text:
- Mini-Review
- Open Access
Genome features of Pseudomonas putida LS46, a novel polyhydroxyalkanoate producer and its comparison with other P. putida strains
© Sharma et al.; licensee Springer 2014
- Received: 14 March 2014
- Accepted: 16 March 2014
- Published: 22 May 2014
A novel strain of Pseudomonas putida LS46 was isolated from wastewater on the basis of its ability to synthesize medium chain-length polyhydroxyalkanoates (mcl-PHAs). P.putida LS46 was differentiated from other P.putida strains on the basis of cpn60 (UT). The complete genome of P.putida LS46 was sequenced and annotated. Its chromosome is 5,86,2556 bp in size with GC ratio of 61.69. It is encoding 5316 genes, including 7 rRNA genes and 76 tRNA genes. Nucleotide sequence data of the complete P. putida LS46 genome was compared with nine other P. putida strains (KT2440, F1, BIRD-1, S16, ND6, DOT-T1E, UW4, W619 and GB-1) identified either as biocontrol agents or as bioremediation agents and isolated from different geographical region and different environment. BLASTn analysis of whole genome sequences of the ten P. putida strains revealed nucleotide sequence identities of 86.54 to 97.52%. P.putida genome arrangement was LS46 highly similar to P.putida BIRD1 and P.putida ND6 but was markedly different than P.putida DOT-T1E, P.putida UW4 and P.putida W619. Fatty acid biosynthesis (fab), fatty acid degradation (fad) and PHA synthesis genes were highly conserved among biocontrol and bioremediation P.putida strains. Six genes in pha operon of P. putida LS46 showed >98% homology at gene and proteins level. It appears that polyhydroxyalkanoate (PHA) synthesis is an intrinsic property of P. putida and was not affected by its geographic origin. However, all strains, including P. putida LS46, were different from one another on the basis of house keeping genes, and presence of plasmid, prophages, insertion sequence elements and genomic islands. While P. putida LS46 was not selected for plant growth promotion or bioremediation capacity, its genome also encoded genes for root colonization, pyoverdine synthesis, oxidative stress (present in other soil isolates), degradation of aromatic compounds, heavy metal resistance and nicotinic acid degradation, manganese (Mn II) oxidation. Genes for toluene or naphthalene degradation found in the genomes of P. putida F1, DOT-T1E, and ND6 were absent in the P. putida LS46 genome. Heavy metal resistant genes encoded by the P. putida W619 genome were also not present in the P. putida LS46 genome. Despite the overall similarity among genome of P.putida strains isolated for different applications and from different geographical location a number of differences were observed in genome arrangement, occurrence of transposon, genomic islands and prophage. It appears that P.putida strains had a common ancestor and by acquiring some specific genes by horizontal gene transfer it differed from other related strains.
- Pseudomonas putida
- Comparative bioinformatics analysis
- Comparative genome analysis
- Polyhydroxyalkanoates
- Pan-Genome
- Insertion sequences
- Metabolic diversity
The genus Pseudomonas consists of a very heterogeneous group of microorganisms isolated from diverse environments and belonging to the gamma (γ)-proteobacteria (Palleroni [1984]). The genus has approximately 100 named species grouped into different subgroups based on multi-locus analysis (Winsor et al. [2011]). The genus Pseudomonas contains human pathogens, plant pathogens, and heterotrophic bacteria prevalent in soil, water, and on plant surfaces. Most pseudomonads are free-living saprophytic organisms in soil or water. Because of their great metabolic diversity they play an important role in decomposition, biodegradation, and the carbon and nitrogen cycles. Pseudomonas putida is non-pathogenic soil bacteria and is capable of degrading xenobiotic and promoting plant growth after root colonization as well as simultaneously providing protection for the plant from pests and other harmful bacteria (Compant et al. [2005]; Singleton [1994]).
Pseudomonas putida KT2440, P. putida BIRD1, P. putida UW4, P. putida S11 were isolated from rhizosphere soil and developed as plant growth promoting rhizobacteria (Glick [1995]; Matilla et al. [2011]; Nakazawa [2002]; Ponraj et al. [2012]). Pseudomonas putida F1 was isolated from polluted soil and developed as a bioremediation agent (Choi et al. [2003]; Eaton [1997]). Pseudomonas putida S16 was isolated in China and identified as a nicotine degrading strain (Tang et al. [2012]). Pseudomonas putida ND6 was identified as a naphthalene degrading bacterium, in which naphthalene degradation genes were located on a plasmid (Dennis et al. [2004]; Li et al. [2002]). Pseudomonas putida DOT-T1E was isolated as a toluene tolerant bacterium (Ramos et al. [1995]), while P. putida W619 was isolated as an endophyte of poplar (Taghavi et al. [2005]). Pseudomonas putida GB-1 was isolated from fresh water and identified as manganese oxidizer (Rosson and Nelson [1982]). None of P.putida strain was isolated for PHAs production; however, P.putida KT2440 was reported as a PHAs producer (Haywood et al. [1989]; Huisman et al. [1989]).
Polyhydroxyalkanoates (PHAs) are the largest group of biologically produced biodegradable biopolyesters providing different properties and applications (Huijbert and Eggink [1996]; de Smet et al. [1983]). A wide variety of microorganisms accumulate PHAs as a carbon and energy storage material in carbon-excess conditions when a major nutrient (typically nitrogen or phosphorus) is limiting (Elbahloul and Steinbüchel [2009]; Rehm [2010]; Brandl et al. [1988]). Accumulation of excess carbon is a general mechanism used by Pseudomonas and is essential for resource balancing (de Eugenio et al. [2010a]; Escapa et al. [2012]). De Smet et al. ([1983]) detected the inclusion bodies in Pseudomonas olevorans for first time when grown on octane and identified is as polymer of 3-hydroxyoctanoate. Huisman et al. ([1989]) studied the PHAs synthesis in fluorescent pseudomonads and confirmed that P.putida, P.fluorescens, P.aeruginosa, P.tetosteroni and P. oleovorans have the capacity to produce PHAs. Among the P.putida strains only P.putida KT2440, which was isolated as a plant growth promoting bacterium, has been studied for PHAs production.
