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Sometime the hardware is not working and we cannot find the reason. Then we need to check the hardware driver. For instance, if our Asus xx driver is corrupted, missing, the hardware cannot work. Then how to update Asus hd7770 2gd5 driver?
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3 Click the "Driver" tab and then click "Update Driver software" to let the computer automatically search for new files. Download the driver and then restart your computer. The new driver should enable the hd7770 2gd5.
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Pan-Seared, Oven-Roasted Thick-Cut Pork Chops
From Perfect Pork
Why This Recipe Works
We knew our pork chop recipe would have to take into consideration today's leaner pork and employ a cooking method that would maximize flavor while preserving moisture. So we brined the pork in a concentrated solution of water, sugar, and salt for a short time, improving flavor and gaining a desirable degree of caramelization during pan-searing. Then we cooked the chops to a fairly low temperature—125 degrees—and allowed them to finish cooking off heat, covered with aluminum foil, while they rested to let the juices redistribute throughout the meat.
Read More

subset: commoncrawl


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Right now, the good ol' US of A is struggling with cross-cultural communication. America is having issues understanding and communicating with the Russians as optimists and pessimists so often do. They're struggling to make sense of the behavior of the honor cultures of the Middle East. And red and blue states have no idea how to work out their differences and take productive action. And yet, the irony is that the solution to all these problems lies in four simple words: Don't Mess With Texas.
Outside of Texas, this phrase became famous when George W. Bush made it one of his signature catchphrases when campaigning for the Presidency of the United States. Will Ferrell then made it even more famous when he made it a core part of his impression of George W. Bush. However, within Texas, Don't Mess With Texas has another meaning; it means don't litter. Check out this ad from 1987 featuring Randy White and Ed "Too Tall" Jones two players for the Dallas Cowboys.
Yep! For the last thirty years, the phrase Don't Mess With Texas has been used to combat littering on Texas highways. Why? Because it works.
You see littering is a choice like any other. You're driving along the highway and you've got some old food wrappers or an empty drink cup sitting in your car with you. What do you do?
Option A: Keep it in your car where it's getting in your way and patiently wait until you get to a trash can.
Option B: Chuck that trash out the window.
Well, which of these two options you pick, depends on your feeeeeeelings about these two choices. If you're this chick, then you're almost certainly picking option A.
She CARES about the planet, man! And the Earth is our mother.
But what if you're this guy?
Well, it was pretty clear to the Texas Department of Transportation that their littering problem overwhelmingly came from one demographic: Bubbas in pickup trucks. That's Texas good ole boys aged 18-35. And so, it hired Mike Blair and Tim McClure to come up with a campaign. They got inside the heads of the people they were trying to reach and come up with a simple slogan that would change their feeeeeeelings about littering. They realized that the key was tapping into Texas Pride.
The campaign was so effective that between 1986 and 1990 it reduced littering on Texas Highways by 72% saving the state huge amounts of money AND helping the environment. Mike Blair and Tim McClure didn't change the choice. They got in the heads of the people they were trying to reach to change how they felt about the choice. As McClure put it, before the Bubbas had felt it was their "God-given right" to litter. Littering was an act of defiance.
The slogan reframed it. Now, NOT littering was an act of Texas Pride. And Texas Pride won out 72% of the time.
From a practical standpoint, there's no difference between "Don't Mess With Texas" and "The Earth is Our Mother Treat Her With Respect." And yet, we feeeeeeel so strongly about these two different slogans that rather than focusing on what practically works, we want it said in OUR way. It becomes about tribe.
Mike Blair and Tim McClure ran into this problem with the Texas Department of Transportation when they pitched the slogan. One of the blue-haired old ladies on the board asked if they could change the slogan to "Please, Don't Mess With Texas." To which McClure responded "No ma'am, you cannot use the line if you put please in front of it."
Cross-cultural communication, bipartisanship and interfaith dialogue are pretty straightforward. You have to take your message and be willing to wrap it in the values and beliefs of the people you're trying to reach. But that means you can't have it your way. Sorry. If you want your red belt in Mixed Mental Arts, you're going to have to get used to learning that sometimes you're not the Burger King.


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What is Herding?
Herding is the ability to let the stock escape -- IN A GROUP -- in the direction in which you would like the stock to go.
In the wild, wolves and wild dogs display herding skills and depend on teamwork in order to hunt.
The teamwork evident in the pack-hunt is the core of the relationship between you and the dog when you are herding.
Herding skills have been developed for centuries in breeds specifically raised to herd other animals and do useful work. After generations of selective breeding for this trait, dogs who have it are said to have "herding instinct".
See more about instinct under Instinct Tests.
Even though herding dogs possess instinct, it still takes training for both dog and handler to make a team. And that's where lessons come in.
Modern herding is done primarily for two reasons -- work and fun.
Depending on your commitment and the frequency of your lessons, you might eventually be interested in training for your first trial. There are various trial venues that you can participate in and Magic's Legacy hosts several trials a year right here.


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Juki MO-6714S/LB6
2-needle Overlock Machine with Semi-automatic Backlatch Device
The Juki MO-6714S/LB6 overlock machine is provided with a back latching capability that eliminates the need for bartacking. The chain-off thread is tucked into the seam at the beginning of sewing to secure chain-off thread and prevent the finished seam from coming undone.
Beautifully finished seams at the beginning of sewing.
Aided by the precise control of the stitch number provided by the material-edge sensor and SC-510 control box, the tension-release mechanism is activated during production of chain-off thread at both the start and end of sewing so that thin chain-off thread can be produced. The chain-off thread is completely tucked into the finished seam on the material to achieve a beautifully finished sewing product.
The machine comes with a throat plate intermediate claw forward/backward mechanism ( for 2-needle overlocking machines).
For the 2-needle overlock machine, the throat plate intermediate claw automatically moves backward at the end of sewing so that the chain-off thread can be guided to the needle thread retainer without getting caught by the claw. At the start of the next sewing, the chain-off thread is rolled into the stitches to the material edge, giving the seam a beautiful finish.
The backlatching function can be released.
The machine is easily adaptable to the standard specifications.
The tension-release function can be disabled by setting the material edge sensor in the OFF state. In addition, for 2-needle machines, the intermediate claw of the throat plate can be fixed with projected. This means that the Juki MO-6714S/LB6 machine can be used as a standard machine with standard specifications for processes not requiring the backlatching capability such as continuous stitching and runstitching, thereby saving on new equipment.
|Model name
|Juki MO-6714S/LB6
|Provided as standard
|Compressed air
|Motor output
|Power requirement
|Single-phase 100120V, 200240V
3-phase 200240V
|Power consumption
|Rating 425VA
For all Industrial Sewing Machine Spare Parts, Needles & Accessories, click the link below to our Parent company, College Sewing Machine Parts Ltd, the UK's largest Industrial Sewing Machine Spare Parts Stockist. Juki Industrial Parts Agents, sewing machine parts for the Juki MO-6714S/LB6
Just fill out the form below with details of the machine on offer and we will see if we can beat or match the price.


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Your success is our success.
As a supply chain solutions provider, our goal is to reduce complexity, increase efficiency, and ultimately reduce costs in your company's supply chain. Aspen arranges for an multitude of cargo shipments for our customers. Our knowledge staff coordinates all shipments from standard van loads to high, wide, and heavy super-loads exceeding 100,000 pounds.
Some of our valued customers


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I've noticed an interesting trend on my visitor stats for the blog. The "Favorite Moment" feature is consistently the most popular. That doesn't say much, but still. Quite a number of you have picked up on my crack-pot routine and check the blog late Friday evening or Saturday morning. In just a few short months you all have learned I could never decide on my favorite moment before the 11th hour.
I actually enjoy deciding on my favorite moment. The process forces me to reflect back on the week and sift through moments I would probably forget were it not for this exercise. My favorite moments are hardly exciting because, quite simply, I lead an exceptionally dull life. To date, I have written about my hair, my dogs, my friends, my drink, and Mickey Mouse. Still, all 5 of you keep coming back and it's either because you keep hoping I'll write about something exciting (I won't) or you, too, appreciate the fulfillment found at 2:37pm on Tuesday when a stranger steps out of line to carry your items to the register just so you can weave your 32 ft long double stroller through the slalom course of sale rounders. (Thanks, 7ft tall African American man at Aaron Brothers! You were freakishly tall and chewed maniacally on a powder-blue floss pick, but that little act of kindness made my day and you, sir, are my favorite moment this week.)
So, I started thinking that it might be nice if all of us made a concerted effort to capture those little fragments in time that we would otherwise forget. William Lyon Phelps once wrote,
See you there!


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Tourism - We Get It!
From small B&Bs to large destination organisations
Getting hold of us couldn't be easier...
Or send us an enquiry using the contact form below and we will get back to you as soon as possible.
* Required
© Copyright Direct Tourism Services.
Tel: 01743 261910
Email: firstname.lastname@example.com
Grove House, 8 St Julian's Friars, Shrewsbury, Shropshire, SY1 1XL


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Meet Our Team
Miller Tanner uses the Strengths Deployment Inventory (SDI) to understand the unique skills, communication styles and leadership abilities of each team member, so we can work together in the most effective fashion and serve our clients in the best way possible. These strengths are represented by the following signature colors:
- Task accomplishment, resource organization, results.
- Flexibility, group welfare, team thinking.
- Well-thought-out approaches, order, individualism.
- Protection, growth, the welfare of others.
- Affirming and developing self-sufficiency in others.
- Intelligent assertiveness, leadership, fairness.
- Protection and growth of others through task accomplishment.
Learn more about our accomplished team members and view their signature colors and corresponding strengths.


