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Title: History of the Scholars of Shen Zhou, Volume VI
Short Title: Record of 2007
Original Author: Qu Hui
Keywords: history, sosz, forum, scholars, 2007
Description: The sixth volume in a series of articles detaling the history of the forum from 2002-2008.
History of the Scholars, Volume VI (2007)
In the first month, Sun Gongli is voted Scholar of the Month. On the fourth day, the Three Kingdoms Officer Encyclopedia goes online.
In the second month, Dong Zhou is voted Scholar of the Month. On the sixth day, the mismatched ICQ button is fixed. Also on the sixth day, Romance of the Three Kingdoms XI is released in North America for the Playstation 2. On the tenth day, a week of downtime occurs due to a server loss.
In the third month, Kristina is voted Scholar of the Month. On the Ides of March, the April Fool's Day joke begins and lasts until the second day of the fourth month (1).
In the fourth month, there is no Scholar of the Month. On the third day, the auto-pruning feature is permanently disabled. On the seventeenth day, there is a major reorganizing of the forum administration. Dong Zhou and Sun Gongli were added to the moderation team(2), SYL, Kong Wen, Rhiannon, and the Honorable Lady Wu's moderated forums increased by one each (3), Sun Xia and Catalyst's moderated forums increased by three each (4). Ian, Shield of Rohan and Zhang Liao 17 were removed as moderators due to inactivity.
In the fifth month, Mitsunari is voted Scholar of the Month.
From the sixth month onward, Scholar of the Month is no longer awarded (5).
In the sixth month, on the first day, JCC is reinstated as a moderator of Current Affairs. On the second day, Kristina joined the moderation team as moderator of the Literature, Academics and Philosophy forum. On the third day, Kristina compiles the first version of the history.
In the tenth month, the Honorable Lady Wu began translating the Legend of Cao Cao, thus beginning a period of great interest in LoCC and its mods.
In the twelfth month, on the eighth day, Catalyst steps down as moderator. On the ninth day, the forum breaks and is repaired. On the fourteenth day, the Honorable Lady Wu releases the first beta English patch of Legend of Cao Cao.
(1)For more information, please see "History of the April Fool's Day Jokes."
(2)Dong becomes moderator of the Sanguo Yanyi Symposium and Gongli becomes moderator of Dynasty Warriors Training and Gamer's Haven.
(3)Kong is added to Other Three Kingdoms and Koei Games, the Honorable Lady Wu to Dynasty Warriors Training and Rhiannon to Artistic Expression and Illustration.
(4) Both were added to the Pub and the Pub, Timeless Threads, SunXia is added to Other Tree Kingdoms and Koei Games and Catalyst is added to Gamer's Haven/
(5) This can be seen as the end of the Scholar of the Month title, as it would be changed to Scholar of the Quarter soon after.

subset: commoncrawl


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Movie Review: Kick-Ass 2
The following review contains spoilers for Kick-Ass. It also contains adult language, due to the name of one of the characters. Reader discretion is advised.
In my reviews of movies here on the site, one thing I always try to mention is the film's soundtrack. Much as some people writing about Firefly say that the ship is a character, so too is the music in any TV show or film. And sometimes movies that get little or no play can have great soundtracks.
Kick-Ass 2 is one such film.


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Blog Archives
Comedy for Your Shakespeare Lessons
Adam Long, Daniel Singer, and Jess Winfield are the authors of The Complete Works of William Shakespeare. Below is a Q&A Daniel and Jess did with StageNotes.net.
StageNotes: What were your favorite subjects in high school and why?
Daniel: Drama, Concert Choir, Yearbook, Art, English. The arts classes were great because we learned by DOING. Studying English gave me practical writing skills that I use every day. Touch Typing was probably one of the most helpful classes of my entire life.
Jess: English, European History, and P.E. (I was on the basketball team until my Junior year). I enjoyed Drama, but gave it up in favor of the Forensics (speech) team. Same idea of developing skills in performance, delivery, comic timing and the like, but more fun travel, days off from school; plus I knew I'd have a great role because I was choosing the material myself. And I wouldn't have to deal with other pesky actors: I would play ALL the roles!
SN: How did you first become interested in Shakespeare?
Daniel: My 8th grade class read Romeo & Juliet aloud and I instantly loved the verse form of the dialogue. The rhythmic language appealed to me and I didn't have any problem understanding it. When the BBC filmed all of Shakespeare's plays in the late 1970's I watched them all and thought, "Some of these plays are fantastic! (Others, not so much!)" While studying drama in London, I saw everything the Royal Shakespeare Company did. They were so adept at finding clever techniques to make the old plays feel new. Their modern-dress Taming of the Shrew with Jonathan Pryce blew my mind.
Jess: I'd only had the requisite curriculum in Shakespeare (R&J, Midsummer Night's Dream, and Henry IV Parts 1 and 2) and hadn't been wowed by any of it. Then two actors from the Ashland Shakespeare Festival came to perform for our drama class. They did a couple of Shakespeare scenes (which ones, I don't recall), but they also did a bit of the game of "Questions" from Tom Stoppard's Rosencrantz and Guildenstern Are Dead, which prompted me to buy a copy of the play immediately. As it happened, my English class was just starting Hamlet. I found the interplay between the two works exhilarating. So in a way, my entrée to Shakespeare has always been via the backdoor of parody and satire… and Tom Stoppard.
SN: You mention in the notes that the play was originally developed through improvisation and ad lib. Can you please explain how the play came to be?
Daniel grew up in Santa Rosa CA, just up the road from the original Renaissance Pleasure Faire in Marin County. He'd worked there as an actor in the late 1970's. After drama school, he sent the Faire a proposal to produce a half-hour Hamlet – all Faire entertainment was scheduled in half-hour timeslots. There was a surprising lack of Shakespeare in their offerings so they gave the show a green light. Tom Stoppard's Dogg's Hamlet had proven that an abbreviated version of the Prince of Denmark's tragic tale was both easy to follow and comical in its sheer brevity, so it seemed like a natural. Daniel's script was originally just a reduction of the play with no jokes in it.
Two of the actors Daniel hired, Jess Winfield and Adam Long, were brilliant young comics. We were all strongly influenced by the antics of the Marx Brothers, Bugs Bunny, and Monty Python. Our Hamlet became a showcase of broad humor and personal interactions between the actors. This allowed the audience to enjoy the show on multiple levels: the cleverness of seeing the greatest play in the English language rudely compacted into an absurdly short skit; the delight of vaudeville-style slapstick adapted to a 16th-Century idiom; and the witty interplay of three charismatic guys struggling to get through the damn thing.
Keep reading this interview at StageNotes.net!
The Complete Works of William Shakespeare
To celebrate the 20th anniversary of the Reduced Shakespeare Company's classic farce, two of its original writer/performers (Daniel Singer and Jess Winfield) have thoroughly revised the show to bring it up to date for 21st-century audiences, incorporating some of the funniest material from the numerous amateur and professional productions that have been performed around the world.
The cultural touchstone that is The Complete Works of William Shakespeare (abridged) was born when three inspired, charismatic comics, having honed their pass-the-hat act at Renaissance fairs, premiered their preposterous masterwork at the Edinburgh Festival Fringe in 1987. It quickly became a worldwide phenomenon, earning the title of London's second-longest-running comedy after a decade at the Criterion Theatre. The Complete Works of William Shakespeare (abridged) is one of the world's most frequently produced plays, and has been translated into several dozen languages.
Featured are all 37 of Shakespeare's plays, meant to be performed in 97 minutes, by three actors. Fast paced, witty, and physical, it's full of laughter for Shakespeare lovers and haters alike.


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Child in coma after Coraopolis explosion
Investigators say 4 kids started bonfire on Fifth Avenue
A 12-year-old boy has a long road to recovery after being burned in an explosion Monday afternoon.
Neighbors told Channel 4 Action News they heard the blast in the back yard of a home on Fifth Avenue and saw Peyton Brown on fire.
"I just hear my little brother scream, so I run out in to the back yard, and my little brother's on fire from his feet to his head," said Peyton's older brother, Donovan Brown.
"I basically heard an explosion and looked out the window and see everybody going crazy," said Peyton's mother, Georgette Brown.
Investigators said Peyton and his friends -- ages 12 to 14 -- had started a bonfire without his parents' knowledge at their Coraopolis home.
"He kept saying, 'I'm sorry, I'm sorry, I regret it already. You told me not to play with fire, but they did anyway.' He was on fire, and my husband and his friends went out and got his shirt off, and if they didn't do that he would've been so much worse," said Georgette Brown.
Fortunately, a Columbia Gas employee who was nearby rushed to the Browns' backyard and helped put the fire out with his coat and assisted the family.
"We heard a very loud explosion, and we're working for Columbia Gas, and we don't like hearing explosions," said Larry Jones. "We felt it and we saw a plume of smoke."
Jones said a construction crew from Team Fishel used fire extinguishers to put out the other flames and help two other boys who were injured.
"One of the things I said, I said, 'Peyton, this is going to hurt,' because I knew the pain probably hadn't hit him immediately. He just wanted up, and he couldn't understand why he was laying there, and I said, 'Bud, you have to stay still,'" said Jones.
Two of the boys have been released from a hospital with minor burns. Peyton, who suffered second- and third-degree burns, will need more surgery after he awakes from a medically-induced coma.
The boy's condition was upgraded to fair on Monday morning, but his mother said, "I'm scared to death because it is going to be a long road."
"I know he's going to be alright. He's a strong kid," said his brother.


