This review surveys recent publications on the Electrospray Ionisation - Mass Spectrometry (ESI-MS) of drugs of small molecular mass taken from selected drug classes using the Web of Knowledge database. The structural classes are antibiotics/antibacterials, steroids, cannabinols, antidiabetics, immunosuppressants, antitumour drugs, antiretroviral drugs, nonsteroidal antiinflammatories, mucolytic drugs, anticoagulants, cyclooxygenase inhibitors, stimulants, noradrenergic agents, anti-TB drugs and erectile dysfunction agents. Fragmentation information that these drugs exhibit in-source and in ion-trap, triple quadrupole and time-of flight mass spectrometers is also provided. An appraisal of applications for the period 2004-2005, again taken from the Web of Knowledge database, of the technique liquid chromatography - electrospray ionization - mass spectrometry (LC-ESI-MS)to the detection and determination of these drugs, primarily in biomatrices, is then made. Information on such aspects as sample concentration, LC separation conditions, recoveries from biological media and limits of detection (LODs) are provided.


Background: Physical activity (PA) during and after cancer treatment can help with symptom management and reduce the risk of cancer recurrence. However, it is unclear what constitutes an optimal exercise program. In addition, provider and patient barriers exist to the recommendation and adoption of exercise as part of a cancer treatment plan. The goal of this study was to determine how providers and patients feel about exercise during cancer treatment and explore what the barriers to implementing such a program might be. Patients and Methods: Focus groups and interviews were held with patients with malignancy, both metastatic and nonmetastatic, and oncology providers. In total, 20 patients participated in either a focus group or an individual interview and 9 providers contributed to the focus group. An equal number of patients (n=10) were interviewed as attended a focus group. Audiotaped sessions were transcribed verbatim. Theme identification was independently coded by 4 coders and synthesized as a group. Results: Neither patient group recalled PA instruction from oncology providers during their cancer treatment. Most participants (95%) felt exercise is important during cancer treatment, citing overall well-being benefits versus improved disease outcome. Most patients (80%) preferred a home-based exercise program provided by the oncologist. Fatigue was the most cited barrier to regular exercise during treatment (50%). All providers acknowledged benefits of PA to patients, but not universally for all. More than half of providers (55%) preferred a referral system for exercise programs. Clinic visit time constraints and a perceived lack of expertise in the area of PA were common barriers to making exercise recommendations a routine part of the treatment plan. Conclusions: Patients with cancer and oncologists recognize the benefits of PA during treatment. Disagreement exists between to whom, how, and where exercise plans should be disseminated and implemented.


Arthropod-borne disease outbreaks as a result of pathogen influx including arboviruses transmitted by strains of Aedes species occur periodically in varying spots in Kenya. However, there has been paucity of documented information on the Aedes species involved in transmission of different haplotypes of arboviruses. This study assessed for concomitant infection of arboviruses in different vectors. Aedes species were sampled by Bio gent sentinel trap. Mosquitoes were stunned at 4°C for 5 minutes; sorted according to site, species and sex. RNA was extracted using Trizole®. cDNA was generated using one step real time PCR. Proportions of vectors and arboviruses were analyzed by R-statistics. A total of 37, 220 mosquitoes of Aedes species were analyzed. Vectors were: Aedes aegypti formosus (23,265=62.5%), Aedes aegypti aegypti (4,931=13.2%), Aedes mcintoshi (3,557=9.56%), Aedes ochraceus (2,156=5.79%), Aedes pembaensis (2,049=5.51%), Aedes tricholabis (487=1.31%), Aedes albicosta (415=1.11%), Aedes fulgens (200=0.54%) and Aedes fryeri (160=0.43%). Dengue virus (DENV) had highest entomological infections at 79% (n=1910) while Chikungunya (CHIKV) at 21%. DENV-2 had the highest frequency at 54%, while DENV-4 with the least frequency at 6%. Only ECSA genotype of CHIKV was present. Concomitant infections of arboviral haplotypes in different clades of Aedes species occur along the coastal region. Aedes aegypti aegypti is incubates all serotypes of DENV and genotype of CHIK viruses thus the major vectors of arboviruses. This information is important as it gives knowledge on areas at high risk for arboviral disease outbreaks. Consecutively, up scaled survey and implementation of control and prevention measures taken appropriately.


Research on the effects of deicing salts and their trace minerals on bituminous highway wearing courses was prompted by early pavement deterioration of federal and state highways in Indiana. Several bituminous surface courses containing slag, slag-dolomite, or dolomite aggregates showed deterioration soon after deicing salts were first applied. Initially, a detailed literature review (Appendix A of report) was conducted. Data on eighteen dolomite sources from a previous study (Bruner, Choi and West, 1995) plus ten additional dolomite sources formed the basis for the brine freeze thaw testing program. Mercury intrusion porosimetry testing conducted on multiple specimens showed that rock texture significantly affects pore size distribution. Petrographic analysis, acid etching of thin sections and electron microprobe studies revealed the distribution of clays on dolomite grains. Additional brine freeze thaw testing on selected dolomite sources, plus one slag sample, were conducted using deicing salts from six Indiana highway districts. Petrographic evaluation of pavement cores from the distressed pavements were accomplished. Brine freeze thaw test results ranged from 0.6 to 27.3% loss for the 28 samples; this test is a good measure of aggregate durability. The Indiana Department of Transportation has recommended a maximum 30% loss for the brine freeze thaw test. Regression analysis showed that acid insoluble residue content and sodium sulfate loss have the highest correlation with brine freeze thaw loss; elemental magnesium content has a negative correlation with it. It is concluded that dolomite aggregates with a low insoluble residue content have lower sulfate soundness losses, lower brine freeze-thaw losses and greater durability. Evaluation of pavement cores indicated no evidence of deterioration caused by aggregate weathering or degradation. Brine freeze thaw testing is a good measure of freeze thaw resistance of aggregates in bituminous surface courses and no evidence was found indicating that early deterioration of the pavements studied was caused by salt attack.


Graph grammars originated in the late 60s, motivated by considerations about pattern recognition and compiler construction. Since then, the list of areas which have interacted with the development of graph grammars has grown quite impressively. Besides the aforementioned areas, it includes software specification and development, VLSI layout schemes, database design, modeling of concurrent systems, massively parallel computer architectures, logic programming, computer animation, developmental biology, music composition, visual languages, and many others.The area of graph grammars and graph transformations generalizes formal language theory based on strings and the theory of term rewriting based on trees. As a matter of fact, within the area of graph grammars, graph transformation is considered as a fundamental computation paradigm where computation includes specification, programming, and implementation. Over the last three decades, graph grammars have developed at a steady pace into a theoretically attractive and important-for-applications research field.Volume 3 of the indispensable Handbook of Graph Grammars and Computing by Graph Transformations presents the research on concurrency, parallelism, and distribution — important paradigms of modern computer science. The topics considered include semantics for concurrent systems, modeling of concurrency, mobile and coordinated systems, algebraic specifications, Petri nets, visual design of distributed systems, and distributed algorithms. The contributions have been written in a tutorial/survey style by the top experts.


Traffic and emergency monitoring systems are essential constituents of Intelligent Transportation System (ITS) technologies, but the lack of traffic monitoring has become a primary weakness in providing prompt emergency services. Demonstrated in numerous military applications, unmanned aerial vehicles (UAVs) have great potentials as a part of ITS infrastructure for providing quick and real-time aerial video images of large surface area to the ground. Despite of obvious advantages of UAVs for traffic monitoring and many other civil applications, it is rare to encounter success stories of UAVs in civil application including transportation. The objective of this paper is to report the outcomes of research supported by the state agency to investigate the feasibility of integrating UAVs into urban highway traffic monitoring as a part of ITS infrastructure. These include current technical and regulatory issues, and possible suggestions for a future UAV system in civil applications.


The computer game industry requires a skilled workforce and this combined with the complexity of modern games, means that production costs are extremely high. One of the most time consuming aspects is the creation of game geometry, the virtual world which the players inhabit. Procedural techniques have been used within computer graphics to create natural textures, simulate special effects and generate complex natural models including trees and waterfalls. It is these procedural techniques that we intend to harness to generate geometry and textures suitable for a game situated in an urban environment. Procedural techniques can provide many benefits for computer graphics applications when the correct algorithm is used. An overview of several commonly used procedural techniques including fractals, L-systems, Perlin noise, tiling systems and cellular basis is provided. The function of each technique and the resulting output they create are discussed to better understand their characteristics, benefits and relevance to the city generation problem. City generation is the creation of an urban area which necessitates the creation of buildings, situated along streets and arranged in appropriate patterns. Some research has already taken place into recreating road network patterns and generating buildings that can vary in function and architectural style. We will study the main body of existing research into procedural city generation and provide an overview of their implementations and a critique of their functionality and results. Finally we present areas in which further research into the generation of cities is required and outline our research goals for city generation.


