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Monday, 24 May 2010
Sunshine from Sita and Dr Ravi Zacharias
Posted by Amrita at Monday, May 24, 2010
9 Fertilize my soul:
- Holly, the Old Western Gal said...
You are a sunshiny person, that is for sure! Literally, too, with the hot sun taking the temps into unreasonable triple digits!!!
- 24 May 2010 at 22:47
- Felisol said...
A man who can serve the truth with a laughter, is bound to be a success.
- 25 May 2010 at 05:34
- Sita said...
Girl...I'm pretty sure many of your bloggy friends can sing for you.."You are the sunshine of my bloglife...that's why I'll always come around..."
Ravi's sister is my pastor's wife and she has the same brilliant sense of humour!
- 25 May 2010 at 06:15
- Terry said...
dear amrita..i didn't know that dr ravi zacharias was born in india!
amrita, mom golden really loves him and his preaching.
we get him on the radio wdcx and he is listened to by so many canadian christians.
this video here is just something!
have you ever met him in real life?
now THAT would be something!...hug your little mama for me and give sheeba a pat on the head!...love terry
- 25 May 2010 at 08:44
- Amrita said...
Dear Holly, I treasure your gentle talk, artistic graphics and most of all your friendship.Visiting you cheers me up so much my dear.
Dear, you said rightly, wisdom delivered with humour makes a deep impression.
Oh no Sita, I don 't deserve that. It is such a privelledge to know Ravi 's family.He must have visited your church too.
Hi Terry, Dr Zac is a great precher. I have some of his messages on tape. When I was grade 8 I went to my first YFC youth camp in Calcutta where he was one of the speakers. In our church we had a conference arranged by his ministry.
I shall pass on your greeting to Mama and Sheebs.
- 25 May 2010 at 09:32
- Julia Dutta said...
Ravi Zacharias is amazing Amrita. I really think God speaks through him. However, one must have the ears to hear it :)))
- 25 May 2010 at 13:00
- Nadwrażliwiec said...
Sita is right! Your blog is worth of awards :) God bless You Amrita :D
- 25 May 2010 at 18:12
- Amrita said...
That 's right Julia Dr Zacharias is a wonderful speaker, he makes his point well. Julia. Have have another video of his - the perception of beauty.
Thank you Zimb.
- 25 May 2010 at 19:18
- Grand Junction Chiropractic said...
great videos! :)
Chiropractic Grand Junction
- 1 June 2010 at 02:03


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Priced at around $400 without any storage, the new ReadyNAS NV+ v2 is slightly cheaper than the older but higher-end ReadyNAS Ultra 4 while offering higher storage capacities and more features. Where it needs to catch up, however, is its performance: though not slow, the NV+ v2 was significantly slower than the Ultra 4 in our testing.
To make up for that, the ReadyNAS NV+ v2 sports easily front-accessible drive bays, and USB 3.0 connectivity, and its flexible X-RAID2 configuration makes replacing and upgrading the server's internal storage a nonissue. The server also has a great backup mechanism and supports Apple's Time Machine. On top of that it now features Netgear's sleek new RAIDiator 5 operating system for NAS servers. While easier to use than its predecessor, RAIDiator 5 doesn't add many more features, however, and the server is still behind competitors' servers in this regard, missing popular options such as PC-less download and support for IP cameras.
If you're looking for a relatively affordable server with lots of storage space, and don't mind the intermittent loud noises its fan emits, the ReadyNAS NV+ v2 is a decent choice. For better performance and more features, also check out the Synology DiskStation DS410.
Design and setup
ReadyNAS NV+ v2 has almost exactly the same design as the ReadyNAS Ultra 4: it's as compact as a four-bay NAS server can be. On the front the server has a large door that opens to reveal the hard-drive trays, which can be pulled out easily without using any tools. Once a hard drive is installed, a tray can be locked into its place firmly with a latch. You do need a screwdriver and four proprietary screws to attach a hard drive to a drive tray, though fortunately the server comes with enough of them for all four drives.
Below the drive bay door, there's a small LCD that shows the IP address of the NAS server and the current status of the internal hard drive. On top of the drive bays are a USB 2.0 port and a Backup button that quickly copies a USB drive's contents into the server's internal storage. On the back, the server has two USB 3.0 ports to host more storage via external hard drives. The NV+ v2 is the first NAS server we've seen that supports this new and much faster USB standard.
Unlike the Ultra 4, which comes with two Gigabit Ethernet ports, the NV+ v2 has only one. This means it doesn't have the fail-safe or load-balancing networking feature, which is not a terrible shortcoming, since most NAS servers don't have that, either.
The NV+ v2 comes with a CD of help materials and a desktop application called RAIDar you can use to detect the NAS in the network, browse its share folders, and launch its Web interface to further customize the server's features. For the server's target audience, tech professionals, the NV+ v2 is easy enough to figure out.
The reviewed unit comes with one 1TB hard drive but the server can handle up to four hard drives of up to 3TB each (as opposed to the 2TB of the Ultra 4) for a total maximum storage space of 12TB. (In most cases, when all four bays are occupied with 3TB hard drives, you'll get about 9TB of storage out of the server; generally the other 3TB are used for redundancy overhead.) The server, however, works even when there's only one hard drive installed.
Regardless of how many hard drives you use, even if it's just one, the server by default will set them up in Netgear's proprietary X-RAID2 configuration. Similarly to Synology's Hybrid RAID or LaCie's AutoRAID, X-RAID2 enables you to dynamically expand the volume without having to back up or restore the existing content. The configuration automatically safeguards the setup against a single-drive failure. Obviously, you'll need at least two hard drives for the XRAID 2 to be effective. In this case the two drives will be set up in a RAID 1-like configuration (also called a "mirror," in which only half of the total capacity is available). Now if you add another hard drive, the volume will be dynamically expanded and changed to a RAID 5-like setup, which is balanced between data integrity and maximum storage space. And you can keep doing that until the bays are all occupied.
X-RAID2 permits changing the hard-drive setup anytime, even when the server is running, as long as you only add or subtract one drive at a time. You can even replace an existing drive with another of larger capacity, making this a great way to upgrade the server's storage without having to rebuild the RAID from scratch. Note, however, that once a new hard drive is added, it takes a very long time for it to be synced with the existing RAID. This means, depending on the capacities of the drives, it could take up to a few days for you to replace all four drives. The good news is the server still works, though at slower speed, during this process and you can keep track of this process via the front LCD.
The ReadyNAS NV+ v2 doesn't have much to offer in terms of features. Nonetheless, the server does come with a completely new user interface compared with the Ultra 4. It's sleeker and more or less resembles the UI of an operating system. Once logged in you'll be greeted with the Dashboard showing the server's operating condition. On top, you'll find four self-explanatory tabs, Configure, Documentation, Community, and How To. The Configure tab is, obviously, the most important since it takes you to all the server's settings.
The server offers an easy way to add or remove users and share folders. Once each new item is added, you'll be able to assign the access privileges (Read-only, Read/Write, No access) of each user to each folder with a few clicks. For each share folder, you can also turn on the ReadyDLNA feature, which permits streaming digital content stored in that folder to DLNA-compliant streamers in the network. All in all, we found the new interface much easier to use than the Ultra 4's.

subset: commoncrawl


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The Brisbane EV Festival and Conference is on in October...
The Brisbane AEVA Branch is busy preparing for the EV Conference to be held on Friday 28th October and the EV Festival, to be held in King George Square, Brisbane, on the 29th of October.
Our Friday Conference line-up has almost been finalised with some very exciting headliners, including speakers from a variety of backgrounds such as battery technology, car manufacturers and government departments. It will be exciting to learn about the future of Electric Vehicles in Australia! This event includes morning tea, lunch and afternoon tea, and is free to our members, with registration a must!
Whilst some members have already agreed to display their electric vehicles at the Festival, we are still requiring more owners who are willing and able to promote their vehicle and the group. As well as members' home built vehicles, there will be a large number of commercial displays from charge points to production vehicles, from major manufacturers, as well as displays from similar interest groups (e.g. ATA).
We are eager to get as many members either displaying and/or attending the conference. If you would like to attend, please contact me firstname.lastname@example.com or phone me 0414 736 253 (please leave a message if I don't answer, or call me after 6pm).
As with all things, there are registrations to be filled in asap! We have some Brisbane members willing to billet some interstate members, so get in quick to secure your spot!
Annual Conference and Festival Bris
For Australian Capital Territory Members of the AEVA
Forum rules
All may view this forum, only AEVA Members may post here.
All may view this forum, only AEVA Members may post here.
Post Reply
1 post • Page 1 of 1
Annual Conference and Festival Bris
Treasurer of the Brisbane AEVA. Meetings are the 3rd Wednesday of the month at the Albion Peace Centre!
Post Reply
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Accenture, global professional services company providing strategy, consulting, digital, technology and operations services, has partnered with Erasmus Student Network to bring to our members and other international students a bridge to high quality job positions.
Accenture is looking for the best international talent: young graduates without previous professional experience as well as for experienced specialists, to join their team. ESN helps Accenture to reach out to young graduates and workers to bring to them different positions to which you can apply directly via Accenture's career site.
Erasmus Student Network is committed to improve the information flow between renown companies' job offers and international students to guarantee that every student has the same opportunities to access the labour market.


