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October 13 is International Skeptics Day. If you don't believe me, google it! You know the internet never lies. 🙂
I haven't been able to find much information on International Skeptics Day (although I did find that it's also celebrated on January 13), so I'm not really sure what kind of skeptic should be celebrated here. Ergo, I'm celebrating the type of skeptic that I am.
You see, the commercials tell me that this shampoo will make me beautiful, that car will make getting groceries as thrilling as a James Bond, and that phone will make me popular. I'm a bit skeptical.
The talk shows and self help books offer five steps to better relationships, health, wealth, and even happiness. While many offer some good tips, I'm still a bit skeptical.
The forwards in my email inbox tell me all sorts of things, but I'm afraid very little of it is true.
For your International Skeptics Day celebration, I suggest reading some of Snopes or watching some Mythbusters. Should be a fun day!
"Immediately the boy's father exclaimed, 'I do believe; help me overcome my unbelief!'"
Mark 9:24


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"The Industry's Best Faculty!"
Sanjeev Katariya
VP, Chief Architect, eBay
As eBay's Chief Architect, Sanjeev Katariya leads global architecture, cloud, AI, computer vision, machine translation, data and platform teams, as well as the overall marketplace technical strategy. In addition to working across various technical leadership teams, his direct teams focus on: AI/ML platforms, computer vision, machine translation and NLP, spatial computing, anomaly detection, general neural network and software research, mobile platform, continuous delivery systems and quality frameworks, cloud security, and marketplace frameworks. Sanjeev spent the bulk of his formative career, 25 years, at Microsoft, dedicated to architecture, science and engineering. He earned a master's degree in electrical engineering from The University of Colorado and a bachelor of science degree in electronics and electrical communications from Punjab Engineering College. He holds more than 32 patents. When not working, Sanjeev enjoys spending time with his wife and three children, and contributes to his personal website, www.sanjeevkatariya.com.

subset: commoncrawl


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If there's a Will, there's a way: Your mental health matters
Will Phillips, Staff Writer
February 16, 2020
Students are starting to realize how important it is to acknowledge mental health issues in college freshman. Over 95% of college counseling centers surveyed by Association for University and College Counseling Center Directors said the number of students with mental health issues is a growing con...
More than a T-Shirt member
Gabi Wy, Features Editor
August 24, 2016
Freshman Clifton Jett wanted to join "everything" college had to offer. The day before classes started, he spoke at the first Christian service offered by university Religious Life. He feels he belongs at the multicultural center, and as a theatre major, he's auditioning for his first U...
'The carrot and the stick'
Armon Siadat
November 11, 2015
Campus Clarity holds rendered ineffective, completion encouraged Rachal Sperka started the required Campus Clarity course Nov. 2—five weeks after the original deadline. Despite receiving an email from the Dean of Students Office stating there would be a hold on her account and she would be unable to ...
SGA population nearly doubles
September 22, 2015
Student Government Association considered 15 applicants for membership Sept. 19, and after a meeting longer than two hours, the organization welcomed 12 new members and almost doubled its population. "I just got interviewed (to be part of SGA)," freshman biochemistry major Gabriela Aguilar said ...
Stages of a semester
Alison Saagar
August 25, 2015
Move in crew welcomes new students by doing their heavy lifting
August 20, 2015
Campus signage confuses some freshmen
Rachel Christian
August 28, 2014
Jaleigh Bugher remembers a time when navigating campus wasn't so easy - something a lot of new students realized this week. Bugher, a freshman pre-radiology major, was on campus with her parents Aug. 21, the first day of Welcome Week. Bugher said it was pretty easy navigating the campus, but it was...
Welcome Week's only sort-of fun for freshmen
Armon Siadat
August 27, 2014
Welcome Week isn't the same when it's not your first year on campus. Each year, various organizations on campus put on events in the days leading up to the first week of classes. As a freshman, Welcome Week was exciting because I had no idea what to expect and my RA really talked it up. At...
Remain fresh: don't be a carbon copy
Ariana Beedie
September 4, 2013
I haven't seen much diversity among the freshmen class. Every year, the first day of classes is the same. Girls wear their club outfits or something they've been saving for months to wear. With guys it varies, but most try to pull the freshest gear they own to sport for the first day of classes. I'm...


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laserlikeSurprised at the percentage that think it's a waste of time. My thought is how could this be? You would learn about the culture of a cruise, different countries. You would learn about the wonders of the ocean, tase exotic foods. You would learn about other countries culture, meet people from around the world,learn about sports culture, etc, etc,etc, worlds without ends. So this is revealing to me.
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No other ball has gained this much hype lately as much as the Kirkland Signature Golf Ball has. As Costco announced the release of the Kirkland Golf Ball, both avid and amateur golfers went crazy to get their hands on it! Want to know why? That's what we're going to reveal today in this Kirkland Signature golf balls review article.
Why Choose Kirkland Signature Golf Balls?
The Kirkland Golf Ball, manufactured by Costco was sold out on its very first release. They managed to sell out on their second release too in spite of having an expanded stock. That explains how much packed with they are. Do you want more reasons? We have more.
Kirkland Signature Golf Balls Review
Key Features
Here we have listed the key features of the Kirkland Signature very briefly.
- Distance
- Spin
- Budget Friendly
- Value for Money
- Energy Transfer
- Sound and Feel
- Performance
- Structure
- Service and more
Premium balls costing from $150 – $200 per dozen seemed pretty costly to man, especially the amateurs. Many had to compromise quality for the cost. Thus, Costco decided to come up with this miracle. They informed the audience before releasing the Kirkland Golf Ball that it is going to be both high-quality stuff as well as relatively cheaper. Surprisingly it costs around $70 a dozen only! Compare on your own now.
Value for Money
Do not be fooled by the price of the Kirkland Golf Ball 2018. Costco has a value for money, for which they cut any unnecessary charges. Who wouldn't buy such Best Golf Balls at this price? It is almost a no-brainer. Thus, even losing some of these might not feel as cost-effective as losing the high-end premium golf balls.
Four Pieces of Merit
The shell of the Golf Ball is composed of Urethane. It is used as a shell in almost all types of high-end premium balls. It offers a very softer feel compared to any other cover substance or blend. However, a backdrop of this is that Urethane covers are more prone to damage because of their soft and delicate nature. A rough and scratched up surfaced golf ball results in compromising the flight of the golf ball and its response to the swing of the golf. Due to over-exposure to the sun, the Urethane might also turn yellow in color, hence get discolored.
See also: Difference between TP5 and TP5x
The exterior of the Kirkland Signature comes with a 360 dimpled design that immensely increases the spin rate as well as the ball trajectory, also allowing to create a penetrating ball flight. Let's dig deeper into the materials:
1. The Core
Kirkland Signature Golf ball has a soft and large core. This feature assists amateurs or players with a slow swing speed to boost the velocity of the ball as it moves away from the golf club. The soft aspect of the core ensures maximum compression while the large core prevents energy from the
2. The Mantle
A common problem that many golfers face is that they feel rock-like feel upon hitting the ball. The Kirkland Golf Ball has you covered there. Its mantle increases the soft feel of the ball at impact while reducing the driver spin. It prevents the golf ball from taking a straight flight up in the air due to back-spin.
3. The Outer Core
The Outer Core of the Kirkland Signature consists of rubber. It affects the feel and the spin of that golf ball. The thick rubber core is designed to provide the utterly desired playability and feel without sacrificing distance.
Sold at such a low price, who would avoid buying a dozen of this miraculous golf balls?
Read: Best Winter Golf Balls 2020
The large core of the Kirkland Golf Ball provides amateurs with slow swing speed with maximum distance. It doesn't at all creates any effect on the control. The structure of this model unlocks the hard stages for the beginners without taking away the crisps for the pros. I bet you can't deny getting a dozen for yourself.
Typical high-end golf balls usually compromise spin when opting for greater distance and vice versa. However, the Kirkland Signature Golf Ball by Costco focused on the structure of the golf ball in such a way that it gets professional spin without compromising a bit a distance and also does not make the control any harder.
Costco's Kirkland Ball might not be as impressive as the premium golf balls such as the Titleist Pro V1, but it performs a lot better than the ones that cost similar to the Kirkland Golf Ball. The distance is pretty decent, better than all those other cheap golf balls but a bit worse than the high-end premium golf balls. However, the ball is consistent with its yardage.
Got some queries about golf ball dimples?
Feel and Sound
Avid, or experienced golfers, can tell you the difference between a good quality and a bad quality golf ball by just taking it in the hands. As soon as I took it in my hands, I could feel the soft-rubbery texture that only comes from the premium golf balls. Cheap range balls never feel so delicate upon touch as much as this Kirkland Signature Golf Ball felt. The golf ball also tends to feel similar as the premium golf balls off the putter.
After the superb feel, sounds also come to the argument. The sound produced after hitting the Kirkland Golf Ball with the face of the golf club is more of a subtle soft sound. It does not cause any rock-like or clicky sound, instead, it makes a more natural sound when stroked.
Read More: How Does Weather affect Golf Balls Distance & Flight?
Great Service
After reading all this, you should have zero doubt on the service that the Kirkland Signature Golf Ball provides. The golf ball is made with a technology that secures that the ball gives a great service until it expires. The large core, the mantle, and the rubbery outer core increase the good experience the user spends with the golf ball. Be sure to give it a try!
Topping in all aspects, the Kirkland Golf Ball only has this one backdrop. As the shell is the made up of Urethane, this already gives one disadvantage to the golf ball. The smoothness of the Urethane shell is very likely to be damaged quite easily. You can experience scratches and other problems on the surface of the ball in a short span of time while playing with it.
On average, this golf ball can only last up to a round of play but however performs great while being played with. You can still play with a damaged golf ball, but you won't get the smooth feel anymore and won't be satisfied by the service.
Costco's Audience
Costco designed the Kirkland Signature Golf Ball with all good intentions. So, they put maximum technologies in it for the pros but however, it might be a cause of worry for the beginners. This golf ball was designed to be a premium ball at a lower price that can advantage the professionals. So, if you are among the disadvantaged players, you can, of course, give it a go but you'll require practice to master it.
Comparing with others
The Kirkland Golf Ball is unarguably way better than any cheap golf balls similar to its rate. So, let's not get into it as it's basically a no-brainer. However, compared with a premium high-end golf ball, like the Titleist Pro V1 golf balls, it performs quite up to expectations. The iron shots battle almost equals among both, though in case of driver shots, it seems that the Kirkland have a low spin. There was a very negligible difference in terms of the wedge shots, as stated, only highly skilled players can spot the difference among them.
Another con for us, but a pro for Costco is that these golf balls are not very available. The Kirkland Signature sells like thunder, so many golfers return with a sad face and an empty hand from the shops. As they are relatively very low in price and give such immense quality in return, nobody can refuse to buy a couple of dozens. If you spot them available in stock, grab some extra, in case you don't get it the next time you need it!
Pros and Cons of Kirkland Signature Golf Ball
Here is a brief list of the Pros and Cons of the magical Kirkland Signature Golf Ball that we arranged for you so that you can analyze it at more ease:
- Great Performance
- Decent Distance
- Good Spin
- Value for Money
- Good Sensation on the green
- Energy Transfer
- Minor lacks in Performance
- Less Durable compared to premium balls
- Discoloration of the Urethane
- Not for mid-high handicapped golfers
Final Verdict…
Undoubtedly the Kirkland Signature Golf Balls cost very cheap according to the features it provides, it is a win-win in every aspect. It is always a wise decision to get them whenever they're available as they sell out really fast for its immense popularity. This golf ball gets so much love for precisely the reason we've discussed above. So, you must give it a try!
See also:
- Callaway supersoft golf balls review
- Best practice golf balls
- TaylorMade Rocketballz Golf Balls Review
- Nitro Ultimate Distance Golf Balls review


