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Today's cuppa: Barry's Irish breakfast tea, chased with decaffeinated Irish breakfast tea (all the Irish, half the caf)
In case you've been in an Antarctic weather station for the last few months, there's a presidential campaign going on, and the presumptive nominees are Illinois Sen. Barack Obama for the Democrats
(that should become official during the Democratic National
Convention, which starts on Aug. 25) and Arizona Sen. John McCain for the Republicans (that should become official during the Republican National Convention, which starts on Sept. 1).
Next week, cable newser Fox News premieres two one-hour specials on the personal and professional lives of each of the candidates, called "2008 Presidential Character and Conduct," airing Monday and Tuesday, Aug. 18 and 19, both at 8 p.m. Eastern.
Bill Hemmer is anchor of the Monday special, which focuses on Sen. Obama.
Eric Shawn is anchor of the Tuesday special, which focuses on Sen. McCain.
But this isn't the last opportunity for voters to watch news specials that help them get to know the candidates.
Back during the Television Critics Association Press Tour in July, I spoke to CNN correspondent John King (click here
to see that post, which includes audio
clips) and discussed two
specials that cable newser is doing, one on each candidate, scheduled to
coordinate with the two conventions.
On Tuesday, Oct. 14, PBS airs "Frontline: The Choice" (click here
for the official site), which profiles both candidates. During TCA,
producer Michael Kirk appeared as part of
a "Frontline" press-conference panel, YouTube video excerpts from which are posted here, here and
After watching the videos, you may wonder why
there's so much conversation about former Bush advisor Karl
Rove during the panel — including Kirk's open invitation to Rove to sit down for an extended interview. It's because this panel took place the day
before Rove appeared at Press Tour as part of a panel for Fox News,
for which he works as a political analyst.
Don't know if Kirk will get that interview, since Rove said he has a book coming out in the fall of 2009, quipping, "If you pre-order on Amazon.com, you can get the personalized nameplate."
I also posted a blog entry about that day.


