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BARACK OBAMA BIRTH CERTIFICATE: Suit contesting Obama's citizen...
There are 192029 comments on the Chicago Tribune story from Jan 8, 2009, titled BARACK OBAMA BIRTH CERTIFICATE: Suit contesting Obama's citizen.... In it, Chicago Tribune reports that:The U.S. Supreme Court will consider Friday whether to take up a lawsuit challenging President-elect Barack Obama 's U.S. citizenship, a continuation of a New Jersey case embraced by some opponents of Obama's ...
Join the discussion below, or Read more at Chicago Tribune.
#120468 Oct 25, 2012
It's a coastal city sitting on the Mediterranean sea. We have aircraft carriers, with air planes, helicopters, submarines, and other ships.
Freedom of movement.
Ambassador = dead
3 others = dead
Al Qeada = did it
obama = went to Vegas
drones = for all to see REAL time
coverup = bigger then Watergate
movie = to blame, THEY say
LIES from 0 admin
North York, Canada
#120470 Oct 25, 2012
Was he AlQaeda? If so, who cares what nationality?
#120471 Oct 25, 2012
Please indicate where in the Constitution a dual-citizenship status is allowed.
#120472 Oct 25, 2012
Anti-Israel, Pro-Hamas Muslim Leader Is
*** US Delegate ***[WHY]
to Warsaw Human Rights Conference
His most infamous statement was during a radio interview on September 11, 2001, accusing Israel of the attack on the WTC.
North York, Canada
#120473 Oct 25, 2012
1. Please indicate where in the Constitution a dual-citizenship status is NOT allowed.
2. De facto. Ever heard of it? Thousands of Americans and Canadians have dual citizenship. They have it, they keep it, they have two passports. Why are U.S. authorities allowing this to continue?
#120474 Oct 25, 2012
I hereby declare, on oath, that I absolutely and entirely renounce and abjure all allegiance and fidelity to any foreign prince, potentate, state, or sovereignty of whom or which I have heretofore been a subject or citizen; that I will support and defend the Constitution and laws of the United States of America against all enemies, foreign and domestic; that I will bear true faith and allegiance to the same; that I will bear arms on behalf of the United States when required by the law; that I will perform :noncombatant service in the Armed Forces of the United States when required by the law; that I will perform work of national importance under civilian direction when required by the law; and that I take this obligation freely without any mental reservation or purpose of evasion; so help me God.
I would say, our government is rotting from the inside-out. Just look, our president is an illegal alien.
#120475 Oct 25, 2012
Fascinating. More proof that Dale's fantasy includes his own personal crayola constitution
#120476 Oct 25, 2012
Re: "I would say, our government is rotting from the inside-out. Just look, our president is an illegal alien. "
Actually, Obama was born in Hawaii as his birth certificate, and the confirmation of the officials in Hawaii of both parties and the birth notices in the Hawaii newspapers placed by the DOH of Hawaii in 1961 all show.
#120477 Oct 25, 2012
which has been proven to be BOGUS.
"Facts trump speculation"
Since: Dec 08
#120478 Oct 25, 2012
Pipe dream.
#120479 Oct 25, 2012
Answer: Only birther "experts"---who have not proved that they are really experts, and who certainly have not proved that they are fair and impartial say that there is anything wrong with the image of Obama's birth certificate.
The birther "experts" include Paul Irey, who has repeatedly claimed that Obama did not attend Columbia College, despite the fact that Columbia University has stated that Obama did attend (and that he graduated). And they include Doug Vogt, who claims to have found the original altar of Abraham. It is such "experts" as these that the birthers rely on for their case.
There are many real experts who say that there is nothing wrong with Obama's birth certificate, including John Woodman, who happens to be a member of the Tea Party and who dislikes Obama and Obama's policies, but shows that the birther "experts" are wrong. They also include Ivan Zatkovich, who was hired by WND to examine the image of Obama's birth certificate, and when he submitted his report that there was nothing wrong with Obama's birth certificate, WND simply did not publish it.
As for Sheriff Joe and his posse:
It is clear that rational conservatives do not believe the "birth certificate was forged" claims. Ann Coulter, Glenn Beck and the National Review certainly do not.
And the conservative secretary of state of Arizona asked Hawaii to confirm that Obama was born there and to confirm specified facts on Obama's birth certificate. Hawaii did, stating that it compared the image of Obama's birth certificate with the file copy (which is an additional confirmation that there was a file copy BTW) and that Obama was born there and that the facts match. The secretary of state of Arizona accepted this confirmation and ruled that Obama will be on the ballot in Arizona in November. This indicates that he does not believe the claims that the birth certificate is forged (including those of Sheriff Joe) and that he does believe the officials in Hawaii.
How do we know that there is a birth certificate for Obama in the Hawaii files?
The first reason is the short form birth certificate. That was created by a clerk copying the information off of the original document in the files. If there were no document, there could be no short form birth certificate. Hawaii has confirmed that it sent a short form birth certificate (COLB) to Obama.
The second reason for knowing that there is a birth certificate in the files is that Hawaii said that there is, several times, under both Republican and Democrat governors.
The third reason was that the current director of health of Hawaii stated in a letter that she had seen the long form birth certificate being created by photocopying onto security paper the original document that was in the files.
The fourth reason is that the existence of Obama's birth certificate in 1961 is proven by the birth notices sent to the newspapers of Hawaii in 1961 by the DOH of Hawaii. Only the DOH could send those notices to the Health Bureau Statistics section of the papers, and the DOH sent only sent those notices for children whom it had issued birth certificates for.
Would you like me to post the citations of the confirmations by the officials in Hawaii?
#120481 Oct 25, 2012
If anyone were to have pipe dreams it would certainly be the Meth Chef. Methhead.
#120482 Oct 25, 2012
Obama White House Knew Al-Qaeda Ally Hit Benghazi
Two hours after last months deadly assault on the U.S. mission in Benghazi, Libya, the Obama White House knew the operation had been orchestrated by Muslim terrorists with ties to al-Qaeda, new evidence demonstrates.
But that grim information failed to deter President Obama, who has consistently been more interested in golfing than in attending security briefings, from hopping onto Air Force One on the eleventh anniversary of 9/11 and jetting off to Las Vegas for a campaign fundraiser.
U.S. officials reportedly monitored an audio feed of the Benghazi attack in real time, CBS News reports. FBI and State Department officials have also reviewed security camera footage recovered from the site. During the attack, U.S. forces were in place in nearby Sicily but the order to fly to Benghazi in an attempt to rescue the besieged staff at the consulate never came.
On FNC's Megyn Kelly
She had a reporter doing a 'walk-thru' of Benghazi fiasco!
#120483 Oct 25, 2012
And yet he remains an illegal alien.
#120485 Oct 25, 2012
http://www.wnd.com/2012/09/isr aeli-science-website-obama-bir th-certificate-forged/
"Facts trump speculation"
Since: Dec 08
#120486 Oct 25, 2012
Apparently, according to play law, operation of foreign law matters within the jurisdiction of the US.
Hee hee.
"Facts trump speculation"
Since: Dec 08
#120487 Oct 25, 2012
According to birfoon Play Law.
So what?
#120488 Oct 25, 2012
Sorry "dust mite", no proof contained in your post. Omammie is still an illegal alien. Step aside now. Next!
North York, Canada
#120489 Oct 25, 2012
"USA prepares world to Third World War
A member of the Russian State Duma Yevgeny Fyodorov shared his vision of the current situation in the world with Pravda."
Shame on you, believing a Russian from the Duma.(you don't even know what the Duma is) and Pravda and not your own government. You are dangerous because you are so easily duped.'Nuf said.
#120490 Oct 25, 2012
And yet he remains an illegal alien, just like you Mobarf.
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8 of 8 people found the following review helpful
This review is from: The Scarpetta Factor: Scarpetta 17 (Hardcover)
I have been a Cornwell fan, ( well, Scarpetta fan, I never really got on with the others ) ever since The Body Farm many years ago. I wait eagerly for each new novel and pre-order them as soon as I can. I did the same with this one, and have never been so disappointed. This is no Scarpetta novel the standard of previous ones. As the above reviews have put more eloquently than I ever could, the characters have no substance any more. And what I loved about the early Scarpetta novels, the forensic pathology part, is completely missing. I started this book over a month ago and I'm only up to page 345, this has never happened before, I normally read them in 2 days tops, they are usually "can't-put-down-able", sadly not this one. Even my husband commented "This one hasn't gripped you has it?"......erm, no, it hasn't. 345 pages in and basically nothing has happened, it's the most boring Scarpetta novel EVER. I will finish it, as I always finish a book, but it will be an effort. I sincerely hope Patricia goes back to her earlier winning formula or I'm afraid she is going to lose readers of what has been up to now, a fabulous series of books.
