text:
PU1 - Power Supply Unit, 110 VDC, 75 W
The 3U 12HP power supply unit PU1 is a plug-in device for 19" systems (e.g. VMEbus, CompactPCI) and is especially designed for railway computer systems, being fully compliant with EN 50155.
Main Features
Technical Data
- Standard Euro rack size 19"
- Integrated heat sink for extremely low thermal stress to temperature sensitive components
- Glued parts for better vibration resistance
- Overtemperature and overvoltage shutdown
- Short circuit protection
- Remote on/off
- LEDs: green LED indicates correct input voltage, yellow LED indicates operating mode
- Fixed switching frequency 200kHz
- Reverse polarity protection by internal protector
- Thermal Warning (TW)
- Operating criteria: Overtemperature shutdown -5K (Risk of overtemperature exists.)
- Output TW: Output with NPN transistor, emitter connected with V out-; max. current 30mA
- Hold-up Time
- According to EN 50155 Class S2 for P out 75W
- Supply Fail (FAL)
- Output FAL: Isolated optocoupler output with NPN transistor
- Current: 5mA
- Voltage max.: 5V
- Pins: Open collector FAL, emitter FAL connected to V out-
- Isolation: FAL-V in: 1.500 Vrms
- Sense (S)
- Provides compensation of voltage drops (max. 0.2V each line) between power supply and load
- Pins S+ and S- of converter must be connected to load
- Shutdown interrupt (SD-INT)
- SD-INT in: 24V
- SD-INT out current: 5mA
- SD-INT out voltage: 5V
- SD-INT out pins: Collector pull-up resistor 10K to V out+
- SD-INT out isolation: SD-INT in to SD-INT out: 1.500 Vrms
Input Characteristics
- Nominal voltage input: 110V DC
- Wide input range: 0.7 x nominal voltage < nominal voltage < 1.25 x nominal voltage (according to EN 50155)
- Max. input voltage range: 0.6 x nominal voltage < nominal voltage < 1.4 x nominal voltage with full functionality for 0.1 s, no damage for 1 s (according to EN 50155)
- Voltage on threshold: 1.1V
- No load input current: 15mA
Output Characteristics
- Output: 5V/75W
- Accuracy: ±0.5%
- Voltage deviation: 12%
- Rise time: 80ms
- Overvoltage protection: 1.2V
- Overcurrent protection: 1.08A min., 1.15A max.
- Overtemperature shutdown: 101°C min., 105°C typ., 107°C max.
Input and output via DIN 41612 plug connector, type H15
Electrical Specifications
- Isolation
- Input/output: 1500V eff
- Input/case: 1500V eff
- Output/case: 500V eff
- MTBF: tbd. @ 50°C (derived from MIL-HDBK-217F)
Mechanical Specifications
- Dimensions: 3U, 12HP, 128.4mm height
- Weight: ca. 1395g
Environmental Specifications
- Temperature range (operation): -40..+85°C
- Temperature range (storage): -40..+105°C
- Shock: according to IEC 68-2-27
- Continuous shock: according to IEC 68-2-29
- Vibration: according to IEC 68-2-6
- Protection
- Class of protection: Class I, EN 60950
- Degree of protection: IP20 (inserted in rack), DIN 40050 (IEC 529)
- Pollution degree: 2 (EN 60950)
Data Sheets