Nineteenth-century literary criticism has mainly focused on lasting scientific advancements, at the expense of a more comprehensive history, when examining the legacy of science in fiction. Yet there were many sciences that were considered plausible during the nineteenth century which have since been disproven and the ideas relegated to the realms of pseudo-science. This thesis examines novels by Bram Stoker, Marie Corelli, Florence Marryat, and Arthur Machen with attention to the scientific supernatural. Throughout this thesis, the term "scientific supernatural" will be used to reference mid- to late nineteenth-century scientific investigations conducted by various types of scientists into the supernatural and the set of phenomena that were the subject of these investigations, regardless of the twenty-first century status of the topics under investigation. Phenomena such as mesmerism, clairvoyance, and Spiritualism, which seem to be supernatural in their interactions with material aspects of the world or the supernatural realm, were studied by scientists with the understanding that they were engaged in scientific pursuits. "Scientific supernatural" is, therefore, intended to represent the scientific inquiries into the supernatural and only the areas of study that were, for a time at least, accepted as scientific by some scientists and often by society at large, evident in scientific periodicals, books, and personal documents, into the fin de siècle. Many supernatural elements in literature at the end of the nineteenth century are representations of phenomena that were being investigated by contemporary scientists and, as such, are represented within fiction as having a claim to scientific validity. This term represents the status of the various phenomena in the historical moment where the supernatural realm seemed to be the next place for science to explore.  This thesis is separated into an introduction and three chapters that discuss different depictions of the scientific supernatural. The Introduction surveys criticism of the scientific supernatural and of science in connection with late nineteenth-century literature to lay a foundation of the historical context for this science and establish a gap in current criticism of science and the fin de siècle novel. Chapter 1 explores two different representations of Spiritualism and the way the authors use science to support the worldviews taught through their fiction. The novels discussed in Chapter 2 deal with observed effects of the supernatural in the material world and the problem of explaining these occurrences when science had no certain explanation for them. Chapter 3 examines fictional depictions of scientific experimentation that represent the author's hope that scientific evidence of the supernatural will be uncovered. In each case, the authors suggest there is something yet to be discovered which will allow science to explain the supernatural as definitely real and capable of interacting with the material world.  Fictional representations of the scientific supernatural such as those discussed throughout this thesis reveal a wider understanding of science at the fin de siècle than has previously been addressed in literary criticism. As such, this thesis suggests the need for a broader critical understanding of science, and scientific potential, that mirrors that of fin de siècle English conception of science to more fully inform the scientific legacy left in fiction of the time.


The law should not allow revenge porn in the name of the First Amendment, just as it should not allow private citizens to purchase AK-47's in the name of the Second Amendment. Citizens can abuse fundamental rights just as governments can infringe them. At some point, courts have to acknowledge that the First Amendment was not intended to give people a fundamental right to trash an individual's reputation while seeking cover under the self-serving blanket of opinion and taste. It is one thing to stroll into a courthouse with a shirt that says Fuck the Draft, but quite another to calls someone a Nazi War Criminal. Statements like this poison the marketplace of ideas with the vitriol of immodest jackasses and can, in some circumstances, cause reputational harm. For too long, courts and commentators have reacted in knee-jerk fashion to any attempts to regulate or provide remedies for speech that causes severe and lasting injury. At the same time, however, the courts have founds ways, such as through the "secondary effects" doctrine, to uphold statutes that, as a practical matter, restrict speech. The courts need not go through such legal gymnastics to restrict in one breath what should be regulated in the next.Justice Anthony Kennedy has stated that "[t]he remedy for speech that is false is speech that is true. This is the ordinary course in a free society. The response to the unreasoned is the rational; to the uninformed, the enlightened; to the straight-out lie, the simple truth." Truth is not considered a remedy when courts classify a statement as pure opinion, and pure opinions involving private issues and citizens should not receive the First Amendment's blessing. Simply put, where truth cannot provide a remedy, the law should.


While analyzing shape accuracy of ferroalloy precision castings in terms of ceramic moulds physical anisotropy, low-alloy steel castings ("cover") and cast iron ("plate") were included. The basic parameters in addition to the product linear shape accuracy are flatness deviations, especially due to the expanded flat surface which is cast plate. For mentioned castings surface micro-geometry analysis was also carried, favoring surface load capacity tp50 for Rmax = 50%. Surface load capacity tp50 obtained for the cast cover was compared with machined product, and casting plate surface was compared with wear part of the conveyor belt. The results were referred to anisotropy of ceramic moulds physical properties, which was evaluated by studying ceramic moulds samples in computer tomography equipment Metrotom 800.


This article analyses the dynamics between rainfall variability, food insecurity and human mobility in eight case studies, namely Ghana, Tanzania, Guatemala, Peru, Bangladesh, India, Thailand and Vietnam. It covers a large spectrum of rainfall-related climatic events, including floods, drought, seasonal shifts and dry spells, and their impact on food insecurity and in turn on human mobility in approximately 1300 households in the eight case studies. It also summarizes the outcomes of focus group discussions and participatory research approach sessions held with communities in the villages that are affected by rainfall variability. The article compares the outcomes of the case studies and identifies the similarities and areas of overlap. It concludes that for some households – regardless of the case study – there is high potential for migration to be a successful adaptation strategy. Some other households rather find it hard to adapt to the situation in situ; among them, some cannot afford moving to other areas to improve their livelihoods and remain 'trapped' while others do move, but barely survive or are even subject to worse conditions. The article provides policy recommendations for policy-makers and practitioners that might be applicable for these, and also other countries exposed to the same climatic issues. Finally, the article provides an outlook with lessons learned for the benefit of future research.


Managerial changes are necessary for companies in the Dutch food industry and agribusiness to lessen the environmental impact of their activities. To identify the opportunities or limits of environmental management systems (EMSs), it is important to first understand what influence stakeholders have on EMS development. In an empirical research we found that developmental levels of internally oriented EMSs, which primarily aim at internal administrative procedures, are explained mainly by the frequency of contacts with governmental authorities. For this kind of EMSs, non-commercial stakeholder groups have a major influence on the corporate environmental policy. Externally oriented EMSs, which focus on joint efforts in supply chains, are influenced by commercial groups in the business network (like suppliers, clients and competitors). The development of externally oriented EMSs requires adjustments in the composition of and interaction with the stakeholder environment by governmental agencies as well as managers in the agri-food sector. Opening up towards the stakeholder environment, by (among others) an adjustment of the information system, integrated (supply-chain wide) auditing and licensing, can enhance cost-efficiency, transparency and sustainability.


The present thesis comprises two rather independent chapters. In general, the diagnosis
and quantification of dependence is a major aim of econometric studies. Along these lines,
the concept of dependence serves as an encompassing framework to analyze time series
with two very different techniques.
First, we consider a single macroeconomic time series. A series which incorporates
only temporary deviations from deterministic terms provides a different starting point
for economic interpretations than an 'unpredictable' (random) series. In the context of
dependency, we are interested if a time series is stationary or if it exhibits persistent
dependence on larger horizons. We consider univariate tests for stationarity under distinct
model settings. More recently, panel unit root tests have been developed to overcome
power deficiencies and provide a more general economic statement. The standard panel
unit root tests are not robust under time-varying variances and trending data. Against
this background, we introduce a new test procedure which performs well in this setting.
Departing from the framework of a single time series the diagnosis of dependencies
between several variables provides evidence on relations in a macroeconomic system. As-
suming stationarity of the series, we analyze instantaneous and persistent effects between
macroeconomic indices by means of vector autoregressive models. Dependencies can, be-
yond the standard linear setting, be present in diverse forms. We first refer to the variety
of dependencies. Subsequently, we review and compare nonparametric measures which are
developed to robustly diagnose various dependence types. Drawing on these dependence
diagnostics helps to conduct a preliminary analysis of the data. In macroeconomics, the
analyst might be further interested in causalities. We analyze causalities by means of struc-
tural vector autoregressive models tracing the variables back to unanticipated independent
shocks. Hereby, we are mainly interested in the identification of instantaneous response ma-
trices relying on non-Gaussianity of the structural shocks. We compare such independence
based identification procedures relying on beforehand selected nonparametric dependence
measures. Furthermore, we highlight their performance by means of a simulation study.
The assumption of at most one Gaussian (structural) shocks is essential for unique identi-
fication of the instantaneous response to independent shocks. However, in a system with
multiple Gaussian shocks the non-Gaussian ones can still be uniquely identified. We prove
this result and, thereby, enable a more general definition of identifiability.


This literature review looks at the way in which the researcher–practitioner relationship is described in research publications. The main finding of this review points to: a limited description and discussion of the relationship; a similarly limited, sometimes confusing, understanding of the notion of collaboration; as well as limited self‐reflection, sometimes accompanied by taken‐for‐granted attitudes on the part of the researchers.


Lymphocyte-mediated cytotoxicity has been proposed to consist of the polarized secretion of granule-stored perforin leading to target-cell lysis. Nevertheless, perforin-independent pathways were postulated to explain the cytolytic activity of apparently perforin-free lymphocytes and the DNA degradation found in dying target cells. To evaluate the role of perforin, we used gene targeting in embryonic stem cells to produce mice lacking perforin. Mice homozygous for the disrupted gene have no perforin mRNA. The mice are healthy. Activation and granzyme A secretion of perforin-free cytolytic T cells are unaltered. The killing activity of cytolytic T cells as well as natural killer (NK) cells, however, is impaired but not abolished. Approximately one-third of the killing activity remains when lysis of 3T3 fibroblast targets and the apoptotic cell death of YAC-1 NK targets are analyzed. We conclude that perforin is a crucial effector molecule in T cell- and NK cell-mediated cytolysis. However, alternative perforin-independent lytic mechanisms also exist.


Donald Haase has described how "Sleeping Beauty" has an "underlying preoccupation with the creative power of language and storytelling," but the story, in its various incarnations, has also become an exercise in visualization, giving us instructions on how to construct the image of a beautiful woman, one to be looked at, to use Laura Mulvey's terminology. Over the centuries a once robust narrative with many twists and turns was edited down by the Brothers Grimm to emphasize the enraptured gaze of a rescuing prince as he beholds a woman who, through a sleight of words, becomes a verbal icon. It took Angela Carter to demythologize sleeping beauties in all their verbal and visual variety. In "The Lady of the House of Love," she reveals the dangers of fetishizing not just sleeping beauties but fairy tales in general.


