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Electronic Royalty free music
Bold with heavy and gritty textures, this album of royalty free music shows fully synthetic sonorities, pulsing rhythms, and building grooves that create mystery, determination, and confidence.
Atmospheric, warm and bouncy textures meet energetic dance beats creating celebratory and cheerful moods that depict lounge scenes. EDM royalty free music .
Bright and upbeat royalty free music with highly rhythmic textures and building dance beats that create a hypnotic mood and depict dreamy landscapes.
Bouncy and driving with bright textures and animated dance grooves that create a mood of playfulness and depict exotic vacation scenes.
Upbeat and driving featuring synthetic sonorities, pumping grooves and atmospheric elements creating a futuristic and hypnotic feel that feed moods of determination, mystery and suspense.
Bright and upbeat with full synthetic sonority containing highly energetic Electronica and Rave elements that create a driving, bouncy and pulsing groove keeping a positive, enthusiastic and playful mood.
Bouncy and driving with some dark textures, this album of royalty free electronic music cantains full synthetic sonority, atmospheric synth pads and driving drum loops create a progressive feel and depict scenes of innovation and technology ...Learn more...
An upbeat and energetic collection of royalty free music with pulsing electronic textures, some Dubstep elements that emphasize a synthetic sonority creating moods of adventure, action, and urgency.
Pumping and highly energetic, featuring pulsing electronic textures and " four on the floor" drum loop grooves that create a driving and determined mood, depiciting futuristic scenes of innovation and technology.
Dreamy and spacious, featuring building ambient grooves, introspective melodies, light guitar textures and atmospheric synth pads that create a relaxed and hypnotic mood.
Mostly smooth and ethereal with dreamy atmospheres, hypnotic grooves and light drones, this album contains synthetic sonorities and an ambient feel.
Mostly spacious with hypnotic grooves, heavy synth and atmospheric textures creates moods of intrigue, discovery, and determination.
Highly atmospheric with fully synthetic sonorities, driving and building drum loop grooves intertwined with spacious and pulsing synth textures create a determined mood.
Ethereal and spacious introductions lead to ambient and hypnotic grooves, featuring dreamy and atmospheric textures with light ethnic influences that create an introspective mood.
Laidback and mellow, featuring and underlying funk grooves, warm piano textures and Jazz melodies that create a vintage feel.
Pulsing and atmospheric, featuring light drones and synth pads with a fully synthetic sonority that creates an introspective mood, depicting futuristic scenes.
Ambient and warm, featuring light drum loop grooves, rhythmic percussion and smooth keyboard textures create a laidback and mellow mood.
Mostly downtempo and laidback with Chillout influences, featuring full synthetic sonorities, warm textures and light percussion rhythms that create an ambient mood.


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German retail sales drop 1.6% in January
German retail sales plunged 1.6% in the month of January compared with median forecasts of 0.5% advance. On the other hand, December's reading of -1.4% was upwardly revised to 0.1%. The year on year reading, however, showed 1.6% rise from a revised of 0.3%, which was -0.9% initially, while analysts' estimates referred to 0.2%.

subset: commoncrawl


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Oasis Maspalomas Apartment
Avenida Del Oasis, 14, Maspalomas (Gran Canaria), Spain
Surrounded by the lighthouse and the tower, Oasis Maspalomas Hotel offers 100 guestrooms featuring a fantastic view. This quiet hotel dates from 1975 and was refurbished in 2006.
The city center is 30 minutes' walk of the property. The spectacular beach stands 100 meters away.
Palmitos Park is 2.5 km from the hotel and Botanical Park of Maspalomas (El Parque Botanico de Maspalomas) is 2.6 km away.
All comfortable guestrooms come with cable TV, a microwave, a refrigerator, a safe and a private balcony along with fresh décor. Many guestrooms offer views of the Atlantic Ocean. All bathrooms are appointed with a shower and a hairdryer.
Guests can reach Oasis Maspalomas Hotel by car from Gran Canaria airport in 30 minutes.
Bicycles and cars are available for rent at Oasis Maspalomas Hotel.
A solarium and a Jacuzzi are offered for relaxation. Such activities as surfing and tennis are also provided.
Important information
- Children and extra beds
- All children under the age of 2 may stay for free in a crib.
- There are no extra beds provided in a room.
- Indoor Parking
- Wi-Fi/Wireless LAN
- 24-Hour Front Desk
- Safety Deposit Box
- Terrace
- Garden
- Elevator
- Credit Card Accepted
- No Pets Allowed
- Bar
Leisure & Sports
- Outdoor Swimming Pool
- Casino
- Jacuzzi
- Golf course
- Paid Shuttle Service
- Concierge
- Tours/ticket assistance
- Currency Exchange
- Medical Service
- Shops/Commercial Services
- Car Rental
- Bicycle Rental
Room Amenities
- Air Conditioning
- Cable/satellite television
- Movies (Pay Per View)
- Separate dining area
- Kitchen
- Balcony
- Sofa Bed
- Refrigerator
- Microwave
- Toaster
- Tea & Coffee Facilities
- Cookware & kitchen utensils
- Direct dial telephone
- Private Bathroom
- Bathtub
- Hair dryer
Avenida Del Oasis, 14, Maspalomas (Gran Canaria), Spain
All comfortable guestrooms come with cable TV, a microwave, a refrigerator, a safe and a private balcony along with fresh décor. Many guestrooms offer views of the Atlantic Ocean. All bathrooms are appointed with a shower and a hairdryer.
Please, select dates to see available rooms.
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Oasis Maspalomas
Avenida Del Oasis, 14, Maspalomas (Gran Canaria), Spain
What's nearby
- Local attractions
- La Charca300 m
- El Faro de Maspalomas500 m
- Playa de Maspalomas550 m
- Centro Comercial Faro 22.1 km
- Biceps Gym Fitness Studio2.2 km
- Campo del Golf de Maspalomas2.3 km
- Palmitos Park2.5 km
- Iglesia Evangelica Alemana2.7 km
- Parroquia de la Santisima Trinidad3.6 km
- Asociacion Cultural Cocineros Reposteros y Panaderos3.6 km
- Discos Noda3.4 km
- Carbi93 S.A.2.3 km
- Dunas Maspalomas600 m
- Airports
- Gran Canaria29.8 km


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First Cruise
Sail Date: March 21, 2011
Reviewed: 6 years ago
Traveled As: Couple
Room Type: Oceanview
Pros: Quality of service
Cons: Quality of the Handicap ajustments
Recommends Monarch of the Seas (RETIRED) 221? Yes!
Category Ratings
Click table then swipe to view all columns.
Click table then swipe to view all columns.
|Ship Quality
|Handicap accomodations could be improved.
|Food and Dining
|Service Staff provided excellent service
|Cabin / Stateroom
|Not totally handicap updated.
|Service and Staff


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Axago USB 3.0 - SATA 3.5 "External ALINE box
Compact aluminum external box Axago EE35-XA3 ALINEbox with high-speed USB 3.0 interface designed for 3.5 "SATA drives.
Durable cast aluminum housing for perfect mechanical drive protection and excellent heat dissipation.
Discretely molded housing with a brushed finish provides an elegant style.
Simple mounting the hard disk and automatically install and manage a complete layman.
- Compatible with USB 3.0, backward compatible with USB 2.0.
- Support transfer rate of 12/480/5000 Mbit/s (full/high/super speed).
- Support 3.5 "SATA III/II/I drives of all capacities.
- Compliant with Serial ATA 2.0 specification (SATA/300), transfer rate up to 3 Gb/s (300 MB/s).
- Compliant with Serial ATA 1.0 specification (SATA/150), transfer rate up to 1.5 Gb/s (150 MB/s).
- Total bandwidth/bit rate is determined by the used box SATA/300 interface (teor. 3 Gbit/s).
- Connection Options for running with the support of Hot Plug.
- Full support plug and play.
- Connection via a 'major' USB 3.0 type B female connector.
- USB 3.0 cable USB type A male/type B male USB length of 80 cm included.
- Subtle blue LED indicating connection to the USB port of a computer (permanently lit) and data transmission (flashing).
- Power over compact external AC adapter 12V - 2A, with integrated prong plug a cable length of 150 cm.
- The power switch on the rear panel of the box.
- Durable box body is cast from aluminum alloy head box made of plastic.
- Simple installation disk - the disk is mounted guide rails disk is connected directly to the motherboard SATA connector retracts into the body and face of the box is fixed with two screws with the supplied screwdriver.
For more information:
- The compact dimensions of the box 180 x 120 x 32 mm, AC adapter Dimensions 74 x 36 x 91 mm (incl. plug).
- Weight external box 244 g (without cable and disc), AC adapter weight 128 g (including cable).
Supported operating systems:
- MS Windows XP/Vista/7/8/8.1 and later, Windows Server 2003/2008/2012 and newer (all supported 32 - and 64-bit versions of Windows), Mac OS X 10.x or later, Linux Kernel 6.2 xa higher.
- Note: Drivers are part of the supported operating systems and will be installed automatically. No other driver installation is needed and are not available.
Package includes:
- external box
- USB 3.0 cable length of 80 cm
- mounting rails, screws and screwdriver
- Stand upright box to allow traffic
- 12V/2A power adapter
- multilingual manual
- packed in a cardboard box with a clearly specified parameters
Additional Information
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Untamola Blues(2008)
A novel by Joseph Damrell
Untamola is the dreamworld from the Finnish epic, Kalevala, which Damrell evokes in the fictional Lake Superior port town in Michigan's Upper Peninsula. United by circumstance, random happenstance, gullibility, and plain bad luck, the mostly Finnish American and Native American characters seek a dubious fortune in a desperate, illegal venture on the high seas. But since fortune tends to favor the foolish although seldom as they anticipated it all get their reward. In its use of classic comedy as a subversive, moral force exposing the limitations and strictures of self-image and societal norms this tale is a wild, hilarious, and at the same time, poignant take on the human condition.
Used availability for Joseph Damrell's Untamola Blues
See all available used copies of this book at Abebooks UK or Abebooks US
Paperback Editions
June 2008 : USA Perfect Paperback
|Title: Untamola Blues
Author(s): Joseph Damrell
ISBN: 0-87839-280-7 / 978-0-87839-280-3 (USA edition)
Publisher: North Star Press of St. Cloud, Inc.
Availability: Amazon Amazon UK Amazon CA
Genre Pages