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If you didn't see it already, check out this article in the New York Times. It explains well why "demonstrated interest" is one of the new buzzwords in college applications. (And it also reaffirms why I make my students work so hard on their "Why this college?" essays!)
The article also does a great job of showing that there's no real benefit to applying to more than seven or so schools. If you do your research and choose your school well, it will all work out. Don't kill yourself on essays or spend a fortune on applications to schools whose acceptance rate hovers at 10%. Learn to write better (and I'd love to help) and enjoy your senior year!


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Rams, Bucs seeking improvement
TAMPA, Fla. (AP) - St. Louis is clinging to slim playoff hopes, while Tampa Bay is already assured of missing the NFL postseason for the fifth straight year.
Nevertheless, the struggling Buccaneers (6-8) join the improved Rams (6-7-1) in agreeing that both teams have plenty to play for in what's left of seasons under new coaches.
Jeff Fisher has instilled confidence in a team that hasn't earned a postseason berth since 2004 in St. Louis. Greg Schiano had the Bucs in the hunt for a spot until last week's 41-0 loss to New Orleans ended any chance of extending their season for the first time since 2007.
"Coach Fisher's impact has been huge. ... I think one of the biggest things is the overall change in attitude of this team, and of this locker room," Rams quarterback Sam Bradford said of the former Tennessee Titans coach who inherited a team that stumbled to a 2-14 finish last year.
"It's hard to explain. ... But he's such a confident guy and a confident coach. Just watching him, the way he carries himself in meetings, during practice, during the game. Never loses his cool. He's always calm, he's always collected," Bradford added. "It seems like he always knows what his next move is, and he's always very confident in that next move. I think that really carries over to our team and our locker room."
The Rams won three straight, including an overtime victory over NFC West rival San Francisco, to surge into contention after a 3-6-1 start. Last week's 36-22 loss to Minnesota damaged their playoff prospects, however, they can still step a significant step forward by closing strong against Tampa Bay and Seattle.
"Obviously our playoff chances are slim right now, but we can still have a winning season if we win these next two. And, if we beat Seattle ... we can go undefeated in our division," Bradford said. "So there's still a lot left for us to play for. We're still coming with the same attitude we've had all year. This is the most important game of our season right now."
Like St. Louis, Tampa Bay put together a winning streak to rebound from a slow start to get back in the playoff race. The Bucs won four straight to climb to 6-4, but have not played well during a four-game skid that's left them hoping to win the final two games to avoid a losing record.
Josh Freeman threw four interceptions and lost a fumble at New Orleans in one of the worst performances of his career. But the fourth-year pro remains confident the Bucs can pull out of the tailspin and finish on a positive note against the Rams and NFC South rival Atlanta.
Schiano's message to players following last Sunday's blowout loss to the Saints was that they remain united and committed to improving, to weather the adversity.
Freeman said he embraces the challenge of leading the team through hard times.
"Everybody has their own style of leadership, but to the best of my ability I try to convey the message of do your job, sacrifice for your brothers," said Freeman, who's thrown for 25 touchdowns against 12 interceptions.
"In this league nothing's promised, nothing's guaranteed. You have to go out and every week give it all you've got. ... At the end of the year when you look back and reflect, there's going to be a lot of stuff that you look at and say: 'Man, we were that close,"' Freeman added. "At the same time, this is a new team. There are a lot of new pieces. ... You just have to continue to get better. That's the one thing you've got to focus on."
Schiano said he hasn't detected any signs of quit in a young team that's been tested by injuries and suspensions of key players.
"There's a lot of belief in those guys, a lot of togetherness, a lot of 'we're going to do our best.' And that's all you can do is do your best. You do your best and see where that stacks up against your opponent. What gets you is when you don't do your best. That's where you get aggravated and frustrated," Schiano said.
The first-year coach stressed that he will play the final two games to win, not evaluate young players or rest players who've already proven they have futures in the organization.
"We're in the early phases of the development of this program. Part of that is learning how to win and win consistently. So that's my No. 1 and only goal," Schiano said. "This is a win-now league, and this is a win-now coach. We are going to try to win Sunday. That's what we're here to do."


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Rep. Frank Lucas expressed an exhausted sort of gratitude Monday night after he and fellow farm-bill conferees released the final 959-page bill.
In testimony before the House Rules Committee, the Oklahoma Republican argued that the bill is the best possible deal for both parties, at one point even calling it "amazing." After months of work, though, Lucas admitted that he's happiest that the whole process is over, noting that the negotiations around the dairy industry alone were incredibly taxing for negotiators.
"If I expire in the next three days, I want a glass of milk carved on my tombstone — because it's what killed me," he said.


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On October 20, 1619, en route to Jamestown, Virginia, the magazine ship "Warwick" belonging to Earl of Warwick Robert Rich [1587–1658] — reportedly used to fight off the Spanish Armada in 1588 — made a scheduled stop in Bermuda. After completing the first stretch of the voyage it had to re-provision, discharge some of the cargo and passengers, and load valuable products bound for England. "Warwick's" arrival here was as an important event for the island. On that voyage, the ship was charged with delivering Captain Nathaniel Butler, the new Governor of the permanent Bermuda colony founded in 1612.
"Apart from delivering Butler, 'Warwick' was to carry supplies and settlers to the struggling colony at Jamestown and collect colonial products, mostly tobacco, from Bermuda and Virginia for return to England," said marine archaeologists Piotr Bojakowski and Katie Custer.
"At the end of November, as 'Warwick' was preparing to depart for America, where the Jamestown settlers were no doubt eagerly awaiting the arrival of the ship and the supplies it carried, a hurricane struck. Although the crew had prepared the 'Warwick' as the storm approached, all the moorings suddenly gave way and the ferocious wind drove the ship right into the rocky cliffs of Castle Harbour.
"Due to a combination of a powerful north-westerly, shallow reefs, and the sharp limestone rocks surrounding Castle Harbour nothing could be done to save it. 'Warwick's' hull, although sturdy, was no match against the elements; thus, its fate was sealed."
Since 2008 archaeologists from Bermuda, the National Museum of Bermuda, Texas A&M University, the University of Southampton, and the Vasa Museum have dedicated their time, resources and expertise to excavating "Warwick's" wreck.
And now Doug Inglis, assistant director of the "Warwick" Shipwreck Excavation, has concluded Robert Rich may have had more than merchant work in mind when he dispatched the ship to the New World.
Based on the team's discovery of an impressive arsenal of weapons aboard the wreck, Mr. Inglis has concluded "the little vessel may have been armed for more than self defense."
Writing on his blog Diving Archeology recently, Mr. Inglis said:" Sir Robert, the Earl of Warwick, was a major shareholder in the Bermuda Company. Although his stake in the joint-stock company was intended to be an important source of income, Sir Robert involved himself in privateering — legal piracy — on the side.
" … excavation of the shipwreck is revealing a number of exciting clues about the nature of the vessel. I have been part of an international team that has be working on the wreck since 2008.
"One thing that impressed us about the 2011 field season was the variety of ordinance we found aboard the ship. In addition to cannon balls, we found spiked shot, as well as bar shot and expanding bar shot — both designed to de-mast and disable other vessels. Was this offensive armament standard for merchantmen at the time, or was 'Warwick' prepared for more than just evasion?"
Before venturing into underwater archaeology, Mr. Inglis specialised in high-altitude archaeology — working in Rocky Mountain National Park and alpine regions of Colorado and Wyoming.
Aside from his work on the "Warwick" project, Mr. Inglis is an archaeological illustrator at Texas A&M's New World lab. He is completing a Master's thesis in Nautical Archaeology.
At the end of May he will be returning to Bermuda to join other archaeologists from the island and around the world to take part in the final season of the "Warwick" excavation.


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A Preston man, Christian Schauf, was injured April 13 in a one-vehicle rollover accident on NE 50th Avenue.
A Preston man was injured April 13 in a one-vehicle rollover accident in northeast Pratt County.
Christian Schauf, 23 of Preston, was northbound on NE 50th Avenue in a 1998 Chevrolet pickup after just turning off K-61 highway when he lost control. The pickup went off the roadway and overturned in the ditch, according to the Kansas Highway Patrol crash web site.
Pratt County EMS transported Schauf to Pratt Regional Medical Center where he remains in fair condition. Schauf was wearing a seat belt at the time of the accident that was reported at 10:15 p.m.