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- Research article
- Open Access
Two chitinase-like proteins abundantly accumulated in latex of mulberry show insecticidal activity
© Kitajima et al; licensee BioMed Central Ltd. 2010
- Received: 28 July 2009
- Accepted: 28 January 2010
- Published: 28 January 2010
Plant latex is the cytoplasm of highly specialized cells known as laticifers, and is thought to have a critical role in defense against herbivorous insects. Proteins abundantly accumulated in latex might therefore be involved in the defense system.
We purified latex abundant protein a and b (LA-a and LA-b) from mulberry (Morus sp.) and analyzed their properties. LA-a and LA-b have molecular masses of approximately 50 and 46 kDa, respectively, and are abundant in the soluble fraction of latex. Western blotting analysis suggested that they share sequence similarity with each other. The sequences of LA-a and LA-b, as determined by Edman degradation, showed chitin-binding domains of plant chitinases at the N termini. These proteins showed small but significant chitinase and chitosanase activities. Lectin RCA120 indicated that, unlike common plant chitinases, LA-a and LA-b are glycosylated. LA-a and LA-b showed insecticidal activities when fed to larvae of the model insect Drosophila melanogaster.
Our results suggest that the two LA proteins have a crucial role in defense against herbivorous insects, possibly by hydrolyzing their chitin.
- Chitinase
- Herbivorous Insect
- Insecticidal Activity
- Chitinase Activity
- GlcN
Laticifer cells of plants are unique in shape, development and function. These cells form long tubular or branched structures that run through the plant body, and large amounts of cytoplasm are exuded when the plant body is cut. The cytoplasm is a sticky white fluid called latex, and in the case of the para rubber tree (Hevea brasiliensis) and rubber fig (Ficus elastica), insoluble latex particles are a well-known commercial source of natural rubber [1]. Laticifers are not present in all plant species. For example, well-studied model plants such as Arabidopsis, rice, wheat, and tobacco, do not have laticifers. However, they have been reported in about 12 500 plant species in 22 families, including monocots and dicots, and are estimated to exist in up to 20 000 species in 40 families [1].
One important feature of laticifers is that they contain various toxic compounds in the latex; for example, the neurotransmitter dopamine in the Persian poppy (Papaver bracteatum), narcotic alkaloid morphine in the opium poppy (Papaver somniferum), and insecticidal compounds such as the glycosidase inhibitors 1,4-dideoxy-1,4-imino-d-arabinitol (d-AB1) and 1-deoxynojirimycin (DNJ) in mulberry [2, 3]. In addition, cysteine protease in latex of papaya (Carica papaya) and wild fig (Ficus virgatalatex) is toxic to caterpillars of herbivorous insects [4]. The compounds are often highly condensed in the latex; for example, d-AB1, DNJ and its related compound 1,4-dideoxy-1,4-imino-D-ribitol are present at 8-18% dry weight in latex of mulberry leaf; 100 times higher than that in the whole leaf [5]. Rubber is also toxic to herbivorous insects because its stickiness limits movement of their mouthparts [6].
These findings suggest that laticifers are specialized cells that have a role in defending plants against herbivorous insects. The distribution of laticifers is associated with vascular tissue, which transports metabolites, water, minerals and signaling molecules through the plant body, and is one of the most important plant tissues. Laticifers might protect the vascular system. Long tubular or branched network structures are effective in this role, because they enable laticifers to exude large amounts of latex at damaged sites. In the present study, we speculate that proteins that are abundant in latex have a defensive role against herbivorous insects. Here, we report that two proteins that are abundant in the soluble fraction of mulberry latex have insecticidal activities against the model insect Drosophila melanogaster, possibly through their chitinase activity.
Purification of LA-a and LA-b
Amino acid sequence analysis
Analysis of glycosylation
Analysis of chitinase and chitosanase activities
LA-a and LA-b also were able to hydrolyze chitosan, which is partly deacetylated and therefore solubilized chitin. Chitosanase activity was tested at a pH range of 3-7, and maximum activity was observed at pH 5 (Figure 5B). At pH 5, the chitosanase activity of LA-a and LA-b was 1.8 and 1.4 times higher, respectively, than that of hen egg lysozyme (data not shown). In the report by Mayer et al. [9], chitosanase activity of 2 chitinase isoforms was 30 and 100 times higher than that of hen egg lysozyme, and the activity of 4 isoforms was 1.7-3.5 times higher. One isoform showed no chitosanase activity.
These results indicated that LA-a and LA-b had chitinase and chitosanase activities, although the activities were relatively low compared to those of orange chitinases.
Insecticidal activity against larvae of D. melanogaster
In this study, we showed that the two proteins LA-a and LA-b were abundant in the soluble fraction of mulberry latex, and had low, but significant chitinase and chitosanase activities. In addition, both LA proteins showed insecticidal activity against larvae of D. melanogaster. The finding that these major proteins have insecticidal activity supports the idea that laticifers are specialized cells for defense against herbivorous insects.
In a recently published study [7] on mulberry latex protein MLX56, it has been shown that MLX56 is glycosylated and has growth-inhibiting (not insecticidal) activity on the lepidopteran caterpillars of cabbage armyworm (Mamestra brassicae) and Eri silkworm (Samia richini), but not on mulberry specialist silkworm (Bombyx mori). Similarities between LA-a and LA-b proteins and MLX56, with respect to N-terminal amino acid sequence and electrophoretic mobility in SDS-PAGE, suggest that they are the same or similar proteins. However, contrary to our results, MLX56 has been reported to have no chitinase activity [7]. Enzyme activity of MLX56 may need to be analyzed using various substrates. In our study, we observed chitinase activity of LA-a and LA-b when using CM-chitin-RBV and chitosanase activity when using chitosan (Figure 5), but the activity was hardly detectable when 4-methylumberryferyl β-D-N, N', N"-triacetylchitotriose (4-MU-(GlcNAc)3), a common substrate for plant chitinase assay, was used (data not shown). Substrate specificity of LA proteins and MLX56 could be different from that of common plant chitinases.
Chitin, a substrate of chitinase, forms the cuticle of the exoskeleton in insects, and should be the target of LA-a and LA-b in the larvae of D. melanogaster and other insects. In addition to external body surfaces, the inside of the mouthpart is also a possible target of LA-a and LA-b because the pH inside the mouthpart is expected to be near that of crude extract of mulberry tissues (weekly acidic or neutral) and to be optimal for the chitinase activity of LA proteins.
Plant chitinase is well known to have antifungal activity, but only a few studies have investigated its toxicity to insects. It has been reported that transgenic tomato that accumulated poplar (Populas sp.) chitinase inhibited development of Colorado potato beetle (Leptinotarsa decemlineata) larvae, and the amount of chitinase in the leaves was >0.3% (w/w) [10]. Two papers have reported that insect chitinase showed toxicity to insects; chitinase of tobacco hornworm (Manduca sexta) included at 2% in an artificial diet showed 100% mortality of larvae of merchant grain beetles (Oryzaephilis mercator) [11], and transgenic tobacco leaves that accumulated chitinase of tobacco hornworm at 0.02-0.03% of total leaf protein inhibited growth of tobacco budworm (Heliothis virescens) larvae [12].
The insecticidal activity of LA proteins might not be caused simply by chitinase activity. It could be that a domain of LA proteins with unidentified biochemical activity cooperates with the chitinase domain to enhance insecticidal activity. Estimated molecular masses of LA-a and LA-b (50 and 46 kDa) were higher than those of common plant chitinases (20-40 kDa). This is at least partly caused by glycosylation, but it also suggests that the proteins might have another functional domain and biochemical function, working in cooperation with chitinase activity for efficient toxicity. Further analysis is needed to understand the molecular mechanism of insecticidal activity of LA-a and LA-b.
Unlike some other insects that eat leaves of latex-producing plants, silkworm eats mulberry leaves without removing the latex by cutting or trenching veins (see [13, 14] for reviews). Therefore, silkworms probably have a mechanism for detoxifying the insecticidal activity of LA proteins, and are unsuitable for studying potential insecticidal activity of LA proteins. One of the reasons why we used D. melanogaster in this study is that this insect does not eat mulberry and thus is not expected to overcome potential toxicity of LA proteins. It would be interesting to know to which species of insect other than D. melanogaster (e.g. pest insects of economically important corps) the LA proteins are toxic. Some insect species including silkworm would be tolerant to LA proteins, and it would be interesting to investigate the mechanism by which they can overcome the toxicity of the LA proteins.
In the present study, we indicated that two chitinase-like proteins (LA-a and LA-b) were abundantly accumulated in latex of mulberry and suggested that they have a crucial role in defense against herbivorous insects, possibly by hydrolyzing their chitin.
Plant material
Mulberry (Morus alba L. cv. Minamisakari) was maintained at the Center for Bioresource Field Science, Kyoto Institute of Technology.
Purification of LA proteins
Latex exuded from the cut petiole of mulberry was mixed rapidly with an equal volume of buffer A (100 mM potassium phosphate, 10 mM EDTA, pH 6.7) supplemented with 0.1% (v/v) β-mercaptoethanol, frozen in liquid nitrogen, and stored at -80°C. Six milliliters of the latex/buffer A mixture was diluted 4 times with buffer A and centrifuged at 18 000 g for 30 min. The supernatant was fractionated by ammonium sulfate between 30 and 80% saturation. Precipitated protein was dissolved in 4 ml of buffer B (10 mM potassium phosphate, 1 mM EDTA, pH 6.0) and dialyzed against the same buffer overnight, using a dialysis membrane with a molecular weight cut-off of 25 kDa (Spectra/Por 7; Spectrum Laboratories Inc., Rancho Dominguez, CA, USA). The sample was diluted to 50 ml and loaded onto a CM-Cellulofine C-200 (Seikagaku Kogyo KK, Tokyo, Japan) cation-exchange column (26 mm i.d. × 100 mm) equilibrated with buffer B. Protein fractions that contained LA-a and LA-b were eluted with buffer B that contained 0.2 M KCl at a flow rate of 1.0 ml min-1. Eluted protein solution was brought to 30% saturation with ammonium sulfate, and hydrophobic interaction chromatography was carried out on a HiLoad 16/10 phenyl sepharose HP column (16 mm i.d. × 100 mm; GE healthcare, Piscataway, NJ, USA) equilibrated with buffer B that contained ammonium sulfate at 30% saturation. The column was developed with a linear gradient of 30-0% saturation of ammonium sulfate at a flow rate of 2 ml min-1. Fractions that contained LA-b were desalted twice by gel filtration chromatography using PD10 (GE healthcare) and 10DG (Bio-Rad, Hercules, CA, USA) column, and further purified on a UNOS1 cation-exchange column (Bio-Rad) eluted with a linear gradient of 0-0.5 M NaCl in buffer B at a flow rate of 3.0 ml min-1. Purified LA-a and LA-b proteins were concentrated with Microcon YM-10 (Millipore Corp., Bedford, MA, USA) to 1.0 mg ml-1, frozen with liquid nitrogen, and stored at -80°C. All purification steps were performed at 4°C. All chromatography steps were carried out using a BioLogic Duo-Flow chromatography system (Bio-Rad). Protein concentrations were determined according to the equation: protein concentration (mg ml-1) = 1.45 A280 - 0.74 A260 [15].
Western blotting
A rat polyclonal antibody was raised against purified LA-a by a standard procedure. For western blotting, protein samples were separated by SDS-PAGE and electroblotted onto polyvinylidene difluoride (PVDF) membrane using the buffer system of Kyhse-Anderson [16]. The membrane was reacted with the polyclonal anti-LA-a antibody, followed by alkaline phosphatase-conjugated anti-rat antibody according to Coligan et al. [17]. A mixture of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) (Promega, WI, USA) was used for color development.
Edman degradation
Cysteine residues of proteins were alkylated by treatment with iodoacetamide according to Coligan et al. [17]. Proteins were then purified on a C18 reversed-phase column (4.6 × 150 mm; TSK gel ODS-100S; Toso, Tokyo, Japan) eluted with a linear gradient of 5-50% acetonitrile in 0.1% trifluoroacetic acid at a flow rate of 1.0 ml min-1. Eluted proteins were concentrated by evaporation and analyzed by automated protein sequencer PPSQ-21A (Shimadzu, Japan).
Analysis of glycosylation
Protein samples were separated by SDS-PAGE and the gel was stained, first with Pro-Q Emerald 300 to detect glycosylated proteins, and then with SYPRO Ruby stain (both from Invitrogen, Carlsbad, CA, USA) for total proteins, according to the manufacturer's instructions. As a control, CandyCane glycoprotein molecular weight standards (Invitrogen) were used. For the lectin blot, protein samples were separated by SDS-PAGE and electroblotted onto a PVDF membrane as described above. The membrane was reacted with biotinylated RCA120 (Vector Laboratories, Burlingame, CA, USA), and subsequently with alkaline phosphatase-conjugated streptavidin. A mixture of BCIP and NBT was used for color development. Fifteen other lectins (ConA, DBA, LCA, PHA-E4, PNA, RCA120, UEQ-I, WGA, ABA, DSA, Lotus, MAM, PHA-L4, SBA, and SSA) were purchased from Seikagaku Kogyo (Tokyo, Japan).
Chitinase and chitosanase assay
Colorimetric chitinase assay was performed using CM-chitin-RBV (Loewe Biochemica, München, Germany) as a substrate, according to Mayer et al. [9], except that the enzyme reaction was prolonged from 10 min to 2 h. Buffers used were 0.1 M citrate-Na2HPO4 buffers (pH 5-8). Hydrolyzed chitin was recovered and measured by absorbance at 550 nm. Activity was expressed as Δ550 nm g protein-1 min-1. Fluorometric chitosanase assay was performed using soluble chitosan (Chitosan, low molecular weight; Sigma-Aldrich, MO, USA) as a substrate according to Osswald et al. [18], except that the enzyme reaction was prolonged from 30 min to 2 h. Buffers used were 0.1 M citrate-Na2HPO4 buffers (pH 3-7). Controls consisted of the complete reaction mixtures stopped immediatedly after addition of enzyme, as described by Osswald et al. [18]. The reaction product glucosamine (GlcN) was recovered, labeled with fluorescamine (Fluka, Buchs, Switzerland) and monitored fluorometrically (excitation: 395 nm; emission: 493 nm). Chitosanase activity was calculated on the basis of GlcN equivalents using a GlcN standard curve generated under the same conditions as these used for the assays.
Insecticidal assay
Drosophila melanogaster (Canton-S strain) was reared at 25°C on standard yeast agar medium. To test insecticidal activity of LA-a and LA-b proteins, 15 2-day-old larvae (second instar) were placed in a vial that contained 200 mg Formula 4-24 instant Drosophila medium (Carolina Biological Supply, Burlington NC, USA), rehydrated with 0.8 ml PBS supplemented with 1 mg LA protein and 10 mg Bacto Yeast Extract (Difco, Detroit, MI, USA) as a nitrogen source. This accounted for 0.1% (w/w) LA protein. For control experiments, the diet was supplemented with the same amount of bovine serum albumin (BSA) or no protein instead of LA protein. The vials were incubated at 25°C.
Authors' Affiliations
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- Osswald WF, McDonald RE, Niedz RP, Shapiro JP, Mayer RT: Quantitative fluorometric analysis of plant and microbial chitosanases. Anal Biochem. 1992, 204: 40-46. 10.1016/0003-2697(92)90136-U.PubMedView ArticleGoogle Scholar
- Van Damme EJ, Charels D, Roy S, Tierens K, Barre A, Martins JC, Rougé P, Van Leuven F, Does M, Peumans WJ: A gene encoding a hevein-like protein from elderberry fruits is homologous to PR-4 and class V chitinase genes. Plant Physiol. 1999, 119: 1547-1556. 10.1104/pp.119.4.1547.PubMedPubMed CentralView ArticleGoogle Scholar
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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