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Did You Know?
- Nearly 200 high schools in Wisconsin have students participating on a high school team.
- Nationally, over 2,000 high school students bowl on a team each year.
- 250+ colleges offer bowling & nearly half offer scholarships.
- Nationally, 54,000 kids bowl in high school varsity competition.
- Bowling is the fastest-growing high school sport this decade and continues to gain more participation every year that it is offered.
- The bowling industry provides more than $6 million in scholarship each year.
Bowling also gives students an opportunity to become involved with their high schools, teaches fundamentals for a lifetime sport and helps athletes earn scholarships and bowl in college.
Find out why bowling is a sport that lasts a lifetime.
2017 Finalists
State Tournament
March 2 – 4, 2018
Dale's Weston Lanes
5902 Schofield Ave., Weston, WI
2017 Results
FINAL SCORES-Boys Singles
FINAL SCORES-Girls Singles
FINAL SCORES-Boys Team Division l
FINAL SCORES-Boys Team Division ll
FINAL SCORES-Girls Team Division I
FINAL SCORES-Girls Team Division ll
State Tournament Local Information
Bowl Stream TV
High School & State Tournament Forms/Rules
Policies and Procedures July 2016
Proposal for Policy and Procedures Rule Changes
Team Information Form
High School Proof Form
High School Bowling Club Co-op Application
Athletic Code of Conduct 2016
Travel Authorization Form
High School Center Transfer Form 2016
High School Bowler Transfer Form 2016
High School Scoring Form
High School Picture Release Form
High School Picture Order Form
2016-17 Boys & Co-ed Varsity Teams Population Totals
2016-17 Girls Varsity Teams Population Totals
District State Singles Roster
State Team Roster
Coaches Information
Storm Scholarship Doubles
March 4, 2018
Dale's Weston Lanes
5902 Schofield Ave., Weston, WI
2017 GIRLS Storm Scholarship Doubles Results
2017 BOYS-COED Storm Scholarship Doubles Results REV 3/15/17
2017 Storm Scholarship Doubles Entry Form/Rules
Storm Scholarship Request for Funds Form (2015 & Earlier)
Wisconsin All Star Team Challenge
March TBD, 2018


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'The Voice' goes live for first time this season
On NBC's The Voice, after weeks of blind auditions and vocal battles, 12 of the 24 finalists took the stage Monday for the first live show of the season. Celebrity coaches Blake Shelton and Christina Aguilera watched the six members of their teams sing for America's votes.
On Tuesday (9 ET/PT), the lowest three vote-getters from each team sing for their lives, and the coaches decide which two from each team head home.
Next Monday, Team Cee Lo Green and Team Adam Levine face the music.
How Monday sounded:
Team Blake
Former Alicia Keys backup singer Jermaine Paul, who emerged onstage in a cloud of smoke, offered a dramatic rendition of Bon Jovi's Livin' on a Prayer. Christina told Jermaine, "You worked the stage," but she felt little connection with the song. Coach Blake disagreed: "You're making a connection. People love you … (especially) girls."
Seventeen-year-old RaeLynn put a country twist on Wake Up Call by Adam Levine's Maroon 5. Adam said the showing was "so darn good," and Christina said she was impressed with the young artist's stage presence. Blake told RaeLynn, "You're representing a new generation of country music so well."
Reggae street performer Naia Kete took a tender approach to Adele's Turning Tables. Christina said she would have liked more reggae, but Blake said Naia proved she was more than just reggae, and had special vulnerable moments.
Rock 'n' roll crooner Jordis Unga belted Alone by Heart. Cee Lo thought the overall staging and performance were good, and Blake was happy the "amazing" Jordis didn't play it safe.
An emotional Erin Willett, whose father recently passed away, sang a soulful Living for the City by Stevie Wonder. Cee Lo called Erin a "champion" with "so much soul and charisma." Blake said Erin had the best technical and most passionate performance of the night.
Charlotte Sometimes showed off her range with Paramore's Misery Business. Adam praised Charlotte for her unique sound but said he heard "some flatness." Christina said the performance had the right balance of drama and subtlety. Blake said he "didn't hear any pitch problems" and called Charlotte a "true artist."
Team Christina
Opera singer Chris Mann sang a chill-inducing Bridge Over Troubled Water. Christina praised the singer for his uniqueness and vocal trills. Cee Lo said, "I wanted to be critical … but I really, really enjoyed that." Adam, though skeptical about the song choice, was "really happy" with the performance.
Moses Stone rapped Kanye West's Stronger and Power with plenty of chutzpah. "You're a natural on stage," said Cee Lo. "You know how to entertain, but I want to see you singing more," said Adam. Blake noted how Moses' vocals had improved and Christina said she loves the way he raps, no autotune necessary.
Indie-rocking Lindsey Paveo's breathy Somebody That I Used to Know by Gotye had an "amazing vibe" but was missing some power, said Adam. Christina praised the singer's arranging skills and said, "I could listen to you all day."
Sera Hill, surrounded by bare-chested men, sounded like Mary J. Blige when she sang Find Your Love by Drake. Blake was distracted by the "male strippers" but said Sera did great. Christina told Sera she had great stage presence and "stepped it up."
Seventeen-year-old Ashley De La Rosa looked well beyond her years singing Right Through You by Alanis Morissette. Adam said Ashley was most improved and "did everything perfectly." Christina said, "You were phenomenal." A shocked Adam said to Christina, "We just got along. It's a miracle!"
The formerly homeless Jesse Campbell sang a smooth What a Wonderful World by Louis Armstrong. Cee Lo said, "If someone on my team doesn't win, I'm looking for you to win." Adam agreed: "You're the dude to beat," he said. Christina told Jesse he was fantastic.
Posted | Updated


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There is an urgent necessity for multinational corporations to articulate and comply with high ethical standards. Codes for corporate conduct can do much to advance this goal, and the "Guidelines for Multinational Enterprises" is a significant step in this process.
- © 1977 The Regents of the University of California
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Watch Coldplay's Chris Martin Cover Bruce Springsteen's 'Streets of Philadelphia'
Chris Martin played a solo acoustic version of Bruce Springsteen's hit during his band's stop in Philadelphia.
Chris Martin played a solo acoustic version of Bruce Springsteen's hit during his band's stop in Philadelphia.
During a concert in Seattle, Bruce Springsteen and the E Street Band brought out one of the locals to help perform the 'Born in the USA' classic.
Former home of Rock N Rock Hall of Fame member is up for sale. Want to buy it?
I have a sneaking suspicion that 50 Shades of Grey is going to be the #1 movie at the box office this weekend.
So you heard earlier this week that the man, the myth, the legendary parody maker 'Weird Al' Yankovic has returned to the airwaves! I know you're as excited as I am so I couldn't wait to share another release off his new album as well as a great interview with Yankovic which talks about making his parodies and specifically why he's never parodied "The Boss" Bruce Springsteen...
Apparently the Boss has been performing the songs of native artists at various international shows on this tour (i.e. AC/DC and INXS in Australia) and apparently he's a fan of Lorde's song "Royals...


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Office Space to Rent in Leighton Place, NW5
We have a variety of serviced offices available to rent in Leighton Place, NW5. Each office space is offered at the best rates and suited to your individual needs and requirements.
We have a variety of serviced offices available to rent in Leighton Place, NW5. Each office space is offered at the best rates and suited to your individual needs and requirements.
This contemporary warehouse conversion features large windows, natural wooden floors, minimal style, low voltage oversize spotlighting, aluminium fittings, 2 person office and open plan area for 4/6 workstations, fully Cat 5 servicing for broadband and communications, own kitchenette behind sliding wall, own toilet, own entrance.
A fascinating 19th century industrial building (originally a horse-drawn bus factory) close to Kings Cross and Camden with easy access to the city, offering a superb range of quirky office and studio space where the design and layout allow you to create your perfect workspace within. There are excellent opportunities for networking with other businesses, a regular calendar of events, not to mention their own restaurant, bar and theatre! A comprehensive number of services and facilities are provided with communal and co-working spaces, plus several spacious meeting rooms. Part of the building is also used as an art gallery for local artists to exhibit their work.
Serviced office accommodation near Kings Cross St Pancras Station offering clear, open floor plates, with good working height, appropriate to modern occupiers' requirements. Extensive refurbishment of the exterior parts has brought an ordered well run site, with security including 24 hour cctv. The estate is made up of 26 buildings, providing the opportunity for self contained and multiple occupation.
A rendered exterior with a gated archway to the central courtyard around which the business space is arranged, comprising of a variety of open-plan units; small offices and studios, some with wooden floors and exposed beams. The majority of the spaces are self-contained and have their own entrances making it a great office space for individual businesses.
This centre has the latest in telephone and data technology, and clients benefit from services and facilities including: Cat 5 cabling, high-speed internet, call diversion to mobile or PSTN, voice mail and call answering to name but a few. Included in the monthly service fee are: rent, rates, furniture, heating / air conditioning, electricity, cleaning and insurance. Additional features encompass meeting rooms, colour copiers, call handling and network printers.
The offices are based within a beautiful red-brick building with large windows. It boasts two meeting rooms, a printer room and a break out area for both high-level and informal meetings. Facilities include high-speed fibre optic internet, 5e structured cabling, 24/7 access into the property, reception & virtual office services and on-site parking.
SHARED SPACE ONLY We are offering space within a very large penthouse open plan office in Camden 3 mins from the tube. Space is a very large Penthouse office shared with partitions if required. Available immediately on flexible terms. We currently have 2 suitable banks of 8 (showing as banks of 6 in the pictures numbered) Included in rates: Rent Rates Use of 5 meeting rooms to modern trendy design Utilities Manned reception Office Cleaning & Maintenance Waste Disposal Furniture & Fixtures Leasing Internet Connection Phone Handsets and Line Rental Tea, Coffee, Sugar and Milk Office Manager to deal with queries Not included: Telephone Calls – itemised bills Printing and Copying – cost per print/copy (we will supply paper) Stationery Computer / IT services Cost: £350 per desk
On street permit parking @ £250+ pa...The centre provides accommodation and services to both companies and individuals, either on a short or long-term basis. The centre offers you a place to set up alone or start a business without having to worry about maintenance, repairs, cleaning, equipment and payment of rates or who answers the phone! The premises are in two buildings, front and rear, which overlook an attractive garden courtyard. There is both office / studio and storage space divided by partitions and screens into units from 100 sqft. There is 24-hour access by arrangement, and telephone, fax and photocopying is metered and charged accordingly. Amenities included in the monthly billing include: cleaning, telephone / reception, maintenance, water and general rates, furniture / carpets, fax / photocopying, refreshments, gardens and communal space. Secretarial, fax and courier services are also available on the premises.
This business centre is an immaculately refurbished iconic listed building that offers high quality managed workspace to suit all types of business. This centre can offer you a range of units to accommodate between 4 and 12+ people, with the flexibility to easily alter your workspace as your needs change. Additional support features include an impressive reception area, meeting room, communal breakout space, and an onsite centre manager to ensure the smooth running of your office space, enabling you to concentrate on your business. Minimum contracts start at just three months.
This centre is situated between a row of shops and offices on Crowndale Road. It is an airy and fresh working environment with convenient access to tubes and buses.
Below you can see the nearest areas to Leighton Place, NW5 where you can find serviced offices:


