this and more. Gold is as close to a magical color as exists. It seems to touch a deep part of our minds, conjuring up images of mystical places, treasure, and adventure.
Use Gold Crystals
Golden Crystals Bring You Success, Enthusiasm, Happiness, and Power
Gold is the color of adventure, success, and power. It is the ray of rarity, bespeaking richness and natural value. It enriches the spirit, warms us like yellow, and cheers us like orange. Gold is the high color, the crown of life. It is all life, sweet, warm; growing and reaching its peak. However, it is in darker shades of gold that control can be accessed and utilized. In darker shades, the golden crystals have a deeper and more pronounced sense of devotion and commitment, providing us with a mature enthusiasm and the ability to share a lifelong commitment of care and love. They provide us with the power to lead projects, organizations, and efforts to success. Most important, darker gold crystals such as Pietersite and Lemurian Seed Crystals are the ultimate power crystals. They exude authority, control, leadership, success, and influence.
Pietersite is called the "tempest stone" because it has been folded and stressed within the earth and yet emerges as truly beautiful. It is a calming agent; a powerful meditation stone that often brings almost instant relief from stress and elevated levels of negative energy. Pietersite specifically brings you power over your inner self allowing for you to control your stress and anxiety. Just as lightning clears the air and makes all things new, Pietersite discharges negative energies and emotional turmoil, and cleanses the aura, restoring it to calm.
Lemurian Seed Crystals are said to have originated in a country known as Mu or Lemuria. This civilization, according to the legends, was a peaceful and highly developed spiritual civilization that existed over 12 million years ago in the area of the South Pacific. The area is now beneath the sea. The Lemurians are believed to have foreseen a cataclysmic event, and prepared the Seed Crystals to preserve their knowledge and traditions. Lemurian Seed crystals are often used meditation crystals. They are reported to contain extensive amounts of knowledge and understanding of the natural universe. They are considered to be the repositories of the knowledge of the ancient Lemurians. Meditation with these crystals allows for the ancient healing knowledge to pass into your consciousness.
Pietersite is a major meditation stone used extensively to calm, de stress, and soothe the user. Because of its tumultuous origins it brings the power to overcome stress and hardship.
Lemurian Seed
Lemurian Seed crystals have been used extensively in healing efforts associated with the heart chakra. It can be specifically used in spiritual healing when the entire organism is out of balance with itself.
What Authors Say About Crystals
Judy Hall recommends Agrellite which is effective at bringing hidden matters to the surface.
Useful for psychological healing and for overcoming writer's block, Agrellite highlights your attempts to control others, supporting independence and self-respect in both parties.
Control can be a very important part of our lives. We may live with the desire to be controlling or maybe you have been controlled in the past and it has dwindled down our own ability to be more controlling. Whichever the case may be for you, we feel dark gold crystals such as, Pietersite and Lemurian Seed Crystals can aid you in your quest for bringing your control to a better balance. You can also try crystals other authors have recommended such as Agrellite.


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VW Amarok 3.0L 2.0L 2.5 inch Monotube IFP Front ONLY Coilover Kit
Please take the time to read through our returns policy. Upon purchase, you agree to this policy and understand that our policy does not go against your rights detailed under the Australian Consumer Law.
By purchasing a product from 4X4OC, your purchase of goods come with automatic guarantees that they will work and do what you asked for. If you buy something that isn't right, you have consumer rights which you can find here.
In line with your consumer guarantee rights, repairs, replacements or refunds do not apply if you received what you asked for but simply:
- changed your mind;
- found it cheaper somewhere else;
- decided you did not like the purchase; or
- had no use for it.
Should you change your mind within a 14 day timeframe, we will provide you with a refund or store credit.
Please note that, shipping costs are not refundable for change of mind returns. You are also responsible for covering the costs to send the product/s back to us.
Minor problems may be repairable, where we may offer a free repair instead of a replacement or refund. For any major problems, you have the right to request either a refund or new replacement.
Should we not be able to provide a free repair within 28 days, we will provide you with a full refund or replacement.
We understand that some products may fail over time due to their purpose/intended use or treatment. 4X4OC will take the following into account prior to offering a replacement or refund:
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Should we discover signs of misuse and significant neglect, we reserve the right to decline a refund, exchange, credit or repair.
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Once your return is received and inspected, we will send you an email to notify you that we have received your returned item. We will also notify you of the approval or rejection of your refund.
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Next contact your bank. There is often some processing time before a refund is posted.
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To return your product, you should mail your product to:
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You will be responsible for paying for your own shipping costs for returning your item/s. Shipping costs are non-refundable. If you receive a refund, the cost of return shipping will be deducted from your refund.
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If you are shipping an item over $75, you should consider using a trackable shipping service or purchasing shipping insurance. We don't guarantee that we will receive your returned item. We are not responsible for lost, stolen or undelivered returned parcels.
Please note, for all items where the "Add to cart" button is replaced with "Pre-Order", this is a pre-order item. A warning box will appear with terms and conditions of the pre-order which states the estimated delivery time.
Please be patient once a pre-order has been made. As the stock is not on hand, we have zero control of the delivery times until we receive the stock.
Pre-ordering is a great way to lock in the hot selling products which generally sell out/are totally sold out once the stock arrives.
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If you haven't received your order by the ETA, please feel free to email firstname.lastname@example.com so that we can do our best to follow up on your order with the courier company.
We ship with various shipping providers to best suit the product ordered. We mostly use:
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Some larger orders may be dispatched from the supplier directly where alternative shipping companies are used. This saves on double handling, reduces shipping time and overall reduces our carbon footprint. The courier being used will be stated on the checkout page and when you receive your tracking details.
Please note: timeframe estimates are from when the parcel is sent and only account for business days.
All orders are processed and sent as soon as we can. The time frame may include delays out of our control. International orders may even arrive faster than the 10 days minimum depending on the current climate and demand.
Should you have any shipping related enquiries, please do not hesitate to get in contact with us. Our aim is to provide a smooth and professional service at all times.


