text:
Financial services unit of a major business conglomerate
Financial services
Business need:
Consolidate operations and streamline invoice-to-cash function across 25 countries to improve effectiveness and efficiency
Genpact solution:
- Improved cash flows for P&L impact of $650,000
- Unapplied cash decreased from $80 million to just $1.7 million, creating a total P&L impact of $250,000
- Past-due accounts declined from $55 million to $35 million, with a resulting benefit of $300,000
- Total operational cost savings were $1.3 million per year
Business impact:
- Growth and scalability
- Adaptation and flexibility
- Cash flow and liquidity
Business challenge
Aiming to improve cash flow and liquidity, the financial services unit of a major business conglomerate turned to Genpact to consolidate the company's operations. The focus was managing the invoice-to-cash cycle, including collections and cash applications. An accounts receivable team in the Netherlands managed this process, covering a multi-country operational and client base across the European Union, the United Kingdom, and the United States.
By streamlining processes, the organization sought to reduce operational costs and improve efficiency and effectiveness. A related goal was to consolidate the operational structure with a scalable operational model for greater agility in a dynamic business environment.
Genpact approach
Working with the accounts receivable team, Genpact developed and initiated a transformation project for invoice-to-cash operations. The project applied a hybrid model to draw upon synergies within the Genpact skills base and the client organization.
The Genpact-led team created an enterprise process for collections and cash applications across geographic units. With improved operating margins, the initial target was $2 million in savings from improvements in:
- Quality: 1.5x improvement in "First Time Right" process accuracy
- Speed: 1.8x improvement in the cycle time for collections and cash reconciliations
- Cost: a 40% decrease in operational costs
The scope of the business included serviced assets of $30 billion across 25 countries. Genpact's implementation of policies, standards, and technology was a transformative program that covered the entire asset base and operational scope. The program accounted for the unique regulatory environment in each geographic area.
Genpact solution
Genpact applied its rigorous Six Sigma framework and governance structure to deliver a total business impact of $2.5 million, using a four-tier approach.
1. Creating the ecosystem
The Genpact team began the transformation began by creating the ecosystem—a Genpact framework for process excellence—and impact levers (see Figure 1).
The team created a diagnostic process health framework to reduce bottlenecks. It included detailed value-stream mapping of each process, along with links to upstream and downstream activities. Genpact used its Six Sigma and Lean methodology to improve various auxiliary processes and create administrative changes.
An end-to-end study of the business value chain, process gaps, and links identified opportunities for reengineering. These major Six Sigma black-belt efforts required upstream process redesign, including risk-scoring models and improvements in billing and invoicing processes.
Genpact created enterprise-level processes using global benchmarks, insights, and practices refined over 14 years of experience in the order-to-cash discipline. The team helped reorganize, redefine, and recalibrate the client's operating mechanisms and produce a best-in-class solution.
2. Project governance structure
To ensure a seamless flow of delivery objectives and communications, the team established a project governance structure. It encompassed all levels of employees as they engaged with stakeholders. The aim was to establish controllership without impacting the process metrics (see Figure 2).
- Operations – This element of the structure ties together operations managers from Genpact and the client to execute deliverables and measure improvement.
- Management – Operations leaders communicate the organizational goals and design of the project plans; the leaders also manage cultural changes.
- Decisions – Strategic business decisions are made at the CxO level for overall business strategy and change management.
3. Mapping process goals to desired project outcomes
Genpact helped create an accounts receivable model to standardize the overall process and measure each discrete step (see Figure 3). The model incorporated Six Sigma methodologies to map transformation projects to the process level. Key project outcome measures included:
- Cost to serve
- Reserve ratios
- Cash flow
- Days sales outstanding (DSO)
- Client satisfaction index
Process improvements were identified by establishing links among outcomes in the following areas:
- Underwriting cycle times
- Automation levels for underwriting and cash application
- Accuracy of transactions
- Administrative delinquency
- Invoicing dispute levels
4. Creating the business impact
The Genpact-led project realized a total annualized value of $2.5 million for the client in several key areas (see Figure 4):
- Quality – Unapplied cash decreased from $80 million to just $1.7 million, creating a total P&L impact of $250,000. Past-due accounts declined from $55 million to $35 million, with a resulting benefit of $300,000.
- Faster cash flow – Same-day cash recognition improved from 83% to 91%, which yielded a $250,000 business impact. Collection cycles decreased by five days, creating a total gain of $400,000.
- Cost of operations – With overall efficiency gains, cash operations headcount decreased by 42 FTEs through process-automation tools, workforce management, and level loading. Total operational cost savings amounted to $1.3 million per year.
- Best practices – Genpact helped create a sustainable accounts receivable model that can be replicated in any global accounting operation. Implementing the invoice-to-cash technology suite helped create an innovative Business Process as a Service (BPaaS) model for an accounts receivable operation (see Figure 5).
Business impact
The client made good use of Genpact's ability to apply business process management expertise and methodology in ways that create value beyond cost savings. The project delivered high-value financial outcomes through productivity gains with consolidated and streamlined operations.
For more information, contact: firstname.lastname@example.com and visit, genpact.com/what-we-do/industries/banking-financial-services, genpact.com/what-we-do/business-services/finance-accounting


text:
Unjustifiable/ unjust/ unfair/ wrongful dismissal is a term in the labour law to describe an act of employment termination or dismissal of an employee, without sound justifiable reasons, or contrary to the legislative laws of the country in which the company operates. Different countries have diverse laws in this regard, but more or less, all the laws tend to favour the employee and protect his interests. The obligation of justifying any dismissal generally lies with the employer.
Being terminated for reasons like discrimination on the basis of gender, race, religion, caste etc., or for filing a retaliation to discrimination, is treated unlawful. Similarly refusal to commit an illegal act or act not under the purview of the company, if leading to dismissal of an employee, is termed as illegal. The employer is expected to abide by the termination procedures as communicated to the employee under the contract of employment.
Employees may seek redressal under law if they perceive their termination to be illegal. The remedy, if the employee is proved right, may include reinstatement and/ or monetary compensation.
Hence, this concludes the definition of Unjustifiable dismissal along with its overview.
Browse the definition and meaning of more terms similar to Unjustifiable dismissal. The Management Dictionary covers over 7000 business concepts from 6 categories.
Search & Explore : Management Dictionary
Related Business Content
Top Companies Lists

subset: commoncrawl


text:
Augmented reality software refers back to the precise code and software that bridge the area between your real and digital worlds. You can do this by utilizing hardware components to be able to link digital combined with the physical by utilizing software packages. This enables individuals to create and access animations and digital information which interacts using the real atmosphere. One of the ways this is accomplished is by a camcorder which utilizes a movement warning or perhaps a image known as marker. Miracle traffic bot is known as augmented reality since it combines digital understanding using the real life which is needed to develop a a lot more smooth and natural consumer experience and boost the use and movement of understanding.
Augmented reality software encompasses a lot of software and options in a number of unique sectors. Miracle traffic bot may be used in knowledge, security, business, gaming, art and much more areas. One of many industries that this specific software is a significant game-changer is cellular marketing.
Web shopping is among the fastest developing industrial sectors and augmented reality software will fully reinvent in what manner people interact and make an online purchase. For example, this unique software has the ability to make it easy for consumers so that you can communicate with a genuine-time picture of an item they are looking at. Which may be done all straight from their property or perhaps on the go. This specific software doubles within mortar and bricks retailers to improve most effective and quickest experience. This can contain interactive demos of products as well as their abilities that cannot be displayed personally in-store.
Other applications include business and knowledge. With the expansion in online learning, this sort of software packages may be employed by teaching experts to possess video lectures together with students and permit students to experience with three-dimensional models, stats and many types of types of additional information. This can enable students to have interaction as well just like when they were personally using the lecturer. This same concept has great options inside the commercial domain also, allowing co-workers to have interaction with each other and understanding from diverse corners around the globe. This can be one very thrilling thing about this type of technology nevertheless the key obstacle is going to be developing the pc software that mixes the pc hardware, data combined with the actual atmosphere The important element for this particular software programs are the interaction of individuals, information along with the real life..
Probably the most fundamental parts and finest problems with the program is unquestionably motion capture additionally to mixing digital camera models combined with the real life with this sort of software. Allowing for customers to interact while using camera by means of gestures and actions easily not to mention will probably be the hallmark of augmented reality software. Consider getting your own personal edition from the Minority Report software packages in your home.


text:
Trading information & trading education tips that are easy to access with a user-friendly interface that works on desktop as well as mobile devices such as iPhones and iPads."
Chicago, IL (PRWEB) October 08, 2013
TradingAdvantage.TV features trading education videos, analysis, market recaps, insights and information, as well as strategy and trading tips; filmed live at the floor of the CME Group, the Chicago Board Options Exchange or at the Trading Advantage headquarters.
"TradingAdvantage.TV fulfills a growing demand for instant access to online trading education, experts' opinions and market analysis," said Larry Levin, Trading Advantage President and CEO. "It provides pertinent trading information & trading education tips that are easy to access with a user-friendly interface that works on desktop as well as mobile devices such as iPhones and iPads."
The network expands the firm's global reach and features a host of Trading Advantage experts, including Levin, Dan O'Brien, Alan Knuckman, Scott Bauer, and Charles Moon, to mention a few.
"It doesn't matter what time zone you're in across the globe, you can immediately get plugged into the information you need with on-demand access to appearances that aired on Fox Business, CNN, Bloomberg News and CNBC" Levin said.
TradingAdvantage.TV allows traders to conveniently track videos by title, topic, date, and add comments.
"Trading Advantage is leading the way in educating and empowering the individual investor, so they can compete in the new technology-driven marketplace," said Levin.
About Trading Advantage
Founded by Larry Levin in 1998, Trading Advantage is a leading trading education firm that offers extensive trading education and courses on futures, options, stocks, day trading, and commodities. The company boasts a professional and experienced instructor team of more than 40 high-caliber industry leaders committed to providing the best trading techniques, strategies, and training. To learn more, please visit http://www.tradingadvantage.com and http://www.tradingadvantage.tv


text:
|It can be a slog, but the view from the top makes it worthwhile
I'm now on my ninth: five published, one on the way to production, one being written and two crime novels currently looking for a good home. The one I'm working on is The Isis Covenant, a follow up to The Doomsday Testament. A couple of weeks ago I was struggling with it, not certain whether I was in the right place or going in the right direction. The advice I always give other writers is that if you have a problem, just keep writing and you'll eventually write your way through it. So that's what I did, but I had a difficult couple of weeks.
The way I explained it to David was that writing a book is like going on one of those long distance walks in the hills where you plan to tackle two or three Munros. You make a start and you're full of beans, then you come to the long climb to the first summit and all you can see is what's directly in front of you. You're groping your way forward one step at at time and progress is slow. Eventually, though, you reach the top and the view ahead is clear, a glorious panorama that stretches for miles ahead. Suddenly everything is worthwhile and you're racing, you know exactly where you're going and how you're going to get there.
I realise now that all I was doing two weeks ago was learning exactly what the book is about, working out the strengths and the weaknesses. Two weeks ago I was probably averaging two thousand words a day - way short of my usual daily target of three thousand - and struggling to do that. This week I wrote an entirely new opening chapter so the book now starts with a bang that increases the pace of the whole first quarter. In five days I wrote twenty thousand words, which is probably my best weekly output ever. No doubt there'll be another Munro to climb before I'm done, but the view from the top makes it all worthwhile.
*The paperback of Hero of Rome came out two weeks ago after quite a big gap, but it looks as if the wait was worthwhile. It sold 1300 copies in the first week in Asda alone and this week it popped up in the Top 10 historical novels at Waterstones. Transworld have ordered the first reprint. A great start and thanks to everyone who went out and bought it!