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Rhythm Divine - Enrique Iglesias Lyrics & Download MP3
Enrique Iglesias Rhythm Divine http://www.mediafire.com/?ugmy2jomynx download this or any youtube video as mp3/mp4 FAST and free at mp3ify.com.
Enrique Iglesias-Rhythm Divine Lyrics MP3
Enrique Iglesias Rhythm divine For more Lyrics Subscribe me and write in the comments box lyrics that you want and I will make them ;) Enjoy.
rhythm divine song with lyrics MP3
hi, this is rhythm divine with lyrics .This is purely a fan made video .I dont own this song it belongs to its respective owners. Enjoy.
Rhythm Divine MP3
Music Video By Enrique Iglesias (C) 2005 Interscope Records Pop. Get Enriques new Spanish single Cuando Me Enamoro on iTunes: http://bit.ly/aB4Qhq.
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Download "Rhythm Divine" in the style of Enrique Iglesias in MP4 or MP3+G formats available here: https://karaoke.stingray.com/search/song?
Enrique Iglesias - Rhythm Divine lyrics (HQ) MP3
This is one of the best songs that exist. Moreover, the lyrics is very romantic! Suscribe!!!!!
Enrique Iglesias Rhythm Divine Lyric Video MP3
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Enrique Iglesias - Rhythm Divine + lyrics MP3
Enrique Iglesias - Rythym Divine with lyrics at the side. Rhythm Divine Escucha el ritmo de tu corazon (Whispered) From the coast of Ipanema to the Island of ...
Enrique Iglesias Rhythm Divine lyrics MP3
Its a song by Enrique Iglesias named "rhythm divine" from his debut album "Enrique" Enjoy Y'all and don't forget to subscribe! I do not own the music!!! :)
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Watch The Whole Concert : http://www.youtube.com/view_play_list?p=F97B69E68A74E5CE [2007] Enrique Iglesias - Live From Odyssey Arena Belfast (DVD) ...
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Rhythm Divine karaoke video originally performed by Enrique Iglesias. Music Licensing, Backing Tracks, Karaoke available at http://www.singsongsmusic.com .
Enrique Iglesias | Rhythm Divine with lyrics MP3
Download this karaoke song (MP3, MP4, WMV or MP3+G formats available) http://www.thekaraokechannel.com/store/store.html?vid=20081002001&cid=404.
Yello & Shirley Bassey ~ The Rhythm Divine MP3
Yello & Shirley Bassey ~ The Rhythm Divine Lyrics: You know that my tears have kept me awake The longer you're gone I'll hunger and shake. From Warsaw to ...
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Enrique Iglesias ENRIQUE IGLESIAS enrique iglesias enrique IGLESIAS ENRIQUE iglesias.


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This 2-pack filters gives you the availability of a spare filter on hand whenever you need it. These filters offer great-tasting, chilled water from your refrigerator while filtering out chlorine taste and odor, cysts, lead, mercury and asbestos with the PuR® water filtration system. Doesn't use valuable space inside the refrigerator, and also conserves energy because the door doesn't have to be opened. Base-grille installation consists of a simple quarter turn to remove a used filter.
Protection Agreements
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7/2 View Avenue Langford 6147 WA
2 Bedroom Unit
Beautiful 2 bedrooms unit, neutral modern decor throughout.
Property consists of
*Tiles and carpet
*Open plan kitchen/meals and living area.
*Close to shops and transport.
Pets under 10kg considered
For All Enquiries Please Call LJ HOOKER CANNING VALE - 9398 4000
**Applications can be submitted via 1From. You must view the property to obtain verification code. Any questions please do not hesitate to call LJ Hooker Canning Vale 9398 4000**
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LJ Hooker | Canning Vale Thornlie
Thornlie WA, 6108
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Real Estate For Rent in Langford, WA 6147 priced between $200 and $360


text:
Islamabad, Dec.30 (ANI): Worried by the rising influnece of the Taliban in the tribal areas, Pakistan Prime Minister Yousuf Raza Gilani has said that the government would not support 'establishment of a parallel authority' in the country.
"Nobody would be allowed to challenge the writ of the government or to encourage militancy, so that NATO forces do not get an opportunity to sneak into Pakistan's territory," the Daily Times quoted Gilani, as saying.
Gilani also denied reports about government's secret agreement with the Taliban which states the Taliban will not create unrest in the tribal areas if troops are withdrawn from the Talibani stronghold tribal areas.He also claimed that Pakistan has offered all help and co-operation to India in the investigation of Mumbai terror attack.
Gilani refused to accept that the United Nations banned the Jamaat -ud- Dawa (Jud) due to continuous pressure from India after the Mumbai carnage.
"The matter was pending with the UN Security Council before the Mumbai attacks," he said. (ANI)


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News About Listed JC Roberts Stakes
Saturday March 5th Ascot Race Day Betting Update
4 March 2011
Ascot Racecourse is not to be forgotten this Saturday March 5th with a full day of racing planned in the far west including three Listed events.
We've had a look through Luxbet and found some great information to put together an Ascot Race Day Betting Update on the feature races.
The Listed Perth Stakes over 1100m is the first of ...


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Indore, Nov 16 (UNI) In order to ward off evil from one-day International cricket match between India and England scheduled here tomorrow in Usha Raje Stadium, the stadium authorities have been allegedly using charms.
A black earthen pot was put on a stick with a caution ''Buri nazar wale tere munh kala'' to save the ready pitch and lush green ground. However, when this superstitious act caught meadia attention then it was removed immediately.
Though the stadium authorities denied any comment.
In a match played between India and Sri Lanka the pitch was deteriorated merely after three overs 10 years ago and due to which match was forced to be closed.
Sources said that to avoid any such malfunctioning the superstition was practised in the stadium.


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Join us for Toddler Time at SkyZone Trampoline Park, Age 6 and Under from 10am to noon.
Toddler Time
If they can walk, they can jump! Toddler Time at Sky Zone is a special time just for the little ones, where they can explore exciting new ways to be active, all while staying safe. All Toddler Time activities are specifically designed for children.
Must be 6 years & younger
All toddlers must wear SkySocks$10/toddler, 1 parent participates for free with the purchase of SkySocks $2 (if court space allows)Monday, Wednesdays & Fridays -10am-12pm – Check calendar sections for times and cancellations
A Sky Zone liability waiver must be signed by each parent or legal guardianJumpers are still separated by size during Toddler Time. Parents can monitor their children from the viewing area or pads on the court, but cannot jump in the toddler area.


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Buy Jillian Michaels - Calgary Tickets
Q:When does jillian michaels comes to calgary and what will be the prices of the tickets?
A:Log on to our website to get all the information about Jillian Michaels Calgary Tickets and the event itself.
Policies | Contact Us | Terms | Blog
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D-Link's first HomeKit enabled camera allows users to see everything through their iOS devices. The product also has advanced security features with end-to-end encryption and authentication between the camera and an iPhone, iPad, or iPod touch.
- 180-Degree Field of View
- Dynamic Video Streaming–The video bitrate continuously adjusts to ensure optimal performance given the available bandwidth
- Two-Way Audio–Listen and speak using the camera's built-in microphone and speaker, so even when users are not at home they can still make their presence known
- Night Vision–See up to 16 feet in complete darkness with infrared night vision
- Local Recording–Record motion detection triggered video clips directly to a microSDXC card up to 128 GB. Offers more flexibility on recording video locally to the camera without impacting network or Internet bandwidth, all with no recurring monthly fees
- Omna App–Advanced features are available on the free Omna companion app
- Stylish Design–The camera is designed to fit into modern homes with elegant, discreet and stylish designs
- Motion Detection–Trigger alerts and automatic video clip recording


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30 April 2008,
London, King Street
Price realised GBP 3,500
Estimate GBP 1,200 - GBP 1,800
VERGILIUS MARO, Publius (70-19 BC). Bucolica, Georgica et Aeneis. Birmingham: John Baskerville, 1757.
4° (290 x 224mm). Contemporary red morocco gilt, covers with central lozenge made up of circles, stars, florets within wheels, and flower heads, double roll-tool dentelle borders, spine gilt in compartments with raised bands and green morocco lettering-piece, comb-marbled endpapers, gilt edges (joints a little rubbed, lettering-piece slightly chipped, small scuff marks to covers, some minor discolouration).
FIRST EDITION OF BASKERVILLE'S 'FIRST AND PERHAPS FINEST BOOK' (Gaskell), here in a fine English morocco binding. Work on the book was already in hand in 1754. Both Brunet and Renouard regarded it as the best and most sought after of the Baskerville quarto classics, marred only by the appearance of an inferior reimpression on cheaper paper, printed two or three years later but with unaltered date. Brunet V 1292: 'chef d'oeuvre de Baskerville'; Renouard, Bibliothèque d'un Amateur II 244; Gaskell I.
New York +1 212 636 2000
London +44 (0)20 7839 9060
Hong Kong +852 2760 1766
Shanghai +86 21 6355 1766
Special Notice
No VAT on hammer price or buyer's premium.