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AMG peered down from his mountain watching the Swedish snow flurries
But try as he might, he couldn't silence his worries
But what had him so vexed? What could be the matter?
Why those Finns down in Finland were releasing triple platters!
AMG liked his records concise and he liked them rather short
He simply couldn't abide with the 2 hour sort
"This could catch on. This could be a trend," he mumbled
That would make metal all bloated and jumbled
I must find a way to stop it using whatever tool
So artists will obey my 45 Minute Rule
So AMG pondered on how to get his way
He thought and he thunk all through the day
Then an idea hit him, one that tingled his spinal
He would steal all the CDs and make them use vinyl!
Then albums would be short and without any filler
Leaving the final product that much more killer
So down from his mountain he crept and he crawled
to the warehouses where Compact Discs were mothballed
He took every one of those shiny plastic spheres
Then he smashed the digital recorders while guzzling craft beers
With bags full to busting he slunk away in the fog
laughing "Now they'll be forced to use analog!"
To his fortress of snobbery he rode with CD cargo
And no one yet knew of his diabolic embargo
That night he gloated as CDs roasted in a pyre
Tomorrow they'd all realize with shock and great ire
That he'd changed the music industry and left artists all stunned
Now let's see them swallow that sun!
From all of us to all of you: have a merry merry Christmas!


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Both Group 1 winner HOT SNITZEL and Group 3 winner DOTHRAKI represented the Triple Crown and Snowden Racing team in the Group 2 Linlithgo Stakes over 1200m down the Flemington straight on Derby Day.
It was a great sight seeing them both make their runs together. At the 200m mark you would have sworn they were to finish first and second, however the leader ECLAIRE CHOICE proved a touch too strong with our boys finishing a close second and third.
The pair now have combined earnings of over $2,000,000, a figure which far surpasses their modest price tags of $60,000 (HOT SNITZEL) and $45,000 (DOTHRAKI).
Consistent three-year-old filly DENPURR made the trip down south for a run in the Listed Hybrid Plate over 1400m at Flemington on Melbourne Cup Day. Drawing a barrier in the visitors car park didn't help her cause, however in true DENPURR fashion she displayed a blistering turn of foot to clinch third on the line and score her first top three finish in a stakes race!
Her result leads us to believe that there are more stakes race successes in her future ahead of a happy (and lucrative) career in the breeding paddock.
Following the retirement of our Group 1 Sire's Produce winning filly PEGGY JEAN earlier this year due to injury, the owners agreed to put her in foal in order to sell her at a premium price down the track. Top young sire I AM INVINCIBLE was the sire chosen for her and we're pleased to report that she has passed the 45 day positive pregnancy test earlier this month.
PEGGY JEAN is following in the foot steps of Triple Crown mares CAVALRY ROSE (sold in foal to PIERRO for $650,000), PATRONYME (sold in foal to FASTNET ROCK for $325,000) and FLYING SNITZEL (sold for $400,000 not in foal).
Favourite way to spend time outside of racing?
I am tragic foodie, I love trying new restaurants and bars or frequenting the old favourites with my friends and family. I also thoroughly enjoy chilling out at the beach on a nice day.
Favourite non-Triple Crown horse?
At risk of sounding monotonous, I am a big Black Caviar fan. What a heart!
What do you love about the racing industry?
The excitement and the thrill. You can't beat that feeling when your horse wins, it's indescribable. I also really enjoy following the progeny and/or close relatives of horses I have had something to do with in the past, seeing them at the yearling sales and then the races. Watching pedigree pages grow stronger and develop is very interesting.
Do you have a favourite jockey?
I am a big Hugh Bowman fan – he has a useful knack for getting out of trouble when in a sticky situation.
Do you have a punting approach?
My punting approach is to abstain. I am not great at it!
The best aspect of your job?
Having a varied stable of horses to follow, it's as if I have a share in all of them! Celebrating with owners, watching these horses grow, learn and (hopefully) win races is a fantastic element of the role.
At the end of October Mike and Chris set off on a reconnaissance mission to the UK to attend the Tattersalls Autumn Horses in Training Sale held in Newmarket, England. It was an amazing trip which took in not only the sale but also a tour of Godolphin's Newmarket training operations and an introduction to the Sir Henry Cecil trained Frankel who was the best racehorse of our times (yes, better than Black Caviar) and is now standing at Juddmonte Farms at a fee of AUD$275,000 for every single service. His first crop of yearlings have just gone through the sales ring and averaged AUD$1,000,000 (how we would love to have a couple of those!).
The Tattersalls Autumn Horses in Training Sale has produced graduates such as the 2000 Guineas winner and now international sire Makfi, as well as successful Australian middle and longer distance runners such as Foreteller, Moriarty, Disclaimer, The Offer, Junoob, Kelinni, etc. With Australian breeding respected for its speed genetics, the UK and Europe are equally respected for their stamina genetics so this sale certainly opens up an option for us to pursue horses suitable for targeting our lucrative classic races should our owners wish to go down this route in the future. In order to avoid any potential pitfalls in foreign markets, one first needs to do their research and understand how things operate locally. With the experience and knowledge gathered on this expedition, Mike and Chris now feel very well placed to take their tried and true blueprint for Australian sales over to the UK and make a similarly successful impact when venturing over there.
No 'trip of a lifetime' would be complete of course without a detour to the Rugby World Cup Final on their way back to cheer on the Wallabies versus All Blacks. Whilst the result didn't go our way this time it was certainly a privilege to see two greats of the game in Dan Carter and Richie McCaw play their final matches and also see the Australians mount an enormous comeback in true Aussie spirit against the world's best only to just fall short in the end.
The boys arrived home on Melbourne Cup day to complete their memorable trip and what better way to return than by seeing the team's talented three-year-old filly Denpurr earn her first bit of black type (the first of many we hope!) at Flemington on one of the World's greatest racing days.
Yes, they still send hard copy catalogues! It's an old school industry… However its arrival means that Chris and Mike have a lot of reading to do prior to heading up there to select the next bunch of horses to carry the Triple Crown red. 1016 yearlings will be auctioned over five days, beginning on the 6th of January 2016.
For those of you not on the catalogue mailing list, or are simply more technologically adept, click here to view the online catalogue. You may see a few familiar names on some of the pedigree pages.


