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David West on Roy Hibbert: 'He's got to be part of the fight'
More postseason coverage: Playoff schedule, results | Latest news, notes
Roy Hibbert didn't score a single point on Monday. It was his third scoreless outing in eight playoff games. The 7'2, 290-pound Indiana Pacers center didn't grab a rebound, either. In 18 minutes against the Washington Wizards, he collected five fouls, shot 0-for-2 from the field and the Pacers were outscored by 17 points.
Some thought the matchup with Washington would change Hibbert's poor postseason fortunes, but, at least after Game 1, that doesn't appear to be the case. In a postgame scrum following Indiana's 102-96 loss, forward David West strongly spoke up about his frontcourt partner.
A beyond furious David West, on Hibbert: "He's got to be part of the fight. H's got to be part of this thing for us to go anywhere."— Scott Agness (@ScottAgness) May 6, 2014
Hibbert is averaging 4.6 points and 3.3 rebounds in 21 minutes per game in the playoffs, shooting 36 percent from the field. The big man ended the regular season in a serious slump, but this is a new low. Forward Paul George echoed West in saying that Hibbert must contribute if the Pacer are going to get where they want to go.
Paul George on Hibbert: "We're at the point where we really need Roy and we need him now."— Candace Buckner (@CandaceDBuckner) May 6, 2014
PG: "(It) comes down to us having heart and rebounding the ball. We need our big fella to rebound the ball for us…"— Candace Buckner (@CandaceDBuckner) May 6, 2014
West, Hibbert and veteran forward Rasual Butler reportedly had a private talk in the weight room, but declined to comment on it, via Scott Agness of Pacers.com.
Hibbert himself acknowledged that things needed to change.
Roy Hibbert: "I'm going to change some things up for the second game. I'm going to look within myself and go out there and figure it out."— Scott Agness (@ScottAgness) May 6, 2014
Hibbert: "I got to come out and be aggressive. I got to be a different Roy Hibbert than I have been."— Scott Agness (@ScottAgness) May 6, 2014
The increasingly concerning issue is that this version of Hibbert has become the norm. His 13-point, seven-rebound, five-block performance in Game 7 against the Atlanta Hawks was the only time he'd reached double figures in the last month. He clearly didn't fit against a Hawks team with numerous three-point-shooting bigs, but the Wizards don't pose the same problem.
Washington is a solid team, though, and it has proven capable of creating good looks against the Chicago Bulls' stingy defense. Indiana will have to be much better than it was against Atlanta in order to advance past the second round. It's hard to imagine it happening without Hibbert playing a bigger part.