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Product Description
SIP Premium Engine Oil Seal Set
Oil Seal Set engine
for Vespa 125 GTR 3°/TS 2°/150
Sprint V 3°/200 Rally 2°/P80
CORTECO, 24x35x6/ 31x62,5x6/
27x47x6 mm, blue/blue/blue
incl. O-rings
Radial shaft oil seals should be installed firmly into the crankcase for a tight and secure fit. The inner circumference of the ring is the contact point to the rotating shaft and is mostly held in place with a circular spring to keep it sealed tight around the shaft. These items wear out regularly and should be replaced during every service of your motor. If the motor has not been run for a while the seals could be damaged. If the revs of your motor increase while it should be ticking over, the oil seal on the flywheel side of the crankshaft is probably defective or damaged. If you discover fuel mixed with your gearbox oil, the oil seal that sits on the clutch side of the crankshaft is the likely suspect.
We have oil seals produced by several manufacturers available, constructed using a variety of materials. The blue and black versions are made of a synthetic rubber, the blue type mostly produced by CORTECO are a little tougher than the black type which can be found in most engines as an OE PIAGGIO component. The brown seals are produced using a fluoro polymer material (FPM) are especially heat resistant (up to 160°) are harder wearing with more resistance against oil. Both types see wide spread use in all two -stroke motors.
Here at SIP there is no consensus amongst us as to which type is best, so we have solved this problem as PIAGGIO has done and stock all colours and types to keep everybody happy.
Helpful hint: As a rule of thumb, to ensure correct positioning of your fresh oil seals, the 'open' side of the seal always faces towards the one of the chambers that it separates, with the higher pressure. This higher pressure also helps the inner circumference maintain the seal to the shaft by pushing the inner edge against it.
TECH-TIP: Loctite oil seal adhesive keeps oil seals in place effectively, even in worn crankcases.
We have engine shaft oil seals available from PIAGGIO, PASCOLI and RMS. We here at SIP also compile some of the kits. We all rely on the supply of oil seals produced by either CORTECO, ROLF or other major engine oil seal manufacturers in order to put these kits together. Later PIAGGIO produced engines are provided with clutch side crankshaft oil sealing spring that are made of metal. The PASCOLI versions have an 'o' ring instead. This ensures that we have more than one set of oil seals available for every engine that belongs in a Vespa, which helps ease supply bottlenecks that can arise when dependant on an individual manufacturer. The Vehicle Identification Numbers (VIN) provide by PIAGGIO, PIAGGIO Vintage and PASCOLI do not always correspond with that of the specified oil seal necessary for certain earlier models of Vespa so check and compare the exact dimensions of the existing oil seals correspond with the ones to be ordered.
The rounded or squared recesses displayed in the design of the oil seals in earlier Vespa engines helps the cooling and lubrication of the adjacent bearings. The relative shape of the two designs is irrelevant as they are interchangeable.


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The demo for Dante's Inferno is coming to the Xbox 360 and PlayStation 3 this month, but one console is getting it two weeks ahead of the other. Who gets it first? Hint - it's not the Xbox 360.
I suppose that was a rather large hint, but I just couldn't keep our readers in suspense. The Dante's Inferno Gates of Hell demo will be hitting the PlayStation Network on December 10th, arriving on Xbox Live two weeks later on the 24th. Announced last month, the demo features the entire opening level of the game, detailing Dante's journey to Hell's gates in order to rescue his murdered love, Beatrice.
"The world of Alighieri's Dante's Inferno is such a rich, detailed, and often alarming universe, that all of us at Visceral Games put our hearts, and dare I say souls, into creating something truly intense and exciting," said executive producer Jonathan Knight. "We hope gamers enjoy this tease of hell, and come away from the experience lusting for more when the game comes out on February 9."
In the spirit of the holidays, I urge PlayStation 3 owners to invite an Xbox 360 owner to their home on December 10th in order to promote console harmony. Call it a kinder, gentler way of saying "go to hell."


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5 years of YPARD: a life-changing journey
"Being a YPARD representative gave me so many empowering opportunities, but also a chance to empower others" Testimonial by Ivana Radic Jean highlighting her experience and journey in working with youth in agriculture within the YPARD Serbia community since February 2013.
YPARDian bags home the 2017 SFIAR Master Thesis award!
In 2017, YPARD member, Sebastian Mengel, was the recipient of the Master Thesis award for his collaborative research with YPARD, HAFL and CGIAR CRP drylands program on agricultural livelihoods of rural youth in the drylands of Midelt, Morocco.
Good-bye from YPARD Nepal: Creating a space for young leaders
This testimonial by Dinesh Panday highlights his experience in working with youth in agriculture within the YPARD Nepal community since 2012. He is currently a doctoral graduate research assistant (2016-2019) at the University of Nebraska-Lincoln and the former YPARD Nepal country representative.
A season of innovation
The climate-smart irrigation system was designed with the aim of addressing these challenges; by collecting rainwater, transferring it below the soil surface and using it during the time of low rainfall thus making water available and accessible for perennial plants all year round.
Climate resilient agriculture and producer-consumer justice through ICT
Sustainable Development Goal (SDG) 13 was created to take urgent action to combat climate change and its impacts to ensure climate resilient agriculture and ICT based business promotional interventions from producer to consumer level. Youth, therefore, can play a supporting role to increase awareness and promote the agenda of SDG for agricultural sustainability to make sure others are aware of it.
From nowhere to somewhere; YPARD made a way
Marc Ghislain tells about all the changing scenes in his life as a young professional in agriculture: the anxiety in choosing a profession in agriculture and how his engagement with YPARD helped him find his feet in the agricultural sector.
A non-traditional career path
I strongly support and believe in an inclusive and equitable quality education together with lifelong learning opportunities for all. It is very important that we start to facilitate more flexible career paths in science if we want to encourage diversity and creativity. Being a lifelong learner is a value of sustainable self-development. It opens the door to life-changing, rewarding, fulfilling and continuous growth.
Easier said than done: Gender equality and women empowerment
The role of young people in helping shift gender perceptions is paramount. We can no longer employ tradition and culture as excuses for allowing basic inequalities between genders to occur and to keep perpetuating themselves. We need to acknowledge and cherish culture, but also acknowledge that culture is not a static entity and that it can, and must, evolve towards a more equal society.
Believe in sustainability, food security, fight for the hungry: My Credo
In an era where population, technologies and modernization are fast growing, meeting up with the basic food needs of the population worldwide is one of the greatest challenges nations and international institutions are witnessing. Marc Ghislain shares his views on how the young people can be part of the solution and more importantly in the achievement of the Sustainable Development Goal number two.
La lutte contre la pauvreté : motivation, passion, profession et engagement!
Aimé Kazika describes his engagement with other young people to raise awareness so that they become more involved in the agricultural sector within the African and Congolese context.


