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Publications (20)50.14 Total impact
- SourceAvailable from: Jia Xiao
Article: Jagged-1 signaling suppresses the IL-6 and TGF-β treatment-induced Th17 cell differentiation via the reduction of RORγt/IL-17A/IL-17F/IL-23a/IL-12rb1[Show abstract] [Hide abstract]
ABSTRACT: Jagged-1 signaling has recently been reported to be involved in the Th17 cell differentiation. However, little is known about its mechanisms. Soluble Jagged-1 was used to activate the Jagged-1-Notch signaling to interfere with the IL-6 and TGF-β-induced Th17 cell skewing. Genes relevant to the autoimmunity or inflammation were screened for the first time in this system by qPCR array for the differential expressions. The 18 genes out of 84, including Clec7a, Il12b, Il12rb1, Il12rb2, Csf3, Il15, Il17a, Il17f, Il17rc, Il17rd, Il17re, Il23a, Myd88, Socs1, Stat4, Stat5a, Sykb and Tbx21, were downregulated, but only Cxcl2, Cxcl12 and Mmp3 were upregulated. The expressions of the genes, Rorγt, Il17a, Il17f, Il12rb1 and Il23a, induced by simultaneous IL-6 and TGF-β treatment were significantly suppressed by Jagged-1, followed by the reduction of RORγt, IL-17A, and IL-17F. Consistent with the attenuation of RORγt, and the reduced production and secretion of IL-17A and IL-17F in the cell supernatant and the in situ stained cells, the number of CD4(+)IL-17(+) cells was also diminished. It is concluded that the Jagged-1-Notch signaling can suppress the IL-6 and TGF-β treatment-induced Th17 cell skewing through the attenuation of RORγt and, hence by, the down-regulation of IL-17A, IL-17F, IL-23a, and IL-12rb1.Scientific Reports 02/2015; 5:8234. DOI:10.1038/srep08234 · 5.58 Impact Factor
Article: PI3K pathway inhibitor LY294002 alters Jurkat T cell biobehaviours via ERK1/2-ICBP90 mediation[Show abstract] [Hide abstract]
ABSTRACT: Little is known about whether there is a relationshipbetweenPI3K/AKT, ERK1/2 and an inverted CCAAT box binding protein (ICBP90) in biological behaviours of tumour cells. The aim of this study was to determine thisusing Jurkat T cells. Compared to PD98059 (an ERK1/2 signaling inhibitor), DAPT (a Notch signaling inhibitor) or adriamycin (a classical anti-tumour drug), the inhibition of Jurkat T cell growth by LY294002 (a PI3K/Akt signaling inhibitor) was more obvious. LY294002 combined with adriamycin appeared to have a synergistic effect. LY294002 strongly blocked Jurkat T cells at each phase of cell cycle with a decrease of DNA content, superior to adriamycin. Consistent with these changes, the expression of phosphorylated ERK1/2 was markedly decreased in the LY294002-treated Jurkat T cells, leading to the reduction of ICBP90 production, followed by moderate attenuation of TGF-β secretion. The results suggest that deactivation of PI3K/Akt signalling can surpress Jurkat T cell growth through inhibiting cell proliferation and blocking the cell cycle. ICBP90 may mediate the PI3K/AKT-ERK1/2 signalling to regulate leukemia cell growth.Central European Journal of Biology 08/2014; 9(8). DOI:10.2478/s11535-014-0313-2 · 0.63 Impact Factor
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ABSTRACT: Anisomycin is a pyrrolidine antibiotic isolated from Streptomyces griseolus. It has been found that a quite low dose of anisomycin is sufficient to block proliferation of primary T lymphocytes. The focus of this study is to explore the possibility of anisomycin to treat human acute leukemia Jurkat T cells in vitro. The results indicated that the low dose of anisomycin could significantly inhibit the colony formation of Jurkat T cells and elevate the inhibition rate of Jurkat T cell growth along with its increasing concentrations. Jurkat T cell cycle was blocked into S-phase by anisomycin. Consistent with the increased proportion of sub-G1 phase, anisomycin promoted Jurkat T cell apoptosis. The CD69 and CD25 expression on the surface of Jurkat T cells was also down-regulated prominently along with the enhancing concentrations of anisomycin, followed by the decreased production of IL-4, IL-10, IL-17, TGF-β and IFN-γ, and the down-regulated expression of phosphorylated-ERK1/2. The results suggest that the suppressive effect of anisomycin on Jurkat T cell growth may be related to inhibiting TGF-β production and ERK1/2 activation, arresting the cell cycle at S-phase and promoting the apoptosis of Jurkat T cells.Central European Journal of Biology 12/2013; 8(12). DOI:10.2478/s11535-013-0235-4 · 0.63 Impact Factor
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ABSTRACT: Ionomycin in conjunction with phorbol-12,13-dibutyrate (PDBu) is conventionally used as a stimulator to activate cells, especially original T cells. But we accidently found it had an entirely opposite action on malignant tumor cells derived from T cells. Thus, influence of ionomycin on human leukemia Jurkat T cell behaviors and its preliminary mechanistic process were explored in the presence of PDBu. Ionomycin could remarkably inhibit colony formation of the cells, and inhibitory rate of the cell proliferation was increased with ionomycin treatment in a dose- or time-related relationship, following the reduction of ERK1/2 and phosphorylated-ERK1/2 levels. However, a high dose of ionomycin might moderately repress mid-stage activation of the cells. It also blocked the cell entry at S-phase and G2/M-phase with the attenuation of transforming growth factor-β (TGF-β) level in the cells, and promoted the cell apoptosis following the augment of caspase-3 and cleaved caspase-3 in the cells. The dramatic elevation of [Ca2(+)]i and intracellular pH (pHi) was simultaneously followed by the above alteration of the cell behaviors. These results indicate that ionomycin may strongly inhibit human acute T lymphocyte leukemia progress in the presence of PDBu through the inhibition of ERK1/2 signaling, the activation of caspase-3 and the attenuation of TGF-β mediated by the [Ca2(+)]i and pHi enhancement, providing a novel insight into function and potential application of both ionomycin and PDBu.Annals of Hematology 11/2013; 93(5). DOI:10.1007/s00277-013-1955-2 · 2.40 Impact Factor
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ABSTRACT: Activation of Notch by Jagged-1 may plays a pivotal role in maturation of dendritic cells (DCs), but the mechanism has not been completely defined. In the present study, Hes-1 (Hairy/enhancer-of-split)-targeting siRNA was used to confirm a role of Jagged-1-Notch signaling pathway activation in maturation of murine bone marrow-derived DCs and to search for a target that plays a critical role. The results showed that compared with the control, lipopolysaccharide or Zymosan A groups, Jagged-1 (a soluble Jagged 1/Fc chimera protein) effectively increased expression of Hes-1 and Deltex-1 mRNA, which could be reversed by DAPT (2, 4-diamino-5-phenylthiazole), a specific inhibitor of the Notch signaling pathway. Hes-1-targeting siRNA could successfully down-regulate the endogenous Hes-1 expression in the DCs. Concurrently, a significant down-regulation of CD40, CD80, CD86 and MHC-II expressions on the surface of the DCs was found with the reduction of IL-12 yielded by the DCs. Our results demonstrate that Hes-1-targeting siRNA can inhibit the maturation of the DCs induced by Jagged-1, indicating Hes-1 may be an important target of Notch signaling mediating the maturation of DCs.Central European Journal of Biology 11/2013; 8(11). DOI:10.2478/s11535-013-0231-8 · 0.63 Impact Factor
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ABSTRACT: Anisomycin eminently inhibits cell proliferation in vitro. The aim of this study was to explore the potential of anisomycin to treat tumors in vivo and its mechanism(s) of action. The results showed that peritumoral administration of anisomycin significantly suppressed Ehrlich ascites carcinoma (EAC) growth resulting in the survival of approximately 60% of the mice 90 days after EAC inoculation. Enhancement of infiltrating lymphocytes was noted in the tumor tissue, which was dramatically superior to adriamycin. The growth inhibitory rate of EAC cells was enhanced with increasing concentrations of anisomycin, following an enhanced apoptotic rate. The total apoptotic rate induced by 160 ng/ml of anisomycin was higher when compared to that induced by 500 ng/ml of adriamycin. DNA breakage and nanostructure changes were also noted in the EAC cells. The levels of caspase-3 mRNA, caspase-3 and cleaved-caspase-3 proteins in the anisomycin‑treated EAC cells were augmented in a dose- and time-dependent manner, following the activation of caspase-8 and caspase-9, which finally triggered PARP cleavage. The cleaved-caspase-3, cleaved-caspase-8 and cleaved-caspase-9 proteins were mainly localized in the nuclei of the cells. These results indicate that anisomycin efficaciously represses in vitro and in vivo growth of EAC cells through caspase signaling, significantly superior to the effects of adriamycin. This suggests the potential of anisomycin for the treatment of breast cancer.Oncology Reports 03/2013; 29(6). DOI:10.3892/or.2013.2355 · 2.19 Impact Factor