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Are you a DOC NYC Passholder or IFC Center member? Sign in with your pass or member number to access your benefits!
Academy Award Winner for Best Picture, Best International Feature, Best Director, and Best Original Screenplay!
Ki-taek's family of four is close, but fully unemployed, with a bleak future ahead of them. The son Ki-woo is recommended by his friend, a student at a prestigious university, for a well-paid tutoring job, spawning hopes of a regular income. Carrying the expectations of all his family, Ki-woo heads to the Park family home for an interview. Arriving at the house of Mr. Park, the owner of a global IT firm, Ki-woo meets Yeon-kyo, the beautiful young lady of the house. But following this first meeting between the two families, an unstoppable string of mishaps lies in wait.
Official selection: Cannes Film Festival; New York Film Festival


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Home / Bar Stools / swivel bar stools with arms / Top 56 Beautiful Upholstered Counter Stools With Arms Swivel Bar Walmart And Back Leather Backless Nailheads Stoolss Height Backs Steel Gas Pub Inch White Chairs Ebay Stool Set Extra Imagination
Donatella Bianchi Bar Stools November 29, 2017 16:35:30
Alternatively, you may prefer to buy stools with detachable bar stool that tie-on to the seatbacks. This gives you the advantage of being able to change the covers for cleaning or if you fancy a change of look, and can be useful if your stools get lots of use.
Bar Stools - An old tradition: Bar stools are arguably as old as bars themselves. When someone thought of putting up a relatively tall, long table that would serve as a centerpiece for a public house, it would not have taken long to think up appropriate seating. The bar is often at elbow height of a standing man of average height, for the purposes of making things easier for the bartender who serves while standing, and also to draw attention in a room. Its height meant that conventional chairs would leave patrons at chest or chin-height in relation to the bar, which is no way to enjoy some food or drink. When bar counters served as focal points for food and drink as they did in the old days, one really needed one`s hands at the right height. The obvious solution was to go to the bar counter, order food or drink, then finish it off while standing up and leave afterwards. This was fine for the utilitarian purpose of public houses, but soon people started wanting to hang out for longer. In any case, taverns and pubs profited from having customers hang around for more than just a drink or two. Bar stools were invented to provide comfort and in turn generate revenue for the owners. The height meant that the countertop was in easy reach, and the narrowness meant one could seat a large number of people at the long counter. Since then, going to the pub to meet the locals and congregating at the bar for some drinking have become traditional pastimes. It would probably be unthinkable to imagine a world without the age-old practice of chilling out with the guys or gals for a drink after work while resting one`s feet.
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Learning Index >> Pioneers and their practice: a reference guide
Elizabeth Peacock was born in Staffordshire and took private painting lessons in 1900. After conquering ill health she joined Ethel Mairet* in 1917, working as an assistant in her first weaving workshop at Shottery, Stratford-upon-Avon, then moving to Ditchling with her later that year. In c.1918 Peacock joined Molly Stobart, from a farming family, and started to weave on her own; in 1922 the two moved into a new house and workshop called 'Weavers' at Clayton, near Ditchling. In 1923, aged 43, Elizabeth Peacock exhibited at the Englishwoman Exhibition, Central Halls, Westminster and soon gained a reputation for fine work in the inlay technique. As well as spinning and dyeing her own wool and linen yarns (and training others to spin it), she used imported hand-spun cotton and silk. In the 1920s her stoles, furnishing and dress lengths were in demand from international clients, including the couturier Schiaparelli.
In 1931 Peacock co-founded the Guild of Weavers, Spinners and Dyers, and during the 1930s and '40s organised its annual summer schools. From 1940 to 1957 she taught weaving at Reigate and Redhill School of Art. She undertook a major commission for eight banners to hang in Dartington's great hall, Devon (c.1931-7) and, working with her students, wove fabric to clothe replica figures from the Stone, Bronze and Iron ages shown in the Festival of Britain (1951) Retrospective exhibitions of her work took place at West Surrey College of Art and Design** in 1970 and at the Crafts Study Centre, Bath** in 1978.
*Denotes included in the Crafts Study Centre Collection
**Now called Surrey Institute of Art and Design University College, of which the Crafts Study Centre is a part.


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| 9°C Belfast
Immigration Minister James Brokenshire insisted today that a Mauritian student must be deported as her sobbing mother watched on.
The Conservative said all the facts of the case involving 19-year-old Yashika Bageerathi had been considered but were not deemed to be of the "exceptional nature" required for intervention.
A-level student Yashika, whose case has sparked a petition that has attracted around 155,000 signatures, has been detained at Yarl's Wood immigration centre, near Bedford, on a number of occasions and was due to be deported on Mother's Day but her removal was deferred.
Mr Brokenshire remained steadfast on the controversial decision despite pleas from fellow MPs to show "compassion" as he appeared before the Commons Home Affairs select committee.
A tearful Mrs Bageerathi appeared briefly before the committee, breaking down as she told the committee: "I saw her (on) Mother's Day."
She called for the minister to free her daughter from Yarl's Wood, adding "it's not a place for her, it's a place that is cruel".
David Burrowes, Tory MP for Enfield Southgate, who has campaigned for the Home Office to review its decision, said the minister had not considered his representations about the case.
"What I saw was a predetermined decision having been made," he said. "We were concerned in these details that really affect lives that there is proper compassion and fairness."
He added: "We need to look humanely at every individual case at every point right up to the very end. I don't believe this has happened here."
The first of Yashika's examinations starts on May 14 and the final one will be held on June 23, the committee was told.
Lynne Dawes, headteacher of the Oasis Academy Hadley, north London, said the teenager had had a 100% attendance record but, despite continuing to study while detained in Yarl's Wood, her grades have slipped.
She told the MPs she had stood surety for Yashika, who had gone to every signing on session she was required to.
Ms Dawes said: "I'm very, very concerned. I know she is 19 but she is quite a quiet, reserved young 19 year old. She's finding it very, very difficult. I know she is significantly younger than the other women there so she spends most of her time away from the other women in her room."
Promising student Yashika came to the UK to escape a relative who was physically abusive and claimed asylum last summer.
The committee heard that Yashika 's age means she is not protected by deportation rules that protect children sitting examinations.
Mandie Campbell, director general of immigration enforcement at the Home Office, who considered the case, said: "The guidance was designed specifically for children going through an education process."
Mr Brokenshire suggested there would be a risk of Yashika absconding if she was released from the detention centre.
He added: "We are obviously considering this case. Given that I've been clear on saying that I don't think it is appropriate for ministers, and the Home Secretary has equally made that point, for us to intervene therefore we believe that deportation should proceed."


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By Eroscillator
By Lelo
By Lora DiCarlo
By Le Wand
From the clit to the G-spot and beyond, the female body is laden with nerve-rich pleasure centers capable of producing intense – and intensely satisfying – orgasms and intimate experiences. Capable of experiencing pleasure from 11 unique erogenous zones that often comes in multiple waves, there's really no limit to what the female body can do.
Adult toys for women are designed to help femme folks and their partners to explore and amplify the sexual experiences of the female body. Many of these focus on the clitoris (and for good reason), but there are sex toys for women that stimulate nearly every other part of the female body as well.
Between 75 and 80 percent of women need clitoral stimulation to orgasm. Fortunately, there are tons of great sex toys designed to stimulate the clit. Clit vibrators and stimulators are tremendously popular due to their ability to deliver intense, mind-blowing orgasms again and again. From vibrating wands to toys that stimulate the clitoris without even touching it, there's plenty of fun to be had with these toys.
From dildos to vibrators, there are plenty of outstanding toys – powered and unpowered – that can take you to the furthest reaches or orgasmic ecstasy. With so many options, it's important to first ask yourself what you're looking for in the perfect sex toy before you shop. Once you've narrowed down the features you want, your search should be much easier.
Perfect for more intimate, naughtier play sessions, nipple clamps and toys may not be for everyone, but those who love them really love them. They stimulate the nipples by pinching erogenous zones, causing a mildly painful sensation that rapidly turns to pleasure. If you're feeling a bit kinky, nipple toys might be right up your alley.
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If this interests you, check out our selection of Kegel toys.
Most powered sex toys for women are loaded with features and technology meant to make your next play session more comfortable, more intense, and more tailored to your personal preferences.
Here are some features to look for and consider when buying your next adult toy:
Thank you for you interest in our store.
We will notify you by email regarding your request for membership.
An e-mail with your new password has been sent to %email%


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Denizen from Levi's Men's 9.5 Modern Cargo Shorts - Rafter 32, Beige
Browse all deals at Target
See more in Shorts
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* This offer may contain time-sensitive information and offers. Please check with Target to confirm actual price & availability.
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Under a new law that takes effect in 2011, California middle and high school students must be vaccinated against pertussis (whooping cough). Beginning July 1, 2011, all students entering 7th through 12th grades in both public and private schools will be required to show proof of a "Tdap"1 booster shot before starting school. This requirement applies to all public and private schools. The new requirement was signed into law earlier this year by Governor Schwarzenegger.
"With more than 7,800 confirmed cases and the deaths of 10 infants, California experienced its worst outbreak of Pertussis in more than 50 years," said California Department of Public Health (CDPH) Director Dr. Mark Horton. "Protecting the health of California's youth is one of our most urgent priorities. This new legislation will help us achieve our goal."
California's parents are being urged not to wait for the new fall school year to get their kids vaccinated, since there will be no grace period and the regulations will be strictly enforced. CDPH recommends that parents have their adolescent children vaccinated now in order to protect them against pertussis and to meet the 2011-12 school requirements. Parents should be aware that the protection from childhood immunization to pertussis wears off, and adolescents may be at risk to the highly contagious disease without a booster.
In addition to the Tdap booster, there are several important vaccines recommended for preteens and teens including the meningococcal vaccine, a second chickenpox shot (if they never had chickenpox disease), and the HPV vaccine series. Everyone older than six months old is recommended to receive a seasonal flu vaccine.
Footnote: 1 The Tdap booster is Tetanus toxoid, reduced diphtheria toxoid and acellular pertussis vaccine.
Visit ShotsForSchool.org for more information or www.cdph.ca.gov.


