text:
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Contact a sales agent to assist you with your purchase.
The Solare 3D Virtual Flame Candle is the world's first
electronic candle that looks, acts and moves like a real candle.
Each Solare uses proprietary technology to create a three
dimensional flame that is visible from any vantage point and
indistinguishable from a real flame. The Solare's virtual flame
emits light and moves randomly, just like real fire. Each Elite
Solare makes a captivating stand alone accent in any decor.
Creating the illusion of a real candle light without the mess and
danger of fire, the Solare will create a distinctive atmosphere in
any setting.
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Real Salt Lake Women make their final road trip of the season this coming Saturday, June 25th, with a visit to Santa Clarita for the return match against the Blue Heat.
Only three games remain in the inaugural UWS season for RSL, and the team is in a great position with 10 points from a 3-1-1 record that has them in first place in the West Conference. They are closely followed in the standings by Houston Aces, whose 3-3-0 record has them at 9 points with only two matches remaining in their season.
RSL has a few things in their favor to give them the chance to finish strong and claim a spot in the first ever UWS Championship match. Despite playing to a draw at home against Santa Clarita in their first meeting, thanks to late heroics from Bizzy Phillips' converted penalty shot, RSL played a strong match and were close on several good chances that could have secured a victory.
Their recent run of form should give them a lot of confidence in executing their style of play. RSL has been especially solid on the road, having wins in all three matches played away from Utah so far, and are facing a Santa Clarita team that lost their previous home match by a 4-3 score against Houston. They have a great opportunity to come out aggressive and finish with a perfect road record against the Blue Heat, who have just one win in 4 matches played, that being the unusual 10-1 season-opening victory in Houston.
The team will still be missing co-captain Annie Hawkins, who still has not been cleared to play following the concussion she sustained against Santa Clarita on June 11. RSL has seen a lot of strong performances in her absence, especially the way that Kira Sharp has taken over Hawkins' position in the midfield, with impressive play on both sides of the ball in Colorado last week.
The team will continue to rely on the full 18 players in the matchday roster to use their speed and high energy to put pressure on the hosts and finish the season with a perfect road record. The players' dedication and persistence has grown with each game. The team is getting important contributions from everyone in the lineup, and are being rewarded with positive results for their complete team efforts.
Kickoff between RSL and Santa Clarita is scheduled for 9:00 EDT/6:00 PDT.

subset: commoncrawl


text:
Isadora's currently has some of the most exquisitely shaped Perfume Bottle's. And given the inherent feature of perfume, from the conviction it gives its wearer to the indefinable impact it sometimes has on those around the wearer, it's not surprising that perfume has long been kept in these mystically shaped bottles whose shapes seem to echo the mysterious properties of the fluids inside them. Whether it's a surprisingly detailed hammered pocket size, slender Art Deco crystal, a robust Czechoslovakian bottle, a tiny lachrymatory, or a fabulously deck-out with paste stones, perfume bottles are designed to contain magic, which is only unleashed when the bottle is opened and a drop or two of the precious liquid is discreetly applied.
These alluring bottles reminded so much of a fantastic novel by Tom Robbins entitled, "Jitterbug Perfume." The major themes of the book include the striving for immortality, the meaning behind the sense of smell, individual expression, self-reliance, sex, love, and religion. Beets and the god Pan figure prominently. The novel is a self-described epic, with four distinct storylines, one set in 8th century Bohemia and three others in modern day New Orleans, Seattle, and Paris. If you haven't read this book I highly suggest it. I imagine the epic perfume described in this novel to be encased in an equally epic bottle.


text:
What tools are enterprises using for project management? And what are the channel opportunities?"Email is dead. If you have tasks in email, will they get done? No."
This, according to Karl Fischer, a senior technical consultant at Johannesburg open source services company Obsidian Systems, is why organisations are increasingly using the Atlassian toolset.
Atlassian is a Sydney-based company which makes enterprise collaboration software. It posted revenue of $457-million in 2016, and acquired project management service Trello earlier this year for $425-million. Other products in its toolset include the project and issue tracking software Jira, Confluence for document collaboration, and HipChat for screensharing and team chats.
At the moment, Obsidian is one of three Atlassian partners in South Africa. It has a gold partner status, while the others have been rated as silver. Obsidian held an awareness event in Johannesburg this week, which was attended by mainly financial services institutions.
There is however a recertification process underway, Obsidian managing director Van Staden told the Margin.
"We need four certified technical people and four certified sales people to stay on gold, and for platinum it's eight."
Partners install the software and then provide training at a company or enterprise.
"We'll understand their business processes and consult on how to use the toolset to achieve their business goals," he says.
The product is priced on a per-user model in the cloud and when it's installed on a company's server. Enterprises would sign an enterprise level agreement and pay a set fee for the full tool set of Atlassian technologies.
This doesn't lock out smaller players however, and most of the Atlassian starter packs can be bought for $10, certainly in the reach of any SME.
Something for everyone
Van Staden says Atlassian also donates all the money from the $10 sales to charity, which adds up to hundreds of thousands of dollars every year.
"It really caters for everyone. If you're an academic, you get academic pricing. If you're an NGO, you get all of their stuff for free. They really try to help organisations achieve their potential."
"With Atlassian, it's crazy to think that it doesn't have salespeople. Its whole strategy is that they want to build software that is so good that people want to use it. And that's how their whole company has been built."
Obsidian's Fischer says one reason enterprises were choosing Atlassian over other programs such as Slack, was because the tools could be installed on its internal servers, and information would not leave the company's network.
"People in organisations want to talk to one another instantly. They're looking for agile and quick ways to do a turnaround of their projects."
What are the opportunities for the channel?
Van Staden says there's a huge consulting need around this technology, and there's lots of scope for people to get involved and provide services, or retail, around the product set.


text:
Beach Cottages 30 meters from Polzeath beach in Cornwall
Lovely Beach Cottage only 30 meters level walk to the beach - Sleeps 4
Waves End is situated about 30 meters from the beach and offers 1 double room and 1 twin room sleeping up to 4. Open plan living/dining/kitchen with 1 bathroom. Outside seating area.
Waves End is a Luxury 4 Star Gold Beach Cottage only 30 meters level walk to Polzeath beach, restaurants, cafes and shops.
Polzeath Beach is a stunning sandy beach with surf schools, cafes and shops and ideal for all ages.
Prices include bed linen, bath towels and Free Wifi.
Bedroom 1 has a luxury 2000 pocket sprung kingsize bed. Bedroom 2 has two single beds with luxury pocket sprung matresses.
Sitting Room has two comfortable sofas to seat 4 persons and 42" Wifi TV
Dining Area has table and chairs for 4 persons
Kitchen is well equipped with cooker, hob, microwave, toaster,kettle,fridge,dishwasher & washing machine.
Outside is a sunny seating area that gets the sun all day and late into the evening.
A 15 minute stroll up the lane to a country club with golf course, driving range, indoor pool, jacuzzi, steam room, sauna, gym, tennis courts and beauty treatments.
Activities include surfing, sailing, wind surfing, water-skiing, stunning coastal walks. Take the ferry from Rock to Padstow and visit Rick Stein's restaurants.
Sorry no under 25 year olds without parents unless agreed with owner firstNo single sex young groups unless agreed with owner firstRefundable security deposit £100. Situated next door to Tides Reach so can therefore be booked together to sleep 8.
VisitBritain 4 Star Gold property at last assessment.
Sorry no pets accepted.
This property has been listed with Holidaybank for 11 years
Use the zoom controls to view the map in more detail. You can also drag the map by clicking and moving your mouse at the same time.
Please phone Mr Dominic Leese
(+44) 01208 869576 or (+44) 07717 222574
Don't forget to mention Holidaybank when you call.
Don't miss out on regular holiday offers, accommodation news and update emails from Holidaybank, Chez Nous, Stilwell's Cottages Direct and Cottages Online – just enter your email address below:
Contact Holidaybank | Privacy Policy | Cookie Policy | Terms of Use | About Us
| Modern Slavery Statement
Holidaybank is a trading name of Vacation Rentals (UK) Ltd. Registered in England and Wales. Company Registration Number 00965389.
VAT Registration Number: GB 598 22 99 77
Waves End in Cornwall. Enjoy holidays in Cornwall


text:
Here's a closer look at the heart balloons and the semi transparent layers. I just love the tassels in this set that really sets this stamp set apart. It's perfect with balloons of all shapes and great for celebrating any festive event!
And because I'm so psyched about the Party Kit stamp set, I created another card for a special birthday boy who celebrated his second birthday just this past weekend. This time, I used the round balloons and added complimentary green tassels in varying tones as well as the sentiments as the balloon strings. So fun and so versatile -- And that's why you'll want to get in on the Mama Elephant promotion!
Materials used: Mama Elephant Party Kit, Heidi Swapp Serendipity pattern paper, sequins
Thanks for stopping by!
- KRAFTY pearl