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ABSTRACT: Background: Recent studies have shown that anisomycin significantly inhibits mammalian cell proliferation, but its mechanism remains unclear. In this study, Jurkat T cells were used to first explore a relationship between effect of anisomycin on them and alteration of cell cycle-regulating proteins. Methods: Cell colony formation, CCK-8 assay, flow cytometry, RT-PCR and western blot were employed to evaluate correlation of ten cell cycle-regulating proteins with suppression of the cell proliferation and arrest of the cell cycle by anisomycin. Results: Our data showed that anisomycin inhibited the colony-formation and proliferation of Jurkat T cells in a dose-dependent manner, and arrested the cells into S and G2/M phases with the production of sub-diploid cells. The levels of P21, P-P27 and P53/P-P53 reached their peaks 4 h after anisomycin treatment, presenting a positive correlation with anisomycin concentration, and P16, P-P21, P27, P57, P73/P-P73 and P-Rb changed little with the prolonged exposure time or increased concentrations of anisomycin. But the level of Rb protein was increased at 24 h after the treatment of anisomycin. The expression of an inverted CCAAT box binding protein (ICBP90) in Jurkat T cells came to decrease 12 h after the treatment of anisomycin, presenting a negative correlation with anisomycin concentration. Subsequently, the expression of P-CDK2 was also decreased at 24 h, presenting an obviously negative correlation, whereas P-CDK1 showed no differences among the differently treated Jurkat T cells. Furthermore, the level of P21 and P53 mRNA was increased with the enhanced concentrations of anisomycin. Conclusion: The results indicate that anisomycin may activate the P53/P21/P27 signaling to decrease the expression of ICBP90, inhibit expression of P-CDK2 to block the cells into S and G2/M phases, and finally result in proliferation inhibition of Jurkat T cells.Pharmacological reports: PR 03/2013; 65(2):435-44. DOI:10.1016/S1734-1140(13)71019-3 · 2.17 Impact Factor
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ABSTRACT: Notch signaling plays an important role in differentiation of T cells. However, little is known as to action of it in differentiation of Th17 cell subset. In this study, a soluble Jagged-1/Fc chimera protein (Jagged-1) was directly used to activate Jagged-1Notch signaling, while Hes-1-targeting siRNA was used to knock down Hes-1 gene to investigate effect of Jagged-1Hes-1 signaling on the differentiation of CD4+ T cells into Th17 cells. The results showed that Jagged-1 could promote the expression of Hes-1 and Deltex-1 mRNAs and the expression of NICD, Hes-1 and Deltex-1 proteins, which might be significantly blocked by DAPT, a specific inhibitor of Notch signaling. Jagged-1Hes-1 signaling resulted in the markedly decreased in situ expression of RORgammat in the CD4+ T cells induced by IL-6 plus TGF-ß. Flow cytometric analysis showed the reduction of IL-17 production in CD4+ T cells by Jagged-1, but the enhancement of it by Hes-1-targeting siRNA. The level of IL-10 produced by the treated cells was also enhanced, whereas the expression of IL-17 was prominently attenuated, which could be offset by anti-Jagged-1 antibody or DAPT. The results indicate that Jagged-1Hes-1 signaling can suppress the skewing of CD4+ T cells toward Th17 cells via RORgammat, for which Hes-1 may be crucial.Journal of biological regulators and homeostatic agents 01/2013; 27(1):79-93. · 2.41 Impact Factor
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ABSTRACT: Toll-like receptor (TLR)-TLR cross talk is thought to be important in TLR signaling. Herein, we investigated the effect of specific TLR3 and TLR7 agonists, poly (I:C) and R837, individually and in combination, on uterine immune cell function and their subsequent effects on pregnancy outcome. Allogeneic pregnancies in the non-obese diabetic (NOD) mousexC57BL/6 and wild-type BALB/cxC57BL/6 model were used. An additive increase in embryo resorption was observed after induction with both poly (I:C) and R837, and was associated with elevated numbers of both TNF-alpha- and IFN-gamma-producing CD45(+) cells in the uterus. Further examination showed that while cytokine expression was detected in both CD3(+) cells and CD49b(+) cells in BALB/c mice, NOD mouse cells behaved differently. In NOD mice, elevated cytokine expression was attributed to CD3(+) T cells, with no response detected in the CD49b(+) NK cells. The additive effect of combined agonists was partially inhibited by the Jun N-terminal kinase (JNK) mitogen-activated protein kinase (MAPK) inhibitor SP600125 and almost completely abrogated by the extracellular signal-regulated kinase (ERK) MAPK inhibitor PD98059. These results suggest that increased TLR3 and TLR7 signals are transmitted via Th1-type T cells, rather than NK cells, in NOD mice. Furthermore, the ERK MAPK pathway may be critical in TLR3 and TLR7 signaling.Journal of Reproductive Immunology 07/2009; 82(1):12-23. DOI:10.1016/j.jri.2009.03.004 · 2.37 Impact Factor
Article: Prevention of embryo loss in non-obese diabetic mice using adoptive ITGA2(+)ISG20(+) natural killer-cell transfer.[Show abstract] [Hide abstract]
ABSTRACT: Both regulatory T cells and regulatory natural killer (NK) cells may play essential roles in the maintenance of pregnancy. In this study, we show that a significantly high percentage of spontaneous embryo loss was observed in both allogeneic and syngeneic pregnant non-obese diabetic (NOD) mice. The percentage of embryo loss in allogeneic pregnant mice was further increased by the administration of anti-asialo ganglio-N-tetraosylceramide to deplete NK cells, but was decreased by the adoptive transfer of ITGA2(+)ISG20(+) (CD49b(+) CD25(+)) NK cells from normal mice. No such trend was observed in syngeneic pregnant NOD mice. The pattern of CXCR4 (specific receptor for CXCL12) expression on NK cells was analyzed and NK-cell migration was confirmed by in vitro and in vivo migratory assays. Since CXCL12 production by murine trophoblast cells was confirmed previously, our findings suggest that the recruitment of peripheral CXCR4-expressing ITGA2(+)ISG20(+) NK cells into pregnant uteri may be important in the regulation of feto-maternal tolerance.Reproduction 04/2009; 137(6):943-55. DOI:10.1530/REP-08-0412
Article: Improvement of fertility with adoptive CD25+ natural killer cell transfer in subfertile non-obese diabetic mice.[Show abstract] [Hide abstract]
ABSTRACT: To investigate the role that CD25(+) natural killer (NK) cells may play in the establishment of pregnancy, the effect of adoptive CD25(+) NK cell transfer on pregnancy outcome in subfertile non-obese diabetic (NOD) mice was investigated. Phenotypic analysis of NK cells was performed by flow cytometry before and after the transfer. The proportion of subfertile NOD female mice that failed to become pregnant when co-caged with C57Bl/6 males for 16 weeks was significantly higher in female NOD mice than in normal female BALB/c controls (53.1% versus 15.1%; P < 0.01). The subfertile NOD mice were divided into three groups receiving CD25(+) NK cells (group 1), CD25(-) cells (group 2) or RPMI 1640 medium only (group 3). Group 1 had significantly more pregnancies than those receiving CD25(-) NK cells (77.8% versus 11.1%; P < 0.01) and controls injected with RPMI 1640 medium (0.0%; P < 0.01). Improved fertility was concomitant with an increase in placental CD49b(+) NK cells expressing Foxp3. Foxp3 expression was confirmed in the CD25(+) NK cells before the transfer. These results indicate that subfertility in NOD mice may be partially attributed to the insufficiency of CD25(+) and Foxp3(+) NK cells recruited into the pregnant uterus.Reproductive biomedicine online 01/2009; 18(1):95-103. DOI:10.1016/S1472-6483(10)60430-0 · 2.98 Impact Factor
Article: Comparison of murine thymic stromal lymphopoietin- and polyinosinic polycytidylic acid-mediated placental dendritic cell activation.[Show abstract] [Hide abstract]
ABSTRACT: We confirmed previously the existence of thymic stromal lymphopoietin (TSLP)-positive cells in murine placenta by flow cytometry. To compare the characteristics of Toll-like receptor 3 (TLR3)- and TSLP-mediated placental dendritic cell (DC) activation, pregnant BALB/c mouse mated with C57BL/6 male were used as a model of allogenic gestation. Placental CD11c(+) DCs were potently activated by the TLR3-agonist polyinosinic polycytidylic acid [poly (I:C)], subsequently causing increased expression of co-stimulatory molecules. Accordingly, increased intracellular production of interleukin (IL)-12 and interferon (IFN)-gamma, but not IL-4 or IL-10, were detected after stimulation by poly (I:C). In the case of TSLP-stimulation, although increased expression of co-stimulatory molecules was also detected, there was no substantial increase of intracellular production of IL-12, IFN-gamma, IL-4 or IL-10. In contrast, the expression of the Th2 cell-attracting chemokine, the thymus and activation-regulated chemokine (TARC) or CCL17, was significantly boosted in response to TSLP induction, whereas no significant increase of CCL17 was observed when triggering TLR3 with its specific agonist poly (I:C). The data were further supported by a CD4(+)IL-10(+) cell migratory assay. These results suggest that TSLP-TSLP receptor interaction may result in a Th2-type microenvironment at the feto-maternal interface by inducing the production of Th2 cell-attracting chemokine and modulating the immigration of Th2-type cells.Journal of Reproductive Immunology 01/2009; 79(2):119-28. DOI:10.1016/j.jri.2008.10.001 · 2.37 Impact Factor
Article: Anisomycin Inhibits the Behaviors of T cells and the Allogeneic Skin transplantation in mice[Show abstract] [Hide abstract]