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- Research article
- Open Access
EST based phylogenomics of Syndermata questions monophyly of Eurotatoria
- Alexander Witek†1,
- Holger Herlyn†2,
- Achim Meyer3,
- Louis Boell4,
- Gregor Bucher4 and
- Thomas Hankeln1Email author
© Witek et al; licensee BioMed Central Ltd. 2008
- Received: 16 June 2008
- Accepted: 29 December 2008
- Published: 29 December 2008
The metazoan taxon Syndermata comprising Rotifera (in the classical sense of Monogononta+Bdelloidea+Seisonidea) and Acanthocephala has raised several hypotheses connected to the phylogeny of these animal groups and the included subtaxa. While the monophyletic origin of Syndermata and Acanthocephala is well established based on morphological and molecular data, the phylogenetic position of Syndermata within Spiralia, the monophyletic origin of Monogononta, Bdelloidea, and Seisonidea and the acanthocephalan sister group are still a matter of debate. The comparison of the alternative hypotheses suggests that testing the phylogenetic validity of Eurotatoria (Monogononta+Bdelloidea) is the key to unravel the phylogenetic relations within Syndermata. The syndermatan phylogeny in turn is a prerequisite for reconstructing the evolution of the acanthocephalan endoparasitism.
Here we present our results from a phylogenomic approach studying i) the phylogenetic position of Syndermata within Spiralia, ii) the monophyletic origin of monogononts and bdelloids and iii) the phylogenetic relations of the latter two taxa to acanthocephalans. For this analysis we have generated EST libraries of Pomphorhynchus laevis, Echinorhynchus truttae (Acanthocephala) and Brachionus plicatilis (Monogononta). By extending these data with database entries of B. plicatilis, Philodina roseola (Bdelloidea) and 25 additional metazoan species, we conducted phylogenetic reconstructions based on 79 ribosomal proteins using maximum likelihood and bayesian approaches. Our findings suggest that the phylogenetic position of Syndermata within Spiralia is close to Platyhelminthes, that Eurotatoria are not monophyletic and that bdelloids are more closely related to acanthocephalans than monogononts.
Mapping morphological character evolution onto molecular phylogeny suggests the (partial or complete) reduction of the corona and the emergence of a retractable anterior end (rostrum, proboscis) before the separation of Acanthocephala. In particular, the evolution of a rostrum might have been a key event leading to the later evolution of the acanthocephalan endoparasitism, given the enormous relevance of the proboscis for anchoring of the adults to the definitive hosts' intestinal wall.
- Ribosomal Protein
- Horizontal Gene Transfer
- Phylogenetic Position
- Tree Reconstruction
- Sister Group Relationship
In the present study we analyse the phylogenetic position of Syndermata within the spiralian clade as well as the phylogenetic relations among the syndermatan subtaxa. We particularly focus on the question whether Eurotatoria are monophyletic and – if not – whether bdelloids or monogononts are more closely related to acanthocephalans. As ribosomal proteins are favorable tools for metazoan molecular phylogenetic analyses [18, 30–32] and easy to obtain from EST libraries, we compiled a phylogenomic dataset comprising 79 ribosomal proteins. To this end, we generated EST libraries for one monogonont (B. plicatilis) and two acanthocephalans (P. laevis and E. truttae; both Echinorhynchida) and sequenced 1,000–2,000 ESTs per library. The new sequences were complemented with ortholog data from public databases for the monogonont B. plicatilis, the bdelloid P. roseola and 25 additional metazoan taxa. Data of Seisonidea have not been included in the present analysis as it is extremely difficult to obtain sufficient material for the preparation of a cDNA library. As a beneficial side effect, the present tree reconstruction cannot be disturbed by the observed long branch leading to representatives of Seisonidea (see, e.g., [19, 24]).
Sequence analyses and ribosomal protein alignment
List of the syndermatan species for which new data have been collected in the present analysis
Pomphorhynchus laevis
Gravel pit at Gimbsheim, Germany
(from host Barbus fluviatilis)
Echinorhynchus truttae
River Leine at Göttingen, Germany
(from host Salmo trutta fario)
Brachionus plicatilis
Lab culture + public data
16 (28)
Philodina roseola
Public data
0 (72)
Syndermatan coverage in the dataset
# amino acids
% of coverage
Pomphorhynchus laevis
Echinorhynchus truttae
Brachionus plicatilis
Philodina roseola
Phylogenetic reconstruction
Results from hypotheses testing
Bdelloidea + Acanthocephala
Bdelloidea + Monogononta (Eurotatoria)
Monogononta + Acanthocephala
The phylogenetic position of Syndermata within Spiralia has been described previously based on molecular data such as 18S rRNA and 16SrRNA [15], 28S rRNA and 18S rRNA [20], ribosomal proteins [18] and on morphological characters like spiral cleavage, filiform sperm without accessory centriole and the subepidermal cerebral ganglion [5]. Likewise, a close phylogenetic relationship of Syndermata and Platyhelminthes within the spiralian clade agrees well with results from previous molecular and morphological approaches on metazoan phylogeny (e.g., [15, 18, 20, 34]). On the other hand, conflicting results from the present tree reconstructions indicate that even the analysis of up to 79 ribosomal proteins from up to 29 species cannot settle the question of the definite phylogenetic relationship of Syndermata and Platyhelminthes. In agreement with Dunn et al. [34] we recommend an enlarged taxon sampling and the incorporation of more closely related groups such as gnathostomulids and micrognathozoans for resolving the question of the sistergroup relationships of Syndermata and Gnathifera, respectively.
In contrast to the still contradictory results regarding the phylogenetic position of Syndermata within Spiralia, our tree reconstructions consistently depict Bdelloidea as more closely related to Acanthocephala than to Monogononta, though with partly moderate support (Fig. 3, 4 and 5). As the moderate support values have been calculated on the basis of the full-length dataset, they might be due to missing data in one or more of the syndermatan lineages sampled. This is at least suggested by the higher statistical support for a clade Bdelloidea+Acanthocephala that could be inferred from the shorter dataset without these missing data (PhyML: 83; Treefinder: 85; PhyloBayes: 0.92). Regardless of differences in the support, analyses of both datasets lead to the same topology among the syndermatan representatives, whichever algorithm was employed. We take this high nodal stability (reflected also by unambiguous results from hypotheses testing) as evidence for reliability of the found grouping of Acanthocephala and Bdelloidea (see [35] for a discussion of nodal stability in the formulation of phylogenetic hypothesis). The future incorporation of EST data from Gnathostomulida and Micrognathozoa, and especially their use as outgroup, will be necessary to yield improved nodal support for the implicit paraphyly of Eurotatoria.
As another note of caution, one has to be aware that the present results as well as consistent results from more limited datasets [24–26] could in principle be influenced by an acceleration of sequence evolution on the branch of the only monogonont sampled, i.e. B. plicatilis. It is thus conceivable that a deviating mode of sequence evolution in B. plicatilis (as described for hsp82 [28]) triggered an attraction of the bdelloid and acanthocephalan branches. On the other hand, this is not very likely as the tree reconstruction methods employed herein (maximum likelihood and bayesian inference) are relatively robust to long-branch attraction. Moreover, Eurotatoria appeared paraphyletic in previous analyses comprising several monogononts [17, 19] which cannot be explained by long-branch attraction due to an accelerated sequence evolution in B. plicatilis. We therefore do not believe that a faster sequence evolution along the B. plicatilis branch is causative for the found clustering of Bdelloidea and Acanthocephala.
The present evidence for a paraphyly of Eurotatoria is in apparent conflict with three out of the five competing hypotheses on the intra-syndermatan phylogeny, i.e. the Eurotatoria+Pararotatoria hypothesis (Fig. 1B), the classical Rotifera+Acanthocephala hypothesis (Fig. 1C), and the Eurotatoria+Acanthocephala hypothesis (Fig. 1D). At first sight, the observed grouping of Bdelloidea and Acanthocephala (under exclusion of Monogononta) rather supports the predictions of the Lemniscea hypothesis of Lorenzen ([23]). However, considering previous evidence from morphological [5, 9, 12] and molecular data [24] as well as from approaches combining both types of data [25, 29], it is still possible that Seisonidea represent the true acanthocephalan sister taxon. However, it cannot be ruled out that Seisonidea are the sistergroup of Bdelloidea, Pararotatoria or Monogononta+Acanthocephala (see single trees in [19, 25]).
Given the uncertain phylogenetic position of Seisonidea within Syndermata, one has to be cautious when inferring the evolution of morphological characters. On the other hand, the well supported closer relation of Bdelloidea to Acanthocephala, with exclusion of Monogononta ([19], present study), allows for some conclusions regarding the evolution of morphological characters that are not bound to the position of Seisonidea within Syndermata. It is thus very likely that the rotatory organ or corona underwent a (partial or total) reduction before the separation of Acanthocephala. A likewise reduction of a newly emerged character (wings) has for example been described in stick insects (Phasmatodea; [36]). Therefore the reduction of the rotatory organ only a few splits after its emergence at the base or within the syndermatan tree is not as unlikely as it might appear at first sight. Another implication of the grouping of Acanthocephala and Bdelloidea is that a retractable anterior end – whether in the shape of a rostrum in Bdelloidea or as a hooked proboscis in Acanthocephala – probably evolved before the separation of the acanthocephalan stem lineage as well. The reduction of the corona as well as the evolution of a retractable anterior end can easily be explained by different life-styles and patterns of locomotion in the syndermatan subtaxa: free living/free swimming in Monogononta; leech-like creeping/free living in Bdelloidea; leech-like creeping/epibiontic in Seisonidea; reduced motility/endoparasitic in Acanthocephala (see also [24, 37–39]). Particularly the early evolution of a retractable anterior end might have represented a key event leading to the later evolution of the acanthocephalan endoparasitism, given the crucial role of the proboscis in the anchoring of adult acanthocephalans to the definitive hosts' intestinal wall [40].
Based on a dataset comprising sequences from up to 79 ribosomal proteins of up to 29 species, we provide evidence for the paraphyly of Eurotatoria. Irrespective of the tree reconstruction method and dataset used (and additionally supported by hypothesis testing) we found Bdelloidea to be more closely related to Acanthocephala than to Monogononta. Although data for Seisonidea have not been included in the dataset, the present findings allow for the rejection of three (Eurotatoria+Pararotatoria, Eurotatoria+Seisonidea, Eurotatoria+Acanthocephala) out of the presently five competing hypothesis regarding the phylogeny within Syndermata. On the other hand, additional data are needed to determine the actual acanthocephalan sistergroup (Seisonidea or Bdelloidea). Irrespective of these limitations it is very likely that a (partial or complete) reduction of the rotatory organ or corona occurred before the separation of Acanthocephala. Likewise, a retractable anterior end most likely emerged before the separation of the acanthocephalan stem lineage. Considering the importance of the proboscis for the attachment of acanthocephalans to the definite host's intestinal wall, the latter step can be regarded as a key event towards the evolution of acanthocephalan endoparasitism.
Isolation of RNA and cDNA library construction
Total RNA was extracted from frozen pooled specimen using column-based methods (Qiagen RNeasy Plant Mini Kit, Qiagen, Hilden, Germany). Quality of RNA was visually checked on agarose gels and mRNA was subsequently captured using the NucleoTrap mRNA kit (Macherey-Nagel, Düren, Germany) for B. plicatilis and the polyATract mRNA Isolation System III (Promega, Mannheim, Germany) for P. laevis and E. truttae. cDNA libraries were constructed at the Max Planck Institute for Molecular Genetics in Berlin (P. laevis) and the Institute of Molecular Genetics, University of Mainz (E. truttae, B. plicatilis) by primer extension (P. laevis, B. plicatilis) or LD-PCR (E. truttae), size fractionation and directional cloning applying the Creator SMART cDNA Libraries Kit (Clontech, Heidelberg, Germany) with the vectors pDNR-LIB or a modified pSPORT [41]. Clones containing cDNA inserts were sequenced from the 5' end on ABI 3730 capillary sequencer systems using BigDye chemistry (Applied Biosystems, Darmstadt, Germany).
EST processing
EST processing for P. laevis was accomplished at the Center for Integrative Bioinformatics in Vienna. Sequence chromatograms were first base-called and evaluated using the Phred application [42]. Vector, adaptor, poly-A tract and bacterial sequences were removed employing the software tools Lucy http://www.tigr.org, SeqClean http://compbio.dfci.harvard.edu/tgi/software, and CrossMatch http://www.phrap.org, respectively. Clustering and assembly of the clipped sequences was performed using the TIGCL program package http://compbio.dfci.harvard.edu/tgi/software by performing pairwise comparisons (MGIBlast) and a subsequent clustering step (CAP3). Low quality regions were then removed by Lucy. Finally, contigs were tentatively annotated by aligning them pairwise with the 25 best hits retrieved from NCBI's non-redundant protein database using the BlastX algorithm http://www.ncbi.nlm.nih.gov. Alignment and computation of the resulting match scores, on which the annotation was based, were conducted by GeneWise [43] in order to account for frame shift errors.
ESTs for E. truttae and B. plicatilis were processed semi-automatically: removal of vector parts, polyA tails and bad quality sequence from sequence traces was performed by the SeqMan option of the DNASTAR program suite (Lasergene). Overlapping EST sequences were clustered using SeqMan (Lasergene). For B. plicatilis publicly available data from dbEST and the trace archives were included into the clustering process, and public data for Philodina roseola was clustered the same way. For annotation, EST cluster consensus sequences and EST singletons were subjected to BLASTX comparison against the SWISS-PROT protein database at NCBI http://www.ncbi.nlm.nih.gov/, using a BLAST client tool (Blastcl3, Blast software package, NCBI) setting the cut-off to 1*e-10. The EST data used in our analyses have been deposited in Genbank under the accession numbers [GenBank: AM849482 – AM849546 (P. laevis), AM980962 – AM980984 (E. truttae) and AM980946 – AM980961 (B. plicatilis)].
Sequence analysis and ribosomal proteins alignment