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NT0771 : Badger Meadows
taken 11 years ago, near to Broxburn, West Lothian, Scotland
Following the completion and opening of the Forth and Clyde Canal in 1790, it was not long before the idea arose of a canal to link Edinburgh to the west, and in particular to the coal mining areas in the west of Scotland, to enable coal to be supplied more cheaply to the capital. Other commodities suited for canal transport included building stone and lime.
It took another two decades, however, before a survey was undertaken of a route that would link the proposed canal to the Forth and Clyde Canal at Falkirk. The necessary Act of Parliament authorising construction was passed in 1817, and work started at the Edinburgh end, at Port Hopetoun, the following year.
The canal was designed by Hugh Baird Link and Thomas Telford Link and Link on behalf of the Edinburgh and Glasgow Union Canal Company, whose 384 shareholders included the Lord Provost of Edinburgh and a number of titled persons.
The canal was originally 32 miles in length and 5 feet deep, and it is a contour canal, that is, it follows the 241 foot (73 metre) contour, except for three river valleys where major aqueducts had to be built to carry the canal across at this height. These are the Slateford Aqueduct over the Water of Leith, the Almond Aqueduct 75 feet (23 metres) above the River Almond at Lin's Mill, and the longest of all at 810 feet (250 metres), the Avon Aqueduct, 86 feet (36 metres) above the River Avon near Linlithgow.
Most of the 60-odd bridges are fixed, so neither locks nor opening bridges delayed navigation most of the way, though the aqueducts were only wide enough for boats and barges to travel in one direction at a time; oncoming vessels had to wait until the way was clear.
A tunnel 690 yards (636 metres) in length was required near Falkirk because the owner of Callendar House, William Forbes, refused to allow the canal to pass through his land, and this undertaking contributed to the final cost being almost double the original estimate of £240,500. The final bill of over £460,000 equates to anything between £2000 million and £380,000 million at 2014 prices, depending on how it is calculated.
The water level is maintained from a reservoir in the Pentland Hills, a three-mile channel from the River Almond, and other feeders.
At Falkirk the Union Canal was 110 feet (33 metres) above the level of the Forth and Clyde Canal, and a flight of eleven locks was built to link the two canals.
Construction took just four years, and the canal opened for traffic in 1822. Tolls were charged for the carriage of goods, and fares for passengers. There was also income from wharfage. Maximum charges were laid down in the enabling Act of Parliament. The Company was required to pay duties on goods carried to the Burgh of Linlithgow and the City of Edinburgh because the canal took trade away from the turnpike roads maintained by the respective councils.
Early trade was brisk, but unfortunately its heyday lasted only about 20 years, until the Edinburgh and Glasgow Railway superseded it in 1842, and in 1849 the canal was acquired by the railway company. Its use declined steadily over the years, and in the 1930s the flight of eleven locks at Falkirk was filled in and built on. Other stretches of the canal in Edinburgh were also built on, including the Edinburgh terminus. The City Bypass cut the route west of Edinburgh, and the M8 motorway blocked it near Broxburn. The canal was officially closed in 1965, though various stretches were used recreationally, for example for rowing and angling.
Towards the end of the 20th century there was a revival of interest in canals, mainly for recreational use, and the restoration of the Forth and Clyde Canal and the Union Canal was mooted. The project was adopted as one of the major undertakings to mark the millennium, and obtained funding from the European Union and the Millennium Commission among others. A new terminus with associated new retail, residential and commercial buildings was created in Edinburgh and a new stretch of canal dug to replace the section built on at Wester Hailes. The Scott Russell Aqueduct (named after John Scott Russell, who first observed a soliton wave on the canal in 1834) now carries the canal over the City Bypass, and a diversion was built to pass beneath the M8 motorway. The final piece of the restoration was the unique Falkirk Wheel, at the end of an extension to the original canal at Falkirk, which lifts and lowers boats between the Union Canal and the Forth and Clyde Canal.
The Millennium Link restoration cost some £84 million and the restored canal was opened in 2001. The Falkirk Wheel was opened by Her Majesty Queen Elizabeth the following year.


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Estimated read time: 4-5 minutes
This archived news story is available only for your personal, non-commercial use. Information in the story may be outdated or superseded by additional information. Reading or replaying the story in its archived form does not constitute a republication of the story.
LITTLETON, Colo. (AP) - A suburban Denver high school student who was shot in the head by a classmate died Saturday afternoon, hospital officials and her family said.
Claire Davis, 17, was in critical condition after being shot at point-blank range at Arapahoe High School on Dec. 13.
"It is with heavy hearts that we share that at 4:29 p.m. this afternoon, Claire Davis passed away, with her family at her side," a statement from Littleton Adventist Hospital said.
"Despite the best efforts of our physicians and nursing staff, and Claire's fighting spirit, her injuries were too severe and the most advanced medical treatments could not prevent this tragic loss of life. Claire's death is immensely heartbreaking for our entire community, our staff and our families."
The Davis family said in a statement that they are grateful for the 17 years they had with their daughter.
"The grace, laughter and light she brought to this world will not be extinguished by her death; to the contrary, it will only get stronger," the statement said.
The family said they appreciated the outpouring of support from the community and thanked the efforts of law enforcement, school officials and medical staff.
The date for a public memorial to honor the teen would be announced later, the hospital's statement said.
Friends and well-wishers had posted prayers online and raised money to help pay for Claire Davis' medical care in days following the shooting. On Friday, about 100 people gathered at a church near the school to pray for the teen.
The Denver Foundation said a fund had been created honoring her that would support the high school and community in programs for mental health, anti-bullying and other needs.
Karl Pierson, 18, shot Davis, who just happened to be sitting nearby with a friend as Pierson, armed with a shotgun, ammunition strapped to his body, Molotov cocktails and a machete, entered the school and headed toward the library. Davis appeared to be a random target, Arapahoe County Sheriff Grayson Robinson has said.
Pierson likely intended to track down a librarian who had disciplined him, but Robinson said Pierson's arsenal suggested Pierson intended to hurt many others at the school just 8 miles from Columbine High School.
Pierson set off one of the incendiary devices and fired five shots before killing himself just one minute and 20 seconds after entering the building. He knew a sheriff's deputy assigned to the school was closing in, Robinson said at a news conference.
Senior Chris Davis, who is not related to Claire Davis, said she loved horses, had a lot of friends and always seemed happy. Chris Davis, whose locker is next to Claire's, helped organize a fundraising effort for her family.
Students held vigils for Davis after the shooting. Typical was a story told by classmate Maggie Hurlbut.
"One time I remember I was upset in the hallway, and she came up to me and she just _ it was like, Hey Maggie, I know we don't know each other well but are you doing OK?' And I told her yeah, and she was like,
Anything you need, I'm here for you,'" Hurlbut said. "Again, that's who she is, and she just wants to take care of others, and that was really just a good representation of her character and who she was."
Gov. John Hickenlooper visited Davis and her family at the hospital and had asked for prayers.
Sheriff Robinson called Davis "a young woman of principle" and "an innocent young lady."
Pierson's original target was believed to be a librarian who coached the school's speech and debate team. Pierson was a skilled speaker and debater on the team. The librarian, whose name was not released, had disciplined the teen in September for reasons that haven't been disclosed. Robinson said Pierson had made some sort of threat against the librarian in September.
"We are looking into that, to the degree that it was understood, and then what interactions or interventions took place," the sheriff said.
The librarian was able to escape the school unharmed, Robinson said.
Pierson legally purchased his shotgun at a local store a week before the shooting and bought the ammunition the day of the shooting. Anyone 18 and older is allowed to buy a shotgun in Colorado; only those over 21 can legally buy a handgun.
Pierson, whose parents were divorced, lived at least part of the time with his mother in a higher-end neighborhood in suburban Highlands Ranch.
The Arapahoe shooting came a day before the one-year anniversary of the Newtown, Conn., attack in which a gunman killed 20 children and six adults at Sandy Hook Elementary School.
(Copyright 2013 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.)


