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Obama should really think before he speaks (or rather, give orders). This is not amateur hour!
If selected for publication, it will appear as soon as possible on Haaretz.com.
U.S. President Barack Obama condemned Hamas for Friday's reported capture of 2nd Lt. Hadar Goldin, saying if Hamas was serious about resolving the situation the Israeli officer must be freed.
Obama said he condemned both Hamas and the attack within minutes of the incident. "If they (Hamas) are serious about trying to resolve this situation, that soldier needs to be unconditionally released as soon as possible," he told a news conference.
Obama reiterated Israel's right to defend itself, saying no country would tolerate rockets and tunnel attacks. He stated that Hamas is housing rockets in the middle of civilian neighborhoods, "which is why we see innocent people getting killed."
The president said Israel committed itself to the cease-fire before it was violated, cautioning that Israel cannot be confident that a cease-fire will hold if Hamas cannot control all Palestinian factions.
Obama stressed more should be done to protect the lives of innocent civilians in Gaza, saying a cease-fire was one way to do that. "I want to see everything possible done so that Palestinian civilians do not get killed." He also added that, "There is a way of dealing with the tunnels while reducing the bloodshed."
He said that the U.S. remains committed to brokering a truce between the sides. "We are going to continue working on a ceasefire but it will take time and will be hard."


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|Blog Posts
Healthy Chic has a lot of things to help keep you fit and healthy! If you are interested in getting something from them let me know and I can email you a 15% off your first purchase code.
Disclaimer..I was given a $25 dollar credit to their online store in exchange for this review.
2. I recently won a pair of PowerSox on a virtual run I did and then was contacted by the company to see if I wanted to have another pair to review on my blog. Since a girl can never have enough socks I agreed to.
Not sure which I won and which I was sent to review since they came about the same time, but they are both great socks. The first is the Advanced Dry , these are my favorites.
Really lightweight with a little more cushion over the heels and ball of the foot. They are almost mesh like in a few spots and I can see where they will be great for summer running. No irritation from any seams and so far have held up really well to washing.
I haven't run more than 3 miles at a time in these since I got them about the same time I acquired a quad strain, but I have worn them to work all day and had no complaints. A very comfy sock. They fit snug, but don't constrict the toes at all. At 10.99 for two pairs they are a pretty good deal. Right now they are on sale for 8.24 though, so an even better deal if you are in the market for some socks!
The other socks I received are the Gradient Cotton No-Shows, these are considered a women's sport sock.
I had no complaints about these either. Once again, I haven't worn them running much, but I have worn them to work and for working out at home. Very comfy and a little cushier than the Advanced Dry. I think they are an awesome deal on sale right now 8.24 for three pairs.
PowerSox on Facebook Powersox on Twitter
Disclaimer..I won one set of socks and was sent the other in exchange for this review. I was not obligated to give a positive review. All opinions expressed are my own. I was not compensated in any other way.
3. I was also sent Paleo Fitness: Primal Training and Nutrition to Get Lean Strong and Healthy
What's inside
I don't strictly follow a Paleo diet, but I like a lot of their basic principles. Since I have been injured and not running I switched back to a mostly Paleo diet and have seen my skin clear up, and have lost a couple of pounds. I love it.
They promote functional movement and I think that it awesome also. There are some moves in the book I had never done before but will continue to incorporate into my workouts like the clock push up and the salamander press up. They also promote high intensity interval training and I am a HUGE fan of that so that is a big plus in their corner.
I don't think the Paleo diet is for everyone some people get down right fanatical about how could you cut out an entire food group. It is so hard to follow, and when I did it strict I missed the carbs while running so much, but I am not here to argue the merits or downfalls of a paleo diet. This is a great book as a Paleo primer and for workouts. You can connect with them on Facebook
Want to check out the book for yourself? Leave a comment below telling me why you want to try it, or any other random thing about this whole post. I will pick a random winner Monday and post it here. Want to just buy your own copy? Check it out here.
I was sent a copy of this book to review in exchange for a review on my blog. I was not obligated to give a favorable review. All opinions expressed are my own.
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Star Tracks
Kutcher shows off his six-pack while shooting Five Killers in Antibes, France. Plus: Gwen & Zuma, Nicole Kidman and more
Credit: Humberto Carreno/Startraks
Published: Monday Apr 06, 2009 | 05:00 PM EDT
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Flat Water Tuesday
By Ron Irwin; Holter Graham (Read by)
(MacMillan Audio, Compact Disc, 9781427231796)
Publication Date: June 4, 2013
List Price: $39.99*
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Rob Carrey, the son of a working-class cabinet maker, arrives at the Fenton School with a scholarship to row and a chip on his shoulder. Generations of austere Fenton men have led the rowing team, known as the God Four, to countless victories--but none are as important or renowned as the annual Tuesday-afternoon race against their rival, Warwick.
But first Rob must complete months of preparation driven by their captain, Connor Payne's vicious competitive nature. As the race nears, the stakes rise, tempers and lusts are fueled, and no one can prevent the horrible tragedy that befalls one of them.
Fifteen years later, Rob returns home from a film shoot in Africa to end a heartbreaking relationship with his girlfriend, Carolyn. But when a phone call from one of the God Four compels him to attend the reunion at Fenton, no part of Rob's past remains sequestered for long and nothing about his future is certain.
As much about the sport of rowing as it is a novel of finding oneself, not once, but again in mid-life; Ron Irwin's "Flat Water Tuesday" is a testament to the pride and passion of youth, and an ode to the journey of forgiveness.
A stunning novel of boarding school, family secrets, deep and passionate love, and the brutal pain of sports training.
Holter Graham, award-winning audiobook narrator, has appeared in many films, including Fly Away Home, Stephen King s Maximum Overdrive, and Hairspray. His television credits include Army Wives, Wasted, Damages, and Law & Order, among others.


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Over the last 10 years Intronis has transformed the market for cloud based data protection and recovery solutions for small business. Our network of nearly 2000 channel partners service over 35,000 small businesses and we are adding new partners and new end users every day.5/31/2015 Chelmsford, MA Save This Job
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Company Awards
Pinnacle Financial Strategies was named a FIVE STAR: Best in Client Satisfaction Wealth Manager by Crescendo Business Services for 2014. FIVE STAR Wealth Managers represent less than 7 percent of the wealth managers in the Pittsburgh area.
Health Care
Home of the Day
Children's Hospital of Pittsburgh of UPMC has been named one of America's Best Children's Hospitals by U.S. News & World Report. Children's Hospital ranks ninth on the magazine's 2014-15 Honor Roll of America's Best Children's Hospitals. Children's also ranks in each of the 10 pediatrics specialties ranked by U.S. News. This is the fifth consecutive year Children's has been named to U.S. News' Honor Roll.
Legal Services
Tucker Arensberg PC was presented the Outstanding Community Partner Award from Renewal Inc. The award recognizes companies and their employees for their professional and personal support of Renewal's mission.
Seaway Manufacturing Corp. awarded Certified Home Remodelers of Carnegie with a Seaway Award for Sales and Customer Service Excellence. Certified Home Remodelers earned Seaway's Executive Club award based upon their 2013 performance as an Authorized Dealer of Seaway replacement windows, doors and sunrooms.
4CDesignWorks, the integrative marketing and design division of 4CTechnologies, received two Hermes Creative Awards for its work in social marketing. The 2014 Hermes Creative Awards include a Platinum Award for the Social Madness Campaign in the social marketing category and a Gold Award for the Gallagher Performance Blog in the social media/blog category.
Working Order, a program of the Volunteers of America of Pennsylvania, was named National Business Incubation Association's 2014 Dinah Adkins Incubator of the Year in the general and special focus category.
MixByMe Technology, designed, developed and patented by Mouse Trap Design LLC, won the design and making category of the DATA2014 Design, Art and Technology Awards and Interactive Exhibition! sponsored by the Pittsburgh Technology Council. The company researches and develops packaging solutions.


