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Organizing the home is a challenge. Organizing toys is a challenge…combining both in a small transportable space ups the ante for car organization! Does your family *live* in the car during the warm months? It seems like summer is filled with places to go and things to do. Our car needs to keep up. "Give everything […]
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North Indian Lamb Chops
14 Sep 2011
|Plain yogurt
|1 3⁄4 Cup (28 tbs)
|Ground turmeric
|1⁄2 Teaspoon
|Ground coriander
|1 Teaspoon
|1⁄2 Teaspoon
|4 Clove (20 gm), peeled and finely chopped
|Vegetable oil
|1⁄2 Cup (8 tbs)
|Dried red chilies
|4 Small
|Cinnamon stick
|1 , broken up
|Black mustard seeds
|1 Tablespoon
|Lamb chops
|Chopped fresh coriander
|4 Tablespoon
Mix the yoghurt, turmeric, ground coriander, salt, and garlic together in a bowl and reserve.
Heat the oil in a large saucepan over medium-low heat, and when hot, add the chiles, pieces of cinnamon stick, and mustard seeds.
Let them cook for 4-5 minutes.
Add the lamb chops and cook them for 8-10 minutes, turning them halfway through.
Pour in the yoghurt sauce and continue cooking over low heat for 15-18 minutes, until the meat is just tender.
Sprinkle the chopped fresh coriander or parsley on top and serve with Saffron Pilaf and a simple raita made of beaten plain yoghurt.
Recipe Summary
Difficulty Level:Easy
Course:Main Dish
Preparation Time:10 Minutes
Cook Time:25 Minutes
Ready In:35 Minutes
Rate It
0 Comments
North Indian Lamb Chops Recipe

subset: commoncrawl


text:
Click here to print the obituary below
Stella H (Weiss) Wagner
Stella H.(Weiss) Wagner 94 of the Keystone Villa at Douglasville Pa, wife of Paul H
Wagner for 72 years died at home on Sunday February 24, 2013.
Born at home on the farm when her parents lived near Porter's Mill in South
Coventry Township, Chester County, she was the daughter of the late Morris and Ethel
(Hunsberger) Weiss.
Stella was a resident of Pottstown most of her life and attended Pottstown Schools. She was a homemaker and employed by Schuwall Dress Factory during her early adulthood.
Stella enjoyed collecting open salts and advertised match books. Active in several social
Clubs, she also loved crocheting, sewing, reading, watching TV and gardening. She enjoyed travel, especially day trips. She was an active member of Trinity Reformed Church of the U.C.C.
Stella was the mother of two daughters, Carol Lee, wife of Harry W. Loshnowsky Jr. and
Joanne Marie Teassdale who died on Jan. 4, 2009.
Also surviving are her grandchildren Trish Ann Bonn of Sandia Park, NM, William P
Teasdale of Roswell, NM, Margaret E. Teasdale of Stone Mountain, GA, Jill Lynn Strodoski, husband Charles, of Landisville, and Bradley D. Loshnowsky, wife Jennifer, of Amarillo, TX; and her great grandchildren Tarin Robin Bonn and Gareth Arkeigh of
Sandia Park, NM, William Caleb Teasdale of Roswell NM, Casey and Megan Strodoski of Landisville, and Savannah and Harrison Loshnowsky of Amarillo, TX. Stella is also survived by one niece Jane Fink wife of Paul (deceased).
Stella is preceded by her sisters, Kathryn (Will) Brown, died in Tolensville, Staten Island, NY. and Elizabeth ""Lizzie"" (Rissell) Keifrider died in Nov. 1990 in Boyertown. She is also preceded by niece Ruth (Rissell) Auman wife of Sterling also deceased and nephews Richard L Rissell and wife Delma (deceased), Robert P Will husband of Emily, Morris Rissell (deceased) husband of Betty.
Relatives and friends are invited to a viewing at Trinity Reformed Church of the U.C.C.
Hanover & King Streets Pottstown, PA from 10:00am to 11:00am on Thursday, Feb. 28 followed by a Service celebrating Stella's life. Burial will be at Highland Memorial. In lieu of flowers memorial contribution may be made to Trinity Reformed Church of the U.C.C. 60 N. Hanover St. Pottstown, PA. 19464 or any local Hospice Care.
Catagnus Funeral Home & Cremation Center, Inc., 711 N. Franklin Street, Pottstown is in charge of arrangements.
View obituaries or send condolences at
Funeral Home
Catagnus Funeral Home
711 North Franklin Street
Pottstown, PA 19464
Published in The Mercury on Feb. 26, 2013


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text:
Critical Components Inc
120 Interstate N Pkwy SE,
Vinings, GA 30339
Phone: 770-933-5511
Fax: 678-868-2211
5 - 9
500k - 1 Million
Services->Other Biz Services
SIC Code:
5063 - Electrical Apparatus and Equipment
Full company description is available with the free trial.
Additional Info:
Lead411 offers quality contact information on a variety of companies including that of Critical Components Inc's CEO . The company profile also contains facebook info, linkedin accounts, phone numbers, cfo contacts, and Critical Components Inc email addresses with the domain format of @criticalcomponents.net. Critical Components Inc, is in the Other biz services field. If you sign up for our free trial you will see our email@example.com addresses.
Company Background:
Full company description is available with the free trial.
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Company Directory > Critical Components Inc


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Movie Reviews
9:09 am
Sat August 10, 2002
Miramax PG-13
By John DeSando, WCBE's "It's Movie Time"
Men who have dated younger women may have difficulty with "Tadpole": it chronicles the sexual adventure of a 15 year old prep-school intellectual with his step-mother's 40 year-old best friend while he really wants his step mom. The difficulty I speak of is the certainty with which society would pursue any man with a 15 year-old girl, whereas in this film the reverse is just a curious escapade. Thank goodness for the recent "Lovely and Amazing," in which the older woman is charged with statutory rape.
Director Gary Winick's "Tadpole" is not as energetic as "The Graduate" or as funny as "Rushmore," but its charm comes from its low-key attitude toward transgressive sex without consequence. Oscar Grubman (Aaron Stanford) is no ambivalent Benjamin: he knows he wants his step mom and won't even brook for long the embarrassment of a brief affair with her best friend, Diane (seductively and sassily played by Bebe Neuwirth).
Oscar's precocious love of Voltaire, whom he quotes just a bit too often and his useful French are attractive to older women but plain unbelievable, I hope, to the mature audience. No one accused Lolita of being an intellectual.
The ending is not quite satisfying-I am suffering from ending despair these days after seeing the uncreative close to "Signs"-but then I forget almost all endings anyway. That it was filmed in digital video, with all the attendant graininess, orange, and shakes (looking like Steven Soderbergh's "Full Frontal") is more a nod to Dogma 95 than a pleasure to a visually-oriented audience.
Character development and inventiveness are important to any storytelling: "Tadpole's" short 77 minutes barely get Aaron developed, but the inventiveness of the situation is refreshingly there from the un "Graduate"-like party to a rare step-mom encounter. "Tadpole" adds weight to a growing pop-culture formula, taking the heat off the infamous January-May for men and sharing it with women.
John DeSando co-hosts WCBE's "It's Movie Time" and vice chairs the board of The Film Council of Greater Columbus.


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Visitor Calendars
The tours and programs calendar below shows current availability for all of our regularly scheduled tours. (Visit the Group Tours page for information on booking tours for groups of 9 or more.) Some tours and programs require reservations. Make or modify reservations using links located on the tours and programs calendar.
July 4th Weekend Closure
The Visitor Center will be closed and will not be offering campus tours July 3rd-5th in observation of the holiday. Self-guided tours maps can be found by clicking the "Self-Guided Tours" link in the footer of our website. We will resume campus tours on Monday, July 6th.
Student Forum Offerings
Student Forums will be available for prospective students periodically throughout the summer. Please check back on this announcements page closer to your visit date to see if Student Forum will be offered during your visit to campus. Currently, Student Forums are scheduled for July 6th and July 10th.
Tour & Program Registration
Discover Stanford programs, golf cart tours, and other Special Interest tours for the first half of 2015 (through September 30) are now available for online registration.
Humanities and Arts Tour
During the Spring 2015 quarter, Visitor Information Services began offering student-led "Humanities and Arts" tours. The tours last for one hour, and online pre-registration is required. The tour gives an overview of the humanities and arts at Stanford, and passes by a number of buildings on campus, including Bing Concert Hall, Anderson Collection, Cantor Arts Center, Rodin Sculpture Garden, Main Quad and Green Library. We will be adding our limited summer Humanities and Arts tours to the calendar soon.
Visitor Center hours
- Open Monday through Friday from 8:30 a.m. to 5:00 p.m. (PT)
- Open Saturday and Sunday from 10:00 a.m. to 5:00 p.m. (PT)
- Hours subject to change (refer to the announcements).
Operating calendar
You may also want to view our operating calendar for the current academic year (8/15/2014 - 8/13/2015), which indicates dates of operation and closure for the Visitor Center, all Visitor Center tours, the Hoover Tower Observation Platform, and the Discover Stanford program, as well as drop-in hours for the Office of Undergraduate Admission.
Tours and programs calendar
Use this calendar to check the schedule of walking tours and register for the Discover Stanford program, engineering quad tour, or golf cart tour.