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Check out our flagged ONS jobs below!
JOB TITLE:Pediatric Procedural Sedation Nurse
LOCATION: Geisinger Medical Center Hospital
DEPARTMENT:Pediatric Sedation Services
WORK SCHEDULE: Days
WORK TYPE: Part time .5 FTE 40 Hours Biweekly payperiod
Assesses, plans, organizes, performs and evaluates nursing activities to meet the needs of pediatric patients receiving procedural sedation. Provides health education to parents and patients pre and post sedation. Initiates and interprets hemodynamic monitoring parameters. Coordinates efforts of the multidisciplinary team in planning patient care. Evaluates effectiveness of sedation plan. Collaborates with department secretary to schedule procedures. Reviews patients' medical record for sedation risk assessment criteria. Participates in the team's Performance Improvement activities.
Performs duties to provide the highest level of patient care and maximize patient
satisfaction in accordance with the goals and objectives of the Geisinger Health System.
Reports directly to the Operations Manager.
Demonstrates the ability to be self-motivated and be able to work independently.
Demonstrates the ability to work collaboratively with an interdisciplinary team as well as in a 1:1 nurse-physician team.
Demonstrates the ability to be confident, personable and function with tact and poise in stressful situations.
Demonstrates the ability to be capable of making rapid and clinically accurate decisions and possessing innovation under adverse conditions as well as in potentially critical situations.
Demonstrates the ability to maintain confidentiality.
Demonstrates the ability to interact professionally on all levels within and outside the organization.
Demonstrates compliance with the established Pediatric Service Standards.
Graduation from an approved school of nursing required.
RN currently licensed (or eligible) in the state of Pennsylvania required.
Current PALS certification and IV certification required.
Documented experience and skills with managing central lines required.
Minimum of three years of pediatric experience required. A minimum of one year of current pediatric critical care experience preferred.
Moderate Sedation Certification required within 1 year of hire
Work is typically performed in a clinical environment.
The specific statements shown in each section of this description are not intended to be all-inclusive. They represent typical elements considered necessary to successfully perform the job.
OUR PURPOSE & VALUES: Everything we do is about caring for our patients, our members, our students, our Geisinger family and our communities. KINDNESS: We strive to treat everyone as we would hope to be treated ourselves. EXCELLENCE: We treasure colleagues who humbly strive for excellence. LEARNING: We share our knowledge with the best and brightest to better prepare the caregivers for tomorrow. INNOVATION: We constantly seek new and better ways to care for our patients, our members, our community, and the nation.
GEISINGER MEDICAL CENTER: (GMC), located Danville, Pa., the largest tertiary/quaternary care teaching hospital in the region, is licensed for 475 beds, including 89 pediatric beds in the Janet Weis Children's Hospital. GMC, a Magnet Hospital, maintains the region's only Level 1 Regional Resource Trauma Center with additional qualifications in pediatrics. GMC offers an array of highly specialized medical and surgical services, including neurosciences, cardiovascular services, transplantation, women's health (in the Women's Pavilion), oncology and orthopaedics. The Hospital for Advanced Medicine serves as an integrated center for the most critical patients. Outpatient services are available on and off campus.
ABOUT GEISINGER: Geisinger is a physician-led health system comprised of approximately 30,000 employees, including nearly 1,600 employed physicians, 13 hospital campuses, two research centers, and a 583,000-member health plan Geisinger is nationally recognized for innovative practices and quality care. Geisinger serves more than 3 million people in central, south-central and northeast Pennsylvania and also in southern New Jersey with the addition of National Malcolm Baldridge Award recipient AtlantiCare, A member of Geisinger. In 2017, the Geisinger Commonwealth School of Medicine became the newest member of the Geisinger Family.
We offer healthcare benefits for full time and part time positions from day one, including vision, dental and domestic partners.* Perhaps just as important, from senior management on down, we encourage an atmosphere of collaboration, cooperation and collegiality. For more information, visit www.geisinger.org, or connect with us on Facebook, Instagram, LinkedIn and Twitter.
** Does not qualify for J-1 waiver. We are an Affirmative Action, Equal Opportunity Employer Women and Minorities are Encouraged to Apply. All qualified applicants will receive consideration for employment and will not be discriminated against on the basis of disability or their protected veteran status.
*Domestic partner benefits not applicable at Geisinger Holy Spirit.


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Kate Bosworth and Zooey Deschanel at the Sundance Film Festival 2011 2011-01-24 11:59:05
Sugar Shout Out: Sundance Style Starring Zooey Deschanel, Kate Bosworth, and More!
- Sundance style: Elizabeth Banks, Kate Bosworth, and more dish about staying warm and chic
- Hot or not: Are these celebrity crushes cringe-worthy or cute?
- Should pregnancy equal special parking privileges?
- End of the (beer) line
- Tabatha Coffey shares her Shear Genius with Bella
- Artist Richard Phillips captures Kristen Stewart and Rob Pattinson in his paintings
- Who said it: Michael Scott or Mike "The Situation" Sorrentino?
- Visit Pop tomorrow at 5:30 a.m. PT to watch the Oscar nominations live!
- It's only temporary: Our job-hunter takes on a quick-fix job
- 10 reasons to step it up with step aerobics
- Find out what might be making your dog snore
- Weigh in: Would you rather give up the Internet or food?
- Savory, Spanish-style stuffed peppers
- James Franco gets into a hairy situation at Sundance!


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Additional information
|Metric Volumes
For the month of September, we are open for online orders and pickup only as we are moving to a new location. Please refer to
for hours and pickup location. Currently Pickups will be at 1555 McDonald Street, Unit E. Times Are listed on the website and will change daily. Do not assume it will be ready the same day or next day, wait until you receive the email indicating its ready for pickup. In most cases it should be next day and occasionally the same day depending on what you've ordered. Thinks are a bit chaotic right now.
We apologize for any inconveniences this may cause.
Prairie Brew Supply