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Ukulele Orchestra of Great Brooklyn Presents MISSIONARY IN MANHATTAN: A MORMON MUSICAL at UCB Tonight
The Ukulele Orchestra of Great Brooklyn, will present MISSIONARY IN MANHATTAN: A MORMON MUSICAL, a musical comedy about the fundamentals of big hair, blind faith, and just how plural a marriage can get, tonight, May 8th, 2014 at 8pm and Wednesday, May 21st at 8pm at Upright Citizens Brigade Theatre (307 W. 26th Street, between 8th and 9th Aves).
The Ukulele Orchestra of Great Brooklyn penned MISSIONARY IN MANHATTAN, the story of three polygamist-pop stars on the hunt for their brother husband in New York City. Charles Gregory Howard has gone missing and was last seen at The Cubby Hole with his vocal coach named Gerard. Ukuleles in tow, Gretchen, Fanny, and Bunny Catherine will turn pop songs into "God Songs," lament, and stop at nothing to track down the father of their seven children. Missionary in Manhattan: A Mormon Musical hysterically lampoons the institution of marriage as the sister wives learn to accept their newfound independence, and face the hard truth that "Grindr is not for bill pay, it's for boyfriends." The show runs at a super swift 30 minutes and will be performed on the main stage at Upright Citizens Brigade Theatre in Chelsea.
The cast of MISSIONARY IN MANHATTAN: A Mormon Musical includes Caitlin Davis, Kaleigh Malloy, and Elizabeth May. Scenic Design is by Flora Vassar. Direction by Chris Raddatz.
Performances of MISSIONARY IN MANHATTAN: A Mormon Musical are on 5/8 @ 8pm and 5/21 @ 8:00p. Tickets are $5 and available online at www.UCBTheatre.com.
The Ukulele Orchestra of Great Brooklyn provides a theatrical home for southerners and an invitation to those untouched by southern charm to "party til the cows come home." The founding members of UOGB challenge a generation criticized for its apathy, detachment, and reliance on technology by demanding audience engagement through outrageous comedy, musical celebrations, and honest storytelling.
About Author
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- CONSTELLATIONS' Jake Gyllenhaal and Ruth Wilson Set for THEATER TALK This Weekend


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I have problem in using VideoCapture calss for opening a MPEG video file. The code was compile properly. However, during the running time, it can not open the file and give me the following warning message:
warning: Error opening file (../../modules/highgui/src/cap_ffmpeg_impl.hpp:477)
I have this problem only when I build my code in debug mode. I relsease mode the code works correctly.
The code also works correctly in the c style using CvCapture and cvCaptureFromAVI (in both release and debug mode), however, I'd like to develop my code in more C++ style.
(I am using OpenCV 2.3 in Visual-studio (C++) 10)
Thank you for you helps


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Going off BCP (Yasmin)
Started by FrenchSoda , Dec 29 2012 05:11 PM
10 replies to this topic
Posted 29 December 2012 - 05:11 PM
Hi there,
I am going to stop taking the pill (Yasmin) after my next packet and commence charting (but not TTC until June). Silly question I'm sure, but should I finish the entire pack or just the active (yellow) pills? And should I expect my usual withdrawal bleed (assuming I stop after the last active pill)? If anyone here charted after coming off Yasmin, I'd be very grateful to hear about your experiences!
Thank you
Posted 29 December 2012 - 05:14 PM
Yes, you only need to finish the active pills, as the non-active week is just sugar to help us remember to take our pills You should expect your regular withdrawl bleed a few days after finishing.
I was on a different pill, but if you follow the link in my sig to my chart and scroll down to the bottom, you can see what my body did after going off the pill. My first month was anovulatory which is common but many women also get pregnant the first month after stopping the pill. Everyone is different
Good luck!
Posted 29 December 2012 - 10:00 PM
I just stopped when I finished the active pills, DD was conceived 2nd cycle Good luck!
Edited by Impatientmummy, 29 December 2012 - 10:01 PM.
Posted 30 December 2012 - 12:45 AM
Good plan to stop a while before starting to TTC. Some women experience a luteal phase deficiency for up to 6 months after stopping the pill. So know that this is normal to see confusing ovulation symptoms and temperature rises while your body gets back to normal. You may also experience a few long cycles and some cycles where you don't ovulate - again normal.
If you had cycle issues before going on the pill then they will probably resurface.
Posted 30 December 2012 - 01:17 AM
Yep, just finish the active tablets then expect your regular withdrawal bleed. I came off the same pill around 3 months before TTC so that I could chart for a bit and get to know my body's general pattern (as i had been on some sort of the pill for 9 years so really had no idea what my cycles would be like).
My first cycle was 5 weeks long (took 3 weeks to ovulate), all cycles after that were 4 weeks. I fell pregnant the first cycle trying. Hopefully you will experience similar!
Posted 30 December 2012 - 08:21 AM
Thanks so much for your replies, so helpful.
My cycles were like clockwork before going on the pill about 6 years ago, so fingers crossed they're not haywire for long when I come off! However my skin was atrocious pre-pill, so here's hoping that now that I'm no longer in the wake of puberty, it will behave!
A follow-up question: did you see your GP when going off the pill or for a pre-TTC appointment? I've been making sure I'm in tip-top health in the past couple of months (dental, skin cancer check, pap - and got the all-clear on a minor gyno issue) and was planning to go back to the doctor to let her know I was starting FAM (and prenatal vitamins, etc), but I'm wondering if it's necessary? I had thought maybe I should because I've had some vitamin deficiencies before, and my mum is rh-negative but I don't know my blood type, so perhaps the doctor would want me to get a blood test. Or she may just say come back when you're pregnant/been trying for 12 months?
Posted 30 December 2012 - 08:36 AM
A GP check up is a good idea. I think mine did a blood test tO check on vitamin deficiencies and thyroid function. I was on Yasmin for a few years and stopped taking it in March 2011. It took six cycles (seven months) to fall pregnant. I had a miscarriage and it took a further six months to get pregnant again, resulting in my son. I had a long first cycle off the pill, maybe 54 days. But I only used OPKs and did SMEP most of the time, as luck would have it, I became pregnant the first cycle I charted.
On the skin topic, I found my skin was pretty pimple prone after stopping Yasmin, even though it'd been fine when I started taking it. During pregnancy my skin cleared again and fingers crossed it is seems ok now.
Good luck!
Edited by little lion, 30 December 2012 - 08:37 AM.
Posted 30 December 2012 - 08:53 AM
Probably a good idea to go to the GP so that you can confirm your blood type, check your vitamin levels and immunity to rubella etc (also you might want to have a whooping cough booster if yours isn't up to date).
It is particularly useful to have your blood type on record incase you have any bleeding in your first trimester as I did (apparently it is quite common), as often a doctor will just do a urine test to confirm pregnancy and you may not have a full blood test until closer to 12 weeks when you are going through the NT testing (well that's what happened in my experience anyway, I'm sure doctors do things differently though).
Unfortunately I did get some pimples after coming off the pill but they cleared up during my pregnancy (they are back now that I have had DD and am breastfeeding so can only take the minipill, but I think to a lesser degree than before).
Good luck
Posted 30 December 2012 - 08:53 AM
I had a check up as I get low iron so wanted to be sure it was ok, I had been on pregnancy multi vitamins for 2 months prior as we planned to start ttc after we got married My cyles were clockwork pre pill & were 2 days shorter when I went off it.
Posted 30 December 2012 - 09:00 AM
As PP said go to Dr for some blood work. Get tested for Chicken pox immunity, rubella immunity, vitamin D levels and iron. Also get your blood type as if you are RH - like you mum you will usually need to get an Andi D shot if you have bleeding during the first trimester (very common..., I had bleeding in two out of three pregnancies). I am RH- so I needed the shots. I also had DH tested as if he was RH- too I would not have gotten the shots, but alas he was RH+.
Posted 31 December 2012 - 11:25 AM
Thanks so much for all your advice! I'll definitely pay the doc a visit.
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Desperate, out of petrol and low on food, a new mother lit a fire in the hope of attracting attention.
Boy found on swing died of hypothermia and dehydration, autopsy finds
The story was chilling and heartbreaking: a three-year-old boy was found dead in a Southern Maryland park, his mother pushing him on the swing.
Child's play and laughter help battle fatigue
Feeling fatigued? Uh-huh, thought as much. Join the queue.
Dad shares entertaining 'how to hold a baby' clip
For many new dads, their own child is the first baby they have ever held. So one dad has posted an instructive YouTube video titled "How to Hold a Baby".
The Australian baby with 100,000 Facebook fans
She may be only eight months old, but Egypt has already amassed more than 100,000 fans and received a letter from royalty - Hollywood royalty that is.
Public welcome outside church for Princess Charlotte's christening
Duke and Duchess of Cambridge have invited well-wishers to see Princess Charlotte outside church in Sandringham on day of her baptism.
Tongue tie: what you need to know
Tongue and lip tie can lead to many problems for babies - and their parents. Here are the signs of tongue tie and how it's treated.
My daughter is small but that doesn't matter
My daughter may be small, but it's my job as her parent to refocus back where it belongs - on who she is as a person
Wet wipes linked to rise in allergic reactions
The government has issued a health warning after a rise in allergic skin reactions has been linked to a preservative found in some wet wipes.
Gay couple in their 80s first to wed in Dallas after Supreme Court ruling
Love may have won, but it came with quite the wait.
William Tyrrell's family marks birthday with cake and renewed appeal
The family of missing boy William Tyrrell will mark his fourth birthday on Friday making a cake to share with friends and family as NSW police renewed their public appeal for information on his disappearance.
What all parents should know about safe babywearing
A picture of Ryan Reynolds always gets the girls talking, and a recently shared photo has done exactly that - but this time, it's for all the wrong reasons.
Baby's head shape reveals potentially fatal condition
Thinking her baby just had an unusually shaped head, a mother was shocked to discover it was instead linked to a dangerous condition.
'Help - my toddler hits me!'
My toddler has started hitting when he gets frustrated, is feeling ignored, or just thinks it might be fun.
Why IVF success rates may not be what you think
Transparency, accountability and responsibilityare essential measures to protect IVF vulnerable patients.
On the 10th anniversary of my son's death
This day marks a significant day. Today marks 10 years since I lost my son Kai.
Mother-in-law 'from hell' inspires survival guide
The happily ever after Nicola Milan had imagined wasn't to be – and she blames her mother-in-law.
Name your baby Quinoa, win a $10K gift card
Choosing a name for your little bundle of joy is always a major decision. It can be something traditional, trendy, creative … or inspired by the menu of your favourite chain restaurant.
Owning a pair of nail scissors does not make me a hairdresser
It's been a whole year since sleeping in until 10am. A whole year since having a peaceful shower.
The 83 children who were tragically let down in the last decade
Over a 10-year period, 83 children died from domestic violence abuse in NSW, with three quarters of the victims aged five years or under, the NSW Ombudsman has revealed.
Expert Q&A: Gross motor skill development in toddlers and preschoolers
Dr Katie Heathershaw answers questions about jumping, toe walking, riding a bike and being pigeon toed.
Is it reasonable to expect your partner to give up drinking in pregnancy?
From the moment that I fell pregnant with my son, I realised just how much my life had already started to change.
Stroke victim joins class action against makers of popular contraceptive pill
"I was terrified I would always be this way. The pill needs to come with a much higher warning."
Sexy time
Why you should get excited about scheduling sex
Unfortunately, the belief that sex should always be spontaneous is a myth. It just isn't.
When newborn photoshoots get messy
When it comes to newborn photoshoots, it is all about the timing.
Orphaned baby daughter Ayla wakes from coma
Former All Black Jerry Collins' critically injured orphaned daughter has awoken from her coma and is able to bottle-feed.
Dad takes miraculous catch while feeding baby
One American father has taken multitasking to a new level at a Cubs-Dodgers baseball game at Wrigley Field.
'Samuel is our firstborn, and he will never be forgotten'
Having lost their firstborn at one day old, the Carrolls were overjoyed to welcome their daughter Isobel into the world a year later.
Channel 10's Sarah Harris expecting first child
The Studio host Sarah Harris doesn't mind if her first baby is a boy or girl, but she does hope it is born with one thing in particular.
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27 funny ultrasound pictures
Ultrasounds give you a look at your growing baby ... and sometimes what appears to their womb-buddy, or your bub in an amusing position.
The top 6 misleading parenting terms
From 'morning sickness' to 'the terrible twos', there are many parenting terms that are misleading.
When 'good' nannies go bad
While most nannies take pride in their work, there can be some who have a hidden side.
Woman hospitalised for skinny jeans injury
Beware: skinny jeans might be bad for your health.
Gauze seeding: the bacteria-breeding birth trend
A number of women having caesarean deliveries are now taking steps to give their baby a better 'microbiome' start in life.
Jimmy Fallon writes new children's book for dads
Jimmy Fallon, host of NBC's The Tonight Show, recently wrote a children's book about every father's secret wish for their baby's first word to be "dada" - not "mama".
28 names for babies born in winter
Looking for some baby name inspiration for a bub born during the colder months? Here are 28 options from around the world to consider.
The horrible act that sparked a brawl at child's birthday party
The uncle of the seven-year-old girl at the centre of the brawl at child's birthday party in Sydney's west has described the events leading up to the alarming show of violence.
Babies 'benefit from iPads at a young age': study
More often than not, you'll read that screen time for children should be kept to a minimum - but some scientists are now challenging this way of thinking.
Do mums really just obsessively talk about their children?
Natalie Reilly describes three main types of conversations mothers have. And, surprise, they're not all about kids.
Why some dogs might attack babies or young kids
A baby's smell, the noises it makes and even its gaze can contribute to the potential for a dog attack.
Mum demands refund for 'beargina' christening cake
It was meant to be a tasteful cake to help celebrate a three-year-old's christening.
5 things no one warns you about after giving birth
How many times have you been warned about all the sleepless nights you have to 'look forward to' when you become a parent?
Police officer sang nursery rhyme as heartbreaking photo was taken
A police officer arrived at a devastating scene on Thursday: a car crash resulting in all passengers being thrown from the vehicle.
Don't worry, working mums: Just leave Dad in charge at home
Want to open the boardroom doors for women? Encourage - heck, praise - dads who stay home with their children.
Hilaria Baldwin shares post-baby selfie
Just two days after giving birth, actor Alec Balwin's wife posted a post-baby picture on social media.
'Help - my child won't ever do what I ask!'
Compliance is part of the parent-child relationship, but so is resistance. It's all natural.
Postnatal depression support gets $23 million boost in NSW
The Baird government will include $22.8 million in Tuesday's NSW budget to expand a program designed to help parents at risk of postnatal depression (PND).
'I'm just as tired, scared and stressed as you': stay-at-home dad's plea
I'm really lucky to have two great kids, but I found it really tough with so much being aimed at the mothers and not the fathers.
Get your FREE ticket to the Baby & Toddler Show
Get your free ticket to the Sydney Essential Baby & Toddler Show for September 25-27 - register online now.