ABSTRACT: There is still a lack of a high potent and low toxic immunosuppressive drug. We accidentally found that a quite low dose of anisomycin was sufficient to block proliferation of T cells. In this study, carboxy-fluorescein diacetate-succinimidyl ester staining showed that over 10.0 ng/mL of anisomycin markedly inhibited the proliferation of T cells induced by ConA. Propidium iodide staining revealed that anisomycin led to G0/G1 arrest and blocked S phase entry stimulated by ConA or phorbol 12, 13-dibutyrate plus ionomycin. Anisomycin down-regulated remarkably the CD69 and CD25 expression on the surface of T cells. The response of T cells was repressed by treatment of anisomycin, which was partly restored by adding exogenous interleukin-2, and there was no difference between anisomycin and dexamethasone, although the used dose of the latter was 100-fold of the former. The inhibition of cytotoxicity of T cells against 7919 cells by anisomycin was observed without the direct cytotoxicity to T cells or 7919 cells. The level of transforming growth factor-beta1 fell by <80.0 ng/mL in vitro and 30.0 mg/kg of anisomycin in vivo and enhanced by more than the doses. The treatment of anisomycin prolonged the survival of the transplanted skin and depressed the delayed type hypersensitivity development and the T-cell response in the skin-transplanted mice. Moreover, the effect of its restraining allograft rejection might be superior to cyclosporine A, with relatively slight toxic signs. These results indicate anisomycin significantly inhibits the behaviors of T cells and the transplantation rejection, providing important evidence for anisomycin as a novel immunosuppressant.Journal of immunotherapy (Hagerstown, Md.: 1997) 10/2008; 31(9):858-70. DOI:10.1097/CJI.0b013e3181869873 · 3.35 Impact Factor
Article: Characterization of natural killer cells in nonobese diabetic/severely compromised immunodeficient mice during pregnancy.[Show abstract] [Hide abstract]
ABSTRACT: To characterize uterine natural killer (uNK) cells in nonobese diabetic/severely compromised immunodeficient (NOD/SCID) mice and investigate the potential role of these cells in pregnancy tolerance. An animal model-based study. Academic research center in a university. Syngeneic pregnant NOD/SCID mice were compared with non-immunodeficient BALB/c mice. Induction of Toll-like receptor (TLR) agonists. Flow cytometric analysis was performed to detect the percentage of cell subsets, and standard (51)Cr release assay was performed to determine cytotoxicity. The dominant subset of uNK cells in NOD/SCID mice is DX5 (CD49b)(+), asialo ganglio-N-tetraosylceramide(+), CD25(+), CD122(+), Thy-1 (CD90)(hi), c-kit (CD117)(hi), and interleukin-10(+). In addition, the percentage of interferon-gamma(+) subset was slightly increased in response to selected TLR agonists in the NOD/SCID mice, whereas the corresponding percentage in BALB/c mice could be increased dramatically. Such an effect could be abrogated by inhibitors, including LY294002, SP600125, and PD98059. The significant increase of interferon-gamma(+) NK cell percentage in BALB/c mice was concomitant with the increase of the embryo resorption rate. In contrast, the resorption rate in NOD/SCID mice was not significantly increased upon the induction of polyinosinic polycytidylic acid or lipopolysaccharide. As expected, the NK cells from NOD/SCID mice display a detectable but lower cytotoxicity than BALB/c, as determined by standard (51)Cr release assay. In addition, the uNK cells from NOD/SCID mice also display a hyposensitivity to lipopolysaccharide-induced production of inducible nitric oxide synthase. A considerable percentage of immature NK cells were detected at the fetomaternal interface in NOD/SCID mice. These cells were hyposensitive to the stimulation of selected TLR agonists. Such a status seemed to be beneficial for the maintenance of pregnancy.Fertility and sterility 05/2008; 91(6):2676-86. DOI:10.1016/j.fertnstert.2007.08.087 · 4.30 Impact Factor
Article: TSLP-induced placental DC activation and IL-10(+) NK cell expansion: comparative study based on BALB/c x C57BL/6 and NOD/SCID x C57BL/6 pregnant models.[Show abstract] [Hide abstract]
ABSTRACT: Thymic stromal lymphopoietin (TSLP)-TSLP receptor (TSLP-R) interactions activate CD11c(+) dendritic cells (DCs) and increase epithelial cell Th2-type cytokine production. We detected intracellular TSLP expression on CK7(+) trophoblast cells and TSLP-R expression on placental DCs from pregnant BALB/cxC57BL/6 and NOD/SCIDxC57BL/6 mice on gestational day 12.5. Murine recombinant TSLP activated DCs from BALB/c mice, with increased CD80 and CD83 expressions; TSLP-activated DCs induced IL-10-producing NK cell expansion. This was abrogated by anti-TSLP Ab or by culturing CD49b(+) NK cells alone. No TSLP-DC-induced IL-10(+)CD49b(+) cell expansion occurred when DCs and CD49b(+) cells were cultured separately. Although TSLP-induced DC activation occurred in NOD/SCID mice, the IL-10(+) NK cell percentage was unchanged. CK7(+) trophoblast cells may activate placental DCs via a TSLP-TSLP-R interaction and induce DC-dependent placental NK cell IL-10 production. TSLP-DC and NK cell contact appears necessary for IL-10(+)CD49b(+) cell expansion. Placental NK cells from NOD/SCIDxC57BL/6 mice appear less sensitive to TSLP-DC induction.Clinical Immunology 02/2008; 126(1):104-17. DOI:10.1016/j.clim.2007.09.006 · 3.99 Impact Factor
Article: Potential role of toll-like receptor 3 in a murine model of polyinosinic-polycytidylic acid-induced embryo resorption[Show abstract] [Hide abstract]
ABSTRACT: To investigate the potential role that toll-like receptor 3 (TLR3) may play in a murine model of polyinosinic-polycytidylic acid (polyIC)-induced embryo resorption. An animal model-based study on induced embryo resorption. University animal laboratory. Pathogen-free animals housed under barrier conditions and monitored for health status. Pregnant BALB/c mouse mated by C57BL/6 male was used as a model of allogeneic gestation. The administration of polyIC was performed to establish a murine model of induced embryo resorption, with or without TLR3 blocking by multiple injection of mAb against this receptor. Flow cytometric analysis was performed to detect the percentage of CD45(+)DX5+ and DX5(+)CD69+ cell subsets, with the DX5 antigen used as a common natural killer (NK) cell marker, and CD69 as a marker for activated NK cells, respectively. In the allogeneic mating model, BALB/cxC57BL/6, both the CD45(+)DX5+ and DX5(+)CD69+ cell percentages were significantly elevated upon polyIC stimulation at the absence of anti-TLR3 administration but were kept unchanged if the female mice were pretreated with anti-TLR3 monoclonal antibody. Accordingly, the resorption rate of embryos was boosted by polyIC administration, but this effect could be abrogated by pretreatment of anti-TLR3 mAb. The engagement of polyIC with TLR3 may be critical to the activation of NK cells infiltrated at the feto-maternal interface, subsequently resulting in an increase in embryo resorption.Fertility and sterility 05/2006; 85 Suppl 1:1125-9. DOI:10.1016/j.fertnstert.2005.08.056 · 4.30 Impact Factor
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ABSTRACT: The purpose of this study was to outline the fertility features of non-obese diabetic (NOD)/LtSz-scid/scid (NOD/SCID [severe combined immunodeficiency] for short) mice, and to evaluate the effects of NK cell subsets on the pregnancy outcomes of the syngeneic NOD/SCID x NOD/SCID mating combination. Firstly, lymphocyte phenotyping was performed with flow cytometry to confirm the multiple immunodeficits in NOD/SCID mice. Fertility features were assessed in NOD/SCID x NOD/SCID mice and compared with non-immunodeficiency control BALB/c x BALB/c mice. Although the presence of NK cell deficit is apparent in NOD/SCID mice, a certain level of remnant NK activity could be observed in these mice. The remnant NK cell activity was stimulated with polyinosinic-polycytidylic acid (polyIC), or inhibited with anti-asialo GM1 (ASGM1) anti-serum, respectively. The effects of these factors on pregnancy outcomes were evaluated after administration. Roughly normal fertility could be observed in NOD/SCID x NOD/SCID mice. However, a slightly larger sized litter was observed in polyIC-treated NOD/SCID x NOD/SCID mice compared with control NOD/SCID mice. In contrast, embryo resorption was boosted after ASGM1 injection, and correlated subsequently with a smaller litter size. It indicates that the remnant NK cell activity in NOD/SCID mice may be beneficial to feto-maternal tolerance during pregnancy.Journal of Reproductive Immunology 01/2006; 68(1-2):39-51. DOI:10.1016/j.jri.2005.05.002 · 2.37 Impact Factor
Article: Following the fate of murine epidermal stem cells in a syngeneic dermal equivalent in vivo.[Show abstract] [Hide abstract]