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The contribution of oxidative stress to diabetic complications including neuropathy is widely known. Mitochondrial and cellular damage are associated with the overproduction of reactive oxygen species and decreased levels or function of the cellular antioxidant mitochondrial manganese superoxide dismutase (SOD2). We hypothesized that targeted SOD2 deletion in the peripheral nervous system using cre-lox technology under control of the nestin promoter would accelerate neuropathy in a type 2 model of diabetes, the BKS.db/db mouse. SOD2-deficient mice, however, demonstrated severe gait deformities and seizures and died by 20 days of age. Examination of SOD2 expression levels revealed that SOD2 was lost in brain and reduced in the spinal cord, but appeared normal in dorsal root ganglia and peripheral nerves in SOD2-deficient mice. These findings indicate incomplete targeted knockout of SOD2. Morphological examination revealed cortical lesions similar to spongiform encephalopathy in the brain of SOD2-deficient mice. No lesions were evident in the spinal cord, but changes in myelin within the sciatic and sural nerves including a lack of cohesion between layers of compact myelin was observed. Together, these results indicate that targeted neuronal SOD2 knockout using the nestin promoter results in severe central nervous system degeneration and perinatal lethality in mice. A specific peripheral nervous system-targeting construct is required to examine the consequences of SOD2 knockout in diabetic neuropathy.
Oxidative stress and mitochondrial dysfunction are implicated in the pathogenesis of multiple diseases of the central and peripheral nervous systems, including Parkinson's and Huntington's disease, amyotrophic lateral sclerosis, nervous system aging and diabetic neuropathy (DN). Substantial evidence implicates impaired mitochondrial energy production and increased mitochondrial oxidative damage early in the pathology of neurodegeneration (Moreira et al., 2010). Normally, cellular respiration results in the production of reactive oxygen species (ROS) that are neutralized by mitochondrial manganese superoxide dismutase (MnSOD or SOD2). In disease states, however, changes in mitochondrial respiration or a decreased cellular antioxidant capacity results in increased ROS levels and subsequent mitochondrial and cellular damage (Leinninger et al., 2006, Vincent et al., 2007, Naoi et al., 2009, Schon and Przedborski, 2011). It remains unclear, however, whether oxidative stress is the primary defect in the pathology of these diseases or a result of cellular dysfunction associated with neurodegenerative disease (Andersen, 2004).
The pathological consequences of increased ROS accumulation has previously been examined using mice with inactive or deleted SOD2 (Vincent et al., 2007). Complete loss of SOD2 in mice, however, results in embryonic or early postnatal lethality. This has been observed across genetic backgrounds, including CD-1 (Li et al., 1995), C57BL/6 (Ikegami et al., 2002) and DBA/2J (Huang et al., 2001), and is most likely due to cardiomyopathy. SOD2+/− heterozygous mice which have reduced SOD2 expression levels, on the other hand, have a normal lifespan, indicating that under normal conditions these mice are relatively healthy; however, they do exhibit an increased incidence of tumors (Elchuri et al., 2005) and significantly elevated measures of oxidative stress. Therefore, we previously examined the development of DN in diabetic C57Bl/6 SOD2+/− mice (Vincent et al., 2007). While no significant neuropathy was observed in a type 1 diabetes model, type 2 diabetic C57Bl/6.db/db SOD2+/− mice exhibited increased manifestations of DN compared to C57Bl/6.db/db SOD2+/+ control mice, suggesting that oxidative stress is important in the pathogenesis of DN (Vincent et al., 2007).
In the current study, we were interested in examining the specific effects of oxidative stress in neurons on DN onset and progression in BKS.db/db mice, a spontaneous and well-established model of type 2 diabetes which exhibits diabetes onset around 4 weeks of age and neurological deficits after 8 weeks (Sullivan et al., 2007). To circumvent the embryonic lethal effects of complete SOD2 deficiency, we utilized cre-lox technology to generate nervous system-specific SOD2 knockout BKS.db/db mice. We discovered, however, that targeted neuronal deletion of SOD2 under the nestin promoter is perinatally lethal and animals only survived for up to 3 weeks. Given the standard onset of diabetes and DN in BKS.db.db mice of 4 and 8 weeks, respectively, this prohibits any conclusions regarding the role of oxidative stress on the development of DN in these mice. Therefore, we performed a comprehensive morphological assessment to gain further insight into the effects of increased ROS in the central and peripheral nervous systems. We observed complete SOD2 deficiency in the brain of these mice, with select regions demonstrating increased oxidative stress and resulting pathology. SOD2 expression was also lost in a subset of motor neurons in the spinal cord and reduced in Schwann cells, but no major impact on SOD2 expression was seen in dorsal root ganglion neurons. These tissues were further characterized for physiological and morphological features, and our findings suggest that targeted deletion of SOD2 results in severe central nervous system degeneration characterized by mitochondrial damage within the brain and seizures that lead to perinatal lethality. Future studies examining the contribution of increased ROS to DN require a more specific targeting construct to delete SOD2 in the peripheral nervous system and circumvent the early lethality of central nervous system SOD2 deletion in BKS.db/db mice.
In collaboration with Jackson Laboratories (Bar Harbor, Maine), cre-lox technology was used to generate nervous system-specific SOD2 knockout mouse lines on a diabetic background. Cre expression was driven by the nestin promoter in B6.Cg-Tg(Nes-cre)1Kln/J mice (nes-cre+ or nes-cre−) to knock out floxed SOD2 (SOD2fl/fl or SOD2fl/+) in neuronal tissues (Tronche et al., 1999) of mice on the BKS.Leprdb/db background (BKS.db/db, BKS.db+ or BKS.+/+). To verify the genotype of all mice, genomic DNA preparation and PCR genotyping were performed using the Jackson Laboratory protocol (Bar Harbor, ME). Breeding colonies consisting of BKS.db+ SOD2fl/fl mice and BKS.db+ SOD2fl/+ nes-cre+/− mice were established at the University of Michigan to provide the animals used in this study. All animals were cared for following the University of Michigan Committee on the Care and Use of Animals guidelines.
The following groups were planned for our initial analysis: BKS.db/db SOD2fl/fl cre+, BKS.db/db SOD2fl/fl cre−, BKS.db+ SOD2fl/fl cre+ and BKS.db+ SOD2fl/fl cre−. Surprisingly, genotyping at weaning revealed no pups expressing the SOD2fl/fl cre+ genotype regardless of leptin receptor mutation status. These findings lead us to suspect a perinatal lethal effect of the targeted SOD2 deletion; therefore, subsequent litters were genotyped and checked daily from birth. Genotyping confirmed that pups dying between birth and weaning age were SOD2fl/fl cre+. Due to the early lethality of the SOD2fl/fl cre+ phenotype, and the fact that diabetes onset in BKS.db/db mice occurs around 4 weeks of age (Sullivan et al., 2007), we revised our experimental paradigm to investigate the impact of neuronal-specific SOD2 deficiency in these mice. Initial findings using hematoxylin & eosin staining (Carson and Hladik, 2009) to examine general histology revealed no gross anatomical abnormalities in liver, cardiac or skeletal muscle and no detectable differences in survival or neuronal pathology between BKS.db/db, BKS.db+ or BKS.+/+ mice (data not shown); therefore, for all subsequent studies, SOD2fl/fl or SOD2-deficient refers to BKS.+/+ SOD2fl/fl nes-cre+ mice expressing the targeted deletion of SOD2, and SOD2fl/+ refers to BKS.+/+ SOD2fl/+ nes-cre+ mice that express normal SOD2 levels.
SOD2-deficient pups became moribund between postnatal days 15–20; therefore, moribund pups and healthy littermate controls were euthanized at 10–14 d for tissue collection and morphological analyses. For general morphological examination and analysis of SOD2 expression levels, mice were euthanized by CO2 inhalation and brain, spinal cord, dorsal root ganglia (DRG), sciatic and sural nerves, liver, skeletal muscle and heart were dissected and immediately immersed in 10% neutral buffered formalin (Fisher) for histological studies or snap frozen in liquid nitrogen for storage at −80 C until needed for immunoblotting. For morphology studies, tissues were processed on a Tissue Tek VIP processor (Sakura Finetek), dehydrated and embedded in paraffin for sectioning. Sections were processed for standard hematoxylin and eosin staining or bright field immunohistochemistry (IHC) (Carson and Hladik, 2009). Briefly, IHC was performed using a Biocare automated stainer. Sections were treated with an antigen deblocker (RD913, Biocare) followed by peroxidase for 5 min. A rodent blocking solution (RBM961, Biocare) was applied for 30 min and sections were incubated with antibodies against GFAP (1:1000, ab7260, Abcam), neurofilament (NF; 1:500, ab40796, Abcam) and a marker of microglia (1:300, CP290, Biocare). Secondary rabbit anti-mouse horseradish peroxidase (HRP) was applied for 30 min and staining was revealed with 3′3-diaminobenzidine (DAB). Sections were counterstained with hematoxylin.
For immunoblotting, tissue lysates were prepared as previously described (Kim et al., 2009). The lysates were separated by SDS-PAGE and transferred to nitrocellulose membranes for incubation with the desired primary and appropriate HRP-conjugated secondary antibodies. Antibodies against SOD2 (1:1000, ADISOD110, Enzo Life Sciences), Actin (1:1000, ab8227, Abcam) and GAPDH (1:1000, MAB374, Millipore) were used. The signal was visualized using enhanced chemiluminescence reagents (ECL; Amersham Bioscience, Piscataway, NJ) and images were captured using the Chemidoc XRS system (BioRad). Denstiometric analysis was performed using Quantity One software (BioRad).
Alternatively, for immunofluorescence (IF), mice were anesthetized by intraperitoneal injection of sodium pentobarbital (Fatal-plus; Dearborn, MI) and perfused through the left ventricle with phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (PFA) in 0.1M phosphate buffer (PB, pH 7.4). Dissected brain, spinal cord and DRG, were fixed with 4% PFA, gradually cryoprotected in PB containing sucrose (10, 20, and 30%) and embedded for cryosectioning as previously described (Sullivan and Feldman, 1994, Cheng et al., 1996). Frozen sections were incubated with antibodies against SOD2 (1:200, ADISOD110, Enzo Life Sciences), NF (1:250, MAB5256X, Millipore), choline acetyltransferase (ChAT; 1:250, AB144P, Millipore) to identify motor neurons, and pyruvate dehydrogenase complex (PDH; 1:100, MSP06, Mitosciences) to label mitochondria, using a Mouse-On-Mouse kit (FMK2201, Vector). Sections were counterstained with fluorescent Nissl (1:50, N21480, Invitrogen). Motor neuron numbers in cervical and lumbar spinal cord sections were determined from every fourth 16 μm serial section for a total 10 sections each.
Brain, brain stem and sciatic and sural nerves were immersion fixed with 2.5 percent glutaraldehyde in 0.1 M Sorensen's buffer, pH 7.4. After several buffer rinses, tissues were immersed for 1 h in 1% osmium tetroxide in the same buffer. To remove phosphate, double distilled water was used followed by en bloc staining with aqueous 3% uranyl acetate for 1 h. Tissues were then dehydrated in ascending concentrations of ethanol, transitioned through propylene oxide, and embedded in Epon epoxy resin (Edwards et al., 2010). Semi-thin sections were stained with toluidine blue for tissue orientation. Cross-sectional areas were ultra-thin sectioned (70 nm) and stained with uranyl acetate and lead citrate. They were examined using a Philips CM100 electron microscope at 60 kV. Images were recorded digitally using a Hamamatsu ORCA-HR camera system operated using AMT software (Advanced Microscopy Techniques Corp., Danvers, MA).
SOD2-deficient pups developed an abnormal gait, had difficulty righting themselves between postnatal days 10–14 and became moribund between postnatal days 15–20. Genotyping confirmed that pups dying between birth and weaning age were SOD2fl/fl and cre+. These pups were small compared to their littermates; at 20 days of age the body weight of SOD2fl/fl pups averaged 3.51 g, compared to an average of 7.5 g for SOD2fl/+ pups.
Previous observations of animals in which SOD2 was globally deleted revealed severe cardiac myopathy, lipid accumulation in muscle and liver and metabolic acidosis (Li et al., 1995); therefore, cardiac muscle, skeletal muscle and liver paraffin sections were stained with hematoxylin and eosin to examine the general effects of targeted SOD2 deletion. Hearts from SOD2fl/fl and SOD2fl/+ mice both exhibit a normal ventricular size and wall thickness, and cardiac muscle histology is normal with no evidence of degeneration, atrophy or hypertrophy (data not shown). SOD2fl/fl and SOD2fl/+ mice also demonstrate normal skeletal muscle histology, and liver from both groups was normal with no evidence of steatosis (data not shown). Overall, no abnormalities in cardiac and skeletal muscle or liver in SOD2fl/fl and SOD2fl/+ mice were revealed.
SOD2 expression levels were examined by western immunoblotting in brain, cardiac muscle, skeletal muscle and liver, and confirmed that SOD2 knockdown was restricted to nervous tissue in these mice (Figure 1A). However, further examination of neuronal tissues including DRG, cervical and lumbar spinal cord sections and sciatic nerve by western immunoblotting revealed variable effects of SOD2 knockdown in these tissues (Figure 1B). Both cervical and lumbar spinal cord tissue exhibited a considerable decrease in SOD2 levels in SOD2-deficient mice, whereas sciatic nerve tissue exhibited a slight decrease in SOD2 expression levels in SOD2-dificient mice. Little to no effects on SOD2 levels were observed in DRG. Together, these data reveal that SOD2 knockdown is inconsistent across neuronal tissues, thus supporting further investigation to identify the cell types affected and determine the extent and consequences of nestin-induced SOD2 knockdown in the central and peripheral nervous systems.
At approximately 10–15 days of age, SOD2fl/fl mice develop severe seizures and motor deficits; therefore, we first examined pathology within the central nervous system. Hematoxylin and eosin staining of brains from SOD2-deficient mice revealed varying degrees of vacuolization within the cortical neuropil (Figure 2). These bilateral symmetrical cortical lesions were detected in all SOD2fl/fl mice (Figure 2B, D) and never in the SOD2fl/+ mice (Figure 2A, C). Bilateral vacuolar lesions were also present in the hippocampus, thalamus and brain stem (data not shown). At the level of light microscopy, the vacuoles appear extra-neuronal and were often surrounded by compressed but intact neurons. SOD2 IF was localized within neurons and the neuropil in the SOD2fl/+ mice, but was absent in all cell types of the SOD2fl/fl mice (Figure 2E, F), although on very rare occasions, a SOD2-positive neuron was present.
Further examination of the cortex from SOD2fl/fl (Figure 3B, D, F) and SOD2fl/+ (Figure 3A, C, E) mice included IHC for NF, the astrocyte marker GFAP and the microglial marker CP290. Intact cortical neurons surrounding many of the vacuoles are apparent in the cortex of SOD2fl/fl mice, but these vacuoles do not appear to be internal to any cortical neurons (Figure 3A, B). Similarly, numerous astrocytes with normal morphology are observed immediately adjacent to the vacuoles, with some cortical regions in the SOD2fl/fl mice exhibiting a trend for slightly increased astrocyte numbers (Figure 3C, D). Microglial staining was not indicative of any inflammatory changes associated with vacuolar lesions (Figure 3E, F).
To determine whether the vacuoles were truly extracellular, we examined the cortex of SOD2fl/fl and SOD2fl/+ mice using TEM (Figure 4) which revealed both extra- and intracellular changes in the SOD2fl/fl mouse cortex (Figure 4D–I). Consistent with our observations using NF IHC (Figure 3), many neurons were compressed by extracellular vacuoles. Some vacuoles contained what appeared to be the membranous remnants of cellular organelles (Figure 4G–I; arrowheads), indicating that they could be representative of ruptured cells, as they were occasionally surrounded by small myelinated fibers. Intraneuronal changes included disrupted endoplasmic reticulum and swollen mitochondria with disrupted or missing cristae (Figure 4D–F; arrows). Apoptotic ultrastructure including nuclear condensation and blebbing was only rarely detected (data not shown). Similar lesions were observed in the hippocampus, thalamus and brain stem, and no abnormal pathologies were detected in astrocytes (data not shown). Overall, analysis of the central nervous system in SOD2fl/fl mice revealed cortical vacuolization and mitochondrial disruption.
Cortical pathology has been reported in SOD2 knockout animal models; however, none of these reports examined the spinal cord or any aspect of the peripheral nervous system; therefore, we next examined SOD2 expression and PDH-labeling of mitochondria in the cervical and lumbar spinal cord (Figure 5). Neither motor neuron loss nor vacuolar changes were observed in the cervical or lumbar spinal cord of the SOD2fl/fl mice (Figure 5D–F, J–L) or the SOD2fl/+ mice (Figure 5A–C, G–I). SOD2 expression was lost in a number of motor neurons in the ventral horn region (Figure 5F, L); however, ChAT co-localization clearly depicts a subpopulation of SOD2-positive motor neurons (Figure 5F, L). While there was no apparent decrease in the total number of motor neurons, the number of SOD2-positive cells decreased in spinal cord. We also detected a general trend for greater numbers of SOD2-positive neurons within the cervical spinal cord (Figure 5E) compared to the lumbar spinal cord (Figure 5K); 33.7% of the total motor neurons in the cervical spinal cord and 11.2% of total motor neurons in the lumbar spinal cord were SOD2-positive in SOD2fl/fl mice. Motor neurons that did not express SOD2 had no obvious pathology and appeared normal.
We next examined the effects of SOD2 expression levels on mitochondria in motor neurons in the lumbar spinal cord. The overall level of PDH IF appears slightly reduced in SOD2fl/fl mice compared to SOD2fl/+ mice, but there were no detectable differences in the distribution of mitochondria in the ventral horn (Figure 6A, D). In the dorsal horn, generally lower levels of SOD2 and PDH IF were detected within neurons and the neuropil, however, no significant differences between the SOD2fl/fl and SOD2fl/+ mice were identified (data not shown). Together, these data suggest that there is no significant neuronal pathology within the spinal cord of SOD2fl/fl mice, and indicate that SOD2 knockdown is not complete in the motor neuron population.
DN involves the degeneration and loss of sensory neurons of the peripheral nervous system; therefore, we next examined the impact of nes-cre-induced SOD2 knockout on DRG and peripheral nerves. Hematoxylin and eosin staining revealed no differences in DRG neuronal morphology between SOD2fl/fl and SOD2fl/+ mice (data not shown). SOD2 IF was similar between groups, and appeared as evenly distributed punctate fluorescence throughout the cytoplasm (Figure 7B, C, E, F). We did not observe any significant differences in the amount or distribution of mitochondrial PDH IF between SOD2fl/fl and SOD2fl/+ mice (Figure 7A, D). Differences were apparent, however, when the fluorescent signals were combined; SOD2fl/+ mice exhibited substantial overlap of SOD2 and PDH fluorescence, while the SOD2-deficient mice exhibited considerably less co-localization (Figure 7C, F).
We next examined sciatic and sural nerves. No significant differences in axonal structure or general fiber density were observed between SOD2fl/fl and SOD2fl/+ mice (data not shown). Both PDH and SOD2 IF were prominent in Schwann cell cytoplasm but were not detected within axons (data not shown). TEM of SOD2fl/fl mouse sciatic (Figure 8A–B) and sural (Figure 8C–D) nerves, however, revealed prominent changes including myelin delamination and disrupted mitochondria in myelinating Schwann cells. Few changes were detected within non-myelinating Schwann cells or in myelinated or unmyelinated axons. These data indicate that nes-cre expression does not result in complete knockout SOD2 expression in DRG, but examination of peripheral nerves reveals defects in myelination and in SOD2 expression in Schwann cells.
Oxidative damage disrupts multiple cellular processes from transcription and translation to mitochondrial function and axonal transport, and our laboratory has been instrumental in establishing the impact of oxidative stress on the development and progression of DN (Russell et al., 2002, Feldman, 2003, Vincent et al., 2004, Vincent et al., 2005, Vincent et al., 2007, Russell et al., 2008). Recently, we reported that elevated triglycerides correlate with DN progression in human patients with diabetes (Wiggin et al., 2009) and that oxidized lipids are toxic to sensory neurons (Vincent et al., 2009). Therefore, the current study was aimed at investigating increased oxidative stress in the context of type 2 diabetes, a condition characterized by hyperglycemia and dyslipidemia. Cre-lox technology was used to create a targeted nervous system knockout of the mitochondrial detoxifying enzyme SOD2 in BKS.db/db mice, a robust model of type 2 diabetes. Targeted knockout of SOD2, however, resulted in a spongiform pathology in the brain and was perinatally lethal, independent of the leptin receptor mutation. In addition to the effects of SOD2-knockout in the brain, we also report differences in SOD2 expression in the spinal cord and peripheral nervous system; to our knowledge this is the first description of these changes. Overall, these studies validate the link between oxidative stress and neurodegeneration, and provide novel insight into the role of SOD2 in various neuronal tissues.
SOD2 is an essential component of the cellular antioxidant defense against superoxide accumulation (Moreira et al., 2010), and numerous studies have altered SOD2 expression in animal models to investigate the role of oxidative stress on various tissues. Early studies examining whole-animal inactivation of SOD2 found that loss of SOD2 function resulted in perinatal lethality around P10 (Li et al., 1995, Melov et al., 1998). Brain histology was normal and no neurological phenotype was observed; therefore, the authors concluded that the mice died from cardiac myopathy resulting from oxidative stress and abnormal mitochondria within cardiac myocytes (Li et al., 1995). Since that time, whole-animal SOD2 deletion has also been examined on various genetic backgrounds (Huang et al., 2001). While SOD2 deletion in C57Bl/6 mice results in embryonic lethality (E15) due to cardiomyopathy, DBA/2J and B6D2F1 mice survived to 12 days and 3 weeks, respectively, and did not exhibit a cardiac phenotype – these mice likely died from metabolic acidosis, indicative of overall reduced antioxidant defense (Huang et al., 2001). Careful examination of SOD2 knockout mice revealed that oxidative phosphorylation markers are reduced in multiple tissues following whole-animal SOD2 deletion. Specifically, complex II and III in skeletal and cardiac muscle and citrate synthase in cardiac muscle were significantly decreased, as was aconitase in cardiac muscle and brain (Melov et al., 1999). These tissues all exhibited increased levels of oxidative stress.
The ability to detoxify ROS is of particular importance in any long-lived, non-mitotic cells, including neurons. Therefore, we were interested in investigating the role of ROS on neurodegeneration in DN. In collaboration with Jackson Laboratories, we developed a nervous system-specific SOD2-deficient mouse using cre-lox technology, with cre expression driven by the nestin promoter to drive expression in developing neuronal cells (Tronche et al., 1999). Our initial observations revealed that no SOD2fl/fl pups survived to weaning age; the animals developed motor abnormalities between 10–14 days and show seizures with death likely resulting from cardiac arrest and/or arrhythmias secondary to continuous convulsions at 3 weeks of age. General pathological findings and western immunoblotting confirmed that SOD2 deletion was confined to the nervous system in these mice (Figure 1), indicating that oxidative stress in other tissues was not a contributing factor to lethality. Furthermore, we detected no effect of the leptin receptor mutation on survival, SOD2 expression, or nervous system pathology at this age. Because diabetes and DN onset in BKS.db/db mice does not occur until well after SOD2-deficient mice died, we chose to perform a comprehensive analysis of neuronal tissues in two groups of mice for the current studies: SOD2fl/fl and SOD2fl/+ mice on the BKS.+/+ background.
Severe central nervous system defects were observed in SOD2fl/fl mice. Spongiform vacuolization and mitochondrial damage were evident in multiple regions, including layers 3–6 of the cortex, the hippocampus, thalamus and brain stem (Figures 2–4, data not shown). These spongiform changes are consistent with those previously described in the cortex and brainstem of complete SOD2 knockout animals (Melov et al., 1998). The majority of the damage appears to be extracellular in that it is not localized near neuronal nuclei; however, due to the appearance of organelle remnants, it is possible that the vacuoles represent ruptured neurons or cellular areas beyond the nucleus. No significant damage to the surrounding neuropil, including profiles of passing axons and dendrites or in astrocytes and microglia was found, and no degenerative changes were observed in the white matter. Examination of astrocytes and microglia revealed normal morphology and distribution indicating that these cells were not affected by SOD2 deletion at the time of death, although we observed a trend towards a slight increase in astrocyte numbers in SOD2fl/fl mouse brains. This is not surprising, given the degenerative changes observed in the cortex. Furthermore, these findings are also supported by previous reports of mostly normal glia and no changes in white matter morphology (Melov et al., 1998), and the identification of prominent changes in the M1 and M2 regions of the cortex and within the motor nucleus of the trigeminal nucleus (Melov et al., 2001). Together, these data support the conclusion that more metabolically active neurons are affected by SOD2 deletion, and it is possible that glial cell types would be equally affected if stressed.
Previous studies did not address the effects of SOD2 knockout on the spinal cord or peripheral nervous system; therefore, we examined the DRG, peripheral nerves and cervical and lumbar regions of the spinal cord in SOD2fl/fl and SOD2fl/+ mice. Basic histology generally appeared normal in the targeted SOD2fl/fl mice, and spongiform or degenerative lesions were not detected in any part of the DRG, peripheral nerves or spinal cord gray or white matter. These limited pathological findings in SOD2fl/fl mice reflect the fact that SOD2 knockout was incomplete in neuronal tissues outside of the brain. In the DRG (Figures 1B, ,7),7), we detected no significant changes in the levels or localization of SOD2 in the SOD2fl/fl mice. Within peripheral nerve (Figures 1B, ,8),8), SOD2 immunoreactivity was evident in the cytoplasm of Schwann cells of SOD2fl/fl mice and appeared moderately diminished compared to controls. Examination of peripheral nerve ultrastructure revealed disrupted mitochondria in myelinating Schwann cells, while non-myelinating Schwann cells were normal. Axonal mitochondria were also normal. Due to the early lethality of the targeted SOD2 deletion, we were not able to assess nerve conduction velocities for either motor or sensory modalities.
In the spinal cord, SOD2 expression markedly decreased, but was still evident in a subset of motor neurons in SOD2fl/fl mice (Figures 1B, ,5,5, ,6).6). Given the extent of SOD2 knockdown, however, significant repercussions on the motor neuron microenvironment are possible despite the incomplete nature of SOD2 knockout within that cellular population. We also observed a trend for increased levels of SOD2 expression and increased numbers of SOD2-expressing motor neurons within the cervical spinal cord compared to the lumbar spinal cord. These motor neurons within the ventral horn displayed normal morphology, and examination of mitochondrial PDH IF in the spinal cord demonstrated co-localization with SOD2 in the SOD2fl/+ mice, but an apparent lack of co-localization in the SOD2fl/fl mice. Previous attempts to investigate targeted SOD2 knockout in motor neurons revealed similar findings and exhibited incomplete SOD2 deletion in motor neurons (Misawa et al., 2006). These animals exhibited a normal lifespan, displayed no motor weakness, tremors or paralysis and maintained normal neuronal numbers. Motor neurons within brain stem and ventral spinal cord, however, did exhibit increased oxidative stress and accelerated Wallerian degeneration following axotomy (Misawa et al., 2006). A role for oxidative damage and mitochondrial dysfunction in motor neurons has also been previously examined by expressing mutated copper-zinc superoxide dismutase (SOD1) in SOD2-deficient mice (Andreassen et al., 2000). SOD1 mutations are implicated in the pathology of familial amyotrophic lateral sclerosis. These mutations do not affect the dismutase activity of SOD1 and neuronal death is likely due to a toxic gain of function (Ilieva et al., 2009). Expression of SOD1G93A in SOD2-deficient mice resulted in a reduced lifespan and earlier onset of motor neuron death compared to SOD1G93A mice without a SOD2 deficiency (Andreassen et al., 2000). Together, these findings support a connection between oxidative stress, mitochondrial dysfunction and neurodegeneration, but alternative approaches are required to investigate the consequences of SOD2 loss on the peripheral nervous system.
The cre-lox system provides an enticing approach to investigate the effects of tissue-specific targeted knockout of genes (Ray and Gage, 1992, Ray et al., 2000, Yu and Bradley, 2001), and multiple groups have used this technology in various tissues in an attempt to circumvent the early lethality of complete SOD2 knockout in mice (Ikegami et al., 2002, Misawa et al., 2006, Nojiri et al., 2006, Kuwahara et al., 2010, Shimizu et al., 2010, Lustgarten et al., 2011, Parajuli et al., 2011). SOD2 deletion in kidney, skeletal muscle and liver all induced increases in oxidative stress measures, but no drastic effects on the specific tissues or lifespan was observed (Ikegami et al., 2002, Kuwahara et al., 2010, Lustgarten et al., 2011, Parajuli et al., 2011). For the current studies, we chose to combine cre-lox technology with the nestin promoter for its ubiquitous expression within the developing nervous system (Dahlstrand et al., 1995, Lothian and Lendahl, 1997, Guo et al., 2005, Yu et al., 2005). At the time we began these experiments, no central or peripheral nervous system-specific SOD2 deletion had been reported. Since that time, targeted deletion of SOD2 in the spinal cord and brain stem has been reported, although these animals exhibit incomplete SOD2 knockout, a normal lifespan and remarkably, no motor deficits or neuronal loss (Misawa et al., 2006). Central nervous system SOD2 deletion has also been briefly examined, and similar to our findings, mice exhibit seizures and die at approximately 3 weeks of age (Shimizu et al., 2010); however, a thorough characterization of the model was not described. Neurons from these mice have been used to examine the contribution of SOD1 and SOD2 to oxidative damage following hypoxia and reoxygenation (Sasaki et al., 2011). Taken together, these data indicate that tissue-specific knockout of SOD2 does increase oxidative stress and elicits subsequent tissue damage, but alone is not enough to recapitulate specific disease phenotypes. It is possible that compensatory mechanisms may be involved, although SOD1 mRNA, protein or enzyme activity were not upregulated in many of these models where mechanistic studies were investigated (Saito et al., 1995, Rebelo et al., 2007, Kuwahara et al., 2010, Shimizu et al., 2010, Parajuli et al., 2011); this remains an area of intense investigation.
To investigate the impact of ROS and mitochondrial dysfunction on DN, and establish models for future therapeutic development studies, a more restricted and specific promoter to target SOD2 deletion to sensory neurons is required. There are a number of neural crest-specific promoters; however, loss of sympathetic innervation and loss of the enteric nervous system would complicate data interpretation due to effects on vascular tone and nutrition. DRG11 and Foxs1 are specifically expressed in developing sensory neurons (Saito et al., 1995, Montelius et al., 2007, Rebelo et al., 2007), with DRG11 preferentially expressed in small DRG neurons (Saito et al., 1995, Rebelo et al., 2007). Either of these promoters in combination with cre-lox technology would be useful in targeting all or a subset of DRG neurons in future experiments
Overall, the current study demonstrates that targeted deletion of SOD2 using the nestin promoter results in mitochondrial damage within the brain and seizures that lead to perinatal lethality. While the incomplete loss of SOD2 expression in peripheral nervous tissues and the early death of these animals precludes an in-depth analysis of how ROS accumulation relates to DN, our central nervous system findings enable us to speculate towards a mechanism for this pathology. We propose that increased neuronal activity results in increased demand for ATP, increased mitochondrial activity, and ultimately increased oxidative stress. Without SOD2 to detoxify ROS, mitochondrial and cellular damage ensue. This would also apply to actively myelinating Schwann cells, as peripheral myelination is one of the last maturation events in the nervous system, and is supported by our current peripheral nerve findings and a recent report of decreased energy capacity in cortical synaptosomes isolated from SOD2-null mice (Flynn et al., 2011). In conclusion, excess oxidative stress causes mitochondrial disruption and deficits in mitochondrial enzymes and/or decoupling which result in increased oxidative stress. Which event occurs first in the development of neurologic disease is the subject of enormous study and debate.
This work utilized the Morphology & Image Analysis (MIAC) of the Michigan Diabetes Research and Training Center funded by grant DK020572 from the National Institute of Diabetes and Digestive and Kidney Disease. This work was supported by the National Institutes of Health (NIH UO1 DK076160-05) and the Program for Neurology Research and Discovery (www.pfund.umich.edu).
Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
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I am a fine art photographer who lives and works in Geneva, the French west side of Switzerland. Member of the "Société Genevoise de Photographie" since 1958 and of FIAP, I started in the early 60s devoting my time to the probably most difficult task in photography, the representation of the female body. The essence of my relationship to the nude is the photographic exploration of the female body and its architecture to natural specific environments, at dawn or twilight when light is at most interesting. The female body remains my passion as a form of aesthetic research and a way to express emotions and fantasies. My view of the nude is an eternal "homage to womanhood", drawing out the best of each model. Technically self-taught, I always works alone with amateur models who enjoy being photographed. My work in B&W and colour has appeared in many magazines, books and national/international competitions and I had organised several solo shows in galleries. More pictures can be seen on my personal website
External Links
- Aphroditephoto - personal website


