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Type01 Chest of Drawers
- Taxes included
- Free delivery Free deliveryEnjoy free professional delivery – right to your doorstep. Your shelf will arrive in labelled flat pack boxes for quick, easy assembly. We ship to all EU countries, including Switzerland and Norway.
- 100 days to fall in love 100 days to fall in loveIf for any reason you're not happy with your shelf, we'll pick it back up for free within 100 days and give you a full refund. No questions asked.
The Type01 Chest of Drawers
Meet our top-drawer storage
At home in any space, this chest of drawers offers cleverly concealed storage where you need it most. Enjoy hassle-free shopping, consciously sourced materials and personalised assembly.
Timeless design with nature at heart
Strong, durable and made to last. Every Type01 is brought to life through natural materials and planet-friendly production methods.
Easy assembly
Product details
Shelf parameters
244 cm
Compartment max load
113 cm
Shelf max load
40 cm
- Pre-mounted and click into place
- Weighted to self-close
- Feature extruded aluminum handles
- Easy to assemble - click into place
- Full extension runners for easy access
- Weighted to self-close
- Feature extruded aluminum handles
Shelf feet
- Adjustable to fit uneven floorboards
- Include felt pads to protect against scratching
What's included
- a personalised instruction manual
- a padded tool to disassemble your shelf
- wall fasteners and universal screws for safety purposes
- floor-protecting pads to protect floors
Click it together. Quick and easy.
Save valuable time and energy with our colour-coded, click-together assembly system. Just unpack the numbered boxes in order, follow the easy instructions and you'll have your shelf up in minutes.
If your shelf is higher than 60 cm or wider than 150 cm, we recommend fastening it to a wall. We also recommend fastening all shelves with a 24 cm depth. You'll find suitable hardware and wall plugs to do this inside your package. This step will make your shelf extra stable.
- Pre-mounted hinge hardware
- Click together to connect
- Pre-mounted slider hardware
- Parts easily click together
- Slide in the shelf to complete
- Max load bearing: 30 kg
Material and quality
Plywood is one of the strongest materials around. Our premium plywood is made of 13 layers of birch hardwood selected for its strength and beauty. We leave the edges exposed and hand-oiled to showcase the natural grain of the wood fiber.
13 layers of compressed birchwood
Hand-polished with toxin-free plant oil.
Safety practices and certificates
We pride ourselves on the Type01 being a safe, child-friendly shelf. Our wood is FSC- and PEFC-certified and comes only from ecological, sustainably managed forests. We use non-toxic glues and oils for added peace of mind.
Free returns
If for any reason you're not happy with your shelf, we'll pick it back up for free within 100 days for a full refund. No questions asked.
Free delivery
We professionally deliver your Tylko shelf for free – right to your doorstep. It will arrive in labelled flat pack boxes for quick, easy assembly.
We ship to the following countries: Austria, Belgium, Bulgaria, Croatia, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Ireland, Italy, Latvia, Lithuania, Luxembourg, The Netherlands, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, and the United Kingdom.
If for any reason you're not happy with your shelf, we'll pick it back up for free within 100 days and give you a full refund. No questions asked.
The Tylko guarantee
All of our furniture is made from high-quality materials that can withstand tough everyday use. We provide a two-year guarantee against failure and manufacturing flaws. For more details and conditions please read our Terms of Service.
We accept most major credit cards (Mastercard, Visa) and payments through PayPal.
Additional payment options differ depending on the country. All transactions are processed securely via our payment provider Adyen.
For more information regarding payment please read our FAQ.
Proud owners, perfect shelves
you comfortable
to fall in love
happy customers
personal service
Customer Reviews
Just what we needed, perfect!
After searching some time for a wall storage we liked, we found Tylko..... and it was exactly what we needed. They did what they promised, delivery, quality and everything fitted perfectly. See the picture, I mounted a Philips Hue light strip as well for the ultimate experience!
Wonderful !
It's always a bit tricky when ordering furniture online. You haven't actually seen the product and you have to base yourself on the projection. I have to say I have been absolutely stunned by the end result. I designed a custom bookshelf that would fit our wall on Tylko's webpage. It was super practical to tweak and play with. I placed the order and a month later : TA-DA ! A custom made bookshelf was delivered. Assembly was super easy and the quality is way up there. I'm super delighted and strongly recommend Tylko !
Absolutely delighted!
Excellent quality product and extremely straightforward to put together. Absolutely delighted with all aspects of our purchase and would definitely recommend to others.
Vinyl storage
Love my new unit for all my old vinyl. Ordering process was very easy and really liked the updates on how you were getting on. Construction was easy and finished product is great looking and super sturdy. Thanks!
Very happy
Great quality, straightforward assembly and the customer service was very helpful.
Great new shelves
Really happy with the shelves. The fit the space perfectly as designed and were really easy to assemble. The instructions and felted tool provided to protect shelves as you assemble were all really clear and well thought through. The labelling of boxes components and position of shelves made assembly really quick and easy, not your usual flatpack experience at all. Everything went together quickly and easily. The materials and finish are both excellent and really good value for money. Exactly what I wanted.
Great product and service
Love our new bookcase! Great quality, easy to build, clear instructions. Really happy with customer service, friendly helpful team! Delivery went smoothly, there were no delays and even the delivery driver was nice and helpful. Great after sales experience as well, 5 stars + customer care. Excellent overall service! Highly recommend and will definitely use again!
High quality product and great service - well done !
a very clever and well thought through innovation for bespoke shelving.. from time of order, to updates until delivery the whole process has been well thought through and professional. before finding Tylko we had requested several carpenters for quotations for installation of a fitted bookshelf but either we received no quote or it was for mdf without coating which we would have to arrange ourselves as well as provide a design template.. not what we wanted. Tylko not only offered us the chance for a bookshelf with exact measurements to our requirement but it also looks excellent and of high quality.. Tylko is not cheap but am pleased to say they have delivered in our opinion value for money and we will most certainly consider to order again or perhaps even invest in this exciting company should the opportunity come. Nb, it is worth noting we had installed our bookcase in an enclosed space which did not allow us to lie flat during build, we overcame this by building to a height (8th level) where we had floor space, then errecting and pushing into situ with final 2 levels completed on the vertical.. it proved not to be a big issue and we managed with a fit MM perfect.
Thats the best forniture of my life
Really high Quality and easy to assembly shelvs. 330cmx 245cm in 3 hours. We need another one!
Great design - to look at and to install
I was very curious to learn how easy it would be in reality to put the bookcases together. And I was more than happily surprised. In less than 1,5 hour I assembled 2 bookcases on my own, which would have taken me about double the time with an IKEA furniture. The manuals couldn't be more handy. The furniture doesn't only look great, it is also very solid and strong material. Moreover, the packaging has been designed in such a way that the cardboard boxes can be very easily folded, without any tapes to be cut or stitches to be removed. In short: this has been developed by great product designers who have looked much further than just making some nice furniture. By the way: I also loved the way you kept us posted on the delivery schedule.
Excellent quality and extremely well thought-through
My job is to design customer experiences and I'm usually extremely critical of the many sub-standard services out there, but my experience with Tylko was fantastic. From designing the shelf with the app to ordering it and then the updates of the status of my order, I felt I was in good hands. When the shelf arrived, the spediteur carried it all the way up to the fourth floor (I helped!). I was extremely impressed with how well packed everything was and the personalised instructions. Building the shelf was simple and it is really high quality. I'm absolutely a fan and hugely appreciate the effort that must have gone into getting everything right.
2nd unit ready to use in 1 hour!
This is the second Tylko product that I ordered. As for the first time, i like it very much and am very satisfied with the delivery including all the tracking messages. Very good packaging ensured that no damages occurred during shipping. Building the unit was easy after I understood how to position the parts supporting the drawers. It would have been easier to figure it out with more details on the instructions (not only labelling the pieces but also representing them on the instruction drawings). I highly recommend the Tylko units: fit in any interior, look stunning and very convenient! Thank you for the good work to the entire Tylko team!
Frequently asked questions
What's the difference between the Type01 and Type02 shelves?
The Type01 and Type02 both have the same modern aesthetic when it comes to pattern, grid, gradient and slant styles, as well as the same door and drawer design. The difference with the Type01 is that it's constructed from durable premium plywood with exposed edges showcasing the 13 layers of natural birchwood. The Type01 also features rounded aluminum handles that run along the edge of the door and drawers for an easy grab and safety.Check out our comparison page
How will I know if a shelf colour will suit my home?
We can send you a free colour sample pack in the mail. Let us know you want one by contacting [email protected] We can also provide the RAL/NCS codes for all of our colours.
Is a Tylko shelf safe to use in a child's room?
Absolutely. We only use sustainably sourced plywood and non-toxic glues and oils. The handles are also rounded to avoid any nasty bumps. The shelf itself is very sturdy and strong, so it won't sway, sag or topple over. But for added peace of mind, we provide wall mounts so you can attach it firmly to your wall.
How does the 100-day free return work?
Try your shelf for 100 days. If for any reason you're not happy with it, we'll pick it back up for free and give you a full refund. No questions asked.
Why do you offer only three row sizes?
Our three row heights are influenced by the common everyday objects of our customers – things like photo frames, vinyl records, oversized coffee table books and shoeboxes. The three heights (18 cm, 28 cm, 38 cm) have been carefully designed so that no matter how much you adjust or adapt them, they won't sway or sag under weight.
Find the one
Store Styslihly with Drawers
When you need a little (or a lot!) of extra storage space, look no further than Tylko's Chest of Drawers. For design-minded storage that fits any space, create your wooden chest of drawers in just a few clicks.
Designing a solid chest of drawers to suit the amount storage you need couldn't be easier. Whether a few drawers or more, the customizable sizing means your solid chest of drawers will fit, perfectly - tall or small.
How Does It Work?
Using Tylko's super simple design interface, you can easily create the perfect chest of drawers for any space. With a few taps and swipes, size, style, color and more can be decided. Need a few drawers? Done. Need a lot? Easy. Want it in black, white or grey? Done.
Our chest of drawers are a perfect option for bedroom furniture with convenient, concealed storage. And, thanks to Tylko's augmented reality, you can see your chest of drawers live in your space so you can decide before you buy.
What Can It Hold?
Your solid chest of drawers were designed with durability in mind. Made from beech for strength and stability, anything you want to store will be done so in style - while staying stable. You'll never see sag or sway, thanks to the rigidity of the strong wood. Tylko's steel snap-together connectors keep everything in place - and make it easy to assemble and break down your chest of drawers for years of use. No tools needed - simply snap together!
Whether you need a tall unit for a lot of storage or low one for just a little extra, you can be sure it will be solid - and stylish.
Will it hold large items?
It will! With drawer heights of 18, 28 and 38cm and variable widths that you can customize, you can be sure your woolly jumpers - or whatever you decide to keep in them - will fit in your drawers just as well as your smaller treasures. And, for optimal storage you can mix and match with open and drawered sections for a completely unique chest of drawers solution.
What About Scratches and Stains?
Not to worry. Your modern chest of drawers are finished with a high-quality laminate that means scratches and stains won't be an issue. High-traffic bedrooms or living rooms can mean bumps and brush-ups can happen, and with the finish quality of your chest of drawers you can be sure it will always look as good as the day you bought it.
Through years of use, your chest of drawers will retain their sheen while resisting scratches. Not only is each chest of drawers coated with laminate, the edges are hand-oiled to preserve the natural look of the beautiful wood - and add vital protection. With lesser-quality MDF and wood, corners can split or fray, or even swell when exposed to moisture. Tylko chest of drawers are designed to last for generations.
Where Can it Go?
Where can't it go? If you need a chest of drawers in your bedroom - or anywhere else- with Tylko you'll get something that fits perfectly every time. No shoving furniture around or measuring tight spaces - with the Tylko app you can point and decide!
By using augmented reality to see your chest of drawers live in your space, you can make sure it will fit - narrow, low, long or large. Most importantly though, it will look great, and fit perfectly. Just tap and swipe until you're ready to decide, then enjoy fast and free shipping on your new chest of drawers. And if for any reason you don't fall in love with them - just send them back, on us.
The data administrator is Custom Sp. z o.o.
The data administrator is Custom Sp. z o.o. with headquarters in Warsaw at ul. Mińska 25, building 63 (hereinafter also referred to as ""Administrator""). The administrator can be contacted via the [email protected] email address. You can contact the Data Administrator on all matters relating to the processing of personal data and the use of data processing rights.
Your data will be processed in order to inform about the Company's products.
Your personal data will be made available to entities that process personal data at the request of the administrator (including IT service providers, marketing agencies) - these entities process data on the basis of a contract with the administrator and only on the administrator's request.
Your personal data will be stored no longer than until you find out that you do not want to receive these materials.
You have the right to access your data and the right to demand their rectification, removal or restriction of their processing. At your request, the administrator will provide a copy of the personal data to be processed, but for any subsequent copies you request, the administrator may charge a reasonable amount resulting from administrative costs.
You have the right to withdraw your consent. Withdrawal of consent does not affect the lawfulness of the processing that was made on the basis of consent before its withdrawal.
To the extent that your data is processed in an automated manner in order to conclude and perform the contract or processed on the basis of consent - you also have the right to transfer personal data, i.e. to receive personal data from the controller, in a structured, commonly used machine-readable format. You may send this data to another data administrator.
You also have the right to lodge a complaint with the supervisory body dealing with the protection of personal data.
In order to exercise the above rights, please contact the data controller. Contact details are indicated above.
Providing personal data for the above purposes is voluntary.
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Want to reconsider? Your space deserves a great custom piece like this!
At-home assembly
Let us do all the heavy lifting for you.
- Professional assembly and set-up by our trusted team
- We'll take away all the leftover packaging
- Total price includes the items in your cart
This service is currently available for customers in Germany, Netherlands, Luxembourg, Austria and Belgium.
For more info, please read our FAQ
At-home assembly
Let us do all the heavy lifting for you.
- Professional assembly and set-up by our trusted team
- We'll take away all the leftover packaging
- Total price includes the items in your cart
This service is currently available for customers in Germany, Netherlands, Luxembourg, Austria and Belgium.
For more info, please read our FAQ
Shipping Time
We constantly update shipping time based on our factory production capacity and stock level.
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Invalid promocode. Please review and re-enter your code.