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Several applications require the maintenance of human embryonic stem cells (hESCs) in the absence of a murine embryonic fibroblast (MEF) feeder layer. This protocol describes the culture of hESCs on matrix-coated plates in the presence of MEF-conditioned media (MEF-CM).
In this system, hESCs are maintained at high density. At confluence, the cells reach 300,000–500,000 cells/cm2.
- Dulbecco's Modified Eagle Medium (D-MEM) (Cat. no. 10569-010)
- Fetal Bovine Serum (FBS), ESC-Qualified (Cat. no. 10439-024)
- D-MEM/F-12 (1X) with GlutaMAX™-I (Cat. no. 10565-018)
- KnockOut™ Serum Replacement (KSR) (Cat. no. 10828-028)
- MEM Non-Essential Amino Acids Solution, 10 mM (Cat. no. 11140-050)
- β-mercaptoethanol, 1000X (Cat. no. 21985-023)
- Gibco® Mouse Embryonic Fibroblasts (Irradiated) (Cat. no. S1520-100)
- Basic Fibroblast Growth Factor (bFGF) (Cat. no. PHG0264)
- GlutaMAX™-I (100X) (Cat. no. 35050-079)
- Collagenase Type IV (Cat. no. 17104-019) for enzymatic passaging or StemPro® EZPassage™ Tool (Cat. no. 23181-010) for mechanical passaging
- Cell Scraper (Falcon, Cat. no. 353085)
- Dulbecco's PBS (DPBS) with Calcium and Magnesium (Cat. no. 14040-133)
- Dulbecco's PBS (DPBS) without Calcium and Magnesium (Cat. no. 14190-144)
- Geltrex® LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix (Cat. no.
A1413301 or A1413302) or CELLstart™ CTS™ substrate (Cat. no. A1014201)
- Attachment Factor (Cat. no. S006100)
- Stem Cell Tested 0.22 μm pore size sterile filters (Nalgene, Cat. no. 564-0020 or 156-4020)
- BD Falcon T175 TC-Flasks (Falcon, Cat. no. 353112)
- 37°C water bath
- Appropriate tissue culture plates and supplies
Note: As an alternative to D-MEM/F-12 (1X) with GlutaMAX™-I, KSR, and bFGF, you can use the KnockOut™
ESC/iPSC Media Kit (Cat. no. A1412901).
10 μg/mL bFGF Solution (1000 μL)
- To prepare 1 mL of 10 μg/mL bFGF solution, aseptically mix the following components:
|10 μg
|DPBS without Calcium and Magnesium
|980 μL
|10 μL
2. Aliquot and store at –20°C for up to 6 months.
10 mg/mL Collagenase Type IV Solution
- Add D-MEM/F-12 to Collagenase Type IV to make a 10 mg/mL solution. Gently vortex to suspend and
filter sterilize the solution. This solution can be aliquoted and frozen at –20°C until use.
- The 10 mg/mL working solution can be used for 2 weeks if properly stored at 2–8°C (store in aliquots to avoid repeated warming).
MEF Medium (for 100 mL of complete medium)
- To prepare 100 mL of complete MEF medium, aseptically mix the following components:
|89 mL
|FBS, ESC-Qualified
|10 mL
|MEM Non-Essential Amino Acids Solution, 10 mM
|1 mL
|β-mercaptoethanol, 1000X
|100 μL
2. Complete MEF medium can be stored at 2–8°C for up to 1 week.
Pluripotent Stem Cell (PSC) Culture Medium (for 100 mL complete medium)
- To prepare 100 mL of complete PSC Culture Medium, aseptically mix the following components:
|79 mL
|20 mL
|MEM Non-Essential Amino Acids Solution, 10 mM
|1 mL
|bFGF (10 μg/mL)*
|40 μL
|β-mercaptoethanol, 1000X
|100 μL
2. Complete MEF medium can be stored at 2–8°C for up to 4 weeks.
Preparing MEF-Conditioned Medium (MEF-CM)
- Cover the whole surface of each new culture vessel with Attachment Factor (AF) solution and incubate the vessels for 30 minutes at 37°C or for 1 hour at room temperature. For MEF-CM generation, a T-175 flask is recommended.
- Using sterile technique in a laminar flow culture hood, completely remove the AF solution from the culture vessel by aspiration just prior to use. Coated vessels may be used immediately or stored at room temperature for up to 24 hours.
Note: It is not necessary to wash the culture surface before adding cells or medium.
- Plate 9.4 × 106 Mitomycin C-treated or irradiated MEFs in a T-175 flask coated with AF and containing
30 mL of MEF medium.
- The following day, replace the MEF medium with 90 mL of PSC Culture Medium.
- Collect the PSC Culture Medium, now considered MEF-CM, from the flasks after 24 hours of conditioning for up to seven days in a row.
- Each day, filter sterilize the collected MEF-CM with a 0.22 μM filter. Filtered MEF-CM can be stored at
–20°C until use.
- At the time of use, thaw the MEF-CM in a 37°C waterbath, and freshly supplement it with additional bFGF (20 ng/mL).
Coating Culture Vessels with Geltrex® LDEV-Free, hESC-Qualified Basement Membrane Matrix
- Thaw a 5-mL bottle of Geltrex® LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix at 2–8°C overnight.
- Dilute the thawed Geltrex® solution 1:1 with cold sterile D-MEM/F-12 to prepare 1-mL aliquots in tubes
chilled on ice. These aliquots can be frozen at –20°C or used immediately.
Note: Aliquot volumes of 1:1 diluted Geltrex® solution may be adjusted according to your needs.
- To create working stocks, dilute a Geltrex® aliquot 1:50 with cold D-MEM on ice, for a total dilution of 1:100.
Note: An optimal dilution of the Geltrex® solution may need to be determined for each cell line. Try various dilutions from 1:30 to 1:100.
- Quickly cover the whole surface of each culture dish with the Geltrex® solution (refer to Table 1).
- Incubate the dishes in a 37°C, 5% CO2 incubator for 1 hour.