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Lincoln Center's second tribute to animation's bop-bopping history, "Cartoon Musicals II" (December 28 through January 4), showcases everything from Betty Boop's first foray into color to South Park: Bigger, Longer & Uncut. One of the unparalleled gems is a collection of films from the '30s and '40s by master animator Oskar Fischinger, presented in association with Los Angeles's Center for Visual Music. Fischinger created what he called "absolute animation," inventing techniques to give life to his spectacular visions. Imagine the paintings of Malevich or Kandinsky swirling into psychoactive phantasmagoria, choreographed to symphonic music or jazz, and you'll only begin to understand his achievements. He's not only one of the greatest abstract painters of the 20th century, but also great-granddaddy to the neo-psychedelic boomlet of recent artists like Paper Rad, Jeremy Blake, and Forcefieldnot to mention iTunes Visualizer. In the monochrome Study No. 6, tadpole shapes zip around, transforming into dancing chevrons and lunettes, while Composition in Blue animates clay forms into a three-dimensional proto- Gondry rainbow waltz. Radio Dynamics, presented in a new print, may be Fischinger's masterwork: A silent symphony of pulsating concentric circles and fluttering trapezoids, this multichromatic wonder pushes animation from mere entertainment into the realm of spiritual ecstasy.
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Klemchuk LLP was formed with the goal of building the most innovative intellectual property law firm in Dallas. Please read more to see how we plan to achieve this mission. Read More
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Want Pizza, Burritos and Booze Delivered This Weekend? There's eCommerce for That. - The explosion of eCommerce continues to transform how we connect with everyday products and services. When Amazon started selling books online in 1994, few (except perhaps Steve Jobs) could envision our phones would be smart, and we'd have apps to order pizza or a case of beer, but that's all possible today. If you're launching an eCommerce strategy , you can learn a lot by examining the approaches taken by food and beverage players Dominos, Chipotle and major...Read More