ABSTRACT: Stem cells in healthy epidermis have the capacity to produce structures, such as interfollicular epidermis, hair follicle, and sebaceous glands. Embryonic stem (ES) cells can be induced to differentiate into epidermal stem cells in vitro. These ESCs (epidermal or epidermoid stem cells derived from ES cells) have the potential to be an ideal replacement of those stem cells destroyed in severe injury, such as in deep and extensive burn. The aim of this study was to follow the fate of murine ESCs which were seeded in a syngeneic dermal equivalent and implanted into syngeneic recipient mice subcutaneously. ES cells were induced in vitro to differentiate into ESCs. Stained with a fluorescent dye Hoechst 33342, these ESCs were seeded into a fibroblast-collagen-gelatin sponge complex, functioning as a dermal equivalent model, and then implanted subcutaneously into 129/J mice, which were syngeneic to these stem cells. These ESCs were clearly visible in the implant by fluorescent microscopy 3 weeks or longer after implantation. These cells remained viable, differentiating into hair follicle-like structures, glandular structures, and gave rise to additional structures resembling native dermis. A number of markers were expressed in the differentiated structures, including CD29 (integrin beta1 subunit) and cytokeratin 18 (CK18). No apparent rejection or severe side effects were observed at least during the 10 weeks following implantation. Now that ESCs can survive in vivo in this dermal equivalent model and differentiate into hair follicle-like structures as well as glandular structures, it is feasible to use these cells as seed cells in studies to fabricate dermal equivalents that have the potential to develop dermal appendages.Burns 01/2006; 31(8):1007-12. DOI:10.1016/j.burns.2005.06.020 · 1.84 Impact Factor
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ABSTRACT: Cytokeratin 7 (CK7) is currently regarded as the best marker for trophoblast cells, while CD200 (OX-2), known as 'tolerance signal', plays an important role in normal pregnancy. In this study, the status of CD200 expression was investigated in BALB/c x C57BL/6 and BALB/c x BALB/c mating combinations designed as allogeneic and syngeneic murine models of induced embryo resorption, in which the resorption rate was boosted by an i.p. injection of poly (I:C), a synthetic double-stranded RNA. The percentage of CD200+ cells in the CK7+ cell population (CD200+ CK7+ percentage) and the absolute number of these cells were determined with flow cytometry, using trophoblast cells collected at day 8.5 and day 13.5 of gestation. The potential effect of poly (I:C) on CD200 expression was also evaluated by detecting the CD200+ CK7+ percentage in trophoblast cells incubated in the presence or absence of poly (I:C), in vitro. The distribution pattern of CD200+ cells at the feto-maternal interface was evaluated by immunocytochemical examination. When 10(4) cells were analyzed at day 8.5 of gestation in each case, no significant difference was observed between the poly (I:C)-treated group and the control PBS group either in the CD200+ CK7+ percentage or in the absolute number of these cells. Similar results were observed both in BALB/c x C57BL/6 mice and in BALB/c x BALB/c mice. However, the CD200+ CK7+ percentage was significantly decreased in the poly (I:C)-treated group when evaluated at day 13.5 of gestation. Accordingly, a dramatically elevated rate of embryo resorption was observed at this time point of pregnancy after the administration of poly (I:C). In addition, the CD200+ CK7+ percentage was significantly lower in trophoblast cells incubated with poly (I:C) at a certain concentration, in vitro, while histocytochemical examination showed the CD200+ cells mainly scattered in placental tissue adjacent to the interface of the placenta and uterus. This indicates that sufficient expression of the CD200 molecule on CK7+ cells at the feto-maternal interface may be necessary for the maintenance of embryos during pregnancy in this rodent model, while poly (I:C) administration may increase embryo resorption, at least partially via direct inhibition of CD200 expression on CK7+ cells.Reproduction (Cambridge, England) 11/2005; 130(4):529-37. DOI:10.1530/rep.1.00575 · 3.26 Impact Factor
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ABSTRACT: The present study aims to address whether the analysis of CD45+CD86+ cells isolated from para-aortic lymph nodes (pLNs) is valuable in assessment of the status of local immunity at the murine feto-maternal interface. CBA/J x DBA/2 mice, virgin CBA/J mice, and CBA/J x BALB/c mice were used as an abortion-prone model (group A), nonpregnant controls (group N), and fertile controls (group F), respectively. The percentage of CD45+CD86+ cells in the CD45+ cell group (CD45+CD86+ percentage for short) and the absolute number of these cells were determined by means of flow cytometry (FCM), using mononuclear cells isolated from pLNs collected 5.5, 9.5, and 13.5 days post-coitum (dpc), respectively, and mononuclear cells isolated from placentas 13.5 dpc. To clarify the identity of these CD86+ cells, FCM was also performed with CD3, CD19, and DX5 as specific markers for murine T-cells, B-cells, and NK cells, respectively. Both resorption rate and absolute number of resorptions were significantly higher in group A (29.3%, 1.8+/-1.0) than in group F (4.8%, 0.3+/-0.5, P<0.001, respectively). Similarly, both cell percentage and absolute number of CD45+CD86+ cells in pLNs collected 13.5 dpc were significantly higher in group A than in group F (27.5+/-14.0% versus 12.3+/-7.1%, and 1362+/-687 versus 615+/-353, P=0.001, respectively). The CD45+CD86+ percentage was around 7.5% in nonpregnant CBA/J mice, similar to the 10.6% in CBA/JxDBA/2 mice 5.5 dpc, but had increased dramatically, to 23.9%, by 9.5 dpc (P<0.001 versus nonpregnant mice and P=0.002 versus CBA/JxDBA/2 mice 5.5 dpc), and remained at a higher level (27.5%) until 13.5 dpc. However, this trend was not observed in group F during pregnancy. The increased CD45+CD86+ percentage at day 9.5 of gestation, when resorption begins, may support the assumption that CD45+CD86+ cells play a role in the course of embryo resorption. Lymphocyte phenotypic analysis in the lymph nodes that drain the pregnant uterus may be helpful to assess the status of local immunity at the feto-maternal interface.Journal of Reproductive Immunology 12/2004; 64(1-2):133-43. DOI:10.1016/j.jri.2004.08.002 · 2.37 Impact Factor
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The food is a usual Thai food, but the service is great and the restaurant is so clean and well-decorated.
I had fried rice with beef and the quality of meet was above the other Thai restaurants in the area.
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text:
I am adding a custom data attribute
data-js-href to various HTML elements, and these elements should behave just like a link when clicked. If a link within such an element is clicked, the link should take precedence and the
data-js-href functionality should be ignored, though. Furthermore, the solution also needs to work with elements that are dynamically added at a later time.
So far, I have come up with the following solution. It basically checks if the click was performed on a link, or any child element of a link (think
).
// Make all elements with a `data-js-href` attribute clickable
function(event, clicked) {
var link = clicked.get('data-js-href');
if (link && !event.target.match('a')) {
var parents = event.target.getParents();
for (var i = 0; i < parents.length && parents[i] != clicked; i++) {
if (parents[i].match('a')) {
document.location.href = link;
It works, but it feels very clumsy, and I think that there has to be a more elegant solution. I tried something along the lines of
$$('body').addEvent('click:relay([data-js-href] a)',
function(event, clicked) {
but to no avail. (I littered the code with some
console.log() messages to verify the behavior.) Any idea is welcome.


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Tropea - Calabria, Italy - I stayed between Tropea and Capo Vaticano. Tropea is a lovely old town sitting on a hilltop with narrow winding streets. Below the town is a long stretch of golden beaches. Capo Vaticano is further down the coast and has spectacular scenery, golden beaches and emerald green seas - more of a resort area with many hotels, bars, restaurants etc.
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Scilla, Calabria, Italy I'm going to Italy in 2013...hope this is on my itinerary!
Scilla, Calabria, Italy. Scilla is a town and comune in Calabria, Italy, administratively part of the Province of Reggio Calabria. It is the traditional site of the sea monster Scylla of Greek mythology. Wikipedia
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text: Zhorde Net Zombie survival | minecraft servers, Find the best minecraft servers with our multiplayer server list. browse detailed information on each server and vote for your favourite.. Original minigame - minecraft, About kitchen sink: kitchen sink is a silly and fast-paced new minecraft minigame. players can pick up everything around them and throw it at their opponents to do. References: minecraftservers.org www.planetminecraft.com


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When just 5 years old, Odette made her first appearance in a hollywood cast, playing one of the incredibly cute kids in the fun movie Kindergarten Cop. Obviously a memorable experience, because all through school Odette took an enthusiastic interest in drama. On leaving her high school, Woodcrest Christian, Odette started out on a career in modeling.
In 2006 Odette got her break into TV with a lead role in the series South Beach. Both Odette (playing a sexy model) and the location were extremely glamorous, but this wasn't enough to save a tedious show, which folded after just 8 episodes.