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James River Equipment Jobs
James River Equipment is your full-service equipment and tractor dealership dedicated to the needs of our agriculture, construction, forestry, commercial and residential customers in Virginia, North Carolina and South Carolina. Our services include equipment sales, parts, service and rentals. With 36 full service locations and over 100 field service vehicles, we stand ready for when you need us to keep you up and running!
Job Title & Tags
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subset: commoncrawl


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What are your thoughts on income inequality and its impact on people and countries?
Economic Inequality
Income Inequality in the United States
Do we really not have a topic on this one? I was able to come across some related topics but not the subject proper. I would estimate it is probably one of the most important issues in the United States right now, and in some other countries as well (I came across a few about Canada the other day).


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Congratulations to Steve. Thank you for participating in our Photo Contest to win one of our T-Shirts.
Fly-in Fishing in Canada Blog - Oak Lake Lodge
Steve, our latest Photo Contest Winner
Related Posts
"Big Fish" Guest Photos Congratulations for hauling out this massive Northern Pike
"Big Fish" Guest Photos 52 inch Muskie caught in June at Oak Lake Lodge
"Big Fish" Guest Photos Video taken by our guests.
"Big Fish" Guest Photos Bill catches a 31 inch walleye at Oak Lake Lodge
"Big Fish" Guest Photos Jim wins a t-shirt for his photo entry!
"Big Fish" Guest Photos Bob catches a 42 Inch Northern Pike at Oak Lake Lodge
- "Big Fish" Guest Photos (9)
- All Inclusive Fishing Trips (15)
- Archery Hunting, an option in September (3)
- Business Fishing Trips for Team Building (3)
- Catch and Release Fishing (3)
- Family Fishing Trips to Canada (10)
- Fishing in Ontario, Canada (10)
- Fishing Videos (3)
- Float Planes and Fly-in Fishing (2)
- Guided Fishing (6)
- Lodging and Meals (13)
- Majestic Nature Abounds (8)
- Muskie Fishing (1)
- Northern Pike Fishing (2)
- Smallmouth Bass Fishing (1)
- Trip Planning and Tips (15)
- Walleye Fishing (6)
- Walleye Shore Lunches (2)


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Welcome to the RU COOL satellite data image archive! On this web site you will find all of the available satellite data products we offer to research, industry, education and the general public. Satellite data are collected with SeaSpace L-Band and X-Band satellite dishes, both of which are on the top of the Institute of Marine and Coastal Sciences building on Cook Campus within the New Brunswick Campus of Rutgers, The State University of New Jersey.
Our L-Band dish (left) was installed in the Fall of 1992 and collects AVHRR data from three of NOAA's POES satellites and China's FY1-D satellite.
The X-Band dish (right) was installed in the Fall of 2003 and collects MODIS data from NASA's Aqua and Terra satellites, as well as ocean color data from India's Oceansat satellite.
Data are processed to into various products (i.e., sea surface temperature, ocean color, etc.) and then converted jpeg images are made for easy downloading and viewing. There are detailed product descriptions on each product page. Sea Surface Temperature is our largest image library, with imagery reaching back to 1993. Chlorophyll Concentration imagery goes back to 2001. Our other image archives begin in 2004.
On this main front page of our web site we will periodically post notes to our users regarding either the status of a particular product, or news regarding neat things going on with RUCOOL.
If you have questions or comments we would love to hear from you! Please contact us: Contact Form


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The Monmouth University Office of Purchasing, located on the lower level of Wilson Hall (W4), maintains control over purchases and assures compliance with University polices and procedures. It provides many additional services to the administration, staff, and faculty.
Primary functions include purchasing of equipment, materials, supplies and services as required, giving due consideration to price, quality, delivery, terms of payment, shipping services, and other factors that have an effect on end costs.
The Office of Purchasing also issues purchase orders, monitors receipts of goods, approves invoices for payment, and ensures that proper credit is received for returned goods. Purchasing handles all vendor contact with respect to returns, overcharges, damage claims, guarantees, and warranties. The staff assists departments and offices in developing multiple sources of supply for the purpose of competitive bidding and with technical and product information.
- Mark Miranda, Director of Purchasing, x3593
- Nan Merklinger, Purchasing Technician, x3476
- Susan O'Neill, Publications Production Coordinator, x3545
The Director of Purchasing is the authorized agent for the University in the procurement of goods and services.