text:
Although globalization has greatly benefited many people around the world, it hurt a lot one particular class of people.
The "elephant" chart below shows who benefits and who is hurt by globalization.
Globalization greatly increased income of:
If Facebook, Twitter, WhatsApp, and other social media were countries, this is how they would compare to "real" countries.
Women tend to live a little longer than men.
But in some countries, women live MUCH longer than men.
Here are 10 where the gap in male vs. female life expectancy is the biggest.
Global trade today is not as it was 25 years ago.
There is a nearly perfect negative correlation between wealth and working hours: the wealthier the country, the less its people work.
There is, however, one notable exception: the U.S. It is very, very rich. And Americans work very, very long hours.
As the new generation enters the labor force, companies have to adapt to the values of the millennials.
Unlike their parents who were happy with secure 8 to 5 jobs, the millennials are driven by other ideals and desires.
These are the biggest changes in Top 3 motivators at work for those born in the 60's and 70's vs. those born in the 90's and 00's.
Here is a map that allows you to put it in a perspective.
– The GDP of Saudi Arabia with its massive oil production is only as big as the GDP of Illinois.
– Switzerland's economy, with all its banks, cheese, chocolate and army knifes is only as big as that of Pennsylvania.
– Or the GPD of the Netherlands is only as big as that of Florida.
International business vocabulary: Currency Buy-Sell Spread
What the U.S. thinks about Europe?
What Europeans think about Americans?
What northern Europeans think about southern Europeans?
What southern Europeans think about northern Europeans?
X-culture.org ®. All rights reserved.
Questions, suggestions: Dr. Vas Taras, email@example.com or 1(336)256-8611


text:
Senior Associate , Banking Intelligence
- Job Description and Requirements
This Role for taking the lead in technical architecture, to design and build out the data engineering platform that will form the nucleus of the Analytics, BI and Machine Learning initiatives for Banking Business. The role will involve building and maintaining database solutions that are used for daily reporting and drive business decision making while dealing efficiently with the massive scale of data available through Data Warehouse. Also responsible for designing and implementing solutions that involve data interfaces with both internal and external systems using third-party and in-house reporting tools, modeling metadata, building reports and dashboards, and administering the platform software. Responsible for developing , maintaining , testing and evaluating big data solutions within the Bank.
Job Resposnsibilities:
- Work closely with the analysts and business stakeholders to gather technical requirements.
- Provide data insights for product management and marketing decision making.
- Participate in "deep-dive" research projects, such as detailed product analysis, customer segmentation or lifetime value analysis.
- Build new data views, aggregations, dashboards and predictive models.
- Contributing at a senior-level to the data warehouse design and data preparation by implementing a solid, robust, extensible design that supports key business flows.
- Performing all of the necessary data transformations to populate data into a warehouse table structure that is optimized for reporting.
- Establishing efficient design and programming patterns for engineers as well as for non-technical peoples.
- Designing, integrating and documenting technical components for seamless data extraction and analysis on big data platform.
- Ensuring best practices that can be adopted in Big Data stack and share across teams and BUs.
- Providing operational excellence through root cause analysis and continuous improvement for Big Data technologies and processes and contributes back to open source community.
- Contributing to innovations and data insights.
- Build new data views, aggregations, dashboards and predictive models.


text:
Crispy French Fries topped with lightly breaded cheese curds and drizzled with rich gravy.
Warm and creamy dip made with tasty crab blend, cream cheese and Cajun seasonings.
Cream cheese, duck, bacon and spices stuffed in a wonton wrapper.
Sauteed mushrooms served with hickory-encrusted steak tips.
Grilled Romaine with carved tenderloin medallions, bacon, roasted tomatoes and onions, drizzled with parmesan peppercorn and balsamic glaze.
From the Water
Served with your choice of soup or salad, and rolls.
Also accompanied by your choice of potato or rice.
Substitute a caesar salad for 1.99. Add bleu cheese for 1.99
Sauteed shrimp in a garlic herb cream sauce, accompanied with heirloom tomatoes and a pasta nest.
Seared Ahi tuna prepared medium rare, served over sauteed vegetables with an Asian dipping sauce.
Harvested in cold water and sustainable raised. Simply the best.
Try it blackened - 21.99
Large 8 oz. Caribbean lobster tail, not just for special occasions!
A tradition as old as the lakes!
Jumbo shrimp, prepared your choice of lightly breaded, broiled or stuffed.
Served with your choice of soup or salad, and rolls.
Also accompanied by your choice of potato or rice.
Add sauteed mushrooms for 4.99, bleu cheese for 1.99, garlic-mushroom-cognac sauce for 1.99, or blacken any steak for 1.99
12 oz, well-marbled and delicious.
8 oz center cut sirloin.
Tender medallions, sauteed in garlic, onions and mushrooms.
Have it garlic stuffed or bleu cheese stuffed for 1.99 each
Beef medallions and Chianti beef ravioli, served with a Tuscan butter cream sauce.
Large portabella mushroom served with red quinoa, fire roasted vegetables, rice and mozzarell. Topped with parmesan and baked over creamy marinara. Delicious!
Slowly-roasted baby back ribs with our homemade sweet and tangy barbeque sauce.
Full Rack 27.99
Half 20.99
Quarter chicken paired with a half rack of ribs.
Sundried tomato and garlic-encrusted pork ribeye.
(Saturday - While It Lasts)
Jack 24.99
Queen 26.99
King 28.99
Citrus marinated chicken breast, grilled over quinoa rice blend, topped with pineapple salsa
Chicken breast and mushrooms sauteed in creamy Marsala wine and served over pasta
Lightly breaded chicken breast served over a bed of rice, drizzled with Hollandaise sauce
Half a chicken. All white meat $3 extra
Sauteed chicken breast topped with marinara and mozzarella cheese, served on a bed of pasta
Served with regular cut fries and coleslaw.
Add cheese for .50 extra
Fish Fry - $12.99
Bluegill - $15.99
Lake Perch - $15.99
Baby Walleye - $15.99
Shrimp Basket - $13.99
Prime Rib
King - $27.99
Queen - $25.99
Jack - $23.99
Stuffed Shrimp - $18.99
Pot Roast w/ Mashed Potatoes, Gravy and Vegetable - $13.99
Coconut Shrimp -$16.99
Chef's Special


text: posted 03 may 2019 location chengdu shi, international specialism international teaching sub specialism Secondary job type contract working hours Full-Time experience Teaching reference number BBBH23161 contact the far east team, teachanywhere apply now apply now Email this job to yourself or a friend job description Secondary ICT Teacher Start Date: August 2019 Location: Chengdu, China Region: Far East and South East Asia Curriculum: UK curriculum Experience: 2+ years Salary: from 17,000 RMB to 25,000 RMB after tax per month Sponsorship: Single or Married teachers with children Contract: 2 yearsSchool overview: This boarding school opened in September 2015 and is very closely affiliated to a highly prestigious UK boarding school, founded in 1865 with an excellent reputation. The school is rapidly expanding with a full potential capacity for 2000 students from KG - 18 years. A British based curriculum allows students to study IGCSEs and A-levels with many students working towards applications for overseas university careers. The school strives to be the leading boarding and day school of the region, and seeks to develop happy, balanced individuals capable of achieving personal fulfilment at university, at work and in society at large. Whilst a strong focus is on academics, the broad curriculum is complemented by an extensive sporting, musical, artistic and drama programme. Together with a strong emphasis on pastoral care, they believe these co-curricular activities give pupils the opportunity to maximise their potential.The school is situated next to the Botanic Gardens in the North of Chengdu, with a campus area of 100,000 sqm and 65% green coverage. Teaching buildings are specially designed for interactive learning and feature multi-media classrooms, language and science laboratories, all equipped with the latest educational technology. The facilities in the campus include an outdoor amphitheatre, a café, mini-golf course and basketball courts providing a beautiful learning environment for pupils.Location Overview: Come face to face to with the enigmatic and critically endangered giant panda in Southwest China's hub, Chengdu. The city is famous for its expansive panda breeding centre where visitors can get up close and personal with these symbolic creatures. If cute and cuddly bears are not enough of a draw, the region of Sichuan is home to its very own distinctive and delicious cuisine, filled with bold flavours thanks to an abundance of chillies and peppercorns. Chengdu has a reputation for possessing a care-free lifestyle, and many find the city hard to leave. Teahouse culture is central to Chengdu, and there are more teahouses and bars to be found here than in Shanghai, despite having less than half of the population. On top of all this, you can take the opportunity to play Mahjong and really become one of the locals, as the game is played almost everywhere by all walks of life.Requirements: * Applicants must have 2+ years teaching experience. * UK curriculum experience is highly desirable but not essential. * As with any new and expanding school, you should be a teacher who is prepared to be flexible and adaptable and open to the developing nature of a new school environment and possesses the resilience this can require. * You should be sensitive to cultural differences and work well under pressure. * A willingness to contribute to all areas of school life is essential - given the boarding nature of the environment. * Previous international teaching experience is desirable.Package: * Salary from 17,000 RMB to 25,000 RMB after tax per month * On site accommodation is provided or an attractive allowance if off site preferred. * Annual flights * Medical cover * Relocation allowance * Tuition for up to 2 dependents. skills knowledge of curriculum