Odette stayed with TV drama for her next part, appearing in both series 1 and 2 of October Road, a show completely trounced by nearly all the critics. The story centers around a writer returning to his hometown to reconnect with his old friends, whose characters he had stolen for his best-selling book. Odette played Aubrey, the love interest for the leading man. Again the show may have bombed, but Odette had gained valuable experience and exposure.
Next Odette landed a big part in the the movie Cloverfield, a J.J. Abrams produced blockbuster about a monster rampaging through New York. Despite a huge promotional campaign of online hype, the movie opened to mixed reviews and reactions. Athough the 'first-person' camera perspective was intended to add immediacy to the action, it only succeeded in making many in the audience feel sick.
2009 saw the opening of The Unborn, a horror movie in which a woman fights against demonic possession by her unborn twin. Odette contributed both a great performance, and a lovely silhouette on the movie's promo poster.


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In the early days of their courtship, Harold told Antonia, "I am loopy about you: I feel 18."
It was pretty much as loopy and over the top as an episode of the show.
With Reiner playing the straight man, Caesar plays a wise-cracking and loopy archaeology professor.
Crazy; silly; nutty: that loopy guy whose handkerchief you cry into/ visually complemented the singer's loopy Balkan bop/ even loopier bids for the few works in Wood's small mature oeuvre (1925+)


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Dan Catchpole | email@example.com
Published: Monday, July 29, 2013, 10:01 a.m.
Boeing 787-9 leaves paint hangar with new livery
The Boeing Co. has painted the first 787-9 in a new company livery, though it's not a radical departure from the old one. The new plane was rolled out of the paint hangar at Paine Field last week. The company plans to fly the plane later this summer and deliver the first 787-9 in the middle of next year. Here's the news release:
EVERETT, Wash., July 27, 2013 /PRNewswire/ -- Boeing [NYSE: BA] continues to make progress on the first 787-9 Dreamliner, which also has become the first 787 to don the new Boeing Commercial Airplanes livery. The airplane has just rolled out of the paint hangar.
This refreshed look for the Boeing family began with the 747-8 and evolved with the 737 MAX. The new livery retains many of the features of the original 787-8 livery, adding a prominent number on the tail to help distinguish among models within the same product family.
The 787-9 will complement and extend the 787 family, offering airlines the ability to grow routes opened with the 787-8. With the fuselage stretched by 20 feet (6 meters), the 787-9 will carry 40 more passengers an additional 300 nautical miles (555 kilometers), with 20 percent less fuel use and 20 percent fewer emissions than similarly sized airplanes. The 787-9 leverages the visionary design of the 787-8, offering the features passengers prefer such as large, dimmable windows, large stow bins, modern LED lighting, higher humidity, a lower cabin altitude, cleaner air and a smoother ride.
Boeing is on track to roll out and fly the 787-9, currently in final production, in late summer. First delivery to launch customer Air New Zealand is set for mid-2014.
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Hexham Enthusiast
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- Good fit. Stylish. Keeps you nicely warm without being bulky.
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Published on: 04 June 2015
Womens Strata Vest
Warmth, weather protection and fantastic breathability, all in a superbly light and packable bundle.
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This extra breathability makes the Strata Vest ideal for more energetic activities, making it the perfect complement to the slim, sporty, feminine cut of this extremely useful gilet. It also allows the Strata to work really well as a midlayer, but the wind and water resistant fabric means it's great when worn on its own too.
Stuff it away into its inner pocket and you've got a tiny little package - weighing less than a can of pop - to stow away in your pack. Chances are you won't want to do that though: you won't want to take it off!
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text: I'm sure it's been asked before, so I'll ask again. For first time BW film developing, can someone recommend some film and chemicals? I'm looking for something affordable, easy to work with, and fairly forgiving -- unless they all work about the same. I'll probably take my first roll through my pinhole camera. I've been using 400 speed color film and getting it professionally developed. Thanks to Bill Barber, I have some supplies to work with. I just need film and chemicals.


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Melanie Griffith Is Probably Regretting That TattooBy: Toni Matthews-El - July 14, 2014
Melanie Griffith has learned a hard lesson about tattoos: Unless you can guarantee you'll be with him or her forever, it's best to leave your lover's name OFF of your body!
The 56-year-old actress recently parted ways with Antonio Banderas. Griffith was married to the 53-year-old actor for eighteen years. Unfortunately after nearly two decades together, the romance went south.
The couple released a joint statement regarding their amicable split in June:
We have thoughtfully and consensually decided to finalize our almost twenty years marriage in a loving and friendly manner honoring and respecting each other, our family and friends and the beautiful time we have spent together
Although Griffith may not be publicly bitter about the divorce, she seems to be very much over her former flame. The actress has engaged in a series of tattoo removal procedures to be rid of her "Antonio" tattoo.
A recent photo of the actress showed her out and about with a bandage over the tattoo.
It was also apparent that her former beau's name was getting lighter and lighter.
As of now the name "Antonio" is no longer visible, though it appears that Griffith might keep the rest of the heart-shaped tattoo.
Could Griffith be making the space available for a future love interest? Possibly.
However if the actress is wise, she'll forget about a new name on her arm to signal a new romance.
Many tattoo-happy individuals will forgo tattooing anyone but "Mom" on them as the decision is actually seen as a kiss of death, almost guaranteeing the relationship will end in a separation. And when you DO break up, it can be especially crappy to see a constant reminder of lost love every time you look in the mirror.
The removal process may have been painful, but no doubt Griffith is better able to enjoy single life without her ex's name on her arm.
Image via YouTube


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"Sunstone" Recovered From Sixteenth-Century British Ship
Wednesday, March 06, 2013
ALDERNEY, CHANNEL ISLANDS—Tests indicate that a piece of carved crystal recovered from a sixteenth-century shipwreck at the bottom of the English Channel is calcite. Calcite crystals have been shown to reveal patterns of polarized light around the sun, and could have helped early navigators determine its position in the sky on cloudy days. This crystal was found near the ship's navigation equipment and may have been used as such an optical compass. These crystals are often found in Iceland and Scandinavia, and may have been the "sunstones" of Norse legends. This is the first time that archaeological evidence has been found to support the "sunstone" interpretation.
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By Norm Schrager on Tuesday 1st November 2005
But he has the guts to play a version of himself in this smart comedy, one in which Kornbluth is in every single scene, riding the surprisingly lively script into the world of office temp stardom.
And that screenplay is very close to Kornbluth's heart -- written by him and his brother Jacob (they're co-directors and co-producers, as well), it's based on a monologue of Josh's, chronicling his life as a temp in San Francisco, and the horrors that can occur when permanent employee status comes knocking at your formerly comfortable career door.
In fact, the story is so close to the real Josh that he opens the film with a charming disclaimer, saying that actors were used in the film and that no "real lawyers" were portrayed (much of the action takes place at law firms). To further state his point, he tries to convince us that the setting is the beautiful, fictional town of "San Franclisco."
On paper, the comparisons to Woody Allen are easy -- a well-weaved combination of both whip-smart comments and broad comedy -- but those disappear once you see Haiku Tunnel. It's got a more agreeable simplicity than Allen's movies, both in its presentation and in its characters. Josh easily explains, with no apologies, that being a temp gave him such a simple pride, and he was never late. However, the intricacies of being a permanent staffer (but having the same job tasks!) make him late all the time. It's got a certain sweetness.
And that succeeds through most of the film, even when the story gets a bit surreal for the sake of fun. Josh's minimal success, his sad, fellow secretaries in the firm and the female lawyer that begins to fall for him all have a real human side that the brothers/directors make sure to bring out. It injects a film that often looks like a first feature with a cool dose of humor and maturity.
Comedies about the workplace have certainly been done before, and recent movies like Office Space and Clockwatchers have collected loyal fans by expressing the boredom and pain of today's 9-to-5 in a cube. By reducing the experiences to one man -- one, funny, neurotic, sloppy man -- we get a different take, with a guy that can write, direct, produce, and truly star in his own feature. Sounds like a good job.
The DVD has a number of extras, including some outtakes and deleted scenes worth a chuckle. The commentary by both Josh and his co-director brother Jacob is also quite amusing -- like hanging out at a bar with the two for a couple of hours.
Reviewed as part of our 2001 Boston Film Festival coverage.
File it.
Year: 2001
Run time: 88 mins
In Theaters: Monday 22nd January 2001
Box Office Worldwide: $251.1 thousand
Distributed by: Sony Pictures Classics
Contactmusic.com: 3.5 / 5
Rotten Tomatoes: 60%
Fresh: 28 Rotten: 19
Director: Jacob Kornbluth, Josh Kornbluth
Producer: Brian Benson, Jacob Kornbluth, Josh Kornbluth
Screenwriter: Josh Kornbluth, Jacob Kornbluth, John Bellucci
Starring: Josh Kornbluth as Josh Kornbluth, Keith Warren as Bob 'Bob' Shelby, Sarah Overman as Julie Faustino, Helen Shumaker as Marlina D'Amore, Amy Resnick as Mindy, Brian Thorstenson as Clifford, Joanne Evangelista as Caryl, Joe Bellan as Jimmy the Mail Clerk, Michael X. Sommers as Crack Attorney, Jodean Lawrence as Denise the Receptionist
Also starring: Warren Keith, Brian Benson, Jacob Kornbluth, John Bellucci


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Just a regular mob beating. Phew!