text:
Economics Departments, Institutes and Research Centers in the World
Økonomisk institutt
Universitetet i OsloTranslation: Department of Economics
Translation: University of Oslo
Location: Oslo, Norway
Homepage: http://www.oekonomi.uio.no/
Phone: 22 85 51 27
Fax: 22 85 50 35
Postal: Pb 1095, Blindern, 0317 Oslo
Handle: RePEc:edi:souiono
Additional details:
Publications at this institution that are listed with RePEc:
People who have registered with RePEc and have claimed to be affiliated with this institution:Find all publications of all authors listed below conveniently compiled on IDEAS. Authors with publications are listed below with . We have lost contact with authors marked with ?, and they do not count towards the ranking of this institution.
- Asheim, Geir B.
- Barth, Erling
- Biorn, Erik
- Bjerkholt, Olav
- Bojer, Hilde
- Brinch, Christian N.
- Brekke, Kjell Arne
- Civcir, Irfan
- De Haan, Monique
- Diekert, Florian Klaus
- Førsund, Finn R.
- Framstad, Nils Chr.
- Harstad, Bard
- Havnes, Tarjei
- Hagedorn, Marcus
- Holden, Steinar
- Holter, Hans Aasnes
- Hoel, Michael Olaf
- Keilman, Nico
- Kotsadam, Andreas
- Kristjánsson, Arnaldur Sölvi
- Leuven, Edwin
- Lind, Jo Thori
- Lund, Diderik
- Mehlum, Halvor
- Mideksa, Torben K.
- Midttømme, Kristoffer
- Mitra, Anirban
- Müller, Andreas
- Moene, Karl Ove
- Moen, Espen R.
- Moxnes, Andreas
- Nesje, Frikk
- Nilssen, Tore
- Nævdal, Eric
- Nymoen, Ragnar
- Nyborg, Karine
- Ognedal, Tone
- Piacquadio, Paolo Giovanni
- Rodseth, Asbjorn
- Russo, Alessia
- Spiro, Daniel
- Sørensen, Erik Ø.
- Storesletten, Kjetil
- Sun, Gang
- Thoresen, Thor O.
- Torsvik, Gaute
- Ulltveit-Moe, Karen Helene
- Vigier, Adrien Henri
- von der Fehr, Nils-Henrik M.
- Wang, Yikai
- Wassie, Solomon Bizuayehu
People who have registered with RePEc and have received their terminal degree from this institution according to the RePEc genealogy:Find all publications of all alumni listed below conveniently compiled on IDEAS. Alumni with publications are listed below with .
- Bhuller, Manudeep
- Claussen, Carl Andreas
- Godager, Geir
- Haavelmo, Trygve
- Han, Xuehui
- Mogstad, Magne
- Sun, Gang
- Vestad, Ola Lotherington
This listing is provided to you by EDIRC at the Economic Research Department, Federal Reserve Bank of St. Louis.
Please send additions and corrections to Christian Zimmermann.


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All the time in the world?
Yes, according to Einstein, I do have all the time in the world, we all do. That means running after the clock might not be working as swimmingly as we would like, but… Continue reading
When I create a new design or a painting, I sometimes have to work against my own mind. I have to force myself to do it different, try a different pen or technique.… Continue reading
I've always been told I am strong and stubborn, not always meant as a compliment, but I usually take it as one. In the eight grade I wrote an essay, and I knew… Continue reading


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text:
Tarpon are among the most sought after saltwater game fish in the Orlando area, Florida is thriving with a healthy population of these silver jumpers or "Silver Kings" as they're often called. What's unusual about these fish is that they have no food value but anglers simply like to catch them for their enormous size and aerobatic fight. Tarpon are known to many anglers as the "Silver King" and when you hook one for the first time you get to know why they're named appropriately.
In the backwaters we often catch baby tarpon under twenty five pounds on both artificial and live baits. Tarpon have unusually hard mouths that have been compared to cinder blocks and remain toothless, but are non-the-less difficult to drive a hook into and extra sharp hooks are a must. Smaller tarpon are often more difficult to hookup than larger ones because of the lack of weight that helps drive the hooks into that formidable jaw, it's often easier to hook them on small sharp tackle and flyrods. Mammoth sized tarpon that are often caught off the beaches and in the inlets can exceed 175 pounds and are generally easier to hookup but difficult to land as it's an endurance test for both angler and fish.
Fights on larger tarpon often exceed two hours on the ocean because the tarpon sound deeper and put more stress on the tackle. Generally speaking a shallow caught tarpon in the lagoon will tire quicker, but the fights are still exciting and may exceed an hour if the fish is over eighty pounds or so. There are fish fighting tactics that can get the job done quicker and with better success. Number One Tactic is to always put the tip of the rod in the water when the fish starts to jump and continue keeping the tip submerged until the fish has completed it's jumping antics. This puts the line in the water and prevents shock waves from jerking the bait or throwing the hook. Many anglers call this method "Bowing to the Silver King", it's a good method and very valid. The second Tactic is to fight your fish against the angle he's swimming while keeping a downward pressure. This method messes up the tarpon's balance and disorients them for a quicker fight.
Anyway around it, Tarpon are a hard fighting and relatively consistent fishery in the Orlando area. If you want a tarpon fishing trip the best times are in the summer and fall in Central Florida's Orlando seashore.
Anglers and Friends,
It's always fun to fishing in the summer east of Orlando, Florida because the beaches and ocean hold such an abundance of fish including the feisty tarpon. On this page you'll see one happy angler holding a nice sized tarpon. "Nice" because it was a small one in tarpon terms only weighing in at less than 30 pounds allowing a short twenty five minute fight and quick release. Tarpon can be brutal and keep anglers preoccupied for hours in the worst conditions and it's often a battle of the will for both angler and fish. Only the strongest make it in large tarpon fights.
If you're down for a summer or fall visit please give us a call and we'll set you up for a day of fishing and if the tarpon are biting... It may be more than you bargained for.
Tight Lines,
Captain Richard Bradley
Lagooner Fishing Guide
Tarpon fishing near Orlando Florida can be found by driving directly east of the Orlando International Airport approximately 38 miles on SR 528/Beach Line. You can find directions on our Maps page to all the inshore fishing areas east of Orlando Florida on our Orlando Fishing Map page.
Fishing Locations (Where to meet your guide)
How Many Anglers on a Fishing Trip
NO FISH NO PAY (warning)
Fishing Mangroves For Gamefish in Orlando
Orlando Deep Sea Fishing King Mackeral
Orlando Fishing Charter Captain Guide
Orlando Family Fishing Charters
Professional Orlando Fishing Guide
Tarpon are slow growers and mature between 7 to 13 years of age. Spawning occurs between May and September when females may lay more than 12 million eggs each. Tarpon can tolerate wide range of salinity from fresh to very salty estuary waters. Juvenile tarpon are commonly found in fresh water and can breathe air at surface. Tarpon feed mainly on fish and large crustaceans.
Summer time is tarpon time in Orlando Florida. Tarpon on on the beaches in packs attacking baitpods and looking to gorge themselves on herring and menhaden. Packing the calories on beach runs of bait fish the pods of bunker and pogies make feeding stations for summertime tarpon off east central Florida. If you're looking to catch a 100 pound tarpon in the Orlando Florida area these are the months to concentrate on when your visiting the sunshine state.


text: We booked a stay at Fite Ranch while visiting Bosque del Apache NWR. As it turned out the time spent at Fite Ranch was every bit as rewarding as our birding on the Bosque. We were accommodated in the Cody Riddle room, the smallest of the four lodgings. However, all four of the "rooms" at Fite Ranch are suites, and... More


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All photos appearing on this site are not to be downloaded or reproduced in ANY way without the written permission of Sustainable Seed Co.
|Organic Dragon Tongue Mustard Seeds
Absolutely one of the most stunning red mustards for a salad mix you will find. Florescent green leaves are highlighted by electric purple edges. Dragon Tongue Mustard has the perfect balance of sweet and spicy so hard to find in mustard. Makes a great stir-fry as well.
|Round Zucchini Seeds
Round Zucchini Seeds


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I might make more of these too... @Erica Wright will have to bring hers if we want to try to show and tell. But I need to make one for ME now!! DIY: Wrap Bracelets - I LOVE this, but I have yet to actually make one for me... I've only made them for friends so far!! I dubbed this one by new go-to birthday gift! OH! And the length was WAY TOO short! Measure it on your wrist before you cut!
DIY bracelet. Made a similar one to the one in the bottom right corner, it almost never leaves my wrist.
quirky painted leather bracelets has a nonstop flow of ideas for different colours , shapes & patterns ;)
Why couldn't I do the nautical knot bracelet and use the gold s hook instead of the anchor???
Braided seed bead bracelett. We did these for girls camp 2012, they were a big hit! They are a little challenging to start but once you get the hang of it they are very easy and fun to do.
Knotted macrame bracelets - these would be really cute with washers and other goodies from the junk drawer, instead of the (admittedly very pretty) goldtone findings in the picture.