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Developmental Divas Being Promoted To WWE Main Roster Soon?, Video: Survivor Series Ad Behind The Scenes
Originally written on cheap-heat | Last updated 11/14/12
- With WWE's Divas division centered on Eve, Kaitlyn and Layla week-in, week-out, many people within the company believe that more woman need to be featured imminently since there are zero new challengers left on either the face or heel side. F4WOnline.com states that a few developmental wrestlers could be promoted to the main roster to rectify this depth issue. - The Miz takes you behind the scenes of the making of his Survivor Series commercial. - According to WrestlingFigs.com, Sin Cara appears at HH Gregg 8921 US 31 S., Indianapolis, Indiana, this Saturday from 6:00 to 8:00 p.m. Call 317-888-2192 for more information.
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35 year old man | Burbank, CA, USA | Seeking women 23-31 within 50 miles of Los Angeles, CA
Active within 3 days
that rug really tied the room together.
The Short: - I am from Kansas ...queue 'Wizard of Oz' jokes and jeers ... yes I've been through countless tornadoes. - I also have a dog... not named Toto - I have a several brothers ... less than 6 more than 2 - I've lived in the same building as John Lennon - Pickles are a waste of a perfectly good cucumber - I am dutch... we live below sea level. - my grandfather looks like "David the Gnome" - I once saved a pigeon.. twice
Camping , Dining out , Gardening/Landscaping , Hobbies and crafts , Movies/Videos , Museums and art , Music and concerts , Exploring new areas , Playing cards , Playing sports , Travel/Sightseeing , Volunteering
Billiards / Pool, Bowling, Running, Swimming, Walking / Hiking, Weights / Machines, Yoga
I exercise 3-4 times per week.
Don't display my sign
I have Dogs
El Matador, Ventura, Elysian Park, and all things outdoors... especially near water. Cafe Stella, The Red Lion, Griffith Park, Little Dom's, The Alcove.
Music in nearly all its forms. I'm a bit of an audiophile. Independent Films/ Documentaries Hiking, Beach Volleyball, surfing, running, all things outdoors.
I listen to a lot of music. Mostly records, though I'm no elitist. Hearing an album, from start to finish, is an amazing experience. Hiking, drawing, bowling, surfing.
I have been reading a lot lately, mostly non-fiction. My current muse is "Cooked" by Michael Pollan.
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|6'0" (182cms)
|5'0" (152cms) to 5'10" (177cms)
|Body type:
|Athletic and toned
|About average
|No preference
|Dark brown
|Dark brown
|No Way
|No Way, Occasionally, Yes, but trying to quit
|Social Drinker
|Social Drinker
|I'll tell you later
|No preference
|I'll tell you later
|No preference
|Never Married
|Never Married
|Have kids:
|Wants kids:
|Definitely, Someday
|White / Caucasian
|White / Caucasian
I'm dutch.
|Spiritual but not religious
|Spiritual but not religious, Other, Christian / Other
|Graduate degree
|Graduate degree, PhD / Post Doctoral