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2011 Group 2 Project
--Mark Hill 13:37, 28 April 2011 (EST) There will be problems with saving your work if very new references (2011) are added to the project page.
Gap Junctions
Direct interactions of cells with their neighbouring cells or extracellular matrix at particular site of contact are called cell junctions. [1]
Cell junctions can be categorized functionally into three groups:
1. Occluding junctions
2. Anchoring junctions
3. Communicating junctions
Occluding junctions (Tight junctions found only in vertebrates) fasten the adjacent epithelial sheets to avoid small molecules leakage from one side of the sheet to the other.
Anchoring junctions link the cytoskeleton of a cell to cytoskeleton of its neighbours or to the extracellular matrix. Main types of anchoring junctions in vertebrate tissues are Adherens junctions, Focal adhesions, Desmosomes and Hemidesmosomes. [1]
Gap junctions or communicating junctions are clusters of intercellular membrane channels that directly interact with the cytoplasm of adjoining cells. The topic of interest for this project is on Gap Junctions thus, the remainder of the discussion on this page will mainly be focused upon gap junctions.
In vertebrates, gap junction channels are composed of connexins, a large family of proteins containing approximately more than 20 members. Connexins (Cx) are named after their molecular weight and they are tissue-specific and even cell-specific which represent that some connexins are prominently expressed only in a few tissues and some, like Cx43 being more prevalent. [2] Six connexins oligomerize to form a connexon or hemichannel. [3] Connexons contain either a single type of connexin (homomeric) or several connexins (heteromeric). In addition, adjacent cells may comprise of identically or differently composed connexons hence forming a homotypic or heterotypic intercellular channels. [4]
The structure of gap junctions is to be discussed in detail after a brief history on gap junctions.
Functionally, gap junctions are important in generating electrical impulses in electrically excitable tissues such as heart, smooth muscle and some neurons. In non-excitable cells and other general aspects, they are reviewed to play a key role in electrical coupling and provide a pathway for sharing and selective exchange of metabolites. [5]
Likewise, the functional role of gap junctions will be elaborated further followed by the distribution of gap junctions in different tissues.
Following the discussion on location of gap junctions, a table comparing between different junctions will be constructed as a summary of information concerning various junctions.
Lastly, we will attempt to discuss some of the diseases that may be associated with the mutations or disruptions of different connexin genes and recent research with regard to gap junctions.
1959: Furshpan and Potter report that electrical stimulation that is insufficient to generate and action potential still allows current transfer between some nerve cells. [6]
1962: Dewey and Barr coin the term "nexus" to describe an intercellular connection between smooth muscle cells. [7] This term is now used interchangeably with the term "gap junction"
1963: Loewenstein and Kanno use microelectrodes and flurescent tracers to analyze the membrane permeability of epithelial cell junctions on Drosophilla salivary glands. The find that the junctional membrane surface is highly permeable so that small ions and fluorescent markers can move freely from one cell to the next.[8]
1963: Using thin slices of permanganate and osmium-fixed material, David Robinson discovers an array of hexagonal subunits in electrical synapses of Mauthner cells of goldfish. [9]
1963-1967: Difference between gap junction and tight junction remains unclear and confusing.[10]
1967: Karnovsky and Revel use a lanthanum salt preparation on tissues to reveal the existence of hexagonal intercellular junctions in sections of mouse heart and mouse liver.[11] The lanthanum tracer penetrates a 2-3 nm gap between cell membranes for gap junctions, yet is not able to penetrate the firmly fused tight junctions.[12]
1970: Freeze cleaving electron microscopy techniques conducted by McNutt and Weinstein revealed that each of the two membranes which formed the gap junction could be split into lamellae, one neighbouring the cytoplasm, the other neighbouring the extracellular space.[13] Goodenough and Revel showed that the gap junction was characterized by 2 hexagonal subunits. [14]
1972: A method for isolating gap junctions from mouse liver using the detergent sarkosyl and X-ray diffraction is developed by Goodenough and Stoeckenius. [15]
1974: Connexin is chosen as the family name for the major gap junction proteins that were related but not identical in different tissues.[16]
1975: the term connexon is coined to describe a hexagonal subunit that spans the plasma membrane and forms half of the gap junction, along with a second connexon from an adjacent cell. [17]
1977: Crystallographic analysis of X-ray diffraction images make it possible to obtain a relatively detailed image of connexon structure. [18]
1979: Spray et al. show that gap junction channels in early amphibian embryos are voltage gated. [19]
Early 1980s: Development of ultra rapid freezing techniques allowed us to directly freeze cells and thus view gap junctions instantly at the moment of freezing.[20]
1983: Immunocytochemical localization is used to identify a specific type of gap junction protein. Anti 26K antibodies were localized to the liver gap junction protein (26K)[21].
1985: Manjunath and Page use optical diffraction with negatively stained isolated rabbit heart gap junctions to show six protein subunits of identical molecular weight forming the connexon. [22] These subunits each correspond to a connexin molecule. [23]
1986: Kumar and Gilula develop, clone, and characterize two recombinant cDNA probes coding for gap junction proteins, one for rat liver and one for human liver. [24] Different connexins continue to be discovered using cDNA clones from many tissues.[25] [26]
1987: At the Gap Junciton meeting held in Asilomar, scientists decide to adopt a nomenclature system for connexins which distinguishes connexins on the basis of species of origin and molecular mass in kiloDaltons. [27]
1991: Stauffer et. al develop a method involving cDNA which is able to isolate and purify intact connexons[28]
1995: 13 members of the connexin family in rodents have been identified. [29]
1995: The first connexin defect is described by Reaume et al. who observed that neonatal mice lacking connexin 43 suffered from cardiac malformation. [30] [31]
1997: Kelsell et al. identify a mutation in a gene encoding a the gap junction connexin protein connexin 26 which causes autosomal dominant deafness. [32]
1999: Unger et al. determine the structure of a recombinant cardiac gap junction using electron crystallography. [33]
2002: After screening both mouse and human genomic databases, Willecke et al. determine that there are 19 connexin genes in the mouse genome and 20 in the human genome. [34]
2004: New methodologies that use the activation of certain protein kinases show that phosphorylation of certain amino acids in the C terminal region of a connexin can lead to changes in gap junction communication, as well as regulate connexin trafficking, assembly, disassembly, degradation, and the opening and closing of the channel. [35]
2005: Valiunas et al. show that short interfering RNAs (siRNA) can move from one cell to an adjacent cell via a gap junction if the correct connexin is a part of that particular gap junction. [36]
2006: Bugiani et al. demonstrate that mutations in the GJA12 gene which cause loss of function in connexin 47 cause Pelizaues-Merzbacher-like disease, a disease which manifests itself as a permanent lack of myelin deposition in the brain. [37]
2008: Mutations in the gene GJA1 which codes for connexin 43 can cause Oculodentodigital syndrome (ODD) which is characterized by abnormal development of the face, eyes, limbs, and teeth. Immunofluorescence analysis of adult tissues show that these areas affected by ODD are high in the expression on connexion 43. [38]
2010: The connexin gene family is made up of 20 genes in the mouse genome and 21 genes in the human genome. 20234156
2010-2011: Research relating to gap junctions continues to exist, with over 1300 articles relating to gap junctions turning up on an April 22, 2011 Pubmed search for "gap junction". [39]
Electron Micrograph Filtered Image of 2 Connexons
Micrograph of a negatively stained, isolated gap junction. (a) Low magnification view (x 90,000) of an isolated junction plaque about 0.7 pm across with hexagonal lattice extending throughout plaque. Higher magnification view (x 310,000) of this junction (b) shows that hexagonal packing of the unit is highly ordered with a lattice constant of about 85 A. Its optical diffraction pattern (c). Calculation of a filtered image of the junction (d) from the micrograph shows the average distribution stain about the connexons.
An Electron Micrograph of an Isolated Gap Junction (x 200,000)
Gap junctions are formed by two paired hemichannels, more commonly known as connexons, which come together to provide a direct connection between the cytoplasms of neighbouring cells. [40] [41] The two paired connexons come together to form a hydrophilic channel (1.5 nm diameter) between the two cytoplasms which allows for the passage of small molecules such as ions, second messengers, and small peptides between the two cells. [42] [43] [44] These channels cluster together to form plaques of thousands of units.[45] In order for the two paired connexons to form a sealed channel, the two neighbouring cells must be brought extremely close together.[46] This forms a 2-4 nm intermembrane gap.[47] Each connexon is further made up of 6 protein molecules known as connexins. [48] Thus, a gap junction channel is made up of a total of 12 connexin molecules. In terms of molecular structure, a connexin folds into a four helix structure. [49]
Most cells express multiple types of connexins. These connexins can come together to form connexons that are either homomeric (made up of the same type connexin) or heteromeric (made up of multiple types of connexins). [50] [51] Furthermore, when two identical connexons join up, it is known as a homotypic channel. When two connexons made up of different connexins come together, it is known as a heterotypic channel. [52] Although many connexins are able to form heterotypic channels, not every single combination is permitted. Rather, whether or not a particular heterotypic channel is able to be formed depends on certain factors such as the expression of connexins in adjacent cells. [53] [54] The diversity in the different channels generated by the many different types of human connexins is important because it allows for the generation of different physiological properties, discussed in more detail in the "Function" section. [55]
Simple Cartoon Structure of Gap Junction
A tangential section through a mouse heart reveals the hexagonal gap junctions.
Functional role of gap junctions
Gap junction communication plays a principal role in maintaining cellular homeostasis by providing a pathway for exchanging small, hydrophilic molecules including glucose, glutathione, glutamate, cyclic adenosine monophosphate (cAMP), adenosine triphosphate (ATP), inositol trisphosphate (IP3), and ions like calcium, sodium and potassium. [56]
The permeability of gap junctions depends on the composition of different connexin types. For instance, it was reviewed that Cx 43 channels are 120-160 fold more permeable to ADP and/or ATP than Cx 32 channels. Additionally, heterotypic gap junctions allow for special permeability characteristics compared to homotypic gap junctions. [57]
[58]Gap junction is also important for:
1) Transmission of excitation in cardiac muscle, smooth muscle and central nervous system (CNS) neurons;
2) Signalling in avascular and/or uninnervated tissues;
3) Control of cell growth and oncogenic transformation and
4) Regulation of early developmental events
The mechanisms of the functional role of gap junctions described above are explained further below.
Coupling through gap junctions in electrically excitable cells such as some nerve cells allow for the action potential to spread between the cells rapidly and accurately. Similarly, gap junctions in smooth muscle cells and cardiac muscle cells are responsible for peristaltic movement of the intestines and coordinating rhythmic contractions respectively. [59]
The signalling role of gap junctions can be exemplified in the liver. In normal physiological conditions, low levels of glucose in blood amount to the release of noradrenaline from the sympathetic nerve fibres which trigger the liver cells to break down glycogen and release glucose into the bloodstream. Neural stimulation of hepatocytes give rise to generation of signalling molecules such as inositol (1,4,5) -trisphosphate and release of Ca2+ from intracellular stores. However, hepatocytes at the venous end of the lobule are not directly innervated by sympathetic nerve fibres. Thus, gap junctions provide a pathway for those signalling molecules to be transported to uninnervated hepatocytes for glucose mobilization and release. [4]
Connexin 32 (Cx32) is highly expressed in hepatocytes and the review on the experiment concerning Cx32 knockout (KO) mice shows vulnerability to mutations and increase in incidence of hepatic tumors. The rate of cell growth was also remarkably increased in those Cx32 KO mice. These findings support the idea that inhibitory signals passing through intercellular channels of gap junctions may contribute to control of cell proliferation. Therefore, connexin genes of gap junctions might act as tumor suppressors. [4]
The development of ovarian follicles and the production of fertile oocytes depend on gap junction mediated intercellular communication particularly between the oocyte and the surrounding granulosa cells. Cx37 containing gap junctions exist between the oocyte and the granulosa cells whereas granulosa cells are coupled to each other by Cx43 containing gap junctions. [60] The granulosa cells infer the loss of gap junction interaction between the oocyte and granulosa cells as equivalent to ovulation thus, granulosa cells start to luteinize. This may represent that in normal follicles, inhibitory signals are transmitted through gap junctions from the oocyte to granulosa cells to prevent luteinisation until ovulation has occurred. Thus, communication via gap junctions seems to offer bidirectional signalling system to regulate follicle growth, oogenesis, ovulation and luteinization. [4]
Gap junctions have various functions depending on different types and properties of connexins and their unique expressions, distributions and co-interactions with each other in different tissues. Hence, the role of gap junctions may not only be limited to aforementioned functions and can be explored further.
Gap junctions are composed of connexons. These connexons are located in almost all tissues except in sperm cells, red blood cells and skeletal muscle. In each location gap junctions provide a signalling pathway for neighbouring cells.[61]
Gap junctions are located in the intercalated disks where they are involved in the electrical stimulation of the heart. Gap junctions are responsible for passing electrical impulses between cells creating a heart beat.[62]
Nervous System
The gap junction protein, connexin32 is located in Schwann cells in the PNS and oligodendrocytes in the CNS. Connections are formed between neighbouring cells helping to control electric signals. Gap junction mutations lead to myelination defects.[63]
Smooth Muscle Cells
Connexin 43 and 40 are expressed in vascular smooth muscle where they have permeability and barrier properties. [64] Connexion 43 is a myometrial gap junction protein and it is increased prior to delivery. Gap junctions are important in communication leading to contractions of uterine smooth muscle cells during during birth. [65]
Oocyte and follicular cells
Connexion 43 is located at the top of the oocyte and is important for oocyte development. Follicles lacking this connexin produce ineffective oocytes. The gap junctions are an important charcterisic involved in folliculogenesis. [66]
Ear and Eye
Connexin26 and connexin 30 form gap junctions in the cochlea, mutations in these proteins leads to human deafness. [67] Gap junctions are also found in the retina in amacrine and ganglion cells, they are involved in signal processing.[68]
Comparison with Other Junctions
|Name of Junction
|Associated Proteins
|Tight Junction
|Tight junctions are the most apical junctions of epithelial cells and are important in sealing neighbouring cells together. They form a permeability barrier between individual cells controling the transport of solutes, immune cells and drugs. Tight junctions also prevent the mixing of apical and basolateral components which helps to maintain cell polarity.[69]
Tight junctions are important in transcription, tumor suppression, cell proliferation.[70]
|Intestinal epithelial cells & endothelial cells [71]
|JAMs/Junctional Adhesion Molecules, occludin & claudins [72]
|Inflammatory Bowel Disease (IBD)
which includes Crohn's Disease and Ulcerative Colitis. These diseases are are associated with loss of the tight junction and alterations of tight junction proteins.[73]
|Adherens junctions are composed of a family of cadherin proteins. E-cadherin plays an important role in cell-cell adhesions and for the organisation of the intestinal epithelial barrier.[74] Adherens junctions control endothelial permeability via an opening and closing mechanism. Vascular endothelial cadherin, VE-cadherin, is responsible for epithelial integrity and for the maintenance of endothelial cells. It also controls cell proliferation, apoptosis and is important during embryonic angiogenesis.[75]
|Intestinal epithelial lining & Endothelial cells [76]
|Cadherins & Catenin[77]
|Crohn's Disease
Impaired E-cadherin in the small intestine and colon is associated with loss of homeostasis, barrier dysfunction and infections of enteropathogenic bacteria.[78]
|Desmosomes are responsible for maintaining stable cell-cell adhesion and preserving mechanical strength.[79]
Desmosomes provide anchorage sites for intermediate filaments by attaching neighbouring cells together . They are responsible for barrier functions of the epidermis and mucous epithelia. Although desmosomes are critical for cell–cell adhesion, they contribute to morphogenesis and stabilising the tissue architecture.[80]
|Found in cells subject to mechanical stress, such as the myocardium, bladder, gastrointestinal mucosa, and skin.[81]
|Pemphigus & Bullous Impetigo
Desmosomes contain the desmogleins (Dsg) and desmocollins (Dsc). Pemphigus is an autoimmune disease of the skin and mucous membranes. Dsg1 and Dsg3 are two isoforms in the skin and mucous membranes which are targeted autoantibodies. Dsg1 is also targeted by a toxin which is released by Staphylococcus aureus in Bullous Impetigo leading to blistering.[83]
|Epithelial cells use hemidesmosomes as way of attaching to the connective tissue[84]
Hemidesmosomes are responsible for attaching epithelial cells to the basement membrane which maintains cell integrity. Hemidesmosomes are involved in signal transduction and assist in the organisation of the cytoskeleton, apoptosis, and differentiation.[85]
|skin, the cornea, parts of the gastrointestinal and respiratory tract[86]
|Integrin proteins[87]
|Epidermolysis Bullosa[88]
Defects in hemidesmosomal proteins can lead to blistering diseases of the skin.[89]
|Neuromuscular Junction
|The neuromuscular junction is a synapse, which is an interaction formed between neurons or between a neuron and a specific cell. Communication occurs by neurotransmitter release from the presynaptic membrane which then activates receptors on the postsynaptic membrane. The NMJ uses different neurotransmitters in different organisms; in vertebrates, acetylcholine (ACh) causes excitation and muscle contraction. In others at excitatory NMJs, ACh causes muscle contraction, whereas at inhibitory NMJs GABA is released to cause muscle relaxation.[90]
|occurs between motor neurons and skeletal muscle fibres[91]
|Rapsyn [92] & Cortactin [93]
|Myasthenia Gravis
This is an autoimmune disorder where neuromuscular block occurs. This block is caused by a reduction in the response by the motor end plates to the transmitter, and as well a decrease in the number operating end plates. Resulting in muscle fatigue.[94]
Lambert-Eaton Myasthenic syndrome This disease is associated with defective neurotransmitter release in the neuromuscular junction resulting in impaired muscle function[95]
Diseases Associated with Gap Junctions
Connexin function and expression may be altered by a variety of factors such as hormones and cytokines (for example, tumour necrosis factor-α) and lead to a disease state. However, one of the most important mechanisms that regulates gap junction function is the increase or decrease in the phosphorylation state of individual connexins by a variety of proteins. This leads to mutations in different connexin isoforms which are associated with a range of human diseases. [96]
Deafness/ Hearing Impairments
Connexin Mutations: Cx26, 30, 31, 32, 43
Description: During normal reception of sound, potassium ions are circulated from sensory hair cells to the fibrocytes in the cochlea and back to endolymph via gap junctions, maintaining the potassium/sodium endolymph balance. Mutations of the connexins occlude the gap junction channels or prevent the normal assembly of the channels which block the spread of potassium ions through the junction. Recessive transmission is the most common inheritance, involving a single gene, DFNB1 which codes for connexins which are expressed in the cochlear. [97] [98]
Symptoms: Prelingual, bilateral, prevalently stable sensorineural defect ranging in severity from mild to profound, depending on the amino acid sequence of the mutation. As a result this can lead to profound deficits on speech and language development. In general, the hearing impairment does not progress. Cochlear implants are a common treatment option to treat patients with connexin mutation related hearing impairments. [99] [100]
Skin Disorders
Types: Erythrokeratodermia variabilis (EKV), palmoplantar keratoderma (PPK), Vohwinkel syndrome (VS) and hidrotic ectodermal dysplasia (HED)
Connexin Mutations: Cx26, 30, 31
Description: Cx26 in the epidermis plays an essential role in coordinating keratinocyte growth and differentiation. EKV: Autosomal dominant disease. Environmental factors trigger the formation of migratory red patches that regress and reappear. The disease is caused by a mutation on the 1p34-35 chromosome which alters the voltage-gating and pore formation of the gap junction. PPK: Involves a mutation on the GJB2 gene, which tends to lead to clinical manifestations involving the palms and soles. VS: a specific form of PPK, is caused by a mutation of the D66H allele of the Cx26 connexin. HED: autosomal dominant disease, caused by variations in the G11R and A88V alleles of the GJB6 gene which encodes Cx30. It is thought that this changes the voltage-gating properties of gap junctions. [101]
Symptoms: Generalized brown hyperkeratosis with sharply demarcated hyperkeratotic plaques most often on the neck, extensor surfaces of the arms and legs and the buttocks. Hyperkeratosis of the palms and soles, often associated with peeling. Band-like constriction of fingers that lead to autoamputation, alopecia and nail abnormalities. [102]
Cardiac Dysfunction
Connexin Mutations: Cx43, 40, 45
Description: Connexin expression in diseased human tissue is as a result of either structural remodeling, alteration in the distribution and organization of gap junctions or remodeling of the connexin expression, including changes in the amount and type of connexin present. This is thought to contribute to abnormal conduction and arrhythmogenesis in diseased hearts. Mutations also lead to ventricular hypertrophy and pulmonary artery stenosis. Laterality defects occur due to Cx43 mutations. The number of connexins per intercalated disks can also be reduced. [103]
Symptoms: less coordinated spontaneous beating, myocardial infarction, first-degree atrioventricular block with associated bundle branch block, hypoplastic left heart, atrioventral canal defects, hypertrophic cardiomyopathy and ischeamic heart disease. [104]
Dominant Zonular Pulverulent Cataract
Connexin Mutations: Cx 46, 50
Description: An inherited, autosomal dominant disease caused by a missense mutation results in a substitution thought to be at codon 63 of Cx46. Mutations in the connexins cause a disruption in the homeostasis of oxygenation and hydration in the lens of the eye. Normal transparency of the lens is lost, leading to lens opacification. [105]
Symptoms: Lens opacities that have pulverized, dust-like appearance of the lens. Numerous powdery or punctuate opacities located in different developmental regions are evident. [106]
Oculodentodigital Dysplasia (ODDD)
Connexin Mutation: Cx43
Description: An autosomal dominant syndrome associated with a mutation with the connexin Cx43, or GJA1 gene, located on chromosome 6q22-q23. The mutations can cause misassembly of channels or alter the gap junction channel conduction properties. This leads to craniofacial and limb dysmorphism and neurodegeneration.
Symptoms: Typical craniofacial abnormalities include a thin nose with small anterverted nares, brittle nails and hair which are slow growing, ophthalmic abnormalities including microcornea, glaucoma, optic atrophy. Mandibular overgrowth, cleft palate, teeth abnormalities such as enamel hypoplasia and early tooth loss are also common. Hand and foot abnormalities most often involve syndactyly of the fourth and fifth finger and the second to fourth toes. Neurological symptoms are frequent and include dysarthria, ataxia and spastic paraparesis. [107]
Charcot-Marie-Tooth Disease
Connexin Mutation: Cx32
Description: Hereditary heterogeneous disease which affects the motor and sensory nerves of the peripheral nervous system. Transmission of the disease caused by a connexin mutation is linked X-linked, being on the X chromosome. Myelin sheaths and/or axons of the peripheral nerves are affected through degeneration or abnormal development. This leads to a decrease in the efficiency and velocity of nerve conduction. The Cx32 mutations are missense, nonsense, frameshift or deletion. [108] [109]
Symptoms: Muscular atrophy of the 4 extremities, particularly foot deformities such as 'cavus foot', abnormal or altered gait (mostly have a high step), muscle weakness and wasting and sensory loss, frequently depressed tendon reflexes and abnormal electrophysiological testing. [110] [111]
Current Research
The main areas of current and future research regarding gap junctions are their involvement in disease. The research is aimed at understanding from the simplest individual gap junction proteins to the whole organism.
Heterotypic Gap Junctions between Two Neurons in the Drosophila Brain Are Critical for Memory. (2011)
Recent studies have identified a link between gap junctions and memory but the mechanism which it does this has not been clarified. This study aims to discover the process by which gap junctions contribute to memory function. RNA interferance-mediated knockdowns of specific regions in the anterior paired later and the dorsal paired medial neurone of Drosophila fly brains were analysed. These two neurones form gap junctions in a part of the brain that is responsible for learning and memory. Results show that with modifications to the Drosophila's RNA, the organisms' anesthesia-sensitive memory is dramatically impacted. A direct link between the anterior paired later and dorsal paired medial neurones can be established with memory formation. [112]
The clinical features of patients with the homozygous 235delC and the compound-heterozygous Y136X/G45E of the GJB2 mutations (Connexin 26) in cochlear implant recipients (2011)
Hayashi et al. aimed to investigate the proportion of patients with cochlear implants who had a 235 deletion C mutation on the GJB2 gene. The GJB2 gene codes for connxein 26, which is strongly associated with hearing. This mutation was found in 14.4% of the alleles, in different variations. All of the subjects had severe hearing loss, some of which had progressive hearing loss. All patients recorded better performance after having the cochlear implantation. Some complications were found in 4 alleles (2 patients). Poorer outcomes were suggested to be linked to brain function and unexpected complications. [113]
Regulation of Connexin 43 by Basic Fibroblast Growth Factor in the Bladder: Transcriptional and Behavioral Implications (2011)
The gap junction protein, connexin 43, is up-regulated in the overactivity of an obstructive bladder. Although there are numerous steps involved in the pathway from DNA to protein expression in individual tissue, transcriptional control is one of the most important steps in Cx43 protein regulation. Western blotting was used to analyse extracellular regulated kinases as well as connexin 43 in bladder proteins. Results lead to beleive that extracellular signal regulated kinase protein as well as connexin 43 could be potential theraputic targets for increased urinary frequency.[114]
Connexin 43 gene expression in male and female gonads of porcine offspring following in utero exposure to an anti-androgen, flutamide (2009)
Flutamide are anti-androgens, compounds which are capable of inhibiting the effects of androgens (male sex hormones). This study aims to investigate the effects of maternal exposure to flutamide on the expression of connexin 43 in testes and ovaries, particularly in piglets. Immunochemistry, Western Blotting and RT-PCR were the techniques used to perform this study. From the evidence shown, there were no obvious changes in either the testes or ovarian morphology with connexin 43 being present in all the tissues examined. Androgens were therefore not believed to be involved int eh control of connexin 43 in the genetic expression of gonads in piglets. [115]
Amacrine Cells: Interneurons in the retina
Arrhythmogenesis: Conditions in which there is abnormal electrical activity in the heart.
Autoamputation: A spontaneous detachment from the body.
Autosome: A chromosome that is not a sex chromosome.
Connexin: Gap junction proteins, structurally-related transmembrane proteins that assemble to form vertebrate gap junctions
Connexon: An assembly of six proteins (connexins) that form gap junctions.
Dysmorphism: Any malformation which is suggestive of a congenital disorder, genetic syndrome, or birth defect.
Electrophysiological: Electrical results produced through physiological work, or by change of action in a living organism.
Folliculogenesis: Maturation of the ovarian follicle
Heteromeric: Multiple types of connexion
Heterotypic: Adjacent cells consisting of differently composed connexons
Homomeric: A single type of connexin
Homotypic: Adjacent cells consisting of identically composed connexons
Intercalated Disc:: Transversely oriented bands throughout cardiac muscle
Laterality: Localization of greater function in either the right or left side of the body.
Neurotransmitter: Chemicals which allow the transmission of signals from one neuron to another neuron
Opacification: The process of becoming opaque/cloudy.
Syndactyly: A condition where 2 or more of the digits are fused together (partially or totally).
- ↑ 1.0 1.1 Alberts B, Johnson A, Lewis J, et al. 2002 Molecular Biology of the Cell, 4th edn, Garland Science, New York
- ↑ Joell L. Solan & Paul D. Lampe, 2005, 'Connexin phosphorylation as a regulatory event linked to gap junction channel assembly', Biochimica et Biophysica Acta (BBA)- Biomembranes, vol. 1711, no. 2, pp. 154-163.
- ↑ Dale W. Laird, 2005, 'Connexin phosphorylation as a regulatory event linked to gap junction internalization and degradation', Biochimica et Biophysica Acta (BBA)- Biomembranes, vol. 1711, no. 2, pp. 172-182.
- ↑ 4.0 4.1 4.2 4.3 Simon A.M.,Goodenough D.A., 1998, 'Diverse functions of vertebrate gap junctions', Trends in cell biology, vol. 8, no. 12, pp. 477-483.
- ↑ Peracchia C., 1977, 'Gap junction structure and function', Trends in Biochemical Sciences, vol. 2, no. 2, pp. 26-31.
- ↑ <pubmed> 15940850</pubmed>
- ↑ <pubmed> 17770946</pubmed>
- ↑ <pubmed> 14206423</pubmed>
- ↑ <pubmed> 14069795</pubmed>
- ↑ <pubmed> 14206423</pubmed>
- ↑ <pubmed> 6036535</pubmed>
- ↑ <pubmed> 8534895</pubmed>
- ↑ <pubmed> 5531667</pubmed>
- ↑ <pubmed> 4105112</pubmed>
- ↑ <pubmed> 4339819</pubmed>
- ↑ <pubmed> 4363961</pubmed>
- ↑ <pubmed> 885916</pubmed>
- ↑ <pubmed> 889612</pubmed>
- ↑ <pubmed>312530</pubmed>
- ↑ <pubmed>8534895</pubmed>
- ↑ <pubmed>11894941 </pubmed>
- ↑ <pubmed>2408491</pubmed>
- ↑ <pubmed>8534895</pubmed>
- ↑ <pubmed>2875078 </pubmed>
- ↑ <pubmed>2987225</pubmed>
- ↑ <pubmed>2557354</pubmed>
- ↑ <pubmed>8665925</pubmed>
- ↑ <pubmed>1655801</pubmed>
- ↑ <pubmed>8665925</pubmed>
- ↑ <pubmed>7892609</pubmed>
- ↑ <pubmed>12108537</pubmed>
- ↑ <pubmed>9139825</pubmed>
- ↑ <pubmed>10024245</pubmed>
- ↑ <pubmed>12108537</pubmed>
- ↑ <pubmed>15109565</pubmed>
- ↑ <pubmed>16037090</pubmed>
- ↑ <pubmed>16707726</pubmed>
- ↑ <pubmed>18946008</pubmed>
- ↑ [1] Pubmed Gap Junction Search
- ↑ 20542681
- ↑ 885916
- ↑ 17934503
- ↑ 20960023
- ↑ 15109565
- ↑ 20066080
- ↑ 8665925
- ↑ 17934503
- ↑ 20066080
- ↑ 20542681
- ↑ 9184217
- ↑ 20066080
- ↑ 17004931
- ↑ 20234156
- ↑ 8188753
- ↑ 20234156
- ↑ Vinken M, Decrock E, De Vuyst E, Ponsaerts R, D'hondt C, Bultynck G, Ceelen L, Vanhaecke T, Leybaert L, Rogiers V, 'Connexins: sensors and regulators of cell cycling', Biochim. Biophys. Acta: 2011, 1815(1); 13-25
- ↑ Franchesca D Houghton, 'Role of gap junctions during early embryo development',Reproduction: 2005, 129(2); 129-35
- ↑ David L. Paul, 1995, 'New functions in gap junctions', Current Opinion in Cell Biology, vol. 7, pp. 665-672.
- ↑ Alberts B, Johnson A, Lewis J, et al. 2002 Molecular Biology of the Cell, 4th edn, Garland Science, New York
- ↑ Gerald M Kidder, Abdul Amir Mhawi, 'Gap junctions and ovarian folliculogenesis' Reproduction: 2002, 123(5); 613-20
- ↑ 20234156
- ↑ 19943035
- ↑ 19369543
- ↑ 10339616
- ↑ 19176884
- ↑ 12006089
- ↑ 19116647
- ↑ 11150340
- ↑ 18415116
- ↑ 15151915
- ↑ 18415116
- ↑ 18415116
- ↑ 19632896
- ↑ 21179475
- ↑ 18162609
- ↑ 21179475
- ↑ 18565824
- ↑ 21179475
- ↑ 18386043
- ↑ 20066089
- ↑ 17934502
- ↑ 20066089
- ↑ 20467217
- ↑ 1883873
- ↑ 10201522
- ↑ 10201522
- ↑ 1883873
- ↑ 19736524
- ↑ 10201522
- ↑ 20215342
- ↑ 20215342
- ↑ 10414969
- ↑ 20041195
- ↑ 20130418
- ↑ 10616683
- ↑ 16340443
- ↑ 10767631
- ↑ 17222463
- ↑ 15079193
- ↑ 15656949
- ↑ 11122011
- ↑ 12923422
- ↑ 15033584
- ↑ 20140684
- ↑ 12923422
- ↑ 10205266
- ↑ 12457340
- ↑ 19825739
- ↑ 21504497
- ↑ 12207932
- ↑ 10767631
- ↑ 21530256
- ↑ 21292415
- ↑ 21511298
- ↑ 19853283
|2011 Projects: Synaptic Junctions | Gap Junctions | Tight Junctions | Desmosomes | Adherens Junctions | Neuromuscular Junction