Ribosomal protein sequences were extracted from the newly obtained and publicly available EST data by their annotation. EST sequence contigs were checked for assembly errors by visual inspection and by comparison with corresponding sequences of related taxa, and translated into amino acid sequences. Gladyshev et al. [33] recently reported evidence for gene aquisition by horizontal gene transfer in two bdelloid species. Although it is unlikely that ribosomal proteins are subject to horizontal gene transfer, but as a precaution, we checked whether our data are influenced by horizontal gene transfer or not. Therefore we performed Blastp searches with our amino acid sequences and calculated the 'Alien Index' as introduced by Gladyshev et al. [33]. This index represents a measure of the orders of magnitude by which the BLAST E-value for the best metazoan hit differs from that for the best non-metazoan hit [33]. Additional ribosomal protein data were retrieved from the alignments compiled by Hausdorf et al. [18]. All ribosomal protein sequences obtained were aligned by the ClustalW algorithm using default parameters [44]. The resulting ribosomal protein alignments were inspected and adjusted manually for obviously misaligned positions using GeneDoc [45]. Questionably aligned positions were eliminated with GBlocks [46] using less stringent parameters. To test for the effect of missing data on present results [47], we assembled an additional dataset (24 ribosomal proteins, 3,535 amino acids) from which ribosomal protein sequences without acanthocephalan, bdelloid and/or monogonont orthologs were removed.
Phylogenetic reconstruction
The content of phylogenetic information of the alignments was estimated by the likelihood mapping approach as implemented in Tree-Puzzle 5.2 [48, 49], testing all 23,751 possible quartets with exact parameter estimation.
Bayesian inference analyses based on the site-heterogenous CAT model (which allows the amino-acid replacement pattern to vary across a protein alignment; [50]) were performed using PhyloBayes v2.1c [51]. Two independent chains were run simultaneously for 11,210 points each. Chain equilibrium was estimated by plotting the log-likelihood and the alpha parameter as a function of the generation number. The first 500 points were subsequently discarded as burn-in. According to the divergence of bipartition frequencies, both chains reached convergence (maximal difference <0.08, mean difference <0.003), supported by the fact that both chains produced the same consensus tree topology. Taking every 10th sampled tree, a 50% majority rule consensus tree was finally computed using both chains.
ProtTest [52] was used to assess the appropriate model of sequence evolution for maximum likelihood-based tree reconstruction. As ribosomal proteins are likely to evolve similarly, the model was determined for the concatenated dataset, instead of for each single protein. Analyses were then conducted using PhyML [53] and Treefinder [54, 55] with the rtREV+I+G+F substitution model [56] and 500 bootstrap replicates. Confidence values for the edges of the maximum likelihood tree (Treefinder) were computed by applying expected likelihood weights (ELWs) [57] to all local rearrangements of tree topology around an edge (1,000 replications). Trees produced in the course of the analysis were further edited using TreeView [58].
To test predefined phylogenetic hypotheses, we used constrained trees and the 'resolve multifurcations' option of Treefinder to obtain the maximum likelihood tree for a specified hypothesis. Thereafter we investigated whether the maximum likelihood trees for these hypotheses are part of the confidence set of trees applying the expected likelihood weights method [57].
We gratefully acknowledge the work of Michael Kube and Richard Reinhardt (Max Planck Institute for Molecular Genetics, Berlin) in cDNA library construction and sequencing, Ingo Ebersberger, Sascha Strauß and Arndt von Haeseler (Max F. Perutz Laboratories, Center for Integrative Bioinformatics, Vienna) in processing ESTs in the framework of the DFG priority programme SPP1174 "Deep Metazoan Phylogeny", David Mark Welch and two anonymous referees for their helpful comments on the earlier version of this manuscript and Ana Rogulja-Ortmann for language editing. The work was funded by the Deutsche Forschungsgemeinschaft (DFG grant Ha 2103/4-1, SPP1174).
Authors' Affiliations
- Westheide W, Rieger R: Spezielle Zoologie, Teil 1. Einzeller und wirbellose Tiere. 1996, Stuttgart, Jena, NewYork: Verlag Gustav FischerGoogle Scholar
- Ricci C: Are lemnisci and proboscis present in Bdelloidea?. Hydrobiologia. 1998, 387–388 (4): 93-96.View ArticleGoogle Scholar
- Segers H: Annotated checklist of the rotifers (Phylum Rotifera), with notes on nomenclature, taxonomy and distribution. Zootaxa. 2007, 3-104. 1564Google Scholar
- Sørensen MV, Segers H, Funch P: On a new Seison Grube, 1861 from coastal waters of Kenya, with a reappraisal of the classification of the Seisonida (Rotifera). Zoological Studies. 2005, 44 (1): 34-43.Google Scholar
- Ahlrichs WH: Ultrastruktur und Phylogenie von Seison nebaliae (Grube 1859) und Seison annulatus (Claus 1876). Hypothesen und Verwandschaftsverhältnisse innerhalb der Bilateria. 1995, Göttingen: CuvillierGoogle Scholar
- Wallace RL, Colburn RA: Phylogenetic-Relationships within Phylum Rotifera – Orders and Genus Notholca. Hydrobiologia. 1989, 186: 311-318.View ArticleGoogle Scholar
- Meyer A: Acanthocephala. Bronns Klassen und Ordnungen des Tierreichs 4 (2/2). Edited by: Bronn HG. 1933, Leipzig: Akademische Verlagsgesellschaft mbH, 582-Google Scholar
- Wallace RL, Ricci C, Melone G: A cladistic analysis of pseudocoelomate (aschelminth) morphology. Invertebrate Biology. 1996, 115 (2): 104-112.View ArticleGoogle Scholar
- Ahlrichs WH: Epidermal ultrastructure of Seison nebaliae and Seison annulatus, and a comparison of epidermal structures within the Gnathifera. Zoomorphology. 1997, 117 (1): 41-48.View ArticleGoogle Scholar
- Herlyn H, Ehlers U: Organisation of the praesoma in Acanthocephalus anguillae (Acanthocephala, Palaeacanthocephala) with special reference to the muscular system. Zoomorphology. 2001, 121 (1): 13-18.View ArticleGoogle Scholar
- Herlyn H, Rohrig H: Ultrastructure and overall organization of ligament sac, uterine bell, uterus and vagina in Paratenuisentis ambiguus (Acanthocephala, Eoacanthocephala) – the character evolution within the Acanthocephala. Acta Zoologica. 2003, 84 (3): 239-247.View ArticleGoogle Scholar
- Ahlrichs WH: Spermatogenesis and ultrastructure of the spermatozoa of Seison nebaliae (Syndermata). Zoomorphology. 1998, 118 (4): 255-261.View ArticleGoogle Scholar
- Melone G, Ricci C, Segers H, Wallace RL: Phylogenetic relationsships of phylum Rotifera with emphasis on the families of Bdelloidea. Hydrobiologia. 1998, 387–388: 101-107.View ArticleGoogle Scholar
- Garey JR, Near TJ, Nonnemacher MR, Nadler SA: Molecular evidence for Acanthocephala as a subtaxon of Rotifera. Journal of Molecular Evolution. 1996, 43 (3): 287-292.View ArticlePubMedGoogle Scholar
- Garey JR, Schmidt-Rhaesa A, Near TJ, Nadler SA: The evolutionary relationships of rotifers and acanthocephalans. Hydrobiologia. 1998, 388: 83-91.View ArticleGoogle Scholar
- Giribet G, Distel DL, Polz M, Sterrer W, Wheeler WC: Triploblastic relationships with emphasis on the acoelomates and the position of Gnathostomulida, Cycliophora, Plathelminthes, and Chaetognatha: A combined approach of 18S rDNA sequences and morphology. Systematic Biology. 2000, 49 (3): 539-562.View ArticlePubMedGoogle Scholar
- Giribet G, Sørensen MV, Funch P, Kristensen RM, Sterrer W: Investigations into the phylogenetic position of Micrognathozoa using four molecular loci. Cladistics-the International Journal of the Willi Hennig Society. 2004, 20 (1): 1-13.View ArticleGoogle Scholar
- Hausdorf B, Helmkampf M, Meyer A, Witek A, Herlyn H, Bruchhaus I, Hankeln T, Struck TH, Lieb B: Spiralian phylogenomics supports the resurrection of Bryozoa comprising Ectoprocta and Entoprocta. Mol Biol Evol. 2007, 24 (12): 2723-2729.View ArticlePubMedGoogle Scholar
- Garcia-Varela M, Nadler SA: Phylogenetic relationships among Syndermata inferred from nuclear and mitochondrial gene sequences. Mol Phylogenet Evol. 2006, 40 (1): 61-72.View ArticlePubMedGoogle Scholar
- Passamaneck Y, Halanych KM: Lophotrochozoan phylogeny assessed with LSU and SSU data: Evidence of lophophorate polyphyly. Mol Phylogenet Evol. 2006, 40 (1): 20-28.View ArticlePubMedGoogle Scholar
- Sørensen MV, Funch P, Willerslev E, Hansen AJ, Olesen J: On the phylogeny of the Metazoa in the light of Cycliophora and Micrognathozoa. Zoologischer Anzeiger. 2000, 239: 297-318.Google Scholar
- Herlyn H, Ehlers U: Ultrastructure and function of the pharynx of Gnathostomula paradoxa (Gnathostomulida). Zoomorphology. 1997, 117 (3): 135-145.View ArticleGoogle Scholar
- Lorenzen S: Phylogenetic aspects of pseudocoelomate evolution. The origins and relationships of lower invertebrates. Edited by: Conway Morris S, George JD, Gibson R, Platt HM. 1985, Oxford: Clarendon Press, 210-223.Google Scholar
- Herlyn H, Piskurek O, Schmitz J, Ehlers U, Zischler H: The syndermatan phylogeny and the evolution of acanthocephalan endoparasitism as inferred from 18S rDNA sequences. Mol Phylogenet Evol. 2003, 26 (1): 155-164.View ArticlePubMedGoogle Scholar
- Zrzavy J: The interrelationships of metazoan parasites: a review of phylum- and higher-level hypotheses from recent morphological and molecular phylogenetic analyses. Folia Parasitologica. 2001, 48 (2): 81-103.View ArticlePubMedGoogle Scholar
- Nielsen C: Animal Evolution. Interrelationships of the Living phyla. 1995, Oxford: Oxford University PressGoogle Scholar
- Garcia-Varela M, de Leon GPP, de la Torre P, Cummings MP, Sarma SSS, Laclette JP: Phylogenetic relationships of Acanthocephala based on analysis of 18S ribosomal RNA gene sequences. Journal of Molecular Evolution. 2000, 50 (6): 532-540.PubMedGoogle Scholar
- Mark Welch DB: Evidence from a protein-coding gene that acanthocephalans are rotifers. Invertebrate Biology. 2000, 119 (1): 17-26.View ArticleGoogle Scholar
- Sørensen MV, Giribet G: A modern approach to rotiferan phylogeny: Combining morphological and molecular data. Mol Phylogenet Evol. 2006, 40 (2): 585-608.View ArticlePubMedGoogle Scholar
- Philippe H, Snell EA, Bapteste E, Lopez P, Holland PW, Casane D: Phylogenomics of eukaryotes: impact of missing data on large alignments. Mol Biol Evol. 2004, 21 (9): 1740-1752.View ArticlePubMedGoogle Scholar
- Hughes J, Longhorn SJ, Papadopoulou A, Theodorides K, de Riva A, Mejia-Chang M, Foster PG, Vogler AP: Dense taxonomic EST sampling and its applications for molecular systematics of the Coleoptera (beetles). Mol Biol Evol. 2006, 23 (2): 268-278.View ArticlePubMedGoogle Scholar
- Struck TH, Fisse F: Phylogenetic position of Nemertea derived from phylogenomic data. Mol Biol Evol. 2008Google Scholar
- Gladyshev EA, Meselson M, Arkhipova IR: Massive horizontal gene transfer in bdelloid rotifers. Science. 2008, 320 (5880): 1210-1213.View ArticlePubMedGoogle Scholar
- Dunn CW, Hejnol A, Matus DQ, Pang K, Browne WE, Smith SA, Seaver E, Rouse GW, Obst M, Edgecombe GD, et al: Broad phylogenomic sampling improves resolution of the animal tree of life. Nature. 2008, 452 (7188): 745-749.View ArticlePubMedGoogle Scholar
- Giribet G: Stability in phylogenetic formulations and its relationship to nodal support. Systematic Biology. 2003, 52 (4): 554-564.View ArticlePubMedGoogle Scholar
- Whiting MF, Bradler S, Maxwell T: Loss and recovery of wings in stick insects. Nature. 2003, 421 (6920): 264-267.View ArticlePubMedGoogle Scholar
- Near TJ, Garey JR, Nadler SA: Phylogenetic relationships of the Acanthocephala inferred from 18S ribosomal DNA sequences. Mol Phylogenet Evol. 1998, 10 (3): 287-298.View ArticlePubMedGoogle Scholar
- Herlyn H: Zur Ultrastruktur, Morphologie und Phylogenie der Acanthocephala. 2000, Berlin: Logos VerlagGoogle Scholar
- Near TJ: Acanthocephalan phylogeny and the evolution of parasitism. Integrative and Comparative Biology. 2002, 42 (3): 668-677.View ArticlePubMedGoogle Scholar
- Taraschewski H: Host-parasite interactions in acanthocephala: a morphological approach. Advances in Parasitology. 2000, 46: 1-179.View ArticlePubMedGoogle Scholar
- Wellenreuther R, Schupp I, Poustka A, Wiemann S: SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones. BMC Genomics. 2004, 5 (1): 36-PubMed CentralView ArticlePubMedGoogle Scholar
- Ewing B, Hillier L, Wendl MC, Green P: Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res. 1998, 8 (3): 175-185.View ArticlePubMedGoogle Scholar
- Birney E, Clamp M, Durbin R: GeneWise and Genomewise. Genome Res. 2004, 14 (5): 988-995.PubMed CentralView ArticlePubMedGoogle Scholar
- Thompson JD, Higgins DG, Gibson TJ: Clustal-W – Improving the Sensitivity of Progressive Multiple Sequence Alignment through Sequence Weighting, Position-Specific Gap Penalties and Weight Matrix Choice. Nucleic Acids Research. 1994, 22 (22): 4673-4680.PubMed CentralView ArticlePubMedGoogle Scholar
- Nicholas KB, N HB, Deerfield DWI: GeneDoc: Analysis and Visualization of Genetic Variation. EMBNEWNEWS. 1997, 4: 14-Google Scholar
- Castresana J: Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol. 2000, 17 (4): 540-552.View ArticlePubMedGoogle Scholar
- Hartmann S, Vision TJ: Using ESTs for phylogenomics: Can one accurately infer a phylogenetic tree from a gappy alignment?. Bmc Evolutionary Biology. 2008, 8:Google Scholar
- Schmidt HA, Strimmer K, Vingron M, von Haeseler A: TREE-PUZZLE: maximum likelihood phylogenetic analysis using quartets and parallel computing. Bioinformatics. 2002, 18 (3): 502-504.View ArticlePubMedGoogle Scholar
- Strimmer K, von Haeseler A: Likelihood-mapping: a simple method to visualize phylogenetic content of a sequence alignment. Proc Natl Acad Sci USA. 1997, 94 (13): 6815-6819.PubMed CentralView ArticlePubMedGoogle Scholar
- Lartillot N, Philippe H: A Bayesian mixture model for across-site heterogeneities in the amino-acid replacement process. Mol Biol Evol. 2004, 21 (6): 1095-1109.View ArticlePubMedGoogle Scholar
- Blanquart S, Lartillot N: A Bayesian compound stochastic process for modeling nonstationary and nonhomogeneous sequence evolution. Mol Biol Evol. 2006, 23 (11): 2058-2071.View ArticlePubMedGoogle Scholar
- Abascal F, Zardoya R, Posada D: ProtTest: selection of best-fit models of protein evolution. Bioinformatics. 2005, 21 (9): 2104-2105.View ArticlePubMedGoogle Scholar
- Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol. 2003, 52 (5): 696-704.View ArticlePubMedGoogle Scholar
- Jobb G: TREEFINDER. 2007, Available from Munich, distributed by the author athttp://www.treefinder.deGoogle Scholar
- Jobb G, von Haeseler A, Strimmer K: TREEFINDER: a powerful graphical analysis environment for molecular phylogenetics. Bmc Evolutionary Biology. 2004, 4:Google Scholar
- Dimmic MW, Rest JS, Mindell DP, Goldstein RA: rtREV: an amino acid substitution matrix for inference of retrovirus and reverse transcriptase phylogeny. J Mol Evol. 2002, 55 (1): 65-73.View ArticlePubMedGoogle Scholar
- Strimmer K, Rambaut A: Inferring confidence sets of possibly misspecified gene trees. Proc Biol Sci. 2002, 269 (1487): 137-142.PubMed CentralView ArticlePubMedGoogle Scholar
- Page RD: TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci. 1996, 12 (4): 357-358.PubMedGoogle Scholar
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It prompted quite a few interesting comments. The second most popular post was on, believe it or not, neo-Stoicism:
Actually, it was more about the ubiquity of pop culture and the idea of detaching oneself from it. As a musical metaphor I put in a Bruckner symphony. Here is another one, the Symphony No. 8 with the Munich Philharmonic conducted by Celibidache:
The most popular post in April with a considerable number of views and comments, was on some radical ideas about music education.
I think my high point in the post came with my reductio ad absurdum of the author's claim that learning to read music notation is over-rated:
If things keep going in the direction they have, then in a few years I fully expect someone to be opining in the Guardian that the idea of requiring students to read at all is unnecessary. English is, after all, a cryptic, tricky language and learning how to read it largely unneeded now that we have audible books. If you are creative, just dictate your novel into your iPad or iPhone. Really, what is the point of requiring anyone to read written languages?The second most popular post was a very brief one just drawing out attention to the very young soprano Patricia Janečková:
Heck, let's listen to another of her performances. This is the aria "Ombra mai fu" by Handel:


