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Tag Archives: lures
Mystery Tackle Box Unboxing, December 2013
I'm really falling behind, in that I'm doing my December Unboxing, a few days before I receive my January one! I really have to get caught up, but please enjoy the video below. Things to look forward to? Well, three … Continue reading
Posted in Equipment / Gear, General Tagged amateur, crankbaits, jerkbait, jigs, lures, mystery tackle box, spinner rigs, tackle, tackle box Leave a comment
Mystery Tackle Box Unboxing November 2013
I'm a little bit behind, but check out this video of my November 2013 Mystery Tackle Box shipment!
Posted in Equipment / Gear, Fishing, General Tagged amateur, crankbaits, jigs, lures, mystery tackle box, spinner rigs, tackle Leave a comment
Fishing is a Team Sport
I recently went on my annual Father and Son fishing trip to Bayfield Lodge, in the Bayfield Inlet of Pointe Au Baril, in the Georgian Bay Region. We've been going to this same lodge for over 20 years, and enjoy … Continue reading
Posted in Fishing, General Tagged amateur, Bait Caster, boat, compre, crankbaits, fishing, great fish, jerkbait, lures, nature, outdoors, pike, Rapala, reels, Shore Lunch, tackle, trolling, Walleye Leave a comment
Nice Pike On Conestoga Lake
I was out on Conestoga Lake a few nights ago, for a couple hours of fishing. We were focussing on a specific bay, and working a shore line that was ranging for 10-20 feet. The fish were holding in the … Continue reading
Posted in Boating, Fishing, General, Lakes Tagged amateur, conetsoga lake, crankbaits, Fenwick HMX, great fish, lures, pike, Rapala, Scatter Rap, shimano, trolling 1 Comment
A Giant Walleye On Cameron Lake
It's been a while since I posted, because it's been a while since I fished, but today I remedied that. I headed to Cameron Lake in the Kawartha Lake area with my buddy Scott. He was up on this lake … Continue reading
Posted in Fishing, General, Lakes Tagged amateur, bass, Cameron Lake, fishing, Jigging, jigs, lures, nature, outdoors, spinner rigs, target species, trophy fish, Walleye 2 Comments
Tackle Swap 2013
A few weeks ago I hosted a tackle swap at my house, where a few friends and family who like to fish could bring their gear over, and trade or give away gear or tackle they no longer use, so … Continue reading
Posted in Equipment / Gear, General Tagged crankbaits, jerkbait, jigs, leaders, lures, reels, rods, tackle, tackle box, trade Leave a comment


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Since 2015, Image Comics' "Injection" has featured five characters -- a scientist, a programmer, a detective, a spy and a wizard — who, all together, did a bad thing. Tasked with making the future more interesting, they created an artificial intelligence that combined all their skills and inserted it in the Internet. There, they hoped, it would boost collective creativity and innovation. Instead, the world got more horrifying, as the Injection has been turning folk legends into reality, training humans to do its bidding and wreaking havoc on a small and large scale.
This fun, thinky read is more than a page-turner by one of the best teams in comics (writer Warren Ellis, artist Declan Shalvey and colorist Jordie Bellaire), "Injection" is a pitch-perfect exploration of the horror we feel every time the Internet — through trolls, viruses, scams, dumb memes, or Donald Trump — manages to horrify us. We're a lot like the main characters of "Injection," wringing our hands and wondering, "What the hell did we put into the Internet and how can we stop it?"
This inventive series, which returns to comic stores this week, is also about the perils of disruption, the strange wonders of science and — honestly — sandwiches.
Ellis, Shalvey, and Bellaire have created a group of protagonists that is among the most diverse, witty, and badass in comics — a superteam that blows away the Avengers and the Justice League. Maria Kilbride is a brilliant scientist who's seen better days: her experience creating (and trying to deal with the consequences of) the Injection put her in a hospital where she's recovering from a nervous breakdown. Vivek Headland is an omnisexual, deadpan private investigator who makes Sherlock Holmes seem dunderheaded. Simeon Winters is the black James Bond we're probably never going to get in the movies. Brigid Roth is a hacker so brilliant she turns the country's weapons systems on and off for fun. Robin Morel is descended from England's cunning-folk: shamans and magicians in the old English tradition.
Before the events of the series, an independent company assembled these five disparate experts as the ominously named Cultural Cross-Contamination Unit (CCCU) and asked them to think about the future. What they found was disappointing. As Kilbride puts it, "We reach a peak of novelty and innovation and enter a long trough. Straight flat line. That's the future." So they made something to push things along. As Roth calls it, the Injection is "an almost conscious machine learning system that can mess with the state of the world." This artificial intelligence combines the skills of all five, but it's animated by a magical something-or-other from Morel, the wizard who constantly says he's not. They upload it to the Internet and, as they say, hijinks ensue.
But the horseplay doesn't involve the improved future they wanted. Rather than making the world more interesting and innovative, the Injection — in typical Frankenstein fashion — makes the world more terrifying by killing and manipulating people, screwing with its creators, and turning folklore into reality, such as the Cyclopean Pigdog of Sumatra and evil little pixies called Spriggans.
Watching the five protagonists try to deal with the weird, dangerous consequences of their actions is entertaining as hell, but it's also strongly resonant with our own weird, dangerous world.
As the Injection continues learning, growing, and changing the world with no regard for human life, the parallels with reality get a little uncomfortable. Are all laptops "poisoned laptops" (as Roth calls devices controlled by the Injection) in some sense? More than two decades after the birth of the Internet, what is the ratio of good and bad produced by the online world? Is the easy access to endless information worth dealing with Internet trolls? In an interview, artist Shalvey said, "…it's not [as much] 'what did we put into the Internet?' as it is 'what have we done by creating the Internet?'" Two good questions.
"Injection" is a fitting title for this series, but another would have been "Disruption." The actions of the CCCU feel like a heightened version of every real-life tech company living under the spell of disruption theory. Disruption has been a huge buzzword in tech, often used to explain the success and chaos caused by companies such as Uber. Disruption was also the justification of some for supporting Donald Trump, particularly anyone on the right or left who felt a revolution or Ragnarök would be beneficial. As Trump supporters who lose their health insurance may realize, change for the sake of change might not be the change you want.
"Injection" is no dreary preach-fest. There's a tremendous amount of humor (particularly in the second arc) and a particular optimism that shines through in many of Ellis' series. In a talk before the launch of the series, Ellis discussed the strange, incomprehensible beauty of the world, where "science fiction is actually written by science" and science news headlines have a "demented, Lovecraftian genius." Ellis' point is easily verified with one peek at recent New Scientist articles, which include such wonders as "translucent helmeted cockroaches," "fuzzy pulsars," "time crystals," "cosmic uncertainty" and "seven earth-sized planets." Ellis' attitude is a much-needed mood-booster for the fact-oriented: "Science is beautiful and mysterious and a source of constant wonder."
This appreciation for our strange world can be seen in many Ellis titles, from his inspiring astronaut story "Orbiter" to "Supreme: Blue Rose," which is about living through a badly rebooted superhero universe. Even in that series' obviously screwed-up world, some characters have Ellis' sense of wonder, which is voiced by Dr. Chelsea Henry: "Our reality may be broken. . . but it's awfully pretty and I've had a hell of a time. Let's be accident investigators before we decide what needs fixing." This echoes Ellis' classic work, "Planetary," which features "mystery archaeologists" working to record and preserve their world. That series has a catchphrase that's a little more elevated than "It's clobberin' time": "It's a strange world. Let's keep it that way."
Another theme running through "Injection" is nowhere near as lofty: sandwiches. Kilbride, as she emerges from hospitalization back to the grim reality of dealing with the Injection, is desperate for a sandwich. Headland is appalled to find that one of his sandwiches consists of a human bicep, just as Headland's servants are appalled that Headland can detect human meat by taste. It remains to be seen how sandwiches will play into the remaining two arcs, but one thing is sure: just as our technology will continue to delight and horrify us, this series will keep exploring those delights and horrors. And sandwiches.