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Location: England
Top Reviewer Ranking: 1,292,600

subset: commoncrawl


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Mr Berghia
Show all reviews
Axe Africa / Lynx Africa by Axe / Lynx
I have to admit I really like this Axe fragrance. I only had several body sprays of this one (didn't find more concentrated versions) and I used to wear it a lot when I was (very) young. Unfortunetly, I have some bad memories with this one. I didn't overdo it, but I have smelled several people who seemed to spray half a can of Africa and other Axe scents and the stench was horrible. These memories have stopped me from buying any more Axe products.
01st September, 2011


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A daily roundup of the best free tutorials, patterns and craft how-tos from craft blogs and diy websites!
*Keep valuables out of sight with this closet hanger safe on Sew4Home.*Decorate a wood grain flask with Almost Makes Perfect.*Create watercolor hoop art with Crafts Unleashed.*Build a pallet charging station with A Piece Of Rainbow on Remodelaholic.*Learn how to make marbleized paper on Lines Across.*Whip up a baby doll peasant dress with a pattern and tutorial from Melly Sews.*Decorate moccasins with crystals and Quiet Lion.*Build a sawhorse coffee table with Rouge Engineer.*Learn how to make this leather loop key chain on M&J Trimming.*Hello Wonderful shares directions for this little girl's ballerina tote bag.*Diy a tie rack with Craftaholics Anonymous.*Give a laminate bookshelf a build in makeover with Jenna Burger.*Learn how to bury your ends on a quilting project on We All Sew.*Preshrinking: Learn the What, Why, When and How- Sew4Home*Assemble a copper pipe magazine rack with Homedit.*Sew a Bon Voyage duffel bag with a tutorial from Sew Can Do.


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Buffalo Bills linebacker Brandon Spikes, who spent his first four NFL seasons with the New England Patriots, took more shots at his former team Wednesday.
Spikes signed a one-year deal with Buffalo this offseason after a contentious end to his stint with the Patriots, who placed him on injured reserve in January.
The 26-year-old Spikes, who was critical of the Patriots in an interview last month with a Buffalo radio station, took to Twitter on Wednesday to rant against his former team.
Great games on the schedule for 2014, but it's going to be like Christmas to hand the #Patriots [2] #PatriotsNation [3] a L…twice! #BILLSMAFIA [4]
— BrandonSpikes51 (@BrandonSpikes51) April 9, 2014 [5]
it's icing on my cake to hand the #Patriots [2] two big L's this year. #justwatch [6]
— BrandonSpikes51 (@BrandonSpikes51) April 9, 2014 [7]
4 years a slave
— BrandonSpikes51 (@BrandonSpikes51) April 9, 2014 [8]
For more on this story visit: ESPN [9]


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The Prasar Bharati is a statutory independent body founded under the Act of Prasar Bharati and advance into force from 23 Nov, 1997. It is the Communal Mass Broadcaster of the nation. The purposes of public mass broadcasting are obtained in charges of Prasar Bharati Act by way of Doordarshan and All India Radio, which earlier were functioning as media sections under the Ministry of, I & B and since the above-said period advanced to be constituents of Prasar Bharati. In the present days, Prasar Bharati wants to appoint some person under Prasar Bharati Recruitment 2015 project. So we are giving basis useful information to our visitor find their eligibility as per desired position.
Sponsored Links
Prasar Bharati Recruitment 2015 for the 34 posts of News Reader: The recent notification commenced by the Prasar Bharati is valuable to invite applications for the 34 positions of News Reader. This request for the post gets attached with eligibility required as discussed below:
Age limit: Age that gets attached to candidates for having the qualifying nature is 21 years to 45 years. Age relaxation also gets permitted to the candidates that belong to category reserved.
Educational Criterion Set: Prasar Bharati had announced the educational obligation as graduation with subject English. Candidate must also be able to make the impressive conversation with the respective Indian language and must having appropriate voice tone to read the bulletins.
Selection Method: Written examination is set as the first step in making the selection of candidates. Aspirants got selected initially will commence for voice test and interview.
The Method of Registration: To get listed for the seat vacated Prasar Bharati had made available the layout of the application. This application is to send with the essential certificates under the covering scribed with name of the post on it.
Significant Date: 29th May 2015: The closing date that got commenced to send the application and hard copy of certificates.
References: Prasar Bharati with the linkage below had provided to get the recruitment notification as well as application form required to fill for the candidates.
Here is the linkage:
Download Recruitment Official Notification
Prasar Bharati Recruitment 2015 for the 24 posts of Broadcast Executive, Camera Man and other posts (Closed): National Academy of Broadcasting & Multimedia, Prasar Bharati, New Delhi had provided the orientation scheme. Applicants are needed to get their energy having leaning and slanting for the 24 posts of Broadcast Executive, Camera Man and other posts. The points of the eligibility are provided as below:
- Broadcast Executive Grade II: 06 Posts
- Camera Man Grade II: 05 Posts
- Creative Editor (Graphics): 01 Post
- Light Man: 04 Posts
- Post Production Assistant Grade I: 04 Posts
- Research Assistant: 02 Posts
- Web Content Manager: 02 Posts
Educational Criterion Set: Official notification of the Prasar Bharati is the need which is provided for the applicants to get so that the education under each of the post is being considered.
Scheme to submit an application: Prasar Bharati had mentioned the suitable process of the registration. Applicants as announced are provided that the application which is under the arranged format is being provided. This application is mentioned to tie up with the required certificates and is needed at next then to send with the necessary certificates. Applicants can also send the soft copy of the application at the mailing address as mentioned with the relavant certificates.
Important Date: 27th March 2015: Last date which is mentioned for the complete submission of application at the work place as mentioned by the supremacy. Applicants are being mentioned by the authority also that the applications after the date will not be considered at any cost and candidature of the applicants will be canceled.
References: The link which is necessary is provided beneath by the Prasar Bharati so that the notification which is necessary is get by the applicants
Download Recruitment Official Notification
Prasar Bharati Recruitment 2015 for the 8 posts of Post Production Assistant and Intern (Closed): Prasar Bharati, Directorate General Doordarshan, New Delhi had mentioned the necessary directives with which the staffing or enrollment of the applicants is being completed. The announcement is related with the 8 posts of Post Production Assistant and Intern and allocation of the seats is provide beneath:
- Post Production Assistant Grade – I: 06 Posts
- Interns (Content Manager): 02 Posts
Educational Criterion Set:
- Post Production Assistant Grade – I: Applicants are liable with the educational qualification to be as Professional diploma which is needed in the film & video editing with the familiarity with the post which is demanding for the minimum of 2 years.
- Interns (Content Manager): The educational necessity which is being concerning with the specific post is provided as PG degree or Diploma which is necessary in the journalism or production. Applicants having the education degree or diploma in the computer applications are qualified for the post.
Selection Method: The process of the making the short listing of the applicants will be commenced by the Prasar Bharati with the qualification and the experience which is being under the possession and aptitude of the applicants.
Scheme to submit an application: Under the process which is being associated with the registration it is being mentioned for the applicants to get through all the eligibility norms. Applicants are provided by the Prasar Bharati to use the prescribed application and send with the necessary certificates.
Important Date: 26th Dec 2014: Closing date provided so that the applications are being received at the office premises.
References: link which is being provided below by the Prasar Bharati is needed to obtain the official notification:
Download Recruitment Official Notification
Prasar Bharati Recruitment 2015 for the 15 posts of production Assistant, Library Assistant and other posts (Closed): Prasar Bharati, Indian Doordarshan is a very huge broadcasting institute. This promotes the real talent by the medium of the communication. To provide the helping hand in this regards the need is being felt for the 15 posts of production Assistant, Library Assistant and other posts. Detail associated is provided as below:
Educational Criterion Set: It is being provided for the applicants that the need of the education for each of the announced post must be obtained with the notification that is being structured by the Prasar Bharati. Applicants must thoroughly check all the detail and then decide on to apply for the post.