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Bethel residents say blasting by builder is rattling nerves
It's shaking up neighbors, who say some of the explosions are rocking their homes and their nerves.
"The house shakes so badly that stuff falls off the wall," Adam Lindie of Reservoir Street said. "You have to worry about the foundation."
Toll Brothers, a national luxury home builder, is clearing the site near the Danbury border to build 62 townhomes expected to sell in the low- to mid-$400,000 range. The development is called Bethel Meadows.
"We want to be good neighbors," said
By the permits, Rossi means the range allowed under the law for blasting. Bethel town officials, who are monitoring the work, agree.
"They're within the parameters of what they can do," said Deputy Fire Marshal Michael Connors . "We come out to monitor the blasts at least once a day, as often as possible, to make sure they're working within state rules."
But some neighbors, like William Scollon of Reservoir Street, don't agree the company is within the allowable range. He questions whether the seismographs that show the strength of the blasts are giving accurate readings.
He said the smaller blasts aren't a problem. It's the big ones that cause the shaking.
Scollon wouldn't mind an increase in the smaller blasts if the large ones could be stopped. "Minimize the charges so the house doesn't shake."
Blasting started a few weeks ago and is expected to continue for at least another two months. The property has lots of ledge and high ridges along the east side, near Saxon Road.
"The windows shook and the whole house moved," Saxon Road resident Warren Curtis said of two large blasts -- one last week and one Tuesday. Curtis said he did not object to the smaller blasts, but he called those two "excessive" and outside the blasting limitations.
First Selectman Bob Burke said he asked the police to send an officer to the site whenever a large blast is planned, to ensure safety.
In response to several neighbors who say the town should do more to control the blasting, Burke said, "Toll Brothers is following the statutes of the state of Connecticut as far as blasting is concerned."
Although the company checking the seismic activity is paid by Toll Brothers, Burke said fire marshals from the state and Bethel monitor their work.
Burke said the state fire marshal has been to the Reservoir Street site several times and is due to return today.
"The town has supported that neighborhood," Burke said, referring to money the town spent to defend itself in lawsuits Toll Brothers filed against Bethel in the past few years.
The site, which was vacant, has been controversial since 2002, when Toll Brothers proposed a 128-unit affordable housing development. Neighbors were strongly opposed, in part because of the extensive blasting required, but also because they feared the development would cause increased flooding and heavier traffic on the busy road.
The town's land-use boards rejected the original proposal on the grounds it was too dense for the property. Toll Brothers countered with lawsuits, which the town lost.
A settlement last fall allowed Toll Brothers to submit an application for a 62-unit housing development. None of the units would be affordable housing, but Toll Brothers agreed to put $960,000 into an affordable housing trust fund the Board of Selectmen will establish.
The application was approved by the town early this year.
Besides the physical effects on their homes, Reservoir Street residents said the blasting is affecting their nerves. "These blasts are akin to some kind of torture, resembling bombing during a war," said Diane Kopta , whose house is nearest to the construction site.
Scott Murphy , who has lived on Reservoir Street since 1961, fears the blasting could mean the loss of some of his family history.
"The wall on the barn is shifting," he said about a structure on his property. "How do you replace something your family built in 1860?"
Contact Marietta Homayonpour at firstname.lastname@example.com or
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What is the evidence of the social media influence of your life
Three billion people, about 40{1ca13a2f165dfac25b292d3d801e555022eb1ac87af9364cca0ec010ddb98ab6} of the world's population, use social media-and according to some reports, we spend an average of two hours each day sharing, liking, writing and updating this device. That means about half a million like and Snap chat photos are distributed every minute.
When social media has a big role in our lives, can we sacrifice the health and well-being of our souls and our time? What exactly is the evidence found?
In a study involving 600 adults, about a third said social media had made them feel negative emotions – most frustrated – and jealousy was one of the main causes. This is triggered by the tendency to compare their lives with others and the first cause is the photos of others who are traveling. A sense of envy leads to a "vortex of jealousy," in which people act in envy by adding similar content that makes them jealous of their profile.
Read Also: where can I get an instagram followers easily?
However, jealousy is not a destructive emotion-it often makes us work harder, according to researchers from the University of Michigan and the University of Wisconsin-Milwaukee. They asked 380 students to look at photos and writings from Facebook and Twitter that could "envy", including uploads on expensive items, traveling for holidays and getting engaged. But the type of envy that researchers find is "begin jealousy," which they call causing people to work harder.
Researchers say spending more time on social media can replace face-to-face interactions, but it can also make people feel alienated.
"Exposure to the idealistic portrayal of peer life brings out the feeling of jealousy and false belief that others are happier and have a more successful life, which may increase the feeling of social exclusion."
It is clear that there is not enough material to draw strong conclusions. However, the evidence points in one direction: social media affect people differently, depending on pre-existing conditions and personalities.
Like food, gambling and many other temptations in modern times, it is possible for some individuals not to be advised excessive use. But at the same time, it could be wrong to say that social media is universally a bad thing, because it clearly brings many benefits to our lives as well.


