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I wanna join hip hop with poping and best dancing level because my dream is dance plus and dancing is my dream so could u advise me when is new batch going to start
My what s up number 9540281871
Hip-hop dance is a street dance style which developed as part of Hip-hop culture. Hip-hop culture is the embodiment of four important elements: Breaking, Rapping(MCing, Beatboxing), Graphite and Djing. It started as an underground movement as a counter culture against a very racist and discriminating society. Hip-hop dance evolved in the streets of The Bronx, New York, in the early 1970s, in the African American communities.The various styles associated with Hip-hop dance are Breaking, Locking, Popping, Old School etc. What distinguishes Hip-hop from other forms of dance is that it is often "freestyle" which means it is improvisational in nature. Hip-hop dance crews and individuals engaged in freestyle dance competitions, also known as "battles".
Regularly updated schedule for all Amar Colony, Delhi classes is available at: Delhi Dance Classes Schedule.
Delhi Dance Academy – E238, Amar Colony, Lajpat Nagar4 studio
Monday, Wednesday and Friday - 5 pm to 6 pm. Enrolling at BEGINNERS Level.
Monday, Wednesday and Friday – 6 pm to 7 pm. Enrolling at IMPROVERS Level.
Saturday, Sunday – 1:30pm to 2.30pm. BEGINNERS ENROLLING
Saturday, Sunday – 5:30pm to 6.30pm. IMPROVERS ENROLLING
Call us for details.
Monthly - Rs.2500
Quarterly -
Rs.7500 Rs.6000 (saving of Rs.1500)
Half Yearly -
Rs.15000 Rs.10000 (saving of Rs.5000)
Yearly -
Rs.30000 Rs.18000 (saving of Rs.12000)
Monthly - Rs.3000
Quarterly -
Rs.9000 Rs.7000 (saving of Rs.2000)
Half Yearly -
Rs.18000 Rs.12000 (saving of Rs.6000)
Yearly -
Rs.36000 Rs.20000 (saving of Rs.16000)
Saturday & Sunday 1 pm to 4 pm at Sheikh Sarai Studio.
Monthly - Rs.4000( There are no discounts on professional Hip-hop dance course)
Hip Hop Dance is a rage among young dance students. Delhi Dance Academy conducts Hip Hop Dance classes every day of the week at its dance studios. Students are trained on diverse international Hip-Hop, House and R & B music which gives scope for different kinds of moves and styles. At this time you can either take up a 3 day a week hip-hop batch where you'll get 12 classes in a month, or a weekend-only batch with 1.5 hours on Saturday and 1.5 hours on Sunday, totaling 12 hours a month.
The term "Hip Hop" is a combination of two words, "Hip" which means current or in the know and "Hop" as in the jumping movement.
They say that Hip-Hop as a musical genre and as an artistic dance form originated somewhere in the Bronx in New York during the 1970′s. Hip Hop originally was a creative expression of the suburban African-American and Hispanic-American communities but gradually spread across communities, regions and countries and is now popular among dance enthusiasts around the world.
Hip Hop music began when DJs started experimenting with rhythmic beats in loops, then with rap vocals, hard-hitting lyrics and beatboxing. All this accompanied with deep bass, innovative guitar strumming and high-end sound effects.
While this new kind of music was gaining acceptance and popularity, particular complimentary styles of dressing, dance and art were also making way into the lives of fans. This is how Hip Hop as a dance form and Graffiti as an art form came into being. Today, music, dance and graffiti, all are essentials part of the Hip-Hop culture.
India has a big pool of a young population. With the establishment of good infrastructure and latest technology, at least in the major cities, easy access to global television and internet is now commonplace. This has brought information and knowledge about diverse cultures, global trends, world music and dance etc. within the reach of millions and millions of Indians over the past 2 decades.
Hip Hop culture in terms of music and dance started influencing the Indian youth in the early 1990′s and glimpses of Hip Hop started appearing in the Indian Pop Music industry and the Bollywood movie industry. Hip Hop music and dance have been growing in India ever since. The popularity of Hip Hop as a dance form can be seen on a plethora of dance reality shows that have flooded the Indian television in recent times.
At present, as a dance form, Hip Hop in Delhi is a mix of different kinds of professional, experimental and freestyle dancing. Delhi attracts youth from all over the country who bring their own flavour of Hip Hop dancing to the table. This has created an interesting genre of Hip Hop in Delhi which is, of course, majorly influenced by the western media, music videos, movies and t.v. shows but also includes a tinge of Bollywood and Indian contemporary. In recent years, many dance schools have come up in Delhi to teach and promote Hip-Hop. This is a good sign for the fans of Hip-Hop and for those who want to explore this new age culture.
I wanna join hip hop with poping and best dancing level because my dream is dance plus and dancing is my dream so could u advise me when is new batch going to start
My what s up number 9540281871
I want to join plz contact me and tell me wht is process for taking admission
i want to join hip hop classes. can u pls tell me when is new batch going to start.
Hello sir
I am joel mathew from north delhi. I DON'T have any doubt but.. I don't even know a a single step of dance… Fully beginner.. But as trusting… And on my confidence… Can i join on coming friday….. Sir will you clarify me that which are the batch timing of beginners…
Do you have any classes in Paschim Vihar west delhi
I want to join the hip hop classes .
And what is the timing of sunday classes & payment terms .
i am a 17 years old boy nd a very beginner about dance. i want to join hip hop classes. plz tell me about a new batch that i could join. and do tell how much time it will normally take to feel that i can dance?
Sir, I m 17ans I want to learn hip-hop for 3hour
i am a 23 years old boy nd a very beginner about dance. i want to join hip hop classes. plz tell me about a new batch that i could join. and do tell how much time it will normally take to feel that i can dance?
Please answer dear friends I m waiting for reply.
sir any centre near shahdara
sir,i m 20,ans i want to learn lyrical hiphop
Hi am based in Gurgaon . Do u have centre here I Gurgaon or if you can suggest a good one to polish my hip hop skills…Thanks
Hi my life dance and I am learning dance
Like, Les twins,ragav juyal,sumedh mudgalkar,please help me
I want to join hiphop classes but I have time only sat Sunday
hi there. I actually dance a bit(freestyle) here in my college. But still i feel a lot uncomfortable to practice dance with others or in front of others. so just need a boost to dance without any second thoughts. So, will it be good to join the class for one month or so?!
I want to learn the Hip Hop dance . I am a beginner right now. I like to do the dance but I need the right place where i can learn it. There is any place near noida where i can join . Currently I am working in MNC company. So it's hard for me to join the classes in weekdays.
Please give me some information , So I can join the classes as soon as possible.
Thanks in advance for giving your precious time.
Please reply ………
Can I join
Hi DDA,
I love dancing and i want to learn it but im 22 i hope it is not too late to learm dancing
I like urban hip hop but confused what to choose as justim Timberlake is my favourite and i like his dance form also
So suggest me urban hip hop would br good ?and i am hope I will feel comfortable as I'm bit shy
Hi DDA,
I love dancing and i want to learn it but im 22 i hope it is not too late to learm dancing
I like urban hip hop but confused what to choose as justim Timberlake is my favourite and i like his dance form also
So suggest me urban hip hop would br good ?and i am bit shy also i hope ill be feel comfortable there as im not so good in dance im just a beginner
Is there any upcoming hip hop classes at 7 pm or after 7 pm? Due to my work, it will be impossible to join before 7 pm
Hlo I want to join ur dance class..
But I don't know hip hop..
I wanted to learn dancing at teenage but due to my studies I couldn't pursue my passion , but now I am in my final year of graduation and I have plenty amount of time to learn dancing .
I am 22 and I have no prior experience in dancing , I don't know even a single step .
Do you have any suitable batch/course for me ?
I am interested in learning Hip-Hop dance.
I wnt to become a best dancer and i wnt to become a famous,so there is any class which in future I'm selected in any dance realty show..plzz tl me…
N m waiting for ur rply
Dear Sir,
I'm a Hip Hop Dancer, Looking for a platform for make career success. I also know Melody Creation. Kindly Suggest me. I'm waiting for ur reply, It's very Important for my life so please help me. Thanking You!
I'm Smriti and I so wanted to join dance academy. I'm not that good dancer but my passion is to dance and want to learn more. Can I join the dance academy? I'm 19 year old college student. Can I join for more than 1 dance form? Is there any audition for the enrollment in the dance academy? Please Notify me with your response. Also I have sent a mail!
sir am from Nigeria how i wish i can join your dancing group u're performing well thanks.
I want to learn a professional Poping & Locking course. what should i do.
I form Delhi
I have just graduated from high school. I have been a part of the school dance team ( Hip Hop). I want to learn b- boying . Which course should i opt for and when is the earliest i can join ?
I don't know anything about dance but I want to know can u give me a deep information..
Hi….I am sanjay shama….I think mujhe hiphop dance sikhana hai…….how many rupees per montj
I am namrita arya (19).. I have done events as a dancer and done many forms in it.. bt m little bit cnfuse either i join salsa or hip hop .. in beginners or not in beginners..
glad to hear about this academy.
i want to know when should i join,i mean is there a session like april-march or jan-dec?
and one more thing is that
will u give a certificate of dance after completing the class?
I'm a bit confused whether to join intermediate or advanced classes.
How different are both?
Want to join DDA . Hw can I reach u
I am very intrested in dancin and i love dancing I want to dance like Tiger shroff what do i like them dance but i am a little bit thicker and i know very little about dance
Dear. Sir ma hip hop sekhna chata hu please sir hama batay Khan cekha please sir my mobile no 9560456946″8909441419. I love hip hop please sir
I want to join these classes. I am very passionate about dance! I will do anything, any step.
Anushree, I understand your passion for dance. To take this passion ahead, I will recommend you to try the Professional batches that are available at our academy. To know more about them call me on 9811555359. I will tell you the enrollment and the trial procedure.
Keep your passion alive!
Sir my name is Aman Agrawal. I am little fat and I am 19 years old. I want to learn break dance like professionals. I am very interested in dancing but I don't have prior training in it. Can I achieve dancing skills like professionals?
Hi Aman!
Age and weight is no bar to start learning dance at Delhi Dance Academy! If you have genuine inclination towards dancing then there is nothing that can stop you to do so. We do have specific professional Hip-Hop batch available at our studio at Amar Colony, Lajpat Nagar-4 by the name of Xtreme Hip-Hop. To know more about it call me on 9811555359.