text:
Shakespearean Stars
Research one of the many actors who have become famous because of their performances of Shakespeare, such as Richard Burbage, Edwin Booth, John Barrymore, Edith Evans, or Peggy Ashcroft. Make sure you choose someone with a substantial background in Shakespeare (e.g., not Leonardo DiCaprio because he did Romeo and Juliet once). Create a poster that includes the following:
-- actor's name
-- actor's year of birth and death (if appropriate)
-- what Shakespeare plays or films they did
-- which role, if any, they were especially famous for
-- a photograph or drawing of the actor
We will use the posters to decorate the classroom.
King Gertrude? Princess Hamlet?
Did you know that when Shakespeare's plays were first performed all the parts were played by men? Even the female characters were played by young boys whose voices were high enough to sound like girls. So...
This section contains 1,504 words
(approx. 6 pages at 300 words per page)


text: |The hiring for these positions is postponed until Fall 2018. However, all applicants who remain in the list will be considered in the new searches. The Mathematics Department at The University of Tennessee, Knoxville seeks exceptional candidates to fill anticipated tenure-track, assistant professor positions that are part of a cluster hire related to analysis of data. A PhD or equivalent degree in mathematics or mathematical sciences, including statistics and mathematical biology, is required. Some postdoctoral experience at a major research university is preferred. The Mathematics Department is currently writing a proposal for a cluster hire involving four assistant professors and two higher-rank faculty. More details may be found in our advertisement for higher-rank faculty connected with the cluster. Applicants for the assistant professor positions are encouraged to bring that advertisement to the attention of their dissertation or postdoctoral advisors, or other prominent researchers with whom they would be interested in working as part of a cluster at the University of Tennessee. Evidence of potential for excellence in research and high quality teaching is required. Review of applications will begin if and when the department's cluster hire proposal is accepted, likely in early to mid-January. This advertisement will be updated to reflect any changes in the timeline. The University of Tennessee encourages applications from candidates who have the ability to contribute in meaningful ways to the diversity and intercultural goals of the University. The University of Tennessee at Knoxville is the flagship university in the Tennessee state higher education system. In recent years, the Department of Mathematics has increased its research prominence both within the University and in the larger mathematical community. Opportunities exist to collaborate with researchers at Oak Ridge National Laboratory (ORNL) and the National Institute for Mathematical and Biological Synthesis (NIMBioS). The department is highly supportive of its faculty, including providing full travel support for 99% of invited talks by departmental faculty in the last five years; summer research stipends and travel support for advanced PhD students; partial F&A return from grants; office space in beautifully renovated Ayres Hall, the iconic building of the University of Tennessee; competitive salaries and teaching loads; and high quality administrative support. The University of Tennessee offers a very competitive benefits package. Knoxville is a medium-sized city nestled in the Tennessee Valley, which is bordered on one side by the Great Smoky Mountains and on the other by the geologically rich Cumberland Plateau. The city has a relatively low cost of living, and there is no state income tax on earned income. The city caters to diverse interests: the downtown area has a vital Arts District, Old City, Market Square, and Waterfront, while less than three miles away across the Tennessee River is an Urban Wilderness larger than Central Park in New York City, with more than 45 miles of hiking, running, and mountain biking trails. Candidates should apply via MathJobs.org, including curriculum vitae, description of their research accomplishments and plans, teaching statement, publication list, and three or more letters of reference. The letters should include one that addresses teaching. Candidates should also provide a cover letter specific to the University of Tennessee assistant professor position, including the names of faculty at the University of Tennessee with whom the applicant would like to have research interactions and any other reasons that this position is of interest to the applicant. The University of Tennessee is an EEO/AA/Title VI/Title IX/Section 504/ADA/ADEA institution in the provision of its education and employment programs and services. All qualified applicants will receive equal consideration for employment and admission without regard to race, color, national origin, religion, sex, pregnancy, marital status, sexual orientation, gender identity, age, physical or mental disability, genetic information, veteran status, and parental status.


text:
Better Launcher provides instant access to all your applications, documents and folders. Simply press the F10 key and start typing the name of the item you are looking for. After just a few keystrokes Better Launcher will have found the right...
Indexing, Information, Launch, Launcher, Organizer, Scanning
16-October-2004Shareware(Free To Try)
A Better Finder Launcher provides instant access to all your applications, documents and folders. Simply press the Control-Command-L hot key and start typing the name of the item you are looking for. After just a few keystrokes A Better Finder...
13-September-2006Shareware(Free To Try)
True Launcher is a simple, easy to use program launcher. True Launcher allows you to combine your shortcuts into groups, helps to free the desktop from numerous icons, folders, and files by moving them to the True Launcher start panel.
Launchbar, Launcher, Quick Launch, Quicklaunch, Shortcuts, Start Menu, Toolbar, Truelauncher
Batch Launcher allows launching/autoloading several applications by one click. The applications that you wish to launch get united in a batch - just drag and drop needed shortcuts to the Applications list of the batch.
Applications, Batch, Files, Launcher, Manager, Package, Startup
09-March-2003Shareware(Free To Try)
Rapid Deployment is a simple freeware url launcher tool that allows you to create and launch a customized list of regularly visited urls in separate default browser windows with only one click. Webmasters and anyone who surfs a great number of web...
Create, Download, Explorer, Free, Freeware, Internet, Launcher, List, Netscape, Url, Windows
SD Screen Saver Launcher the program for fast start of the screensaver. SDSSL helps to save confidentiality of the information. Now you can start the screensaver during necessary time with only one movement of the mouse.
Fast, Launcher, Mouse, Saver, Screen, Sd, Sdssl, Ssl
BigFoot - ThE PaW is a basic computer control and program launcher. It sits on your desktop when the computer is turned on and it stays always on. No need to find or launch it. The basic preconfigured controls let you turn off the monitor, shut off..
Energy, Glare, Image, Launcher, Monitor, Saving, Screen, Screen Saver
09-February-2007Shareware(Free To Try)
Using launch menu you can start any program, file or folder you want - by one mouse click (just one!). Place the mouse at top-left screen corner and menu will be shown automatically. Add items you need to the menu, and start them at any time.
Launch, Launch List, Launch Menu, Launcher, Menu, Mouse Command, Mouse Launcher, Mouse Tool, Neo Dagger, Neodagger, Shortcut, Sigma, Sigma Launcher, Sigmalaunch, Sigmalauncher, Slaunch, Tool, Utility, Window Manager, Windows Tool
31-January-2007Shareware(Free To Try)
Create a native Windows EXE to launch your Java application! The native launcher finds a suitable Java™ Runtime Environment and starts your Java application using the found JRE. JEXECreator features: Java Runtime Environment auto detection,...
Auto, Autodetect, Detect, Download, Exe, Gui, J2re, Java, Java2, Jexecreator, Jre, Launch, Launcher, Native, Runtime, Win32, Windows, Wrapper
17-March-2005Shareware(Free To Try)
CSFastRunII is a desktop enhancement that allows users to run applications, groups of applications or specific data files like documents, spreedsheets, development projects, etc from an easy to understand dropdown list control housed integrated...
Application, Deskband, Deskbar, Launcher, Toolbar
27-January-2004Shareware(Free To Try)
Free Application Launcher Tool. If you need to start several programs simultaneously, this program can be useful to you.
Using Stock Startup it is extraordinary easy to create a package from programs and to start it.
Application, Download, Free, Launch, Launcher, Package, Program, Tool
dLauncher is a simple, easy to use program launcher. A good alternative to the Windows Start Menu. Friendly and intuitive. It provides quick access to your favorite programs, shortcuts, folders, documents and websites.
Dlauncher, Launchbar, Launcher, Quick Launch, Quicklaunch, Shortcuts, Start Menu, Toolbar
Quick access to your favorite applications, documents, folders and network shared.
Automatically start programs at specific times. Reminder.
Shutdown the computer at specific times.
Automatic launch of files and programs after loading system.
Launcher, Placesbar Constructor, Program Launcher, Program Starter, Quick Launch, Quick Launcher, Quick Starter, Reminder, Scheduler, Starter
31-March-2007Shareware(Free To Try)
Thunderbird-Tray is a system tray launcher tool for Mozilla Thunderbird, the popular e-mail client. You can use TB-Tray to hide Thunderbird to the tray while you don't need it. When you click the tray icon, TB-Tray will restore Thunderbird. It...
Client, E-mail, Mail, Minimize, Mozilla, Tb-tray, Tbtray, Thunderbird, Thunderbird-tray, Tray
Zero Code Designer is A true windows application design program.It requires no programming skills at all. It is so easy that even small children can use it. No Coding needed. Start the designer, add some items to the designform ,link them together...
Design, Development, Edit, Editing, Rad
MyCleanDesktop is a icon organizer / application launcher program for the windows desktop. MCD allows you to store your desktop shortcuts and files in categorized mini-desktops that are accessible with a mouse click and close automatically.
Calendar, Clock, Desktop, Icon Manager, Launcher, Mp3, Notes, Organize
30-January-2006Shareware(Free To Try)
Tray Commander is a launcher that was developed to let you quickly run your frequently used
system commands directly from the system tray. It can open\close your CD drives, reboot, shutdown
your system, run applications, and more.
Command, Control, Launcher, Launchpad, Menu, Shortcut
07-December-2005Shareware(Free To Try)
Keep changes to your desktop icon layout once and forever. This is the final answer to the Windows desktop imperfection, keeping a strong backlog of icon layouts. Comes with a desktop simulation tool, and a powerful tray application launcher.
Desktop, Extensions, Icons, Layout, Powertoys, Tools, Utilities, Windows
02-February-2007Shareware(Free To Try)
V-Scheduler is a reminder and application launcher designed with total flexibility in mind. It consists of a highly intuitive user-interface and is extremely easy to configure. There is no limit to the number of schedules that can be programmed.
Message Reminder, Program Launcher, Scheduler, V-scheduler
06-March-2005Shareware(Free To Try)
CrossFTP is a free multi-tab GUI FTP client for stable transfers. It uses an Explorer-like interface and the novice user can master in minutes. Main features: multi-tab, FTP server launcher, anti-idle, i18n support, archive browsing, etc.
Client, Ftp, Network Tool