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That's a good one!
This is a discussion on New Bartender within the Off Topic & Humor Discussion forums, part of the The Back Porch category; A man walked into a very high-tech bar. As he sat down on a stool he noticed that the bartender was a robot. The robot ...
A man walked into a very high-tech bar. As he sat down on a stool he noticed that the bartender was a robot. The robot clicked to attention and asked, "Sir, what will you have?"
The man thought a moment then replied? "A martini, please". The robot clicked a couple of times and mixed the best martini the man had ever had. The robot then asked, "Sir, what is your IQ?"
The man answered "Oh, about 164." The robot then proceeded to discuss the 'theory of relativity', 'inter-stellar space travel', the latest medical breakthroughs', and other matters of equal import.
The man was most impressed. He left the bar but thought he would try a different tack. He returned and took a seat. Again the robot clicked to attention and asked what he would have. "A Martini please?" Again it was superb The robot again asked, "What is your IQ sir?"
This time the man answered, "Oh, about 100". So the robot started discussing NASCAR racing, the latest basketball scores, and what to expect the Dodgers to do this weekend.
The guy had to try it one more time. So he left, returned and took a stool.... Again a martini, and the question, "What is your IQ?"
This time the man drawled out " Uh..... bout 50"..??
The robot clicked then leaned close and very slowly asked , A-r-e y-o-u-r p-e-o-p-l-e g-o-i-n-g t-o n-o-m-i-n-a-t-e H-i-l-l-a-r-y-?
ΜΟΛΩΝ ΛΑΒΕ: Buy These Stickers Here
"A man without ethics is a wild beast loosed upon the world" Albert Camus
I love it
Don't hit at all if it is honorably possible to avoid hitting; but never hit soft!
-- Theodore Roosevelt --
There are over 550 million firearms in worldwide circulation. That's one firearm for every twelve people on the planet. The only question is: How do we arm the other 11? (Yuri Orlov [Nicolas Cage] Lord of War)
Now that is the funniest thing I have heard in a while!! LMAO
"Do not fear those who disagree with you; fear those that do and are too cowardly to admit it" - Napoleon
eschew obfuscation
The only thing that stops bad guys with guns is good guys with guns. SgtD
ΜΟΛΩΝ ΛΑΒΕ: Buy These Stickers Here
"A man without ethics is a wild beast loosed upon the world" Albert Camus


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Dog Walkers in Cedar Hill, NM
Book a dog walker to give your dog a 30-minute walk around your neighborhood.
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Dog Boarding, Drop-In Visits, Doggy Day Care, Dog Walking
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We know you can't always be there, and it's our goal is to connect you with sitters in your neighborhood who can help take care of your pet. With Rover, it's easy to find and book Cedar Hill dog walkers who will give your dog the exercise they need. Book a 30-minute walk one day, once a week, or even every day. That way, you can take care of what you need to do and know your dog is getting the love they deserve. No matter how often you need a sitter or dog walker, we're here for you. We're proud to offer complimentary premium insurance with dog walking services booked through Rover. The next time you're looking for a dog walker in Cedar Hill, NM, visit Rover to search for the perfect dog walker, set up a Meet & Greet, and book a walk anytime you need it. All you have to do after that is sit back and relax: your dog's in good hands.
Wondering what you should be paying for a walk? Check out our standard dog walking rates for a comparison.


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A security camera has captured a rather unexpected sight - a FedEx delivery man trying, and failing, to jump into his van as it rolls down a road.
It isn't quite clear how he ended up in the predicament as the video starts with the van driving up the hill and disappearing out of view.
There's a few moments pause during which some local dogs start to get very excited at something happening in the direction the van went.
Then you see it - first the van rolling down the hill, then the delivery man attempting to jump into the driver seat.
As the van gathers speed, seemingly hurtling towards a house, he soon gives up. Luckily the van narrowly misses the house - and a tree - instead crashing into a garden wall.
The identity of the driver, and the person who the security camera belongs to is currently unknown. According to the timestamp of the video the incident happened just before 10am on Thursday 3 April, in what looks to be a nondescript suburban area of the US. Reuse content


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Home > About us > How Cefic is organised > Jean Pelin
UIC is a fully active social partner, negotiating with Trade Unions and engaged in developing the right skills for the employers of its member companies.
UIC is a very dynamic member of both MEDEF at National level and CEFIC on the European scene.
Mr. PELIN is a very active member within the Cefic committees, being involved in several working groups and European Advocacy.
Before joining UIC in 1997, he held several executive functions, in the following companies: Cogema (1992-1997), SEDOC (1989-1992), ECONOCOM (1986-1989), Grundig (1980-1986)
Getting responsible chemicals management recognised throughout the entire supply chain of the chemical industry under a "proportionate framework"
» Learn more about Regulatory Framework
The 2016 Cefic European Facts & Figures provides an analysis of the latest competitive trends in the European chemical industry.
» Learn more about Facts and Figures
The Responsible Care ethic helps chemical companies to operate safely, profitably and with due care.
» Learn more about Responsible Care
Copyright 2017


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Introducing the perfect accessory for Apple's brilliant new iMac G5 and Cinema Display...
The iLift suspends the monitor weightlessly above the desktop, where it
can be easily moved with one hand. Save space and work ergonomically by
placing your monitor exactly where you need it.
*** Compatibility - Please refer to our Apple Compatibility Chart to ensure that you purchase the correct product for your Apple system.


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Graceful and simple, this stoneware serving bowl will coordinate beautifully with table and kitchen Decor.
Our team has over 100 years combined experience with contemporary lighting.
Every EuroStyleLighting.com order is backed by our 100% Satisfaction Pledge.
Shipping and Delivery Information - For item EU6H771 if ordered by 1 pm Pacific Time


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The nation has changed almost unbelievably since the 9/11 attacks that happened 13 years ago today. As a result, it seems the government has taken unprecedented steps in a blatant abuse of power all the while hiding behind the message of "securtiy" to act in a manner undoubtedly unconstitutional.
Just as so many Americans are becoming upset with the overreach of government in recent years, Judge Andrew Napolitano recently spoke out against Obama.
(See also: James Foley's Mother Slams Obama For Failing To Save Her Son)
The words came out during an interview with TPNN's Tim Constantine where they began by speaking about the, "erosion of our civil liberties." Bringing up the arguable notion as presented by the Attorney General's office wherein the president has the right to use drones in order to kill Americans on American soil, Napolitano ripped "murderous" Obama a new one:
"There is no more direct, and there is no clearer prohibition in the Constitution of the government doing that- I mean, the Fifth Amendment is about as clear as can be. No one shall be subjected to the loss of life, liberty, or property without due process.
What has happened is the government has succeeded in terrifying Americans into believing that if they sacrificed their liberties, they would somehow be safer. Yet, some of the things that George W. Bush did on his own have been done by Barack Obama and he has used the fact that Bush did them and got away with them as a precedent. That troubles me. That fill-in-the-blank that will be elected in 2016 would not be above- unless it's Rand Paul- would not be above or beneath making the same claim and using Barack Obama's unconstitutional, unlawful, and quite candidly, murderous behavior as a precedent. Because when the president does something, and it becomes a precedent – meaning there is no blowback, Congress didn't do anything about it, the courts didn't interfere and prevent it from happening again- when the president gets away with that, it becomes a precedent for a future president. Basically, in amending the Constitution by consent rather than by amendment, by agreeing to overlook the due process clause when the president wants to, this would turn Jefferson and Madison and even Hamilton and Adams in their graves!"
Further speaking on the lengths in which Obama has allowed for military-like police to operate here in the United States – speaking in particular about officers locking down entire communities in search of the "Boston Bombers" – Napolitano continued to say destroy Obama's "totalitarian and unconstitutional," mindset:
You know, my colleagues in the media, including my colleagues here at Fox, use a standard phrase when that happens – and I loathe that phrase because the phrase comes from the operation of a prison- and the phrase is 'lockdown.' 'Watertown, Massachusetts, is on lockdown.' Well, Watertown, Massachusetts, is a town, a free town in America! The government cannot lock anybody down. Yet, we use that phrase."
After you've listened to the entirety of the interview, feel free to leave us your thoughts on what Napolitano had to say in the comments below.
(h/t: TPNN)


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At Nationwide Children's Hospital, patients, families and visitors to the Emergency Department will be screened by our security officers prior to entering the ED. What does this involve?
Our goal is to identify and remove items that could be used as weapons. Should the security officers need to remove any items, those items will be held during the patient and families' stay and available for pick-up later. Due to the lack of storage, items can only be held for seven days and then they will be discarded. We appreciate your patience and help creating the safest possible surroundings for our patients and families.
All patients who come to Nationwide Children's for medical treatment need to have a signed consent for treatment on file. If a child under 18 comes to Nationwide Children's Hospital for treatment with someone other than their parent or legal guardian, we need an authorization from the parent or legal guardian to allow them to sign medical consent for the child. The authorization form names someone over the age of 18, chosen by the parent or legal guardian, to seek medical treatment and sign consent for the child. For example, a baby-sitter, teacher or other family member. Make sure that anyone caring for your child who may need to seek medical attention for your child in your absence, has a completed authorization form. Download the consent form here.