Make your feet and yourself happy at Delhi Dance Academy!
I am looking for a weekend batch for Hip hop in Gurgaon. Any new batch starting soon?
Hi Karishma!
We do have a weekend batch for Urban Hip-Hop in Gurgaon. The timing of the batch is from 10 a.m. to 11:30 a.m. on both Saturdays and Sundays. You can give me a call on 9811555359 to know about the enrollment procedure.
Hi DDA,
I want to learn Urban hip hop. Please tell me the program and fee structure to learn urban style.
Hi! I want to be a professional dancer and want to learn hip hop. Please suggest in this regard. How much time will it take please?
I am planning to join your Hip-Hop Classes. I just want to know that what will be the strength(Number of Student) of a batch.
Hi Gaurav, you can definitely join our Hip-Hop batch
As per the studio strength we keep maximum 14 students at one time.
For Hip-Hop we have 5-6pm (M,W,F),6-7pm (T,T,S) and 12:30-2:00pm Sat,Sun.
Fee is Rs.2000 per month.
Hi! Can you please tell me the fee for one month?
Hi, The fee is Rs.2000 per month for Hip-Hop classes.
For Hip-Hop timing is 5-6pm Monday-Wednesday-Friday.
Hi! I want to join academy.
Hi! You are most welcome to join our academy.For hip-hop we have 5-6pm Monday-Wednesday-Friday.
can u please start your classes in east delhi please please please…..i want to join this institute asap.
Dear Akshay, we do not have a branch in East Delhi and the expansion plans are all in South Delhi. If free on weekends, please come to the South Delhi center, we have lots of options for you. Call me on 9910222770 for details. Best regards, Arjun
I want to learn hip hop from the very basic. I live in South Delhi. I will be able to join only weekend classes.
Please Suggest.
Thanks and Regards,
Dear Bhavya, join the 12:30 – 2pm Saturday and Sunday batch. It has just begun and is the right place for you to start! Arjun 9910222770.
Hey! Ma'am can you please give me private classes, have come from Kashmir and want to learn Hip-Hop.But only problem is that I can't give your fee.I am
struggling,Can you please help me?
You can definitely get private classes from the alligned instructor. But charges are little high for such classes, Rs.1000 per hour. As far as discount is concerned we do help needy students by giving them a scholarship underwhich fee reduces to 50%.you can visit academy whenever you want or call Arjun (9910222770) or Anant (9953835088).
Hi sir/ma'am
i want to learn hip hop from basic level i live in ghaziabad so is there any batch in morning available for me???
i want to join as soon as possible!!!!
Hi Shubham! We do have one new batch starting in the first half on weekend(Sat,Sun).Timing is 12-01:30 pm.
You can come tomorrow for trial.
Please suggest your hip hop centre in Gurgaon ?
Hi! I am afraid to tell you that currently we don't have any branch in Gurgaon but we are running few batches for hip hop on weekends,
just try if you can manage.Basic batch started yesterday at 2pm.
Sir,actually I want to ask u that the hip hop dance classes are started, if I join soon I will be able to learn the basics means from starting or not?
Hi Deepak!
We just have started a new basic hip hop batch last week,6-7 pm Monday,Wednesday,Friday.
You can join that batch to learn from basic.
Sir mai dance sikhna chahta hui par ek problem hai ki mai two thousand math nhi de sakta 3000 rup kamata hui so plz solve this problem sir 8873914609.
We have scholarships for needy students.
For that please come to the academy and meet Anant,Arjun or me.
Hi. I am very much interested in learning the Hip-Hop and locking popping genre. I have some basic skills required for dancing and have performed in college as well. What I expect to learn from your class is proper Hip-Hop may be right from the basics. I want to know the timings of both weekdays and weekends.
Hi Pratik! We understand that Hip-Hop is a quite technical form of dance,so please rest assured that you will be taught all the technical bits and the course will be very structured when you join DDA. I feel it will be good if you start from the basics. During weekdays the basic batch runs from 5pm to 6pm Monday to Friday and during weekends it's 2:30pm to 5:00pm Saturday and Sunday.
The fee for both batches is the same – Rs 2000 per month or Rs 5400 for 3 months Level A Certificate course or Rs 9600 for the 6 month Level A + B. Regards, Nandita (08376095792)
Thank you Nandita wil be contacting soon in this regarding…
When Shall I be There 2 learn Dance…
Me from VADODARA CITY, GUJARAT..
i WANT TO LEARN dANCE..
so please send me details abt your classes including direct contact to Choreographer Sir Anish Kaul..
Thank you..
Rply SooN..
Dear Vikrant, please call our landline number 011 41012909 and ask for Anish Sir, we can't provide his direct number without his permission. Regards Arjun
Hi Admin,
Dance is in my blood…..I am good at Hip hop..but not very good….I was with Shiamak Davar as student for 3 years..I left few months back due to some reason. Actually i am planing to go for DID audition this year. I need some one to train me on a particular one/two song. I am ready to pay the charges.
I have heard a lot about your classes & i trust u so i want you to train me.
Kindly please help me out & let me know if you can?
I will be glad to be trained by you before going for audition.
Please do reply.
Abhinav Singh
Hi Abhinav, we have a 10 hour one-to-one masterclass package where one of our lead choreographers and someone from the management meet you, plan what you're going to do for the audition depending on your dancing skills and proficiency. Then we start working on your dance and give you 10 private classes of 1 hour each to prepare your choreography with the aim of doing very well at various dance auditions. Before the end of March, we have a 20% discount going on for anyone willing to book 10 audition masterclasses, which means you get it for Rs 8000 in stead of Rs 10,000. Regards Arjun 9910222770
Hi. I am very much interested in learning the Hip-Hop and locking popping genre. I have some basic skills required for dancing and have performed in college as well. What I expect to learn from your class is proper Hip-Hop right from the basics coz in most of the classes I have been taught simple bollywood dance in place of Hip Hop. I hope this time I'm at right place
In weekdays probably i can't join coz of office so I would like to join weekend batches.Please provide your no. I will be joining sooner.
Hi Ankit, what you need is Raman Pratap Singh's weekend 5:30pm to 7:00pm pure hip hop batch. Raman is a purist and swears by the dance form, he loves hip hop. The video on this page is a specimen of his talent. Come take a trial class and you won't be be disappointed. 5:30-7pm, Sat and Sun for Rs 2000 a month or Rs 5400 for the entire level A. Regards, Arjun 9910222770
Thanks for the reply Arjun
we are organising a street dance competition in our university jamia millia islamia and we want hip hop dancers from your academy to participate in it . to register see this facebook page http://www.facebook.com/pages/Street-Dance/341507122616547?fref=ts
Dear Madhur, unfortunately we'll have to decline this offer because of very little time to prepare and because of insufficient information regarding the event and prizes.. but keep us posted the next time and we'll send in a team regards Arjun
Hi … i want to learn dance …. but d problem is i m a v shy person and even non dancer … so can yu plz help me … i want to improve myself …
No problem Simran, many people who first come to DDA are shy and we provide a nice atmosphere where no one judges you, in fact all of us try to make you feel comfortable. You will learn dance and also lose the hesitation. Feel confident to come for a trial class. You will have fun! Arjun (9910222770)
heyy !!
im 18 yrs old doing b-tech
i have to do something new along with studies
I haven't learn't dance so i want to learn dance
need to join delhi dance academy
plz tell me what's a procedure to join
Devesh – Thank you for your interest in DDA. First choose a time you want to join, then call us and check if there's space available. We are starting a new hip-hop, popping locking batch from 3-4pm by Anish sir, which will start from the basics. The fee is Rs 5400 for 3 months or Rs 2000 per month. You will need to bring a passport sized photo, fill an application form that you will get from our office and pay the fee to begin classes. Regards Arjun
respected sir
i want to learn locking and popping style of dacing or in short the dance form which dharmesh sir does can u guide me in this direction and provide me some details.
and i am 19 yrs old i do dance at home and try to do diff steps.one small problem is that my weight is 70kg.
Tejas, are you free from 5:30 to 7pm on Saturday and Sunday? Choreographer Raman Pratap Singh is taking excellent popping and locking classes at these times and the 70 Kg weight is not a problem as long as you have the undying desire to learn! Arjun
Hello DDA!
Don't temme you've just one center?
Nothing for west delhites?
Dear Riju, thank you for your interest in Delhi Dance Academy. We have 4 centers but all in South Delhi, but we should have something in West Delhi before the end of the year Regards Arjun
Hi sir,
I want to join Saturday and Sunday classes.
I want to learn Hip-Hop and Bollywood.
I'm 27 years old. Can I learn Hip-Hop and Bollywood at this age? I have learnt to dance a little bit at home, but I want to learn properly. Would you please tell me if my age is okay to learn dancing?
Dear Anurg,
On Saturday and Sunday we have hip hop from 5:30-7pm and Bollywood from 5:00-6:30pm. You are most welcome to join these batches, although they have conflicting timings and therefore you'll be able to join only one at a time.
27 is very old, it won't be possible! Lol.. kidding, it is a brilliant age to start and we have people in their 50s and 60s doing dance as well. Don't worry and come for a trial class.
Call 011 41012909 or call me personally on 9910222770 to book your trial class.
Best regards,
Sir! I would like to know whether I can enroll in any batch of your's to learn hip hop for Basic Level on weekends.
Hi Saurabh, there is a new hip-hop, popping and locking batch by Raman starting fresh from the basics. To enroll in this, call me on 9910222770 and I'll book you in Regards Arjun
hey sir
i m vry pasionate abt dancing nd i really wanna join acdmy it will be a plessure to me to be a past of yr acadmy …………………….i wanna know everything about it…………………make me aware by al yr activites
age-16 yrs
nangloi delhi-41
Dear Divya, thank you for writing to us. We can answer all your questions if you call us please. My number is 9910222770. We have 4 hip hop batches on week-days and weekends and the fee is Rs 2000 per month. You can also come for a trial class on Sat or Sun from 5:30 to 7pm. Regards,Arjun
I want to learn dance pla tell address
I m a beginner, i wan to learn contemporary. So, plz give me details of contemporary style.
(batch timing,fee,day,etc.)