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By: Jennifer Gherardi
I found myself in a conundrum about learning Italian and it seems to be a common problem for language learners.
My husband is Italian but Australian born and I am a real Aussie Skip (kangaroo). We had travelled to Europe many times as Peter (a winemaker in Australia) was working as "flying winemaker" i.e. flying in just for the eight weeks of vintage in France or Italy.
One day we decided to dive in and buy a house in Europe but could not decide between France and Italy. I made the final vote because "you have relations in Italy" and I thought it would be easier for me to learn Italian.
Jennifer at a gelato shop in Spello
Peter spoke his village dialect from the north but within a few years he became conversant in proper Italian, albeit "rough and ready" as he says.
Instead, my language experience was a tad more problematic.
I had completed some Italian classes and had come top of the class but when I arrived in Italy a few months later… I just froze. I could not think fast enough and with Peter at my side he always came to the rescue… and I sort of gave up.
Jennifer in Spello
After we bought our house in Spello, Umbria, each summer trimester I picked up plenty of single words by immersion but still couldn't put a sentence together. So, the time had come to up-skill.
Learning Italian in a non-traditional way
As a documentary filmmaker, I love research and so I set about researching the best way to learn a language. Many days and weeks on the Internet gave me a good picture of the best track to go down. I was delighted to hear the multiple language learners' (polyglots) techniques for speed learning. They all steered clear of traditional classroom methods.
This reminded me of the pilot course I had done in Accelerated Learning with Japanese. It was easy and fun. Was there this type of course in Italy? The answer was yes and no. Yes, there was just one school in the far north but nothing in central Italy.
In our village, I asked visitors and "expats" (who had summer houses there) as to how they would like to learn Italian. I remember Jim saying his wife was doing classes but as soon as he found out they were expected to do homework he bailed. Many said they wanted to be able to speak but then they rolled their eyes at the thought of the pain!
The hilltop town of Spello
I proposed my idea to them. "What if you could go to classes where you start each class in a lovely room and closing your eyes and relaxing while listening to baroque music…then the teacher reads you a story and then you read it back as a group... then you play games to repeat the key words – and it is all fun and positive… with no homework? They suddenly perked up… "yes, I wouldn't mind that at all!".
So, they were attracted to the principle methods of Accelerated Learning. From my reading of the science, it's quite clear that this methodology ingests the words into the short and long-term memory simultaneously. The mind enjoys learning in this way. This made sense to me, as there were no desks or grammar terms when you learned your first language! The proof was there - this is how you should learn a second language… with some help and classes to speed it up.
Paying it forward, a New Business is Born
Thus, I started to build the plan for Spello.
I found two brilliant language graduates, Manuela and Rachele who had the right personalities to become our specialized teachers but how could they get the special training they would need?
Rachele Taccucci, one of the teachers at Enjoy It
I searched Italy and then the whole world to find someone with the right skill set. Weeks later Daniela Montanger, the founder of the London School of Fast Learning, agreed to mentor our teachers for our classes! Our teacher, Manuela, has some dear friends who supplied the lovely studio for our classes. It is in one of the romantic streets in the village, with the cafés just steps away.
Manuela, one of Enjoy It's local teachers
Imagine staying in the plentiful and wonderful stone apartments throughout the village. Then you could walk the flower-lined streets to start the day with coffee and pastries at Bar Tulia before classes. Heaven - with real bells ringing from the nearby churches!
The Spello studio for classes, as seen by google street view
As an outsider to Spello I can see why it is a perfect place to holiday and learn Italian at the same time. On one hand, there are quite a few expats and tourists if you want to speak English and socialize but equally there are many people in the town who only speak Italian. This is what is so important… in big cities so many people speak English it becomes unnecessary to even try! Spello folk (or Spellani as they are known) are very patient and helpful.
Why Spello?
I haven't told you why we bought our house in Spello when we had the whole country to choose from. Firstly, Spello has the best wine shop and degustation wine tasting in all of Italy (Enoteca di Properzio as per The Lonely Planet Guide). As wine and food lovers we had to find a town with lots of restaurants too. The village life we saw each time we visited was very animated and special and I am still uncovering the many layers and secrets of Spello.
Winding down with a drink at Enoteca di Properzio
It does not take long to feel at home and the shopkeepers, waiters and sommeliers quickly recognise you after just a few days. I love how Gianni knows we like our coffee super-hot. His mother (Tulia) makes all the cakes and pastries every day!
Relaxing at Bar Tulia
Even the police are interesting. If they see a car illegally parked they blow their whistle loudly so you can hear it (if you are in a shop) and if you rush out and move your car, there is no fine. But you might get a frown and finger waggle. I love that! Everyone counts and is important in a town of this size.
There is an extensive list of activities and tourist destinations. I am amazed at the free classical concerts in the churches and free pop concerts at the lower of the 3 piazze.
When you search Spello in Google Images you will come across the amazing flower petal carpets created on the streets for the religious festival, the very famous " Infiorata di Spello". This is just one of the amazing festivals where the community create fantastic events. Traditions are highly valued and real Italian life unfolds before your eyes as Spello was founded in 1 B.C.
The community laying down the Infiorata (flower carpet lining the streets) in Spello
My favourite church is Sant'Andrea where there is one Pinturicchio painting. I recall seeing the monk in brown robes and sandals on a misty day come out to close the doors and I swear I felt transported into the movie "In The Name of the Rose" with Sean Connery.
I have travelled extensively with my work but I often pinch myself at being in Spello surrounded by the smells, sights and sounds of this very Italian life. It's something everyone should experience at least once which is why I chose to share my story here.
If you would like to learn Italian the SMART AND FUN way in a wonderful part of Italy, then we have built the perfect language class for you! And oh …yes, also for me!


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Newark Art and Artists: Prints, Photographs, and Other Works on Paper from the Special Collections of the Newark Public Library (Part II)
Newark Art and Artists:
Prints, Photographs, and Other Works on Paper from the
Special Collections of the
Newark Public Library (Part II)
|Robert Birmelin. The Hand, 1985. From the series, "Half Truths." Etching and aquatint on 3 copper plates. Special Collections Division, Newark Public Library. Library purchase, 2001.
Newark Art and Artists is a two–part exhibition of prints, photographs, books, and other works on paper, that either have Newark as their subject, or are created by artists who have lived, studied, worked, or were born in Newark. The exhibition includes works created between 1800 and 2012, and is drawn entirely from the holdings of the Special Collections Division of the Newark Public Library.
James Edward Jones Remembering, 2007. Linocut with watercolor. Special Collections Division, Newark Public Library. Gift of the artist.
Part I of the exhibition,which ran from October 3, 2012 through January 12, 2013, presented works primarily from the 19th and early/middle–20th centuries. Part 2 of the exhibition, opening Jan. 23, features approximately one hundred works created between 1937 and 2012 by a wide range of contemporary, living artists. In addition to artists most frequently associated with Newark such as Willie Cole, Chakaia Booker, and Jerry Gant, the exhibition presents works by a number of other world–renowned artists with Newark roots, including Robert Birmelin, Barbara Kruger, and George Tice.
Artists in the exhibition include: Ibrahim Ahmed, Manuel Acevedo, Sunmarie Allen, Will Barnet, Herbert Beerman, Robert Birmelin, Tom Bish, Mary Lou Bongiorno, Jerome Bongiorno, Chakaia Booker, Dan Brophy, Eleta Caldwell, Willie Cole, Lisa Conrad, Cicely Cottingham, Roy Crosse, Kevin Darmanie, Evonne Davis, Victor Davson, Donald Farkas, Helen Frank, Jerry Gant, Matt Gosser, Gladys Barker Grauer, Grigory Gurevich, Akintola Hanif, Florian Jenkins, James Edward Jones, Robert Knight, Barbara Kruger, Hal Laessig, Donald Lokuta, Maria Mijares, Nell Painter, German Pitrie, Rocco Scary, Kenneth Schnall, Helen Stummer, George Tarr, George Tice, Bisa Washington, Emma Wilcox, Fran Wilner, James Wilson, and Dmitrii Wright, among others.
Will Barnet. Mary, 1937. Lithograph. Special Collections Division, Newark Public Library. Library purchase, 1938.
The exhibition also includes several works by the late Will Barnet, as a posthumous tribute. Early in his career, Barnet, who passed away in November at the age of 101, taught at the Newark Normal School, which later became Kean University and moved to Union Township.
Sunmarie Allen. Newark City Scene Two, 2010. Intaglio print etching. Special Collections Division, Newark Public Library. Library purchase with funding from the Geraldine R. Dodge Foundation, 2013
Wednesday, January 23, 6:00–8:00pm
7:00pm – Curator's talk by Jared Ash on "Art, Artists, and the Newark Public Library: 110 Years and Counting"
Main Library, Centennial Hall
5 Washington St.
Featuring an informational overview of art–related events, initiatives, and resources for Newark artists at the Newark Public Library, and remarks by the curator about the exhibition.
Lisa Conrad. St. Michael's, 2011. Screenprint and wax on embedded handmade paper. Special Collections Division, Newark Public Library. Library purchase with funding from the Geraldine R. Dodge Foundation, 2012
In recognition of the number of artists in the exhibition who are former faculty or alumni of Arts High School and/or the Newark School of Fine and Industrial Arts, the Library is especially encouraging other alumni and faculty from those schools to attend the January 23rd opening reception.
The History of 3D Film Starring Newark and New York
Wednesday, March 6, 2013, 6:00–8:00pm
Auditorium, Main Library
Emmy–nominated, award–winning Newark filmmakers Marylou and Jerome Bongiorno will screen and discuss their short 3D films on Newark and the Brooklyn waterfront. Inspired by Manhatta, the 1921 avant garde film by artists Charles Sheeler and Paul Strand with text by Walt Whitman, which will also be shown, the Bongiornos have updated the technology to 3D, focused on Newark and Brooklyn and created soundscapes. Their "city symphony" films extend a tradition that includes Walter Ruttmann's Berlin: Symphony of a Great City (1927); Dziga Vertov's Man with a Movie Camera (1929) and Jean Vigo's A propos de Nice (1930).
The Bongiornos will also discuss the history of 3D filmmaking, explaining the technical intricacies of capturing a full cityscape in 3D. Take–away 3D glasses will be provided.
Art Portfolio Review and Resource Fair
Saturday, March 9, 2013, 1:00–4:00pm
Centennial Hall, Main Library
|James Wilson. Rising Down, 2012. Acrylic on canvas. Special Collections Division, Newark Public Library. Library purchase, with funding from the Geraldine R. Dodge Foundation, 2013
In conjunction with the exhibition, the Library will be hosting its second free Portfolio Review and Art Resource Fair. Presented in partnership with the Paul Robeson Galleries of Rutgers–Newark, the portfolio review offers artists the opportunity to have their work reviewed by one of four distinguished curators: Mary Birmingham, Curator, Visual Arts Center of New Jersey; Kevin Darmanie, Founder/Director, Kedar Studio of Art; Evonne Davis, Artistic Director, Gallery Aferro; and Adrienne Wheeler, Owner, Adrienne Wheeler Gallery.
In addition to these curators, review participants and other visitors will have an opportunity to meet representatives from a broad range of art organizations, and discover new places to show art, see art, sell art, and create art in Newark. While there is no fee to participate, artists interested in having their portfolios reviewed are encouraged to reserve a review slot in advance. For reservations and additional information, contact Anonda Bell, Director & Curator, Paul Robeson Galleries, at firstname.lastname@example.com, or 973–353–1610.
|Donald Lokuta, Untitled [Group portrait of neighborhood children and teenagers; Napoleon Street and New York Avenue], 1976. From the series, "Ironbound: Newark, New Jersey." Special Collections Division, Newark Public Library. Library purchase, 1980.
Art and Artist Resources
The Reference Center at the Library maintains a guide to online resources and internet links related to art broken down into several categories including art history, image collections, opportunities for artists, photography, prints, and sculpture. The guide is available at: nplwebguides.pbworks.com/w/page/5673336/Art.
Funding for the exhibitions, portfolio reviews, and resource fairs has been provided in part by the Geraldine R. Dodge Foundation. Additional funding from the Dodge Foundation has enabled the Library to purchase works by contemporary Newark artists for the Library's Fine Prints and Photographs Collection, and to expand the total number of Newark artists represented in the Collection.
|Gladys Barker Grauer. I Wish the Rent Was Heaven Sent, 1992. Color lithograph. Special Collections Division, Newark Public Library. Gift of the Rutgers Center for Innovative Print and Paper, 1993.
Newark Art and Artists will be on view in the Main Library's Third Floor Gallery from January 23 through May 25, 2013. The exhibition and related programs are free and open to the general public during regular library hours, Monday through Saturday. For additional information, please visit www.npl.org,or contact the Reference Division through email at: firstname.lastname@example.com, or by calling 973–733–7779.