This essay responds to Martin Chanock's argument that race tainted the entire enterprise of South African judging. It seeks to understand how that could have been so, and looks to such driving forces as whites' guilt, denial, identity-building, self-protection, and legitimation for explanations. Then it asks whether an institution so tainted should now be altogether abandoned as part of the rebuilding of post-apartheid South Africa. The essay answers that much should be changed, but that the existence of a judiciary laying claim to a special expertise and responsibility in interpreting law and protecting rights - a key heritage of the old South Africa's judicial system - is an important safeguard of liberty and should not be sacrificed.


Simulated distributions of the chlorofluorocarbons CFC-11 and CFC-12 are used to examine the ventilation of the North Atlantic Ocean in a global version of the Miami Isopycnic Coordinate Ocean Model (MICOM). Three simulations are performed: one with a diapycnal diffusivity Kd= 3 × 10−7/N m2 s−1 and an isopycnal diffusive velocity (i.e., diffusivity divided by the size of the grid cell)vtrac= 0.01 m s−1 (Exp. 1); Exp. 2 is as Exp. 1 but with Kd= 5 × 10−8/N m2 s−1 plus increased bottom mixing; and Exp. 3 is as Exp. 2 but with vtrac= 0.0025 m s−1. The main features of the simulated ventilation are strong uptake of the CFCs in the Labrador, Irminger and Nordic Seas, and a topographically aligned geostrophically controlled southward transport of CFC-enriched water in the Atlantic. It is found that the Overflow Waters (OW) from the Nordic Seas, the penetration of the western boundary currents, the ventilation of the subtropical surface waters, the vertical density stratification and the meridional overturning are all critically dependent on the applied isopycnal and diapycnal diffusivities, with Exp. 3 (Exp. 1) yielding the most (least) realistic results. Furthermore, it is the combined rather than the isolated effect of the isopycnal and diapycnal diffusivities that matter. For instance, the strength of the simulated Meridional Overturning Circulation (MOC) is similar in Exps. 1 and 3, but the simulated CFC-distributions are far too diffusive in Exp. 1 and fairly realistic in Exp. 3. It is demonstrated that the simulated distributions of transient tracers like the CFCs can be used to set the strength of the applied isopycnal mixing parameterization, a task that is difficult to conduct based on the simulated hydrography alone.


We implement panel data econometrics on non-linear empirical models to investigate how the firms' market value is related with cash holdings and ownership concentration, on non-financial listed companies in Greece, before (2000-2009) and during the Eurozone crisis 2010-2015. It is confirmed the existence of an optimum level of cash (CASH) and the top 5 major shareholders ownership (OWN5) at which firms' return on equity (ROE) has been maximized (concave function), especially over the crisis period and the total one. Yet, a convex function of the Tobin's Q ratio (Q) on OWN5 has also been revealed significant for all sample periods. The findings support the tradeoff theory and the new kind of agency cost literature on expropriation effects of the minority by the majority. Interaction terms have also been found statistically significant, confirming that the special context of the Eurozone has influenced business, in the narrow Athens Stock Exchange (ASE). The estimated averages that maximize firms' market values (for instance ROE), in relation to either CASH (0.83 of net assets, during the whole sample period 2000-'15, while 0.77 in the crisis one 2010-'15) or OWN5 (0.10 of equity, during 2000-'15, while 0.36 in the crisis one 2010-'15) could be useful for both investors and policy makers in Greece, a member-country of "sui generis†Eurozone, with an unsustainable public debt.


The objective of this paper is to present the feasibility of a self-rechargeable digital water flow meter (SRDFM) system for water pipes using the latest data processing and wireless communication technologies while causing negligible water pressure drop (head loss). The system uses a Pelton turbine generator to power the electronic circuit, which processes and transmits the signals generated by several flow meters. ZigBee technology was used to process and send wireless signals. Signals from two water meters were acquired, processed, and transmitted with only one control/transmission unit during this study. The new system was assessed experimentally, reaching a maximum of 80 m of wireless transmittance distance at a minimum flow rate of 5 L/min for a 16-mm diameter pipe (self-charged).


Merlin.net™ Patient Care Network is a St. Jude Medical product that is used for remote patient management. The basic concept of Merlin.net is to allow the physician to view patient device follow-up information as well as general patient and device information on a web application. The Merlin.net system also interfaces with the patient and will send them notification if they miss a follow-up. All device information will be collected automatically while the patient is sleeping. This information is sent through a telephone line to a Merlin.net server to process a report package and display the collected information on the Merlin.net web application. The Merlin.net verification team ensures that all reports generated by the Merlin.net servers are processed and outputted correctly. There are currently 296 device parameters supported by Merlin.net, and the manual extraction and comparison of the expected parameter values takes several hours for each patient follow-up session. Currently there are 250 patient follow-up sessions used for verification testing. Each new release will continue to create additional patient follow-up sessions. Merlin.net releases are approximately 6 months apart, and each new release adds approximately 30-50 new patient follow-up records to support the new devices. In order to meet aggressive project deadlines, while ensuring that the Merlin.net system is processing and outputting patient follow-up data correctly, it is necessary to come up with an automated process to verify the contents of the processed data is correct. This will save a tremendous amount of time as well as improve on the quality of the v verification process by eliminating human error and rework. It is critical for patient safety that the patient device follow-up information is processed and outputted correctly. In this thesis an automated process was developed to verify the correct content of the Merlin.net server generated reports for each patient follow-up session. This process leveraged different tools and scripting languages to achieve automation. TDE (Test Development Environment) tool was used to extract the device parameters from the patient follow-up sessions. The TDE script was written to extracts the desired parameter values from the patient follow-up session and automatically populates parameters in a device parameters spreadsheet. Once all the device parameter values are extracted in the spreadsheet, they are passed through a set of mapping rules, which form the expected values. The mapping rules were implemented as VBA (Visual Basic for Application) macros, one macro for each report. The VBA macros write …


Seedborne peanut viruses pose important constraints to peanut production and safe movement of germ plasm. They also pose a risk of accidental introduction into previously disease-free regions. We have developed reverse transcription-polymerase chain reaction (RT-PCR) assays based on identical cycling parameters which identified peanut stripe, Peanut mottle, Peanut stunt, and Cucumber mosaic viruses through production of specific DNA fragments of 234 bp, 327 bp, 390 bp, and 133 bp, respectively. Assay sensitivity in the picogram range was achieved. The two potyviruses and two cucumoviruses could be differentiated using duplex RT-PCR assays. These assays should be useful for testing peanut leaves or seeds for virus identification in epidemiological studies, seed testing or in post-entry quarantine.


This article examines globalized knowledge flows through a case study of the treatment of the Chinese concept of guanxi in social and management science journals. Three forms of concept–theory relations are postulated which effectively correspond with different patterns of knowledge flow. The treatment of this concept in 214 refereed journal articles published from 1999 to 2009 indicates that the concept guanxi, which possesses capacity for theoretical development, is predominantly treated merely as an object of study rather than the basis of theoretical elaboration. A continuing dominant pattern of knowledge flows from the metropole to the periphery is thus indicated. The mechanisms of continuing asymmetrical knowledge flow are discussed, including language used, site of publication, channel of transmission and method as factors generating this outcome.


Subtypes of breast cancer that represent the two major types of epithelial cells in the breast (luminal and basal) carry distinct histopathologic profiles. Breast cancers of the basal-like subtype, which include the majority of hereditary breast cancers due to mutations in the breast cancer susceptibility gene 1 (BRCA1), frequently assume triple-negative status, i.e., they lack expression of estrogen receptor-alpha and progesterone receptor, and lack overexpression or amplification of the HER2/NEU oncogene. Defects in DNA damage response pathways result in genome instability and lead to carcinogenesis, but may also be exploited for therapeutic purposes. We analyzed repair of oxidative DNA damage by the base-excision repair (BER) pathway, which when aberrant leads to genomic instability and breast carcinogenesis, in cell lines that represent the different subtypes of breast cancer and in the presence of BRCA1 deficiency. We found that basal-like and BRCA1-mutated breast cancer cells were defective in BER of oxidative DNA damage, and that this defect conferred sensitivity to inhibition of poly(ADP-ribose) polymerase, a DNA repair enzyme. The defect may be attributed, at least in part, to a novel role for BRCA1 in the BER pathway. Overall, these data offer preventive, prognostic, and therapeutic usefulness.


We have developed an efficient methodology for Bayesian prediction of lithology and pore fluid, and layer-bounding horizons, in which we include and use spatial geologic prior knowledge such as vertical ordering of stratigraphic layers, possible lithologies and fluids within each stratigraphic layer, and layer thicknesses. The solution includes probabilities for lithologies and fluids and horizons and their associated uncertainties. The computational cost related to the inversion of large-scale, spatially coupled models is a severe challenge. Our approach is to evaluate all possible lithology and fluid configurations within a local neighborhood around each sample point and combine these into a consistent result for the complete trace. We use a one-step nonstationary Markov prior model for lithology and fluid probabilities. This enables prediction of horizon times, which we couple laterally to decrease the uncertainty. We have tested the algorithm on a synthetic case, in which we compare the inverted lithology and fluid probabilities to results from other algorithms. We have also run the algorithm on a real case, in which we find that we can make high-resolution predictions of horizons, even for horizons within tuning distance from each other. The methodology gives accurate predictions and has a performance making it suitable for full-field inversions.