Hi Sanjay, we have 2 new contemporary batches. Sat Sun 1pm to 2:30pm and Mon, Wed, Fri from 6pm to 7pm. Fee is Rs 2000 per month and Rs 5500 if you pay for the 3 months course together. Regards Arjun 9910222770
Hey admin.
itz prince…..
I would lyk to join lyricle hip hop classes.
Wat would be my timing & fee.
Thank you…..
Hi Prince, we have hip-hop by Anish sir, which includes lyrical hip hop plus we have urban hip hop from Sumit. Please call me to discuss your exact requirements. Arjun 9910222770
Hi DDA
And one think also sir that what is the time period of pure hip hop dance is there is any classes between 4pm to 7pm??????
Hi Crystal, Level A course is of 3 months duration. There is a class from 5-6pm from Monday to Friday.
Hello Crystal, we have 4 Hip Hop batches that you can join – some on the weekdays and some on weekends. The center is in Amar Colony (close to Moolchand metro station). If you think that is commutable, then please call us and book a trial for Rs 299. Regards Arjun
I am 11 yrs old.Now my exams are coming and they get will over in March.So do you have any batches for me after March.I love dance Hip-Hop dance style the most but I know little bit of that dance.Would you tell me little bit about this dance form.
Hi Nidhi! Good to hear from you. We have a 5-6pm hip hop class from Monday to Friday where we have some youngsters like you. Do contact me on 9910222770 when you're ready to join and good luck with your exams ! Arjun
i am big fan of hip hop and similar dance forms. but i dont even know d of dance. is there any class for beginners like me form the verrryyyy basic level????? can you please forward me the details of class timing ,fee structure and location of DDA??
Hi Shivangi, we have beginners' hip-hop batches for complete beginners. Don't worry, it's the first step that counts and you've already taken that by writing to us. Fix a trial class for Rs 300 in one of our beginner hip-hop batches by calling me on 9910222770 and you'll see how simple it is to get started. In 3 months you'll find yourself a basic/intermediate hip-hop dancer. Regards, Arjun
hello sir, i would like to ask i m a 6th class student nd i hav a competion in my school on 4 dec. .i want to do solo dance so i want to ask is there available fr solo only fr a mnth only ??
Hi Tejas, the only option is to take private solo classes at our studio in Amar Colony. Do you want to learn solo for an upcoming show or just for fun? It will be Rs 1000 per hour in our studio. Regards Arjun 9910222770
I wish to learn dancing preferably hip hop. I am 22 yrs and don't know how to dance although I love watching people dance and always wanted to be a part of it. Is it possible for a person like me to learn dancing. Is there any kind of age barrier or something. Please let me know. I can only join weekend classes as I'm working now.
Hello Mahesh, we have beginners' Hip Hop classes for guys and girls who are learning hip-hop for the first time. You can learn, and we've also just started weekend classes from 5:30pm to 7:00pm. Do come and speak to us or come for a trial class for Rs 300 (this will be adjusted with your fee in case you join). Or call me on 9910222770 (Arjun). Thanks
hi sir ….my name is abhilash patil livingin south delhi ..and i'm intrested in hip hop….but problem is that i have only 3 months time that too in june of next year to august so pls if u have any classes for those three months , then pls inform me ….. well one more question is , can u just tell me how much time it will take to learn hip hop, imean for a beginner , becoz i'm one of them
Hi Abhilash, I'm not sure about the hip-hop batches that we'll have at that time. I think it's best if you bookmark this page and save my number (9910222770) and give me a call back a few weeks before you want to start. It depends from person to person, but you should be good at basic hip hop steps in 3 months' time.
Hello I would like to learn the hip hop dance. Is there any enrollment going on in december???
Hello Rachita, we are not sure about the December batches just yet as it is only October, but I know that we will have about 4 hip hop batches going on in December. Please bookmark this page or call me on 9910222770 a couple of weeks before you want to join please. I'll give you up-to-date information then. Best regards Arjun
hello sir my self sumit panwar n i want to learn hip-hop and b-boying…nd i m from panipat…sir muje abhi dance me kuch nhi aata h mera interst bahot h bt meh koi style nhi pata..so sir aap muje time table and address bta skte ho…….sir muje dance me bahot sucess krna hai…
Hi Sumit, the address is on the contact us page and time table as of 3rd October 2012 is : 8-9 am pure hip hop 5 days a week, 3-4pm, 5-6pm and 8-9pm 5 days a week pure hip hop. Please drop in anytime and we'll be happy to teach you. All these batches are for beginners and are taking enrollments. Please call to book your place.
join dance class
Hello Pawan, You're welcome to join out hip-hop classes which take place 5 days a week at the following times:
1. 8-9am
2. 3-4pm
3. 5-6pm
4. 8-9pm
Please call to enroll.
I really want to learn hip hop and I can't find a good place near where I live. Can you please send me the address and details of DDA?
Hello Anwar, here you go: http://www.delhidanceacademy.in/contact-us/
Hi DDA ,
I am a working person n i also love dancing ; actually i am fond of dancing. My feet move whenever and wherever i hear music of any kind. I have also seen your styles that u teach but i need to learn Contemporary, jazz, hip hop & B-boying. Do you have a nice package with a combination of all these.
I work in shifts which changes as my boss want so dont worry i will try to mange the time. Kindly help in the above case…..
Dear Gaurav, you can join batches of contemporary jazz and hip hop at the same time. As of Sep 2012, we don't teach b-boying anymore because of risk of injury. Please call me on 9910222770 to find out times and to register. Arjun 9910222770.
hi DDA,
i would like to join for hip pop and salsa classes but would prefer it on weekends. Is der any batch available on weekends for the same. i would also like to know if we have to get a parter for ourself if we want to join salsa classes.
Dear Sanjana, hope you're well We have Salsa from 3.00pm to 4:30pm on Sat and Sun. You don't need a partner for Salsa as you're usually able to find someone at DDA. We don't have hip-hop on the weekends as of now, I'm sorry. Please call us to register for Salsa. The fee is Rs 2000 per month.
Hey its J, can you tell me, Are b-boying hip-hop and Hip-hop different from each other or the same.?
i wanna learn western hip hop dance so let me know about classes timing. Lajpat nagar-4
Hello J, Hip Hop and B-boying are different. Hip Hop is like break dance and B-boying is stunts. We don't teach B Boying, but have 2 batches of pure hip hop that you can join. Both are from Monday to Friday 1. 5-6pm and 2. 8-9pm They are both conducted in the Amar Colony branch, in Lajpat Nagar 4. Fee is Rs 2000 per month. Please call me to enroll at 9910222770 – Arjun
Hello Admin,
I am basically working during weekday and the only time that I have is during weekends. I have gone through the post and I see that there are lot people who would want to join but during weekends. Can you please consider this and try to arrange classes for people who can come only during the weekends.
I would want to learn Hip Hop and would surely join if you have weekend classes. Please consider
Hello Wilson, thank you for writing to us. We now have a Hip Hop batch on the weekends that you can join. 7pm to 8:30pm on Saturday and Sunday. Please call me on 9910222770 to book your place. The batch starts on the 3rd of November and we're taking enrollments now. Regards, Arjun
I'm a working guy and I want to learn hip hop during evenings in the weekends. After 7pm will suit me best, please let me know if there's something.
Pawan, we now have a Hip Hop batch on the weekends from 7pm to 8:30pm on Saturday and Sunday, starts on the 3rd of November and we're taking enrollments now. Regards, Arjun 9910222770
i wanna join the hip-hop ciasses………….
Call us and book Jyoti
hey ! i wanna join hip hop classes could anyone plz tell me when and how can i enroll.
Hey…I love dancing but the main problem is my age….i m 28… can I learn any form of dance at this age??
Gia, you can, people start in their 40s and 50s. You're a baby and the best time to start is now
for september i should say ..
I was just wondering what classes you have for small children (5 years of age) if any thanks
Hello sir,
my name is pawan.i want to learn hip hop dance,could u plss tell me fee structure nd timing of the class….
hey there…
im a rapper n i thought it would be cool if i learn n start doin bboyin also…!!
would you help me out with the exact address of your institute or somethin… i'm really lookin forward to it n i wanna join ASAP…
i want 2 learn krumping with popping .it,s possible
i wnt join a best dance group. so plsss… send me ur adress whr i cn come & give audition.
hou r u??????
i wana learn hip hop dance
but i go in school and i leave in
bahadurgarh (haryana)
so plz help me
how r u ??????????
hay i wana learn hip hop dance
but i go in school and i leave in bahadurgarh (haryana)
but i realy learn dance plz help me
Actually I'm working from Monday to Friday and I want to learn hip hop also. Could you please help me out about dance class timings and fee structure?
Hi Dinesh, we have a Bollywood cum Hip Hop batch from 5pm to 6pm on Saturday and Sunday. Please come to the academy on Saturday at 5pm to enroll. Thanks.
HELLO Sir
I want to learn dance but i dont know a thing about it because i havent really danced in my life.im 18 yrs old n i really want to learn it.could you tell me about the dance forms available in your institute as well as their fees and timings.
Hello sir, my name is Amit and i love dance and I want to join a dance group for events in your Academy, is that possible?
Hi DDA,
I want to learn Hip Hop. Do you guys teach at your Rajouri Garden studio? I want to join the weekend batch.
Please reply asap
i want to know that there is a rajouri garden centre on ur website…. but u are mentioning only lajpat nagar centres….???
i want to learn dance, i used to do the dance but due to some personal problem i had to leave it but now i want to come back on my track, i m living kapsheda delhi , i m working person my age is 22 and i want to make my carrer in dancing
Hi Vishwa,
We're teaching lots of different dance forms like Hip Hop, Bollywood, Contemporary, Salsa etc. at Delhi Dance Academy. You are welcome to drop in any time and join in. You could call us for more information.
Ph. +91 9953835088
Dear DDA,
I am interested in learning B – Boying dance classes but my main concern is timing, so is there any batch which start after 07:30 pm & if it is possible any batch on weekends after 03:30 pm….
Hi Satinder,
We do have a batch for Hip Hop and B-Boying going on right now but unfortunately the timing is a little different. Its from 3:30 to 5 pm every evening from Monday to Friday. Please try if you can manage these timings and let us know.