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I love what I do. I enjoy looking back at where I've been and am excited about where I am going.
I'm a Kansas Girl that found her heart in Oklahoma over 20years ago. I am a graduate from ECU, where I met my husband an local boy, John Engel. We married in 1995 and from there I followed where he led me and the Army sent us. We have three wonderful children, two boys; Andrew a formal Marine that now lives in Kansas City, Ks., Peyton a Sailor in the Navy currently serving in Kings Bay, GA. And one wonderful daughter, Amelia, that attends ECU.
I have wore many hats over the years, from wife, mom, PTA president, spouse club boards around the world, club scout leader, teacher, substitute, and much more. At this time in my life my husband is retired from the Army and now it is my time to for him to follow me as I grow my career as a Realtor. I love Real Estate! I get to met new people, help them and make a living at it all while getting to live in Ada which is my home town by choice.
Call me! I would love to chat with you about real estate!


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Ami Valo Nei is one of the popular and criticized books by Taslima Nasrin. She wrote and published much poetry in her early literature life. She also protests for equal right of a female in his writing. It became much harmful for his career.
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When she published her most popular novel Lojja, Taslima Nasrin suffered a number of physical and other attacks from people. She had written against Islamic philosophy in his writing. So, some angry Muslim of Bangladesh called a Ban for his writing. Nasrin spent the next ten years in exile in the west after leaving Bangladesh. That time she feels love for Bangladesh and writes her emotions in this book. Download this Bangla Book to find out more about Taslima Nasrin.
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Wed Mar 4
- CLE Credit
- Download to calendar
- print friendly
California Eviction Defense: Protecting Low-Income Tenants 2020
- Pacific Time (US & Canada)
- By: Practising Law Institute
Practising Law Institute
- Source: California
- Homelessness
- Public Benefits
- Pro Bono / Legal Services
- Housing
Full scholarships, registration fee waivers, and discounts are widely available to attorneys and staff working for nonprofit/legal services organizations; pro bono attorneys/volunteers (providing no-fee legal assistance to clients individually or through an organization); government attorneys; judges and judicial law clerks; law professors and law students; senior attorneys (age 65 and over); unemployed attorneys; and others with financial hardships. All qualified individuals are encouraged to apply.
Why You Should Attend
Effective representation can make the difference between a family keeping their home and becoming homeless. The housing crisis is only getting worse in California; the tide of displacement leaves more and more people on the street. Those who are evicted face not only the loss of their home, but also displacement from their community. Successfully defending an unlawful detainer is a profound way to impact a tenant's life; housing instability and homelessness have long-term harmful consequences.
For newer attorneys with limited litigation experience, the fast-paced unlawful detainer is also an excellent opportunity to develop skills; your case may be heading into trial only a month after the summons and complaint are served. This training provides the basic knowledge you will need to effectively defend tenants from eviction, including new tenant protections effective January 1, 2020.
What You Will Learn
• Procedural basics of the limited jurisdiction unlawful detainer case;
• How to draft an effective Answer to an Unlawful Detainer Complaint and spot defects in eviction notices;
• Strategic use of responsive motions to attack faulty notices and complaints;
• Trial skills, including evidentiary motions;
• Basics of anti-discrimination laws protecting tenants, and how to assert a fair housing defense in an unlawful detainer;
• How to craft a settlement agreement that protects your client's rights.
Who Should Attend
All attorneys or law students interested in or currently assisting low-income clients with eviction matters would benefit from this program.
Practising Law Institute8002604754
- Attachment(s): Program_Schedule_and_Faculty_(CA_Evic_Def_20_SF).pdf


text:
Dr Clara Mucci
Clara Mucci asserts, "We don't just get a patient. We get someone who is the last part of a chain of family heritage." This presentation examines the paths that intergenerational trauma takes as it transmits from one generation to the next. Within a framework of psychoanalytic and attachment theory, Clara Mucci elaborates how early relational trauma in the form of disorganised attachment is the most damaging means via which trauma from one generation passes to the next. This is explained by the primary care-giver's incapacity to offer the emotional regulation that is necessary for healthy development. Furthermore, a parent who has adopted the defence of dissociation is likely to have a dissociating child. She proposes that secure attachment mediates against future traumatic effects, by enabling one to negotiate loss and process future traumas. Therapy for intergenerational trauma, she suggests, occurs in the form of enactment, where the trauma of past generations may become expressed. Clinical and theoretic material is drawn upon from her own practice and also from such authors as Giovanni Liotti, Dori Laub, Ilany Kogan, Philip Bromberg and Allan Schore.
Video lecture with slides – 44 mins
Past generations, trauma, loss and moving beyond the victim-persecutor dyad
This presentation builds on Clara Mucci's theoretical discussion in Part I, and considers psychoanalytic treatment approaches for the effects of the traumatic experiences in the lives of parents, grandparents and ancestors. One clinical challenge is the decreasing clarity, symbolisation, narrative formulation of the original trauma as it passes from generation to generation. Somatic awareness or even psychosis may be the only way that it is expressed. Equally striking is a tendency towards victim-persecutor relational dynamics. We will look at the possibility of raising these effects to consciousness, easing symptoms and ultimately going beyond these dynamics into greater psychic freedom.
Video lecture with slides – 50 mins
About the Speaker
Clara Mucci is a psychoanalytically-oriented psychotherapist practicing in Milan and Pescara, Italy. She is Full Professor of Clinical Psychology at the University of Chieti, where she taught English Literature and Shakespearean Drama.