This study examined the contribution of frequency-dependent short-term depression of PSP amplitude to low-pass temporal filtering in the weakly electric fish Eigenmannia. Behavioral and neurophysiological methods were used. Decelerations of the electric organ discharge frequency were measured in response to continuous and discontinuous electrosensory stimuli. Decelerations were strongest (median = 4.7 Hz; range, 3.5–5.9 Hz) at continuous beat rates of ∼5 Hz and weakest (median = 0.4 Hz; range, 0.0–0.8 Hz) at beat rates of 30 Hz. Gating 20 or 30 Hz stimuli at a rate of 5 Hz, however, elicited decelerations that were sixfold greater than that of continuous stimuli at these beat rates (median = 2.6 Hz; range, 2.0–4.7 Hz for 30 Hz). These results support the hypothesis that short-term processes enhance low-pass filtering by reducing responses to fast beat rates. This hypothesis was tested by recording intracellularly the responses of 33 midbrain neurons to continuous and discontinuous stimuli. Results indicate that short-term depression of PSP amplitude primarily accounts for the steady-state low-pass filtering of these neurons beyond that contributed by their passive and active membrane properties. Previous results demonstrate that passive properties can contribute up to 7 dB of low-pass filtering; PSP depression can add up to an additional 12.5 dB (median = 4.5). PSP depression increased in magnitude with stimulus frequency and showed a prominent short-term component (t1 = 66 msec at 30 Hz). Initial PSP amplitude recovered fully after a gap of 150 msec for most neurons. Remarkably, recovery of PSP amplitude could be produced by inserting a brief low–temporal frequency component in the stimulus.


We present a new approach of generating the several wavelengths required for color holography with coherence lengths in the range of several meters. Our proposed laser system consists of an argon-ion laser, which is equipped with broadband optics. Its main lasing lines include 457, 488, and 514 nm. Sufficient coherence length is achieved by means of an intracavity etalon. We report single-frequency operation at several competing wavelengths and the successful recording of multicolor holograms with the described laser system.


Abstract. PREVIEW is an European Commission FP6 Integrated Project with the aim of developing, at an European level, innovative geo-information services for atmospheric, geophysical and man-made risks. Within this framework, the Landslides Platform Service 2 (forecasting of shallow rapid slope movements) has developed an integrated procedure for the forecasting and warning of distributed shallow landsliding to be used for civil protection purposes. The Service consists of an automated end-to-end forecasting chain which uses data from a probabilistic downscaled short-term rainfall forecast, soil saturation estimates and meteorological radar outputs. The above data are entered into a hydro-geological model that makes use of an infinite slope approach to calculate the distributed Factor of Safety over the entire basin. All outputs, and much of the input data, are shown on a WebGIS system so that end-users can interactively access and download data. A distinctive feature of the service is the use of an innovative soil depth model for predicting the distributed thickness of the regolith cover within the basin, which is one of the most important parameters controlling shallow landslide triggering. The service was developed in a pilot test site in NE Italy, the Armea basin. Validation makes use of two rainfall events: one that occurred in 2000 and a smaller, more recent event (2006) that caused fewer landslides. Rainfall data have been used to compute a distributed factor-of-safety map that has been overlaid onto the landslide inventory. Instead of a traditional validation approach based on the number count of correctly identified landslides, we carried out an alternative procedure based on the landslides area that gave outcomes which, for this preliminary stage of the research, can be considered promising.


This paper analyzes how professional values and practices influence the character of nonprofit organizations, with data from a random sample of 501 (c)(3) operating charities in the San Francisco Bay Area collected between 2003 and 2004. Expanded professionalism in the nonprofit world involves not only paid, full-time careers and credentialed expertise but also the integration of professional ideals into the everyday world of charitable work. We develop key indicators of professionalism and measure organizational rationalization as expressed in the use of strategic planning, independent financial audits, quantitative program evaluation, and consultants. As hypothesized, charities operated by paid personnel and full-time management show higher levels of rationalization. While traditional professionals (doctors, lawyers, and the clergy) do not differ significantly from executives with no credentialed background in eschewing business-like practices, managerial professionals champion such efforts actively, as do semi-professionals, albeit more modestly. Management training is also an important spur to rationalization. We assess what is gained and lost and the tension that can arise when nonprofits become professionalized and adopt more methodical, bureaucratic procedures.


The digital scholarly editions that textual scholars and digital humanists produced for over two decades have often been accompanied by claims about their revolutionary nature. Technological innovations are prone to induce such claims, as the case of the compact disc audio carrier exemplifies. However, on closer inspection we are hard pressed to identify the revolution that such innovations bring about. Arguments that textual scholars have put forward to claim fundamental differences between print and digital scholarly editions turn out to be weak. Some, therefore, claim that in fact no essential changes exist between print and digital text, nor between print or digital textual scholarship. However, none of the arguments takes into account the most striking difference between print and digital text, which is the performative potential of the latter. Digital text is endowed with a performative aspect through software code. However, current digital scholarly editions adhere almost exclusively to a representational philosophy in which the idea of text as a static materialized object is unaltered digitally remediated, that is: they are mostly static digital objects mimicking static print books. Perceived primarily as infrastructure underpinning digital scholarly editions, executable program code has until now largely been ignored by textual scholarship as a methodological means. Code, as will be explained, is the very embodiment of the ontological difference between print and digital text—it is text performing. This ontological shift and the emergence of a performative digital textual heritage do justify explorations of the methodological affordances that code creates for textual scholarship. As an example of such an exploration the idea of the computational scholarly edition is introduced (as opposed to the "merely digital" scholarly edition) and a tentative implementation is presented. Finally it is argued that, rather than to extradite them, it is more productive to embrace digital and computational exploratory niches in textual scholarship.


There is no question that the Arctic is particularly affected by climate change as was shown not only in IPCCs recent 4th Assessment Report, but also specified in the Arctic Climate Impact Assessment (ACIA) and Arctic Human Development Report (AHDR) as well as in the Science Plans of the Second International Conference of Arctic Research Planning (ICARP II). Of particular interest is the response of geophysical and ecosystem features in the Arctic coastal zone to effects of global warming, i.e., the decreasing sea ice cover, the destabilization of permafrost systems and increased exposure of the coast to storms. Embedded in a coupled socio-ecological context are issues of societal response to these changes. This includes both adaptation to changing living conditions bearing threats and options for human welfare as well as new forms of land and sea use such as enhanced access to and exploitation of resources, or increasing ship traffic.Despite heightened media coverage and imminent changes to the coastal zone environment in the Arctic, little is known about the actual evolution of the shoreline and related socio-economical and ecological impacts. This paper provides a rapid overview of the current knowledge on arctic coastal dynamics and the gaps that new programs initiated during the IPY are attempting to fill.


Deployable structures are structures that transform their shape from a compact state to an extended in-service position. Structures composed of tension elements that surround compression elements in equilibrium are called tensegrity structures. Tensegrities are good candidates for deployable structures since shape transformations occur by changing lengths of elements at low energy costs. Although the tensegrity concept was first introduced in 1948, few full-scale tensegrity-based structures have been built. Previous work has demonstrated that a tensegrity ring topology is potentially a viable system for a deployable footbridge. This paper describes a study of a near-full-scale deployable tensegrity footbridge. The study has been carried out both numerically and experimentally. The deployment of two modules (one half of the footbridge) is achieved through changing the length of five active cables. Deployment is aided by energy stored in low stiffness spring elements. Self-weight significantly influences deployment, and deployment is not reproducible using the same sequence of cable- length changes. Active control is thus required for accurate positioning of front nodes in order to complete deployment through joining both sides at center span. Additionally, testing and numerical analyses have revealed that the deployment behavior of the structure is nonlinear with respect to cable-length changes. Finally, modeling the behavior of the structure cannot be done accurately using friction-free and dimension- less joints. Similar deployable tensegrity structures of class two and higher are expected to require simulation models that include joint dimensions for accurate prediction of nodal positions. DOI: 10.1061/(ASCE)ST.1943-541X.0001260 . © 2015 American Society of Civil Engineers.


Women present with a big mass will feel worried and uncertain about herself as there is a possibility of cancer. Most of the big masses are originated from the uterus or ovarian origin and it is vital for the patient to find out the most possible diagnosis. With the improvement of modern technology, the ultrasound plays an important role to get the diagnosis, however, it has some limitations like difficulty to visualize the whole mass. Other investigations like CT and especially, MRI will help to reach the appropriate diagnosis and as a result, aid in making an efficient plan for the respective diagnosis with least morbidity. It is a great challenge for the clinician but those who have adequate knowledge, competency in surgical skill with well-learnt experience will not consider it as a big deal.