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Wickham Flooring Click on the logo to the right for more info
Nothing comes close to our domestic floor collection for helping you design your dream decor. An unbeatable range of woods, colours and sizes. Choose from four different grades.
|3/4" (19mm)
|2-1/4" (57mm), 3-1/4" (83mm) or 4-1/4" (108mm) wide planks
|Nail down
|Select and Better, Canadian Plus, Natural and Rustic.
|17 colours in Maple, Walnut, Cherry, Birch, Ash or Red Oak.
|Satin and Semi-Gloss. UV-cured natural oil finish containing zero VOCs (volatile organic compounds).
|Finish - Additional
|Oxylust+ exclusive aluminum oxide finish for superior wear resistance
|Finish - Health Benefits
|Contains an antibacterial and anti-mould agent
|Residential Manufacturer's warranty
|35 Year
|FSC certified
|Specifications may change, please check with your local Windsor Plywood location to verify availability. Some products may be special order.
Submit the form below and we'll get back to you within 48 hours.
Availability of product varies by region.


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Rails of New England
by: Walter H Hunt & Gregory M Pozerski
Game Type(s):
Players: 2 - 5
Age: 12 +
Available Languages
Status: In-Print
Stock Number: 433
New England Rails is a business game set in 19th
century New England for two to five players. Each
player takes on the role of a would-be business tycoon
seeking to increase profits by expanding business holdings
and connecting them by railroad to major cities.
Players can make decisions to improve their own holdings,
and can directly affect each other by the use of
Action Cards. They may trade and buy business, as well
obtain Special Routes, Mail Contracts and State
Subsidies. The businessman who has the most money
by the end is the winner!


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Kid's A Hero
|Kid's A Hero
Mike at the first location
|GTA Advance
|Asuka Kasen
|Bedford Point, Staunton Island, Liberty City
|Ante Up
Kid's A Hero is a mission in Grand Theft Auto Advance, given to protagonist Mike by Yakuza co-leader Asuka Kasen from a building in Bedford Point, Staunton Island, Liberty City.
Asuka Kasen wants her niece, Yuka, returned from Colombian Cartel and, having heard of Mike's skills and unaware that Mike had kidnapped her, she employs him to return. Asuka is also having problems with other business ventures and asks Mike to first make a payment of $20,000 to a man in Belleville Park. In return, Mike asks for her help in discovering who murdered Vinnie. Mike drives to Belleville Park and hands over the money, before driving to Cisco's home in Aspatria, where Yuka is being held. He approaches Yuka's school bus and attempts to enter, however, the bus has been locked and some of Cartel guards have spotted him. Mike kills the bodyguards, collects the key and enters the school bus. Mike then drives back to Asuka's home in Bedford Point and returns Yuka to her aunt.
Asuka Kasen: My, my... Aren't you a handsome one? I wouldn't have expected it with the reputation you have of being a cold-blooded killer. I've heard a lot about you and if my sources are correct, you're the right man for the job I have. My niece has been kidnapped and her abductors have demanded an outrageous fee. I want you to track her down by any means necessary and rescue her.
Mike: Sounds like a job for a real professional. I hope you have more than stuffed animals and electronics to pay me with.
Asuka Kasen: If you do this for me, you will be rewarded beyond your darkest fantasies.
Mike: I'm not sure I like the sound of that but I'll do it under one condition. I'm tracking down those responsible for my partner Vinnie's death and I need any information you can get about the slime bags who specialize in making bombs.
Asuka Kasen: Mike-san, such tragedy makes me ache inside. I'll put my men on it right away. Now, back to the business at hand. There's a drop off spot for the initial sum of twenty thousand. Take this briefcase, make the delivery, and then see what you can find out about Yuka.
(Mike leaves the building)
Mike: This is my lucky day. I know where Yuka is being kept but what to do about this money?
(Mike drives to the initial location)
Thug: Don't make any sudden moves or you're a dead man! Put the briefcase down and then get the hell out of here. Tell Asuka she made the right move.
Mike: Easy on the trigger, man. You're not going to get away with this. You picked the wrong woman to cross.
Thug: Tell Asuka she's doing the right thing. Appeasement pays!
(Mike drives to Yuka's school bus in Aspatria and begins to approach)
Mike: Hmm, guards out front. Better try around back and rescue Yuka as soon as I can.
('Mike walks to the bus and attempts to enter)
Mike: Damn! One of the guards must have the key to the bus. Hmm... Looks like they spotted me.
(Mike kills the guards and collects the key to the bus)
Mike: Hmm... Looks like a key.
(Mike enters the bus)
Yuka: Ahh! Mike-sama, how are you? I knew you wouldn't leave me at the mercy of these mean men! You're my hero!
Mike: Hi Yuka. I hope they've been treating you okay. We're not in the clear yet. Hold on and I'll see you get back to your aunt's place safely.
Yuka: My aunt is going to be so happy to see me! She always has a present waiting for men who please her. I wonder why she places it on the bed and gets her whip out?
Asuka Kasen (pager): Kuso! Get back here as soon as you can. Asuka.
(Mike drives Yuka back to Asuka)
Asuka Kasen: Yokatta! You've returned my sweet, darling Yuka to me, Mike-san. You have my thanks... Perhaps I could express them more intimately inside my bedroom? Tell me, have you ever felt the crack of a whip across your back?
Mike: I can't say that I have, Asuka. I'm going to leave you to your toys and keep my skin intact. See you.
Post Mission Pager Script
Asuka Kasen: Be a darling and drop by my place, Mike-san. Asuka.
The reward for completing the mission is $10,000. The mission Ante Up is unlocked by completing the mission.
Video walkthrough
|GBA Version - GTASeriesVideos


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24 May 2013 at 5.25pm | 15 Comments
As part of our monthly series of recommended recordings from the Royal Opera House Shop, this month we take a look at releases from two great singers and a conductor with strong connections to the Royal Opera House.
Luciano Pavarotti - Pavarotti Sings Verdi (Decca)
With 2013 being Verdi's bicentenary, the recordings keep coming. None however, are going to touch Pavarotti sings Verdi for sheer class, élan and the sheer joy of singing. Decca had raided the vaults for this generous three–disc release, all taken from the great tenor's recordings made over the course of his career. Partnered in duet and ensemble by a host of the great names of 20th-century opera, Pavarotti is featured in extended extracts from 11 operas, and you can start at disc one or dip in and out as you prefer. We tend to forget the real Pavarotti that was there before – and after – the hype. Here he sings with unfailing beauty of tone, musical intelligence and – importantly – an emotional connect that is the preserve of the great singer. At the price of one disc, and with lavish packaging, you needn't think twice.
Sir Colin Davis and the London Symphony Orchestra - Der Freischütz (LSO Live)
Sir Colin Davis will be sorely missed, but we have a valedictory recording from him to cherish. Taken from concerts at the Barbican with his beloved LSO, his recording of Weber's Der Freischütz has just appeared. It's a very fine closing document to a great career – the LSO feels and plays this music as well as any orchestra, with a deeply committed and fully romantic approach that is truly lovely to hear. The cast are up to the task and with this great opera hopelessly under represented in the catalogue, this is well worth adding to your collection.
Ian Bostridge - Britten Songs (EMI)
As Verdi and Wagner are celebrated for their 200 years, so our own Benjamin Britten celebrates his 100. Ian Bostridge, surely the true heir to Peter Pears, takes on a large slice of Britten's song output in his new CD, Britten Songs. Accompanied for the most part by Antonio Pappano, he traverses among other songs the great Michelangelo Sonnets. The voice may have lost a little of its previous youthful ardour, but this is daring and exquisite artistry, with Bostridge and Pappano a meeting of equals searching for a musical truth. For the last six songs – Songs from the Chinese – Bostridge is hauntingly accompanied by the guitar of Xuefei Yang. This takes my vote as recording of the month – don't miss it.
What recently released recordings would you recommend?
Ian Bostridge sings during performances of Britten's Canticles in the Linbury Studio Theatre from 10 – 12 July 2013. All dates are sold out, though returns may become available.