subset: commoncrawl


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EXCLUSIVE! AnnaLynne McCord: 'Twilight Drives Me Wild!'
If you want to know what makes AnnaLynne all hot and bothered, here's your answer: a certain teen vampire flick that a certain ex-boyfriend starred in!
Whoa, we're glad to hear that AnnaLynne McCord, doesn't harbor any resentment against Kellan Lutz! The two have been off and on for practically ever. AnnaLynne even managed to confuse HollywoodLife.com when she was spotted with another guy and then told us she was single!
When we caught up with the sexy 90210 starlet at Muscle Milk's Learn to Ride event in LA Dec. 16, she gave HollywoodLife.com the shortlist of what drives her wild. While Kellan himself didn't feature in her top five, his signature movie, Twilight, did!
What else turns AnnaLynne on? "Five things that make me wild are candy, race car driving and fast cars, Bob Dylan, Twilightand Marlon Brando," she told us exclusively.
It looks like AnnaLynne goes for older guys too! Peter Facinelli is taken, AnnaLynne, but if you're looking for an old vampire, we hear the Volturi are still available!
The 22-year-old actress also confided that she's up for a new movie role, but was cagey as to what film she might be appearing in because she didn't want to jinx it! Hmm….could Anna be angling for a role The Twilight Saga: Breaking Dawn?
Get More AnnaLynne:


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Halliburton: Hassi Messaoud, Algeria Bulk Cement Plant Modifications
MIINC International, Was selected to repipe and automate one of Halliburton's existing bulk cement plants in Algeria .
Halliburton: Basra, Iraq, Bulk Cement Plant
This project was modeled and prefabricated in MIINC, L.P.'s Dallas Texas facility. It was then containerized and shipped from the Houston ship channel to the port at Basra Iraq. The prefabrication and planning resulted in a record setting site installation of a Bulk Cement Plant for Halliburton.
Exxon-Mobile: Basra Iraq, Oil Field Pipe Line Modifactons
Kurita of America contracted MIINC L.P., a premier oil and gas contractor, to model and construct the process piping and pipe support system for Shintech's Phase II Wastewater Treatment Facility. This multi-system process piping project included erection of catwalks and structural bridge supports to integrate the process piping, process controls and electrical needs of this critical facility.


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26 September 2015
The Best Possible Future
What do you consider to be the best possible future for humanity? And how are you helping it to happen?
Themes: Humanity
16 September 2015
The Business of Politics
Is democracy compatible with a business model?
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08 September 2015
Choosing Governments
How do you decide which policies matter most when choosing governments in elections?
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|gene product
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The biochemical material, either RNA or protein, resulting from expression of a gene. The amount of gene product is used to measure how active a gene is, abnormal amounts can be correlated with disease-causing alleles.
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I often make nutritional recommendations to my patients, usually with the intent of improving their microbiome and lowering their inflammation levels to reduce pain as examples. One of the most convenient sources of omega-3 fats that help to accomplish this goal is the lowly sardine. Now the sardine is very controversial, I realize, but it's a small fish so it is low in environmental pollutants such as mercury, and it comes in a convenient single-serving container that needs no refrigeration. Yesterday I got a glimpse into why some of my patients don't follow my sardine recommendation. A woman in her late twenties confessed to not eating sardines, interrupted by fits of laughter on her part, because…of a cartoon! She thought all along that sardines had their heads on and eyes intact, because that is how she remembered them from the movie "Cloudy with a Chance of Meatballs". Here is a clip from that scene: https://www.youtube.com/watch?v=cSKhRVH1Iv4. Ah, the risks of making meal choices based on cartoons…We both got a good laugh from the discussion! So, FYI, sardines do NOT have their heads and eyes when they are canned! Sometimes a patient's crazy stories can provide a doctor with a welcome break from a busy day. It was greatly appreciated yesterday.
Monthly Archives: June 2014
What your Doctor Isn't Telling You about Antibiotics
Antibiotic resistance is a growing threat in the world. The concept is simple enough: the frequent use of antibiotics causes bacteria to develop resistance. Antibiotic resistance is easily shared among bacteria, potentially resulting in pathogenic bacteria becoming drug resistant. Why is that such a big deal? Because then if you get an infection antibiotics will have no effect! Even a mild pathogen can become fatal through this mechanism.
Many people think that it is only those in the natural/alternative health community who are opposed to the frequent use of antibiotics. This is simply not true! The CDC (Centers for Disease Control) and WHO (World Health Organization) are both advising patients and their doctors to not use antibiotics except in specific cases. That doesn't seem to be what is really happening however, as my patients report that their family doctors put them on antibiotics for almost everything including all of the conditions the CDC warns not to use antibiotics for.
If that weren't bad enough, antibiotics are used a great deal on farm animals, resulting in antibiotic exposure even if you don't get any prescription antibiotics. Antibiotic resistant "superbugs" are now being found in food destined for human consumption, an ominous indicator of the spread of resistance.
There are now "nightmare superbugs" affecting the elderly and frail in healthcare settings, completely resistant to the strongest antibiotics available. This has the potential of becoming an epidemic, yet most people (and most of their doctors) remain either ignorant or skeptical of the facts.
What can you do to protect yourself and limit your contribution to a potential plague? First, learn the CDC guidelines and remind your doctor about them. Secondly, learn how to keep your microbiome (your internal population of beneficial bacteria) healthy and functioning. Learn how to keep your immune system protecting you-in other words, work at being healthy! Understanding how antibacterial soaps and disinfectants may promote these problems (which is very counter-intuitive) is also a good idea.
Please take a moment and read some of the articles I've linked below:
Autism, Gut Bacteria and the HPA Axis-What is the connection?
The HPA axis is not a part of the body that is often discussed. It is a functional "axis" that is used to describe the relationship between three parts of the body: the Hypothalamus, the Pituitary gland, and the Adrenal glands. All three of these organs have critical functions with far-reaching implications for physical and mental health. Many psychiatric drugs have been found to affect the HPA axis, resulting in the therapeutic benefit of the drug. Imbalances in HPA function have been implicated in a wide array of neuropsychiatric conditions including in autism. The gut microbiome, gut bacteria, exert control over the development and function of the endocrine hormone system, in particular the HPA axis. Why does this matter? Because imbalances in gut bacteria can therefore result in imbalances in HPA axis development in early life-and this imbalance has the potential to make the person develop autism (as well as other problems in different individuals). It is important because the gut bacteria are so vulnerable to birth practices (c-section vs. natural), antibiotic use, antibiotics in food, pesticides, herbicides such as RoundUp, and even stress levels perceived by the individual. Higher stress is harmful to the gut bacteria through alterations of the digestive functions, secondary to autonomic nervous system imbalance (more sympathetic, or "fight-or-flight", function). Many of these are factors under our influence if not control! Gut bacterial populations are one of the most variable factors in human health, and yet one of the most neglected. My work on The Symbiont Factor is my contribution to spreading knowledge about the gut microbiome, so that more people can take control of their health and more conditions like autism can hopefully be prevented or successfully treated. The book is being configured/edited/reconfigured/formatted so that it works well on all Kindle download platforms, a task that is keeping me quite busy the last two weeks! Almost there, almost there…It will be so exciting when it is finally published! The book will also be available as a print format following its release as an e-book. Until then, stay tuned in and take care of your gut bacteria!
And, best of all, a slide show from one of the head researchers in the field, Ted Dinan: http://www.genome.gov/Multimedia/Slides/HumanMicrobiomeScience2013/33_Dinan.pdf


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Liberty Tax Service in Chicago, IL
Liberty Tax Service (Income Tax Services) is a financial advisory firm in Chicago, Illinois. This financial advisor is listed on Financial Advisory Firms under Financial Advisory Services - Income Tax Services.
You can reach us on phone number (773) 227-2565, fax number or email address . Our office is located on 1214 N Ashland Ave, Chicago, IL,
For full contact details to this financial advisor have a look at the the column to your right (or scroll if you're viewing this on a mobile device.)


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You show up for the team. You show up for the fellow fans. You show up for your people and wear your team colors with pride. We're the guys who meet you at your car. We'll haul your stuff. We'll bring the coolers and the ice. We're the guys with the TV, who are happy to set it up. We're the guys who stick around after the game to clean up. Let us know what you need, and we're the guys who make it happen.
We are Tailgate Guys. We come to serve.
We work carefully with each of our partners to create tailgating locations that offer our guests the ideal game day space.