Scheme to submit an application: Prasar Bharati had provided with the process so that applicants could get registered. This is suggested only for the applicants that had been getting qualified as per the eligibility details. Applicants are required to make the forwarding of the application under the prescribed format and bio data. Applicants are needed to make the attachment of the essential certificates so that authority could make the count of the eligibility.
Important Date: Closing date which is mentioned for the applicants so as for reaching all the applications at the administrative center is 26th Dec 2014.
References: The link which is mentioned by the Prasar Bharati below is required to obtain the complete information:
Download Recruitment Official Notification
Sponsored Links


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Originally written on Fox Sports Kansas City | Last updated 4/12/14
MINNEAPOLIS -- Chris Colabello drove in three runs, Kyle Gibson pitched into the seventh inning and the Minnesota Twins beat the Kansas City Royals 10-1 Friday night to end a nine-game home losing streak that dated to last season. Josmil Pinto homered for the Twins, who reached double digits in runs for the third time this year. Mike Moustakas had two hits for Kansas City, which had won seven straight at Target Field. A 4-6 start to the season for Minnesota has been largely due to ineffective starting pitching. The Twins had received just two outings of six or more innings entering the game -- only one quality start -- and the other four starters besides Gibson have a combined 7.50 ERA. After a shaky first inning when he threw 28 pitches and gave up a run, Gibson (2-0) settled down and retired 14 of the next 18 batters. In 6 1/3 innings, he allowed five hits and one earned run while striking out three, lowering his ERA to 1.59. It was the second straight strong outing for the right-...
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Annual General Meeting, 24 August 2015, King's College London, London, UK
This year's annual general meeting will take place at King's College London on Monday, 24 August 2015 following a half-day symposium on palaeography. We will be holding elections for two new members of the board of directors, as Mr David Hirsch and Prof. Jan Just Witkam will have finished their terms. Read more →
New Course: Introduction to Islamic Codicology, 3–7 August 2015, Stanford University, Stanford, California, USA
This intensive five-day course will introduce the study of Islamic manuscript codices as physical objects, or the archaeology of the Islamic book; and is intended for art historians, bookbinders, codicologists, conservators, curators, and anyone else working with or studying Islamic manuscripts.
It will be taught by Professor Jan Just Witkam, Professor Emeritus of Codicology and Palaeography of the Islamic World at the University of Leiden and Editor-in-Chief of the Association's Journal of Islamic Manuscripts. Read more →
New Course: Introduction to Islamic Codicology, 7–11 September 2015, Cambridge University Library, Cambridge, UK
This intensive five-day course will introduce the study of Islamic manuscript codices as physical objects, or the archaeology of the Islamic book. Daily illustrated lectures will provide an overview of writing supports, the structure of quires, ruling and page layout, bookbinding, ornamentation, tools and materials used in book making, and the paleography of book hands.
It will be led by Professor François Déroche, holder of the Chair of the History of the Qur'an, Text and Transmission at the Collège de France and author of the book Islamic Codicology: An Introduction to the Study of Manuscripts in Arabic Script. Read more →
A Barakat Trust Scholarship is available for the full course. For details and to apply, see the Association's website here.
New Course: Islamic Manuscript Collections in Conflict Zones: Safeguarding Written Heritage, 5–7 October 2015, University of Cambridge, Cambridge, UK
This course will introduce the concepts and mechanisms that underpin cultural property protection in the present day and educate participants in best practices of managing, protecting, and preserving manuscript collections at risk. It is intended for academics, policy makers, cultural experts, lawyers and military experts, and will also be of interest to conservators, librarians, art historians, and other researchers working with Islamic manuscripts and written heritage in conflict areas. Read more →
A Barakat Trust Scholarship is available for the full course. For details and to apply, see here.
Journal of Islamic Manuscripts Added to Scopus
The Journal of Islamic Manuscripts was recently accepted for inclusion in Scopus, the world's largest abstract and citation database of peer-reviewed literature. Scopus is a bibliographic database containing abstracts and citations for academic journal articles. It covers nearly 22,000 titles from over 5,000 publishers, of which 20,000 are peer-reviewed journals in the scientific, technical, medical, and social sciences, including the arts and humanities.


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|Hepatitis B treatment
Apr 26, 2011
I was diagnosed ad Heb B positive since 1999. My diagnosis has been chronic hepatitis B e-antigen negativity, previously low HBV DNA and normal ALT but the latest test showed a marked increase in hepatitis B viral copies to greater than 10 million which equates to 1.4 million international units. A rise in my ALT to 83 iu/L has been noted. This was from the latest test in July 10. Three weeks ago, I started treatment with Temofovir. I feel fine, but these results have scared me. Any words of comfort?
| Response from Dr. McGovern
Your doctor has made a decision to treat you for your HBV infection based on level of viremia and perhaps concerns about chronic viral hepatitis. Tenofovir is a potent drug for HBV and to date has NOT been associated with ANY drug resistance. That is truly remarkable.
Continue to monitor your HBV laboratories and you should also get a blood test for alfa feto protein and imaging with an ultrasound every 6 to 12 months.
Your sex partner should also be tested and if negative should get vaccinated against HBV.
You should also get vaccinated against Hepatitis A if you are negative.
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- AP Source: Bulls reach 5-year, $90M deal with Jimmy Butler Updated: Jul-01-15 4:55 pm
CHICAGO - Jimmy Butler bet on himself and won big.
- Stephen Curry overtakes LeBron James for NBA's top jersey Updated: Jun-30-15 2:54 pm
OAKLAND, California - Stephen Curry has surpassed LeBron James for the most popular jersey in the NBA.
- Chicago Bulls take Arkansas' Bobby Portis with 22nd pick Updated: Jun-26-15 12:39 am
CHICAGO - The Chicago Bulls figured Arkansas power forward Bobby Portis would be gone by the time they picked. Once their turn came, the decision was easy.


text:
A probe for a surface-enhanced Raman scattering spectrometer is provided for injection into a cell in order to detect trace amounts of a compound within that cell. The probe has a spherical shape with a diameter less than one micrometer and preferably in the 10-500 nanometer range. The nanoprobes can have a receptor coating related to the specific compound to be detected by the probe. A process for producing, injecting and utilizing the nanoprobes are described.
1. A probe for a surface-enhanced Raman scattering spectroscopy, the probe comprising a body with a width less than one micrometer and a metallic coating which completely encapsulates the body and enhances Raman scattering during spectroscopic examination of a biological substance containing the probe.
2. The probe as recited in claim 1 wherein the body has a width in the range 10 to 500 nanometers.
3. The probe as recited in claim 1 wherein the body has a width in the range 10 to 100 nanometers.
4. The probe as recited in claim 1 wherein the body has substantially a spherical shape.
5. The probe as recited in claim 1 further comprises another coating selected from the group consisting of a bioreceptor and biomimetic material.
6. The probe as recited in claim 1 further comprises another coating selected from the group consisting of an antibody, an enzyme, a chemoreceptor, a microorganism, a tissue, an organelle and DNA.
7. The probe as recited in claim 1 wherein the body comprises a core of magnetic material surrounded by a metal coating.
8. The probe as recited in claim 1 wherein the body comprises a core of electrically charged material surrounded by a metal coating.
9. The probe as recited in claim 1 wherein the body comprises a first particle of metallic material abutting a second particle of non-metallic material.
10. The probe as recited in claim 9 wherein the first particle comprises material selected from the group consisting of a polymer, silica and titania.
11. The probe as recited in claim 9 further comprising a layer of material applied to one of the first particle and the second particle, wherein the material is selected from the group consisting of a polymer, a bioreceptor and biomimetic material.
12. The probe as recited in claim 9 further comprising a layer of material applied to one of the first particle and the second particle, wherein the material is selected from the group consisting of an antibody, an enzyme, a gene fragment, a chemoreceptor, a microorganism, a tissue, an organelle and DNA.
13. The probe as recited in claim 9 wherein the body comprises a core of non-metallic material and a plurality of metallic particles encrusted onto the core.
14. The probe as recited in claim 9 wherein the body comprises a core of non-metallic material enclosing a plurality of metallic particles.
15. The probe as recited in claim 13 further comprising a layer of material applied to one of the core and plurality of particles, wherein the material is selected from the group consisting of a polymer, a bioreceptor and biomimetic material.
