text:
New Research In
Physical Sciences
Social Sciences
Featured Portals
Articles by Topic
Biological Sciences
Featured Portals
Articles by Topic
- Agricultural Sciences
- Anthropology
- Applied Biological Sciences
- Biochemistry
- Biophysics and Computational Biology
- Cell Biology
- Developmental Biology
- Ecology
- Environmental Sciences
- Evolution
- Genetics
- Immunology and Inflammation
- Medical Sciences
- Microbiology
- Neuroscience
- Pharmacology
- Physiology
- Plant Biology
- Population Biology
- Psychological and Cognitive Sciences
- Sustainability Science
- Systems Biology
Local modulation of plus-end transport targets herpesvirus entry and egress in sensory axons
Edited by David W. Tank, Princeton University, Princeton, NJ, and approved September 21, 2004 (received for review June 30, 2004)
The core structures of many viruses move within cells by association with host cytoskeletal motor proteins; however, the mechanisms by which intracellular viral particles are transported toward sites of replication or the cell periphery at distinct stages of infection remain to be understood. The regulation of herpesvirus directional transport in sensory neurons was examined by tracking individual viral capsids within axons at multiple frames per s. After entry into axons, capsids underwent bidirectional and saltatory movement to the cell body independently of endosomes. A comparison of entry transport to a previous analysis of capsid axonal transport during egress revealed that capsid targeting in and out of cells occurs by modulation of plus-end, but not minus-end, motion. Entry transport was unperturbed by the presence of egressing virus from a prior infection, indicating that transport direction is not modulated globally by viral gene expression, but rather directly by a component of the viral particle.
The α-herpesviruses are parasites that reside within the vertebrate nervous system, but are typically restricted to the peripheral nervous system (PNS) of the natural host. Initial infection requires retrograde axonal transport of the herpesvirus capsid to the nuclei of neurons in peripheral ganglia, where latency is established. Reactivation from latency can occur throughout the life of the host, resulting in recurrent disease (e.g., herpes labialis produced by herpes simplex virus type 1 and shingles produced by varicella-zoster virus). Reactivated infection requires anterograde transport from ganglia back to exposed body surfaces, where infection can transmit to new hosts. That the mechanism of viral transport and its directional regulation remain to be discovered has important functional consequences: it allows rapid infection and spread without exposure to circulating immune defenses and plays an important role in determining the outcome of infection. Transsynaptic transport of infection from the PNS into the CNS is a rare but devastating progression of disease that typically results in fatal encephalitis (1–3).
The herpesvirus virion consists of a double-stranded DNA genome encased in an icosahedral capsid shell. The capsid is surrounded by an additional layer of proteins collectively called the "tegument," which is in turn enveloped by a lipid bilayer containing viral membrane proteins. The viral genome is transported within capsids, either being released into the nucleus through nuclear pores during initial infection, or assembled with tegument and envelope into mature virions during final egress from a cell (reviewed in ref. 4).
By tracking individual pseudorabies virus (PRV; an α-herpesvirus of broad host range) capsids tagged with the GFP, we previously analyzed the dynamics of newly replicated capsids during egress in axons of infected sensory neurons (5). The GFP signal enabled tracking of individual ≈125-nm capsids in living cells with a temporal resolution of 1–10 frames per s (fps). Although capsid transport was targeted to the terminal ends of axons as expected, individual capsids moved bidirectionally, sometimes moving processively in the retrograde direction for prolonged periods. These findings stand in contrast to those reported for vaccinia virus where egress occurs by anterograde transport along microtubules without any retrograde motion (6, 7). Therefore, the significance of bidirectional transport during herpesvirus egress was not immediately clear.
Here, we describe the dynamics of herpesvirus capsids as they move from axon terminals to neuronal cell bodies during initial infection. As during egress, capsid transport is bidirectional; however, retrograde motion is now dominant. The dynamics of retrograde motion were statistically indistinguishable from the minor retrograde component previously observed during egress. To address whether this control was achieved by alteration of the global intracellular environment resulting from viral gene expression, we looked at the outcome of a secondary viral infection in previously infected cells. We found that newly infecting viral particles moved in a net retrograde direction toward the nucleus, even if the cell had a previous productive infection. Thus, entering virions seem able to locally control their direction of transport and can modulate the host transport machinery to achieve appropriate targeting. We conclude that capsid targeting to either the cell body or axon terminal is achieved by local regulation of the amount of plus-end-directed microtubule transport.
Materials and Methods
Virus and Cells. The recombinant isolate of PRV, PRV-GS443, expresses GFP fused to the VP26 capsid protein and has been described (5). PRV-GS847 is identical to PRV-GS443 but carries the monomeric red fluorescent protein (mRFP1) ORF in place of the GFP ORF, and is therefore a red-fluorescent analog of the GFP-capsid virus (8). PRV-GS847 was made by recombining the mRFP1 fusion into the pBecker3 infectious clone by RecA-dependent homologous recombination in Escherichia coli by using previously described methods (9). The GFP- and mRFP1-fluorescent capsid viruses were propagated, and titers were determined, on pig kidney epithelial cells (PK15). Titers were equivalent to wild-type virus (≥1 × 108 plaque-forming units/ml). To make a virus deleted for the gD glycoprotein gene (Us6), the infectious clone encoding the GFP-VP26 capsid fusion (pGS443) was recombined with an amplified PCR product of a kanamycin selectable marker, resulting in insertion of the marker in Us6. The primers used for amplification of the kanamycin marker were as follows: 5′-GGGGCCCCAGGTTCCCATACACTCACCTGCCAGCGCCATGTAA GAAGTTCCTATACTTTCTAGAGAATAGGAACTTCCCAGTCACGACGTTGTAAAACG and 5′-GGAAGAAGATGTAGACGCACACGCCCACCAGGAGCG CGCCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGAAACAGCTATGACCATGATTACG. The 5′ end of each primer encodes 40 bp of homology flanking the region of Us6 targeted for deletion, and a 34-bp Flp recognition target (FRT) site is encoded by each primer (underlined sequence). The linear PCR product was recombined into pGS443 by RecA-independent homologous recombination in E. coli strain EL250, and the kanamycin marker was subsequently removed by Flp-mediated recombination of the two FRT sites (10). The resulting infectious clone (pGS791) encodes a stop codon (bolded in the first primer sequence) and a single 34-bp FRT site immediately after the ATG start codon, and deletes codons 2–361 of Us6 (400 codons total). The viral gD glycoprotein is responsible for blocking additional viral entry into an infected cell once viral gene expression is underway (11, 12). Because gD is also essential for production of infectious particles, pGS791 was transfected and propagated on G5 swine kidney cells, which constitutively express the PRV gD glycoprotein (in the absence of gD, PRV can egress from cells but the released viral particles are not infectious) (13).
Neuronal Culture. To allow for a direct comparison of newly acquired entry data with that previously collected during viral egress in axons, sensory neurons were isolated and maintained as described (5). Briefly, sensory neurons were cultured from dorsal root ganglia (DRG) of E8-E10 chick embryos, and were seeded on polyornithine-treated 22-mm-square glass coverslips at ≈100 neurons per coverslip. To allow for axon outgrowth, sensory neurons were cultured for 3–5 days before infection with PRV-GS443.
Infections and Time-Lapse Fluorescent Microscopy. Viral stocks harvested from porcine kidney 15 (PK15) cells were frozen, thawed, and sonicated (model VCX500; Sonics & Materials, Danbury, CT) before infection of sensory neurons. The viral stock was diluted 1:10 in Hepes-buffered media (pH 7.4), and ≈70 μl was used to fill a chamber made of a coverslip sealed above a glass slide by using a 1:1:1 mixture of Vaseline, beeswax, and lanolin. All samples were maintained at 37°C during imaging. With the exception of experiments using PRV-GS847 (see below), samples were imaged with a Zeiss 510 laser-scanning confocal microscope by using a 63 × 1.4 N.A. oil objective and a 488-nm argon laser line. This protocol is based on our previous study of capsid egress in axons, except that virus was added to neurons immediately before imaging (5). Steady-state fluorescent labeling of endosomes was achieved by incubating cells for 8–16 h in media supplemented with 0.2 mg/ml 10,000 molecular weight dextran coupled to tetramethylrhodamine (TMR-dextran; Molecular Probes) (14). Cells were washed three times in media lacking TMR-dextran before infection. Simultaneous imaging of TMR-dextran and GFP-capsids was achieved with a 488/543-nm dual band dichroic mirror, and coexcitation with 543-nm HeNe and 488-nm argon laser lines. A 570-nm beam splitter was used with a 560-nm long pass (LP) filter to collect TMR emissions, and a downstream 490-nm beam splitter was used with a 505- to 530-nm band pass (BP) filter to collect GFP emission, thus allowing simultaneous imaging of both fluorophores on parallel photomultiplier tubes. Imaging of PRV-GS847 (see Fig. 4) was performed with a Nikon TE2000 inverted wide-field fluorescent microscope equipped with a 60 × 1.4 N.A. oil objective, Texas red filter set, and Roper Scientific (Tucson, AZ) Cascade charge-coupled device camera.
Image Analysis. GFP-capsids were tracked in axons by using the isee software package, as described (5). Motion was then parsed into periods of uninterrupted motion in a given direction ("runs"). Statistics were then compiled, keeping plus-end-directed runs and minus-end-directed runs separate. Storage of the GFP-containing viral stocks at –80°C and subjecting stocks to freeze/thaw and sonication before infection resulted in capsids with a broad distribution of reduced emissions. In general, fluorescent emissions were somewhat less variable when viral stocks were stored at –80°C for <1 week, and the emissions from individual GFP-particles were similar to those previously described (data not shown) (5).
Imaging Viral Capsids During Entry. Previously, we used time-lapse fluorescent microscopy to image and track individual GFP-tagged capsids during egress transport from neuronal cell bodies to the distal ends of axons (which typically displayed growth cone morphology) of dissociated sensory neurons (5). In the work presented here, we use identical imaging techniques to track GFP-capsids immediately preceding entry from axon terminals to neuronal cell bodies (Fig. 1). Although similar in approach to the previous egress study, examination of entry was complicated by a reduced number of transport events per infection (owing to the small number of virions that enter cells after infection) and decreased GFP emissions from individual particles. The latter was likely a result of storage of the fluorescent viral stocks at –80° before infection. Capsid motion was tracked in dissociated sensory neurons from dorsal root ganglia of embryonic day 8 (E8)–E10 chick embryos. To confirm transport direction, only axons that could clearly be ascribed to a specific neuron or growth cone were analyzed; axons crossing or bundled with other axons were avoided. Capsids moved predominantly in the retrograde direction (toward the cell body). Entry transport was processive (occasionally capsids moved through the field of view, typically 30–40 μm, without pausing) and saltatory. Capsids also moved in the anterograde direction, although this motion was transient and followed by immediate reversal to retrograde motion. Nevertheless entry transport, like egress transport, was bidirectional (5).
Analysis of Motion During Viral Entry. Individual capsids were tracked through successive frames of time-lapse fluorescent recordings until they migrated out of the image field. Our temporal resolution ranged from 1–10 fps. Recordings were begun within 5 min of virus inoculation onto the cells (the majority of capsid entry transport in axons was observed within the first 30 min of infection). Occasionally, we observed a fluorescent capsid, diffusing in the tissue culture media, to make contact with a growth cone, and subsequently to initiate fast retrograde transport toward the cell body (in one instance, the capsid of a newly bound virion initiated retrograde axonal transport ≈1.5 min after the virion contacted the growth cone; see Movie 2, which is published as supporting information on the PNAS web site). In contrast, we never saw particles initiating infection at axon shafts.
Capsid dynamics were quantitated by analyzing periods of uninterrupted capsid travel in one direction (defined as a capsid run). A run ended when capsid motion paused, reversed direction, or was lost from the field of view. Because of the latter, a fraction of runs were truncated as a consequence of the small imaging area required to achieve optimal spatial resolution while maintaining temporal imaging faster than 1 fps. We analyzed 259 runs in the retrograde direction, the dominant component of entry transport. Retrograde runs had an average velocity of 1.17 ± 0.03 μm/s (SEM) and were well modeled by a Gaussian distribution, indicating that a single class of minus-end-directed motor is likely responsible for capsid transport toward the cell body (Fig. 2). Top speeds for retrograde runs were in excess of 3 μm/s. These velocities were calculated as the average speed over the entire length of the run; instantaneous velocities had a wider distribution (not shown). The average length of retrograde runs was 7.38 ± 0.47 μm (SEM), and the distribution of run lengths was fit by a decaying exponential as expected for processive motion. Runs that did not leave the image field typically ended by a reversal in direction; of the 259 retrograde runs analyzed, 68 reversals were observed. We did not observe prolonged anterograde capsid transport; rather, reversal events were short-lived (lasting ≈0.9 s on average) and always ended in an immediate reversal to retrograde motion. Because the average time span of the anterograde runs was in the range of our temporal resolution, quantitation of anterograde dynamics was inherently less accurate. With this caveat, we measured an average velocity of 0.55 ± 0.05 μm/s (SEM) and run length of 0.40 ± 0.03 μm (SEM). In addition to reversals, we observed 27 runs end in loss of motion, which lasted on average for 1.41 ± 0.38 s (SEM) and had an upper observed limit of 10 s. Unlike motion during egress, these pauses in motion were transient, and permanent stalls were rare (9). We cannot rule out the possibility that many observed pauses in retrograde motion were short-lived reversal events below our temporal or spatial resolution.
Capsids and Endosomes Traffic Independently in Axons. Although transmission electron microscopy studies of herpesviruses infections in sensory neurons support a model of viral entry by fusion at the host cell plasma membrane, endocytosis of virions can result in productive infections in some cell types (15, 16). To determine whether axonal transport of capsids was associated with endosome transport, sensory neurons and nonneuronal cells present in the disassociated neural culture were together infected with the GFP-capsid virus after labeling of the endocytic pathway with a TMR-dextran fluid-phase marker. Although GFP-capsid emissions were frequently observed in endosomes of a sub population of nonneuronal cells [presumably Schwann cells (17)], GFP-capsids never colocalized with the TMR-dextran label in axons (Fig. 3).
Comparison of Entry and Egress Capsid Transport. By analyzing entry and egress capsid transport dynamics by identical methods, a comparison of capsid motion is practical (9). Although the anterograde components of motion during entry and egress transport were distinct, retrograde transport was statistically indistinguishable both in terms of velocity and run length (Table 1).
Although the mechanism by which alteration of anterograde motion is achieved is not immediately clear, modulation of capsid anterograde motion may result from global changes in the cellular environment resulting from viral gene expression (i.e., egress targeting of capsids could be effected by a general reconfiguration of the axonal transport machinery). To examine this possibility, we infected neurons with a GFP-capsid virus and then superinfected these same neurons 13 h later with a mRFP1-capsid virus and examined the entry dynamics of the red-fluorescent capsids in the context of an infected cell. Because herpesvirus infection of cultured DRG sensory neurons results in a block to additional viral entry beginning 2–4 h postinfection (18), we used a gD-null derivative of the GFP-capsid virus for the primary infection [superinfection is not blocked by viruses lacking the gD gene (11, 12)] to make this experiment feasible. Cells undergoing productive infection with the GFP-capsid/gD-null virus were observed to support typical net retrograde transport of newly infected mRFP1-capsid virus (Fig. 4).
The genomes of neurotropic herpesviruses must travel long distances in sensory axons of the peripheral nervous system both to establish life-long infection and to reinfect peripheral tissue upon reactivation to spread between hosts. By an unknown mechanism, this process is effected by targeting axonal transport of viral capsids in either the retrograde (toward sensory ganglia) or anterograde (toward the periphery) direction at distinct phases of the infectious cycle. Inadvertent anterograde targeting of the virus may result in devastating forms of encephalitis (19).
Because endogenous vesicle-bound cargoes move in both the anterograde and retrograde directions in axons (14, 20–24), targeted herpesvirus capsid transport could be explained if capsids were present in host vesicles. For example, some neurotropic viral pathogens, such as poliovirus, enter axons by endocytosis and subsequently traverse the axon in the endosome (25). However, by transmission electron microscopy (TEM), herpesvirus capsids are typically observed directly in the cytosol subsequent to entry into axons (15, 26, 27). Similarly, we see membrane-free capsids in axons in our culture model by TEM (data not shown). Consistent with static TEM images, we find that, by imaging both capsids and the endosomal pathway simultaneously in living cells, retrograde transport of capsids in axons is mediated independently of endosome transport. During egress, progeny capsids have also been reported to transport independently of membranes and viral membrane proteins (28–31). Together, these findings indicate that capsid targeting is modulated by virus-encoded factors rather than by default endocytic and biosynthetic pathways of the neuron. For most virus stocks, a fraction of virions fail to initiate infection for a variety of possible reasons. Therefore, it is important to note that all of the particles tracked in this study were competent to enter axons and were not retained in endosomes. We expect that, despite our inability to determine their subsequent fate, the transport of particles must be an important step in the productive infection of neurons.
By comparative analysis of our studies on the dynamics of entry and egress in sensory axons, we make four principle conclusions. First, permanent loss of capsid motion (stalling) during translocation in axons is observed only during egress. The reason is unclear but may result from egress of viral particles from the cell interior to the outer surface of the axon, either at the axon terminal, or less frequently mid-axon.
Second, axonal transport of capsids is consistently bidirectional. Our examinations of entry and egress both show that reversals of direction are often observed to be instantaneous even at a temporal resolution of 30 fps (data not shown). This observation indicates that capsids associate with a bidirectional transport apparatus. Although many endogenous cargoes undergo coordinated bidirectional motion in association with microtubules, the molecular nature of the putative bidirectional motor complex has yet to be discerned [recently, however, biochemical links between kinesins and dynein/dynactin have been identified (32, 33)]. Although several herpesvirus proteins can bind host motor proteins in vitro and in two-hybrid studies, no virus/host interactions have been identified that are required for capsid transport (34–37).
Third, the kinetics of retrograde motion during entry and egress were statistically identical. The simplest explanation is that a minus-end-directed motor, presumably dynein (38, 39), is active at capsid surfaces during both stages of the infectious cycle. More importantly, the similarity of minus-end transport in both cases suggests that overall capsid transport in both entry and egress is due to the same transport machinery, functioning in much the same way in both instances. In principle, capsid motion could involve completely different processes during entry and egress. For instance, as the outer makeup of the capsid could change during entry versus egress (possibly due to the presence or absence of particular viral tegument proteins), one could imagine that entirely different transport machinery could be recruited to move the capsid in these two cases. The fact that the minus-end motion is the same in both entry and egress suggests that this is not the case.
Fourth, the anterograde component of transport is modulated (Fig. 5). We note that, although the processivity of plus-end motion is clearly reduced during entry transport, the reduction in velocity may manifest from our inability to fully resolve the short anterograde runs characteristic of entry dynamics in the present study. Preliminary observations attained at 30 fps indicate that instantaneous plus-end velocities during entry and egress may be equivalent (data not shown). This finding is consistent with work characterizing motion in other bidirectional transport systems including mitochondria, Xenopus pigment granule melanophores, and Drosophila lipid droplets (40–43). In these systems, there is coordination between the opposite polarity motors, and the direction of net transport is determined by altering the amount of plus-end motion while leaving the minus-end motion constant. Because the same seems true here, virus particles may be recruiting a complete bidirectional transport apparatus similar to one used in those systems. Significantly, in two of the previously described systems, there is a key difference: in the melanophores, transport is controlled by a global signal (cAMP/PKA) whereas, for the lipid droplets, a globally acting directionality factor (Halo) determines the amount of plus-end motion of all lipid droplets in the embryo (44, 45). In contrast, global changes in the infected cell are not responsible for differential capsid motion during egress versus entry: the ability of capsids newly deposited into the axon during superinfection to undergo typical retrograde motion indicates that local changes in capsid structure modulate appropriate targeting. This result may be explained if the virion substructure binds a kinase or phosphatase that modifies associated host motor complexes. Alternatively, a viral tegument protein acts locally at the capsid surface to function analogously to the Halo protein (which presumably localizes to the lipid droplets to alter plus-end motion) (45). These possibilities are not mutually exclusive, as two viral-encoded tegument proteins are serine/threonine kinases (46–49). Regulation of a kinesin motor may allow for dynein activity to become processive (50). Little is currently known regarding the composition of the capsid/tegument structure during intracellular transport, particularly during entry transport, although there is some evidence for transport of tegument proteins with capsids during egress (30, 51).
As studies on pathogens such as Listeria monocytogenes have propelled the understanding of actin polymerization and its regulation, we expect that determining the mechanism of directional transport of herpesvirus capsids will gain insight into the general underlining cell biology of coordinated bidirectional transport. In addition, regulation of capsid targeting in the peripheral nervous system may play an important role in determining the outcome of viral infection and provide insights into the mechanism of herpesvirus encephalitis.
We thank K. Coller and S. Noles for their help with constructing viruses PRV-GS847 and PRV-GS791, respectively, and J. Goodhouse for his expert help with confocal microscopy. Members of the Enquist laboratory provided useful comments and discussion. G.A.S. was supported by a Schweppe Foundation award and, in part, by National Institutes of Health (NIH) Grant 1RO1AI056346. L.P. was supported by NIH Grant 1F32 NS04 2967-01A1. S.P.G. was supported in part by NIH Grant 1RO1GM64624. L.W.E. was supported by NIH National Institute of Neurological Disorders and Stroke Grant 1RO133506.
↵ ¶ To whom correspondence should be addressed. E-mail: lenquist{at}molbio.princeton.edu.
↵ § S.P.G. and L.W.E. contributed equally to this work.
This paper was submitted directly (Track II) to the PNAS office.
Abbreviations: PRV, pseudorabies virus; DRG, dorsal root ganglion; mRFP1, monomeric red fluorescent protein; TMR, tetramethylrhodamine; fps, frames per second.
- Copyright © 2004, The National Academy of Sciences
- ↵
Roizman, B. & Sears, E. (1996) in Fundamental Virology, eds. Fields, B. N., Knipe, D. M. & Howley, P. M. (Lippincott-Raven, Philadelphia), pp. 1043–1107.
- ↵
- ↵
Smith, G. A., Gross, S. P. & Enquist, L. W. (2001) Proc. Natl. Acad. Sci. USA 98 , 3466–3470. pmid:11248101
- ↵
Ward, B. M. & Moss, B. (2001) J. Virol. 75 , 4802–4813. pmid:11312352
Campbell, R. E., Tour, O., Palmer, A. E., Steinbach, P. A., Baird, G. S., Zacharias, D. A. & Tsien, R. Y. (2002) Proc. Natl. Acad. Sci. USA 99 , 7877–7882. pmid:12060735
Smith, G. A. & Enquist, L. W. (2000) Proc. Natl. Acad. Sci. USA 97 , 4873–4878. pmid:10781094
- ↵
Johnson, R. M. & Spear, P. G. (1989) J. Virol. 63 , 819–827. pmid:2536105
Campadelli-Fiume, G., Arsenakis, M., Farabegoli, F. & Roizman, B. (1988) J. Virol. 62 , 159–167. pmid:2824844
Peeters, B., de Wind, N., Hooisma, M., Wagenaar, F., Gielkens, A. & Moormann, R. (1992) J. Virol. 66 , 894–905. pmid:1309919
Hollenbeck, P. J. (1993) J. Cell Biol. 121 , 305–315. pmid:7682217
- ↵
Nicola, A. V., McEvoy, A. M. & Straus, S. E. (2003) J. Virol. 77 , 5324–5332. pmid:12692234
Lycke, E., Kristensson, K., Svennerholm, B., Vahlne, A. & Ziegler, R. (1984) J. Gen. Virol. 65 , 55–64. pmid:6319574
Banfield, B. W., Kaufman, J. D., Randall, J. A. & Pickard, G. E. (2003) J. Virol. 77 , 10106–10112. pmid:12941921
- ↵
Hollenbeck, P. J. & Bray, D. (1987) J. Cell Biol. 105 , 2827–2835. pmid:3693400
Kaether, C., Skehel, P. & Dotti, C. G. (2000) Mol. Biol. Cell 11 , 1213–1224. pmid:10749925
Nakata, T., Terada, S. & Hirokawa, N. (1998) J. Cell Biol. 140 , 659–674. pmid:9456325
Overly, C. C. & Hollenbeck, P. J. (1996) J. Neurosci. 16 , 6056–6064. pmid:8815888
Mueller, S., Cao, X., Welker, R. & Wimmer, E. (2002) J. Biol. Chem. 277 , 7897–7904. pmid:11751937
- ↵
- ↵
Penfold, M. E. T., Armati, P. & Cunningham, A. L. (1994) Proc. Natl. Acad. Sci. USA 91 , 6529–6533. pmid:7517552
Miranda-Saksena, M., Armati, P., Boadle, R. A., Holland, D. J. & Cunningham, A. L. (2000) J. Virol. 74 , 1827–1839. pmid:10644356
Holland, D. J., Miranda-Saksena, M., Boadle, R. A., Armati, P. & Cunningham, A. L. (1999) J. Virol. 73 , 8503–8511. pmid:10482603
Tomishima, M. J. & Enquist, L. W. (2001) J. Cell Biol. 154 , 741–752. pmid:11502759
Deacon, S. W., Serpinskaya, A. S., Vaughan, P. S., Lopez Fanarraga, M., Vernos, I., Vaughan, K. T. & Gelfand, V. I. (2003) J. Cell Biol. 160 , 297–301. pmid:12551954
Ligon, L. A., Tokito, M., Finkelstein, J. M., Grossman, F. E. & Holzbaur, E. L. (2004) J. Biol. Chem. 279 , 19201–19208. pmid:14985359
- ↵
Douglas, M. W., Diefenbach, R. J., Homa, F. L., Miranda-Saksena, M., Rixon, F. J., Vittone, V., Byth, K. & Cunningham, A. L. (2004) J. Biol. Chem. 279 , 28522–28530. pmid:15117959
Diefenbach, R. J., Miranda-Saksena, M., Diefenbach, E., Holland, D. J., Boadle, R. A., Armati, P. J. & Cunningham, A. L. (2002) J. Virol. 76 , 3282–3291. pmid:11884553
Ye, G. J., Vaughan, K. T., Vallee, R. B. & Roizman, B. (2000) J. Virol. 74 , 1355–1363. pmid:10627546
Dohner, K., Wolfstein, A., Prank, U., Echeverri, C., Dujardin, D., Vallee, R. & Sodeik, B. (2002) Mol. Biol. Cell 13 , 2795–2809. pmid:12181347
Sodeik, B., Ebersold, M. W. & Helenius, A. (1997) J. Cell Biol. 136 , 1007–1021. pmid:9060466
- ↵
Gross, S. P., Tuma, M. C., Deacon, S. W., Serpinskaya, A. S., Reilein, A. R. & Gelfand, V. I. (2002) J. Cell Biol. 156 , 855–865. pmid:11864991
Chada, S. R. & Hollenbeck, P. J. (2003) J. Exp. Biol. 206 , 1985–1992. pmid:12756280
Gross, S. P., Welte, M. A., Block, S. M. & Wieschaus, E. F. (2002) J. Cell Biol. 156 , 715–724. pmid:11854311
- ↵
- ↵
Zhang, G., Stevens, R. & Leader, D. P. (1990) J. Gen. Virol. 71 , 1757–1765. pmid:2167929
Coulter, L. J., Moss, H. W., Lang, J. & McGeoch, D. J. (1993) J. Gen. Virol. 74 , 387–395. pmid:8383174
Cunningham, C., Davison, A. J., Dolan, A., Frame, M. C., McGeoch, D. J., Meredith, D. M., Moss, H. W. & Orr, A. C. (1992) J. Gen. Virol. 73 , 303–311. pmid:1311359
Muresan, V., Godek, C. P., Reese, T. S. & Schnapp, B. J. (1996) J. Cell Biol. 135 , 383–397. pmid:8896596
Miranda-Saksena, M., Boadle, R. A., Armati, P. & Cunningham, A. L. (2002) J. Virol. 76 , 9934–9951. pmid:12208970