Note: Dishes can now be used or stored at 2–8°C for up to a week. Do not allow dishes to dry.
- Aspirate the diluted Geltrex® solution from the culture dish and discard. You do not need to rinse off the
Geltrex® solution from the culture dish after removal. Cells can now be passaged directly into MEF-CM onto the Geltrex® matrix-coated culture dish.
Note: CELLstart™ CTS™ substrate may be substituted for Geltrex® hESC-Qualified Matrix (see the Appendix).
Table 1. Volume of Geltrex® hESC-Qualified Matrix Required
|Culture Vessel
|Surface Area (cm2)
|Volume of Diluted Substrate (mL)
|6-well plate
|10 cm2/well
|1.5 mL per well
12-well plate
|4 cm2/well
|750 μL per well
|24-well plate
|2 cm2/well
|350 μL per well
|35-mm dish
|10 cm2
|1.5 mL
|60-mm dish
|20 cm2
|3.0 mL
|100-mm dish
|60 cm2
|6.0 mL
- Label the Geltrex® matrix-coated dish with the passage number from the vial, the date, and user initials.
- Remove the vial of hESCs from liquid nitrogen storage using metal forceps.
Note: If the vial is exposed to ambient temperatures for more than 15 seconds between removal and thawing, transfer the vial into a container containing a small amount of liquid nitrogen.
- Roll the vial between your gloved hands until the outside is free of frost. This should take ~10–15 seconds.
- Immerse the vial in a 37°C water bath without submerging the cap. Swirl the vial gently.
- When only an ice crystal remains, remove the vial from the water bath, spray the outside of the vial with
70% ethanol to sterilize, and place it in hood.
- Pipet the thawed cells gently into a sterile 50-mL conical tube using a 5-mL sterile pipette.
- Slowly add 10 mL of MEF-CM drop-wise to cells in the 50-mL conical tube. While adding the medium,
gently move the tube back and forth to mix the hESCs. This reduces osmotic shock to the cells.
- Rinse the vial with 1 mL of MEF-CM and add to the 50-mL conical tube with cells.
- Transfer cell suspension into a 15-mL centrifuge tube. Centrifuge the cells at 200 × g for 5 minutes.
- Aspirate and discard the supernatant.
- Resuspend the cell pellet in sufficient volume of MEF-CM according to Table 2 by gently pipetting the cells up and down in the tube a few times.
- Aspirate the excess Geltrex® solution from the prepared dish and slowly add the thawed colonies onto the dish. Move the dish in several quick, short, back-and-forth and side-to-side motions to disperse cells across the surface the dish.
- Place dish gently into the 37°C, 5% CO2 incubator and incubate the cells overnight.
- The next day, remove the spent medium with debris using a sterile serological pipet and transfer it into a prepared Geltrex® matrix-coated dish. You can use this dish as a backup in case there is a problem with the main dish.
- Add fresh MEF-CM to each dish according to the volumes in Table 2. Place both plates gently into a 37°C, 5% CO2 incubator overnight.
- Examine cells under the microscope and replace spent medium daily from both plates. If feeding more than one plate, use a different pipette for each well to reduce the risk of contamination. Colonies may not be visible for up to a week.
Table 2. Volume of MEF-CM Required
|Culture Vessel
|Surface Area (cm2)
|Volume (mL)
|6-well plate
|10 cm2/well
|2.0 mL per well
|12-well plate
|4 cm2/well
|1.0 mL per well
|24-well plate
|2 cm2/well
|0.5 mL per well
|35-mm dish
|10 cm2
|2.0 mL
|60-mm dish
|20 cm2
|4.0 mL
|100-mm dish
|60 cm2
|10.0 mL
When to Split Cells
In general, split cells when one of the following occurs:
- hESC colonies are becoming too dense or too large.
- Increased differentiation occurs.
Split Ratio
- The split ratio can vary, though it is generally between 1:2 and 1:4. Occasionally, cells will grow at a different rate and the split ratio will need to be adjusted. A general rule is to observe the last split ratio and adjust the ratio according to the appearance of the hESC colonies.
- If the cells look healthy and colonies have enough space, split using the same ratio. If they are overly dense and crowding, increase the ratio. If the cells are sparse, decrease the ratio. Cells will need to be split every 4–10 days based upon appearance.
Enzymatic Passaging Using Collagenase
You may passage cells via the enzymatic method as described below, or mechanically as described in the
following section.
- Label a new Geltrex® matrix-coated dish with the cell line name, the new passage number, the date, the split ratio, and user initials. Return the dish to the incubator.
- Under a dissecting microscope, remove differentiated colonies from the dish to be passaged.
- Aspirate the spent medium from the dish with a Pasteur pipette, and rinse the dish once with Dulbecco's PBS (DPBS) without Calcium and Magnesium.
- Add Collagenase Type IV (10 mg/mL) solution to the dish containing hESCs. Adjust the volume of
Collagenase Type IV for various dish sizes (e.g., 35-mm dishes require 1 mL of Collagenase IV).
- Incubate the dish(es) for 5–7 minutes in a 37°C, 5% CO2 incubator. Note that the incubation times may vary among different batches of collagenase; therefore, examination of the colonies is needed to determine the appropriate incubation time.