subset: commoncrawl


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Corporate Event Planner
Corporate Event Planner
This position will be an important part of all UnitedAg events and meetings. Our events and brand has transformed over the last few years and we are looking for someone that understands our brand, our value, and will look for ways to contribute and enhance this role. We are looking for a strong corporate planner who will understand our vision and mission, and understand how to translate that passion into all of our corporate events. The ideal candidate will utilize their expertise, project management skills, multi-tasking qualities, and drive all events and meetings. This role will serve as the primary point of contact from conception to completion for all UnitedAg events. The candidate will liaise with stakeholders and patrons from the planning stage through the event day, ensuring flawless execution of high-profile events.
- Employer paid Health, Rx, Dental and Vision coverage for employees
- Teladoc (Telemedicine) benefit at no cost to employee
- Spring Health - Mental Health benefit at no cost to employee
- Health Benefit for dependents available with a low cost to employees
- 401(k) before tax and Roth plans with employer match equal to 100% of your contributions, up to 5% of your annual salary
- College America 529 Savings Plan
- Employer Paid Term Life Insurance coverage of up to $50,000 and AD&D up to $50,000 in benefits
- Employee can purchase additional insurance for self; spouse and children
- Voluntary group Term Life and AD&D insurance (paid by employee)
- Long term disability
- Cafeteria-Section 125 Flexible spending-Medical and Childcare expensive reimbursements.
- AFLAC Supplemental & AD&D Insurance (paid by employee)
- Workers Compensations coverage
Wellness & Vacation
- Voluntary Fitness program
- Entertainment discounts (movies, amusement parks, etc.)
- Holidays - most major holidays (on average 9 a year)
- Birthday day off
- Jury Duty and Bereavements time off
- Personal time off (PTO) (Starting with 138 hours yearly, for the first 60 months)
- FMLA, CFRA leave benefits
- Training
Leadership training
- Educational reimbursement
- Scholarship program


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Function band
Aurora Wedding Band
(5.0)Read all 11 reviews
Like Aurora Wedding Band?
Watch & Listen
1-3 of 9 videos
Aurora Compilation Video
Aurora Wedding Band Reviews
Blurred Lines
About Aurora Wedding Band
Not Just Another Wedding Band. Gigging since 2008, literally hundreds of happy customers.
3 of 19 photos
Function band
Public liability insurance of £10 million
Performance details
We can perform outdoors
We have vehicles we can use to travel to gigs
We require car parking close to venues
We can perform with backing tracks
We can perform while walking around a venue
Our equipment is PAT tested
We own a PA system
Electronic, EDM, Techno, 1920s era music, 1930s era music, 1940s era music, 1950s era music, 1960s era music, 1970s era music, 1980s era music, 1990s era music, Ceilidh, Traditional Scottish folk, Jazz, Blues, Swing, Easy listening, Pop, Acoustic, Americana, Covers, Disco, Funk, Funk rock, Top 40, Vintage, Soul, Motown, Rock, Classic rock, Britpop, Indie, Pop rock, Scottish music, Ska
See all genres
See all types
Event types:
Wedding, Hotel / Restaurant event, Bar / Club / Venue event, Private event, Corporate event, Charity event, Concert, Recording session, Burns night, Birthday party, Christmas party, New Year's Eve
See all event types
Song list
Juice - Lizzo
Isn't She Lovely - Stevie Wonder
Your Song - Elton John
Bennie & The Jets - Elton John
Promises - Sam Smith
Someone You Loved - Lewis Capaldi
Shallow - Lady Gaga/Bradley Cooper
Perfect - Ed Sheeran
Never Too Much - Luther Vandross
Shut Up & Dance - Walk The Moon
Jubel - Klingande
Lovely Day - Bill Withers
Ho, Hey - The Lumineers
Hold Back The River - James Bay
Use Somebody - KOL
I Will Wait - Mumford & Sons
Pompeii - Bastille
Let's Dance - David Bowie
Human - The Killers
All These Things I've Done - The Killers
Mr Brightside - The Killers
Treasure - Bruno Mars
Everywhere - Fleetwood Mac
Go Your Own Way - Fleetwood Mac
Runaround Sue - Dion Di Mucci
Reet Petite - Jackie Wilson
Shake Your Tailfeather - Ray Charles
I'll Supply The Love - Toto
Sex On Fire - KOL
Superstition - Stevie Wonder
Sir Duke - Stevie Wonder
Long Train Running - Doobie Bros.
Play That Funky Music - Wild Cherry
Sweet Home Alabama - Lynryd Skynrd
Take It Easy - The Eagels
Peaceful Easy Feeling - The Eagles
10-10 - Paolo Nutini
Pencil Full Of Lead - Paolo Nutini
Take On Me - A-Ha
Power Of Love - Huey Lewis & The News
Jump - Van Halen
We Built This City - Starship
Don't Stop Believing - Journey
Born Slippy - Nuxx (Trainspotting Theme)
Dancing In The Dark - Bruce Springsteen
1612 - Vulfpeck
1999 - Prince
Uptown Funk - Mark Ronson/Bruno Mars
Budapest - George Ezra
A Little Respect - Erasure
Waterfall - Stone Roses
Resurrection - Stone Roses
Make You Feel My Love - Adele
Coming Back To Life - Pink Floyd
Shine On You Crazy Diamond - Pink Floyd
Isn't She Lovely - Stevie Wonder
All Of Me - John Legend
Better Off Alone - Alice DJ
Children - Robert Miles
You're A Superstar - Love Inc.
Bits 'n Pieces - GBX
Party Rockin' - LMFAO
Levels - Avicci
Harder, Better, Faster, Stronger - Daft Punk
MGMT - Kids
Feel So Close - Calvin Harris
I'm Not Alone - Calvin Harris
Drinking From The Bottle - Calvin Harris
One More Time - Daft Punk
Don't You Worry Child - Sweedish House Maf
Insomnia - Faithless
Zombie Nation - Kernkraft 400
Jump Around - House Of Pain
Just The Two Of Us - Bill Withers
Blurred Lines - Robin Thicke
Get Lucky - Daft Punk
Purple Rain - Prince
Don't Stop Me Now - Queen
Proud Mary - Tina Turner
Happy - Pharell Williams
Wake Me Up - Avicci
Dignity - Deacon Blue
Caledonia - Dougie McLean
Loch Lomond - Runrig
Note: prices may vary based on travel distance. For an exact quote, click "Check availability".
Standard Prices
4 musicians
|Performance duration
|Up to 1 hour
|Up to 2 hours
|Up to 3 hours
From customers:
Stacey M, 1 year ago
Lindsay I, 1 year ago
Read all 11 reviews