text:
Archive for June 25th, 2010
"Hard fun!"- Guided Math is a Space to Push Thinking!
Papert (1996) tells the story of an exchange between two kindergarteners while exiting the computer lab. One student was exiting with their class and the other was entering.
"[Upon exiting the child waiting to enter asked] "What was it like?" The friend replied, "It was fun." Then paused and added: "It was really hard." The relation between "fun" and "hard" may need some interpretation. Did this mean "it was fun in spite of being hard" or "it was fun because it was hard"? The teacher who heard the tone of the conversation and knew the children had no doubt. The child meant it was "fun" because it was "hard." Since then I have listened to children with an ear sensitized by this experience and have come to know that the concept of hard fun is widely present in children's thinking (Cited in Andrews & Trafton, 2002)."
This passage provokes me to think about the type of "hard fun" which we engage children in during math class. Hard fun is academically rigorous tasks that engage the whole child. When students are having "hard fun" both their hands and their minds are engaged. When students are having "hard fun" they are doing rich mathematical tasks that push them to reach and learn. When students are having "hard fun" they laugh, they communicate and they struggle through the difficult parts hanging onto all the scaffolds we set up.
Andrews, A. & Trafton, P. (2002) Little Kids-Powerful Problem Solvers. NH: Heinemann
Papert, S. 1996. The Connect Family: Bridging the Digital Generation Gap. Atlanta: Longstreet.Read Full Post | Make a Comment ( None so far )