This paper analyses whether the different powers and resources at the
disposal of local and regional governments across Europe deliver greater
satisfaction with political institutions and lead to greater personal happiness.
The analysis uses microdata from the four available waves of the European
social survey (2002, 2004, 2006 and 2008), including more than 160,000
observations of individuals living in 29 European countries. Our results
reveal that political and fiscal decentralization have a positive and
significant effect on individuals' overall happiness. Fiscal decentralization
also exerts a significant effect on the level of satisfaction with political and
economic institutions and with the education and health systems, whereas
the effect of political decentralization on these variables is more limited.
The results show that citizens seem to be happier with the actual capacity of
their local governments to deliver than with the general principle that they
can have a say on their daily politics and policies.
Keywords: Happiness, well-being, satisfaction, fiscal and political
decentralization, Europe.
JEL codes: H11, H77


Arginine vasopressin (AVP) and corticotrophin‐releasing hormone (CRH) in the parvocellular neurosecretory cells of the paraventricular nucleus (PVN) play a major role in activating the hypothalamic‐pituitary‐adrenal axis, which is the main neuroendocrine response against the many kinds of stress. We examined the effects of chronic inflammatory/nociceptive stress on the expression of the AVP‐enhanced green fluorescent protein (eGFP) fusion gene in the hypothalamus, using the adjuvant arthritis (AA) model. To induce AA, the AVP‐eGFP rats were intracutaneously injected heat‐killed Mycobacterium butyricum (1 mg/rat) in paraffin liquid at the base of their tails. We measured AVP, oxytocin and corticosterone levels in plasma and changes in eGFP and CRH mRNA in the hypothalamus during the time course of AA development. Then, we examined eGFP fluorescence in the PVN, the supraoptic nucleus (SON), median eminence (ME) and posterior pituitary gland (PP) when AA was established. The plasma concentrations of AVP, oxytocin and corticosterone were significantly increased on days 15 and 22 in AA rats, without affecting the plasma osmolality and sodium. Although CRH mRNA levels in the PVN were significantly decreased, eGFP mRNA levels in the PVN and the SON were significantly increased on days 15 and 22 in AA rats. The eGFP fluorescence in the SON, the PVN, internal and external layers of the ME and PP was apparently increased in AA compared to control rats. These results suggest that the increases in the concentrations of ACTH and corticosterone in AA rats are induced by hypothalamic AVP, based on data from AVP‐eGFP transgenic rats.


Insisting on the critical value of Latin American histories for recasting theories of postcolonialism, After Spanish Rule is the first collection of essays by Latin Americanist historians and anthropologists to engage postcolonial debates from the perspective of the Americas. These essays extend and revise the insights of postcolonial studies in diverse Latin American contexts, ranging from the narratives of eighteenth-century travelers and clerics in the region to the status of indigenous intellectuals in present-day Colombia. The editors argue that the construction of an array of singular histories at the intersection of particular colonialisms and nationalisms must become the critical project of postcolonial history-writing.

Challenging the universalizing tendencies of postcolonial theory as it has developed in the Anglophone academy, the contributors are attentive to the crucial ways in which the histories of Latin American countries—with their creole elites, hybrid middle classes, subordinated ethnic groups, and complicated historical relationships with Spain and the United States—differ from those of other former colonies in the southern hemisphere. Yet, while acknowledging such differences, the volume suggests a host of provocative, critical connections to colonial and postcolonial histories around the world.

Contributors

Thomas Abercrombie

Shahid Amin

Jorge Canizares-Esguerra

Peter Guardino

Andres Guerrero

Marixa Lasso

Javier Morillo-Alicea

Joanne Rappaport

Mauricio Tenorio-Trillo

Mark Thurner


A 75 cm OD x 33 mm thick pipe in a horizontal section of a hot steam reheat line ruptured after 15 years in service. The failed section was manufactured from rolled plate of material specification SA387, grade C. The longitudinal seam weld was a double butt-weld that was V-welded from both sides and failure was found to propagate along the longitudinal seam and its HAZ. The fracture surface near the inner wall of the pipe was found to have a bluish gray appearance, while the fracture surface near the outer wall was rust colored (oxides). The transverse-to-the-weld specimen from the longitudinal seam weld was revealed to have lower elongation and a shear type failure rather than the cup-cone failures. It was concluded that the welded longitudinal seam exhibited embrittlement. A low-ductility intergranular fracture that progressed through the weld metal was revealed by scanning electron microscopy. The cracks were revealed to be in existence for some time before the final failure which was indicated by the extent and amount of corrosion products. It was concluded that low ductility was responsible for the original initiation of cracks in the pipe.


A systemic functional analysis of the oral pedagogical discourse and board texts of secondary school mathematics lessons differentiated on the basis of school sector, gender and social class is completed through the development of a computer program to handle the linguistic analysis and the construction of a Hallidayan systemic framework for mathematical symbolism and visual depiction. The new frameworks allow for investigation of the unique contributions of language, mathematical symbolism and visual display in the construction of meaning in mathematical texts and the process of semiotic metaphor which occurs in movements between these codes. The systemic analysis of the classroom discourse is situated within a Foucauldian perspective of power, knowledge and truth in mathematics, mathematics education and wider discursive practices involving the private and state school sectors.

The analysis of linguistic patterns, register selections and genres of four Year Ten secondary school mathematics lessons reveals that in private elite single sex schools the male students demonstrate the greatest participation and access to the discourse of mathematics while the female students participate in interpersonal patterns of deference which do not resonate with the tenor dimensions of mathematics. The monofunctional tendency orientated towards interpersonal meaning in the lesson of the working class students at a government school indicates that the social goal of the lesson is primarily directed towards maintaining tenor relations through covert manipulation as opposed to learning mathematics. The limited functionality of practical lessons in mathematics is also demonstrated as a shift from everyday discourse to mathematical discourse does not occur. Mathematical pedagogical discourse is characterised by a dense texture which arises in part from the strategies by which meaning is encoded in mathematical symbolism. As opposed to the lexical density and grammatical intricacy of written and spoken language respectively, mathematical symbolism realises grammatical density whereby multiple levels of clausal rankshift preserve the nuclear configurations of Operative processes and participants which describe relations of parts to the whole and continuous patterns of variation. In addition, inherent difficulties in mathematical pedagogical discourse arise from long implication chains of reasoning and dependence on multiple semiotic resources with the latter resulting in referential complexity and iii multisemiotic intertwining of lexical and participant chains and strings. The results of the analysis, interpreted through Bernstein's theory of pedagogical practices and coding orientations and Halliday's formulations of spoken and written language, reveal that the semantic orientation of working class students does not accord with that realised in mathematics.


In this paper, we investigate the possible impact of crowdsourcing on organisational practices. We answer the research question of whether and to what extent the practices of crowdmapping impact humanitarian organisations. To answer this question, we examine a crowdmapping initiative during a natural disaster. The data collection is based on forty interviews with different actors including crowdmappers, humanitarian organisations, government specialists and technology providers. Concepts from structuration theory are applied to conceptualise and make sense of the data. The findings reveal the process of change that took place in the practices of a humanitarian organisation. They also show that these changes recursively impacted the practices of crowdmapping. We then argue that there is a duality of change between the micro-practices of crowdmapping and the macro-practices of a humanitarian organisation. The implications of the study on research and practice are then discussed.


The purpose of this volume is to shape conceptual tools to understand the impact of new information and communication technologies (ICTs) on the organization of universities. Traditional research-based universities, the most typical representatives of the higher education system, find themselves challenged by the speed and the wide range of technical innovations, but also by a vast array of implicit assumptions and explicit promises associated with the distribution of digital media. The author observes that as universities increasingly use digital media (computers and the Internet) to accomplish their tasks, a transformation takes place in an evolutionary rather than in a revolutionary way. Using the University of Klagenfurt as an in-depth case study, he explores such dynamic issues as how digital media affect the practice of research, the preservation and dissemination of knowledge (for example, through publishing and archiving), and delivery of education at universities. More broadly, he considers issues of organizational culture and design, administration, and leadership as universities integrate digital technologies into all aspects of their operations.


Economics has the reputation to be an insular discipline with little consideration for other social sciences and humanities (SSH). New research [Angrist et al, 2020, JEL] challenges this perception of economics: the perception would be historically inaccurate and especially at odds with the recent interdisciplinarity of economics. By systematically studying citation patterns since the 1950 in thousands of journals, we offer the best established conclusions to date on this issue. Our results do show that the discipline is uniquely insular from a historical point of view. But we also document an important turn after the 1990s that drastically transformed the discipline has it became more open, very quickly, to the influence of management, environmental sciences, and to a lesser degree, a variety of SSH. While this turn made economics less uniquely insular, as of today economics remains the least outward-looking discipline with management among all SSH. Furthermore, unlike in the other major social sciences, the most influential journals in economics have not significantly contributed to the recent increase in the interdisciplinarity of the discipline. While economics is changing, it is too soon to claim that it has completed an interdisciplinary turn.


This article investigates the importance of using draft curtains to obtain faster sprinkler activation with three different models-two computational fluid dynamics (CFD) models (CFX 4.4 and fire dynamics simulator (FDS) 4.07) and a zone model (Argos) containing a ceiling-jet formula - for an actual scenario in an entertainment center in Denmark. It is found that a draft curtain has some effect on sprinkler activation, reducing activation time from 8% to 15%, depending on the model implemented. The positions of the sprinklers with in the vertical computational grid of the CFD simulations have a greater influence on the activation of the sprinkler, where FDS is more sensitive than CFX. It is confirmed that heat transfer from the ceiling jet to the ceiling has little influence on the results. The zone model with a ceiling-jet formula gives 10—20% slower sprinkler activation than the CFD results when the sprinkler is close to the ceiling, but is still considered very useful in view of the faster calculation time.