text:
Lionsgate joins Jaman.com
Online movie download service Jaman.com has added another major studio's titles to its catalog, pacting with Lionsgate to offer select pics on demand.
Online movie download service Jaman.com has added another major studio's titles to its catalog, pacting with Lionsgate to offer select pics on demand.
Lionsgate has acquired the Duane Swierczynski novel "Severance Package" and has hired helmer Brett Simon ("Assassination of a High School President") to write the script with the author and direct.
Warner Bros. has set a release date for director Gavin O'Connor's long-gestating film "Pride and Glory." Meanwhile, O'Connor has set up his next pic, "Warrior," at Lionsgate.
Lionsgate posted stellar numbers in the fiscal first quarter as revenue shot up 50% and net profit swung to $7.1 million from a year-ago loss of $53.1 million.
Before selling the book, author and cartoonist Chris Millis has sold screenplay rights to his novel "God and California" to Lionsgate.
Lionsgate has tapped veteran exec Lisa Ellzey as exec VP of motion picture production as part of its efforts to broaden the banner's offerings.
John Stockwell has signed to direct supernatural thriller "Sanctuary" from Stephen Susco's script for Lionsgate and Sobini Films, which are co-financing.
Lionsgate film exec veep of acquisitions and co-productions Jason Constantine has been upped to prexy of the department.
Lionsgate has spirited away domestic and Canadian rights to the third "Transporter" movie starring Jason Statham ("Crank").
Lionsgate has acquired comedy spec "Stop Huntingdon Animal Cruelty" from Adam Sachs and set it up with Todd Garner's Broken Road Prods. and Energy Entertainment.
Lionsgate and Eros Intl., the leading distributor of Indian movies, have set up a joint venture spanning distribution and production in India and North America.


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It has not been lost on anyone watching the Union that Jack McInerney has scored just three goals in his 18 appearances since being part of the United States national team's Gold Cup roster last year.
It also has not been lost on those wondering when McInerney will fulfill his longstanding potential that the 21-year-old forward is nearing the end of his contract with the Union.
So if ever there was a time for the Union to get some value for McInerney, it's now.
That's why the Union dealt McInerney to the Montréal Impact on Friday for forward Andrew Wenger. The 23-year-old Lititz, Pa., native was the No. 1 pick in the 2012 SuperDraft, but has struggled to develop under the Impact's carousel of head coaches in recent years.


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ORCHARD PARK, N.Y., May 5 /PRNewswire-FirstCall/ -- MINRAD International, Inc. (Amex: BUF) today announced that it has entered into agreements to issue and sell an aggregate of $40.0 million of senior secured convertible notes with a group of institutional accredited investors in a private placement. The notes pay an 8% coupon, have a 3 year maturity and a conversion price of $2.65 a share. The aggregate net proceeds of the notes, after deducting the placement agents' fees and estimated offering expenses payable by MINRAD, are expected to be approximately $36.7 million. The Company plans to use the net proceeds of the financing to retire its obligations under its current $15.0 million senior notes with Laminar Direct Capital L.P. and for working capital and general corporate purposes. Lehman Brothers served as placement agent for MINRAD in the transaction.
The securities offered in the private placement were not registered under the Securities Act of 1933, as amended (the "Act") or any state securities laws, and may not be offered or sold in the United States absent registration, or an applicable exemption from registration, under the Act and applicable state securities laws. Pursuant to the purchase agreements with the investors, the Company will file a registration statement with the U.S. Securities and Exchange Commission covering the resale of the shares of common stock issuable upon the conversion of the notes, subject to certain terms and conditions.
In connection with the private placement, the Company is reaffirming
its previous guidance that revenue for the first quarter of 2008, which
ended March 31, 2008, will be in the range of $11.5 to $12.0 million.
Contact: Charles R. Trego, Jr., Chief Financial
|SOURCE MINRAD International, Inc.
Copyright©2008 PR Newswire.
All rights reserved


text:
By John Irish and Arshad Mohammed VIENNA (Reuters) - Iran and major powers gave themselves at least until Friday to negotiate an agreement on the Iranian nuclear program, but a source from one of the powers said on Tuesday they had to wrap up in the next 48 hours. "We are continuing to negotiate for the next couple of days," EU foreign policy chief Federica Mogherini said outside the hotel where the marathon talks between Iran, Britain, China, France, Germany, Russia and the United States are taking place. The spokeswoman for the U.S. delegation, Marie Harf, said the terms of an interim deal between Iran and the six would be extended through Friday to give negotiators a few more days to finish their work.
LOS ANGELES (AP) — Paula Deen didn't post a 2011 Halloween costume photo on her Twitter account showing her son Bobby in dark makeup as "I Love Lucy" character Ricky Ricardo, and the social media manager responsible for it was fired, Deen's spokesman said.


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1997 Chevrolet Venture Q&A
1997 Chevrolet Venture Question: Can heat/outside temprature have an effect on a car battery?
I can start my vehicle early in the morning before the temprature rises with no problem but as soon as the temprature gets hot it won't start. The cells are full. - silentone
Answer 1
As in drag racing when cars temperatures get really high they have problems starting. In a battery excessive heat causes corrosion and in turn hard starting = shortening life span of the battery. I forgot to add it can also be an intermittent problem with the main relay, starter, or fuel pump. - lude22r
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text:
Commodore Regent
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- Restaurant
- Free High-Speed Internet
- Room service
- Free parking
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TripAdvisor Reviews Commodore Regent Launceston
Travel Blogs from Launceston
... before nightfall of course, to suss out real estate. Friday and we wanted to walk to the gorge which was apparently a brisk 20 minute walk away. It didn't look too far away on the map but it was all uphill and a good workout for us all. We walked past a house one of our friend's lived in (hi Danielle) and took a photo of what it looks like now and texted it to her. We loved Cataract Gorge and can't believe something so beautiful is right in the heart of the ...
Lost Mt Arthur Found Georgetown and a flat tyre
... Bay.
As we drove out Allan ran over an old building foundation. "Ouch" said Sharon. However we didn't think much about it. We went into Launceston on our way to Evandale. Sharon needed new sneakers and there was a half price sale on. On our return from the shop there was a flat tyre.
Sharon is desperate to learn how to change the tyre in Vincent. She wants to manage on her own. ...
Caving and Gorging!
Had to leave our cabin today, and make our way to Launceston in the North East of Tassie. The low clouds and drizzle that have plagued since we came to Tasmania was back but gradually improved as we made our way east. There were a few attractions and view points along our route. The one that caught our attention was King Solomon's Caves. This a dry cave that was initially discovered about 1905, when two wallaby hunters saw one of their prey disappear down into it. They bought the ...
Deloraine Western Tiers country
... near Latrobe and bought some products made with cherries. Today we spent some time at the Trowunna Wildlife Park near Mole Creek and witnessed feeding time for some Tasmanian Devils (see attached short video). We are told they were named Devils because of the bloodcurdling noise they made when it was dark when they were fighting amongst themselves for food, and also their ears look like devils horns when a light is shone on ...
As Close As I Want To Get to Wildlife On This Trip
... aquaintance that Jake knew, and JB again.
Being JB's first visit to Australia and with half a day to kill, a visit to Trowunna Wildlife Park was planned. He saw Tassie devils, quolls, wedge-tailed eagles, Cape Barren geese, wombats, a boobook owl, a tawny frogmouth, cockatoos, kookaburras and he chased a kangaroo trying to get his perfect photograph.
After a bakery lunch it was a stop off ...