text:
Agenda for January 25, 2013 TrainingSave this link to your home page by using the Share button in your safari browser
GTKY Activity
Using Camera / Video
- Record Students Speaking!
- Photobooth - fun photos!
- Audio/Podcasting
Basics Settings to Review - Group Time
- AutoLock
- Take Screenshot
- Obtain Image from Internet
- Save a Website to home screen - CCSF ESL Student resources
- PDF to iBooks-100 ESL Games, Article "The Changing Face of ELL..."
- Purchasing of apps
- Find My iPad
- Guided Access Locking a Student in an App
Text to Speech Options
- Highlight to speak using iPad settings
- Subtext (Class Reading Discussion App)
Phonics/Vocabulary Development
- Phonics Genius - primary / elementary
- Quizlet - secondary
- Futaba - a multiplayer app, great for a single iPad center
One Photo Lesson
LunchCreate Digital Story
Story Swap
- Compare and Contrast
- Elaborate
Video Movie MakingResources for Professional Development and Apps
Explore the agenda with more options added


text:
Nongame Wildlife
CDFW Nongame Program
1812 9th Street
Sacramento, CA 95811
(916) 445-0411
Threatened and Endangered Birds
Species Accounts
The list below provides access to species accounts for 33 species and subspecies of birds listed as either threatened or endangered by the State of California or the federal government. This list is provided in alphabetical order, by the general name of the animal (for example: eagle, rail, sparrow, etc).
Each species account contains the latest status report from the Department of Fish and Wildlife's periodic report on the status of these species. Each account also may contain links to additional, available status or life history information.
The list and the accompanying species accounts may not be complete or reflect the current legal status of these birds because of listing activities more recent than the status accounts in the links below. The current legal status for each species is provided online and is updated quarterly.
For more information on the species listed below, use the CDFW's Species Explorer. Please note, the Species Explorer is still being worked on. Not all species might appear in the database.
|Common Name
|Scientific Name
|Albatross, short-tailed
|(Phoebastria albatrus)
|Condor, California
|(Gymnogyps californianus)
|Crane, greater sandhill
|(Grus canadensis tabida)
|Cuckoo, western yellow-billed
|(Coccyzus americanus occidentalis)
|Eagle, bald
|(Haliaeetus leucocephalus)
|Flicker, gilded northern
|(Colaptes auratus chrysoides)
|Flycatcher, willow
|(Empidonax traillii)
|Flycatcher, southwestern willow
|(Empidonax traillii extimus)
|Gnatcatcher, coastal California
|(Polioptila californica californica)
|Goose, Aleutian Canada (RECOVERED)
|(Branta canadensis leucopareia)
|Hawk, Swainson's
|(Buteo swainsoni)
|Murrelet, marbled
|(Brachyramphus marmoratus)
|Owl, great gray
|(Strix nebulosa)
|Owl, northern spotted
|(Strix occidentalis caurina)
|Owl, elf
|(Micrathene whitneyi)
|Plover, mountain
|(Charadrius montanus)
|Plover, western snowy
|(Charadrius alexandrinus nivosus)
|Rail, California black
|(Laterallus jamaicensis coturniculus)
|Rail, California clapper
|(Rallus longirostris obsoletus)
|Rail, light-footed clapper
|(Rallus longirostris levipes)
|Rail, Yuma clapper
|(Rallus longirostris yumanensis)
|Shrike, San Clemente loggerhead
|(Lanius ludovicianus mearnsi)
|Sparrow, Belding's savannah
|(Passerculus sandwichensis beldingi)
|Sparrow, San Clemente sage
|(Amphispiza belli clementeae)
|Swallow, bank
|(Riparia riparia)
|Tern, California least
|(Sterna antillarum browni)
|Towhee, Inyo California
|(Pipilo crissalis eremophilus)
|Vireo, Arizona Bell's
|(Vireo bellii arizonae)
|Vireo, least Bell's
|(Vireo bellii pusillus)
|Woodpecker, Gila
|(Melanerpes uropygialis)


text: India's First Woman Firefighter – Harshini Kanhekar by Jovita Aranha August 2, 2017, 7:14 pm Written by Jovita Aranha A lover of people, cats, food, music, books & films. In that order. Binge-watcher of The Office & several other shows. A storyteller on her journey to document extraordinary stories of ordinary people.


text:
Good Old Fashioned Pancakes
I tweaked it a little by doubling the recipe, adding 1/4 tsp of vanilla and half milk, half buttermilk. Yummmmmmmm!! They are sooo good! Last weekend I added blueberries to the mix, a bit more sugar and BAM! The perfect pancakes. I like using frozen fruits when they aren't in season around here. The kids had seconds and then they had leftovers for a snack. They were so good I barely had to put any syrup on them. I'll be making some this weekend again, so I'll post some pictures then. :)
Now...on to the "Terrible Twos". Can I just say that I'm terrified of Lola turning 3? I really am. She has been just awful. I actually caught her doing something bad. When she didn't think I was looking, I peeked in on her and Lex, saw her grab Lex by the hair and then slap him in the face. I rushed in and guess what she did? She started crying and holding her face saying Lex had hit HER! WHAAAAAAT?!?!?! I could NOT believe what I had just witnessed. I put her in time-out and told her "No!! No hitting! And NO pretending!" I'm fairly certain she understood what I was saying. I mean, HELL, the kid knows enough to lie about the situation, right?! I am in so much trouble. ((sigh)) Just look at her.......
You'd never know she was really a Demon trapped in an adorable, little 2 year old girl. She's going to make a fine teenager. I can just see the meetings now. HAH!


text:
Percy: One year on and I miss my best pal Bart
San Francisco might be the last place Iain Percy wants to base himself but for now it is the place he calls home.
Ainslie determined to bring America's Cup 'home'
Sir Ben Ainslie gets slowly into his stride. It is rare for him to have a chance to draw breath after a relentless 12 months and he is happy to talk.
Iain Percy and Andrew Simpson miss out on gold in dramatic final race
British Star class duo Iain Percy and Andrew Simpson have lost their Olympic title after finishing in silver place in what proved to be a dramatic final day in Dorset.
Iain Percy: London 2012 bid is back on track despite injury scare
Double Olympic sailing champion Iain Percy talks to Metro about going for a third gold in 2012, dealing with creaking bones, and learning from athletes.
Duo round off superb regatta
Iain Percy and Andrew Simpson rounded off another impressive Olympic campaign for Great Britain's sailors when they claimed victory in the Star class to collect the fourth gold and sixth medal overall in China.


text:
Get excited about reading again with fun and interesting tips from our experts, including The M.D., our dietitians, and our fitness expert. In our health library, you will find all of the information you need to achieve your goals of making a healthy lifestyle change. So, start reading and start losing!
Seasonal Diet Tips
Explore our expert knowledge on topics such as healthy eating during the holidays, and how to live into a happier and healthier you in the New Year. Discover tips and tricks on how to avoid weight gain at holiday parties and while on vacation.
Halloween is just around the corner, and for some us, we are still haunted by the weight we gained after last year's Halloween treat trickery... But this year, do not let Halloween candy play a trick on your diet.
Halloween can be a fun time even if being conscientious about your diet, suggesting you do not have to deprive yourself an occasional piece of candy here and there. Have your candy and health, too, with bistroMD's quick guide on managing those Halloween candy temptations!
Choose Wisely
First off, it is alright to enjoy a little Halloween candy! But stressing a little, as too much can sabotage the diet you have worked so hard to clean up. Choose wisely on which pieces of candy you want to enjoy, such as that Reese's Peanut Butter Pumpkin you can only get during Halloween, along with selecting individually-wrapped and small pieces. But if you have difficulty stopping once started, a safer route to go would be choosing hard candy like lollipops that last longer to satisfy your sugar cravings, along with considering these healthier Halloween candy options even kids will appreciate!
Purchase Later in The Month
Following Labor Day, you may be noticing ghosts, witches, and well, that Halloween candy aisle already stocked. But rather than loading up the carts during a bout of excitement, wait to purchase candy later in the month and purchase just a few days in advance. This way, the candy will not be hanging around the house for too long and tempting you to eat it.
Buy Non-Tempting Candy
If you absolutely love milk chocolate, you should probably select a fruity or sour alternative instead. Purchasing non-tempting candy lessens the risk of sneaking a piece here and there and lets the trick-or-treaters take care of its disposal.
Consider a Non-Candy Alternative
Okay, while you may not want to be known as "that neighbor," consider passing out a non-candy alternative. More nutritious options include mini-packages of dried fruit and pretzels. And even if the kids reject them, they may have a parent who will appreciate snacking on a health-conscious Halloween treat! Beyond candy, Halloween-themed pencils, stickers, and glow sticks, are also fun (and non-caloric) suggestions.
Turn Your Cheek On End-of-Season Specials
Following Halloween, stores discount the candy to clear room for the upcoming holiday. And while stocking up on your favorite chocolate may be enticing, turn your cheek to the end-of-season specials. And just think, with Thanksgiving and Christmas coming up, there are bound to be in-season flavors to relish on! (While practicing the concept of moderation, of course...)
Double Up
If you overestimated a headcount or the trick-or-treaters came in short this year, do not hold onto the candy! Start passing out two (or even three) pieces of candy at a time to deplete your resources, ultimately lessening the number of treats around the house. If you are left with any candy, find another way to get rid of it. For example, take it to work or send it to school with your child. They can then give to their teacher, as they can pass out as an occasional reward for a job well done on homework assignments.
Treat Your Body to Exercise
Treats are not always designated to candy... And with your body primed and equipped to be active, let it be! To continue enjoying the Halloween fun, ditch the gym for these Halloween-inspired exercises and joining your kids for a walk around the neighborhood as they trick-or-treat. Treating your body to exercise and nourishing food can not only beat a candy craving, but elevate your mood!
Simply answer a few questions so we can figure out your weight loss goals and provide solutions for a lighter, healthier you. Our weight loss meal plans are designed to help real people achieve real and lasting success.
Get free support to help you lose weight and be healthy with our Weekly Dish on Health.
© 2005-
Privacy Policy - Store Terms
Yes, send me free support to help me lose weight and
be healthy with the Weekly Dish on Health Newsletter.