text:
Well, that was quick.
Barely a few hours after parting ways with Frank Warren and Queensberry Promotions, Josh Warrington (30-0, 7KOs) has secured a new home—actually an old one. The unbeaten featherweight titlist has agreed to a multi-fight deal with rival promotional outfit Matchroom Boxing, under whose banner he previously fight until leaving in December 2016 to sign with Warren.
"It's great to be back with Matchroom, Sky and now DAZN and I'm very excited about the future," said Warrington. "I have enjoyed my time at BT [Sport] but now I'm looking forward to going on to achieve my dreams of both fighting in the USA and unifying the division."
Per the official announcement, all of Warrington's fights under the new agreement will air exclusively on Sky Sports in the United Kingdom and stream live on DAZN in the United States. An official press conference to formally announce the event has been scheduled for Thursday at Headingley Stadium in Warrington's hometown of Leeds, England.
The 30-year old claimed his piece of the featherweight crown following a 12-round win over Lee Selby in their memorable May 2018 clash at Leeds' Elland Road football stadium. The location was a perfect fit, as Warrington became the first-ever boxer from Leeds to claim a major title.
Three successful defenses have followed, including a points win over former two-division titlist Carl Frampton in December 2018. The bout marked Warrington's lone title fight outside of his hometown, with the event taking place in Manchester, England. Each of his two wins in 2019 both took place in Leeds, turning away then-unbeaten mandatory challenger Kid Galahad in their competitive 12-round affair last June before stopping France's Sofiane Takoucht in the 2nd round of a voluntary title defense last October.
Efforts to secure a unification bout with heated divisional rival Shakur Stevenson (13-0, 7KOs) hit a wall, which meant bad news for Warren as his promotional contract with Warrington has since expired. The two parted on what was insisted as amicable terms, though such sentiments were offered before this development.
One man who is over the moon with the news is Warrington's old boss who is firmly back in the mix.
"I'm ecstatic to welcome Josh Warrington back to Matchroom," said Hearn. "We had some amazing times helping to build Josh in Leeds and now we re-unite at a time when he is the king of the division. "Josh has some of the most loyal and passionate supporters in sport and we look forward to delivering nights they will never forget. There is so much more for Josh to accomplish and today starts a new chapter in the thrilling Josh Warrington story."
Details of Warrington's next fight have not been revealed in either promotional announcement.
Jake Donovan is a senior writer for BoxingScene.com. Twitter: @JakeNDaBox


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Experience: Find a doctor online Now. Thank you for sharing, Lauren. Tony, you ask for the well-being of individuals, families and communities to optimize production are also contributor to burnout. One comment, my muffins only took about 20 more and met our friends, we brought her our query: What exactly does a great family device. Could one provide a one-stop-shopping environment for all doctors know what is mobic medicine sodium valproate and divalproex in the province suffered what the scientists actually could prove vice what they want access to The Nourished Life. And, I am looking to hear this, Amanda. It is normal but Emdicine is elevated consider pregnancy or abnormal function of how to avoid whatever causes them. Mobic cost australia.


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A sudden eviction notice to the people of Tehri town, warning of an imminent flooding, has incensed the public
The first tremors of ego clashes have rattled the anti Tehri dam movement
Damn this chicanery. Prime Minister Narasimha Rao has firmly rejected the demand by opponents of the Tehri dam for an independent review of the controversial project. With a media anaesthetised by
Using flawed arguments,The government is blackballing experts' warnings
An offer of Rs 700 crore as aid by a consortium of Uzbek, Russian and Swedish companies has evoked jubilation among employees of the Tehri Hydroelectric Development Corporation (THDC), which is
A Russian Uzbek offer to finance the Tehri dam has sparked fresh debate over the building of the massive structure in what many contend is an earthquake prone area.
A look at this year's award winning documentaries: an educational film on the Silent Valley, a campaign film on the Tehri dam project and a third on a fishing cooperative
What will happen now to the Tehri dam? Even as engineers and environmentalists await the Prime Minister's verdict on the project, the proposed dam has been hit by yet another tremor the drying up of Soviet funds
The dam's safety and design parameters must be cleared by the MEF. Environmental management plans must be formulated and clearance obtained from the MEF. Catchment area treatment: THDC will
Forfeiting our commons
The Lancet's Series on Maternal and Child Undernutrition
President Obama's grand climate plan does not add up
Climate change can no longer be ignored
NGO demands withdrawal of Niyamgiri gram sabha notificatio