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Stay one step ahead with Shutterstock's Creative Trends infographic, their look at the styles and trends that will dominate 2019. With information gathered from…Read More
Stay one step ahead with Shutterstock's Creative Trends infographic, their look at the styles and trends that will dominate 2019. With information gathered from…Read More
Selling your photos on the global stock photography market is a great way to get your photography out there, and for people to use…Read More
Color theory analyses how different hues and shades interact with one another. The first step in developing your own color palet is to decide…Read More
If there is one online article any stock photographer should read each year, it is Shutterstock's annual trend report. Packed with the latest trend…Read More
Shutterstock's annual creative trends report has become one of the biggest highlights for photographers wanting to stay up to date on current trends. And…Read More
At first glance, the terminology used in the stock photography industry can be difficult to understand. This is a easy guide for anyone to…Read More
This week Shutterstock launched it's new Editor for customers in beta version. A fast and simple way to edit stock photos. Shutterstock Editor benefits…Read More
Pantone unveiled their 'color of the year' for 2016. A great help when needing to select color palettes for your ultimate stock photos, Taking into…Read More
Improve the sale of your stock photos by implementing four important types of composition techniques to achieve visual balance in your photographs. Christina Wang,…Read More
Shutterstock's data team has identified the fastest growing colors over the past year by matching pixel data with image download behavior from their customers, including…Read More


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Thursday, July 12, 2018
VIDEO: Strongman – Monster ft. B4Bonah
Sarkcess Music act, rapper Strongman comes through with the official visuals to his previously released single titled "Monster". It features fast rising talent B4Bonah, with production credits for the trap song going to Tubhani. The video was directed by Jeneral Jay. Check it out below
Labels: MOVIE & TV, VIDEO
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Let me just come out(no pun intended) and say that I'm not a huge Mariah Carey fan, so I would never consider myself a "lamb" as her fans are affectionately called. But when one of her lambs sent me the video of an onstage gay wedding proposal that took place Saturday night at the Palms Hotel during Mariah's recent Las Vegas concert... I couldn't help but gush a little bit over the diva.
The adorable couple is Maurie Sherman and Mathew Almeida of Toronto, Canada. Maurie tells the LA Times about the months of preparation that went into the two minute wedding proposal that has now been viewed over 50, 000 times on YouTube.
From The LA Times:
According to Sherman, he spent six months working to arrange what you see in the clip, and he bombarded everyone from the Palms to Perez Hilton, until he finally got the ear of Mariah Carey's management company. And, even then, nothing was for sure:
"I worked as hard as I can to make it happen. Everyone loved the idea. But no one made promises. I did not find out until five minutes before the concert started. Her security came over and talked to me. 'I think we are going to do this. And, I think it will be during the show on stage.' I did not know before that moment. But I came prepared. I dressed nicely and brought the candy ring in my pocket and made sure it didn't break. Mathew had no idea.
Unfortunately, Maurie & Matthew will never get to experience the cheap thrill that most heterosexuals experience by having a quick drive-thru Elvis Presley Chapel wedding in Las Vegas because gay marriage is illegal in Nevada. But luckily for them they live in Canada where marriage equality is the law of the land.
Congrats to the happy couple!
Thanks Automatic Prince


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ABM Says Postal Settlement Means Rates will Increase on June 30
Wednesday, January 09, 2002
Press release from the issuing companyWhen the financial impact of the September 11 attack and anthrax were added to the economic downturn, it became obvious that the increased postal rates contained in the Postal Service's rate filing in September would turn out to be inadequate. Mailer's were then confronted with a Hobson's choice. If we did not agree to a rate settlement that would allow for an increase on June 30, rather than in September as would be the case absent a settlement, the Postal Service would revise its rate request by significantly increasing the requested rates. Nearly all of the parties to the Postal Rate Commission proceeding have selected the earlier effective date/smaller increase option as the lesser of two evils, but not before the other publishing interests in the case and the Postal Service agreed to a modification proposed by American Business Media that would retain the flat editorial pound rate. By January 18, parties that oppose the settlement must so advise the Postal Rate Commission, which will then determine by the end of the month whether the opposition (if any) is sufficiently limited to permit a timely Recommended Decision on the settlement proposal. We expect that it will, and that a rate increase averaging approximately 11.5 percent for American Business Media member publications will take effect on June 30. Certainly, American Business Media members should plan for that result, although they should also recognize that the rates will contain greater incentives for palletizing and, especially, for drop-shipping pallets and that many may be able to cut the increase by taking advantage of these incentives.
Industry News
Copyright © 2019 WhatTheyThink. All Rights Reserved


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Google Adsense is Best way to get income online now days many web bloggers and website owner make money with adsense.
First Question First Why Choose Google Adsense for Making Money Online
Its has largest number of publisher 1 million Wow!, you can get paid any country, Google Adsense can be the best income resource for blog owners though it takes really serious efforts in drawing high figures of site visitors. The basic concept of online marketing is just the same with getting offline small business in which clients have to be familiar with brand names and they will maintain using the goods or services when they are pleasant. When a website or a blog can get large traffic, then there will be greater chances for visitors to click the ads by Google Adsense. The number of clicks can Estimate the amount of income that website or blog owners will get and make money with Google Adsense.
How to Become an Adsense Publisher Make Money
All you need a Domain its going to cost you around 10$ you can find a lot cheaper than that, look for offers and coupons online and a Hosting now wait, you are thinking what is this hosting?, what does it use for well just like your cell phone SIM its require a phone to get connected the calls to others in the same way its host our site keep our data online, I recommend you should use it on WordPress platform which I am using right now There are many domains and hosting providers choose any and make sure you check their reviews that's matters a lot in the online world.
Write articles on a particular topic you're interested in, such as fashion, computer, mobile, etc Always remember Google Adsense don't like such keywords "downloading free xyz software" and adult stuff so avoid wasting your valuable time.Always think about your views provide them the information they are seeking for use Keyword Planner for make post on niche you are into.
- Go to Google/Adsense enter your gmail account and click continue.
- Put your website name, click continue
- Fill your address and payee name,phone number
- login in adsense after 4 hours after getting halfly approve google will review your blog it need to done by placing the code in the blog
- Wait for some days you will get the ones you get full approval "Your Google AdSense application has been fully approved. Within a few hours you'll begin to see live ads"
Happy Earnings Hope you make good money with Google Adsense.


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NBC's David Gregory Falls Silent In The Face Of Debunked Benghazi Myths
Blog ››› ››› OLIVIA KITTEL
NBC's David Gregory pointed a series of questions about Hillary Clinton's role in the 2012 Benghazi attacks to Senator Rand Paul (R-KY), allowing Paul to attack Clinton with the long-debunked smear that she was aware of the need for additional security forces at the Benghazi compound yet denied the requests.
On the June 22 edition of NBC's Meet The Press, host David Gregory posed a series of questions on Hillary Clinton's role in the 2012 attacks on diplomatic facilities in Benghazi, Libya, but failed to correct Sen. Paul's false smears that Clinton refused requested security. While discussing the possibility of Clinton running for president in 2016, Gregory asked Sen. Paul about whether "the prosecution of foreign policy," is "the main argument" against Clinton's candidacy. In his response, Paul invoked the debunked myth that Hillary Clinton refused "multiple requests for more security" in the months leading up the attacks.
Later in the interview, Gregory asked whether Benghazi is "disqualifying" for Clinton's potential 2016 candidacy, again allowing Sen. Paul to claim that Clinton "was not responsive to multiple requests for more security." Paul concluded that the American people "want a commander in chief that will send reinforcements, that will defend the country, and that will provide the adequate security," implying for a third time that Clinton refused security she knew was necessary to the Benghazi compound:
But Sen. Paul's smear is based on an old, discredited right-wing media attempt to blame Hillary Clinton for the deaths of U.S. personnel in the Benghazi attacks that originated with an April 2013 Republican Report. The report claimed that an April 2012 cable, sent under Clinton's name, was a "critical cable" that called for reductions in security in response to to the U.S. ambassador's request for additional security resources. The report's evidence that the cable came from Clinton was that it bore her signature, though as Media Matters noted at the time, "several news outlets reported that it is routine for outgoing messages from the State Department to be sent under the secretary's name without the secretary's direct involvement."
Sen. Paul's claims have been discredited for quite some time -- Clinton's signature was standard for every cable, and does not indicate her direct involvement in the cable. As Foreign Policy magazine pointed out:
It's not clear who in the State Department sent the April 19 response. But as a general rule, "every single cable sent from Washington to the field is sent over the secretary of state's name," a former State Department official noted, adding, "Though they are trying to make this new, it's not. After 30+ hearings and briefings, thousands of pages, this has all been addressed."
In September, House Democrats corrected the Republican report, explaining, "The Committee has now obtained the cable referenced by Chairman Issa, and it includes a pro forma line with former Secretary Clinton's name, similar to millions of other cables sent from the State Department."
Washington Post fact-checker Glenn Kessler, who spent nine years covering the State Department, wrote that "every cable from an embassy bears the 'signature' of the ambassador -- and every cable from Washington bears the 'signature' of the secretary of state," concluding that "Issa has no basis or evidence to show that Clinton had anything to do with this cable -- any more than she personally approved a cable on proper e-mail etiquette. The odds are extremely long that Clinton ever saw or approved this memo." Additionally, in an interview with Media Matters, a former 27-year foreign service officer and Accountability Review Board member corrected the claims, saying, "Every single cable going out is signed 'Clinton,' it is the normal procedure... Millions of cables come into the operation center every year, not thousands, millions. And they are all addressed Hillary Clinton."
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Before I get into this, let me throw out definitions, just to ensure that we're all on the same page.
- a thing that blocks one's way or prevents or hinders progress.
- a fence or other obstacle that prevents movement or access.
An obstacle hinders of prevents progress, a barrier simply prevents. Let me suggest that there are very few real barriers in life, rather, there are many obstacles and choices that go with them.
For example, I had returned to college as a working adult to complete my bachelor's degree. I got pregnant. Pregnancy was an obstcle to completeing my degree, but I chose to stop attending school because for me personally, working, parenting, and going to school was too much. Another person could have made an entirely different choice and still have been right, just like I made the right choice. But let me be clear, pregnancy was not a barrier, it did not prevent me from completing my degree, it just made the path trickier.
But I find that there are very few real barriers in life, very few things which actually prevent a person from doing something that is truly a priorty for them. I fall into this camp:
I am reluctant to consider a closed door a barrier.
Yet many people would follow this path:
(except shouldn't they just accept it instead of excepting it? Or is this to suggest that you omit it, I guess that works, but still, c'mon man).
Let me give an example. In the movie, "The Lion King", Simba watches his father die, and considers this a "closed door". He goes off, finds new friends, and lives a "Hakuna Matata" life, which is great. But when he finds out that he's needed in his old community, he reexamines the situation, and decides to bang down that closed door. While his life with Timon and Pumbaa is cute and fun, you simply don't have a hero until Simba decides that his closed door is just an obstacle, and obstacles can be overcome.
The formula applies in every story that has ever held conflict in which a character becomes a hero. While I admit that stories are fiction, the same applies in real life. Imagine if Walt Disney had taken his his failures as closed doors and decided to become a used car salesman. Or Stephen King? (A personal favorite of mine). Or JK Rowling? The list goes on.
I don't know about you, but for me, I'm not taking that closed door as a sign that I should just move on. I am a person with discernment and will, and as such, with each closed door, I will decide how to proceed. This doesn't mean that I won't walk away from some closed doors, but it does mean that I will never assume a closed door is not meant to be opened, beaten down, or otherwise circumvented.