text:
I'm an Erotica, Romance writer who's mind is hard to silence. When I'm not writing I'm reading. When I'm not reading another author's words I'm writing my own. I've released a nonfiction memoir series under the pen name JC Cerrigone. Please visit http://www.mikestreeoflife.com I live in rural Pennsylvania with an imagination that almost never rests.
www.jc-hotreads.com www.bookstrand.com/jc-szot http://www.amazon.com/JC-Szot/e/B008BTX0W2/ref=sr_tc_2_rm?qid=1347756874&sr=1-2-ent http://www.barnesandnoble.com/s/Erotica-fiction-by-JC-Szot?keyword=Erotica+fiction+by+JC+Szot&store=book http://www.facebook.com/steamread http://jcszot.blogspot.com/
For my nonfiction works under the pen name JC Cerrigone visit http://www.mikestreeoflife.com


text:
I'm so excited to be attending my first Bouchercon! This year it's at the Vinoy Renaissance Hotel in St. Petersburg, Florida. Marchand's Bar is a popular gathering place for writers, readers, reviewers, and booksellers – meet and mingle with your faves.
If you're attending, you can see me at the Cooking Up Cozies-Writing Culinary Cozies panel in Royal 3AB, at 12:00 on Friday (9/7). Please say Hi if you come – I'd love to see you. I'll have a treat, a read, and a cup of tea for everyone who comes to the panel.
Can't make it? I can't give you a virtual treat or cup of tea, but I can give you a read: go to BookHip.com/XSWRBF and you can get a free book! To introduce you to my Beach Tea Shop Mysteries, I'm giving away Dying for a Cuppa: Collected Cozy Mysteries, Volume 1.
Safe travels to all who are going to Bouchercon, and I hope to see you there.


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NoHo News: What's happening in the neighborhood... our picks. NoHo Life: fun in one square mile of Los Angeles.
We look forward to sharing lots of fun and unique news, events, profiles and updates on what's happening in the NoHo Arts District.
"Titans of Soft Rock" dock on the Sunset Strip the first Friday of each month at the world-famous Viper Room in Hollywood.
We're listening to J-Ax & Fedez "Pieno di stronzi" from the album, "Comunisti col Rolex." Ciao, 2017. You've been a year of such divisiveness and indecency, and just an unprecedented deplorable year.
The NoHo Arts District dot Com team likes to showcase the immense talent we have in our growing one-mile neighborhood. We had the pleasure of meeting and chatting with North Hollywood band Glass Battles.
Mumford & Sons are back with their new album 'Babel' and credit should be given where it is due. The record, in terms of sound quality, composition and lyrics are fantastic. Once again the band strip down music to the bare essentials and make it work for them. Songs like "Reminder" and "The Boxer" are perfect poetic stories that, whilst being short, have a huge amount of depth to them.
Moses Luster is an American musician who's debut album 'I'm The Lion' is something that is unusual to say the least.
Van Ghost is a five-piece rock band weeping with soul. Whilst they have their own individual sound, listeners might be reminded slightly of bands such as Matchbox Twenty.
The Sun Explodes is a five piece rock band that fuses several styles of rock with electronic to create their distinct sound. Their debut album 'Emergence ' is welcomed with open arms in my eyes. It is fresh and exciting in all the right places. Some might say that they have similarities with the likes of Enter Shikari, this is true and they have the potential to rival them.
I would give anything to have lived in the times of Otis Redding, James Brown, Sam Cook, Young Aretha and Tina and others of their time. I've watched past television performances and they all radiate this sweaty, passionate essence behind their wails and screams. They also really knew how to MOVE. We live in a time where all artists are little to polished. There's a lack of letting go in fear of the internet backlash that might surface post performance. Back then, Tina's hair would whip while her raspy voice belted. Aretha wouldn't let drops of sweat get in the way of her sexually driven vocals. Otis' voice would crack and sweat stains would penetrate his three-piece suit…and it was brilliant.
Do you have an event, video or news to share? Drop us an email and you may see it on NoHoArtsDistrict.com
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About Fiona
Hi there!
This site is now mainly used to platform my work as a children's food writer and consultant. I've more recently been working on an updated, slightly more grown-up website, showing off my new married name - and covering broader aspects of the work I do - from PR consultancy to my latest project: @HomeCastleCary
Great to see you here. But click below - and I'll see you after the jump. Fiona x
"The Harry Potter of Vegetables!"
ITV Lorraine
Fiona is a self-taught and highly creative home cook as well as author, food journalist, food stylist, menu planner and consultant.
She's also a travel writer, blogger and broadcaster with a background in live radio and voice-over.
Fiona has a niche interest and expertise in children's eating habits and as such is the founder of the highly acclaimed Toddler Chef - a business inspired by her own experience of motherhood.
Her first cook book 25 Foods Kids Hate and How To Get Them Eating 24 (New Holland) has now sold out its entire first edition. It featured widely in both national press and key digital media - including The Sunday Times, The Telegraph, Red magazine and Netmums (with whom Fiona now frequently collaborates - offering ideas on creative baking as well as the healthy stuff!
Fiona's innovative and informed slant on healthy eating has seen her frequently pop up on our TV screens from ITV to Sky News (see mini film below). Her own blog demonstrates a thoughtful approach to the usual rhetoric.
She's frequently asked to develop recipes for corporate clients ranging from Morrisons to Unilever, filming online content with her signature friendly and approachable style. She's been invited to design new dishes and menus for the travel and leisure industry with past projects including an innovative new children's menu for luxury tour operator Scott Dunn.
As a specialist in children's food Fiona was a key figure in the press launch of Tesco's Eat Happy campaign, and alongside the launch she also filmed a number of teaching resources for schools.
She's an ambassador and blogger for The Children's Food Trust, the largest charity of its kind on the UK, providing an advisory role to government.
Fiona also frequently turns her hand to broader food reviewing and was recently seen on Channel 5's The Restaurant Inspector.
Fiona has demo'ed at an array of food festivals. As a food / travel journalist she's been commissioned to write for publications ranging from The Independent to The London Evening Standard through to Delicious and Olive magazines (ask for cuttings).
Fiona's background is in radio presentation and voice-over with live broadcast experience that spans working with Chris Evans back in the days of Virgin radio through to presenting her own syndicated music show on the (former) TLRC network – growing an audience of around a million listeners nightly. You can listen to her radio demo here)
This mini film is a snapshot of her work.
Fiona has three children and lives in Somerset.


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Voting for this year's Golden Joystick Awards, the only gaming award show where the winners are chosen by the gamers, is open now.
2016 marks the 34th year of the Golden Joysticks, which feature 25 voting categories including 'Best Original Game', 'Best Visual Design' and 'Studio of the Year.'
21 of the voting categories are chosen by gamers themselves, while the winners of an additional 4 will be chosen by an expert panel consisting of members of Future PLC's editorial teams.
For the first time member's of the public will be able to attend the event, which takes place at the indigo at the O2 Arena. Tickets are available from the Golden Joysticks EventBrite page.
- Public voting is open now on the Golden Joysticks website.