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Welcome to our great new September Sketch challenge this month
created by SKETCH DT Bev Code
Can't wait to see what you will create and share.
Our fabulous sponsor this month is one of our own DT....
Karrie Sweet personal stash prize - Halloween goodie bag - Sept 2017
Now moving on.. here is the amazing creative projects from
our design team to inspire you using the sketch.
Here's Maven Elisabetta's amazing layout. Don't you just love all the ink and fun details she's added? I love that she left a clean edge too, it really makes the fun elements pop.
Thanks for joining us.
Be sure to enter your link by Sept 30, 2017 for your chance to win!
We hope you'll join us and play along using the sketch above.
Add your project to the inlinkz so we can visit and leave comments.
Happy Scrapping,


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An iconic British brand with a heritage of high-quality craftsmanship that dates back to its foundation in Northampton in 1675, Church's has made a name for itself as the go-to name for expertly-made footwear for both men and women.
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Related Products
Featuring the technical capabilities that you'll need to keep you functioning in the field this jacket has rugged style that complements your other outdoor garments. :: Men > Jackets. Body is lined with pile fleece for extra protection against the coldGusseted underarms and articulated elbows enable your full range of natural motion10-oz 100% organic cotton canvas fabric is comfortable to wear and durableWeight: 1.156g (40.8 oz)Full-length zip-fastening at the frontMen's hooded jacket70% recyled fabricDrawstring-adjustable hood with polyester fleece lining protects you from the elements Size: X-Large,Medium.