The Campanian Desert Member and Lower Castlegate Sandstone in the Book Cliffs of east-central Utah to western Colorado, USA, has served as a foundational data set in the development of sequence stratigraphy. Contrary to previous work, no third-order sequence boundaries are recognized. These were originally thought to partition the neighboring coastal plain and shallow marine facies belts into separate systems tracts, unlinked in time or space. In contrast, adjoining channel-coastal plain and shallow marine facies belts are genetically-, temporally-, and spatially-related. Evidence includes the (i) synchronous, strongly progradational stacking patterns within each facies belt, (ii) gradational and conformable transitions between adjoining facies belts, accentuated by the ubiquity of flat-topped, rooted foreshore sandstones passing upwards into carbonaceous-rich-mudstone-dominated coastal plain, (iii) parasequence-scale interfingering of coastal plain-channel and foreshore-shoreface deposits, with channels, white caps and coals embedded within stacked shoreface parasequences, (iv) regional correlation of coals and flooding surfaces, and (v) near orthogonal paleocurrent relationship between channels and shorelines. Terminal channels incise into proximal foreshore-shoreface sandstones in most Desert-Castlegate parasequences. Incisions are generally confined to the parasequence in which the channels are nested, rarely cutting deeper. These shoreface-incised channels are cut and filled at a parasequence-scale, and are bounded above by the same flooding surface that caps each foreshore-shoreface package. The ubiquity of ascending regressive shoreface trajectories and near absence of descending regressive trajectories that intersect depositional slope argues against any significant sea level fall. Increased rates of sediment supply, driven by autogenic and/or allogenic processes, likely generated the strongly progradational Desert-Castlegate great tongue of sandstone.


During the decade of the Collaborative Research Center CRC 570 "Distortion Engineering" many methods were developed and extended. Within this paper these methods are evaluated in respect of practical application in process planning. The methods can be arranged by two different targets. On the one hand by mainly consider distortion up to predict distortion to support planning. The similarity of all methods is given due to the fact that distortion has to be seen as a system attribute. After the introduction of the methods and their basic functionality they were evaluated against process planning in particular the planning types "repeat planning" up to "new planning". Generally it can be shown that the transferability on other geometries, processes and materials is strongly limited. Furthermore the methods which only consider distortion seem better to fulfill the tasks of the planning types as methods which determine distortion. The best method was the method which compares different process chains. Nevertheless, this more theoretical evaluation has to be verified by industrial process planning.


In the course of the later years a rather large number of specimens of various groups of insects from New Guinea were among the acquisitions of the Rijksmuseum van Natuurlijke Historie. Shortly after the war the collections made by Professor H. Boschma, during his visit to the Wissel Lakes in 1939 as the zoologist of the expedition organized by the Royal Netherlands Geographical Society (Koninklijk Nederlandsch Aardrijkskundig Genootschap), arrived safely from Bogor (Java) where they had to stay during the German occupation of the Netherlands. Quite recently we obtained the first captures made by Dr. L. D. Brongersma in various places in and near New Guinea. From both collections a number of new and interesting Heteroptera is to be described. A few Oncomerini, belonging to the genera Lyramorpha and Agapophyta, are the subject of the present note. Lyramorpha Subgenus Lyrodes Of the subgenus Lyrodes two new species were collected at and near the Wissel Lakes. The first, which I will name Lyramorpha (Lyrodes) edulis nov. spec.*) (figs. 1-2), shows no definite spot or patch on the corium; the basal corners of the scutellum are yellowish, medially from this yellow spot a distinct dark point is to be seen, except in specimens that are more or less immature. The general colour is more or less castaneous, with inVentral views of the ultimate abdominal segments of: fig. I, Lyramorpha (Lyrodes) edulis ♂ ; fig. 2, Lyramorpha (Lyrodes) edulis ♀ ; fig. 3, Lyramorpha (Lyrodes) plagifer ♂ ; fig. 4, Lyramorpha (Lyrodes) plagifer ♀ ; fig. 5, Lyramorpha (Diploxiphus) maculifer ♂ ; fig. 6, Lyramorpha (Diploxiphus) maculifer ♀.


In this Practice article by Sheikha Al-Jabri and colleagues (BMJ 2010;341:c3770, doi:10.1136/bmj.c3770), the authors confirm that they made errors in the references. Reference 8 in the reference list was wrong and should not have been cited. The third paragraph of the "investigations" section (in which reference 8 was cited) should have been referenced as follows (note that the numbering here does not relate to the published numbering): "In primary care, transvaginal ultrasound may not be readily available and transabdominal ultrasound is considered a useful screening test for early pregnancy complications, with a sensitivity of 80% and specificity of 78%. Finding an intrauterine gestation on abdominal scan effectively excludes the possibility of an ectopic pregnancy. However, ultrasound diagnosis should bemade by visualising an adnexal mass rather than the absence of intrauterine sac only. For more definitive diagnosis, the sensitivity of transvaginal ultrasound to diagnose tubal ectopic pregnancy is 90.9% and the specificity is 99.9%."


A molecular description of the mesomorphic behaviour of globular supermolecules of radial dendritic architecture is presented. It is based on coarse grained structural and conformational features, namely, anisometry of the overall shape, submolecular partitioning of the interactions and non-convexity of the supermolecular surface. The relevance of these features to conformational phase transitions, orientational ordering, layering and microsegregation phenomena in ordered fluid phases is studied using density functional theory of deformable bodies and Monte Carlo simulations of rigid fan molecules. Orientationally ordered and layered phases are found, both in theory and simulation, even in the absence of significant overall shape anisometry.


The ASTM C457 test has long been a standard used to obtain the air void parameters of concrete materials. These air void parameters provide valuable information that has been linked to the performance of concrete under conditions such as freezing and thawing cycles. The standard test procedure involves linearly traversing a cut and polished section of a concrete specimen while a technician observes it under a microscope. Chord lengths of material constituents that the technician observes along the linear traverse are recorded and later used to calculate air void parameters statistically. This procedure is long and tedious, which makes it susceptible to human error due to operator fatigue. This study proposes and implements a new test method for evaluating concrete air void parameters using an image analysis method. A polishing procedure along with a differential interference contrast microscope are used to obtain high contrast images of material constituents, which provide raw data for the image analysis method. Because of the high contrast that can be obtained, cement paste, air voids in the cement paste, and aggregate materials in the concrete can be distinguished from one another based on these images. An image analysis program has been written for this study which linearly traverses these images and records the chord lengths of material constituents in a similar way to the standard ASTM C457 test. The chord length data must be processed further, however, because features in the images can be truncated by the edge of the image. Correction calculations for this problem are implemented in the image analysis algorithm. Two specimens which have been previously tested using the standard ASTM C457 method by the Virginia Transportation Research Council, (VTRC), are used in this study. The air void parameters obtained using the new test are compared directly with the results obtained by VTRC for the two specimens. Statistical comparisons indicate that the results of the new test are indeed significant, showing the potential it has for practical implementation. There are drawbacks to the test including a long polishing procedure, but this process can be automated. The new test appears to have excellent potential for practical application, but it should be emphasized that the test has only been implemented using materials in two concrete specimens. Further study on a variety of other concrete materials would be required for implementation in a standard procedure.


Laser Doppler velocimetry (LDV) and video flow visualization are used to investigate the creeping motion of a highly elastic, constant-viscosity fluid flowing past a cylinder mounted centrally in a rectangular channel. A sequence of viscoelastic flow transitions are documented as the volumetric flow rate past the cylinder is increased and elastic effects in the fluid become increasingly important. Velocity profiles clearly show that elasticity has almost no effect on the kinematics upstream of the cylinder, but that the streamlines in the wake of the cylinder are gradually shifted further downstream. Finite element calculations with a nonlinear constitutive model closely reproduce the evolution of the steady two-dimensional velocity field. However, at a well defined set of flow conditions the steady planar stagnation ow in the downstream wake is experimentally observed to become unstable to a steady, three-dimensional cellular structure. The Reynolds number at the onset of the flow instability is less than 0.05 and inertia plays little role in the flow transition, LDV measurements in the wake close to the cylinder reveal large spatially periodic fluctuations of the streamwise velocity that extend along the length of the cylinder and more than five cylinder radii downstream of the cylinder. Fourier analysis shows that the characteristic spatial wavelength of these flow perturbations scales closely with the cylinder radius R. Flow visualization combined with LDV measurements also indicates that the perturbations in the velocity field are confined to the narrow region of strongly extensional flow near the downstream stagnation point. A second flow transition is observed at higher flow rates that leads to steady translation of the cellular structure along the length of the cylinder and time-dependent velocity oscillations in the wake. Measurements of the flow instability are presented for a range of cylinder sizes, and a stability diagram is constructed which shows that the onset point of the wake instability depends on both the extensional deformation of the fluid in the stagnation flow and the shearing flow between the cylinder and the channel.


According to David Hume our idea of a necessary connection between what we call cause and effect is produced when repeated observation of the conjunction of two events determines the mind to consider one upon the appearance of the other. No matter how we interpret Hume's theory of causation this explanation of the genesis of the idea of necessity is fraught with difficulty. I hope to show, looking at the three major interpretations of Hume's causal theory, that his account is contradictory, plainly wrong, or (at best) inherently impossible to verify.


We present a framework for automatically learning human user models from joint-action demonstrations that enables a robot to compute a robust policy for a collaborative task with a human. First, the demonstrated action sequences are clustered into different human types using an unsupervised learning algorithm. A reward function is then learned for each type through the employment of an inverse reinforcement learning algorithm. The learned model is then incorporated into a mixed-observability Markov decision process (MOMDP) formulation, wherein the human type is a partially observable variable. With this framework, we can infer online the human type of a new user that was not included in the training set, and can compute a policy for the robot that will be aligned to the preference of this user. In a human subject experiment (n=30), participants agreed more strongly that the robot anticipated their actions when working with a robot incorporating the proposed framework (p<0.01), compared to manually annotating robot actions. In trials where participants faced difficulty annotating the robot actions to complete the task, the proposed framework significantly improved team efficiency (p <0.01). The robot incorporating the framework was also found to be more responsive to human actions compared to policies computed using a hand-coded reward function by a domain expert (p<0.01). These results indicate that learning human user models from joint-action demonstrations and encoding them in a MOMDP formalism can support effective teaming in human-robot collaborative tasks.