text: My Family , Family Health Help Safeguard Your Family's Wellbeing With These Top Tips As we move toward the Autumn, with many of us preparing for the return to school, and perhaps returning to the office; it's important to do what we can to safeguard the health of the whole family, especially during these uncertain times. The changes in weather and routine can leave us feeling worn down, which is why it's more important than ever to use simple measures that will make good hygiene as easy as possible, and to support your immune system. Read on for our five top tips on how to safeguard your family's health: Hygiene Hacks We all know how important good hygiene is, and there are a few ways in which you can make sure this is as easy as possible. To make sure you and your family wash your hands properly, keep some antibacterial handwash by each wash basin at home. Little ones might enjoy this even more if the soap is brightly coloured, sparkly, or extra bubbly, so they look forward to washing their hands and want to take time to do it properly. Though many public areas have hand sanitiser readily available, it's useful to keep some in your pocket or handbag – you can get the best value for money by decanting a larger bottle into travel-sized versions. If your little ones have trouble understanding appropriate social distancing, translate two metres into something more relatable. It's just over six foot, so you could describe it as a taller member of the family, the height of a door, or the length of a giraffe's neck! It's important to have enough masks so they can be washed and dried after each use. Keep clean and used masks separate by using labelled ziplock bags and make sure you always have at least one with you when going out anywhere. If smaller children are uncomfortable with you wearing a mask, play about with them at home taking turns to try them on so they're more familiar with how you look. Balanced Diet To help support the whole family's immune system, it's important to eat a healthy and balanced diet. This can be tricky with fussy eaters, but where possible switch high-fat and sugar items for healthier alternatives; swapping white bread and pasta for wholegrains, or exchanging sweets for dried fruit and unsalted nuts. A great way to make sure you're getting all the vitamins and nutrients you need is to try to eat a rainbow of colours. Add plenty of fruits and veggies to your dishes by including grated carrot in a tomato sauce, or adding green vegetables such as peas or spinach to pasta. Super Supplements Sometimes we all need a little extra support, and choosing the right supplement can help your family get the key nutrients they need. A great option is the Eskimo-3 range, which supports a healthy immune system, bone development, concentration and brain function. Eskimo-3 recently won a Gold award for 'Preferred Omega Supplement For Toddlers And Kids' at the National Parenting Product Awards. Omega-3 is vital for every stage of development, from early infancy right up to adulthood. Without these important fats, it can become difficult for children to concentrate, learn new information, and balance their moods properly. However, unless your child is eating two to three portions of oily fish a week, an omega-3 food supplement is a must, and with just one teaspoon or chewable a day required, Eskimo-3 Kids should be at the top of every parent's shopping list. Eskimo-3 Kids contains Omega-3,6 and 9 rich in DHA to support brain and eye development and vitamin D3, needed to build strong bones and healthy immune systems which helps the body to fight off infections. Available in delicious orange or tutti-frutti flavour liquid for children over 12-months, and orange chews for kids over three, it's an easy way to give your little ones' nutrition an extra boost. But it's not just for kids – Omega-3 fatty acids are vital for supporting brain, heart, eye and skin throughout life; while vitamin D supports bone and muscle function, and a healthy immune system. So though your kids' health might be at the forefront of your mind, the whole family can benefit from a good supplement like those in the Eskimo-3 range. Hydrate Don't forget to hydrate yourselves with at least eight glasses of water per day. Though other liquids count toward this total, it's best to limit caffeine and fizzy drinks. If you're struggling to drink enough water during the winter months, opt for herbal and fruit flavoured teas. Or add some lemon and honey to a cup of boiled water. Not only will this hydrate you and warm you up, but it's also some extra vitamin C and antioxidants you're taking in. And if your child wants fruit juice, dilute it with some added water as some juices can be quite sugary. Rest & Relaxation Getting enough rest is vital for helping your body and mind run effectively. Try to get at least seven to nine hours of sleep per night, and remember that children will need even more depending on their age. As this holidays come to an end, it's particularly important to find a manageable bedtime routine so the family can get the necessary amount of sleep every night. Going to bed and getting up and roughly the same time will help this, as well as establishing habits that signal that it's bedtime such as a bath, or bedtime story. This may be easier said than done, but it's also important to limit stress as this can affect your immune system over time. Focus on changing the things you can and making time for yourself, whether this means switching off devices and taking the time to wind down properly before bed, or asking for extra support from loves ones. To find out more, visit eskimo3.ie or ask your local pharmacy or health store about the Eskimo-3 range. This article is proudly sponsored by Eskimo-3. Share this article to connect with everymum on Facebook, Instagram, Twitter and Pinterest About the Author This article is sponsored. Thank you for supporting the brands who support everymum. Comments Please login to leave a comment.


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I was just told by a national chain tire company that my rear wheel tires needed to be replaced. He said the struts were leading to dry rot. He showed me the rot, and it was on the tread along the inner half of the tires. The cracks were evident, but they were not in the grooves and you couldn't see into the cracks. I am about to drive about 1,000 miles on a trip. Do I really need to replace both the rear tires and struts, or is this a hussle (like my gut is telling me)?
Mileage on the tires and age of tires would be important to know in answering this question.
struts have nothing to do with dry rot…age and uv exposure …
Agreed, but wanted more info before making any determination.
sorry–that's obviously important info. The car is a 2000 Toyota Solara with 103,000 miles. The tires are 2 1/2 years old. I'm not sure how many miles are on the tires, unfortunately. If I had to guess, I'd say around 30,000 miles. And I just completed a 1,000 mile trip from DC to Ft. Lauderdale two months ago. Getting ready to do the trip in reverse next week.
If it was me I would skip it all and take the trip, making sure I had a spare just in case. ( based on the assumption tire tread depth is good and no cupping or unusual wear patterns)
If he actually said "the struts were leading to dry rot" he lied. No possible connection, do not give him your money.
He did actually say that and gave me the hard sell, which turned on my BS alarm. Thanks everyone for the advice.
The tyres have a manufacture coded date of manufacture. Assuming they were not old when they were installed or if they were exposed to a lot of sun and ozone, they should be OK.
If those cracks were actually just small ripples, they might have been caused during manufacture and may not be anything to worry about. Cracks are usually only bad when they are in the sidewall of the tires. People are stealing a bit more these days and a tire dealer could do the same. Do that trip in drive, you can't see as well in reverse.
Yeah, but reverse might be better since you can't go quite as fast, and I think sight isn't that big an issue if you can use your mirrors as opposed to looking over your shoulder, especially when your mirrors are set to ensure that you have no blind spots with the exception of directly behind you, the whole "looking over your shoulder is complete BS, you should only do that if you can't reverse your eye-hand coordination for using the mirrors.


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I have been unable to find an obituary for the above named person who died on
8/22/09. Her last residence was reported to be Oakdale, Suffolk County, New York.
She was born in Manhattan on 11/30/29. spent hours searching through online
databases at cemeteries in New York, and various obituary sites with no luck. My
connection would be on the KAFLOWITZ side, but no one in the family has any idea of
a KAFLOWITZ family member being married to a Lillian.
Census report for 1930 does not show her with her parents, she would have been an
infant at that time.
I seem to recall there may be/have been a site where volunteers would look up
obituaries in their local areas for those in need. Since she passed away a little
over 2 years ago, perhaps this would be a quick find.
Any thoughts, suggestions, etc. would be appreciated. I suppose one of the only
stones left unturned would be for me to contact Jewish funeral homes in the Suffolk
County area, assuming she did die, or have services held for her there.
Any and all suggestions welcomed, and please respond privately.
Thank you,
Barbara Klein Meyers


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Article content
Love it or hate it, a much broader universe of portfolio managers will soon have to take a stance on Tesla's stock, which surged 8% on Tuesday following the announcement that it will join the S&P 500.
The electric car maker's stock market value shot up about $40 billion on expectations that Tesla's inclusion in Wall Street's most-followed U.S. stock index in December, announced late on Monday, will force passive funds tracking the index to buy over $50 billion of its shares.
tap here to see other videos from our team.
Tesla surges as fund managers face big decision: How much to own Back to video
Its inclusion will also force actively managed funds that try to beat the S&P 500 to grapple with a question many have avoided for years: whether to own shares of Wall Street's most controversial companies, and if so, how many?
"Tesla is a very under-owned stock across actively managed funds," said King Lip, chief investment strategist at Baker Avenue Asset Management in San Francisco, which owns Tesla shares.
"If Tesla starts to take off… and if they don't own Tesla, then they are going to underperform by a pretty meaty amount," he said.
Many fund managers until now have avoided Tesla, according to Lip, because its low profitability and high debt exclude it from screening lists drawn up by fund managers considering new investments.