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The combination of both internal supply and external relationships allows us to offer our clients a vast array of securities to trade:
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Our trading platforms have been designed with the professional trader in mind:
Our suite of Option Labs and advanced trading tools offer support to help you discover and implement optimal trading strategies. For example, our Probability Lab offers a practical way to think about options without the complicated mathematics, and the Option Strategy Lab lets you create simple and complex multi-leg option orders based on your own price and volatility forecast.
Trader Workstation (TWS) supports over 60 order types, from the most basic limit order to advanced trading to the most complex algorithmic trading, to help you execute a wide variety of trading strategies.
Our proprietary API and FIX CTCI solutions let institutions create their own automated rule-based trading system that takes advantage of our high-speed order routing and broad market depth.
Real-time market-risk management and real-time monitoring provide a comprehensive measure of risk exposure across multiple asset classes around the globe and real-time data that gives you the edge you need to react quickly to the markets.
IBKR's Order Management System (OMS) makes it easy to submit, stage, manage and track client orders whether they are received electronically via FIX, input directly by traders or placed over the phone.
Our User Access Rights system allows you to assign different account management functions to individual employees within your organization.
This one-stop solution is perfect for institutions looking to reduce costs and improve productivity with a fully integrated OMS/EMS setup. Order management, trading, research and risk management, operations, reporting, compliance tools, clearing and execution – all are available as part of our complete platform. The IBKR OMS offers a robust and customizable order management platform that you can use within your current multi-broker setup. Route orders to your existing execution providers, and allocate pre- or post-trade to multiple custodians using the IBKR OMS platform. With over 40 years of industry experience, we can easily configure our robust technology to fit the complex needs of your business.
Interactive Brokers Group (IBG LLC) equity capital exceeds $7.9 billion1. 18.5% of IBG LLC is owned by the publicly traded company, Interactive Brokers Group, Inc. and the remaining 81.5% is owned by our employees and affiliates.
Unlike other firms, where management owns a small share, we participate substantially in the downside just as much as in the upside which makes us run our business conservatively. We hold no material positions in OTC securities or derivatives. We hold no CDOs, MBS or CDS. The gross amount of our portfolio of debt securities, with the exception of US governments debt securities, is less than 10% of our equity capital.
PortfolioAnalyst, our online reporting and analysis interface, lets you analyze the performance of your account portfolio online by creating and saving customizable PDF reports based on a set of measurement criteria and optionally comparing the data to selected industry benchmarks.
Run and customize activity statements to view detailed information about your account activity, including positions, cash balances, transactions, and more.
Create statement templates called Flex Queries, which let you customize your activity data at the most granular level and download in XML or text format.
Save your statements in PDF or Excel format, or download them to a variety of third-party formats such as Quicken, Tradelog or CapTools.
Daily margin reports detail requirements by underlying.
TCA tracks the quality of your orders' transaction prices versus market conditions either at the time the orders were submitted or after the trade executes.
Tax Optimizer lets you change your tax lot-matching method to optimize your gains and losses.
We offer two types of Proprietary Trading Group account structures. Both account types provide institutions withthe flexibility of trade execution, clearing and prime broker services all within the same account.
Multiple users can view and modify other traders' activity.
Accounts are accepted from citizens or residents of all countries except citizens or residents of those countries or regions that are on the sanction list of the US Office of Foreign Asset Controls or similar lists, or other countries determined to be higher risk. Click here for a list of available countries.
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Please note that monthly activity and other minimum fees may apply.
Master, sub accounts and users are part of the same legal entity.
Funds are deposited in the master account and transferred between the master and sub accounts to control individual trader limits.
Initial margining is determined for each individual account, while maintenance margining is determined at a consolidated account level.
Master users can set trading limits on individual sub accounts based on order size and value.
Accounts are accepted from citizens or residents of all countries except citizens or residents of those countries or regions that are on the sanction list of the US Office of Foreign Asset Controls or similar lists, or other countries determined to be higher risk. Click here for a list of available countries.
Link to Administrators for the purpose of providing administrative services such as reporting to your sub accounts. Shop for registered Administrators at the Administrator Marketplace.
Please note that monthly activity and other minimum fees may apply.
For information on SIPC coverage on your account, visit www.sipc.org or call SIPC at 1 (202) 371-8300.


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Maximizing the Benefits of Zero-Energy Homes
A version of this article appears in the Solar & Efficiency Special issue of Home Energy Magazine.
March 19, 2007
There is an emerging trend in energy programs for new homes to shift from a focus on the use of individual high-performance components to a focus on overall home performance. Key examples of this trend include the MASCO Environments for Living program, the SMUD Zero-Energy Home program (see "Sacramento's Zero-Energy Community," Ready for Prime Time? Not all currently available technologies are suitable for use in residential programs that focus on realizing large incremental energy savings. Many successful products are energy neutral, provide modest energy benefits, or are targeted toward niche markets. There are four barriers to realizing large incremental energy savings in production homes:
To read complete online articles, you need to sign up for an Online Subscription.
Once an order has been placed there is an automatic $10 processing fee that will be deducted with any cancellation.
The Home Energy Online articles are for personal use only and may not be printed for distribution. For permission to reprint, please send an e-mail to firstname.lastname@example.com.


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Code: MDF
Strong and stylish, these frosted bags are perfect for trying something new!
All bags have the side and bottom gusset and with its choice of different colour handles its perfect for standing out from the crowd.
MDF1: 20.3 x 20.3 x 5 cm
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Shopping Bag, Gift Bag, Carrier Bag.
Foil printing available click here for prices. All of our printing is done in house. This allows us to personally ensure that your items are printed to the highest standard.
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Rather than contracting for trailer loads of bakery by-product and distillers grain, look first and intensely at feed efficiency. It's a much more effective way to improve income than adding lower-cost and lower nutrient quality ingredients to the diet.
Feeding more pounds of cheaper concentrates and losing pounds of milk, milk fat and milk protein won't improve your bottom line. You already knew that. So let's look a little closer at how to bring down costs.
For every 0.1% increase in feed efficiency, you can earn up to an extra 22 cents income per cow per day. That's especially true for Jerseys.
Simply put, feed efficiency is the milk produced per pound of dietary dry matter intake consumed. To make that calculation reflect the increased value and nutrient needs for milk fat and milk components, milk yield must be corrected for solids.
Solids-corrected milk accounts for differences in milk fat and milk protein, and allow producers with more than one breed to compare cows. The formula is: SCM = [(0.323 times pounds of milk) + 12.82 times pounds of fat) + (7.13 times pounds of true protein)].
So, for example, a group of Jersey cows producing 60 pounds of milk with 4.5% fat and 3.7% protein is equivalent to 69.2 pounds of 3.5% SCM milk.
To accurately calculate feed efficiency, refusals or leftovers also need to be accounted for. Most feeders know how much feed is delivered and how much is shoveled out of the feed bunks
Here's the formula for calculating feed dry matter intake: [(total feed delivered minus refusals) divided by number of cows per group] times the dry matter percent of the diet.
Okay, now you have SCM and dry matter intake. Feed efficiency is the pounds of SCM divided by dry matter intake.
Values should range from 1.0 to 2.0 with 1.5 to 1.7 as a target. Increasing feed efficiency just from 1.4 to 1.5 will increase income.
Other feed efficiency boosters
Many variables affect that value – milk output and milk component yields, days in milk, cow comfort, ventilation, grouping and forage quality. No surprises there, right? Anything that influences intake and production can influence feed efficiency.
* For instance, cows in early second lactation and mature cows are the most efficient. So, it may make sense to under-stock early lactation groups or give cows in early lactation the most comfortable stalls in the barn.
* Allocating higher digestibility forages to fresh and early lactation cows can also improve feed efficiency – and your bottom line.
* Getting cows bred back earlier also makes sense. That way, they spend more of their productive lives in the earliest parts of lactation.
* Improving forage quality by harvesting crops earlier and storing and sealing forages correctly can improve feed efficiency and reduce spoilage losses.
* Improving forage neutral detergent fiber digestibility just by 1% increases dry matter intake by 0.33 pounds per day and milk yield by 0.50 pounds. More milk per pound of intake boosts feed efficiency and your bottom line.
* Feeding higher NDF digestibility forages also improves rumen fermentation, resulting in more microbial protein and more essential amino acids for productive purposes. Microbial protein is the best and cheapest source of dietary amino acids.
* Boosting forage content with high NDF digestibility forages cuts feed costs without compromising milk and milk component yields – as long as physically effective NDF, starch and ruminally degradable protein needs of the rumen microbes are met.
* Feed additives that are cost effective include Rumensin, Fermenten and rumen-protected amino acids. Rumensin reduces synthesis of methane and energy waste in the rumen. Fermenten provides rumen-soluble nitrogen and carbohydrates for microbes and increases microbial yield.
Rumen-protected lysine and methionine reduce the need for ruminally undegraded protein such as bloodmeal and heat-treated soybean meal. And they do it without compromising milk protein synthesis. Reducing RUP in the diet makes more room for forage.
Feed sales people should easily be able to help you track feed efficiency and make simple, low cost suggestions to increase pounds of milk per pound of dry matter intake. That's what you're paying them for, right?
Carson and husband Steve partner in Harkdale Farms of Newbury, Vt. She's also a professor at Vermont Technical College.


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I had one of those epiphanies this week, where I thought to myself…Am I insane? Why have I never done this before? There must be some reason. Do I not bake with strawberries for a reason?… Honestly, the entire recipe I kept thinking, these aren't going to turn out because there must be some reason to not bake with strawberries. But nope, they turned out great and I'm still a bit confused why there don't seem to be more chocolate strawberry recipes. I always go for raspberries and chocolate! So anyway, these were on the somewhat not-too-guilty-pleasure side of baked goods. They were easy and decadent. My little people gave me dramatic ooos and ahhs and the house smelled too good for words!
Chocolate Covered Strawberry Scones
Makes 12
1 1/2 cups unbleached flour
1/4 cup raw sugar
1 1/2 tsp baking powder
6 tbs unsalted butter cut into small pieces
1/4 cup heavy cream
1 egg
1/2 cup strawberries, finely chopped
1/2 cup dark chocolate chips
Directions: Preheat oven to 375. Line 1 baking sheet with parchment paper. Place wax paper on a counter. In the bowl of an electric mixer, fitted with paddle attachment, place all ingredients, wet stuff on the bottom, dry stuff on top. Mix on low until dough JUST comes together. Do not overmix or scones will be tough(this is always a rule for scones or muffins). When dough is coming together, and mine felt quite wet, put onto wax paper and press into a 9 inch disk. Cut into 12 pieces and place on parchment lined baking sheet. (you can always flour a countertop and put the dough there, but I find it's easy to over flour and then you have scones that are a bit too dry). Bake for about 20-25 minutes until turning golden. Serve warm. These scones are definitely best the day they are made, but sufficed for breakfast the next morning too! Enjoy!


text: A STURDY TABLET CASE ESPECIALLY FOR CHILDREN 5 Excellent case for 7" tables, especially for children. Sturdy and zips up on both sides for easier closure and access. It is even possible to attach a lanyard to the zipper ends, and wear the case around the neck. LOVE IT!!! STURDY! Alameda, California 2015-09-18


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Empowering Educators. Inspiring Students.
Real science, real stories, and real data to engage students in exploring the living world.
Educator Voices
Hear how educators are using BioInteractive content in their teaching.
Using Gene Mapping to Introduce the Chi-square Test of Independence
Deepen your understanding of statistics with this article from Paul Strode about using our "Mapping Genes to Traits in Dogs Using SNPs" activity to teach the Chi square test of independence.
Data Points and Dead Zones in Coastal Ecosystems
Using the Identify and Interpret Strategy with Data Points
Want a simple but effective strategy for helping students interpret graphs? Robert Cooper unpacks how to teach students to interpret complex data figures, such as those used in BioInteractive's Data Points, in this article.
What a Wonderful World: Spiraling Statistics Throughout a High School Biology Course
Coherent lesson sequences driven by students asking questions about phenomena.
Resource Playlists
Ordered sequences of BioInteractive resources for teaching a course, unit, or lesson.
In-person professional learning workshops led by educators.
Science News
Articles that connect current events to BioInteractive resources.
Discover tools to help plan lessons and opportunities to support professional learning.
Keep up with the latest from BioInteractive!
New BioInteractive Ambassador Academy
A new professional development academy focused on growing the HHMI BioInteractive Ambassador community and building professional learning leadership capacity in science education.