text:
Thursday Jan 04, 2007
Made by Hand
Today, it often seems that no one, including many an artist, can get a handle of what art is.
To help explain this apparent lack of comprehension, remember that art, at its essence, isn't about fads, trends, taste, or even movements, which are like waves, not the energy creating the waves. Fine art or high art, they are one in the same; this is art from the soul, driven by passion and executed in pure emotion; this art is a source of power and creation that can't be defined by academicians, demystified by the press, or pigeonholed with convenient labels by critics.
Remember too, that anything of quality—art, craft, or design—transcends time and remains forever a thing to be appreciated. Franz Marc's painting Blue Horse I, for example, is as exciting for its spirit, bold colors and purity of form today as it was when he painting it nearly a century ago.
It is really a nice post, its always great reading such posts, this post is good in regards of both knowledge as well as information. Thanks for the post.
montreal asian escorts
It have to be waited by abounding already. The agreement should be accustomed added absorption and trials.
stubby holders
It have to be tremendeous to try this yourself. I am abiding users can get the aforementioned aftereffect with accurate learnings.
stubby holders
I forever missed on wordpress
and here you all give filled in that gap equally advisable. Thank you!
DIY wedding invitations
Can I just say what a relief to find someone like you who actually knows what theyre talking about on the internet. You definitely know how to bring an issue to light and make it important.


text:
- Details
The All Time Low "Emblem Pocket T-Shirt (Heather)" here features a sewn pocket on the upper left chest with the band's initials "ATL" hovering over a cadillac-style design with their heart eye logo in red in the center. Below it is the band's name "All Time Low" in black. The back of the shirt features an enlarged version of the same lettering. The designs are printed on a dark heather grey slim-fit t-shirt with black sleeves.
Please visit our FAQs page to see if your question has already been answered.
There is a link to our contact us form at the bottom if you still need your question answered.