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About us
Hi! We are Tin Rose Boutique, a small shop that has been thriving off our customer relationship for almost 5 years. We strive to offer something for woman of all shapes and sizes. We are located in the heart of Texas, South Fort Worth. Right next to C.B. Finchers Western Wear. We have a small team of 6 that help this puzzle come together. Natalie Rice is the owner who has been in retail all her life since she was little working at her fathers store, Finchers. So it only came second nature for her to build something of her own. You wont just see her at the shop, but doing hair, working horses with her husband, barrel racing in her free time, or spending time with her little ones.


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Sweet Potato Biscuits
Am I the only one eschewing the perennial
It's January, people. It's still winter. Some folks are shoveling snow. Enough already with the bunny food. Dump the word cleanse from your vocabulary.
Have a biscuit.
Have a biscuit.
You can whip up all the low calorie high fiber cleansing smoothies you want if they help you feel virtuous and powerful and fresh as a daisy. I've got nothing against smoothies. I'm a fan of creamy breakfasts, actually. And I love antioxidants as much as the next gluten-free soul (bring on the blueberries, Buster). But I chafe at the word diet. And should. And so I resist the collective push to declare my commitment with a list of New Year's Resolutions for a Better Me.
If I did make a list, though?
It might look something like this...
It might look something like this...
Top Ten Gluten-Free Goals for the New Year
1. Make more biscuits. I'm serious. Warm, fall apart, tender, melt-in-your-mouth biscuits. Life is too short to live without biscuits.
2. Drink more Champagne. It's silly to wait for a special occasion. Every day deserves Champagne.
3. Walk more. And I don't mean power walk. I don't mean buying a pedometer. I mean walk as in stroll. Amble. Look at the sky. People watch. Notice the way the late afternoon sun resembles the color of honey.
4. Play around with some donut recipes. Start with those old fashioned blueberry cake donuts that have a hint of cinnamon and nutmeg.
5. Listen to more music. Discover new artists.
6. Dream up a fabulous gluten-free bialy. Just chant: Yes, I can.
7. Go on more picnics. See goal #2.
8. Take more photos. For fun.
9. Stop feeling guilty for (fill in the blank). Just do it. I said so.
10. Breathe, Baby, breathe. Everyone's doing the best they can to get through the day. And those of us with celiac? We're doing double.
Karina's Sweet Potato Biscuits Recipe
Recipe posted January 2010.
Make this dough lickity split with a sturdy stand mixer or do it the old fashioned way- with an elbow grease powered wooden spoon.
In a mixing bowl, combine the dry ingredients and whisk them together:
1/2 cup sorghum flour
1/2 cup potato starch (not potato flour)
1/4 cup millet flour
1 tablespoon baking powder
1/2 teaspoon baking soda
1 teaspoon sea salt
1/2 teaspoon xanthan gum
Pinch nutmeg, to taste (don't put in too much!)
Add in:
3 tablespoons Spectrum Organic Shortening
1 cup canned sweet potato or finely mashed cooked sweet potato
2 tablespoons pure maple syrup
1 1/2 teaspoons Ener-G Egg Replacer whisked with 2 tablespoons warm water, till frothy
Add in:
3 tablespoons Spectrum Organic Shortening
1 cup canned sweet potato or finely mashed cooked sweet potato
2 tablespoons pure maple syrup
1 1/2 teaspoons Ener-G Egg Replacer whisked with 2 tablespoons warm water, till frothy
Beat to combine. The dough will be smooth and sticky.
Preheat the oven to 375ºF. Prepare a muffin pan (if the pan is non-stick, no need to do anything). You'll be using 9 of the cups.
Spoon the biscuit dough into nine muffin cups. Shape the tops with wet fingers, if you like (I left mine like drop biscuits, a little rough and bumpy).
Bake in the center of a pre-heated oven for 15 to 20 minutes or so- until firm and slightly golden. A wooden pick inserted into the center will emerge clean and the bottoms will be golden and firm. If in doubt, bake for another 3 minutes.
Pop the biscuits out of the muffin pan.
Indulge while they are still warm. Slather with maple butter, apricot jam, whipped honey...
Makes 9 biscuits.
I tried freezing a few and reheating them in the microwave. They were not nearly as good. This is one of those recipes you make and enjoy. Live in the now.
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Recipe Source: glutenfreegoddess.blogspot.com
All images & content are copyright protected, all rights reserved. Please do not use our images or content without prior permission. Thank you.
More gluten-free sweet potato biscuit recipes from food bloggers:
Sweet Potato Biscuits - Gluten-Free at Gluten A Go-Go
Classic Bisquick based biscuits you can tweak to make gluten-free (use a gluten-free baking and pancake mix):