16. The probe as recited in claim 1 further comprising another coating containing a gene fragment.
17. A method of surface-enhanced Raman spectroscopy comprising:
introducing a probe into a biological substance being examined, the probe comprising a body with a width less than one micrometer and having a metallic surface which enhances Raman scattering during spectroscopic examination;
exciting the biological substance with a source of radiation;
detecting the Raman emission emitted from the biological substance; and
spectroscopically analyzing the Raman emission.
18. The method as recited in claim 17 wherein the source of radiation provides excitation in the near infrared spectrum.
19. The method recited in claim 17 wherein the source of radiation provides excitation at two different wavelengths.
20. The method as recited in claim 17 wherein introducing a probe into a biological substance comprises introducing the probe into a cell.
21. The method as recited in claim 17 further comprising producing a probe by coating a core of metallic material with material selected from the group consisting of biomimetic material, an antibody, an enzyme, a chemoreceptor, a microorganism, a tissue, an organelle and DNA.
22. The method as recited in claim 17 further comprising producing a probe by applying a metal coating to a core of material selected from the group consisting of magnetic material and electrically charged material.
23. The method as recited in claim 17 wherein detecting the Raman signal uses a two-dimensional detector.
24. The method as recited in claim 17 further comprising filtering a signal produced by detecting the Raman emission.
25. The method as recited in claim 17 wherein the filtering employs a tunable filter.
26. The method as recited in claim 17 wherein the filtering employs a tunable filter, selected from the group consisting of an acousto-optic tunable filter and a liquid crystal tunable filter.
27. The method as recited in claim 17 wherein the metallic surface of the probe is formed by a metallic coating which completely encapsulates the body.
28. The method as recited in claim 17 further comprising producing a probe by coating a core of metallic material with a polymer.
29. The method as recited in claim 17 further comprising producing a probe by coating a core of metallic material with a gene fragment.
The present invention relates to Raman spectroscopy and surface-enhanced Raman spectroscopy; and more particularly to surface-enhanced Raman medical (SERMED) diagnostic instruments and methods for non-invasive medical diagnosis and drug screening.
Normal Raman spectroscopy relates to the scattering of light by a gas, liquid or solid with a shift in frequency or wavelength from that of the usually monochromatic incident radiation. Upon irradiation of a molecule with light in biological applications, the incident radiation having a frequency ν should produce scattered radiation, the most intense part of which has unchanged frequency (Rayleigh scattering). In addition, if the polarization of a molecule changes as it rotates or vibrates, there are spectral lines of much lesser intensity at frequencies ν�νk, where νk is the molecular frequencies of rotation or vibration.
Fleischmann et al. first reported strongly enhanced Raman scattering from pyridine molecules adsorbed on silver electrode surfaces that had been roughened electrochemically by oxidation-reduction cycles (Chem. Phys. Lett. 26, 163, 1974). This increase in Raman signal, originally attributed to a high surface density produced by the roughening of the surface of electrodes, was later identified by Jeanmaire and Van Duyne (J. Electroanal. Chem. 84, 1, 1977) and independently by Albrecht and Creighton (J. Am. Chem. Soc. 99, 5215, 1977) as a direct result of a surface-enhancement process, hence the term surface-enhanced Raman scattering (SERS) effect.
There are at least two major types of mechanisms that contribute to the SERS effect: a) an electromagnetic effect associated with large local fields caused by electromagnetic resonances occurring near metal surface structures, and b) a chemical effect involving a scattering process associated with chemical interactions between the molecule and the metal surface. It has been shown that electromagnetic interactions between the molecule and the substrate provide one of the dominant enhancements in the SERS process. Such electromagnetic interactions are divided into two major classes; interactions that occur only in the presence of a radiation field, and interactions that occur even without a radiation field. The first class of interactions between the molecule and the substrate are believed to play a major role in the SERS process. A major contribution to electromagnetic enhancement is due to surface plasmons. Surface plasmons are associated with collective excitations of surface conduction electrons in metal particles. Raman enhancements result from excitation of these surface plasmons by the incident radiation. At the plasmon frequency, the metal becomes highly polarizable, resulting in large field-induced polarizations and thus large local fields on the surface. These local fields increase the Raman emission intensity, which is proportional to the square of the applied field at the molecule. Additional enhancement is due to excitation of surface plasmons by the Raman emission radiation of the molecule.
Surface plasmons are not the only sources of enhanced local electromagnetic fields. Other types of electromagnetic enhancement mechanisms are concentration of electromagnetic field lines near high-curvature points on the surface, i.e., the "lightning rod" effect, polarization of the surface by dipole-induced fields in absorbed molecules, i.e., the image effect, and Fresnel reflection effects.
The chemical effect is associated with the overlap of metal and adsorbate electronic wave functions, which leads to ground-state and light-induced charge-transfer processes. In the charge-transfer model, an electron of the metal, excited by the incident photon, tunnels into a charge-transfer excited state of the adsorbed molecule. The resulting negative ion (adsorbate molecule-electron) has a different equilibrium geometry than the original neutral adsorbate molecule. Therefore, the charge-transfer process induces a nuclear relaxation in the adsorbate molecule which, after the return of the electron to the metal, leads to a vibrationally excited neutral molecule and to emission of a Raman-shifted photon. The "adatom model" also suggests additional Ramon enhancement for adsorbates at special active sites of atomic-scale roughness, which may facilitate charge-transfer enhancement mechanisms.
A general object of the present invention is to provide a surface-enhanced Raman spectroscopic technique, that increases Raman emission due to the surface-enhanced Raman scattering effect and can be used inside microsize structures, such as cells.
Another object is to provide a probe for such a technique which can be delivered into a biological, chemical or physical structure to provide surface-enhanced Raman emission. The SERS effect and its applications have been reviewed by T. Vo Dinh, "Surface-enhanced Raman spectroscopy using metallic nanostructures", Trends in Analytical Chemistry, 1998.
A further object of the present invention is to provide such a probe which is less than one micrometer in size.
Yet another object is to provide methods for injecting the probe into such microscopic structures.
These and other objectives are satisfied by a probe for a surface-enhanced Raman scattering monitor or spectrometer which is suited to detect trace quantities of toxic chemicals and related biological indicators. The nanometer size of these probes allows them to be delivered inside organisms and even a single cell to serve as intracellular self-contained sensors, thereby extending the usefulness and application of the SERMED probes to the realm of intracellular medical diagnosis, as well as extra-cellular diagnosis.
The nanoprobe of the present invention comprises a metallic system which provides the SERS effect and a chemical/biological system which provides selective binding within the cell. The nanoprobe has a metallic core which optionally may be magnetic or electrically charged materials. For example the core may be solely metallic material or a non-metallic material with a metallic coating. Preferably the core has an external coating formed of a polymer, a biological material (such as an antibody, enzyme or DNA) or biometric material (e.g. PNA, cyclodextrins or molecular imprint). A nanoprobe can be constructed to sense a particular characteristic of the cell by having specific receptors that provide diagnostic information of different regions and species inside the cell. The receptors also can be selected to provide information regarding characteristics outside of the cells, on the outside surface of the cell, or inside the cells near the nucleus or other intracellular component.
Multiple nanoprobes can be used in high throughput screening for drug detection or medical diagnostics, whereby a large number of single cells can be analyzed simultaneously, each cell or group of cells can be analyzed simultaneously by one or more nanoprobes.
FIGS. 1A through 1G are cross sectional views of several embodiments of a surface-enhanced Raman scattering probe according to the present invention;
FIGS. 2A through 2D show alternative processes for introducing the probe into a biological cell;
FIG. 3 is a block diagram of a spectral imaging surface-enhanced Raman scattering instrument; and
FIG. 4 illustrates a probe according to the present invention being irradiated by a light beam.
The present surface-enhanced Raman medical (SERMED) diagnostic system utilizes extremely small probes to enhance Raman emission in order to detect trace material within a cell. Preferably the probes are spherically shaped, although other shapes may be employed. Because cell sizes range from one to ten micrometers, the present SERMED probes for intracellular use are 10 to 100 nanometers in diameter. For extra-cellular use, larger nanoprobes have typical diameters in the 30 to 500 nanometer range are used, but such probes can be up to substantially one micrometer in diameter. Because the probe typically is smaller than one micrometer (i.e. submicron), it is being referred to herein as a "nanoprobe".