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12:43 pm
Thu May 9, 2013
Need A Lift? See Japan's New 'Branomics Bra'
As Japanese Prime Minister Shinzo Abe focuses on boosting his country's bottom line, a lingerie company is hoping to give Japan a different type of lift.
The "Branomics Bra" from Triumph International is a play on Abe's economic policy known as "Abenomics." The company says the garment has a "growth strategy" to help bust Japan's persistent inflation problem, according to Reuters.
The concept bra features three toy arrows and a target shaped pouch, modeled after the prime minister's "three arrows" plan. Business Insider explains Abenomics this way: "The plan ... involves a massive increase in fiscal stimulus through government spending, a massive increase in monetary stimulus through unconventional central bank policy, and a reform program aimed at making structural improvements to the Japanese economy."
Swiss-based Triumph says its creation also has a little something extra up top.
"We put in these pads that boost the bra's cups by 2 percent," Triumph spokeswoman Yoshiko Masuda said, according to UPI. "We hope that as the Japanese economy grows we can also help bust sizes to get bigger."
Abe hopes to bring down Japanese inflation to two percent in two years.
Known for its surprising intimate apparel, Triumph has also produced bras that can be recycled as fuel, the chopstick bra and the husband-hunting bra. But don't look for any of these brassieres – including the Branomics version – in stores. They're not for sale.


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Gunmetal Black Ep 01
episode 14.23 / 5.00 3,884 Views
Lucina's First Day
A look at the first day of Lucina in Super Smash4.23 / 5.00 12,108 Views
BATMAN v SUPERMAN final showdown
SPOILERS!!4.16 / 5.00 11,107 Views
Personally I LOVE Hinata. But i still Found this hilarious. Especialy the part where Naruto turns into the Kyuubi just because Sasuke deosn't love him. Keep up the good work.
You are the best fiori! this is hillarious!!!
Hahaha I laughed so hard when hinata died in the anime. Pain just tossed her body aside .
I didn't like her anyways cause she is so annoying.
Despite the fact I love Hinata...this is funny


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1) Preheat oven to 400 degrees.
2) Line a 12 cup muffin pan with paper liner, spritz with some baking spray and keep aside.
3) In a small bowl, add 1 tablespoon of sugar to the chopped strawberries and set aside.
4) In a large bowl, mix flour, ¾ cup sugar, baking powder, and salt.
5) In a bowl or measuring cup, mix together milk, oil, eggs, and zest. 6)
7) Add to the flour mixture.
8) Drain the juice off the strawberries and fold into batter, mixing until just combined.
9) Using a medium size cookie scoop fill the muffin cups with equal amounts of batter.
10) Bake for 15 minutes.
11) Remove and test for doneness.
12) Leave the muffins in the pan for 5 minutes and then transfer to cooling rack and allow to cool.
13) Mix softened butter and a dollop of jam to make the strawberry butter.
14) Serve with the Strawberry butter.


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Like a ton of bricksStefan Hard / Staff Photo
Pedestrians use a sidewalk detour around an area in front of Aubuchon Hardware on Main Street in Montpelier where bricks continue to fall from the top of French's Block. The first signs of trouble came Sunday when a long board and the first bricks came tumbling down.
MONTPELIER — Fire Chief Robert Gowans said Tuesday that it will be the end of the week before temporary repairs take place to stabilize the facade of the French's building downtown on Main Street. The city closed down the street and sidewalk in front of the building as well as the alleyway between Bagitos and the beginning of the block.
"Yesterday DeWolfe (Engineering Associates) sent engineers who examined the building and removed anything that was loose or unstable," said Gowans. "They will come back in two days to reassess the situation and we hope that everything will be stable enough that we can open up the street and sidewalk at that point."
The closure affects three storefronts: Aubuchon Hardware, whose corporate parent owns the building, Petals and Things, and Capitol Copy. All three businesses have entries at the rear of the building.
An assistant manager at Aubuchon declined to comment on the situation. Gowans indicated that further work on the facade will have to take place, but that they are hopeful that when it comes time to do the bigger job, scaffolding will be set up in such a way to allow them to keep the sidewalks and storefronts open.MORE IN Central VermontMONTPELIER The permit to spray the chemical herbicide glyphosate on the railroad track in the... Full StoryA Marshfield man is accused of threatening to hit another man with brass knuckles after being... Full StoryBRATTLEBORO A distributor in a large New York-to-Rutland heroin network will serve five years... Full Story
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The sad truth of VoIP today – unless you are dialing a lot of long distance to other countries, VoIP services are not worth the effort. The New York Times has the details, though somehow I find the assertions of Vince Vittore, executive editor of Telephony magazine that Vonage has half the VoIP market as not true. Telegeography had put the estimated VoIP callers in US at 1.8 million. Vonage has 800,000 or so, but that doesn't mean 50%.
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I may be totally off my rocker but I thought that having a gallery meant that when you clicked on one of the images, you would have the option to click through to the next image or previous image through there. Was this just a plugin function?
I am working on a website that I did not set up. It has a custom theme. Here is an example of a photo page where I want the gallery to be slideshow (not automatic but clickable).
Please make any suggestions.
Thanks - Jennifer


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Hollywood keeps us on the edge of our seats with action movies, from James Bond to Jason Bourne.
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Sleeping Cancer Can 'Wake Up' Years LaterMay 29, 2015 9:07 am | by Seth Augenstein, Digital Reporter | News | Comments
Years, even decades, after remission some cancers return without warning. The "sleeping" cancer cells reactivate, "waking up" decades later, according to a British team of scientists, who say they may have found the molecular key to the change.
Diagnosing Cancer With Help from BacteriaMay 28, 2015 9:56 am | by Anne Trafton, MIT | News | Comments
Engineered probiotics can detect tumors in the liver.
Zebrafish Model Gives New Insight on Autism Spectrum DisorderMay 28, 2015 9:46 am | by University of Miami | News | Comments
Study of zebrafish reveals how dysfunction of SHANK3 or SYNGAP1 genes play a role in the development of Autism Spectrum Disorder.
23andMe's Plan to Harness Data for Disease TreatmentsMay 28, 2015 8:59 am | by Ryan Bushey, Associate Editor | Articles | Comments
The company's massive database of genetic information it collected over the years will play an important role in this new project.
Imaging Test May Identify Biomarker of Alzheimer's DiseaseMay 28, 2015 8:59 am | by Radiological Society of North America | News | Comments
Degeneration of the white matter of the brain may be an early marker of specific types of Alzheimer's disease (AD), including early-onset AD, according to results of a new study published in the journal Radiology.
Scientists Tout New 'Rosetta Stone' for Prostate Cancer MutationsMay 27, 2015 10:18 am | by Seth Augenstein, Digital Reporter | News | Comments
About 90 percent of advanced prostate cancers have particular genetic mutations that can provide a target for cancer drugs. An international team of scientists say they have cataloged a comprehensive map of those mutations in metastatic prostate cancers, in a paper published in the journal Cell.
Tiny Heart, Big PromiseMay 27, 2015 9:56 am | by Children's Hospital Los Angeles | News | Comments
Understanding how cells become coronary vessels may lead to advances in repairing heart damage .
New Chip Makes Testing for Antibiotic-resistant Bacteria Faster, EasierMay 27, 2015 9:23 am | by University of Toronto | News | Comments
Researchers have designed a small and simple chip to test for antibiotic resistance in just one hour, giving doctors a shot at picking the most effective antibiotic to treat potentially deadly infections. Their work was was published this week in the international journal Lab on a Chip.
Scientists are Designing Decoy Drugs to Fool CancerMay 27, 2015 9:06 am | by Columbia University | News | Comments
Cancer cells are shifty characters. They use a number of dirty tricks to survive and infiltrate the body. Now scientists are fighting back with some sneaky strategies of their own. A study published in Cancer Discovery describes how researchers have created new decoy drugs that can intercept the deceptive growth signals that cancer cells send out.
Master Gene Regulator Could be New Target for Schizophrenia TreatmentMay 26, 2015 10:12 am | by MIT | News | Comments
Researchers have identified a master genetic regulator that could account for faulty brain functions that contribute to schizophrenia.
WHO Addresses Antimicrobial Resistance, Immunization Gaps, and MalnutritionMay 26, 2015 10:05 am | by World Health Organization | News | Comments
The World Health Assembly agreed on resolutions to tackle antimicrobial resistance; improve access to affordable vaccines and address over- and under-nutrition.
'Pain-sensing' Gene Discovery Could Aid Development of New Pain TreatmentsMay 26, 2015 9:40 am | by University of Cambridge | News | Comments
A gene essential to the production of pain-sensing neurons in humans has been identified by an international team of researchers. The discovery, reported in the journal Nature Genetics, could have implications for the development of new methods of pain relief.
Tiny Spheres of Human Cells Mimic the BrainMay 26, 2015 9:22 am | by Stanford University | News | Comments
Researchers have figured out how to create spheres of neuronal cells resembling the cerebral cortex, making functional human brain tissue available for the first time to study neuropsychiatric diseases such as autism and schizophrenia
Peeking into Healthy Brains to See if Alzheimer's is BrewingMay 26, 2015 9:08 am | by Lauran Neergaard, AP Medical Writer | News | Comments
Sticky plaque gets the most attention, but now healthy seniors at risk of Alzheimer's are letting scientists peek into their brains to see if another culprit is lurking.
Proteins May Slow Memory Loss in People With Alzheimer'sMay 22, 2015 10:04 am | by Iowa State University | News | Comments
Certain proteins may slow the devastating memory loss caused by Alzheimer's disease, according to a study.
Seeking Deeper Understanding of How the Brain WorksMay 22, 2015 9:17 am | by MIT | News | Comments
Edward Boyden develops techniques to study the brain, and how it operates, in finer detail.
Women With MRI Abnormality Nine Times More Likely to Get Breast CancerMay 22, 2015 9:11 am | by Cynthia Fox, Science Writer | Articles | Comments
Healthy women possessing a certain abnormality on MRIs are nine times more likely to get breast cancer, according to research published in Radiology. The abnormality is called background parenchymal enhancement (BPE), a phenomenon in which areas of normal background breast tissue appear white, or enhanced.
Scientists Figure Out How Vitamin E Keeps Muscles HealthyMay 21, 2015 10:57 am | by Georgia Regents University | News | Comments
Body builders have it right: vitamin E does help build strong muscles, and scientists appear to have figured out one important way it does it.
Uncovering Mechanisms of Replication in HPVMay 21, 2015 10:28 am | by Northwestern University | News | Comments
Scientists have identified proteins that mediate aspects of virus replication in the lifecycle of human papillomavirus (HPV), a finding that may lead to new therapeutic targets for treatment of infections caused by the virus.
Shining a New Light on the Immune SystemMay 21, 2015 9:45 am | by University of St. Andrews | News | Comments
Scientists have developed a revolutionary method of identifying cells of the immune system with "molecular fingerprints" which could pave the way for the rapid detection of conditions such as leukemia and lymphoma from a small blood sample.
Promising Malaria and Dengue Vaccines Will Not Defeat DiseasesMay 21, 2015 9:02 am | by Christopher J. Pace, Ph.D., GlobalData Managing Analyst, Infectious Diseases | Articles | Comments
The company expects that while vaccines will be an essential component of future dengue and malaria prevention and control efforts, immunization cannot succeed as a silver bullet solution for either disease.
Study Explains How Early Childhood Vaccination Reduces Leukemia RiskMay 20, 2015 10:09 am | by University of California San Francisco | News | Comments
A team of researchers has discovered how a commonly administered vaccine protects against acute lymphoblastic leukemia (ALL), the most common type of childhood cancer.
Researchers Identify Potentially Effective Treatment for Methamphetamine AddictionMay 20, 2015 10:07 am | by UCLA | News | Comments
A new study has found that Naltrexone, a drug used to treat alcoholism, may also be a promising treatment for addiction to methamphetamine.
New Technology Turns Smartphone into a DNA-Scanning MicroscopeMay 20, 2015 10:02 am | by Bevin Fletcher, Associate Editor | Articles | Comments
Researchers at University of California, Los Angeles (UCLA) have developed a new technology that turns a smartphone into a DNA-scanning fluorescent microscope. Lead researcher Aydogan Ozcan, Howard Hughes Medical Institute chancellor professor at UCLA, sat down with Bioscience Technology to talk about this advancement and its implications for resource-poor labs, and for personalized medicine.
Abcodia Raises $8M to Launch Ovarian Cancer Screening TestMay 20, 2015 8:35 am | by Ryan Bushey, Associate Editor | Articles | Comments
Abcodia announced this week it raised $8 million to bring its ovarian cancer screening test called ROCA to market.