Personally Speaking provides a forum for readers to describe important issues or events in their lives and the role, if any, counseling has played. Guidelines for submitting manuscripts may be obtained from Stephen G. Weinrach, 303 Falvey Hall, Villanova University, Villanova, PA 19085-1699.


We develop a theoretical model with labor market frictions, incomplete financial markets and with households which have two members. Households face unemployment risks but their members adjust their labor supplies to insure against unemployment. We use the model to explain the cyclical properties of aggregate employment and participation. As in the US data, the model predicts that the participation rate (the fraction of individuals that want jobs) is not strongly correlated with aggregate economic activity. This property is in sharp contrast to the strongly procyclical participation predicted by both neoclassical models and models with search frictions, when we assume bachelor households or households with infinitely many members (complete markets). In the two member household model and in the data, primary earners are always in the labor force, secondary earners have a mildly countercyclical participation rate and a mildly procyclical employment rate. Their behavior insures the household against unemployment risks.


ABSTRACT The present paper deals with post-separation child positions in two domains of practice in the Swedish welfare state: welfare benefits such as financial aid and child contact. The area of concern is financial oppression in the context of parental separation. Empirically, the study draws upon three types of material: interviews with victimised mothers, court orders in welfare benefit appeals and court orders in contested contact cases. Findings suggest that financial abuse in the context of parental separation is a non-question in the domain of welfare benefits, and in the domain of child contact framed as a conflict between equal parties. Object without voice and with needs ignored was the typical child position in welfare benefits cases, whereas incompetent subject was more prominent in child contact cases. Age order as a form of domination may be reinforced by the practice of both domains.


Background: Plasmodium, the causative agent of malaria, exports many proteins to the surface of the infected red blood cell (iRBC) in order to modify it toward a structure more suitable for parasite development and survival. One such exported protein, SURFIN4.2, from the parasite of human malignant malaria, P. falciparum, was identified in the trypsin-cleaved protein fraction from the iRBC surface, and is thereby inferred to be exposed on the iRBC surface. SURFIN4.2 also localize to Maurer's clefts—parasite-derived membranous structures established in the RBC cytoplasm and tethered to the RBC membrane—and their role in trafficking suggests that they are a pathway for SURFIN4.2 transport to the iRBC surface. It has not been determined the participation of protein domains and motifs within SURFIN4.2 in transport from Maurer's clefts to the iRBC surface; and herein we examined if the SURFIN4.2 intracellular region containing tryptophan-rich (WR) domain is required for its exposure on the iRBC surface. Results: We generated two transgenic parasite lines which express modified SURFIN4.2, with or without a part of the intracellular region. Both recombinant SURFIN4.2 proteins were exported to Maurer's clefts. However, only SURFIN4.2 possessing the intracellular region was efficiently cleaved by surface treatment of iRBC with proteinase K. Conclusions: These results indicate that SURFIN4.2 is exposed on the iRBC surface and that the intracellular region containing WR domain plays a role on the transport from Maurer's clefts to the iRBC membrane.


Whether a state attorney general has a duty to defend the validity of state law is a complicated question, one that cannot be decided by reference either to the oath state officers must take to support the federal Constitution or the supremacy of federal law. Instead, whether a state attorney general must defend state law turns on her own state's laws. Each state has its own constitution, statutes, bar rules, and traditions, and not surprisingly, the duties of attorneys general vary across the states. To simplify somewhat, we believe that there are three types of duties. One set of attorneys general has a duty to defend state law against state and federal challenges, while a second group has no duty to defend state law in such scenarios. A third cohort of attorneys general has a power (and in some cases a duty) to attack state statutes of dubious validity. They may (or must) proactively file suit to obtain judicial resolution of constitutional questions. Given that these duties vary across the states, politicians (including attorneys general) who blithely conclude that all state attorneys general must defend all state laws or, conversely, that all may refuse to defend whenever they believe a state law is unconstitutional evince a lamentable indifference to the power of states to craft an office that suits their particular needs. As the same-sex marriage debate reveals, categorical statements about whether state attorneys general must (or must not) defend bars on same-sex marriage are usually little more than self-serving sound bites from elected, politically ambitious attorneys general, intended for constituents focused on policy outcomes rather than legal questions. With Democrats and Republicans squarely divided on issues like same-sex marriage, gun control, and campaign finance, we predict that attorneys general will increasingly seek political advantage by refusing to defend (or insisting on the defense of) laws that divide the parties. We also foresee that failures to defend will be especially likely to occur in states where the attorney general is of a different political party than the governor, legislature, or the preceding attorney general.


The existence of transport costs among countries makes prices of tradables diverge. When the market structure is a differentiated oligopoly the prices of tradables increase as a country get larger and/or richer. In a framework of economies of scale-differentiation-monopolistic competition a less definite result can be found, since it all depends on the level of transport costs and the degree of openess. First we go through some theoretical aspects of these different approaches. Then, we provide empirical tests that may be able to discriminate among the two competing approaches. The results show that a relationship exists between size, percapita incomes and prices of tradables in countries separated by some transport cost. As a country is larger prices are lower, yet they become higher if percapita income is higher.


A direct link between a cancer-promoting protein kinase and the control of autophagy is presented. The protein kinase Akt is often activated in human cancers and is thought to promote tumor formation. One way in which it may do so is by inhibiting autophagy (a process by which the cell digests its own proteins or organelles, especially damaged ones). Wang et al. (see the Perspective by Koren and Kimchi) provide a direct molecular mechanism by which Akt regulates autophagy. Beclin, a component of the autophagy machinery, appears to be a direct target of phosphorylation by Akt. Such phosphorylation enhanced interaction of Beclin with intermediate filaments of the cytoskeleton and inhibited autophagy. Expression of a modified Beclin 1 molecule that could not be phosphorylated by Akt inhibited Akt-induced transformation of cells in culture and tumor formation in a mouse model. R. C. Wang, Y. Wei, Z. An, Z. Zou, G. Xiao, G. Bhagat, M. White, J. Reichelt, B. Levine, Akt-mediated regulation of autophagy and tumorigenesis through Beclin 1 phosphorylation. Science 338, 956–959 (2012). [Abstract] [Full Text] I. Koren, A. Kimchi, Promoting tumorigenesis by suppressing autophagy. Science 338, 889–890 (2012). [Abstract] [Full Text]


We have experimentally studied the polarization-dependent transmission properties of a nanoslit in a gold film as a function of its width. The slit exhibits strong birefringence and dichroism. We find, surprisingly, that the transmission of the polarization parallel to the slit only disappears when the slit is much narrower than half a wavelength, while the transmission of the perpendicular component is reduced by the excitation of surface plasmons. We exploit the slit's dichroism and birefringence to realize a quarter-wave retarder.


We investigate liquidity and trader behavior in the U.S. Treasury market during recent supply contractions. As in the precontraction period, dealers employ expandable order strategies to achieve greater-than-posted depth at the posted price and use expandable orders more often when expected information asymmetry is greater. Overall, however, dealers are less likely to discover greater-than-quoted depth during the supply contraction regimes. We find that, even after substantial losses in their market share of coupon Treasury trading, brokers reporting voice-brokered trading through GovPX provide an important protocol for depth discovery.We investigate liquidity and trader behavior in the U.S. Treasury market during recent supply contractions. As in the precontraction period, dealers employ expandable order strategies to achieve greater-than-posted depth at the posted price and use expandable orders more often when expected information asymmetry is greater. Overall, however, dealers are less likely to discover greater-than-quoted depth during the supply contraction regimes. We find that, even after substantial losses in their market share of coupon Treasury trading, brokers reporting voice-brokered trading through GovPX provide an important protocol for depth discovery.


Although cell phone use has grown dramatically, there is a gap in cell phone access between people with disabilities and the general public. The importance of cell phone use among people with intellectual disabilities and studies about use of cell phones by adults with intellectual disabilities was described. Our goal was to determine the extent and scope of cell phone use by 83 adults with intellectual disabilities, reasons for nonuse, and factors affecting use. Results suggest a gap in the use of cell phone technology between people with intellectual disabilities and the general population. When used, they are employed primarily for emergencies, storing telephone numbers, and day-to-day communication. Chief reasons for nonuse include cost, perception of not needing one, and lack of accessibility.


Social grants may play an important role in mitigating the impact of HIV/AIDS. Eligibility for these grants is driven in part by the increasing burden of chronic illness, the mounting orphan crisis and the impoverishment of households associated with the epidemic. This article investigates the role of social grants in mitigating the socio-economic impact of HIV/AIDS in South Africa, using data from a panel study on the household impact of the epidemic. Social grants reduce inequality and decrease the prevalence, depth and severity of poverty in affected households. However, these transfers also have disincentive effects on employment, while non-uptake is in some cases higher amongst the poorest. Copyright 2005 Economic Society of South Africa.


The natural design of the photosystems of plants and photosynthetic bacteria using chlorophylls (Chls) or bacteriochlorophylls (BChls) as photoreceptors are robust. The basic principles of the biological system of light-harvesting complex 2 (LH2) are studied with the use of natural and model sequences expressed in vivo in modified Rhodobacter (Rb) sphaeroides strains. Three aspects have been explored in the thesis: (1) BChl's macrocycle-protein interactions, (2) BChl's phytol-protein interactions underlying the structural and functional assembly of the pigment-protein complexes, and (3) LH2-lipid interactions and the role of these interactions in photosynthetic membrane morphogenesis.