With reference to FIGS. 1A-1G, the nanoprobe can have one of several embodiments depending upon the specific application for the probe. In the basic form shown in FIG. 1A, the nanoprobe comprises a spherical metal core 10 thus providing a probe with a metallic surface. As an alternative, this version of the nanoprobe can have a non-metallic core 12 with an outer metallic coating 14.
Another embodiment of the nanoprobe is illustrated in FIG. 1B and has a metallic core 16 covered with a coating 18, 20 or 22 which provides selective binding to the cell structure. The coating can comprise a polymer 18, or a bioreceptor 20, such as an antibody, a cell component, an enzyme or DNA. In addition, the external coating around the metal core 16 can comprise a biomimetic material 22.
With reference to FIG. 1C, the nanoprobe may be formed by a magnetic core 24 with an outer coating of a metal 26 that induces the SERS effect. Another variation is the use of electrically charged material for the core 28 about which a similar metal coating 30 is applied. Magnetic or electrically charged cores allow remote manipulation of the nanoprobe using magnetic or electric fields to guide the nanoprobe inside the cells, and then to specific locations therein. Similarly the magnetic or electric field can be employed to remove a nanoprobe from the cell.
FIG. 1D illustrates the use of a magnetic core 32 having an external metal layer 34 about which an outer coating of a chemical or biological material 36 is applied. A variation of this structure utilizes a magnetic core 38 with a metal intermediate layer 40 over which a chemical coating 42 has been applied. Another variation of the basic structure of a magnetic core 44 and a metal coating 46 utilizes an exterior coating of a bioreceptor 48.
The chemical nanoprobe coatings include materials, such as polymers and chemical functional groups, that facilitate delivery and transport of the nanoprobes inside the media being investigated. A bioreceptor is employed to identify the target compound of the investigation via molecular recognition. The bioreceptors may be an enzyme, an antibody, a gene fragment, a chemoreceptor, a tissue, an organella, or a microorganism. The operation of the antibody probes is based on the antibody—antigen molecular recognition, and DNA probe operation is based on the hydridization process. Hydridization of nucleic acid probe to DNA biotargets (e.g., gene sequences, bacteria and viral DNA) offers a high degree of accuracy for identifying DNA sequences which are complimentary to that of the probe.
FIG. 1E depicts another nanoprobe configuration in which a metallic nano-particle 50 is adjacent to submicron non-metallic (receptor) material 52. For example the non-metallic material 52 may be a polymer, silica or titania. Variations of this configuration can coat either the metallic particle 50 or the non-metallic material 52 with a chemical or biological coating 54, previously described.
Referring to FIG. 1F, submicron metallic particles 56 also can be encrusted onto non-metallic material 58 to form the nanoprobe. Similarly a chemical or biological coating 60 of a bioreceptor or biomimetic material may be applied to the metallic particles 56 or the non-metallic material 58.
FIG. 1G depicts yet another embodiment of nanoprobes consisting of metallic nano-particles formed inside a non-metallic particle 62 with a chemical or biological coating 63. Alternatively a biometric material 64 may be applied.
The preparation of these nanoprobes involves depositing submicron (nano-sized) particles onto a substrate, such as a glass plate. The particles can comprise polystyrene latex spheres, fumed silica, titanium oxide or aluminum oxide particles. For example, this deposition is accomplished by placing a glass slide on spin coating device. A few drops of the submicron particles in a water solution is placed on the glass slide which is then immediately spun to distribute the material across the surface of the glass slide. The spinning precludes clumping of the particles on the glass surface and provides uniformly adhered coverage. The coverage of the particles can be controlled by varying the concentration of the nanoparticle solutions.
The second step in the process involves coating the particle-covered glass slide with silver. This is accomplished using a vacuum evaporator and in which the pressure in the evaporator during coating is less than 5�10−6 torr. The rate of silver deposition is controlled between 1.5 and 2.0 nm/sec. The deposition rate and thickness of the silver can be measured with a Kronos model QM-311 quartz crystal thickness monitor. The nanospheres coated with silver are removed from the substrate and are ready for use as nanoprobes.
Among the techniques based on solid substrates, the methods using a simple submicron material, such as fumed silica, Teflon� or latex spheres, appear to be the simplest to prepare. Teflon� and latex spheres are commercially available in a wide variety of sizes. The shapes of these materials are very regular and their size can be selected for optimal enhancement of the SERS effect.
A 50 microliter volume of a suspension of latex or Teflon� submicron spheres was applied to the surface of a substrate. Various substrates were utilized, including filter paper, cellulosic membranes, glass plates and quartz materials. Next the substrate was placed on a high speed spinning device and spun at 800 to 2,000 RPM for 20 seconds. Silver then was deposited on the nanosphere coating in a vacuum evaporator at a deposition rate of 2 nm/second to form a silver layer having a thickness of 5 to 100 nm.
Titanium oxide was coated with silver as an alternative material for use with SERS. This technique was found to produce efficient Raman enhancement. Commercially available titanium oxide particles of the appropriate size were first deposited on glass and cellulose substrates in a water suspension of 10% concentration by weight. The titanium oxide particles then were coated with a 50 to 100 nm layer of silver by the previously described thermal evaporation process. The silver coated titanium oxide particles were removed from the substrate and found to be ready for SERS use.
Another type of nanoprobe material that is quite SERS active and easy to prepare is fumed silica based material. Fumed silica has been used as a thickening agent in various industrial processes and is commercially available. The fumed silica particles were suspended in a 10% water solution and coated onto a glass plate. The particles were coated with a 50 to 100 nm of silver by thermal expansion and then removed from the substrate. This technique also can be utilized to produce silver coated alumina nanoprobes.
Silver colloid hydrosol also produces an SERS active media in solution. Such colloid hydrosol has the advantage of ease of colloid formation and straight forward characterization of the colloid solutions by simple UV absorption. Silver colloids are generally prepared by rapid mixing a solution of AgNO3 with ice-cold NaBH4. Such colloid systems tend to coagulate which may adversely affect their use as nanoprobes. However, that coagulation problem can be minimized by stabilizers, such as poly(vinylalcohol), poly(vinylpyrrolidone) and sodium dodecyl sulfate. Nevertheless, use of such stabilizers can produce interferences and cannot be utilized inside cellular environments. A method that can minimize the coagulation problem involves coating the system with a monolayer of a chemical, such as a polymer, immediately after nanoparticles of silver sols are formed to stabilize their size and shape.
With respect to chemical and biological exterior coatings of the nanoprobes, DNA oligonucleotides can be attached readily since most of the SERS coatings are based on gold or silver. The binding of oligonucleotides to such metal surfaces can be based on thiol chemistry or other standard chemical binding methods. The thiols are known to strongly chemisorb to gold and silver surfaces to form monolayers that possess supramolecular properties. Peptide nucleic acid (PNA) can be used instead of DNA.
If the over coat of the nanoprobe is silica, the DNA probe is bound to the silica coating. The silica surface is derivatized with silan by incubation in a 2% 3-aminopropyl triethoxysilane (APTS) for 24 hours at room temperature, washed in acetone and dried in a vacuum. The silanyl groups are activated by incubation in 1% gutaraldehyde in water for one hour at room temperature. Excess gutaraldehyde is removed by washing in water and rinsing with phosphate buffered saline (PBS). The DNA probe molecules containing amino linkers are attached to the silica surface by incubating for 24 hours at 4� C. with a probe solution (e.g. concentration of 10 mg/mL). The unbound probe is washed away with PBS.
An alternative approach is to overcoat the SERMED nanoprobe surface with a thin layer of polymer. In this case, various bioreceptors can be bound to the polymer coating. Biomimetic material, such as cyclodextrins or molecular imprint, can be attached to such nanoprobes.
Several methods can be employed to deliver the SERMED nanoprobes inside cells. With reference to FIG. 2A, SERMED nanoprobes in a solution can be injected through a micro injector 70 by applying appropriate pressure. Alternatively, if the nanoprobes are magnetic, such as those with magnetic cores, a magnetic field can be applied which drives the nanoprobes into the cell 72. If the nanoprobes are electrically charged, a voltage can be employed that propels the nanoprobes into the cells as an ionic current, a technique which is referred to as iontophoresis.
