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June 10, 2013
Media Advisory--Legislators to Visit Autism Center
Two Southern Illinois legislators will visit Southern Illinois University Carbondale's Center for Autism Spectrum Disorders at 10 a.m. Tuesday, June 11. State Sen. Dave Luechtefeld, R-Okawville, and Rep. Mike Bost, R-Murphysboro, are making the visit to draw attention to the prevalence of autism and ongoing efforts at the University to make a difference for those with autism, as well as their families and caregivers.
Reporters, photographers and news crews are welcome to cover the legislators' visit to the center, located at the Rehabilitation Institute in the Wham Building, Room 124. The legislators will tour the center and discuss autism and what the center does to assist children with autism, their families and professionals in the autism field.
The center, providing valuable services since 2003, assists children from ages one through college age along with their families, caregivers and professionals in the field. The facility also provides SIU students within that field with a valuable educational experience.
For more information about the center or Luechtefeld and Bost's visit, contact Valerie E. Boyer, assistant professor and center director, at 618/453-8262 or by email at firstname.lastname@example.com.


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For Mexico, the dialogue and collaboration with regional and international organizations strengthens its actions and policies to guarantee the promotion and protection of human rights.
In 2001, the United Nations High Commissioner for Human Rights established an office in Mexico (OHCHR-Mexico).
- The Office works in alliance with entities of the Executive, Legislative and Judicial branches, in order to develop joint working plans, local human rights programmes and legal harmonization initiatives.
Mexico promotes specific human rights topics in key UN summits and conferences, such as:
- The 2030 Agenda for Sustainable Development and the Sustainable Development Goals (SDG's).
- The United Nations Special Session on the World Drug Problem (UNGASS 2016).
- The New York Declaration adopted at the High-Level Meeting to address the large movements of refugees and migrants.
- The Global Compact for Safe, Orderly and Regular Migration (GCM).
Inter-American Human Rights System
- Mexico is one of the States of the hemisphere that most collaborates with the Inter-American Human Rights System.
- In the last three years, Mexico has hosted both the Commission and the Inter-American Court of Human Rights. In fact, Mexico is now the second member State of the hemisphere to have sponsored three extraordinary sessions of the Inter-American Court.
- Mexico is still the main contributor of Latin America and the Caribbean to the Court and the Commission. Mexico has financially supported both bodies in an uninterrupted form.
- In addition to being up-to-date on our budget commitments with the OAS, we have made voluntary contributions to the Commission and the Court, the most recent in 2017.
- During the 47th General Assembly of the OAS (Cancun, Mexico, June 2017), member States adopted by consensus the resolution presented and promoted by Mexico in order to double the resources of the Regular Fund destined for the Inter-American Human Rights organs
- The adopted resolution contemplates:
- Duplication of the budget allocated to the Inter-American organs over a period of three years, with incremental budgetary allocations of 33% per year, based on the budget approved in 2017, beginning in 2018.
- Fund increases of 26% and 49% based on the authorized budget for 2017 for the Inter-American Commission of Women (CIM) and the Inter-American Children's Institute (IIN) respectively, in order to support their activities in accordance to their strategic plans.
- Additional funding for development programs.

subset: commoncrawl


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Caution advised in scrutiny of SF officer in 2 shootings this year
The vast majority of San Francisco's 2,200 police officers will retire without ever firing a gun on duty. But the officer who killed a man this week as he allegedly stabbed a Subway sandwich worker was the same officer who wounded a man in the city's only other police shooting this year, said the public defender's office.
The back-to-back shootings by the same officer, Kenneth Cha, are rare, but experts on police use of force advised caution in scrutinizing the officer's actions.
"The mere fact that an officer is involved in two deadly force situations doesn't in itself prove anything," said Eugene O'Donnell, a professor at John Jay College of Criminal Justice in New York City and a former New York police officer. "There is no predictability of when an officer is going to have to fire their gun. You can have one improper shooting and one totally proper shooting. You want to keep your objectivity when you analyze it."
Though police officials have not identified the officer who shot 26-year-old Nicholas Flusche as he allegedly stabbed a Subway employee Wednesday on Market Street, the public defender's office said it was Cha, who wounded Sean Moore during a Jan. 6 encounter outside his Ocean View home. The public defender's office is representing Moore, who faces criminal charges.
The office and community activists have criticized the earlier shooting, saying the officers' body camera footage showed Cha and Officer Colin Patino unnecessarily escalating a tense situation with Moore, who, according to his family, has a history of schizophrenia. But police officials say Cha fired to defend himself and his partner from Moore, and they released photos showing the officers had been bloodied by Moore.
"I feel like with Sean's case, Kenneth Cha overreacted," said Deputy Public Defender Brian Pearlman. But in the Subway store shooting, he said, "I have no idea. He might have had to save someone's life, and that is the right thing to do.
He continued, "Even if it is a justified shooting that he will be cleared on, just doing that so quickly after being back on the street again is definitely concerning. It could just be bad luck, but it's hard to make a judgment without knowing the facts of the other case."
Since 2000, only three other San Francisco officers appear to have been involved in more than one shooting, according to records released by the Police Department. Most recently, Officer Nathan Chew, who was cleared of criminal charges and civil liability in the 2014 fatal shooting of Alejandro "Alex" Nieto on Bernal Hill, fatally shot 26-year-old Nicolas McWherter last year after McWherter shot and seriously wounded Officer Kevin Downs.
Ed Obayashi, an attorney and Plumas County sheriff's deputy who is an instructor at the Alameda County Regional Training Center on use-of-force investigations, said no national database tracks officers who have used deadly force in the line of duty. Therefore, there are no statistics for how often officers are involved in multiple incidents. But repeat incidents are "extremely rare," he said.
"It's extremely rare because, contrary to popular belief, the overall majority of cops never fire their weapon during their career," Obayashi said.
Oakland civil rights attorney John Burris, whose office plans to represent Moore in a potential lawsuit, said he handled a case in the 1990s involving an Oakland police officer who shot a 16-year-old high school student and then killed a homeless woman less than six months later.
"The thinking was that he wasn't emotionally suited for the work and he wasn't suited to be in Oakland in many ways," Burris said. "I haven't seen anything like that recently, and I hope that means (police departments) are exercising some restraint and are assessing the situation better and are appreciating the repercussions that flow from it. I do believe many officers are affected by the use of deadly force, whether they were right or wrong at the time. They are affected emotionally and I think and hope that that is cause for reflection."
For a variety of reasons, some officers never return to street duty after a shooting, but most do. San Francisco police Sgt. John Crudo of the internal affairs unit told The Chronicle last year that after a shooting, officers are placed on paid administrative leave for a minimum of 10 days. During that time, they meet with peer counselors and mental health counselors and receive a replacement gun.
The officers must prove themselves at the gun range as well as with a use-of-force simulator, which puts officers in a number of scenarios that require them to choose what to do and which weapon to use, if any.
A return-to-duty panel consisting of the officer's supervisors as well as homicide and internal affairs investigators convene within five business days to discuss the facts of the shooting and decide whether to recommend to the chief that the officer be allowed to return to regular duty.
Pearlman said that after the Moore shooting, he was concerned with the amount of experience Cha and Patino had. Both had spent less than a year on the street with no training supervisor, and neither had been through the department's crisis-intervention training.
But studying an officer's actions to determine if red flags exist should never be limited to shootings, law enforcement experts said. They said police departments must study other factors such as any complaints filed against officers or whether their uses of force follow a concerning pattern.
While department officials should try to learn from each shooting, O'Donnell said, they also need to be wary of making officers fearful to use force when necessary.
"You don't want to create an environment in which somebody is being stabbed and the police don't want to shoot," O'Donnell said. "That's a real issue, too."
Vivian Ho is a San Francisco Chronicle staff writer. Email: firstname.lastname@example.com Twitter: @VivianHo


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Peace to All! Below please find the official English translation of Our Lady's November 25, 2007 message to the world as provided by the Information Center of Medjugorje.
God Bless!
Steve and Ana Shawl
the Medjugorje Web
Message of November 25, 2007
"Dear children! Today, when you celebrate Christ, the King of all that is created, I desire for Him to be the King of your lives. Only through giving, little children, can you comprehend the gift of Jesus´ sacrifice on the Cross for each of you. Little children, give time to God that He may transform you and fill you with His grace, so that you may be a grace for others. For you, little children, I am a gift of grace and love, which comes from God for this peaceless world. Thank you for having responded to my call."
Our Lady of Medjugorje has appeared to six visionaries since 1981, telling us God exists, and that we need to return to a life of prayer. She has told them these will be her last apparitions on Earth.
This blog is here to help you live a the full, holy, and enriching life Our Lady says God wants us to live, by providing you with reviews of books related to the apparitions and messages.
Sunday, November 25, 2007
Medjugorje - Our Lady's November 25, 2007 Message
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British boy band One Direction were recently forced to reschedule some of the dates of the much awaited UK Arena tour for next year due to scheduling conflicts. But the fans of the band do not have to be disappointed as everything has been taken care of.
Just four of the 34 dates have been adjusted that includes shows originally set for March 24 at Newcastle Metro Arena, March 26 at Sheffield Arena, March 27 at Newcastle Metro Arena and March 29 at Glasgow SECC. One Direction is at present on tour in the US with their friends Big Time Rush.
The band will continue touring despite the exit of the rocker Zayn Malik due to a death in the family. The British band is also slated to perform at the Nickelodeon Kid's Choice Awards and will also be hosting the network's Saturday night line up leading to Big Time Rush's Big Time Movie.
So as to pay tribute their friends and to commemorate the occasion , One Direction recently covered a Big Time Rush track as well as on British English. The popular boy band will be performing in some of the biggest Arenas in the UK including Manchester's Arena.