Ph. +91 9953835088
i love hip hop dace but i can't effort your fee
i want to learn hip hop .as i m a college going student will get time only in evening ,is there any batch which is conducted at evening and what will be the fee structure
Hello Bulbul,
We have batch for Hip Hop and B-Boying from 3:30 to 5 pm every weekday. The fees is Rs.2000 per month. There are other batches later in the evening which have a mix of Bollywood, Hip Hop and Freestyle. Please let me know if you need more information.
Ph. +91 9953835088
hey! dda
i wud lyk to know wen r d new batches starting from??
do you hav any batches startin in july 2012??
Hi Tanvi,
We have lots of batches going on for all kinds of dance forms. These are continuous classes and you can join any time. Please just give me a call and I'll tell you the exact timings.
Ph. +91 9953835088
i wanna learn hip hop dance …but i dont know how to dance i never dance before…so i'm looking for a dance class who helps the beginners..!
Hi Purushotam,
No problem, lots beginners join our classes. Presently, there is one batch for Hip Hop and B-Boying which happens every weekday from 3:30 to 5 pm. You can just give me a call and drop in for the same.
Ph. +91 9953835088
Do you have dance batches for Hip-Hop early in the morning on weekends? Let's say around 7 or 8? If not, then do you have late dance classes on weekends?
Is there any way, I can come for trial dance classes?
Hi Prateek,
We don't have Hip Hop in the mornings on weekends but generally there are some Bollywood Freestyle batches in the evenings. We could arrange for a trial class for you if there is space and if you call us in advance. Please let me know.
Ph. +91 9953835088
hiiii ….DDA
i wanna to learn hip hop dance and be boying too ….i would love to join ….plz give me any contact no.for mor information ……..:)
Hi Lokesh,
This is Anant and you could call me on 9953835088 for more information. We have Hip Hop going on every weekday from 3:30 to 5 pm.
i love dancing though i m not a very good dancer. so i want to learn some dance form. i want to participate in some dance show… (lol, not sure if that is ever going to happend or not). i have not made up my mind, which dance form i want to learn. i live in faridabad. which is the nearest branch? i have my training on weekdays so weekends will be better.
can u give some links to ur performances videos?
Hi Sid,
We will be happy to have you at Delhi Dance Academy. You can choose from any of the following dance forms and start off: Bollywood, Hip Hop, Salsa and Contemporary. Presently, we do have batches for Bollywood, Salsa and Contemporary on weekends at our Amar Colony center which will be the closest to Faridabad.
Some of our videos can be seen on http://www.delhidanceacademy.in/video-gallery/.
+91 9953835088
hi i m very fond of dance but i dont whether i m a gud or bad dancer bcoz i never dance in public………..means i m a rookie so i want to learn from basic of hip hop ……so i need ur suggestion……….
Hi Atul,
No problem. We have very good instructors who should be able to help you. You can start with Hip Hop and B-Boying which happens every weekday from 3:30 to 5 pm or any Bollywood-Freestyle batch in the evenings.
Please call me for more information.
+91 9953835088
hii dda,….
i want to know that u also provise trial class to new comers?????
Hi Hemant,
We could provide a trial class if there is space in the respective batch and if you call us and book in advance.
Ph. +91 9953835088
hello dda i want to learn dance hiphop bt i dont know single thing abt it……means i want to learn from starting….plz help me i realy passionate abt it…..
Hello there,
We have very good instructors who will be able to help you out. A batch for Hip Hop is going on these days. It happens from 3:30 to 5 pm every weekday from Monday to Friday. Please come to our center in Amar Colony or call me for more information.
hello dda
im 20yr old im looking forward to join dance classes in hip hop n free style etc starting from basic i.e for beginners………im realy passionate bt i never dance anywhere in public or elsewhere it just keep it in house now i want to open my talent…..so plz suggest ur advice…….
Hi Atul,
Good to see that you're so passionate about dance. We teach Hip Hop, Bollywood, Salsa, Jazz Contemporary and Zumba at Delhi Dance Academy. You are welcome to join any of these classes and I am sure you'll enjoy.
Please call me for any more information.
+91 9953835088
i would like to join for hiphop 3 days per week ..where can i contact you?
Hello Minam,
We do have a batch for Hip Hop. It happens Mon-Fri 3:30 to 5 pm and you could opt for 3 days a week as well.
Please call me for more details.
hi my name is mannu . i learn hip hop plz tell me wht the charges and do you have any center in laxmi nagar or nera
Hi Mannu,
Unfortunately, we don't have a center in that area as now. But we are looking to open more centers and details will come up on the website as soon as that happens.
+91 9953835088
do you have any current batch available for hip hop??
Hi Priya,
Yes, we do have a batch for Hip Hop going on. The classes are every day from Monday to Friday from 3:30 to 5pm.
You can call me once and drop in to have a look at the class.
+91 9953835088
Please tell me about the next batch for hiphop is gonna be start, I want to join it…and also the timing and the fee structure.which is nearest branch of fur academy as m residing in vikaspuri…
thank u !!
Hi Swati,
We're having classes only at Amar Colony, Lajpat Nagar at the moment.
Hip Hop happens every weekday Monday to Friday from 3:30 to 5 pm. The fee is Rs.2000 per month.
+91 9953835088
hey i am a student… just got over with my exams and i just have two months of vacations i.e june n july after which my college starts again.. i would love to join your academy for learning hip hop. and since i have got only two months i would like to utilize as many days possible. so is it possible to get an alternate days or three days a week sort of a thing? and also leme know about the centre nearest to mayur vihar i.e east delhi..
waiting for your reply..
thank you
Hi Mridu,
Hope you already got all the information you needed.
Hip Hop happens every day from Monday to Friday from 3:30 to 5 pm. 3 days a week can also be worked out. Please call me in case you need any more information.
+91 9953835088
hi, i want to know is there any current batch for hip hop.
Hi Priyanka,
There's a batch for Hip Hop with classes every weekday Monday to Friday from 3:30 to 5 pm. Please call me id you want to join.
+91 9953835088
Hello, I'm 19, & i have vacations during June and July..
I would like to know if you have a summer dance programme, in which i can join for maybe a month.
I'm really interested in learning dance, specially hip hop.
Please let me know if you have a similar provision for jazz.
Where is you center in South Delhi ?
Hi Nandita,
Hope you have all the information you needed by now.
We have regular batches for both Hip Hop and Jazz Contemporary. Our center is in Amar Colony, Lajpat Nagar.
Please call in for more information.
+91 9953835088
Hi, I want to learn hip hop dance . But I m beginner. Can I learn basic step of dance within 1 month.
Hi Rahul,
No problem if you're a beginner. There's a hip hop batch going on from 3:30 to 5 pm Monday to Friday. Join in and I am sure you will learn a lot.
Please call me if you need more information.
+91 9953835088
do u have any center near punjabi bagh?
Hi Shivan,
No center in Punjabi Bagh unfortunately. We will keep updating our site for new centers.
+91 9953835088
hello dda,
i am lukin forwrd 2 join classes for contemporary and hip hop, but i wnt d shifts on weekends, and evening time z grt….. pls mail me d further details asap….. coz i wnt 2 start my classes frm dis mnth only…..
Hi Pragya,
If you haven't joined us yet, let me tell you about the ongoing batches.
Contemporary has 3 batches: Sat-Sun 1 to 2:30 pm, Mon-Wed-Fri 6 to 7 pm and Tue-Thurs-Sat 6 to 7 pm.
Hip Hop takes place from 3:30 to 5 pm every weekday Monday to Friday.
Fee for any batch is Rs.2000 per month.
Please call me to join in.
+91 9953835088
hi sir
i want to b-boying and hip hop i am 14 years old i can come on 4 o clock because of my school
Hi Vikrant, you can come from 5-6 pm Hip Hop if you like. Arjun 9910222770
do u teach breakdance
Hi Vicky,
You should try our Hip Hop, B-Boying, Popping and Locking batch that takes place Monday to Friday from 3:30 to 5 pm. Please call me and I'll arrange for a trial class for you.
+91 9953835088
Hi Rahul,
We can arrange a private class for you. Please call me and we could discuss further.
+91 9953835088
do u have any centers near central delhi like CP or karol bagh?
Hello Kavita,
Sorry, no centers in CP or Karol Bagh. Will keep updating our website for new centers.
+91 9953835088
Hey DDA
I would like to know if u have a branch in west delhi. As coming daily from west delhi to amar colony will b hectic after work..
Hi Anmol,
We have only 1 center at Amar Colony, Lajpat Nagar at the moment but we're looking for more. Will post details on the website as soon as we have a center in West Delhi.
Thanks for your interest in Delhi Dance Academy.
+91 9953835088
My situation is slightly complicated. I am on vacation at the moment but college reopens during the first week of July. And I live in Gurgaon so transport is a big issue. I do dance, but I have never really concentrated on a genre. What I would like to know is that are there any workshops going on, for a short duration? And is there any transport facility provided by the academy (bus, cab, etc)
Hi Ambika,
Unfortunately, our Gurgaon center is not operational at the moment. We have classes going on only in Amar Colony, Lajpat Nagar. Also, we don't provide transport facilities.
We are in the process of expanding and will keep posting details on our website.
+91 9953835088
I am a student studying in 11th standard and i am searching for the best hip hop dance class in delhi.So, would u plz send me the fee structure and timetable for Hip Hop dance. plz tell me is this is the right place for hip hop and locking poping dance styles. plz reply me .
Hi Aman,
We have a Hip Hop specific batch taking place from 8 to 9 am every weekday Monday to Friday. Also, there is a Hip Hop and Bollywood combined batch from 3:30 to 5 pm Monday to Friday. These classes take place at our Amar Colony, Lajpat Nagar center.
You are welcome to come to the studio once and have a look. I am sure you will like these classes.
+91 9953835088
I saw that for Hip-Hop classes you have a Package 3: Weekend classes on Saturday and Sunday: Rs 2000 per month. Just wanted to know if the the timings are 9-10 or if there are other timings for this slot. Thanks.
Hi Radhika,
We're starting a weekend batch for Hip Hop shortly. The timing would be 9 to 10 am and the fee Rs.2000 per month. Please call me to register asap.
+91 9953835088
I'm turning 19yrs and i got craziness of dancing in last two years.
but I hve a big prob I don't even know the D of Dance nd wanna learn hip hop dance.
so, can u plz tell me is your dance academy helps the beginners.
if yes then mention the procedure plz.