text:
New DWTS Season 12 Cast Rumors – Wendy Williams, Candice Cameron Bure, and Carlos Leon Possibilities & More
Finally, some serious Dancing With The Stars Cast rumors coming out. WTMA via The New York Post is reporting TV talk show host Wendy Williams will be a contestant on Season 12 of Dancing with the Stars. The quote below also mentions Kendra Wilkinson, Candice Cameron Bure, and Lorenzo Lamas (Lorenzo and Kendra we've heard might do the show before).
The New York Post reports Williams will put her show on hiatus while she participates next month. A spokesperson for The Wendy Williams Show was unable to confirm the report.
Other celebrities rumored to be joining the cast this season include reality star Kendra Wilkinson of Girls Next Door, former Full House star Candice Cameron Bure and former Renegade star Lorenzo Lamas.
Here is a picture of Wendy Williams in case you don't know who she is.
Here is a picture of Candice Cameron Bure.
This is a "wannabe" long shot. But, Marc Malkin of E Online (who is known for sticking some DWTS rumors out there that end up being true which is why I thought this might be worthy of a mention?) is reporting Carlos Leon (a former flame of Madonna's and father to her daughter Lourdes) has recently expressed he wants to do the show. Madonna first met Carlos in a gym. They've worked out together and have been friends ever since.
One of her ex-boyfriends tells me he wants the chance to compete for the mirror ball trophy.
And it's not just any ex…
"I"ve gotten quite a few offers to do reality shows," says Carlos Leon, AKA Lourdes' dad. "I got offered to do Dancing With the Stars a couple years back. I didn't do it, but if they come up with the offer again, I would totally do it."
Here is Carlos.
So, talk to us. What do you think of these possibilities? I'm open to all the above. If Candice is dancing, I hope she gets Maks and I'd love it if Carlos danced on the show. I bet he has some moves. I can see him with Cheryl. I bet they could pull off a killer Argentine Tango!
Thanks to PureDWTS readers Nathan and Vin for the help with this post! Heidi too for adding some links from past posts on Lorenzo and Kendra.
ETA: And Candace says NO.
[blackbirdpie url="http://twitter.com/#!/candacecbure/status/37985383207804929″]