Pseudomonas putida LS46 was isolated from wastewater on the basis of its ability to synthesize novel medium chain-length polyhydroxyalkanoates (mcl-PHAs), which accumulated 22% - 56% of cell dry weight when cultured, under batch culture conditions in media containing glucose, waste vegetable fryer oils or fatty acids (Sharma et al. [2011]). Analysis of P. putida LS46 16S rDNA displayed more than 99% nucleotide sequence identity to other P. putida strains. Complete genome sequence of other P.putida strains which were developed for plant growth promotion or degradation of xenobiotic, are available. Last genome comparison of P. putida strain W619 identified genes for aromatic pathways, heavy metal resistance and plant growth promotion in P.putida strain KT2440, F1 and GB-1 (Wu et al. [2011]). Since then six new P. putida strains, including P. putida LS46 genomes have been added to the database. The objective of this work is to see how the genomes of P.putida identified as either as biocontrol or bioremediation agents differ from each other due to their geographic origin, environment. Keeping this in mind the genome sequence of P. putida LS46 was compared with the genomes of nine other P. putida strains for general genome features, shared gene analysis, occurrence of insertion sequences, prophages, genomic islands and for metabolic diversity including PHAs production. The present study revealed more similarity than variability among the genome of P. putida strains isolated from different ecological niches in different locations.
Materials and methods
Genome sequences and accession numbers
P putida LS46 was isolated from a municipal wastewater treatment plant in Winnipeg, Manitoba, Canada, and the strain was deposited with International Depository Authority of Canada (IDAC) at the National Microbiology Laboratory, Health Canada Culture Collection (NML-HCCC), WDCM number 840 (Sharma et al. [2011]). The P. putida LS46 genome was sequenced by the Genome Canada sequencing facility at McGill University, Montreal, Quebec, using a combination of Illumina Gaii (Bennett [2002]) and 454 pyro-sequencing technology (Margulies et al. [2005]). The draft genome sequence of P.putida LS46 was deposited in the NCBI GenBank database with Accession number ALPV02000000 (Sharma et al. [2013]). The complete genomes of nine P. putida strains KT2440 (NC002947), F1 (NC009512), BIRD1 (NC017530), GB-1 (NC010322) S16 (CP003734), ND6 (CP_003589), DOT-T1E (NC_018222), UW4 (CP_003890) and W619 (NC010501) were obtained from the Joint Genome Institute database (http://img.jgi.doe.gov) and compared with the newly sequenced P. putida LS46. General genome features like genome size, total number of genes, number of coding sequences (CDS), number of Clusters of Orthologous Groups (COGs) were compared among the genomes of the 10 P. putida strains.
Phylogenetic relationship among Pseudomonas strains
The P. putida LS46 chaparonin gene (cpn 60) universal target (UT) sequence was obtained from the P. putida LS46 genome sequence and aligned with the cpn 60 UT sequences of 29 Pseudomonas strains available in the Integrated Microbial Genome database Markowitz et al. [2006]). The sequences were aligned using Bioedit (Thompson et al. [1994]) and phylogenetic analysis was conducted using MEGA5 (Tamura et al. [2011]). Phylogenetic tree was constructed using the neighbor-joining method (Saitou and Nei [1987]).
Occurrence of prophage, insertion sequence and genomic islands
The ten P. putida genomes were analyzed for presence of prophages using the PHAST (PHAge Search Tool) prophage finder database (Zhou et al. [2011]). Insertion Sequence (IS) elements in the P. putida genomes were identified using the ISfinder tool (Siguier et al. [2006]; Varani et al. [2011]). Genomic islands in P.putida genomes were identified using Island finder web based tools (Langille and Brinkman [2009]).
Whole genome alignments, BLASTn and Pan-genome analysis
Whole genome identity was calculated from pair-wise comparison of genomes using BLASTn analysis. Comparative synteny Dot plot analysis of the P. putida strains was carried out and nine plots of comparison between P. putida LS46 and nine P. putida strains were obtained (Huang and Zhang [2004]). Maps showing P. putida strains were prepared using the Gview server P. putida LS46 was compared with the other eight P. putida strains using Pan genome analysis from Gview server (Petkau et al. [2010]). Shared genes in different strains were calculated using the Phylogenetic profile program using a single gene profile from the Integrated Microbial Genome server. The number of homologous genes in genome 1 in comparison to genome 2 was counted. Reciprocally, the numbers of homologous genes in genome 2 in comparison to genome 1 were counted. Percent shared genes were calculated by adding the homologous genes in genome 1 and 2, dividing by the total number of genes in genome 1 + 2, and multiplied by 100 (Palmer et al. [2012]).
Comparison of metabolic genes
Genes related with different metabolic functions were identified by searching for homologous genes in P.putida genomes by using BLAST analysis of find gene in IMG web page using default parameter (Integrated Microbial Genome). Homologous genes for aromatic compound degradation, heavy metal resistance, manganese oxidation, nicotinic acid degradation, iron scavenging were identified using BLASTn analysis of IMG web page. Likewise TonB dependent receptor and dioxygenase genes were identified in different P.putida strains.