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Vivitar Vivitar Series One Digital SLR Camera/iPad Sling Backpack - Small
Don't get caught without something you need to catch that perfect shot! The Vivitar photo/SLR/Tablet sling backpack has plenty of space for everything you need on-the-go. With multiple different sized zipper pockets and padded protected area for your fragile devices, this sling back is the ultimate life-saver. The bag is extremely lightweight yet durable, and specifically designed for any traveler!


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Guest Posts
Here is a list of guest posts I've written for other blogs. The most recent are listed first.
Do you have a blog you'd like me to write for? If so, please contact me here. Thank you!
One Remarkable Thing About Personal Healing[Having Time]
Enjoy the Little Things: 11 Ways to Find the Sacred in Everyday Life
Positivity: Change Your Language, Change Your Life[BalanceinMe.com]
Breathe Your Way to Brain Balance
Why constantly changing course as we learn from mistakes is the most direct way to get there
How Facebook Can Be Your Blog's Best Friend[VirginBloggerNotes.com]


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Friday, February 27, 2009
I have been having trouble getting my dishes spotless. Heck I've been having trouble getting my dishes to come clean, period. Every time I would open the dishwasher it looked like it had never been turned on in the first place.
After reading this
I decided to give this homemade dish washing detergent recipe a try.
All you need is:
Baking Soda
White vinegar
Add equal parts of Borax & baking soda to your cleaning compartments
(about 2 tablespoons)
Vinegar to your rinse department
Even though I was cautiously optimistic upon trying this out. I have to say that I am downright amazed at the results. My dishes are actually clean. No flakes of leftover food, no sticky residue or film. No longer do I have re-wash the dishes by hand. Yippee! I 'm saving water! I'm saving time and money!Plus these products are natural which is good for my septic tank.
Lissa can go green!
Thanks Ms. Prissy! Everyday I learn something new and this week it was from you!
Labels: dishwasher, prissy green, recipe
6 comments:
- PrissyGreen said...
so glad to help! thank you for posting! makes feel all warm and fuzzy! not moldy warm and fuzzy, like teddy bear warm and fuzzy, just wanted to clear that up!
- February 27, 2009 at 10:42 AM
- sues2u2 said...
Hey Lissa. That's a great recipe (& Ms Prissy of course). Now I want to know if you can help me w/ why my whites are starting to look dingy. We're on a well/septic as well. Any ideas?
Cute blog you have here!
- March 1, 2009 at 2:00 PM
- Anonymous said...
I love finding things like this out! Thank you!
- March 1, 2009 at 5:54 PM
- Sock Monkey said...
Howdy! Dropping by fromm sits. Meet Sock Monkey. He is looking forward to meeting new people.
Sock Monkey
- March 2, 2009 at 8:18 AM
- Sock Monkey said...
Following your blog!
- March 2, 2009 at 8:19 AM
- Kathleen W. said...
That's great to hear that it worked. I miss our dishwasher from our last apartment, but when we redo our kitchen, it's the first thing I want. I'll have to keep this recipe in mind.
Thanks for commenting on the one on my blog!
- March 3, 2009 at 4:17 PM


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Chargeable assets: legal and beneficial ownership
Capital Gains Tax is charged under TCGA92/S1 (1) on the disposal of assets, but it is important to bear in mind that the legal owner of an asset is not necessarily its beneficial owner and that it is beneficial ownership (not legal ownership) which the tax principally follows. The point is particularly important in relation to partnership assets, see CG27000, family and matrimonial or civil partnership property, see CG65300+, and real property (land) generally, see CG70500+. Detailed instructions are given at CG34300+.


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ersi marina's workroomis always open to the public, even when I am not in. Sometimes I need to sleep.
Thursday, 26 September 2013
a simple walk and a techno-dreamer
14 comments:
The little dots of blue are indeed delightful ... and the greens and pinks, and the sun ... delicious!ReplyDelete
And Columbina has life well worked-out...Replies
Oh, yes! Colombina is a princess and has not one care in the world :) Thanks, Charlton.Delete
Looks like you've been Roy G Biving, Ersi! Such a rich mix of colors...love the blue dots! I am dreaming about relaxing but have to keep on writing!!ReplyDeleteReplies
Roy G Biving indeed! I love autumn, it's like a gentle caress of nature. I'm sure your writing effort will bring forth great occasions for relax and fulfillment, hold on in there!Delete
gorgeous colours Ersi ... wish I had been there in the autumn and not mid winter/spring! Was beautiful where you live - must be totally engaging now. I have been busy looking out for you on my doorstep xReplyDeleteReplies
Maybe next time you can make it in autumn? With my apartment flood-free and great opportunities to walk around and enjoy those sweet autumn colours?Delete
My beaming-over-to-Australia efforts have been unsuccessful, I'll have to try the conventional flying-over methods. On my witch's broom I mean :)) XX
What wonderful colours Autmn offers - they are such a delight to the eye, they are almost made to distract you from the fact that what follows next is winter! Love the colours - thank you!ReplyDeleteReplies
Thank you, Fiona! I'll try to take more pictures in October, when the vineyards turn red before losing their leaves. It's quite a sight. It must seem a bit strange to you now that you are heading into summer.Delete
It sounds like you are relaxing more and enjoying fall. The colors are certainly gorgeous. A good time to be outside enjoying nature!ReplyDeleteReplies
Probably the best of times (for me, at least). I love autumn and it invites me to be out there much more than summer.Delete
Yes, I am relaxing after a long, exhausting summer. I'm in a 'if life gives you lemons, hurl them back at her' mood :)
E - beautiful images of your walk; and loved reaching the photo of the techno dreamer. BReplyDeleteReplies
Hey, Barry! Autumn is a comforting season, calms everything down and invites to reflect. And I love how my cats relax as if they hadn't a worry in the world (they don't!)Delete
Hi Ersi, thank you for your images of country side.. so similar to here in the U.S. .. beautiful nature with vivid colors.ReplyDeleteReplies
Hi Donna, happy you enjoyed the photos. Autumn has a special magic everywhere, doesn't it? I've only been to the Northeastern side of the US but I remember how much I enjoyed every walk or drive around the countyside. It was like falling in love every day. Thanks for dropping in!Delete


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Seasonal Advice About Gutters
Read articles providing seasonal advice for homeowners to preserve and protect their investment in their homes. Select from the sidebar menu for information about products, services, and other gutter news topics.
Prevent ice damage on your gutters with Heater Cap
Heater Cap keeps icicles off of your first row of shingles and help prevent ice damage and other severe winter conditions.read more