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Timber Wolf (Mad Cat)
Sarna News
- This article is about the iconic OmniMech. For other uses, see Timber Wolf (disambiguation).
|Timber Wolf
|Production information
|WC Site 1, Wolf Clan Site #2, WC Site 3, WC Site 7[1]
|Production Year
|24,233,124 C-bills
|Technical specifications
|Mech type
|Clan OmniMech
|75 tons
|Type W3 Endo-Steel
|Composite A-2 Ferro-Fibrous
|Starfire 375 XL
|Communications System
|Khan Series (Type 2c)
|Targeting Tracking System
|Series III OPT
|Heat Sinks
|15 Double heat sinks
|86.4 km/h
Primary Configuration
The Timber Wolf is a fast, heavy OmniMech. A signature design of the Clans' military might, it was arguably the first Clan OmniMech encountered by Inner Sphere forces (as opposed to periphery forces) on The Rock in 3049 by Phelan Kell.[3][6][7]
Vaguely resembling a cross-over between the MAD (Marauder) and CAT (Catapult) series, the Timber Wolf was tagged with the Inner Sphere reporting name Mad Cat on first contact. (The targeting computer on Phelan Kell's Wolfhound switched between MAD and CAT when trying to identify it; upon analyzing the data recording from Kell's 'Mech, Precentor Martial Anastasius Focht later officially designated it "Mad Cat".[3][6][7]
The Timber Wolf is vastly more powerful than either the Marauder or the Catapult. It uses its speed and firepower to engage an enemy at the range of its choosing. When first encountered by Inner Sphere forces the idea that a heavy 'Mech could move so swiftly while being as heavily armed and armored as the Timber Wolf was inconceivable. With its twelve tons of Ferro-Fibrous armor and arsenal that rivaled that of most assault 'Mechs of the era, it was completely alien to Inner Sphere military planners, commanders, and even the Precentor Martial of ComStar. Its technological secrets have since been reverse-engineered by the Inner Sphere.[3][6][7]
First introduced in 2945, the Timber Wolf was designed by Clan Wolf as a second generation OmniMech along with the Gargoyle and the Naga to replace the aging Woodsman. While the Gargoyle may have been a very capable, fast assault OmniMech, and the Naga may have been an excellent fire-support OmniMech, Clan Wolf (and indeed every other Clan) instantly realized the value of the Timber Wolf; thus, production rights to the Timber Wolf were jealously (and successfully) defended. Only through trade, gifts and battlefield salvage has the Timber Wolf entered the armies of other Clans. Until the invasion of the Inner Sphere, production of the Timber Wolf has been limited to a single facility on Strana Mechty.[3][6][7]
Weapons and Equipment[edit]
The default configuration of the Timber Wolf features a well blended mix of energy and missile weaponry; primarily extended-range lasers and long range missile racks that give the Timber Wolf considerable firepower at medium and long range. A pair of LRM-20 racks supplemented by two Extended Range Large Lasers make up the bulk of the Timber Wolf's firepower. The Timber Wolf also has an array of lighter, shorter ranged weaponry for use once the 'Mech draws closer to its prey: two ER Medium Lasers and a Medium Pulse Laser. Finally, it has two Machine Guns for point defense against infantry. Even on the modern battlefield the primary configuration of the Timber Wolf is a force to be reckoned with.[3][6][7]
Alternate Configurations[edit]
- Alt. Config. A
- Configured specifically for long range, direct fire combat, the A configuration of the Timber Wolf is armed with a pair of ER PPCs. These give the Timber Wolf A the capability of stripping nearly two tons of armor off of an enemy 'Mech at extreme ranges. For close combat, the A configuration has three Medium Pulse Lasers, an ER Small Laser, and a Streak SRM-6 to find any weak points in an enemy's armor. BV (1.0) = 2,470[4], BV (2.0) = 2,854[3][8]
- Alt. Config. B
- Armed to engage an enemy at either short or long ranges, the B configuration is an attempt to use the Timber Wolf in a workhorse role. For long range combat, a Gauss Rifle and an LRM-10 launcher gives the Timber Wolf B a powerful long range punch. For short to medium range engagements, a Large Pulse Laser is carried. Finally, for short ranges, a Small Pulse Laser and SRM-4 launcher are carried. Additionally, both of the missile launchers are linked to an Artemis IV Fire Control System to allow for greater missile accuracy. BV (1.0) = 2,012[4], BV (2.0) = 2,224[3][9]
- Alt. Config. C
- A long range configuration of the Timber Wolf, the C configuration carries an assortment of long range weapons. For direct fire capabilities, the Timber Wolf C mounts two ER Large Lasers and an Ultra Autocannon/5. For indirect fire support capability, its two LRM-15 launchers provide an ample quantity of firepower. Finally, for close range protection, the C carries an ER Medium Laser and an Anti-Missile System. BV (1.0) = 2,164[4], BV (2.0) = 2,500[3][10]
- Alt. Config. D
- This configuration mounts a pair of ER PPCs as its primary weapons. These are supported by four Streak SRM-6 launchers with two in the left torso and two in the right torso. One Streak SRM-6 on both torsos is rear mounted. A single ER Small Laser rounds out the Timber Wolf D's arsenal. BV (1.0) = 2,351[4], BV (2.0) = 2,682[3][11]
- Alt. Config. E
- Configured to use the added flexibility of the new Advanced Tactical Missile system, the E configuration mounts two ER Large Lasers as its primary direct fire weapons. The E Configuration also carries two ATM 9 launchers which can fire ammunition that is tailored to the ranges which it is engaging the enemy at. Finally, it carries a Light TAG designator for calling in Arrow IV artillery fire. BV (1.0) = 2,194[4][12], BV (2.0) = 2,444[3][13]
- Alt. Config. F
- This variant takes the primary configuration, downgrades the pulse laser to an ER Medium and replaces the Machine Guns with three Anti-Personnel Gauss Rifles. BV (2.0) = 2,764[3][14]
- Alt. Config. H
- A configuration that is capable of engaging an enemy with devastating effect at any range, the H configuration carries a pair of LRM-20 launchers linked to an Artemis IV Fire Control System for long range firepower. For close combat, two Heavy Large Lasers are carried, each of which can strip a full ton of armor from an enemy 'Mech at short ranges. Finally, seemingly added almost as an afterthought, an ER Small Laser completes the H's weaponry loadout. BV (1.0) = 2,095[4][15], BV (2.0) = 2,627[3][16]
- Alt. Config. S
- A highly maneuverable urban combat configuration of the Timber Wolf, the S mounts five jump jets, allowing it to jump up to one hundred and fifty meters. To offset the inaccuracy from using jump jets, the S configuration is armed with a Large Pulse Laser backed up by two Medium Pulse Lasers. The S configuration also carries four SRM-6 launchers and two Machine Guns, making it a deadly close combat fighter. BV (1.0) = 2,229[4][17], BV (2.0) = 2,462[3][18]
- Alt. Config. U
- Similar to the primary configuration's armament, this version is used in underwater combat. In addition to a BattleMech HarJel System that protects the torsos from hull breaches, the Timber Wolf U also carries four UMUs that allow it to cruise through the water at 43km/h. The weapons are restricted to a pair of Artemis IV-enhanced LRT-15s on each shoulder, while each arm carries an ER Large Laser and ER Micro Laser. BV (2.0) = 2,627[19]
- Alt. Config. Z
- Used by The Society's MechWarriors, this version of the Timber Wolf carries twin iATM 9 missile launchers with four tons of ammunition. Each arm carries an ER Large Laser and an ER Medium Pulse Laser. To maximize its battlefield utility it also carries a Nova CEWS in the center torso.[20]
Custom Configurations[edit]
- Pryde
- A non-standard alternate configuration of the Timber Wolf, this configuration was famously used by Star Colonel Aidan Pryde. The primary long range weapons are a pair of ER Large Lasers which are backed up by a pair of LRM-20s for long range combat. For close combat, two ER Medium Lasers are carried as well as an ER Small Laser. The Pryde Configuration also mounts four jump jets making it highly maneuverable. BV (1.0) = 2,624 BV (2.0) = 2,900[21]
- Bounty Hunter
- The first custom configuration used by the infamous Bounty Hunter of the time of the initial Clan Invasion, it mounted a Large Pulse Laser and a Medium Pulse Laser in each arm. It also had a Medium Pulse Laser in both the left and right torso with a Clan Light TAG in the center torso. A Light Active Probe, ECM suite, and a Targeting Computer provided stealth and increased accuracy. BV (1.0) = 2,393 BV (2.0) = 2,829[22][23][24]
- Bounty Hunter 2
- The second custom configuration used by the Bounty Hunter at the start of Jihad mounted a Large Pulse Laser in the left arm and paired ER Medium Lasers in the right arm, supported by a trio of SRM-4s in the left torso and similar trio of Streak SRM-4s in the right. Rounded out by an Anti-Personnel Gauss Rifle in each side torso and a ER Small Laser in the right, the Bounty Hunter's second Mad Cat also mounted a Targeting Computer as well as a Coolant Pod and three Jump Jets to enhance its effectiveness. A single ton of reloads for each weapon type was carried. BV (2.0) = 2,799[25]
- A custom configuration used by the Blessed Order's revived First Division's Unending Faith III, the Mad Cat BLO carries a ER PPC in each arm supported by Streak SRM-6 rack in each side torso and a ER Small Laser in the center torso. Carrying two tons of Streak reloads, its combat effectiveness is enhanced by TAG in the right torso and a Light Active Probe and Inner Sphere Improved C3 Computer mounted in left torso. BV (2.0) = 2,611[26]
Apocryphal Configurations[edit]
These variants were introduced in various apocryphal sources, and thus far have not appeared in any canonical media.
From MechWarrior Online:
- TBR-WAR "Warrant"
- A customized variant of the Timber Wolf rumored to be piloted by Vlad Ward, the Warrant uses an LB 5-X Autocannon in its left arm, with one ton of ammo. The right arm uses an ER Medium Laser and ER Large Laser. Each side torso carried an LRM-20 with one ton of ammo. The left torso supported a Medium Pulse Laser, while the right torso carries an ER Small Laser . A single ER Medium Laser in the right torso. [27]
Notable Pilots[edit]
- Vlad Ward, eventual Khan of Clan Wolf, was piloting a Timber Wolf on The Rock in 3049. Later, Vlad would make the Timber Wolf his primary 'Mech of choice, and would pilot one in the Great Refusal.[28]
- Arguably the most famous Inner Sphere pilot of the chassis, the Bounty Hunter piloted a Mad Cat that he captured from Clan Smoke Jaguar during the Clan Invasion and FedCom Civil War era.[22]
- Star Colonel Aidan Pryde of Clan Jade Falcon piloted a Timber Wolf during the Battle of Tukayyid.[29]
- On Tharkad, Phelan Kell commanded the troops of his Wolves from the cockpit of a Timber Wolf. [30]
Related Designs[edit]
- Woodsman - The Timber Wolf was one of three designs intended to replace the Woodsman. The Woodsman is an early OmniMech (either first or early second generation). The other OmniMechs intended to replace the Woodsman, the Gargoyle and the Naga, are closer in appearance to the original design.[31][32][33][34]
- Gargoyle - The Gargoyle was designed as another upgrade over the Woodsman; however, the Gargoyle mounts less armor and has less pod space than the Timber Wolf.[31][32]
- Naga - The distantly related Naga fire-support OmniMech was another variation of the Woodsman. The Naga extends the torso laterally to accommodate the placement of Arrow IV artillery systems.[32]
- Linebacker - The Linebacker was designed as an eventual replacement to the Timber Wolf. The Linebacker boasts a single improvement over the venerable Timber Wolf, a higher walking and running speed, allowing the Linebacker to keep pace with lighter, faster OmniMechs. Otherwise, the Linebacker mounts less armor and ten tons less pod space.[35]
- Mad Cat Mk II - Designed by Clan Diamond Shark for trade and profits, the Mad Cat Mk II is a 90-ton assault BattleMech, with a hull based strongly on that of the Timber Wolf. The Mad Cat Mk II has been dealt to other home clans and Inner Sphere armies, hence the choice of the Inner Sphere designation, "Mad Cat".[36][37]
- Mad Cat III - Following the success of the Mad Cat Mk II , the 55-ton Mad Cat III was an attempt by the Diamond Sharks to trade on the reputation of the original design in the medium weight class.
- Mad Cat Mk IV (Savage Wolf) - The third Clan Sea Fox BattleMech evoking the weapons loadout and visage of the Timber Wolf, the 75-ton Savage Wolf is very similar to the original, emulating the "D" configuration.
- Mad Dog (Vulture) - Created by Clan Smoke Jaguar, the Mad Dog Omnimech was derived from both Clan Coyote's Lupus and Clan Wolf's Timber Wolf moulds. Although using the same moulds could be considered a compliment, the naming proves otherwise as it was intended to be an insult and slur to both the Omnimechs and Clan designers.
- Rakshasa - Designed by the NAIS for the Federated Commonwealth, the Rakshasa chassis and weapons were designed to "emulate" the primary configuration of the Timber Wolf. The lack of Clan weapons and construction materials, however, prevent an exact duplication of the original's abilities. [38][39]
- The Timber Wolf/Mad Cat is one of the most iconic non-unseen BattleMechs, featured on the cover of the BattleTech Compendium, Technical Readout: 3050, and MechWarrior 2, as well as on the 20th Anniversary Classic BattleTech logo and the MechWarrior series icon. Its distinctive silhouette is now also the iconic symbol for the Clan Invasion era.
- Anastasius Focht, the Precentor Martial of ComStar named the Timber Wolf the "Mad Cat" after he studied the battle footage of Phelan Kell's encounter with Clan Wolf, noting familiar aspects with the Marauder and the Catapult. He also pointed out that it would be impossible for ComStar to replicate the 'Mech when Myndo Waterly suggested that their armorers modify their Catapults to that configuration.[6]
- The Timber Wolf/Mad Cat as originally depicted in Technical Readout: 3050 differed slightly from that present in later editions such as Technical Readout: 3050 Revised; the differences are due to changes in the official rules on constructing BattleMechs. Under the original rules, elements of 'Mech construction such as the engine and internal structure could be rounded off to the quarter-ton; this was subsequently changed, with rounding limited to half-ton increments. The original Timber Wolf/Mad Cat mounted a 375 XL engine weighing 19.25 tons and had an Endo Steel internal structure weighing 3.75 tons. When the rule systems were revised, the weight of each component increased to 19.5 and 4 tons each respectively; to compensate for the increase in weight the available Pod Space was reduced to 27.5 tons. This necessitated the following changes to the depicted configurations:[40][41]
- Prime: A single ER Small Laser mounted in the left torso of the original Prime configuration was removed;
- A: The number of Double Heat Sinks mounted in the left arm was reduced from 3 to 2, although the number in the right arm remained unchanged;
- B: A single ER Small Laser mounted in the center torso was removed;
- C: A single ER Small Laser mounted in the center torso was removed;
- D: The amount of SRM ammunition carried in the left torso was reduced from two tons to one ton, and a single ER Small Laser was added to the center torso.
- In German products the unit's proper Clan name was perfectly translated to Waldwolf. The unit's proper Inner Sphere name was altered to Katamaran, which means Catamaran.
Original Timber Wolf from TRO:3050
Timber Wolf from TRO:3050 Upgrade
Iconic Timber Wolf from BattleTech Master Rules, Revised Edition cover
Timber Wolf in urban terrain from CityTech, Second Edition
Internal Schematics of Timber Wolf from MechWarrior, Third Edition
A pair of Timber Wolf's attacking from BT:25 Years
Timber Wolf Prime in motion from CCG
Timber Wolf of Aidan Pryde from CCG
Model of Timber Wolf from MW4: Mercenaries
Model of Timber Wolf from MWO
- ↑ Objectives: The Clans, p. 31
- ↑ MUL online date for the Timber Wolf (Mad Cat)
- ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 Technical Readout: 3050 Upgrade, pp. 136-137, "Mad Cat OmniMech Profile"
- ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 Combat Operations, p. 108
- ↑ Record Sheets: 3050 Upgrades Unabridged (Clan & Star League), p. 110
- ↑ 6.0 6.1 6.2 6.3 6.4 6.5 Technical Readout: 3050, pp. 34-35, "Mad Cat OmniMech Profile"
- ↑ 7.0 7.1 7.2 7.3 7.4 Technical Readout: 3050 Revised, pp. 32-33, "Mad Cat OmniMech Profile"
- ↑ Record Sheets: 3050 Upgrades Unabridged (Clan & Star League), p. 111
- ↑ Record Sheets: 3050 Upgrades Unabridged (Clan & Star League), p. 112
- ↑ Record Sheets: 3050 Upgrades Unabridged (Clan & Star League), p. 113
- ↑ Record Sheets: 3050 Upgrades Unabridged (Clan & Star League), p. 114
- ↑ Record Sheets: Upgrades, p. 127
- ↑ Record Sheets: 3050 Upgrades Unabridged (Clan & Star League), p. 115
- ↑ Record Sheets: 3050 Upgrades Unabridged (Clan & Star League), p. 116
- ↑ Record Sheets: 3060, p. 190
- ↑ Record Sheets: 3050 Upgrades Unabridged (Clan & Star League), p. 117
- ↑ Record Sheets: 3060, p. 191
- ↑ Record Sheets: 3050 Upgrades Unabridged (Clan & Star League), p. 118
- ↑ Record Sheets: 3085 Unabridged — Old is the New New, p. 147
- ↑ The Wars of Reaving Supplemental, p. 73
- ↑ Record Sheets: 3050 Upgrades Unabridged (Clan & Star League), p. 120
- ↑ 22.0 22.1 Interstellar Players, p. 140, Record Sheet for Mad Cat Bounty Hunter with year 3060.
- ↑ Record Sheets: 3050 Upgrades Unabridged (Clan & Star League), p. 119
- ↑ BattleTech Dossiers: The Bounty Hunter, p. 30 "Mad Cat Bounty Hunter 2"
- ↑ BattleTech Dossiers: The Bounty Hunter, p. 30 "Mad Cat Bounty Hunter 2"
- ↑ Spotlight On: Unending Faith, p. 18 "Mad Cat BLO"
- ↑ MechWarrior Online - "Clan Heroes Sale Page"
- ↑ Malicious Intent, pp.13-15
- ↑ Falcon Guard
- ↑ Endgame, p.??[citation needed]
- ↑ 31.0 31.1 Technical Readout: 3050 Upgrade, pp. 136-138
- ↑ 32.0 32.1 32.2 Technical Readout: 3055, p. 140
- ↑ Technical Readout: 3055 Upgrade, p. 92
- ↑ Era Digest: Golden Century, pp. 25-26
- ↑ Technical Readout: 3055, p. 138
- ↑ Technical Readout: 3067, p. 132
- ↑ Technical Readout: 3050 Upgrade, p. 136
- ↑ Technical Readout: 3055, p. 72
- ↑ Technical Readout: 3055 Upgrade, p. 66
- ↑ Technical Readout: 3050, p. 34-35, "Mad Cat"
- ↑ Technical Readout: 3050 Revised, p. 32-33, "Mad Cat"
- BattleTech Dossiers: The Bounty Hunter
- Combat Operations
- Endgame (Novel)
- Interstellar Players
- Record Sheets: 3050 Upgrades Unabridged (Clan & Star League)
- Record Sheets: 3060
- Record Sheets: 3085 Unabridged — Old is the New New
- Record Sheets: Upgrades
- Spotlight On: Unending Faith
- Technical Readout: 3050
- Technical Readout: 3050 Revised
- Technical Readout: 3050 Upgrade
- Technical Readout: 3055
- Technical Readout: 3055 Upgrade
- Technical Readout: 3067
- The Wars of Reaving Supplemental
- Malicious Intent (Novel)
- Falcon Guard (Novel)
- Objectives: The Clans