I wanna dance!
Dear Sir
I'm belongs from U.P. here in Delhi I'm Studying in NIIT. I want learn professional Hip hop dance style. but I can't afford much more fee. can u do something for me……
Hi DDA,
Actually m a die hard fan of dancing nd i hv learnt a little bit of dancing frm youtube videos…actually m looking for any krumping or popping classes…can u help me on dis…
This is harish and i want to learn good moves of hip hop So i want to join hiphop classes.Could you plz tell me following things:-
1) The class near by Dabri-Uttamnagar.
2)Fees of Class.
4)Is your academy take the students to high competition level.
4)Is your academy give class during saturday and sunday.
Hi, I am Vijay, age 29. I am an IT professional and get very wee time to do any kind of exercise to keep my body fit. I have always wanted to dance but do not know how to do it. . I came across your dance academy, which is more or less near to my work place. I would like to know if there is any batch going at 7:00 PM, which I can join in. I want to join hip-hop and bollywood dance classes. Please let me know if you people are running any batch at that time along with the fee structure.
i want to learn hiphop…is dre ny batches startin frm this month ..or next mnth…plz tell me ..
I want to learn the Hiphop dance .. Plz suggest me the right institute.
Hi ,
I am tottally mad about b-boying and want to learn this dance form .i would love to join it and it would be better if it is near r.k.puram sec-8 .would u plz send me the fee structure and timetable for b-boying classes .
Vaibhav Tokas
Age -18
hey me and my friends want to join ur classes will u plzz inform which age group u all train and the adress as well..
thank u!!
me and my sister (16 and 12) want to join hip hop classes. We live near vasant kunj. Is there any studio near vasant kunj or saket? and what are the charges like?
i want to learn hip hop in duration of may – july .can u let me know about the duration of learning .and its cost and do u have any center near old delhi?.
Do you have any centers in Gurgaon.?
Hi Sahil, yes we do Map and Address at http://www.delhidanceacademy.in/contact-us/
hi sir,
i love dance and i want to learn it.Cud you tel me about ur dance centers near by tilak nager and also tel me about ur fee and time
i want to know is there any dance centres near Hauz khas in new delhi ….would love to join it ….
hey, dda
i m anuj, i m a student of b.tech in panipat .n i looking for krumping classes.so is there any class for me on weekend..n also tell me the fee structure.
i want to lear hip hop dance can tell tell me how much it cost
for learning….,,.,,
waiting for ur message
I want to learn complete Hip-Hop including all the stunts. My preference is weekend classes. So tell me the fee structure and classes routine. And are there any special discounts for college students ??
P.S. a friend of mine want to lose weight. So should she go for aerobics or dance can serve the same purpose as aerobics.
hoping for a positive reply ASAP.
Hi..I want learn hip hop..but to make it clear that I havent learnt dance ever and am not sure even if I can learn dance..
I put up in Faridabad and work in Gurgaon..So travelling from Gurgaon to delhi and then back to faridabad is not feasible.Plus my office timings are pretty much long..So do you have any centers in Gurgaon.?
Hi..I want to learn hip-hop(to be clear that i haven't learnt dancing ever and i am not sure even I can learn dance or not)..I am working in Gurgaon and put up in Faridabad..my work timings are pretty much long and travelling to lajpat Nagar after work is not feasible for me..do you have any centers in gurgaon.?
hi DDA,
my name is shilpa.. i love dancing and i wanna learn hip hop .. but i cant join now coz of my exams .. is dre any batch startng from may !! i would love to join
Hey Shilpa, good to see your passion. We're here for you whenever you're ready. Good luck.
Hello DDA,
I am looking forward to join dance classes for hip-hop, b- boying or contemporary. Do u have these dance forms taught at your rajinder nagar branch?? And is it possible for weekend classes for the above dance forms??
I would also like to know about the 1-to-1 dance classes.
Thank you
Hello Mayank, yes we have all the ones that you've mentioned. We have a really good 4-5pm hip hop batch. Why don't you drop in at the academy one day and we'll enroll you in a batch. Arjun
Hello Arjun Sir,
Plz tell me when and what time can i come on a weekend and know all the details regarding the dance class.
hello sir,
how want to learn hip-hop dance
please tell me fees & timing
& other branch is near by dilshad garden
Hello Vinay, the fee is Rs 2000 per month and we have various timings 5 times a week. We have nothing around Dilshad Garden for now I'm afraid Vinay
I m vry pasonte abt dncng …… Cud u tel me ur dance centers near by patel nagar… Nd abt ur fee nd timetables
Hello Nishant, we're looking for more dance centres, but at present we have nothing in Patel Nagar, I'm afraid.
Hi, I'm interested in joining hip hop dance classes. But do you have any branch in North Delhi. As it would be very inconvenient for me to come to south delhi.
Dear Vinny, we have lots of Hip Hop batches in South Delhi, but none in North Delhi yet, I'm afraid. We are actively looking to start new centres though.
could u plz tell me about your classes and fee structure for freestyle dance?
I learn to Hiphop dance
I learn to dance
Hello DDA,
I am very passionate about dancing and also have been performing since my school days. I am keenly interested in Hip-Hop, B-Boying & Freestyle Dancing. After a lot of surfing I found this a suitable place to land upon. So can you mail me the complete details of the courses. My only problem is that I am free during weekends. Please suggest me something asap.
Dear Deep, thanks for writing to us. On weekends we have batches of Bollywood dance and Salsa only. However, we are starting a Hip Hop batch for weekends from the 11th of Feb 2012. Please come in to our studio at Amar colony (details are given at the contact page) between 6pm and 9pm tomorrow (Saturday the 4th of Feb 2012) and speak with the instructor Rajat, who'll be able to custom make something for you.
Good luck with your pursuits
hey! i love to dance and was looking for some hip hop classes, could u tell me about your centres in west delhi,if any?
Dear Devyani, is Rajinder Nagar suitable for you? We have a centre there, very close to Rajinder Nagar metro station. A few other dance classes in west Delhi are coming up, of which I'll keep you posted. Many thanks for your interest. Please reply here.
hi DDA!
I'm very fond of dancing but not good at it. Its my hobby so i would like to give it a try. Not professionally, but just to fulfill my desire. I like all kinds of dance (freestyle), most importantly locking n popping. I'm a student from Manipur (15yrs) and will b coming to Delhi for 1 month, so can u please tell me if there are any classes just for a month or two? I would love to learn as much as I can. Can u please tell me the fee structure and procedure to be undertaken for admission also? Thank you.
Dear Alice, it's good to see you desire to learn. We have a batch where we teach Bollywood mixed with Hip Hop. One month is not a long enough time to master a dance form, but a month will give you a better dance body and help you move to music better. Call us when you're in Delhi and come in to our studio for a trial.
The fee structure is given on the fee page on this website and the procedure for admission is for you to get a form of ID, a passport size photo and your fee – very straight forward
Good luck
want to learn completly not only hip hop but each n every form of dance except clasicals and folk,,,,,,,,,,,, ,,,,,,,,,,,,,, i want to learn be boying too …….. so plz suggest me the right teacher ,,,,,,,,
Dear Tarun,
Thanks for getting in touch. We have a dance class by our extremely talented choreographer Rajat where he teaches Hip Hop, B Boying and Bollywood in the same batch. The idea of having a batch like that is to make a dance form that is accepted these days and is in great demand – we think that when you add a little bit of Hip Hop and B Boying to Bollywood, you create a dance form that attracts more attention and is better accepted in the modern Indian media.
Come over to the academy any day at 6 pm, 7 pm or 8 pm and meet Rajat, he'll be able to discuss your exact requirements and place you in an appropriate batch depending on your dancing proficiency.
Hi I am 25 year old. I haven't still learn't dance. Now I just wanna learn dance. To learn dance I have some positive points such as I know Yoga and I have flexible body.
Raj, 25 is a young age and even though it's never too late to learn, you are in perfectly the right slab to start. Having a flexible body is a great advantage and our trainers will take care of the rest.
Give us a call to arrange a time to come in to one of our dance studios and we'll start from there.
Hey DDA,
i'd like to join the dance classes during my semester break(approx 2 months).Can u please let me know the procedures 2 b undertaken for admission??
Dear Nabasmita, the procedure for admission is – you have to bring a form of ID, one passport size photograph, fill in our application form and pay 1 month's fee in advance to join. The fees are payable at the start of every month.
We do offer a free trial class where you can get a feel of the class, meet other students and chat with the instructor. This is a no obligation class and I'd recommend you come in for one at your earliest convenience.
Best regards
Hi there.. I have always been fond of hip-hop dancing. My working hours are constraints but I love to dance & would like to learn hip-hop dancing.. What's the fee structure?
Hello Mona,
We cater to a lot of working professionals who have busy schedules and so we have many batches to choose from for all kinds of dance forms. There are many batches for Bollywood and Hip Hop combined and one dedicated batch for B-Boying. Please check our detailed time table and fee structure on http://www.delhidanceacademy.in/time-table-fees/.
Do call me regarding any batch that might be convenient for you to attend. I will try and arrange a free trial class for you.
Looking forward to seeing you at Delhi Dance Academy
Ph. +91 9953835088
Hi DDA
Any 1-to-1 hIphop classes? Weekends for example?
Hi again Philippa
Yes, we could arrange private 1-to-1 Hip Hop classes as well. Would you like to call me on 9953835088 to discuss further?
Hi DDA
Danced hIphop a while back and really want to restart. Had a weird experience when I first arrived in India and attended a 'hiphop' class at another dance school that was totally Bollywood (interesting but not my thing).
So just wondering… Is your hIphop more hIphop, or more Bollywood? If more Bollywood, are 1-to-1 classes an option?
Hi Philippa,
Lots of our students also prefer a mix of Bollywood with Hip Hop and so we also have many Bollywood and Hip Hop combined batches throughout the week. However, we also have a dedicated B-Boying class that happens on all weekdays from 9 to 10 pm, which might suit you.
The detailed time table for all classes in given on http://www.delhidanceacademy.in/time-table-fees. I could arrange a free trial for you if you'd like to come, have a look and make up your mind. Please call me on 9953835088 and let me know what you think.
