Mitochondria are cellular organelles that play a key role in several physiological processes, including cell proliferation, differentiation, cell death and the regulation of cellular calcium (Ca2+) homeostasis.
Increases in mitochondrial Ca2+ activate several dehydrogenases and carriers, inducing enhance in the respiratory rate, H+ extrusion, and ATP production necessary for the correct energy state of the cell.
The mitochondrial Ca2+ uptake and release mechanisms are based on the utilization of gated channels for Ca2+ uptake and exchangers for release that are dependent upon the negative mitochondrial membrane potential, which represents the driving force for Ca2+ accumulation in the mitochondrial matrix.
In this thesis, the attention was focused on two mechanisms in particular, the mitochondrial Ca2+ influx system by the activity of Mitochondrial Calcium Uniporter (MCU) complex, and the high-conductance channel mitochondrial Permeability Transition Pore (mPTP), responsible for a state of non-selective permeability of the inner mitochondrial membrane (IMM); its opening in non-physiological conditions leads to Ca2+ release from mitochondria and triggers cell death mechanisms. Thus the maintenance of the mitochondrial Ca2+ homeostasis is essential for a proper balance between cell life or death.
In particular it will be discussed the possible involvement of MCU in the cell cycle, as the Ca2+ accumulation by MCU is important for the regulation of cell life and energy production. It will be shown that MCU is mainly expressed in specific phases of the cell cycle and this expression positive correlates with the mitochondrial membrane potential. MCU overexpression instead does not alter cell cycle phases.
It will also described the role of the c subunit of Fo ATP synthase in mitochondrial permeability transition (MPT) and it will be demonstrated to be a critical component of the mPTP complex. Finally it will be discussed the role of mPTP in mitochondrial Ca2+ efflux and it will be shown that it is a dispensable element for mitochondrial Ca2+ efflux in non-pathological conditions.


A 28 year old female presented with a right renal mass. She was treated with a radical nephrectomy. Histologically the tumour revealed the classical appearance of a carcinoid. The opposite kidney contained a multilocular cystic renal cell carcinoma. The histogenesis of a renal carcinoid is discussed.


Automatic multiple-choice question generation (MCQG) is a useful yet challenging task in Natural Language Processing (NLP). It is the task of automatic generation of correct and relevant questions from textual data. Despite its usefulness, manually creating sizeable, meaningful and relevant questions is a time-consuming and challenging task for teachers. In this paper, we present an NLP-based system for automatic MCQG for Computer-Based Testing Examination (CBTE).We used NLP technique to extract keywords that are important words in a given lesson material. To validate that the system is not perverse, five lesson materials were used to check the effectiveness and efficiency of the system. The manually extracted keywords by the teacher were compared to the auto-generated keywords and the result shows that the system was capable of extracting keywords from lesson materials in setting examinable questions. This outcome is presented in a user-friendly interface for easy accessibility.


Puritans did not find a life free from tyranny in the New World--they created it there. Massachusetts emerged a republic as they hammered out a vision of popular participation and limited government in church and state, spurred by Plymouth Pilgrims. Godly Republicanism underscores how pathbreaking yet rooted in puritanism's history the project was. Michael Winship takes us first to England, where he uncovers the roots of the puritans' republican ideals in the aspirations and struggles of Elizabethan Presbyterians. Faced with the twin tyrannies of Catholicism and the crown, Presbyterians turned to the ancient New Testament churches for guidance. What they discovered there--whether it existed or not--was a republican structure that suggested better models for governing than monarchy. The puritans took their ideals to Massachusetts, but they did not forge their godly republic alone. In this book, for the first time, the separatists' contentious, creative interaction with the puritans is given its due. Winship looks at the emergence of separatism and puritanism from shared origins in Elizabethan England, considers their split, and narrates the story of their reunion in Massachusetts. Out of the encounter between the separatist Plymouth Pilgrims and the puritans of Massachusetts Bay arose Massachusetts Congregationalism.


In this work we intend to show the reflections of the contemporary philosopher Robert Spaemann in what refers to ecology. For this reason, in first place, I will make use of the fundamental ethical category of his thought (benevolent love), as extensible also to the natural world. Following that, I will propose a universal hierarchy of the benevolent love in the ordo amoris. Once we have talked about how to extend this rational love to all beings, I will present Spaemann's view of the world based on two interests of the reason: one dominative and the other one contemplative. To end, I will explain the concept of Mitsein as a proposal for our interaction with the surrounding world.