Another technique is illustrated in FIG. 2B in which a pressurized microjet 74 of an inert gas such as helium, containing an emulsion of nanoprobes, is used to inject the nanoprobes into the cell.
With reference to FIG. 2C another injection technique involves making the cell membrane transiently permeable to the outside solution containing the nanoprobes. This is accomplished by disrupting the membrane structure with a brief, but intense, electrical shock. For example, a pair of electrodes 76 and 78 are placed on opposite sides of the cell and 2,000 volts per centimeter is applied across the electrodes for 200 microseconds. This allows some of the nanoprobes to migrate into the cell and become retained there following the shock.
FIG. 2D depicts membrane bound vesicles 80 that are loaded with nanoprobes 82. The vesicles are induced to fuse with the target cell 84 at which point the nanoprobes migrate into the cell.
After the nanoprobes have been introduced into the biological material to be studied, a Raman imaging system 100, such as the one in FIG. 3, is used to measure the SERS signals. The Raman imaging system 100 includes, for example, a 5 mW helium-neon laser 102 that produces an output beam 104 having a wavelength of 632.8 nm. The laser beam 104 passes through a bandpass filter 106 and then is expanded and recollimated using a spacial filter/beam expansion module 108. The resultant beam 110 enters a microscope system 112 where it is directed through the sample 114 containing the SERMED nanoprobes. In one embodiment, sample 114 may be a multiple microcell sample holder with each microcell containing a separate sample for high throughput drug screening or biomedical analysis. The light emitted by the sample 114 travels to an objective lens 116 that has an appropriate magnification. The objective lens focuses the light onto a beam splitter 118 which divides the light and sends a portion through a first exit port 120 of the microscope system 112.
The light from the first exit port passes through a holographic notch filter 122 which rejects the laser scatter and through an optical filter 123 that transmits the Raman signal. Filter 123 may be a bandpass filter or a tunable filter, such as an acousto-optic or liquid crystal tunable filter. The resultant beam is sent to a charge-coupled device (CCD) video camera 124 that produces a two-dimensional image which is presented to the operator on a video monitor 126. The CCD camera 124 is operated by a control system 128. The nanoprobe technology is very appropriate for high throughput analysis since nanoprobes can be used to analyze single cells. Therefore, very number of single cells can be analyzed in parallel. For example, the CCD camera with 1000�1000 pixels allows 106 simultaneous analyses.
The other portion of the light from beam splitter 118 passes through a second exit port 128 of the microscope system 112 and is used to detect the SERS spectra from the sample 114. That portion of the light passes through a second holographic notch filter 130 to reject the laser scatter from the SERS emission and the resultant beam is focused through an optical fiber 132 by appropriate optics 134. Additional optics 136 at the output of the optical fiber 132 focuses the light onto the emission slit of a spectrograph 138, such as model HR320 manufactured by ISA. The spectrograph 138 is equipped with a thermo-electrically cooled, red-enhanced intensified CCD imager 140, such as model RE/ICCD 5765 manufactured by Princeton Instruments. If two-dimensional multi-spectral images are not required, a conventional Raman spectrometer may be used.
The use of SERMED nanoprobes extends the usefulness and application areas of SERS techniques considerably. Medical applications involve all the techniques that analyze intracellular environment inside single cells. In addition, SERMED nanoprobes can be ejected or implanted into a living organism to provide in situ self-contained nanoprobes to monitor the function of that organism.
Appropriate chemical receptors or biological receptors can be designed so that the nanoprobes bind to specific targets inside cells. Electric or magnetic fields may be employed to move the nanoprobes (with magnetic cores) into desired environments inside cells. As shown in FIG. 4, the nanoprobe 150 adjacent cell nucleus 151 is irradiated by a light beam 152, such as from a laser outside the cell 154. Due to the short range of the SERS effect induced by the nanoprobes only cellular components absorbed on or near the nanoprobes experience the SERS effect, thereby producing the SERS signal 156 that is received by a detector 158.
An important advantage of the SERMED nanoprobe is the non-invasive method of excitation and detection. For example, near-infrared (NIR) excitation can penetrate tissue to excite the nanoprobe inside cells and tissue. The resulting SERS signal (red-shifted in wavelength) also is inside the NIR range so as to be emitted from the cells and tissue.
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Posts with tag "jenny mccarthy"
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Patti Stanger is single and ready to mingle. Although 'The Millionaire Matchmaker' star is usually the one setting up other people on dates, she's back "in the trenches" looking for love and ready to host her own mixer. "I'm single. It has its great moments [and] its scary moments. I'm nervous just like anybody else," she tells PopEater about entering the dating world again. In addition to ...
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Outspoken actress and activist Jenny McCarthy is back in the dating game, and she's not shy about sharing the good news. The former Playboy Playmate, who dated actor Jim Carrey for five years before breaking things off in early 2010, has found love once again with sports agent Paul Krepelka. While McCarthy tells Ellen DeGeneres that she's "really happy" with Krepelka, who she met on a blind ...
Jenny McCarthy Splits from Boyfriend of Four MonthsPosted Dec 7th 2010 at 10:00 PM by Tracey Harrington McCoy
- Filed under:
- Famous Couples -
- Splitsville
Attention all single guys: former Playboy bunny Jenny McCarthy is back on the market! The actress-activist-author and her boyfriend of four months, Jason Toohey, have split, E! Online.com reports. "It's true," McCarthy's rep told the website. The former couple began dating early this summer and even shared a house together in Las Vegas. Toohey, a former fitness model, resides in Las Vegas and o...
Jenny McCarthy's Breakup Came While Writing 'Love, Lust and Faking It'Posted Oct 11th 2010 at 10:03 AM by Nicki Gostin
- Filed under:
- Sex That's SFW -
- Splitsville -
- TV News -
- Interviews
In Jenny McCarthy's brazenly outrageous and brutally honest new book, 'Love, Lust and Faking It: The Naked Truth About Sex, Lies and True Romance,' the TV personality and New York Times bestselling author bares all about her failed romances, love of vibrators and boob jobs. But the book isn't just for laughs -- it also offers up some serious advice on relationships and achieving happiness. What...
Jenny McCarthy Reveals Details About Jim Carrey Break UpPosted Sep 27th 2010 at 07:05 PM by PopEater Staff
- Filed under:
- Movie News -
- Splitsville
Hollywood was shocked earlier this year when funnyman Jim Carrey and funny lady Jenny McCarthy announced they were breaking up--and for the first time since the split, McCarthy is sharing some insight into what happened. The Playboy model-turned-actress-turned-author discusses the relationship on an episode of 'The Oprah Winfrey Show' that airs Tuesday, PEOPLE reports. McCarthy visits Oprah'...
Is Jenny McCarthy's New Boyfriend a Facebook Jerk?Posted Aug 22nd 2010 at 04:00 PM by Jo Piazza
- Filed under:
- Famous Couples -
- Pop-Ed
Pop-Ed: Maybe we're just calling this one like we see it, but Jenny McCarthy's new man seems just a little bit jerky. We're basing this entirely on former fitness model Jason Toohey's Facebook profile which was creepily still public even after his elevated social status hit the tabloids. The first issue is Jason's concerning breach of social media dating ettiquette by rushing headlong into rela...
Jenny McCarthy Has a New BoyfriendPosted Aug 17th 2010 at 04:05 PM by PopEater Staff
- Filed under:
- Famous Couples -
- TV News
Sorry guys -- Jenny McCarthy is officially off the market. The former Playboy model is dating Jason Toohey, a former fitness model, her rep confirmed to PEOPLE. The pair have been dating for about two months -- and already live together. The couple have a house in Las Vegas, and the actress seems to be enjoying her time in Sin City. Over the weekend, McCarthy played hostess at the Midsummer Nigh...
Photos: Jenny McCarthy Parties in Sexy LingeriePosted Aug 16th 2010 at 03:10 PM by PopEater Staff
- Filed under:
- Gut Reactions -
- Movie News -
- Photos -
- Sex That's SFW
Former Playboy model Jenny McCarthy dusted off one of her raunchiest outfits to host The Palms' 4th Annual Midsummer Night's Dream party in Las Vegas this weekend. The 37-year-old actress donned a tiny white bikini, which left very little to the imagination. Guess she wanted to show funnyman Jim Carrey what he's missing out on. "I have one more year to pull off an outfit like this, so I better h...