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"I'm a whole lot better and still just as dangerous. I'm fighting because I want to be the best in the world. I want to make a run at that."
Over a 13-year career, Yves Edwards has emotionally surfed the highs and lows of the fight game, and the Bahamas native envisions a glorious resurgence as he hunts his second straight UFC victory at this month's Fight for the Troops event in Fort Hood, Tex.
The lightweight pioneer, who faces unbeaten guillotine master Cody McKenzie (12-0) on the show's undercard, established himself as a force to be reckoned with at the early turn of the new millennium by conquering formidable lightweights such Hermes Franca (twice) and Josh Thomson. Armed with an impressive 30-8-1 record and knockout power, the superbly athletic Edwards worked his name into the mix with BJ Penn, Takanori Gomi and Genki Sudo in discussions about the world's top 155-pounders. That was back in mid-2005. Since that time, however, Edwards has only managed a disappointing 9-8 record. But now, even at age 34, the dreamer inside of the affable islander remains.
"A lot of it had to do with confidence and also mistakes preparing for fights," said Edwards, winner of five of his past six bouts. "I lost a lot of confidence at one point in my career and I honestly didn't know where I was going. I've made some changes and I don't think I'm that guy any more. Will I lose again? It's possible, but I don't see it happening and I'm going to do everything in my power to keep that from happening. I'm a whole lot better and still just as dangerous. I think that guy is going to show up. I'm fighting because I want to be the best in the world. I want to make a run at that."
It matters not to Edwards whom he must go through to get back to the top. If it's a friend or sparring partner, so be it. Edwards had been game to battle his buddy, Melvin Guillard, at the UFC Fight for the Troops event. But when an injury bumped Kenny Florian from the show's main event, Guillard was inserted as a replacement opposite Evan Dunham, forcing Edwards to switch gears and prepare for a grappler instead of a striker. Edwards, a married father of two who lives in Austin, took the bout change in stride.
"Melvin is ranked higher in any of the rankings than Cody," Edwards noted, "so, yes, it's a step down as far as the ranking of the opponent. But competition is competition and I'm sure Cody is coming to fight, so I can't be thinking about Melvin anymore."
McKenzie, the former fisherman whose carefree vibe is reminiscent of the fictitious surfer dude character "Jeff Spicoli" from the 1980s movie classic "Fast Times at Ridgemont High," would seem to be at a decided disadvantage if he is forced to stand too long with Edwards, owner of 14 knockouts. Of course, stranger things have happened in the MMA game. Along with his standup acumen, Edwards possesses remarkable well-roundedness, too, as evidenced by his 16 submission victories.
Asked if there has been a particular style of fighter that has given him the most problems throughout his career, Edwards paused momentarily.
"Wrestlers," he said. "Wrestlers."
McKenzie, a veteran of The Ultimate Fighter season 12 cast, hasn't shown much in the wrestling department. The free-wheeling Alaskan is a wild man who loves to induce chaos inside the cage. Eventually, amid that frenzy, opponents shoot on McKenzie and then pay dearly for it when trapped in the clutches of his trademark "McKenzietine" choke.
"He's a submission guy, a tough guy," Edwards acknowledged. "He's going to try to create things on the ground and that makes for an entertaining fight. It's the guys that try to stall you out that give me problems. There may be openings for both of us and I'll try to take advantage of them before he can."
Edwards added that he rarely shoots on foes and tries "to do all my tapping in the gym." In fact, his submission defense has been top-notch. Edwards hasn't been subbed in a live fight in almost five years. According to Edwards, he was born to be a fighter. As proof, he points to the day of his birth – Sept. 30, 1976. He was born one year and one day removed from the "Thrilla in Manila," the rubber match between Muhammad Ali and Joe Frazier that is often hailed as the greatest boxing match of all-time.
"The doctor told my mom the day I was born that I was going to be a fighter," Edwards said. "The doctor that delivered me was a big boxing fan. It never occurred to me but I remember my dad bought me some boxing gloves when I was a kid, hung a pillow case up with some clothes in it and let me beat it up. That was a lot of fun. But I didn't really expect to be a fighter. I wanted to play basketball. I played basketball all through high school in the Bahamas. But as I got older I started to practice martial arts and I found myself in the martial arts."
Edwards was born into an upper middle-class family. His mother ran a flower shop, his father had a very unique profession.
"My dad was an entertainer," he said. "My dad danced limbo back in the Bahamas. He was one of the top entertainers in the country. He's performed for Nelson Mandela and diplomats from around the world who came to visit. He did comedy while he did his limbo dancing. He started with the bar really, really high and as he bought the bar lower and lower he would progress with his jokes. It got to the point where he would have the bar six inches from the floor and on fire, spinning two trays that were also on fire, and he would go under that bar. … Nobody did it like my dad did it.
"From all the stories I got from my aunts and uncles, he was always an athlete. They tend to point toward him whenever they see me do something pretty cool. They'll say, 'You're just like your dad.' Which is very flattering because who doesn't want to be like their dad?"
Sadly, Edwards' memory of his father is confined to his childhood; his father passed when he was just 12 years old.
"He was supposed to pick me up from school," Edwards recalled. "I heard his name on the radio. I was taking an exam and my mom came up to the school. I saw her walking up toward my classroom crying, so I knew …"
Edwards and his mother moved to the United States when he was 15.
"There were some opportunities in the Bahamas but not a lot," he said. "She made the move for my benefit. I didn't want to move to America at the time."
Today, the adopted Texan considers himself a happy and content family man. He trains in Austin with Tim Kennedy, Kamal Shalorus and Phil Cardella, and also supplants that training with extended training sessions in Florida with American Top Team.
"Life is awesome. My wife is a hard worker, she's working on her Ph.D," Edwards said. "I have a son who I love very much. My daughter is 16 now, which is crazy because my daughter is beautiful and that's kind of hard to deal with. It was awesome when she was a kid but now, you know, boys are interested and stuff like that."
All that is missing, it seems, is for Edwards to re-establish his glory in the Octagon. It's been a long road back to the UFC. He's hoping this time around will cement his legacy. He's hoping to make liars of his doubters.
"It means a lot. The UFC is a completely different monster now," he said. "It's funny because the 155 pound weight class in 2004 basically consisted of like six guys. Now the UFC 155 pound weight class is the biggest weight class in the company, it has the most guys on the roster. So I'm really excited about that. The sport is so much bigger, and being in the biggest organization in the sport gives me a chance to be the number one guy in the world. I love the competition and I want to look at back and ultimately say, 'I was the best in the world at that.' And if I can't say that then I want to say, 'Man, I did everything I could to be the best in the world at that.'"
UFC Performance Institute
Dana White On The Opening Of UFC PI Mexico