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Find Glaziers in Peterborough on the Peterborough Telegraph directory. Get reviews and contact details for each business including phone number, postcode, opening hours and photos.
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30 years theatre,
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The "being" is the subject of my research and theatre is the way to disclose the knowledge.
Infinite lives, Infinite characters, Infinite frames!
The actor is an extremely courageous human being who is not afraid to deconstruct the EGO, to trust the subconscious and to engage with the higher self through the arts. The self who forgives everything after loving and accepting all human thoughts and deeds. The actor is the transporter of universal information to the public.
In life-as we know it-everything needs a method.
I welcome you to share with me schocking secrets about the passage from the ancient Greeks, method acting, meditation, neuroscience, biogenetics and the relationship of Quantum physics to the theatre.
"To be or not To be ", IT IS NOT THE appropriate QUESTION!


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You became so enamored with my leaves.
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So you left.


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External hackers and insider threats. Customer expectations and government mandates.
Data protection is a complex challenge, and it demands attention at every level of an organization. PKWARE's in-house experts are here to help you stay up to date on best practices, emerging trends, and new resources for enterprise data security.
When it rains, it pours.
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Organizations who hadn't yet come to terms with the GDPR were suddenly faced with the prospect of creating not one, but two new data protection strategies—one to meet the EU regulations, and another to comply with whatever rules would apply in the UK after its exit from the EU was complete. It was hardly surprising when companies in North America and elsewhere began to consider leaving the European market entirely, rather than dealing with the complexity and uncertainly that the legal developments had created.
"I love strong encryption. Strong encryption is a great thing."
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New York's cybersecurity law for the financial services industry, 23 NYCRR 500, took effect on March 1. The law is making headlines not because it creates a heavy new burden for compliance, but because it takes a broader view of information security than any previous state or federal law. As a highly visible attempt to set priorities and minimum standards, the New York regulations have the potential to influence the long-term direction of cybersecurity legislation in the United States.
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In May 2018, the European Union's new General Data Protection Regulation will take effect, forcing companies that do business in the EU to comply with strict new standards for data privacy and security. While it won't have the force of law for another 14 months, the GDPR is already influencing data protection strategies around the globe.
Sometimes, an organization's biggest information security headaches come not from the ill-intentioned, but from employees who are trying to do the right thing. As companies and government agencies create and exchange unprecedented volumes of sensitive data, uncontrolled or "runaway" encryption is becoming a serious concern for organizations around the world.
In a world of proliferating cyber threats and constant data exchange, encryption continues to gain visibility as the single most important tool for long-term information security. In fact, a report from Forrester named data encryption as one of the top global cybersecurity trends of 2017. Now more than ever, individuals and businesses are looking for ways to use encryption to keep their sensitive information safe from data thieves, spies, and other cyber threats.
The DDoS attack that crippled such major sites as Twitter, Paypal, Netflix and Reddit last week shifted the world's attention to the so-called Internet of Things (IoT).
Security experts have discussed the IoT as a target for some time, but the coordinated assault against Dyn, one of several companies hosting the the Domain Name System (DNS), brought the dangers into clearer focus.
To better understand what we're dealing with, a deeper dive into the IoT is necessary. Here's a look at how it works, where the vulnerabilities are, and what can be done to improve the security behind the technology.


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Bangkok, Thailand (4 days) >> Cambodia (7 days) >> Vietnam (15 days) >> Thailand (30 days)
We flew from Delhi to Bangkok just over a month ago now, so what have we been doing since India?
Here is a quick update:
Starting with a few days in Bangkok, we headed over to Cambodia and Vietnam for 3 weeks before coming back to Bangkok and on to South Thailand.
A) Bangkok, Thailand – 3 days
B) Siem Reap, Cambodia – 4 days
C) Phnom Penh, Cambodia – 3 days
D) Saigon/Ho Chi Minh City, Vietnam – 4 days
E) Hoi An & Hue, Vietnam – 5 days
F) Hanoi & G) Ha Long Bay, Vietnam – 5 days
In South Thailand we began exploring the stunning beaches around Krabi such as Phi Phi island, Chicken island, Bamboo island and also Railay beach.
Currently, we are in Khanom on the east coast, where we were lucky enough to see some Albino Dolphins! Next, we will be taking a boat to the islands of Ko Toa and Ko Phangan. Then it is on to Malaysia, Indonesia and our last stop Hong Kong.
We're in the process of writing blog posts for each country (and getting behind with them). So in the meantime, here is a small collection of photos from Cambodia and Vietnam.We already have so many memories and stories to share! Will post another blog soon…


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See Why Mercy Emerges Big Brother Naija 2019 Winner – Who is BBNaija 2019 Winner? Find Out Now
See Why Mercy Emerges Big Brother Naija 2019 Winner – Who is BBNaija 2019 Winner? Find Out Now. The so much loved interesting . Mercy has emerged winner of the BBNaija Winner 2019 edition of Big Brother Nigeria Tv Show contest.
Mercy beat Tobi, Cee-C and Alex to the grand prize of N60, 00000 (Sixty Million Naira) at the final on Sunday night.
Nina was the first to be evicted before Alex left as the second runner-up.
Tobi, a huge favourite, was the next to be evicted in a move that shocked viewers.
Big Brother Naija kicked off with a total of 20 housemates but only five housemates made it to the final week which are Tobi, Cee-C, Alex Nina and Mercy.
Read Also: BB Naija 2019 TV Show Full List Of Contestants – No.14 Will Shock You
Mercy beat other contestants to the crown, emerging the 3rd edition winner of Big Brother Nigeria Tv Show 2019.
Finally Mercy emerged as the new BBNaija Winner 2019 at the big brother Nigeria held this year 2019. Big Brother Nigeria.
The winner of BBNaija 2019 Mercy is to go home with forty five million Naira in cash, A trip for two worth million Naira and also home appliances.
It has been trending that Mike or Mercy will be emerged the award winner of BBNaija Winner 2019 but yet to be officially announced. Now that the BBNaija Winner 2019 is yet to be announced we will urge you to still stay tune as we will bring it to your notice once the BBNajia Winner 2019 is announced.
Mercy Big Brother Naija 2019 Winner PROFILE, BIOGRAPHY AND LIFESTYLE
This publication is basically written to review details about Mercy the Big Brother Naija 2019 winner.
Lamborghini Mercy! Queen of highlights!! – Nigerians react as #Mercy emerges winner on #BBNaija Pepper Dem season. The 26-year-old led the polls with 41.77% votes while the second position, Mike, had 19.94% votes. Congrats to Mercy!
That is just the little information we have gathered about the winner of 2019 BBNaija Mercy.