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As well as breaking the Tab down, these videos give left and right hand fingerings. To listen to the Combined Arrangement Guitar Tab again, go to the Combined Arrangement Tab video. Want to revise the melody for Cry me a River? Go to the Guitar Lesson Melody section. Alternatively, why not go back and revise your chords in the Guitar Lesson Chords section.
You are here: Home / Guitar Song Lessons / "Cry me a River" Guitar Lesson / Combined Arrangement / Cry me a River Combined Arrangement: In detail Videos


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Did you ever read Hermann Hesse ? No ? Please do ! You will find much beauty and wisdom, you may even find your life and mindset transformed. Favorite books include 'Siddhartha', 'Narcis and Gulmund' ("Sol og Måne" in Danish) and the 'Glass Bead Game'. Here comes a few qoutes :
Happiness is a how; not a what. A talent, not an object.
It is not our purpose to become each other; it is to recognize each other, to learn to see the other and honor him for what he is.
There's no reality except the one contained within us. That's why so many people live an unreal life. They take images outside them for reality and never allow the world within them to assert itself.
My take is that most of us have 'forgotten' some of these truths (if we subscribe to them, anyways), sadly. But it is never too late to start the quest for hinsight, wisdom and love. Let me finish this post with a poem by Hesse as well.
I can give you nothing
that has not already
its origins within yourself
I can throw open no
picture gallery
but your own
I can help make
your own world visible-
that is all.


text: Five Tips for Your Perfect Wedding Makeup 01/01/2016 Every bride knows just how much goes into planning their dream wedding – from finalizing details with your photographer and florist, to finding the perfect dress; having the right venue and choosing your dinner entrees, and making sure all of your guests have a place to stay – the last thing on your mind is what shade of lipstick you'll wear! Makeup tends to be one of those last-minute details that slip by the wayside, yet it has a tremendous impact on your wedding day. Having the right makeup


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U.S. Adds 157,000 Jobs; Unemployment Rate Unchanged at 7.9%
The Labor Department said U.S. employment increased by 157,000 in January, and the unemployment rate was essentially unchanged at 7.9%. Retail trade, construction, healthcare and wholesale trade added jobs over the month.
In January, employment in construction increased by 28,000. Nearly all of the job growth occurred in specialty trade contractors (+26,000), with the gain about equally split between residential and nonresidential specialty trade contractors. Since reaching a low in January 2011, construction employment has grown by 296,000, with one-third of the gain occurring in the last four months. However, the January 2013 level of construction employment remained about 2 million below its previous peak level in April 2006.
Manufacturing employment was essentially unchanged in January and has changed little, on net, since July 2012.
To read the full Labor Department news release click here.


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Pristine condition.
Signed by author's great granddaughter on front flyleaf. Dust jacket shows mild edge wear.
Minor wear. Stamped markings blacked out at top edge.
Book itself is Like New, with tips-only wear at cover outer corners, 1/8" or less wear at top/bottom spine. Board edges & faces clean, show little if any wear. Silver gilt print very bright both spine & front cover. Page edges and faces clean, and just lightly tanned (and light foxing, 1st/last pages & inside covers, around edges); corners crisp. DJ has little physical wear, worst is cosmetic: Several dirty spots on front (3 are 1/4"x 1/2"-3/4", rest less than 1/4" down to dots only); spine darkened (no impact on print), along with about 1/4" band on all cover sides except bottom. Back to physical wear, 1/8" wear at outer corners, top/bottom spine & top front edge; bottom edges, even less than that on back, front has light bending up to 1/2" onto face; back top, 1/4" wear. DJ back face otherwise clean; both faces show little wear.


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I have been so bad about signing on and updating.
This week promises to be BUSY. I have an appointment with the orthopedic surgeon on Thursday morning to find out what he thinks about my hip/back pain. Then a training session at work all afternoon. Then on Friday, foot surgery and I'll be off work for a week recuperating. Oh and on Thursday, I turn 48 *gasp*
I'm looking forward to having the foot surgery…I've been having problems with my toe nails for years and won't miss them at all. It's going to be painful, having NINE of them removed at once (I had one removed a few years back and remember how much that hurt). I'm stockpiling books to read for the week I'll be home. Just think, next summer I can wear sandals and not have to worry about covering my toes anymore!
Just finished dinner…my own creation: raspberry chicken. Browned chicken breasts in a touch of canola oil, then popped them into a casserole dish, covered with some raspberry jam, onions and chicken broth. Yum!
We're making plans for Halloween already. We've invited the neighbors over for a spaghetti dinner and then the daughter and our son will watch movies. We're getting TWILIGHT and JURASSIC PARK. I'm going to watch with them, love that TWILIGHT and am looking forward to NEW MOON! I'll be going to the midnight show when it arrives!
Anyway, that's life in the Burke household.
~ Patty


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Search the Community
Showing results for tags '3'.
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These were fun to paint and super simple since I already had done the priest in metal already so I didn't hem and haw to much about were I was going with the paint scheme. The mythos pack was much better than I thought it would be and I should have picked it up in the original kickstarter. At least I picked it up on the reaper site last month when it showed up for what half a day before it sold out again? lol.


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Texas trooper officially fired over Sandra Bland confrontation, arrest
Brian Encinia's dismissal comes three months after his bosses first announced they had started "termination proceedings."
"Texas Department of Public Safety (DPS) Director Steven McCraw is upholding his preliminary decision to terminate Trooper Brian Encinia from the department for reasons related to the trooper's traffic stop of Sandra Bland on July 10, 2015," the agency said in a statement.
While still awaiting trial on the perjury charges, the trooper may appeal his firing to the Texas Public Safety Commission (PSC), which is the five-member oversight board for the DPS.
Encinia was accused of lying about his arrest of Bland and the traffic stop, which was caught on his dashboard camera.
Bland was driving in Waller County, Texas when Encinia pulled her over for making an incorrect lane change, he told her. The trooper intended to give her written warning, but claimed she became uncooperative and argumentative, which grand jurors later determined to be untrue.
However, the footage of the arrest revealed an increasingly confrontational encounter between Bland and Encinia, with the trooper waving a stun gun and threatening the activist. In the video, he was heard yelling, "I will light you up!"
Encinia arrested Bland and took her to jail. The 28-year-old black woman was found dead in her jail cell three days after her arrest, sparking a nationwide debate and drawing the attention of the Black Lives Matter movement.
BREAKING No indictments in jail death case of Sandra Bland – reports https://t.co/6zeDMuul3gpic.twitter.com/OzfmnrbAgd— RT (@RT_com) December 22, 2015
Bland's death was ruled a suicide. Her family said that they were trying to collect about $500 for her bail when they learned of her death.
They then filed a wrongful death lawsuit in federal court against Encinia, DPS, Waller County and county jailers Elsa Magnus and Oscar Prudente. The trial is set to begin in January 2017.