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A little too tame for CMJ, Mike Bones would have fit in better opening on a club tour for a band like Sian Alice Group rather than playing to a crowd that seemed to be hyping themselves up for Gang Gang Dance. I wasn't sure how I felt about him before this show, but he really is an inimitable songwriter, and it's less about the actual music and more about his clever lyricism. I'd be interested to see him take a full band out on the road and continue to make records on a bare bones level.
Topics: cmj | mike bones
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Are you Nathaniel J Cosper?Claim your profile
Publications (25)99.62 Total impact
- SourceAvailable from: Robert A Scott[Show abstract] [Hide abstract]
ABSTRACT: Proteins containing Rieske-type [2Fe-2S] clusters play essential functions in all three domains of life. We engineered the two histidine ligands to the Rieske-type [2Fe-2S] cluster in the hyperthermophilic archaeal Rieske-type ferredoxin from Sulfolobus solfataricus to modify types and spacing of ligands and successfully converted the metal and cluster type at the redox-active site with a minimal structural change to a native Rieske-type protein scaffold. Spectroscopic analyses unambiguously established a rubredoxin-type mononuclear Fe3+/2+ center at the engineered local metal-binding site (Zn2+ occupies the iron site depending on the expression conditions). These results show the importance of types and spacing of ligands in the in vivo cluster recognition/insertion/assembly in biological metallosulfur protein scaffolds. We suggest that early ligand substitution and displacement events at the local metal-binding site(s) might have primarily allowed the metal and cluster type conversion in ancestral redox protein modules, which greatly enhanced their capabilities of conducting a wide range of unique redox chemistry in biological electron transfer conduits, using a limited number of basic protein scaffolds.Journal of Biological Chemistry 04/2005; 280(10):9129-34. DOI:10.1074/jbc.M414051200 · 4.60 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Structural and functional characterization of the entire protein complement (the proteome) of an organism can provide an infrastructure upon which questions about biological pathways and systems biology can be framed. The technology necessary to perform this proteome-level structural and functional characterization is under development in numerous structural genomics and functional genomics initiatives. Given the ubiquity of metal active sites in a proteome, it seems appropriate to ask whether comprehensive local structural characterization of metal sites within a proteome (metalloproteomics) is either a valid or obtainable goal. With a proteome-wide knowledge of the active-site structures of all metalloproteins, one could start to ask how metal insertion, cluster assembly and metalloprotein expression are affected by growth conditions or developmental status etc. High-throughput X-ray absorption spectroscopy (HTXAS) is being developed as a technology for investigating the metalloproteome. In creating a pipeline from genome to metalloproteome, several bottlenecks to high-throughput determination of metal-site structures must be overcome. For example, automation of arraying small samples for XAS examination must be invented, automation of rapid data collection of multiple low-volume low-concentration samples must be developed, automation of data reduction and analysis must be perfected. Discussed here are the promises and the pitfalls of HTXAS development, including the results of initial feasibility experiments.Journal of Synchrotron Radiation 02/2005; 12(Pt 1):19-22. DOI:10.1107/S0909049504028791 · 3.02 Impact Factor
Article: A stable mercury-containing complex of the organomercurial lyase MerB: Catalysis, product release, and direct transfer to MerA[Show abstract] [Hide abstract]
ABSTRACT: Bacteria isolated from organic mercury-contaminated sites have developed a system of two enzymes that allows them to efficiently convert both ionic and organic mercury compounds to the less toxic elemental mercury. Both enzymes are encoded on the mer operon and require sulfhydryl-bound substrates. The first enzyme is an organomercurial lyase (MerB), and the second enzyme is a mercuric ion reductase (MerA). MerB catalyzes the protonolysis of the carbon-mercury bond, resulting in the formation of a reduced carbon compound and inorganic ionic mercury. Of several mercury-containing MerB complexes that we attempted to prepare, the most stable was a complex consisting of the organomercurial lyase (MerB), a mercuric ion, and a molecule of the MerB inhibitor dithiothreitol (DTT). Nuclear magnetic resonance (NMR) spectroscopy and extended X-ray absorption fine structure spectroscopy of the MerB/Hg/DTT complex have shown that the ligands to the mercuric ion in the complex consist of both sulfurs from the DTT molecule and one cysteine ligand, C96, from the protein. The stability of the MerB/Hg/DTT complex, even in the presence of a large excess of competing cysteine, has been demonstrated by NMR and dialysis. We used an enzyme buffering test to determine that the MerB/Hg/DTT complex acts as a substrate for the mercuric reductase MerA. The observed MerA activity is higher than the expected activity assuming free diffusion of the mercuric ion from MerB to MerA. This suggests that the mercuric ion can be transferred between the two enzymes by a direct transfer mechanism.Biochemistry 08/2004; 43(26):8333-45. DOI:10.1021/bi049662h · 3.19 Impact Factor
Article: Engineering a three-cysteine, one-histidine ligand environment into a new hyperthermophilic archaeal Rieske-type [2Fe-2S] ferredoxin from Sulfolobus solfataricus[Show abstract] [Hide abstract]
ABSTRACT: We heterologously overproduced a hyperthermostable archaeal low potential (E(m) = -62 mV) Rieske-type ferredoxin (ARF) from Sulfolobus solfataricus strain P-1 and its variants in Escherichia coli to examine the influence of ligand substitutions on the properties of the [2Fe-2S] cluster. While two cysteine ligand residues (Cys(42) and Cys(61)) are essential for the cluster assembly and/or stability, the contributions of the two histidine ligands to the cluster assembly in the archaeal Rieske-type ferredoxin appear to be inequivalent as indicated by much higher stability of the His(64) --> Cys variant (H64C) than the His(44) --> Cys variant (H44C). The x-ray absorption and resonance Raman spectra of the H64C variant firmly established the formation of a novel, oxidized [2Fe-2S] cluster with one histidine and three cysteine ligands in the archaeal Rieske-type protein moiety. Comparative resonance Raman features of the wild-type, natural abundance and uniformly (15)N-labeled ARF and its H64C variant showed significant mixing of the Fe-S and Fe-N stretching characters for an oxidized biological [2Fe-2S] cluster with partial histidine ligation.Journal of Biological Chemistry 03/2004; 279(13):12519-28. DOI:10.1074/jbc.M305923200 · 4.60 Impact Factor
Article: The dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase from haemophilus influenzae is a dinuclear metallohydrolase[Show abstract] [Hide abstract]
ABSTRACT: The Zn K-edge extended X-ray absorption fine structure (EXAFS) spectra, of the dapE-encoded N-succinyl-l,l-diaminopimelic acid desuccinylase (DapE) from Haemophilus influenzae have been recorded in the presence of one or two equivalents of Zn(II) (i.e. [Zn_(DapE)] and [ZnZn(DapE)]). The Fourier transforms of the Zn EXAFS are dominated by a peak at ca. 2.0 A, which can be fit for both [Zn_(DapE)] and [ZnZn(DapE)], assuming ca. 5 (N,O) scatterers at 1.96 and 1.98 A, respectively. A second-shell feature at ca. 3.34 A appears in the [ZnZn(DapE)] EXAFS spectrum but is significantly diminished in [Zn_(DapE)]. These data show that DapE contains a dinuclear Zn(II) active site. Since no X-ray crystallographic data are available for any DapE enzyme, these data provide the first glimpse at the active site of DapE enzymes. In addition, the EXAFS data for DapE incubated with two competitive inhibitors, 2-carboxyethylphosphonic acid and 5-mercaptopentanoic acid, are also presented.Journal of the American Chemical Society 01/2004; 125(48):14654-5. DOI:10.1021/ja036650v · 11.44 Impact Factor
Article: Structural studies of the interaction of S-adenosylmethionine with the [4Fe-4S] clusters in biotin synthase and pyruvate formate-lyase activating enzyme.[Show abstract] [Hide abstract]
ABSTRACT: The diverse reactions catalyzed by the radical-SAM superfamily of enzymes are thought to proceed via a set of common mechanistic steps, key among which is the reductive cleavage of S-adenosyl-L-methionine (SAM) by a reduced [4Fe-4S] cluster to generate an intermediate deoxyadenosyl radical. A number of spectroscopic studies have provided evidence that SAM interacts directly with the [4Fe-4S] clusters in several of the radical-SAM enzymes; however, the molecular mechanism for the reductive cleavage has yet to be elucidated. Selenium X-ray absorption spectroscopy (Se-XAS) was used previously to provide evidence for a close interaction between the Se atom of selenomethionine (a cleavage product of Se-SAM) and an Fe atom of the [4Fe-4S] cluster of lysine-2,3-aminomutase (KAM). Here, we utilize the same approach to investigate the possibility of a similar interaction in pyruvate formate-lyase activating enzyme (PFL-AE) and biotin synthase (BioB), two additional members of the radical-SAM superfamily. The results show that the latter two enzymes do not exhibit the same Fe-Se interaction as was observed in KAM, indicating that the methionine product of reductive cleavage of SAM does not occupy a well-defined site close to the cluster in PFL-AE and BioB. These results are interpreted in terms of the differences among these enzymes in their use of SAM as either a cofactor or a substrate.Protein Science 08/2003; 12(7):1573-7. DOI:10.1110/ps.0302203 · 2.86 Impact Factor
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ABSTRACT: Staphylococcus aureus CzrA and Mycobacterium tuberculosis NmtR are homologous zinccobalt-responsive and nickelcobalt-responsive transcriptional repressors in vivo, respectively, and members of the ArsRSmtB superfamily of prokaryotic metal sensor proteins. We show here that Zn(II) is the most potent negative allosteric regulator of czr operatorpromoter binding in vitro with the trend Zn(II)>Co(II)Ni(II), whereas the opposite holds for the binding of NmtR to the nmt operatorpromoter, Ni(II)>Co(II)>Zn(II). Characterization of the metal coordination complexes of CzrA and NmtR by UVvisible and x-ray absorption spectroscopies reveals that metals that form four-coordinate tetrahedral complexes with CzrA [Zn(II) and Co(II)] are potent regulators of DNA binding, whereas metals that form five- or six-coordinate complexes with NmtR [Ni(II) and Co(II)] are the strongest allosteric regulators in this system. Strikingly, the Zn(II) coordination complexes of CzrA and NmtR cannot be distinguished from one another by x-ray absorption spectroscopy, with the best fit a His-3-carboxylate complex in both cases. Inspection of the primary structures of CzrA and NmtR, coupled with previous functional data, suggests that three conserved His and one Asp from the C-terminal alpha5 helix donate ligands to create a four-coordinate complex in both CzrA and NmtR, with NmtR uniquely capable of expanding its coordination number in the Ni(II) and Co(II) complexes by recruiting additional His ligands from a C-terminal extension of the alpha5 helix.Proceedings of the National Academy of Sciences 04/2003; 100(7):3713-8. DOI:10.1073/pnas.0636943100 · 9.81 Impact Factor
Article: Coordination and geometry of the nickel atom in active methyl-coenzyme M reductase from Methanothermobacter marburgensis as detected by X-ray absorption spectroscopy[Show abstract] [Hide abstract]
ABSTRACT: Methyl-coenzyme M reductase (MCR) catalyzes the reduction of methyl-coenzyme M (CH(3)-S-CoM) to methane. The enzyme contains as a prosthetic group the nickel porphinoid F(430) which in the active enzyme is in the EPR-detectable Ni(I) oxidation state. Crystal structures of several inactive Ni(II) forms of the enzyme but not of the active Ni(I) form have been reported. To obtain structural information on the active enzyme-substrate complex we have now acquired X-ray absorption spectra of active MCR in the presence of either CH(3)-S-CoM or the substrate analog coenzyme M (HS-CoM). For both MCR complexes the results are indicative of the presence of a five-coordinate Ni(I), the five ligands assigned as four nitrogen ligands from F(430) and one oxygen ligand. Analysis of the spectra did not require the presence of a sulfur ligand indicating that CH(3)-S-CoM and HS-CoM were not coordinated via their sulfur atom to nickel in detectable amounts. As a control, X-ray absorption spectra were evaluated of three enzymatically inactive MCR forms, MCR-silent, MCR-ox1-silent and MCR-ox1, in which the nickel is known to be six-coordinate. Comparison of the edge position of the X-ray absorption spectra revealed that the Ni(I) in the active enzyme is more reduced than the Ni in the two EPR-silent Ni(II) states. Surprisingly, the edge position of the EPR-active MCR-ox1 state was found to be the same as that of the two silent states indicating similar electron density on the nickel.JBIC Journal of Biological Inorganic Chemistry 02/2003; 8(1-2):141-8. DOI:10.1007/s00775-002-0399-2 · 3.16 Impact Factor
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ABSTRACT: Proteins containing Rieske-type [2Fe-2S] clusters play important roles in many biological electron transfer reactions. Typically, [2Fe-2S] clusters are not directly involved in the catalytic transformation of substrate, but rather supply electrons to the active site. We report herein X-ray absorption spectroscopic (XAS) data that directly demonstrate an average increase in the iron-histidine bond length of at least 0.1 A upon reduction of two distantly related Rieske-type clusters in archaeal Rieske ferredoxin from Sulfolobus solfataricus strain P-1 and bacterial anthranilate dioxygenases from Acinetobacter sp. strain ADP1. This localized redox-dependent structural change may fine tune the protein-protein interaction (in the case of ARF) or the interdomain interaction (in AntDO) to facilitate rapid electron transfer between a lower potential Rieske-type cluster and its redox partners, thereby regulating overall oxygenase reactions in the cells.Protein Science 01/2003; 11(12):2969-73. DOI:10.1110/ps.0222402 · 2.86 Impact Factor
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ABSTRACT: Manganese(II) activation of the methionyl aminopeptidases from Escherichia coli (EcMetAP-I) and the hyperthermophilic archaeon Pyrococcus furiosus (PfMetAP-II) was investigated. Maximum catalytic activity for both enzymes was obtained with 1 equiv of Mn(II), and the dissociation constants (K(d)) for the first metal binding site were found to be 6 +/- 0.5 and 1 +/- 0.5 microM for EcMetAP-I and PfMetAP-II, respectively. These K(d) values were verified by isothermal titration calorimetry (ITC) and found to be 3.0 +/- 0.2 and 1.4 +/- 0.2 microM for EcMetAP-I and PfMetAP-II, respectively. The hydrolysis of MGMM was measured in triplicate between 25 and 85 degrees C at eight substrate concentrations ranging from 2 to 20 mM for PfMetAP-II. Both specific activity and K(m) values increased with increasing temperature. An Arrhenius plot was constructed from the kcat values and was found to be linear over the temperature range 25-85 degrees C. The activation energy for the Mn(II)-loaded PfMetAP-II hydrolysis of MGMM was found to be 25.7 kJ/mol while the remaining thermodynamic parameters calculated at 25 degrees C are DeltaG+ = 50.1 kJ/mol, DeltaH+ = 23.2 kJ/mol, and DeltaS++ = -90.2 J x mol(-1) x K(-1).Biochemistry 11/2002; 41(43):13096-105. DOI:10.1021/bi020395u · 3.19 Impact Factor
Article: Novel [2Fe-2S]-type redox center C in SdhC of archaeal respiratory complex II from Sulfolobus tokodaii strain 7[Show abstract] [Hide abstract]