Phylogenetic relationship of P. putida LS46 to other Pseudomonas strains
Genome features of P. putida LS46
General features of the genomes of P. putida strains
Isolated in
Genome size
Gene count
Homologous genes (%)a
% Homologyb
CDS count
16S rRNA
COG count
Protein W FPc
Protein w/o FP d
Matilla et al. [2011]
Udaondo et al. [2012]
Wu et al. [2011]
Wu et al. [2011]
Nelson et al. [2002]
Sharma et al. [2013]
Li et al. [2012]
Yu et al. [2011]
Duan t et al. [2013]
Wu et al. [2011]
Insertion Sequence in P. putida LS46
Blast analyses of the P. putida LS46 genome with IS finder database identified three complete (ISPa41, ISPs1, ISPa42) and one partial (ISPpu9) IS elements (Additional file 1: Table S1). IS element ISPa41, which belongs to IS5 family, was earlier identified in P. aerugonosa DK2, P. putida, and P. syringae DC3000 (IS finder database). Blasting of ISPa41 sequence of P.putida LS46 identified homologous sequences in P.putida strain GB-1, KT2440 and ND6 but not in P.putida BIRD1, DOT-T1E and UW4. Partial sequences of ISPa41 were also present in P.putida F1, S16 and W619. ISPs1 was originated from P. syringae pv. syringae plasmid pPSR1 and P. syringae pv. savastanoi. ISPs1 from P. putida LS46 had 84% nucleotide sequence identity to ISPs1 from P. syringae plasmid pSR1. Complete ISPsI was also present in P.putida GB-1 and ND6. Incomplete ISPsI was also detected in P.putida strain S16, F1 and W619. Complete ISPpu9 from P. putida KT2440 was 2043 bp but in P.putida LS46 partial ISPpu9 was present (1697bp). It showed 98% sequence identity to ISPpu9 from P. putida KT2440. The ISPpu9 integration target site is 23 bp sequence, which is a part of REP identified in P. putida KT2440 (Aranda-Olmedo et al. [2002]). ISPpu9 was also present in P.putida BIRD1 but was absent in P.putida F1, GB1, DOT, ND6 UW4 and W619.
ISPa42, which is synonymous to ISPsy10, encodes a Tn3-like transposable element, which is thought to originate from P. aeruginosa DK3. ISPa42, a complete Tn 3 element of 16941 bp in P. aeruginosa DK2, was detected in P. putida LS46, but was disrupted by an intergenic region of 19922 bp. It bifurcated it into two segments of 4765 and 12176 bp. This 17 kb transposon was a deletion derivative of Tn4661 toluene degradation transposon Tn4651. The 19.9kb inergenic region of Tn3 carried heavy metal resistance genes like czcA efflux protein (PPUTLS46_008564) copA (PPUTLS46_008564), copB (PPUTLS46_08569), copper translocating P-type ATPase (PPUTLS46_008644), blue copper domain (PPUTLS46_008654), two component metal regulator sensor signal (PPUTLS46_8669) and heavy metal sensor signal transduction histidine kinase (PPUTLS46_008674) along with sterol desaturase (PPUTLS46_008539). This transposon was unique and was not present in any other P.putida strains. Tn4651, a toluene transposon, carried genes for toluene degradation (xyl) genes (Tsuda et al. [1989]). P.putida LS46 did not have toluene degradation ability due to deletion of toluene genes in Tn4661. A number of catabolic transposons have been identified in Pseudomonas species for toluene, naphthalene, chlorobenzoate, chlorobenzene and halogenated alkanoates (Wyndham et al. [1994]). Absence of these catabolic transposons in P.putida LS46 limited it metabolic diversity.
Prediction of prophages
Prophage finder (PHAST) identified one intact prophage and one putative phage genes in P. putida LS46 genome. The intact prophage sequence was was present on Contig ALPV02000012 (18373 to 68052) and was 49.6 kb in size with G + C ratio of 59.64%. A total of 61 CDSs which included 40 phage proteins, 21 hypothetical proteins and one tRNA were identified in this prophage. P.putida LS46 prophage showed upto to 73% nucleotide sequence identity to Pseudomonas aeruginosa phage Pseudomonas vB_PaeS_PMG1 which is a virulent phage with lytic infection cycle (Krylov et al. [2012]). A 79 bp attachment sites (attL and attR) were also present. This phage was also present in P.putida BIRD1, P.putida KT2440 and P.putida W619 genomes (Additional file 1: Table S2). However the possible prophages are decided on the basis of number of CDS shared with other prophages. P.putida LS46 shared 15 CDS with Pseudomonas vB_PaeS_PMG1 while P.putida BIRD1, P.putida KT2440 and P.putida W619 had 8, 14 and 15 CDS respectively. The organization of prophage Pseudomonas vB_PaeS_PMG1 genes was unique to P.putida LS46. The putative prophage was only 8.4 kb and had only 9 protein coding sequences. It was similar to SXt2_1717. Other P. putida genomes (from PHAST database http://phast.wishartlab.com) were predicted to contain 1–6 (intact, incomplete, and questionable) prophages (Additional file 1: Table S2). No prophage was detected in P. putida UW4.
Genomic islands in P.putida LS46
Comparative genome analysis of P.putida strains
The genomes of nine P. putida strains range in size from 5.73 to 6.3 Mb, with an average GC content of 62% (Table 1). Among P. putida strains only strain ND6 carried two plasmids of 117 kb and 101 kb, which encoded an additional 136 and 102 genes, respectively (Table 1). P. putida strains KT2440, BIRD1, GB1, and LS46 had seven copies of 16S rRNA genes, while six copies of 16S rRNA genes were present in strains F1, ND6, UW4, and S16 (Table 1). P. putida strains carried 4171 to 4519 COG counts, representing 69.7% to 82.0% of their genes.
Percent shared genes among different Pseudomonas putida strains
Genome arrangement of P. putida genomes
Homologous and shared gene among P.putida strains
House keeping genes
The 16S rDNA is an universal marker and has been widely used for comparison between divergent bacteria. However, the resolution of 16S rRNA gene sequences at the intrageneric level is low Anzai et al. [2000]; Yamamoto et al. [2000]). Gene sequences for 'housekeeping' proteins provide better phylogenetic resolution and have been used to differentiate the genomes of closely related strains. Zeigler ([2005]) identified some house keeping genes for studying genome relatedness among different strains. Comparison of 33 house keeping genes of P. putida LS46 with other nine P.putida strains revealed a high degree of homology among these genes (Additional file 1: Table S4). However, some house keeping genes like dnaX (PPUTLS46_019951), lig (PPUTLS46_022046), pgi (PPUTLS46_007236), uvrC (PPUTLS46_012340,) glyA (PPUTLS46_024688), trpS (PPUTLS46_013403) and trmE (PPUTLS46_016619) were highly diverse among P. putida strains (Additional file 1: Table S4).