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When you hear the term ?hardcore gamer,? what immediately comes to your mind? Is it yourself or do you conjure up an image of some guy in his room playing twenty-four hours a day?
Remember the South Park episode ?Make Love, Not Warcraft?? In it Stan, Kyle, Kenny, and Cartman play the popular MMO World of Warcraft. As they play they keep getting killed by the number one player in the game. This character is so strong that not even a small army of players can defeat him. And thus our intrepid heroes devote every waking hour to leveling their characters. As they spend all that time playing WoW they start to gain weight until all of them become obese.
?How do you kill that which has no life?"
Now despite the jocular situation one has to wonder if casual gamers and non-gamers alike conjure up a similar image when they hear the term hardcore gamer. If so then it looks like they might have to change that particular mental conjuration according to a study in the Journal of Communications.
Taken from this article:
Scientists conducted a survey of some 7,000 players who were logged on to a game called EverQuest II. And they discovered some interesting things. First off, the average age of the gamers surveyed was 31. And that playing time tended to increase with age. Which is also where the sex differences come in. The female gamers actually logged more time online: an average of 29 hours a week, versus 25 for the males, with the top players putting in 57 hours a week on the girl?s side, and 51 for the guys. What?s more, it looks like women are more likely to lie about how much they really play. The researchers found that the gals tended to lowball how long they spend glued to the screen.
Meaning no offense to those who did this study but I would be curious to see if they would have gotten the same results had they surveyed an equal number of people who played World of Warcraft. I could see the average age of an Everquest player to be higher than the average age of a WoW player. After all Everquest, the first game, was out a lot longer then WoW. It only makes sense that the same gamers, who played Everquest, would be playing Everquest II whereas WoW has attracted both veteran gamers and a new generation.
From my own personal experience of when I was in a WoW guild, on the Bleeding Hollow server, I know that the majority of our members were in their mid twenties while we had several who were considerably younger and only a couple that were older. I would venture to guess that the average age of a WoW player would probably fall around 25-27 years of age. But of course I don?t have any proof to back this up.
Still their results are pretty fascinating and, to me, surprising. I never would have thought that female gamers would shoot past us guys in the length of playing. My hats off to you ladies. You are more hardcore than us men when it comes to Everquest II.
But what do you think of these results? Do you think they are accurate and indicative of the gaming world today? Would these results apply to all the new MMOs that have come out over the past five years?
You know, I?ve never eaten a hot pocket before. I guess I?m just not hardcore?


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Victoria's Ghosts
We are on the hunt for more iconic Victoria ghosts and the stories behind them.
The Doppelganger of Beacon Hill Park
A woman was spotted, arms outstretched, face to the sky, on a large rock outcropping in the corner of Beacon Hill Park closest to St Ann's Academy. She never acknowledged her friends as they passed by, and when these friends mentioned seeing her later on in the day, the woman would deny being anywhere near the rock. In fact, she often had firm alibis to the opposite.
One morning, the woman went missing. She was found at the base of the rock, murdered. This woman's death is a long standing, unsolved case in Victoria.
(Doppelgangers are known to be omens of death - a portion of your spirit arriving ahead of you, which, when reunited with the rest of your soul, leads to your death.)
Ross Bay Cemetery Ghosts
Ross Bay Cemetery is the oldest surviving formal landscape design in B.C. and is a superb example of a Victorian-era burial ground. The cemetery is noted for several resident ghosts, including David Fee (who was murdered on the steps of St. Andrews Cathedral on Christmas Eve 1890), Isabella Ross (the first woman in British Columbia to own land, whose farm stood where the cemetery is now) and a mysterious, elderly couple who are dressed in fancy Victorian attire and who are seen from time to time gliding along the western side of the cemetery.
Spirits at St. Ann's Academy
Deeply concerned about what would happen to the school they erected, the original sisters of St. Ann's Academy couldn't leave and have been spotted in front of the building with troubled expressions. They had seen the building fall into disrepair after they had moved out and to this day don't believe that the honour of housing the Ministry of Education will last. It was also rumored that Thomas Hooper, St. Ann's architect, killed people and had them poured into the foundations to give his buildings "souls." When taking a stroll past the buildings, their presence is undoubtable.
Bastion Square's Eternal Inmates
Haunted by its dark history, Bastion Square is home to the ghosts of prisoners who found their neck in a noose, or who were tossed into the jail's graveyard. Before the jail was demolished in 1885, one of the prisoners died in Helmcken Alley after a beating from a guard. To this day, a man in old prison attire with chains shackled to his wrists and ankles follows visitors and locals down the alley with heavy footsteps. The spirits of some of his old cell mates are still trapped in the Maritime Museum of British Columbia, considered British Columbia's most haunted building.


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We are about to witness a huge drama – one that will, ultimately, determine the fate of the United States of America…and the whole human race.
Before us is a clear and present danger. There is no doubt about it. Anyone who cares to look can see it. If something is not done to protect ourselves against it, the results will be either disastrous or fatal…we're not sure which.
This week, Addison's movie I.O.U.S.A. opens in 358 theatres across the nation. It is not merely a documentary film…it is also an expression of hope for our species. The question at hand – the real theme of this monumental drama – is whether a group of people can change the course of history.
You see, dear reader, the U.S. is in a fix. Nothing unusual about it – nations turn into empires (when they are unlucky)…then, they overstretch…they overspend…they overdo it. According to former U.S. comptroller – and the star of I.O.U.S.A. – David Walker, the U.S. government was in over its head by more than $50 trillion in 2007. Unless action is taken, the country will go broke.
Everybody knows that is true. But so far nothing has been done to correct the situation. Too many people in too many positions of power – including the lumpen voter himself – have too many reasons to want the system to continue unchanged. These people are getting something for nothing – and hoping to put the costs onto the next generation. But if the expenses continue to build up…the whole mountain of debt will come tumbling down.
Your editor is proud to have contributed to the movie. It was based, originally, on a book he wrote, with Addison, Empire of Debt. And he has a small speaking role in the film itself.
"I like what you say in the movie," says a friend who saw the preview. "But you look terrible. You look like a pervert."
In the film, we point out that empires always overstretch…and always collapse. Looking at history, we see that this story usually plays out as a tragedy – usually accompanied by defeat on the battlefield and national bankruptcy. Nevertheless, the Peterson Foundation is spending a billion dollars, according to press reports, to try to give our own "Made in the U.S.A." version of this old tale a happy ending. If people can be made to understand the problem, they will do the right thing…or so they believe.
And there is the question: can right-minded, well-meaning people really change the course of history? Is that the way it works…?
We will see, dear reader…we will see.
*** Meanwhile, in Lindau, Germany, a group of 14 Nobel Laureate economists have gathered to discuss the world's problems. Myron Scholes, who won his Nobel for his work on derivative pricing, said the financial crisis was "not over and I'm not exactly sure when it's going to be over." There will be "lots of business failures," he went on.
Joseph Stiglitz described the cause of the problem as a "massive failure of the brains of the economy."
Nobody was impolite enough to mention that many of the biggest brains were in the room…and that they had created many of the bubble's biggest delusions.
*** Our party ended last night about 3 AM.
"This whole place is like a giant pile of tinder…and everyone in the room has a match…" said Elizabeth. More to come…
Bill Bonner
for Markets and Money


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Spencer School207 Westfield StreetMiddletown, CT 06457Phone: 860-344-0711Fax: 860-344-0490
Principal:Suzanne firstname.lastname@example.com
To report an absence:860-344-0490
Our Adopt-a-SchoolBoard of Ed. member is:Christopher R. firstname.lastname@example.com
Identifying Children and Responding Early
VISION: That all Middletown children will enjoy optimal emotional and behavioral health, and that both children and families will receive the support they need to make this possible.
MISSION: The early identification of behavioral health issues in elementary age children who may benefit from the provision of evidence-based, culturally responsive early interventions in three settings: school, home, and community.
Children who participate in iCARE have improved overall functioning and reduced severity of behavioral problems as measured by the Ohio Scales. Both teachers and parents report high satisfaction with the initiative's efforts. Teachers have rated iCARE students as being less depressed, having fewer problematic behaviors, better attendance, improved self worth, better interpersonal relationships, better self-direction, motivation and overall well-being.
BACKGROUNDThe three key partners in the iCARE initiative are Middlesex Hospital Center for Behavioral Health, Family Advocacy Program, Middletown Public Schools, and the Ministerial Alliance, which is an association of 23 African American churches. iCARE currently operates at two pilot sites: Bielefield and Spencer elementary schools in Middletown, CT.
The iCARE initiative, currently in its final year of funding from the CT Health Foundation, was developed by the Middletown community for two primary reasons: 1) behavioral issues that begin in elementary school, without adequate intervention, many times continue into the later years, and 2) children of color are less likely to get needed mental health services and more likely to become involved in the juvenile justice system later in life.
HOW DOES iCARE ASSESS AND INTERVENE?:
— A Universal Screen administered at the start of each school year to identify at-risk students. (Children may also be referred into the iCARE system by school staff, family, Ministerial Alliance, primary care provider, or other service provider.)
— Regular use of the School Climate Data Team by the three partners as a communications hub to provide a coordinated approach to data analysis (e.g., attendance, discipline, universal screen), early identification of issues, provision of school- and community-based interventions, and review of student and family progress.
— The Talking and Learning Center (TLC) for in-school responses. The iCARE's TLC Interventionist helps to de-escalate crises, assists students with problem solving and conflict resolution, and increases students' pro-social and self-regulation skills.
— Linkage to community resources for families through a Wraparound approach provided through iCARE's Services Coordinator (LCSW) and Community Interventionists, who work together to engage families in creating a strength-based, culturally-relevant action plan and connecting them to the services, resources and natural supports they need. Follow up is provided to see if progress is being made or if other interventions are needed.
— Building trust between the family and school and between the family and behavioral health provider. Research has found that the lack of trust is a major reason why families do not seek assessment and early intervention for their children. Both the Services Coordinator and Community Interventionists are charged with this important task.
Any links to non Middletown Public School District information are provided as a courtesy. They are not intended to constitute an endorsement of the linked materials by the Middletown Public Schools, the Superintendent of Schools, or Board of Education.STATEMENT OF NON-DISCRIMINATION --The Middletown Board of Education is an affirmative action/equal opportunity employer, and it does not discriminate on the basis of race, color, religious creed, age, marital status, civil union, military or veteran status, national origin, ancestry, sex, gender identity or expression, sexual orientation, or past or present physical or mental disability in any of its education programs, activities, or employment policies. All educational programs and offerings, including vocational education and extracurricular activities, subscribe to this policy. Marco Gaylord, Director of Operations, has been designated to handle inquiries regarding the district non-discrimination policies and may be reached at 311 Hunting Hill Avenue, Middletown, CT 06457; phone (860) 638-1485.