Hi, I want to dance but not professionally. I dont dance but I want to learn it. Is it easy to learn the dance who does not know the dance. I just want to learn the bollywood and locking pocking. Is it easy to learn and perform dance. Please reply , Just want to join few class in a week. Waiting for your reply.
Hi Sandeep,
Lots of students join our dance classes as a hobby or as a daily fitness routine. Its not that only those who want to dance professionally can join dance classes.
How fast one learns depends on individual ability and practice. It will be ideal if you take a few classes and find out for yourself. I am sure you'll enjoy
Please call me on 9953835088 for more details. I could also arrange a Free Trial class for you.
Best regards,
I want to learn Lyrical Hip hop
Hi ,
I am a young working professional and would like to know more about your hip hop dance classes .i would love to join it . my only constraint is that i work in shifts . would u plz send me the fee structure and timetable for above mentioned course .
sheel bhadra parmar
Age -26
Hello Sheel,
Thanks for your interest in Delhi Dance Academy
Hip Hop Dance classes are conducted throughout the week. You can check the time table and fees on http://delhidanceacademy.in/time-table-fees/.
The studio is located at E.1, Basement, Amar Colony, Lajpat Nagar IV, New Delhi.
You can call me on 9953835088 and I could arrange a Free Trial class for you.
i want to join hip hop classes. can u pls tell me when is new batch going to start.
Hi there,
Hip Hop happening from 3:30 to 5 pm Monday to Friday.
+91 9953835088
DDA remains one of Delhi's most sought after dance academies - The Hindu
Delhi's Top 5 Places to Learn Hip Hop, Popping and Locking - HT City, Hindustan Times
Belly Dancing Classes That'll Help You Ace The Art - HT City, Hindustan Times
Top 5 Places to Learn Bollywood Dance in Delhi - HT City, Hindustan Times
Delhi's Best Places to Learn Dance - Delhi Times, Times of India
Zumba at Delhi Dance Academy, I must confess, leaves me panting and exhausted - Business Standard
Shaadi Poori Filmi Hai (Article on Wedding Choreography) - TFL Magazine
Namaste India Dance Workshops - Editor's Pick - Timeout Delhi Magazine
The Top Place for B Boying, Hip Hop, Funk Jazz and Zumba - Hindustan Times main newspaper
Gymnastics Classes by Arman Safa Khan - Timeout Delhi
Shall We Dance? Director Arjun Sandhu interviewed by HT Mint
11 Unusual Things To Do In Delhi by About.com
Top 10 Things to Do In Delhi by Guardian UK
Latest Schedule of all Batches in Delhi is available at: Latest Dance Classes Schedule Delhi
Latest Schedule of all Batches in Gurgaon is available at: Latest Dance Classes Schedule Gurgaon


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Apartment in EsteponaStylish frontline beach apartment located in the sought after Guadalmansa Playa resort between Marbella & Estepona, featuring direct sea access, 24h security service, a heated outdoor swimming pool, sauna and on site gymnasium. Designed for demanding Corporate and Celebrity VIP clients, this luxurious apartment is very well appointed and finished to the highest standard, with marble floors throughout and generous sized rooms. Fabric wallpaper, feature walls and attractive lighting make the apartment both cosy and luxurious. There are top of the range appliances and fittings in the kitchen and bathrooms, together with a warm and cool air conditioning system, with under-floor heating in all bathrooms. Much of the furniture has been specially commissioned for the apartments to match the fabrics and the decor and to complement the layout and dimensions, all available at preferential rates.
• 2 Bedrooms • 2 Bathrooms • 175m2
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subset: commoncrawl


text:
Grief changes our experience of life. When we are grieving, enter into a crucible of transformation. Grief forces us to journey through a threshold into an unfamiliar terrain. And in this terrain we find ourselves, standing alone with our suffering, wondering which way to go.
The On the Loss of My Parents series is a tribute to my parents, Bruce Alger (1918-2011) and Vi Alger (1919-2010). It is the origin and foundation for my project, "Aging in the Second Half of Life." On the Loss of My Parents is also an example of how transformative writing can cultivate healing, self-renewal, and understanding that is good for self and others at the same time.
Inside Grief
Grief defies analysis. There are no stages of grief. We can identify common characteristics and qualities of grief, but the journey we are compelled to take is personal and unique. I cannot really "know" another person's grief, and no one else can really understand the nature of mine.
I think of grief as a threshold because I felt as though I moved through a point of no return in life when my parents died. The death of a loved one tears a threshold right in the middle of our own reality. And we fall down on the cusp of that opening, weakened and on bloodied knees.
What once was will never be again. Grief gives us permission to express our heartache and sadness at the brutal side of life.
I also believe that grief is a journey in which we have become lost and alone, and are trying to find our way back into life again.
Grief does not merely come to visit for a while, it takes up a permanent residence inside. We do not "heal" our grief, but we do learn from it and accommodate its presence. All experiences of grief are unique. There are no standardized stages for grieving.
Grief tears a threshold into our lifestyle, and demands that we begin a journey into the mystery of the rest of our life.
When we grieve our spirit is invaded by severe feelings of sadness and anguish. There is a biting sharpness in our grief that hijacks our sensibilities. And we feel as though we are drowning in an agonizing swirl of melancholy that offers no reprieve.
Initially, grief caused me to fall down. I was hurt and unable to move soon after my parents had died. My body, mind, and spirit were wounded. I lost my way in life. I was confined in the midst of my freedom. I lost my mobility in life.
When life has beaten us down, we have to pick ourselves up and find a way to put one foot in front of the other again. Grief makes movement painful and uncertain. The reason for this is clear to me now. I could not simply pick up the pieces up and return to life as it was. That is not the goal. Grief demands that we stand up again and journey into the unknown.
We must hold a conversation on the edge, a conversation with our own anguish.
Grief changes everything about us and the world around us.
The Artistry of Bereavement
Grief is an inhospitable learning environment.
I held imaginary conversations with my parents after they had died. They were now on the other side of the Great Mystery. What would they say to me now?
I kept looking for them. A ghost of what they once were. Perhaps their spirits would appear to my physical senses. If only I could talk to them now. I want to know where they are and if they are ok. Are they at rest? What does that even mean?
They continue to have a large presence in my life. Sometimes, I feel them when I am in the presence of nature. Maybe our energy really does return into the Earth, and flows back into Life again. They are everywhere now, amongst the trees, hills, and landscapes.
Clichés attacked me on all sides. What would my parents want for me now that they are gone? The answer is obvious, yet unsatisfying. Another part of me feels I am being too dismissive. Grief makes the need to know relentless and intolerable.
There are things about life we are not meant to know.
There are things in life we can never know.
And when we can't know, we must embrace a fierce sense of creativity. When we can't understand, we must attempt to discover things we didn't know existed. This is the crucible of the creative fire within.
Mom and Dad…
Though I can no longer feel your touch
Except through the breath of my spirit;
You subtly inhabit a private space within
And remain as faithful to me as the day I was born.
I don't know why I intuitively turned toward poetry. I just did. And quality aside, I realized that writing poetry was the beginning of a real and authentic conversation with my grief.
I got up from the ground. I was still wounded. But I tried to walk again for the first time.
There is a poetry waiting to be discovered that is hidden inside our pain.
Creativity is the beginning of a merciful release from our suffering.
The Poetics of Grief
For me, the ideal response to grief is the creative expression of that grief. This implies an artistry of grieving and a poetics of grief. Moving into grief creatively is an essential frontier of learning and a powerful point of no return in life.
The poet lives and writes at the frontier between deep internal experience and the revelations of the outer world. There is no going back for the poet once this frontier has been reached; a new territory is visible and what has been said cannot be unsaid.
The death of a loved one is a fierce and primal call to attention. Grief ensnares us in spiritual vertigo, mental confusion, and emotional agitation. Mourning overwhelms our body, constricts our mind, and focuses our spirit.
When we look out into the world through the lens of grief, many of the priorities we once held appear greyed out and unimportant. We intuitively know that we have started a process of transformation, but words still fail to describe our experience.
This unknowing is precisely where the creative work of the poet begins.
Grief makes demands of us we don't understand. We feel completely submissive in its presence. In being completely without words, we begin to find them.
Mom and dad…
Your warmth lives on in spite of your demise
Memories flourish everywhere in the here and now;
As your echo brushes against my awareness
From an impenetrable distance you will always hold me near.
Grief energises memory so that we begin the work of building a new relationship with the past. Remembrance is a means make new discoveries about what used to be. Memory is a guardian of our presence in the present moment. To remember is to create, invent, and express.
Memory is touch.
Standing on the Other Side of the Threshold and Looking Back
Grief requires us to live in hindsight for a while.
Often, I found that something quite unexpected and innocent would trigger powerful memories of my parents. I suspect that this will always be the case now. And I want it to be that way.
Once, while walking on a cold winter's day, I experienced the intense presence of my mother (but did not actually see her) as sunlight danced in particles of floating ice crystals that seemed to appear out of nowhere. Perhaps it was just subconscious wishful thinking. I choose to believe otherwise.
Grief walks with us, even on a cold winter day.
In the midst of my grief, I did not merely recall past events, I constantly re-lived them. Over and over again, I circled around particular experiences. And each time I completed the cycle, my understanding of them had shifted ever so subtly. We never relive a memory in exactly the same way.
In losing my mother and father, the contents of my memory have evolved. The death of my parents changed my relationship to the past. I feel the pain of their absence, but they are not gone.
I remember watching their bodies being placed into body bags. Then on to a gurney, and then wheeled away – forever. This was my last visit with them. I can still hear the sound of the zipper.
I have learned that the ultimate purpose of grief and bereavement is to begin a process of re-imagining what life will become. We do not simple "get over" our grief. While we learn to live with it better, it becomes a constant companion, and indeed a trusted teacher and mentor.
Grief forces us to be authentic. It will not tolerate masks and false appearances. We must be brutally honest when grieving. We must be willing to face the truth, and remain standing.
And now, I must continue the journey without them. I am without them now. It will be ok.
Mom and dad…
Your laughter dances in the midst of my sadness
A perpetual source of gratitude;
And now after your passing
I stand firmly in the midst of your presence.