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Human TNFRSF13B/TACI ELISA Kit PicoKine™
|96wells/kit, with removable strips.
|Sample Type
|cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
|Sample Volume
|100ul per well
|Assay Range
|Product Name
|Human TNFRSF13B/TACI ELISA Kit PicoKine™
|SKU/Catalog Number
|Storage & Handling
|Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|96wells/kit, with removable strips.
|Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TNFRSF13B/TACI. 96wells/kit, with removable strips.
|Cite This Product
|Human TNFRSF13B/TACI ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0986)
|Sample Type
|cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants that are not specified above or contact us to check for feasibility.
|Assay Range
|Expression system for standard: NSO; Immunogen sequence: S2-T166
|Cross Reactivity
|There is no detectable cross-reactivity with other relevant proteins.
|Antibody Clonalities
|Capture Antibody | Detection Antibody:
polyclonal antibody from goat|polyclonal antibody from goat
Assay Details
Kit Components
|Catalog number
|96-well plate precoated with anti-Human TNFRSF13B antibody
|lyophilized recombinant Human TNFRSF13B standard
|biotinylated anti-Human TNFRSF13B antibody
|Avidin-Biotin-Peroxidase Complex(ABC)
|sample diluent buffer
|antibody diluent buffer
|ABC diluent buffer
|TMB color developing agent
|TMB stop solution
|Adhesive cover
*Why there is no wash buffer? Our Avidin-Biotin-Peroxidase Diluent contains the detergent (TWEEN) normally present in other companies' ELISA kits. This saves you the step of having to wash with the special wash buffer and achieve similar or better signal to noise ratio. The wash can use regular wash buffers (PBS, TBS etc.) commonly found in labs.
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Human TNFRSF13B/TACI ELISA Kit PicoKine™
(TMB reaction incubate at 37°C for 15-25min)
Intra/Inter Assay Precision
|Intra-Assay Precision
|Inter-Assay Precision
|Standard deviation
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lot1 (pg/ml)
|Lot2 (pg/ml)
|Lot3 (pg/ml)
|Lot4 (pg/ml)
|Mean (pg/ml)
|Standard Deviation
|Sample 1
|Sample 2
|Sample 3
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Target Info
Protein Target Info (Source: Uniprot.org)
|Uniprot Id
|Gene Name
|Protein Name
|Tumor necrosis factor receptor superfamily member 13B
|Tissue Specificity
|Highly expressed in spleen, thymus, small intestine and peripheral blood leukocytes. Expressed in resting B- cells and activated T-cells, but not in resting T-cells.
|Alternative Names
|Tumor necrosis factor receptor superfamily member 13B;Transmembrane activator and CAML interactor;CD267;TNFRSF13B;TACI;
|Subcellular Localization
|Membrane; Single-pass type III membrane protein.
|Molecular Weight
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function
|Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T- cell function and the regulation of humoral immunity. .
|Research Areas
*You can search these to find other products in these research areas.
|Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor(TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand(CAML). TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.
Other Recommended Resources
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Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
Customer Q&As
Q: I found bubbles in the wells of my ELISA kit. Is this normal or will it have an impact on the performance of the kit?A: The bubbles in the wells is an uncommon issue, but it would not affect the results of your experiment.
We suggest to wash the plate to remove the bubbles as suggested in the protocol below prior to the assay:
- Wash the plate 3 times with the 1X wash buffer.
- Discard the liquid in the wells into an appropriate waste receptacle. Then, invert the plate on the benchtop onto a paper towel and tap the plate to gently blot any remaining liquid. It is recommended that the wells are not allowed to completely dry at any time.
- Add 300 µl of the 1x wash buffer to each assay well. (For cleaner background incubate for 60 seconds between each wash).
- Repeat steps a-b 2 additional times.
Q: There are precipitates in the sample diluent buffer. What is it?A: The precipitates are fatty substances contained in BSA. It would not affect the performance of the kit. If you want to confirm this, please contact Boster Support.
Q: I left this product out in ambient temperatures for around 5 days, will the kit be stable?A: Our kits should still be stable after being in ambient temperatures for around 5 days. It would not affect the performance of the kit. We suggest you to run the test and contact us if does not work as expected.
Q: What is the number of density of cells recommended for ELISA assay (for cell culture supernants and cell lysates)?A: The number of cells we recommened for cell culture supernants and cell lysates is 10^6 cells/mL.
Q: How many samples can I test on one ELISA plate?A: On each 96 well plate, we recommend running an 8-point standard curve with duplicate wells. With the remaining 80 wells, 40 samples can be tested in duplicate.
Q: The volumes of my samples are small, would it be possible to use 50µl/sample?A: There are two solutions for insufficient sample volume.
 1. Dilute the samples with the provided sample diluent buffer into 100ul.
 2. If 50ul of sample is added into a well, add 50ul of standard solution.
Q: Will this kit work on tissue samples?A: For tissue homogenates, endogenous biotin should be considered. Endogenous biotin in tissue homogenates might introduce false positive signal. Please run the test as described below to confirm if there is Endogenous biotin in the tissue lysate samples using the ELISA kit.
 • Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
 • If no signal is produced, then you can work on the tissue sample by using the kit.
Q: Is it possible to dilute 10X TBS stock that is 250mM Tris Base/Tris-Hcl, 27 mM KCl, and 1.37M NaCl to be used as the wash buffer?A: You can dilute the 10X TBS stock to 1XTBS and test the pH value of the 1xTBS.
 The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
Q: Would your kits work normally with samples from Wistar Rat?A: Our kits would work normally with samples from Wistar Rat.
Q: How can I store the dissolved wash buffer for a longer period of time?A: You can prepare a concentrated wash buffer (25X) which can be stored at 4°C for 1-3 months.
Q: Does the TMB color developing agent contain H2O2?A: Yes, the TMB color developing agent contain H2O2.
Q: Does this ELISA kit contain any product produced in humans or primates?
 Keywords: component, ingredientA: None of the components in our ELISA kits are produced in humans or primates.
Q: What is the volume of the recombinant protein control? What is its shelf life?
Keywords: expiration, storage, temperature, sizeA: The volume of the control is around 100pg. If unopened, the shelf life of the control is the same as the whole kit - "Store at 4˚C for 6 months, at -20˚C for 12 months." The reconstituted control can be stored at -20˚C for 2 days.
Q: Will it be okay to run the experiment if I accidentally used the wrong buffer (e.g. sample diluent buffer) for antibody dilution instead of the antibody diluent buffer? Keywords: dilution, replace, substitute, sample bufferA: Using the sample diluent buffer for antibody dilution instead of the antibody diluent buffer will negatively affect the test result to some extent. The values of both standard and sample might be lower than the normal values.
Q: The protocol says to use neutral PBS and provides a recipe. Could I use neutral PBS made with KCl or KH2PO4 (common constituents for most PBS recipes) instead? Keywords: protocol, alternative buffer, applications, reagent recipeA: Yes, it is ok to use common PBS recipes. We have tried many types of buffers with common constituents, and no significant difference was observed whatsoever.
Q: Why are my O.D. values different than your values on the datasheet? Keyword: troubleshooting, referenceA: We detected the kit in our lab and got our values on the datasheet before delivery, but protein activity will decrease as time goes on, so you may get lower O.D. values than ours. However, it should still be in a reasonable range and the standards can be used to calculate sample values. Good linearity of curve is more important than the actual numerical value.
Q: On the ELISA kit datasheet it says "HRP substrate TMB was used to visualize HRP enzymatic reaction." What is HRP and which part of the kit contains HRP? Keyword: kit components, reagents, protocolA: It means Avidin-Biotin-Peroxidase Complex(AR1103) and you could find it in Kit Components.
Q: Do you offer any ELISA kits that can work with whole blood samples instead of plasma or urine? Keyword: applicationsA: We test serum and plasma routinely, and there is very little difference between serum, plasma and whole blood. The whole blood also contains proteins we need to test. The kit can be used to test whole blood in theory.
Q: Does silicic acid (formula SiOH4) affect the results of the ELISA assay? Keyword: protocol, reagentsA: The acid may affect the binding of antigen to antibody, it is not recommended to use. That being said, it is unlikely to affect the reaction if the solution remains an overall neutral environment.
Q: Is there lot-to-lot variation of the ELISA kits? What are Boster's general services when I have questions about your kits? Keyword: technical support, help, customer serviceA: The NIBSC/WHO 1st International Standard is evaluated, we always test our kits before delivery, and customers can find the test result on the protocol. If the customer needs technical support from us to analyze their data, please contact us and we ask that you provide us the following information: the lot# and production date, and when did the customer detect the kit.
Q: The results of my standards do not look correct, what could be the problem? Keyword: troubleshoot, protocolA: Double check the protocol for plate washing. It should be examined on three aspects: 1) Did you make the washing buffer correctly? 2) How long did you let the buffer stay in wells? 3) What was the volume of the washing buffer added to each well? In addition, pay attention when adding sample to avoid contamination. And we suggest testing the standards again. Values of standards at low concentration are more affected than that at higher concentration, so customer can still get expected values at high concentration even if there is an error. If problems persist, please contact technical support with the catalog and lot number of your product.
Q: What is the well depth of the 96 well plates for the ELISA kits? Keyword: well capacity, product sizeA: The well depth is 300ul, and the max capacity is 350ul. The height of the well is 1.1 cm, and the internal size is 1 cm.
Q: The kit does not include wash solution, what should I do? Keyword: wash bufferA: Our Elisa kits do not come with wash solution, but we have included information about how to make washing buffer on the datasheet. Please refer to the "Material Required But Not Provided" section. We also offer washing buffer for sale separately (Phosphate Buffered Saline Powder SKU: AR0030).
Q: For your ELISA kits, are the capture and detection antibodies polyclonals or monoclonals? Keyword: antibody clonalityA: This information can be found for each kit under the "Properties" section, and you can find the immunogen sequence information in the "Overview" section.
Q: Do your ELISA kits come with sealants or plate covers? Keyword: storage, sequential useA: The kit can be used within a month sequentially if it's opened and stored at 4 degree. There is no need to use sealants, the plate can be packaged with aluminum foil bag, and for other reagents keep bottle tightly closed.
Q: What is the concentration of Sodium Azide in your Elisa kits? Keyword: preservativeA: Our Elisa kits contain 0.02% Sodium Azide. All of the components except TMB colour developing reagent and stop solution contains 0.02% Sodium azide as the preservative.
Q: Are there positive and negative controls available for my ELISA kit? Keyword: positive control, negative controlA: We can provide a recombinant protein as a control. It costs $50 per control and takes 2 weeks to manufacture. We cannot provide a positive or negative control in serum.
Q: Why do I get positive results for my knockout (KO) model when used as a control?A: The knockout (KO) model may contain truncated forms of the target protein which can be detected by the ELISA assay.
Q: How long do I soak my plate in the wash buffer?A: The plate should soak in 0.3mL PBS or TBS wash buffer for at least 1-2 minutes in an automated wash station.
Q: Can I use Tween in my wash buffer?A: While it is not recommended to use Tween in your wash buffer, small amounts (<0.1% concentration) may decrease background due to insufficient blocking.
Q: What plate type do I use to set up the microplate reader?A: Our plates are made with the Corning costar plates similar to the DNA-BIND 96 -well plates.
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the normal level of this protein in my sample of interest?A: We have reviewed literature and have gathered this information for most of our ELISA kits. You can find this information on the product page or contact us if you cannot find it. However this information is only suggestive and cannot replace pilot studies in determining the optimal sample dilution ratio.
Q: Is the plate separable? Can I use only part of the kit and save the rest for later? How many samples can I run with one kit?A: Yes the plate is separable if the kit says that there are removable strips. There are 12 strips of 8 wells each, all removable from the plate. The amount of samples you can run depend on a few factors. In the most common ELISA set up, you will use two strips for standards, and 10 strips for samples using duplicates, which let you run up to 40 samples per kit. Contact us if you have questions regarding other situations.
Q: Does the kit contain sample preparation reagents? How do I prepare my samples?A: Since different sample types require different reagents, we do not include them in the ELISA kit. However we do have each reagent mentioned in the file below available at very reasonable prices. Be sure to check them out. For sample preparation protocols please download the file below: https://www.bosterbio.com/media/pdf/Boster_Sample_Preparation_Protocols.pdf
Q: Can this ELISA kit work on brain tissue homogenate, cell culture supernatant, saliva, urine, serum, whole blood or any other sample type?A: In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. If you want dilution ratio suggestions on these sample types please contact our technical support. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.
Q: Can this ELISA kit react with the pro-form of the target protein? Can this ELISA kit react with an isoform of the protein?A: In general, unless otherwise specified, the ELISA kit is pan-specific, meaning that it will react with all different forms of the target protein if they share the majority of the target protein's sequence. The capture and detection antibodies are reactive to the entire sequence of the standard protein. You can find the sequence information of the standard protein in the "immunogen" section. For more information about the specificity of the kit for your particular experiment, please contact our technical support.
Q: Can this ELISA kit react with human, mouse, rat or other species?A: If the kit is reactive to another commonly used species (human, mouse, and/or rat), we would have listed it as a separate product. If you want to check cross-reactivity to a species that is not included in the 3 species listed above, please contact our technical support for more information. As a rule of thumb, if the sequences are 90%+ identical, there is a high chance of cross-reactivity for your species of interest.