Fatty acid and Polyhydroxyalkanoate synthesis
Homology of proteins associated with polyhydroxyalkanoate production
Locus tag
Product size (aa)
Acyl-CoA synthetase
3-ketoacyl-CoA thiolase
Acetyl-CoA acetyltransferases
3-hydroxyacyl-CoA dehydrogenase/epimerase
3-hydroxyacyl-CoA dehydrogenase
Acyl-CoA synthetase
Acyl CoA dehydrogenase
Acyl CoA dehydrogenase
Poly(R)-hydroxyalkanoic acid synthase
Poly(3-hydroxyalkanoate) depolymerase
Poly(R)-hydroxyalkanoic acid synthase
Transcriptional regulator
PHA granule associated protein
PHA granule associated protein
Hydroxyacyl-ACP:CoA transacylase
malonyl CoA-acyl carrier protein transacylase
3 hydroxyacyl(decanyl)-ACP dehydratase
2 -Oxoacyl carrier protein synthase
3-ketoacyl-(acyl-carrier-protein) reductase
3-oxoacyl-(acyl-carrier-protein) reductase
3-oxoacyl-(acyl-carrier-protein) reductase
3-hydroxyacyl-[acyl carrier protein] dehydratase
Comparison of common metabolic pathways of P. putida strains
Metabolic diversity
The compared P. putida strains were isolated from diverse environment for bioremediation of xenobiotics or decomposition of other materials. Different P. putida strains have unique genes associated with specific functions and these genes are not present in other strains. P. putida KT2440 has been considered as a metabolically diverse saprophytic bacterium. Analysis of the P. putida KT2440 genome identified 18 dioxygenase, 51 putative hydrolases, 40 dehydrogenases, and more than 62 transferases (Jimenez et al. [2002]; Molina-Henares et al. [2009]; Nelson et al. [2002]). By way of comparison, the P. putida LS46 genome had 22 dioxygenases (Additional file 1: Table S5), 75 hydrolases, 149 dehydrogenases, and 43 transferases. All the three dioxygenases, benzoate dioxygenase (PPUTLS46_007854, PPUTLS46_007859), catechol dioxygenase PPUTLS46_007879), and protochatuate dioxygenase (PPUTLS46_010694) were present in P. putida LS46. These dioxygenases were earlier identified in P.putida KT2440 and are involved in metabolism of aromatic compounds (Nelson et al. [2002]). P.putida LS46 had 22 dioxygenases genes. One of the dioxygenase genes (PPUTLS46_007879 glyoxalase/bleomycin resistance protein/dioxygenase) was unique to P.putida LS46 and was not present in other P.putida strains (Additional file 1: Table S5).
The majority of the genes for degradation of ferulate, coniferyl, and coumuryl alcohol, aldehydes and acids, p-hydroxybenzoate, and protocatechuate present in P. putida KT2440 were also present in P. putida LS46 (Additional file 1: Table S6). These genes were reported to have role in degradation of aromatic/aliphatic sulphonate, benzoate, toulate, catechol, hippurate, maleate, phenylalanine, phenylacetic acidprotocatechuate, quinate, taurine etc. The notable exceptions in P. putida LS46, however, were ferulic acid transferase (PP_3354), transferuloyl CoA hydratase (PP_3358), and vanillate dehydrogenase (PP_3357). These genes were present in P. putida F1 (Pput_2404, Pput_2400, Pput_2401) and P. putida W619 (PputW619_2051, PputW619_2047, PputW619_2048), and are associated with transformations of ferulic acid to vanillin and vanillin to protocatechuate. Another gene, which was only reported in P. putida KT2440, and that is missing from all other P. putida strains, was propanediol dehydrogenase (PP_2803).
P. putida F1 is another metabolically diverse strain that has been demonstrated to oxidize toluene, 3-hydroxyphenyl propionate, and cymene (Finette and Gibson [1988]; Zylstra et al. [1988]). The genes that encode the enzymes for these reactions were also present in P. putida DOT-T1E, which encodes the genes (TIE_4277-T1E-4240) for degradation of toluene, 3- hydroxyphenylpropoinate, and cymene (Additional file 1: Table S8), but absent in P. putida LS46 and 7 other P. putida strains. As reported earlier the genes for degradation of 3 hydroxyphenylpropionate were also present in P.putida W619 (Wu et al. [2011]).
P. putida KT2440 can use aromatic or aliphatic sulphonates as sulphur sources. The genes for degradation of aromatic or aliphatic sulphonates are encoded in the ssuFBCDEA operon (PP_0241-PP_0235) in P. putida KT2440. This operon was present in P. putida LS46 (PPUTLS46_024748, PPUTLS4_025163, 025168, 025173, 025178, 025183) and all other P. putida strains (Additional file 1: Table S6). Three chlorohyrolases i.e. atrazine chlorohydrolase (PP5036, PP2584) and hydroxydechloroatrazine ethylaminohydrolase (PP3209) detected in P. putida KT2440 were also identified in P. putida LS46 (PPUTLS46_10994, PPUTLS46_005456, and PPUTLS46_008014). However, chloride channel protein of P. putida KT2440 (PP3959) was absent in P. putida LS46. Pak et al. ([2000]) identified xenobiotic reductase from P. fluorescens for the transformation of 2, 4, 6-trinitritoluene (TNT). Its homologue was identified in P. putida KT2440 (PP_0920) (Nelson et al. [2002]) and P. putida LS46 (PPUTLS46_022986). These genes was also present in other eight P. putida strains, but was absent from P. putida UW4.