subset: commoncrawl


text:
Hi, I'm Debbie Nol, and I married a car guy. My 14 years of working at Arie Nol Auto Center has been an incredible journey, building amazing relationships with customers and learning so much from our experienced technicians.
Now I'm launching a blog to share what I've learned about cars and some of my most memorable customer stories. Hopefully you will find my blog both helpful and entertaining!
When I started working here sixteen years ago, our kids were young. As with many small, family-owned business', the kids did a lot of the behind the scenes things. Take out trash, clean floors, stock shelves, etc. As they got older, they still did these things but they also worked in the office and helped with customer service. Then they started college and their own careers. Two of them are in totally different businesses and, as many of you know, our youngest, Cameron, is one of the service advisors in the front office. Just when we were trying to figure out what our next step was to fill the now open position, we found out that Luke was looking for a job. He attended the same high school our kids did and we knew his parents. He started working for us a couple of years ago and left at the beginning of this summer for a full-time position elsewhere. His job description we joking referred to as our 'minion'. He painted walls, cleaned bays, helped me clea ... read more
It's summer time in Michigan! Beaches, barbeques and road trips. There are also a lot of car shows. A LOT of car shows. I have attended my fair share of car shows. This is what happens when you marry a car guy. In the early days of dating, I attended a lot of automotive related events. I asked a lot of questions like, "Why are there mirrors displaying the undercarriage of that car?" as well as, "Why is that guy carrying a car hood?" Turns out there was a race car driver autographing whatever you brought in to the show. Fast forward thirty years later and I now work with my husband in the automotive repair business and my life revolves around cars. I can appreciate a beautiful car and the hum of an engine but I don't have the deep down love of the automobile that many people, including the ones I work with every day, do. Several weeks ago in Northern Michigan, my stepmom and I attended a craft show and were walking to a favorite local restaurant. There wa ... read more
I've always been amazed by the damage that can happen inside an engine compartment or on the suspension of a vehicle. The technicians always let me know when they have some car part carnage for me to take a picture of before they repair it. There are many reasons for visible damage. Age and wear, lack of maintenance, and sometimes just a part that fails before its time. Sometimes damage can happen simply due to another part failing and it becomes a domino effect. The technicians always explain the sequence of events that lead to the damage. I've learned a lot from seeing situations like this and having them explained to me. Automobiles are mechanical. There are a lot of moving parts and sometimes if it is a moving part fails, it causes a lot of damage. At times, even a new part can fail. We are choosy about the parts we use for the repairs and maintenance; however, on occasion even a new part will fail. It isn't just disappointing for the customer but also for t ... read more


text:
How to set up a cloud server to autoscale?
We do not set our cloud servers to autoscale by default as our clients have various use cases and needs. It is up to the client whether or not to activate this functionality.
You can set your cloud server to autoscale from your User Area, under Manage Account and click on the Autoscale button. You will create an autoscaling event in three easy steps by setting the conditions, under which you want your account to automatically scale up and meet unexpected traffic or usage spikes. The event occurs upon reaching the threshold 75% of the available CPU or RAM.
1) Choose how many cores of CPU or GB of RAM should be added when autoscaling happens.
2) As the server will autoscale every time its resource usage reaches the threshold usage, we strongly recommend you to set a Monthly Cap. The monthly cap is the sum of your current resource units and the units that will be added when the autoscaling event happens. Thus you will ensure that the added resource units won't go above the limit you set and your charges won't grow unexpectedly.
3) Set the autoscaled event to autorenew or not. If you believe your resource usage will require you to keep the extra CPU or RAM units longer than one month, we advise you to set the Autorenewal option to ON.
You find this article useful? Click here to learn more about SiteGround web hosting experts and what else we can do for you!