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Park to welcome all for fun in the snow
PRESQUE ISLE, Maine — Aroostook State Park will host Winter Family Fun Day from 10 a.m. to 3 p.m. on Saturday, Feb. 23, 2019.
All are welcome to spend a winter's day at the park, where officials will have everything needed to have fun with the family outside in Aroostook County's winter wonderland.
Activities will include cross-country skiing, snowshoeing, a sliding hill, snowmobile "tote rides," bonfires and hot food.
"Have no equipment? Don't know how to use it? Don't worry; we have all of that, including expert instruction," said park director Scott Thompson. "All you need is to wear warm winter clothing and want to have fun."
The fee for adults 12-64 is $1.50; all others will be admitted free.
Lunch will be provided. For more information, call 207-768-8341.


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Alhaji Mohammed Masaba, a cleric who married over 90 wives, has died at the age of 93. It's surprising that a Man with so much wives lived this long oh…how did he do it?
The controversial husband of 97 wives died on Saturday afternoon at his residence in Efu Nakodi after a brief illness. His death was confirmed by his Personal Assistant, Alhaji Mutairu Salawudeen Bello on Saturday afternoon to journalists.
According to him,
"With gratitude to Allah (SWT), we announce the death of Alhaji Mohammed Abubakar Bello this afternoon. He was aged 93 years. He died after a brief illness.
"The Janiza prayer and interment is slated for tomorrow (Sunday) by 10am."
The late cleric's aide added that the preacher had foretold his death and was prepared for it. Bello
"Baba had told us that his time was up.
"He told me personally during his last moment that he has completed his divine assignment and was ready to meet his creator.
"He told us to remain dedicated to the cause of Islam and urged us all not to deviate from all his teachings on righteousness, piety and total submission to the will of Allah.
"He warned us to shun adultery, but that we should marry our women because it is 'Hallal' before Allah."


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This week I started a new day job (and welcome to everyone visiting from Value Hearing!).
One of the things I've been doing is creating client avatars. In marketing speak a client avatar is a distillation of a 'typical' client – their demographic profile, creating a backstory which leads to their moment of decision.
It's not unlike creating compelling characters in a novel.
The way I look at it is that all of us of human beings are like concentric circles.
The outer ring
The outermost circle is the one we project out to the world. It is a very low resolution version of who we are as people.
I hesitate to use the word stereotype because of its negative connotations.
Perhaps it is better described as the series of common and familiar elements which helps us decisions on how to interact with a stranger on how they present.
For example, if someone is wearing a police uniform, that tells us how we should relate to them. If they instruct us to do something, we do it.
A person wearing a white coat with a stethoscope around their neck is likely to be a doctor.
In fact, it's become a trope – The Clipboard of Authority.
That comes from a line from Michael Keaton in The Paper: "A clipboard and a confident wave will get you into any building in the country."
Another example would be the NPC, the non-player characters in video games. They are simply there to add colour or give basic instructions, but they are not someone we spend a lot of time with and have little influence on the game.
Unfortunately a lot of supporting characters in novels can be like that. They serve the purpose of steering our protagonist down one road or another and resort to stereotypes to do it.
Examples abound in entertainment – the heroine's waspish gay best friend; the sassy African-American woman; the weak-chinned vicar; judgemental church lady; the technologically savvy children; the dopey dad.
Now, I realise that the thing which drives a novel (at least in popular fiction) is the story. Literary fiction can be character studies, but popular (or genre) fiction has to show the characters doing something.
Having a cast of characters is great and they have to be there to serve a purpose (even if it is simply to add a bit of colour).
The second ring
But I recommend going a little deeper in these characterisations and that's where the tools to great customer avatars in useful.
This is the second concentric ring. This provides motivation of these characters that is quite outside any interactions your main protagonists (your hero and heroine) have with them.
If the traffic cop is surly, ask why? Is he at the end of a long shift? Has he just returned from doing a death knock? Has he broken up with his wife?
Not that you have to create a family tree for him or even include that level of detail in your story, but what it does is give you the opportunity to do more go skin deep.
Perhaps the cop has dark circles under his eyes. Maybe the protagonist notices that there is a pale band on his hand where a wedding band used to be.
Just go that little bit deeper and make sure they are as least as motivated as your main characters.
The final ring
The last ring in the concentric circle is the full POV (point of view), that means we not only know what motivates them and infer why, that inner circle is also the inner dialogue will actually tell us why.
This is actually the most intimate form of relationship. Done right, we actually have a visceral reaction to the words on the page.
The compelling characters are fully fleshed out human beings, we feel we could see them in the street and know them. And great authors can make you feel that way about all of the important characters, even those you're not supposed to like
In the world of romance novels, that tends to be limited to the hero, heroine and villain.
That little bit of extra detail to those all important secondary characters creates a universe your readers will find compelling.
Excerpt Live And Let Spy
The Angler's Arms barn was dressed for the troyl. Swags of greenery festooned the walls, studded with posies of wildflowers of white, yellow and shades of pink. Outside, a pig was roasting on a spit, the smell of which was already making Adam famished.
He had been looking forward to this event all week. Frankly, it was exhausting to be on alert all the time; mindful of every action in case one is watched, and watching everyone else around you, looking for a hint they might be a spy and a traitor.
But tonight was all about the simple pleasure of a country dance among friends, where he could give himself in to the moment without reserve. It would be like furlough.
He settled himself on the edge of an unopened barrel by a door near the back of the inn.
He noticed Will lurking around the back of the kitchen. They shared a nod. No doubt the young man was waiting for his mother and one of her maids to leave in order to help himself to one of the fairings, a crisp, sweet and spicy ginger biscuit that had been left out to cool.
A moment later, the lad lunged out of sight a moment before emerging with two of the delicious morsels. Adam was surprised to find himself presented with one half of the spoils.
The young man grinned at him.
"If Mamb catches me, I'll tell her you were the pilferer, then I won't get a clip around the ear for it."
Adam laughed. "Don't be so sure your mother won't give me a pinch for it either, so we'd better eat these now and leave no evidence."
"William Bartholomew Trellow!"
The young man jumped and the winced at the sound of his full name being yelled in top voice by his mother.
"Make yourself scarce, Will. I'll try to delay her for as long as possible," Adam said with mock urgency.
Will didn't require a second invitation. Adam chuckled as he watched the large young man sprint down the length of the barn and nearly losing his balance skidding on loose gravel as he rounded the corner.
He heard the sound of a woman's footsteps and was conscious of the fairing in his hand, the smell of warm ginger reaching his nose. For a half-moment he considered taking off after Will.
Instead he turned, waiting to face his punishment like a man. But instead of seeing the thunderous face of Polly Trellow, he saw a vision of beauty instead. He immediately got to his feet.
Olivia smiled at him nervously and lightly brushed the back of her hand down the skirt of her cream dress embroidered with flowers of light blue, pink and green.
Adam held up the filched fairing. "Share this with me?"
Olivia shook her head. "Miss Lydia made a gift of this dress when she left and this is the first time I've worn anything so fine. I'm afraid I'll spill something and spoil it."
He took her hand, raised it to his lips, and kissed it. He loved the way her lips parted when he did that. The dress was flattering to be sure, he considered, but it was still only a dress.
"It is only the wearer who can make a piece of fabric and thread look so fine," he told her and she blushed.
Live and Let Spy is book 1 in The King's Rogues series.