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Mariah Carey: 'American Idol' was the 'worst experience of my life'
Mariah Carey is throwing shade at American Idol. During an Australian radio interview, when asked if MiMi will appear on the show for its final season, Mariah said, "Hell no, absolutely not. That was the worst experience of my life. I'm not going to get into what it was. But let's just say, I don't think they had any interest in us to have a good experience through that show. Putting two females against each other wasn't cool. It should have been about the contestants instead of about some non-existent feud that turned into even more ridiculousness."
The singer added, "I would never want to be involved in it again, but everyone else can like it."
Read more HERE
Photo: Instagram


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The Chalk Pit
A Ruth Galloway MysteryeBook - 2017
Praise for Elly Griffiths' Ruth Galloway series
"Gripping." --Louise Penny | "Highly atmospheric." -- New York Times Book Review
"Remarkable, delightful." --Associated Press | "Must-reads." --Deborah Crombie
"Wonderfully rich . . . A great series." -- Guardian | "Smart, down-to-earth, and completely believable." -- Mercury News
Far below Norwich is a maze of old mining tunnels. When Ruth Galloway is called to examine a set of human remains in one of them, she notices the bones are almost translucent, a sign they were boiled soon after death. Once more, she's at the helm of a murder investigation.
Meanwhile, DCI Nelson is looking for a homeless woman who he hears has gone "underground." Could she have disappeared into the labyrinth? And if so, is she connected to the body Ruth found? As Ruth and Nelson investigate the tunnels, they hear rumors of secret societies, cannibalism, and ritual killings. And when a dead body is found with a map of what seems to be the full maze, they realize their hunt for the killer has only just begun--and that more bodies may be underfoot.
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Not up to par in the nine book Ruth Galloway series. Plot rambled along o.k.until surprisingly unrealistic resolution. Ruth's skills were hardly needed. Her late, sudden tryst - only the third so far - with policeman Nelson was an uncalled for surprise further tainting the story. Minor character, homeless person Barbara became an out-of-nowhere unnecessary hero. At least the regulars were predictably fun making the book still worth reading.
An exciting story! I will definitely read another novel from this author.
The Ruth Galloway series continues to be one of my favorite. Like mystery writer Deborah Crombie, Elly Griffiths keeps adding more characters, and many have backstories -- strongly urge new readers to start with first title (2009) The Crossing Places.
This is one of my favourite murder-mystery series, and this I think this is the best book of the series so far. Central to the story this time is the plight of the homeless which was depicted well and thoughtfully. I enjoy archaeology and murder mystery and this series has both. All of the main characters are interesting and have been well developed throughout.
The last sentence leaves one wanting to read the next installment...
For a Ruth Galloway mystery, Ruth plays a minor role in this mystery. It's more a Detective Nelson story. The mystery plot also plays a minor role to the relationships among the characters in this novel. I skipped pages of those descriptions. The mystery is solved close to the end of the novel so read the first few pages to get info on the done deed and then skip towards the end where the mystery is solved, if like me, you are not into the odd personal relationships of the characters in this novel/series. The author uses the first person to write the story - another unusual characteristic - one I don't really like. Probably am not enticed to read more by Emily Griffiths if this novel is characteristic of her plotting, writing style, and character development.
A fine entry in the Ruth Galloway series. As usual, I wanted to find out what all of the characters were up to, the mystery was compelling, and the bits of historical info were interesting.
Don't you just love it and hate it when you get a new release book and on the last page want to scream because you need the next book NOW and it isn't even written yet? Start with the first book, but remember that each book is much better written than the last. These characters really grow on you!
This was the most engaging Ruth Galloway mystery yet. So many threads were introduced including the experience of living rough (or being homeless), gender issues in the workplace, broken marriages and affairs, motherhood, religion, the theater, etc.
Ruth is called in to examine bones found underground while Nelson is told about a missing woman who lived on the streets and the plot thickens. Is there truly an underground society or is it just a utopian dream? Why are the murders and disappearances growing in number? All is brought to a most satisfying conclusion by the end of the epilogue with room for future growth in the series.
I would recommend this book to mystery lovers, especially those with an interest in archaeology and/or police procedure.
In her 9th title in the Ruth Galloway series, Elly Griffiths really shines. The plot and writing are sharp and all the details are tied in together so well. I love this series and can't wait until the next one comes out! When several homeless people, known as rough sleepers, are murdered and several women go missing, it's time to investigate the tunnels under the city of Norwich. Ruth and Nelson--I've got my fingers crossed that it will work out for them! Time will tell....
Summer has officially arrived with its annual treat of a new Elly Griffiths novel. Forensic archaeologist Ruth Galloway is back in the ninth book of this intriguing series. Once again, in The Chalk Pit, Elly Griffiths has delivered a hit with her mix of archaeology, police procedural and updates to the personal lives of her unique and interesting characters.
As always with the books in this series, the plot is swift paced and enriched by an historical setting; in this case the old, subterranean chalk mining tunnels under the city of Norwich, England. A contemporary social issue, the plight of the homeless, is also treated in the book with compassion and respect, while disappearing women and several murders contribute to the captivating plot.
To me, the best part of reading a new Ruth Galloway mystery is catching up on the evolving lives of her somewhat unconventional, but always appealing characters. The development of their relationships is as crucial and important to the plot of the book as the crimes they investigate. It is her ability to make each novel fresh and exciting that continues to make Elly Griffiths my favourite mystery writer.
I highly recommend this fascinating series to the lovers of well crafted contemporary mystery novels that have a distinct historical aspect.
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In the world of my parents, and still in the society in which I grew up, reticence was a virtue. Recently, more than once, I have found it necessary to explain why I was not exhibiting more emotion in circumstances where others thought I really should. When, and with whom, should one "share" personal information? Christine Rosen on "The Death of Embarrassment":
...[O]ne young Manhattan resident recently complained in the New York Times, "Everywhere I go, people are fondling each other as if the entire city were a cheap motel room." At work, over-sharing is becoming as vexing an office problem as gossip. Wall Street Journal reporter Elizabeth Bernstein wrote recently of the challenge of erasing from her mind the image of a colleague who, in pursuit of his bicycling hobby, described "shaving his entire body to reduce aerodynamic drag." We have even devised an acronym - TMI, or "Too Much Information" - to capture the uncomfortable experience of listening to people natter on about their personal problems.The Death of Embarrassment — Features — In Character, A Journal of Everyday Virtues by the John Templeton Foundation
What ever happened to embarrassment? Why are an increasing number of us comfortable bringing our private activities - from personal hygiene to intimate conversation - into public view? Bernstein and others place some of the blame on the desensitization wrought by reality television and social networking sites like Facebook, both of which traffic in personal revelation. ....
.... Pier Forni, who founded The Civility Initiative at Johns Hopkins University thirteen years ago, recently told Obit magazine, "We are more and more concerned with our own pursuit of personal goals. As we engage in a mad rush for the attainment of our personal goals, we don't seem to have the time or see the point of slowing down for the purpose of being kind to others." Nor have we yet found the right balance between connecting with others and TMI. So the next time you feel like sharing the details of your upcoming bunion surgery with your coworkers, resist. You will not only avoid potential personal embarrassment, but you might just make one small step toward improving civility for us all. [more]


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Saturday, August 31, 2013
Black Beans and Rice
Okay, I know I've been promising this post for a while, but today is the first day my husband and I have both been home. I work 8a - 5p, and he works 4p - 12 midnight. We both have the weekend off. So, that's the only time I get his phone......maybe I need to invest in a smart phone :-\ We'll see.
Okay, this is not the "traditional" way people eat beans and rice, but there is NO WAY to make beans and rice into food porn. (Does using the work "porn" make this post have adult material?) So, I made a no-quesadilla. :)
Very simple process. Get your favorite tortilla, add a slice of your favorite veggie cheese - I used GoVeggie, the purple one - and grill. EASY. I only used one slice of the cheese, half on each side, but that was plenty for me. If you want more, feel free :)
Doesn't this look amazing? Wish I could have shared with you guys - you would have loved it.
Now, I know I've been kind of slow on posts the past few days, but trust me. There is LOTS of yummy food to come. I checked out the Engine 2 Diet cookbook as well as the Veganomicon book. I have a whole week of yummy food planned - you don't want to miss this!!
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Louis Vuitton Black Taiga Episea Agenda Pre Owned
This item is no longer available
Authenticity code: Louis Vuitton MI1002
Authentic: Pre Owned
The Louis Vuitton Agenda PM in Epi Black that will keep you organized at all times. You will want to take this classy and sophisticated accessory with you everywhere. Makes a perfect complement with the best LV style.
- Exterior: Like new
- Interior: Like new
- Description of Interior: Leather Lined
- Interior Pockets: 4 open pockets
- Color: Black
- Hardware Description: Gold Hardware
- Material: Epi
- Width: 7.3 in.
- Length: 1 in.
- Height: 9.1 in.
Product Code: 2191005
Read more about Louis Vuitton Date Code here.
All photos are of actual item being sold. © 2007-2018 LuxeDH.
This Louis Vuitton® item is guaranteed to be authentic. Louis Vuitton is a registered trademark of Louis Vuitton. LuxeDH is not affiliated with or represents Louis Vuitton in any way or form.