Pseudomonas putida DOT-T1E is a solvent-tolerant strain able to grow in the presence of > 1% (v/v) toluene in the culture medium. Its multidrug efflux pumps have been found to play a major role in toluene-tolerance (Rojas et al. [2001]). This ability is mainly conferred by an efflux pump encoded in a self-transmissible 133 kb plasmid named pGRT1 (Molina et al. [2011]). No plasmid was detected in P. putida LS46, but the genes encoding an efflux pump (sepA, sepB, sepC), were present in the P. putida LS46 (PPUTLS46_0133778, 013773, 013768) genome, as reported earlier for P. putida strains KT2440, GB1 and W619 (Wu et al. [2011]).
Nicotinic acid and nicotine degradation
Nicotinic acid (NA) is a carboxylic derivative of pyridine that is widely distributed in nature as part of pyridine cofactors (NAD and NADP) and alkaloids (e.g., nicotine and anabasine), and it is essential (vitamin B3) for those organisms that are not able to carry out its synthesis. In bacteria and fungi, NA is used as a carbon source. The biochemical pathways involved in the degradation of NA was elucidated (Kaiser et al. [1996]). Jiménez et al. ([2008]) identified a nic gene cluster (PP_3939 - PP_3948) in P. putida KT2440 responsible for aerobic nicotine degradation. These genes were also present in P. putida strain F1, GB1, and W619 as reported earlier Jiménez et al. ([2008]). All the genes of nic operon were also present in P. putida LS46, BIRD1, ND6, DOT-T1E, and these genes were homologous to genes (89.6% to 100% nucleotide identity) from P. putida KT2440 (Additional file 1: Table S7).
Recently, the genome sequence of another nictotine degrading P. putida, strain S16, was released (Tang et al. [2012]). P. putida S16 degrades nicotine through pyrrolidine pathway. A gene cluster containing six genes encoding PPS_0380 HSP hydroxylase (hspB), PPS_4060 maleate isomerase (iso), PPS_4059 NFM deformylase (nfo), PPS_4058 DHP dioxygenase (hpo), PPS_4057 maleamate amidase (ami), and PPS_4061 6-hydroxynicotinate 3-monooxygenase (hna) was identified in P. putida S16 and this gene cluster is designated as nic 2 cluster. This gene cluster was present on a large genomic island. Both nicotine and nicotinic acid produced 2,5- dihydroxypyridine (2,5-DHP) as an intermediate, which is converted to N-formylmalaemic acid. The N-formylmalaemic acid is degraded to maleic acid and then to fumaric acid as in nicotinic degradation pathway (Wang et al. [2007]). BLAST analysis indicated that these genes were also present in P. putida LS46 (PPUTLS46_011665 (ami), 11670 (iso), 11675 (nfo), 11685 (hpo) and showed 36.63, 69.35, 55.64 43.73% homology to respective proteins of P.putida S16. Other P.putida strains showed low homology to the P. putida S16 nic 2 cluster (36.6-65-3%). No homologue of PPS_0380 HSP hydroxylase (hspB) was present in P.putida LS46 (Additional file 1: Table S8). The nic cluster genes of P. putida KT2440 showed high nucleotide sequence identity (>95%) to corresponding genes of P.putida LS46, as reported earlier in P. putida strain W619, F1, and GB1 (Molina et al. [2011]) [59]. P. putida KT2440, however, is not able to degrade nicotine. The nic cluster of P. putida S16 had a GC content of only 48% compared with the 62% GC content of the rest of the P. putida genome sequence. The nic gene cluster for the nicotinic acid degradation pathway of P. putida KT2440 was also detected in P.putida BIRD1, P. putida ND6, and P. putida DOT-T1E, but was absent in the genome of P.putida strain UW4.
Heavy metal resistance
The level of heavy metal tolerance is very high in P. putida W619 in comparison to other P. putida strains (Canovas et al. [2003]; Taghavi et al. [2009]). In P. putida W619, copper, cobalt, zinc, cadmium, arsenate, mercury, nickel chromate, and molybdenum resistance genes are present in two genomic regions (1 and 31). Heavy metal tolerance of P. putida LS46 has not been investigated, but this strain encodes genes for heavy metal resistance present in genomic region 1 of P. putida W619. These genes are involved in copper, cobalt, zinc, and cadmium resistance. However, the genes located on genomic region 31 of P. putida W619, involved in copper and chromate resistance, were absent in the majority of P. putida strains, including P. putida LS46 (Additional file 1: Table S8). All P. putida strains lacked the genes present in genomic region 18 of P. putida W619, which were associated with mercury and nickel resistance.
Manganese oxidation
P. putida GB1 is a Mn (II) oxidizing bacterium (Buzzo [2011]) and attempts have been made to identify the genes related with Mn (II) oxidation by transposon mutagenesis. However, the role of different genes in Mn (II) oxidation is not clear (Caspi et al. [1998]; Brouwers et al. [1999]). Recently, using transposon mutagenesis, Geszvain et al. ([2013]) and Geszvain and Tebo ([2009]) identified two genes, which are homologous to multi-copper oxidases (PputGB1_2447 and PputGB1_2665) in P. putida GB1. The genes encoding multi-copper oxidase homologues of P.putida GB1 were also present in P. putida LS46 (PPUTLS46_002532 and PPUTLS46_006964), as well as in P. putida strains KT2440, F1, ND6, W619, S16, UW4, and DOT-T1E. Only one multi-copper oxidase gene was identified in P. putida BIRD1 (Additional file 1: Table S8).
Iron scavenging genes in P. putida strains
It is well known that iron deficiency in bacteria limits growth Andrew et al. ([2003]). Pseudomonas produces low molecular weight iron-chelating compounds termed ferri-siderophores to scavenge iron (Wiener [2005]). Ferri-siderophores are taken up via outer membrane receptors, which function as gated porin channels (Koebnik [2005]; Ratledge and Dover [2000]). After binding of the ferri-siderophores, transport is mediated by a complex of inner membrane-anchored proteins TonB (Wiener [2005]). Fluorescent pseudomonads respond to iron-deficiency by secreting the yellow-green fluorescent peptidic siderophores called pyoverdines (Meyer [2000]; Cornelis et al. [2009]). Pyoverdines have a conserved chromophore with a variable peptide chain, and each Pseudomonas species produces a different pyoverdine (Ravel and Cornelis [2003]; Visca et al. [2007]). Pyoverdine is synthesized by non-ribosomal peptide synthetase (NRPS). Like other P. putida strains, genes for pyoverdine synthesis were also present in P. putida LS46 (PPUTLS46_025659 and PPUTLS46_012775). The pvdE gene (PPUTLS46_012775) encoded a PVD ABC transporter, as in other P. putida strains. P. putida LS46, like the other P.putida strains did not carry the genes for pseudomonine production, which was reported for P. entomophila L48 (Vodovar et al. [2006]).