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2008: A Year in Review, Leaky and the Fandom
Dec 30, 2008
Time for the second year in review report, this focusing on events and moments for us here at Leaky and the Harry Potter fandom.
A new year brought a new poll on the Wizarding World of Harry Potter website, and reports claimed a December 2009 opening is possible.
The Ace of Cakes met PotterCast as the Food Network star Duff joined John and Sue to talk about the very special cake he made for the Harry Potter and the Order of the Phoenix premiere last summer in Los Angeles. Audio and video of this are online. January 22 saw an announcement about the Wizard Rock EP of the Month for charity and the Harry Potter Alliance.
The month starts off great with news that Scribbulus, our premiere essay collection,
was updated and part of our continuing site redesign. Also we present THE LEAKY LOGIN: the one, all-purpose login you need around Leaky and our forums ' one login gives you access to post on our forum, Leaky Lounge, in our image, video and fan art galleries, and much more in the future.
More conference updates brought word of a Wrock contest for Terminus. On February 11 Construction begans on the Wizarding World of Harry Potter in Orlando, Florida.
February 12: Big News as we announce our first ever convention: LeakyCon 2009 in Boston, MA, Memorial Day Weekend, May 21-24,
a benefit for the charities Book Aid International and HP Alliance. (More info available at LeakyCon.com.) We also opened LeakyCon Facebook and LiveJournal groups.
The Lounge got lovely with a Valentine's Celebration, and the OotP Book Club also opened. Finally registration for the big Wizard Rock gathering, Wrockstock 08, opened.
The month opened with the 22nd issue of Scribbulus online. Canadian band Wyrd Sisters continue a legal claim against WB, despite an unsuccessful copyright claim against WB in 2005, now seeking destruction of DVDs, compact discs, video games and other paraphernalia" mentioning fictional wizarding band in things such as trading cards scene it games etc
Wizrocklopedia announced winners, two found on Jingle Spells our CD for charity issued in December of 2007. On the convention front, we learned US audio book narrator Jim Dale would be speaking at Portus, the Harry Potter gathering due in Dallas, and Terminus announced their keynote speakers, with Cheryl Klein, continuity editor of the Harry Potter books from Scholastic to speak along with author Tamora Pierce.
In a fun bit of news, Harry Potter Puppet Pals were big winners at the YouTube Awards. We made some additions to our Leaky Lounge, and made an announcement about the Floo Network. Media focus on Harry Potter continued, with features on CNN regarding Potter in Academia, and others feature college Quidditch. Finally rumors of early auditions to take place for characters at the Harry Potter theme park begin to surface.
Frankie "Frak" Franco permanently joins PotterCast, and on April 1st, we hire an unusual editor, Sybill Trelawney. Mayhem and odd reports surface regarding possible breakups of Harry and the Potters, and that Dan Radcliffe is to perform Equus fully clothed. Sadly, the end of the day sees Sybill sacked…again. Scribbulus also joins in the April Fools fun as well.
Jo's Book Club reads Pride and Prejudice. The TLC crafters started monthly crafting events, and the We are Wizards Documentary screening and wrock show was set for Boston.
This month, we openeded the LeakyCon forums. We also launch our Fallen Heroes tribute contest, and PotterCast pays tribute to our unsung (unvoiced?) heroes: the Transcript Elves! PotterCast was live down in Florida with some actors from the Harry Potter films, and the Wizard Rockumentary screened in the sunshine state as well. Our reading groups rediscovered Sorcerer's Stone, and Keynote speakers were announced for Convention Alley
The season of many conventions was upon us this month, and we updated on these, as TLC also launched its two part Riddle contest. June 13: The Quibbler, TLC's newest online magazine with the focus on fun and creativity from and for Harry Potter fans, arrives. We wants the precious, as our book club delved into J.R.R. Tolkein's classic The Hobbit, and our crafters make Hogwarts house friendship bracelets
June 19: HPA launche Wrock the Vote,
as the AP focuses on the efforts of the charity group of Harry Potter fans. Summer solstice marked five years since the release of Harry Potter and the Order of the Phoenix, and we remember and celebrate the life of Dobby the House Elf. June 28 sees the third year anniversary of The Chamber of Chat, the unique interactive chat for fans.
The holiday weekend features the Corner Booth movie night as we chat and watch Sorcerer's Stone online together. The chef from the Wizarding World of Harry Potter theme park gives a new interview, detailing and tempting us with some of the goodies we may find when the park opens. July 9 Portus 2009 is set to take place over the weekend in Dallas, Texas. July 12: LeakyCon announces a very special wizard rock concerts and dance parties to be held during the Terminus convention in Chicago next month, all to benefit the con and the charities. July 14 we saw construction photos updates and new signs appearing at Wizarding World in Orlando. The next day we learn Wizarding fans affect UN Security Council with HPA Initiative
In other news, PotterCast and Leaky will feature at Comic Con in San Diego, and Dr. Horrible learned his evil ways from Voldemort.
The 19th we open registration for Leaky Con and hotel reservations also open. The Balmoral hotel in Scotland also opened the J.K. Rowling suite where she completed Deathly Hallows, as on the 21st we marked one year since the release of the final Harry Potter novel.
July 23 we present Leaky Blogs written by staff and readers of the Leaky Cauldron, and a few days later we announced PotterCast's Summer tour 08, as we hit the road with The Remus Lupins, The Whomping Willows and the Mudbloods. First online: Boston stop! The month ends on a high note as Melissa announced her big book project: Harry, A History would be published this fall on November 4.
Universal Orlando released new concept art, this of the Three Broomsticks pub for the Harry Potter theme park. Summer is a great time to catch up on some reading as the Harry Potter book club started Prisoner of Azkaban, and the book nook reads "To Kill a Mockingbird." Terminus took place in Chicago, and MTV later runs piece on the convention and wizard rock.
August 14, the HPA aired a special podcast One World, One Dream focusing on Olympic/Triwizard goals of unity. The third issue of the Quibbler went live, then on August 16, TLC's updated Half-Blood Prince countdown was made available. PotterCast hit the Big Apple and Detroit with great wizard rock music, and new details regarding the progress at the Wizarding World of Harry Potter appeared online.
New photos from the theme park showed the first snow covered shop in Hogsmeade, where signs will later read "revealed in 2010."
Audio of PotterCast at Comic Con is up, and we also air the special podcast taped live at Terminus, part one and part two.
MTV featured week long coverage of Harry Potter, starting with an article on the Wizard Rockumentary. Our own Melissa Anelli was the subject of a second piece, focusing on her new book Harry, A History. A final article featured various celebs weighing in on why they are Harry Potter fans. A unique display on Harry Potter was featured at the National Library of Medicine, and Wizard Rock the Vote went online. The month ends as Leaky reaffirmed our support of Harry Potter and Banned Books week.
As Banned Book Week continued, Witches Wrock to support Breast Cancer. Opal unveiled completed socks made with new special Harry Potter themed yarn; we like to think Dumbledore would approve. The Noble Collection also revealed new HBP products
including Slytherin's ring, and the locket from the cave. A UK marketing professor said a study proves only Hufflepuffs are loyal to Harry in terms of rereading the book, and following the fandom. October 22 saw a new rumor claiming Hogwarts Castle will not only serve as the center of the Harry Potter theme park, but also house a giant ride. We reminded fans of open registrations for the Harry Potter conventions for next year: LeakyCon 2009, and Azkatraz 2009. PC held a live call out show for debut of new HBP trailer and the Halloween edition of the Quibbler goes live at the end of the month.
Fans heard from Melissa as she held the first reading since publication of "Harry, A History," answers questions about her new book, her interview with Jo and more in New York City. Later Melissa held an event with the We are Wizards theatrical screening. Harry, A History later debuted on the New York Times Bestseller list at number 18, and was featured on the front page of USA Today
Mid November, LeakyCon 2009 opened calls for proposals, and a third trailer hit for Harry Potter and the Half-Blood Prince. Fans react via PotterCast. PC also puts out call for editors. Things are hopping on the lounge with HBP book club readings, crafting for Christmas Ornaments, and winners of a Harry Potter Halloween costume contest we revealed.
November 23 TLC launched our seventh Get a Clue charity fundraiser drive, as Jingle Spells 2 is now available, and nearly 60 percent gone a few days later.
We are Wizards was released on DVD and Leaky and PotterCast released its Harry Potter and the Prisoner of Azkaban DVD Fan Commentary.
Our image galleries team unveiled the 2008 Holiday Image calendar for fans, and the 25th issue of Scribbulus went online. The HP Alliance urged those to Standfast for Darfur, complete with a live call-in program. Leaky and PotterCast announced plans for a Beedle the Bard event in NYC, and a Beedle the Bard book club opened on the Leaky Lounge.
A new poll opened on the Wizarding World of Harry Potter and the first date for the traveling Harry Potter exhibition is revealed: April 30 in Chicago, Illinois. LeakyCon announced our three day lineup of great Wizard rock.
TLC issued a call for programmers, and Jingle Spells 2 became available on iTunes and Amazon. Harry, A History had a holiday event at the Yule Ball in Boston, and the holiday edition of the Quibbler went live.
Written by Sue Upton with assistance from Edward Drogos and Melissa Anelli