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Live cattle futures were mixed after Tuesday's close, the April contracts were nudged 2 cents in the black while Feb and Dec futures remained lower. Feeder cattle futures were also mixed at the closing bell, as they couldn't hang on to midday gains. The 12/02 CME Feeder Cattle index was $145.03 after gaining 6 cents. Official Brazilian exports show beef shipments of 155,600 MT for Nov, which is down from October shipments, but they exported 19.23% more than November a year ago. Afternoon boxed beef prices were lower but tighten the spread to $17.84. Choice boxes were $2.46 lower, but select boxes were $0.67 lower. Tomorrow's FCE online cattle auction will host 1,189 head, 827 from NE 230 head from KS and TX offering 142 head. Cash cattle activity has been light recently, the USDA has reported 80% choice dressed sales of $187.00, and live from sales $116 - $119 throughout the week. USDA estimated the weekly total FI cattle slaughter at 240,000 head through the beginning of the week. Monday's estimate was revised to 120,000.
Dec 19 Cattle closed at $120.550, down $0.200,
Feb 20 Cattle closed at $125.650, down $0.150,
Apr 20 Cattle closed at $126.225, up $0.025,
Jan 20 Feeder Cattle closed at $142.375, up $0.225
Mar 20 Feeder Cattle closed at $142.925, down $0.100
Apr 20 Feeder Cattle closed at $144.950, down $0.025
---provided by Brugler Marketing & Management


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If you dig wood, hard work, and sweating the small stuff then Daneson wants to hear from you.
Get in touch - even if you don't see a role below that blows your hair back.
Please send in proposals and CV to jobs @ daneson.com.
Working under the supervision of the Senior Art Director, Brand Marketing you will provide the art direction, creative development, and design execution of creative briefs.
The position is hands-on in terms of design and execution.
A strong understanding of social media, photography, video, web, and print is key.
You will be creating digital, content, ads, websites, email, and more.
You will have a track record for leading ideas from rough draft to completed project.
Ideally, you have experience in CPG.
If you have experience creating affiliate campaigns and are looking for a side hustle then please get in touch. Daneson can be found amongst the top fashion, gourmet, and hospitality retailers in the world and your role would be to build on this success by lining up affiliate partnerships.
If you have experience running Facebook campaigns.
If you nerd out hard on numbers and are fastidious about figuring out what does, and does not work.
And if you have a smattering of experience other digital marketing channels.
Then we want to hear from you.