text:
- Research
- Open Access
SNPs in the coding region of the metastasis-inducing gene MACC1 and clinical outcome in colorectal cancer
© Schmid et al.; licensee BioMed Central Ltd. 2012
- Received: 28 November 2011
- Accepted: 13 July 2012
- Published: 29 July 2012
Colorectal cancer is one of the main cancers in the Western world. About 90% of the deaths arise from formation of distant metastasis. The expression of the newly identified gene metastasis associated in colon cancer 1 (MACC1) is a prognostic indicator for colon cancer metastasis. Here, we analyzed for the first time the impact of single nucleotide polymorphisms (SNPs) in the coding region of MACC1 for clinical outcome of colorectal cancer patients. Additionally, we screened met proto-oncogene (Met), the transcriptional target gene of MACC1, for mutations.
We sequenced the coding exons of MACC1 in 154 colorectal tumors (stages I, II and III) and the crucial exons of Met in 60 colorectal tumors (stages I, II and III). We analyzed the association of MACC1 polymorphisms with clinical data, including metachronous metastasis, UICC stages, tumor invasion, lymph node metastasis and patients' survival (n = 154, stages I, II and III). Furthermore, we performed biological assays in order to evaluate the functional impact of MACC1 SNPs on the motility of colorectal cancer cells.
We genotyped three MACC1 SNPs in the coding region. Thirteen % of the tumors had the genotype cg (rs4721888, L31V), 48% a ct genotype (rs975263, S515L) and 84% a gc or cc genotype (rs3735615, R804T). We found no association of these SNPs with clinicopathological parameters or with patients' survival, when analyzing the entire patients' cohort. An increased risk for a shorter metastasis-free survival of patients with a ct genotype (rs975263) was observed in younger colon cancer patients with stage I or II (P = 0.041, n = 18). In cell culture, MACC1 SNPs did not affect MACC1-induced cell motility and proliferation.
In summary, the identification of coding MACC1 SNPs in primary colorectal tumors does not improve the prediction for metastasis formation or for patients' survival compared to MACC1 expression analysis alone. The ct genotype (rs975263) might be associated with a reduced survival for younger colon cancer patients in early stages. However, further studies with larger sample sizes are needed.
- Colorectal cancer
- Metastasis
- Single nucleotide polymorphisms
Colorectal cancer is the third most common form of cancers in the Western world.1 The 5-year-survival rate of colorectal cancer patients with a local tumor is about 90%, whereas only around 10% of the patients survive when distant metastases have formed [1–3].
In an earlier study the new gene metastasis associated in colon cancer 1 (MACC1) was identified by differential display RT-PCR [4]. MACC1 is a prognostic marker for distant metastasis formation and allows the identification of colorectal cancer patients with a high risk for metastatic cancer. Tumors, staged I to III, which developed metachronously metastases, showed a significantly higher MACC1 expression compared to non-metastasizing tumors. The 5-year survival rate for patients with high MACC1 expression in the primary tumors was only 15% compared to 80% for subjects with low MACC1 expression. It was shown that MACC1 is a key regulator of met proto-oncogene (Met) expression [4, 5]. The hepatocyte growth factor (HGF)-Met pathway plays a decisive part in epithelial-mesenchymal transition, cell motility, invasiveness, and metastasis [6, 7]. Overexpression of MACC1 results in Met expression induction and thereby enhances the activation of the MAPK (mitogen-activated protein kinase)-signaling [4].
Various Met mutations are described that lead to high tumorigenicity. Variants of Met were found in many tumor entities, including colon cancer [8–15]. In contrast to Met mutations, MACC1 mutations in tumors have not yet been studied. In databases, single nucleotide polymorphisms (SNPs) are annotated for MACC1. The three MACC1 SNPs identified in this study (rs4721888, rs975263 and rs3735615) are also annotated in databases. However, their occurrence and frequencies in primary tumors and their association with tumor progression and metastasis are unknown.
Thus, the overriding aim of our study was the evaluation of MACC1 SNPs for prognostication of patients' survival. For the first time, we analyzed the mutation status of coding MACC1 exons in colorectal tumors and studied the association of MACC1 SNPs with clinicopathological data, including gender, age, tumor stages, lymph node involvement and, in particular, with the development of distant metastases as well as with overall and metastasis-free survival. Furthermore, we studied the functional effect of the identified MACC1 SNPs on the migratory, proliferative or wound healing potential of colorectal cancer cells by in vitro assays. In addition, we screened the crucial exons of the proto-oncogene Met in colorectal tumors for mutations.
MACC1 SNPs and Met variants in primary colorectal tumors
Furthermore, we studied the coding exons 14 to 19 of one of the transcriptional targets of MACC1, the proto-oncogene Met, in the first 60 colorectal tumors. For this mutation analysis we chose the juxtamembrane and the kinase domain because most of the already described mutations were found in these domains and were described to affect the function of this receptor tyrosine kinase [8, 15]. We found only two tumors with Met mutations: in one tumor the variant R988C (rs45607832) and in a second tumor the variant T1010I (rs56391007) (Figure 1B). Both variants occurred in the juxtamembrane domain (exon 14) of the receptor (Figure 1B). Because of the low minor allele frequency no further tumors were sequenced.
MACC1 SNPs have no impact on MACC1 expression
Moreover, we evaluated the impact of the identified SNPs on the expression of MACC1 in cell culture. We used the colorectal cancer cell lines SW480, DLD1 and HCT116. We introduced SNPs into the wild-type MACC1 sequence by site-directed mutagenesis, cloned the plasmids pcDNA3.1/MACC1/wt, pcDNA3.1/MACC1/L31V, pcDNA3.1/MACC1/S515L, pcDNA3.1/MACC1/R804T, and transfected SW480, DLD1 and HCT116 cells with these constructs. MACC1-transfected cells had an increased MACC1 mRNA (P < 0.01, P < 0.0001, P < 0.01) and protein expression levels compared to parental cells. The identified SNPs, however, did not influence the MACC1 mRNA (P = 0.37, P = 0.54, P = 0.99) or protein expression levels compared to SW480/MACC1/wt, DLD1/MACC1/wt or HCT116/MACC1/wt cells (Figure 2B).
MACC1 SNPs do not affect motility and proliferation of colorectal cancer cells
Moreover, we studied the proliferative potential of the transfected cells. Cells with one of the constructs pcDNA3.1/MACC1/L31V, pcDNA3.1/MACC1/S515L or pcDNA3.1/MACC1/R804T showed the same ability for proliferation as cells with the wild-type construct. Thus, none of the SNPs affects the proliferative potential of these colorectal cancer cells (Figure 3B).
Furthermore, we analyzed the wound healing ability of these cells. MACC1 promotes the ability of cells to close a wound [4]. We confirmed that SW480/MACC1/wt, DLD1/MACC1/wt and HCT116/MACC1/wt cells do close the wound area faster than parental cells, but transfectants harboring the MACC1 SNPs L31V, S515L, and R804T did not demonstrate an altered potential for directed migration (Figure 3C).
MACC1 SNPs and association with metastasis or clinicopathological parameters
Association of MACC1 SNPs with clinicopathological factors
rs4721888 (L31V)
Leu/Leu (ctc/ctc) (%)
Leu/Val (ctc/gtc) (%)
All tumors, n = 154
134 (87%)
20 (13%)
Metachronous metastasis,n = 29
27 (93%)
2 (7%)
0.10 - 2.02
Gender male, n = 79
68 (86%)
11 (14%)
0.46 - 3.05
Age > 66.32 years (median),n = 77
67 (87%)
10 (13%)
0.39 – 2.56
pT3 and pT4, n = 118
102 (86%)
16 (14%)
0.39 - 4.03
N1 to N3, n = 53
43 (81%)
10 (19%)
0.82 - 5.47
UICC stage I, n = 26
24 (92%)
2 (8%)
0.11 - 2.34
UICC stage II, n = 74
66 (89%)
8 (11%)
0.26 - 1.79
UICC stage III, n = 54
44 (81%)
10 (19%)
0.79 - 5.28
rs975263 (S515L)
Ser/Ser (tcg/tcg) (%)
Ser/Leu (tcg/ttg) (%)
All tumors, n = 154
80 (52%)
74 (48%)
Metachronous metastasis,n = 29
12 (41%)
17 (59%)
0.75 – 3.83
Gender male, n = 79
40 (51%)
39 (49%)
0.59 - 2.10
Age > 66.32 years (median),n = 77
40 (52%)
37 (48%)
0.53 – 1.88
pT3 and pT4, n = 118
63 (53%)
55 (47%)
0.37 - 1.65
N1 to N3, n = 53
26 (49%)
27 (51%)
0.61 - 2.32
UICC stage I, n = 26
14 (54%)
12 (46%)
0.39 - 2.13
UICC stage II, n = 74
39 (53%)
35 (47%)
0.50 - 1.78
UICC stage III, n = 54
27 (50%)
27 (50%)
0.58 - 2.19
rs3735615 (R804T)
without R804T compared to with R804T
Arg/Arg (aga/aga) (%)
Arg/Thr (aga/aca) (%)
Thr/Thr (aca/aca) (%)
All tumors, n = 154
25 (16%)
74 (48%)
55 (36%)
Metachronous metastasis,n = 29
5 (17%)
15 (52%)
9 (31%)
0.33 - 2.80
Gender male, n = 79
11 (14%)
37 (47%)
31 (39%)
0.60 - 3.36
Age > 66.32 years (median),n = 77
12 (16%)
39 (51%)
26 (34%)
0.47 - 2.59
pT3 and pT4, n = 118
21 (18%)
53 (45%)
44 (37%)
0.18 - 1.81
N1 to N3, n = 53
9 (17%)
27 (51%)
17 (32%)
0.38 - 2.25
UICC stage I, n = 26
4 (15%)
14 (54%)
8 (31%)
0.34 - 3.46
UICC stage II, n = 74
12 (16%)
32 (43%)
30 (41%)
0.43 - 2.36
UICC stage III, n = 54
9 (17%)
28 (52%)
17 (32%)
0.39 - 2.33
MACC1 SNPs and association with overall survival (OS) and metastasis-free survival (MFS) in all colorectal cancer patients
Combinations of SNPs, e.g. patients with the variant allele 31 LV and 515 SL, with 31 LV and 804 RT/TT, or with 515 SL and 804 RT/TT, compared to patients without any SNP or with only one SNP did not improve survival prognosis.
Younger colon cancer patients of early stages with a genotype 515 SL have a reduced MFS
The metastasis-inducing gene MACC1 is a newly identified gene and its expression is a prognostic indicator for colorectal cancer metastasis [4]. In this study, we identified the SNPs rs4721888, rs975263, rs3735615 in the coding region of MACC1 in primary colorectal tumors. We analyzed the association of the MACC1 SNPs with clinical parameters such as age, gender as well as with metachronous metastasis, UICC-stages, tumor and lymph node infiltration. However, none of the identified variants 31 LV, 515 SL, and 804 RT or 804 TT was associated with one of these parameters. Furthermore, the identified SNPs were neither associated with OS nor with MFS in the entire cohort of colorectal cancer patients analyzed. However, colon cancer patients classified to UICC-stage I or II and younger than 60 years old with a 515 SL variant had a decreased MFS time. Thus, the T-allele of this SNP might be associated with the survival of younger patients with an early staged colon tumor. Nevertheless, our analyzed subgroup is very small and validation by comprehensive cohorts is necessary.
We identified three different MACC1 SNPs and two Met variants in the colorectal tumors that are already annotated in the NCBI SNP database. The occurrence and frequencies of MACC1 SNPs in primary tumors of colorectal cancer patients were unknown but their frequencies in different non-cancer populations can be found in SNP databases. Only two tumors showed variants of the proto-oncogene Met. Other studies confirmed that Met mutations are rare events [11, 16]. Tyner and colleagues reported that the variant R988C occurs only in 1% (95% CI = 0-5%) and T1010I in 0% (95% CI = 0-3%) of 109 colorectal tumors [11]. In our analyzed tumors both Met variants appear in only 1.6% of all tumors. The effects of the Met variants are controversially discussed. Schmidt et al. reported that T1010I does not result in a constitutive phosphorylation of Met in NIH3T3 cells [15]. Lee et al. confirmed the conclusion, but showed that mice tumors with this Met variant grow slightly faster [12]. Finally, Tyner et al. described no difference in the transforming capacity or in the phosphorylation status of the variants compared to the wild-type receptor [11]. Due to the few Met variants in our tumor panel a correlation to clinical data was not possible.
It was shown that various SNPs of cancer-related genes are associated with a higher risk or a faster progression of cancers [17–19]. Examples of activating mutations are the missense mutations in codon 12 or 13 of the KRAS genes that lead to conformational changes in the KRAS protein [20]. In SNP databases 55 missense SNPs in the coding region of MACC1 are annotated. All three identified MACC1 SNPs in this study are missense alterations. SNP L31V leads to an amino acid exchange from leucine to valine. An influence on the protein structure is disputable because both amino acids belong to the group of nonpolar amino acids. SNP S515L results in a substitution of serine for leucine. Here, a polar charged amino acid is replaced by a nonpolar one and this may result in a loss of a possible phosphorylation site. SNP R804T exchanges the amino acid sequence from arginine to threonine that potentially can act as phosphorylation site. Thus, an effect on the protein function could be possible. We used SIFT and Polyphen software tools for the estimation of functional impact [21, 22]. Both programs predict that the 31 VL and 515 SL variants are probably benign, whereas 804 RT could be damaging. R804T lies in a conserved domain and might have therefore a decisive role. SNPs L31V and S515L are not in predicted domains of the protein structure, whereas R804T can be found in a putative death domain. So far, the MACC1 protein domain architecture was exclusively studied by bioinformatical approaches [23]. Further efforts are required to elucidate the MACC1 protein structure in order to evaluate the importance of the identified SNPs. We analyzed the possible effect of these MACC1 SNPs on the protein function by biological in vitro assays. First, we showed that the SNPs do not change the expression level of MACC1. We then performed migration and proliferation assays but did not observe an influence of the SNPs on the behavior of the colorectal cancer cells. The MACC1 variant 804 RT or 804 TT which was predicted as possibly damaging did not have a functional impact on cell migration and proliferation studies.
This study has some limitations. First, as already mentioned, the MACC1 SNP analysis should also be carried out with a larger cohort of patients. Further efforts should be made in order to evaluate if the MACC1 SNPs are germline or somatic polymorphisms and if they occur in other tumor entities. Moreover, other regions of the MACC1 gene that are more likely related to its expression, such as the promoter region or the 3'UTR where possible miRNAs could bind, should be explored.
Taken together, the analysis of the coding MACC1 variants 31 LV, 515 SL, 804 RT or 804 TT in primary colorectal tumors does not improve the prediction for metastasis formation or for patients' survival compared to MACC1 expression analysis alone. Thus, a general screening for these MACC1 SNPs in colorectal cancer patients is not recommended. This first study suggests that the MACC1 SNP S515L should be validated in a further study in order to prove a possible prognostic value for this SNP.
Characteristics of colorectal cancer patients
n (%)
154 (100%)
79 (51%)
75 (49%)
Age at diagnosis (years)
Median ± SD: 66.32 ±10.44
< 60
40 (26%)
57 (37%)
> 70
57 (37%)
105 (68%)
49 (32%)
Metachronous metastasis
29 (19%)
125 (81%)
UICC stage
26 (17%)
74 (48%)
54 (35%)
pT status
pT1 + 2
36 (23%)
pT3 + 4
118 (77%)
pN status
101 (66%)
53 (34%)
Microdissection and isolation of DNA and RNA
Serial cryosections of each tissue were evaluated by a pathologist. The tumor cell populations were microdissected and the DNA was isolated with the QIAamp Minikit (Qiagen) according to the manufacturer's protocol. Total RNA was extracted by TRIzol/Chloroform (Invitrogen).
Polymerase chain reaction (PCR) and sequencing
Primers and probes used for PCR and qRT-PCR
Sequence 5'-3'
MACC1, exon 4
Forward primer
Reverse primer
MACC1, exon 5.1
Forward primer
Reverse primer
MACC1, exon 5.2
Forward primer
Reverse primer
MACC1, exon 5.3
Forward primer
Reverse primer
MACC1, exon 5.4
Forward primer
Reverse primer
MACC1, exon 5.5
Forward primer
Reverse primer
MACC1, exon 6
Forward primer
Reverse primer
MACC1, exon 7
Forward primer
Reverse primer
Met, exon 14
Forward primer
Reverse primer
Met, exon 15
Forward primer
Reverse primer
Met, exon 16
Forward primer
Reverse primer
Met, exon 17
Forward primer
Reverse primer
Met, exon 18
Forward primer
Reverse primer
Met, exon 19
Forward primer
Reverse primer
Forward primer
Reverse primer
Forward primer
Reverse primer
Forward primer
Reverse primer
MACC1, qRT-PCR
Forward primer
Reverse primer
Quantitative reverse transcription–polymerase chain reaction (qRT-PCR)
QRT-PCR was carried out using the LightCycler480 (Roche) as described previously [4]. QRT-PCR was performed with the FastStart DNA Master HybProbe Kit (Roche) according to the manufacturer's instructions. The primers and probes are listed in Table 3. In addition, cDNA quantification of the housekeeping gene glucose-6-phosphate dehydrogenase (Roche) was performed. For each qRT-PCR reaction a mean of duplicates was calculated and normalized to the respective mean of the housekeeping gene cDNA.
Site-directed mutagenesis
The MACC1 SNPs L31V, S515L and R804T were introduced into the previously described plasmid pcDNA3.1D/MACC1-V5-His by site-directed mutagenesis (QuikChange XL Site-Directed Mutagenesis Kit, Stratagene) [4]. The primers are summarized in Table 3. The constructs pcDNA3.1/MACC1/wt, pcDNA3.1/MACC1/L31V, pcDNA3.1/MACC1/S515L and pcDNA3.1/MACC1/R804T were used for transfection of colorectal cancer cells.
Transfection of SW480, DLD1 and HCT116 cells
Human colorectal cancer cells SW480 were grown in RPMI-1640 (PAA) containing 10% fetal calf serum (Biochrom), DLD1 and HCT116 cells were grown in DMEM (PAA) containing 10% fetal calf serum. The cells were cultured in a humidified 5% CO2 atmosphere. Cells were analyzed by PCR and found to be free of mycoplasms. Authentification of SW480, DLD1 and HCT116 cells was performed by short tandem repeat (STR) genotyping (Leibniz-Institut DSMZ, Braunschweig, Germany) STR genotype was consistent with published genotype. Cells were transfected with the plasmids pcDNA3.1/MACC1/wt, pcDNA3.1/MACC1/L31V, pcDNA3.1/MACC1/S515L, pcDNA3.1/MACC1/R804T by using Fugene HD (Roche) according to the manual.
Protein extraction and Western blotting
For protein isolation cells were harvested and incubated on ice with RIPA buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% Nonidet P-40, protease inhibitor tablets; Roche). Protein concentration was quantified with Coomassie Plus (Bradford) Protein Assay (Pierce). Fifty μg of the lysates were loaded on a NuPAGE Bis-Tris Gel (Invitrogen), separated, and transferred onto a nitrocellulose membrane. Membranes were incubated in blocking solution and afterwards incubated with rabbit anti-human MACC1 (1:1.000, Sigma) or mouse anti-human β-tubulin (1:1.000, BD Pharmingen) antibody, and subsequently with HRP-conjugated anti-rabbit IgG (1:10.000, Promega) or goat anti-mouse IgM (1:10.000, Sigma) antibody, respectively. At least 3 independent experiments were performed.
Migration, proliferation and wound healing assays
Cell migration assay of the cell lines SW480, DLD1, HCT116 and their cell clones was performed in transwell Boyden chambers (Invitrogen). Cells that passed the pores and migrated through the lower chamber were counted after 24 h. For the proliferation assays cells were treated daily with 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2 H-tetrazolium bromide (MTT; Sigma), crystallized MTT was resolved with dimethylsulfoxid (Applichem), and the optical density was measured at 560 nm. For the wound healing assays cells were seeded into wells, scratched, and the wound healing area was documented until day 4. Assays were repeated 3 times for each clone, respectively.
Statistical analysis was performed with GraphPad Prism version 5. The comparison of two groups was done by two-sided Student's t-test. Several groups were compared by one-way analysis of variance (ANOVA) and Bonferroni post hoc multiple comparison. The significance of clinical parameters was evaluated by Mann–Whitney tests and Chi-Square-tests with one degree of freedom. The odds ratio (OR) or hazard ratio (HR) with corresponding 95% confidence intervals (CI) were calculated. The Kaplan–Meier method was used to estimate cumulative survival rates, and differences in survival rates were assessed using the Log-Rank test. The OEGE software was used for testing the Hardy-Weinberg equilibrium (HWE) using the Chi-Square-test with one degree of freedom [24]. For the determination of the linkage disequilibrium the correlation coefficient r2 and D' was calculated by using OEGE software [25]. Statistical significance was considered for P < 0.05.
We thank W. Haensch for pathohistological evaluation of the tumors and W. Kemmner and his group for the contribution of tumor DNA. We are grateful to C. Lemos for critically reading the manuscript and M. Osterland and M. Niederstrasser for support concerning statistical analyses.
Authors' Affiliations
- Jemal A, Siegel R, Ward E, Hao Y, Xu J, Thun MJ: Cancer statistics. CA Cancer J Clin. 2009, 59: 225-249. 10.3322/caac.20006View ArticlePubMedGoogle Scholar
- Christofori G: New signals from the invasive front. Nature. 2006, 441: 444-450. 10.1038/nature04872View ArticlePubMedGoogle Scholar
- Stein U, Schlag PM: Clinical, biological and molecular aspects of metastasis in colorectal cancer. Recent Results Cancer Res. 2007, 176: 61-80. 10.1007/978-3-540-46091-6_7View ArticlePubMedGoogle Scholar
- Stein U, Walther W, Arlt F, Schwabe H, Smith J, Fichtner I, Birchmeier W, Schlag PM: MACC1, a newly identified key regulator of HGF-MET signalling, predicts colorectal cancer metastasis. Nat Med. 2009, 15: 59-67. 10.1038/nm.1889View ArticlePubMedGoogle Scholar
- Stein U, Smith J, Walther W, Arlt F: MACC1 controls Met: what a difference an Sp1 site makes. Cell Cycle. 2009, 8: 2467-2469. 10.4161/cc.8.15.9018View ArticlePubMedGoogle Scholar
- Birchmeier C, Birchmeier W, Gherardi E, Vande Woude GF: Met, metastasis, motility and more. Nat Rev Mol Cell Biol. 2003, 4: 915-925. 10.1038/nrm1261View ArticlePubMedGoogle Scholar
- Jiang W, Hiscox S, Matsumoto K, Nakamura T: Hepatocyte growth factor/scatter factor, its molecular, cellular and clinical implications in cancer. Crit Rev Oncol Hematol. 1999, 29: 209-248. 10.1016/S1040-8428(98)00019-5View ArticlePubMedGoogle Scholar
- Schmidt L, Duh FM, Chen F, Kishida T, Glenn G, Choyke P, Scherer SW, Zhuang Z, Lubensky I, Dean M, Allikmets R, Chidambaram A, Bergerheim UR, Feltis JT, Casadevall C, Zamarron A, Bernues M, Richard S, Lips CJ, Walther MM, Tsui LC, Geil L, Orcutt ML, Stackhouse T, Lipan J, Slife L, Brauch H, Decker J, Niehans G, Hughson MD, Moch H, Storkel S, Lerman MI, Linehan WM, Zbar B: Germline and somatic mutations in the tyrosine kinase domain of the MET proto-oncogene in papillary renal carcinomas. Nat Genet. 1997, 16: 68-73. 10.1038/ng0597-68View ArticlePubMedGoogle Scholar
- Ma PC, Maulik G, Christensen J, Salgia R: c-Met: structure, functions and potential for therapeutic inhibition. Cancer Metastasis Rev. 2003, 22: 309-325. 10.1023/A:1023768811842View ArticlePubMedGoogle Scholar
- Maulik G, Shrikhande A, Kijima T, Ma PC, Morrison PT, Salgia R: Role of the hepatocyte growth factor receptor, c-Met, in oncogenesis and potential for therapeutic inhibition. Cytokine Growth Factor Rev. 2002, 13: 41-59. 10.1016/S1359-6101(01)00029-6View ArticlePubMedGoogle Scholar
- Tyner JW, Fletcher LB, Wang EQ, Yang WF, Rutenberg-Schoenberg ML, Beadling C, Mori M, Heinrich MC, Deininger MW, Druker BJ, Loriaux MM: MET receptor sequence variants R970C and T992I lack transforming capacity. Cancer Res. 2010, 70: 6233-6237. 10.1158/0008-5472.CAN-10-0429PubMed CentralView ArticlePubMedGoogle Scholar
- Lee JH, Han SU, Cho H, Jennings B, Gerrard B, Dean M, Schmidt L, Zbar B, Vande Woude GF: A novel germ line juxtamembrane Met mutation in human gastric cancer. Oncogene. 2000, 19: 4947-4953. 10.1038/sj.onc.1203874View ArticlePubMedGoogle Scholar
- Schmidt L, Junker K, Weirich G, Glenn G, Choyke P, Lubensky I, Zhuang Z, Jeffers M, Vande Woude G, Neumann H, Walther M, Linehan WM, Zbar B: Two North American families with hereditary papillary renal carcinoma and identical novel mutations in the MET proto-oncogene. Cancer Res. 1998, 58: 1719-1722.PubMedGoogle Scholar
- Park WS, Dong SM, Kim SY, Na EY, Shin MS, Pi JH, Kim BJ, Bae JH, Hong YK, Lee KS, Lee SH, Yoo NJ, Jang JJ, Pack S, Zhuang Z, Schmidt L, Zbar B, Lee JY: Somatic mutations in the kinase domain of the Met/hepatocyte growth factor receptor gene in childhood hepatocellular carcinomas. Cancer Res. 1999, 59: 307-310.PubMedGoogle Scholar
- Schmidt L, Junker K, Nakaigawa N, Kinjerski T, Weirich G, Miller M, Lubensky I, Neumann HP, Brauch H, Decker J, Vocke C, Brown JA, Jenkins R, Richard S, Bergerheim U, Gerrard B, Dean M, Linehan WM, Zbar B: Novel mutations of the MET proto-oncogene in papillary renal carcinomas. Oncogene. 1999, 18: 2343-2350. 10.1038/sj.onc.1202547View ArticlePubMedGoogle Scholar
- Park WS, Oh RR, Kim YS, Park JY, Shin MS, Lee HK, Lee SH, Yoo NJ, Lee JY: Absence of mutations in the kinase domain of the Met gene and frequent expression of Met and HGF/SF protein in primary gastric carcinomas. APMIS. 2000, 108: 195-200. 10.1034/j.1600-0463.2000.d01-44.xView ArticlePubMedGoogle Scholar
- Kemp Z, Thirlwell C, Sieber O, Silver A, Tomlinson I: An update on the genetics of colorectal cancer. Hum Mol Genet. 2004, 13: R177-R185. 10.1093/hmg/ddh247View ArticlePubMedGoogle Scholar
- Lo YL, Yu JC, Chen ST, Yang HC, Fann CS, Mau YC, Shen CY: Breast cancer risk associated with genotypic polymorphism of the mitosis-regulating gene Aurora-A/STK15/BTAK. Int J Cancer. 2005, 115: 276-283. 10.1002/ijc.20855View ArticlePubMedGoogle Scholar
- van Wyk R, Slezak P, Hayes VM, Buys CH, Kotze MJ, de Jong G, Rubio C, Dolk A, Jaramillo E, Koizumi K, Grobbelaar JJ: Somatic mutations of the APC, KRAS, and TP53 genes in nonpolypoid colorectal adenomas. Genes Chromosomes Cancer. 2000, 27: 202-208. 10.1002/(SICI)1098-2264(200002)27:2<202::AID-GCC13>3.0.CO;2-3View ArticlePubMedGoogle Scholar
- Scheffzek K, Ahmadian MR, Kabsch W, Wiesmüller L, Lautwein A, Schmitz F, Wittinghofer A: The Ras-RasGAP complex: structural basis for GTPase activation and its loss in oncogenic Ras mutants. Science. 1997, 277: 333-338. 10.1126/science.277.5324.333View ArticlePubMedGoogle Scholar
- Kumar P, Henikoff S, Ng PC: Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm. Nat Protoc. 2009, 4: 1073-1081. 10.1038/nprot.2009.86View ArticlePubMedGoogle Scholar
- Adzhubei IA, Schmidt S, Peshkin L, Ramensky VE, Gerasimova A, Bork P, Kondrashov AS, Sunyaev SR: A method and server for predicting damaging missense mutations. Nat Methods. 2010, 7: 248-249. 10.1038/nmeth0410-248PubMed CentralView ArticlePubMedGoogle Scholar
- Kokoszyńska K, Kryński J, Rychlewski L, Wyrwicz LS: Unexpected domain composition of MACC1 links MET signaling and apoptosis. Acta Biochim Pol. 2009, 56: 317-323.PubMedGoogle Scholar
- Rodriguez S, Gaunt TR, Day IN: Hardy-Weinberg equilibrium testing of biological ascertainment for Mendelian randomization studies. Am J Epidemiol. 2009, 169: 505-514.PubMed CentralView ArticlePubMedGoogle Scholar
- Gaunt TR, Rodríguez S, Day IN: Cubic exact solutions for the estimation of pairwise haplotype frequencies: implications for linkage disequilibrium analyses and a web tool 'CubeX'. BMC Bioinformatics. 2007, 8: 428- 10.1186/1471-2105-8-428PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