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- Research article
- Open Access
Use of mRNA-seq to discriminate contributions to the transcriptome from the constituent genomes of the polyploid crop species Brassica napus
© Higgins et al.; licensee BioMed Central Ltd. 2012
- Received: 20 October 2011
- Accepted: 29 May 2012
- Published: 15 June 2012
Polyploidy often results in considerable changes in gene expression, both immediately and over evolutionary time. New phenotypes often arise with polyploid formation and may contribute to the fitness of polyploids in nature or their selection for use in agriculture. Oilseed rape (Brassica napus) is widely used to study the process of polyploidy both in artificially resynthesised and natural forms. mRNA-Seq, a recently developed approach to transcriptome profiling using deep-sequencing technologies is an alternative to microarrays for the study of gene expression in a polyploid.
Illumina mRNA-Seq is comparable to microarray analysis for transcript quantification but has increased sensitivity and, very importantly, the potential to distinguish between homoeologous genes in polyploids. Using a novel curing process, we adapted a reference sequence that was a consensus derived from ESTs from both Brassica A and C genomes to one containing separate A and C genome versions for each of the 94,558 original unigenes. We aligned reads from B. napus to this cured reference, finding 38% more reads mapping from resynthesised lines and 28% more reads mapping from natural lines. Where the A and C versions differed at single nucleotide positions, termed inter-homoeologue polymorphisms (IHPs), we were able to apportion expression in the polyploid between the A and C genome homoeologues. 43,761 unigenes contained at least one IHP, with a mean frequency of 10.5 per kb unigene sequence. 6,350 of the unigenes with IHPs were differentially expressed between homoeologous gene pairs in resynthesised B. napus. 3,212 unigenes showed a similar pattern of differential expression across a range of natural B. napus crop varieties and, of these, 995 were in common with resynthesised B. napus. Functional classification showed over-representation in gene ontology categories not associated with dosage-sensitivity.
mRNA-Seq is the method of choice for measuring transcript abundance in polyploids due to its ability to measure the contributions of homoeologues to gene expression. The identification of large numbers of differentially expressed genes in both a newly resynthesised polyploid and natural B. napus confirms that there are both immediate and long-term alterations in the expression of homoeologous gene pairs following polyploidy.
- Transcript Abundance
- Oilseed Rape
- Perl Script
- Unigene Sequence
- Genome Version
Polyploidy or whole genome duplication (WGD) has occurred multiple times throughout the evolutionary history of plants. It has long been recognized as a major force in angiosperm evolution, plant speciation and diversification [1–3]. Polyploidization is both an ancient and an ongoing evolutionary process [4, 5] and has played a role in the adaptation of a wide range of crops to different environments by generating phenotypic variation. Polyploids are generally divided into two categories; autopolyploids from duplication of the same genome; and allopolyploids from hybridization of two diverged genomes with subsequent genome duplication. These distinctions are less clear in paleopolyploids. Soybean [6] and maize [7] are considered to be paleopolyploids having been formed between 10 – 15 Mya. Both show evidence of diploidization, an ongoing process by which a newly formed polyploid becomes stabilized, involving the loss of duplicated genes, thereby returning the genome to a diploid-like form [8]. Both potato and alfalfa are derived through autopolyploidy, while wheat, oat, cotton, coffee and oilseed rape have allopolyploidy in their evolutionary history. Trapogoyon is a young allopolyploid species that has formed multiple times over the last 80 years [9] and so offers the opportunity to study a natural allopolyploid which is sympatric with its parental species [10]. The success of newly formed angiosperm polyploids is partly attributable to their highly plastic genome structure. Recent studies have documented rapid and dynamic changes in genomic structure and gene expression in plant polyploids. Much of the functional plasticity in polyploids is correlated with gene expression changes at transcriptional and post-transcriptional levels. Such gene expression changes are controlled largely by epigenetic mechanisms [1, 2, 11].
The Brassica species include an important group of vegetable and oil crops and their genomes have complex evolutionary histories. A major focus for research has been Brassica napus (oilseed rape). This is an allopolyploid species formed by the hybridization of progenitor species Brassica rapa (which contributed the A genome) and Brassica oleracea (which contributed the C genome). The Brassica species in general, and B. napus in particular, provide an excellent system in which to study the impacts of polyploidy and the processes by which genomes subsequently stabilize. B. rapa and B. oleracea are closely related, having diverged around 3.5 Mya [12]. The B. napus types cultivated as crops arose from natural polyploid formation, probably during human cultivation, i.e. less than 10,000 years ago. Genetic mapping studies confirmed that the progenitor A and C genomes are essentially intact in natural lines of B. napus and have not been substantially rearranged [13]. It is also possible to make newly constructed ("resynthesised") polyploids in the laboratory by crossing B. rapa and B. oleracea accessions and doubling chromosomes (typically by chemical treatment). Song et al.[14] used resynthesised polyploids to study genome evolution in the early generations after polyploidization and demonstrated that polyploid species can generate extensive genetic diversity in a short period of time. Pires et al.[15] were interested in the ability of polyploids to possess novel traits that are not present in their diploid progenitors which has allowed polyploids to successfully enter new ecological niches. Focussing on flowering time they showed evidence of chromosomal rearrangements and changes in gene expression, which partially explained the phenotypic variation in B. napus. The mechanisms for chromosome stability and diploidization in polyploids remain largely unknown but a study of 50 resynthesised lines of B. napus showed that in the first generation (S 0 ) of resynthesised B. napus, genetic changes are rare but cytosine methylation changes are frequent, whereas in later generations (S 5 ) genetic changes are much more frequent, but the S 0 methylation remained fixed [16]. The genetic changes observed in resynthesised B. napus are not random and there is evidence that many are the consequence of homoeologous recombination [17]. Recent cytological investigations including a S 10:11 generation showed that changes in copy number of individual chromosomes increased with successive generations; they showed gross chromosomal rearrangements and that dosage balance mechanisms enforced chromosome number stability [18]. There is much interest on how these genetic and epigenetic changes contribute to changes in gene expression. Transcriptional changes are likely to be a critical component of polyploid evolution as they can contribute directly to novel phenotypes. Most studies have compared gene expression in resynthesised polyploid lines to expression in their parents to provide evidence of additive or non-additive gene expression. According to the "additivity hypothesis", newly-synthesized allopolyploids are supposed to display mid-parental expression patterns. Many exceptions are found in resynthesised allopolyploids e.g. Arabidopsis[19], Senecio[20], Brassica[17], Triticum[21], and Gossypium[22], suggesting that the differential regulation of gene expression is a common feature of plant allopolyploids. Although the phenomenon of non-additive expression in inter-specific hybrids and allopolyploids is now well described, the underlying mechanisms are still poorly understood. Recent studies have used statistical methods to predict the contribution of each parent to gene expression in the polyploid using genome-wide microarrays that are not able to distinguish between expression of homoeologous pairs [17, 22]. The "additivity" hypothesis was confirmed using comparative proteomics on newly resynthesised B. napus[23]. Identification using mass spectrometry and functional categorisation of the differentially regulated proteins did not show that any functional category, metabolic pathway or subcellular localization was over- or under represented within non-additive polypeptides [24]. Comparing transcript levels in resynthesised B. napus to protein levels showed that differential protein regulation is not explained by transcriptional changes [25]. This is a complex process so another approach has been to measure transcript levels of homoeologous pairs of genes, but not transcriptome-wide. For example, Dong et al.[26] showed a complex pattern of differential expression in response to abiotic stress in both natural and resynthesised allopolyploid Gossypium hirsutum using SSCP-cDNA gels to distinguish homoeologous pairs of 60 genes. Also, Chaudhary et al.[27] used a mass-spectrometry-based SNP detection technique to measure allele- and homoeologue-specific contributions to the transcriptome of diploid and allopolyploid cotton and showed that 40% of homoeologues were transcriptionally biased in at least one stage of cotton development. Development of a method to measure genome-wide differential expression of homoeologous pairs using transcriptome sequencing in both synthetic and natural polyploids would contribute to our understanding of this complex process.
Next generation sequencing technologies (NGS) have opened exciting opportunities to study genomes and transcriptomes of plant species with and without sequenced genomes. Many crop genome projects are ongoing, including oilseed rape, bread wheat and banana, but many of these polyploid plants have complex genome structures meaning that producing a draft sequence is challenging [28, 29]. Meanwhile plant transcriptomics using NGS can yield much information on crops [30], including gene discovery, transcript quantification, post-transcriptional regulation and linking genotypes to phenotypes [31]. mRNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies [32]. Previous experiments, using mRNA-Seq for SNP detection in B. napus[33, 34], have proved Illumina sequencing to be an efficient method. mRNA-Seq can also be used as a method to estimate transcript abundance. The first step is to map the reads to the genome or transcriptome, and then the number of reads aligning to a specific region of the reference sequence is counted and subjected to relevant normalisation procedures [35]. It is anticipated that mRNA-Seq will revolutionize the manner in which eukaryotic transcriptomes are analysed [32] as sequencing-based approaches have clear advantages over hybridization-based approaches for quantifying the transcriptome. A range of studies comparing microarray and mRNA-Seq have consistently shown that sequencing has higher sensitivity and dynamic range [36, 37], although reproducibility has been shown to depend on the type of sample studied [36, 38, 39] and a recent study has shown that technical variability is too high to be ignored [40].
We previously developed a set of 94,558 Brassica unigenes, by assembly of all available Brassica ESTs, and used this set for the design of a Brassica microarray [41]. We used this for the analysis of gene expression in resynthesised B. napus lines ("B. napus 1" and "B. napus 2"). These resynthesised B. napus lines shared the same parental combinations of B. rapa (R-o-18) and B. oleracea (A12DH), but from reciprocal crosses. The Brassica unigenes were assembled using parameters that enabled the separate assembly of transcripts of paralogous genes within each diploid Brassica genome (as these differ by ~15% at the nucleotide level) but the co-assembly of transcripts of homoeologous genes (which differ by only ~3%). The surface-bound oligonucleotide probes of the microarrays were designed to regions that differed most between unigenes (typically 3' untranslated regions), so discriminate well between unigenes representing paralogous genes, but they have no capability to discriminate between homoeologous genes.
In the present study, we report the development of methodology for using mRNA-Seq to quantify transcript abundance in polyploids, with estimation of the relative contributions of homoeologous genes. As a proof of concept, we analysed mRNA from reserved aliquots of the same ground leaf samples taken from the resynthesised B. napus plants used in our 2009 microarray-based study. We show, inter alia, that mRNA-Seq can be used successfully for both qualitative and quantitative analyses of gene expression and for the apportioning of transcript abundance to A and C genomes in both resynthesised and natural B. napus.
Results and discussion
Comparison of microarray and mRNA-seq for transcript quantification
Summary of read alignments
Naive reference
Cured reference
No. sequence reads
No. sequence reads mapped to reference
Aligned reference sequence
Average depth over aligned reference
No. unigenes with mapped sequence reads
Total sequence mapped to naive reference/Gb
No. sequence reads mapped to A genome
No. A genome unigenes with mapped sequence reads
No. sequence reads mapped to C genome
No. C genome unigenes with mapped sequence reads
Aligned reference sequence
Average depth over aligned reference
Total sequence mapped to cured reference/ Gb
Increase sequence mapped to reference
/ %
B. napus 1 rep1
Resynthesized (B. rapa cytoplasm)
B. napus 1 rep2
B. napus 1 rep3
B. napus 1 rep4
B. napus 2 rep1
Resynthesized (B. oleracea cytoplasm)
B. napus 2 rep2
B. napus 2 rep3
B. napus 2 rep4
Ningyou 7
Aphid Resistant Rape
Modifying the reference sequence to improve read alignment
The B. rapa-cured and B. oleracea-cured unigene sequences were simply combined to produce the cured reference sequence, which thus comprised the A and C genome variants for each unigene (189,116 unigene sequences in all). Sequences (as intact 80 base reads) from the 4 biological replicates of B. napus 1 and B. napus 2 were re-mapped to the cured reference. The results are shown in Table 1. Starting with an average of 30.5 million raw reads for B. napus 1, an average of 8.7 million reads aligned to A genome versions of the unigenes (57,078 unigenes)and an average of 8.3 million reads aligned to the C genome versions of the unigenes (57,191 unigenes), this represented a total number of reads aligning to the cured reference of an average of 17.1 million reads with a mean depth of coverage of the aligned reference of 27.7-fold, which is an increase of an average of 4.7 million reads (38.6%) compared to the naive reference. Similar results were obtained for B. napus 2, which showed a 37.8% increase in the number of mapped reads. These results confirmed that the curing process was highly successful in enabling the mapping of a greater proportion of sequence reads from the resynthesised B. napus. The B. rapa-cured and B. oleracea-cured unigene sequences will be of value for exploiting mRNA-Seq for transcript quantification in these species. The large number of IHPs differentiating the unigenes in the cured reference (385,789) also provides the opportunity to estimate the relative contributions to the transcriptome of homoeologues in the A and C genomes.