Note: As an alternative to Collagenase Type IV, you may use Dispase at a concentration of 2 mg/mL and incubate the dish(es) for 2–3 minutes in a 37° C, 5% CO2 incubator.
- Stop the incubation when the edges of the colonies are starting to pull away from the plate (see Figure 3).
- Aspirate the Collagenase Type IV Solution with a Pasteur pipette. Remove the collagenase carefully without disturbing the attached cell layer.
- Add MEF-CM to each dish. Use a 5-mL pipette to gently blow the cells off the surface of the dish while
pipetting up and down. Make sure to pipet gently to minimize the formation of bubbles.
- After the hESCs have been removed from the surface of the well, pool the contents of the wells into a 15-mL conical tube.
- Using a 5-mL pipette, add MEF-CM to the dish to wash and collect any residual cells. Pipet up the medium and cells, and then add the collected cells to the 15-mL tube.
- Pipet cells up and down gently a few times in the 15-mL tube to further break up cell colonies. Pipet
carefully to reduce foaming.
Note: Avoid making a single cell suspension.
- Centrifuge at 200 × g for 5 minutes, and then aspirate the supernatant from the hESC pellet.
- Resuspend the pellet with an appropriate amount of MEF-CM (refer to Table 2). This is dependent on the split ratio and the number of dishes used.
- Mix the cell suspension well with a 10-mL pipette. Be careful not to break up the colonies too much or cause bubbles in the media.
- Add appropriate volume of cell suspension to each dish. Return the dish to the incubator.
- Move the dish(es) in several quick, short, back-and-forth and side-to-side motions to disperse cells across the surface of the dishes.
- Incubate cells overnight to allow colonies to attach. Replace spent medium daily.
Note: While cells are attaching, be careful when opening and closing the incubator doors to avoid disturbing the even distribution of cells on the surface of the wells.
Mechanical Passaging Using the StemPro® EZPassage™ Disposable Cell Passaging Tool
- Replace the medium in the dish containing the cells with fresh MEF-CM.
- Under a laminar flow hood, open the package containing the EZPassage™ tool and remove the tool.
- Hold the culture vessel in one hand and pull (roll) the EZPassage™ tool across the entire dish in one direction. Apply gentle but firm pressure so that the entire roller blade touches the dish and maintains uniform pressure during the rolling action.
- Keep rolling the EZPassage™ tool parallel to the first pass until the entire dish has been covered.
- Rotate the culture dish 90°, and then repeat rolling the cell layer as described above.
- When you are finished, discard the EZPassage™ tool and do not reuse. Use a cell scraper to lift cell clusters off the plate, if necessary.
- Using a serological pipette, rinse the dish with medium so that the cut colonies are suspended in the medium.
- Transfer the medium containing the colonies to a 15-mL sterile tube.
- Seed the cell colonies on dishes plated with mitotically inactivated MEFs at an appropriate density.
- Place the plates into a 37°C, 5% CO2 incubator. Shake the plates gently to evenly spread out cells.
- In the PSC Culture Medium, DMEM/F12 containing GlutaMAX™-I (Cat. no 10565-018) can be substituted with the following alternatives:
KnockOut™ DMEM/F-12 (Cat. no. 12660-012)To prepare 100 mL of complete PSC Culture Medium using KnockOut™ DMEM/F-12, aseptically combine the components listed in the table below
Component Stock
Volume KnockOut™ DMEM/F-12 (Cat. no. 12660-012)
1X 78 mL KnockOut™ SR (Cat. no. 10828-028)
20% 20 mL GlutaMAX™-I (Cat. no. 35050-061) 200 mM 2 mM 1 mL MEM Non-Essential Amino Acids Solution
(Cat. no. 11140-050)
10 mM 0.1 mM 1 mL bFGF (Cat. no. PHG0024) 10 μg/mL 4 ng/mL 40 μL β-mercaptoethanol (Cat. no. 21985-023) 1000X 1X 100 μL
KnockOut™ DMEM (Cat. no. 10829-018)To prepare 100 mL of complete PSC Culture Medium using KnockOut™ DMEM, aseptically combine the components listed in the table below.
Component Stock
Volume KnockOut™ DMEM (Cat. no. 10829-018)
1X 78 mL KnockOut™ SR (Cat. no. 10828-028)
20% 20 mL GlutaMAX™-I (Cat. no. 35050-061) 200 mM 2 mM 1 mL MEM Non-Essential Amino Acids Solution
(Cat. no. 11140-050)
10 mM 0.1 mM 1 mL bFGF (Cat. no. PHG0024) 10 μg/mL 4 ng/mL 40 μL β-mercaptoethanol (Cat. no. 21985-023) 1000X 1X 100 μL
Alternative bFGF pack sizes
|Product Name
|Cat. no.
|Product Size
|FGF-basic (AA 1-155) Recombinant Human
|10 μg
|FGF-basic (AA 1-155) Recombinant Human
|25 μg
|FGF-basic (AA 1-155) Recombinant Human
|100 μg
|FGF-basic (AA 1-155) Recombinant Human
|1 mg
Dissociation Enzymes/Tools for Harvesting iPSC
|Dissociation Enzyme /Tools
|Suggested concentration
|StemPro® EZPassage™ tool
(Cat. no. 23181-010)
|Manual passaging
|Sterile, disposable tool
|StemPro® Accutase®
(Cat. no. A11105-01)
|Monolayer of cells post passage,
Dissociation into single cells
|1X ready to use (1–2 minutes
incubation at 37°C)
|Dispase (Cat. no. 17105-041)
|Colony-like morphology post passage
|2 mg/mL (2–3 minutes
incubation at 37°C)
|TrypLE™ Express
(Cat. no. 12604-021)
|Dissociation to single cells
|1X ready to use
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
MAN0006682 05-Mar-2012