text:
It is our utmost priority to ensure that we offer the best products and services to our valued customers. However, there are some situations when our customers might not be totally satisfied with the work we have done. In these occurrences, we strive to put things right as quickly as possible. Your satisfaction is important to us and we are dedicated to ensuring that you are happy.
This document outlines our complaints policy and procedure, so please read the following. If you have any questions about this policy, please feel free to let us know.
When the work has been completed, please inspect it thoroughly as soon as possible. Hopefully you will find that everything has been done according to our usual high standards, but if you find anything you are not satisfied with please let us know right away.
If you have any complaints or comments, you can contact us and we will be able to deal with any issues as quickly and efficiently as possible.
We are available to be reached on in a number of different ways. Please call us on 0800 955 4 955, write to us at 65 Limerick Gardens, Cranham, Essex, RM14 1HZ. or send us an email at firstname.lastname@example.com.
We are committed to responding as quickly as possible and we promise to reply within 7 days of receiving your complaint. Whenever possible, we will let you know a date at which we will remedy any issues that you have brought to our attention. The sooner you let us know about a complaint, the faster we will be able to fix it.
If we are not able to resolve your complaint using our procedure, we will rely on the help of Ombudsman Services in order to resolve the dispute. This will provide an unbiased expert opinion that will help to resolve the matter.
In the very unlikely situation where we are not able to remedy your complaint, you might want to refer your complaint to the Ombudsman Services. If you wish to do this, please contact us as soon as possible.
This is our complaints policy, please let us know if you have any questions about this or any other aspects of our business.


text:
South Ogden:
(385) 626-0977
(385) 439-0101
About Us
Kim Cassity, DMD, MS
Erik Badger, DDS, MS
Meet Our Staff
Office Tour
Dental Implants
What are Dental Implants?
Single Tooth Implants
Multiple Tooth Implants
All About All-on-4® Treatment Concept
Am I a Candidate for All-on-4® Treatment Concept?
Cleaning Dental Implants
After Dental Implant Surgery
Implant Restoration
Dental Implant FAQs
New Patient Forms
Post-Op Instructions
Referring Office
Contact Us
South Ogden
Top 3 Myths About Bleeding Gums
Posted on 10/15/2018 by Cassity & Legacy Implants and Periodontics
Have you ever noticed that when you brush your teeth, you see some blood in your spit? Bleeding gums happen. Some people panic when they notice this, while others ignore it and hope that it will go away.
Both of these reactions are because of the myths that surround bleeding gums. There are many things that people say about this issue, but there are three myths that everyone should know.
Myth #1 Bleeding Gums are Normal
The idea that it is normal to have occasional bleeding of the gums is something that many people believe. They may believe it because they are afraid that it may indicate a more serious problem and they don't want to think about it. They may attribute it to something they did and think that it won't happen again.
While there are times when bleeding gums are nothing more than an accident. They are also an indication of gum disease. The earlier you treat gum disease the easier it is to reverse the damage. Do not think the bleeding gums are normal and talk to a dental professional today.
Myth #2 Bleeding Gums are Healthy
There are some people who claim the bleeding gums are a sign of healthy teeth and gums. The bleeding is the way that gums restore themselves. This is the wrong way to think. While healthy gums may bleed, it is important to know the cause of the bleeding to make sure it is not a bigger problem. Healthy gums will not bleed without some reason, so find out what the reason is instead of thinking everything is fine.
Myth #3 Avoid Brushing Bleeding Gums
Harsh brushing is one of the causes of bleeding gums. A person that has gum disease is more likely to suffer bleeding gums no matter how harsh their brushing is. Because of this, some people avoid brushing their teeth if they have bleeding gums. This can make the problem worse.
If the bleeding comes from the bacteria and plaque in the teeth and gums, brushing is the best way to remove it. Instead of avoiding brushing when you have bleeding gums, visit our dental professionals to figure out what cause the bleeding and what the best treatment plan is.
Bleeding gums are one of the first signs of gum disease. While they may not always bleed, it is always better to find out if there are any problems starting before they get worse. Don't fall into the traps of the top three myths about bleeding gums.
If you have experienced bleeding gums or have any questions about your oral health, contact the dental professionals and ask them any questions you have.
South Ogden:
(385) 626-0977
(385) 439-0101
Copyright © 2016-2020
Cassity & Legacy Implants and Periodontics
WEO Media (Touchpoint Communications LLC)
. All rights reserved.
Go to Mobile website


text: The Mammoth Hunters book by Auel Article Share SHARE Facebook Twitter The Mammoth Hunters book by Auel Learn about this topic in these articles:discussed in biography In Jean AuelThe Mammoth Hunters (1985) finds Ayla and her Cro-Magnon lover, Jondalar, joining a new clan. In The Plains of Passage (1990), Ayla and Jondalar face hardships as they travel to rejoin his tribe. It was 12 years before Auel completed the next book, The Shelters…Read More


text:
- 20131 Review
Voted Most Helpful Review 2013 Forest River Wildwood DLX 402QBQ View Listings
|Overall Rating
|Overall Quality
|Floor Plan
|Driving / Towing
|Factory Warranty / Support
Lots of room in this camper, great size bedrooms, and more storage then we know what to do with. Large bathroom up front with lots of holding tank room. Back bathroom is a little small but love having the door to get to it from the outside.- Tom, Wisconsin
Did you find this useful?
- 1


text:
Trying to stick to an exercise routine is difficult at the best of times, but even more so when the weather is sweltering, and this week with temperatures soaring to 34C in parts of the UK, many of you may have put your fitness regime on the backburner.
However, these tips on how you can stay fit and healthy even when it's boiling outside will help you maintain your fitness levels.
Avoid midday heat
The early afternoon is the hottest part of the day, so if you do feel like going for a run try to avoid the hours between 11am and 3pm. Otherwise, you may find yourself a victim of the sun, and suffering from burns, dehydration and even heatstroke.
Stay hydrated
Whether you work out indoors or outdoors, it is important to stay hydrated at all times. Bring plenty of water with you and make sure you replace the fluids you are losing, particularly during hardcore cardio work.
According to Dr Cedric Cryant, fitness expert for the American Council on Exercise, speaking to Men's Fitness, it is a good idea to drink up to 680ml of water two hours before training in hot weather and another 170ml to 230ml during every 20 minutes of exercise.
Change your regime
If your training schedule sees you doing HIIT (High Intensity Interval Training) or long-distance running over the next few days, it is a good idea to switch it up while it's hot. Perhaps opt for exercise you can do indoors under a fan and away from the sunshine.
Or, you could use this time to concentrate on muscle training, stretching with some yoga or pilates, or cooling yourself off with a swim. You could also get out your home cricket nets for a fun game for a great excuse to invite your friends over for a sociable evening.