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Sly Fox is a signature line designed for athletes who see themselves as authentic, like Dave Schultz. He was respected by fans worldwide for his unique, authentic, and original approach to man's oldest and greatest sport. RUDIS continues to evolve as a brand for that select athlete who pushes the limits of the human body and reaches deep within their soul to achieve their potential. Sly Fox is a top flight expression for this difficult to define group of people.
The Sly Fox Authentic Youth Pullover Hoodie is the RUDIS extension of Dave Schultz's unique and rare Way Of Life
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"A show for the ages... a score full of sophisticated comedy numbers that would make Cole Porter proud... the lyrics are so cleverly constructed, the rhyme schemes so elegantly intricate, and the messages so rich and ripe that they make a mockery of most of today's Broadway show scores." – TheaterMania.com
Miss Gulch Returns! is my musical comedy valentine to the romantically disenfranchised, who can find a new metaphorical spokesperson in Miss Gulch, the ultimate "spinster" (as they used to be known). In this "musical vivisection of a stereotype," a contemporary sophisticate trades identities with the infamous curmudgeon, now liberated from her two-dimensional, sepia-toned moorings on the screen. She wrestles with her old-Hollywood stereotype in her new, unlikely profession – cabaret entertainer in a Judy-Garland-obsessed world.
Miss Gulch Returns! began as a five-minute cabaret turn. The full show premiered in January, 1984, to national press, winning a 1985 Back Stage Bistro Award for Musical Comedy Performance. Miss Gulch Returns! played long runs in New York's cabarets, followed by engagements in cities around the country.
The 1986 recording remains a popular seller, over twenty years after its initial release. Miss Gulch's song "Pour Me A Man" is now a cabaret standard, sung on three continents. The show has received award-winning, critically-acclaimed productions in regional theatres around the US and England, and I presented a revised 20th Anniversary production in New York in 2004.
Miss Gulch is me in a bad mood. I wrote the songs because I hear so many gushy love songs everywhere: "I love you," "I'm glad you love me," "you used to love me," "I wish you still loved me," on and on and I thought, how about some songs for single people who haven't found someone to sing gushy love songs about in the first place? Poor Miss Gulch, trapped in that starchy old stereotype as the old-Hollywood spinster – what if she got a chance to let down that iron hairdo of hers and let loose with a ditty or two in a cabaret? Voila – Miss Gulch Returns! "I'm A Bitch" is her theme song – Miss Gulch knows who she is and she loves every minute of it. Almost. "Pour Me A Man" is the big comedy number something Fats Waller might have written if he were a big old lady sitting in a bar without a date. She can have every drink the bartender knows how to make, but who would sit drinking in a bar if the bartender could pour her (or him, depending on who you are) a swell fellow (or whatever you're into) to take home and canoodle with?
But the big song is "Everyone Worth Taking's Been Taken." Ever been single and surrounded by happy couples at a party? Or looked at the Wedding Announcements page in the New York Times? How dare happy people ram their happiness in our faces like that! If they want to be happy, that's their own private business, but do they have to rub our faces in it? That's the Miss Gulch in all of us – the only stimulation she gets is from a bicycle. Here are two of my favorite reactions I've gotten from the show. A young, apparently single woman came up to me afterwards and said, with the faintest tear in her eye, "How did you know what it's like?" "Believe me," I answered, "I know what it's like." On another occasion, after the show a fellow said to me, "I've never seen anyone put on such a ridiculous get-up and become so naked on stage." That got me right where I live (I wonder if they got that comment down at Naked Boys Singing.)
(from the original LP liner notes)
Wrong – she is not Auntie Mame's secretary (that was Agnes Gooch). She is, of course, the dog-snatching, bicycle-riding, spiteful spinster-next-door who had it in for Dorothy's little Toto. Of all Hollywood's ornery old maids, crotchety battle-axes, and crusty curmudgeons, Miss Gulch is the unquestioned queen; she is the ultimate Hollywood stereotype. Pity her! Born to higher things, why was she sentenced to serve forever as two-dimensional comic relief? And why should her formidable figure invariably inspire hoots and hilarity? Perhaps today's sophisticates are amused by quaint Hollywood psychology, in which a severe Miss Gulch becomes a terrifying Wicked Witch in the subconscious mind of a pubescent Midwestern teenager. Or is it possible that we prefer the safety of identifying with victimized superstar heroines; that the startling image of the underdog supporting-player's sexually frustrated spinster hits a deep nerve of unsettling self-recognition that can acknowledge only with laughter?
Poor Almira! For fifty years she has intrigued us, but never got to give us her side of story. She has remained misunderstood, underestimated, and unappreciated. And through the decades, frustrated fans have hungered for solutions to the mysteries surrounding the legendary Miss Gulch. Why does she ride that bike? What does she have against neighborhood girls and their little dogs too? What becomes of her after the tornado? Did she have a happy childhood? And most of all – who is the real woman beneath that ferocious facade?
Enter Fred Barton, budding composer-lyricist-author-actor-singer-musician, armed with a penchant for triple entendres, triple rhymes, honky-tonk rhythms, and determined to revive the long-dormant special-material comedy song tradition. In Miss Gulch, he finds the ideal icon for today's disillusioned souls, the perfect symbol of the frustrated aspirations of our age. He furnishes himself with an armful of devastating new songs, a tastefully outrageous black dress, a wig, a hat, a wicker basket, a grand piano, and voila: Miss Gulch Returns! And Mr. Barton does the old girl proud. All her dreams finally come true: long-running New York engagements rife with raves, guest appearances in cities from coast to coast... a cult following of her own... and, of course, the album!
The Bitch is back in all the glory Hollywood denied her. She sings (at last!). She plays a mean piano (and what other kind?) She gives us the dirt. She dumps Hollywood and gives the real world a try – and lands a job as well as the next man (and lands the next man, as well). She lays her soul bare, and yours if you dare. So turn on the phone machine, turn Judy's picture to the wall, turn up the volume, and welcome to the wit and wisdom of Miss Gulch – "Entertainesse Extraordinaire!"
Read more about the history of Miss Gulch Returns!