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New Research In
Physical Sciences
Social Sciences
Featured Portals
Articles by Topic
Biological Sciences
Featured Portals
Articles by Topic
- Agricultural Sciences
- Anthropology
- Applied Biological Sciences
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- Genetics
- Immunology and Inflammation
- Medical Sciences
- Microbiology
- Neuroscience
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- Physiology
- Plant Biology
- Population Biology
- Psychological and Cognitive Sciences
- Sustainability Science
- Systems Biology
Investigating stem cells in human colon by using methylation patterns
Edited by Bert Vogelstein, Johns Hopkins Oncology Center, Baltimore, MD, and approved July 12, 2001 (received for review May 7, 2001)
Related Article
The stem cells that maintain human colon crypts are poorly characterized. To better determine stem cell numbers and how they divide, epigenetic patterns were used as cell fate markers. Methylation exhibits somatic inheritance and random changes that potentially record lifelong stem cell division histories as binary strings or tags in adjacent CpG sites. Methylation tag contents of individual crypts were sampled with bisulfite sequencing at three presumably neutral loci. Methylation increased with aging but varied between crypts and was mosaic within single crypts. Some crypts appeared to be quasi-clonal as they contained more unique tags than expected if crypts were maintained by single immortal stem cells. The complex epigenetic patterns were more consistent with a crypt niche model wherein multiple stem cells were present and replaced through periodic symmetric divisions. Methylation tags provide evidence that normal human crypts are long-lived, accumulate random methylation errors, and contain multiple stem cells that go through "bottlenecks" during life.
Most of the information on intestinal stem cells has been inferred in mice by using histologic markers. For example, crypts at chimeric patch boundaries are initially polyclonal but subsequently display only a single marker by 2 weeks after birth (1). This process implies that crypts are monoclonal because they are derived from single progenitors. Mutagenesis can also induce crypt heterogeneity that subsequently declines as markers become uniformly present or absent. These observations suggest that crypts are maintained by small numbers (between 1–40) of variously defined stem cells (2, 3). This stem cell architecture may protect against neoplasia as most non-stem cells differentiate and die within a week (4).
Less is known about human crypts. Human and murine dynamics may be similar as some patients demonstrate mutant crypt frequencies consistent with those measured in the mouse (5). Human studies are difficult because strategies that directly mark cell fates, such as aggregation chimeras and mutagenesis, are impractical in human beings. Longer lifetimes and larger human crypts may pose unique challenges for crypt maintenance and prevention of neoplasia.
Alternatives to experimentally created visible markers are endogenous sequences that become polymorphic with aging. Related cells should share such sequence polymorphisms. Although low mutation frequencies hinder this strategy, recent studies demonstrate changes in methylation of some CpG islands with aging in normal human colon (6, 7). Methylation exhibits somatic inheritance and random site autonomous changes (8, 9) that accumulate more frequently than mutations (10). Methylation can modulate transcription and chromatin structure (11, 12), but some changes may reflect random errors, especially at CpG sites of unexpressed genes. Such age-related errors can potentially encode binary (methylated vs. unmethylated) information strings in adjacent CpG sites, creating tags that can be read with bisulfite sequencing. Although human colon crypts presumably originate from single progenitors (1–3), they could become quasi-clonal (contain multiple tags) depending on epigenetic and stem cell dynamics. As an alternative to histologic examination, it may be possible to recreate crypt histories by comparing epigenetic tags within and between crypts.
Isolation of Single Crypts.
Individual crypts (>95% epithelial cells) were isolated (13) from ≈1–2 cm2 normal patches of 10 fresh colectomy specimens (Table 1). DNA was extracted in a 10-μl solution (100 mM Tris⋅HCl/4 mM EDTA, pH 8.0/200 μg/ml of proteinase K) for 4 hours at 56°C, followed by boiling for 5 min. Cytosine bases were converted by bisulfite treatment (14), using an agarose bead method (15). Agarose bead sizes were 20–30 μl. DNA was also extracted from microdissected epithelial regions of four paraffin-embedded colons to estimate methylation of younger individuals.
Methylation Analysis.
Patterns were analyzed for three loci (Fig. 1): CpG islands in myogenic factor 3 (MYOD1; GenBank accession no. AF027148; nucleotides 9,432–9,557), cardiac-specific homeobox (CSX; GenBank accession no. NM004387; nucleotides 1,271–1,377), and an X-chromosome CpG-rich region in biglycan (BGN; GenBank accession no. M65151; nucleotides 519–648). Age-related methylation has been demonstrated for MYOD1 (6) and CSX [Mint 23 (7)]. BGN is a small cellular or pericellular matrix proteoglycan primarily expressed in connective tissue (16). The serial analysis of gene expression database (www.ncbi.nlm.nih.gov/SAGE) failed to yield tags specific for these loci in normal or neoplastic colon tissues.
An average of 7.5 crypts (range 7–9) per patient were examined for each locus. Primers amplified only bisulfite-converted DNA and were (5′-3′) MYOD1: F-GGAGGGGATTTTTAATTTGGGT and R-AAACCCAATCCTTCTTCCCTAA; CSX: F-GGAGATTTAGGAATTTTTTTTGTTTT and R-ACACCAAACTACAAAATCACTCATTA; and BGN: F1-TTTAGGAGTGAGTAGTTGTTTTTGGTT, F2-TAGGAGTGAGTAGTTGTTTTCGGTT, and R-ACCAACCCTACCTAAAAAAAAAAAA.
Two forward BGN primers were used to avoid bias caused by an internal CpG site. Approximately half the bead was amplified with 42 PCR cycles (60°C annealing temperature) in 4 independent reactions by using AmpliTaq Gold polymerase (Perkin–Elmer), 4 mM MgCl2, and 1 μM of each primer in 50-μl volumes. PCR products were combined, and the mixtures were cloned (Topo-TA cloning kit, Invitrogen). At least 5 clones or molecules per locus (averages of 7.5 for MYOD1, 7.8 for CSX, and 7.2 for BGN) per crypt were sequenced. Sequences with incomplete bisulfite conversion (Cs at non-CpG sites) were rare (<2% of all molecules) and excluded from analysis. Mutations at non-CpG sites were rare (<0.0005 per nucleotide).
To examine whether sampling could detect crypt diversity, seven crypts were combined and then subdivided into seven "mixed" crypt extracts. Six mixed crypts from three patients were analyzed at CSX and MYOD1 as described previously. Diversity (see below) of the mixed crypts should be greater than single crypts and equivalent to the diversity calculated from the molecules of all individual crypts. Distances of the mixed crypts were on average 1.4 times greater than the average single crypt, and 89% of the average distances of all molecules from the individual patients, which was consistent with an ability to detect heterogeneous crypts with our sampling scheme.
A number of statistics that describe methylation patterns were used. Percent methylation is determined by averaging sites from all molecules from each crypt. The number of unique tags is the number of different tags found in each crypt. Average percent crypt methylation and unique tag number are the means of crypt values. Diversity (or epigenetic distance between molecules) between a pair of tags is calculated as the number of differences at each CpG site. For example, if a methylated site is coded as a 1 and an unmethylated site is coded as a 0, the distance between 01001 and 00101 is 2. Maximum distances between two molecules are 5, 8, and 9 for MYOD1, CSX, and BGN, respectively. Intracrypt distance is the average difference of all possible pairs of molecules within a crypt. Average intracrypt distance is the mean of all intracrypt distances. Intercrypt distance is the average difference of all possible pairs of molecules chosen from different crypts. A polymorphism eliminated one BGN CpG site for patient M and calculations were based on eight sites.
Drift can record relationships between cells. More closely related cells share alterations whereas less closely related cells have greater differences. Sequence differences within crypts reflect numbers of stem cells and how they divide (Fig. 2). Crypts may be maintained by small numbers of immortal stem cells or by niches where stem cells undergo random loss with replacement. Differences between these scenarios may be small because each cell within a stem cell niche may replace itself like an immortal stem cell >90% of the time. However, random stem cell loss with replacement eventually leads to sequential "bottlenecks" where all "stem" cells within the niche are related to a new most recent common ancestor. Bottlenecks never occur with immortal stem cells. Therefore, over decades, crypts maintained by multiple immortal stem cells will contain increasingly diverse sequences with numbers of unique sequences proportional to numbers of stem cells. For a niche, crypt sequences will be more closely related, and numbers of unique sequences within each crypt will be variable and depend on the time since a last common crypt ancestor.
To apply this molecular bookkeeping strategy to epigenetic patterns requires evidence of drift. Crypts similar at birth [initially unmethylated (6, 7)] should become different over decades. Unlike histologic markers limited to two states (present or absent), multiple methylation tags are possible. When there are N CpG sites, there are 2N possible tags, or 32, 256, and 512 possible unique tags for MYOD1, CSX, and BGN. Methylation at the three examined loci (Fig. 1) is unlikely to be under selection because the loci are not significantly expressed in normal or neoplastic colon. Single crypts were sampled from 10 patients, and tags were read by sequencing cloned PCR products after bisulfite treatment (Table 1). Multiple unique tags were present in some crypts, and tags were often different between crypts, which is consistent with random tag drift (Fig. 3). For example, none of the methylated BGN tags were the same between crypts in patient F.
Although tags were different within crypts, the tags were related. Fluctuations with sample sizes were plotted (Fig. 4). Percent methylation and epigenetic distances were relatively stable after five molecules were examined from a crypt. Small numbers of tags may accurately sample these values when crypt cells are related. Small numbers of molecules did not fully sample crypt diversity because new tags could be found even after 16 molecules. Many unique crypt tags suggest multiple stem cells, which is not unexpected because murine crypts may contain as many as 40 stem cells (3).
Quasi-Clonal Human Crypts.
Methylation revealed differences between morphologically identical crypts. Average numbers of unique tags per crypt were similar between individuals (2.6 for MYOD1, 3.1 for CSX, and 2.4 for male patients at the X-chromosome locus BGN). More consistent with a niche scenario, numbers of unique tags per crypt were variable (Fig. 5A). Maximum observed numbers of unique tags were 8, 9, and 7 (of 11, 18, and 8 sampled molecules), respectively, per crypt. These numbers exceed the one or two tags expected for a strictly monoclonal population. Most normal human crypts are quasi-clonal with respect to methylation.
Epigenetic variation could be generated with multiple long-lived stem cells or in non-stem cells during crypt epithelial maturation. To determine whether new tags are frequently created during the ≈1 week required for maturation (2, 3, 17), patterns were compared between seven bisected crypt regions. Consistent with stem cell origins for most methylation patterns, upper and lower crypt portions contained similar tags, although frequencies were sometimes different (Fig. 3). Simulations were also consistent with relatively few methylation changes during epithelial maturation because the estimated methylation error rate was 2 × 10−5 per division (see below).
Mosaic Methylation Increases with Aging.
Average crypt methylation increased with aging (Fig. 5B). This age-related process was complex because methylation varied between crypts within individuals. Crypts with greater than 40% methylation were present in younger individuals (<50 years old), and crypts with less than 5% methylation were present in older individuals (>60 years old). Normal human colon becomes a methylation mosaic.
Methylation frequencies were unequal between CpG sites (Fig. 1). These differences may be the result of stochastic variation and a tendency for some sites to become methylated. Overall, methylation resembled a random process (8, 9). There was also variability between tags and CpG islands within a crypt. Hyper- and hypomethylated CSX or MYOD1 tags were present in some crypts (Fig. 3). Percent methylation between MYOD1 and CSX did not correlate within single crypts (Fig. 6). Methylation of one allele does not predict the status of the other allele or another CpG island.
Aging of Colon but Not Crypts.
Methylation patterns differed between crypts but were more similar within crypts (Fig. 3). Distances between crypts and within crypts were variable, with greater intercrypt compared with intracrypt distances (Fig. 5C). Diversity of the colon is greater than individual crypts.
Crypt Dynamics.
To understand further the experimental observations, crypts were simulated starting with unmethylated tags, a methylation error rate of 2 × 10−5 per CpG site per division, and one division per day. In our model, a crypt always has N stem cells, as well as a number of non-stem cells. Each stem cell produces two cells, of which either 0, 1, or 2 are stem cells with probabilities q, p, and q, respectively (where p + 2q = 1), constrained to ensure a total of N stem cells in the next generation (18). Non-stem cells always produce non-stem cell offspring (see supporting information, which is published on the PNAS web site, www.pnas.org). There are two models we wish to contrast. The first, which has p = 1, corresponds to immortal stem cells. The second, for which 0 ≤ p < 1, corresponds to a stem cell niche architecture (19).
The immortal model with N = 2 fits average crypt values satisfactorily, whereas the stem cell niche model fits well with N of at least 4 (Fig. 8, which is published as supporting information on the PNAS web site). However, the two models predict very different behaviors for the variance of the average crypt values. Variances in numbers of unique tags per crypt within individuals were always greater than the simulated 97.5th percentile for immortal stem cells, but most were between the simulated 2.5th and 97.5th percentiles for crypt niche models (Table 2). The size of this niche could not be determined because from 4 to 512 cell niches with p = 0.98 to p = 0.75, respectively, were consistent with the data (Fig. 9, which is published as supporting information on the PNAS web site). Identical crypt niche and epigenetic parameters applied to all three loci examined, largely recapitulating both average values and scatter of individual crypts (Figs. 5 and 9).
A crypt at different times may have distantly or closely related cells because of random stem cell loss and replacement. For a 64-stem cell niche [assuming the 95% asymmetrical division estimated for murine crypts (20); Fig. 7], the most recent common ancestral stem cell of the stem cells present at a given time is on average ≈3,000 divisions ago (with a 95% interquantile range of 1,000–7,000). A bottleneck recurs on average every 8.2 years. If 1 of 64 crypt stem cells was mutated, the mean time for this mutant stem cell to become completely dominant or extinct in our model was 220 days (with a 95% interquantile range of 2–1,900 days), which is consistent with 1-year clonal stabilization times inferred from human crypt heterogeneity after irradiation (5).
Murine crypt architecture is inferred from intervals in which visibly mixed crypts become homogeneous (1–3). This approach can also be applied to humans by comparing epigenetic tags within and between morphologically identical crypts. Methylation tags allow fate-mapping because their patterns are somatically inherited (10) but drift within a lifetime to become polymorphic. Heterogeneous methylation patterns have been observed in other adult human tissues (21), tumors (9, 22), and within aberrant colon crypts (23). Changes in human tags with aging can potentially yield fate maps for a variety of adult tissues, extending observations decades beyond those possible in mice.
Methylation patterns were extremely variable or mosaic between crypts within an individual. Percent methylation did not correlate between CpG islands within single crypts. Simulations suggest methylation with aging (6) is a consequence of stochastic errors that begin from birth rather than loss of an ability to maintain unmethylated CpG sites. The estimated error rate of 2 × 10−5 per CpG site per division in human crypts is lower than observed in tissue culture (8, 24). However, culture may effect methylation patterns, and some measurements occurred after experimental demethylation. This estimated epigenetic error rate is about 4 orders of magnitude higher than human DNA replication [10−9 per base (25)]. Drift would inherently increase average methylation with aging because the loci, like most CpG islands (26), were initially unmethylated. Fidelity may differ at other CpG sites because of selection or different mechanisms of methylation.
Despite clonal origins and small sizes (≈2,000 cells), many normal human colon crypts contained multiple unique tags and were quasi-clonal. Multiple crypt tags likely reflect multiple long-lived stem cells. Stem cell definitions are variable but all include self-renewal through asymmetric divisions. The current simulations define stem cells through self-renewal and infer "stemness" as a property of a compartment or niche (19) rather than an intrinsic cell-determined fate. Variability of unique tag numbers between crypts is greater than predicted for immortal stem cells and more consistent with the stochastic properties of a stem cell niche. This crypt niche is functionally similar to the germ cell niche recently characterized in Drosophila (27–29). The exact number of cells in this niche is uncertain and may vary between crypts and individuals. Similar to mice (3), as many as 10% of human crypt cells may exhibit stem cell-like properties.
Symmetric divisions allow stem cell removal or expansion that effectively oscillates and limits intracrypt diversity. Somatic losses of stem cells inferred after mutagenesis appear to occur in unperturbed normal human crypts. Bottlenecks or reduction to a new single most recent common crypt progenitor are predicted to recur several times during life, superficially resembling the clonal succession of tumor progression (30). Stem cell multiplicity may help ensure crypt integrity and survival, leading to mosaic methylation between crypts.
Other models could account for mosaic methylation, and many assumptions cannot be directly tested and may differ between crypts or individuals. However, it is currently impossible to isolate or identify crypt stem cells, and methylation tags allow noninvasive systematic studies of normal human crypts. Crypt histories seem to be at least partially recorded in tags as a simple model consistent with murine studies that was able to organize complex methylation patterns from different patients.
Methylation tags provide evidence that normal human crypts are long-lived, contain multiple stem cells, undergo continuous bottlenecks, and accumulate random methylation errors at some loci. Tags reveal two ongoing counteracting but otherwise invisible forces shaping crypt diversity—drift and extinction. Drift is difficult to document because mutations are rare in normal human tissues (31). Labile epigenetic markers readily reveal differences within and between otherwise morphologically identical crypts. A stem cell niche architecture minimizes the risk of cancer (4), and periodic stem cell losses would eliminate some alterations that occur with aging. These dynamics may be important from a perspective of early multistep tumorigenesis because global hypomethylation (32) or mutagenesis before crypts become monoclonal (33) and respectively decrease or increase adenomas in Min mice. Methylation tags or other sufficiently labile molecular clocks (34) provide opportunities to investigate otherwise invisible cell fates during human somatic evolution.
This work was supported by National Institutes of Health Grant CA70858 (to D.S.) and by National Science Foundation Grant BIR95-04393 (to S.T.).
↵† Present address: Department of Pathology and Molecular Diagnostics, Aichi Cancer Center, Nagoya 464-8681, Japan.
↵§ To whom reprint requests should be addressed at: University of Southern California School of Medicine, 1200 North State Street, Box 736, Los Angeles, CA 90033. E-mail: dshibata{at}hsc.usc.edu.
This paper was submitted directly (Track II) to the PNAS office.
See commentary on page 10519.
- myogenic factor 3;
- cardiac-specific homeobox;
- biglycan
- Received May 7, 2001.
- Copyright © 2001, The National Academy of Sciences
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- Shibata D