Estimation of the relative contributions of homoeologues to the transcriptome
The mapping to the cured reference of sequences from the four biological replicates of B. napus 1 and B. napus 2 results in the assignment of each sequence read to the unigene with the fewest mismatches. Where MAQ finds equally probable matches, the read is assigned randomly to one of them. Consequently, the cured reference provides the opportunity to estimate the relative contributions to the transcriptome of homoeologues based on this apportioning of reads, but with an anticipated underestimation of differences between the contributions of homoeologues in the A and C genomes where there are tracts of identical sequence in the corresponding unigenes in the cured reference.
To test whether our method for the apportioning of transcript abundance to genomes is also applicable to natural B. napus, we generated leaf mRNA-Seq data from five cultivars representing differing crop types. The sequence datasets consisted of between 23 and 29 million raw reads, the mean Phred-like quality value at base 80 was Q26.8 and the mean position at which the quality value declined below Q30 of 60.8. The sequence reads were mapped to both the naive reference and the cured reference. As was observed with mRNA-Seq reads from the resynthesised B. napus, more sequences were mapped to the cured reference than to the naive reference, as summarised in Table 1. However, the increase for natural B. napus of 27.8%, was lower than that observed for resynthesised B. napus (~38%). The difference is likely to be due to the curing process having used sequences from the precise progenitors of the resynthesised B. napus, resulting in optimum read mapping, whereas the actual progenitors of the natural B. napus types are unknown. We observed consistent (but not significant) biases in the proportions of reads mapping to the A and C genome versions of unigenes for both resynthesised B. napus (the majority, 51.2%, mapping to the A genome) and natural B. napus (the majority, 51.5%, mapping to the C genome). This may be a consequence of the transcriptome sequence of B. oleracea A12DH being more similar to that of the C genome ancestor of natural B. napus than the transcriptome sequence of B. rapa R-o-18 is to that of the A genome ancestor of natural B. napus, which has been observed previously for some regions of the genome [46].
Analysis and functional classification of homoeologous unigene pairs showing differential transcript abundance
Our investigations have shown that mRNA-Seq represents an excellent approach to the analysis of both qualitative and quantitative transcript abundance in B. napus. The increased sensitivity and dynamic range compared with a corresponding analysis using microarrays, along with the good (but far from perfect) correlation of transcript abundance quantification is consistent with the results of previous studies conducted using organisms with simpler genomes. As the cost to sequence a sample is now comparable to the cost to conduct a microarray analysis and software tools are available to manage and mine mRNA-Seq data, we conclude that mRNA-Seq will frequently be the method of choice.
Our studies have shown that mRNA-Seq data can be used to overcome a key problem in polyploid species: the presence of homoeologous genes with very similar sequence being transcribed from each genome. By "curing" the reference unigene sequences so they more closely represent the sequences of the two genomes contained in B. napus, we have been able to allocate, on a genome-wide basis, transcript abundance to the individual genomes. Although limited to regions of unigenes in which inter-homoeologue polymorphisms occur, such regions are in the majority and the approach identified many homoeologous transcripts with differential expression from the two genomes. This approach should be generally applicable to polyploid species, so long as diploid representatives of the constituent genomes are available to support the curing process. Moreover, the identification of large numbers of genes with such transcription biases, in replicated experiments, demonstrates that stable homoeo-allelic variation for transcript abundance is common in B. napus.
This method also enabled us to successfully study differential expression of homoeologous genes across a range of natural B. napus crop varieties. For many genes, the ancestral profiles have been partitioned between the homoeologues in a similar pattern, despite many generations of independent evolution. This is consistent with observations in other polyploids, such as cotton (Gossypium) [26, 27] and Spartina[50], that there are both immediate and long-term alterations in the expression of homoeologous gene pairs following polyploid formation. Functional classification of genes differentially expressed between homoeologous gene pairs showed enrichment for classes not associated with gene dosage effects, consistent with the notion that such gene classes can show allelic variation for transcript abundance that might, itself, represent a class of molecular marker.
Growth of plants and preparation of RNA
The B. napus 1 and B. napus 2 RNA samples were extracted from stored aliquots of the same ground leaf tissue used for the microarray experiment as described by Trick et al.[41]. Five varieties of natural B. napus were selected to represent the main crop types: Tapidor, Ningyou 7, Altasweet, Ceska and Aphid Resistant Rape. The plants were grown and the RNA extracted using the same experimental design, growth and harvesting conditions as described for the B. napus 1 and B. napus 2 plants by Trick et al.[41].
Illumina RNA-sequencing
The sequencing libraries were prepared using the Illumina mRNA-Seq kit (RS-100-0801, Illumina Inc.) as described by Bancroft et al.[33]. Each library was run on a single lane for 80 cycles on the Illumina Genome Analyzer GAIIx. Illumina base calling files were processed using GERALD to produce a sequence file containing 80 base reads for each sample in FASTQ format. The Illumina FASTQ format was converted to Sanger FASTQ format before further processing.
Mapping of reads to the reference sequence and generation of read counts using MAQ
MAQ version 0.7.1 [42] was used to align the 80 base Illumina reads to the 95 k naive reference sequence following the protocols described in the online documentation (http://maq.sourceforge.net) and adopting the default parameter values. MAQ pileup text files were generated from the MAQ binary map files. The Perl script tagcounter.pl (Additional file 1) was used to count the number of reads aligning to each unigene by accessing the pileup files, outputting a count and calculated RPKM value (reads per kb per million aligned reads) for each unigene.
Comparison of unigene expression measurements obtained using the Agilent microarray with illumina mRNA-seq count data
Data for the four biological replicates of each of B. napus 1 and B. napus 2 was obtained from the Agilent 60-mer oligonucleotide microarray experiment already published by Trick et al.[41] and available from the GEO repository, accession number GSE15915. Unigenes were called expressed, marginally expressed or non-expressed using the Agilent microarray. A similar classification of unigenes was carried out using the Illumina count data. Expressed unigenes were defined as having one or more reads aligning to the unigene in all of the 4 replicates; non-expressed unigenes were defined as having zero counts in each of the 4 replicates, the remaining unigenes were not classified. The statistical programming language R [51] was used to compare the lists obtained from both methods using Set Analysis. Above-background quantitative signal values were obtained for the Agilent microarray. These were correlated with the RPKM values (normalised transcript abundance) obtained using Illumina mRNA-Seq for the unigenes which were called expressed using both methods.
Preparation of the cured reference sequence
Libraries prepared from B. rapa and B. oleracea RNA samples were each run on two lanes of the Illumina Genome Analyzer GAIIx for 80 cycles. The FASTQ files from the two lanes were combined generating a total of 46,120,559 reads for B. rapa and 49,268,765 reads for B. oleracea. The 80 base reads were split into two files, each containing a set of 40 base reads using the Perl script illumina_split_read.pl (Additional file 2). The 40 base reads were used separately to cure the naive reference sequence to an A genome version and a C genome version, described as follows. Using the Perl script cure_cycle_split.pl (Additional file 3), the 40 base reads were aligned against the naive reference sequence to produce a map file. The map files generated by alignment of the first and second sets of 40 base reads were merged using MAQ mapmerge and a consensus sequence generated. The Perl script, cure_refseqs.pl (Additional file 4), was used to cure the naive reference using the consensus sequence. This process was iterated over six cycles after which there was no significant gain in alignment efficiency. On each iteration, bases were replaced in the reference, where these differed from high quality consensus bases called by MAQ (i.e. contributed by a read depth greater than 3, with quality values greater than 40). This process resulted in the production of an A genome and C genome version of the naive reference, these two sequences were compared at each base position using the Perl script compare_sequences.pl (Additional file 5) to give a list of positions within unigenes where the base differed in the two sequences. The cured reference sequence was constructed by combining the two 'cured' reference sequences, thus creating a reference sequence containing both the A and C variants of each unigene.
Mapping of reads to the cured reference and the apportioning of reads to the A and C genome
B. napus 1 and B. napus 2 samples were re-aligned against the cured reference using MAQ. When a read maps equally well to multiple positions, MAQ will randomly pick one position, thereby distributing reads evenly between the A and C genome versions of the unigene where the sequence is identical. Using the pileup files, the Perl script ACtagcounter.pl (Additional file 6) was used to generate a count of the number of reads and corresponding RPKM value, separately for the A and C genome version of each unigene.
RPKM values for the number of reads aligning to the A and C genome versions of each unigene were analysed in R. Following the principle of Occam's razor, the simplest distribution, Poisson, was fitted to the paired RPKM values across the four replicates of each cross using a Generalised Linear Model (GLM); with the paired structure of the data captured algebraically in the design matrix Chi. However, evident over-dispersion in the data necessitated the use of a quasi-Poisson link function. That is, the variance and mean regression functions can be obtained from a Poisson GLM [52] but the dispersion parameter, Phi, is not fixed at 1 but left unrestricted and subsequently estimated from the data. Hence, we obtain the same estimates of coefficients as for a standard Poisson, but inference becomes effectively over-dispersion-adjusted. The resulting p-values were subjected to Benjamini-Hochberg adjustment for multiple testing; and we regarded adjusted p-values < 0.05 as significant. Using the same statistical analysis, we compared the five B. napus cultivars with the aim of identifying unigenes which were consistently differentially expressed across all five varieties.
Identifying biological functions of differentially expressed genes
BLASTN hits in Arabidopsis (p ≤ 1.0E-30) were found for 62,383 of the 94,558 brassica unigenes (90,864 unique sequences [41]). The top Arabidopsis hit corresponding to each Brassica unigene was used for functional analysis. Functional classification was carried out using the agriGO web-based GO analysis toolkit [47]. Arabidopsis genes (corresponding to each unigenes) were input into the Singular Enrichment Analysis (SEA) using the 43,761 unigenes with IHPs as a customised reference background (15,173 corresponding Arabidopsis genes). The Fisher's Exact Test with Bonferroni-adjusted p-values was employed in the SEA analysis using the "complete GO" ontology.
Accession codes
Short read sequence data have been deposited at the Sequence Read Archive (SRA) under accession number ERA063602, except for Tapidor and Ningyou 7, which were deposited previously under accession number ERA036824.
We would like to thank The Genome Analysis Centre for generating Illumina sequence data.
This work was supported by UK Biotechnology and Biological Sciences Research Council (ERAPG08.008).
Authors' Affiliations
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I'm sure everyone is looking forward to the amazingly long weekend ahead (for those that get both Friday and Monday off). No getting up early or making lunches, woooo!! These DIY Easter eggs are a great little holiday project to do with your kids or by yourself. I picked up some foam eggs from the dollar store. All they had were the small ones but I wasn't fussy.
I grabbed some tissue paper that I had in my stash and ripped it into smaller pieces…
I then applied a layer of acrylic medium (you can use Modge Podge or Decoupage, whatever you have) then put the tissue paper over the eggs and another layer of medium over top to seal it in. I wasn't super careful applying it or anything as I love all the wrinkles and texture it makes. This is a bit of a messy job and you can't over handle them because the tissue will start to stick to your fingers.
Once they were thoroughly dry, I applied Titan Buff (which is an off white colour) with a bit of water (to make it more translucent) to all of the eggs. For this guy I had started with an old dress pattern, then the paint, then blue ink and finished it off with a stamp from Tim Holtz's line Stamper's Anonymous. I used this Little Labels set for all the eggs because the print size was appropriate for these little eggs.
This one already had plenty of colour so I just added a bit of paint and some black text. Don't you just love polka dots??
This one started with the cream swirly paper which was a bit dull so I added some yellow India Ink to it and the pointing finger from Tim's line. The circle stamp was from my basket of background stamps so I'm not sure where I got it.
This one just had plain pink paper so I added a bit more paint to it then the others to tone it down a little and the black text.
Here they are together in a vase with some raffia. I will put a couple of these on my Easter dinner table on Sunday to spruce it up a bit.
An easy and fairly quick (except for the drying time in between stages) project you can get done in time for Easter and they won't stink up the house if one accidentally rolls under the couch and is forgotten. Plus, you can keep them for next year!! I would love to see pics if you try them yourself.