subset: commoncrawl


text:
I've been learning java for around a month now, I've used mybringback tutorials and beginner newboston tutorials on youtube alongside many online websites and some books. I understand many of the concepts though I'm getting to a point where I feel I need to start working on some project of my own to get a firm hold of the basics so I can move on, most tutorials on the net at this stage teach some game programming or simple maths problems. Do the users have any other ideas of projects I could have a go at. I don't really have much of an interest in game programming or particular math problems, security is something I find interesting though this requires advance knowledge of languages.
Thank you for taking the time to read my post.
If you feel I should stick it out with some game programming please say.


text:
Cindi Fleischer necesitaba un nuevo diseño de logotipo y lanzó un concurso de diseño en 99designs.
Un ganador ha sido elegido entre 70 diseños de 12 diseñadores freelance.
AntiCupcake Company
I grew sick of cupcake companies springing up everywhere with beautiful but tasteless treats. My mission is to provide consumers with delicious, mind-blowing flavors of cheesecakes (anti-cupcakes) in smaller portions that aren't overpoweringly sweet like many traditional cheesecakes. I like to think out of the box to develop flavors like Gorgonzola Fig, Maple Pecan, Avocado, and Fireplace S'mores cheesecakes. The logo should try to reflect my breaking-the-mold mentality. The whole story is here: http://blog.anticupcakeco.com/about/
Young, sophisticated, foodies with an open mind for unusual cheesecake flavors. Target age group is 20s-30s.
This logo will be used for the website, business cards, and on the bakery boxes themselves.
-It should be recognizable and preferably coincide with the color pallet of the current website-in-progress: http://www.anticupcakeco.com.
-Should attempt a connection with cheesecakes and if the design include a graphic of a cheesecake, it should be recognizable to a reasonable person as a cheesecake.
-No cupcake bashings. I realize the name is anti-cupcake, but I would like the logo to focus on cheesecakes. :)
-Preferably also include "Cheesecake and Bake Shop" as a subtitle (but not required if the logo itself somehow conveys this)
-Stay simple with warm clean colors:pastels, creams, blues, etc. No neons or cold colors please.
-Preferably can be seen and recognized from a distance- this means the logo will have to stand out in some way with an outline or visual cue.
I am open minded to all designs that portray the required emotions. Please be creative!
Descubre cómo funciona un concurso de diseño u obtén más información sobre nuestros servicios de diseño de logo.


text:
SouthHadley, Massachusetts
|This article is a stub. Help us to expand it by contributing your knowledge. For county and town page guidelines, visit U.S. County Page Content Suggestions.
South Hadley is a town in Massachusetts. It was formed in 1753 and was previously called Hadley. It can be found in Hampshire county and is a part of the Northampton registry of deed district. For more information, contact the county at 116 Main St., South Hadley 01075. On the attached map, South Hadley is shown as 17 in Hampshire county.
For information about the state of Massachusetts see Massachusetts Family History Research.