Jim Carrey's Antics Keep Us GuessingPosted Apr 28th 2010 at 06:45 AM by Ben Widdicombe
- Filed under:
- Movie News -
- Splitsville -
- Celebrity Scandal
If you've been wondering what's been on Jim Carrey's mind since his recent split with Jenny McCarthy, the answer to that is "Boing." Since April 24, Carrey has been tweeting the word up to 40 times day, using it to mean, well, just about anything. "I RRRefuse 2 define BOING!," he wrote on Tuesday. "1 day when you've given up your need 2 explain it and file it neatly away, 'BOING'... you will un...
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Scottrade in the News
Either Rebalance Your Retirement Portfolio or Risk Capsizing Your FuturePublished: 06/10/2015
Jason Notte, TheStreet
Scottrade's Joe Correnti discusses portfolio rebalancing to help long-term investors stay on track.
The Daily Brief - Of Love, Marriage and FinancesPublished: 02/12/2015
Staff in The Daily Brief, WealthManagement.com
Scottrade's Joe Correnti discusses recent Scottrade client survey results on love and money and the importance of defining long-term financial goals in a relationship.
Scottrade Aims To Be More Than A Discount BrokerPublished: 01/23/2015
Ken Shreve, Investor's Business Daily
Scottrade was recognized for overall customer satisfaction on IBD's list of Best Online Brokers. Hear from Scottrade's Erik Merkau about how the company is meeting client needs by expanding beyond low-cost trading to offer full-service solutions.
As Economic Headwinds Subside, Businesses Increasingly Reach Out for FinancingPublished: 12/12/2014
Leasing News
Scottrade Bank Equipment Finance President Fred Van Etten talks about the future of lending in an improving economy.
Frightening Financial Future Scares Investors Just Before HalloweenPublished: 10/14/2014
Jason Notte, Main St.
A recent Scottrade client survey reveals long-term caregiving for loved ones and healthcare top investors' financial fears.
A sneaky way to save more for retirementPublished: 9/25/2014
Reporter Kelli Grant discusses how health-expenses can affect retirement plans.
Bull run collision with correction likely in 2015, Scottrade surveyPublished: 9/5/2014
Adam Shell, USA Today
Scottrade's Brian Bachelier shares survey data that reveals clients' market sentiment and expectations for the bull market.
Scottrade Moves to Take On 'Big Four' CustodiansPublished: 9/3/2014
Charles Paikert, Financial Planning
Scottrade's Matt Wilson and Brian Stimpfl discuss investments made to Scottrade® Advisor Services to better serve registered investment advisors.
Scottrade, too, needs for brokers here and elsewherePublished: 8/29/2014
Greg Edwards, St. Louis Business Journal
Scottrade is looking to hire investment consultants across its nationwide branch network.
Look who's in the stock market now: MillennialsPublished: 7/20/2014
Stephanie Landsman, CNBC
Scottrade's Brian Bachelier discusses the generational differences among traders. Based on results from the 2014 Scottrade American Trader Study, Millennials are willing to take on additional risk and are more likely to use social analysis.
Recent News Releases
- Scottrade Senior VP Named YWCA Leader of Distinction
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Message: No More Excuses by Nick Holden of Truth Impacts Min - Today's Christian Videos
Embed truthimpacts 2152 views 2010-10-01T22:55:07 Message: No More Excuses (Romans 12:9-11) by Nick Holden of Truth Impacts Ministries. We have no excuse why we do not take the lead in love, holiness, and brotherly affection. We are called to lead as the conquerors of the Lord who always leads us into triumph. (2 Cor. 2:14) www.truthimpacts.org or www.truthimpacts.blogspot.com. Remember, Jesus Paid it ALL!!! (Heb. 13:20-21)
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Subject: MS artist/author C. A. Webb Announces THREE shows to close out the year 2004!
On behalf of C. A. Webb, Inc.'s board of directors, thanks you for making 2004 a year that we will not soon forget. With 26 days left in the year, we are going to give you what we have always strived to share: our best________________________________________________________Showtime at Jackson and Shadow Play Entertainment welcome you to the 1st Annual MS Winter Festival for the Arts!Place: MS Arts Center (201 E. Pascagoula) When: Friday, December 10, 2004Come enjoy…4:30-6:00p.m., Open ForumThis will be an opportunity for all those interested in the music industry to ask questions and interact with those who are living the arts daily. Cleveland Barnes (Showtime at Jackson), Kehinde Gaynor (music producer and graphic designer), a representative for Malaco Records and Mr. "Sho Nuff" KAMIKAZE will be discussing self-promotion, the ins and outs of contracts, royalties, financial empowerment, distribution, how to be a part of musical compilations and television production. And then at 7:00p.m….No need to dress up, but come see the host of entertainers that will be assembled from all genres to give Jackson, MS a show to end all shows in 2004! The evening begins with open mic poetry and featured poet Donovan Scott. Then settle in to enjoy R&B performers Salima, Duane Scott and Sho', blues musician Scott Albert Johnson, and hiphop heavyweights Lil' Bone, Next of Kindredz (N.O.K.)and theJack-Town Ballers, headlined by KAMIKAZE! After the concert, get ready to dance the night away until midnight! During the entire evening, free food and drinks will be available as well as many opportunities to network and meet new people. Venders will be on hand with merchandise from area businesses (including illustrator Chuck Galey and the owners of The Cookie Net), and Shadow Play Entertainment will have its unique products available including their new line of greeting cards! Doors open at 4p.m. Admission to the forum or concert is $10.00 or purchase an all-day pass for only $15.00! All ages are welcome, so come enjoy this evening of the Arts with something for the entire family! * This event is sponsored by Showtime at Jackson, Shadow Play Entertainment, C. A. Webb, Inc., MS Arts Commission, Amsouth Bank, and Malaco Records. _______________________________________________________Shadow Play Entertainment welcomes you to EMANCIPATION!Saturday, December 11, 2004Smith Robertson Museum 3-6p.m. There will be concession-style food for sale, games and prizes for the kids, art work on display by Webb, sketching by Wez Harglo and other local artists. Performances will take place from Elijah and Ellison Brown, inspirational singer Martha Simpson as well as local talent right from Mississippi.7:30-10p.m.Webb concludes the day with the main program: EMANCIPATION: Free to Be. There will be open mic for all, performances from guest poets Kira and Donovan Scott, R&B singers Duane Scott and Sho, blues musician Scott Albert Johnson, Jackson's Finest Next of Kindredz and more! Admission to the day's event is FREE, however, 10% of all proceeds raised by Shadow Play Entertainment will benefit Building Bridges Inc. and Southern AIDS Commission Food Pantry. * Shadow Play Entertainment is the trademarked subsidiary of C. A. Webb, Inc. Fire and Ice is sponsored by Shadow Play Entertainment and co-sponsored by the City of Jackson and the Smith Robertson Museum. ________________________________________________________ MS artist/author C. A. Webb and Shadow Play Entertainment will present "Webb Wednesday at Santiago's." Wednesday, December 15, 2004 @ 8p.m.(Santiago's is located at 101 South St. at the corner of South St. and Farish St.)This will be a night of positive and enlightening open mic for poets, singers and musicians alike. This will be a night of positive open mic for poets, singers and musicians alike. The features of the night will be guest poets Donovan Scott and Kira, performers Scott Albert Johnson, Duane Scott, and Jackson's Finest, Next of Kindredz (N.O.K.). There will be door prizes, a drawing for a free weekend in a Jacuzzi suite compliments of C. A. Webb and Best Western Executive Inn and two drawings for $50.00 CASH to be given away that night!! Food and drinks will be available. Artists will have merchandise available for purchasing. This is a night where Hiphop, reggae, and other musical genres will be celebrated. The guest D.J. will be Mr. C-Lecta of WMPR 90. 1 FM. Admission to this event is $7.00 in advance and $10.00 at the door. ADVANCE TICKETS CAN BE PURCHASED AT THE BEST WESTERN EXECUTIVE INN, DOWNTOWN JACKSON UNTIL Wednesday, December 15, 2004 at 2P.M. (601.969.6555) Doors open at 6:30p.m. This is a night where poetry, hiphop, reggae, and other musical genres will be celebrated... Don't miss it! The guest D.J. for the evening is Mr. C-Lecta of WMPR 90.1 FM. You can also contact Santiago's at (601) 354-3030._______________________________________________________ For more information about any of the above, contact Cyrus A. Webb at (601) 201-8139 or visit www.cawebb.com.