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Read the latest tweets by Phil Hellmuth. Each new tweet will be picked up by this page. Phil Hellmuth tweets under the alias @phil_hellmuth on Twitter. Check the high stakes twitter-feed for more poker twitter.
RT @RealKidPoker: I will be on a California Tour very soon! Details here: http://t.co/KZMcJLK9KZ visiting local card rooms and talking onli…
Online poker is back!! Sort of: I just played $1,000 WSOP NoLimHold'em tourn here in Las Vegas. Can't wait for full legalization in USA!
In @WSOP $111,111 buy in #OneDrop, end of Day 1: 135 started, 46 left, 16 get paid. I have 1,395,000 in chips, ave 900,000. \n#POSITIVITY
At dinner break in @WSOP $111,111 buy in #OneDrop NoLimHold'em Tourn w 350,000ish in chips. About an Ave stack.\n#POSITIVITY #NoExcuses
RT @MayorSlay: Happy to present a Key to the City tonight to Chaminade alum and current Warrior, @Dlee042. #fgs http://t.co/Inc47Ex0N8
RT @Sonya_Siren: LOL at these two ?? @brianchastings @phil_hellmuth #OneDrop http://t.co/B5NYZ5EAy5
I'm at the beautiful @AriaLV pool catching some sun, chilling, resting up, and refueling for today's @WSOP $111,111 buy in #OneDrop
End Day 2 in @WSOP $2,500 NLHold'em Tourn: 41 left. I'm 4th in chips w 622,000 #PlayGreatPoker #WSOP15in2015!!\n#NoExcuses #POSITIVITY
@Joeingram1 @Oscillator_WSOP @GSinishtaj wasn't big timing anyone, just trying to not talk to anyone and stay focused on Bracelet 15!
@Juicemanpoker Tyty #POSITIVITY
@scottlstuart Tyty for the link!! #POSITIVITY
@Cjenks863 @WSOP Tyty #POSITIVITY #NoExcuses
Just hit my 112th @WSOP Cash in $2,500 NLHold'em. 120 left, 9 Final Table. I'm top ten in chips! #NoExcuses #PlayGreatPoker #POSITIVITY
In @WSOP $2,500 NLHold'em, 1244 started, 230 left, 144 get paid. I have 134,000, ave is 66,000. #NoExcuses #POSITIVITY #PlayGreatPoker
Doing everything right...From great play to max sleep. Great mix of fun, days off, naps, and sun. Might just snap off @WSOP Bracelet 15!
RT @ucanthateme: Did I really just hear @AndreIgoudala9 just drop @phil_hellmuth 's name on @JimmyKimmelLive and "white magic" please Phil…
Congrats @BrianCHastings on @WSOP 10 Game Mix Win: his 2nd 2015 Bracelet! Good guy: well liked by all. @RealKidPoker at Final Table now!
@LisaPavin Your husband is doing a great job on the FOX US Open coverage Lisa! #CoreyPavinUSOpenChamp
Watching US Open golf coverage in bed in pajamas w wife, and room service. Next I'll nap and then hop into @WSOP Players Championship
NBA Finals MVP @Andre is still hot! He saved our team - @hbarnes and I - in our golf match at Shadow Creek w clutch shots #GreatTeammate
RT @nickabourisk: @phil_hellmuth rest up, #MANUP, and Warrior up Phil, because there's no stopping you in the Poker Players' Championship!!
Happy Fathers Day Dad! I love you and I have massive respect for you. TY for teaching me, and Ty for all of the love and support!
RT @beingdagreatest: the life of a champion: you guys go through the drive through @McDonalds--I hop on a private jet for an egg mcmuffin! …
27 hrs away from @WSOP after 17th place Friday. Had fun w @Warriors, napped, watched golf & movies. Sunday is $50K Players Championship!
PIC: @Dlee042 @Money23Green and me. Liquid Pool @AriaLV now! #POSITIVITY #NBAChampions #DayOffFromWSOP http://t.co/JdFMs5uO3T
PIC: @Money23Green me and @KlayThompson at Liquid Pool at @AriaLV. #WorldChampions @Warriors! http://t.co/TV6Fva1K2l
PIC: me, @AndrewBogut and @Money23Green at Liquid Pool at @AriaLV. #NBAChampions @Warriors #POSITIVITY http://t.co/uW32xaXZ1m
PIC: walked into Liquid at @AriaLV now w @Warriors Players and was greeted by this: http://t.co/8FYbzkeBwa
PIC: Team World Champion @Andre Me and @hbarnes @Warriors. #NBAChampions Golfing at Shadow Creek 3 v 3 Scramble http://t.co/Ot5nzEpQhu
PIC: WARRIORS up w DJ! Bogey, DLee, Draymond Green, Klay Thompson, Iggy, Livingston and Ezeli http://t.co/5jNpkCxg8q
PIC: bottle service Marquee: girl is wearing @Warriors Jersey. Partying w a bunch of newly minted NBA Champions! http://t.co/jLrHktzOqA
Sometimes great play is not rewarded: that's poker. Sad to be out in 18th place in @WSOP 10 Game Mix. Time to party w the @Warriors
Just cashed in @WSOP 10 Game Mix Tourney, my 111th all-time WSOP cash. 42 left, 6 make Final Table. I have 88K, ave is 68K\n#POSITIVITY
RT @beingdagreatest: Going out with my boys tonight in Vegas baby! http://t.co/vUqTPqWHxh
You forgot Mayweather!! He's in the PIC on the left...RT @beingdagreatest Three champions in one photo!!! http://t.co/EQzMqjxycM
RT @KevinLiao_: LOL! Draymond: "Bogut is better than @phil_hellmuth [at poker]." #WarriorsParade
Giving credit to @WSOP staff for listening to the players and improving the structures so that we have more play LATE in tourneys.
In @WSOP 10 Game, 380 started, 89 left, 42 paid. Ave 32K, I have 34,200. Great NRG from my parents, and my best friends here in Vegas!!
In @WSOP $10K 6 Max NoLimHold'em, 259 started, 102 left, 30 paid. I Did not close well in Hold'em in 2014 WSOP: it's 2015, time to close!
PIC: Mom and Dad: My parents are in town!! Happy 75th birthday to my mom! #AlwaysClassy https://t.co/NBRKm9SZ3z
End of Day 2 of @WSOP $3,000 HORSE Tourn I have 17,200, ave 23K. 376 started, 242 left, 40 get paid. #JustWin #NoExcuses #POSITIVITY
PIC: mad respect for this guy. I'm next to @Warriors Owner Joe Lacob & @westcoastbill, @FloydMayweather on left http://t.co/S9NKfEHwo0
RT @chamath: What a team. What a season. What a memory. #Unbelieveable #DubNation \n\nNOW BRING OVER THAT RING!!!
So proud of @Warriors Owner Joe Lacob (& Nicole), GM Bob Myers & his 2nd in command Kirk Lacob! Proud of coaches & players! #TeamEffort
Busted @WSOP 6 Max NLHold'em Tourn, my A-Q vs 9-9 all in pre. A lot more frustrated than I should be!!! At least I can watch @Warriors
RT @PokerNightTV: Last week, @phil_hellmuth won a bracelet. \nThis week, his team can win a ring.\n#warriors #manbling http://t.co/qEpQsTFGhI
RT @Balsbaugh: The team running the ill fated Trump Poker once told me that Donald Trump really enjoyed watching @phil_hellmuth play poker …
Here we go again? 32 left (772 started) in @WSOP HORSE, Friday going for WSOP Bracelet #15, AND 2 bracelets this week!!!\n#POSITIVITY
RT @SportsCenter: Dub Nation roars back. Warriors take back home court with 103-82 win over Cavs in Game 4. #NBAFinals is tied, 2-2. http:/…