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Plant Rental for Selling Your Property
Don't let the state of your property's garden let your sale down!
Make the outside of your property and garden sparkle with life before the photography and marketing begins.
Remember, the outside of your property must make buyers want to see what is inside.
If the garden or exterior areas of your property need any kind of help, let Rent-A-Garden take care of it for you:
- Outdoor plant rental and exterior styling to maximise your sale price
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Service matters...which is why our delivery and installation service is professional and friendly.
Before & After Gallery FAQs Client Testimonials
Small investment pays off
We used Rent-A-Garden to transform our property in Drummoyne for sale in April 2014 and were pleasantly surprised at the many positive comments received by interested parties throughout the campaign. Jacqueline's eye for detail and understanding of our aesthetic requirements meant that our property was presented in an outstanding light to potential buyers. We achieved a sale price that far exceeded our expectations with the investment in rental plants being a very good decision. We can highly recommend Rent-A-Garden if your outside areas require that 'wow' factor that will secure a fantastic sale price.
Tony and Melina
Drummoyne, April 2014


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Campaign ideas. They come to us in the oddest of places. Driving the car (Siri, take these notes!), getting ready for bed (darn, where's my notepad?) and in the shower (crap, no phone OR notepad!). They could be short-term campaigns or year-long, integrated affairs that make a huge and lasting impact.
No matter what the idea, it's crucial to take an entrepreneurial mindset and fully evaluate your idea's strategy and strengths to get past the first hurdle: pitching it to your boss and team.
For examples of great entrepreneurial pitching, we need look no further than the gutsy contestants on ABC's Shark Tank.
Shank Tank is a reality TV series where aspiring entrepreneurs pitch business ideas to a panel of potential investors, aka the Sharks. Convincing these guys takes a lot, and after watching a few seasons, there are some common themes that dictate success when addressed.
Here are eight lessons from the sharks that will improve your next pitch:
1. Paint the big picture. What does your campaign do that is so great? Is it scalable? Can it meet a certain demand? Will it scale? Can you create it fast enough and then sustain it?
2. Multi-purpose your campaign. Can your idea support more than one objective? There's something to be said for a niche idea, but today's world requires tasks to be done better, in a faster amount of time.
3. Audience is key. Who are you pitching this to? If you're pitching a dog clothing and accessories line to someone who isn't a dog owner, then you're not getting buy-in.
4. Does it add real value? There are some really great campaigns out there. But do they actually add value and make prospects' lives better? If the process is more complicated than the benefit of participating, the likelihood is that it won't be deemed valuable.
5. Overvaluing the idea can crush it. Ideas are like our children. To us, they're fantastic, they're perfect, and they work. The bad part? Not everyone sees them the way we do. We can be biased when it comes to our own work, so ask others first how our ideas fit with the bigger business picture and what they're worth.
6. Will it generate revenue? All business ideas, processes and tactics must address the bottom line somehow. Even if it's top-of-funnel, where's the connection to the money? No one is in business simply for pleasure, because that's not how we make a living. It's a harsh truth that the Sharks don't hesitate to point out.
7. Deliver the idea well. Back to the stakeholders discussion: if you don't believe in your idea, others won't either. Rehearse your pitch until you can address all of the above business points clearly and with confidence. Don't over-complicate or exaggerate.
8. "Ideas aren't always a business." This sentence is said at least once an episode on Shark Tank, where entrepreneurs often arrive with an idea that isn't fully developed or researched enough to warrant investment. Be sure you don't do the same!
(Photo Credit: Flickr Creative Commons – mdalmud)


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Posted by Matthew Tobin Anderson on Apr 21, 2019
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Ga boiler vent damper wiring diagram
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MPG (aka Miles Per Gallon) were an Atlanta based act who formed in late 1978. Bearing a style reminiscent of Toto or New England, the band rose to notable popularity in the southeast before A&M Records beckoned, prompting the release of their eponymous debut in 1981. Led by singer/keyboardist/songwriter, Steve Carey, MPG typified a sound that was in full swing by the early 80's. With excellent label support and regional airplay, it's quite astounding that the album slipped by audiences and fell into total oblivion. This unfortunate setback led A&M to pull the plug early into the band's contract, leaving them minus the three subsequent albums they had signed on to make. It wasn't long before members began to defect and by 1982, the band were inactive. The members have all gone on to pursue careers in and out of the industry, though nothing quite on the scale of MPG.
Sadly, this well done AOR album has been collecting dust at A&M and there appears to be no sign of a reissue anytime soon. There have been talks of a reunion over the years, but nothing has materialized to that end as of yet. In the meantime, while we wait, take a listen to this exceptional album and enjoy a piece of southern AOR the way it's meant to be done.
192kbps @ http://www.megaupload.com/?d=FG5IXFKJ


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By Dan Clayton
Follow @danclayt0n
Whether they like it, or even realize it, the Portland Trail Blazers and Utah Jazz are on parallel paths; and the two clubs will be dealing with each other for years to come. Both were rebuilt on the fly to become bubble playoff teams a year ago (Portland got in, Utah fell just short), and both are widely expected to be a notch better than that this season.
That's why it's interesting whenever these franchises – at similar points on their respective journeys – wind up in the same gym. They started their 2016-2017 seasons squaring off in Portland on Tuesday night, and Damian Lillard's Trail Blazers got the better of this first matchup. Sixteen of Lillard's 39 points came in an unstoppable fourth quarter that saw Portland take and keep the lead on the way to a 113-104 victory.
Lillard attacked switches, challenged Utah's paint protectors and hit tough fadeaways over Jazz defenders, connecting on four of five shots and all six free throws in the game's final eight minutes. Nothing the Jazz did seemed to bother Lillard at all – not even the 9-foot-7 standing reach of Rudy Gobert, whose length was tested (and bested) at least twice.
Nice finish over Gobert by Lillard off of the PnR https://t.co/OlNDt4akHa
— BBALLBREAKDOWN (@bballbreakdown) October 26, 2016
Even before Lillard went off, the game was largely determined by shooting prowess. The Blazers shot 68 percent from three for the game, including a 4-of-5 performance by Allen Crabbe off the bench. His 18 and CJ McCollum's 25 kept the Blazers in striking distance leading up to Lillard time. Noah Vonleh punished some defensive miscommunications by Utah on his way to a 5-for-5 night (including three jumpers), and Al-Farouq Aminu rattled a couple of corner threes in.
The Jazz spent much of the night inviting Portland pull-ups with a defensive scheme that matched their available personnel. Without starter Derrick Favors, the Jazz mostly had bigs hang back while guards followed the likes of Lillard and McCollumn over high screens. This allowed Blazer ball handlers a little pocket to squeeze a shot off, and they obliged. Portland also ran a lot of 4- and 5-out motion stuff and, while the Jazz were diligent about rotating, the home team patiently kept the ball moving until it found an open player.
More often than not, that open player was someone who could can a jumper, and that's really what this game down to. Portland converted 63 percent of its uncontested jumpers per NBA stats, while the Jazz only managed to make 37 percent of theirs. In a game that wasn't out of reach until the final minute, that's a pretty enormous difference.
While a lot went right for Portland, the game also wasn't a resounding validation of their offseason. Their prime free agent get, Evan Turner, finished with just three points on 1-of-7 shooting, although he did facilitate to the tune of five assists. Their other offseason addition, Festus Ezeli, still hasn't practiced because of ongoing knee issues. But the Blazers got enough offensive juice from their incumbents to not have to sweat the lack of production from the new guys.
The Jazz, on the other hand, got encouraging debuts from their newcomers. Joe Johnson dominated during a 27-point second half (he had 29 for the game). Iso Joe went to work against matchups in the mid and low post areas, and also hit three catch-and-shoot threes. Joe spent the preseason on cruise control, so a strong showing for him was a welcome boon for the Jazz, who will be without top scorer Gordon Hayward for at least another couple of weeks.
George Hill's Jazz premier was almost as brilliant, in ways that his 19 point, six assist line undersell quite a bit. While he could have been more effective on catch-and-shoots (1/6 overall on threes), the game just had a different feel every time the former Pacer stepped out to the hardwood. The Jazz offense clicked to the tune of 122.4 points per 100 possessions with Hill on the floor; when he was off, it ground to a pretty lackluster 54.8 – a sign that the injury-riddled Jazz still have some depth issues when they're missing main guys.
Hayward and Favors might be Utah's two best players, and sixth man Alex Burks is an important bench scorer who can get to the lane at will. So it stands to reason that the Jazz would sense a drop-off when their 11th, 12th and 13th mean were promoted to rotation roles in the stead of three of the team's top six or seven guys.
Favors' absence probably hurt most of all. Not only is the Atlanta native really good overall, but he has some positional versatility that gives Utah an option while Rudy Gobert sits. Coach Quin Snyder could only afford to sit Gobert for seven minutes last night, because lineups with any other Jazz center combined for a 28.6 ORtg.
Favors is also a key to Utah's defensive identity, especially late in games. Utah likes to switch pick-and-rolls down the stretch, and Favors' defensive versatility and mobility give them the option to switch everything, one through four. Without Favors, that role was left to Boris Diaw, the one 2016 Jazz acquisition who didn't really shine in his debut. The Blazers frequently targeted Diaw, trying to put him in the pick-and-roll defense so they could catch him in his tracks or force switches onto McCollum or Lillard. More conspicuous were Diaw's shortcomings on the offensive end, where he was 1-for-8 and assistless.
Meanwhile, Rodney Hood was on superb, putting his full repertoire on display in a 26-point performance that included spot jumpers, patient drives and one monster dunk. When Hayward and Burks get healthy, Hood and Johnson will team with that pair to make a pretty deep, dangerous wing rotation for the Jazz.
That's good news for Utah, but also for this budding rivalry, which should only grow in intensity as both teams see their principals get healthy and comfortable. Portland and Utah certainly haven't heard the last from one another.