ABSTRACT: The SdhC subunit of the archaeal respiratory complex II (succinate:quinone oxidoreductase) from Sulfolobus tokodaii strain 7 has a novel cysteine rich motif and is also related to archaeal and bacterial heterodisulfide reductase subunits. We overexpressed the sdhC gene heterologously in Escherichia coli and characterized the gene product in greater detail. Low temperature resonance Raman and x-ray absorption spectroscopic investigation collectively demonstrate the presence of a [2Fe-2S] cluster core with complete cysteinyl ligation (Center C) and an isolated zinc site in the recombinant SdhC. The [2Fe-2S]2+ cluster core is sensitive to dithionite, resulting in irreversible breakdown of the Fe-Fe interaction. EPR analysis confirmed that the novel Center C is an inherent redox center in the archaeal complex II, showing unique EPR signals in the succinate-reduced state. Distinct subunit and cofactor arrangements in the S. tokodaii respiratory complex II, as compared with those in mitochondrial and some mesophilic bacterial enzymes, indicate modular evolution of this ubiquitous electron entry site in the respiratory chains of aerobic organisms.Journal of Biological Chemistry 11/2002; 277(42):39642-8. DOI:10.1074/jbc.M207312200 · 4.60 Impact Factor
Article: Structural evidence that the methionyl aminopeptidase from Escherichia coli is a mononuclear metalloprotease[Show abstract] [Hide abstract]
ABSTRACT: The Co and Fe K-edge extended X-ray absorption fine structure (EXAFS) spectra of the methionyl aminopeptidase from Escherichia coli (EcMetAP) have been recorded in the presence of 1 and 2 equiv of either Co(II) or Fe(II) (i.e., [Co(II)_(EcMetAP)], [Co(II)Co(II)(EcMetAP)], [Fe(II)_(EcMetAP)], and [Fe(II)Fe(II)(EcMetAP)]). The Fourier transformed data of both [Co(II)_(EcMetAP)] and [Co(II)Co(II)(EcMetAP)] are dominated by a peak at ca. 2.05 A, which can be fit assuming 5 light atom (N,O) scatterers at 2.04 A. Attempts to include a Co-Co interaction (in the 2.4-4.0 A range) in the curve-fitting parameters were unsuccessful. Inclusion of multiple-scattering contributions from the outer-shell atoms of a histidine-imidazole ring resulted in reasonable Debye-Waller factors for these contributions and a slight reduction in the goodness-of-fit value (f '). These data suggest that a dinuclear Co(II) center does not exist in EcMetAP and that the first Co atom is located in the histidine-ligated side of the active site. The EXAFS data obtained for [Fe(II)_(EcMetAP)] and [Fe(II)Fe(II)(EcMetAP)] indicate that Fe(II) binds to EcMetAP in a similar site to Co(II). Since no X-ray crystallographic data are available for any Fe(II)-substituted EcMetAP enzyme, these data provide the first glimpse at the Fe(II) active site of MetAP enzymes. In addition, the EXAFS data for [Co(II)Co(II)(EcMetAP)] incubated with the antiangiogenesis drug fumagillin are also presented.Biochemistry 12/2001; 40(44):13302-9. DOI:10.1021/bi010837m · 3.19 Impact Factor
Article: X-ray absorption spectroscopic analysis of the high-spin ferriheme site in substrate-bound neuronal nitric-oxide synthase.[Show abstract] [Hide abstract]
ABSTRACT: Nitric oxide synthase (NOS) catalyzes the conversion of L-arginine to citrulline and nitric oxide through two stepwise oxygenation reactions involving N(omega)-hydroxy-L-arginine, an enzyme-bound intermediate. The N(omega)-hydroxy-L-arginine- and arginine-bound NOS ferriheme centers show distinct, high-spin electron paramagnetic resonance signals. Iron X-ray absorption spectroscopy (XAS) has been used to examine the structure of the ferriheme site in the N(omega)-hydroxy-L-arginine-bound full-length neuronal NOS in the presence of (6R)-5,6,7,8-tetrahydro-L-biopterin. Iron XAS shows that the high-spin ferriheme sites in the N(omega)-hydroxy-L-arginine- and arginine-bound forms are strikingly similar, both being coordinated by the heme and an axial thiolate ligand, with an Fe-S distance of ca. 2.29 A. Cu(2+) inhibition slightly affects the spin-state equilibrium, but causes no XAS-detectable changes in the immediate ferriheme coordination environment of neuronal NOS. The structure and ligand geometry of the high-spin ferriheme in arginine-bound neuronal NOS are essentially identical to those of the N(omega)-hydroxy-L-arginine-bound form and only slightly affected by the divalent cation inhibitor of constitutive NOS.Journal of Biochemistry 09/2001; 130(2):191-8. DOI:10.1093/oxfordjournals.jbchem.a002972 · 3.07 Impact Factor
Article: Spectroscopic properties of the metalloregulatory Cd(II) and Pb(II) sites of S. aureus pI258 CadC[Show abstract] [Hide abstract]
ABSTRACT: Staphylococcus aureus pI258 CadC is an extrachromosomally encoded metalloregulatory repressor protein from the ArsR superfamily which negatively regulates the expression of the cad operon in a metal-dependent fashion. The metalloregulatory hypothesis holds that direct binding of thiophilic divalent cations including Cd(II), Pb(II), and Zn(II) by CadC allosterically regulates the DNA binding activity of CadC to the cad operator/promoter (O/P). This report presents a detailed characterization of the metal binding and DNA binding properties of wild-type CadC. The results of analytical ultracentrifugation experiments suggest that both apo- and Cd(1)-CadC are stable or weakly dissociable homodimers characterized by a K(dimer) = 3.0 x 10(6) M(-1) (pH 7.0, 0.20 M NaCl, 25.0 degrees C) with little detectable effect of Cd(II) on the dimerization equilibrium. As determined by optical spectroscopy, the stoichiometry of Cd(II) and Pb(II) binding is approximately 0.7-0.8 mol/mol of wild-type CadC monomer. Chelator (EDTA) competition binding isotherms reveal that Cd(II) binds very tightly, with K(Cd) = 4.3 (+/-1.8) x 10(12) M(-1). The results of UV-Vis and X-ray absorption spectroscopy of the Cd(1) complex are consistent with a tetrathiolate (S(4)) complex formed by four cysteine ligands. The (113)Cd NMR spectrum reveals a single resonance of delta = 622 ppm, consistent with an S(3)(N,O) or unusual upfield-shifted S(4) complex. The Pb(II) complex reveals two prominent absorption bands at 350 nm (epsilon = 4000 M(-1) cm(-1)) and 250 nm (epsilon = 41 000 M(-1) cm(-1)), spectral properties consistent with three or four thiolate ligands to the Pb(II) ion. The change in the anisotropy of a fluorescein-labeled oligonucleotide containing the cad O/P upon binding CadC and analyzed using a dissociable CadC dimer binding model reveals that apo-CadC forms a high-affinity complex [K(a) = (1.1 +/- 0.3) x 10(9) M(-1); pH 7.0, 0.40 M NaCl, 25 degrees C], the affinity of which is reduced approximately 300-fold upon the binding of a single molar equivalent of Cd(II) or Pb(II). The implications of these findings on the mechanism of metalloregulation are discussed.Biochemistry 05/2001; 40(14):4426-36. DOI:10.1021/bi010006g · 3.19 Impact Factor
Article: Expression, purification, and characterization of NosL, a novel Cu(II) protein of the nitrous oxide reductase (nos) gene cluster[Show abstract] [Hide abstract]
ABSTRACT: NosL, one of the accessory proteins of the nos (nitrous oxide reductase) gene cluster, has been heterologously expressed, purified, and characterized. NosL is a monomeric protein of 18,540 MW that specifically and stoichiometrically binds Cu(I). The copper ion in NosL is ligated by a Cys residue, and one Met and one His are thought to serve as the other ligands. While it is possible to oxidize Cu(I)-NosL with ferricyanide, the Cu(II) ion thus formed appears to dissociate from the protein. The function of Cu(I)NosL is not yet known, but the data indicate that NosL does not act as an electron transfer partner to nitrous oxide reductase. NosL is encoded on the same transcript as three other gene products (NosD, NosF, and NosY). These have been shown to be required for assembly of the active site in nitrous oxide reductase, which is thought to be a copper cluster. Accordingly, it is possible that NosL is a copper chaperone involved in metallocenter assembly.JBIC Journal of Biological Inorganic Chemistry 03/2001; 6(2):189-95. DOI:10.1007/s007750000190 · 3.16 Impact Factor
Article: Mutations in catB, the gene encoding muconate cycloisomerase, activate transcription of the distal ben genes and contribute to a complex regulatory circuit in Acinetobacter sp. strain ADP1.[Show abstract] [Hide abstract]
ABSTRACT: Mutants of the bacterium Acinetobacter sp. strain ADP1 were selected to grow on benzoate without the BenM transcriptional activator. In the wild type, BenM responds to benzoate and cis,cis-muconate to activate expression of the benABCDE operon, which is involved in benzoate catabolism. This operon encodes enzymes that convert benzoate to catechol, a compound subsequently degraded by cat gene-encoded enzymes. In this report, four spontaneous mutants were found to carry catB mutations that enabled BenM-independent growth on benzoate. catB encodes muconate cycloisomerase, an enzyme required for benzoate catabolism. Its substrate, cis,cis-muconate, is enzymatically produced from catechol by the catA-encoded catechol 1,2-dioxygenase. Muconate cycloisomerase was purified to homogeneity from the wild type and the catB mutants. Each purified enzyme was active, although there were differences in the catalytic properties of the wild type and variant muconate cycloisomerases. Strains with a chromosomal benA::lacZ transcriptional fusion were constructed and used to investigate how catB mutations affect growth on benzoate. All of the catB mutations increased cis,cis-muconate-activated ben gene expression in strains lacking BenM. A model is presented in which the catB mutations reduce muconate cycloisomerase activity during growth on benzoate, thereby increasing intracellular cis, cis-muconate concentrations. This, in turn, may allow CatM, an activator similar to BenM in sequence and function, to activate ben gene transcription. CatM normally responds to cis,cis-muconate to activate cat gene expression. Consistent with this model, muconate cylcoisomerase specific activities in cell extracts of benzoate-grown catB mutants were low relative to that of the wild type. Moreover, the catechol 1,2-dioxygenase activities of the mutants were elevated, which may result from CatM responding to the altered intracellular levels of cis,cis-muconate and increasing catA expression. Collectively, these results support the important role of metabolite concentrations in controlling benzoate degradation via a complex transcriptional regulatory circuit.Journal of Bacteriology 01/2001; 182(24):7044-52. DOI:10.1128/JB.182.24.7044-7052.2000 · 2.69 Impact Factor
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ABSTRACT: Lysine 2,3-aminomutase (KAM) belongs to a class of enzymes that use FeS clusters and S-adenosyl-L-methionine to initiate radical-dependent chemistry. Selenium K-edge X-ray absorption spectroscopic analysis of KAM poised at various stages of catalysis, in the presence of selenomethionine or Se-adenosyl-L-selenomethionine, reveals that the cofactor is cleaved only in the presence of dithionite and the substrate analogue trans-4,5-dehydrolysine. A new Fourier transform peak at 2.7 A, assigned as a Se-Fe interaction, appears concomitant with this cleavage. This is the first demonstration of a direct interaction of S-adenosyl-L-methionine, or its cleavage products, with the FeS cluster in this class of enzymes.Biochemistry 01/2001; 39(51):15668-73. DOI:10.1021/bi0022184 · 3.19 Impact Factor
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ABSTRACT: The beta-class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum (Cab) was structurally and kinetically characterized. Analytical ultracentrifugation experiments show that Cab is a tetramer. Circular dichroism studies of Cab and the Spinacia oleracea (spinach) beta-class carbonic anhydrase indicate that the secondary structure of the beta-class enzymes is predominantly alpha-helical, unlike that of the alpha- or gamma-class enzymes. Extended X-ray absorption fine structure results indicate the active zinc site of Cab is coordinated by two sulfur and two O/N ligands, with the possibility that one of the O/N ligands is derived from histidine and the other from water. Both the steady-state parameters k(cat) and k(cat)/K(m) for CO(2) hydration are pH dependent. The steady-state parameter k(cat) is buffer-dependent in a saturable manner at both pH 8.5 and 6.5, and the analysis suggested a ping-pong mechanism in which buffer is the second substrate. At saturating buffer conditions and pH 8.5, k(cat) is 2.1-fold higher in H(2)O than in D(2)O, consistent with an intramolecular proton transfer step being rate contributing. The steady-state parameter k(cat)/K(m) is not dependent on buffer, and no solvent hydrogen isotope effect was observed. The results suggest a zinc hydroxide mechanism for Cab. The overall results indicate that prokaryotic beta-class carbonic anhydrases have fundamental characteristics similar to the eukaryotic beta-class enzymes and firmly establish that the alpha-, beta-, and gamma-classes are convergently evolved enzymes that, although structurally distinct, are functionally equivalent.Journal of Bacteriology 01/2001; 182(23):6605-13. DOI:10.1128/JB.182.23.6605-6613.2000 · 2.69 Impact Factor
Article: Spectroscopic investigation of selective cluster conversion of archaeal zinc-containing ferredoxin from Sulfolobus sp strain 7[Show abstract] [Hide abstract]
ABSTRACT: Archaeal zinc-containing ferredoxin from Sulfolobus sp. strain 7 contains one [3Fe-4S] cluster (cluster I), one [4Fe-4S] cluster (cluster II), and one isolated zinc center. Oxidative degradation of this ferredoxin led to the formation of a stable intermediate with 1 zinc and approximately 6 iron atoms. The metal centers of this intermediate were analyzed by electron paramagnetic resonance (EPR), low temperature resonance Raman, x-ray absorption, and (1)H NMR spectroscopies. The spectroscopic data suggest that (i) cluster II was selectively converted to a cubane [3Fe-4S](1+) cluster in the intermediate, without forming a stable radical species, and that (ii) the local metric environments of cluster I and the isolated zinc site did not change significantly in the intermediate. It is concluded that the initial step of oxidative degradation of the archaeal zinc-containing ferredoxin is selective conversion of cluster II, generating a novel intermediate containing two [3Fe-4S] clusters and an isolated zinc center. At this stage, significant structural rearrangement of the protein does not occur. We propose a new scheme for oxidative degradation of dicluster ferredoxins in which each cluster converts in a stepwise manner, prior to apoprotein formation, and discuss its structural and evolutionary implications.Journal of Biological Chemistry 09/2000; 275(33):25391-401. DOI:10.1074/jbc.M909243199 · 4.60 Impact Factor
Article: The zinc metalloregulatory protein Synechococcus PCC7942 SmtB binds a single zinc ion per monomer with high affinity in a tetrahedral coordination geometry[Show abstract] [Hide abstract]
ABSTRACT: The Synechococcus PCC7942 SmtB is a zinc-responsive transcriptional repressor and a member of the ArsR superfamily of prokaryotic metalloregulatory transcription factors. The mechanism of negative regulation by Zn(II) and other metals as well as the coordination chemistry (stoichiometry, affinity, and specificity) of SmtB is poorly understood. In contrast to previous results [Kar, S. R., Adams, A. C., Lebowitz, J., Taylor, K. B., and Hall, L. M. (1997) Biochemistry 36, 15343-15348], we find that fully reduced SmtB binds 1 mol equiv of Zn(II) with a very high affinity, K(Zn) in excess of 10(11) M(-1) (pH 7.4, 0.15 M KCl, 22 degrees C). Optical spectroscopic experiments reveal that SmtB binds 1 mol equiv of Co(II) in a tetrahedral or distorted tetrahedral environment with one or two cysteine thiolate ligands in the first coordination shell. Zn(II) and Co(II) EXAFS studies are consistent with the optical spectroscopic data, and further suggest the presence of a mixture of carboxylate and imidazole-containing ligands. K(Co) was determined to be 1.7 (+/-0.1) x 10(9) M(-1) in a chelator (EGTA) competition assay; 1 equiv of Zn(II) results in complete displacement of the bound Co(II). SmtB also binds 1 mol equiv of Ni(II), which, when formed at low Ni(II):SmtB molar ratios, adopts a non-native, six-coordinate complex characterized by at least two histidine and no thiolate ligands. The hierarchy of metal binding affinities is Zn(II) > Co(II) > Ni(II).Biochemistry 09/2000; 39(38):11818-29. DOI:10.1021/bi001140o · 3.19 Impact Factor
Top Journals
Nippon Medical School
Tokyo, Tokyo-to, Japan
- Department of Biochemistry and Molecular Biology
Utah State University
Logan, OH, United States
- Department of Chemistry and Biochemistry
University of Wisconsin–Madison
Madison, Wisconsin, United States
- Department of Biochemistry
University of Georgia
Athens, GA, United States
- • Center for Metalloenzyme Studies
- • Department of Chemistry
Juntendo University
Edo, Tōkyō, Japan
- Department of Chemistry
Texas A&M University
College Station, TX, United States
- Department of Biochemistry/Biophysics