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Champions League - Dortmund grab lifeline
Borussia Dortmund kept alive their hopes of qualifying from Champions League Group F with a vital 1-0 home win over Olmypiacos thanks to Kevin Grosskreutz's superb early strike.
The tie at Signal Iduna Park an important one for both sides as they chase Marseille and Arsenal at the top of the group.
Dortmund should have found themselves in front after just three minutes. Ivan Perisic received the ball 10 yards out but the Croatian midfielder struck his effort over after the ball had been pulled back to him.
Grosskreutz then gave the German champions the lead after just seven minutes. Mario Goetze teed up the young German and he blasted his effort from 25 yards past the helpless Balazs Megyeri in the Olympiacos goal.
Olympiacos had chances of their own as they looked to counter the dominant Dortmund.
Kevin Mirallas almost beat Roman Weidenfeller at his near post from a low strike but the German keeper gathered the ball at the second attempt.
The hosts looked more comfortable on the ball but neither side were creating any clear-cut chances in the first half although an off-balance Francois Modesto went close for Olympiacos when he blazed over from close range.
Into the second half and Olympiacos almost got straight back on level terms. Jose Holebas found himself in enough space to get a shot off but his low fierce strike was pushed away by Weidenfeller.
Down the other end the impressive Moritz Leitner brought Megyeri in action after a quickly-taken free-kick, with the keeper collecting the ball with ease.
On the hour mark Robert Lewandowski almost doubled Dortmund's lead as the striker was aware enough to intercept a poor back pass. The Polish striker rounded the keeper but saw his shot rebound off the post.
Olympiacos began to grown into the game as it went on and went close themselves when Holebas got his head to a ball from a corner but he saw his effort go narrowly wide of the Dortmund goal.
In a game of few chances it was clear that both sides would have to make chances count and deep into stoppage time Olympiacos failed to do so. A long free-kick was pumped into the box and Olof Mellberg got his head to the ball, but the former Aston Villa man could not direct his header on target.
It was a huge win for the German champions: the win not only ensures they are still playing for a spot in the last 16 of the Champions League, but also boosts their chances of qualification for the Europa League with a third place finish.


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Problems with Hydrofracking & More in Today's News
State - One of the biggest problems with shale gas drilling or hydrofracking is how to handle all the contaminated wastewater.
State - One of the biggest problems with shale gas drilling or hydrofracking is how to handle all the contaminated wastewater.
New York - After two years of tense negotiations between state officials and union leaders, an agreement over how New York teachers will be evaluated has been reached.
Do you feel confused in the morning? Do you feel sometimes like you've been shot out of a cannon-unable to think straight? You're not alone. Mornings CAN ruin your creativity. Check this study out (unless you're in a rush- I'll try to make this quick)
National- A man in Washington deliberately set fire to his home, killing himself and his two sons. Josh Powell was the lone suspect in the 2009 disappearance of his wife, Susan Powell.
National- American Airlinesannounced Wednesday it plans to cut 13,000 jobs from their staff. The airline is the third largest in the nation with 88,000 employees.
I had the chance to sit next to someone during my recent appearance at the Cohoes Rotary last week. We got to talking, and I asked what her occupation was. The answer she gave made me want to whip out my laptop and start blogging right then and there!
Not only is a picture worth a thousand words, but sometimes so is a word itself. This will make sense in a second, I promise!
In honor of the upcoming Health and Beauty Expo (information right here), I have decided to try to make a change from my former self- the person always looking for the easy way out when it comes to exercising. These are the exercises that I do now
On Saturday, January 21 GNA presents the Fitness, Health and Beauty Expo at the Empire State Plaza in Albany. If you're looking for some motivation to get back into shape this year, just read this amazing story of a Best Fitness instructor who transformed his body and life!
This is a true story. You can't make these things up. I was more embarrassed than I've been in a very long time, but it convinces me even more that some things happen for a reason. Out of all places, it happened on the campus of St. Jude's Children's Hospital.
I had the unique opportunity to visit this amazing facility almost 20 years ago, so I'm interested to see the changes. It's still extremely vivid in my memory. We went room to room and visited patients, the familes of, and committed doctors as well. It should be amazing!
As we all focus on turning many new leafs this new year, here are a couple of quick facts to help on some of the inevitable health and fitness goals we all so desperately desire! Compliments of BEST FITNESS, and for more information on how to get fit and stay fit all year, visit their website.


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You can now invite anyone to meet at your favorite Starbucks.
Limited-time feature
33 year old man | West Covina, CA, USA | Seeking women 21-30 within 75 miles
Work hard, play hard!
I'm a pretty easy going guy that likes to laugh a lot and make people laugh around me, but can be serious when i need to be. I also enjoy spending time with my family with whom i'm pretty close too, but once u get to know them you'll likely be close to them as well ( their very inviting hehe). I'm looking for a girl that will make me laugh and have a good time with (possibly with a little nerd mixed in, but not a deal breaker). I'm also a bit of a gear-head at heart, but life is too busy atm to get back into that. I have goals in life that i will achieve (if your wondering just ask) that i'm currently working very hard at.....really i'm just an old-romantic at heart ;)
Camping , Coffee and conversation , Cooking , Dining out , Movies/Videos , Museums and art , Music and concerts , Exploring new areas , Nightclubs/Dancing , Performing arts , Playing cards , Shopping/Antiques , Travel/Sightseeing , Video games , Watching sports , Wine tasting
Auto racing / Motorcross, Baseball, Basketball, Billiards / Pool, Bowling, Cycling, Football, Martial arts, Other types of exercise, Running, Skiing, Soccer, Swimming, Tennis / Racquet sports, VolleyBall, Walking / Hiking, Weights / Machines
I exercise 5 or more times per week.
Don't display my sign
I like Birds, Cats, Dogs, Exotic Pets, Fish, Horses, Other
Middle of the Road
Vegas, Napa valley, Italy, Ixtapa, beach, Pasadena, the block, Fullerton, Santa Monica
Most forms of music, (action, suspense,romance) movies, sushi, italian , french (most foods lol)
Movies, reading, wine tasting, cooking, travelling, hiking, camping, being a nerd, cars(a bit of a gear-head), working out, sports, art
Decipher by Stel Pavlou
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|6'0" (182cms)
|5'0" (152cms) to 5'9" (175cms)
|Body type:
|Athletic and toned
|Slender, About average, Curvy, Athletic and toned
|No preference
|Light brown
|Auburn / Red, Black, Light brown, Dark brown, Blonde, Salt and pepper, Silver, Dark blonde, Grey, Platinum
|No Way
|No preference
|Social Drinker
|Social Drinker, Regularly, Moderately
|Executive / Management
|No preference
|I'll tell you later
|No preference
|Never Married
|No preference
|Have kids:
|Wants kids:
|White / Caucasian
|No preference
|Christian / Catholic
|No preference
|Arabic, English, French, German, Italian, Portuguese, Tagalog
|Some college
|No preference