text:
The house is a shape everyone has some form of relationship with. Whether it symbolizes comfort, global financial crises in housing market, cookie cutter mediocrity or family, the house as a mundane symbol or object has been elevated to captivating experimental art and high art on several occasions. This weekend we share with you a selection of significant works that adapt houses into art objects.
Urs Fischer's Untitled (Bread House), constructed of bread, bread crumbs, wood, polyurethane foam, silicone, acrylic paint, screws, tape and rugs leaves every ingredient exposed. Stepping inside this large sculptural work recently at MOCA had the effect of walking inside a decaying fairytale, as the work is naturally allowed to crumble and decompose in exhibition. Stepping over piles of crusts of cinnamon raisin bread amidst dirty rugs and peering up at the bubbled polyeurythane foam that seeps between boards and rows of old bread, the viewer may feel any combination of wonder, amusement and fear- much like Grimms Brothers Fairytales.
An Te Liu's Title Deed evolved from the Leona Drive Project in Toronto where a number of vacant tract houses were offered to artists to be reinvented as artistic installations. As this project took place in 2009 in the height of the housing market crash, the artist observed that the simple shape of the existing house represented the 20th century iconic Monopoly board game house pieces. The simple, yet flawless execution of Title Deed situated within a functioning suburban neighborhood carries comical yet heavy implications.
In other instances artists have used the space within the house as the content for their works. In a beautiful demonstration of negative space becoming the content of an artwork, Rachel Whiteread, who has mad a practice of casting large structures in concrete for a number of years, cast the entire interior of a Victorian era home. This piece, entitled House, is a mystifying work whereby the emptiness that was once in the house determined all the substance that physically remains. It stands as a haunting and profoundly poetic beacon on an empty street.
Olga Koumoundouros recently took over the foreclosed home next to hers, and used it as a platform to interject many fascinating artistic conceptual tangents. The project A Notorious Possession began with the artist painting the exterior of the home entirely in gold, evolved into the interior of the home's tranformation into a site specific work of art, which incorporated furniture left in the space and a radiant rainbow painted band that bisects through the home, appearing to touch all the objects and upend them. A Notorious Possession has evolved beyond its initial space and into art spaces, where elements have been re-staged.
Dan Havel and Dean Ruck created Tunnel House a few years ago by peeling away the "skin" of two neighboring houses and using it to make a spiraling vortev that connected one exterior wall to another. Unfortunately this piece is no longer standing, which is a shame, as the interactive quality of this work delivers the experience of an adult playground.
The late Mike Kelley's Mobile Homestead, completed at the time of the artist's death, is an exact replica of his childhood home in Detroit, with sponsorship by MOCAD. The work is at once a public sculpture and an intimate architectural work. Iain Baxter&'s (writer's note: the & is not a type, it is an official part of the conceptual artist's name) work Bagged Place, originally created in 1966 was recreated recently in London. After Rebecca Levy, who had lived in the same home (above the art gallery Raven Row) from 1918-2009, passed away, the gallery invited Iain to transform her home into an artwork. In Rebecca's Bagged Place every single personal item in her home is bagged or wrapped in plastic- every surface and corner covered- in a statement of the commodification of possessions and experiences.