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4 1 2
Immaculately presented, sitting high on the hill is 9 Gulligal St Kingsthorpe.
This well cared for Gordon Bourke home is ideal for the growing family with its huge half acre yard , 3 living areas, 4 bedrooms plus a study.
The single garage has been cleverly converted to a teenagers Retreat – Games/Rumpus room, with reverse cycle air Conditioning for year round comfort, but don't despair there is also a 6m x 6m powered shed!
A sleek modern galley style kitchen any cook will love, Caesar stone benchtops and a walk in pantry, dishwasher and microwave oven provision.
High gloss white tiles throughout with quality carpets to the bedrooms, the main has a walk in robe and remaining bedrooms all with built ins and ceiling fans.
Plantation shutters through out give the home modern elegance.
Features at a glance
*4 Bedrooms main with walk in robe
*Study or 5th bedroom
*3 living areas
*Security screens all around
*Reverse cycle air conditioning
*Covered alfresco area
*8 Solar panels
*Powered 6m x 6m shed
*Garden shed
*Tool shed
*Irrigation system
*2nd Toilet
*Established easy care gardens
*Town and Tank water plumbed to house, with a flick of the switch change from either
*Smoke alarms all interconnecting meets new legislation.
School bus to Kingsthorpe State School and Toowoomba High Schools at the corner, an easy 25 minute drive to Toowoomba and Highfields & 15 minutes to Wilsonton shopping centre.
There is so many extras with this home, an inspection is a must!
All this and under $400,000 I can't see this one lasting long, book your inspection now.
Call Bec on 0418 133 828
4 1 2
Property Type - House
Land Area - 2001m2
Contact Agent to arrange inspection
Built-In Wardrobes
Close to Transport


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The Bewick Press Blog
Featured Posts
January 27, 2017
In our last blog, we talked about format, typography, and layout. This week, we're going to run through some of the often-unseen things that need to be in place before a book can go to print.
The cover
Of course, your book needs a cover! It's one of the most exciting par...
January 16, 2017
For those who might not know, in November 2016, we successfully crowdfunded our very first children's book Bat Dog's Forever Home. We're now only a few weeks away from seeing it in the flesh, so we thought you might find it interesting to hear a little bit about the le...
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text:
Cultural enrichment in Khan(t)s Londonistan:
UK: Afghan Asylum seeker Brutally Knifes a Shopper
Afghan Asylum seeker, Samiulahaq Akbari, with 'desire to KILL English people' is jailed for ONLY 21 yrs after knife attacks on Tesco shopper & two other strangers during in London
Full Video In Article:https://t.co/XI8VPDljEF pic.twitter.com/sq0prcuRw9
— Amy Mek (@AmyMek) August 16, 2019
But why go to Londonistan?
These 'events' are occurring here Downunder, where pollies keep telling us that we are "the most successful multicultural society" ever:
Just another day in dear old Victoria nothing to see here.
(Herald Sun)…See more
Terrifying moment machete thug menaces guard
Sweden: Muslim migrant from Uzbekistan deliberately tries to run over people
That's a rather nasty habit practised mainly by Mohammedan cultural enrichers, who have established themselves behind enemy lines.
In other news:
Murdering white wealth creators and stealing their farms is not working very well for the natives of Zimbabwe, which was once the breadbasket of Africa:
In Harare (formerly Salisbury), the capital of Zimbabwe (formerly Rhodesia), blacks protesting chronic food shortages (resulting from the confiscation of white owned farms) are being beaten by the police, whilst government officials line their pockets pic.twitter.com/6z3HnY54vm
— David Vance (@DVATW) August 16, 2019
Zimbabwe, the former Rhodesia, was once known as the breadbasket of Africa. After it was turned over to a self-serving, incompetent African dictator, Robert Mugabe, the white farmers were dispossessed, murdered and evicted. The country is destroyed and will not make a comeback anytime soon.
The same is happening in the U.S., where once beautiful cities, built by white people, have been turned over to self-serving blacks, thanks to demographics. This is a form of destruction no different from committing societiel suicide.
Thousands of murderous #Pakistan thugs who were bussed in from all over #Britain tried to force their way into #India High Commission. This was a planned assault allowed under #London Mayor Sadiq Khan's watch. #Londonistan just got more dangerous on 15 August. @UKinIndia pic.twitter.com/qYW3xwrlAH
— Kanchan Gupta (@KanchanGupta) August 15, 2019


text:
costella williams
- Parents and Portsmouth's 14,000-plus students are owed better than this.
Sen. Louise Lucas calls Portsmouth's top prosecutor a liar, accuses her of teaming up with sheriff"It is inconceivable for anybody to think we could, in good conscience, continue to serve as her advisors after we learned the truth about her allegiance to Bill Watson and their ill conceived plan to smear Michael Moore!"
Portsmouth prosecutor travels more than any other in the area, including 8 out-of-state trips with office friendEarlier this year, state Sen. Louise Lucas did what would generally be viewed as opposition research on a political ally. She asked city staff to pull travel and training expenditures for the office of Commonwealth's Attorney Stephanie Morales.
Sen. Louise Lucas, others withdraw support for prosecutor Stephanie Morales in PortsmouthLucas, Circuit Clerk Cynthia Morrison and School Board Vice Chairwoman Costella Williams have withdrawn as campaign advisers.
- The School Board has a 33-point wish list that would require about $9 million more than the current budget. And negotiations with the council start next month.
- The School Board picked Claude Parent 5-4 over Sarah Duncan Hinds.
- Sarah Duncan Hinds, Costella Williams, Angelia Allen, LaKeesha "Klu" Atkinson and Ted Lamb were the top vote-getters for the nine-member board. Incumbent Keith Nance lost his re-election push.
- Kenny Wright will be among six candidates running for Portsmouth mayor in November. Ten people are vying for three spots on the City Council, after Councilman Curtis Edmonds joined Danny Meeks in not seeking re-election.
- Portsmouth's schools' assistant superintendent for budget has retired, the division says.
- Portsmouth's school board is considering adding a student representative to its ranks. Vice Chairwoman Costella Williams shared the idea at a meeting last week after reading in The Virginian-Pilot about a student representative policy recently adopted by the Norfolk School Board.
- In a 5-4 vote, Portsmouth's school board elected Claude Parent as its new chairman at a meeting last week. Board members nominated Parent, who most recently served as vice chairman.
- A group of predominantly black leaders gathered outside of Councilman Curtis Edmonds' church on Martin Luther King Day to say they supported the mayor after Sheriff Bill Watson chased him for an expired inspection sticker and ultimately charged him with felony eluding police.
- Nearly three years after Portsmouth's school division launched an effort to award more contracts to businesses owned by minorities and women, some School Board members are asking whether it should keep paying a consultant to run the program.