text:
> Our smart city car
> Big on quality and value for money
> A generous, flexible interior space, available with 3 or 5 doors
> The next step in ŠKODA's design language, available in Hatchback or Estate
> Outstanding interior space
> Best small car - 2017 WhatCar? Car of the Year awards
> All-round practicality and safety for families
> Hatchback body style with fantastic interior space
> Up to 1490 litres of boot space
> Dynamic and fresh design
> Spacious passenger leg room and boot
> Up to 1,380 litres of boot space
Find an Approved Used RAPID SPACEBACK
> Our All-New family hatchback
> Sporty, generously spacious and versatile
> Compact family car of the year - 2019 AutoExpress New Car Awards
> Its spacious, premium interior makes it perfect for families
> Available in Hatchback, Estate, Scout or vRS.
> Estate Car of the Year - 2018 Auto Express New Car Awards
> Style and space redefined
> Our flagship family executive, available in Hatchback or Estate
> Auto Express 'Best Family Car' 2017
> Sturdy and versatile
> Rugged design
> Family orientated practicality
> Our all-new compact SUV
> Spacious, stylish & comfortable
> 5* European New Car Assessment Programme (Euro NCAP) Safety rating, plus full rating for cyclist protection.
> Our stylish mid-sized SUV with a big sense of adventure
> Self-assured and spacious design
> Mid-size SUV of the year - Auto Express New Car Awards 2018
> Our first car to offer five or seven seats
> Design, space, functionality and connectivity combined
> Best Large SUV - Auto Express New Car Awards 2018
You can find your nearest Approved Used ŠKODA retailers in just a few taps or clicks. Just enter your postcode, town name or, if you know it, the retailer's name, and we'll show you your five nearest in a few seconds.


text:
The Ovi brand of mobile services will be one of the first casualties of Nokia's deal with Microsoft. As Nokia plans to bring Windows Phones to market exclusively, the Ovi brand name is going away. The services will stick around under Nokia's name. Even that, however, is likely a measure designed to get customers to stop thinking about Nokia's mobile products as something separate from the Nokia brand.
It's important to note that none of the services are going away: Even though the name "Ovi" will go away, all of the services will stay up and active. Everything "Ovi" will be renamed to "Nokia" by the end of 2012. For example, the Ovi Store will become the Nokia Store, and Ovi Maps will become Nokia Maps. All of these, however, will likely either go away entirely or be minimized when Nokia starts loading Windows Phone 7 on its devices and users will have access to Bing Maps and the Zune Marketplace.
Ovi started in 2007, and was supposed to be a Symbian response to Apple's iTunes App Store. The service has – much like Symbian – floundered regardless of the platform's popularity outside of the United States. According to PC World, some of the Ovi services were so unpopular that Nokia stopped offering them, like Ovi Music Unlimited, which launched in 33 countries and eventually only wound up in six. Still, Symbian remains remarkably popular outside the United States, although that popularity is eroding quickly due to the rise of Android devices and the Apple iPhone.
At the same time, Nokia hasn't announced when its first Windows Phone will hit the market, and when those newly re-branded Nokia services will be replaced by Microsoft's own services bundled with Windows Phone 7, if ever. Nokia hasn't wasted any time dropping Symbian though, cutting 4,000 jobs altogether and then transferring 3,000 more to Accenture – along with the rights to Symbian.
Read more at Nokia Conversations via PC World