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There is a reason being rich is not a sin, but envy is a sin.
Socialism is Here to Stay
by Jeff Thomas
"[T]he government has to take resources from someone before it can dole them out to others. This act of taking destroys an economy. The more you take from the productive members of society, the less productive they become. That's the primary lesson of the history of socialism."
The above quote is from Porter Stansbury – from his book, America 2020. It states a concept that I've described for years, but Porter states it more succinctly than I ever have. In particular, it negates the argument by many "progressives" that, even if they don't recommend full-on socialism, they believe in getting "just the right mix" of socialism and capitalism to create the ideal system.
Unfortunately, as viable as this concept may sound, even moderate socialistic national policies result in moderate deterioration of the system. It's not unlike being "just a little addicted" to heroin.
It may be argued that, "That's different. With heroin, the addict will always end up wanting more and he'll become even more dependent." Exactly so – and that's unquestionably true for socialism as well. Once the concept of "free stuff" is part of a nation's governing system, the desire for more free stuff will inexorably rise.
And, of course, historically, we have seen that governments always step up to the plate whenever the demand for more free stuff is suggested. But why should this be so?
Wouldn't a more conservative (less) government be less likely to proffer entitlements than a more liberal (more) government? Isn't that government best that governs least as Henry David Thoreau wrote? Wouldn't a free market be better than a centrally planned economy? Yes, but governments trend more liberal (more government) over time.
In a free-market society, a government is not especially necessary. It may be needed to defend the country if it's invaded, or, arguably, it may be useful in creating a national currency, building national highways, etc. (But even these needs may be argued.)
A free-market society is beneficial, as it creates prosperity. It enriches the population with both money, goods and services. It also rewards those who are the most productive. However, it does tend to leave behind those that are less productive, and here's where political leaders find their opportunity to cash in.
Let's say we have a country that's made up of five voters, with their respective net worths as follows:
Voter A: $1
Voter B: $10
Voter C: $100
Voter D: $1,000
Voter E: $10,000
If I were running for office and declared that no one should own more than $10, I would not be elected, as most voters would quite rightfully regard me as a threat. But if I were to declare that "the greedy rich" have too much money and should be required to "give some back," I might get all voters except Voter E to vote for me.
Why should this be so? Because no one thinks of himself as being amongst "the greedy rich". For the man who is worth $1,000, the greedy rich are those who are worth $10,000 or more. But, likewise, the man worth $100 thinks of the greedy rich as those worth $1000 or more. Human nature dictates that we don't see ourselves as greedy, so it's not too difficult for politicians to convince us that those who have more than us are greedy. Further, once we're convinced of this, it's not too difficult to fool us into believing that the greedy rich have, in some way achieved this wealth by swindling us out of it. And, now that you mention it, yes, we would like to have some of it back, thank you.
So, any population becomes an easy target for leaders who promise to take from the rich and "give back" to the less rich, like a modern-day Robin Hood. But what of that claim that "just the right mix" of socialism could take some away from the rich, but leave prosperity intact? Well, here's why that has never happened and will never happen in any country…
Modern political leaders do not exist to serve the populace, they exist to feed off of and dominate them. We do not have any Washingtons or Jeffersons today. But they cannot feed off of the populace without the wealth of the electorate passing through their hands. The more of the electorate's wealth passes through their hands, the greater the amount that can be skimmed off to both enrich themselves and increase their power.
And so, it's the nature of governments to seek to increase the size of government annually (requiring ever-more revenue to pass through their hands) and to take an ever-greater part in the hands-on distribution of the nation's wealth. All governments will do all they can to grow themselves, as it's very much in their interest to do so. Governments will continually pursue a greater level of socialistic policies.
So, where does that leave the individual voter?
The choice, really, is whether the individual is living in a jurisdiction where the government has already become so socialistic that he's a net loser (California, New York, Illinois…), rather than a net recipient. Beyond this point, his future can only be on a downward trajectory.
This is a most unpleasant conclusion to come to grips with, as it informs the individual not only of his current situation, but the rest of his life. In standing back and observing his entire future from a greater vantage point, he realises that, increasingly, he will be beating his head against the wall if he remains where he is.
Those who internationalise, do so with the understanding that, if they choose one country because it's the most ideal to do banking in and choose another because it's the most productive to invest in, they will prosper. At some point, they additionally realise that it's also beneficial to apply that logic to their choice of country of residence.
Throughout the life of anyone who advances himself, there's a tendency to change neighbourhoods from time to time, to attain a better quality of life. Yet, most people drop this logic as soon as they reach the borders of the country they were born in. In truth, the decision to move beyond national borders to choose a neighbourhood – one where the system has not deteriorated to the point that it's dramatically usurping the wealth of the individual – is not such a great leap. In truth, it's relatively easy to do.
In much of the former "free world", socialism is here to stay, but the individual citizen needn't be. He may vote with his feet and move to a better neighbourhood.