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Spokesman Josh Earnest blamed Republicans for blocking the president's efforts to catch criminals.
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Size Chart
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Did my 4th tri and I love this suit. There are a lot of them out there and making a decision can be challenging. I chose this one from a features and price point and it is excellent. Transition times were excellent and I also swim in my pool with it. I just wash with wet suit wash that eliminates chlorine each swim. Great fit. Thank you.
Update the wetsuit diagram faq custom merge code to change this.


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£1,250 pcm
4 bedroom detached house to rent Suckling Green Lane,Codsall,Wolverhampton,WV8Superbly presented extend and improved attractive four bedroom dormer residence providing accommodation of much style and character in a sought after location More details ›
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3 bedroom semi-detached house to rent MOUNT GARDENS, CODSALL, Wolverhampton, WV8A three bedroom semi detached property situated in a central village location, convenient for local shops and amenities. Accommodation comprises; Entrance Hall, Lounge and Dining area off, fitted Kitchen, three Bedrooms and Bathroom, Garage and gardens. AVAILABLE MID AUGUST. More details ›
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4 bedroom detached house to rent Lansdowne Avenue, Codsall, Wolverhampton, South Staffordshire, WV8A well presented and well proportioned, modern family house standing in a sought after, tree-lined Avenue in a popular South Staffordshire village. Boot room, reception hall, cloakroom, lounge, dining room, conservatory, kitchen, laundry, useful lobby and workshop / study, four double bedrooms, ... More details ›
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3 bedroom semi-detached house to rent Brook Grove, Codsall, WolverhamptonModern style semi detached home in cul-de-sac location. Neutral decor, gas c/h, d/g, gardens front and rear, modern style kitchen and replacement bathroom with electric shower. Available early July Unfurnished. More details ›
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The courage & strength are just a couple reasons Rachel is my warrior goddess… She had set out to reach her goals and went far beyond them.
This winter she entered her first competition & she powered thru a chilly shoot in the snow with me just a couple days later. Her body was frozen and still a bit dehydrated from preparing her comp body.. but man does she rock those shorts! What a hottie.. love you Rach, I'm so very proud of you!!


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Canine Chat: Dog Tales and Other Stories
The subject of dogs facilitates heartwarming pet stories, pet peeves and pet praises. Let's talk dogs.
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Are you Buying a Halloween Costume for your Pet?
Chase as an Avenger, kind of. c Diane Rich 2013 The retail industry is more than ready for the costume frenzy for those pet parents who love to search for that one special costume they feel will enhance their pet's personality. Costumes are no longer inexpensive and people are willing to open their wallets, suit up fido […] [Read More]
Book Review: What the Dog Knows
The Science and Wonder of Working Dogs by Cat Warren c Diane Rich 2013 Cat Warren brought her young GSD, Solo to a trainer who asked Cat an important question at the first class. The question was, "What would you like the dog to do?" The question wasn't meant for Cat to think short term as the […] [Read More]
The Color Purple has Gone to the Dogs
c Diane Rich 2013 Do you love purple? Purple-loving pet owners have several new reasons to howl for happiness, purple pet clothes, purple leashes and purple bowls! They even feature a purple pooper scooper. I was contacted by a representative from The Purple Store requesting I tell my readers about the newest additions to its pet product […] [Read More]
10 Halloween Safety Tips for Dogs
c Diane Rich 2013 1. Trick or Treat Halloween candy attracts both humans and dogs. Most owners know that chocolate, especially dark or baker's chocolate is unsafe for dogs, but so is almost everything else that you'd find in a trick-or-treat bag. Artificial sweeteners such as xylitol, raisins and other candy items can pose serious […] [Read More]
October is Adopt a Shelter Dog Month
Adopt a Shelter Dog Month promotes pet adoption and raises awareness about the millions of dogs currently living in shelters. It is estimated that about 50% of dogs in shelters are destroyed simply because there is nobody to adopt them. Adopting from a shelter is an alternative to buying from a pet store. Most people are aware that […] [Read More]
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Date: 1953 ca.
Description: Captain John Cotton residence. Also known as "Beaupre", this Greek Revival style house was built in 1842 for Cotton, a retired U.S. Army officer.
Date: 1905 ca.
Description: Exterior of Brown County Court House.
Date: 1900 ca.
Description: View from across street of the Whitney House. A man stands in the yard behind the fence. There are large trees along the curb, and more trees and shrubs in...
Date: 1873 ca.
Description: At the De Pere depot, the first train operated by the Milwaukee & Northern Railway. This train made its first run to Green Bay on June 25, 1873, and this p...
Date: 1920 ca.
Description: Aerial view of Green Bay shoreline.
Description: Hoberg Paper and Fibre Company buildings along the East River.
Date: 1906 ca.
Description: Exterior of St. Patrick's Church.
Date: 1950 ca.
Description: Air view of the Fox River, which is near Green Bay.
Date: 1961-12-29 ca.
Description: View from across street of the window display for Newman's "The Store of Fashions", which featured numerous "Titletown U.S.A." signs. The Packers were abou...
Date: 1963-10-13
Description: Gasoline station, with Standard Oil Products and Champion signs, in old false front building.
Date: 1930 ca.
Description: Close-up view of the cottage when it was a library. A sign is partly visible on the building stating, "Kellogg Public Library, Union Park Branch." Used as...
Date: date unknown
Description: View of a log house located at the top of Collard's Hill.
Description: View of Deaconess Hospital and Nurses' Home.
Date: 1910
Description: Interior view of the Green Bay Public Library. The library was built in 1903 at a cost of $12,000 by H.A. Foeller, Architects. Note on photograph reads: "I...
Date: 1995 ca.
Description: Green Bay Packer player (possibly Bob Kuberski) sitting on a young boy's bicycle and signing a notebook in the parking lot of Lambeau Field.
Date: 1973-01 before
Description: A wall calendar featuring the "World Famous Green Bay Packers." The calendar is advertising Hank Lefebvre's commercial photography business.
Date: 1955-10-08
Description: Adlai Stevenson is seated in a room with several other men.
Description: Formal portrait of F.W. Kellog.
Date: 1935
Description: This map is a photocopy and shows landownership and acreages, additions to Green Bay, points of interest, roads, and railroads. Hand annotations of green,...
Date: 1897 ca.
Description: De Pere lock on the Fox River with a construction crew at work. This is the only wooden lock on the Fox River.