The number and type of TonB siderophore receptors show their diversity to survive in the different environment (Cornelis and Bodilis [2009]). TonB-dependent receptor genes are not constitutively expressed and are regulated by the iron availability (Koebnik et al. [2000]; Bodilis et al. [2009]) and their expression provides additional mechanisms to survive under different niches. Twenty-six (26) TonB siderophore receptor genes were present in the P. putida LS46 genome (Additional file 1: Table S9). Across all of the P. putida strains, 19–53 TonB genes are present. Comparison of TonB genes (at 80% nucleotide sequence identity) of P. putida LS46 identified 26, 28, 26 20, 24, 26, 19, 1 and 15 homologous TonB genes in P. putida LS46 strain BIRD1, DOT-T1E, F1, GB1, KT2440, ND6, S16, UW4 and W619, respectively. P.putida strain UW4 had 11 TonB genes, which showed low homology (50% nuclotide sequence identity) to other TonB genes of P. putida strains. The greatest number of TonB genes (55) was present in P. putida strain GB1.
Pseudomonas putida strains have been identified as root colonizing bacterium and developed as biocontrol agent. Other P.putida strains due to their diverse metabolic potential have been developed as bioremediation agents. Potential of P.putida as polyhydroxyalkanoates (PHAs) producer was identified in 1989 (Huisman et al. [1989]). However, PHAs producing P.putida strain KT2440 was originally isolated as a root colonizing bacterium (Nelson et al. [2002]). A number of P.putida strains were isolated and developed as bioremediation agents but no other P.putida strain was isolated for PHAs production. P.putida LS46 was isolated and screened for PHAs production and it was proved as a good PHAs producer as P.putida KT2440. Complete genome of this bacterium was sequenced to know the similarities and differences among biocontrol, bioremediation and PHAs producing P.putida strains.
P. putida LS46 strain showed more than 99% identity to 16S rRNA gene of P.putida strains irrespective of their geographic origin or their application. P.putida classifications based on protein-encoding genes like gyrB, rec, rpoB, recN, and cpn60 alongwith 16S rRNA gene have earlier been proposed (Adékambi et al. [2009]; Arahal et al. [2008]; Zeigler [2005]; Mulet et al. [2010, 2013]). Cpn60 (Hsp60 or GroEL), a highly conserved protein found in bacteria, has been widely used for phylogeny, microbial identification, microbial ecology and evolution (Hill et al. [2002, 2004]). The phylogeny of P. putida strains based on cpn60 confirmed the close relationship among P. putida strains, strengthening the 16S rRNA gene based phylogeny. However, the cpn60 gene phylogeny was more robust and separated strains that were clustered together in the 16S rRNA gene phylogeny. The cpn60 (UT) analysis differentiated P. putida W619, P. putida S16, and P. putida UW4 from other P. putida strains. P. putida W619 was isolated as an endophyte of Poplar, P. putida UW4 was isolated as a plant growth promoting species, and P. putida S16 was isolated as a nicotine degrading species. Recently cpn60 (UT) was used for studying the phylogenetic relationship among Aeromonas, Themoanaerobacter, and vaginal microbiota (Miñana-Galbis et al. [2009], Verbeke et al. [2011]; Schellenberg et al. [2011]).
The phylogeny of P. putida UW4 was described on the basis of 16S rRNA genes and a multilocus approach that used four concatenated housekeeping genes (16S rRNA, gyrB, rpoD and rpoB). P. putida UW4 was found to be closely related to P. fluorescens rather P. putida strains including P. putida LS46 (Duan et al. [2013]). It confirmed our observations on the phylogeny of P. putida UW4 based on the cpn60 gene that P. putida UW4 was clustered with P. fluorescens. Earlier, Loper et al. ([2012]) on the basis of ten concatenated house keeping genes, showed similar phylogenetic relationships among Pseudomonas species. The cpn60 gene phylogeny placed the ten sequenced P. putida strains and P. entomophila in the same clade. The phylogeny of the newly isolated P. putida LS46 based on cpn60 differentiated this strain from other closely related P.putida strains, and based on both the 16S rRNA and cpn60 analyses, P. putida LS46 was closely related to type strain P. putida KT2440. No plasmids have been detected in P. putida LS46, but two plasmids were present in P. putida ND6 and one plasmid was identified in P. putida DOT-T1E (Li et al. [2002]; Udaondo et al. [2012]).
Variation in genome content is thought to be a key factor in the evolution of bacteria and variation in genome arrangement may also improve the fitness of the bacteria (Silby et al. [2011a, b]). Presence of duplicated genes like rRNA operons, multiple transposons, insertion sequences, prophages and genomic islands leads to genome rearrangements in bacteria that contribute to evolution (Tillier and Collins [2000]; Klockgether et al. [2010]). Rearrangements in genome are not random, but predominantly occur at end-points either at the origin or at the terminus of replication (Eisen et al. [2000]).
Overall, strains of P. putida isolated from different geographical regions and from varied ecological niches had high similarity in genome structure and functions (Molina et al. [2011]; Li et al. [2012]; Nelson et al. [2002]; Wu et al. [2011]). All the P. putida genomes had higher level of genome similarity, but differed from other on the basis of insertion sequence, presence of prophage and genomic islands. However, Dot plot analyses of the P. putida strain genomes identified a number of rearrangements, which are possibly due to presence of these genetic elements, such as the presence of ISPa42 in P. putida LS46, which originated from P. aeruginosa DK3. ISPa42 may be associated with the acquisition of genes for heavy metal resistance.