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Northwest Austin
Neighborhood Scores
Avg Rent
Public Transportation
What It's Known For
Looking to live in Northwest Austin?
If you're looking for a cozy apartment in Northwest Austin, we don't blame you. This neighborhood is absolutely beautiful. Living in this area, you will be right in the middle of the gorgeous Hill Country — and the views are truly amazing. Northwest Austin is typically a quieter area in Austin, and a good distance away from the bustling big city. This is a very residential spot within the city that's perfect for apartment hunters searching for somewhere peaceful to lay their heads.
This area has an abundance of things to do outdoors! One of our favorite places to explore is Mount Bonnell. This prominent lookout alongside Lake Austin has been a popular attraction since the 1850s. As Austin City's highest point, at 785 feet, Mount Bonnell lets you enjoy breathtaking views of Downtown Austin and the surrounding area. This vista is by far the best place to enjoy panoramic views of the city. Hiking to the 360 Bridge Overlook is also something that every Austinite should do, and the views are most definitely Snapchat and Instagram worthy.
In Northwest Austin, it's only a short drive to Lake Travis, the Austin Aquarium, and shopping and dining at the Domain! Apartments here are usually pretty affordable, with lots of green space and trees around that make for beautiful views.
Austin offers a number of really great neighborhoods, and our Northwest Austin apartments give you the chance to live in one of the best. Plus, properties here come with awesome amenities, including parking garages, swimming pools and more.
Finding the Perfect Apartment in Northwest Austin
Whether you're a working professional who needs access to downtown or you're a recent college grad just starting your career, Smart City Locating is here to help you find that perfect place in Northwest Austin. Finding great apartments can be a hassle, with sifting through endless websites or visiting one place after another only to come up empty. The agents at Smart City are willing and waiting to do all the legwork for you.
Our team believes that with a little bit of old-fashioned hard work, we can help each and every one of our clients find a kickass apartment they'll be proud to call home. All you have to do is lay out your wishlist — all the things you really want out of your next Northwest Austin apartment — and then we go to work. We personalize each and every search based on your individualized wants and needs.
Whether you search the Smart City site or call or text us, we provide all of our services for free. You heard right — free — as in you don't pay us a cent. What's the catch? There is none. Once you find your apartment and list us as your reference, properties pay us from their advertising budget. They get a great new tenant, you get the place you've always wanted in Northwest Austin, and we pay our bills this month. Everybody wins!
If you're ready to start, check out our Northwest Austin apartments below to discover the great deals our Austin apartment locators have found for you. Don't see anything you like just yet? No problem. Fill out our online form or give us a call or text. We're ready to go to work finding you the apartment of your dreams.


text: Happy New Year, Science Squad families! We had a lot of fun planned for the Friday before the break, so we'll be extending the holiday festivities into the New Year! On Tuesday, we will have our HOLIDAY WORKSHOP! We will make and wrap gifts for family and friends first thing in the morning, until 9:00 or so. What a nice way to start the New Year - filling buckets and spreading joy by giving gifts to people we care about! ALSO - Students may wear PJs (or any cozy, warm clothes) on Tuesday, and we will watch a short movie in the afternoon. We had reached our dojo goal in December, so we earned this reward for good behavior! On Wednesday we will get back to the business of reestablishing our learning routines! 😉


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Monthly Archives:
September 2011
Chris Nieratko for A.Skate!
Posted on Friday, September 16th, 2011
Chris Nieratko for A.Skate from Ben Duffy Presents on Vimeo.
Help A.Skate win Pepsi Grant
Posted on Friday, September 16th, 2011
Help Faith by helping A.Skate win a 50k grant from Pepsi. Bring Pepsi powervote caps to Faith and receive a 10% discount on your purchases. Go to www.askate.org for more info on this contest and how to win more prizes … Continue reading
Christian Kerr…American in Anshan
Posted on Tuesday, September 13th, 2011
Alabama native and Faith Skate Supply homie, Christian Kerr has recently relocated his endeavors to Anshan, China. Boldly, he is entering and embracing a handful of new acquaintances in culture, government, and even new time zones! Christian will be teaching … Continue reading
James Hardy gone Wild
Posted on Tuesday, September 13th, 2011
What would you do if an injury took skateboarding away from you when you wanted it more then ever….You'd probably lose your mind while you were forced to wait it out…..James Hardy went Wild.


text:
Wearing a glamorous satin gown, Ball Gown Barbie® doll makes an unforgettable entrance. A full skirt, sheer black sleeves and a beautifully tailored, off-the-shoulder bodice define the brilliant turquoise gown. A striking black belt accents her waist and opera length gloves adorn her arms. Classic underpinnings include a black tulle petticoat with satin piping and hosiery. A dazzling rhinestone brooch and earrings add the final sophisticated touches.
Barbie Fashion Model Collection


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City of Keller, TXHome Menu
Frequently Asked Questions
How can I find out if someone is in the Keller Regional Detention Facility?
- on-line search
- Telephone: 817-743-4770
- In Person: Contact jail staff from the jail lobby located at 330 Rufe Snow Dr., Keller, TX 76248
Where is the Keller Regional Detention Facility?
The jail is located at 330 Rufe Snow Drive, Keller, TX 76248.
When is the jail open?
The jail is open 24 hours a day, 7 days a week.
What type of facility is the Keller Regional Detention Facility?
The jail is a holding facility which serves the Cities of Keller, Westlake, Southlake, Colleyville, and Roanoke. Prisoners are generally confined for less than 72 hours before being released or transferred to another facility.
Where is the county jail?
Tarrant County Corrections Center, 100 N. Lamar Street, Fort Worth, TX 76102, (817)884-3116.
Denton County Jail, 127 N Woodrow Lane, Denton, TX 76205, (940)349-1700.
How can a prisoner in the Keller Regional Detention Facility be released?
Inmate bond amounts can be viewed online. Inmates may be released by paying bond amounts in full. Payment options include:
- Credit card:
- Online
- within our jail lobby located at 330 Rufe Snow Drive, Keller, TX 76248.
- Cash: (exact amounts per charge ONLY as no change can be given)
- within our jail lobby located at 330 Rufe Snow Drive, Keller, TX 76248.
- Bond Desk, Tarrant County Corrections Center, 100 N Lamar, Fort Worth, TX (817) 884-3116.
- Bail Bond Company (not commonly used for Class C misdemeanor charges):
- Arrangements may be made with a Tarrant County licensed and bonded Bail Bond Company. Several licensed and bonded companies can be found in the phone book or on the internet.
- Pre-Trial Release:
- Is a personal bond or promise to appear. Tarrant County Pre-Trial Release personnel will gather information about the prisoner in order to determine eligibility. For more details, please contact the Tarrant County Pre-Trial Release Services at (817) 884-1465. Keller jail employees cannot determine if a prisoner is eligible for this program.
Inmates may also be released by making arrangements with an attorney. Inmates with Class C misdemeanor charges may be release with applicable time-served or jail credit.
What is "jail credit"?
The Cities of Keller, Westlake, Southlake, Colleyville, and Roanoke assign a monetary value for the time an inmate spends in jail for Class C misdemeanor tickets or warrant. A municipal judge determines jail credit as regulated by state law.
Is jail credit applied to all arrests?
No, jail credit only applies to Class C misdemeanors.
Will city employees recommend a bail bond company or an attorney?
No, but most bail bond companies and attorneys can be located in the phone book, on the internet, or by using directory assistance.
Where are impounded vehicles stored?
The City of Keller, Southlake, and Colleyville wrecker information is listed below. Inquiries concerning fees, method of payment, and directions to the storage facility should be directed to the towing company. Be prepared to provide information such as the vehicle's year, make, model, and license plate number.
The towing companies used are:
Keller PD - AA Wrecker Service (817) 656-0104
Southlake PD - B&B Wrecker Service (817) 283-2121
Colleyville PD - B&B Wrecker Service (817) 283-2121 & Milners Impound Lot (817) 590-0308 or (817) 788-9770
If you still have questions about your vehicle, please contact the law enforcement agency that impounded the vehicle.
When may I visit a prisoner?
Visitation generally occurs from 8pm-9pm, 7 days a week, but is subject to cancellation, without notice, by the jail staff.
- Visitation requirements are:
- Visitation is limited to 15 minutes with a maximum of 2 people allowed per visit, per inmate.
- Visitors must be appropriately dressed, be at least 18 years of age, present a valid driver's license or state issued identification card and have no outstanding arrest warrants.
- Children under the age of 17 may not visit an inmate and children must not be left unattended in the jail lobby or in a vehicle while a parent visits an inmate.
- All visitors may be searched prior to entering the visitation room. Visitors are encouraged to call (817) 743-4770 to verify approval.
What items may I bring a prisoner?
Personal items of convenience and money may not be contributed to the prisoner's property. Prisoner prescription medication will be accepted and dispensed in the prescribed manner.
May a prisoner make free phone calls?
Prisoners are allowed to place one free 2 minute telephone call after the booking process has been completed. A prisoner also has the opportunity to make collect phone calls from inside cell blocks.
How to receive a collect call from an inmate?
To receive a collect call from an inmate, please call GTL Advance Pay at 1-800-483-8314 or visit their website at www.connectnetwork.com to set up your collect call account or to learn more. This will allow the inmate to call you on your landline or cell phone from GTL phones provided within our facility.
How may I get a prisoner's property?
Prisoner property can only be released after the prisoner grants written permission authorizing the release.
- To claim prisoner property, it is necessary to:
- Complete a property release form (which the jail stall will complete upon release)
- Present a driver's license or a state issued identification card.
What does it mean when a person is "booked" into jail?
This is an administrative process documenting a prisoner's entrance into the jail. The process includes collecting information such as person's name, address, reason for the arrest (criminal charge (s)), and inventorying personal property. A photograph and fingerprints are taken as well.
What occurs during an arraignment?
An arraignment is a formal meeting between a judge and a prisoner. The judge informs the prisoner of his or her charge(s), reads the prisoner his or her rights, and advises the prisoner of the bond amount. If the prisoner qualifies, an attorney is also appointed during arraignment.
When are prisoners arraigned?
Arraignments are generally held between 8am and 230pm; but can occur at alternate times to accommodate magistrate schedules.
What is an Alias warrant?
This is a warrant where no plea has been entered. A prisoner, bail bond company, or an attorney can post a bond for this type of warrant. The bond assures that the prisoner will appear in court.
What is a Capias warrant?
This is a warrant where a plea has been entered and the prisoner did not follow through with the court agreement. The only way to resolve a Capias warrant is to pay the fine in full or be released "time served" by remaining in jail until enough jail credit has been earned.
How long will a prisoner remain in the Keller Regional Detention Facility for a Class C misdemeanor warrants issued out of another city?
The city that issued the warrant has 24 hours, once notified, to pick up a prisoner. If the issuing city fails to meet this deadline, the prisoner will be released and the warrants will be reinstated or in some circumstances an extension of time may be granted.
Where can prisoners be picked up once released from the Keller Regional Detention Facility?
Prisoners are released in the jail lobby located at 330 Rufe Snow Drive, Keller, TX 76248.