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"I love to challenge myself and I always want to fight the best. It motivates me to keep working hard to defeat those challenges."
This was it. The longest title reign in middleweight history was about to come to an end. For four-plus rounds, Chael Sonnen had backed up every ounce of his pre-fight smack talk and was putting the finishing touches on a monumental upset win over Anderson Silva.
There was just one problem – Silva wasn't ready to go yet, and with the seconds ticking away in the fifth and final round, he was still plotting a miracle finish.
"I was thinking of what I had to do to finish the fight, defend my title and go home healthy," said Silva through translator Derek Kronig Lee.
Healthy was out of the question after absorbing a punishing attack from Sonnen, not to mention a pre-fight rib injury, but the finishing the fight and defending the title for a seventh time (tying him with Matt Hughes for most in UFC history), that was still possible. Not probable, but possible, and at the 3:10 mark of the round, less than two minutes away from defeat, he produced a tap out from Sonnen after locking in a triangle armbar.
It was a stirring victory to say the least, one that will remain on Silva's highlight reel forever and a true determinant of his status as an all-time great. Winning easy and in dominant fashion is one thing; winning in the face of adversity is quite another. There would be no Muhammad Ali without the wars with Joe Frazier, no Sugar Ray Leonard without Tommy Hearns, and now Silva had shown that same grit against Sonnen, admitting that when you overcome such a test of wills, the true fighter emerges.
"I think all my fights have been tough, and fighting tough opponents builds a stronger fighter, one who can overcome adversity and stay in the fight to the end."
And even after being beaten for much of the Sonnen bout, it may have been what the Brazilian star needed to break out of the funk that had put a cloud over the last two years of his reign. Sure, he looked spectacular and motivated when jumping up to 205 pounds to blast out James Irvin and Forrest Griffin in less than a round each, but he appeared to sleep walk through defenses against Patrick Cote, Thales Leites, and Demian Maia. Sonnen, in his face before and during their UFC 117 bout, brought the fire back out of Silva.
"I love to challenge myself and I always want to fight the best," he said. "It motivates me to keep working hard to defeat those challenges."
This Saturday night, there will be another challenge to his title, and this one may be the toughest as he faces countryman and former training partner Vitor Belfort in the main event of UFC 126 in Las Vegas.
"He is a dangerous opponent who was a young champion in the UFC," said Silva of his opponent, who won the UFC 12 heavyweight tournament at 19 and then briefly held the organization's light heavyweight title in 2004. "It's going to be a great fight for the fans."
But will it be a great fight for Silva, who is coming off a draining war with Sonnen? You can also add in the pressure of being in his fifth year as champion, something Silva readily agrees can be burdensome at times, but that he seems to have taken control of.
"There is a lot of responsibility and pressure when you are the champion, so keeping a healthy mind is just as important as a healthy body; both aspects are determining in any fight."
Silva has shown his toughness – mentally and physically – on the sport's brightest stage, and at the ritual pre-fight press conference in Las Vegas on Wednesday, the champion made it clear that the Mandalay Bay was his house as he went nose to nose with the challenger. It's added even more intrigue to this early-2011 dream fight, with fans and pundits wondering if the seemingly unbeatable "Spider" can hold off the charge of the still explosive "Phenom."
The champion admits that Belfort "is a tough opponent" who "has good hands and has a lot of experience," but when asked how the challenger has progressed as a fighter over the years, Silva's response is simply "I'm not sure, we will have to wait and see on February 5th."
It's as much as you'll get out of the champion, who saves his charisma for the Octagon and for those moments when the microphones are put away. But if his body language this week in Vegas says anything, it's that his come from behind win over Sonnen has lit a fire under him that now has him approaching every fight with the intention of taking over from the opening bell. That's a scary proposition for opponents, but a compelling one for his fans, who Silva promises to entertain in 2011.
"I want to defend my belt and fight the best," he said. "The fans can expect to see great fights this year."


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The Bugaboo Donkey latest addition to Bugaboo's portfolio and is set to become the new king of pavement. Built to grow with your family, the Bugaboo Donkey targets a growing niche is the ever-changing world of premium strollers. Whether you've got one child, two children, or even twins, this one stroller is all you need! 3 simple clicks allow the Bugaboo Donkey to change from a mono (single-mode) to a new full-size duo stroller and back just as easily.
Whether you're off to the shops or the park, the Bugaboo Donkey is an ideal solution. Its innovative side luggage basket and large underseat basket means there's enough storage space for your shopping or a day out and about – with easy access to both – your child can stay sound asleep in the bassinet or reclinable seat. Extend the side luggage basket and bring even more goods by converting the stroller into the duo configuration. Large swivel wheels and the one-piece handlebar guarantee maneuverability in places you'd never imagine you could go. With the duo extension set converting to a duo configuration comes with great ease. This makes the Bugaboo Donkey future proof.
Flexible and multiple luggage options
One-piece fold and self standing
5-point harness
Compatible for one or two car seats
Two-wheel position
Modular system
One-hand operating seat recline and reverse
Height-adjustable handlebar
Suitable for infants to toddlers
Tires - air-filled 10" swivel wheels and 12" rear wheels
Large water-repellent sun canopy
Machine washable fabrics
Suitable for children up to 37.5 lbs.
Maximum width: mono configuration 23.6", duo/twin configuration 29"
Weight: mono configuration: 29 lbs duo/twin configuration: 33lb
Weight Capacity of Storage: up to 12 gallons
Includes the rain cover!