text:
This category contains special pages for which there is no visible menu item; it is attached to the Hidden menu.
For all blocks regarding events
– Marianne Orchard
There's a wok restaurant with an all-you-can-eat buffet in the next village. The kids love it. It has sushi alongside stuffed eggs alongside huzarensalade alongside loempia's alongside chicken nuggets along-side babi pangang alongside chips alongside soesjes alongside chocolate fondue alongside lychees along-side ice cream. It's a Chinese restaurant that caters to Dutch tastes. It's safe exotic without being too apart, as my mother-in-law would call it. So it's a Dutch version of Chinese, which means that, in contrast to British Chinese, babi pangang is a standard part of the buffet. The main thing is that there is no meat-and-two-veggery and not a boerenkool in sight.
I think this is a good light in which to see the version of English that crops up in the Netherlands often, which we native speakers like to laugh at, because it isn't the English we speak.
Exotic but not too apart
You know the kind of things; Albert Heijn's now discontinued Euro Shopper line is a classic at them – 'Puff pastry with meat filling' for saucijzenbroodjes, when sausage rolls would be what the British native speaker would say; 'Short cake biscuits' for spritsen, when the British native speaker would say Viennese
swirls (and the Austrian in turn would probably say something else); and 'rusks' for beschuit.
And there's the insistence, HEMA is a particular offender, in using the term 'Old Dutch' when it doesn't have any significance to native-English speakers because we don't know – and if we do know it's a sign we've been living here too long – that it's a literal translation of Oud Hollands and for a Dutch person invokes nostalgic images of when life was simpler, when we were less druk, when we wore newspaper trousers and clogs, and counted our blessings.
But these products aren't aimed at the native speaker of English, so it's none of our business whether it means anything to us because that's not the point. It's just like the Chinese restaurant isn't aimed at Chinese people. It's about giving a feeling of some-thing being exotic but exotic within reason, exotic that we can understand.
It's within these kaders that we should attempt to understand that old Dutch Eurovision entry, 'Birds' by Anouk. The whole time I was thinking 'huh, I don't get why she's singing about birds falling from the roofs', even if I disregarded the niggle that surely they can't fall down the rooftops but should be falling from the rooftops. They could fall down the roofs but not down the rooftops, but that wouldn't scan in the song. This was just a pernickety niggle compared with the bigger one of what's going on with all those birds? My husband solved that conundrum by saying in one GUEST BLOG
22 eSense 42 | 2016
those voices that imply that what he is saying is obvious, 'It's a saying: vogels vallen dood van het dak. You say it if it's really hot. Then it's so hot that the vogels vallen dood van het dak.'
However, now I've looked up the lyrics to the song it still doesn't make much sense to me. But maybe there are layers of meaning that a native speaker of Dutch would understand just as they would understand why you need stuffed eggs and huzarensalade with your sushi.
Ben ik al aan de bird is a Dutch bilingual pun. 'Bird' sounds like beurt meaning 'turn'. So Anouk is saying 'Is it my turn yet?'
Exploding rookworst
We had rookworst recently. It wasn't just any rookworst but an ambachtelijke rookworst. As an ambacht is a trade or craft, it would have been a pleasant surprise if Sir Ambachtelijke Rookworst of the Poiesz had been wearing a codpiece and carrying a gourd in true tradesperson fashion (my anachronistic view of medieval fashion).
But let's step away from talk of sausages and codpieces before it all degenerates into something unseemly and instead turn our attention to the word ambachtelijk. As I said already, ambachtelijk comes from ambacht, as in trade or craft. So it has the same meaning as the English artisan, as in an artisan bakery. However, it has reached a point in its evolution where if it is being used about rookworst sold in a supermarket we can be sure it doesn't hold much meaning but is being used instead to convey an emotion.
Ambachtelijk can therefore signify a product that has been handmade by a skilled tradesperson. But it can equally signify one that has been developed in an industrial process to resemble a product that has been made by hand by a skilled tradesperson. Or it can signify any old crap that the marketing department thinks will sell better with its addition.
Our rookworst ended up looking less than ambachtelijk after it exploded in the pan because I forgot to prick it. Which is symbolic of ambachtelijk because the word has become so full of meaning that it has exploded and now means nothing.
Words don't come easy
I thought this post could do with some musical accom-paniment from the 1980s. So here it is: Words by F.R. David. And, how apt, words not coming easily is the focus of this post. Because they don't. Come easily. Words, that is. In conversation anyway. Writing is a different kettle of fish because it gives me time to think and revise and think and revise and leave things to stew for a bit. With conversations, though, I find that if I'm speaking English, Dutch words (and the odd German word, but this is very rare) will jump in and try to clothe my thoughts – a bad metaphor perhaps because it makes it sound as if my thoughts are obscene when they're probably not obscene enough.
It's the same if I'm speaking Dutch, but this time it's the English (and occasional German) words that are doing the decent thing. What I've concluded is that when I speak Dutch I'm pretending to be someone who speaks only Dutch and when I'm speaking English I'm pretending to be someone who speaks only English when really I'm someone who speaks both languages and needs both of them to come out with anything coherent.
So if we look at the process, a thought forms and it wants to be clothed in some words. Like in that sinister Amazon warehouse in the UK where the workers are treated like robots, the order arrives and the message goes out to the workers that some words need to be picked.
The system and drones in my brain, however, are a lot crapper than the Amazon system. So the order comes in and the workers scurry off without bothering to check whether the product needs to be in Dutch, English or, on rare occasions, German. They scramble and come up with anything in either language that looks like it might just do, often producing a word that doesn't match the original request anyway. And this is OK if I'm talking to someone like me who functions in both languages, because I can then say things like, 'Yeah it was the avondvierdaagse last week and it wasn't too vreselijk and was actually quite gezellig but we've got a verjaardag this weekend and that's going to be really doomy and all oh lekker kopje koffie-ish…'
So we actually have our own language, which is neither Dutch nor English but both. And it's a lot easier to speak than Dutch or English separately. I don't know how polyglots do it.
All SIG related events are visible for members only.
SENSE organizes an annual programme of events. The professional development events call upon the knowledge and experience of the Society's seasoned members, including such luminaries in the field as Mike Hannay and Joy Burrough-Boenisch, as well as the expertise of guest speakers such as English lexicographer Susie Dent and Emma Wagner of the European Commission's Translation Service. Rounding off these events is a lighter programme of social functions for everyone in the Society's widely dispersed community. All events and functions are open to members on a first-come basis. Non-members may apply to attend to select events two weeks after registration has opened to members.
Thank you for your registration. The payment is succesful.
Return to the SENSE 25 Years Jubilee
This privacy policy is applicable to members, people who contact the Society and visitors to our website www.sense-online.nl.
The Data Controller for personal data processing by SENSE and on this website is:
SENSE the Society of English-language professionals in the Netherlands
Registered address:
De Geestlaan 10
2361 XX Warmond
You can contact our Data Protection Officer by e-mail at email@example.com.
Personal data that we process
We do not collect any personal data from people who visit our website. However, we collect some or all of the following personal data from visitors who contact us with a query or register to attend an event as a non-member:
From our members we collect the following information:
Why do we collect personal data?
We collect this information to provide our products and services to our members and visitors and to:
Storing your data
Your personal data is stored in the Netherlands and is not shared with any third party. We store your member data for the duration of your membership and for up to seven years after your membership ends. You can inspect or change your data at any time by logging into our website and going to your profile.
Visitors to the SENSE website can look in our member register to find a freelancer. They will only see your personal data if you have opted to participate in our freelance register. If you do not want to share your data with persons outside the Society, you can opt out of the freelance register by updating your profile.
Members can view your personal data in the membership directory.
We use cookies on our website to enhance your browsing experience, to assist with social media use and to help us improve the site. We use Google Analytics.
Social media buttons
Our website includes buttons that can be used to promote our website pages on Facebook, LinkedIn and Twitter. These buttons use snippets of code originating from those social networks which place cookies on your device. We do not have any influence on this process.
Viewing, changing or deleting your data
You have the right of access to your personal data and you can change or delete many of the details yourself in 'My Profile'. If you are no longer a member of the Society, you have the right to request we delete your data. Send an e-mail with your request to our Data Protection Officer.
Keeping your data safe and secure
SENSE endeavours to keep all personal data strictly confidential and will actively take measures to keep personal data secure from unauthorized access and theft. We aim to comply fully with the General Data Protection Regulation (GDPR).
If you have a complaint about our use of personal data, please let us know by contacting our Data Protection Officer at email@example.com.
Under the GDPR you have certain rights concerning your personal data. You may exercise these rights by writing to our Data Protection Officer at email@example.com.
If you do not agree with our use of your personal data and would like to lodge a complaint or would like the Dutch Data Protection Authority (Autoriteit Persoonsgegevens) to mediate with us, you can contact them via their website: www.autoriteitpersoonsgegevens.nl
This privacy statement was last updated in May 2018 and it will be reviewed and updated when necessary.
You could go back to our home page. Or perhaps try our search function.
If you are a member of SENSE, you could go to the forum.
If you clicked on a broken link, please let us know: email@example.com.


text:
Release Notes ZynBox v. 18.104.22.168
Application: ZynBox for Outlook
Release Version: 18.104.22.168
Release Date: 11-21-2018
Environment: Production
The production release is available from app.zyn, but you can install direct from the link below. https://zynbit.box.com/s/a4holbco8si6j4t8p2yqs8im4q0j615n
- Currencies are formatted with two decimal places in Compact and Search layouts.
- Consider all closed status values (rather than just the default) for Case, Opportunity, Task and lead objects.
New Settings Available to change ZynBit experience
- Hide the plus icon (add object) for any object list that appears under an expanded Contact. Example – prevent user from adding a Case to a Contact.
- Hide any object list that appears under an expanded Contact. Example – user wants to override Salesforce settings for related lists viewable on contact.
- Configurable Add Product URL.
- Specify an alternate object and record type for Log Call.
- Only consider Shared Calendars (ignore My Calendars and Other Calendars) when setting up delegated calendar monitoring.
- Disable Email Filing setting will hide Quick File box when the user creates an object. (This box is typically used to file the email at the same time that the new record is created.)
- Hide the Arrow that is usually prepended to the subject of a filed email. (Two arrows currently appear – one facing right for reply and facing left for send)
Bugs Fixed
- Creating an object while not viewing an Outlook item shows an error popup about attachments. Example – select a date header in your Inbox and use the Create New tab.
- Unable to click File Email after composing an inline reply and then clicking Pop Out.
- Searches requested in batches of 150 object types rather than 200.
- Office Add-Ins are disabled when composing a new email.
- Task Type field is not sets for some users when filing an email.