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Using Jedi mind trick to get up in the morning
January 9, 2012 Leave a comment
When you are the parent of an 8-month-old sleep is the last thing you get to do. And getting up in the morning can be harder than ever before.Luckily I have a unique trick that helps me get up and start my day.
As I get into bed I actually tell myself, "I'm going to get up, get out of bed and be in a good mood."
In a way I am preparing myself for the inevitable and undulating task of waking up, especially when I work evenings and only get five to six hours of sleep.
The next morning my wife wakes me so I can take care of baby Jaydon and she can head off to work. A little groggy, the words I said to myself come back to me and the next thing I know I am out of bed. Within a couple of minutes I am feeling good and awake.
I've actually used this trick hundreds of times in the past even before we had Jaydon.
Another trick that surprises me and my wife every time is setting a mental alarm clock. I tell myself I want to get up at certain time, for example 6:00 a.m. and sure enough I wake up within ten minutes of 6:00. It's gotten to the point where I do not even have to set an alarm. Although if I have to get up for something very important I will set a conventional alarm.
Now this does not mean I can go indefinitely on five to six hours of sleep. I do drink coffee usually about a hour after getting up and take naps when Jaydon does.
I'm not a follower of holistic or dharma beliefs but I have discovered that a little Jedi mind control helps me get up in the morning so I can start the day with my son.


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No approved endorsements
About Mr. Diarmuid Kelly
Professional with a degree in computing science with extensive international experience in the telecommunications industry in areas of Business Development, Strategic Sales & Marketing, Software R&D Product and Project Management as well as Quality Assurance.
Dynamic, results oriented and highly motivated with strong leadership qualities. Thoroughly versed in all aspects of business acceleration and experience in business turn around with competent negotiating skills at all levels of management and proficiency in multi-cultural business environments and cross-cultural communications.
If you notice offensive or incorrect information, please contact us.


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Login to keep track of your entries.
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All you need to do to enter is provide your email address.
To increase your chances of winning, share the contest on social media! You will receive 1 bonus entry for every friend that enters the contest through your post.
The winner will be announced on Sunday, October 9th.
Eligibility and Prize Information
ELIGIBILITY: Open to all.
GRAND PRIZE: 1 full collection of cufflinks bearing images and characters from the upcoming film, Rogue One: A Star Wars Story including 1 pair each of cufflinks with images of the Death Star blueprint plans, K-2SO, a TIE Striker, a Death Trooper, K-2SO in 3D, a TIE Striker in 3D, and a Death Trooper in 3D.
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Our organization is positioned at Jaipur(India) and has gained immense acknowledgement and a respected place in the market. We have a team of expert quality analysts that stringently checks each and every stage for ensuring quality products at buyer's end.
We are Exporter and Supplier of Fresh Potato from Rajasthan, India. These are procured from our experienced famers who cultivate them under suitable conditions without using any harmful pesticides. We maintain a large stock in our warehouse to meet the urgent requirements of the customers. Our quality control experts test these potatoes at various parameters prior to dispatch. Due to our large distribution network, w
Harsh Priya Export
No. 116, Evershine Tower, Vaishali Nagar, Rajasthan - 302021, India
We are Leading Exporter & Supplier of all Kind of Fruits & Vegetables from India to worldwide. We are in export of : Potato Onion Garlic Ginger Tomato Apple Papaya Mango BananaLemon Pineapple Orange Grapes Water Melon Cherry Coconut If you are looking to Buy quality Fruits & Vegetables from India then please contact us to know more details from us.
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M Krishna Import Export Pvt Ltd
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About Us
Clare Burnett PRBS
Clare Burnett is a site-responsive artist based in London. She makes pared-down, abstract artworks which encourage people to engage visually and conceptually with their surroundings.Previous projects include installations of works in West Norwood and Brompton Cemeteries, street works and paintings in Le Corbusier's Unite d'Habitation. Last year Clare was artist-in-residence at Leighton House Museum where she had a major solo show. She is President of the Royal British Society of Sculptors.
Vice President
Julian Wild VPRBS
Julian Wild graduated from Kingston University in 1995. His work explores the relationship between drawing and sculpture through the use of a wide variety of materials and surface finishes. He has been commissioned to make sculptures for organisations such as Cass Sculpture Foundation, Jerwood Sculpture Park and Fidelity Investments. He has held solo exhibitions at Canary Wharf, William Benington Gallery, Burghley House, Maddox Arts, Leighton House Museum, Spitalfields Square and Modern Art Oxford. He was a finalist for The Jerwood Sculpture Prize in 2005 and won The Millfield Sculpture Prize in the same year. In 2009 he was awarded the 3 year Chelsea Arts Club Trust, Royal Borough of Kensington and Chelsea Studio Bursary. He runs the touring communal sculpture project Making the Connection and was the founding member of ForumArts.
"Being a member of the RBS has benefited my practice enormously. I have made great friends and contacts through the organisation. The RBS facilitates an exchange of ideas and promotes discussion between its members. I would like to see its membership grow and to see the RBS continue to offer its members professional development support and exciting opportunities"
Matthew French - Treasurer
Matt has been a corporate finance specialist in the City since graduating from Cambridge. He currently works as a freelance, non-executive company director. Prior to this he worked as an investment banker with Nomura and UBS, after a long career in private practice as a solicitor.
Katherine Ashton
Kate Ashton is a corporate solicitor at Debevoise & Plimpton LLP with a broad international practice, including extensive experience in private equity transactions. Originally from the U.S., Kate has lived in England for more than 20 years. She is a member of the Board of Trustees of the American Trust for the British Library and is active in a number of other cultural and pro bono organisations. Kate co-founded the Lawyers Volunteering for the Arts pro bono initiative in London, through which a consortium of law firms provides pro bono legal advice to low-income arts and cultural organisations in the UK, and serves on its Executive Committee. She is a graduate of Harvard College and Harvard Law School. She has been involved with the RBS since 2009, when she advised on a pro bono basis as to a major revision of its articles and corporate governance structure.
Alex Chinneck MRBS
Bruce Gernand FRBS
Bruce has spent many years teaching, primarily at Central Saint Martins College of Art and Design. He has continued with research and commission projects and still enjoys working in his studio.
Anderson Inge FRBS
Having joined the RBS 15 years ago, Anderson became a Fellow in 2014, and was elected to Council in 2015. He has had 2 collaborative shows in the RBS Gallery, in 2004 and 2008, both with RBS Fellow Bruce Gernand.
Anderson is a formal migrant, having travelled from architecture through structural engineering to arrive at sculpture. Anderson initially studied architecture at the University of Texas at Austin and the Architectural Association (AA) in London. He then completed post-graduate degrees in architecture and structures concurrently at M.I.T.
His training in sculpture at Central Saint Martins much later as a mature student made sense of the journey, and it now underpins his practice and teaching of all 3. With his training in architecture, Anderson tries in particular to assist the RBS Council with its evolving plans for the RBS premises.
Matthew Jarratt
Matthew is based in Gateshead, and works across the UK and China on arts commissioning, curatorial projects, and cultural policy. Matthew spent 15 years at Arts Council England as a creative broker helping to shape the North East culture led regeneration, developing many new arts buildings and artist's projects across the region. Matthew has worked on city arts strategies, major capital projects, and funding at a senior level with the private sector, local authorities, universities , politicians, and cultural leaders in addition to working on European funding for Arts Council England in Brussels.
Matthew has curated and managed over 100 sculpture commissions within public and private sector developments. Directing the public art unit Commissions North which brokered many artists projects including Anish Kapoor's Temenos sculpture in Middlesbrough.
In 2013 Matthew began his freelance cultural consultancy working in partnership with Joanna Riddell to create Cheeseburn - a new sculpture park and gallery within her historic house and estate near Stamfordham in Northumberland.
In addition Matthew has a senior policy role as manager of the North East Culture Partnership www.case4culture.co.uk which works across local authorities, universities, and the business sector to champion the value of arts and heritage.
Matthew is a regular visitor to China curating exhibitions, developing artists projects and brokering opportunities between Chinese and international museums. www.matthewjarratt.com/cn/current
Briony Marshall MRBS
Briony Marshall is a London based sculptor and installation artist. With a background in Biochemistry she is interested in the idea of art practice as research. She fuses an intellectual/conceptual approach with an intuitive, materials inspired process to develop works that investigate the natural world and man's place in it.
In 2012 she completed the Pangolin London Sculpture Residency which culminated in a solo show with the gallery. She is head of Professional Development at The Art Academy and was recently named as one of the '175 Face of Chemistry' to celebrate the 175th anniversary of the Royal Society of Chemistry.
Briony was selected for the Royal Society of British Sculptors' Bursary Award in 2008, and later went on to receive the 2010 Brian Mercer Bronze Casting Residency and was a finalist in the 2015 First@108 Public Art Award. Having benefited so much from the support of the society she has been keen to give back through various projects including facilitating peer mentoring sessions for the new bursary awardees since 2013.
Alice Cunningham MRBS
Alice Cunningham's practice ranges from community led art works, performance, video, sound, collaborating with landscape architects making public art, to painting and stone carving. She is often inspired by the landscape and social or environmental issues.
Since completing her first Fine Art sculpture degree at Brighton University in 2006, she has exhibited throughout the UK, Europe, Asia, and Africa. In 2010 she was shortlisted to represent the UK in the first UK Young Artists Biennale in Europe and has lectured in Fine Art at Universities throughout the UK. Alice has also worked with charities organising creative events to engage hard to reach groups.
Alice has been a member of the RBS since receiving a bursary in 2007 and was awarded the RBS Brian Mercer stone carving residency in 2014. "I have met some fantastic artists and friends through the network and have felt part of a supportive and professional organisation leading by example of the important role of the artist in society. I am keen to help shape the society's continuation and growth."
Carlo Rizzo
Carlo had been a Trustee of the Royal British Society of Sculptors since summer 2015. He is the founding director and programme curator of the Exhibition Road Commission, a biennial interdisciplinary programme and artist commission co-produced by all the major museums and universities based in South Kensington. He runs an independent advisory practice helping public and private organisations in London develop cultural strategies and new interdisciplinary partnerships. Carlo is also a non-executive director of Goldfinger Factory, a social enterprise training people in need of professional development to produce furniture from waste material, and a researcher at the Victoria & Albert Museum where he is undertaking a PhD on the museum's collection of international contemporary art and design.
Prior to launching the Exhibition Road Commission, Carlo was a member of the Science Museum management team and worked on several strategic projects including setting up the Science Museum Foundation, and the redevelopment of the Mathematics Gallery and Medicine Galleries. Prior to the Science Museum, he worked in the department of the Near and Middle East at the British Museum, supporting the development of their contemporary art collection. Carlo's career started in financial services, with roles at the Bank of England and as a strategy consultant in several financial institutions across the UK and Europe.
Andrew Stonyer FRBS
Stonyer studied at Northampton School of Art, Loughborough College of Art, The Architectural Association and Leicester Polytechnic/Slade School of Fine Art. In 1978 he was awarded the first practice based PhD in Sculpture in the UK, through his studies at Leicester Polytechnic and the Slade School of Fine Art. His PhD focused on the use of solar energy in kinetic sculpture.
Andrew has taught, exhibited and completed commissions in Turkey, Holland, Canada and the United States of America, and his work can be found in public and private collections in all of these countries. From 1994-2010 he was Professor of Fine Art at the University of Gloucestershire. Other academic appointments during this period included Visiting Professor in Fine Art at Mimar Sinan University in Istanbul and Visiting Fellow in the Faculty of Engineering at Bristol University. He is a Trustee and former Chairman of the Forest of Dean Sculpture Trail and a member of the Fabric Committees at both Gloucester and Worcester Cathedrals.
His sculpture is a search for patterns of actual and implied kinetic imagery that are hidden within the seemingly regular, and are always transcribed through the geometry of the circle and the square such as his Rotating Circle.
Andrew joined the RBS as an Associate member in 2005, was elected a Fellow in 2008 and elected to Council in 2014. He exhibited in 'Kinetic' at the RBS in 2009 and in 2010 in the solo exhibition 'Timepiece'.