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The Brookfield East H. S. (BEHS) Forensics team had quite a day yesterday winning the James Madison Memorial (JMM) Forensics tournament with 310 points overall. This is another strong finish for the team that has consistently been posting 300+ point finishes this season. There were 32 Spartans competing in 13 categories in the final rounds. In the category of Radio, the team swept again this season going 1, 2 and 3. The team's 1st place finishers included Lisa Browne, Noah Haselow and Heather Patterson. A highlight was all 3 of the BEHS Group Interpretation teams making it into the finals.
Below is a complete list of all the forensicators who made the final rounds.
1st: Lisa Browne
2nd: Alaina Beau
3rd: Bashar Zaidat
3rd: Ashveer Singh
2nd: Kali Carrasco and George Black
3rd: Mihir Trivedi
Finalist: Will Bartkowski
Finalist: Kali Carrasco
4 Minute:
Finalist: Ryan Wolff
Group Interpretation:
2nd: Katie McKibbin and Katie Seim
Finalist: Noah Haselow, Niyaz Nurbhasha, and Will Bartkowski
Finalist: Eva Juniel, Alaina Beau, Nadia Vogel, Maria Harder
Moments in History:
2nd: Jenna Brockmann
Oral Interpretation of Literature (OIL):
3rd: Kali Carrasco
Finalist: Apoorva Selvaraj
Play Acting:
Finalist: Bella Hyndiuk and Michelle Lazar
2nd: Nihal Nurbhasha
Finalist: Rija Rehan
1st: Noah Haselow
2nd: Jonas Demuri-Siliunas
3rd: Andrew Kluck
Special Occasion:
3rd: Steve Browne
1st: Heather Patterson
Finalist: Kaity Kugler
See all the JMM tournament results here:
Next tournament: Whitefish Bay Invitational on Saturday, March 8th, 2014
Follow the team on facebook to keep up-to-date:
Photo credit: Michael Maier, Maier Learning Media
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- Fourth of July, Festa Italiana, Good Health and Good Deeds … All Together!


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A facial combining two of Morocco's finest treasures.
Ghassoul Clay deep cleanses the skin, repairs damaged tissues and eliminates accumulated toxins whilst Argan Oil moisturises and softens the skin.
Nail Salon
Online bookings let you pick date/time for your visit.
If you purchase an eVoucher you will need to book with the venue directly.
Our old Wahanda Gift Vouchers can still be redeemed at over 3,000 locations. Use them to pay as you would with cash. Please note, the Wahanda Gift Voucher's successor – the Wahanda Gift Card – can only be used for online purchases.


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Updated 06/20/2013
The specialty at one of the Paraty's most elegant restaurants is camarão casadinho, fried colossal shrimp stuffed with hot farofa (cassava flour). Even if you're familiar with jumbo shrimp, you might be astonished at the size of these beauties. Seafood outnumbers other dishes two to one, but the filet mignon is very good. Glass doors that open onto the street are both welcoming and lend a meal here a touch of grandeur, as does the gracious, professional service.
Praça da Matriz, Rua Marechal Deodoro 233, Paraty, 23970–000, Brazil
Website: www.hiltinho.com.br
Restaurant Details:
Updated 06/20/2013
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LoveSpell (Type): We've been inspired to action by customers asking for an 'inspired by' fragrance of Victoria Secret's Love Spell fragrance. We love our version of this popular scent. It has fruity sweet notes of Italian Bergamot, White Lady Peach and Strawberry mixed with middle notes of Apple Blossom, African Tamarind and a touch of Damask Rose. The base is Tonka Bean, Coconut Milk and White Musk. It soaps like a dream in cold process but does discolor eventually to more of a bright yellow color after time.
Photo Attribution: © Can Stock Photo
Terah from Bramble Berry replies...Hi Lyndan! I'm glad you thought our LoveSpell (Type) Fragrance Oil smelled nice out of the bottle! Though, I'm sorry that it did not come across in your soap. We found this fragrance had a strong scent in cold process soap when used at .8 oz per pound of soap found using our Handy Dandy Fragrance Calculator. I will email you personally to help troubleshoot!
Reply from Bramble Berry Hi Kelly! I'm so sorry you don't like this fragrance. I'm also sorry you did not receive the email I sent to you to discuss the LoveSpell (Type) Fragrance Oil further. You should have received that email on 3/17. I have also sent another email today and left a note on your most recent order so you would have the best chance of receiving my message! I look forward to speaking about this with you!
"Thank you for your prompt response. Bramble Berry has the best customer service of any company I know of. Pass the word!" -Rene
Margaret of Sweet Soft Skin Handmade Soaps creates gorgeous soap that is full of color and personality. Her signature style are bars with plenty of layers, soap frosting, embeds and glitter. The result is showstopping! Read on to learn more about Margaret, including what inspires her color palettes and her number one soaping tip! -A.M. […]
The post Interview with Margaret of Sweet Soft Skin Handmade Soaps appeared first on Soap Queen.


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Jesus' resurrection was only the beginning of the story! Join us here at The Shores Church as we begin a brand-new sermon series: A.D. This new series is based on the upcoming television series, A.D. (on NBC beginning Easter Sunday, April 5, and running through June 21) and covers the first ten chapters of the Book of Acts. Produced by Roma Downey and Mark Burnett, A.D. picks up where The Bible mini-series left off, and explores the epic journey of the first followers of Jesus. Millions of people will be watching the TV show and will want to learn more about what the birth of the early Church means for their lives today. Invite your friends, family, and neighbors to come learn more and grow in faith as we study these life-changing stories from Acts.
The Shores Church is excited to host Crocodile Dock Vacation Bible School on Monday, June 15th- Thursday, June 18th 6:30pm- 9pm! Join us on a wild adventure in the bayou where fearless kids learn to shine God's light. Every night kids will learn that God is with us, God is powerful, God does what He says He'll do, God gives us life, and God cares for us. Geared for children ages 4 years through just finished 5th grade. Each night kids will dig into God's word, sing praise and the top of their lungs, munch tasty snacks, engage in team-building activities, and have tons of FUN!
"...if my people, who are called by my name, will humble themselves and pray and seek my face and turn from their wicked ways, then I will hear from heaven, and I will forgive their sin and will heal their land."
Have a Bible Reading Plan Yet? One of our core values is Bible reading. We encourage you to get into God's Word every day. This App makes it extremely easy! If you do not already have a Bible app, or do not have this one, You Version is a great bible application. We highly recommend it! Just click HERE or on the YouVersion logo and we will take you there.


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2 bedroom flat for sale Hadrian Flats, HaltwhistleOpportunity to purchase one of three apartments, converted from a former Mill. Situated to the first floor, with two bedrooms, this property must be viewed to appreciate the location, size and views on offer. The accommodation is light and spacious, benefiting from gas central heating and double... More details ›
3 bedroom apartment for sale Shepherd Terrace, Haltwhistle**UPPER MAISONETTE-POPULAR LOCATION-ALLOTMENT GARDEN**Immaculately presented three bedroom first floor flat situated in Haltwhistle, the property which is over two floors has been updated to a high standard and benefiting from upvc double glazing and gas central heating, briefly comprises of More details ›
2 bedroom apartment for sale Park Road, Haltwhistle, NE49This two bedroom flat is one of six situated in The Mill on Park Road, Haltwhistle with access to local amenities. Available to buy now after being recently fully refurbished, this property benefits from having gas central heating and double glazing. There is a monthly maintenance charge of ?? More details ›
£79,950 Offers Over
Offers Over £79,950
2 bedroom apartment for sale Capel Avenue, Haltwhistle, NE49This two bedroomed upper flat is located in Haltwhistle and offered for sale with NO FORWARD CHAIN. Benefitting from double glazing and gas central heating the accommodation briefly comprises,: Entrance Stairway, Inner Hallway, Lounge/Dining Room, Kitchen, Two Double Bedrooms and Bathroom. Extern More details ›
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Nose: Sweet cure bacon, smoke, malt, sweet peat, touch of caramel.
Palate: Soooo fruity! Peat doesn't even get a look-in. Lots of malt, tart.
Finish: Medium, peat emerges as the end, fruit teas.
Overall: An excellent nose.
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The legal stuff: not providing your contact details may mean we have to delete your
comments if another member of our community complains about them. Whatever happens though, we'll never share
your contact details unless you specifically tell us we can.
Please click here for more details.
very nice. Dam good whisky.
27th February 2015
Had only tried the Fusion earlier and now got it as a gift from my bro in law who lives in Bangalore. Wonder why is it so diifficult to get these superb Indian malts in India. Enjoyed every drop and found it extremely smooth. Way to go Amrut....
25th May 2014


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You can now invite anyone to meet at your favorite Starbucks.
Limited-time feature
38 year old man | Winston-Salem, NC, USA | Seeking women 26-40 within 60 miles
Active within 24 hours
Soooo you're saying there's a chance?
I would say my most prevalent characteristic is my sense of humor. I'm a complete jokester and I just try to enjoy life no matter the circumstance. Just trying to find someone who is looking for the same things as me. I'm very active and enjoy playing sports,traveling, and enjoying the outdoors...would like someone who shares these interests. I tend to gravitate towards women who are different/unique. I'm not into the super shy type or people who have a narrow point of view of the world. I'm single by choice - I'd rather be single and look for something great rather than settle for something decent. Sucks having standards! Ha ha I have a great career but I don't take myself too seriously. I just enjoy having fun and living life!
Camping , Coffee and conversation , Cooking , Dining out , Gardening/Landscaping , Movies/Videos , Museums and art , Music and concerts , Exploring new areas , Nightclubs/Dancing , Playing sports , Shopping/Antiques , Travel/Sightseeing , Watching sports , Wine tasting
Baseball, Basketball, Football, Golf, Hockey, Martial arts, Other types of exercise, Running, Skiing, Walking / Hiking, Weights / Machines, Yoga
I exercise 5 or more times per week.
I don't believe in astrology
I have Dogs
My doggers is my world! She's my little buddy and she thinks we're dating or something. Kinda weird.
Middle of the Road
I love traveling....I have visited Japan many times (Osaka, Kyota, Nara are the best places to visit), Thailand (Kyo Phangan is tops), Puerto Rico, Bahamas, Mexico, europe,and all over the U.S. Love to travel.
I like all types of music but I mostly listen to hip hop and rock. T.V shows...hmmm... All sorts but I love Family Guy, South Park, Dexter, Seinfeld, Entourage, etc.. I do like to cook sometimes and I can make some mean dishes!
I enjoy all types of sporting activities like snowboarding, golf, basketball, football, etc... I like the outdoors so the beach and hiking in the mountains r fun. I travel a lot too. I'd love to find someone open to trying new things together!
I like to read historical based books or suspense type fictional books
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|5'10" (177cms)
|5'0" (152cms) to 5'11" (180cms)
|Body type:
|Athletic and toned
|Slender, About average, Athletic and toned
|No preference
|Dark brown
|No preference
|No Way
|No Way, Occasionally, Yes, but trying to quit
|Social Drinker
|No preference
|Technical / Science / Computers / Engineering
|No preference
Spent about 10 years doing automotive engineering (design), now I've moved down to N.C. to try something new with aerospace engineering
|$75,001 to $100,000
|No preference
|Never Married
|Never Married, Divorced
|Have kids:
|Yes, and they sometimes live at home, No
|Wants kids:
|I'll tell you later
|White / Caucasian
|No preference
Funky white boy
|No preference
My "religion" is karma based
|English, Japanese
|Bachelors degree
|No preference