text:
PawSox homers sink Bisons Sunday
Josh Satin's first Triple-A home run pushed the Bisons ahead in the eighth, but Lars Anderson responded with his own two-run homer, taking a 7-6 Pawtucket win Sunday afternoon at Coca-Cola Field.
Satin had a chance for a walk-off, but center-fielder Che-Hsuan Lin made a running catch to end the game and strand two runners.
"I hit it well, barely off the end of the bat," Satin said. "It's just a learning process for me. I wish I could've come through for the team, but it's definitely a step in the right direction.
In a save opportunity Dale Thayer pitched allowed a run on a Daniel Nava sacrifice fly and walked Ryan Lavarnway before the game-winning blast.
Satin's homer against Hideki Okajima scored Jason Botts, who had walked, in a 4-4 tie.
"At that point I just wanted to get a hit to get the rally started," Satin said. "To hit a home run was definitely something that I wasn't thinking about but it was great for me personally and, at the time, for the team."
Tony Thomas blasted his third homer in Buffalo and eighth of the season, giving the PawSox a 2-0 second-inning lead.
After a single and a stolen base, Fernando Perez raced home and Luis Figueroa moved from first to third on a fielding error by first baseman Anderson off the bat of Jesus Feliciano.
On the next pitch, Valentino Pascucci brought both runners home on a broken-bat double to right.
In the fourth, Luis Exposito tied the game at three with a leadoff homer off the left-field foul pole.
Starter Dylan Owen allowed four earned runs on five hits over 6 2/3 innings, taking a no decision.
"Owen did a great job," manager Tim Teufel said. "It was just the bullpen at the end. (It) let things get away from us a little bit."
With the game still tied at four, Thomas nearly took back the lead in the top of the eighth, but his long center-field hit with Nate Spears on first bounced out of play, holding the runner at third for a ground rule double.
Game Notes: Thomas hit grand slams during the teams' first series of the season on April 9th and 11th… Pascucci leads the Herd with 68 RBIs… Satin hit 11 home runs in Double-A Binghamton, where he started the season… Pawtucket leads the season series, 9-2, winning the last few seven.