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Differences between types of Titebond glue10/13
Sometime ago I recall reading a dissertation here about the differences between various types of titebond glue. As I recall it had something to do with hardness of glue line and glue lines telegraphing through finish.
We currently use both titebond 1 and titebond 2 in our shop though I am not really sure why. The titebond 2 has a pretty intense yellow dye that seemed to stain white plastic laminate so when we do this part we stick with the titebond 1.
If anybody can explain the various differences again I would appreciate it.
Franklin International has a pretty informative website with technical information on all their products as well as techs available by phone. I have been told verbally by a tech that Titebond 1 Extend has the most rigid glueline (read creep resistance) of all the Titebond products.
For what it's worth.
I'm a former Franklin employee and worked in the technical group until March of this year. Bill, your name sounds very familiar and I'm almost positive I've spoken with you at some point. If you're who I'm thinking of you requested an informational CD after IWF two years ago after talking with Dale.
Anyways, since departing Franklin I've founded a business that is focused on guitars (after consulting that industry for a bit over a decade.) I have no industry affiliations with respect to adhesives: So here's the straight dope on the differences:
White glues were the first PVA based adhesives and had little else to them. As a result they had lousy water resistance, were highly prone to creep, and generally wound up with a poor reputation compared to hot and liquid hide glues that were dominant in the woodworking industries at the time (mid 40s.) In order to improve their performance across the board (tack, creep resistance, thermal resistance, water resistance, to name a few) tackifying resins were added. In case you're wondering, this is all that "aliphatic resin" glues consist of. I often call them resin-modified PVA's because I consider it a less confusing term. This modification would not change the color on its own, so the decision was made to add yellow dye to differentiate them from white glues and is purely a marketing strategy. I have personally used dye free Titebond II and tested low quality yellow glues from manufacturers who have done nothing more than add dye to white glue. Uninformed individuals may try to convince you that yellow was added because it provided a better match to the surrounding wood. This is simply untrue. Not to mention, if you can see a glue line it's too thick. As part of some competitive testing I performed on Gorilla Wood Glue (not the polyurethane... The type II water resistant white glue) I found that not only was it nearly colorless and clear when dried but its performance was effectively identical to that of Titebond II. If you need something colorless for an exterior application I would not hesitate to recommend Gorilla Wood Glue. Titebond III is a different animal. It was actually the end result of a project to achieve type I water resistance from a PVA as a method to compete with urea-formaldehyde adhesives. It does accomplish it, but unless you're building a cutting board, countertop, or skateboard, I wouldn't recommend it. The product is prone to creep, and there's a reason its shear strength at 150°F isn't published: it's not great, usually around 1000 PSI which equates to a 2/3 loss of bond strength.
In terms of strength, at room temperature a good white glue can cause 100% wood failure just like Titebond Original, Extend, Titebond II, and Titebond III. Under ASTM D-905, they are also tested for shear strength at 150°F at which point from best to worst the list will look as follows: Titebond Extend, Titebond II Extend, Titebond Original, Titebond II, Titebond III. All PVA based adhesives are thermoplastics, which simply means they will melt (most of them reach their melting point somewhere above 250°F.) This is known as thermal plasticity, and in case you're wondering, it correlates exactly with creep resistance. It's also the reason that as a guitar manufacturer I personally use primarily Titebond Extend and Titebond 50. They're overkill, but if a guitar gets left in a hot car I can still feel confident that it wont come apart.
I've seen the term crosslinking a lot in this thread and some confusion about how it relates to creep. Picture plain PVA adhesive as wet spaghetti noodles: If you leave them to dry they will tangle around each other and shrink a bit, and you won't be able to pull the mass apart easily. Put that mess back into water and they will separate. Crosslinking PVAs would look more like spaghetti noodles that actually fused together as they dried and if you put them into water again those fused points (just like covalent crosslinks) would not separate. However, they do *not* crosslink with the wood itself. Instead, they have relatively weak electrostatic interactions (known as hydrogen bonds and van der waals forces) but in HUGE numbers, which is why they still create bonds stronger than wood.
I saw Bob's name mentioned, you're welcome to contact him if you wish. I helped train him, and unlike myself he comes from the sales/marketing world and still has an obvious corporate affiliation. In my case: I'm an entrepreneur with no specific allegiance to any adhesives company aside from my father who works for their industrial division. My Dad is the reason that despite being a younger guy I have been learning about the adhesives industry for over 25 years. I do my best to keep an eye on message boards because even though it's not technically my responsibility anymore, I care about getting good information out to fellow woodworkers. Not to mention, at over 5 months unemployed and just starting to get endorsements and contract work... It makes me feel like I'm still contributing something to the world.
I'm sure I've opened a can of worms, so feel free to reply or contact me directly. I'm always happy to help.
Thanks Kevin & Richard.
That was a fascinating read. Thanks for posting it.
Hugh mentioned the concept of "creep". I would like to make sure I understand this.
Several years ago we used to use a glue called Dorhus Express. It was a very thick white glue. We used it because it set up very quick. You didn't have to leave things in clamps very long at all.
The problem with this glue is you would clamp a piece of lumber that was perfectly aligned to a face of plywood. When you came back a few minutes later it would have slid a little bit and adjusted position.
Sometimes after we glue three sticks of lumber together and run it through the widebelt sander you can see a pronounced glue edge. The glue forms a little ridge and is slightly taller than the adjacent lumber.
What is this called & what causes it?
"and run it through the widebelt sander you can see a pronounced glue edge" Reasons: surfaces not perfectly prepared resulting in thick glue where they didn't meet tightly. Not long enough time elapsed for the glue to fully set hard. Not enough clamp pressure. Water absorbed from the glue is swelling the wood @ the joint, wait longer to machine.
I like gluing right off the straight line saw. I think the edge is more open and allows better bonding than joints that have been jointed.
Ca maker...the ridge is caused by the heat in sanding causing the glue to soften and then the pressure on the joint (after sanding) pushes a little of the adhesive out. Using fresh sandpaper, which has much less heating and reducing the amount of material removed (do not use a sander to do what a jointer or planer should do) will reduce the heat. You can also use an adhesive that is less prone to softening when heated...there are many different formulations of the PVA adhesives that are available.
Hmmm... So none of my thoughts on glue problems on glued up panels was it. Good aim Gene. Thanks always appreciate your input.