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Happy weekend, the details of where they're from are listed below. Xx
And lastly I'd like to thank you all for your lovely recent comments - I appreciate them all <3. I've really been enjoying blogging lately and thank you for letting me know I'm not just talking to myself. Xx


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Liam Fox reported this story on Sunday, February 17, 2013 07:21:00ELIZABETH JACKSON: Papua New Guinea hardly ever features in the newspapers, broadcasts and websites of the world's big media organisations.
When it does it usually means that something terrible has happened, and that was the case earlier this month.
The story of a young woman who was burnt alive after being accused of being a witch sent shockwaves of horror and disgust around the globe.
In the past, Papua New Guineans have reacted angrily to such stories, saying they perpetuate the poor reputation of their country.
Here's our PNG correspondent Liam Fox.
LIAM FOX: It's pretty rare that different newspapers have exactly the same front-page headline.
But on the Thursday before last, both of PNG's daily papers screamed "Burned Alive".
The photographs accompanying the headlines were slightly different but showed the same scene. A crowed watched as tyres burn on a pile of rubbish beside the main street in Mt Hagen, the biggest town in PNG's rugged highlands region.
Burning rubbish is a common sight but what the photographs did not show was that underneath the tyres was the body of a 20-year-old woman. She'd been tied up, stripped naked, doused with petrol and set alight.
The local reports said the woman, identified as Kapari Leniata, was murdered after being accused of using sorcery, or black magic, to kill a young boy.
The boy died in hospital a few days earlier and, as is common in PNG when the cause of death is not easily apparent, his relatives suspected sorcery.
So they engaged a witch doctor, or glassman, as their called, to identify those responsible.
The witch doctor fingered Ms Leniata and two other elderly women, who were promptly rounded up and tortured with hot metal rods.
Locals told the newspapers the elderly women confessed that it was Ms Leniata who had taken the child's heart.
That was good enough for the boy's relatives who tied her up and burnt her alive.
Only two foreign media organisations have a presence in PNG, and once the ABC and the Australian Associated Press reported the incident, the story exploded. It went everywhere. Nearly every major news outlet on the planet ran it.
In the past, Papua New Guineans have reacted angrily to such stories. Every time a foreign journalist writes about sorcery killings or cannibals, people complain they're being stereotyped as savages living in a violent, lawless land.
But there was little complaining this time. Many locals were just as shocked, just as disgusted as people who were reading the story in Sydney or London.
On Papua New Guinea's biggest Facebook discussion group, Sharp Talk, one person wrote, "Oh my God. And we claim to be a Christian country. What happened to the Christian faith?" Another commented on the crowd that watched the woman burn: "I find this deeply troubling. How can people watch while this is going on? Where is the rule of law?"
In its editorial, the Post-Courier newspaper asked "whether we should be proud of ourselves as a nation".
This was a particularly disturbing example, but sorcery killings are common in PNG, especially in rural and remote areas.
The victims are mostly elderly women. They're easy targets for people looking to blame someone for a death or a misfortune they cannot easily explain.
Belief in sorcery, or sanguma as it's known, is pervasive. Even well-educated, self-declared Christians will tell you in hushed tones that black magic is real.
The brutal murder of the young woman has got the country thinking about the causes of sorcery killings and how to stop them.
Among the suggestions are economic development, legislative changes, and better-equipped police. All of which will take time.
And apart from recent messages of condemnation, it's still not clear that PNG's political leaders are prepared to meaningfully tackle the issue.
In the meantime, sorcery killings will continue and more women will die.


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uAppMatt necesitaba un nuevo diseño de logotipo y lanzó un concurso de diseño en 99designs.
Un ganador ha sido elegido entre 90 diseños de 34 diseñadores freelance.
uApp is a startup in the Mobile Application Generation market. There are many brands, businesses and identities that could benefit from having a custom mobile application. Unfortunately, most mobile application development is costly and takes several months. Updating applications follows suit. We have solved these problems by creating a web platform that allows anyone to easily create a personalized mobile application. Think of our platform as a production line for mobile apps. Because of our ability to create thousands of custom mobile applications in such a short amount of time, we do not need to charge tens-of-thousands of dollars to our clients.
On top of keeping our client's fresh content in their follower's hands, our applications also have the ability to generate money for our clients. Our mobile applications are of the highest quality so our clients know their content is being delivered to their followers in the best way possible.
The mobile application generation marketplace is very new. There are several groups that can benefit from having a mobile presence. Musicians, Bloggers, Restaurants, Artists, Celebrities, Writers...the list goes on. Anyone with online content to deliver to their following can benefit from a mobile application. This means our target audiences have a wide range style and personalities.
This is a somewhat challenging project because our target audience is so broad. Our logo needs to appeal to men and women, ages 18-48.
Our logo needs to compliment the high quality applications we produce. We have a web design firm that is working on our website. The attached file will show the design concept we are moving forward with. Ignore the content, our messages are being refined by our PR firm. The logo currently on the design concept image is merely a placeholder.
The logo should compliment the color scheme of design. We were thinking of using a light blue (sky blue) for the logo, but we are open to any concepts that work well with the website design. We would also like the logo to look good on a white background as well as a black (or dark grey) background, as there will be times when it will be presented separate from the website.
The spelling of uApp is crucial. We would like it to be spelled as you have seen in this brief: uApp. Within this concept, we feel that because the u and p's are rounded, a sharp pointed A does not look very good. Perhaps you could make it look good, but we were thinking that the A be somewhat rounded.
Our motto is "Your Content at the World's Fingertips".
We do not like overly shiny or glossy styles.
Descubre cómo funciona un concurso de diseño u obtén más información sobre nuestros servicios de diseño de logo.