text:
Price For Life Internet withUnlimited Nationwide Calling up to 100 Mbps
Speed may not be available in your area.
Additional taxes,fees, and surcharges apply. Rate excludes CTL Fees not to exceed $3.00/mo/line.* Offer Details
- Get the fastest Internet speed available at your home.
- Stay connected with Home Phone Unlimited Nationwide Calling.
Special Mover's Deals
MOVING? Get special mover's deals on Internet & Phone!
Speed may not be available in your area.
Additional taxes,fees, and surcharges apply. Rate excludes CTL Fees not to exceed $3.00/mo/line.* Offer Details
- Get the fastest Internet speed available at your home.
- Stay connected with Home Phone Unlimited Nationwide Calling.
CenturyLink Deals
Get High-Speed Internet and Home Phone and enjoy a complete home experience at a great price.
Price For Life High-Speed Internet
$49. /mo
for all speeds up to 100 Mbps. Rate requires paperless billing. Additional taxes, fees, and surcharges apply. Get the fastest internet speed available at your home (max speed is up to 100 Mbps).* Offer Details
- Speeds up to 100 Mbps (Speed may not be available in your area)
- No bundling needed!
- No Rate Hikes. No Contract!
- Keep your rate as long as you keep your Price For Life plan.
Fiber Gigabit Bundle
Speed may not be available in your area. Maximum download/upload speed of up to 940 Mbps via a wired connection.
$85. /mo
Additional taxes, fees, and surcharges apply. Rate excludes CTL Fees not to exceed $3.00/mo/line.* Offer Details
- Fiber Gigabit (Speed may not be available in your area. Maximum download/upload speed of up to 940 Mbps via a wired connection.)
- Home Phone Unlimited Nationwide Calling
- No Rate Hikes. No Contract!
- Keep your rate as long as you keep your plan
CenturyLink High-Speed Internet delivers the speed you need, so you can connect the way you want to. You'll also enjoy the ultimate in In-Home WiFi experience that delivers a fast Internet connection throughout your home, and allows you to connect multiple devices to your home Internet network wirelessly, giving you the freedom to stream and game with ease. Best of all, several CenturyLink Internet plans come with Price For Life, so your Internet rate won't change as long as you keep your CenturyLink Price For Life Internet plan—that means fast speeds with no contract, and no rate hikes.
CenturyLink Phone offers a Home Phone service solution at a low price.
You'll enjoy a clear connection to call your friends and family across the nation, with no delays, and no lag time. Unlike mobile phones, CenturyLink Phone doesn't rely on cell towers, and therefore offers a reliable, stable connection, plus having a Home Phone is an important safety feature for your family—many home security systems require a landline for security monitoring and, in an emergency, your landline phone number helps first responders identify your location. CenturyLink Phone also offers International Calling Plans, and includes popular calling features.
Get the CenturyLink Internet and Home Phone Bundle that's perfect for you, and start saving today.
Moving to medford, oregon?
Here are some helpful links to get you started.
About: medford, oregon
Named after Medford Massachusetts the city began with a group of railroad surveyors in 1883. The city's economy was originally focused on agriculture though today it deals primarily with the health care industry. Alba Park, the oldest city park, hosts the Pear Blossom Run every year along with an all day fair. The city's Bear Park is also very popular due to its ground containing not only tennis courts but a skate park and a BMX track but a dog park and amphitheater as well.
Population: 76850
Local Newspapers: The Mail Tribune
Local Sports Teams: Sothern Oregon Spartans
Local Colleges: Southern Oregon University
Quick Links and info for Medford, Oregon
City Info
Local: (541) 774-2250
Emergency: 911
Local: (541) 774-2300
Emergency: 911
Medford City Hall
411 West 8th St., Medford, Oregon 97501.
Phone: (541) 774-2000
Natural Gas Hook-ups and Disconnects
Avista Utilities
1301 Center Drive, Medford, Oregon 97501.
Phone: (800) 227-9187
Water Hook-ups and Disconnects
Medford Water Commission
200 South Ivy St. - Room 177, Medford, Oregon 97501.
Phone: (541) 774-2430
Trash Removal Schedule:
Pick up and Recycling schedule
Pacific Power
2080 W. Main St, Medford, Oregon 97501.
Phone: (888) 221-7070
Ramada Medford and Convention Center
2250 Biddle Road, Medford, Oregon 97504.
Phone: (541) 779-3141
The Chamber of Medford/Jackson County
101 East 8th Street, Medford Oregon 97501.
Phone: (541) 779-4847
Local Airport
Rogue Valley International-Medford Airport
1000 Terminal Loop Parkway, Medford, Oregon 97504.
Phone: (541) 772-8068
Area Hospital:
Providence Medford Medical Center
1111 Crater Lake Ave., Medford, Oregon 97504.
Phone: (541) 732-5000
Local Organizations:
522 West Sixth Street, Medford, Oregon 97501.
Phone: (541) 772-6295
Area Colleges
Rogue Community College
117 S. Central Avenue, Medford, Oregon 97501.
Phone: (541) 245-7500
The RRRink
1349 Center Drive, Medford, Oregon 97501.
Phone: (541) 770-1177
Points of Interest
History & Discovery Center - Downtown Medford
106 N. Central Avenue, Medford, Oregon 97501.
Phone: (541) 773-6536
Have a local business that you would like us to link to? Visit our Facebook Page and send us a message!
Zip Codes associated with medford, oregon:
97501 97503 97504
Towns/Cities near medford, oregon : Ashland Talent Phoenix Jacksonville Central Point White City Eagle Point Rogue River Grant Pass
CenturyLink Internet and phone bundles are available in Medford, Oregon. When you bundle high speed Internet and Home Phone services into one package, you will enjoy great discounts and the convenience of just one monthly bill. CenturyLink offers residents of Medford, Oregon the chance to save big while enjoying the latest digital home service technology. If you're in the market for Internet and Phone and live in Medford, you can take advantage of great CenturyLink deals. Search and compare CenturyLink offers in Medford easily and start saving on digital home services today. CenturyLink Internet lets you surf and stream the way you want to. Residents of Medford can enjoy buffer-free Internet access and select from a number of high speed internet packages. Plus, you will get a reliable and secure connection. CenturyLink Home Phone is a great addition to Internet service, and let you talk more for less. What's more, it comes with unlimited nationwide text, a feature that saves you money on cell phone messaging. When you bundle digital home services, you enjoy bigger savings and all the benefits of CenturyLink Internet and Phone.
CenturyLink High Speed Internet service is available in Medford, Oregon. Residents of Medford, Oregon can sign up for great deals on CenturyLink Internet and enjoy faster download speeds, big savings and the comfort of Internet Security software. What's more, CenturyLink High Speed Internet service enables Medford residents to select the Internet Package that best suits their needs. Whether you and your family simply require a basic connection to surf the web and check email, or you like to stream, game and watch TV online, CenturyLink has an Internet package for you. Plus, With the freedom to choose a great Internet plan and the peace of mind of knowing that your connection is secure, CenturyLink Internet offers Medford a great Internet product, for less.
CenturyLink Home Phone packages let Medford residents to connect for less. Phone packages from CenturyLink bring unlimited nationwide calling, for less. Gone are the days when you hear phone crackling and guess what the person you're talking to is saying - CenturyLink Phone service offers crystal clear calling, and a multitude of popular phone features for an affordable monthly price.


text:
Binghamton, NY, (WiredPRNews.com) Upstate New York based electronic cigarette company "Amerismoke" is one of the leading premium electronic cigarette brands on the market. Their new two-piece design is catching eyes everywhere, simplifying the process while still maintaining the satisfying experience you've come to expect from Amerismoke.
Specifically, Amerismoke now offers electronic cigarettes that combine the nicotine cartridge and the atomizer into one disposable unit. Every disposable cartridge contains its own atomizer, which means that you will get peak vapor performance with every cartridge. Not only that, but there is no confusion about how to assemble the two piece e cigarette, simply screw the atomized cartridge onto the charged battery and everything is ready to go.
For those new to the electronic cigarette, it is a small battery powered device that looks almost identical to a real cigarette. By utilizing advanced vaporization technology, the e cig substitutes smoke with an odorless vapor as a means of nicotine delivery. The electronic cigarette was designed to simulate every aspect of traditional tobacco cigarettes, while leaving out everything undesirable associated with tobacco. For instance, the e cigarette does not contain any of the tar, carbon monoxide or toxins that tobacco does. The electronic cigarette's vapor is completely odorless and is not considered secondhand smoke, which means that the e cig can be used anywhere in public, even where smoking has been banned.
Aside from those advantages, Amerismoke is also a pioneer in setting the highest production and manufacturing standards in the industry. All of Amerismoke's e-liquid is lab tested right here in the United States to ensure that the ingredients are precisely what they should be and that there are zero impurities. They have gone above and beyond to provide only the highest quality e cigarettes for their customers and that is the basis for their exceptional reputation.
If you would like more information on Amerismoke Electronic Cigarettes, or to try one for yourself visit their website at http://www.Amerismoke.com or call 1-877-324-4462 to speak with a representative.


text:
April is National Car Care Month and Jake Sweeney FIAT is the FIAT dealership you can turn to for all your car care needs! A few easy and simple checks, fill-ups and inspections can make a huge difference when it comes to keeping your car comfortable on the road and keeping you and your passengers safe.
And that's not all! Good car care is incredibly beneficial for several reasons. In addition to making the vehicle safer, it also helps to prevent costly repairs and replacements, improves fuel efficiency so you save at the pump, and helps you to maintain vehicle value so you can get the price you deserve if you decide to sell later down the line.
So what's the best way for you to celebrate this National Car Care Month? By visiting the service center here at Jake Sweeney FIAT to get your FIAT 500L or FIAT 124 Spider the maintenance it deserves. Our team will begin by checking your tire tread and alignment, to make sure your tires are wearing down at the same rate. They'll perform an oil check or change if necessary, as well as checking the brake fluid, transmission fluid, and power steering fluid.
After that, your technicians may check your battery's power, as colder temperatures can result in lower battery efficiency. They'll also test out your brakes and emergency brake to make sure that your vehicle's capable of stopping quickly and efficiently. Hoses and belts should be checked often for signs for damage or wear and replaced if necessary.
When it comes to expert, reliable car care, parts, and information, Jake Sweeney FIAT is the place you can rely on. Visit us at 5969 Centennial Circle in Florence today to keep your car safe and secure for many more tomorrows to come.


text:
The National Association of Graduate Teachers (NAGRAT) has served a very strong warning that members found working in the course of the ongoing strike action declared Thursday would be chased out of the classrooms.
NAGRAT executives in the Upper East region issued the warning Friday at a news conference convened in the regional capital, Bolgatanga, to announce members of the association in the region had joined the boycott action declared by the national executives in the national capital, Accra, on Thursday.
"Yesterday, our national [executives] declared the strike and we are having ours today. We are going to give support, we are going to reinforce [and] we are going to intensify the strike action in this part of the country.
"After this declaration in support of the national, we the regional officers and the zonal officers will do monitoring and any teacher that is found in the classroom, we'll chase that teacher out. If we all have said that we are declaring a strike, they must go with it. What is due us, we have to fight for it!" affirmed the NAGRAT's Upper East Regional Chairman, Samuel Atompoya, at the conference.
The aggrieved teachers' fraternity took industrial action over what they describe as protracted delay in giving out promotion letters to some teachers who were promoted some years ago. Mr. Atompoya echoed on Friday the reasons articulated on Thursday by the NAGRAT's President, Eric Angel Carbonu, as to what led to the job action.
"Today, teachers who have been promoted as far back as three years ago have not been given their promotion letters indicating the effective date of their promotions. This will affect their chance of progressing in the GES.
"Teachers who have additional responsibilities besides their teaching duties have not had their responsibility allowances restored after they were promoted. Some teachers have suffered this problem for the past three years. Some teachers who have completed their study leave with or without pay have had difficulty being reinstated into the Ghana Education Service (GES) and even when they are reinstated, they are still being paid their pre-study leave salaries," Mr. Atompoya reiterated.
We downed our tools as Government wasn't ready to meet us— NAGRAT
Among the concerns that prompted the ongoing strike action also is alleged delay in inviting teachers, who are due for upgrade after serving on their current ranks for a required four-year period, for promotion interview.
The number of affected teachers who have been glued to the same ranks for more than half a decade is so colossal NAGRAT says there is "a huge backlog" of teachers waiting for just a call for the promotion due them.
"It is a nightmare to be transferred or to seek transfer in the GES these days. The GES is unable to effect genuine transfer requests due to impediments placed in its path by the HRMIS (Human Resource Management Information System) of the Public Service Commission. This has brought untold pressure and hardship on teachers in the service. We call on the Public Service Commission to remove the obstacles it has placed on the path of the Ghana Education Service. It looks like the government has suspended its obligation of paying salary arrears. Yet, day in day out, new arrears are being created.
"The promotion issues and salary upgrade problems mentioned earlier have created another batch of salary arrears. Teachers who, for one reason or the other, have had their salaries stopped will have to live without being paid for months despite the fact that they report regularly to duty. It is clear that the HRMIS being operated by the Public Service Commission is incompatible with the scheme of service of the GES. The teacher unions, since the inception of the HRMIS, have complained about the system to no avail," Mr. Atompoya added.
Fielding questions from the media, the NAGRAT members in the region said they resorted to downing their tools because government chose to ignore them when their concerns were raised in July, this year, at a news conference.
"The Ghanaian public knows that these are issues that are facing us. But government is not ready to meet us. We have to lay down our tools for them to know that we are serious," the association's Regional Chairman stated.
By Edward Adeti