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random Unpublished photos1. Asos cardigan and Celine bag2. Homemade chocolate milk 3. Spring on a plate; Mozzarella salade May 8, 2012 Share on Previous articlelazy sunday Next articlefoodporn You may also like Kaya – Coconut and Egg Jam – Nyonya recipe #1 Healthy Breakfast Ideas for Every Appetite and Schedule Changing eating habits avocado mousse and shrimps Christmas gift idea #4 chocolate and bonbons Oysters for Christmas Super quick Citrus cured salmon gravadlax Marinated Herring Salad Nutella Oreo milkshake 21 Comments Lucy says: May 8, 2012 at 11:44 am Ik vind jou foto's altijd zo prachtig!X Reply Esmay says: May 8, 2012 at 11:21 am Die chocolademelk <3 Reply simon71688 says: May 8, 2012 at 11:05 am This looks very stylish, very well designed, I think you will like Best Regardsbelstaff women jacketbelstaff men jacket Reply garde says: May 8, 2012 at 10:54 am love the first pic. your look is great!kissesfran showroomdegarde.blogspot.com Reply Myrthe says: May 8, 2012 at 3:54 pm Chocolademelk! Jammie! <3 Reply Femme Virtue says: May 9, 2012 at 10:41 am love ur cardigan! Reply TheMinx says: May 8, 2012 at 6:32 pm love everything here! that sweater looks so comfy, and the salad is gorgeous. Reply Confessions of A Confused City Girl says: May 9, 2012 at 9:51 am That homemade cocoa looks sooo yummy… as the weather gets warmer i have to cherish these last few moments w/ hot chocolate ~SherineCheck out my blog, comment, and follow if you like! Confessions Of A City Girl Reply Foodloveandhappiness says: May 11, 2012 at 4:34 pm Leuke foto´s En wat een prachtige salade! Reply Lisa Rosalie says: May 11, 2012 at 6:40 pm Mooie foto's, mooie tas en mooie salade Reply MRSKA says: May 8, 2012 at 4:03 pm Hmmm.. Reply Isa says: May 10, 2012 at 5:07 pm like like like!where can i buy the sweater what you wear on your first picturee?x Reply Jessica says: May 10, 2012 at 1:17 pm the salad look amazing Reply Buaket says: May 8, 2012 at 12:18 pm love your bag! Reply devorelebeaumonstre. says: May 9, 2012 at 8:33 pm great photos! p.s. I'm having a jewelry giveaway if you'd like to check it out. devorelebeaumonstre.com xx Reply IngeBambi says: May 8, 2012 at 11:20 am I like xoxo Reply Diana says: May 11, 2012 at 2:16 pm I'm loving the cardigan! Reply Carlijn says: May 8, 2012 at 7:42 pm Niiiice, and that salad looks very good! Reply Joana says: May 8, 2012 at 11:53 am Celine bag = love! - Joana Reply Lemiza says: May 8, 2012 at 12:30 pm Prachtige foto's en de Celine bag is supermooi! xo Reply Sabrina T. says: May 8, 2012 at 10:58 am love the cardigan dear!! Patchwork à Porter Reply Leave a Reply Cancel reply Your email address will not be published. Required fields are marked * Name * Email * Website Comment You may use these HTML tags and attributes:   <acronym title="">
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Craft is an occupation that requires special skill especially manual skill. Art and craft are almost interrelated things and often these two words are overlapped in the usage.
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J. K. CreationOpposite Jay Corner, Near Kheta Talav , Collage Road,
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This Site/Concept is for sale. For further inquiries please contact Karan Garg. Email: firstname.lastname@example.com. Contact No: +91-8750051913


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Named scholarships
When it comes to earning a college degree, the greatest obstacle can be funding.
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Scholarships truly are the best way to ensure that today's students persist to graduation. Scholarships allow students to enroll full time and remain focused on their studies; they also reduce drop-out rates, decrease the stress of student loans, and shorten the road to graduation.
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If you are interested in making a significant contribution to student success, please consider a gift to the MAKING A DIFFERENCE AND MOVING FORWARD scholarship campaign, which is the University's most important initiative. You may also establish a named scholarship at The University of Akron, which can be created to honor a living person, in memory of a loved one, or to contribute to the growth of an area of study.
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McCready, James P. Family Foundation Golf Scholarship
Established in 1988 by the James P. McCready Family Foundation, The James P. McCready Family Foundation Golf Scholarship provides tuition aide for undergraduate students who are members of The University of Akron golf team. A former Zip golfer, Mr. McCready desires to provide assistance for those who are earning a college degree while representing the University's intercollegiate golf program. The selection of scholarship recipients from funds generated annually from this endowment will be made by the head golf coach.


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Events for Week of December 20, 2010
This weekend was low key as 1/3 of Beer Blotter headed to Ohio and is currently enjoying all that the Mid-west has to offer- Bells Cherry Stout, Great Lakes Christmas Ale and so much more! But now for your week in beer.
Tuesday, December 21, 2010
Christmas Beer Night: Philadelphia, Pennsylvania
7:00PM-12:00AM @ London Grill Bar And Restaurant
Celebrate the upcoming holiday, check out this tap list!
On Tap for the night…
Anchor 2010 Christmas
Great Lakes Christmas
St. Bernardus Christmas
Sly Fox Christmas
and the always anticipated…Troegs Mad Elf.
Wednesday, December 22, 2010
Mother of All Tastings: Pacific City, Oregon
6:00PM-8:00PM @ Pelican Brewing Co.
With the release of 2010 Mother of All Storms, a barrel aged barleywine from Pelican Brewing Co., stop by and enjoy a vertical tasting of the beer from 2008, 2009, and 2010 Mother of All Storms (previously The Perfect Storm)!
Also pouring, 2005, '06, '07, '08, '09, and 2010 Stormwatchers Winterfest Barleywine!
Thursday, December 23, 2010
Ugly Sweater Party: Bellaire, Michigan
7:00PM-11:00PM @ Short's Brewing Co.
Come celebrate Short's reopening in style. So throw on your worst sweater and receive $1 off pints from 7 PM until close!
Friday, December 24, 2010
What's going on tonight?: Everywhere, USA
Find your favorite people and your favorite bar- go there, and toast to the loved ones around you.
Saturday, December 25, 2010
Sunday, December 26, 2010
Boxing Day Beer Festival: Philadelphia, Pennsylvania
11:00AM-11:00PM @ Memphis Taproom
The holiday isn't over in countries like Canada and England, so get up early and stop by the Memphis Taproom!
December 26th Memphis Taproom will be serving some
of the best Holiday & Seasonal Ales from around the world.
As in years past, in addition to celebrating great beer, we'll be celebrating the true spirit of Boxing Day by collecting canned food and gently used winter coats and warm blankets for donation to local organizations that distribute to families in need.
Earn $1 OFF EACH BEER you have by bringing
in a coat, a blanket, or 3 cans* of food!
(*The goal is to collect cans of food that is nourishing. Please, not just any old can of water chestnuts from the back of your cupboard!)
Christmas and Winter beers will rotate steadily
throughout the day and will include:
Burton Bridge Bitter (on the engine)
Scaldis Noel
DuPont Avec Les Bons Voeux
Corsendonk Christmas
Allagash Fluxus 2010
De Ranke Pere Noel
Hitachino Red Rice Ale
De Dolle Oerbier
Port Older Viscosity
Sly Fox Christmas
Not to mention the following casks, poured off the edge of the bar:
Hannsen's Oude Gueuze
Rare Franconian Lager
Dogfish Head 75 Minute IPA
If you go to an event, want to let us know about an event, we would be happy to post about it. Please leave a comment or email me at firstname.lastname@example.com!