- The seventh article in this series is On the Loss of My Parents: 7 – Their Legacy for Life


text:
- Research
- Open Access
Tumour necrosis factor-α stimulates HIV-1 replication in single-cycle infection of human term placental villi fragments in a time, viral dose and envelope dependent manner
© Kfutwah et al; licensee BioMed Central Ltd. 2006
- Received: 03 March 2006
- Accepted: 23 June 2006
- Published: 23 June 2006
The placenta plays an important role in the control of in utero HIV-1 mother-to-child transmission (MTCT). Proinflammatory cytokines in the placental environment are particularly implicated in this control. We thus investigated the effect of TNF-α on HIV-1 expression in human placental tissues in vitro.
Human placental chorionic villi fragments were infected with varying doses of luciferase reporter HIV-1 pseudotypes with the R5, X4-Env or the vesicular stomatitis virus protein G (VSV-G). Histocultures were then performed in the presence or absence of recombinant human TNF-α. Luciferase activity was measured at different time points in cell lysates or on whole fragments using ex vivo imaging systems.
A significant increase in viral expression was detected in placental fragments infected with 0.2 ng of p24 antigen/fragment (P = 0.002) of VSV-G pseudotyped HIV-1 in the presence of TNF-α seen after 120 hours of culture. A time independent significant increase of viral expression by TNF-α was observed with higher doses of VSV-G pseudotyped HIV-1. When placental fragments were infected with R5-Env pseudotyped HIV-1, a low level of HIV expression at 168 hours of culture was detected for 3 of the 5 placentas tested, with no statistically significant enhancement by TNF-α. Infection with X4-Env pseudotyped HIV-1 did not lead to any detectable luciferase activity at any time point in the absence or in the presence of TNF-α.
TNF-α in the placental environment increases HIV-1 expression and could facilitate MTCT of HIV-1, particularly in an inflammatory context.
- Luciferase Activity
- Placental Malaria
- Placental Fragment
- Term Placental Tissue
- Placental Chorionic Villus
In utero transmission of the human immunodeficiency virus type 1 (HIV-1) is one of the routes through which infants acquire HIV-1 from their mothers [1]. However, the placenta forms an efficient barrier between maternal and foetal circulations and plays a crucial role in the regulation of mother-to-child transmission (MTCT) of HIV-1. The trophoblasts are the placental cells in direct contact with maternal circulation. It has been observed that transmission of HIV-1 between T lymphocytes and trophoblasts is possible [2, 3] and that cell-to-cell contact between HIV-1 infected peripheral blood mononuclear cells (PBMCs) and cells of trophoblast origin led to the passage of virus from the apical to the basolateral side of a trophoblast barrier reconstituted in vitro [4]. Soluble factors such as RANTES and MIP-lβ decrease viral passage through this placental trophoblast barriers inoculated with HIV-1 infected cells while TNF-α and IL-8 increase it [5].
On the other hand, isolated primary trophoblasts or malignantly transformed human cell lines of trophoblast lineage are highly resistant to infection with cell-free HIV-1 particles. This resistance has been shown to be bypassed when HIV-1 envelope are substituted by the vesicular-stomatitis virus G protein in the HIV-1 pseudotypes used to infect trophoblast cells [6]. Vidricaire and colleagues observed that cell-free viruses are able to enter with high efficiency into trophoblast cell lines [7]. However viral entry is mediated in large part through endosomal vesicles which ends up in the degradation of a majority of these viral particles but the initial presence of HIV-1 within the endosomes seems to be mandatory for infection to take place in these cells [8]. Production of fully infectious HIV-1 particles by trophoblast cells was only seen following treatment with TNF-α or IL-1 and subsequent co-culture with indicator cells bearing a reporter gene. This confirmed a previous study showing that cytokines, notably TNF-α, stimulate replication of HIV-1 in trophoblast cells [9].
Few studies have been carried out on term placental tissue ex vivo [10]. Maury et al. showed that term placental tissue can support a low HIV-1 replication when infected with cell-free laboratory viral strain used at high doses and after coculture with indicator PBMCs. No study to the best of our knowledge addressed the effect of TNF-α on HIV-1 replication within the placental tissue. Since TNF-α is known to be expressed in the placenta environment especially during viral [11, 12] or parasitic infections [13, 14], HIV-1 placental tissue infection was studied in the presence or absence of recombinant human TNF-α using the placental histoculture system recently described [14] and a single-cycle viral replication system.
Placental histocultures replicate VSV-G pseudotyped HIV-1 in a time and viral dose dependent manner in the absence of TNF-α stimulation
As shown on Table 1, only background level of luciferase activity was detected after 48, 96 and 120 h of histoculture when the envelope deficient (delta-Env) pseudotyped HIV-1 was used.
When placental fragments were infected by the VSV-G pseudotyped HIV-1, a dose dependent replication was observed with an increase of luciferase activity overtime. At the lowest dose of VSV-G pseudotyped HIV-1 used (0.2 ng of p24 antigen/placental fragment), after an increase at 96 h, the luciferase activity dropped at 120 h. In contrast with higher doses, the increase remained up to 120 h.
TNF-α significantly increases the replication of VSV-G pseudotyped HIV-1 in placental histocultures
In all experimental conditions, the dose effect of TNF-α (5 vs 50 ng/ml) did not vary with the duration of histoculture (absence of interaction between TNF-α and duration of histoculture) and TNF-α effect was homogeneous across doses. Therefore, the TNF-α effect is presented globally as mean ratio, whatever the TNF-α dose used.
When the envelope deficient (delta-Env) pseudotyped HIV-1 was used, luciferase activity was not modified by TNF-α after 48, 96 and 120 h of histoculture duration (mR of 1.16 ± 0.20, p = 0.45) (Figure 2D).
With the Xenogen system, infection with the VSV-G pseudotyped HIV-1 was also detected, demonstrated by an increase in the bioluminescence emitted from placental fragments stimulated with TNF-α compared to those without TNF-α treatment. In a representative case shown in Figure 4, bioluminescence values without TNF-α were 31,501 photons/sec, and 93,449 photons/sec with 5 ng/ml of TNF-α. Using this system, we observed that not all placental fragments were detectably infected with the VSV-G pseudotyped HIV-1 despite the fact that they all underwent the same treatments. No bioluminescence was detected in the xenogen system when the delta-Env pseudotyped HIV-1 was used with or without TNF-α stimulation.
This study demonstrates the fact that human term placental chorionic villi can be infected and stimulated to replicate HIV-1 when VSV-G pseudotyped HIV-1 is used. When R5-Env pseudotyped HIV-1 is used, a low level of transient infection can be detected for some of the placentas tested. In contrast when X4-Env pseudotyped HIV-1 is used, no infection is detected.
Previous studies have shown that malignantly transformed human cell lines of the trophoblast lineage are resistant to cell-free HIV-1 pseudotypes bearing the R5 and X4 envelopes [6]. In this study we demonstrate that the human placental chorionic villi are not permissive to X4-Env pseudotyped HIV-1 and display limited permissivity to R5-Env pseudotyped HIV-1 as exemplified by the low luciferase activity values observed even in the presence of the highest dose of TNF-α. This lack of activation of the HIV-1 replication with TNF-α contrasts with the results obtained with choriocarcinoma cell lines by Vidricaire and colleagues who reported an increase of luciferase activity in these cells after infection with R5 or X4-Env pseudotyped HIV-1 in the presence of TNF-α [7]. These apparent contradictory findings might be explained by different experimental conditions. Our study was performed on human term placental tissue and not on cell lines. R5 or X4-Env pseudotyped HIV-1 viruses were used in a dose range of 20 to 100 ng of p24 antigen to infect human placental fragments in contrast to the 250 to 400 ng of p24 antigen used by Vidricaire and colleagues to infect cells. Finally in our experiments, the TNF-α treated placental fragments were not co-cultured with indicator cells to amplify replication because we used non productive single-cycle replicating viral pseudotypes.
The fact that 3 placentas out of 5 were modestly and transiently infected by R5-Env pseudotyped HIV-1 might have some biological relevance in the context of HIV-1 MTCT. Indeed, HIV-1 MTCT occurs mainly with R5 viruses. Additionally, 5–10% of infants born to HIV infected mothers are infected in utero in the absence of any prophylaxis [15]. Thus, there might be a difference in the susceptibility of some placentas to cell-free R5 virus infection and transmission.
The chorionic villi are made up of several cell types such as syncytiotrophoblasts, cytotrophoblasts, Hofbauer cells and stromal cells which has been shown to express variable levels of surface CD4, CXCR4 and CCR5 [16–19]. The expression of CD4 on the surface of trophoblasts is still a matter of debate, however even if they express CD4, they do not produce detectable viral particles when infected with fully competent infectious cell-free viruses [6].
A dose dependent infection was observed with VSV-G pseudotyped HIV-1 whose replication is increased by TNF-α. This implies that TNF-α would increase viral replication in the placenta when the initial resistance to cell-free HIV-1 infection in vivo is bypassed, after cell-to-cell infection for example. In fact, proinflammatory cytokines such as TNF-α and IL-8 have been shown to increase viral replication in different cell lines, isolated primary cells or cervical explant tissue [9, 5, 20]. Indeed, in our study, TNF-α appeared to be capable of stimulating HIV-1 replication in term placental chorionic villi with the various cell subpopulations present. It should be recalled here that VSV-G pseudotyped HIV-1 are able to infect any cell type, regardless of receptor and coreceptor surface expression, due to its amphotropic nature. It has been previously described that the mechanisms by which TNF-α enhance HIV-1 replication occurred via the NF-kB pathway [21]. TNF-α activates HIV-1 LTR-driven transcriptional activity in choriocarcinoma cell line [7] and isolated primary trophoblast cells [9]. Such a mechanism might also occur in placental chorionic villi. The fact that the TNF-α stimulation of HIV-1 replication is not dependent of the dose tested (5 and 50 ng/ml) is consistent with a recent finding demonstrating that the temporal profile of the NF-kB activity is invariant to a wide range of TNF-α doses going from 0.01 ng/ml up to 10 ng/ml [22].
It should be noted that the TNF-α effect is time and VSV-G pseudotyped HIV-1 concentration dependent. Hence, at 0.2 ng of p24 antigen of VSV-G pseudotyped HIV-1/fragment, the increase of replication by TNF-α is only observed at 120 h post-infection whatever the cytokine concentration. At 2 ng of p24 antigen of VSV-G pseudotyped HIV-1/fragment, the effect of TNF-α is only significant when data were analyzed globally for all the time points tested whereas at 20 ng of p24 antigen/fragment, the TNF-α effect is barely significant due to an already high basal replication without TNF-α (Table 1).