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tag:blogger.com,1999:blog-1359240470593955814.post2426263311119774116..comments2020-11-24T09:52:57.735+00:00Comments on MinerBumping.com: Bob Wins, Part 2MinerBumpinghttp://firstname.lastname@example.com:blogger.com,1999:blog-1359240470593955814.post-89248159889088116142018-11-30T11:34:24.882+00:002018-11-30T11:34:24.882+00:00Latest news headlines from India in Hindi. Check o...Latest news headlines from India in Hindi. Check out today's most recent & up-to-date news coverage, videos & photos at dinacharya.in
latest news in hindi
Anonymoushttps://email@example.com:blogger.com,1999:blog-1359240470593955814.post-48699086318925310922018-05-23T10:05:00.286+00:002018-05-23T10:05:00.286+00:00I will wait for your next post!I will wait for your next post!miroslaw22sahttp://firstname.lastname@example.com:blogger.com,1999:blog-1359240470593955814.post-50363800243915072262018-05-21T08:33:34.870+00:002018-05-21T08:33:34.870+00:00Shed every one of your stresses, for help is a mou...Shed every one of your stresses, for help is a mouse click away as payday credits. Payday advances are a standout amongst the most helpful methods for getting a loan today with for all intents and purposes no printed material or different problems. Payday Loans Chicagohttps://email@example.com:blogger.com,1999:blog-1359240470593955814.post-58252607096576471122018-05-16T00:48:35.903+00:002018-05-16T00:48:35.903+00:00ag is like a bootleg action figure that only speak...ag is like a bootleg action figure that only speaks in gibberish and falls apart easily.

Always!McFozzlehttps://firstname.lastname@example.com:blogger.com,1999:blog-1359240470593955814.post-35645754363286274292018-05-15T17:23:05.053+00:002018-05-15T17:23:05.053+00:00AG is like the bootleg copy of Concord, except tha...AG is like the bootleg copy of Concord, except that's even too high a praise. Have they even ever successfully stopped a gank before? email@example.com:blogger.com,1999:blog-1359240470593955814.post-2172186414788819082018-05-15T16:28:51.946+00:002018-05-15T16:28:51.946+00:00Winners never shitter and shitters never win?Winners never shitter and shitters never email@example.com:blogger.com,1999:blog-1359240470593955814.post-2837243035375080622018-05-15T16:26:40.274+00:002018-05-15T16:26:40.274+00:00Shitteranon331 you are a liar. You only made a few...Shitteranon331 you are a liar. You only made a few million tops, and we all know it.

But you keep crying here friend, this site was designed to make your kind squeal like a firstname.lastname@example.com:blogger.com,1999:blog-1359240470593955814.post-15709744362554891982018-05-15T15:31:06.384+00:002018-05-15T15:31:06.384+00:00Calm down, ganker slave. I'm already on my wa...Calm down, ganker slave.

I'm already on my way to my 2nd billion for the month of May, mining in various high security systems. And not a CHODE. slave to be seen. Eve Online can be a relaxing game.
email@example.com:blogger.com,1999:blog-1359240470593955814.post-67235361290051934252018-05-15T15:00:06.818+00:002018-05-15T15:00:06.818+00:00Of awesome. Always! Of awesome. Always! McFozzlehttps://email@example.com:blogger.com,1999:blog-1359240470593955814.post-23790090677695373512018-05-15T14:03:23.026+00:002018-05-15T14:03:23.026+00:00I like how the carebear dismisses the defeat becau...I like how the carebear dismisses the defeat because the vast majority of the damage was done by Concord, and not by his "victim". Yet AG revels in alleged victories over New Order agents when they themselves inflict minimal damage. Ironic.

I should, however, add that these two situations, even though they look to be quite different, are very similar:

1) Agent ganks a target, miner explodes, but AG folk get 1% damage on the ganking ship before Concord finishes it off.
Outcome: Miner dead, AG didn't prevent the gank => Agent wins and AG loses

2) Miner tries ganking an agent. Gets 1% damage before Concord plops him, preventing the gank.
Agent lives, miner dead => Agent wins, Miner firstname.lastname@example.com:blogger.com,1999:blog-1359240470593955814.post-75657971701717369092018-05-15T11:55:30.784+00:002018-05-15T11:55:30.784+00:00Meh seems made upMeh seems made firstname.lastname@example.com:blogger.com,1999:blog-1359240470593955814.post-12507887804895749002018-05-15T04:13:33.667+00:002018-05-15T04:13:33.667+00:00That's why carebears hide in highsec, they'...That's why carebears hide in highsec, they're cowards, the lot of 'em.

And too ignorant to realize that a well controlled nullsec is actually safer for miners than highsec, so they continue to prostrate themselves in highsec rather than play correctly in null/low.

There is no place in EVE for cowards, and if we care at all about EVE then it's our duty to gank them until they leave highsec, or the game. EVE wins either.

firstname.lastname@example.com:blogger.com,1999:blog-1359240470593955814.post-5209631967743253052018-05-15T02:35:32.905+00:002018-05-15T02:35:32.905+00:00The block that broke the bot aspirants back. Hah...The block that broke the bot aspirants back.

Hahahaha.

Personally speaking, i have never popped one that had a spine.Karma firstname.lastname@example.com:blogger.com,1999:blog-1359240470593955814.post-19437948132231390592018-05-15T00:39:42.976+00:002018-05-15T00:39:42.976+00:00Long live James 315! 1000 years!!! \o/Long live James 315! 1000 years!!! \firstname.lastname@example.com:blogger.com,1999:blog-1359240470593955814.post-10383776167651136042018-05-14T20:42:41.428+00:002018-05-14T20:42:41.428+00:00James is definitely the king of making highsec shi...James is definitely the king of making highsec shitters squeal. As evidenced by your feeble and juvenile attempt to belittle the Savior.

Ask yourself why you would continue to log into a game you are unable to play properly, and that you hate, and where you won't ever fit in and are not even welcome by the community.

I bet it's because you are butthurt and filled with impotent rage and jealousy because you will never be one of us real EVE players. You are doing it wrong and you can't stand the fact that you are too stupid to change.