text:
Randy Wachtler, one of Full Sail's First Grads, Talks About Life at Full Sail in 1979
When Full Sail was founded back in 1979, the school only offered one program: a six-week recording workshop based out of a studio in Altamonte Springs, Florida. Randy Wachtler, a music composer with more than 30 years experience in the industry, was one of the first people to attend the program, and last week, he visited Full Sail's campus for the first time since graduating. We sat down with him for a few minutes to hear about his memories from attending Full Sail more than 30 years ago.
Q: What originally drew you to Full Sail's program in 1979?
A: I think it was the only thing that I could find at the time that actually allowed us to go into the studio and showed us how to record. Prior to that, I don't think there was anything available that I knew of that [allowed for that].
Q: What memories do you have of the six-week program at Full Sail?
A: I thought it was the coolest thing to come into this recording studio and get to be involved. I played drums on our little class piece called "Take the A-Train," which we recorded. It was really my first time being in a real recording studio. The teachers [including Full Sail President Garry Jones] were very professional. For a guy like me who had just played in bands, this was really great, getting to see how records were made and what the techniques were, like where you placed the microphones. I just remember thinking that was really cool.
Q: Did you originally want to do music production when you went to Full Sail?
A: I think I was one of those weird guys that was always thinking about other music. All of my friends were focused on the artists and the bands and the record companies, and I was kind of more like, "Okay everybody wants to do that." So everybody was zigging and I wanted to zag. I wanted to find out about all the other music you don't pay attention to: Where does that come from? So I became very interested in learning as much as I could about that.
Q: Is this your first time to campus?
A: Yes, and I'm absolutely blown away. It's unbelievable to me that it went from that little 25-person group to a 192-acre campus with 37,000 graduates. Just to see the graduates on the walls here, the Hall of Fame, and where Full Sail has placed people is so, so impressive. I've always seen Full Sail ads, so I knew that it had to be growing, but I had no idea to this level.
2 thoughts on "Randy Wachtler, one of Full Sail's First Grads, Talks About Life at Full Sail in 1979"
The original location of the workshop and studio was actually Orlando just off Lee Rd in a Studio called Bee Jay recording studio. It was a few years later it relocated to the Douglas Avenue location next to what was then Park Studios.
John Yandell's comment below is correct. John Phelps, founder of Full Sail Recording Workshop, approached Bee Jay Recording Studios and asked the staff to help create the original curriculum for Full Sail and to provide the major share of the Bee Jay staff to be instructors. Bee Jay Studios also provided the studio / class rooms. FS later moved to the Douglas Avenue Address in Altamonte Springs. I know because I was their and made the deal. Eric T. Schabacker, Former Founder,Owner and President / Bee Jay Recording Studios, Inc.
Comments are closed.


text:
Scientists claim to have developed a synthetic molecule that causes cancer cells to self-destruct, potentially leading to effective new treatments for the disease.
Both cancerous and normal body cells contain a protein called procaspase-3 which, when activated, prompts the cell to release toxins, killing itself.
However, in cancerous cells, the process of activation does not occur, allowing tumours to grow.
But now a team of researchers from the University of Illinois have developed a protein that is directly able to activate procaspase-3, initiating the suicide mechanism in the cancer cells.
"We have identified a small, synthetic compound that directly activates procaspase-3 and induces apoptosis (cell death)," explained Professor Paul Hergenrother, a member of the research team.
"By bypassing the broken pathway, we can use the cells' own machinery to destroy themselves."
The discovery opens the door to the possibility of new cancer fighting treatments that are able kill off cancer cells without the need for more harmful radiation or chemotherapy drugs.
"This is the first in what could be a host of organic compounds with the ability to directly activate executioner enzymes," added Professor Hergenrother.


text:
Minuteman Press Cape Town
Monday to Friday 8am-5pm, Saturday 9am-1pm
Closed on Public Holidays and Saturdays that fall on long weekends
Monday to Friday 8am-5pm, Saturday 9am-1pm
Closed on Public Holidays and Saturdays that fall on long weekends
Monday to Friday 8am-5pm, Saturday 9am-1pm
Closed on Public Holidays and Saturdays that fall on long weekends
We pride ourselves on meeting agreed deadlines and providing quality printed product and excellent service.
Below is a sample of the printing and promotional products and services we provide, not to mention top of range print finishing solutions such as hot stamp foiling, embossing, laminating and booklet making. To learn more about what we can do for you, contact one of our representatives today.
Business StationeryBusiness Cards, Letterheads & Envelopes, Presentation Folders, Carbonless Forms (e.g. Invoice Books), Writing Pads
Marketing MaterialFlyers & Brochures, Booklets, Newsletters, Calendars, Postcards, Die cut wobblers
Operational StationeryPresentations & Proposals, Training Course Materials, Conferences & Events , Certificates, Stickers & Labels, Menus, Invitations
Digital PrintingDigital colour and B&W Bulk Copying, Variable Data Printing, Scanning,
Large Format & DisplayPosters, Pull-up Banners, Display Units (snapper , hanging & wall-mounted frames as well as free-standing units), Outdoor Banners, Vinyl Stickers / Decals (cut-out). Plan plotting
Conference PrintingComplete solution offered including name badges, seating plans, manuals, programmes, roll-up banners, convenient delivery to your hotel or convention centre (CTICC). Recognised Mining Indaba printers for delegates at the annual event in Cape Town. Numerous other refererences local and international.
T-Shirts and HoodiesT-Shirts printing, Hoodies printing
Corporate Clothing & GiftingLarge selection of quality apparel and gift ideas, with your corporate branding.
Specialised ItemsEvent Tickets, Rubber Stamps, Menus, Magnets, Wedding Invitations, Special Event Invitations
Digital Printing & CopyingDigital full colour printing on small and large format with top of the range production equipment. B&W bulk copying, variable data printing and scanning services. Paperight book printing.
Finishing & BinderyStandard Print Finishing functions such as binding, laminating, cutting, scoring & folding, drilling, collating, die cutting, embossing, numbering & perforating are done efficiently and neatly. Finishing skills for bespoke printing including foiling, embossing and die cutting are our speciality
Graphic DesignGraphic design, layout, and logo design
Litho PrintingWas the colour of your last full colour litho print job anicolor? If not you need to ask yourself why not.....but then what is Anicolor? Anicolor is a remarkable technology where litho printing meets digital.
Walk-in ServiceOver-the-counter quick, high-quality, cost-efficiant printing and copying services


text:
Denver's Finest Heirloom Portraits
We believe that traditional portraits are the way of appreciating emotions, love, memories. It is the visual culmination of our efforts in life. The love of our life, family, ambition, and accomplishments.
Satterly Portraiture operates solely to provide our clients with time honored art pieces. Here are the reasons to book your portrait session with Satterly Portraiture.
"I didn't realize the magnitude my Satterly Portrait would have on my family. Lisa captured an immortal pose of my daughter at her first communion that invoked such emotion with my wife. Someday my daughter will understand the depths of our love in that moment and will be able to share that with her own family someday. Now we can share that love with her always. Even when we are not with her anymore. This is the portrait that outlives the moment. Thank you Lisa!"
-David B.
"My grandparents had portraits done like this and it came out very nice, the attention to detail is amazing. If you want something to last forever - to leave a legacy - I'd highly recommend having a Satterly Portrait done for yourself or for your family. If you want something really, really special, I recommend Lisa and her team at Satterly Portraits"
-Damian W.


text:
Vineyard & Winery | Ditchling, East Sussex, England.
Court Garden
Classic Collection
Our Classic Collection features six bottles of our multi-award-winning English Sparkling Wines; one of each of 2013 Court Garden Rosé, 2013 Blanc de Blancs and 2013 Blanc de Noirs, and three bottles of 2014 Classic Cuvée, delivered free in the UK.
- Case price:
- £152.00
- UK Delivery:
- Box saving:
- £10
view basket
|Pay securely with:


text:
Tuesday, 24 February 2015
8 comments:
- orvokki said...
Obviously, the spring has arrived.
in the old days encyclopaedias were heavy, and they had a number of parts. I think google time is better :)
We call this "pajunkissa" (wicker cat)
- 24 February 2015 at 20:39
- VP said...
Pussy willows? That's a name!
- 24 February 2015 at 20:53
- William Kendall said...
I wonder if it's a North American term for them- that's what we've always called them.
- 24 February 2015 at 22:15
- Michelle said...
I have to say that I think Wicker Cat is a much better name. lol
- 25 February 2015 at 01:48
- Denton Harryman said...
Kay, from Sequim WA Daily Photo, recently shared a photo of pussy willows .. in her description she borrowed a line from a Jimi Hendrix,"scuse me while I kiss the sky"
- 25 February 2015 at 02:46
- Karl Demetz said...
Nice, the first signs of spring !
- 25 February 2015 at 11:38
- Aimeecakes said...
I have no clue how we survived before google!! Spring is my favourite time of the year!
- 25 February 2015 at 15:20
- EG CameraGirl said...
I am looking forward to seeing pussy willows! Looks like you will see spring before I do.
It's hard to believe there was life before Google.
- 26 February 2015 at 16:21


text:
Every now and again, one of our distributors will offer a "One Day Sale" on a wine. As you can ascertain, we get a discounted price on a particular wine, which is usually something high-end. That leaves me with a decision. Do I leave the wine at the same price and make some extra money or do I pass along the savings to our customers? PASS ALONG THE SAVINGS TO OUR CUSTOMERS!!!!!!
The wine that was featured in the "One Day Sale" was the 2004 Bertani Amarone della Valpolicella, which was rated 94pts by the Wine Advocate. Normally retailing for $90/bottle or more, the special pricing allows me to offer it to you for $74.99/bottle. Let me put this price into perspective. Before the 2004 came in, I wanted to clear out my remaining cases of the 2003, which was rated 92pts by the Wine Advocate. I sold those bottles for $74.99/bottle. That means we are offering you a higher rated vintage for the same price as the vintage being closed out. SCORE!
All alcohol shipped to NJ is subject to 7% NJ sales tax. We reserve the right to limit quantities while supply lasts. Prices effective for a limited time. Prices in this ad are set by Shoppers Vineyard, Clifton, New Jersey ©2012