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Every time you hear, "studies have shown" you know you're about to learn something ridiculous! Studies have shown your dog is happier than your cat! Who cares! How about they give funding to scientists to come up with a way to eradicate the unibrow or those weird thin white hairs you occasionally find attached to your face!
Let's get some research done on what happens to your blood pressure when your kid keeps getting out of bed when you've tucked them in 20 fu*king times already!


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Brand new and full furnished 1 bedroom apartment for rent in Vinhomes Gardenia
Register this property
- Washing machine
- Air Conditioner
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- Wood floor
- Fridge
- Microwave
- Washing machine
- Television
- Sofa
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- Front Yard
- Balcony
- Near Park
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- Neighbor
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- Elevator
- Internet
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Furnished 2 bedroom apartment for rent in Vinhomes Gardenia
Using Area (m2)
$800/ month
Splendid and full furniture 3 bedroom apartment for rent in Vinhomes Gardenia, Ham Nghi
Using Area (m2)
$1,000/ month
Brand new and full furnished 2 bedroom apartment for rent in Vinhomes Gardenia
Using Area (m2)
$800/ month
Nam Từ Liêm
Tu Liem is a suburban district of Hanoi City.
There are 16 administrative units in Tu Liem district including:
· Cau Dien Town
· Communes: My Dinh, Phu Dien, Thuong Cat, Thuy Phuong, Co Nhue, Me Tri, Xuan Phuong, Dong Ngac, Tay Tuu, Minh Khai, Lien Mac, Xuan Dinh, Trung Van, Tay Mo, Dai Mo.
Headquarter of District People's Committee: Cau Dien Town - Tu Liem, Hanoi, Vietnam.
Telephone: 84.4.8372950
Tu Liem is the most populous district in Hanoi district with a total area of 75.32 km2 and a population of more than 500,000 people. Therefore, there is a plan to split Tu Liem district, the highest urbanization rate in Hanoi, into two districts.
Tu Liem district is bordered by Hoai Duc and Dan Phuong districts to the West, Cau Giay, Tay Ho, Thanh Xuan districts to the East, Ha Dong district to the South and Dong Anh district to the North.
The district is the home to many universities, colleges and new urban areas which have been formed for many years.
Tu Liem district has some famous fruits such as grapefruits in Phu Dien commune and sapodillas in Xuan Dinh commune. Tay Tuu commune is also famous flower village in Hanoi city.
Many urban areas are located in Tu Liem district such as The Manor, My Dinh Song Da building, Dolphin Plaza, etc. Among them, Keangnam Hanoi Landmark Tower is the tallest building all over Vietnam.
Some interesting and famous places are also located here such as Hanoi Museum, My Dinh Stadium, etc.
In Tu Liem district, it is so easy for you to go to the industrial parks such as Thang Long, Noi Bai – Soc Son, Thach That, Que Vo and Quang Minh.


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Samantha (Jacqueline Kim), the eldest daughter, is a tough New York businesswoman engaged to a prominent young man (Jayce Bartok). Festering beneath her controlled surface, however, is a deep-rooted resentment for being pushed onto the straight and narrow path. As she nears her thirtieth birthday, Samantha begins to reevaluate her career and love life. When she runs into an old high school flame (Rossif Sutherland) during a visit to her hometown, Samantha is thrust into a soul-searching journey that compels her to reexamine how she has lived her life until now.
Julie (Elaine Kao), the shy middle sister, is a fourth-year medical student whose only social outlet is her weekly ballroom dance class. Julie has always been the quiet center of the Wong family storm. However, Julie's world is turned upside down when she meets Mia Scarlett (Mia Riverton), a movie star researching her next role at the hospital, who sets Julie's heart aflame. While Julie grapples with the difficulties of dating a celebrity, she also tries to keep her own family from falling apart.
Katie (Kathy Shao-Lin Lee), the youngest sister, is a disaffected high school senior who engages in an elaborate prank war with Simon (Sebastian Stan), her longtime neighbor and nemesis. While the pranks start out innocuously, the incidents rapidly escalate to dangerous proportions until the two finally discover their own peculiar brand of emotional connection.
Before Ed disappears, he decides to re-visit his history through old VHS footage of the Wong family (the director's own home video footage). The stark contrast between the happier past and the colder reality of the present compel Ed to leave home. Yet, while the Wongs may no longer be able to verbally express their feelings, Ed and the daughters learn to communicate again through the stories and images from the past.


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Every year, as the season of Advent progresses, I find myself focused on a different aspect of the Christmas story.
Some years it's been Mary and the amazing faith it took to embrace her part in the Lord's plan.
Some years it's been Joseph and what it takes for a man to be father to the Son of God.
A couple of years ago it was the giving of gifts and the Lord's generosity.
Last year it was connecting the First Coming to the Second.
This year, I think the focus might be on the hiddenness of it all. How silently, how silently the wondrous gift is given.
Anyone who's been around the process of giving birth ought to be aware that this is a bit of a conceit, rather like "little Lord Jesus, no crying He makes". Jesus was, in fact, a real human being, a Baby for all intents and purposes just like every other baby, with real tears and real wails of distress. And He didn't come into the world without pain, either.
But that's not really the point. The point is that the birth of the King-Messiah wasn't in an imperial palace and heralded with trumpets.
The world, as it is wont to do, was focused on the lives and times of the rich and great. Augustus Caesar was on the throne in Rome; the Roman puppet Herod the Great ruled in the province of Judea. His building projects expanding Temple Mount and building the Herodion palace were fairly recently completed, monuments to himself and to human ideas of greatness.
And in a small village in the very shadow of Herod's fortress palace, a couple of poor teenagers displaced by the great Caesar's tax census laid their newborn in a feeding trough to get him off the floor of the barn.
The Son of God, the promised Deliverer and King, possessing more intrinsic greatness than every ruler or potentate that history has ever called "the Great", born into the equivalent of a refugee camp for displaced persons in a conquered province, to a couple of teenagers from the very bottom of the economic ladder.
In the shadow of "Make America Great Again", it's… challenging.
Jesus' homeland had no military power. It was occupied territory, under the sandaled heel of the empire that invented crucifixion as a means of execution and which came up with the terrifyingly simple Pax Romana: "Do not fight, or we will kill you". The Romans were good at killing people in job lots.
And in this conquered territory, Jesus was born in a small village. Today we tend to exalt country life as a lifestyle to strive for, but back then it was the cities that were the places everyone wanted to live; they were the safe places where you could live out your life without so much fear of bandits or thieves. In terms of how we think about different types of places, Jesus was born in an urban ghetto.
Not only that, but He was born not to rich, powerful people but to the poorest of the poor. The "pair of doves or two pigeons" sacrifice for a firstborn that Joseph and Mary made to fulfill God's Law was the very least sacrifice for the very lowest income bracket. Today, Mary and Joseph might not be earning enough to even pay income tax; back then, they were being shunted around like pawns on a chessboard by those who demanded their taxes.
Herod's greatest self-named monument to his own glory, the palace at Herodion, was visible from Bethlehem, but what a difference! Swimming pools and gardens in the rocky Judean wilderness, all constructed on a mountain effectively built by Herod's engineers, Jesus' human family would probably have looked too scruffy even to live in the servants' quarters.
Born in a stable, because there was no room in the inn. And you'd only be staying in the inn to begin with if you had no family in the area to stay with. Both of them being "from the house and line of David", where were their relatives? It seems Joseph's decision to obey the Lord and marry Mary anyway may have caused his relatives to disown them.
And so comes the King of the Universe. So very silently that even the Magi almost missed it. Not in a palace, not with trumpets. His birth proclaimed to shepherds – a profession so unskilled that it was frequently left to dozy children, of so little status that even farmers looked down on them. These are the fast-food restaurant workers and tollbooth attendants of the ancient world.
It's appropriate. The Kingdom of the Messiah is fundamentally inverted compared to what humans value. "Woe to you who are rich, for you have already received your comfort. Woe to you who are well fed now, for you will go hungry. Woe to you who laugh now, for you will mourn…" in Luke's version of the Beatitudes. Paupers, widows, tax-men, revolutionary terrorists, women and children exalted, the devout, God-fearing good folk of the Pharisee movement castigated and insulted. By the time of the early church, towns were screaming in panic that "the people who have turned the whole world upside-down have now come here!"
A Kingdom for the weak, the disadvantaged, the poor, the marginalised. Losers, misfits, the ugly and the unsuccessful, those who couldn't make a go of it in the Roman world's system. Led by a homeless man who had a political revolutionary among His inner circle and whose followers would institute a communistic economy among themselves, based on giving and sharing rather than buying and selling. The Kingdom of God is at hand.
Tremble, o world.