In comparison to P. putida LS46, genome inversions were observed in P. putida BIRD-1 and P. putida W619 at the origin and terminus of replication in P. putida S16 and P. putida ND6, respectively. These observations strongly support the contention that bacterial genomes are not static and significant variations are observed even among strains within the same species. These variations are the result of genome deletions and/or gene acquisitions by horizontal gene transfer of elements such as transposon and genomic islands (Hacker and Carniel [2001], Mackiewicz et al. [2001]).
P. putida LS46 and P. putida DOT-T1E had a maximum sequence identity of 97.7%, but their genome arrangements were very diverse (Figure 2). It appears that the genome of P. putida DOT-T1E has undergone a number of rearrangements without losing any major function. P. putida DOT-T1E was isolated as toluene degrading species, and has acquired genes for toluene degradation that are absent in P. putida LS46. Using BLAST analysis 81.84-93.99% genes were identified which were shared among the 10 P. putida strains, while the rest of the genes were unique to the strain. Ballerstedt et al. ([2007]), using specific gene probe of P. putida KT2440 in a microarray identified 67.8-100% identical genes in six P. putida strains. Acquisition of specific groups of genes conferred specialized functions to specific strains, but their core genomes were identical.
Three P. putida strains, KT2440, BIRD1, and UW4, were isolated from soil and developed as plant growth promoting bacteria. P. putida strains F1, ND6, DOT-T1E, S16, GB1, and W619 were identified as bioremediation agents on the basis of their capacity to degrade pollutants. P. putida strain LS46 was isolated from wastewater for PHA production. This strain was able to use glucose, glycerol, fatty acids, and waste fryer oil and accumulates PHAs to 20-58% of the cell dry weight. Like other P. putida strains, central metabolic pathways for utilization of these substrates were also present in P. putida LS46. The application based classification of P. putida as biocontrol, bioremediation, or PHA producer is not relevant because biocontrol agent BIRD-1, or bioremediation strains F1, ND6, and DOT-T1E also encode the genes for PHA synthesis and homology among these genes is very high. The most of the genes present in the manganese oxidizing strain P. putida GB-1 or the endophyte of poplar stain P. putida W619 are also present in P. putida LS46.
Complete genome sequence of P. putida LS46 was compared with P. putida strains isolated from different geographical regions and different niches. Two strains, P. putida ND6 and P. putida DOT-T1E isolated from China, and P. putida F1 isolated from USA, had the highest sequence identities to P. putida LS46, which was isolated in Canada. P. putida strains GB-1 isolated from USA, UW4 isolated in Canada, and S16 isolated from China, had low sequence identities with P. putida LS46. P. putida W619, which was isolated as an endophyte of poplar had only 87.1% sequence identity with the P. putida LS46 genome, while P. putida UW4 showed only 82.0% identity with P. putida LS46. Our earlier result on phylogeny of P.putida UW4 has clearly demonstrated that this strain is related to P. fluorescens rather than P. putida. The low genome identity of P. putida strains with P. putida UW4 is not surprising. High genome similarities among P. putida strains isolated from different regions indicate their common ancestry (Biello [2008]).
P. putida LS46 does not match, however, the metabolic diversity of either P. putida F1 or P. putida DOT-T1E, which encode genes for degradation of toluene, cymene, and 3-hydroxyphenylpropionate, or P. putida ND6, which encodes genes for naphthalene degradation. These genes may have been acquired during the evolution process. A number of transposons and insertion sequences were present in P. putida genomes indicating their labile nature. ISPpu10, a transposon present in REP sequence of P. putida KT2440 has been implicated in genome rearrangement (Ramos-Gonzalez et al. [2006]). But this transposon was absent from all P. putida strain except P. putida S16. Mg (II) oxidation (P. putida GB1), pyoverdine production, aromatic/aliphatic sulphonate degradation (P. putida KT2440), and polyhydroxyalkanoate production (P. putida strains KT2440 and LS46) are core genes that are present in all P. putida strains whether these strains were isolated as biocontrol agents or as bioremediation agents (Wu et al. [2011]). The genome of P. putida LS46 had a majority of the genes involved in plant growth promotion even it was not isolated as plant growth promoter and in this regards it was similar to P. putida KT2440 and P. putida BIRD1. Several unique genes were identified in the P. putida LS46 genome. The presence of prophages, insertion sequences, and genomic islands in different genomes indicate that the P. putida genome is not static, but changes by acquiring new genes from related and unrelated species.
Additional file
This work was supported by funds provided by the Natural Sciences and Engineering Research Council of Canada (NSERC), through a Strategic Programs grant (STPGP 306944–04), by Genome Canada, through the Applied Genomics Research in Bioproducts or Crops (ABC) program for the grant titled, "Microbial Genomics for Biofuels and Co-Products from Biorefining Processes", and by the Province of Manitoba, through the Manitoba Research Innovation Fund (MRIF).
Authors' Affiliations
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subset: commoncrawl


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A Flowered Tote Bag
Last year I'd picked up some fabric so I could make this tote bag. It took me until this year to get around to making it. It's a pretty simple pattern, but then I forget how slow I can be at sewing things. Glad to have it completed.


text: Roman Bisellium Stool Small 320×226 Medium 640×451 Large 1024×722 Download TIFF Original 2400×1692 | (362.6 KB) Description The Roman Bisellium Stool also called the Roman double stool, was made of bronze but did not have a seat covering. Its origin was Pompeii. Keywords bronze, double-stool, pompeii, Roman, Rome bisellium, stool Galleries Stools Source: Meyer, Franz Sales Handbook of Ornament (New York, NY: The Bruno Hessling Company, 1904)


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Watched episode 1 of Season 4 last night and though the episode was slow to start I was overall impressed with the storyline. Most of the regular characters in Bon Temps were shown and their stories updated, and few new characters were introduced.