text:
Buddhism & Psychology
Wisdom publishes landmark works on the emerging forms of psychoanalysis and therapy born out of the ongoing exchange between Western psychology and Buddhist thought and practice. Our Buddhism and Psychology collection contains works on the psychoanalytic and therapeutic application of Buddhist principles, works that record exchanges between Buddhists and scientists in the emerging field of contemplative science, as well as works on traditional Buddhist psychology. Even a casual reader will be fascinated by the cultural exchange that has proven to be exceptionally fertile ground for both Buddhists and psychologists alike.
Buddhism & Psychology: Books
A Day-by-Day Guide
Enhancing Your Performance, Relationships, Spirituality, and Health
Opt Out of Delusion, Tune In to Truth
A Zen Guide
Transforming Your Anxiety About Impermanence and Death
40 Time-Tested Tools for Creating a More Compassionate Life
How To Heal Your Life and Find Self-Fulfillment
A Buddhist Guide to Breaking Free of Habits
A Journey to the Center
A Mindful Guide


text:
Police are appealing for information after a bus driver was pelted with eggs as he drove through Sabden.
This incident is one of several reports of nuisance recently made by residents living in Sabden.
Clitheroe Police PCSO Di Bioletti is now asking for both parents and local shopkeepers to be on the look out for children taking eggs from home or buying them.
"Myself and PC Jon Scorah are working with our engage team (youth engagement) to try to reduce the nuisance experienced by Sabden residents.
"We would ask parents and shop keepers to look out for youths taking/buying eggs and for parents to be aware of where their youngsters are and what they are doing, especially in the evening.
"The nuisance is being caused by a small minority of young people in the village, but the effect on some residents can be considerable in terms of making them feel vulnerable and intimidated."
Action has already been taken in the case of one of the young people who was involved in some of the previous incidents.
Anyone with information about any of the incidents is asked to call the police by dialling 101.


text:
Who - What
My Artwork
In Progress
Contact Me
Pricing Page
Studstable Company
I'm thinking I might like to build a few of these each year.
Click on picture to see more.
he table is great for Artists and Hobbists who dont have alot of room to practice their craft. Table supports up to 40 pounds and is quite sturdy. One person can asily re-position the table , using the handle and the pinned brackets that slide into various spots up and down the studs that hold it in place. Built as a craft table - not fine furniture.
Contact me if you are interested.
C. Scott Gavin Studios 509-670-9278


text:
Today, I'm so excited to introduce you to Jessica of Midwest Muse! I honestly don't know when I started to follow Jessica because it feels like I always have. She is easily one of my favorite bloggers and I think her style is unparalleled. How she combines feminine and edgy pieces blows my mind, seriously, she's amazing. I gushed about her in a post last week and have to say it again, she's making Ohio look cool. (That's a big thing for a Pittsburgher to say!) She also has a fantastic taste in music, if I do say so myself, considering we like a lot of the same bands. I feel like had we met in high school, we would've been friends to this day. I hope you enjoy meeting her today and checking out her impeccable style.
|All photos provided by Jessica.
How would you define your style?
What are some of your favorite pieces or go-to pieces in your closet?
Where does the inspiration for your outfits come from?
If someone asked you for style advice, what would you tell them?
What advice do you have for bloggers just starting out?
Also I think it's important to note that you don't need fancy cameras, expensive software, designer clothes. Just be you and use what you have. You will learn so much just from blogging. Getting started is the hardest part, but once you do, everything else will eventually happen if you work hard.


text:
Chiefs win at home
After dropping the first two games of the Major Series Lacrosse final, the Chiefs played their most complete game of the series so far, beating the Peterborough Lakers 7-6 in front of over 750 fans at the Iroquois Lacrosse Arena.
The Lakers still lead the best-of-seven final 2-1.
Randy Staats led the way for the Chiefs in the first period, scoring a pair of goals midway through the frame to give his team a one-goal lead after 20 minutes.
Dhane Smith scored the game's first goal of the game, with Shawn Evans and Chad Culp responding for the Lakers.
In the second period, Smith and Kasey Beirnes scored two minutes apart, while Evans and Curtis Dickson continued to supply the Lakers their offence.
Smith scored his third goal of the game, and second on the powerplay early in the third period, while Stephen Keough scored what would be the game-winner with 10 minutes left in regulation.
Dickson and Adam Jones scored for the Lakers.
Brandon Miller regained his form with a fantastic effort in goal for the Chiefs, making 29 saves, while Evan Kirk took the loss in a 33-save effort.
The series resumes Sunday in Peterborough at 7pm, with Game 5 back at the ILA Monday at 8pm.
Six Nations Chiefs v Peterborough Lakers
Lakers lead best-of-seven MSL Final 2-1
Game 1 Peterborough 6, Chiefs 4
Game 2 Peterborough 18, Chiefs 14
Game 3 Chiefs 7, Lakers 6
Game 4 August 23, Peterborough, 7pm
Game 5 August 24, ILA, 8pm
Game 6 August 27, Peterborough, 8pm
Game 7 TBD


text:
Thanks to Guy Wadsworth for wrting up this article.
Flyers heading to the joint RIMRA, CMASS and CATO launch at the URI sod farm field in West Kingston, RI, had to deal with sometimes heavy fog during their drive. Luckily, the fog had lifted by the time setup began.
Fairly low clouds threatened rain for most of the day, but they stayed above the 1500 ft waiver height and we stayed dry. There was a mixture of quite a few first time flyers and even more seasoned flyers, launching a variety of models on motors from 1/4A to J impulse and just about everything in between.
We had 2 flyers try for their Level 1 Certifications, with one making it on the first try and the other needing a second attempt and the aid of some 5 minute epoxy to reattach 2 fins that broke due to a rough landing when the chute release didn't on the first try. There was a notable "Der Red Max" drag race between these two newly certified flyers as well, each flying on G40-7 motors. There were at least 2 flights that overflew the field and ended up on the other side of some trees, and one flight that required the use of the CMASS retrieval pole to recover from the same tree line. The launch was also attended by at least two TARC teams making multiple test flights for the latest TARC competition. Other than the one rather spectacular CATO of a 38mm reload motor that was unfortunately loaded minus its motor casing, most flights went very well and a lot of fun was had by all.
Everyone reported having a good to great time and all the operations ran smoothly.
Thanks to the participants from each club who helped organize this event.
We are calling it the October Sky Launch and hope to make it an annual event.
If you have pictures you'd like to share, please place them in the gallery.


text:
In Sickness and in Wealth is sickeningThis week the OSU College of Public Health presents as part of Public Health Week socio-economic marxist propaganda in a film produced by California Newsreel called, In Sickness and In Wealth, which I mentioned last week I saw on WOSU. It would make Michael Moore proud--my public library will probably buy dozens of copies when it is on DVD. Unbelievably one sided--at least the 10 minutes I saw before turning it off in disgust. The news blurb reports, ". . . state and local public health leaders will participate in a panel discussion, "In Sickness and In Wealth:" at 3 p.m. on Tuesday (4/8) in 160 Meiling Hall, 370 W. 9th Ave. The event, which is part of Ohio State's College of Public Health's celebration of National Public Health Week, is based on a new PBS series called "Unnatural Causes," which explores America's racial and socioeconomic inequities in health. "In Sickness and In Wealth" is the title of the first installment of the series. The episode investigates how a person [sic] a person's work conditions, social status, neighborhood conditions and lack of access to power and resources can actually altar [sic] their human biology and, similar to germs and viruses, make them sick."
Yes, I'm white, middle-class, college educated, married, never collected unemployment, worker's comp or welfare, saved my money, tithed my income, invested in a private pension, had married parents, married grandparents, paid a ton of taxes over my lifetime, purchased private health insurance, kept my weight down, exercised, don't smoke or drink--therefore, I'm causing someone else to be a victim of poor health? I'm altering their biology! They aren't responsible at all! Check out California Newsreel; where do they find these people? California, our proud and loud left coast, of course.


text:
The pulsating corrugator is ideal for the production of large diameter pipe up to 3000 mm.
It features a unique space efficient design and incorporates many of Corma's most innovative technologies such as the in-line coupling, Supercooling™, variable mold block length and compensation for pipe shrinkage.
|Model P 42/120
|Model P 42/120
|Pipe range Min. I.D.
|1000 mm
|Pipe range Min. O.D.
|3000 mm
|Max. line speed*
|0.3 m/min
|1.0 ft/min
|Max. output*
|1500 kg/hr
|3300 lbs/hr
* Line speeds and outputs are theoretical and depend on: pipe diameter; type of plastic; machine model; cooling options; temperature and quantity of cooling water' profile configuration; extruder capacity, etc.
Contact us now for more information.
Contact us now for more information.
The pulsating corrugator is a vacuum forming system which accepts vacuum formable materials including PVC, PE, and PP. It produces double wall pipe with corrugated outer and smooth inner walls for a wide range of applications:
- Transporting aggressive fluids
- Stormwater drains
- Cross drains and culverts
- Sewer pipes


text:
If you're a fan of the Nissan GT-R—and why wouldn't you be—then we've got some good news for you! Shiro Nakamura, Nissan's chief of development, recently shared some morsels of information about the next-generation GT-R. First of all, he confirmed that the next version of the supercar will have a hybrid powertrain. Even more exciting—it will have a front engine setup.
As Nakamura said, the new R36 "will also be a front-engined 2+2 seater coupe" and will "be a hybrid, but not mid-engined." The new GT-R won't just be different on the inside, though; it will have an all-new exterior design as well. Nakamura also confirmed that it will incorporate parts of the Nissan Concept 2020 Vision Gran Turismo concept. However, none of the fan renders of the car online have hit the mark of the new design.
The redesigned Nissan GT-R won't hit the market until at least 2018, but that doesn't keep us from being excited about it here at Blackburn Nissan. In fact, those couple years give Nissan plenty of time to create the best supercar yet. To learn more about the GT-R in all of its configurations—past, current, and future—come see us today!


text:
Advanced Search |
Search Tips
Great looking concert tshirt from the Farewell Tour. Great artwork! These state "Memphis" on the back with past tour dates. Only in men's size Large
We promise to never spam you, and just use your email address to identify you as a valid customer.
This product hasn't received any reviews yet. Be the first to review this product!
Click the button below to add the Farewell Tour I Was There Memphis Mens Tshirt to your wish list.
All prices are in USD
Copyright 2019 KISSArmyWarehouse.com.
Sitemap |


text:
The Canadian Society of Plastic Surgeons is the non profit specialist society that represents the interests of plastic surgeons in Canada. As the voice of plastic surgery in the UK, we aim to raise awareness of the breadth of plastic surgery, to promote innovation in teaching, finding out and analysis and to boost general understanding of the profession. Education models: The Residency Assessment Committee (RRC) for Plastic Surgery recognizes two training models, independent and integrated.
Make confident the plastic surgeon you go to is a member of the American Society of Plastic Surgeons or the American Society for Aesthetic Plastic Surgery You don't want to place your life in someone's hands just to save a thousand dollars. In addition, all of our physicians are also very regarded educators and researchers at the University of Rochester College of Medicine and Dentistry's Division of Plastic Surgery.
Our surgeons specialize in complete solutions for the breast, physique and face — like breast augmentation, breast lift, tummy tuck, body contouring following substantial weight loss, thigh lift, facelift, eyelift, and nose surgery. Aesthetic, or cosmetic, plastic surgery is when somebody wants to modify something about their appearance. Plastic Surgery group does not demand a referral from a main care doctor.
Moreover, during his time at The Plastic Surgery Clinic, he worked to expand his skills as Cosmetic Plastic Surgeon. Plastic surgery has been prominent in the improvement of revolutionary techniques such as microvascular and cranio-maxillofacial surgery, liposuction, and tissue transfer.
Contact us today to discover a lot more or to schedule your private consultation with one of our board-certified plastic surgeons or medical aestheticians. The Customer Research Council of America lists him as one of Americas Prime Surgeons. This site is intended to support potential sufferers identify a nicely-certified and recognized Cosmetic Plastic Surgeon in your geographic region.