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World champion Tadese faces tough challenge in Birmingham - PREVIEW
Provided by IAAF
Birmingham, UK - Zersenay Tadese will return to the city where he scored the fourth of his five IAAF World Half Marathon Championships victories when competing at the Bupa Great Birmingham Run on Sunday (21).
The Eritrean only a fortnight after notching up his latest global title with a runaway win in Kavarna, Bulgaria, will start favourite although he can expect Kenyan rivals Micah Kogo and Eliud Kipchoge to mount a stern test over the 13.1 miles distance.
The presence and ability of the African trio will almost certainly see a sub one hour performance achieved and the UK All-Comers' record of 59:35 which Tadese ran when winning the World title three years ago looks likely to be rewritten.
It also seems inevitable in this IAAF Bronze Label Road Race with 18,000 runners which will be televised live by Channel 5 (10hrs to midday GMT+1), that Haile Gebrselassie's 12-month-old course record of 1:01:29 will be significantly lowered.
Indeed given the intensive rivalry of the trio, the fastest ever performance on British soil on the slightly downhill Bupa Great North Run course of 58:56 which Martin Mathathi set last year could be seriously threatened.
"It is only two weeks after I ran in the World Championships and I am still a little bit tired," said Tadese, a former World Cross Country champion and Olympic Games 10,000 metres bronze medallist.
"I don't know what to expect but they are two very good runners. I will try my very best to break the records. But the weather will be very important."
Tadese who has eight times in his career reached under the milestone one hour marker holds the World record of 58:23 which he posted in Lisbon two years ago, while his 2012 fastest is 59:34 which he also ran in the Portuguese capital.
Now having defeated the world's best he arrived in Birmingham on Thursday night (19). Even after a lengthy delay at immigration there was a spring in his stride.
On what is expected to be misty Sunday morning with a temperature approaching 15 degrees, if reproducing the natural front running tactics which claimed him victory in Kavarna, he will be the man to beat.
However, Kogo like Tadese a past Olympic 10,000m bronze medallist and former World 10 kilometres record holder, showed with his display in last month's Bupa Great North Run he has successfully made the transition to the longer distance.
Kogo in one of the closest ever Great North Run finishes lost out by only a couple of strides to fellow Kenyan Wilson Kipsang but came away from the Tyneside venue with a very impressive personal best time of 59:07.
Kipchoge, one of the World's finest ever track distance runners including winning the 2003 World Championships 5000m gold medal in Paris, decided after failing to make the Kenyan team for the London Olympics to switch to a road running career.
It began with a highly encouraging third place in last month's Lille Half Marathon where he recorded on his debut over the distance a very respectable time of 59:25. He struggled at the World Championships when suffering in the heat and humidity finishing outside the medals, but is determined to bounce back.
Abera Kuma, fifth in last year's World Championships 5000m final, will make his half marathon debut, while Abdellatif Meftah the French record holder over the distance with a time of 60:46 leads the European challenge along with seasoned British international Tomas Abyu.
Japan's Yusei Nakao who achieved a top six finish at the 2008 IAAF World Half Marathon Championships and Kenya's reigning Commonwealth Marathon gold medallist John Kelai will also line up.
Former World Half Marathon champion Berhane Adere is the undisputed favourite for the women's title and holding a personal best of 1:07:32 which won her the 2003 Bupa Great North Run, is much faster than her rivals.
However, the highly experienced Portuguese star Sara Moreira, who has recorded 1:10:08, is a very determined performer and will give the Ethiopian a run for her money at a venue which saw Gemma Steel clock the course record of 1:12:20 in last year's race.
Commonwealth Marathon silver medallist Irene Mogake of Kenya will also be in action and has a PB of 1:11:13, while Hannah Walker, third in the recent Bristol half marathon, will be looking to lower her fastest ever time of 1:11:50.
Dave Martin for the IAAF


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Since 1885, Barry-Wehmiller has been a trusted partner to packaging companies around the world. Among our various BW Packaging Systems businesses, we offer a broad range of expertise - from manufacturing, packaging and materials handling to process optimization, plant design and implementation, and more.
So whether you're looking for a single piece of equipment or the design and installation of a fully integrated line, BW Packaging Systems will deliver a tailor-made solution to solve your unique packaging challenges.
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Welcome to the Rockbox Technical Forums!
i just installed rockbox on my new e280 and unfortunately have the same problem now, too... rockbox works, but booting into the original firmware fails with the strange display.as far as i see, i can't even uninstall the bootloader because the sansapatcher does not find it anymore... (sansa dies when attached to the usb with the same symptoms as described for 'simple' booting.)
Rockbox boot loader Version: *something really long* Sandisk Sansa e200 Partition 0: 0x0B 3814mb Partition 1: 0x84 20mb Loading original firmware... Trying firmware partition BL mi4 size: EC00 OF mi4 size: 52B400 CRC32: 578F184B Model id: e200 Binary Type: RBOF Calculated CRC32: 578F184B
I'm having the same problem (as in the video) with my e280...
UPDATEI installed the unofficial reverse bootloader (loads OF first, hold select to load RockBox), and it works great. I'd prefer that I could load RockBox first, but I think I can live with this.
sansapatcher just puts the bootloader in place and obviously it works since it boots and it can load rockbox.I'm leaning towards that some of the (newer) mi4 firmwares want more magic done by the bootloader, like the newly added stuff for the c200 OF.
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Hello everyone.
I'm looking for ability to turn off files minification. Right now JSBuilder compress all project files while building but I really don't want my files to be minified with JSMin as I'm using YUI Compressor. Does anyone knows how can I disable that complression/minification? I didn't find anything about that in optionsm ;(
Please, advice. Thanks!


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Guinea opposition mulls new protests
Guinea's opposition said they were considering new street protests following the expiry on Thursday of the 72-hour ultimatum they gave the government to fix the flaws they had identified in the roll of registered voters.
Opposition spokesman Aboubacar Sylla said the country's election commission had given them the electoral list on a thumb drive - but that is not what they sought. He said his coalition had demanded that the body publish the electoral list nationwide, so that voters could check if their names appear in their respective polling stations ahead of the Sept. 24 legislative election.
Sylla said the electronic list they were given bears more than 5 million names, making it virtually impossible for them to check its veracity.
"It's over 250,000 pages," he said of the electronic list. "How are we supposed to check this? It's not in Conakry at our headquarters that we can check if the names are right. It needs to be published in each locality so that people can go and see if their photo matches their name."
Moustapha Naite, campaign spokesman for the ruling party, said the opposition's claims were unfounded and amounted to a delay tactic.
"Today at 10 or 12 days before the legislative election, I find it very curious that they have found another pretext for delaying it," said Naite. "They have realized that the ruling party is making gains in the field. We are winning everywhere, including in their stronghold, and that is what has caused this panic (on their end)."
Guinea held its first democratic election in its 55-year history in 2010. Although the vote was deemed mostly transparent by international observers, the campaign that preceded it and the post-electoral violence that followed it, divided the country along ethnic lines, pitting the nation's two largest ethnic groups against each other. The legislative election, which was supposed to complete the West African nation's transition to constitutional rule, was due to take place a few months after the 2010 ballot but has been repeatedly pushed back.
The opposition - who are predominantly from the Peul ethnic group -have repeatedly taken to the streets. It's led to violent clashes with the mostly-Malinke security force, which supports the president, an ethnic Malinke who is accused of stacking the government with members of his ethnic group.
Callimachi contributed to this report from Dakar, Senegal.
Rukmini Callimachi can be reached at https://www.twitter.com/rcallimachi


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Featured Resource
The increased hurricane activity predicted for future decades has serious implications for the important work of Montgomery Botanical Center (MBG).
This Plan is intended to outline plans for preparing for emergencies and for immediate response and short-term recovery efforts in an emergency.
The history of the Morris Arboretum can be told through its eldest trees. Every scar and abnormality present on these immense specimens inspire awe, enrich visitor experience, and provide a glimpse into the past of the gardens.


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Tr:Duke Nukem 3D
Installing Duke Nukem 3D
Step 1: Buy the game from GoG.com and Download it. Or use your game cd. The install instructions are for the GoG version.
Step 2: Install with wine:
cd ~/Downloads ## path you downloaded to wine setup_duke3d.exe
have it install to your home directory like ~/dn3d
step 3: Now we get the SVN source for eduke32 and compile it.
If you don't have subversion yet: as root:
equo install subversion
Once you have that installed you can get and compile the eduke32 source code (not as root):
cd ~ mkdir src cd src svn co https://eduke32.svn.sourceforge.net/svnroot/eduke32 eduke32 cd eduke32/polymer/eduke32 make
Read the source documentation to make sure you have all the needed deps. If you have all the libraries needed like sdl and such, it will build 2 binaries, eduke32 and mapster32. Copy them to ~/dn3d and you can type ./eduke32 from the dn3d directory to play the game. You can edit the config files in ~/.eduke32 to get fullscreen support and such. If you have further questions, hop on irc: irc.freenode.net #eduke32
I find that if you don't have pkgconfig, it builds much easier.
For added graphics, enable polymer engine in the config file, and use the polymer version of the High Resolution Pack (HRP). These options do require a beefy video card with a good amount of memory.


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Jordan Bunker of Electromagnate wrote in to share some great news. ReMade, the hackerspace documentary that we posted about earlier, has successfully met their Kickstarter fundraising goal. Here's what they have to say:
Thank you so much for supporting our kickstarter. We are so deeply encouraged by the outpouring of support from the community. It's wonderful to know that so many people share our passion and desire to tell this story. It has really pushed our ambitions forward and has made us even more determined than ever to do it justice.
Currently, we have just under a week left and we have reached our initial goal. However, there's still some time left to support our project. Every little bit helps us improve our production and increases the number of locations we can feasibly cover so please spread the word! For your support we offer a few gifts, including a limited run t-shirt and poster!
Thanks again and keep making!
P.S. We are still sifting through the emails that we've received in the last couple weeks. Rest assured, we are reading every single one of them! So many to get through so please accept our apologies for not responding right away.
Congratulations, and we look forward to seeing the movie!


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What's New
- A great new update to our mobile betting system that gives you a smoother game and an updated user experience
- Some sweet new graphical updates and game tweaks
- Some minor bug fixes
App Description
Welcome to Slots War of China Town! Experience through varieties of Chinese themed level and WIN BIG!
-Spin to gain experience and unlock new levels
-Powerups: keep spinning to enable powerups that could reward you with anything from a fun bonus game to double winnings!
-Bonus games: Earn more coins with fun bonus games including card games and scratch cards!
-Memberships: achieve different member levels the more you play. Memberships reward you with new powerups, bonus games, higher bets and even exclusive new themes.
-Growing list of levels with fun sounds and animations.
-Game Center XP Leaderboard: See how you rank against other players.
-Play up to 25 lines for big payouts!
_ Free bonus coin payouts when you are not in the app
Stay tuned for updates with new levels and bonuses!
App Changes
- June 18, 2014 Initial release