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In the first quarter of this year, with unseasonably warm dry weather tamping down wind flows in California, the amount of power generated by the state's 44 wind farms fell off by around 35% compared to the first quarter of 2014, according to data filed with the US Federal Energy Regulatory Commission and the Energy Information Administration compiled by Platts.
Archive for the 'renewable energy' Category
California's renewable power saga is just beginning
By Jeff Ryser and Eric Wieser | June 18, 2015 12:01 AM Comments (0)
How could a 'Brexit' affect the European energy market?
By Alex Froley | May 27, 2015 11:21 AM Comments (2)
The UK's premier business lobbying organization, the CBI, has called on the business world to "turn up the volume" in the debate about the country's relationship with Europe. A referendum is expected by 2017 to decide whether or not there should be a British exit (or "Brexit") from the European Union. But how could this impact the electricity and gas market?
Warming weather in California exposes vulnerabilities of wind power
By Jeff Ryser | May 14, 2015 12:01 AM Comments (4)
Is it the first signs of a serious problem for wind power generation?
Data shows a significant drop-off of wind power sales in California due to warm weather, which caused a drop in wind velocities. Is it possible that wind farms that so many believe will help mitigate the warming of a changing climate will instead be impaired by that very same warming?
More of Brazil's sugar scooped up for ethanol, but sweet spot elusive
By Patricia Manso | May 5, 2015 12:01 AM Comments (1)
When you're sitting on a shed load of sugarcane, it's nice to know you have options. For Brazil, as the powerhouse of global sugar production, that choice falls into two broad areas — make sugar, or make ethanol.
IHS CERAWeek 2015, Day 4: Power ballad ending
By News Desk | April 23, 2015 05:03 PM Comments (0)
Thursday is not the final day of IHS CERAWeek, but it is the final day focused on a particular commodity. It's also Platts' final day at CERAWeek this year. Thursday's focus on electric power allows for a wide variety of topics, though, from coal to actual power generation to natural gas and everything in between.
We had two editors roaming the sessions Thursday, one to focus on coal and one to focus on electric power. Some of what we heard was shared from @PlattsPower, although @PlattsCoal, @PlattsGas and @PlattsOil also got some fodder from various officials sharing their plans. Just as a good power ballad has to come to an end, our coverage of this year's CERAWeek is ending, and here are some thoughts from our editors about Thursday's events.
Guest post: Out of sight, out of mind? Vermont considers its renewables
By John Kingston | April 16, 2015 12:01 AM Comments (14)
John Kingston is president of The McGraw Hill Financial Global Institute and director of global market insights. He continues to observe energy markets after his many years with Platts.
There's another growing kerfuffle in Vermont, which we've written about before as it tries to balance a seemingly impossible array of choices as it moves forward with its energy future. It's a small state, but some of the conflicts there are sure to be duplicated in other parts of the US…and the world.
Revenge of the renewables: How wind and solar play in Germany and Texas
By Jeff Ryser | April 2, 2015 12:01 AM Comments (8)
Are big baseload power providers in Texas destined to suffer the same fate as their counterparts in Germany?
The question arises because Texas is once again undergoing a surge of wind generation installations at a time when wholesale power prices are already on the floor, and zero pricing due to existing wind generation is prevalent.
Guest post: A driving force behind the Low Carbon Fuel Standard sees credit prices rising
By John Kingston | March 31, 2015 08:01 AM Comments (4)
John Kingston is President of the McGraw Hill Financial Global Institute and Director of Global Market Insights. He continues to observe energy markets after his many years with Platts.
The price of Low Carbon Fuel Standard credits is going to rise. It's just a question of when.
UK election kicks off an uncertain period for energy investors
By Alex Froley | March 30, 2015 12:44 PM Comments (0)
The UK's general election kicked off Monday with the official closure of parliament, heralding 38 days of campaigning ahead of the May 7 vote. With clear policy differences between the parties and the polls too-close-to-call, energy investors could be set for an uncertain year ahead.
US EPA pleads for some RFS understanding
By Herman Wang | February 24, 2015 12:01 PM Comments (0)
Attendees of the National Ethanol Conference in suburban Dallas last week who were hoping to hear, at long last, what biofuels volumes the US Environmental Protection Agency would require in the overdue 2014 and 2015 Renewable Fuel Standards likely left disappointed.


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32 year old man | Mesa, AZ, USA | Seeking women 25-35 within 50 miles
New and trying to meet some interesting people.
I am a nice guy that like to shoot pool and have a good laugh. I like meeting new people and hear their story. I like all types of music, but I really enjoy rock.
Dining out , Movies/Videos , Museums and art , Exploring new areas , Playing cards , Video games , Watching sports
Billiards / Pool, Football, Weights / Machines
I exercise 3-4 times per week.
I have Dogs
I have two dogs a Dalmatian and a Chihuahua, they are just like my kids. The Dalmatian is my black and white shadow and always has to be near me.
Still new to the area and trying to find a good place I like to shoot pool at.
I like to go and shoot some pool and have a few drinks. I enjoy taking my dogs to the dog park and watching them run around and I sometimes run along with them. I like going to a rifle range and shooting my rifle, it is a great way to relax.
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|5'11" (180cms)
|3'0" (91cms) to 8'11" (271cms)
|Body type:
|Slender, About average, Curvy, Athletic and toned, Full-figured, A few extra pounds, Stocky
|Black, Blue, Brown, Grey, Green, Hazel
|Auburn / Red, Black, Light brown, Dark brown, Blonde, Salt and pepper, Silver, Dark blonde, Grey, Platinum, Bald
|Occasionally, Daily, Cigar aficionado, Yes, but trying to quit
|Social Drinker
|Never, Social Drinker, Regularly, Moderately
|Law enforcement / Security / Military
|No preference
|Less Than $25,000
|No preference
|Currently Separated
|No preference
|Have kids:
|Yes, and they sometimes live at home, No, Yes, and they live away from home, Yes, and they live at home
|Wants kids:
|Not sure
|Not sure
|Native American
|Asian, Black / African descent, East Indian, Latino / Hispanic, Middle Eastern, Native American, Pacific Islander, White / Caucasian, Other
|I'll tell you later
|Agnostic, Atheist, Buddhist / Taoist, Christian / Catholic, Christian / LDS, Christian / Protestant, Hindu, Jewish, Muslim / Islam, Spiritual but not religious, Other, Christian / Other
|High school
|High school, Some college, Associates degree, Bachelors degree