text: Politics Political news Monroe County Leaders Bring Lionfish, Rum Punch And Affordable Housing Concerns To State Legislature By Jessica Bakeman • Mar 20, 2019 Jessica Bakeman / WLRN News Hundreds of people enjoyed food, drinks and music from the Florida Keys at the state Capitol on Tuesday evening, the culmination of an annual lobbying trip that Monroe County elected officials and business owners use to share local priorities with state lawmakers. City mayors, school board members, community college administrators and restauranteurs told legislators throughout the day about their biggest concern: affordable housing. Close Trump Ally Joe Gruters Now Leads the Florida Republican Party By Chris Remington • Jan 17, 2019 Miami Herald Republican State Senator Joe Gruters, representing Florida's 23rd District, is the new President of the Florida Republican Party. Gruters won handidly during the annual GOP conference in Orlando last Saturday, defeating Charlotte County Republican Bob Starr. South Florida Legislators Take On New Leadership Positions In House Democratic Caucus By Jessica Bakeman • Dec 27, 2018 Robert Hunter / Courtesy of the Florida House of Representatives A few South Florida Democrats will have new leadership positions in the state Legislature when session starts in March. Rep. Kionne McGhee, a Democrat from south Miami-Dade County and the incoming minority leader in the Florida House, created four new deputy positions. Three of them represent South Florida. Each new deputy minority leader will oversee a group of House members; the Democratic caucus of 47 members is divided roughly along geographic lines. There are a total of 120 members in the House. As Election-Recount Acrimony Ends, New Two-Year Cycle In Florida Politics Begins By News Service of Florida • Nov 23, 2018 Florida Senate via Twitter Florida's political world is built around two-year cycles. And the latest acrimonious, head-scratching cycle finally ended Tuesday when the state Elections Canvassing Commission certified the results of the Nov. 6 elections. The acrimony, of course, did not end with the usual campaign attack ads and nastiness. It lasted nearly two additional weeks as ballots continued to be counted and recounted and legal fights raged. Florida Democrats Eye Climate Change, Gun Legislation With New House Majority By Steve Contorno • Nov 23, 2018 Wilfredo Lee / Associated Press via The Miami Herald Florida Democrats in Washington could only watch when the Republican Congress decided against new gun legislation after a shooter killed 17 students and staff at a Parkland high school last year. Nor could they force action to combat rising sea levels off Florida's coastlines. That will change come January. After gaining 38 seats in the U.S. House of Representatives, including two in Florida, Democrats will control the lower chamber for the first time since 2011. Election Changes Eyed As 2018 Results Finalized By News Service Of Florida • Nov 20, 2018 Christian Colón / Miami Herald After months of mudslinging, weeks of court wrangling and days of ballot counting that again landed Florida in an unwelcome national spotlight, a state panel matter-of-factly finalized the 2018 election results in a five-minute meeting Tuesday. The certification came on time, but problems with other election-related deadlines in two large, heavily Democratic counties --- Palm Beach and Broward --- are prompting county supervisors and legislative leaders to ponder possible solutions to the state's ballot-box woes. New State House Speaker From Miami Pledges Civility, Takes Aim At Regulations By News Service of Florida • Nov 20, 2018 Elizabeth Koh / Miami Herald Miami Lakes Republican Jose Oliva called for civility among lawmakers as he vowed to continue his party's push for limited government after being formally elected Tuesday to serve as House speaker for the next two years. Ron DeSantis Hire A Democrat? After Election, A Shift In Tone By Emily Mahoney • Nov 20, 2018 Mark Foley / Florida House of Representatives Upon winning the Florida governor's race, Ron DeSantis had firmly established himself as an unyielding partisan who's an ardent defender of President Donald Trump and a relentless prosecutor of his Democratic opponent, Andrew Gillum. But in the two weeks since Election Day, the 40-year-old former congressman has been tinkering with his brand. Caldwell Concedes Cabinet Race To Fried By News Service Of Florida • Nov 19, 2018 Meredith Gettings / Florida House of Representatives Republican Matt Caldwell pointed to a need for political "sanity" and a "peaceful transfer of power" Monday as he conceded the race for Florida agriculture commissioner to Fort Lauderdale lawyer Nikki Fried, the only Democrat to win statewide this year. Caldwell, a real-estate appraiser from North Fort Myers who has served the past eight years in the Florida House, said in a statement that he still had questions about how ballots were handled in Palm Beach and Broward counties, where he had filed a lawsuit. But he didn't want to use "legal loopholes to win an election." ACLU Florida Chief Leaves On The 'Highest Note' — Restoring Felons' Voting Rights By Sanya Mansoor • Nov 19, 2018 Courtesy of ACLU Florida After 40 years of leading American Civil Liberties Union chapters in Michigan and Florida, Howard Simon is calling it quits. He'll remain in Florida, and plans to stay active, but will relocate from Miami to the Gulf Coast. The end of a long career with such a venerable organization might normally elicit polite plaudits from across the political spectrum. But that's not necessarily true when it's the ACLU. New Forms Used To Cure Votes Cause Last-Minute Confusion At Election Offices By Danielle Prieur • Nov 19, 2018 Christian Colón / Miami Herald Florida voters whose mail-in ballots were rejected because of signature problems had the chance to fix those ballots over the weekend. Secretary of State Ken Detzner released a new affidavit form to cure vote-by-mail ballots with mismatched signatures. The canvassing boards decided whether to accept both versions of the form only hours before the deadline. Judge Orders Brenda Snipes To Grant 'Immediate Access' To Records After Gov. Scott Sues By Jessica Bakeman, Caitie Switalski & Nadege Green • Nov 8, 2018 Caitie Switalski / WLRN NEWS In Election Day Tradition, Gubernatorial Candidates Cast Votes For Themselves By Jessica Bakeman • Nov 6, 2018 Left: WLRN, Right: Ron DeSantis' Twitter The men who are vying to be Florida's next governor cast votes for themselves Tuesday morning, with Republican Ron DeSantis near Jacksonville and Democrat Andrew Gillum in Tallahassee. DeSantis, who represented a northwest Florida district in Congress before resigning his seat to focus on his gubernatorial campaign, went to the polls in Ponte Vedra Beach with his wife Casey early Tuesday morning. Emma González Urges Young People To 'Vote In Every Election Like It's Your Last' By Jessica Bakeman • Nov 5, 2018 Jessica Bakeman / WLRN Activist Emma González, who became famous after giving an impassioned speech in Fort Lauderdale days after the shooting at her Parkland high school in February, stood on the steps of Florida's old state Capitol building on Monday and urged people to vote. "Gun violence is on the ballot," González said. "Our lives are in the hands of the people that we elect. Vote in every election like it's your last, because it very well could be." DeSantis Campaigns At Miami Jewish Deli, Accuses Gillum Of 'Warmed-Over Anti-Semitism' By Jessica Bakeman • Oct 23, 2018 Jessica Bakeman / WLRN Republican gubernatorial candidate Ron DeSantis told voters at a Jewish deli in Aventura on Tuesday he'd be a champion for Israel if elected governor. Pages« first ‹ previous 1 2 3 4 5 6 7 8 9 … next › last »


text:
Mazzer Robur Electronic Conical Burr Espresso Coffee Grinder
The Mazzer Robur Electronic grinder is the pinnacle of high quality and high volume espresso grinding. Its 71 mm conical steel burrs and 900 Watt motor combine to provide 500 Rotations Per Minute. This means that the Robur can grind for a double basket in around 3.5 seconds. The best espresso grinder on the planet just got even better– the Mazzer Robur Electronic version now comes standard with "pre-seasoned" burrs! If you are looking for an manual dosing model, see our Mazzer Robur Espresso Conical Burr Grinder and Doser listing.
- Electronic Dosing reduces coffee waste and provides perfect doses.
- Stepless grind settings
- Dosage can be set in increments of 5 hundredths of a second (.05) via the controls on top of the dosing chamber.
- Conical Steel Grinding Burrs: 71 mm
- Burrs are pre-seasoned via a two stage sandblasting process.
- Coffee-bean hopper capacity: 4 lbs
- Heavy duty die-cast aluminum body.
- Color: Silver or Black (Choose after selecting "Add to Cart")
- Dimensions: 9.5" W x 12.25" D x 28.25" H
- Weight: 62 lbs
- Electrical: 110 Volt 900 Watt
- 1 year parts and labor
Rch Verified Buyer
5 / 5 stars
The other review of the Robur is completely wrong.
Was this review helpful? 1 1
Marius V. Verified Buyer
1 / 5 stars
Coffee grinder
Was this review helpful? 1 11


text:
When my wife and I bought our first home, we quickly realized that the central air had no ability to get cool air upstairs. After a handful of failed ideas, my wife got our top floor an air conditioning unit. Thankfully, this was different than the window mounted options seen in many older buildings, including our old apartments. This was one that had a hose mounted to a partially opened window but otherwise remained inside. In addition to the cooling breeze, it filtered air and provided a nice white noise at night.
At least, the sound of the air conditioner was usually soothing.
Not long after installation, I began to notice strange sounds at night. At first, it just sounded like the usual purr of the house appliances and the air unit. As time went on however, I started to hear music or voices being blown into the room with the filtered, chilled air. On more than one occasion I have turned off the air and listened, hoping to hear what the source was of the voices in an otherwise empty home. Everytime I would do this, a forced silence enveloped the room. The voice or melody died with the air blowing into the room.
This test has happened enough that I have stopped turning off the unit and now attempt to separate the voices from the artificial wind as I fall to sleep at night. Never at a clearly audible volume, it always happens at a level where the voices in my head could be either whispered or fabricated. This should terrify me more than it does, especially because my wife never hears it. Perhaps my side of the bed being closer to the device has made me the unwilling recipient of such communications.
We have a running joke that our house is haunted. From the ominous though tidy crawlspace to the baby monitor detecting sound and movement when no one is home, it has been chalked up to creepy coincidence and nothing more. If adding this air unit spewing near subliminal messages doesn't feel like a macabre environment, I'm curious what would.
It happened again last night. As I drifted off after some late-night reading, I felt I could once again hear voices. Barely noticeable, I imagined the electrics were pulling messages from another place. Possibly another dimension, world, or location on this plane of existence. Content to leave it at that, I take comfort in the words of the unseen as I rest my eyes at night.


text:
You can stay up to date with whats going on at the school by following us on Facebook, Instagram or Twitter. We love to share photos of the latest creations so you might see your work on there!
You can also join our mailing list to receive our newsletter, class dates etc straight to your inbox. Please sign up by filling out the attached form here
If you have any questions or would like to find out more about any of our courses please contact us using the form below.
We aim to respond within 24 hours; if you haven't heard from us please check your junk/spam folders.