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AGPA ELISA Kit
- Target Alle AGPA Produkte
- AGPA (Anti-Glycoprotein Antibody (AGPA))
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 1.563-100 ng/mL
- Untere Nachweisgrenze
- 1.563 ng/mL
- Applikation
- Verwendungszweck
- The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GPAb in human serum, plasma and other biological fluids
- Proben
- Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This kit recognizes natural and recombinant Human GPAb. No significant cross-reactivity or interference between Human GPAb and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between Human GPAb and all the analogues.
- Kreuzreaktivität (Details)
- No significant cross-reactivity or interference between human Anti-Glycoprotein Antibody and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Human bFGF and all the analogues, therefore, cross reaction may still exist.
- Sensitivität
- 0.938 ng/mL
- Bestandteile
Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis - Benötigtes Material
Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer
- Applikationshinweise
ELISA Plate: The just opened ELISA Plate may appear water-like substance, which is normal and will not have any impact on the experiment results.
Add Sample: The interval of sample adding between the first well and the last well should not be too long, otherwise will cause different pre-incubation time, which will significantly affect the experiment's accuracy and repeatability. For each step in the procedure, total dispensing time for addition of reagents or samples to the assay plate should not exceed 10 minutes. Parallel measure ment is recommended.
Incubation: To prevent evaporation and ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps. Do not let the strips dry at any time during the assay. Strict compliance with the given incubation time and temperature.
Washing: The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings. Residual liquid in the reaction wells should be pat dry against absorbent paper in the washing process. But don't put absorbent paper into reaction wells directly. Note that clear the residual liquid and fingerprint in the bottom before measurement, so as not to affect the micro-titer plate reader.
Reagent Preparation: As the volume of Detection Ab and HRP Conjugate is very small, liquid may adhere to the tube wall or tube cap when being transported. You better hand-throw it or centrifugal it for 1 minute at 1000rpm. Please pipette the solution for 4-5 times before pippeting. Please carefully reconstitute Standards, working solutions of Detection Ab and HRP Conjugate according to the instructions. To minimize imprecision caused by pipetting, ensure that pipettors are calibrated. It is recommended to suck more than 10μL for once pipetting. Do not reuse standard solution, working solution of Detection Ab and HRP Conjugate, which have been diluted. If you need to use standard repeatedly, you can divide the standard into small pack according to the amount of each assay, keep them at -20°C to -80°C and avoid repeated freezing and thawing.
Reaction Time Control: Please control reaction time strictly following this product description!
Substrate: Substrate Solution is easily contaminated. Please protect it from light.Stop Solution: As it is an acid solution, please pay attention to the protection of your eyes, hands, face and clothes when using this solution.
Mixing: You'd better use micro-oscillator at the lowest frequency, as sufficient and gentle mixing is particularly important to reaction result. If there is no micro-oscillator available, you can knock the ELISA plate frame gently with your finger before reaction.
Security: Please wear lab coats and latex gloves for protection. Especially detecting samples of blood or other body fluid, please perform following the national security columns of biological laboratories.
Do not use component from different batches of kit(washing buffer and stop solution can be an exception)
To avoid cross-contamination, change pipette tips between adding of each standard level, between sample adding, and between reagent adding. Also, use separate reservoirs for each reagent. Otherwise, the results will be inaccurate! - Kommentare
Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).
Information on reagents:
Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.
Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration. - Probenmenge
- 100 µL
- Plattentyp
- Pre-coated
- Protokoll
- This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to GPAb. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antigen. Then a biotinylated detection antigen specific for GPAb and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GPAb, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of GPAb.You can calculate the concentration of GPAb in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
- Aufbereitung der Reagenzien
Bring all reagents to room temperature (18-25°C) before use.
Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer into 750 mL of Wash Buffer with deionized or distilled water. Put unused solution back at 4°C. If crystals have formed in the concentrate, you can warm it with 40°C water bath (Heating temperature should not exceed 50°C) and mix it gently until the crystals have completely dissolved. The solution should be cooled to room temperature before use.
Standard: Prepare standard within 15 minutes before use. Reconstitute the Standard with 1.0 mL of Sample Diluent, let it stand for 10 minutes until it dissolved fully. This reconstitution produces a stock solution. Then make serial dilutions as needed (Making serial dilution in the wells directly is not permitted). The Sample Diluent serves as the zero (0).
Biotinylated Detection Ab: Calculate the required amount before experiment (100μL /well). In actual preparation, you should prepare 100-200μL more. Centrifuge the stock tube before use, dilute the concentrated Biotinylated Detection Ab to the working concentration using Diluent for Biotinylated Detection Ab (1:100).
Concentrated HRP Conjugate: Calculate the required amount before experiment (100μL /well). In actual preparation you should prepare 100-200μL more. Dilute the Concentrated HRP Conjugate to the working concentration using Diluent for Concentrated HRP Conjugate (1:100).
Substrate Reagent: As it is sensitive to light and contaminants, so you shouldn't open the vial until you need it! The needed dosage of the reagent can be aspirated with sterilized tips and the unused residual reagent shouldn't be dumped back into the vial again.
Note: please don't prepare the reagent directly in the Diluent vials provided in the kit. Contaminated water or container for reagent preparation will influence the result. - Probennahme
Samples should be clear and transparent and be centrifuged to remove suspended solids.
Serum: Allow samples to clot for 2 hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000×g. Collect the supernatant and carry out the assay immediately. Blood collection tubes should be disposable, non-pyrogenic, and non-endotoxin.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2 - 8°C within 30 minutes of collection. Collect the supernatant and carry out the assay immediately. Hemolysis samples are not suitable for ELISA assay!
Other biological fluids: Centrifuge samples for 20 minutes at 1000×g at 2 - 8°C. Collect the supernatant and carry out the assay immediately.
Tissue homogenates: You'd better get detailed references from other literatures before assay aiming at different tissue types. For general information, hemolysis blood may affect the result, so you should mince the tissues to small pieces and rinse them in ice-cold PBS (0.02M, pH=7.4) to remove excess blood thoroughly. Tissue pieces should be weighed and then homogenized in PBS (the volume depends on the weight of the tissue) with a glass homogenizer on ice. To further break the cells, you can sonicate the suspension with an ultrasonic cell disrupter or subject it to freeze-thaw cycles. The homogenates are then centrifugated for 5minutes at 5000×g to get the supernate.
Samples should be used within 7 days when stored at 2-8°C, otherwise samples must be divided and stored at -20°C (≤ 1month) or -80°C (≤ 6months) to avoid the loss of bioactivity and contamination. Avoid repeated freeze-thaw cycles.
Please take the samples to room temperature (18-25°C) without extra heating before performing the assay.
Please predict the concentration before assaying. If the sample concentration is not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments. - Testdurchführung
Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. It's recommended that all samples and standards be assayed in duplicate.
1. Add Sample: Add 100μL of Standard, Blank, or Sample per well. The blank well is added with Reference Standard & Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 90 minutes at 37°C.
2. Biotinylated Detection Ab: Remove the liquid of each well, don'tn'tn't wash. Immediately add 100μL of Biotinylated Detection Ab working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C.
3. Wash: Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 350μL) (a squirt bottle, multi-channel pipette, manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, remove remained Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper.
4. HRP Conjugate: Add 100μL of HRP Conjugate working solution to each well. Cover with the Plate sealer. Incubate for 30 minutes at 37°C.
5. Wash: Repeat the wash process for five times as conducted in step 3.
6. Substrate: Add 90μL of Substrate Solution to each well. Cover with a new Plate sealer. Incubate for about 15 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but not more than 30minutes. When apparent gradient appeared in standard wells, user should terminate the reaction.
7. Stop: Add 50μLof Stop Solution to each well. Then, the color turns to yellow immediately. The order to add stop solution should be the same as the substrate solution.
8. OD Measurement: Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters.
9. After experiment, put all the unused reagents back into the refrigerator according to the specified storage temperature respectively until their expiry - Ergebnisberechnung
Average the duplicate readings for each standard and samples, then subtract the average zero standard optical density. Create a standard curve by plotting the mean OD value for each standard on the y-axisagainst the concentration on the x-axis and draw a best fit curve through the points on the graph. It is recommended to use some professional software to do this calculation, such as curve expert 1.3 or 1.4. In the software interface, a best fitting equation of standard curve will be calculated using OD values and concentrations of standard sample. The software will calculate the concentration of samples after entering the OD value of samples. If samples have been diluted, the concentration calculated from the standard curve must be multiplied by the dilution factor. If the OD of the sample surpasses the upper limit of the standard curve, you should re-test it after appropriate dilution. The actual concentration is the calculated concentration multiplied dilution factor.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
- Handhabung
- All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
- Lagerung
- Informationen zur Lagerung
- The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
- Target Alle AGPA Produkte
- AGPA (Anti-Glycoprotein Antibody (AGPA))
- Andere Bezeichnung
- Anti-Glycoprotein Antibody (AGPA Produkte)
- Substanzklasse
- Antibody