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A family tradition of World Class hearing care
Parker Hearing & Speech has been servicing the hearing community of South Bay for 35 years providing a full range of hearing care services Our experienced clinical professionals provides complete hearing care services including prevention, diagnostic care, hearing aids, counseling and personalized aftercare to patients of all ages. Our specialized department offers the most advanced technology in equipment and testing to give you the best hearing solution options that are available. We can help you make the best choices with all of your hearing needs.
We have 3 locations to better serve all of your hearing needs.
»Call (310) 803-9908
(4.9 miles)
We are the South Bay's favorite hearing aid providers. We were voted the Best Audiologist of the South Bay every year since 2009. ...
»Call (310) 803-9895
(5.9 miles)
William Lee Parker, PhD and his staff at Parker Hearing Institute are highly skilled at diagnosing and treating all types of hearing loss. From ...
»Call (562) 472-0460
(8.1 miles)
Serving the Long Beach and Los Alamitos area for the past 15 years.


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First pictures of Chris Evan and Chris Hemsworth fighting together on the 'Avengers' set. The pair were in their respective costumes as superheroes Captain America and Thor as they battled alien soliders in the streets of a mock 42nd Street in New York City. They had a lot of fun on the set practicing their moves with their stunt doubles also and villians.
Yep, the whole world just saw Tyga's penis.
Chrissy Teigen just added to the thirst that is currently erupting on Instagram.
Celebs have really hot siblings, and we think you should know about them!


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Frederick Olson, Automotive Contributor
A man's journey into the history books is about to take a pit stop in Hawaii. The solar powered plane that's trying to be the first to ever fly around the world is here in the islands.
This weekend, more than 200,000 aging hippies, jam-band fanatics and got-to-be-there scenesters will pack Chicago's Soldier Field for three farewell concerts by the Grateful Dead. For many of them, it won't be cheap.
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Spread the words to all your frens at campus and fothang, esp those who are veggies to join lah yao! okay? This is a community esp for veggies. And... Let's go toward vegan, step by step, for a healthier living
Ask me
None reviewed yet.
None yet. Ask me to upload some.
Please ask me to submit some.
Reasons why I prefer to eat vegetarian or vegan:
My own faith..
I joined HappyCow Members Community because:
I wanna share about my own experiences..
Here are some places I've been that I liked... and places that I would like to visit one day:
no answer yet
Some of my favorite veg*n foods include:
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Here are some books, movies, magazines, and my favorites that I would recommend to other HappyCow members:
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From topaz_
Thanks for your love and support on my set! <3
Thanks for your love and support on my set! <3
The goal of this set is not FP it's spreading love all around us <3 If it ever reach FP, then it's a little extra!
Thank for your love <3
Thanks for following ❤ ❤
Hey babe !! I hopefuls suicide !!! I'm looking for hearts hehe :) you give me one? kisses for you muack
Thanks so much for the love on my new set, you're awesome <3
Has the hood came yet?
Thank you for the love on my set! ❤
Thanks for the follow! :)
Hi there! I just wanna thank you for your love on my first set. Your support mean a lot for me. XOXO.
Thank you so much for the love on my latest set, you're too awesome xxx
Thanks for your love in my set 💕 hope You'll enjoy my next one (a multiset I made with my hopeful friend Lincy ) that goes up in 2 months!!! Have a nice day 💋
Thanks for the follow!
Maybe you trashed it in a good way? Maybe its all growly now? :D
Thanks for the love on my set, hope youre having a good day♥.
Thank you ;)
Hi sweetie I invite you to see my profile 😊 you are very welcome! My debut set is on MR today ❤ hope you like Kisses&Love 😘💜😘💜😘💜
:D :D Thank you so much @catdad for your super sweet comment on my debut hopeful set :) I really appreciate it!! ♥
Thanks so much for your support xx
It's great to see you and thank you for showing me some love.
Thank you for your support, your comment on my set makes me really happy<3
Thanks for the comment on my set :) Always smiling haha
Thanks, I hope I can quit too. It certainly is disgusting!
Thanks for the follow! xo
hello .. if you liked my set " blue" I want you to know that my new set " them nymphes des forets " is now available , I hope you like it thank you very much for the love and support. <3 <3 <3


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Welcome to Providence Hall in Fredericksburg, TX, a safe environment where students are appreciated for their unique gifts and accepted for where they are in their educational journey. Providence Hall is a new school, created to reach not only traditional learners, but non- traditional learners. We daily involve the whole child, acknowledging that the emotional, physical, and cognitive abilities of each student are equally important and should be recognized throughout the teaching process.
The faculty teaches through a multisensory curriculum and encourages students to think beyond the textbook through hands-on activities and engaged learning. Our small class size provide differentiation, individualized instruction, peer teaching, educational centers, and multiple field trips. Providence Hall is founded on Christian principals but welcomes students of any faith or religious backgrounds. We also offer additional services including occupational therapy, speech/language therapy, study skills, and social skills training.
I believe children are a gift from God, and their minds are open to learning when given the appropriate tools and support. It's amazing to watch a child soak up information in new and different ways when given the chance. I invite you to tour our campus and witness the exciting learning taking place.
Lynn Nolen
Head of School
Latest News
Help the School by Shopping AmazonAmazon will donate 0.5% of the price of eligible purchases...Read more
Box Tops for EducationSave box tops to help fund school programs. Click here...Read more
Financial Aid for School TuitionThis year we will utilize the Financial Aid for School...Read more


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123CE.com offers a variety of continuing education courses that are approved by The American Culinary Federation (ACF), the Association of Nutrition & Foodservice Professionals (ANFP), and the National Association of Legal Assistants (NALA).
Our goal is to provide you with a simple way to gain or maintain your certifications. All of our courses are offered in an easy-to-use online format, giving you the ability to choose when and where to complete your studies. Course subjects include the latest in food safety, nutrition, foodservice, business training, paralegal ethics, and more.
123CE.com, formerly known as American Academy of Independent Studies (AAIS), has been in operation since 1984, providing you with the CE credits you need.
ACF certification and recertification courses available. To learn more click here.
ANFP approved courses available. To learn more click here.
NALA approved courses available. To learn more click here.


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- The Big Picture: What's AAP
- A year later, the tweak: Desh to Dilli
- Bus from Burari laden with volunteers and hope
- Rare day out for AAP families
- Riot of support for AAP in communal hot spots
- Hunt on for CM house, will not accept Z-plus security
- No word from high command, Delhi Congress in a paralysis
- Latest News
- Second time at Ramlila Maidan: Hope overrides their doubts
- Kejriwal has no portfolio, will keep an eye on others
- In sea of white caps, BJP troika plans to be 'forceful opposition'
- MP, MLA see Punjab as the next AAP stop
- A year later, the tweak: Desh to Dilli
- Arvind Kejriwal repeats his advice to sting the corrupt, asks police to act against 'goondagardi'
- Proud that one of our volunteers has become Delhi CM: Anna Hazare
- Arvind Kejriwal not to keep any portfolio
- Now an Aam Aadmi Party Cola by beverage-maker inspired by Arvind Kejriwal's party
- New chief minister Arvind Kejriwal holds meetings at Delhi Secretariat
- Cong's Ajay Maken blames Sheila Dikshit for Delhi polls debacle
- Left, right, AAP
The family strikes back
It's been very much a Priyanka Gandhi week.
Let's talk about the week that was. Well, first, it wasn't about Arvind "Cough" Kejriwal. Second, it wasn't about Rahul "Earnest" Gandhi. And it wasn't much about the general elections either. It was more about Robert "Damaad Shri" Vadra, "Honeymoon" Ramdev, Priyanka "Last Congressman or woman Standing" Gandhi and, of course, Narendra "Barrel-chested" Modi. Derek "Quizmaster" O'Brien (TMC) gate-crashed this high-profile headline party by trying to change Modi's professional qualifications from tea vendor to butcher, but he was only briefly breaking news.
And for those of you who are avid YouTube watchers, it might just have been about Last Week Tonight, a new talk show in the US on HBO, with stand-up and now sitting down comic, John Oliver. He devoted over five minutes of the premiere episode to the indifference of the American media to the Indian polls — "the biggest election in human history", "handsome" Rahul, "hologram" Modi and the Foxification of our TV news channels. The latter he illustrated with clips from Times "Shouting" Now matches and CNN-IBN's incorrect graphic, "A Billion Votes", whereas there are 815 million voters. Well, it was fun while it lasted, although it was a bit far-fetched to claim that Modi was fighting this election on the strength of "toilets" for everyone, even as bathroom humour.
From what we watch on TV news, the election campaign is no laughing matter. We saw Ramdev hurt Rahul's feelings by saying he wants to marry a foreigner but his mother wants him to be prime minister first (boo-hoo!), and the feelings of Dalits by saying that Rahul visits their homes for "picnics" and "honeymoon" (for which he'd have to be married first, right?). The BJP insulted the first family's son-in-law to target Priyanka for deriding Modi's 56-inch chest, with its video broadcast on all news channels entitled, "Damaad Shri". That was difficult to engage with for the lay viewer but obviously hit its mark, because Priyanka immediately fired back with her description of the BJP as "panic-stricken rats". It's been very much a Priyanka Gandhi week, with the media — and the BJP — hanging on to every word she had to utter.
That's the thing about this election: leading politicians are using the media to address each other rather than to address the problems facing the people. They are talking to each other and they're talking to the entire nation via TV (or, in Modi's case, holograms). So Priyanka may be delivering an election speech in Rae Bareli, but her remarks are for the BJP and a national audience.
Also, the media is largely indifferent to coverage of the problems facing the people and how politicians on the campaign …continued »