text:
Taf Toys North Pole Activity Book
- Soft baby book, with two sides for two developmental stages, with crinkle filling for ultimate sensation
- To use in cot, pram and for tummy-time play
- For Easier Development and Easier Parenting!
Development Values
- Side 1: High contrast colors, baby safe mirror and face drawings develop baby's senses and emotional intelligence side 2: Rich colors,textures,3D activities including a teether and a play pocket, to develop baby's fine motor skills
- The book stands up alone to help encourage tummy-time play
- The book is fully crinkled, helps to teach baby the idea of cause & effect
- 2 crinkling sides for 2 development stages includes: baby-safe mirror, crinkling shapes, 3D activities and textured fabrics. with a star teether toy and moon pouch
- Stands up alone for tummy-time play
- Size: 14 x 85cm/5.5" x 33.5"
Order Acceptance
When you place your order with us online, you will receive an automated e-mail notification verifying the products you have ordered and shipping methods. Please allow 1 - 2 working days for the processing of your order.
Method Of Payment
We currently accept payments via major credit card providers (VISA/ MASTERCARD), Apple Pay, and Google Pay, using our secured server to ensure your privacy and security.
Discount Codes
Discount codes (if applicable) can be keyed in the checkout payment page.
Delivery Methods
Courier Delivery
We offerr free local courier delivery for orders with minimum spend of $50, unless otherwise indicated. For orders below $50, a delivery charge of $3.99 will be applicable.
Delivery duration is approximately 2-3 working days. Delivery service is trackable. Tracking info is available in the order shipment email. Delivery service by the logistics partner is from Monday to Saturday, 9AM - 10PM.
We aim to deliver to as many places as we possibly can. However, due to certain restrictions, we are unable to deliver to the following places:
Tuas Link, ST Kinetics, Changi Terminal 1-3 and all Boulevards, Resorts World Sentosa, Army Camps, Seletar Airport, Shipyards, Pulau Ubin, Country Clubs, Changi Cargo Complex, SATS Inflight Catering Centre 1, ST Kinetics, Turf Club Avenue, Jurong Island, and any other offshore islands.
Baby Cot Delivery and Assembly
Baby cots purchased will be entitled to free delivery and assembly. Customer will be contacted for arrangement of the delivery and assembly. If customer fails to fulfill the arranged delivery date without informing us prior to the delivery date, a redelivery and reassembly fee of $80 will be charged.
Self Collection
You can collect your order from our office after you receive an email from us stating that it is ready for collection. Please allow 1 - 2 working days for the processing of your order.
Collection Location:
Infantino Enterprise Pte Ltd
6 Yishun Industrial Street 1 #02-16/17
Northview Bizhub Singapore 768090
Collection Hours: 9am - 6pm (please avoid 12.30 - 1.30pm).
Return Policy
All purchases are non-refundable. Exchangeable merchandises must be returned within 7 days of receipt of goods for exchange. Requests for exchanges are allowed only if you have received an incorrect or defective item.
Merchandise must be in its original condition (never been used) and in its original packaging with all the packing materials, manuals and registration cards intact. We will not accept dirty, washed or used products. Change of mind is not a valid return/exchange reason. A refund will only be processed if the item(s) have run out of stock for exchanges.
We apologize if you received a defective/faulty item. Please inform the Customer Service Team by taking a snapshot of the defective area and sending it to us at email@example.com
We apologize if we have sent you an incorrect item. Please inform the Customer Service Team at firstname.lastname@example.com with the order number and affected item name.
Please email us at firstname.lastname@example.com to request for the return before sending/bringing the item to us. Enclose your order/invoice number and/or a copy of your return request approval e-mail in the return parcel.
All furniture and items that have been assembled will incur a 30% restocking fee. Some products are considered special order items and, unfortunately, are not returnable. If this is the case it will be clearly noted on its product page.
Please note that we cannot accept returns for the following classes of products: feeding accessories, breast pumps, bathing accessories, lotions and powders, diapering accessories, diaper pails, potty seats and wipes, video/audio tapes, and undergarments.
All returns & exchanges are subjected to final approval.
All baby gear (i.e. strollers, car seats, booster seats, baby cots, playpens or high chairs) by Bonbijou are covered under a one (1) year limited warranty period. Your product is warranted against manufacturing defects under normal usage. Any negligence or mishandling while using the product will void the warranty.
In addition, the following are not applicable during the one (1) year limited warranty period:
- Seat cushions (applies to all strollers, car seats and high chairs)
- Canopies, storage baskets, handle grips and wheel surfaces
- All wear and tear due to usage
- Flight mishandling
- Baby cot legs
By registering, the customer expresses consent that the data provided during the warranty registration may be collected, stored and used by Infantino Enterprise Pte Ltd for the purpose of fulfilling the warranty, marketing and other purposes.
Please note that warranty can only be registered online here.


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donatemycard necesitaba un nuevo diseño de logotipo y lanzó un concurso de diseño en 99designs.
Un ganador ha sido elegido entre 222 diseños de 80 diseñadores freelance.
Donate My Card
We are launching a new technology that will take the over $8 billion in unused gift card value sitting in drawers and wallets in the US and donating them to charities. Both retail gift cards and prepaid VISA cards can be donated. What major charities? Red Cross, March of Dimes, Salvation Army, United Way, etc. What Major retailer cards? .Best Buy, Home Depot, Gap, VISA prepaid debit, and more.
Our system is totally digital...the cardholder goes online (or their 3G Smartphone) and once registered, can simply put the card number into our secure database, accept the terms and designate the charity they want the funds to be donated to.
We will have a very high profile Advisory Board, and a rock solid management team. We intend to quickly be the largest faciliator of donations to charities in the country.
Initially our logo is designed for our corporate/charity/celebrity audience initially, but ultimately this is a consumer logo as we roll out donatemycard.com.
Our brand needs to stand for integrity, compassion yet modern. We use technology to build the bridge between unused value and charities that need the money. As a consumer brand, it has to speak to both the givers of these gift cards and the recipients...who are usually young and brand -conscious.
****UPDATE 4/29/10 3:30pm PST**** Please do not submit any logo with the letters "DMC" in it. Too close to RunDMC and we don't like acronyms. Thanks!
Descubre cómo funciona un concurso de diseño u obtén más información sobre nuestros servicios de diseño de logo.


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Login Required
You need to have a Pokémon Trainer Club account to save your Favorite Pokémon!Log In Sign Up No Thanks
Cinccino's body is coated in a special oil that helps it deflect attacks, such as punches.
Their white fur feels amazing to touch. Their fur repels dust and prevents static electricity from building up.
Contact with the Pokémon may cause infatuation.
Powers up the Pokémon's weaker moves.
The Pokémon Company International is not responsible for the content of any linked website that is not operated by The Pokémon Company International. Please note that these websites' privacy policies and security practices may differ from The Pokémon Company International's standards.
Pokemon.com administrators have been notified and will review the screen name for compliance with the Terms of Use.
Your request could not be completed. Please try again. If the problem persists, please contact Customer Support.