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The Christian Bashers Defense team has pretty much taken over the comboxes on my recent post Constitutional Rights for Me, But Not for Thee.
They are as predictable as mosquitoes. Just say something really true about their behavior, and they show up, armed to buzz bomb you until you go inside and close the door.
In this instance, I asked the simple question: Do Constitutional rights apply to Christians the same as everybody else?
The answer should seem obvious. But of course it's not. The reason it's not is the bullies who want to limit other people's rights always get mad and deny what they are doing when someone calls them on it. They do it every single time.
We're all supposed to join them in their pretense that there's nothing discriminatory or offensive in their attempts to drive Christians from the public square. No one is supposed to challenge their idiotic pretense that using government controls to limit the free exercise of religion in this country is actually a push for freedom, instead of the tyranny that it is.
If we can't be agree with them, they want us to sit down and be quiet and stop contradicting them. If we don't, well then, they'll scream and shout until everybody gives up and lets them have the day.
It has always been thus. People who do things like this always deny it, and they always get mean when someone calls them on their facile denials.
That's why this particular post ended up being dive-bombed by a whole troupe of angry combox mosquitoes. Even though the readership of this blog is heavily — and I mean heavily — Christian, the Christian defenders were outnumbered. In fact, only three stalwart souls tried to stand up for Christ in these arguments. In the end, it got down mostly to one stubborn Christian, who is hanging in there to this very moment.
For all that, this lone fellow managed to push the whole mosquito assault into a slow unwinding of their lies until, one of them just came out with it.
And I quote:
No one is forcing anyone to do anything. And no one is driving anyone out. But if it does not believe it can conscientiously comply with the law, the Catholic Church can sell its hospitals, schools, universities and charity organizations. And the church and its members have the right to protest and encourage that the law be changed.
Of course, that would dramatically change the face of the church in the United States.
And then the commenter goes on, blah, blah, blahing with a lot of stats (which may or may not be accurate. I haven't checked.) about the Church's holdings.
How about that? Not, mind you, that forcing the largest denomination in the country to "sell its hospitals, school, universities and charity organizations" if it won't violate its 2,000 year-old religious teachings due to government demands that it do so might be … ummm …. a slight violation of the principles of that fictional "wall of separation between church and state" of which militant atheist are so proud. Also, not that it might be an outright dismissal and abrogation of religious freedom as defined in the Bill of Rights. Nor that it might be just a wee bit of outright tyranny.
It's just deserts for those who have the temerity to think that their individual rights as free Americans amount to a hill of beans to the post Christian, militant secularist demands for moral conformity (with moral being defined by them and them alone) that must govern us all.
I want to remind you that this is about birth control and abortifacients. Nowhere that I know of is there a Constitutional right to free birth control and free abortifacients. Also, nowhere that I know of is there a Constitutional right to force other people to pay for your birth control and abortifacients, even, or in this case, especially if it violates their religious beliefs to do so.
There is, however, a pretty strong Constitutional right to the free exercise of religion. Not even President Obama is debating that. What he's trying to do is re-define this Constitutional Right to the free exercise of religion along the lines of how it is defined in Communist dictatorships. He wants to say that freedom of religion is actually just freedom of worship and that only in governmentally prescribed "houses of worship." And, oh yes, behind the closed doors of your own house.
It takes a combox firebrand to just come out and say what all this truly means and where it leads. It is leading to stripping the Church of all its "hospitals, schools, universities, and charity organizations" in what would certainly amount to a fire sale. It means driving the Church out of public life, totally and absolutely.
There's nothing dishonest about what this person said. In fact what's powerful about it is that it is the truth of where we are heading. It is exactly where we are going if this tyrannical abuse of the freedoms of Christians as citizens of the United States is allowed to continue.
If the Obama administration succeeds in redefining religious freedom in these terms, it will have destroyed the First Freedom of the American people.
And all this so that it could bend this country over and bow it down to the little g gods of abortion and death.
I want to thank the strong-hearted Christians who have hung in there during this debate. I encourage some of the rest of you to get in the game along with them. Standing up for Jesus is not a spectator sport. We all need to do it.


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Judge set to preside over 2nd phase of BP trial
BP's 86-day struggle to stop the flow of oil gushing from its blown-out well in the Gulf of Mexico will be the focus of a trial scheduled to resume Monday in federal court.
BP insists it was prepared to respond to the deadly disaster, but plaintiffs' attorneys will argue that the company could have capped the well much sooner if it hadn't ignored decades of warnings about the risks of a deep-water blowout.
U.S. District Judge Carl Barbier has set aside 16 days for the second phase of the trial, which will also feature dueling expert testimony about the amount of oil that spilled into the Gulf.
The trial's first phase ended in April after the judge heard eight weeks of testimony about the causes of the blowout.
Previous stories:
Study: BP spill did wide damage to sea-floor life
BP renews bid to suspend settlement payments
Halliburton manager charged with destroying evidence after BP's Gulf of Mexico oil spill
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The following certificate programs are available through the Division of Lifelong Learning. These programs are offered by and are under the academic control of the appropriate faculties and schools.
Students who wish to complete certificate programs must be admitted to the University as undergraduate students and comply with the General Academic Regulations (Undergraduate).


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BBC TV's Story of Ireland series reached 1798 last week and devoted 13 of its 59 minutes to the United Irishmen and the Irish Rebellion (or Revolution, as presenter Fergal Keane refers to it).
To be fair, the programme covered two centuries, from the aftermath of the Flight of the Earls to the defeat of the Rebellion and the death of Wolfe Tone.
The place of Ireland in the religious wars of the 17th Century, the plantation of Ulster under James VI and I, the arrival of the Scottish Presbyterians, the other Irish Rebellion – of 1641, the depredations of Cromwell and his successors during the Interregnum, the Williamite-Jacobite war, the Irish Ascendancy, emigration to America and Grattan's Parliament as well. A lot to squeeze into an hour.
And the scene was set well for the story of 1798.
So – what was there to praise about the programme?
It showed the United Irishmen and the 1798 Rising in good historical context – not just the European religious wars, but the revolutionary and republican movements which had seen American independence and the Revolution in France.
Rightly it pointed out that the early high-minded political and revolutionary movement of the (originally largely Protestant) United Irishmen changed as sectarian conflict and terrible military atrocities on a largely defenceless population swept more and more people into conflict.
It leaned quite heavily on Wolfe Tone, though of course he was vital to the story. And it was balanced – although there were multiple atrocities perpetuated by the military, there were also two by the rebels against the loyalists/government supporters, in Wexford and Scullabogue.
What was missing?
But it's puzzling that little or no mention was made of the yeomen, volunteer regiments which were responsible for many of those atrocities. Since Nicholas Delaney's story involves yeomen, I may come back to them in another post.
And there was no mention of the Battle of Vinegar Hill, where the rebel/United Irish army was finally crushed in the south-east, and the last pitched battle there – apart from what was more of a skirmish at Ballyellis, Nicholas's own home.
A story often told
Ever since the last man died and the last woman was raped at Vinegar Hill the story of 1798 has been retold and reinterpreted time and again – republican revolution, nationalist rising, agrarian rising, Catholic rising, early attempt at socialism, anti-English war, anti-Catholic war and much more.
I'm glad that it has now has been told in prime-time on a main BBC TV channel, even if the programme was too rushed for me and made those two surprising omissions.
For more background on the United Irishmen and 1798, try these links from Wikipedia and the BBC. There's so much on the internet, these seem good places to start. Can anyone suggest other good overviews?
New: Here's a link to the episode. What do you think?


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A discussion series focusing on arts and education, "Next Stage" is aimed at early-to mid-career artists in the fields of theatre, dance, music and visual arts. "Next Stage" will continue on a yearly basis, featuring artists moderating and speaking at the events.
Events of the Next Stage discussion are hosted and presented by Lincoln Center Education, in partnership with other New York City arts organizations.
Virginia Johnson, artistic director of Dance Theatre of Harlem, moderates the panel, which includes Abdul Latif, choreographer and artistic director, D Squared Dance Theater and former Lincoln Center Education Artist mentoring lab fellow; Camille A. Brown, choreographer and artistic director, Camille A. Brown and Dancers; and Seán Curran, choreographer and artistic director, Seán Curran Company. Dance/NYC, with executive director Lane Harwell, is partner.
The series' upcoming events follow:
May 30: Music
Moderator: Midori, violinist
Partner: New York Philharmonic (Theodore Wiprud, Vice President, Education) June 10: Visual Arts
Moderator: Andrew Goldstein, editor-in-chief of Artspace
All events in the series take place at 6 PM in the Clark Studio Theater, located on the seventh floor of the Samuel B. and David Rose Building at Lincoln Center, 65th Street and Amsterdam Avenue.
Tickets for Lincoln Center Education's "Next Stage" series are $10 and available for purchase at lincolncenter.org/nextstage, (212) 721-6500, and at the Avery Fisher Hall Box Office, Broadway and 65th Street.


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NOTE: When communicating online, your Marine's privacy is as important as Operational Security. Please respect YOUR Marine's privacy!
Friday: 3/6
Read an awesome news article about the 3/5 Marines written by a journalist who was embedded with the platoon.
You'll also find a heartwarming story about a Marine grandma who is sending her love to our troops through healthy meal kits she assembled.
Keep Voting For Wounded Warriors to Win
We are just below the winner's bracket at #17. We are voting like crazy with power votes and we hope you are too! There's only four days left until voting is cut off, and we need to move up at least two spots to win a grant!
Since we're nearing the end, moving up in rank is becoming more difficult. It's going to take A LOT of votes to bring us up at least to #15. So vote everyday, and spread the word! TOGETHER we can win this grant for our wounded warriors!
Click here to vote.
Visit www.VotePurple.com for more information.
Hearty Meals for Deployed Marines
Marine Grandma Sends Us "Meal Kits"
A few weeks ago we received 12 meal kits (see photo to right) in the mail from the grandma of a 3/5 Marine. These kits were packaged with care and contained healthy meals the Marines much prefer to the MREs they are given to eat.
Each kit also had a personal letter attached to the bottom of its box. In the letter the Marine grandma includes funny anecdotes about her small town. She also states that her son was with the 3/5 Marines and she constantly sent him packages. When 3/5 returned home, she wanted to continue sending packages to the platoon that was replacing 3/5, the 1/5 Marines. Though the meals were designated for 1/5, the platoon is heading home soon, so the meals will be sent to another platoon wanting supplies.
In the letter she also says, "You need to be reminded of the good wishes and prayers of the people of your country." The letter ends with, "God bless you and watch over you in your walk abouts and help you to sleep well at night. We on the homefront thank you for your service. Love, Grandma Stroh"
The Marines who receive these packages will be filled with encouraging words and good food. We thank Grandma Stroh for sending us the kits to forward on to our brave service men and women!
Dollar Campaign Success
Thanks to all of your generous donations, we raised a total of $8,972 for our summer Dollar Campaign. We didn't quite reach our goal of $12,500, but our cost of shipping over 1,000 care packages on Saturday, July 23rd was significantly lowered.
On behalf of the Marines who look forward to receiving these packages while overseas, we thank you again for your support of the Dollar Campaign! If you didn't get a chance to donate to this fund, it's not too late....you can still donate online by clicking here.
Stay tuned for a recap and photos of our July 23 pack day!
Red Friday Photos
EGA Store Supporter Spotlight
Marine Mom Maggie from New London, Minnesota, sent us this photo after her Marine's graduation from boot camp in June. She says:
"Here's our 'Red Friday Tribute'. Our Marine graduated from MCRD San Diego. This is Mom, Sis, Marine Dan, Sis-in-law, & big Bro. We wear our T-shirts proudly!!"
Thanks Maggie! We love to get photos from our supporters. If you have a photo you'd like to share, send it to firstname.lastname@example.com. To see other photos we've received visit our "Semper Family" photo gallery here.
Attention on Deck: From & About Our Marines
An April article on the Wall Street Journal's website featured the Marines of 3/5 and some of their experiences while deployed. The article was written by a journalist embedded with the platoon and gives a first-hand look into the daily lives of these Marines.
A poignant excerpt from the story reflects the bravery and tenacity of all Marines:
"We got a radio intercept yesterday," Lt. Garcia said. "Some Talib leaders in Pakistan were chewing out the local fighters for quitting. The locals yelled back, 'Marines run toward our bullets.'"
One of the Marines in the above photo (far left) stepped on an IED during 3/5's tour and was taken to San Diego's Wounded Warrior Barracks. He had to have his leg amputated, and he is now being cared for by our Purple Heart Hero Support™ volunteers.
Click here to read the WSJ article.
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We hope this week's edition of our email newsletter has been useful for you and your family. If there are topics you would like us to address, or if you have other suggestions for the newsletter, please contact us.