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Mets look to Takahashi
My guess is that Ken Takahashi didn't need a translator to understand some variation of "You're going to the Big Leagues" or "Welcome to the Mets."
Mets.com is reporting the veteran reliever was added to the 40-man and the big leagues rosters an hour after the Mets' 8-1 loss to the Nationals and after the club designated left-handed reliever Casey Fossum for assignment.
READ: Takahashi story on Mets site
Takahashi has appeared in six games with the Mets' Triple-A Buffalo affiliate, producing a 0.77 ERA in 11 2/3 innings. He allowed 10 hits and six walks and struck out nine.
Fossum had appeared in three games with the Mets, including their loss on Sunday.
~ Ben Wagner
Some content provided by Mets.com


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In an effort to protect your business identity and information, our office provides
a free, easy-to-use Business Filing Notification System that allows businesses to
track any changes and updates to business filings with our office.
In order to subscribe for the Business Filing Notification System or unsubscribe
for existing notifications(s), business or individuals will need an e-mail address
and business charter, registration or license number for each filing, which can
be found through an online business search. Click here to search the Secretary of State's Business Entity Database.


text:
Cara Beach Brazilian
€ 60,00 € 39,00
Made of recycled yarn
Made with more than just the beach in mind.
The comfortable, soft material reminds of the surfer look.
Quick dry / Flat, bonded seams at the leg, waist and sides
Order Today: Estimated Delivery Date 06/10/2020
- main material 74% polyamide, 26% elastane


text:
Peer-Reviewed Publications
Found 20 results[ Title]
Filters: Keyword is Cross-Sectional Studies and Author is Flaura K Winston [Clear All Filters]
Acute healthcare utilization by children after motor vehicle crashes." Accid Anal Prev. 2004;36(4):507-11."
Assessing child restraint misuse by parental survey." Inj. Prev.. 2000;6(2):145-7."
Belt-positioning booster seats and reduction in risk of injury among children in vehicle crashes." JAMA. 2003;289(21):2835-40."
Effects of seating position and appropriate restraint use on the risk of injury to children in motor vehicle crashes." Pediatrics. 2005;115(3):e305-9."
An evaluation of the effectiveness of forward facing child restraint systems." Accid Anal Prev. 2004;36(4):585-9."
Exposure to traffic among urban children injured as pedestrians." Inj. Prev.. 2002;8(3):231-5."
Factors associated with front row seating of children in motor vehicle crashes." Epidemiology. 2004;15(3):345-9."
Factors influencing pediatric injury in side impact collisions." Annu Proc Assoc Adv Automot Med. 2000;44:407-28."
Grandparents driving grandchildren: an evaluation of child passenger safety and injuries." Pediatrics. 2011;128(2):289-95."
Hidden spears: handlebars as injury hazards to children." Pediatrics. 1998;102(3 Pt 1):596-601."
Optimal restraint reduces the risk of abdominal injury in children involved in motor vehicle crashes." Ann. Surg.. 2004;239(1):127-31."
Parent driver characteristics associated with sub-optimal restraint of child passengers." Traffic Inj Prev. 2006;7(4):373-80."
Primary access to vehicles increases risky teen driving behaviors and crashes: national perspective." Pediatrics. 2009;124(4):1069-75."
Recent trends in child restraint practices in the United States." Pediatrics. 2004;113(5):e458-64."
Risk of injury to child passengers in compact extended-cab pickup trucks." JAMA. 2002;287(9):1147-52."
Seat belt use among 13-15 year olds in primary and secondary enforcement law states." Accid Anal Prev. 2007;39(3):524-9."
Teen drivers and the risk of injury to child passengers in motor vehicle crashes." Inj. Prev.. 2005;11(1):12-7."
Trip characteristics of vehicle crashes involving child passengers." Inj. Prev.. 2005;11(4):219-24."
Variation in teen driver education by state requirements and sociodemographics." Pediatrics. 2012;129(3):453-7."


text:
The automatic out is back in the lineup as SS tonight. Good to see Heisey back in CF. I thought Frazier would have had tonight off.
1. Brock Holt (L) 2B
2. Alex Presley (L) RF
3. Andrew McCutchen (R) CF
4. Garrett Jones (L) 1B
5. Pedro Alvarez (L) 3B
6. Jose Tabata (R) LF
7. Clint Barmes (R) SS
8. Rod Barajas (R) C
9. Wandy Rodriguez (S) P
1. Brandon Phillips (R) 2B
2. Chris Heisey (R) CF
3. Joey Votto (L) 1B
4. Ryan Ludwick (R) LF
5. Jay Bruce (L) RF
6. Todd Frazier (R) 3B
7. Dioner Navarro (S) C
8. Wilson Valdez (R) SS
9. Mat Latos (R) P


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Thursday, October 04, 2012
A Garden That's Beautiful and Accessible
Check out this article in the San Francisco Chronicle by Yvonne Michie Horn on HolLyn D'Lil's garden in Graton, California. This garden would be intriguing just for its labyrinthine paths, butterfly chairs, and creative use of salvage alone. But the fact that it is designed to be wheelchair-accessible makes it even more remarkable. D'Lil planned the garden with themes and designs "honoring the body, mind, and heart" and she can tend it all from her wheelchair using long-handled tools. So inspiring!
Subscribe to: Post Comments (Atom)


text:
37.50 m Motorsailor traditionally built -Iroko on mahogany - ready for inspection here in Southern Turkey... ask for more details...
Operating nearly for 30 years and a long time member of TURSAB, ASTA and UFTAA; Gino Group is well established company with the worldwide reputation as a leading yacht agency in Turkey. We are based in Marmaris/Netsel Marina, the heart of yachting tourism in Turkey and we have branches in Istanbul, Kuºadası, Bodrum, Göcek, Marmaris-YalancıboÄŸaz and a representation office in Antalya and the dealer of BENETTI Yachts and BAIA Yachts Turkiye. Our target is always for excellence and we are proud of the fact that GINO GROUP today is one of the largest and most influential companies in the Turkish Yachting Industry.
Netsel Marina


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Salt and pepper the ribs and sprinkle them with flour.
In a large Dutch oven or heavy covered pot, brown the ribs in 5 tablespoons of the vegetable oil and set them aside.
Wipe the pot clean, add the butter and remaining 2 tablespoons of oil, and add the garlic and onion. Cook for about 2 minutes.
Add the tomato paste, broth, bay leaf, thyme, and the allspice. Stir well, then add the mushrooms and veal ribs.
Cook, covered, over low heat for 1 1/2 to 2 hours. If the sauce becomes too thick, add more broth. The bay leaf should be removed after 30 minutes of cooking. Sprinkle the parsley on top just before serving.
2871 Jolly Road
Okemos MI 48864
tel 855.328.8732
fax 517.253.7366
Contact Us
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You can only have one driving region on your profile, but you may be able to drive in different states.
Existing drivers can choose a city or state at State & City Driver Information to see specific in-state license, insurance, and registration requirements. New drivers can log in to Driver application requirements to see driver, document, and vehicle requirements for their city.
If you're making a permanent switch, let us know. When you're ready to switch to a different Lyft community, send us the following:
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Tap 'Contact Support' below to send us your info.


text:
ATLANTA, GA – The Clemson diving team posted some solid scores in the diving portion of the Georgia Tech Invitational, which took place over the weekend and concluded on Sunday.
Among the leaders in the one-meter was Juliana Melchionda, who finished in 12th place in qualifying with a score of 242.15 and made the cut for the finals. Tabitha Banks (212.85) and Jessica Resler (205.40) also finished in the top 15 in the event.
Melchionda also finished 12th in the preliminary round in the three-meter with her score of 274.60. Resler placed 14th with a score of 228.75, and Banks and Emily Yoder both placed in the top 20 in the event.
In the platform, Resler was the Tigers' top performer, finishing seventh with a score of 197.35. Yoder also placed ninth in the event with a score of 182.10.
The Tigers will rejoin the rest of their teammates that competed in the University of Missouri Invitational after a month off for the University of Tennessee Invitational on January 3.
September 23, 2020
September 22, 2020