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Gideon Yu steps down as 49ers president
Gideon Yu, a former Facebook and YouTube executive credited with jump-starting the San Francisco 49ers' new stadium efforts, is stepping down as president of the club.
Yu announced the move Tuesday via his Twitter account.
"I'm stepping down as 49ers' President. Been an awesome ride. Will stay co-owner / #1 fan. Hope you love the new stadium!" he wrote.
Bizspace Spotlight
The team said it promoted executive vice president Paraag Marathe to team president. Al Guido, who oversaw club marketing, will serve as COO.
Yu joined the 49ers in 2011. He brought with him experience in finance that was key to helping the NFL club secure financing to build Levi's Stadium in Santa Clara. The 49ers in 2012 secured a $850 million loan from Goldman Sachs, US Bank and Bank of America to begin construction. The NFL agreed to provide $200 million. The club will get additional money from selling naming rights, seat licenses and from a city hotel tax.
The stadium is expected to open in time for the 2014 season. The stadium's overall cost is estimated at $1.2 billion.
Yu also contributed ideas on what the new 68,500-seat stadium should contain, such as a technology system that allows fans to use cell phones and other mobile devices during games.


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I can't remember an OS update or an iPhone update in which battery was not an issue. My battery life is better with iOS 6.1 and better than I got with the 4. Only difference might be that I do not leave LTE on- I use when speed is really needed.
2/8/13 at 2:54pm