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Conceived and designed the experiments: ZX ZH. Performed the experiments: TKW. Analyzed the data: ZX XF TKW ZH. Contributed reagents/materials/analysis tools: ZX ZH. Wrote the paper: ZX XF TKW ZH.
Prevention of the initiation of biofilm formation is the most important step for combating biofilm-associated pathogens, as the ability of pathogens to resist antibiotics is enhanced 10 to 1000 times once biofilms are formed. Genes essential to bacterial growth in the planktonic state are potential targets to treat biofilm-associated pathogens. However, the biofilm formation capability of strains with mutations in these essential genes must be evaluated, since the pathogen might form a biofilm before it is eliminated. In order to address this issue, this work proposes a systems-level approach to quantifying the biofilm formation capability of mutants to determine target genes that are essential for bacterial metabolism in the planktonic state but do not induce biofilm formation in their mutants. The changes of fluxes through the reactions associated with the genes positively related to biofilm formation are used as soft sensors in the flux balance analysis to quantify the trend of biofilm formation upon the mutation of an essential gene. The essential genes whose mutants are predicted not to induce biofilm formation are regarded as gene targets. The proposed approach was applied to identify target genes to treat Pseudomonas aeruginosa infections. It is interesting to find that most essential gene mutants exhibit high potential to induce the biofilm formation while most non-essential gene mutants do not. Critically, we identified four essential genes, lysC, cysH, adk, and galU, that constitute gene targets to treat P. aeruginosa. They have been suggested by existing experimental data as potential drug targets for their crucial role in the survival or virulence of P. aeruginosa. It is also interesting to find that P. aeruginosa tends to survive the essential-gene mutation treatment by mainly enhancing fluxes through 8 metabolic reactions that regulate acetate metabolism, arginine metabolism, and glutamate metabolism.
Biofilms have been frequently associated with human diseases such as osteomyelitis [1], chronic wound infections [2], [3] and cystic fibrosis [4], as they facilitate the survival of pathogens in hostile environments. It is reported that nearly 65% of all nosocomial infections in the USA are associated with biofilms [5]. When exposed to stress, such as that imposed by antibiotic treatments or limited nutrients, pathogens adhere to each other to form biofilms for the purpose of survival [6]. The development of biofilms generally comprises the following four stages, i.e., the initial attachment of planktonic pathogens to a surface, the accumulation of biofilms through the production of extracellular polysaccharide substance (EPS) that interconnects and transiently immobilizes biofilm cells, the maturation of biofilm architecture, and the dispersal of single cells from the biofilm [7]. The first few stages play a key role in treating the biofilm-associated pathogens, as the ability of pathogens to resist antibiotics is significantly enhanced once they form biofilms [5], [7]. Therefore, significant effort in the biofilm research community has been devoted to the investigation of bacterial metabolism and signaling which are involved in the transition from planktonic growth to biofilm growth [8], [9].
Elucidating the mechanisms of biofilm formation is far from trivial: hundreds of highly interacted molecules such as metabolites, metabolic enzymes, and signaling proteins are involved in regulating this process. Most current research is focused on the experimental investigation of the impact of individual molecules, such as regulators, on biofilm formation [10], [11]. This is insufficient for characterizing the biofilm formation process, as a systems-level characterization of interactions between molecules involved in biofilm formation is required to fully understand biofilm formation mechanisms and thus manipulate the metabolism of microorganisms in biofilms. Genome-scale metabolic modeling has been commonly used for systemically studying microorganism metabolism, as evidenced by its wide application in identifying genes that are essential for the growth of Escherichia coli [12], Staphylococcus aureus [13], Mycobacterium tuberculosis [14], Acinetobacter baumannii [15], and Pseudomonas aeruginosa [16]. A recent study by Thiele and her coworkers [17] shows the first systems biology approach to identifying candidate drug targets for treating P. aeruginosa in biofilms. This approach mainly applies single/double gene inhibition simulations to determine the growth of P. aeruginosa in specific microenvironments that imitate microbial communities associated with biofilm formation. However, certain issues have not been addressed in this approach, including the quantification of the trend for mutants to form biofilms, and the identification of metabolic reactions that facilitate biofilm formation in mutants. This forms the motivation of this work, that is, to consider the trend of biofilm formation for mutants in the identification of drug-target genes.
Here, we propose an approach to identifying drug targets against a biofilm-forming pathogen, by identifying genes that satisfy two requirements: 1) these genes are crucial for the growth of the pathogen in the planktonic state, that is, the mutation of any of these genes can eliminate the planktonic pathogen; and 2) the inhibition of the function of these genes does not induce biofilm formation.
In particular, we first use the essential planktonic-growth genes presented in Oberhardt et al., 2008 [16] as the initial set of genes satisfying the first requirement, and further, from the initial set, identify genes also satisfying the second requirement. We perform the search by pinpointing a set of biofilm associated reactions from the genes that are reported to be positively related to P. aeruginosa biofilm formation in Müsken et al., 2010 [18], and using the flux changes through these reactions as soft-sensors to quantify the impact of the mutation of each gene from the initial set on biofilm formation. The rationale for the use of biofilm-associated reactions is that a large enhancement of fluxes (i.e., activity levels) through these reactions reflects a large potential for a mutant to form biofilms. Specifically, to obtain the flux changes through biofilm-associated reactions, we simulate the metabolism of a gene mutant via flux balance analysis (FBA) [19], and sample the biofilm-associated reaction fluxes via the artificially-center-hit-and-run method (ACHR), an efficient sampling approach for a linearly constrained space [20]. In addition, metabolic reactions whose fluxes significantly increase in most mutants can be determined and regarded as reactions that facilitate biofilm formation. P. aeruginosa is chosen as the reference pathogen in this work, because P. aeruginosa is one of the leading causes of nosocomial infections in hospitalized patients and P. aeruginosa is resistant to a wide array of antibiotics by forming biofilms during chronic infections [21], [22].
The key innovation of the proposed approach is to take the biofilm formation of a single mutant into account when identifying gene targets to treat biofilm-associated pathogens. While the detail of the proposed approach is given in the Material and Methods section, an example is shown in Figure 1 to illustrate the steps for the identification of drug targets that impair the growth of P. aeruginosa without inducing biofilm formation. The genes positively associated with biofilm formation (obtained from Müsken et al., 2010 [18] for P. aeruginosa in LB medium) are first overlaid with the P. aeruginosa metabolic network to identify reactions associated with biofilm formation (Figure 1, Step 1). The metabolic model presented in Oberhardt et al., 2008 [16], which contains 1056 genes and 883 metabolic reactions, is used in this work, as it is the most comprehensive metabolic model for P. aeruginosa and it has been used to correctly predict catabolism of various substrates such as amino acids and common sugars via flux balance analysis. Further, we quantitatively evaluated the impact of the mutation of each essential planktonic-growth gene (obtained from Oberhardt et al., 2008 [16] for P. aeruginosa in LB medium, including the PA1756 gene) on biofilm associated reactions, by calculating relative activity changes of these reactions via Steps 2 and 3. The obtained relative activity change profile quantitatively suggests the capability of a mutant to form biofilms, that is, large values for the changes imply a potential induction of the mutant's biofilm formation. In Step 4, the similarity in the shape and magnitude of relative activity profiles is used to categorize the essential planktonic-growth genes [16] into different clusters. The clusters of essential planktonic-growth genes whose mutants have low potential to induce biofilm formation are identified as drug-target genes (such as PA1756), because the mutation of these genes can eliminate planktonic pathogens. Metabolic reactions whose activity levels significantly increase in most single mutants are identified in Step 5. These reactions illustrate how P. aeruginosa adjusts its metabolism to form biofilms upon the mutation of genes essential for planktonic growth
A set of biofilm-associated metabolic reactions are obtained via Step 1 in Figure 1. The experimental data of genetic determinants of P. aeruginosa biofilm have been presented in Müsken et al., 2010 [18]. 394 genes are reported to be positively associated with biofilm formation, and 64 of them are involved in the metabolic network. None of these 64 genes are essential for bacterial growth in the planktonic state. Flux balance analysis showed that inhibition of 37 of these 64 genes doesn't affect bacterial growth rate. These genes are thus not directly involved in the bacterial biomass synthesis. Fluxes of reactions associated with these 37 genes are the ideal virtual sensors that monitor the trend of P. aeruginosa to form biofilms, as these genes are only associated with biofilm formation. The 39 reactions associated with these 37 genes are considered as biofilm-associated reactions (see Table 1).
It can be seen from Table 1 that the biofilm-associated reactions are mainly involved in the consumption of nitrite, the regulation of acetate, tricarboxylic acid (TCA) circle, the generation of carbon dioxide and ammonia, iron metabolism, the regulation of hydrogen peroxide, arginine metabolism, the production of glutamate, pyrimidine metabolism, and oxidative phosphorylation. While these reactions were identified by overlaying the 37 genes that are reported to be associated with biofilm formation in Müsken et al., 2010 [18] onto the metabolic network of P. aeruginosa, their important role in biofilm formation is further confirmed by the following independent evidence:
Given the above biofilm-associated reactions, we obtained a relative activity change profile of these reactions for each single mutant of the 136 essential planktonic-growth genes obtained from Oberhardt et al., 2008 [16] (via Steps 2 and 3), and, subsequently, based upon the similarity in relative activity change profiles for different single mutants, went through Step 4 to hierarchically categorize the essential planktonic-growth genes into six different clusters (as shown in Figure 2). Clusters 1 and 2 are located in one branch, while the other four clusters are associated with the other branch. The hierarchical clustering algorithm allows us to further separate genes in each cluster into different groups according to the similarity in their relative activity change profiles. The genes associated with each group shown in Figure 2 are listed in Table 2.
The profiles of relative activity change for the mutants of the representative groups for different clusters are shown in Figure 3. Figure 3 (A) and (B) show the profiles for the mutants of the PA0945 (from Group 1, Cluster 1) and PA5038 genes (from Group 25, Cluster 1), respectively. The profiles of these two mutants are used to represent the whole spectrum of Cluster 1, because, although they are from the two groups (Groups 1 and 25) with the lowest similarity in the cluster, their profiles of relative activity change in Figure 3 (A) and (B) are similar, suggesting that the single mutations of genes in Cluster 1 have similar impacts on biofilm-associated reactions and thus potentially on biofilm formation. Figure 3 (C) through (G) show the relative activity change profiles of representative groups from Clusters 2 through 6, respectively. Since there is only one group (actually one gene per group) associated with each of these clusters, these groups are regarded as the representatives of the corresponding clusters (as shown in Figure 2).
Single mutants of genes from different clusters have different potentials to induce biofilm formation. As shown in Figure 3 (A) and (B), the activity levels of certain biofilm-associated reactions have significant increases upon the mutation of a gene from Cluster 1, suggesting that the gene mutants from Cluster 1 might form biofilms and in turn prevent the elimination of P. aeruginosa. The comparison of Figure 3 (C) to Figure 3 (A) and (B) shows that mutants from Cluster 2 might still form biofilms but to a lesser extent. In particular, the mutant from Cluster 2 (PA4031) and those from Cluster 1 (PA0945 and PA5038) have similar relative activity changes in the biofilm-associated reactions Rxn#1 through Rxn#32, but the mutant from Cluster 2 has much smaller changes in Rxn#33 through Rxn#39 than those from Cluster 1. Interestingly, as shown in Table 2, there are totally 132 essential genes associated with Cluster 1 and Cluster 2, while only the remaining four are from Clusters 3 through 6, suggesting that the mutation of most essential planktonic-growth genes might induce the formation of biofilms. Therefore, not every essential planktonic-growth gene is a good target to treat biofilm-associated pathogens.
The mutation of any gene from Clusters 3 through 6 might not induce biofilm formation, as approximately 96% of the biofilm-associated reactions for these mutants are of small relative activity changes (e.g. most less than 0.5). In addition, the relative activity changes of certain biofilm-associated reactions are reversed and have negative values. Even though the amplitudes of these negative relative fold changes are minor (e.g., most less than 0.5), the metabolic activities are not distributed in the direction for promoting biofilm formation for the mutants from Clusters 3 through 6. Therefore, the four genes in these clusters, i.e., PA0904 (lysC), PA1756 (cysH), PA3686 (adk), and PA2023(galU), are regarded potential gene targets to treat P. aeruginosa, because they are essential to planktonic P. aeruginosa and their mutants might not induce biofilm formation.
Experimental data were collected from the literature to validate the aforementioned prediction results, which shows that the mutants from Clusters 1 and 2 might induce biofilm formation while the mutants from Clusters 3 through 6 might not. The essential genes contained in Clusters 1 and 2 are mainly involved in vitamin and cofactor synthesis, amino acid catabolism, cell wall synthesis, central metabolism, the membrane transport system, nucleotide synthesis, nucleotide salvage, and lipid synthesis. The involvement of these genes in the aforementioned biological systems is listed in the Table S1. The mutants of some genes in Cluster 1 have been reported to form biofilms. For example, the inhibitor of the enzyme encoded by the glmU gene (Group 4) is able to enhance the formation of biofilms of P. aeruginosa PAO1 [36]. Another example is given that the pgsA null mutant (Group 4) of E. coli activates the Rcs signal transduction pathway that is crucial for E. coli biofilm formation [37]. In addition, the down-regulation of the following six essential genes, i.e., pyrH (Group 6), tktA (Group 22), tpiA (Group 19), rpiA (Group 24), dapD (Group 4), and rmlA (Group 4), is reported as the driving force for Streptococcus biofilm formation [38].
As seen from Figure 3, the activity levels of all the biofilm-associated reactions are small for the genes from Clusters 3 through 6, i.e., PA0904 (lysC), PA1756 (cysH), PA2023(galU), and PA3686 (adk). Hence, they are regarded as ideal gene targets to treat P. aeruginosa infections because the inactivation of any of them can kill the planktonic pathogen cells but cannot promote the switch from planktonic growth to biofilm formation. The following experimental evidence further confirms these four genes are good targets to treat biofilm-associated P. aeruginosa:
An interesting finding by inspecting Figure 3 (A) through (C) is that certain biofilm-associated reactions have significantly increased activity levels (e.g., more than three-fold) upon the single mutations of all the three representative genes from Cluster 1 and Cluster 2, i.e., PA0945 (purM), PA5038 (aroB), and PA4031 (ppa). This implies that, although these metabolic reactions are all associated with biofilm formation, certain parts of them are more likely to be utilized by P. aeruginosa upon the stress such as that imposed by the mutation of essential planktonic growth genes.
Therefore, we analyzed the relative activity changes of each biofilm-associated reaction across all single mutants, and categorized the 39 biofilm-associated reactions into two types: 1) the reactions with minor increase of activity levels for most essential mutants and 2) those with significant activity increase. Figure 4 (A) and (B) show the typical relative activity changes of two biofilm-associated reactions: a minor-increase reaction (Reaction Rxn# 1) and a "significant-increase" reaction (Reaction Rxn# 4). The relative activity changes of other biofilm-associated reactions are similar to either the one for Rxn# 1 or that for Rxn# 4 (data not shown). Reactions associated with each of the two types of reactions are listed in Table 3.
The "significant-increase" type of reactions have significant increases in activity levels for the mutants of most essential genes, and might reveal the underlying mechanisms for biofilm formation when P. aeruginosa is treated by the antimicrobial agents that attack the essential genes. It can be seen from Table 3 that these reactions are mainly involved in acetate metabolism (mainly via Rxn# 4 and 35), arginine metabolism (mainly via Rxn#27 and 34), glutamate metabolism (mainly via Rxn# 5 and 36), the regulation of hydrogen peroxide (mainly via Rxn# 22), and the phosphate transport (mainly via Reaction Rxn #28). These findings are supported by existing experimental data listed as follows.
While all the aforementioned experiments focus on the investigation of the impact of individual metabolic modules on biofilm formation, this work represents the first mathematical modeling approach for the systemic identification of the underlying metabolic mechanisms that facilitate biofilm formation of single-mutants.
The formation of biofilms facilitates the survival of disease-causing pathogens in hostile environmental conditions. Therefore, preventing the pathogen's transition from the planktonic state to the biofilm growth mode is one of the most important steps to treat biofilm-associated pathogens. Since the metabolism of pathogens is determined by the interaction of hundreds of metabolic reactions, genes, and enzymes, systems biology approaches can facilitate gene target identification for preventing the planktonic to biofilms transition. In this work, a systems-level analysis approach was presented to answer the following question that still remains unanswered, that is, how to mathematically quantify the capability of a pathogen to form biofilms upon the mutation of a specific gene. The fluxes through the reactions associated with an essential planktonic-growth gene are limited to 10% of their nominal values in flux balance analysis to mimic the mutation of the gene in this work. Although the approach used here is a partial shutdown instead of a complete gene-knockout mutation, it better reflects the response of pathogens to the treatment of antimicrobial agents, which generally cannot immediately eliminate the biological functions of the target genes. The mutant might form biofilm before antimicrobial agents completely eliminate the bacterial metabolism. In addition, the pathogen might be treated by a sub-inhibition dose, which can be mimicked by setting the allowable fluxes to 10% of their nominal values. The terminology "mutant" or "mutation" is still used in this work, although an in silico partial shutdown mutation is performed to identify gene targets from those essential planktonic-growth genes of P. aeruginosa.
Based upon the results in this work, it is interesting to hypothesize that the mutations of essential genes are more likely to induce P. aeruginosa biofilm than those of non-essential ones. For essential genes, the mutations of 132 out of the 136 essential planktonic-growth genes were predicted to induce biofilm formation, and the down-regulation of 8 of them, i.e., pyrH, tktA, tpiA, rpiA, dapD, and rmlA [38], glmU [36], and pgsA [37], has been experimentally proven to be positively correlated with biofilm formation. In contrast, we find that only two out of 920 non-essential mutants might induce biofilm formation, when applying the proposed approach to evaluate the biofilm formation capability of mutants of the nonessential genes in the P. aeruginosa model reported by Oberhardt et al., 2008 [16]. For the 920 non-essential genes, we have followed the experiment approaches presented in Ueda et al., 2009 [34], to carry out comprehensive screening experiment for altered biofilm mutants from the PA14 non-redundant transposition mutant library (Liberati et al., 2006, [49]). The library contains 835 of the 920 non-essential genes, and of these 835 mutants, 823 have been verified not to induce biofilm formation (data can be provided upon request). While we predicted a lower number of mutants that form biofilms (i.e., two by prediction versus 12 by experiment), this is probably because we use a conservative approach in mimicking partial shutdown mutation, in which a reaction that is catalyzed by multiple enzymes is not inhibited for single mutants, but on the whole the results were verified for the vast majority of the bacteria with mutations in non-essential genes. The potential reason for explaining the hypothesized strong biofilm formation capability of most essential mutants is that the mutation of an essential planktonic-growth gene might cause limited nutrient uptakes, which strongly enhance biofilm formation [6], and make the pathogen feel the stress, which is reported as one of the major driving forces for biofilm formation [50]. On the contrary, when a non-essential gene is inhibited, the pathogen does not feel very much stress, because non-essential genes are not crucial for the biomass synthesis. This hypothesis as well as the findings of the biofilm formation phenotypes of single mutants forms the foundation for the further experimental investigation.
This work was mainly focused on investigating the biofilm formation capability of single mutants, as the single gene inhibition is easier to implement than the multiple gene inhibition. While it is possible to apply the proposed approach to multiple-mutants, the ACHR sampling approach needs to be upgraded to improve the computational efficiency. It takes approximately 15 minutes to obtain 20,000 samples of fluxes of the biofilm-associated reactions upon the mutation of a single gene for a desktop computer with Intel Core i5 2.5 GHz CPU and 8 GB RAM. Since 920 nonessential genes have been reported in Oberhardt et al., 2008 [16], it is very time-consuming to study the biofilm formation ability of all possible multiple-mutants. Nevertheless, the study of the impact of the multiple-gene mutation on biofilm formation is an interesting topic for the future research.
To the best knowledge of the authors, this work represents the first systems-level approach to incorporate the quantification of biofilm formation capability in evaluation of genes as the targets to treat biofilm-associated pathogens. Four essential planktonic-growth genes, i.e., PA0904 (lysC), PA1756 (cysH), PA3686 (adk), and PA2023(galU), were identified as the potential gene targets to treat P. aeruginosa, as the mutation of any of these genes can eliminate the pathogen without inducing biofilm formation. This finding is implied by existing experimental data. Based upon the relative activity change of biofilm-associated reactions over all the single mutants, it is interesting to find that the fluxes of approximately 8 biofilm-associated reactions significantly increase for most single essential mutants. They are mainly associated with acetate metabolism, arginine metabolism, and glutamate metabolism. All these findings can be used to generate hypotheses for experiment design. In addition, the proposed approach can be applied to identify gene targets for treating any other biofilm-associated pathogen if the genes positively associated with biofilm formation have been identified and the metabolic model has been developed for the pathogen.
In this section, the systems-level approach whose outline is shown in the illustrative example (Figure 1) is described. This approach is used to quantify the biofilm formation capability of single essential mutants, cluster essential genes according to the biofilm formation capacity of their mutants, and systematically identify the metabolic reactions whose activity levels significantly increase for most single essential mutants. The detail of the proposed approach is shown step by step as follows.
The distribution of the essential genes from Cluster 1 of Figure 2 in biological subsystems.
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The Top 10 In Action Sports
01Brick By Brick: Camping Lego Style
02Booze and Bikes: 14 Tours That Let Combine Two of the Best Things in the World
03Who Needs Hollywood? These Incredible Videos Were All Filmed on the Simplest Cameras
04Sam Pilgrim vs. The World: The Champion Aiming To Reclaim His Crown In A New Era Of Biking
05Eccentric Adventures Episode 2: Britain's Most Brutal Illegal Event is All About Cheese
06A Journey Into Darkness: We Went Mountain Biking in the Abandoned Mines of Slovenia
07After the Apocalypse: Inside The World's Most Breathtaking Ghost Towns
08Debate: Let's Stop Hating On Parents Who Want To Raise Adventurous Kids
09Guns, Roses, And Eating Insects: Adventure Cycling in Vietnam
10From Intense Trails to Intensive Care: Dicing with Death In a Mountain Biking Paradise
Cutting Room Floor: Max Anstie
Way back in February we took a trip over to the good ole US of A to visit British GP contender Max Anstie at his home in Hemet, CA. Somehow the footage from that day was lost until now. This video was shot a week or so before Anstie left the states to make his eagerly anticipated return to racing in Europe with the CLS Pro Circuit Kawasaki squad. Anstie had a mixed year in his first season of GP racing but he proved many times he had the speed to run with the big boys. This video is proof of Max's determination and dedication to the sport we all love so much. We hope to see max back in Europe next year but at the moment it is unclear were Max will end up.