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VIEWS: 28 PAGES: 6 CATEGORY: Childrens Literature POSTED ON: 12/16/2009 Public Domain
CHAIRPERSON'S ANNUAL REPORT 1998 - 1999 INTRODUCTION 1998 was a year of dramatic moments. Not only did the commission have to deal with several instances where complainants were not prepared to waiver their rights to take civil action in the courts against the broadcaster, but some matters were also quite sensational. Dr Louis Luyt filed two complaints against the SABC for biased reporting; a previous member of BOSS complained that he was implicated by the SABC in the training of gangs in the Cape; and Ferdie Barnard, who was later in the year found guilty of the murder of Dr David Webster, complained that he had been treated unfairly by the SABC. Other complaints concerning alleged obscene jokes on early morning radio, the taking in vain of the Lord's name in TV sitcoms and feature films were reaching the Commission weekly. In two instances the question arose as to whether the Commission could decide upon a matter while a High Court case was pending and our finding might have a bearing on the merits of that case. THE WAIVER CLAUSE The Rules of Procedure of the BCCSA provide that where the Chair is of the opinion that it is in the interest of fairness that a complainant waive his or her rights to take civil action in the courts on the same matter, the Chair shall require the complainant to waive his or her rights. Naturally complainants have not always been happy with this requirement. In the case where Mr Slang Van Zyl complained that he had been implicated unjustifiably by the SABC in the training of Cape gangs, he was not prepared to sign the waiver clause. The Chair motivated his requirement by explaining that the elements of this complaint were almost identical to those which formed part of a claim based on defamation and that it would be unfair to ask the SABC to answer the same case twice. On the other hand, Mr Ferdie Barnard who complained that the SABC had not given him a fair opportunity to answer certain allegations made against him about the murder of Dr David Webster, was prepared to accept the waiver. When Mrs Elna Boesak, wife of Dr Allan Boesak, who was one of the accused in a prosecution in the Cape, complained about omissions by the SABC in its coverage of the case, she was also asked to sign a waiver. She objected and her objection was sustained on the grounds that a waiver by her would be meaningless since, if Dr Boesak himself were to sue, a waiver by 1 her would be irrelevant. I shall return to this matter in the paragraph which deals with court reporting. Dr Loius Luyt, then Chair of the South African Rugby Football Union, complained that the SABC had unjustifiably and incorrectly stated that he was in control of Ellis Park. He was willing to sign the waiver if a proper apology was broadcast. The apology was broadcast, but in a further programme, an interview with a financial journalist, the accusations were made once again. Dr Luyt filed a further complaint. When he was asked whether he would be prepared to sign a waiver, he said that he would Rather pursue the matter in the courts. The waiver clause is controversial, is therefore abundantly clear. On the other hand, the Constitution of the Republic guarantees that a person has a right to take a matter to court or, where appropriate, to have a matter settled by an alternative tribunal or forum. The BCCSA is such a tribunal and it is felt that it would be unfair to broadcasters to allow them to be put through similar procedures twice. As appears from the Boesak case, such a waiver is not necessarily required. In many cases the waiver clause is not even discussed. The matter only becomes relevant when the procedure before the BCCSA has substantially the same elements as those which a procedure before a court would have. COMMENTS ON CASES WHICH ARE BEING HEARD IN THE HIGH COURT It has often been said that reportage of the proceedings of our courts requires experienced journalists. The matter could become quite technical and involved and when the evidence from the State is led, the witnesses are cross-examined, the defence leads its witnesses who are once again cross-examined, it becomes quite difficult for a journalist to sum up that case in such a way that viewers get a true and balanced report. Mrs Elna Boesak felt that much publicity was given to the charges against her husband but that the subsequent cross-examination of many of those witnesses was not reported. The SABC's case was that once the defence calls its witnesses, they would be the focus of reportage. The BCCSA held that it was impossible at that stage to decide whether the matters were dealt with in a balanced manner or not Save in exceptional circumstances, the BCCSA would have to wait until the whole matter was concluded and then only would it be in a position to deal with a complaint on balance. On the other hand a statement made in an SABC broadcast on the High Court challenge by a Christian society on the constitutional validity of the latest legislation on abortion, and which gives choice to the mother if certain conditions are met - was adjudicated by the BCCSA. The statement was that the legal team which challenged the legislation consisted of men, whilst women defended the right to "choice" in the legislation. Tested against the facts, this was not true. Allowing some leeway for sensationalism, the BCCSA held that the SABC's statement was substantially correct. Viewers of that news programme would fall within a category which would know that the gender of a particular team is entirely irrelevant to a judge. It was accordingly held that the omission was not substantial and therefore did not amount to a contravention of the Code. 2 "APARTHEID DID NOT DIE" The broadcast of the documentary "Apartheid Did Not Die" on the SABC, led to some controversy. The theme of the programme was that, in spite of the new dispensation, "apartheid" between the rich and poor had now substituted the previous dispensation where the segregation had been between Black and White people. The complainant felt that the programme was not balanced insofar as interviews were not conducted with a representative group and that the research had not been done in a satisfactory manner. After the hearing, complaints were received from the producer, John Pilger, who said he had not been afforded the opportunity of putting his case. The Commission pointed out to him that the SABC was subject to the jurisdiction of the BCCSA and that only the SABC could decide whether it would call him to testify. Pilger complained that the hearing was reminiscent of a Franz Kafka novel "The Trial", and that a finding against the programme would prove that an injustice had been committed against him. As matters turned out, the Commission had at that stage already decided that, in the light of freedom of expression and debate, the likely audience would have had the necessary insight to know which opinions in the programme were well founded and which were not. The complaint was therefore not justified. The complaint that the panel, which discussed the programme afterwards, consisted only of men, was also rejected by the Commission. Although mixed gender representation is preferable, it was felt that it would be wrong to work with a fixed formula. The question was not who was on the panel but what was said. In the result is appeared that the Commission did not regard the panel discussion as necessary and that it would have come to the same decision even without subsequent discussion. This judgment can be regarded as an important milestone in the "freedom of speech" thinking of the Commission. RUDE JOKES AND PROGRAMMES INDECENT LANGUAGE IN EARLY MORNING The Commission has received numerous complaints from parents concerning rude jokes and indecent or obscene language during early morning programmes on radio. The Commission has taken into consideration that some of these programmes are directed at open minded people who would find this trend to be in accord with an open and democratic society. On the other hand it is true that some children might be exposed to this kind of language and to rude jokes. The Commission has decided to strike a balance between these conflicting interests. Where there is a blatant disregard of good taste, or where there is degradation, a finding of indecency or obscenity will follow. The matter is, however, far from resolved and further evidence, argument and discussion could possibly lead to more certainty. The fact that many broadcasting authorities have a similar problem illustrates that a simple solution will not bring the matter to a close. To follow procedure which has been followed by the US Supreme Court, to ban certain words, could also seem to be absolutist and not in conformity with the freedom of speech 3 guarantee and the rules of natural justice, which are required by the Constitution of the Republic. THE EFFECT OF THE CONSTITUTION ON THE CODE OF THE COMMISSION Since 4 February 1997, when the new Constitution came into operation, it has become clear that the Code of the Commission can only be interpreted properly if the Bill of Rights is taken into consideration. This because the new Constitution is also substantially horizontal in its application. This means that whilst the interim Constitution concentrated on the relationship between State and citizen, the present Constitution, where appropriate, also draws society itself into the Bill of Rights. This Commission has no doubt that whilst its Code limits the freedom of expression guaranteed in the Bill of Rights, it should function within the spirit of the Constitution. In fact, section 39(2) of the Constitution provides that when interpreting any legislation, and when developing the common law or customary law, every court, tribunal forum must promote the spirit, purpose and objects of the Bill of Rights. Since the Constitution to a great extent protects the rights of individuals, the Commission has been particularly sensitive in regard to these rights. THE WATERSHED On the other hand, the Constitution also protects the rights of children and special steps have been introduced by the Commission to guarantee this protection. This has been especially so in the case of feature films Publicity, timing and classification have become the foundations of broadcasting control in films. We have discussed this matter in depth with SABS-TV and M-Net and they have complied with our requests in this regard. The public should continuously be kept informed. It should also be conveyed to them that after 21:00 more explicit matter may be shown but, once again with due publicity, age restriction, classification and warning. We have also taken note of the parental mechanism supplied by M-Net and that parents, therefore, have control of age-restricted television films. We hope that the V-Chip for public broadcasting will eventually also be introduced in South Africa. We are aware of problems in this regard, but the concept is a good one and should be supported by the broadcasting industry. THE WORLD BROADCASTING SUMMIT FOR CHILDREN The Chairperson and the Alternate Chairperson, Ms Lauren Jacobsen, attended the World Broadcast Summit for children in London during March 1998. Much was learnt in this regard and contacts have been established with researchers in this field. Our broadcasters were well represented at this conference and, we believe, have brought new ideas home. The need for greater contact within Africa surfaced at this conference and a conference for Africa regulators should be organized as a matter of urgency in the near future. 4 MEMBERS OF THE COMMISSION During 1998 four new commissioners joined the Commission. They are Ms Melani Chait, who has wide experience of broadcasting and broadcasting codes; Prof Sunette Lotter, who is an expert on law and morality; Ms Windsor Leroke, who has wide experience in sociology and the needs of listeners and viewers; and Adv Lindi Nkosi-Thoma, who has substantial experience in the conducting of hearings within the rules of natural justice. Mr Justice Trengove and Ms Pearl Mashabela assisted the Chair in these appointments. The Commission also appointed Mrs Shouneez Martin as the new Registrar. Mrs Martin has thorough experience in the administration of the BCCSA and was sits secretary for four years. Ms Angelique Hargreaves has been appointed secretary. The Chair's secretary in Pretoria was Mrs A Rosslee. Mr Yolandie de Waard acted as a research assistant. Mr Alwyn Schutte, the accountant, has managed our budget. THE ROLE OF COMMISSION We are often asked whether the Commission has a direct influence on the content of broadcasts. A further question is whether some form of censorship could be exercised in regard to broadcasts. The answer to this is that the BCCSA is not authorized to act on any complaint before a broadcast and is also not authorized to initiate a complaint itself. Only after a complaint has been received and the complaint makes out a case that a broadcast has contravened the Code of the Commission may the complaint be adjudicated by one of the commissioners or, in more serious cases, by a panel of the Commission. The panel represents both listener and viewer interests and the interests of the broadcasters. The fourteen members of the Commission were appointed by an independent panel chaired by Mr Justice Trengove. The Commission, although funded by the member broadcasters, is entirely independent from them and the National Association of Broadcasters. This independence has been obtained by way of a contract according to which the National Association of Broadcasters is obliged to pay the reasonable costs of the Commission. The Commission has experienced excellent co-operation from its broadcasters and all hearing and adjudication's have been conducted in a spirit of "coproduction". Ultimately, in the last five years, having dealt with more that 130 complaints by way of full panel hearings, adjudicated or mediated more than 250 complaints and answered more than 4 000 complaints, we have come to the conclusion that indirectly we have influenced broadcasters in relation to balanced programmes, publicity, classification of warnings and a realisation of sensitivities. On the other hand, within a "freedom of expression" era, it would be unacceptable for this Commission to adopt a hard line. Therefore it has become characteristic of this Commission that prefers the "light touch", 5 where the aim is rather to inspire its broadcasters to greater quality than to subject them to rigorous control. Kobus van Rooyen S.C. Chairperson 6
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The Department of Business Administration in the College of Business at the University of Illinois brings together multiple business disciplines to nurture a diverse and supportive learning community. We're researchers. We're educators. And we believe in the power of higher education. Looking to find your place in the world of business? We'll show you how.