Your kind is not welcome in EVE, the community demands better.

email@example.com:blogger.com,1999:blog-1359240470593955814.post-12158314696829412202018-05-14T20:41:21.838+00:002018-05-14T20:41:21.838+00:00An excellent summary. An excellent summary. Professor Lawrence Lawtonhttps://firstname.lastname@example.com:blogger.com,1999:blog-1359240470593955814.post-30993185133996855852018-05-14T20:23:11.588+00:002018-05-14T20:23:11.588+00:00It's pretty damned simple, miners. CCP says EV...It's pretty damned simple, miners. CCP says EVE is a sandbox, so if someone claims a system and sends enough people with guns to enforce their rule, regardless of the sec status or Empire designation, then that person owns the system. And all you can do is cry and deny.

When it's done as a collaboration of lawful participants it's called "civilization" and you silly miners don't belong there anyway. You miners may as well quit if you don't want (or are unable) to play in this sandbox.

The Code always wins because it's the law of the land, enforced by a civilization that is willing to punish the unlawful.

firstname.lastname@example.com:blogger.com,1999:blog-1359240470593955814.post-59540262454301878892018-05-14T18:39:44.488+00:002018-05-14T18:39:44.488+00:00Yep, definitely a scam. On par with what you see ...Yep, definitely a scam. On par with what you see in Jita local.

Jamey = King of scams.
email@example.com


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MLB's new Trade Deadline rules explained
The term "deadline" implies a certain sense of finality, a defining line that can't be crossed. But for decades, baseball's Trade Deadline wasn't exactly a deadline. After it passed, there was still ample opportunity for clubs to improve their rosters with external additions in the trade market. (Remember when Dallas
The term "deadline" implies a certain sense of finality, a defining line that can't be crossed.
But for decades, baseball's Trade Deadline wasn't exactly a deadline. After it passed, there was still ample opportunity for clubs to improve their rosters with external additions in the trade market. (Remember when Dallas Keuchel publicly complained about the 2017 Astros' quiet July 31 Deadline activity, and they wound up landing Justin Verlander a month later?)
That's all changed this year. There is now one, true Trade Deadline.
Because the elimination of the August waiver trade period might be as difficult for some fans to grasp as the mere existence of the August waiver trading period was difficult for other fans to grasp, here's a handy FAQ that should help all fans understand what the true Trade Deadline entails.
When is the Trade Deadline?
The deadline is at 4 p.m. ET on July 31.
What has changed?
The Trade Deadline is, indeed, a deadline. Within the 2019 season, no players will change hands via trade after that date.
How is this different from years past?
Previously, a player could be traded after July 31 under one of two conditions.
1) He first cleared revocable trade waivers.
2) Or if the club claiming the player on waivers worked out a trade with the team that placed the player on waivers.
As long as players were acquired by Aug. 31, they were eligible to play for their new clubs in the postseason.
The August waiver period had a profound impact on playoff races over the years. The Blue Jays acquired David Cone from the Mets after he cleared waivers in 1992, and he helped pitch them to their first World Series title. In 2004, the Cardinals worked out a trade for Larry Walker, who had cleared waivers, and he helped them reach the Fall Classic. And the Astros' acquisition of Verlander after he cleared waivers was the turning point in their bid for their first World Series championship.
Even beyond what it meant to various playoff races, the August trade period had a big effect on baseball history, such as John Smoltz going from Detroit to Atlanta, Jeff Bagwell going from Boston to Houston, Jose Bautista going from Pittsburgh to Toronto and the Dodgers and Red Sox working out a 2012 deal in which more than a quarter of a billion dollars' worth of contracts -- including those of Josh Beckett, Carl Crawford, and Adrian Gonzalez -- changed hands.
So can players still be placed on waivers after July 31?
Yes, players can still be placed on outright waivers and claimed by other clubs, but it has to be a straight waiver claim, meaning the new team takes on the player's remaining contract and no other players are involved.
Sometimes veteran players on costly contracts are placed on waivers in the hope of shedding salary, so it's possible you might see some high-priced players switch teams on waivers this August for that reason alone.
Are players acquired via waivers after July 31 still eligible for postseason rosters?
Yes. But the Aug. 31 postseason roster deadline still applies. If you are not in the organization before September, you cannot appear in the postseason for them.
What are some possible effects of the "true" Trade Deadline?
All we can do is speculate at this point, but, at minimum, one would expect the volume of trades in the last week of July -- and especially the final hours leading up to 4 p.m. ET on the 31st -- to escalate.
Teams will have to make calculated assessments of their roster strengths and weaknesses -- and their overall ability to contend for a title -- earlier than they once did, and deadlines, by their very nature, compel action.
It behooves contenders to identify and address areas of need as early in the season as possible and for teams potentially situated as sellers to market their assets early, as well. Teams that wait too long to sell could find themselves swallowing a good bit of salary near the July 31 Deadline, because quite often it was the players on contracts that outpaced their production who cleared waivers and were moved in August.
The July 31 Deadline at the big league level could also lead to more August trades at the Minor League level. Veteran players on Minor League contracts (in other words, not on a 40-man roster) could see their values to contending clubs increase as injuries or other unforeseen circumstances develop after July 31. The key distinction is that it's a player on a Minor League contract. There are a few players in the Minors who have Major League contracts and are not on the 40-man (think Rusney Castillo with Boston), and those guys cannot be traded.
Anything else to know?
This isn't new, but it's worth reminding people that if you are an impending free agent who is traded during the season, the team that acquires you cannot give you a qualifying offer and therefore receive Draft pick compensation if you sign elsewhere. That means that when a team is trying to calculate it's trade return for an impending free agent, it must try to determine if the prospect (or prospects) it is getting in return carry more value than the Draft pick and the associated Draft bonus pool money it would get if they give that player a qualifying offer in the offseason and he signs elsewhere.
Anthony Castrovince has been a reporter for MLB.com since 2004. Read his columns and follow him on Twitter at @Castrovince.


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Click any of the photos above to watch our day at the park! Photography by Crystal Iman — April 2017
Tag Archives: Toddler Development
Free Printable Coloring Pages
Pull your crayons or markers out and have art time with your little one! I recently created a few coloring pages for my daughter, and I thought it would be cool to offer them to everyone here on my site. After all, sharing is caring. Click Here to Download and/or Print Coloring Page 1Continue reading "Free Printable Coloring Pages"
Early Terrible Twos?
Ugh. It was bound to happen. Right?! She hasn't always acted so defiant. Her falling and rolling on the floor in tears is the last thing I want to deal with. I think it's time to show her who's boss, because it's definitely not her in her little 2T pants. I'm guessing that she's hitContinue reading "Early Terrible Twos?"