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WISE-UP to Climate, a new project recently launched by the IUCN Global Water Programme, will demonstrate natural infrastructure as a 'nature-based solution' for climate change adaptation and sustainable development.
The project's name stands for 'Water Infrastructure Solutions from Ecosystem Services Underpinning Climate Resilient Policies and Programmes'.
WISE-UP will run over a four -year period and link ecosystem services more directly into water infrastructure development, starting with work in the Tana (Kenya) and Volta (Ghana-Burkina Faso) river basins. The project will be coordinated by a global partnership that brings together the International Water Management Institute (IWMI), the Overseas Development Institute (ODI), the Council for Scientific and Industrial Research in Ghana (CSIR), the University of Nairobi, the University of Manchester, the Basque Centre for Climate Change (BC3), and the International Union for Conservation of Nature (IUCN).
"The WISE-UP project will treat river basins as natural infrastructure, based on the ecosystem services they provide. In this way, river basin infrastructure planning and finance investments will include alternate portfolios of both built and natural infrastructure", said Mark Smith, Director of the IUCN Global Water Programme.
WISE-UP will develop the knowledge on built and natural infrastructure portfolios and demonstrate its optimized outcomes for poverty reduction, water-energy-food security, biodiversity conservation, and climate resilience.
For example, in the Tana river, Kenya's longest river, intensifying competition amongst water users is projected to intensify with the development of new hydropower plants, climate variability, as well as increasing urban, agricultural, and ecosystem demands. The Tana river is also the only river providing water to the lower delta inhabited by a poor and vulnerable population. River sediments and nutrients support the health of beaches and marine parks, critical contributors to the national economy.
Professor Eric Odada from the University of Nairobi and a Member of UNSGAB said that "in the Tana river basin, long-term climate resilient and sustainable solutions satisfying all water users and water developers will need to be implemented in order to avert conflict, livelihood losses, ecosystem degradation and economic hardship". A critical challenge is to adapt water management to climate change impacts while finding ways of equitably and efficiently balancing competing water demands.
An innovative element of the project is its foresight in linking water services from nature's infrastructure to investment, where investors and banking sectors can benefit from long-term sustainable returns from combined portfolios of built and natural infrastructure. WISE-UP will support a closer collaboration between experts working on infrastructure solutions and those active in development financing.
The implementing partnership consists of organisations with a wide variety of expertise. "This provides a really unique dimension to the project. We have natural resource scientists, engineers, computer modellers, economists, governance and political economists, water managers and climate change specialists all involved in the project. We hope to really challenge some of the misconceptions about ecosystems and their role in adaptation", said Matthew McCartney from IWMI. Linking and sharing analyses and tools from political, economic, social and technical research will better inform decision-making and financial outcomes for infrastructure development planning.
The project is funded by the International Climate Initiative (IKI) of the German Federal Ministry for the Environment, Nature Conservation and Nuclear Safety (BMU) and is projected to run for a 4-year period.
For more information, please contact email@example.com


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It is much easier to find 812-885-2705 info than you may have thought. Our free service lets you access data such as the mobile service provider, name, company name and other information of the caller.
Scammers can be stopped or at least blocked, but you need to be sure that it is a scammer. Check phone number 8128852705 and see if it is a normal company who called you by accident or a scammer. It may be that someone just mixed up phone numbers, and you receive calls from 812-8852705 by mistake. You do not need to pay a dime, our service is completely free. Access the largest phone numbers database in the US right now!
0 users reported this as unknown.
0 users reported this as scam.
0 users reported this as telemarket.
0 users reported this as harassment.
0 users reported this as debt collector.
1 users reported this as spam.
0 users reported this as survey.
0 users reported this as prank.
0 users reported this as political.
0 users reported this as positive.
Ellison , 2018-10-22 04:46:32
Phone number: 812-885-2705
Call type: Spam
Rating: -3
Mandy Harris, 2020-04-05 05:27:10
Number: (916) 398-1474
Call type: Scam
Rating: -5
Came as a text message pretending to be Netflix, threatening account cancellation in 24 hours.
Number: (614) 507-2111
Call type: Telemarket
Rating: -5
Car Warranty Annoyance!
Believe in science and facts, 2020-03-30 06:58:01
Number: (610) 600-1275
Call type: Political
Rating: -5
Scam Dotard Trump fake poll...probably Russian bot
Edwards, 2020-03-27 06:04:49
Number: (302) 202-7850
Call type: Spam
Rating: -5
unsolicited auto warranty services
Court Gentry, 2020-03-26 13:53:54
Number: (813) 308-4729
Call type: Scam
Rating: -4
Rental scam
Irene, 2020-03-25 06:42:55
Number: (503) 409-9587
Call type: Harassment
Rating: 5
Number: (567) 231-4085
Call type: Scam
Rating: -4
Keeps calling
Yes, 2020-03-21 21:02:59
Number: (877) 567-8692
Call type: Unknown
Rating: 0
I didn't answer it since I have no contact in US.
I suspect it was a scam call
Tina, 2020-03-18 06:53:34
Number: (814) 924-0102
Call type: Scam
Rating: -5
You have won a luxury prize !
calling so far 3 X today and five X on 3/17/2020 on home phone and once on husbands cell
Number: (833) 641-0316
Call type: Scam
Rating: -5
Robo Dialer - "this is about Social Insurance Number, please final notice..."


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Are you excited to deliver inspirational retail experiences to LEGO® fans of all ages?
Join the LEGO® Brand Retail team as a Sales Associate and provide inspirational guest experiences in an interactive and vibrant store environment. We are looking for energetic, enthusiastic individuals to join us.
Exceed our guests' expectations through fun and meaningful interactions
• Provide all customers with a warm welcome and exceed all expectations at every opportunity, interacting with the children
• Establishing needs, offering features and benefits and successfully closing the sale.
• Offer the Loyalty Card, Shop @ Home and any other services that are appropriate.
• Deal with customer complaints when they arise and escalate any difficult situations to your Line Manager.
• You will collaborate with other Sales Associates in order to provide the best possible service to all LEGO customers
• You will follow money handling procedures as per the guidelines and instructions provided by your line manager.
• You will comply with Health & Safety standards whilst on shift.
• When requested to do so by your line manager, you will contribute to the organization of the stockroom at all times and ensure that all available products are merchandised on the shop floor.
Do you have what it takes to inspire and develop the builders of tomorrow?
• Some experience in retail would be an advantage but is not necessary.
• Be an ambassador for the brand and the values it represents
• Qualified NVQ in Retail or equivalent is advantageous
• Excellent Customer Service Skills
• Good organizational, verbal and written communication skills are required.
• Computer literacy is an asset
• Experience of working with children is an asset
• Must be able to work flexible hours including evenings and weekends to support the store operations
Join the global family
If you are confident that you have what it takes to succeed in this part-time role, use the APPLY NOW button above or below.
Additional Information:
Candidates must be fully flexible (ie be able to work on weekends and evenings, Monday-Sunday)
Please can you include your availability in your application.
Bring it to life
I have never come across a company that cares so much about the personal and professional growth and development of its people in quite the same way as the LEGO® family do. When I joined the company 3 years ago, I soon discovered that imagination, creativity, fun, learning, caring and quality were alive and well in the workplace and not simply a set of values consigned to a poster on the canteen wall. No surprise then that I would encourage all of those talented individuals out there in the wider World to at least come see if this the company for them - Director of Store Operations Europe
Apply on company website