Hiv-1 pseudotype replication in the human placental histoculture system in the absence of TNF-α stimulation
Luciferase Activity (Relative Light Units/mn): mean (SE), n
Time post-infection:
48 h
96 h
120 h
168 h
216 h
HIV-1/Delta-Env (20 ng*)
2,660 (356), 3
3,300 (1,637), 3
2,830 (911), 6
HIV-1/VSV-G (0.2 ng*)
4,010 (1,224), 3
14,110 (9,591),3
5,420 (4,152), 3
HIV-1/VSV-G (2 ng*)
14,520 (16,718), 3
77,880 (22,016), 3
93,640 (34,095), 7
HIV-1/VSV-G (20 ng*)
213,190 (195,249), 3
1,266,430 (449,346), 3
1,397,280 (861,789), 3
HIV-1/Ba-L (20 ng*)
2,562 (312), 6
2,535 (475), 5
2,670 (286), 4
HIV-1/Ba-L (50 ng*)
3,830 (3,612), 6
10,380 (7,478), 5
2,625 (772), 4
HIV-1/HXB2 (50 ng*)
2,620 (827), 3
3,010 (135), 3
2,210 (399), 3
HIV-1/HXB2 (100 ng*)
2,950 (458), 3
2,860 (255), 3
2,580 (1,046), 3
Altogether, these results highlight the subtle equilibrium between the viral load, the timing of cytokine expression and the regulation of viral replication within the placenta that might ultimately influence HIV-1 MTCT.
The demonstration that TNF-α could enhance HIV-1 replication in the placental tissue is important, since a shift in cytokine production towards a proinflammatory profile (notably TNF-α) has been associated with Plasmodium falciparum placental infections. [23, 24, 13]. This is of particular public health concern in regions of the world where HIV-1 and malaria are both endemic. Malaria has been shown to reach peak transmission periods during the raining seasons [25]. In an earlier work, a peak in HIV-1 MTCT was reported three months after the peaks of the rains in Cameroon [26], suggesting that the observed increase in HIV-1 MTCT could have been due to an increase of TNF-α in the placental environment as a result of malaria. The present study showing an increase of HIV-1 replication by TNF-α within the placental tissue supports this hypothesis. However direct evidence are still required before we can conclude that placental malaria is linked with an increase transmission of HIV-1 from mother-to-child via an increase of placental proinflammatory cytokines. As discussed in a recent review, several published studies show no, little or significant association of malaria with HIV-1 MTCT [27]. Some studies done on cohorts of pregnant women found no correlation between placental malaria and in utero HIV-1 MTCT [28]. Others studies have demonstrated that the effects of malaria on HIV-1 MTCT depends on placental malaria parasitemia [29].
In summary, data presented here show, by using a single-cycle replication system, that TNF-α enhances HIV-1 replication in chorionic villi of the human placental tissue once viral entry is overcome, as seen with the VSV-G pseudotyped HIV-1. This implies that in vivo TNF-α would increase viral replication in the placenta when the initial resistance to cell-free HIV-1 infection is bypassed, after cell-to-cell infection for example. Altogether, these results highlight the subtle equilibrium within the placental environment between the viral load, the timing of proinflammatory cytokine expression and the regulation of viral replication that might ultimately influence HIV-1 MTCT.
Placenta collection and preparation
Term placentas from HIV-1 negative women were collected in accordance with French ethical guidelines after programmed caesarean section at the Antoine Béclère (Clamart, France) and Robert Debré (Paris, France) hospitals. Placentas were processed within 2 to 3 hours following collection to isolate chorionic villi. After extensive washes with normal medium (RPMI-1640 supplemented with 10% heat-inactivated fetal calf serum (FCS), 1% L-Glutamine and 1% Penicillin/Streptomycin), each placenta was cut to obtain several villi blocks of 2 to 3 mm. Blocks were then distributed in 12-well plates, nine villi blocks per well, and in triplicate wells. For each placenta, 63 to 144 blocks were prepared.
Viral pseudotype preparation and infection of placental fragments
Luciferase reporter viruses were prepared as previously described [6], by co-transfecting 293T cells with the NL4-3 luciferase virus backbone (pNL-Luc E-R-) and VSV-G or HIV-1 R5 (Ba-L) or X4 (HXB2) env plasmids, with the aid of SuperFect (Qiagen, France). Each pseudotype preparation was tested for effective infection at different concentrations on HeLa P4P. Briefly, 104 cells/well/100 μl were seeded in 96 flat-bottomed well plates. Infections were performed 24 hours later. Cells were lysed 72 hours post infection with 100 μl of lysis buffer (Promega, Madison, WI, USA). 100 μl of commercially available luciferase substrate (Promega) were added to 20 μl of each lysate and then luciferase activity read with a luminometer (Lumat LB 9501, Berthold) as relative light units (RLU) per minute.
Different concentrations of the various viral pseudotypes in histoculture medium (RPMI 1640 supplemented with 1% Penicillin/Streptomycin, 1% L-Glutamine, 15% FCS, 0.1% gentamycin, 1% Fungizone, 1% non-essential amino acids, 1% sodium pyruvate -all from Gibco BRL Ltd, Paisley, Scotland, UK) were left in contact with the placental fragments in triplicate wells. Each set of triplicate placental wells contained one of the following pseudotype doses: 0.2 ng, 2 ng and 20 ng of p24 antigen/fragment of VSV-G-pseudotyped HIV-1, 20 ng and 50 ng of p24 antigen/fragment of R5-Env pseudotyped HIV-1 (Ba-L), 50 ng and l00 ng of p24 antigen/fragment of X4-Env pseudotyped HIV-1 (HXB2) and 20 ng of p24 antigen of the envelope deficient pseudotype (delta-Env pseudotyped HIV-1) used as a negative control for infection. All contacts were left overnight at 37°C in a 5% CO2 incubator.
Placental histocultures and TNF-α stimulation
After overnight contact, the nine villi blocks from each well were transferred into 70 μm nylon cell strainers (BD Bioscience, Belgium) and rinsed three times in PBS (Gibco BRL Ltd, Paisley, Scotland, UK). As previously described [14], the villi blocks from each set of triplicate wells were then placed on collagen sponge gels (Pharmacia & Upjohn company, Germany) inside three different wells soaked in one case with 3 ml of plain histoculture medium and in the two other wells soaked in 3 ml of histoculture medium containing recombinant human TNF-α (R&D systems, Deutschland) in final concentrations of 5 and 50 ng/ml respectively.
Luciferase activity quantification by luminometer or by ex vivoimaging system
Histocultures in the presence or absence of recombinant human TNF-α were stopped after 48, 96, 120, 168 or 216 hours post-infection. All nine fragments on each sponge were pooled inside a 14 ml round bottom tube containing 500 μl of lysis buffer (Promega, Madison USA) and homogenised with a homogeniser (Ultraturax, X620 CAT M-Zipperer GmbH, Germany). 100 μl of lysed tissue supernatants was mixed with 100 μl of commercially available substrate (Dual-Luciferase Reporter Assay System, Promega, Madison, USA) and then luciferase activity read with a luminometer (Lumat LB 9501, Berthold) as relative light units (RLU) per minute. For each type of pseudotype (VSV-G, Ba-L, HXB2 or negative control), the different doses of pseudotype and 3 to 4 different durations of histoculture were studied on the same placenta, all without and with the two doses of TNF-α.
We adapted a complementary ex vivo imaging system, (Xenogen IVIS100 and data analyzed by the Living Image Software, Xenogen Corp., Alameda, CA, USA), to visualize and measure the effect of TNF-α on HIV-1 replication in the placenta. Placental fragments were left overnight in contact with 2 ng of p24 antigen/fragment of VSV-G pseudotyped HIV-1 or 20 ng of p24 antigen/fragment of delta-Env pseudotyped HIV-1, then placed on collagen sponges with 0 or 5 ng/ml of TNF-α for 120 hours. The fragments were then collected and placed in contact with 1 μM luciferine (Promega) for 4 hours at 37°C, then the bioluminescence of placental fragments was detected in the Xenogen system and the photons per second from a constant region of interest (ROI) were summed.
Statistical analysis
For each experimental condition, type and dose of pseudotype and duration of histoculture, luciferase activity was measured without and with 5 or 50 ng/ml of TNF-α on three to seven different placentas from different donors. Results of the luciferase activity without TNF-α stimulation are presented globally as mean ± se. To take into account the fact that the activities without and with stimulation were evaluated on the same placentas, the effect of each dose of pseudotype was expressed as the ratio of the activity with each dose of TNF-α to the activity without TNF-α in the same experimental condition, noted ratio of infection, R. Then, for each type and dose of pseudotype, these ratios were analysed through analysis of variance using a factorial design including 2 factors, TNF-α dose (5 or 50 ng/ml) and duration of histoculture (either 48, 96, 120 hours, or 120, 168, 216 hours), and 3 to 4 replicates of placentas. For the duration of histoculture of 120 and 168 hours, 5 to 7 replicates were available for some type of pseudotype. First, it was tested if TNF-α dose effect varied with the duration of histoculture (interaction between TNF-α dose and duration of histoculture). Second, if no interaction was evidenced, the homogeneity of TNF-α effect across doses was tested independently of the duration of histoculture, whereas, in presence of interaction, the homogeneity of TNF-α effect across doses was tested for each duration of histoculture. Finally, the TNF-α dose effect was tested, either globally if homogeneity was evidenced or for each dose otherwise, by comparing the mean of the corresponding ratios to 1, using the residual error derived from the analysis of variance. The TNF-α dose effect was expressed as the mean of the corresponding ratios (mR) and its standard error (mR ± SE). Therefore a mR of 4.0 for a given dose of TNF-α means that the luciferase activity was multiplied on an average by 4 after stimulation when compared to no stimulation.
We would like to thank Pierre Versmisse for his technical help.
This work was supported by the Centre Pasteur du Cameroun, Institut Pasteur and INSERM, Paris.
AKWK has a fellowship from the French Ministry of Cooperation, SCAC, Yaoundé, Cameroon and AA from SIDACTION, France.
Authors' Affiliations
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