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The Town of Fayette is in the center of the Central Finger Lakes. It stretches between Seneca and Cayuga Lakes, and is bordered at the north by the Seneca-Cayuga Canal.
The Town of Fayette is on the western border of the county and is southeast of Geneva, New York. Seneca was part of the Central New York Military Tract and was first settled around 1790. The town was established from part of the Town of Romulus in 1800 as the "Town of Washington" but adopted the current name in 1803.
The Town of Fayette Highway Department is looking for a Part - Time Laborer for the months of July and August. For more information contact Highway Superintendent William "Bill" Trout at 315-585-6330 or at 315-730-3438.
The Town of Fayette is looking for a Town Historian. The position required doing research and writing skills. The canidate must have the ability to teach, do public presentations, promote tourism and preserve history. For more information contact Town Councilwoman Coreen Lowry at 315-719-2430.
The Town of Fayette Town Board Meeting is scheduled Thursday,July 9, 2015 at 7:00 p.m. at 1439 Yellow Tavern Road, Waterloo, New York 13165. Any interested parties or those with business for the Town Board are welcome to attend and be heard contact Debra Vanni - Town Clerk. For the agenda go to the Town Board Page.
The Town of Fayette Zoning Board of Appeals will meet on Monday, July 13, 2015 at 6:30PM at the Town of Fayette Office Building, 1439 Yellow Tavern Road, Waterloo, NY 13165 where they will have a hearing for Salvatore Gugliemino 3744 Kime Beach Rd, Geneva, NY 14456, Town of Fayette, (Tax Map 18-1-23 ) regarding an area variance.
The Town of Fayette Assessor's office will be starting a data collection project to collect and verify all inventory and data for the town of Fayette. This project will begin this summer and run through the fall of 2015. There will be NO assessment changes due to this project at this time. THis project is to begin the process of starting a revaluation in the Town o Fayette at a later date. If you have any questions please contact your assessor, James Cleere at 315-539-0206.
The Town of Fayette Town Board will have a 2016 budget workshop on Saturday,July 18, 2015 at 8am to 10 AM.
The Waterloo Rotary has donated a Free Library for all in our community to enjoy. It is located at the Fayette Town Office
building on 1439 Yellow Tavern Road. The concept behind the library is for all to have access to books. There are books forall age levels. The library is a give and take concept. If you want a book to read stop buy and pick one up. If you have a book you no longer want but think others may enjoy place it in the Free Library. If you really enjoy the book you are welcome to keep it or return it when you are done. We hope that all will enjoy and use the library.


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Biodegradable Pet Bag Refills
Roll over image to zoom in
Stock up on an indispensable pet care product -- and make "taking care of business" a whole lot easier! Our degradable pet waste bags are lightly scented (floral) and come in a set of seven rolls (15 bags per cored roll). Fits any standard pet bag dispenser.
- 6" X 14" (15.2 X 35.6 cm)
- Imported
For bulk pricing on these and other items, please contact our Customer Service department at firstname.lastname@example.com.
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Recent reviews:
November 17, 2014 - Extremely happy with this purchase. I feel better cleaning up after my pet knowing this bag is biodegradable and has a great scent too!
June 9, 2014 - they are strong, smell good....and are big so no worries...love them...
March 2, 2014 - I bought the red bags and they fit perfectly into my dispenser. I like that they are easy to remove at the perforation without tearing into the baggie. I've had that happen with other baggies and it's just a waste. They smell nice and are the right thickness too.
January 3, 2014 - great to carry with you when walking your pet - fits well into pet bag dispenser - scent is OK
January 1, 2014 - These bags are great. I like how strong they are, and that they are biodegradable. The only thing that puts them at a 4 star rating is the scent. Yuck. Unscented, please!
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need to check my call log asap, please help me step by step.
Try these steps:
Sign into sprint.com
Click on to the see my bill link under the monthly fee it will bring up your monthly statement
Scroll done to the line that says call details click the link select number if offered
New screen comes up with the call detail for last month.
Check out a fun infographic that teaches you how to increase storage, enhance security,
improve battery life and clean your device.


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YMMV: Silent Ponyville Chronicles
- Arc Fatigue: Reunion is a massive Door Stopper that started over a year ago and is still going.
- Broken Base: The Ending of Silent Ponyville 3 was not received well, and even the writer himself said he had bit off more than he could chew and wasn't really happy with it either, and wasn't sure if he even could've ended in a way he was happy with due to how things played out. Mainly caused by Spike being Killed Off for Real and Twilight growing to hate Celestia after finding out she's a Retired Monster
- Complete Monster: The serial killer the Colt who targets foals and butchers them alive, eating them afterwards. He even feeds a unknowing Pinkie Pie a Cupcake made from her own little sister, whom he killed right in front of her.
- Harsher in Hindsight/Hilarious in Hindsight: The story starts off with the protagonist conspiring with prison guards to get "alone time" with a convicted child murderer, whom he brutally murders, setting the later events in motion. One year later, cue Silent Hill: Downpour...
- Hell Is That Noise: The air raid siren announcing an impending world shift.
- Whenever Lance listens to his memories of his wife, half-locked behind doors with the number 303 in the apartment complex, a loud ringing in his ear cuts the voices off and catapults him out of the memory. It's likely his subconsciousness's attempts to keep him from remembering.
- Jerkass Woobie: Lance Strongshy of Silent Ponyville 2 has traces of this, but this is brought out more in Reunion.
- Also, Demitri from Blood Chronicles. Whether he's more of a Jerkass or a Woobie depends on which ending you get.
- Magnum Opus: This is considered Sam Rose's best work, in comparison to his other work.


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National Park Authority Members' allowances and expenses published
Tourism attractions raise the bar with Pembrokeshire Pass
Carew Castle and Castell Henllys shortlisted for award
National Park Authority Chairman re-elected
Meet illustrator behind best-selling colouring book at Oriel y Parc
Pembrokeshire has a rich and diverse culture which has been shaped down the centuries by waves of invaders and settlers.
Before the Norman Conquest, the whole area was Welsh speaking and ruled by Celtic chieftains. Entertainment was provided by bards who wandered from court to court recounting folk tales. These legends were later collected as "The Mabinogion".
During the "Age of the Saints" the county was home to a number of important Christian figures, most notably St David, who became the patron saint of Wales. After his death, his shrine attracted pilgrims from far and wide and eventually became the site of St David's Cathedral.
The Norman Conquest created a divide of the county which still remains today. The native Welsh speakers were driven into the wilder areas of the north, while the south was repopulated with settlers from Flanders and Wessex. South Pembrokeshire became known as "Little England", while the more rural north of the county became a stronghold of the Welsh language and culture.


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Hotel Meninas
- Free WiFi
- Airport within 5 mi/8 km: Madrid (XOC-Atocha Train Station)
Main amenities
- 37 guestrooms
- Restaurant and bar/lounge
- Breakfast available
- Sauna
- 24-hour business center
- Airport drop-off service
- Childcare
- 24-hour front desk
- Air conditioning
- Daily housekeeping
- Car rentals on site
- Library
What's around
- In Gran Via
- Plaza Mayor (0.3 mi / 0.5 km)
- Plaza de Espana (0.4 mi / 0.6 km)
- Almudena Cathedral (0.4 mi / 0.6 km)
- Puerta del Sol (0.4 mi / 0.7 km)
- Prado Museum (1.1 mi / 1.8 km)
The hotel is perfectly positioned for sightseeing on foot.the staff were all…
Second stay. Excellent location and well appointed. We had two rooms for one night and…
Enter dates to check prices
Hotel Info: 800 1205131from S$106
- Junior Suite, 2 Single Beds
- Twin Room, 2 Single Beds
- Small Room
- Single Room
- Double Room (with extra bed)
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At a glance
Key facts
Hotel size
- This hotel has 37 rooms
- This hotel is arranged over 5 floors
- Check-in time Noon-5:30 AM
- Check-out time is Noon
- Express check-in/out
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- Babysitting/childcare *
- Pets not allowed (service dogs only)
- Free WiFi and wired Internet access in public areas
- Free WiFi in rooms
- Airport drop off (available on request) *
- No parking
- Area shuttle *
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In the hotel
- Buffet breakfast (surcharge)
- Restaurant
- Bar/lounge
- Coffee shop/café
- Room service (during limited hours)
- Sauna
- Bicycle rentals nearby
- Golf lessons available nearby
- Golfing nearby
- Scooter/moped rentals nearby
- Snowmobiling nearby
- Snowshoeing nearby
- 24-hour business center
- Number of meeting rooms - 2
- Meeting rooms 2
- Conference space size (feet) - 312
- Conference space size (meters) - 29
- Computer station
- 24-hour front desk
- Concierge services
- Tours/ticket assistance
- Limo or Town Car service available
- Dry cleaning/laundry service
- Laundry facilities
- Free newspapers in lobby
- Luggage storage
- Wedding services
- Number of buildings/towers - 1
- Year Built 2005
- Elevator/lift
- Safe-deposit box at front desk
- Library
- Television in lobby
- Accessible bathroom
- In-room accessibility
- Roll-in shower
In the room
- Climate control
- Air conditioning
- Minibar
- Bathrobes
- Slippers
- Iron/ironing board
- Hypo-allergenic bedding available
- Blackout drapes/curtains
- Soundproofed rooms
- Premium bedding
- Private bathroom
- Bathtub or shower
- Free toiletries
- Hair dryer
- 40-inch flat-screen TV
- Satellite TV channels
- Desk
- Free WiFi
- Daily housekeeping
- In-room safe (laptop compatible)
- Access via exterior corridors
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Special features
DiningRestaurante Meninas - This buffet restaurant serves breakfast only.
Cafe de la Opera - This café serves lunch and dinner.
Hotel Meninas's small print
Cash transactions at this property cannot exceed EUR 2500, due to national regulations. For further details, please contact the property using information in the booking confirmation.
For transfers, guests must contact the property before arrival at the number on the booking confirmation.
The name on the credit card used at check-in to pay for incidentals must be the primary name on the guestroom reservation.
Optional extras
Rollaway beds are available for an additional charge
Buffet breakfast is offered for an extra charge of EUR 12 per person (approximately)
Airport shuttle service is offered for an extra charge of EUR 13 per person (one-way)
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.