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Hockey East
The BU hockey task force: looking in all the wrong places
Over the past two days, I have had countless discussions regarding the task force report released by Boston University. I worked for over two years as the BU mens hockey beat writer for The Daily Free Press, and during that time, I was able to get to know the team a little better than your average fan, thus meaning people constantly ask me questions about the team.
However, after two players were arrested within 10 weeks of each other last winter, I was definitely just as troubled as the average BU student. Its hard to comprehend the types of crimes the two players were accused of, and it was hard to walk to class every day with news trucks lined up along campus.
I felt that BU had to do something about the arrests, but from the beginning, I did not believe the task force was the way to go. Here we had two arrests stemming from separate and completely different incidents of sexual assault, and rather than establishing an immediate plan for handling sexual assault on campus, the president decided to look into the culture of the hockey team. But to believe that sexual assault was an issue isolated to the hockey team was ridiculous, and the task force proved that to be true. It ultimately found nothing about BU hockey itself that caused the two incidents.
If you read the papers, youll see a different story. According to the media, the task force did find plenty wrong with the BU hockey culture. They have decided to run with the story that there is a culture of sexual entitlement among some BU hockey players and that the hockey players are held to a lower academic standard and isolated on campus from the rest of the BU students.
To a degree, those statements are true. When it comes to housing, the players are indeed somewhat isolated. They room with each other freshmen in Shelton Hall, sophomores on Bay State Road and juniors and seniors in Student Village I throughout their BU career. However, the hockey players share floors with other students, and the rooms of hockey players are scattered among different floors in each building.
And, just like every other competitive college athlete in this country, the BU hockey players do not all live up to the academic standards expected of your average BU student. Furthermore, like many college-aged students, the team tends to be sexually promiscuous.
I personally dont see any of those issues as a problem specific to the hockey team or the sexual assaults. The task force acknowledged as much. They said there were no NCAA violations, and the issues found with the hockey team at BU are commonplace at any college.
The root of the issue here has nothing to do with housing or academics or even hockey. The root of the issue is that college-aged men and women get drunk and do stupid things, and unfortunately, people end up being sexually assaulted. It happens nationwide, and it happened at Boston University. It wasnt just the hockey team. BU had multiple reports of rape and assault aside from just the two incidents related to the hockey team. Dont forget about the Peeping Tom in Warren Towers or the reported assault at Mugar library shortly after the second hockey players arrest.
BU had to respond last winter in some way in order to prove to people that they were doing something about the issue, but was forming the task force effective and was it targeting the right problem?
As an alumna of BU (I graduated in May), I can say from a students perspective, I do not believe BU targeted the sexual assault issue effectively. After much protest on campus last year including an online petition that over 1,000 students signed, the university finally created an on-campus center for sexual assault prevention and education. This happened 2 ½ months after the second arrest. The task force was created just days after the arrest. Since the on-campus center to combat sexual assault was created months before the results of the task force were released, I assume the sexual assault center was not the central focus of the task force.
While the task force investigated hockey culture, BU students continued to lack sexual assault protection on campus. After the second hockey player was arrested, one student attempted to use BUs only known system at the time for reporting some sort of assault: the Student Health Services crisis hotline. The student (who was testing the system and was never actually in danger) asked for resources available for victims of rape and sexual assault and was told by the person on the hotline that none were available through BU.
I personally noticed a lack of attention to the issue of sexual assault from BU. Last winter, a devastating fire in an off-campus apartment belonging to BU students ended up destroying all of the students belongings and seriously injuring at least one student. Those students called their parents before calling 911 about the fire. Less than a day after the fire, I received an email from BU briefing me on fire safety.
Why did that never happen with the sexual assault issue? Never once did I get an email educating me on what to do in case of sexual assault on campus. Who should I call? I still have no idea. What is the best way to avoid situations in which I may be sexually assaulted? It seems like common sense to me on how to avoid situations in which I may be at risk of sexual assault, but it also seems like common sense to me to call 911 in case of a fire.
And so while its all well and good that the task force has come up with all kinds of suggestions as to how to better academically integrate the hockey team with the rest of the university, it still seems to me that the task force missed the point. It appears as if BU conducted this whole task force in order to say theyd done something but swept the issue of sexual assault on-campus to the side.


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This is an amazing look at a period of our history that wasn't that long ago involving the challenges loggers faced every day to earn their wages. Remember this the next time you think your job is demanding.
Before chainsaws were invented, the logging industry in the United States & Canada was a seriously challenging occupation and we are only talking about 125 years ago. In the Pacific Northwest there were forests full of monster trees and cutting them down was done by hand.
Look at the length of the two-man hand saw and heavy duty axes they used to drop these tremendous trees. It is almost inconceivable to think of cutting a tree this size with a hand saw.
The work required very strong men (and horses) working long days for minimal pay. Could you imagine doing this to earn a living?
After a tree was finally felled it took a week or more to cut it up into sections that could be managed (somehow) and transported by train to a lumber yard.
Maneuvering the logs down the mountain to the train was a complex job. Many men likely lost their lives doing this dangerous work. One slip and a hunk of wood as big as a hotel is rolling your way! The other question that begs an answer is how did they get those logs onto the flatbeds of that train?
Hollowed out logs became the company's mobile office. Can you imagine stacking such logs to build a log home? Two courses would produce a 30′ ceiling. Maybe that's why it was easier to hollow out a tree.
A long time before anyone ever thought of a "mobile home or RV" hollowed out logs were also used to house and feed the logging crews.
We are accustomed to our modern conveniences like electricity and gasoline powered chainsaws, and it is always such a mind-boggling experience to see how such monumental tasks were performed before these conveniences appeared on the scene.This article first appeared at the Log Home Directory in August, 2012.


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Miss Buncle's Book
Gleebooks is Sydney's leading independent bookseller. We have been in business for over 40 years, and online since 1996. Branches in Glebe, Dulwich Hill, Blackheath; foyer stall at Roslyn Packer Theatre, Walsh Bay,
I've enough books by the bedside table at the moment to dispense with the rickety little bi..
Of course this would have been your regular end- of- summer 'how good was that' , Scomo ..
Well, as our cover shows September is our annual chance to highlight the Indigenous Literacy Found..
Subscribe to the gleemail, our weekly events and book news bulletin.
© 2006-2013 gleebooks.com.au All Rights Reserved Privacy | Copyright


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Diplomatic Measures
Mo'mor the Breaker at Telaar in Nagrand has asked that you find and speak with Lantresor of the Blade in the Burning Blade Ruins.
- Hear the Tale of the Blademaster
The huntress's agents send news of the Boulderfist leader: He is an orc! An ancient orc that passed through the Dark Portal in the time of our fathers and has since returned to his homeland. I wish for you to track down this orc and try to convince him to pull back the Boulderfist ogres. He resides on an upper platform in the Burning Blade Ruins to the east. Good luck, <name>!
Be warned, he is a blademaster...
You will also receive:
- 6 (if completed at level 120)
- 150 reputation with Kurenai


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I am new to this game and was unable to find a previous post or guide on google that addresses this specific issue.
I tried to use an Alfa AWUS036NH on my Mac laptop that has Mac OS 10.13 High Sierra. I tried downloading drivers from the Alfa website for 10.13 and 10.12 and one driver for BearExtender but that was unsuccessful.
The LED Indicator is blue. The system information utility on the Mac does not display any devices connected to the USB. The Alfa utility icon in the upper right corner does not display any networks and when I click Show Wireless Utility.... nothing happens.
Any feedback or help is greatly appreciated!


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Belgian Waffle
MAX VG Cloud Chaser Vape Juice Sub OHM E-LIQUID
Shake And Vape:
There is no nicotine content in our juices, just add the nicotine shots provided and mix to your desired strength. If you purchase with 0 nicotine shots, you will receive 120ml of our liquid in a 120ml dropper top bottle. If you purchase with 1 nicotine shot this will make a 1.5mg liquid and you will receive 110ml of our liquid in a 120ml bottle, and 1 x 10ml nicotine shot. If purchased with 2 nicotine shots this will make 3mg, you will receive 100ml in a 120ml bottle + 2 x 10ml nicotine shots.If purchased with 4 nicotine shots this will make 6mg, you will receive 80ml in a 120ml bottle + 4 x 10ml nicotine shots.
Most of our juices are best steeped for 1-3 weeks dependent on flavour to bring out the best taste, however some juices are instantly full of flavour ready to just shake and vape.
- 80/20 Cloud Chaser Juice
- 80% Vegetable Glycerine
- 20% Propylene Glycol
I have an effective age verification process that satisfies due diligence as described in ""The Business Companion Guide"" for online age verification checks and i will NOT SELL to persons that are under 18 years old.
|Rockstar Vape


text:
Doe Run Resources, the nation's largest lead producer, agrees to pay a $7 million fine and spend around $65 million to resolve violations of federal clean air standards. The St. Louis-based company will also close its Herculaneum, Mo. lead smelter, which emits 30 tons of lead annually. [Greenwire]
A pod of dolphins is captured and slaughtered by residents of the Japanese village made notorious by the documentary film "The Cove," environmental groups say. Breaking from their practice in past years, they set some of the juvenile dolphins free. [Associated Press]
Roma communities in France, subject to a controversial crackdown, are increasingly turning to scavenging metals from discarded e-waste and potentially exposing themselves and their children to toxic compounds, an investigation finds. [The Ecologist]
Over protests, a wild horse roundup begins in Colorado. [Associated Press]
Hurricane Paula forms in the Atlantic and heads for Mexico. [Weather Underground]


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Terry Denery, MathWorks
Sam Mirsky, MathWorks
In this presentation, you'll learn how to use MATLAB and Simulink to develop an embedded control system including implementation and testing on hardware. The demonstration emphasizes how to design, simulate, and test a complex system that incorporates multiple domains—such as mechanical, electrical, and hydraulic—that are typically isolated across different software platforms and not simulated in a common framework. Starting from underlying physical principles, we build models that can expand and accelerate the development process through simulation. You see how to simulate the controller and the plant together, optimize the control system, and generate code for HIL testing—all before building a prototype. We demonstrate the integration of simulated and real components to enable hardware-in-the-loop (HIL) testing in support of early verification and validation (V&V) against top-level requirements. You will learn how to:
Recorded: 20 Mar 2013


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John Sylvester is an internationally published travel, nature and landscape photographer based in Prince Edward Island, Canada. He specializes in photographing the people and places of Canada, with an emphasis on the Atlantic region.
John's Editorial clients include: Coastal Living, Yankee, Country Gardens, Ranger Rick, Canadian Gardening, Canadian Living, Canadian Geographic, Outdoor Photography Canada, Readers Digest, UP!, Explore, Saltscapes, Imperial Oil Review, Country, Our Canada, Harrowsmith Country Life, National, TIME, Backbone and National Post Business Magazine.