I can't wait for next week's episode and maybe I'll grab a couple of bottles of Tru Blood to slip on during the show. I found a store in the local mall that sells them and I'm dying to try them.
Check out the video above for a preview of next's week episode.


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Music of Tuesday, 6 November 2012
Source: Arnold Asamoah-Baidoo
I don't espouse break-ups in music groups but I have also realized that it is an inevitable phenomenon that transpires within the entertainment industry.
Some break-ups are for good reasons and others are obviously necessitated by silly reasons. Break-up of music groups is not new, dating way back, way before Ike and Tina Turner broke up, before Michael Jackson separated from The Jackson 5 and way before the Lumba Brothers went their separate ways.
One observation I have made concerning break-ups in Ghanaian music is the fact that they are less tumultuous: regardless of the reasons for the break-up which varies from clash of ideas to disagreements on money – the group members keep the reasons for the break-up on the quiet and move on.
Praye's break-up came as a shock; it made the headlines and served as a topic for jousting on most entertainment discussion platforms. After a year or more after the break-up, you expect these guys to have moved on with their lives and respective careers, but no, they still talk about the break-up, assigning the same old reasons for the rift – and it is becoming a nuisance. Are you guys the first group to break up? Give us a break!
VIP broke up on the quiet, so did Lifeline Family, Sass Squad, Nananom, TH4Kwagees, Buk Bak, Akyeame and Wutah all did same, so members of Praye should please hush it and move on.
The aftermath of group break-up is the same; the first scenario is that, the artiste who breaks away from the group makes it big as a solo artiste like Lionel Richie from The Commodores, Diana Ross from The Supremes, Michael Jackson from the Jackson 5, Robbie Williams from Take That, Justin Timberlake from N'Sync, Beyonce from Destiny's Child, Bobby Brown from New Edition, 2Face from Plantashun Boys, 2Chainz from Playaz Circle and T-Blaze from Lifeline Family.
The second scenario is that, the remaining members of the group triumph with the group whiles the break-way artiste slumps like VIP and Friction, Brian McFadden and Westlife, Latoya and Latavia and Destiny's Child, David Ruffin and The Temptations.
The third is that, most members of the group go on to have hit songs like Daddy Lumba and Nana Acheampong from Lumba Brothers, Beyonce, Kelly and Michelle from Destiny's Child, Sydney and Omanhene Pozo from Nananom, Wyclef and Lauryn Hill from Fugees, Dr. Dre and Ice Cube from NWA.
The last scenarios that pertains after a group break-up is that, all members of the group struggle with their solo joints, like Layzie Bone, Krayzie Bone and Bizzy Bone, all from Bone Thugs-N-Harmony, like Bright and Ronny who came out with lopsided solo albums after the first Buk Bak severance, as well as Fiifi Sela and Atsu Koliko from TH4Kwagees. What about Afriyie and Kobby from Wutah? They seem to be floundering with their solo careers if you ask me.
With the four scenarios mentioned, members of Praye should realize that they will fall in any of the categories depending on the direction their respective careers go and they should also know that music enthusiasts are tired of hearing about their rift, on who said what or who did what and the cacophony that goes with it. We've had enough!
I love 'beef', a violence-free one because 'beef' gives opportunity to the artiste to express his angst and frustrations on wax, on a record without holding punches. It's the best way to settle scores for artistes to do all the 'dissing' in a song. If Praye Tintin is still agitated over the break-up and feels he was treated unfairly, he should do what every sane rapper will do.
He should stop wasting our time casting vituperations at his ex-group members and quit the 'girly' attitude of threatening harmless female presenters. He should rather enter the studio, get a banging production and pour his grievances on the song, 'diss' all of them and put it out there – finito!
My advice to the two remaining members is simple; you guys are prepping an album with the release of a new single. Leave issues on the break-up and concentrate on promoting the single and the upcoming album. Questions on the break-up will always come up whenever you guys mount any platform to promote your song, but it behooves you to respectfully shirk such questions.
To Praye Tintin: My brother, you are making too much jarring noise about this break-up. If you claim you were a pivotal member of the group who wrote most of the songs and contributed enormously to the success of the group, then prove them wrong and yourself right.
Go out there; soak yourself into making a hit song, feature one or two good singers and you are on to becoming a Michael Jackson or Lionel Richie. Stop calling into programmes or answering calls on radio and TV where your ex-group members are granting interviews. It is glaring that those guys don't want you, so my brother, forget them and surge on.
It is sad that a group like Praye, considering the cohesion, understanding, respect and camaraderie that existed between them, that saw them annex so many awards – could not solve their petty squabbles like it prevails in many groups and I do understand when some TV and radio presenters try to create some avenue for them to settle their differences. But it is enough; these guys are grown men with grown hairs all over their bodies and they should know what is right and wrong for them. Leave them alone!
To all the feuding members: You guys should realize by now that, what matters is good music which translates to airplay and then performances. These infantile bickering will not produce hits, unless maybe, you put it on a record. Concentrate on your respective careers, give it your all, get the necessary support and stop the noise-making on the break-up.


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First published in the Guardian on 25 August, 2016
Stravinsky: Petrushka, Symphonies of Wind Instruments, Orpheus
LPO/Jurowski (LPO)
Vladimir Jurowski has been principal conductor of the London Philharmonic Orchestra since 2007 and what comes across brilliantly on this new Stravinsky disc — recorded live in concert in 2014 and 2015 — is how focused and un-faffy he and the orchestra sound together by now. The playing is bright, focused, elegant, occasionally too much so. Petrushka (original 1911 version) is short on crazed energy and urban hubbub, but instead we get chamber-like clarity and a really crisp sense of the score's architecture. Orpheus has a sombre, stately beauty, and the Symphonies of Winds is performed in the original version with alto flute and alto clarinet giving excitingly mellow, gooey textures. Stravinsky described the piece as "an austere ritual" but also dedicated it to Debussy, and this performance clinches that balance between solemn observance and splendid colours. Best of all are the closing chorales, full of quiet, attentive poise.