BChls' macrocycle-protein interactions: Residues at the immediate BChl-B850/protein interface are found to have little effect on specifying the BChl-B850 array, and their light-harvesting activity in LH2. Nevertheless, these residues are important for the structural thermal stability. With the use of 'rescue' mutagenesis of the model BChl binding site, the hydrogen-bond between αSer -4 and the C131 keto carbonyl group of βBChl-B850 is shown to be a crucial motif for driving the assembly of model LH2 complex. Possibilities for residue modifications are limited in the β-subunits as compared to the α-subunits, which suggests that the two polypeptides have distinct roles in complex assembly. In the β-subunits, there are residues detected adjacent to the BChl-B850 site which are critical for the assembly of LH2.

BChls' phytol-protein interactions: Mutagenesis of residues closely interacting with the BChl-B850 phytol moiety result in the pronounced loss of BChl-B800 from LH2. Dephytylation of bound BChls within assembled LH2 to BChlides also resulted in the loss of BChl-B800 and destabilisation of LH2 structural assembly. Thus, the phytol chains were shown to be important for optimal pigment binding, especially for BChl-B800; which appears to be highly sensitive to the proper packing of the phytols. The pattern of phytol interactions with their surrounding environments are significantly different for α- and β-ligated (B)Chls. The phytols of β-ligated (B)Chls, as opposed to α-ligated (B)Chls, have ample and specific
interactions with residues of the binding helix which may contribute to the tertiary interactions of helices.

LH2-lipids interactions: Phospholipid determination of LH2 only expressing strains of Rb sphaeroides shows that the nonbilayer-forming phospholipid, phosphatidylethanolamine (PE) is present in elevated amounts in the intracytoplasmic membranes and in the immediate vicinity of the LH2 complex. In combination with βGlu -20 residue and the carotenoid headgroup at the N-terminus of the transmembrane β-helices is shown to influence the composition of lipids surrounding LH2. Specific local interactions between LH2 protein and lipids not only promote LH2 protein stability but appear to modulate the morphology of intracytoplasmic membranes. Based on these findings, the presence of LH2-lipid specificity is postulated. The approach of using model αβ-sequences with simplified pigment binding sites allows us to study the underlying factors involved in LH2 assembly and function. This gives rise to a better understanding of the interplay between BChl, apoproteins and membrane lipids in the assembly of a highly efficient light-harvesting complex in its native lipid-environment.


I investigate the optimal role of carbon sequestration for mitigation in the presence of a ceiling on atmospheric carbon concentration and consider aspects that have so far only been analyzed in the context of a damage function to measure the consequences of climate change for society. I assume extraction costs to be stock-dependent, replace the proportional decay description of the global carbon cycle by a two-box model, investigate the differences resulting from linear versus convex sequestration costs, and consider oceanic instead of geological carbon storage. Using a two-box model allows the non-renewable aspects of the global carbon cycle to be accounted for and implies that carbon emissions have to decline at the ceiling due to the ongoing saturation of the ocean with respect to anthropogenic carbon. Convex sequestration costs result in a continuous use of such a technology and allow the ceiling to be reached later than without sequestration, whereas linear sequestration costs result in a discontinuous use of such a technology and earlier reaching of the ceiling. Consequently, taking into the account the uncertainties in defining an appropriate ceiling, the policy recommendations with respect to carbon sequestration differ crucially according to the underlying assumptions of sequestration costs. Furthermore, the ocean might be a storage option for captured carbon, but even though its storage capacity is probably not scarce by itself, the ongoing saturation of the complete carbon cycle has to be taken into account.


Although data produced by governments are abundant, in developing countries data-informed public policy design and implementation is often more an aspiration than a reality. The Hands-on-Data (HoD) initiative is a successful practical experience that gathers several stakeholders (e.g., public servants, academia- and industry-based data scientists, and a multilateral financing institution) to prototype data science products addressing specific public sector needs in Latin American countries. The first edition of HoD took place in Argentina during the last quarter of 2018, and resulted in five prototypes that used artificial intelligence (AI) and machine learning (ML) to address narrowly defined public policy problems. Most importantly, HoD Argentina succeeded at organizing a seven-week task force consisting of public officials and data scientists, who extracted actionable knowledge from large datasets (e.g., administrative records) while ensuring anonymity and confidentiality of sensitive information. The policy domains analyzed in HoD Argentina were: work and labor, health, education, urban public transport, and accessibility in large cities. The short but intensive interaction between the public sector and data scientists sparked the interest of key stakeholders, and resulted in new co-creation initiatives that exceed the time frame of HoD. In this way, HoD acted as a stepping stone for new data collaborations. We discuss the relevant aspects behind the organization of this initiative, and we include summarized presentations of the five data science products that resulted from the first HoD. which impede many motivated and able public officers to learn and implement state-of-the-art data analysis tools.


Subtype heterogeneity for breast cancer risk factors has been suspected, potentially reflecting etiologic differences and implicating risk prediction. However, reports are conflicting regarding the presence of heterogeneity for many exposures. To examine subtype heterogeneity across known breast cancer risk factors, we conducted a case-control analysis of 2,632 breast cancers and 15,945 controls in Sweden. Molecular subtype was predicted from pathology record-derived IHC markers by a classifier trained on PAM50 subtyping. Multinomial logistic regression estimated separate ORs for each subtype by the exposures parity, age at first birth, breastfeeding, menarche, hormone replacement therapy use, somatotype at age 18, benign breast disease, mammographic density, polygenic risk score, family history of breast cancer, and BRCA mutations. We found clear subtype heterogeneity for genetic factors and breastfeeding. Polygenic risk score was associated with all subtypes except for the basal-like (Pheterogeneity < 0.0001). "Never breastfeeding" was associated with increased risk of basal-like subtype [OR 4.17; 95% confidence interval (CI) 1.89-9.21] compared with both nulliparity (reference) and breastfeeding. Breastfeeding was not associated with risk of HER2-overexpressing type, but protective for all other subtypes. The observed heterogeneity in risk of distinct breast cancer subtypes for germline variants supports heterogeneity in etiology and has implications for their use in risk prediction. The association between basal-like subtype and breastfeeding merits more research into potential causal mechanisms and confounders. Cancer Res; 77(13); 3708-17. ©2017 AACR.


The phase behavior of a symmetric styrene-isoprene (SI) diblock copolymer in a styrene-selective solvent, diethylphthalate, was investigated by in situ small-angle X-ray scattering on isotropic and shear-oriented solutions and by rheology and birefringence. A remarkable new feature in this phase diagram is the coexistence of both body-centered cubic (bcc) and hexagonally close-packed (hcp) sphere phases, in a region between close-packed spheres (cps) and hexagonally packed cylinders (hex) over the concentration range phi approximately 0.33-0.45. By focusing on the transitions among these various ordered phases during heating and cooling cycles, we observed a strong hysteresis: supercooled cylinders persisted upon cooling. The stability of these supercooled cylinders is quite dependent on concentration, and for phi > or = 0.40, the supercooled cylinders do not revert to spheres even after quiescent annealing for 1 month. The spontaneous formation of spheres due to the dissociation of cylinders is kinetically hindered in this case, and the system is apparently not amenable to any pretransitional fluctuations of cylinders prior to the cylinder-to-sphere transition. This contrasts with the case of cylinders transforming to spheres upon heating in the melt. The application of large amplitude shear to the supercooled cylinders is effective in restoring the equilibrium sphere phases.


In developmental biology, modeling and simulation play an important role in understanding cellular behavior. This article includes a review of the general models of development that are based on cellular interaction. Inspired by these models, we suggest a simple language, the Cell Programming Language (CPL), to write computer programs to describe this developmental behavior. These programs are an estimate of the minimal information needed to model realistically such developmental systems. Using these programs, it is possible to simulate and visualize cell behavior. We have employed CPL to model the following: aggregation in cellular slime mold in response to a chemotactic agent, cellular segregation and engulfment due to differential adhesion, and precartilage formation in vertebrate limbs. We believe CPL is a useful tool for developing, understanding, and checking biological models.


Using Musgrave's The Theory of Public Finance as a starting point, this paper reviews the scholarly developments in Public Sector Dynamics during the past three decades, placing emphasis not only on accomplishments but also areas in need of additional research. The review is organized into sections covering research on the public debt, its measurement and impact; the fiscal determinants of savings and the choice of tax base; the effects of taxation on investment and risk-taking; dynamic tax incidence; and dynamic inconsistency and public choice. Among the specific research topics considered are the Ricardian equivalence proposition, the incidence of the corporation income tax, the choice between income and consumption taxes, and political business cycles.


This paper describes various improvements in the areas of structure and visibility for Ada 2005. The most important improvement is perhaps the introduction of limited with clauses which permit types in two packages to refer to each other. A related addition to context clauses is the private with clause which just provides access from a private part. There are also important improvements to limited types which make them much more useful; these include initialization with aggregates and composition using a new form of return statement. This is one of a number of papers concerning Ada 2005 which are being published in the Ada User Journal. An earlier version of this paper appeared in the Ada User Journal, Vol. 26, Number 2, June 2005. Other papers in this series will be found in later issues of the Journal or elsewhere on this website.


Despite a vocabulary that consists of only three words Yes, No and And, Chil acts as a powerful speaker in conversation. He does this, embedding his limited lexicon within larger contextual configurations in which different kinds of meaning making processes including prosody, gesture, sequential organization, and operations on his talk by his interlocutors create a whole that goes beyond any of its constitutive parts. This paper explores the role played by prosody in this process. It focuses on how Chil is able to build varied action that is precisely fitted to its local environment by using different prosody over similar, and at times identical, lexical items, here pairs of No's. More generally it argues that analysis of human action should focus on the interdependent organization of diverse meaning making resources.