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Keeping your property through a bankruptcy
It is a pretty common misconception that folks have that they can "bankrupt" only certain debts or include only certain items of property they have in a Chapter 7 or Chapter 13 bankruptcy. The truth is that every single debt, even the one to Aunt May for the bookshelf you bought from her, and all your property, even the rickety old bookshelf that you got from Aunt May, must be included. All that you owe and all that you own goes into an estate. The trustee is the party responsible for handling the estate in such a way that maximizes what the creditors in the case get. Exemptions are your way of holding onto property and are detailed in 11 U.S.C. Section 522.
Now, obviously people thinking of filing bankruptcy want to keep their stuff. I am often asked whether the trustee will come into their home and take things from them. Except for an incredibly unusual circumstance, the answer to that is "no". The bankruptcy code gives you exemptions which allow you to keep a lot of property. Some states, such as Kentucky, allow you to choose between state law exemptions or federal law exemptions. For most situations, the federal exemptions are more generous than the Kentucky exemptions. Under the federal exemptions, the vast majority of debtors are able to keep all of their property.
A recent Supreme Court of the United States case decided on June 17th, 2010, Schwab v. Reilly, 130 S.Ct. 2652, clarified exactly how the exemption works. While this clarification will not impact you, the average debtor, in any noticeable way, it is an important distinction for your attorney to know. When you claim an exemption you are actually exempting a dollar value of your interest in the property and not the property itself. The property remains in the bankruptcy estate until the trustee abandons the property or the case is closed.
The fact that you are exempting a dollar value interest in the property has some practical implications. For example, you should not dispose of (sell, destroy, give away) any property that you had when you filed the bankruptcy without checking with your attorney. Now, no one cares if you throw away your old skivvies, but you should check before getting rid of anything of substantial value. Another implication is that if you have a piece of property or asset that is likely to increase in value quickly, your attorney may need to move to have the trustee abandon the estates interest if it appears your case will be open for an extended time.
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A federal judge in a wrongful death lawsuit against the city of Burlington granted a request by an Iowa watchdog group to intervene in the case.
U.S. Senior Judge James Gritzner on Friday granted the request to intervene by the Iowa Freedom of Information Council, but has deferred the watchdog group's request to unseal police body camera footage and court documents and evidence introduced in the wrongful death of Autumn Steele, 34, of Burlington. The video and documents had been filed under seal in the wrongful death lawsuit.
Steele was mistakenly shot and killed in January 2015 by Burlington police officer Jesse Hill who said he perceived a threat from the family's dog while responding to a domestic disturbance.
The city of Burlington had opposed the request by the FOI Council to intervene, while Steele's family has not.
Gritzner will wait one week to see if any other groups now seek to intervene before additional proceedings, the ruling states.
Following Steele's death, her family and Burlington Hawk Eye filed separate complaints with the Iowa Public Information Board requesting police body camera videos, squad car dashboard camera videos, 911 calls and investigative files.
However, Burlington police and the Iowa Division of Criminal Investigation have denied the requests to release information, saying the materials were investigative files that did not have to be released to the public.
Despite the tentative settlement, the Steele family has continued to pursue, along with the Hawk Eye, the release of those documents through the IPIB.
A hearing on the IPIB complaints is scheduled for July 20 before Administrative Law Judge Karen Doland. She will turn over her findings to the nine-member IPIB for a final decision.


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Sarah McLachlan
CD | Christmas | 09 Oct 2015
Sarah McLachlan
CD | Christmas | 09 Oct 2015
Currently out of stock - Ships within 5-7 business days
Not available
This product is a member of the following groups/offers:
Nothing brings back cherished holiday memories—and creates new ones—like classic Christmas songs. The Classic Christmas Album series gathers a career's worth of those precious holiday memories from a legendary superstar on each of these special seasonal CDs.
Title: Classic Christmas Album
Format: CD
Release Date: 09 Oct 2015
Artist: Sarah McLachlan
Sku: 2302892
Catalogue No: 88843086482
Category: Christmas
Disc Count: 1
Transfer Format: Compact Disc
Video Format: Christmas, Holiday & Wedding
Primary Audio: 88843086482
Language: 0888430864825
Subtitles: Sony Music
Have Yourself A Merry Little Christmas
What Child Is This? (greensleeves)
I'll Be Home For Christmas
God Rest Ye Merry Gentlemen / We Three Kings - Barenaked Ladies With Sarah Mclachlan
Happy Xmas (war Is Over) - Sarah Mclachlan Feat. The Sarah Mclachlan Music Outreach Children's Choir And Youth Choir
Prayer Of St. Francis - Sarah Mclachlan With The Sarah Mclachlan School Choir
I Heard The Bells On Christmas Day
Space On The Couch For Two - Sarah Mclachlan With The Sarah Mclachlan School Choir
Find Your Voice - Sarah Mclachlan With The Sarah Mclachlan School Choir
Silent Night
O Little Town Of Bethlehem
The First Noel / Mary Mary
Song For A Winter's Night
In A Bleak Mid Winter
Christmas Time Is Here - Sarah Mclachlan Feat. Diana Krall


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Airport Employee Critically Injured by Moving Propeller
Impacting Travel United Airlines Monica Poling September 03, 2017
An employee at Newark Liberty International Airport has been critically injured after she was struck by a moving propeller early Saturday morning.
A spokesperson for the New Jersey Police Department, Joseph Pentangelo confirmed that the incident occurred at 2:20 a.m. Saturday morning in one of the airport's maintenance areas, according to NBC New York.
Although details are sparse, the worker—a 54-year old cleaner with a private company called Prime Flight—sustained a head injury during the incident and was rushed to an area hospital where she was listed in critical condition.
The aircraft involved is operated by CommutAir, an Ohio-based company that licenses flights for United Express, the regional carrier arm for United Airlines.
READ MORE: LaGuardia Project Brings in the Stars, Billions
"We are saddened to learn that a Prime Flight employee was seriously injured at Newark International Airport," said United in a statement. "Our thoughts and prayers are with her and her family and colleagues. We are working closely with Prime Flight, CommutAir and local authorities to determine how this occurred."
Because the incident occurred in a maintenance area, no flights were delayed. The accident remains under investigation.
For more information on United Airlines, New Jersey
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Listen, we don't know if ghosts exist. But if they do we know they want us talking about them on a Segway tour. Our guided Segway Ghost Tour features a bunch of stories we definitely didn't make up about the spirited side of the Distillery District. This tour is sometimes in dark conditions, please dress for the weather. If you survive, we even have a bone-shaking treat for you at the end!
The tour meeting point is in the Distillery District at our tour office. Start off with instruction and one-on-one training on how to use the Segway PT. The tour is usually conducted in the evening and is a great way to learn about the darker side of the site mixed with some history along the way. The Gooderham and Worts Distillery started in 1832 and many of the original buildings from the late 1800s are still standing. Many of the buildings have had strange occurrences over the years, with some employees vowing never to work alone or into the night. Stops include sites within the Distillery District where ghosts have appeared or mysterious events have occurred. The tour is entirely outdoors (dress for the weather) and suitable for ages 12 years and up. The experience includes training, plus about 40min of touring.