text:
September 16th, 2017 8:00am to 5:00pm – Ann Arbor Township Fire Station #2
8 Practical CEU's
This one-day, 8-hour course is being offered FREE and is designed to specifically focus on topics relevant to fire suppression personnel, ie. burn care, fireground trauma and medical, rescue operations of pediatrics and geriatrics, etc. It will additionally provide training to assist fire departments in complying with the practical Continuing Education Unit requirements of Michigan Public Act 368 of 1978, Part 209 – Section 333.20901 – Emergency Medical Services Personnel Licensing.
This course will provide an interactive educational session employing the use of live patient simulators to meet and satisfy the practical requirements in airway/ventilation, medical, trauma, pediatrics and special considerations by utilizing the most modern and nationally accepted approach to patient care.
This class can accommodate up to 32 firefighters. The participants will receive a training certificate, EMS Chit sheet, and supporting educational documentation. The instructors are experienced EMS Instructor Coordinators and fire service certified instructors to better understand the complexities of fire based EMS.
Registration flyer is attached HERE. Deadline to register is September 8th, 2017


text:
Your shopping bag is empty
Sign In My Account
My Account / Sign In
* Required Fields
A new take on the denim jacket, with a softer look and feel. The contemporary collarless style flatters the neck and the diamond quilting adds welcome textural depth. Features include two pockets, panel seaming detail from the shoulders and around the waist, and lustrous mother of pearl buttons. A great layering piece for slightly chillier days, wear over a white linen top and our ponte slim-leg trousers for a modern look.
Chest: S (100cm/ 39") M (110cm/ 43") L (120cm/ 47") XL (132cm/ 52")
Length: 62cm/ 24"
All measurements are approximate.
Colour transference may occur, as a natural characteristic of the fabric.
Code: REJ792-DQD-SS17-DEN
100% Cotton
Hand wash or machine wash inside-out at 30 degrees with similar colours
Iron on a low heat
Dry flat
Please avoid contact with lighter coloured fabrics and upholstery as colour transference may occur.
Colour may fade gradually after washing due to the natural characteristic of the fabric.
Free UK delivery on all orders over £200
UK Standard: £4.95 - delivery within 3-5 working days
UK Next Working Day: £9.95 - Order by 2pm Monday-Thursday for Next Day Delivery
Click here for more information on International and UK delivery times
Your catalogue request has been received and we will send you a free copy of our next brochure as soon as it is available.
Sign up for regular updates on new collections and promotions.
Copyright Sahara Ltd. 2019


text:
TSMC expects to launch 7nm process in 2018 and 5nm in 2020
TSMC expects to launch 7nm process in 2018 and 5nm in 2020
Published: 19th January 2016 | Source: Digitimes |
TSMC expects to launch 7nm process in 2018 and 5nm in 2020
TSMC have some very ambitious goals for the future, telling investors that they expect their market share for current 14nm/16nm production to raise to around 70% in 2016 from 40% in 2015, showing great confidence in their ability to beat Samsung/ Globalfoundries 14nm process technology.
Even more ambitious still is their Co-CEO Mark Liu has told investors that they expect to start production of 7nm chips in the first half of 2018 and plan on creating 5nm chips just 2 years later in 2020. He did not specify if this was early risk production or full volume production, but either will be an impressive jump from their current 16nm FinFET process.
TSMC say that they have been conducting the R&D for their their 5nm process for over a year now and that they have made significant progress.
TSMC expect that their 16nm process nodes will account for more than 20% of the foundries total wafer revenues for this year.
You can join the discussion on TSMC's 7nm and 5nm process nodes on the OC3D Forums.
TSMC expects to launch their 7nm process in 2018 and their 5nm process in 2020. https://t.co/vqaIGHUgVZ pic.twitter.com/f0YkWzD737— OC3D (@OC3D) January 19, 2016
Most Recent Comments
Doesn't mean it'll be out though. They could release it but no products will be out for quite some time after and in limited releases due to yields


text:
John Rampton has sat down and written out 50 Reasons to Start a Business. Each of these points cover something that has crossed every entrepreneur's mind, regardless of their industry.
A few are really positive like passion, leadership, and a solid legacy for future generations. Others see the glass as half-empty. Rather than focusing on the negative, convert it into a positive action. Use this refreshed energy to make your idea happen. If he condensed all 50 reasons into one, he'd say it boils down to having a lightbulb go off.
You'll get to a point where you'd rather try and fail than never try at all. That's when you've reached your entrepreneurial calling. Make sure every action counts. Most new CEOs give up at the first sign of distress but if you have an action plan, your business can prevail. CLICK HERE to subscribe to the Professor Savings YouTube channel to learn everything there is to know about business money management.


text:
Speaking of wood, here is a mini-wallet from Fluffy Co with a wood print. Fits your ID, some credit cards, and a little cash/transit pass, etc. The material is Naugahyde which is an unfortunate disappointment. I want leather! Available at the Fluffy Co website for only $15.00US. The price is what makes Naugahyde so appealing.
Monthly Archives: July 2006
Xylon Wood Frame Bikes
I wonder how these amazing timber framed bicycles from Xylon would stand up to the harsh Asian humidity. I have loads of lovely wooden pieces in my house all of which either require constant care or they suffer from some form of wood rot. These are beautiful and call attention to the whole culture of biking in a creative and innovative way. My favourite is the OLL (pictured).
Xylon Bikes.
Tom Bihn Packing Cubes
I have been using Eagle Creek packing cubes for years to help save space and keep my gear organised when I travel. They are an essential part of my kit. Being the Tom Bihn fanboy that I am I thought it worth mentioning that they have recently released their own packing system. It designed designed specifically to work within the compartments of the Aeronaut but I can't see any reason to purchase them for use in any bag.
The Packing Cubes are available in four styles: Small (Fabric/Mesh $15), End Pocket (Mesh, $15), End Pocket (Fabric $15), and Large (Fabric/Mesh $18).
Available direct from Tom Bihn.
Boombag Speaker Bag for iPod
The Boombag is a great new design concept from GRO design. The bag comes with a built in full range speaker, a rechargeable battery, iPod compartment, and can be controlled via the red lable affixed on the outside . To protect your iPod from the elements the bag is made with durable and waterproof speaker fabric. Not many iPod accessories are designed with the elegance that this bag has. They are currently looking for production partners.
Mobile Power – myPower ALL
Trying to stay mobile with your mobile devices is a definite challenge with the seemingly ever increasing power requirements that these devices introduce. I hate the constant checking of multiple batteries, chargers, and how they all clutter up my bag. While we wait for the next great portable power technology to emerge, there are many companies trying to come up with interim solutions. Tekkeon's attempt is the sleek attractive and awkwardly named myPower ALL – a universal lithium polymer rechargeable battery pack. It's designed to power just about any portable device. The myPower ALL is housed in a black and silver case, it measures 3.28″ x 6.8″, 0.92″ deep and weighs a mere 330g. It ships with a leather travel case, with a belt loop on its backside; an AC power adapter, power cord, a set of 8 starter adapter plugs. They don't have adaptors for every device so you need to check beforehand. Of course like any battery powered solution actual usage dictates it's effectiveness. I'm not convinced that carrying around one big power brick is more convenient than a number of smaller ones. The myPower ALL retails for approx. $120.00US.
JanSport Audiopack – a backpack for your iPod
JanSport has been a staple of the students for years and their new LiveWire line include features for the various electronic devices students (and adults) now carry around full-time. Too many devices I think.
JanSport's Audio Pack, available in black, blue with gray trim, or pink with gray trim, is a traditional bookbag backpack—it doesn't include a laptop compartment or sleeve. The audiopack includes iPod integration, you place your dockable iPod in a zippered, neoprene pouch inside the bag's main compartment. Two plugs—one for your iPod's dock-connector port and the other for its headphone jack—connect your iPod to the bag. Near the top of the right-hand shoulder strap is a standard stereo miniplug, into which you plug your headphones; lower on the strap is a set of soft-button iPod controls: Play/Pause, Volume Up, Volume Down, Forward, and Back. Styling aside I like this product but if you are like me you expect a quality bag to last for years. I wonder if we will still see use from the integrated controls in a few years or even next season. Bags may soon have the same rapid obsolescence as all the gadgets we buy. About $100.00US.
Tom Bihn Imago Messenger Bag
Tom Bihn has recently announced the availability of their Imago Messenger bags. Made of 1000 denier Cordura, the Yin Imago is available in color combinations Cocoa/Cocoa/Wasabi, Plum/Crimson/Steel, and Olive/Steel/Wasabi. The Yang Imago, made of 1050 Ballistic nylon model, has a more conservative look, and is available in color combinations Black/Black/Wasabi, Steel/Kiwi/Deep Blue, and Crimson/Steel/Crimson. We had a sneak preview back in March. When used in conjunction with their Brain Cell insert this becomes the messenger I have always wanted. It's small and perfect for carrying around my 12″ Powerbook (it works with the 13″ MacBook too). Unfortunately Apple has made the unfortunate decision of discontinuing my laptop of choice but that won't hamper my enthusiasm for what is the laptop bag I have been waiting for. It's loaded with useful features all of which are explained in detail on their site. Tom Bin makes great bags (I am a fan) and this bag is worth considering.
Artisan Leather Messenger Bag
Dark brown leather and with solid antiqued-brass hardware make this a messenger that will only get better with age. It's big enough to allow for use as an over-nighter or if your standard heavy mixture of books and gear. The adjustable hardware allow you customise the bag for whatever use you can think of. It's truly a beautiful messenger. $195.00US a J. Crew.


text:
Thinking of selling on eBay and stuck for space..? Do you need storage space in Manchester area for your eBay business? We offer a cost effective self storage option it is ideal for eBay sellers, power sellers or new eBay start ups or Amazon sellers, even shop retailer's benefit from our low cost self storage solution.
If you are thinking of where you will store your stock which would otherwise clutter up your place of residence, our storage facility is ideal for you, open seven days come and ago as you please, store as much stock as you want short term or long term. Conveniently located close to Arndale Manchester.
We can accept deliveries on your behalf at no extra charge, just give us prior notice of when you are expecting a delivery and we will take care of it, our onsite staff will unload it with care and store it in a secure dry place until your arrival.
Free secure car parking area for all customers, monitored with CCTV, 24hours onsite security patrol. Easy to load and unload drive right up to you storage unit,