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July 31 - August 2, 2015
Three days. Five unique components from which to choose. Twenty-five women-owned farms. SOIL SISTERS celebrates Wisconsin's family farms and rural life in and around the farming communities of Monroe and Brodhead, Wisconsin, over the first weekend of August, 2015.
Starting on July 31 and running through August 2, 2015, SOIL SISTERS offers an immersive farming and culinary experience, led by women - the "soil sisters" -- committed to healthy, fresh products or farm-made, artisanal food products and other items. Some of these soil sisters are assisted by their husbands (aka "brothers") and/or their family. From heirloom tomatoes to emus, sheep to solar energy, bed & breakfasts to beef, the farmers and artisan food producers share a unique diversity of farm experiences showcasing the summer's bounty.
Choose from a variety of activities including Dinner on the Farm, a Taste of Place culinary event, plentiful on-farm "Green Acres" fun and educational workshops, plus area restaurants featuring "locavore" specials throughout the weekend. Depending on the Green Acres workshop, you can milk a goat, spin some fiber, go on a hayride, learn how to ferment, or build a birdhouse, among many other hands-on activities. Create your own farm and culinary adventure itinerary.
So, wear your sun hat, pull on your farm boots or overalls, and kick back for a relaxing time on one, or all, of the farms. And come hungry for some farm-to-table feasts.
Key Links:
Website: www.soilsisterswi.org
Facebook: https://www.facebook.com/SoilSisters
Workshop and culinary event registrations NOW OPEN on Brown Paper Tickets: http://www.brownpapertickets.com/producer/985898
Google+: https://plus.google.com/111771840588415123982
Sponsored by Wisconsin Farmers Union Foundation
July 2, 2015
• Solar Power Hour, Thursday, July 2, 6:30 p.m., Michael Fields Agricultural Institute, W2493 County Road ES, East Troy.In partnership with Michael Fields Agricultural Institute.
For more information contact Sarah Lloyd, Special Projects Coordinator, 608 844 3758, email@example.com.
July 9, 2015
• Solar Power Hour, Thursday, July 9, 6:30pmHungry Turtle Farm and Learning Center, 110 Keller Ave. N., Amery.In partnership with the Hungry Turtle Farm and Learning Center
For more information contact Sarah Lloyd, Special Projects Coordinator, 608 844 3758, firstname.lastname@example.com.
December 3-4, 2015
The Plaza ~ Eau Claire, WI
The Midwest CSA community is invited to come together to network and learn from each other to strengthen CSA farms and the local food system. The Wisconsin Farmers Union will again convene this gathering, featuring practical presentations for the CSA movement regarding production, packaging and logistics, business management, community building, and more.
To see materials from our 2013 meeting click here and scroll down a little under the Initiatives heading.
Wisconsin Farmers Union
117 West Spring StreetChippewa Falls, WI 54729
715-723-5561Fax: 715-723-7011
Country Hedging Market Bids
Farmer's Share of Retail Food
Wisconsin State Farmer
WI Department of Agriculture, Trade, and Consumer Protection
National Farmers Union
Copyright © 2015 Wisconsin Farmers Union