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A friend of mind recently opened her very own brick and mortar store.
I'm most proud of her. She put it together in record time and it is
just delightful to the eye and full of projects and inspiration.
(She runs circles around me in this antique business...)
Let's take a peek...
I love this view of the 1st room as you enter. Love that stone wall!
Many items in the shop received a bit of an upcycle -
like these cheese boxes...
...and who can't use a little wooden storage tote...
This industrial file cabinet has its own custom built
wooden surround (built by the owner's hubby)...
And if you're into pallet projects -
this one is just begging for some succulents...
For those who like all things French...
And of course - for us cottage and somewhat 'girly' gals -
there's plenty for us too.
Don't you just love her tags?
It's a corner shop with a great front door...
Welcome to Vintage & Co nestled in a little village in
Lancaster County, PA!
Wanna see more? Head on over to my friend's blog
and give her some love.
And if you're not local -
you can still do some shopping on her Etsy store.
Thanks for joining me for the tour! ~Joyce


text:
Today Luciano Pavarotti, the legendary tenor passed away. In reading the news, I found Pavarotti singing Ave Maria on YouTube and began to listen.
Our daughter was focused on watching The Wiggles and didn't notice, but about a minute into the song I felt eyes upon me.
Drew was sitting on the floor to my right and was staring directly at the little window with Pavarotti in the middle of a note. Over all the background noise, he determined that he wished to hear Lucianone sing. As the music continued, I took a moment to watch him, realizing that this little moment was a small bonus in life. Something I never thought I'd be able to share with my son.
Drew will never know Pavarotti (and I myself don't know much about him), and he certainly won't remember this little moment. But I will. Thank you Mr. Pavarotti.


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text: On Mon, 2009-10-19 at 08:59 +0200, Niki Kovacs wrote: > Hi, > > I've been using Conky for some time, a nifty utility to monitor just > about anything on the PC. Vital things like CPU, RAM, swap, disks, > current song playing in MPD :o) > > Here's what it looks like : > > http://www.microlinux.fr/images/bureau_conky.png > > And with more detail : > > http://www.microlinux.fr/images/conky_zoom.png > > Now I wonder... I'd really like to use that to monitor my remote server. > I know this feature isn't officially supported by Conky, but I'm right > now thinking about a workaround. Something like: OK, my server is > 'headless' (e. g.: no graphical server, nothing), but why not install > just xorg-x11-server-Xorg, then use Conky and forward it to my local > display with SSH -X ? I'm pondering this question, thinking about the > possible issues... > > ... so maybe one of you guys here has come up with some solution ? > > Cheers, > > Niki Hi Niki, You could try a local script that gets values from a server that you would like to monitor... I might suggest looking into setting up snmpd on the server and using snmp walk to probe specific values (that relate to processes/free memory). What exactly would you be looking to monitor on the remote server? Tait -------------- next part -------------- A non-text attachment was scrubbed... Name: signature.asc Type: application/pgp-signature Size: 197 bytes Desc: This is a digitally signed message part URL: <http://lists.centos.org/pipermail/centos/attachments/20091019/0ab3c19d/attachment.sig>


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- Developer / Owner: King's Cross Central Limited Partnership
- Developer / Owner: King's Cross Central Limited Partnership
- Consultant: Niall McLaughlin Architects
Tapestry brings 129 one, two and three bedroom apartments, townhouses and penthouses overlooking Gasholder Park. Niall McLaughlin Architects have achieved an incredibly considered and characterful residence, offering homes with breathtaking views over the canal and across London. The apartments offer waterside living in central London whilst also benefiting from a private, landscaped garden square in the sky designed by internationally respected Dan Pearson Studio. The building houses a multi-use game area, a multi-storey car park and the site's energy centre.
Development Team:
Developer: King's Cross Central Limited Partnership
Architect: Niall McLaughlin Architects
- Project website
- View on map
- Existing
- Borough: Camden
- Size: 125,500 sq ft
- Type: Residential


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Sam Ricketts talks to the press after the late, late defeat away to Plymouth Argyle #salop