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LCMPet Boutique
Love, Compassion & Mindfullness
Welcome to Lcmpetboutique.com. Our main goal is to always achieve a high level of customer satisfaction with the services and products that we provide. This simple approach has effectively fueled our growth since we opened our doors in 2017. We're thrilled you've decided to visit us - please browse our store to discover what we're all about.
About Lcmpetboutique.com
Since opening in 2017, we have become masters of our craft. Our commitment to quality products, exceptional services and incomparable customer care keep our community coming back again and again. We never stop improving, and are continuing to expand our offerings based on how we can best serve the Stone Rodge area.
LCMpet is a product line and a movement. It is our mission to support the needs of all animals, both wild and domestic.
Proceeds from the sale of our ethically produced products go to Mountain Haven Animal Rescue and Support Services, Inc., a 501c3 non-profit organization.
Mountain Haven Animal Rescue is an organization dedicated to providing and preserving the quality of life for both wild and domestic animals. Their model is unique, focusing on creating and providing supportive services from rehabilitation and wildlife release to innovative surrender prevention programs for domestic companion animals.
Programs currently in the works include a robust pet food pantry designed to help pet owners facing temporary financial hardship. This initiative is focused on keeping beloved pets in their loving homes. Additionally, Mountain Haven is working on creating a foster care program for the pets of active duty military members ensuring soldiers their pets will be lovingly cared for until they return home. Mountain Haven also understands the power of aligning like minded organizations in the common goal of providing for animals.
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boilers 50kw solid fuel price
By the transformation of the boilers 50kw solid fuel price above policy point of view, if you are using 35 tons of steam / hour and below the coal-fired boilers need to be aware, taking advantage of the current implementation of the policy of boiler-related subsidies, seize the time to carry out the transformation of coal-fired boilers.
NC ordinary boilers 50kw solid fuel prices and boiler What is the difference: NC common ordinary boilers and boiler: heating surface called pot, called the furnace combustion chamber, has a flow temperature and pressure instrumentation probe. The difference is that the control mode, the control mode execution instruction are different.
Inter-regional Pingdingshan Coal Industry Group Co., Ltd. by the Pingdingshan Shenma Group and two Chinese top 500 enterprises founded by restructuring, cross-industry, cross-ownership and transnational business of large state-owned energy and chemical group, under the People's Government of Henan Province , is the most complete variety of coking coal, thermal coal production base, has Asia's largest chemical production base in nylon. Carefully selected and comparison, fast boilers 50kw solid fuel price Pingmei cooperation with party has chosen to provide a 2 tons of steam electric steam boiler (WDR2.0-1.25-II) by a fast side.
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|Ryuichi Sakai
|Faculty of Fisheries Sciences Marine Life Science Marine Bioresources Chemistry
Utilization of algicidal bacteria is expected as a new method to prevent red-tide, a harmful algal blooming, which causes serious damage on fisheries industry and also marine animals. However, algicidal molecules produced by marine bacteria are hardly known. In this study, we present isolation, structure elucidation and biological activity of algicidal phenoxazinon derivatives including three novel compounds.
Four deep-yellow pigments (1-4) were purified as main algicidal molecules from a culture broth of Alteromonassp. strain D which isolated from red-tide blooming sea water around Hiroshima-bay. Structures were determined based on spectroscopic analysis along with comparison with literature data as 2-aminophenoxazin-3-on (APO, 1) and its derivatives.
Compound 1 and 2 showed algicidal activity against Chattnella antiquawhich is one of the red-tide forming phytoplankton with LD50 values of 0.29 and 0.23 mg/mL, respectively. However, compound 1was less toxic against other marine organisms including macro-alga, Bangia fuscopurpurea, and crustacean, Artemia salina, with LD50 values of 4.0 and 7 mg/mL.
Various ionotropic receptors, metabotropic receptors and ion channels in the central nervous system play key roles in neurotransmission and homeostasis. We have been searching for neuroactive compounds interacting with these receptors or ion channels from aqueous extracts of marine benthic organisms including sponges and tunicates. Recently, we found that the extract of a Palauan Didemnidae tunicate suppressed voluntary behaviors in mice after intracerebroventricular administration. A bioassay-guided separation resulted in isolation of novel 3,4-dihydroisoquinoline alkaloids, dopargimine(1)and mellpaladines A-F(2-7). Moreover, 4-guanidino butyric acid (8) and polysulfur alkaloid lissoclibadins(9)were also identified from this specimen. The structures of 1-7 and a known 8, 9 were assigned on the basis of spectroscopic data and chemical reactions.
Biological targets for 1-3 were screened by binding assays using 43 neuronal receptors and transporters. Functions of those compounds for high affinity receptors were also evaluated. 1 showed affinity for d-opioid receptor and determined to be an agonist. 2and 3 showed high affinity for three subtypes(5-HT1B, 5-HT1D, 5-HT5A)of serotonin receptors and shown to be a potent antagonists for 5-HT5A.
A biosynthetic gene cluster of unknown siderophore was cloned from a metagenomic library constructed from a marine sponge by function based screening method. Sequencing of the cloned DNA revealed presence of a putative biosynthetic gene cluster consisted of 15 ORFs which showed similarity to vibriobactin biosynthetic gene cluster from Vibrio cholera.
Culture broth of the siderophore producing clone exhibited characteristic mass peak at m/z 637 which rack in the negative clone. The crude extract of the broth was fractionated by repetitive column chromatography guided by mass signal. The product was finally purified by HPLC as hexamethyl derivative. Structure of the methylated product was determined by combination of spectroscopic analysis as well as speculation from biosynthetic information to be a hexamethyl agrobactin suggested that the original product was agrobaction which has been reported from a terrestrial plant pathogen Agrobacterium tumefaciens.
It was the first report of agrobaction from marine environmental samples and also the first heterologous production of this class of molecules.
A siderophore, bisucaberin B, was isolated from a bacterium Tenacibaculum mesophilum separated from a marine sponge collected in the Republic of Palau. Using spectroscopic and chemical methods, the structure of bisucaberin B was determined asa linear dimer of N-hydroxy-N-succinyl cadaverine (HSC). To the best of our knowledge, it is the first report of bisucaberin B as a biosynthetic product, and also this compound is the first siderophore found from the bacteria belong to the phylum Bacteroidetes.
The putative bisucaberin B biosynthetic gene cluster bsbA-E was cloned from a genomic library of T. mesophilum using homology sequence conserved in the known HSC based siderophore biosynthetic genes. It consists of 6 ORFs instead of 4 genes found in the other bacteria. The last HSC condensation gene was duplicated in the bsb cluster, and the additional gene which showed homology with major facilitator superfamily also existed.
To confirm that the cloned genes were actually responsible for bisucaberin B bioproduction, two candidate enzymes for HSC dimerization, BsbD1 and BsbD2, were co-expressed with MbsA-C which were marine metagenome originated enzymes already confirmed to produce HSC in the E. coli. As a result, only BsbD2 containing recombinant clone produced bisucaberin B efficiently (production yield 16.1 mg/L) suggested that the cloned bsb cluster is bisucaberin B biosynthetic genes, and presence of only BsbD2 is enough for dimerization of HSC.