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Select Local Merchants
Mona Lisa's Permanent MakeupRead MoreIf Ramona Lisa Abner ever goes to the Louvre, she'll probably get arrested for filling in the Mona Lisa's sparse brows. That's because Ramona?also known as Mona Lisa?embellishes facial features with permanent-makeup services. During each one-hour session, Mona imbues lips, lids, and brows with subtle shades that don't smudge after long days or encounters with moisture. She can also adorn cheeks with blush, camouflage irregular pigments, hide scars, and create Marilyn Monroe?style beauty marks. When she isn't sprucing up her clients, she hosts a five-day training course, an apprenticeship program, and a continuing-education class to help both new and experienced artists hone their permanent-makeup skills.20 High Street, Suite 126Hamilton, OH US
Paintball CountryRead MoreFlushed from his position, a player dashes from behind a tree and toward a bridge. Just as he reaches the opposite side, he hears "Dead man!" as an opponent pops up from underneath. Paintball Country's outdoor fields?which include an air ball field, six speedball fields, and five woods fields?present combatants with loads of such tactic-testing terrain. As referees maintain house rules to keep players safe, teams navigate obstacles such as two-story tower buildings, upturned cable spools, and corrugated tubing. Between firefights, Paintball Country's staff serves refreshments such as pizza, nachos, and victory-flavored energy drinks. An onsite pro shop also caters to guests with advice and equipment sales.5110 Lesourdsville West Chester Rd.Liberty Twp, OH US
jcpenney portraitsRead MoreWe are Erie's Premiere Senior portrait studio. If you are looking for cutting edge senior portraits, then you have found the right place!3459 Princeton RdHamilton, OH US
One Stop Tool RentalRead MoreOne Stop Rental accommodates customers with an invisible stockpile of efficient, clean burning propane. The non-toxic barbecue fuel not only helps to ensure safe outdoor cooking, but also produces less carbon dioxide than traditional grilling methods such as burning charcoal or searing steaks on the engine of a diesel truck. Though not included with today's Groupon, One Stop Rental can also help ignite backyard celebrations with propane grill rental and an assortment of party equipment.4182 Tonya TrlHamilton, OH US
La Crema Coffee CompanyRead MoreBlackberry tart. Banana-nut bread. Cinnamon-twist danish. They?re not decadent treats in a glass display case at the local bakery, but intoxicating aromas and robust flavors found at La Crema Coffee Company. Owned by Melissa and Dan Flohn, La Crema takes 100% Colombian coffee beans and transforms them into three dozen unique breakfast, afternoon, and dessert flavors such as chocolate raspberry, amaretto truffle, and bananas foster. For purists, the company imports unflavored blends from Central and South America, Indonesia, and Africa. It even vends a selection of non-coffee beverages, as well as hand-beaded coffee scoops and accessories for measuring coffee grounds or spooning them over breakfast cereal.9085 Sutton Pl.Hamilton, OH US
Offering's Holy Grounds CafeRead MoreWhether you prefer tea or coffee, Offering's Holy Grounds Cafe cafe has a wide selection of beverages. Score quick and easy seating for groups of any size at Offering's Holy Grounds Cafe. Not a popular place for dress-up dining, most Offering's Holy Grounds Cafe patrons come in casual attire. Call Offering's Holy Grounds Cafe for catering if you have a big event coming up. Patrons will love the number of street and lot parking options close to Offering's Holy Grounds Cafe. The food at Offering's Holy Grounds Cafe is not just delicious, it's also affordable.215 Main StHamilton, OH US