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CRICOS Course Code: 092265B
Gain the skills and knowledge to pursue a career in the security industry. This course is suitable for students who are interested in obtaining the Security Officer License from the WA Police, which enables you to work as Security Officer in Western Australia. You will learn to respond to security risk situations, protect yourself and others using basic defensive tactics and how to apply first aid.
Security Officer*:
This course is delivered over 2 weeks (80 hours)
Security Officer + Crowd Controller Combined*:
This course is delivered over 3 weeks (112 hours)
*Classes are rostered from Monday to Friday, 8.00am to 4.30pm
This qualification suits students seeking employment in the following job role:
The delivery methods for this course include:
Recognition is available (on application) to students with prior skills, experience, knowledge or qualifications obtained from formal studies or training, in a related area. Recognition may reduce the duration of your studies.
Cluster 1: CPP20212 Certificate II in Security Operations
Students must successfully complete the following units in order to complete their CPP20212 Certificate II in Security Operations:
Cluster 2: Crowd Controller Pathway
To obtain employment as a Crowd Controller in licensed premises and places of entertainment, you may elect to complete the following 4 additional units:
Security Officer:
Fee: $600
Security Officer + Crowd Controller Combined:
Fee: $800
Weekly intakes
Completion of Australian Year 10 or equivalent
Students are also advised that whilst Stanley College has no pre-requisite requirements for entry to this course, in order to apply for a license with WA Police, students are required to be at least 18 years old.
*Applicants must obtain a National Police Clearance during the licencing application process
Private security activities within Western Australia are governed by the Security and Related Activities (Control) Act 1996 and the Security and Related Activities (Control) Regulations 1997 managed by the Western Australian Police Licensing Services. To work as a Security Guard and / or Crowd Controller, you are required to obtain the relevant licences, with one requirement being that you have completed an approved training course with an approved training provider. Stanley College's Certificate II in Security Operations is registered on the list of Security Training Providers approved by the Commissioner of Police. Licensing Update - Security Officer and Crowd Controller licence applicants - From 1 February 2017, due to amendments to the Security and Related Activities (Control) Regulations 1997, all applicants for Security Officer and/or Crowd Controller licences, must have successfully completed a written examination based on the content of their training course before submitting their application.Full details on the Licensing Requirements and relevant application forms can be found at http://www.police.wa.gov.au/. Full details on competency testing and relevant booking/payment information can be found at http://www.saiwastc.com.au/
To apply, click here to fill in our application form.


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When it's late and you want a pick-me-up, a hyper-caffeinated energy drink might appeal more than an espresso. But their makers face a serious setback, as the United States' Food and Drug Agency (FDA) is investigating links between Monster, which contains three times more caffeine than an 8-ounce can of Red Bull, and the deaths of five people in the last year.
Reports were released under the Freedom of Information Act after the mother of one Maryland teenager filed a lawsuit against Monster, alleging that her daughter died after drinking two large cans of the hyper-caffeinated beverage. The official autopsy said the cause of death was "cardiac arrhythmia due to caffeine toxicity".
Monster denies any blame, saying the company has sold 8 billion energy drinks over the past 16 years, with no proof tying them to any fatalities. Energy drinks are currently allowed higher levels of caffeine as they are sold as dietary supplements, not sodas.
But US Senator Dick Durbin wants to limit these levels because emergency room visits related to energy drinks soared 10-fold between 2005 and 2009 - although half of these involved drugs or alcohol too.Reuse content


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by Zehui
Order now to get it by: Sunday July 09 - Tuesday July 11
This product ships with limited tracking information, and may take longer than usual to be delivered.
Condition: New
Product ID: 220918
Delivery Information |Returns & Exchanges |Payment Methods
M: 25.2-27.6 inch;
L: 27.6-30 inch;
XL: 30-32.4 inch
Kind reminder: Asia sizes is smaller than US/AU/UK size so Please make sure of these actual measurements will fit you.
Item Includes:1* Men's Trunks


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| Recently found by viaLibri....
Daido Hysteric no.4, no.6, and no.8 [3 VOLS. COMPLETE SET WITH THE SHIPPING BOXES]
Tokyo: Nobuhiko Kitamura / Hysteric Glamour, 1993-1997 - Daido Hysteric no.4 (Tokyo). Tokyo: Nobuhiko Kitamura / Hysteric Glamour, 1993. Square folio (364 × 314 mm), pp.[352]. Black-and-white photographs. 346 black-and-white photographs. Art direction by Osamu Wataya and Kimie Hata. Black-and-white photo-illustrated perfect bound wrappers, band on upper side printed blue, text in black; handling marks, light rubbing and wear, crease to front hinge. Publishers lightly worn shipping carton with Hysteric Glamour printed tape. A very good copy. [With:] Daido Hysteric no.6 (Tokyo). Tokyo: Nobuhiko Kitamura / Hysteric Glamour, (1994). Folio (364 × 314 mm), pp.[354]. 174 black-and-white photographs. Art direction by Osamu Wataya and Toshio Shiratani. Black-and-white photo-illustrated perfect bound wrappers, band on upper side printed purple, text in black; light wear to edges. Publishers lightly worn shipping carton marked by tape removal. Near-fine. [And:] Daido Hysteric no.8 (Osaka). Tokyo: Nobuhiko Kitamura / Hysteric Glamour, (1997). Folio (363 × 314 mm), pp.[408]. 373 black-and-white photographs. Art direction by Osamu Wataya, Toshio Shiratani and Kiyokazu Tanno. Printed perfect bound wrappers, red, text in white; light endemic wear to spine and edges, spine and page edges lightly sunned. Moriyamas signature in black ink to title-page. Publishers lightly worn shipping carton with remnants of Hysteric Glamour printed tape. An excellent copy. First editions, no.4 and no.6 limited to 300 copies and 600 copies respectively, all with the often discarded shipping cartons, no.8 signed by Daido Moriyama. These three books, produced in collaboration with innovative fashion label Hysteric Glamour, signalled a new, less lyrical direction in Moriyamas work. The first two contain photographs of Tokyo, while for the third Moriyama took photographs in Osaka, his hometown. The following conversation is between Osamu Wataya, art director of these three books; Hajime Sawatari, photographer; and Michitaka Ota, editor and president of Sokyu-sha. Ota: You know theres not much left to say about Mr. Moriyama now, is there? Sawatari: Mmm, anyone whos anyone has already talked and written about him so much. Ota: And yet none of them make any sense to me though! Sawatari: Yeah, thats right. Even reading them carefully, I still cant understand them. (Laughing) Ota: But you know, once you see his work . it all becomes very clear. Sawatari: Yeah, you either love it or hate it. Wataya: And theres no particular reason why its good or bad. Sawatari: Yes, the good ones are good, others are very cool, and the rest are . different. So I think its enough to just say. Mr. Moriyama is really cool. Or not? (Laughing) Wataya: (Laughing) I agree. [Attributes: First Edition; Soft Cover]
[Bookseller: Oliver J. Wood]
Last Found On: 2016-11-27 Check availability: AbeBooks
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Christmas in July Brisbane
Published: Monday 30th June 2014
You are invited to the Brisbane Campus's Staff Club's Christmas in July celebrations
The celebration is being held on Thursday 10 July in NA.06/07 (FLA) at 12pm.
This event is free to members or $25.00 for non-members.
Come and enjoy the fun, good food, door prizes and great fellowship.
Please RSVP to Kris Burrows firstname.lastname@example.com by Friday 4 July.