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The fact that I can plant a seed and it becomes a flower, share a bit of knowledge and it becomes another's, smile at someone and receive a smile in return, are to me continual spiritual exercises. Leo Buscaglia
So what do you think of the video blog? I'm actually enjoying doing it because I feel closer to you. It's also easier for me to express myself if I just speak what's on my mind rather than typing it out. I feel self-conscious, like a bug under a microscope, but it is a lot easier to express myself that way. I just kind of start the webcam and speak whatever is on my mind. I don't really plan out what I am going to talk about. I am not going to video blog everyday because it takes more time to create a video blog than just sitting down and typing my blog out. Anyway, I hope you are enjoying it. You probably think I am crazy, who knows, maybe I am? LOL.
With that I leave you with Madonna…
Related articles
- Why to Video Blog? A Beginner's Guide to Video Blogging (mokshis.com)
- Video Blogging (joejeeber.com)


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The IHM Chronicles Series
I had hardly been there for a few minutes or so in my first college, the Institute of Hotel Management, Kovalam, and life was suddenly beginning to look like a beach holiday. More
On My Nightstand
Everything Beautiful Began After by Simon Van Booy
Sea of Poppies by Amitav Ghosh
Room by Emma Donoghue
Essential Rumi | Coleman Barks
History of Art by H. W. Janson
Tweets and Chirps !
Follow @sonisomarajan on Twitter


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Carmen Miranda Costume
Rate this costume:
21 votes
|1 comment · add a comment
Carmen Miranda lives!
|Costume type:
|Costumes for Women
This homemade costume for women entered our 2011 Halloween Costume Contest.
A word from Lauren H., the 'Carmen Miranda' costume creator:
As soon as I found an old photo of Carmen Miranda, I knew her colorful style and punchy attitude would make a perfect Halloween costume project. I had the photo up in my bedroom for months and built the costume piece by piece for just as long. My favorite little DIY secret with this one is the black "sequins" on the top. For the life of me, I couldn't find any sequins for sale that were big enough or shiny enough to match the photo. To substitute, I got plain black photos printed up for under a buck and just cut them into sequins! The makeup and the 'tude were just the sexy icing on the sassiest cake my Halloween party had ever seen.


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The first cover for "The Best Gifts," a lovely picture book by Marsha Forchuk Skrypuch, featured a close-up of an infant nursing at a breast. Predictably, it caused a backlash among the sort of people who have trouble seeing breasts as attractive and sexy but functional.
A new edition of "The Best Gifts" features artwork by Elly MacKay. Her illustration shows a dark-hued woman with a baby nursing discreetly, which is the way, let's face it, that most breastfeeding moms arrange themselves and their infants.
And after all that, are you expecting "The Best Gifts" to be a militant pro-nursing book?
Well, it's not.
It certainly condones breastfeeding and mother's milk, but it's really about the intangibles behind the things that loved ones give to one another. A scrapbook covered with a piece from a beloved baby blanket, a bedtime story book with a well-loved patina, a bit of something (like sandalwood) that evokes the scent of home — what makes them valuable is the association more than the things themselves.
Hopping back on the soapbox for a moment, let me note that breastfeeding is one of the few gifts that combines the tangible and intangible. It's not always easy, but the benefits to a baby (and mother) are far-reaching.
While "The Best Gifts" won't win a Newbery or Caldecott award, it is a lovely little book, perfectly suitable for a family with a newborn.
"The Best Gifts" by Marsha Skrypuch, illustrated by Elly MacKay (Fitzhenry & Whiteside, $18.95). Ages 4 and up.


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Home Front Posters Of The Second World War
From 'Coughs and Sneezes Spread Diseases' to 'Keep Calm and Carry On', the Home Front posters of the Second World War have become part of British culture. Some were striking design classics and others more prosaic, but all give a fascinating insight into the trials and tribulations of life in Britain during the Second World War. This was the first 'total war', with civilians as essential to the war effort as soldiers, so posters instructed people on everything from cooking and salvage to how to speak, and even think, about the war. This book tells the story behind these posters: how they were designed and produced, what it was hoped they would achieve, and what the British public really thought of them, as well as the role of 'Britain's secret weapon' humour. Looking at famous images and forgotten gems, it is essential reading for anyone interested both in life on the Home Front and the design of the times.
Freedom is in Peril
The Ministry Says
All in this Together
Big Brother is Watching You
Now that the War is Over
Places to Visit
Further Reading