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Translation is the third stage of protein synthesis and proceeds in three phases: initiation, elongation and termination. This process involves "reading" a mRNA's codons to create a polypeptide chain.
In prokaryotes, initiation occurs when an amino acid is covalently bonded to a tRNA. [1] Next the ribosome binds to the 5' end of mRNA. This allows translation to build a particular protein. This process is halted once a stop codon (UAA, UAG, or UGA) is reached because no tRNA can bind to these codons. What does recognize this codon is a "release factor" protein that disassembles the ribosome/mRNA complex.
An illustration of this process is shown:
Similar to prokaryotes, eukaryotic translation has three phases. However, two types of initiation exist. Usually to initiate translation, proteins must interact with the 5'-end of the mRNA called the 5' cap. This cap is an altered nucleotide that consists of a guanine linked to the mRNA using a triphosphate link. This type of initiation is cap-dependent. The other type is cap-independent. This method of initiation is usually mediated by the Internal Ribosome Entry Site (IRES) approach. Elongation and termination proceed in a similar manner to prokaryotes.
Internal Ribosome Entry Site (IRES)
IRES is a nucleotide sequence found in the middle of a mRNA that allows for the initiation of translation without needing to start at the 5' cap. This task is accomplished by the ribosome being "guided" to the IRES by IRES trans-acting factors (ITAFs). During recent years, examples of this type of initiation has been shown to be used in processes like apoptosis where a specific area of the mRNA needs to be expressed to induce cell death. [2]
Bacterial Ribosome Binding Sites
A ribosome binding site (RBS) is a region 6-7 nucleotides upstream of the start codon AUG in prokaryotes called the Shine-Dalgarno sequence (5′–GGAGGU–3′). The ribosome will base pair with this site through its own rRNA as well at the start codon using tRNA. What makes this an interesting topic of research is the fact that the Shine-Dalgarno sequence is not the "optimal" RBS for all expression processes. In other words, a RBS affects the rate at which a particular Open Reading Frame (ORF) is translated. There are two general ways in which this happens: i) the rate at which ribosomes are recruited to the mRNA and initiate translation is dependent on the sequence of the RBS and ii) the RBS can also affect the stability of the mRNA, thereby affecting the number of proteins made over the lifetime of the mRNA.
RBS Sequence Design
The first variable to be taken into account should be the sequence of the RBS itself, specifically to what degree the RBS is complementary to the free end of the 16s rRNA. If this hybridization is highly favorable, then the time needed to complete the initiation phase will be reduced allowing elongation to proceed. Obviously the start codon AUG must be upstream of the RBS and needs to lie 6-7 nt. The reason for such a specific distance is due to the geometry of the ribosome and where it attaches itself to the mRNA. Not only is the exact sequence of the RBS important, but the overall sequence of the mRNA will affect translation rates. The iGEM registry has a nice collection of RBSs. iGEM RBS catalog Secondary structure in mRNA has been shown to affect translation rates [3] due to competitive binding of the RBS by the ribosome as well as other nucleotides in the mRNA. Many attempts have been made to computationally predict the best sequence to minimize secondary structure in DNA/RNA. CircDesigNA is one such tool .
Codon Optimization
Genetic code is defined by a sequence of three nucleotides, called codons. These codons can serve three functions. 1) The start codon, AUG is used to initiate translation 2) Specific
codons stop translation by not coding for anything 3) They serve as a template to allow the binding of amino acids to form a protein. Crick and Brenner et al. were the first to show that codons existed and that they coded for specific amino acids. [4] They translated a poly-uracil (UUUUUUU...) sequence in vitro and discovered that the only amino acid synthesized was phenlyalanine. Using similar methods, all codon combinations (4^3 = 64 total) were assigned to the twenty amino acids. This has led to developments in determining both theoretically and experimentally what codons work best for particular genes that need to be expressed[5]. This attempt at randomly varying the codons used the express GFP and found that there was a 250 fold range in protein levels across the 154 genes that were synthetically produced. However upon further investigation, they determined that secondary structure in the mRNA accounted for more than half of the variations in expression. They did not find that codon bias affected the results as much as anticipated. A review paper discussing different redesign strategies including modification of initiation regions, alteration of mRNA structure, and use of different codon biases gives a more thorough look at these topics. [6]
Eukaryotes vs. Prokaryotes
The heart of codon optimization lies in the fact that bacteria do not always use the same codon for amino acids that eukaryotic cells do. This has given rise to online tools that help determine the correct sequence needed to express genes in various host organisms. E coli codon usage
Alternative Genetic Codes and Orthogonal Ribosomes
There has been a push to create synthetic genetic code that can utilize unnatural amino acids in translation. However because the universal genetic code contains 61 codons that all code for a particular amino acid, designing artificial amino acids becomes quite difficult. One attempt to get around this is to create an entirely new genetic code that uses a mutated ribosome to allow for expression of more than 200 unnatural amino acids. [7] This genetic code is based on a four nt. codon as opposed to the traditional 3 nt. codon. To create a ribosome that can code quadruplet codons, they synthetically evolved an orthogonal ribosome and paired it with mutually orthogonal aminoacyl-tRNA synthetase–tRNA pairs.
Protein Expression with Rare Codons
As mentioned previously, sometimes proteins are not expressed well in other host organisms such as E. coli. This is compounded if the particular codons that have a low quantity of tRNAs compared to others are in abundance in the gene of interest. That is, there are more rare codons than usual in the gene of interest. To improve this difficulty, attempts have been made to adjust the codons that make up the protein making it more readily expressed in other organisms. An alternative to this approach is to alter the organism instead. [8] [9] One particular attempt tries to address low expression levels of recombinant protein from parasitic organisms. To overcome this, they transformed a plasmid that coded for three tRNAs (Arg, Ile, Gly) which will recognize the rare codons in their expression plasmid to enhance the expression levels. Previously, Brinkmann et. al. used a cotransfection method to help induce production of proteins by tranforming the tRNA Arg along with the gene of interest.
- Laursen2005 pmid=15755955
//Initiation of protein synthesis in bacteria.
- Gold1990 pmid=2199797
//Overview of high-level translation initiation.
- Crick1961 pmid=13882203
//General nature of the genetic code for proteins.
- Kudla2009 pmid=19359587
//Coding-Sequence Determinants of Gene Expression in Escherichia coli.
- Lopez-Lastra2005 pmid=16238092
//Study on the growing biological relevance of cap-independent translation initiation.
- Neumann2010 pmid=20154731
//Encoding unnatural amino acids using a quadruplet-decoding ribosome.
- Brinkmann1989 pmid=2515992
//Expression levels of recombinant genes in E. coli depends on the availability of dnaY gene product.
- Baca2000 pmid=10704592
//A method for high-level overexpression of Plasmodium and other AT-rich parasite genes in E. coli.
- Gustafsson2004 pmid=15245907
//Codon bias and heterologous protein expression


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See what questions
a doctor would ask.
Anodontia is a genetic syndrome that exhibits autosomal recessive inheritance in certain families.
Inheritance of Anodontia refers to whether the condition is inherited from your parents or "runs" in families. The level of inheritance of a condition depends on how important genetics are to the disease. Strongly genetic diseases are usually inherited, partially genetic diseases are sometimes inherited, and non-genetic diseases are not inherited. For general information, see Introduction to Genetics.
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Copyright © 2014 Health Grades Inc. All rights reserved. Last Update: 13 August, 2015 (2:43)


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SEPTEMBER WAREHOUSE RESTAURANT EQUIPMENT Online Auction
Inspection Date: Sunday, September 27, 2020, between noon-4pm at 6110 Edgewater Dr. Suite A, Orlando, Florida 32810 Auction End date: Sunday, September 27, 2020 at 8:00pm EST Pickup/Removal Dates: Monday, September 28, 2020 between 9am-4pm EST (Additional Pickup Times on Tuesday Only between 10am - 3pm EST by Appointment Only) After that storage fees of $10.00 per day will be due!
Auction Location: 6110 Edgewater Dr. Suite A, Orlando, Florida 32810 (#1 AUCTIONS Warehouse)
By placing a bid in this auction you agree to the Terms & Details of this sale.
Shipping is available. Please view our shipping page for more information.
15% Buyers premium. If you pickup on the scheduled pickup date by 4:00pm we offer a 3% discount for an all cash payment. In the event you can not abide by the terms and details, DO NOT BID! All items must be removed or have shipping arranged by 4:00pm on the pickup date or they will be considered abandoned & full payment will still be due. Please note that all invoices must be paid on Monday, September 28, 2020 by 4pm. Failure to do so will result in forfeiting the rights to those items and payment will still be due.This auction ends 4 items per minute starting at 8:00pm EST! This will go fast so make sure to get your bids in early and use the max-bid feature so you don't lose out
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