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Christian Academy Created for Girls in Orange Park, Florida with Behavioral, Emotional or Academic Troubles
Parents, as you search for a Christian boarding school for girls in Orange Park, Florida, realize that we are praying that you will consider Lakeland Girls Academy. While our academy is not in Orange Park, Florida, we invite you to look into what LGA has to offer.
Located in Lakeland, Florida, just an hour southwest of Orlando, LGA is within reach from Orange Park, Florida and from anywhere in the country. Teens come to our school from Florida, even though they may be looking for Christian boarding schools in Orange Park, Florida.
Gain Early Intervention, Before Your Girl Gets into More Trouble in Orange Park, Florida
LGA is a Christian boarding school for girls ages 13 to 17 and part of the well-known Teen Challenge program for struggling teens. Teen Challenge has been an effective boarding school for teens for the past 60 years.
A Christian School for Girls that Lovingly Turns Around Bad Behavior and Improves Academics for Girls from Orange Park, Florida
This Christian boarding school engages the girls in individual and group counseling sessions each week. Girls in our boarding school get life counseling from mentors and staff in the course of activities and daily life. We also include parents in periodic Family Weekend sessions to work toward healing for the entire family.
LGA offers a safe place for girls to grow and mature. For many troubled girls, academics gets pushed aside because of the other concerns they are dealing with.
While we concentrate our attention on emotional growth and healing, we also provide a quality accredited education. We emphasize individual learning. Once the girls have individually mastered one concept the next can be started without having to wait, so students are able to either catch up or get far ahead in their studies. Teachers are accessible to parents for updates on their daughter's academic progress.
Activities Here are for Therapy As Well
We acknowledge that sending your daughter to a Christian boarding school in Orange Park, Florida or anywhere else for assistance is a tough decision, but one that can bring unity to your family. Please visit the rest of our website and contact us to learn how we can help. Teens at Lakeland Girls participate in a wide variety of activities, all of which are designed for growth.
A structured schedule and daily chores develop discipline and responsibility, while intramural sports and outdoor recreation keep teens active and build positive character traits. Girls become well-rounded young women as they engage in activities from dance to cooking to community service and cultural events.
|More about Christian boarding schools for girls near Orange Park, Florida:
Orange Park is a town in Clay County, Florida, USA, and a suburb of Jacksonville. The population was 8,412 at the 2010 census. The name "Orange Park" is additionally applied to a wider area of northern Clay County outside the town limits, covering such communities as Fleming Island, Lakeside, and Bellair-Meadowbrook Terrace, making it one of Jacksonville's most populous suburbs.
Excerpt about Christian boarding schools for girls near Orange Park, Florida, used with permission from Wikipedia.
Lakeland Girls Academy is a Christian boarding school offering hope to the parents of troubled girls from Orange Park, Florida, even though it is located in Lakeland, Florida.


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Building a Diverse and Inclusive Tera . . .
A Special Message of Appreciation
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It's a small world after all
The most visionary CFOs and their finance teams have realized the benefits and importance of utilizing all of the data they generate and gather along the value chain.
Michael Ingemann is the Practice Partner for Financial Management at Teradata International. Michael started out as an auditor in Coopers & Lybrand (now PwC) in the early '90s and has ever since worked in a variety of senior finance positions in different industries (Oil & Gas and Airline). Since 2002, he has been working as a senior management consultant delivering all kinds of finance transformation projects for companies in Europe and Asia, where optimization of the Finance department and enhancing their analytical capabilities, via usage of all data, have been the key elements. At Copenhagen Business School, Michael has earned an HD in management/financial accounting (HD(R)). In addition, also from Copenhagen Business School, he has also earned an Executive MBA in General Management.


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Best Sound Award
A category that we have been promoting since we began, it seems others are jumping on the bandwagon and talking about the importance of sound in mobile games. We are seeing better and better sound and people are no longer caring about others hearing (or at least they are using headphones now). The developers are slowly starting to take notice apart from Rovio games who have always had great sound, hence they make up a lot of our finalists. Our winner may surprise you though!
And the Winner...
Best Sound


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Jackie started roasting coffee in her boyfriend's garage while attending school at Colorado State University. Jackie's love of coffee and desire from a young age to open a cafe is what caused her to empty her savings account and purchase the commercial coffee roaster when she was just 20 years old.
Jackie's plan was to spend a year teaching herself the craft, and then by the time she graduated from CSU she would be ready to open a cafe that featured a roaster right there inside the shop. Jackie admits however, that roasting coffee was MUCH more difficult then she had envisioned. It ended up taking her a year just to get the coffee drinkable!
After a year of practicing and raking up credit card debt, she was finally able to make drinkable (and delicious!) coffee and took it to the local Fort Collins farmers market. Unknowingly, Jackie sold coffee that very first week to the owner of a local coffee shop- Cafe Ardour. The second week at the farmers market that owner returned and said "That was the best coffee I've ever tasted. Can you supply our cafe?" It was an unexpected twist to Jackie's plan, but she soon became the exclusive roaster for Cafe Ardour.
Word quickly spread and without ever paying to advertise (she wasn't even listed in the phone book!) Jackie had a full-time coffee roasting business on her hands. She laughs now thinking about how hard she worked that year- finishing up her college degree on time and starting a business. But she will be quick to tell you that she loves what she does and can't imagine her life any other way.
Today, Jackie owns and operates Jackie's Java out of a warehouse in Fort Collins. As the owner, she does a little of everything around the warehouse! If you stop in, you may see her working in the office or hear her on the phone with a customer. She also continues to roast a lot of the coffee, still mans a few of the farmers market booths, and does some deliveries when she can.
Her favorite part of the job is the sales. She says she never feels like she's pushing anything on anyone, people want Jackie's Java and she's happy to supply it! Jackie says she loves the industry and meeting new coffee enthusiasts like herself. She belongs to a "Coffee Cult" – a club of roasters, exporters, farmers, and importers who work together to try and further the industry. Jackie also continues to travel to various coffee origins seeking out new small farmers to purchase from as well as exciting new coffees to import.
Jackie says her favorite coffee to drink varies a lot, but right now she's a fan of the light roasts. They are refreshing instead of sour, which is something unique about her coffee and the way she makes it.
In the rare moments when she isn't at work, Jackie likes to play with her dog and take him to the dog park, and in the winter she also loves to go snowboarding.
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Thomas Cardinal Collins
As a champion of the full spectrum of life from conception to natural death, Cardinal Collins adamantly defends life in a world where many governments actively counter all pro-life services to the developing world. His Eminence encourages Lectio Divina (personal encounter with God in Sacred Scripture) and promotes a deeper love of the Faith in the Archdiocese of Toronto.
Bishop Robert Barron
Perhaps one of the best-known Catholic leaders in the U.S. today, Bishop Barron serves as Auxiliary Bishop of the Archdioceses of Los Angeles. Masterfully utilizing media, the founder of Word on Fire Catholic Ministries, host of the Catholicism series, #1 Amazon bestselling author, television, YouTube (viewed over 25 million times) and Facebook personality brings the Faith to millions.
Father Leon Pereira, OP
Raised in Singapore, Dominican Order Father Leon, OP is a gifted homilist who brings humor, unabashed forthrightness and humility to the pulpit. Previously serving in England and now in Medjugorje, Bosnia-Herzegovina, Father Leon shares his love of our Holy Mother and the wisdom he has gained from experiencing a conversation with her. He also offers insight into the actions that can get us to heaven.
Steve Ray
Known as the "Indiana Jones" of pilgrimages, Steve and his wife Janet have guided over 100 Holy Land tours. The depth of truth in the Catholic faith led Steve to leave his Baptist roots and convert to Catholicism in 1994. Since then, his consuming devotion to the Faith has inspired him to write, speak on Catholic TV, radio and at conferences, produce films and lead thousands to Biblical sites.
Renée Bondi
A beloved speaker at the 2010 Christ Our Life Conference, Renée offers encouragement to all. Suffering a bizarre accident which left her quadriplegic at age 29, she also lost her exquisite singing voice. Through boundless perseverance, Renée's voice was miraculously restored. She shares the lessons of faith that Jesus taught her in her darkest days and the messages of hope she found in surrender.
Matt Fradd
As a Catholic speaker, apologist, author and podcaster, Matt informs us on a wide array of significant topics from God and the universe to pornography addiction and chastity. He also serves as director of content development for Integrity Restored and directs The Chastity Project. Matt assists those trying to break free from the addiction of porn by utilizing the tools of education, example and humor.
Magnus MacFarlane-Barrow
The Christ Our Life Conference is again privileged to welcome Magnus from Scotland, home to his organization Mary's Meals. This humanitarian's love for Jesus and His Blessed Mother Mary, motivates Magnus to serve the least among us, working through Mary's Meals to provide a daily school meal to over 1,230,000 of the world's poorest children while continuing to work to feed countless others waiting to receive daily nourishment.
Jimmy Belabre
Described as one of the most dangerous slums in the Northern Hemisphere, Cité Soleil, Haiti is home to musician Jimmy. Subsisting on nourishment from Mary's Meals each day at school throughout his young life, he refused to accept money and guns from local armed gangs and instead resolved to remain in school. Now the principal of the Cité Soleil school he attended, Jimmy provides an example to all.
Steve Angrisano
Musician, composer and storyteller Steve delights audiences with his talent, sensitivity and devotion to Christ. Providing the non-liturgical music for the 2018 conference, his performances demonstrate his aptitude for timely story weaving. Steve has performed at six World Youth Days, numerous National Catholic Youth Conferences and events worldwide, always sharing his authentic and deep faith with his devoted audiences.
Lupita Barajas
Recognized as an international speaker who guides her audiences about topics including family spirituality and formation, Lupita hosts two radio programs on En Familia Radio in Phoenix, AZ and is a frequent guest on TV and radio. A family counselor, she also serves as a consultant to parents and is a facilitator at a the School for Parents in several schools throughout Mexico.


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The top picture is my granddaughter looking at a slot book that we gave our son (her Daddy) for his 2nd birthday!! How fun is that? She loves this book, and so did her Daddy.
Sawyer is just hanging out being cute...and drooling like crazy.
The paper is KC & Company and most of the embellishments are from Glitz Design. The journaling card is Doodlebug Design. I love the alphabet paper. It was a scrap that I had in my stash. It's fun using my scraps whenever possible, but I use them for card making than scrapbooking.
Thanks for stopping by my blog today!!