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Mauricio is a professional in every sense of the word. When purchasing my current home not olny was he able to find the property that fit exactly what we were looking for. He was intrumental in negotiating with the previous owner and lowering the price significantly. In my estimates maurico saved us aproximately 20% from what our property was listed for.
Mauricio Umansky
3 Recent Sales (last 12 months)
CEO/ Co-founder/ Realtor
- Specialties:
- Buyer's Agent,
- Listing Agent,
- Relocation
Ratings & Reviews
Bought a Single Family home in 2003 for approximately $625K in Sherman Oaks, Sherman Oaks, CA.
Sold a home in 2010 in Hollywood Hills, Los Angeles, CA 90069.
Have done numerous transactions with this Agent on both sides of the deal and he is not just extremely knowledgeable but understands the process and how to make a deal happen. He shines best in difficult situations, especially between Buyer and Seller.
Sold a home in 2009.
I have known Mauricio for many years and have had the pleausre of dealing with him on a few transactions which at times he was either represeting me or the seller. He has always conducted himself professionally and has always been very clear to help manage expectations. I will gladly refer him and his company to anyone in need of an agent with the depth and experience that he embodies.


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Back to Pandora: Why Has 'Avatar' Been Forgotten Just Five Years After Its Release?Matt Singer December 30, 2014 @ 2:36 PM
James Cameron's 'Avatar' opened on December 18, 2009, five years ago this month. In a theatrical release that would stretch on for 34 weeks, Cameron's motion-captured 3D spectacle grossed $749 million in the U.S. and an additional $2 billion overseas. Box-office-wise, it is the biggest movie in history by an absurd margin; it tops its closest competition, Cameron's own 'Titanic,' by some $600 million. That's more than 'The Dark Knight' made in its entire domestic theatrical run.


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Huron River Cleanup
Cleanups are held every spring and autumn.
Saturday, May 16, 2015 – Ypsilanti
Cleanup from 9am to Noon. Meeting location will be sent to registrants (near Depot Town). After party will run from Noon to 2pm at Riverside Park. Register by emailing Jason at email@example.com.
Saturday, August 15, 2015 – near Flat Rock and Milford
Save the date! Details coming online soon.
Please set aside these days to help the Huron, collect trash, meet and greet, trade stories and information, and generally enjoy a day on the river.
Pick a section of the river. Start whenever you like, work in a team or alone, with a canoe/boat or on foot (waders generally preferred) and some heavy duty trash bags and gloves.
If you need watercraft. We are sometimes able to provide a limited number of canoes. For those needing a watercraft you must email Jason in advance at: email@example.com. Watercraft pickup locations TBD between 8 and 10am.
HELPFUL HINTS: Cleanup volunteers need to provide their own gear to collect the river trash. Although HRWC has some trash bags and gloves and grabbers they are limited. It helps if you have your own gear.
Items to bring along:
- Waders, aqua shoes, sunscreen, canoe, kayak, life jackets, or rowboat
- Gloves to protect your hands
- Long handle or stick with a hook on it or a nail for spearing trash or retrieving it from the bottom
- HEAVY DUTY trash bags (stronger than the average household use bags)
- HRWC will have some trash bags but if you have your own please bring them
- Polarized sunglasses for spotting trash on the bottom
After party: We often have a potluck/BBQ after the cleanups, please join us!.)
Sponsored by: HRWC, DEQ, KEEN, REI, Ann Arbor Trout Unlimited, Huron River Fly Fishing Club, Schultz Outfitters, Huron-Clinton Metropolitan Authority, Skips Canoe Livery.