- Why Business Schools Want More H-1B Visas
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Maker Certificate Featured on WCIA
The Illinois MakerLab's new certificate was featured on WCIA's nightly news. Read the feature and visit the MakerLab website.


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|Knights Armament
|Part Number:
|Long Rifles - Rifles
Knight's Armament SR-M110 Rifle System in 7.62mm with a 20in barrel in tan.
The Knights Armament 25640-1 ships standard with the following: SR-M110 Rifle, complete deployment package
We are not currently accepting back orders for this item. Please add the SR-M110 in 7.62x39, Knights Armament part number 25640-1 to your watch list to be notified when this item is available again.
In order to be competitive, our prices reflect a 3% discount for payment by cash, check, or money order. Other forms of payment will be subject to an additional $456.79 in processing fees. Arizona residents are subject to 8.3% sales tax.
When purchasing a firearm or other restricted item, federal law requires that it MUST ship to a licensed dealer in your state. If you need assistance locating a dealer to have your firearm shipped to, please contact us or enter your zip code at the GunBroker FFL dealer network website.
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Custom Recipes for
Greek Veggie Salad II
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|patt s.
|Sent on:
|Sunday, March 14, 2010 3:17 PM
Sounds like fun. Count me in. I will bring a pepperoni loaf.
(kind of like a sub, but with pepperoni)
----- Original Message -----
From: "Ed O." <[address removed]>
To: [address removed]
Sent: Thursday, March 11,[masked]:04:55 PM GMT -05:00 US/Canada Eastern
Subject: [boardgames-654] New Meetup: Board Game Potluck (Antioch)
Announcing a new Meetup for Board Game Lovers Meetup Group!
What: Board Game Potluck (Antioch)
When: Saturday, March 20,[masked]:30 PM
June's Place
Will email to RSVPs closer to the event
Nashville, TN 37201
The last potluck was so much fun that we need to do another one. And June has graciously allowed us to use her new place. (Which is a "no-shoes-indoors" location). So please bring some food and include which dish you are bringing in your RSVP. I will be bringing some turkey wraps. I will email folks the address after they RSVP a few days before the event.
As usual I will bring a wide selection of games. Feel free to bring any games you would be willing to teach to others. And remember please make your RSVP count and update your RSVP if your plans change.
Parking: you can park in the spaces marked "visitor" near the mail boxes.
See you there.
Learn more here:
Please Note: If you hit "REPLY", your message will be sent to everyone on this mailing list ([address removed])
This message was sent by Ed O. ([address removed]) from Board Game Lovers Meetup Group.
To learn more about Ed O., visit his/her member profile
To unsubscribe or to update your mailing list settings, click here
Meetup, PO Box 4668 #37895 New York, New York[masked] | [address removed]
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Tempo Wrist-Worn Health Tracking Device for the Elderly
Published by Sierra Monica B. on August 14, 2014 under Workout
Here is a new health tracking device that you can wear on the wrist, which was designed for the elderly. Developed by CarePredict, Tempo has built-in sensors in charge with monitoring the user's sleep quality and the amount of sleep, eating, bathing, cooking, and drinking. Read more
Personalized Chalkboard
Published by Sierra Monica B. on August 14, 2014 under Decor
The Message Board is a magnetic chalkboard that you can customize to meet your needs in public places like bars and restaurants or at home. You can write messages on it, draw and even add photos by sticking them to the board using cork magnets which are handmade from recycled cork. Read more
Always in View Baby Monitor for Cars
Published by Sierra Monica B. on August 12, 2014 under Outdoors
Always in View is a new wireless baby monitor developed by Infanttech for use in cars. The main function is to allow parents to monitor their babies, without having to turn around and get distracted from driving. Read more
iStabilizer Monopod and Shutter Remote Bundle
Published by Sierra Monica B. on August 12, 2014 under Mobile
iStabilizer announced the monopod and shutter remote bundle for those used to take selfies everywhere. Read more
EasyBulb Plus Light Bulb Controlled with the Smartphone
Published by Sierra Monica B. on August 11, 2014 under Home
EasyBulb Plus, developed by Gigateq Limited, is a new light bulb with smart features, which allows you to control light in your home or office with the smartphone. The dedicated application installs on Android, iOS and soon on Windows phones, and allows you to change the light color to match it with your current mood, control several lights at a time, or set the lights to dim gradually when it's time to sleep. Read more
Yantouch Diamond+ Bluetooth Entertainment System
Published by Sierra Monica B. on August 3, 2014 under Fun
Diamond+ is a new Bluetooth speaker that stands out with advanced features which make it a great entertainment system, a music and light therapy device, and a sleep aid device. Developed by Yantouch, the speaker synchronizes with your music aource to produce amazing light shows from 16 million colors, synchronized with the beats. You can set the mode to match your mood or event, choosing from solid, saturation, bright, flashing, alternating lights, and others. Read more
SETA Universal Stand
Published by Sierra Monica B. on July 31, 2014 under Mobile
"L"-shaped stands are not something new, but I like how this model inspires simplicity and ease of use. SETA was created to hold tablets and smartphones without occupying much space on a desk or adding bulk to devices it holds. Read more
Zcan Wireless Mouse and Portable Scanner
Published by Sierra Monica B. on July 29, 2014 under Mobile
DTOI (Design to Innovation) launched the wireless mouse and portable scanner called Zcan Wireless. Shaped like a regular computer mouse, Zcan connects to your desktop, laptop or tablet via Bluetooth and operates like any other mouse. The second function is scanning. You can use the mouse to scan documents and photos anywhere you are and have them instantly transferred on the computer. Read more
Kisai Kaidoku Wood Displays Time with Words
Published by Sierra Monica B. on July 29, 2014 under Mobile
I'm happy to see that Tokyoflash Japan launched a LED watch that displays time using words instead of signs. This makes it a lot easier to read the time if you're not used with Japanese watches from Tokyoflash and their unusual system of displaying time. Read more
Win the Loop, the Earbud Anti-tangling Device!
Published by Sierra Monica B. on July 23, 2014 under Giveaways
The Loop is a new anti-tangling and storage device launched at the beginning of this year at the 2014 Customer Electronics Show in Las Vegas, which keeps your earbuds organized and tangle-free, so you can use them right away.
The audio accessory is small enough to fit inside your pocket or bag and comes in many colors that you can choose from. You can use it at the gym, mall or beach, in the bus, and all the places where you need to use earbuds. Read more


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Port Tampa Bay eyes direct routes to Panama Canal
One of Port Tampa Bay's goals for the coming year is to establish direct service to and from the Republic of Panama. Port CEO Paul Anderson brought that up at a media briefing Thursday in the wake of a Tampa contingent's recent trade mission to that Central American country.
Currently, PTB shipping routes require stops in transshipment hub ports in either Kingston, Jamaica, or the Caucedo, Dominican Republic.
That's because of the way the container shipping line industry has evolved over the last couple of decades, explained Greg Lovelace, the port's director of marketing and business development. Mega-ships carrying containers globally pull into a hub, where cargo is transferred to smaller feeder ships, which then embark to places like Tampa.
"It's very similar to the airports hub and spoke system," Lovelace said.
Panama is a hub port as well, and has the added allure of being near the Panama Canal.
Each market's applicable hubs are determined by the major shipping companies. "A market like Tampa Bay needs to justify making itself a call port," Lovelace said. "Is there an opportunity to fill ships up coming in and out? Is the market going to support it?"
With its 43-foot depth, PTB can accommodate 80 percent of the world's fleet, but not the latest mega-ships. Even if the port could handle such a vessel, though, it's doubtful a shipping line would schedule a route here, simply because the Tampa Bay market isn't big enough to support it.
But adding Panama as a transshipment hub has real value to the local port.
"The Panama Canal is the most strategic shipping lane in the Western Hemisphere," Anderson said in a statement today. "To have a direct connection between Panama and Port Tampa Bay will provide short transit times and competitive advantages for the importers and exporters in Central Florida we serve, as well as complement our existing transshipment ports."
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I am a 24 year old woman who has never worn a bikini. Ever.
I have a mental list of things that I want to do in life before I die. I'll admit that most of them are the standard "must-do's"– see certain parts of the world, read certain books, do a headstand, etc. but there is one that I always put on the bottom of my list as the least likely thing I'll ever carry out (least likely, even, than climbing Kilimanjaro or becoming a household name). That is: .
The problem is in the fact that I don't just want to wear a bikini. I want to look good great in a bikini.
So, here I am an insecure, perpetually chubby blogger with a body image complex and a lofty goal: to don a bikini by my 25th birthday. I have 6 months to get in shape physically and to get myself mentally prepared (yes, I know how stupid that sounds). I intend to use this blog to track my progress, but primarily to inform people of what I'm doing so that when July 27th rolls around I absolutely can't chicken out.


text: I visited this hotel the evening before going to the Hannover messe the next day. Lovely place with great food (it comes with a price but it was worth it) Nearby is another restaurant at the Schaumburg with a great view over the valley. Just walk through the old tower and have a look. The room was a single and... More


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