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|Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase
|In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
|Method for sequencing polynucleotides
This invention relates to the sequencing of polynucleotides. In particular, this invention discloses methods for determining the sequence of polynucleotides arrayed on a solid support.
Advances in the study of molecules have been led, in part, by improvement in technologies used to characterize the molecules or their biological reactions. In particular, the study of the nucleic acids DNA and RNA has benefited from developing technologies used for sequence analysis and the study of hybridization events.
An example of the technologies that have improved the study of nucleic acids, is the development of fabricated arrays of immobilized nucleic acids. These arrays consist typically of a high-density matrix of polynucleotides immobilized onto a solid support material. Fodor et al, Trends in Biotechnology (1994) 12:19-26, describes ways of assembling the nucleic acids using a chemically sensitized glass surface protected by a mask, but exposed at defined areas to allow attachment of suitably modified nucleotide phosphoramidites. Fabricated arrays may also be manufactured by the technique of "spotting" known polynucleotides onto a solid support at predetermined positions (e.g. Stimpson et al, PNAS (1995) 92:6379-6383).
A further development in array technology is the attachment of the polynucleotides to the solid support material to form single molecule arrays. Arrays of this type are disclosed in WO-A-00/06770. The advantage of these arrays is that reactions can be monitored at the single molecule level and information on large numbers of single molecules can be collated from a single reaction.
For DNA arrays to be useful, the sequences of the molecular must be determined. U.S. Pat. No. 5,302,509 discloses a method to sequence polynucleotides immobilized on a solid support. The method relies on the incorporation of 3-blocked bases A, G, C and T having a different fluorescent label to the immobilized polynucleotide, in the presence of DNA polymerase. The polymerase incorporates a base complementary to the target polynucleotide, but is prevented from further addition by the 3′-blocking group. The label of the incorporated base can then be determined and the blocking group removed by chemical cleavage to allow further polymerisation to occur. However, the need to remove the blocking groups in this manner is time-consuming and must be performed with high efficiency.
Similarly, EP-A-0640146 discloses a potymerisation-based technique for sequencing DNA. The technique again requires removal of a blocking group prior to subsequent incorporation of nucleotides.
In the general method of the invention, a target polynucleotide sequence can be determined by generating its complement using the potymerase reaction to extend a suitable primer, and characterizing the successive incorporation of bases that generate the complement. The target sequence is, typically, immobilized on a solid support. Each of the different bases A, T, G or C is then brought, by sequential addition, into contact with the target, and any incorporation events detected via a suitable label attached to the base. In contrast to the prior art methods, the present invention requires the presence of a polymerase enzyme that retains a 3′ to 5′ exonuclease fiction, which is induced to remove an incorporated labelled base after detection of incorporation. A corresponding non-labelled base may then be incorporated into the complementary strand to allow further sequence determinations to be made. Repeating the procedure allows the sequence of the complement to be identified, and thereby the target sequence also.
The use of the polymerase enzyme's exonuclease function in this way is a characteristic feature of the invention. It permits repeated incorporation of labelled bases to take place, without the requirement for separate steps of chemical cleavage or photoblcaching.
Accordingly, a method for determining the sequence of a target polynucleotide on an array, comprises the steps of:
(i) contacting the array with one or more detectably-labelled bases A, T, G and C, under conditions that permit the polymerisation reaction to occur, to thereby incorporate a labelled base into a strand complementary to the target;
(ii) removing non-incorporated bases and detecting an incorporation event;
(iii) optionally repeating steps (i) and (ii) with one or more additional labelled bases, to determine a partial sequence;
(iv) contacting the array of step (iii) with a DNA polymerase having 3′ to 5′ exonuclease activity, under conditions whereby the polymerase cleaves the labelled base(s) and incorporates corresponding non-labelled base(s); and
(v) repeating steps (i)-(iv) sequentially, to determine the sequence.
Sequencing the polynucleotides on the array makes it possible to form a spatially addressable array. This may then be used for many different applications, including genotyping studies and other characterization experiments.
The method of the present invention may be automated to produce a very efficient and fast sequence determination.
The method according to the invention, for determining the sequence of the arrayed polynucleotides, is carried out by contacting the array separately with the different bases to form the complement to that of the target polynucleotide, and detecting incorporation. The method makes use of polymerisation, whereby a polymerase enzyme extends the complementary strand by incorporating the correct base complementary to that on the target. The polymerisation reaction also requires a specific primer to initiate polymerisation.
For each cycle, the incorporation of a labelled base is carried out by the polymerase enzyme, and the incorporation event determined. Many different polymerase enzymes are now known to comprise an exonuclease function which is used to remove mismatches or mutations during normal DNA replication. This function is exploited in the present invention to remove the incorporated labelled base (or bases) after detection, permitting further sequence determinations to be made.
In the context of the invention, reference to the bases A, T, G and C is taken to be a reference to the deoxynucleoside triphosphates adenosine, thymidine, guanosine and cytidine, and to functional analogs thereof, including the chain termination dideoxynucleoside triphosphates which may be used as the labelled bases for the initial incorporation event.
The terms "arrayed polynucleotides" and "polynucleotide arrays" are used herein to define an array of polynucleotides that are immobilized on a solid support material. The polynucleotides may be immobilized to the solid support through a linker molecule, or may be attached to a particle, e.g. a microsphere, which is itself attached to a solid support material.
The polynucleotides may be attached to the solid support by recognized means, including the use of biotin-avidin interactions. Methods for immobilizing polynucleotides on a solid support are well known in the art, and include lithographic techniques and "spotting" individual polynucleotides in defined positions on a solid support. Suitable solid supports are known in the art, and include glass slides, ceramic and silicon surfaces and plastics materials. The support is usually a flat surface. In one embodiment, the polynucleotides are attached to the solid support via Macroscopic beads (microspheres), which may in turn be attached to the solid support by known means. The microspheres may be of any suitable size, typically in the range of from 10 nm to 100 nm in diameter. Attachment via microspheres allows discrete regions of polynucleotides to be easily generated on the array. Each microsphere may have multiple copies of a polynucleotide attached, and each microsphere can be resolved individually to determine incorporation events. Preferably, the arrays that are used are single molecule arrays that comprise polynucleotides in distinct optically resolvable areas, e.g. as disclosed in WO-A-00/06770.
The sequencing method may be carried out on both single molecule and multi molecule arrays, i.e. arrays of distinct individual molecules and arrays of distinct regions comprising multiple copies of one individual molecule. When multi-molecule arrays are used, it may be preferable to use a mixture of labelled and non-labelled base in the initial incorporation step. Diluting the concentration of labelled base in this way ensures that not every complementary strand incorporates a labelled base, and therefore distinct labels can be resolved within the relatively high densities of the multi molecule arrays. Single molecule arrays allow each individual polynucleotide to be resolved separately. The use of single molecule arrays is preferred. Sequencing single molecule arrays allows a spatially addressable array to be formed.
The term "spatially addressable" is used herein to describe how different molecules may be identified on the basis of their position on an array.
The method makes use of the polymerisation reaction to generate the complementary sequence of the target. The conditions necessary for polymerisation to occur will be apparent to the skilled person.
The polymerase required to carry out the incorporation of the labelled bases into the complementary strand (step (i)) does not need to have an exonuclease function and indeed it is preferred if it does not have this function. The potymerase may be removed from the array by a washing step, and a suitable polymerase, comprising an exonuclease activity, brought into contact with the array in a subsequent step, for example on addition of the non-labelled bases. Suitable polymerase enzymes will be known to those skilled in the art, and include DNA polymerase I, the Klenow fragment, DNA polymerase III and T4 or T7 DNA polymerase.
To carry out the polymerase reaction it will usually be necessary to first anneal a primer sequence to the target polynucleotide, the primer sequence being recognized by the polymerase enzyme and acting as an initiation site for the subsequent extension of the complementary strand. The primer sequence may be added as a separate component with respect to the target polynucleotide. Alternatively, the primer and the target polynucleotide may each be part of one single stranded molecule, with the primer portion forming an intramolecular duplex with a part of the target, i.e. a hairpin loop structure. This structure may be immobilized to the solid support at any point on the molecule.
Other conditions necessary for carrying out the polymerase reaction, including temperature, pH, buffer compositions etc., will be apparent to those skilled in the art.
This polymerisation step is allowed to proceed for a time sufficient to allow incorporation of a base. Bases that are not incorporated are then removed, for example, by subjecting the array to a washing step, and detection of the incorporated labels may then be carried out.
In a preferred embodiment, the label is a fluorescent moiety and may be attached to the base in such a way to prevent further incorporation from occurring, i.e. the base is a chain terminator. Many examples of fluorophores that may be used are known in the prior art e.g. tetramethylrhodamine (TVR). The attachment of a suitable fluorophore to a base can be carried out by conventional means. Suitably labelled bases are also available from commercial sources. When the label is a fluorophore, the fluorescence signal generated on incorporation may be measured by optical means, e.g. by a confocal microscope.
Detection may be by conventional means, for example if the label is a fluorescent moiety, detection of an incorporated base may be carried out by using a confocal scanning microscope to scan the surface of the array with a laser, to image a fluorophore bound directly to the incorporated base. Alternatively, a sensitive 2-D detector, such as a charge-coupled detector (CCD), can be used to visualize the individual signals generated. However, other techniques such as scanning near-field optical microscopy (SNOM) are available and may be used when imaging dense arrays. For example, Using SNOM, individual polynucleotides may be distinguished when separated by a distance of less than 100 nm, e.g. 10 nm to 10 μn. For a description of scanning near-field optical microscopy, see Moyer et al., Laser Focus World (1993) 29:10. Suitable apparatus used for imaging polynucleotide arrays are known and the technical set-up will be apparent to the skilled person.
After the detection of an incorporation event, the exonuclease activity of the polymerase enzyme is used to remove the labelled base. The polymerase is usually added separately after the washing step which may have removed the polymerase that incorporated the labelled base. The amount of polymerase required will depend on the amount of polynucleotides arrayed on the solid support, but can be determined readily by the skilled person. A suitable concentration will be 10 nM to 10 μM. In addition to the polymerase, an unlabelled base corresponding to that incorporated may be added. The unlabelled base will not be incorporated onto the complementary strand until removal of the labelled base. The unlabelled base may be a chain-terminator having a removable blocking group or ligand attached. The blocking group must be removed prior to repeating step (i). Suitable blocking groups are known in the art and include photoactivatible ligands.
In the absence of further polymerisation, the polymerase will "switch on" its exonuclease activity to cleave the terminal base on the complementary strand. Cleavage will proceed until a suitable base is available for incorporation, when the more favoured polymerisation reaction will occur. There is therefore an equilibrium reaction occurring with repeated cleavage and (if available) subsequent base incorporation at the terminal end of the complementary strand. Further cleavage, beyond the terminal base, is prevented by the preference of the polymerase for DNA synthesis.
The process of incorporating and removing labelled bases may then be repeated using each of the different bases until the sequence has been determined.
The cleavage/polymerisation steps do not need to be repeated sequentially for each incorporated base. For example, it is possible that several bases are incorporated (and detected) prior to the cleavage step. To illustrate this, the following (template) sequence may be considered.
A first labelled base C may be incorporated onto the template, and S incorporation detected. Further labelled bases A and G may then be added sequentially with detection. It will then be known that CAG forms a partial sequence of the growing strand. The labelled bases may then be removed by the exonuclease activity of a polymerase. The exonuclease activity may be induced by incubating the polymerase with the template in the presence of only the non-labelled base C. This base cannot be incorporated onto the template and so the polymerisation reaction "stalls" and is replaced by the exonuclease activity. The labelled bases are removed until cleavage of the labelled C occurs, thereby permitting incorporation of the non-labelled C. The addition of the other non-labelled bases A and G can then be carried out to re-form the growing strand. The procedure may then be repeated for the next part of the template sequence.
The following Example illustrates the invention, with reference to the accompanying drawing.
A 20 mer target polynucleotide (SEQ ID NO. 2) and a suitable (13 mer) primer molecule (SEQ ID NO. 3) were used having the sequences:
Incorporation of the fluorescent base (TMR-dUTP; obtained from Nycomed Amersham) was performed in a buffer of 50 mM Tris-HCl, pH 7.5, 10 mM NaCl, 2 mM DTT, 1 mM K
After reannealing, BSA, dTTP and T4 DNA polymerase (having a 3′ to 5′ exonuclease function) were added to final concentrations of 0.1 mg/ml, 5 mM, and 500 nM, respectively. The reaction was allowed to proceed at 23° C., and then quenched, at various time points up to 30 minutes, using 0.5 M EDTA.
The reaction mixtures at each time point were resolved by electrophoresis and imaged using a Molecular Dynamics phosphoimager.
The results are shown in FIG.
It can be seen that, during the time course of the reaction, the amount of labelled 14 mer (the 13 mer and incorporated labelled base) is reduced as the labelled base is excised from the polynucleotide and an unlabelled base incorporated. This represents the "idling" of the polymerase and the "switching on" of the exonuclease function. This demonstrates that the exonuclease activity can be utilized to excise a terminal base and, provided that a suitable replacement base is present, the polynucleotide is maintained, ready for further sequencing to occur.