text: HATFIELD, England, March 28, 2013 /PRNewswire/ -- If you're looking for a smart, reliable handset with the benefits of a superfast network, EE could have the answer. They will be the first and only UK network to launch the new Samsung Galaxy S4 with 4G and will provide customers with a range of cost-effective 4G data bundles. As well as fast, efficient connections times and speeds of up to five times faster than 3G, consumers can enjoy a range of additional services. Those with a 4GEE payment plan can watch live TV on the move without buffering, play multiplayer games on the go and download large email attachments - without having to wait around. Benefits also include an exclusive EE film package, monthly discounts on EE Superfast Fibre Broadband, a fast track customer service helpline, 500MB of storage and plenty of add-ons for those who opt for payment plans from £41 a month. The sleek, lightweight handset will be available online, via EE telesales and in EE stores from the 26 th April and will also operate through Orange and T-Mobile's 3G network. According to Paul Jevons, Director of Products and Devices for EE, the new Samsung Galaxy is the perfect addition to the 4G range. He said: "When paired with our unique superfast 4G network, customers really will be able to make the most from the powerhouse performance the Samsung Galaxy S4 provides - utilising its capabilities to the maximum." Since the 4G network launched last year, it has already been rolled out to 37 town and cities and is set to arrive in 35 more by June - bringing the total up to 72. The network now covers the homes and businesses of more than 45 per cent of the UK population and has improved the quality of communication significantly. Customer Services Hatfield Business Park UK AL10 9BW Telephone number: +44(0)800-956-6000 E-Mail: firstname.lastname@example.com Website: ee.co.uk SOURCE EE


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- Patient Comments: Down Syndrome - Symptoms
- Patient Comments: Down Syndrome - Diagnosis
- Patient Comments: Down Syndrome - Experience
- What is Down syndrome?
- What causes Down syndrome?
- What are the signs and symptoms of Down syndrome?
- How is Down syndrome diagnosed?
- What is the treatment for Down syndrome?
- What are the potential complications of Down syndrome?
- What is the prognosis for someone with Down syndrome?
- What is the life expectancy for someone with Down syndrome?
- Can Down syndrome be prevented?
What is the prognosis for someone with Down syndrome?
With increased understanding of potential underlying illnesses associated with Down syndrome and with increased support within the community for patient and family, quality of life for the patient has increased in recent years. There is great variability in mental function between patients, but for those with the appropriate mental capacity, the ability to complete an education, live, and work independently.
Down syndrome patients tend to age more quickly and are prone to developing Alzheimer's disease, often as young as age 40. Those patients who maintain their mental capacity tend to have a longer life span. A poorer prognosis is found in those patients who develop dementia, lose their ability to perform daily physical activities, and those who have decreased vision.
What is the life expectancy for someone with Down syndrome?
Because of the many physical complications associated with chromosomal abnormalities, many fetuses with trisomy 21 fail to develop properly and are miscarried. There is an increased risk of infant death in the first year of life because of congenital heart disease (failure of the heart to develop normally) and respiratory infections.
Given the challenges in the infant years, patients with Down syndrome have experienced increased life expectancy as recognition and treatment of underlying medical conditions improve. A person with Down syndrome can expect a life span of 50 years and more, depending upon the severity of underlying medical issues.


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Product Pathways - Cell Cycle / Checkpoint
PathScan® Phospho-Chk1 (Ser345) Sandwich ELISA Kit #67625
PhosphoSitePlus ® protein, site, and accession data: CHK1
|Product Includes
|Solution Color
|ELISA Sample Diluent
|25 ml
|Anti-rabbit IgG, HRP-linked Antibody (ELISA Formulated)
|1 ea
|Red (Lyophilized)
|Detection Antibody Diluent
|11 ml
|HRP Diluent
|11 ml
|Sealing Tape
|1 ea
|STOP Solution #7002
|11 ml
|TMB Substrate #7004
|11 ml
|Chk1 Mouse mAb Coated Microwells
|96 tests
|Phospho-Chk1 Ser345 Rabbit Detection mAb
|1 ea
|ELISA Wash Buffer (20X)
|25 ml
|Cell Lysis Buffer (10X) #9803
|15 ml
Specificity / Sensitivity
PathScan® Phospho-Chk1 (Ser345) Sandwich ELISA Kit detects endogenous levels of Chk1 protein when phosphorylated at Ser345, as shown in Figure 1.
The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
The PathScan® Phospho-Chk1 (Ser345) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Chk1 when phosphorylated at Ser345. A Chk1 Mouse Antibody has been coated onto the microwells. After incubation with cell lysates, Chk1 (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a Phospho-Chk1 (Ser345) Rabbit Detection Antibody is added to detect phosphorylation of Ser345 on the captured Chk1 protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of Chk1 phosphorylated at Ser345.
Antibodies in kit are custom formulations specific to the kit.
ELISA - Western correlation
Figure 1. Treatment of HeLa cells with UV stimulates phosphorylation of Chk1 at Ser345, as detected by the PathScan® Phospho-Chk1 (Ser345) Sandwich ELISA Kit, but does not affect the levels of total Chk1 detected by the PathScan® Total Chk1 Sandwich ELISA Kit #7872. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Chk1 Antibody #2345 (left panel) or Phospho-Chk1 (Ser345) Rabbit mAb #2348 (right panel) are shown in the bottom figure.
Figure 2. The relationship between the protein concentration of lysates from untreated and UV-treated HeLa cells and the absorbance at 450 nm using the PathScan® Phospho-Chk1 (Ser345) Sandwich ELISA Kit is shown. HeLa cells (80-90% confluent) were treated with 100 mJ/cm2 UV with 1 hour recovery at 37ºC and then lysed with the lysis buffer.
Chk1 kinase acts downstream of ATM/ATR kinase and plays an important role in DNA damage checkpoint control, embryonic development, and tumor suppression (1). Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. Activation occurs in response to blocked DNA replication and certain forms of genotoxic stress (2). While phosphorylation at Ser345 serves to localize Chk1 to the nucleus following checkpoint activation (3), phosphorylation at Ser317 along with site-specific phosphorylation of PTEN allows for re-entry into the cell cycle following stalled DNA replication (4). Chk1 exerts its checkpoint mechanism on the cell cycle, in part, by regulating the cdc25 family of phosphatases. Chk1 phosphorylation of cdc25A targets it for proteolysis and inhibits its activity through 14-3-3 binding (5). Activated Chk1 can inactivate cdc25C via phosphorylation at Ser216, blocking the activation of cdc2 and transition into mitosis (6). Centrosomal Chk1 has been shown to phosphorylate cdc25B and inhibit its activation of CDK1-cyclin B1, thereby abrogating mitotic spindle formation and chromatin condensation (7). Furthermore, Chk1 plays a role in spindle checkpoint function through regulation of aurora B and BubR1 (8). Research studies have implicated Chk1 as a drug target for cancer therapy as its inhibition leads to cell death in many cancer cell lines (9).
- Liu, Q. et al. (2000) Genes Dev 14, 1448-59.
- Zhao, H. and Piwnica-Worms, H. (2001) Mol Cell Biol 21, 4129-39.
- Jiang, K. et al. (2003) J Biol Chem 278, 25207-17.
- Martin, S.A. and Ouchi, T. (2008) Mol Cancer Ther 7, 2509-16.
- Chen, M.S. et al. (2003) Mol Cell Biol 23, 7488-97.
- Zeng, Y. et al. (1998) Nature 395, 507-10.
- Löffler, H. et al. (2006) Cell Cycle 5, 2543-7.
- Zachos, G. et al. (2007) Dev Cell 12, 247-60.
- Garber, K. (2005) J Natl Cancer Inst 97, 1026-8.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 67625 :
- ELISA Colorimetric (Lyophilized)
Companion Products
- 2348 Phospho-Chk1 (Ser345) (133D3) Rabbit mAb
- 2360 Chk1 (2G1D5) Mouse mAb
- 7002 STOP Solution
- 7004 TMB Substrate
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7870 PathScan® Phospho-Chk1 (Ser317) Sandwich ELISA Kit
- 7872 PathScan® Total Chk1 Sandwich ELISA Kit
- 9803 Cell Lysis Buffer (10X)
- 9808 Phosphate Buffered Saline (PBS-20X)
- 9809 Phosphate Buffered Saline with Tween® 20 (PBST-20X)
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PathScan is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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