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I recently had a life changing event. I went to an Ayahuasca Healing Ceremony, I must say this wasn't the first time, but it was definitely the first time I experienced this… nothingness.
For those of you who don't know, when a person consumes this plant, this divine…mother of all plants. One will start seeing and feeling indescribable things. Visions, emotions and many more feelings will manifest depending on what you need to heal emotionally, spiritually and physically.
Every experience is different and no one can actually tell you what will happen during and under the effect of this beautiful healing medicine. But I must admit this, that night something happened in me, while I was under the influence, that made me feel scared, lost and almost regret going to the ceremony.
This is what happened…
I drank it. I waited to feel the impact, so far so good. Then I started seeing visions of multiple colors and patterns, it was beautiful. I felt my face smiling, at that moment I didn't really know what was happening or what I was feeling but I was happy, and wanted to keep this high forever. Then I envisioned myself not physically, but I could see my soul walking through all of these colors, stars and galaxies. As I moved along this path I saw that it was coming to an end, I was reaching the end of the stars and wasn't able to see what was next, since everything was completely dark and empty. Feeling adventurous I decided to keep going since I was feeling safe, secure in the arms of Mother Earth and hearing the shamanic chants and songs only reassured me that it was okay to go and explore the universe I was in.
I kept going, I remembered feeling scared and cold, I kept turning around to see if the previous path was still there, it was so, I kept walking. Suddenly I felt my legs painfully burning but I kept moving forward until I got to the point where I couldn't see anything or hear anything. I was lost in nothingness. I remember trying to move rapidly but there was no point, I couldn't see a thing. I felt scared; nothing had me prepared for this. I was in the nothingness, a black hole and I was scared I had gotten myself lost in the mere absence of existence and I wouldn't be able to come back. I'm not sure how long I was in there for but it felt like an eternity. I was crying, terrified, cold and for the first time ever I felt completely alone. I didn't know what to do, what could I do? No one was aware of what was happening and even if they knew what could they possibly do to bring me back? I thought to myself.
We always picture Hell as a burning place with demons of all kinds… Well this was definitely a type of Hell I wish no one ever has to encounter. After some endless eternities had passed I made a decision. A true empowering realization; I was light, and God was with me and I was stronger than any darkness because my soul was divine. As I write this I realize that I wasn't those entire things just back then and there, I AM those things. We all are, we just need to remember it and embody it.
So I envisioned myself as light and love and I pushed through the darkness… and I conquered it. I defeated my own darkness, I was back again in the ceremony with my loving self, and I realized; that was the journey, the place I needed to go, that was my healing task. I need to come into acceptance of this, fall completely into self-faith, so I could lose all fear. Fear of failing, of being alone, of being in the darkness of not knowing what to do, or what my next step should be. I needed to reassure my faith, faith in myself, faith in trusting that I am a pure being, full of light, love and gratefulness. Ready and hungry for life, trusting that nothing can ever and will ever be a mistake, there are no mistakes. Everything is always as it should be and I'm exactly where I need to be, everything is perfect.
We are blessed beings, living in light and love. We just need a little reminder every now and then.
Mexican, Spiritual Seeker, Light Worker, Reiki Healer, Blessed.
IG: catygtz


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Industrial hoses are small pipe or pipeline used for carrying or transferring various fluids (water, gas, chemicals) from a source to machines and other applications. Most of these machines are either hydraulic or pneumatic in nature. Industrial hose assemblies include a hose, fittings, and a way to fix the hose into the fittings. In most of the industrial hose assemblies, the fittings have barbed ends that are fixed into the hose. Multiple types of materials are available for both industrial hose and its associated fittings for different applications. Certain things to be considered while choosing the material for industrial hose assemblies are chemical compatibility, flexibility, erosion and cost.
Different Materials for Industrial Hose Assemblies
Rubber: It is the most common material for industrial hose assemblies and other mobile machines. Rubber hose serves as plumbing route for hydraulic fluid between various fluid-power components such as tanks, pumps, valves, cylinders, and others. Industrial hose made from rubber is easy to route and install which also absorbs vibration and lowers noises. Hose assemblies or fittings are generally attached to the ends and are simple to make. By the type of reinforcement, rubber hoses are often referred to as braided or spiral. Braided industrial hoses are constructed by cross-braiding alternating rows of wires at particular opposing angle around the tube. Textile wires are used in low pressure rubber hose while braided steel-wire hoses are applicable for industrial machinery, construction equipment, fleet vehicles, and heavy-duty trucks. Spiral rubber hose are used for high pressure applications and they have tightly looped, alternating spiral layers of steel wire.
Metal: In industrial hose assemblies made of metals, hose fittings are welded onto the industrial hose. They are capable of withstanding high pressure and greater range of temperature. Metal hose assemblies have longer product life and their applications include petroleum industry, chemical plants, steel plants, power generation industry, and also in heating, ventilation, and air conditioning systems.
Plastic: Polytetrafluoroethylene or PTFE is also widely use in the construction of industrial hose assemblies which include PTFE hose liner and stainless steel fittings. They are mostly used in sanitary manufacturing process and also ensure clean processing. As PTFE based industrial hose assemblies are designed specifically for easy cleaning and draining, they do not impart any odor to the media being processed. PTFE hose assemblies are also ideal for food, beverage, and pharmaceutical industries as they prevent entry or growth of bacteria that can lead to contamination.
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The Pocket Watch Database has compiled data covering the major American pocket watch manufacturers and created an easy way to find information using the serial number on the watch movement. Here are a few tips to find information about your pocket watch:
The following gallery contains Hamilton case models that would have been paired with railroad movements at the factory starting in the mid-1920s. Grades commonly found paired with these cases are the 992, 992E, 992B, 950, and 950B.


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WILMINGTON, NC (WECT) - It's Friday! What a week... So much like spring! Ready for the weekend? Let us help you get after it with your Cape Fear Region weather forecast. Two fronts play will play the lead roles. First, a passing cold front will engineer a dramatic drop in temperatures between Friday and Saturday. Then, an arcing warm front will generate the most substantial rain chances of the forecast period by early next week. As you eye your First Alert Planning Forecast panels, please consider these highlights:
- From short sleeves to heavy jackets... When you open your free WECT Weather App and tap your location specific hour by hour forecast, you will notice a steady, purposeful drop in temperatures between Friday and Saturday morning. 70s... 60s... 50s... 40s... ultimately 30s...
- Odds you will need an umbrella... Rain chances will be slim along the aforementioned cold front: at best 10 or 20% Friday afternoon and night. Notice more bullish shower chances in the parts of the forecast governed by said warm front: 50% Monday, for example.
- Can we look back on this remarkable week for a second? Winter definitely took a break! Each day, temperatures crested at least in the 60s and 70s and, amazingly, Wilmington Airport obliterated the existing record high on Thursday, February 7. Previous record: 75. Actual high: 83. Wow!!!
Copyright 2019 WECT. All rights reserved.


text: Steve's family has owned the same 1966 Caddy Convertible since he was five. He and his wife even took it to their prom! Now he commissions Danny and the team to restore it back to its glory days. But a tight budget turns this project into a real challenge. Meanwhile Ryan and Mike are hunting for a flip and spot a rare 1964 Buick Skylark.


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8. Lead Plant
This is the kind of the flower that spreads easily on the ground. It continues blooming even in late autumn adorning the garden with heavenly beauty. Besides, this flower can still bloom in shade, probably, not as fast as in the sun, but you'll still be able to appreciate its beauty.
9. Bellflower
As the name itself suggests, these flowers have the shape of the bell and look astounding. They come in all varieties and in different shades of blue. The flowers look as if they 'came' from some fairy-tale and create the mood of mystery and sheer astonishment.
They are perfect for home decoration or for making bouquets. Indeed, who wouldn't like to admire such beauty at home?
10. Salvia
These flowers usually attract hummingbirds, and what can be more mesmerizing that having these flowers and birds in your garden? Besides, they are heat and drought resistant, so you wouldn't have to worry about the changing weather condition and how the flowers might react to it.
And these flowers can bring life to the walls of the house, they will bring radiance and create positive mood all around.