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Mohawk Laminate Flooring Photos
About Mohawk Laminate Flooring
Beautiful Decorator Patterns - Mohawk Laminate Flooring is designed to enhance the beauty, warmth and style of your home.
Installs Anywhere in the Home - Mohawk Laminate Flooring can be installed almost anywhere in the home, including over dry concrete slabs, wooden subfloors, as well as many types of existing floors.
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Easy To Clean - the CrystalShield™ Finish is easy to clean and never needs waxing. Wipe away dirt with a moist cloth; for tough stains use Acetone™ (nail polish remover).
Scratch & Scuff Resistant - Mohawk Laminate Flooring is more resistant to scratching and scuffing that most other types of hard surfaces floors.
Hygienic - Mohawk Laminate Flooring is non-allergenic and does not absorb odors. It's the healthy choice.
Fade Resistant - Mohawk's decorator layer won't fade and change color from ultra violet sunlight.
Lasting Value - Mohawk Laminate Floors are backed by a Lifetime Structural Warranty.


text:
Promotional apparel is the largest and best selling category of promotional products available. Why? Think about all the places you see promotional apparel. Custom promotional apparel gets used in offices as corporate apparel for employees, companies use custom apparel to achieve better brand recognition, and organizations imprint apparel with their logo to raise money for a charitable cause.
The bottom line is that no matter where you look, chances are you will see some sort of imprinted apparel. IASpromotes.com stocks a large selection of promotional apparel that is easy to imprint with your logo. Our custom apparel offers you the perfect promotional items that you need to promote your brand.


text:
This report provides information as reference resource for your personal consumption only. It is not intended to be and does not constitute
financial advice, investment advice or any other advice. This report has been generated without regard to the individual financial
circumstances, needs or objectives of persons who view it. Users should not rely on any of the information herein as
authoritative or substitute for the exercise of their own skill and judgment in making any investment or other decision. By the use of our services, you agree
that any and all use of this report which you make, is solely at your own risk and without any recourse whatsoever to Square Foot
Research Pte. Ltd., its related and affiliate companies and/or their employees. Square Foot Research Pte. Ltd. makes reasonable effort to use reliable and comprehensive information to generate this report, but we make no
representation that it is accurate or complete.


text:
NDBM_File - Tied access to ndbm files |
NDBM_File - Tied access to ndbm files
use Fcntl; # For O_RDWR, O_CREAT, etc. use NDBM_File;
# Now read and change the hash $h{newkey} = newvalue; print $h{oldkey}; ...
untie %h;
establishes a connection between a Perl hash variable and
a file in NDBM_File format;. You can manipulate the data in the file
just as if it were in a Perl hash, but when your program exits, the
data will remain in the file, to be used the next time your program
Use NDBM_File
with the Perl built-in tie
function to establish
the connection between the variable and the file. The arguments to
should be:
. (Ths tells Perl to use the NDBM_File
package to perform the functions of the hash.)
The name of the file you want to tie to the hash.
Flags. Use one of:
If you want to create the file if it does not exist, add O_CREAT
any of these, as in the example. If you omit O_CREAT
and the file
does not already exist, the tie
call will fail.
On failure, the tie
call returns an undefined value and probably
sets $!
to contain the reason the file could not be tied.
ndbm store returned -1, errno 22, key "..." at ...
This warning is emmitted when you try to store a key or a value that is too long. It means that the change was not recorded in the database. See BUGS AND WARNINGS below.
There are a number of limits on the size of the data that you can store in the NDBM file. The most important is that the length of a key, plus the length of its associated value, may not exceed 1008 bytes.
See tie in the perlfunc manpage, the perldbmfilter manpage, the Fcntl manpage
NDBM_File - Tied access to ndbm files |


text:
Medellín B&W No 1 Poster
Made on demand in Gemany.
Shipped out within 1-4 business days.
Free DE shipping on orders over €49
The Medellín B&W No 1 Poster is the perfect touch for any room as a trendy and personal piece of décor. This print is created to be on display, impressing everyone who comes into your home.
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© Nbourhood
All our orders are custom-made in the USA to meet your needs.
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Free Germany shipping on orders over €49.
All orders are made on demand to order to ensure you receive a perfect print. Please allow 2-7 business days for your order to be delivered. We take great pride in our prints and package them with the utmost care to ensure they arrive safely and in excellent condition.
As all our orders are custom-made and on-demand, each poster is printed specifically for you. Unfortunately, this means we are unable to accept returns or exchanges. Customized items, by law, are not entitled to a refund. Additionally, frames are not eligible for returns.
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