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Pool, workout room, staff was great in answering any questions we had.
Hard to get back on the NJ Parkway. Very slow wi-fi.
clean, nice, friendly staff.
No free breakfast
The room was clean . The hotel was clean.
Front desk agent had difficulty checking me in and she eas chewing gum.
Close to our destination
Hallways not air conditioned. Room had a slight 'locker room' scent. D
not a like to like about this place. for the price, the value is not there.
They used to have a free breakfeast. today it is $15.75. Are they really serious!
Clean rooms and staff was very friendly and helpful.
Awesome staff. Great pool, and breakfast
Friendly staff, nice pool.
The rooms are in need of remodeling and breakfast wasn't very good.
Great value for the cost. Not many dining options nearby, but Wawa across street offers a surprising variety of healthier grab-and-go options.
Price was reasonable and good location
Parking lot is very cramped
The location
The staff has such a strong accent I could barely understand what they were saying and the staff was not very polite. However, it could be due to a language barrier.
The location and the view were great. The pool was also nice.
We spent $600 for two nights at this hotel. A beach weekend for our family of four. When we checked in, they said we could park our car for the first night only (which we had to pay $20 for) but we'd have to move our car to street parking by noon the next day because there was a wedding and they wanted to reserve the onsite parking for wedding guests. That seemed crazy to me. The street meters had a 3-hour limit, so we spent our weekend walking back to the street every 3 hours to feed the meter. A good hotel doesn't do this to their guests.
The building looks pretty run down from the outside. It's old and there were some broken windows. Our room was fine, but the overall experience wasn't worth the money we spent. I'd look for something else next time.
Guadalupe at the front desk was awesome!
Room was comfortable and clean- bed and chair with ottoman
Loved the refrigerator and safe in room
Loved the shower set up totally cool!
Loved the shower gei, shampoo. Body lotion, mouth wash and soaps
Loved rhe coffee maker
I'vei was surprised that the interior of the hotel although clean wasn't more modernized. I knew that I was staying at anRadisson Hiotel but looking around as one walks through the hallways to the elevator you don't get that Radissom vibe The rates are too expensive.
Our sleep number bet didn't work with 2 people in it. Very uncomfortable
The Place is clean and the staff is friendly when you check in.
The rooms are nice and the beds are of good size.
It's close to a lot of local Toms River attractions and not far from the beach.
They do not have enough carts or anyone to help you with your bags.
Our Room was supposed to have a fridge it did not.
Hotel staff is friendly but it stops there no real help your on your own.
Side door to enter seems to be broken for weeks as we had to go through the front entrance and walk around the building which is a hassle when you have nobody to help with bags or enough carts.
No water in rooms you have to bring your own or use vending machine.
Staff helpful and went out of way to help. Convenient to everything. Rooms were clean and neat. Pool was wonderful to use in February with 2 grandchildren. Family and business friendly.
While we were there they had a problem with wifi . Resolved but count on internet.
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Reference | SO WHAT THE HECK IS THAT Jun 18, 2009
by Alice Gordenker
Dear Alice, Based on my forays into Japanese restaurants in North America, I was under the impression that Japanese cuisine didn't feature any spicy flavorsat all. Then, on my first trip to Japan, I wandered into a restaurant that specializes in soba noodles. When ...


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Food and Staples Retailing
Company Overview of A & B Produce, Inc.
Company Overview
A & B Produce, Inc. sells a range of produce, dairy, and food items to restaurants and dining facilities in the San Francisco Bay Area. It sells beans and rice, dried chili, dry goods, eggs, onions and garlic, flowers and leafs, lettuces, organics, prepared items, potatoes, vegetables, tortillas, and other specialty items. The company was founded in 1972 and is based in South San Francisco, California. A & B Produce, Inc. operates as a subsidiary of Bi-Rite Restaurant Supply Co., Inc.
299 Lawrence Avenue
South San Francisco, CA 94080-6526
United States
Founded in 1972
Key Executives for A & B Produce, Inc.
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78125 Cabrillo Lane 25 in Indian Wells, CA
This delightful lower unit has two sliders opening out to the large wrap-around patio. This condo is nicely decorated in pleasant colors and is offered TURNKEY furnished...just bring your suitcase. Dining area wall is mirrored which enhances the size of the living area and the brightness of the condo because of the numerous windows. There are (2) master suites with private baths. One of the bedrooms has a King bed and the other a Queen bed. Both suites have full baths. There is also a half bath near the kitchen. One of the bedroom suites can be closed off and has its own private entry. The association pool is just steps from the condo, as is the association laundry. Mountain Cove is a guard gated community adjacent to Indian Wells C.C., nestled against the mountains away from the wind. Available Nov-Apr $2500/mo...utilities with cap included; May-Oct $1100/mo. PLUS Electricity.Check Availability
78125 Cabrillo Lane 2578125 Cabrillo Lane 25, Indian Wells, CA 92210 USA
Floor Plans and Pricing
2 Bedrooms
|78125 Cabrillo Lane 25
|2.5 Bathrooms
|1152 Sq. Ft.
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- Application Fee:
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- Deposit:
Property Features
- Community Features
Community Features
- House for Rent
Additional Information
Year Built: 1972
Apartments in Community: 1


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Product Information
Farewell! Old Cottage composed by Stephen C. Foster - Digital Sheet Music
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Farewell! Old Cottage digital sheet music. Contains printable sheet music plus an interactive, downloadable digital sheet music file.
* This arrangement contains partial lyrics
* This product is available worldwide
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Finding your center means settling into a stable, coherent state of awareness. Outer forces do not dominate you.
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