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Mr. Sechie Philip receives Wim Deetman Scholarship
The Grotius Centre congratulates LL.M. Public International Law student Mr. Sechie Philip from Nigeria as one of the recipients of the Wim Deetman Scholarship 2017-2018.
The scholarship is a private initiative by the Wim Deetman Scholarship Foundation, with support from the Municipality of The Hague. The scholarship was presented to Wim Deetman when he left office as mayor of The Hague in 2008. The scholarship is meant for students from developing countries who would like to study for a master's degree in Leiden/The Hague in the area of peace and justice. To be considered for the scholarship students must have obtained good academic results, show an outstanding motivation for their chosen programme and show that they are socially engaged. Recipients are expected to become ambassadors for peace and justice for the city of The Hague.
The ceremony took place at The Hague Institute for Global Justice on 24 October and was opened by Deputy Mayor Saskia Bruines, The Hague's alderman for the Knowledge Economy, International Affairs, Youth and Education.


text: The theory behind treating depression with medications is that happiness is mediated by neurotransmitters and unusually low levels of them cause depression. Serotonin is most the most commonly targeted but dopamine, and epinephrine may also also be targeted. Monoamine oxidase inhibitors are yet another class. The most commonly prescribed drugs are in a class known … Continue reading Depressed Again… Naturally! pt. 3


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Five Ocean Grove bowls teams will enjoy a week off pennant after proving successful in first week of the finals series.
Premier Division action took place on the greens at Highton Bowls Club and the two matches came down to the last end. Ocean Grove finished second on the ladder and took on minor premier Bareena. The prize on the line was a direct path to this season' s decider.
With never any more than 10 shots in the match the Ocean Grove side fought off a fast finishing Bareena team to book that spot winning the match by three shots.
The elimination final was even closer with Queenscliff and Eastern Park level on shots with 100 ends completed. Both team would take to the green for one extra end on each rink. Queenscliff took the honours and will meet Bareena in the preliminary final.
Division Three played out a thriller at Leopold in another qualifying final which also came down to the final bowl.
On a green that looked like it was running magnificently, Ocean Grove skipper Rod Swan delivered the final blow when scores were locked at 92-92 after 99 ends. Swan stood tall, delivered his bowl to rest of the closest bowl to finish only inches from the jack.
Fortunately the Anglesea skipper missed turning the head over and although he made contact and the jack was moved, Ocean Grove still retained the much needed one shot. Swan went on to win the rink 27-11 while Trevor Turnley skipped his four to a 29-18 rink win.
The trip to Bannockburn for Division Seven may have felt long, however the trip back to Ocean Grove would not have felt the same given the side returned with the victory that put it into the grand final.
Keith George, John Aitken, Nancy Quinton and Robin Brache led the way for the Grovers with their team's 26-19 rink win which was also supported by David Harvey's 24-18 result.
Albert Touwslager chipped in for a hard fought two-shot win which was enough to offset the narrow deficit from the Lyn Stonehouse team and earn another crack at the season's biggest prize.
Division Ten was the fourth team that had the chance to advance direct to the grand final and they did that in fine fashion. Both rinks having success on the day with Bob Nicol, Maureen Brooks, John Hoult and Martin VanDoren in cruise control winning 30-13 while Graeme Constable's team finished one-shot winners 24-23.
Two Ocean Grove teams played off in the elimination final. Division One travelled to Geelong Cement to take on Torquay. Ocean Grove side controlled the day from the outset. Luke Benson guided his foursome of Garry Bowyer, Jeff Howarth, Michael Allen to the biggest win of the day 31-11. Equally impressive was the performance of Jane McMenaman, Garry Redford, Brian Smith and Rod Brehaut who recorded a crucial 11-shot win over the dangerous Alan Sampson from Torquay.
Owen Clark 25-20 ensured the margin was enough to cover the narrow loss to Alan Callow's team and overall register the win by 28 shots and book a meeting with City of
Geelong this weekend in the preliminary final.
The Division Six side could not advance any further in the finals series, just going down by 10 shots to Torquay at Eastern Park.
In Midweek Pennant the defending State Champions have one more hurdle before they could again have the chance to defend their crown.
The qualifying final win against Torquay last week saw a reversal of form after succumbing to the same opposition the previous week.
Karina van Weel skipped her side to a 13-shot margin which ensured the major scoreboard would be in Ocean Grove's favour.
Elaine Price was outstanding in her role as lead for van Weel while Faye Richardson took the honours on her rink by four shots 25-21.
Ocean Grove will now meet the winner of City of Geelong vs Torquay.
Four other Midweek teams will this week play off in a preliminary final.
Division Three last week won their elimination final with a dominating performance against Eastern Park. David Rudge, Anne Way, Sandra Murphy and Marianne Rogan in sublime form 35-10. They will meet Anglesea at Torquay.
In a similar position the Sixers also stepped up to keep their season alive with a terrific 24-shot win. Heather Quilty 30-18 and Lorraine Oliver 27-15 guiding the team to the preliminary final against Barwon Heads at Bell Post Hill.
The Division Four side lowered its colours in the qualifying final but will be looking to bounce back this week in the prelim against City of Geelong at Torquay.
Also with the same opportunity to bounce back is Division Seven when they go toe to toe with Geelong RSL at Bell Post Hill.
Division Five could not match it with Portarlington on the day eventually going down in their elimination final.