text:
Have you met up with Sections from other Groups this year? Or taken part in District & County events?
Then enter yourself into the District Friendship Challenge!
There is a prize for the Scout Group who has taken part in the most activities between April 2017 and April 2018. The winner will be announced at our St George's Activity Day.
Email details of your joint activities to Linda DC to enter.


text:
Oct – Dec 2019 Saturday mornings.
REGISTER YOUR INTEREST HERE: (your contact details)
Learn to Sail – Juniors
Oct – Dec 2019 Saturday mornings.
REGISTER YOUR INTEREST HERE: (your contact details)
Tackers – Course Fee $215
A fun introductory, games-based sailing program designed for kids aged 7-12 yrs, delivered at recognised Tackers Centres, predominantly who are affiliated sailing clubs. Kids don't need to have any sailing experience or club membership to participate. All the equipment, including the boats, is provided.
Juniors – Course Fee $229
A club-based junior sailing activity for kids who have completed Tackers 2 or another junior program. It will include elements of the Tackers 3 curriculum. They will be sailing a tackers Opti, Pacer or other dinghy.
Important to the overall Junior program is the opportunity for children and their families to continue to participate in the social activities at the club and develop a sense of belonging within the club. Being part of Juniors should be as much about loving being at the club as it is about enjoying the sport.


text:
Salt Intake: Not too much, neither too less.
A new study in the American Journal of Hypertension which reviewed 23 studies covering over 360,000 patients found that it is bad to eat too much salt and it is bad to eat too little and that the right amount is between 1 and 2.5 teaspoons. They concluded "This robust body of evidence does not support universal reduction of sodium intake."


text:
Placing Your Order
Claim Your 20% OFF credit by signing up today! (limited time offer)
Already a client? login
Drop off your clothes in any available locker at your condo, with your front desk, one of our 24/7 public Laundry Concierge kiosks, or any of our other many locations. We have great "how-to-use" instructions at all our locations, so not to worry.
Enter any four-digit code and turn the handle to lock your locker.
Now text us at 647-361-6361 stating the service type you require and the locker number you claimed, such as dry clean 323 or login to your online account and get it done there.
Have special instructions? After we confirm your text order, text back "NOTES" along with your request. There's also a notes box when online ordering. This Concierge has you covered
You pick up your order at your convenience, anytime, day or night, and even forward the alert to a friend and they can pick it up for you.
Think Clean!


text:
We see them every day – someone drifting across lanes, running through stop signs or driving well below the speed limit while talking, texting or checking something on their smartphone. [Read the full story.]
Share this post
I've been working in claims for a long time. I've listened to countless pitches about automating the claims process. [Read…
March 16, 2020
Read more
The time between Memorial Day and Labor Day are usually marked by summer vacations, but they're also considered by some…
May 30, 2019
Read more
Insurtech is still a relatively young sector defining assumptions. [Read the full story.]
May 30, 2019
Read more


text:
The McDaniel Family
Gordon Family YMCA
We are the McDaniel family: Matt, Marjorie, Jojo, and John. My husband, Matt, started judo when he was eight-years-old at Highline YMCA. Originally, his parents put his older brother in the class to learn to protect himself from bullies. They noticed that judo taught their kids more than just self-defense; it also taught them discipline, character building, recreation, and exercise. Soon all the siblings were involved in judo.
Matt competed both nationally and internationally. He has been sharing his passion for judo with his fellow judoka by volunteering for many years in different clubs. Now he's back to where he started the YMCA with his younger brother Tim sharing his passion for judo with the Sumner community.
My kids and I soon joined in, too, and judo became a family affair. Our daughter, Jojo, has grown so much through judo. She, who was once too scared to get on the mat at first, is now competing fearlessly at the national level. The sport helped her gain confidence, physical, and mental strength, while having fun. John (16 months old) is starting to get comfortable on the mat and is enjoying the presence of other kids and family.
It's a good way to spend time together, learn skills, get some exercise, and make friends.


text:
In a Nutshell
This quilt and pillow sham set is designed to beautify the bedroom and make it more cozy
Product Details
Set Includes :
- 1 quilt
- 2 pillow shams (one pillow sham for the twin-sized set)
Quilt and Pillow Sham Set :
- 100% polyester
- Available in several models to give an elegant touch to the bedroom
- Machine washable
- Designs: elephant, Clarissa, joliette, Anton
- Colors: red, blue, light blue, gray
Dimensions :
|Full / Queen
|86" x 68"
|86" x 86"
|90" x 120"
|Pillow sham
|26" x 20"
|26" x 20"
|36" x 20"
The Fine Print
Purchase: Limit of 4 coupons per person.
Shipping: Within 10 business days of purchase, in Quebec only.
Taxes and shipping included.
Order: At checkout you will be prompted to enter a shipping address. This cannot be modified once the order has been placed.
Offer available while quantities last.
For more information, see the rules that apply to all deals.


text:
Sorry! But you must be logged in to send a query or ask a question on SA9011 - One Million Years B.C. - Tumak (15 cm Soft Vinyl Statue) (Deposit Required).
Click here to login, if you are already registered or register here, if you do not have an account with us.


text:
About us
Our business philosophy
Our mission and keys to success
Our team
Our Brands
fantasy FLIRT Cosmetics
fantasy FLIRT Hygiene
NORMAL clinic
NORMAL clinic BABY
men Agressia
News & Events
Contact us
Hide menu
Try new product- Baby wet wipes NORMAL clinic BABY.
Credo-Capital Production LLC has developed a new line of hygienic products NORMAL clinic
Beautyworld Middle East, 2016
Wet wipes NORMAL clinic BABY are specially developed ...
New multi-function hygienic underpads NORMAL clinic contain a superabsorbent, which transforms the liquid into the gel and keeps a specific odour inside...
Credo Capital Production will take part at Beautyworld Middle East, 2016
Read more...
Read more...
Read more...
For HER
Hair Color Cream fantasy FLIRT
Personal care – fantasy FLIRT
Personal care – NORMAL clinic
For HIM
Shaving & After shaving
Hair care
Shower & Body
Gift sets
For your baby
Baby wet wipes
© 2011 Credo Capital. All Rights Reserved.
Vision of


text:
Известия Саратовского университета. Серия История, Международные отношения. 2014. №3
File downloaded: 425 times.
The number of page views: 244
Russian History
«The war on literature barricades»: legal cases against press in russia during the years of the first russian Revolution [PDF] 9
The preservation and researching of the monuments of everyday culture: the Yury Ozarowsky collection [PDF] 15
World History
International Relations
Russian-American Relations in the Caspian Region during a New Stage of the Neoconservative Strategy (2003) 76
Turkish Foreign Policy in the First Decade of XXI Century: the Experience of Mediation Efforts [PDF] 81
Regional History and Local Studies
Economic development of german Сolonies on the Volga river in the second half of the 18th – the first half of the 19th centuries [PDF] 98
The Manuscript Heritage by the Bishop of Saratov and Tsaritsin Eparchy Jacob (Vecherkov) in the Russian Archives [PDF] 109
Changes in the structure of the population of the Saratov Volga region during the Great World War (1941–1945) [PDF] 114
Critics and Bibliography
Book Review: N. S. Krelenko. «The image of the English revolution in the public of XVII–XX century» [PDF] 121


text:
19. December 2018 - News and Notifications
Landsbankinn has decided to endorse UNEP FI's new Principles for Responsible Banking, intended to align the banking industry with the Sustainable Development Goals (SDGs) and the Paris Climate Agreement.
The Principles were introduced at a meeting of UNEP FI in Paris in November 2018 and Landsbankinn is the first Icelandic bank and among the first endorsers of the undertaking. The Principles for Responsible Banking have been sent for global public consultation and will first be available for signature in September 2019.
The Principles were developed by 28 international banks under the auspices of the United Nations Environment Programme Finance Initiative (UNEP FI) of which Landsbankinn is already a member. UNEP FI is a global partnership between UNEP and 200 financial undertakings, focused on examining the impact of the financial system on the environment and society.
The Principles set the global standard for what it means to be a responsible bank and will ensure that banks create value for both their shareholders and society. They provide the first global framework that guides the integration of sustainability across all business areas of a bank, from strategic to portfolio to transaction level. The transparency and accountability mechanisms in the Principles require signatories to manage what matters most, set public targets and report back on progress.
Eric Usher, head of UNEP FI: "Today we welcome the first official endorsers of the Principles for Responsible Banking. I am delighted that the Principles are already gathering support as we know that we need to see urgent action to address climate change and the other social and environmental challenges the world faces."
Lilja Björk Einarsdóttir, CEO of Landsbankinn: "Landsbankinn aims to be a leader in corporate social responsibility in Iceland and we are very pleased to be amongst the first to endorse the Principles for Responsible Banking. We have worked with local organisations on social responsibility, we are a founding member of an investment forum for responsible investments and our own policy on social responsibility is shaped with comprehensive input from business segments to ensure that it is integrated with core banking operations. We find that the Principles for Responsible Banking provide us with a common language on how a bank can communicate these efforts and differentiate itself, which is important for our customers, employees and shareholders."
The six Principles for Responsible Banking:
Announcement from UNEP FI
News and Notifications - 07 May 2020 04:55 PM
In the first three months of 2020, Landsbankinn's after-tax results were negative by ISK 3.6 billion, compared with an ISK 6.8 billion profit for the same period in 2019. Loan impairment amounted to ISK 5.2 billion in the period, compared with impairment of ISK 1 billion in the same period last year.
News and Notifications - 24 April 2020 06:00 PM
S&P Global Ratings has today announced a lowering of Landsbankinn's long-term credit rating. The credit rating is lowered by one notch, to BBB/A-2 with stable outlook.
News and Notifications - 22 April 2020 03:03 PM
The Annual General Meeting of Landsbankinn was held on 22 April 2020 at the Bank's headquarters in Austurstræti 11, Reykjavík. At the AGM, Helga Björk Eiríksdóttir, Chairman of the Board of Directors, presented the report of the Board of Directors, and Lilja Björk Einarsdóttir, CEO, spoke of the Bank's solid standing and good performance in 2019.
Landsbankinn hf. Austurstræti 11, 155 Reykjavík, Reg. No. 471008-0280
Swift/BIC: NBIIISRE Rules Legal notice Personal Data Protection Policy
Contact us
Please use this form to send us queries, comments, compliments or complaints.
Please complete the form to make an appointment.
Use this form to request an interview with our advisors.
Appointments can be made for both branch visits and phone consultations.


text:
Change - Text Block 2
Decision making
On your toes
Effective change is about agile decision making. That only comes through being connected to the here and now while reading the signs of the changes ahead – a difficult balancing act. Ask any high performance athlete or leader and they will tell you that split second decision making is honed through practice. Experts know that large change is influenced by making many small ones effectively, which is why leaders need to be able to think on their feet and act with clarity. We refine the instincts and responses of our client's leaders.