text:
Topic: Pole boys
we are a group of kids who fight the nazis through guerilla tactics so make a charactor sheet and show skill and start postin
Message Boards - Boba Fett Fan Club → Role Playing → Pole boys
this rpg take place in nazi occupied poland
we are a group of kids who fight the nazis through guerilla tactics so make a charactor sheet and show skill and start postin
name:mike Checlovov
age: 17
skills: good sniper and leader of pole boys
history: he was 14 when nazis invaded he has fought them since he was 15
weapons: a german mp40 and 98k infantry rifle
Name: Zank Offiuon
Age: 13
Power: 20
Skills: 19/50
Weapon: Grenade, pistol.
history: he was found, lying in a smoking house that the Nazis set fire to.
Nationality: Scottish
Zank looked around. It was everywhere! the Nazis camp was only 18 metres away from where he was standing.
Bullets whirred and screams echoed the sky. He stuck close to his friend, Mike Checlovov, a 17 year old spy of the Kid System Group. Also known as the KSG.
Zank reloaded his 0.53ZX german pistol and they set off. The other 10 members of the group would come soon.
He stopped a German. " hide! " he whispered in Mike's ear. The German cursed in German and spat. he loaded his rifle and looked around for intruders.
Zank shot the German right in the chest. He cursed and looked round. He raised his rifle at the two boys. Zank shot him again, this time in the shoulder. The german shrieked and fell over.
This was going to be hard, thought Zank, as they trudged on to the base.
Name: Edward Florian
Age: 14
Weapon: Whiskey Bomb, Home made sticky bomb, stick grenade,
M1942 Beretta SMG, "Bouncing Betty" Mines, and Tellermine.
Skills: Demo, and weapon, and infiltration specialists and stratigest.
History: Parent were kill when he was five {theres more but I have to go}
Zank and Mike were followed closely then by their other friend member, Edward.
He carried a stick grenade. He nodded to Zank and Mike and threw it. There were three guards out on patrol beside the gate. The grenade exploded and the German Patrol's fell over dead.
Edward then unfurled his M1942 Beretta SMG. That was his 2nd favourite weapon.
Zank got out his map of the German base and pointed the next direction of which to take.
Damn! Thought, Zank. There were were six Germans who had spotted the team and they shouted at them and unfurled their Machine-Guns..............
Name:Chad Hill
Weapon(s):Karabiner 98k,M1911 pistol,MP5,AK-47,Flash Grenade,Grenade
Skills:35/60 Good with computer
Chad ran into German base,there he picked up his Karabiner 98k and shot two german guards.German guards spotted him and ran after him.
Chad ran,ran and ran.After 15 minutes he spotted Zank,Mike and Edward...
Zank then saw a burst of machine gun fire. Two of the German Patrol went down. The four remaining turned round and sped after a boy......
A boy............ That was part of Edwards's KSG. It was Chad Hill! A large teenage boy that was the most Trustworthy and careful. He was aware too.......
Zank cursed and beckoned to Edward.
" Come on! " He shouted. " Chad needs our help! We can't just let him die for Two ( censored ) German's!! "
" Move out! "
He unfurled his German revolver and shot at a German. He went down, smoke shooting out of his Back.
Three left. Zank thought.
Would they be able to save Chad in time from The Patrol?
Skills:35/60 Good with computer
except for there were no computers back during world war two....
( I think he means " good with machinery and engineering , AA. Give him a chance. He is Finnish you know. )
Name: Edward Florian
Age: 14
Weapon: Whiskey Bomb, Home made sticky bomb, stick grenade,
M1942 Beretta SMG, "Bouncing Betty" Mines, and Tellermine.
Skills: Demo, and weapon, and infiltration specialists and stratigest. and suprot men
History: Parent were kill when he was five {theres more but I have to go}
He lived on the streets till the invasion of Poland where he has used his knowledge of the back streets and ally against the german war machine.
The AK-47 was not invented in the world war 2, trust me I know a lot about ww2, or flash grenades.
{hey can we have two characters?}
"Grenade going in!" said Edward.
Boom 2 went down.
Edward quickly mowed down the other one.
"I'll bet the hole set up knows we are here!" Whispard Edward. "I told you to take the other way," said Edward.
"god damnit edward i already know that" sadi mike as he shot a german with his 98k
then he swithced to his mp4o to give the group covering fire
"move damnit, now Zank i said move it!!"
they all ran for the trees as mike got to the place where the det for the tnt was he slamed the lever was and watched with pide as the whole base blew
"good job guys all of u i mean it"
karson yea what the hell why not i guess u can have two
but i was thinking we need a girl to be like a spy
"We got the depot to deal with next," Said Edward. "What?" asked Mike. "I we got to help the germans out some how," said Zack sarcastically. Mike looked completely surprised. "He means we need to cripple the tank's supply of fuel," said Edward.
{Any body that wants to know about the weapons and what weapons where used in World War 2 http://www.bayonetstrength.150m.com/Tac … ar_tw.htm}
{Ha I am watching a program about World War 2, when the Nazi forces invaded Poland!}
"good idea Ed, if we take out the fuel supplies then we should be able to 'borrow' a car from the germans" said mike
they alll started running for the fuel depot to demo it when all of a suden it blew up
"down everyone"said Ed as he tackled mike
"thanks ed"
"no problem mike, hard for u to shoot Krauts if your dead'
good point he thought
"ok guys time to get a ride, ed any ideas? i was thinking of a halftrack"
"yea that sounds good"
they all got on an abandoned half track and mike got in the drivers seat turned it on and they started back to what the called the pole boys hq...
it was just a old cabin in the woods acssesable only by foot
then they started the long process of figuring out what in the hell they destroyed...
Name: Ted Bridge
Age: 13
Weapons: Karabiner 98k, M1911 pistol, grenades
Skills: 20/50, good with stealing and spying
History: Nazis took him from England and bring him to poland. Escaped from them and joined KSG.
Pole boys were just starting make a plan, when they heard a tank noise coming out. They all run out. The tank stopped but didn't shoot them. Then Ted Bridge came out from. "Look what I stealed from Nazis!!" he shouted. "Holy, Ted!!" other Pole boys answered.
As the boy neared the cabin, Edward saw the sight he saw all to often; a cabin armed to the teeth with Anti tank weapon that the boys had Borrowed from the Germans, Some MG 34s and 42s, witch the boys had also borrowed from the Germans. And about 10000 meters from the cabin booby traps, A.T. mines, and spike strips. The cabin was fortified with sand bags, large rocks and dikes (large, long mounds of dirt) . Only the Bots knew where the Mines and traps where.
The cabin was a ammo, food, shelter house for the boys. The cabin had been reinforced from the inside with
steel and large rocks. Making the once peaceful cabin, and woods, a treacherous place to tred and a oh, so safe bomb shelter .
"Ahh, it so good to be home!"said Zack.
"It is,"Said Mike
"Aye,"said Edward
Zank rested himself in the abandoned camp and flopped on the bunk. He started reading his novel and sipping an orange squash that was left by the last KSG that went missing three months ago.
Zank reloaded his smuggled German assault rifle. He fitted 25 rounds into it and got up to slot four anti-tank mines outside the base. He placed them 20 metres away so if a tank did come out , the base would'nt explode with them inside. He flopped on the bunk and continued to read his novel..............
Edward, after he rested a little, walked back to the blow German base to salvage what he could from the base.
Edward returned after 30 minutes with 5 boxes of ammo for pistols and Assault rifle's and ordinary rifle's, two bags of fresh fruit, meat, potatoes and vegetables and some german armour. It was too big for them, but Ted could mow it down and turn it into the right sizes for all of the KSG........................
-F3TT- wrote:
Skills:35/60 Good with computer
except for there were no computers back during world war two....
Sorry.LOLZ. Then Good with engineering as Green said.
I like it when you use the colour " Green " for my name.
It's Class!
"so guys i was thinking we try a different way tobring down the krauts"said mike
"what"said ed
"well i was thinking we try to infiltrate the germans here"
"what"said zank as orange juice went everywhere
"well it might work only two or three would go"
Zank spilled his Orange Juice everywhere!
" What?! " Zank said, upright, choking on the orange liquid in his throat.....
" Well it might work if only two or three would go...."
" Okay, then, I'll go. I'll take Edward and Chad with me." He said, gripping them tightly round the arms and shoving them outside.
" What the hell--? " Chad muttered, who was half-asleep, due to lack of strength and courage.
" Hmmmmmm! I'm not sure.....the German's could be armed with boms and missiles...." Edward stated.
" Come on! " Zank shouted. " Do you want to spend the rest of your life inside a stinking shelter that drips soil and grass everywhere!!?? "
" Ummmm, not exactly, no. " edward replied.
" Well, then let's go! I'll pack us food , water and ammo... "
" but their armed with missiles! " edward whined. " We only have guns, armour and grenades! We-- "
He stopped whining as Zank pushed him and Chad out of the shelter............
" Move out! " Zank ordered. Then they went towards the german base................
Edward returned after 30 minutes with 5 boxes of ammo for pistols and Assault rifle's and ordinary rifle's, two bags of fresh fruit, meat, potatoes and vegetables and some german armour. It was too big for them, but Ted could mow it down and turn it into the right sizes for all of the KSG........................
(Germans had no armor!)
This is 1944 for petes sake!
Sheesh! I don't know if they did!
I'm not such a nerd of WWI and WWII.
Please don't crap at me.
( It means getting on at if ya don't know )
guys i menat that me would infiltrate their ranks like spys
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text:
Chemical tailor's chalk, abt. 40 x 40 mm, suitable for all textiles, evaporates immediately when ironing, in boxes of each 50 pcs., weight per box abt. 0,450 kg, available only in nature white.
Large technical drawing triangle, plexiglass transparent, removable handles, angle graduation 180° - 1°, recurrent and coloured angle number figuration with ink-edge, hypotenuse 325 mm.
Pin-cushion, with plastic bracelet.
Chalk pencil, wooden shaft 175 mm long, 8 mm Ø, to mark buttons, button holes, and other things, in packages of each 12 pcs.
Cleaning Pads, of fine steel wool, for removing residues from iron soles or plates.


text:
Posts Tagged promotional advertising
You Want A Salesman, I'm Not Your Guy
I decided to write this without pausing. Just what comes to my mind and let my fingers hit the keys. Business thoughts.
I didn't get into business to be like everyone else.
Always told it couldn't be done this way. Always told it is about the money and can't make money this way.
I hate the image of a salesperson. So why did I get into sales in my own business? I don't have to do what others tell me to do.
Sure I want to make money. Sure I want a make a nice living. But I want to help others along the way. I want others to be ok with me making money because my efforts deserve it. Because I am not a slick order taker. Order takers deserve bupkus! A computer screen and website can take an order.
I want to learn about your business. I want to be considered part of your marketing team. Develop ideas and present to you. Pen orders are for computers. Oh, I will place them for you and make sure you get a great price. But, you don't really need me. I don't have much value.
I located a tribe. This tribe wants to do something different. The leader calls himself the Creative Director. His name is Kamran Popkin We connected on twitter. He calls his tribe the Swag Club. Half the time I don't understand what he is saying. He has his own language of sorts. But, I respect him. His outlook and what he is trying to do for the promotional advertising (or swag) business.
Behind the swagger, lives a man of heart. A family man. Along with a beagle that gets him out of bed way too early! We have shared books. He even sent me one in the mail.
I barely knew him and he shipped a book of stories on Faith along with some other goodies. Wow.
Again, I barely knew him. I had nothing that could really benefit him. Giving without expecting in return. The tribe. I liked it and him already. Even if I got lost in his dialogue.
I am not officially part of his "tribe", but he nudges me. He offers support. Keeping steady and strong in a save me a few pennies, ordertaking world . Especially when balances scream.
Catalogs – forget it. Koozies — blasphemy. He shares my desire to be the bright red flower in the bed of overgrown weeds of order takers.
My idea – Get excited. Fresh ideas. Let me inside your goals. Then let me bring you some thing that explodes.
Hate my ideas–hang up. Not interested in what I show you….show me the door. You don't owe me a penny.
But if I surprise you. If I come up with an idea that gives you some chills. Bingo—we both win.
Back to my original question. Why sales if I hate salespeople?
You missed the point.
I don't want to sell. I want you to dig my ideas. I want you to ask me to sit and talk and explore. After all, unlike an attorney, I don't charge by the minute. Only for ideas. Sounds like fun huh?
This was free flow. Ideas coming out fresh and amazing how fast. Few minutes.
Only thing I ask, please forgive grammar or sentence structure errors. I am sure there are many.
Throw out a comment below: Am I out of my mind? Do I have a chance to create something or fools gold? Was writing like this a good idea or ……not so good?


text:
Straight from the twitter account of the man himself, the NIN iPhone app has been approved and will be available sometime later today "unchanged" from the version that Apple found objectionable.
So, it appears that Apple has caved in to the criticism, further illustrating that their approval system is all kinds of messed up. All that aside, I'm just happy it's on it's way. [Twitter]


text:
A young woman holds the poster of a Palestinian prisoner on hunger strike at a demonstration in Ramallah on Thursday.
Relatives of Palestinians in Israeli prisons gather daily at a solidarity tents set up in every city.
Banners in clock square, where the Ramallah demonstration ended on Thursday.
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