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The LiarPenguin Publishing Group 2015; US$ 27.95
The extraordinary new novel by the #1� New York Times ?bestselling author of� The Collector . � Shelby Foxworth lost her husband. Then she lost her illusions ? � The man who took her from Tennessee to an exclusive Philadelphia suburb left her in crippling debt. He was an adulterer and a liar, and when Shelby tracks down his safe-deposit box, she... more...
The LiarLittle, Brown Book Group 2015; Not Available
When her husband Richard dies in a freak accident, Shelby Pomeroy is devastated. But his death reveals a horrible truth - Richard was a liar and a cheat. Now Shelby is left with the consequences - huge, terrifying debts and mounting proof that her late husband betrayed her in every conceivable way. Heart-broken but unbowed, Shelby is determined... more...
Brazen VirtueRandom House Publishing Group 2010; US$ 7.99
Praise for #1 New York Times bestselling author Nora Roberts: ?Her stories have fueled the dreams of twenty-five million readers.?? Entertainment Weekly ?You can?t bottle wish-fulfillment, but Nora Roberts certainly knows how to put it on the page.? ? The New York Times ?Roberts is indeed a word artist, painting her stories and her characters... more...
Nora Roberts's Circle TrilogyPenguin Publishing Group 2010; US$ 23.99
#1 New York Times bestselling author Nora Roberts delivers an extraordinary trilogy?an epic saga that breaks down the boundaries between reality and the otherworldly, while forging together the passions of the men and women caught in a battle for the fate of humanity? As a storm rages, the tale begins?of a powerful vampire?s lust for destruction?and... more...
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AVAILABLE DIGITALLY FOR THE FIRST TIME The last thing Preston McQuinn wants is a nosy neighbour. He wants peace, quiet and as little distraction as possible while he works on his new play. That's why he's in New York - where people leave each other alone. But Preston hadn't factored in cartoonist Cybil Campbell, the girl across the hall. Cybil... more...
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When her husband Richard dies in a freak accident, Shelby Pomeroy is devastated. But his death reveals a horrible truth - Richard was a liar and a cheat. Now Shelby is left with the consequences - huge, terrifying debts and mounting proof that her late husband betrayed her in every conceivable way. Heart-broken but unbowed, Shelby is determined... more...
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TrodesJackFlip Releases SoundShielder - An Automatic Volume Control for Streaming Media
TrodesJackFlip, a new software company located in Minnetonka, MN, has released its first software application - SoundShielder - that addresses the troublesome loudness variations in streaming media, such as loud advertisements, low dialog levels in movies, or unexpected volume differences when switching sources.
With the explosive growth of on-demand content, more consumers turn to their PC to be entertained. Though each source has a different volume level, SoundShielder provides one volume for all sources and can remove the jumps in audio levels when switching from a news blurb on CNN©, a movie on Netflix©, or when streaming live television or music to your headphones. SoundShielder makes real-time, automatic volume adjustments to the incoming audio source, aimed at user defined, target listening levels.
"Our goal was to create an application that solved a big problem initially - the loud commercials! But the application works for movies, TV, music and more. Early in our testing, we also had moms use the app to protect their kid's hearing when playing games," says Tom Greenwalt, Co-founder of TrodesJackFlip and CTO. "Users can now set one volume for everything and it will never be too loud," he added.
SoundShielder has an MSRP of $4.95 and is available online at http://www.soundshielder.com, including a download link for a fully functional 14 day trial copy. The product is certified to run on Windows 7, VISTA and Windows 8. A Mac version is expected to ship in Q3 of 2014.
Find us on FaceBook: http://www.facebook.com/SoundShielder.
About TrodesJackFlip
SoundShielder is a product developed by TrodesJackFlip - a software company in Minneapolis, Minnesota who's goal is to create affordable, problem solving applications that provide the best possible value and utility.
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Ty Pennington Style Parkside Wicker Gazebo 10' x 12'
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AKC Saint Bernard male. He is 7 months old. He will come with full AKC papers. He is up to date on his shots. He is good around children and other dogs. He is $1000. If you are interested please email.
Jul 3 -
Asheville :
Maggie Valley - Dogs & puppies
Ckc Saint Bernard puppies born June 11 2015. I have 2 boys and 3 girls. Mom and Dad both ckc, good with kids and other pets. Puppies will be ready July 23, 2015. Shots and Dewormed will be up to date .Vet check before going to new home. Please call for more information @ 704-535-9009 or Text me @ 980-23-7109(NOW TAKING DEPOSITS $250)
Jun 18 -
Charlotte :
Charlotte - Dogs & puppies
We have a beautiful litter of saint babies available! Mom dad both on site , all babies have been vaccinated and dewormed and ready now to go to their forever home ...
We raise our babies right along with our other pets they are not caged or left alone at anytime.. They are included in our family as one of my own ... Around children and cats .
We have 4 boys and 2 girls :)
I can drive to ...
Jun 9 -
Charleston (WV) :
Belle - Dogs & puppies
May 20 -
Charlotte :
Charlotte - Dogs & puppies
CKC Saint Bernard puppies. We have 6 males and 3 females. These guys are perfect for Farther's Day presents. Mom and dad on site. Mom weighs in at approximately 160lbs and dad 150lbs. Mom has a beautiful 7 generation pedigree with ancestors from Switzerland. These guys will come with their first shots, scheduled wormings, CKC application, and a puppy starter kit. Puppies are raised indoors so ...
May 16 -
Richmond :
Montpelier - Dogs & puppies
I have an AKC registered Saint Bernard boy named Winston not quite 2 years old who needs a new home. He is very energetic and extremely friendly with people and other dogs. Loves to play and extremely likes belly rubs. He needs a large fenced yard to run an play. He gets a little too excited with children and his big paws can accidentally knock them down. He is good around livestock and ...
May 15 -
Augusta :
Greenwood - Dogs & puppies
We'll be sending text message alerts for your subscription to


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Game Stats
Game Stats
W: R. Halladay (8-3) L: J. McDonald (3-4)
|1st Inning
Neil Walker (8) 2 run shot to center off Halladay, Paul scored.
|4th Inning
Ibanez hit sacrifice fly to center, Utley scored.
|5th Inning
Howard hit sacrifice fly to center, Victorino scored, Polanco to third, Utley to second.
Ruiz hit by pitch, Polanco scored, Utley to third, Ibanez to second.
|6th Inning
Polanco singled to left, Halladay scored.
|8th Inning
Howard singled to center, Polanco scored, Utley to second.
|8th Inning
McCutchen singled to left, Harrison scored.
|9th Inning
Victorino singled to center, Valdez scored, Francisco to second.
Howard singled to right, Francisco scored, Victorino to third.


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Belmont (7.35 mile)
July 11 , August 1
Learn a little more about nature this season with Daniel Stowe Botanical Garden's educational Wild About Summer Series, featuring three specific Garden facets this June, July and August.
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July 19
The largest display ever staged at Daniel Stowe Botanical Garden - ZimSculpt - returns this May to July with more than 100 displayed sculptures reflecting world-renowned Shona art.
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July 17 , July 31
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Friday Night Live concerts are free and held every other Friday evening from May 22 until August 28, 7-10 p.m, and are free to attend.
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Lowell (0.07 mile)
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Southern cooking along with Steaks, Chicken and Fish and also, sandwiches. Eat in or carry out.
Lowell (0.08 mile)
A variety of delicious cakes to choose from! Special event and wedding cakes can be ordered also.
Lowell (0.45 mile)
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Award winning barbeque pork and ribs, steaks and chicke, all cooked on an open pit. We do catering as well from 10 to 5,000 people.
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Hard to beat our location! Truck parking, Bank of America Stadium (16 miles), Bobcats Arena(16 miles), Carowinds (20 miles), Charlotte Motor Speedw...
Gastonia (2.56 mile)
The newly remodeled Comfort Suites Hotels is ideally located minutes from Charlotte & offers convenient access to Interstate 85, US 74, & Hwy 321. ...
Gastonia (2.59 mile)
The Fairfield Inn offers a continental breakfast, indoor pool, non-smoking rooms, handicap access, adjacent restaurant, meeting rooms, exercise fac...
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