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Surya Flow / Chakra Flow
Surya Flow is a Vinyasa based flow style integrating ancient Indian practices from Hatha Yoga, as well as new modern influences form the West. The practice centres, grounds and opens the body-mind-spirit and challenges students to leave their comfort zones in order to fully thrive. Each class integrates a wide variety of Sun Salutations, the elements of proper alignment, breath, graceful movement, meditation and a balance between yin and yang: slow and fast, invigorating and calming, strength and flexibility, sound and silence, challenging and nurturing.
Chakra Flow is a Vinyasa based yoga style working specifically with the chakras, either through a class focussed on one chakra, it´s corresponding asanas and breathing exercises and the emotional mind-body connection, or by integrating the seven main chakras into one class that brings the overall energy in the body into flow and unlocks the wisdom of each chakra.
Yoga Therapy Coaching
Private Yoga Therapy addresses the ones that would like to look deeper into and alleviate physical ailments, would like to prepare for a yoga group class or would like to understand the connection between physical manifestations of pain and the underlying believes behind them. Each session is tailored to your needs and predisposition and will give you tools you can continue to work with at home.
Meditation opens the mind for the bigger picture. By sitting still many things can happen. However, as sitting still is sometimes hard for our active minds, I mix several holistic techniques. As an artist, creativity and visualization have always been a great inspirations. The modalities I work with include active and guided meditations, creative visualisation, shamanism, art, breath work and movement. According to the person or group or the theme, it can be either more active or more calm.
Monthly playlists from around the globe. Music to move, to lie in the hammock, to dream, to connect with Self.


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Meet New People: City Cocktail Social
Near North | Website
This event occurred in the past.
Map | Find Parking
Sip cocktails and wine while meeting new people. Entry includes a Chi Town Mule. Drink specials available throughout the evening. $10 Prepay includes 1 comp drink, 4 wines at $6,and vodka mixed drinks at $7 Th


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Nail Pop LLC was created in 2012 by Erin Hart, a nail artist living in Tampa, FL. Starting as a mobile nail salon and offering pop up manicures around town, Nail Pop LLC has expanded to provide DIY nail art products internationally. Erin Hart creates and curates radical nail art design ideas to share with her friends and fellow humans under the guise of Nail Pop LLC.
When will I receive my order?
Orders are shipped USPS first class and usually arrive 5-7 business days after processing. International orders may take 3-4 weeks to arrive.
How do I apply my press on nails?!
How do I apply my water slide decals?!
Having trouble with your decals maintaining transparency?
Your topcoat might be drying before it's able to completely penetrate the decals! Try switching to a thicker top coat or one that isn't fast drying. Sally Hansen Double Duty is my current go to!


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"The International Day of Prayer for Men and Boys is a call for peace; it is a call for bringing hope to the hopeless. It is a call for transforming our spiritually, psychologically and emotionally toxic communities into a loving, nurturing, and spiritually vibrant oasis. It is a call to end the "school-to-prison" pipeline. It is a call to end the rising suicide rate of Men and Boys."
This quotation from the 2015 press release of Ms. Diane Sears (USA Coordinator – International Men's Day) is relevant for today's world. I believe that prayer from a genuine heart can transform the world. Many will agree that we live in a confusing world with changing values, strange behaviours, hollow morals and questionable ethics. There is often a blurred line between the truth and lies or right and wrong. Our role models sometimes disappoint us. We cannot lose faith and believe that our society will become worse or events are unchangeable.
I have many weaknesses but prayer has been a guiding light in my life. On a daily basis, I face temptations but prayer has allowed me to understand the difference between right and wrong. This year, I again want to encourage persons and institutions to observe the International Day of Prayer for Men and Boys.
Here is a prayer that I want to share with readers-
We pray for more positive role models
who will inspire the current and future generations.
Continue praying for the millions of sick persons who cannot afford proper health care.
Pray for the volunteers and medical personnel who assist the terminally ill persons.
Continue praying for the mentally and physically challenged so
they will be treated fairly and with respect.
Let us pray for boys and men who are incarcerated.
Pray that the innocent in prisons will be freed,
pray that the guilty will learn from their mistakes.
We pray for men and boys to be more tolerant and accepting of different cultures and religions.
Continue praying for the many persons who have no access to clean drinking water.
Pray for gender equality, pray for the safety of pregnant mothers
who are carrying the future of humanity.
We must pray for suicidal males in abusive, toxic relationships;
give them strength to leave or seek counselling.
Pray that we will establish healthy relationships,
and promote stable friendships.
We pray for boys and men who are materialistic, selfish and greedy;
let them be aware of the need to change their lives and thinking.
Pray for those who have allowed vices to endanger their lives and others.
We pray for world leaders to understand the need for permanent peace.
We pray for a world that will focus less on trivial issues
and concentrate more on helping and uplifting humanity.
Jerome Teelucksingh, Ph.D. is the Founder of International Men's Day, Chairman of the International Men's Day Coordination Committee, faculty member in the History Department at the University of West Indies, an author, and humanitarian.


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CBS Sports' (s CBS) online coverage of the 2010 NCAA Men's Basketball Tournament is shattering records for 2010 — and the fun has just begun. After just four days of the tournament, the March Madness on Demand (MMOD) online video service has delivered more than 8.7 million hours of live streaming video and audio consumed — up 35 percent over the previous year's tournament. MMOD also reported that more than 6 million unique visitors watched video during the opening rounds of the tournament.
The most watched hour of the tournament on both Thursday and Friday of last week began at 2:00 p.m. ET, with viewers tuning in for 910,258 combined hours of video and audio during those two days. The most-watched game online through the first two days was the double-overtime matchup between Floria and BYU, which accounted for more than 521,000 hours of video and audio streamed. Robert Morris vs. Villanova (462,000 hours) and Siena vs. Purdue (403,000 hours) ranked second and third respectively.
There are still two weeks of March Madness coverage left, kicking off again this weekend with the Sweet 16 round of the tournament running on March 25 and 26 and the Elite Eight matchups on March 27 and 28. The Final Four matchups will be played on April 3, with the National Championship game ending the tournament on April 5.
Typically, however, those rounds don't garner as much interest online as the games during the first two rounds of the tournament, many of which are played during the day, when college basketball fans are at work. As Jason Kint, senior VP and general manager of CBS Sports, told us in a phone interview, viewership is highest online at times when users can't watch the games on TV: When the games start being played in prime time, viewers typically switch from viewing online at work to viewing from the comfort of their living rooms. There were also a number of upsets during the opening rounds, which could also lead many fans to lose interest in the later rounds of the tourney.
Related content on NewTeeVee:
Where to Watch March Madness Online
Related content on GigaOM Pro:
Viacom v. YouTube: All Over But the Shouting


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This is Beatrice's productive mess as she watches along as she is sewing!
Are you envious of her lovely 301?
I get the biggest smiles when you send me photos of your sewing space, including me in your creative space!
I know this is an impromptu Quilt-Cam, the opportunity came along suddenly and I grabbed it, knowing that if you missed it, at least you'd be able to catch in in the archives under the Quilt-Cam tab at the top of the blog at your leisure.
It turns out that Mona was able to finish quilting her second quilt and was on her way home by 3pm or so.
After I returned from a post office run, and found myself home alone for the first time in I don't know how long, and realizing that I'd be home alone tonight due to the Hubster having tennis league – I decided to be spontaneous!
Aren't you glad?
I am. April/May/June are going to be insane months and I'm not sure when we will be able to next fit this in, and I'm missing sewing with you!
So what am I doing tonight?
The Lozenges are coming out to play: