[Electrocardiographic picture in patients with congenital defect of atrial septum operated upon in adult age].
Electrocardiograms from 30 patients with congenital atrial septal defect operated upon in adult age were analyzed. One of frequent variants of QRS configuration in standard leads was SIqIII type. It was observed in 50% of patients. Most frequent (in 67% of patients) variants of QRS configuration in lead V1 were rSr' and rSR'. Signs of right ventricular hypertrophy were most often found in a group of patients with high pulmonary hypertension. Changes of terminal portion of ventricular complex such as negative or biphasic T waves were found in 29 of 30 patients (in 63% of cases these changes were localized in leads V1-V3).


An alternative method for analysis of food taints using stir bar sorptive extraction.
The determination of taints in food products currently can involve the use of several sample extraction techniques, including direct headspace (DHS), steam distillation extraction (SDE) and more recently solid phase microextraction (SPME). Each of these techniques has disadvantages, such as the use of large volumes of solvents (SDE), or limitations in sensitivity (DHS), or have only been applied to date for determination of individual or specific groups of tainting compounds (SPME). The use of stir bar sorptive extraction (SBSE) has been evaluated as a quantitative screening method for unknown tainting compounds in foods. A range of commonly investigated problem compounds, with a range of physical and chemical properties, were examined. The method was optimised to give the best response for the majority of compounds and the performance was evaluated by examining the accuracy, precision, linearity, limits of detection and quantitation and uncertainties for each analyte. For most compounds SBSE gave the lowest limits of detection compared to steam distillation extraction or direct headspace analysis and in general was better than these established techniques. However, for methyl methacrylate and hexanal no response was observed following stir bar extraction under the optimised conditions. The assays were carried out using a single quadrupole GC-MS in scan mode. A comparison of acquisition modes and instrumentation was performed using standards to illustrate the increase in sensitivity possible using more targeted ion monitoring or a more sensitive high resolution mass spectrometer. This comparison illustrated the usefulness of this approach as an alternative to specialised glassware or expensive instrumentation. SBSE in particular offers a 'greener' extraction method by a large reduction in the use of organic solvents and also minimises the potential for contamination from external laboratory sources, which is of particular concern for taint analysis.


Buccal Myomucosal Flap for Reconstruction of Red Lip Defects Close to Mouth Angle.
Owing to the special esthetic and functional role in the face, red lip reconstruction presents a challenge to plastic surgeons. Various reconstructive techniques can be employed to fix the red lip defects close to the mouth angle. The purpose of this study was to demonstrate that the buccal myomucosal flap could be an available option to repair red lip defects with preservation of its esthetics and function. A single-center, retrospective study of 7 patients with red lip defects who were treated with buccal myomucosal flap was conducted between June 2017 and March 2022. All patients were followed up for at least 6 months including questionnaires and photography. Of these 7 patients, 2 were women, and 5 were men. The average age was 14.3 years (range, 1-32 y). All the buccal myomucosal flaps survived well. All the donor sites were closed directly without complications. The average follow-up time was 33.4 months (range, 6-57 mo). All patients were satisfied with the aesthetic and functional results. The buccal myomucosal flap is versatile and reliable, with the advantages of rich vascularity, flexible design, and easy access. This study was presented to highlight that the flap could be a good candidate to treat red lip defects close to the mouth angle.


Reexploration after treatment for uterine sarcoma.
Eleven patients treated with combination chemotherapy and radiotherapy for uterine sarcoma were reexplored after an interval of therapy. In none of the eight patients who were free of persistent sarcoma at the time of reexploration, did tumor recur during the period of follow-up. Two patients had all macroscopically visible residual sarcoma resected at reexploration and remain free of disease. The second-look laparotomy after a period of therapy may provide valuable prognostic information for planning therapy for patients who have metastatic or locally recurrent sarcoma.


Fixed-final-time optimal control of nonlinear systems with terminal constraints.
A model-based reinforcement learning algorithm is developed in this paper for fixed-final-time optimal control of nonlinear systems with soft and hard terminal constraints. Convergence of the algorithm, for linear in the weights neural networks, is proved through a novel idea by showing that the training algorithm is a contraction mapping. Once trained, the developed neurocontroller is capable of solving this class of optimal control problems for different initial conditions, different final times, and different terminal constraint surfaces providing some mild conditions hold. Three examples are provided and the numerical results demonstrate the versatility and the potential of the developed technique.


[Sperm chromatin integrity disturbances in infertile men].
A survey of knowledge and detection methods of sperm chromatin integrity disturbances. Review of literature. Veterinary Research Institute, Brno. A survey of experience with detection methods for human sperm chromatin integrity disturbances. Disturbed sperm chromatin integrity manifested as an increase in DNA strand breaks within the spermatozoa and deficient chromosome condensation in the sperm nucleus is one of the causes of male infertility. Various approaches to the detection of such defects have been described. The methods differ above all in the time and expenses required, and robustness. The review brings a survey of the most commonly used procedures, and compares advantages and disadvantages of respective methods.


Production and application of monoclonal antibodies against ORF66 of cyprinid herpesvirus 2.
Cyprinid herpesvirus 2(CyHV-2)is the main pathogen causing haematopoietic necrosis disease of goldfish (Carassius auratus auratus) and gibel carp (Carassius auratus gibelio), which has caused huge economic losses to aquaculture industry of goldfish and gibel carp around the world. Currently, various detection methods based on nucleic acids have been established for the detection of CyHV-2. However, there is still a lack of rapid and effective immunological detection technology. In this study, anti-CyHV-2 ORF66 monoclonal antibodies (MAbs) were prepared to use the recombinant ORF66 protein as the antigen. Firstly, the open reading frame of CyHV-2 ORF66 was cloned into the pET-28a vector and expressed in Escherichia coli. Three MAbs (2F11, 2G8, and 3D6) against recombinant ORF66 protein were developed by immunization of Balb/C mice. Among them, MAb-2F11 belonged to the IgG2b isotype, 2G8 and 3D6 belonged to the IgG1 isotype. Western blotting analysis was performed to assess the ability of the MAbs to bind to the ORF66 recombinant protein and CyHV-2 nucleocapsid protein ORF66. In addition, the MAb-2F11 was used to detect the virus particles that infected in cell line and tissues of gibel carp virus infection by immunological methods. These results indicated that the anti-CyHV-2 ORF66 MAb-2F11 prepared in this study could not only detect the presence of the virus but also provide a research tool for further studying the role of ORF66 in the process of CyHV-2 infection.


Prediction of translation initiation sites in human mRNA sequences with AUG start codon in weak Kozak context: A neural network approach.
Translation of eukaryotic mRNAs is often regulated by nucleotides around the start codon. A purine at position -3 and a guanine at position +4 contribute significantly to enhance the translation efficiency. Algorithms to predict the translation initiation site often fail to predict the start site if the sequence context is not present. We have developed a neural network method to predict the initiation site of mRNA sequences that lack the preferred nucleotides at the positions -3 and +4 surrounding the translation initiation site. Neural networks of various architectures comprising different number of hidden layers were designed and tested for various sizes of windows of nucleotides surrounding translation initiation sites. We found that the neural network with two hidden layers showed a sensitivity of 83% and specificity of 73% indicating a vastly improved performance in successfully predicting the translation initiation site of mRNA sequences with weak Kozak context. WeakAUG server is freely available at http://bioinfo.iitk.ac.in/AUGPred/.


Interpersonal and communication skills development in general nursing preceptorship education and training programmes: A scoping review.
The aim of this systematic scoping review is to examine and synthesise the available literature on developing interpersonal and communication skills in general nursing preceptorship education programmes. Highly developed interpersonal and communication skills are an essential component of nursing preceptorship. Preceptors are integral in facilitating, guiding, and developing positive interpersonal relationships between the nursing student (the preceptee), the qualified nurse (the preceptor), and patients. They also have a responsibility to foster and develop preceptees' interpersonal and communication skills and assess and deem preceptees as competent in such skills. Furthermore, preceptors require effective interpersonal and communication skills to carry out key responsibilities of their role, including creating a safe clinical learning environment, teaching, and providing effective feedback. A scoping review was conducted using the methodological framework of Arksey and O'Malley. The PRISMA Extension for Scoping Reviews guided the reporting. Five electronic databases were searched for relevant articles in consultation with a librarian, supplemented by hand-searching and internet searches for grey literature. A total of 19,431 potentially relevant articles published between Jan 2000 and August 2021 were retrieved from the initial search, and an additional six articles were obtained from the supplemental search. A total of 146 articles were independently reviewed by two researchers, resulting in 24 articles eligible for inclusion in the review. Several themes were identified in the literature that influenced the development of interpersonal and communication skills in preceptorship education and training programmes, including design and development of programmes, key learning outcomes, pedagogical approaches of preceptorship education and training programmes and interpersonal & communication skills development in preceptorship education and training programmes. Conclusions This review highlighted that research on developing interpersonal and communication skills amongst preceptors is mainly absent from the literature. Further research to address these knowledge gaps is warranted. The results from this review can be used to inform future curriculum design and development of nursing preceptorship education and training programmes.


Postcontrast Acute Kidney Injury After Computed Tomography Pulmonary Angiography for Acute Pulmonary Embolism.
Despite the widespread use of computed tomography pulmonary angiography with contrast media for the diagnosis of acute pulmonary embolism, high-quality evidence on risk factors for postcontrast acute kidney injury related to its use is lacking. The present study aimed to investigate whether the level of estimated glomerular filtration rate observed in the emergency department (ED) is significantly associated with the occurrence of postcontrast acute kidney injury in patients undergoing computed tomography pulmonary angiography. We performed a retrospective observational study using data automatically collected by a clinical data retrieval system from 1300 patients who underwent computed tomography pulmonary angiography for suspected acute pulmonary embolism in the ED. A total of 632 patients were selected for the study after exclusion. Univariate analyses were performed to identify significant risk factors for postcontrast acute kidney injury (the primary outcome). Multivariate logistic regression analysis was used to confirm the effect of estimated glomerular filtration rate in the ED on the occurrence of postcontrast acute kidney injury after adjustment for confounding variables. The total incidence rate of postcontrast acute kidney injury was 6.49% (41/632 patients). No statistically significant association between estimated glomerular filtration rate and the risk of postcontrast acute kidney injury was observed. Our study findings could serve as useful reference for physicians who are concerned about performing computed tomography pulmonary angiography for fear of renal function deterioration.


Current status and future expectations for multisite pacing in heart failure.
The number of patients with congestive heart failure (CHF) has achieved astonishing proportions. It is a debilitating and usually lethal condition, aside from being responsible for an enormous proportion of health care expenditures. Advances in medical therapy have not been sufficient to significantly improve prognosis, and heart transplantation can only benefit a minority of patients. Biventricular pacing has emerged as a promising form of therapy for patients with severe, medical refractory CHF with ventricular conduction defects. However, there are many technical issues to be solved, and better methods of selecting patients who respond favorably to this form of therapy are yet to be developed. This article reviews the rationale, delivery modes, and available data supporting multisite cardiac pacing as an alternative form of therapy for the failing heart.


Crustacean larval factor shares structural characteristics with the insect-specific follicle cell protein.
Literature on the cuticle formation in larval stages of the diverse group of decapod crustaceans is lacking, as opposed to a wealth of knowledge in several insect groups. Here we provide the first glimpse of the cuticular organisation in larvae of the eastern spiny lobster Sagmariasus verreauxi. A bioinformatic approach applied to S. verreauxi transcriptome through metamorphosis identified for the first time a small secreted protein with multiple isoforms that is highly expressed in crustacean larvae. This protein, named crustacean larval factor (Clf) shares structural characteristics with insect follicle cell protein 3 (FCP3), an insect-specific, rapidly evolving protein, with spatial-temporal regulated expression that is restricted to follicular cells during the production of the vitellin coat. Furthermore, we identified the FCP3 domain in additional structural proteins in multiple arthropod groups. Recombinant Clf inhibited in vitro calcium carbonate crystalline precipitation, in keeping with the finding that the spiny lobster larval cuticle is mainly composed of amorphous calcium carbonate. In addition, the recombinant Clf was shown to bind chitosan. Taken together, this research identifies two novel structural domains with lineage-specific expansion across arthropods. In crustaceans, Clf is found predominantly in larvae and the spatial-temporal regulated FCP3 factor occurs as a domain identified in multiple structural proteins across arthropods. Given the shared ten cysteines backbone between the Clf and FCP domains, a shared evolution is suggested and should be further explored.


Volumetric and dosimetric evaluation of radiation treatment plans: radiation conformity index.
The use of conformal radiation therapy has grown substantially during the last years since three-dimensional (3D) treatment planning systems with beams-eye-view planning has become commercially available. We studied the degree of conformity reached in clinical routines for some common diagnoses treated at our department by calculating a radiation conformity index (RCI). The radiation conformity index, determined as the ratio between the target volume (PTV) and the irradiated volume, has been evaluated for 57 patients treated with 3D treatment plans. The RCI was found to vary from 0.3 to 0.6 (average 0.4), a surprisingly low figure. The higher RCI is typical for pelvic treatments (e.g., prostate) and stereotactic treatments. The lower RCI is found for extended tumors, such as mammary carcinomas where the adjacent nodes are included. The latter is also valid for most lung cancer patients studied. The RCI gives a consistent method for quantifying the degree of conformity based on isodose surfaces and volumes. Care during interpretation of RCI must always be taken, since small changes in the minimum dose can dramatically change the treated volume.


Antibiotic prophylaxis for urinary tract infection after midurethral sling: a randomized controlled trial.
Because of the reported high percentage of bladder infections after placement of a midurethral sling (8.9%-34%), this study was undertaken to determine if a 3-day postoperative course of a urinary antibiotic would significantly lower the incidence of urinary tract infection (UTI) among treated women. Women who were planning outpatient vaginal surgery for stress incontinence were enrolled, after informed consent, and randomized to receive placebo or nitrofurantoin (100 mg 2 times a day) for 3 days after surgery. Research subjects and investigators were blinded to the randomization. Women were observed for signs and symptoms of UTI until 6 weeks after surgery. Sample size was calculated to have 80% power to detect a 15% difference in the primary outcome (α = 0.05). The primary outcome was analyzed using a χ analysis. Data were analyzed from 149 subjects (placebo, n = 75; drug, n = 74). Overall, 37 women (24.8%) were diagnosed with a UTI during the study period. The incidence of postoperative UTI was significantly lower in the treatment arm (17.6%) then in the placebo arm (32%; P = 0.04). A short (3-day) course of nitrofurantoin after outpatient sling surgery significantly reduces the incidence of postoperative bladder infection in the first 6 weeks after sling surgery.


An investigation of aldehyde fuchsin staining of unoxidized insulin.
Aldehyde fuchsin is a standard stain for the secretion granules of pancreatic B cells. The participation of either insulin or proinsulin in aldehyde fuchsin staining is in dispute. There is some evidence that permanganate oxidized insulin is stained by aldehyde fuchsin. Aldehyde fuchsin staining of unoxidized insulin has not been investigated adequately despite excellent staining results with tissue sections. Unoxidized insulin and proinsulin suspended by electrophoresis in polyacrylamide gels were fixed with Bouin's fluid and placed in aldehyde fuchsin for one hour. Because the unoxidized proteins were not stained by aldehyde fuchsin, it was concluded that neither insulin or proinsulin are responsible for the intense aldehyde fuchsin staining of unoxidized pancreatic B cell granules in tissue sections. A series of controlled experiments was undertaken to test the effects of fixatives, oxidation and destaining procedures on aldehyde fuchsin staining of insulin, proinsulin and other proteins immobilized in polyacrylamide gels. It was demonstrated that only oxidized proteins were stained by aldehyde fuchsin and that cystine content of the proteins had no apparent relation to aldehyde fuchsin staining. It was concluded that neither insulin nor proinsulin is likely to be responsible for the intense aldehyde fuchsin staining of unoxidized pancreatic B cell granules in tissue sections.


Coronary artery bypass graft surgery after acute myocardial infarction caused by thrombosis of coronary aneurysm.
The morphology of coronary aneurysm sets a predisposing factor to thrombus formation. However, the blood stasis caused by the change in flow may not be enough to cause thromboembolic events.


A demonstration of vascular proliferation in response to arteriovenous reversal in the ischemic canine hind limb.
Patients with diffuse peripheral vascular disease, resulting in inadequate arteriographic runoff, and/or multiple failed bypasses almost inevitably face amputation. We have previously used an arteriovenous reversal (AVR) procedure at the popliteal level to salvage severely ischemic canine hind limbs. This procedure is followed by the development of an intense network of new vessels in the proximity of the arteriovenous anastomosis. The present study was designed to define the nature of these vessels and their role in providing nutrient perfusion to the limb. Using an established model to create severe limb ischemia. AVR was performed in 24 dogs. Tissue perfusion and neovascularization were assessed by angiography, transcutaneous oximetry (TcpO2), tritiated thymidine uptake combined with autoradiography, and histologic capillary density measurements. Angiography revealed the intense vascular network in the region of the anastomosis by postoperative Day 22 in all dogs. TcpO2 on Day 1 was indicative of severe ischemia in the operated calf compared to controls (12 vs 80 mm Hg, P less than 0.01) but returned to normal by Day 22 (81 vs 76 mm Hg). A progressive, significant increase in tritiated thymidine uptake was seen by Day 22 (0.36 vs 0.27 microCi/g, P = 0.005); on autoradiography 95% of these labeled nuclei in the operated limbs were in endothelial cells compared to only 5% in the control limbs. Finally, capillary density studies of the calf muscles showed a substantial increase in the operated limbs (mean, 1011 vs 605 cap/mm2, P = 0.0004). These studies suggest that the dramatic increase in vascularity observed angiographically following AVR in a severely ischemic limb consists primarily of a rapidly expanding capillary network.(ABSTRACT TRUNCATED AT 250 WORDS)


Food security among young adults with disabilities in the United States: Findings from the National Health Interview Survey.
Prior research has suggested that young adults with disabilities face economic, health and social disadvantage. Food security, an area of disadvantage that can influence overall health, has not been fully explored for this population. To examine levels of food security between young adults with and without disabilities, controlling for individual characteristics. Logistic regression analysis of a nationally representative sample of young adults (age 18-25) (n = 32,795) with and without disabilities, using pooled data form the 2011-2013 National Health Interview Survey. Young adults with disabilities have significantly higher odds (OR: 2.58, p < 0.001) of living in a household that is food insecure than young adults without disabilities, even when controlling for individual characteristics. Odds of living in a household that is food insecure are particularly high (OR: 5.35, p < 0.001) among young adults with high levels of psychological distress, controlling for other factors. Young adults with disabilities have increased odds of living in a household that is food insecure. This study has important policy and community program implications.


Combined three-dimensional gait and plantar pressure analyses detecting significant functional deficits in children with juvenile idiopathic arthritis.
Children suffering from juvenile idiopathic arthritis (JIA), a heterogeneous group of chronic inflammatory joint diseases, adapt to individual gait patterns to avoid loading of inflamed, swollen and painful joints. As the interpretability of previous studies is limited, this study aims to assess the functional capacity, loads and plantar pressure distribution in the gait of a homogeneous JIA group. Does a symmetrical lower limb joint involvement influence the gait dynamics in JIA patients, and how are the results of three-dimensional gait analysis (3DGA) and pedobarography related? Fifty JIA patients with symmetrical hip, knee and ankle joint arthritis and 27 healthy controls performed 3DGA and pedobarography at self-selected walking speeds. Kinematics and kinetics of lower limb joints were retrospectively compared in range of motion and in time-normalized waveforms. Plantar load was evaluated by measuring peak pressure, pressure-time integral and maximum force of the whole foot and ten selected foot regions. 1D-SPM analysis, parametric and non-parametric statistical significance tests and correlation coefficients were used for statistical analysis. JIA patients had a significantly slower walking speed with an anteriorly tilted pelvis and a reduced extension motion of all joints of the lower limb. The horizontal ground reaction forces and generated hip and ankle power during propulsion phase were small. Patients experienced reduced loading at toe regions, which correlated with limited ankle plantarflexion motion in the push-off phase. The total peak pressure was significantly increased and loads at lateral midfoot and metatarsal regions were higher in patients. Symmetrical lower limb arthritis is linked to crouch-like gait and restricted gait dynamics with increased total peak pressure. The results confirm earlier results of 3DGA and provide new insights regarding waveform analysis and plantar loading in JIA patients. The used methods help to design individualized functional treatment of JIA patients.


A new chevron electromagnetic acoustic transducer design for generating shear horizontal guided wave.
Guided wave electromagnetic acoustic transducers (EMATs) have a significant role in non-destructive testing and structural health monitoring. It can generate horizontally polarized shear waves propagating axially or circumferentially to characterize the shape and orientation of pipeline defects. This work proposes a new Chevron pattern-like coil structure design with a periodic permanent magnet (PPM) EMAT configuration. This specific design of the EMAT coil can generate a bi-directional shear horizontal wave (SH-wave) and reduce the side lobes. An optimal Chevron angle of coil wires assists in generating orthogonal propagatingwaveforms. These phenomena lead to the constructive interference of propagating waves and develop a resulting wave along the horizontal direction. A 3D FEM modeling and simulation have been carried out and validated with experimental results. The proposed EMAT results are compared with the conventional racetrack PPM EMAT model, which shows a significant improvement over conventional EMATs. A prototype of this proposed EMAT has been developed. It can be used to inspect surface defects in applications such as fuel transportation pipelines.


Colonization of the gastric juice by Candida spp. promotes surgical site infection after hepatectomy.
Candida spp. cause opportunistic infections in conditions of immunodeficiency. Here, we investigated the relationship between colonization of the gastric juice by Candida spp. and surgical site infection (SSI) in hepatectomy. Consecutive hepatectomy cases between November 2019 and April 2021 were enrolled. Gastric juice samples (collected intraoperatively through a nasogastric tube) were cultured. We compared factors related to patient background, blood test findings, surgical findings, and postoperative complications between the Candida + group (positive for colonization of the gastric juice by Candida spp.) and the Candida - group (negative). In addition, we identified the factors that contribute to SSI. There were 29 and 71 patients in the Candida + and Candida - groups, respectively. The Candida + group was significantly older (average age: Candida + 74 years vs. Candida - 69 years; p = 0.02) and contained more patients negative for the hepatitis B and C virus (Candida + 93% vs. Candida - 69%; p = 0.02). SSI was significantly more common in the Candida + group (Candida + 31% vs. Candida - 9%; p = 0.01). Postoperative bile leakage and colonization of the gastric juice by Candida spp. were independent predictors of SSI. Colonization of the gastric juice by Candida spp. is a risk factor for SSI after hepatectomy.


Threshold and resilience management of coupled urbanization and water environmental system in the rapidly changing coastal region.
The concept of thresholds shows important implications for environmental and resource management. Here we derived potential landscape thresholds which indicated abrupt changes in water quality or the dividing points between exceeding and failing to meet national surface water quality standards for a rapidly urbanizing city on the Eastern Coast in China. The analysis of landscape thresholds was based on regression models linking each of the seven water quality variables to each of the six landscape metrics for this coupled land-water system. We found substantial and accelerating urban sprawl at the suburban areas between 2000 and 2008, and detected significant nonlinear relations between water quality and landscape pattern. This research demonstrated that a simple modeling technique could provide insights on environmental thresholds to support more-informed decision making in land use, water environmental and resilience management.


A numerical study on nonlinear dynamics of oscillatory time-depended viscoelastic flow between infinite parallel plates: utilization of symmetric and antisymmetric Chandrasekhar functions.
The one-dimensional viscoelastic fluid flow between two infinite parallel plates with oscillatory inlet condition is examined using the Johnson-Segalman model. The symmetric and antisymmetric Chandrasekhar functions in space are utilized to represent the velocity and stress fields. The non-dimensional form of the conservation laws in addition to the constitutive equations are solved numerically based on the Galerkin projection method. Two critical Weissenberg numbers (We) for various Reynolds numbers (Re) and viscosity ratios (ε) are obtained to determine the stable range of nonlinear system behavior. Moreover, for the unsteady case, the effects of Re, viscosity ratio of solvent to solution as well as We are investigated. According to the obtained results, increasing of oscillations frequency in subcritical zone, the same as low frequency case, has almost no effect on the velocity and its gradient. Nevertheless, the normal stress amplitude of oscillations is reduced. The Re number determines the number of oscillations and the needed time prior to the steady condition. For lower Re, due to higher effect of viscosity, the initial fluctuations are intensely occurred in a short time period in contrary to the high Re case.


Dynamic contact angle measurements on lubricant infused surfaces.
Even though lubricant-infused surfaces (LISs) are known to affect the mobility of working fluid depending on the infused lubricant, previous studies have not yet quantified their slippery property. This study proposes the slippery nature of the LIS can be assessed by dynamic contact angles of the working fluid on the LIS and its scaling model. We measured the apparent dynamic advancing and receding contact angles on a LIS using a modified Wilhelmy plate technique for the first time. Lubricant having different viscosities was infused into the sanded polytetrafluoroethylene surface to fabricate the LIS. The surface was immersed into or withdrawn from an aqueous glycerol-water solution by varying the capillary number and the lubricant viscosity. The dynamic contact angles on LIS was found to be sensitive to changes in both the lubricant viscosity and the capillary number. The cube of the dynamic contact angles on the LIS was proportional to θD3~Ca1, which follows a conventional hydrodynamic theory. In addition, the decreasing lubricant viscosity shifted the cube of the dynamic contact angles to high capillary numbers. Our dynamic contact angle data coincided with the prediction from a scaling law derived in this study.


NDRG2 is involved in the oncogenic properties of renal cell carcinoma and its loss is a novel independent poor prognostic factor after nephrectomy.
Although NDRG2 is a candidate tumor suppressor, its exact role in renal cell carcinoma (RCC) is not fully understood. We investigated the functional role of NDRG2 and its clinical relevance in RCC tumorigenesis. NDRG2 expression and its clinical implications in clear cell RCC were evaluated. Biological function was assessed by a proliferation assay, anchorage-independent growth assay, and wound healing and transwell migration assays in RCC cell lines overexpressing NDRG2 coupled with an investigation of the effects of NDRG2 expression on the epithelial-mesenchymal transition (EMT). NDRG2 was differentially expressed in patients with RCC. A loss of NDRG2 was significantly associated with a higher proportion of tumors >10 cm and a high nuclear grade. Furthermore, multivariate analyses indicated that a loss of NDRG2 was an independent poor prognostic factor for patient survival (recurrence-free survival, hazard ratio 7.901; disease-specific survival, hazard ratio 15.395; overall survival, hazard ratio 11.339; P < 0.001 for all parameters). NDRG2 expression inhibited the anchorage-independent growth and migration of RCC cells. NDRG2 expression also modulated the expression of EMT-related genes such as Snail, Slug, and SIP1, and it decreased EMT signaling in RCC cells. Finally, NDRG2 recovered E-cadherin expression in E-cadherin-negative RCC cells. These results indicate that a lack of NDRG2 is associated with oncogenic properties through the loss of its role as a tumor suppressor, and that NDRG2 is an independent poor prognostic factor predicting survival in clear cell RCC, suggesting that it can serve as a novel prognostic biomarker.


Neurologic manifestations of AIDS: a review of fifty cases in Santos, São Paulo, Brazil.
To review the neurologic manifestations of AIDS in patients who were admitted to Hospital Guilherme Alvaro (HGA) due to any clinical manifestation of the disease. Case series. All HIV+ patients admitted to the Faculty Hospital (HGA) between July 96 and April 97 were included in this review. From the 117 HIV+ patients admitted to hospitalization due to AIDS-related symptoms, 50 (42.7%) presented neurologic manifestations. The most prevalent of these was neurotoxoplasmosis (68%), but a variety of other neurologic diseases were observed. Only 36% of these 50 patients had neurological signs and symptoms as the main complaint for admission, 12% of the patients had at least complained of some neurologic dysfunction at the time of admission and 10% had no neurologic complaints at all. The remaining 42% (21 patients) only complained of neurologic manifestations of AIDS when specifically asked. The prevalence of neurologic manifestations of AIDS is very high in patients admitted to hospital. Even in the absence of neurologic-related complaints, these patients have to be carefully questioned and examined in the search for an underlying neurologic complication which may present high morbidity and mortality.


An optimized calibration method for surface measurements with MOSFETs in shaped-beam radiosurgery.
Nowadays MOSFET dosimeters are widely used for dose verification in radiotherapy procedures. Although their sensitive area satisfies size requirements for small field dosimetry, their use in radiosurgery has rarely been reported. The aim of this study is to propose and optimize a calibration method to perform surface measurements in 6 MV shaped-beam radiosurgery for field sizes down to 18 × 18 mm(2). The effect of different parameters such as recovery time between 2 readings, batch uniformity and build-up cap attenuation was studied. Batch uniformity was found to be within 2% and isocenter dose attenuation due to the build-up cap over the MOSFET was near 2% irrespective of field size. Two sets of sensitivity coefficients (SC) were determined for TN-502RD MOSFET dosimeters using experimental and calculated calibration; the latter being developed using an inverse square law model. Validation measurements were performed on a realistic head phantom in irregular fields. MOSFET dose values obtained by applying either measured or calculated SC were compared. For calibration, optimal results were obtained for an inter-measurement time lapse of 5 min. We also found that fitting the SC values with the inverse square law reduced the number of measurements required for calibration. The study demonstrated that combining inverse square law and Sterling-Worthley formula resulted in an underestimation of up to 4% of the dose measured by MOSFETs for complex beam geometries. With the inverse square law, it is possible to reduce the number of measurements required for calibration for multiple field-SSD combinations. Our results suggested that MOSFETs are suitable sensors for dosimetry when used at the surface in shaped-beam radiosurgery down to 18 × 18 mm(2).


Baculovirus-based Vaccine Displaying Respiratory Syncytial Virus Glycoprotein Induces Protective Immunity against RSV Infection without Vaccine-Enhanced Disease.
Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract diseases in infancy and early childhood. Despite its importance as a pathogen, there is no licensed vaccine against RSV yet. The attachment glycoprotein (G) of RSV is a potentially important target for protective antiviral immune responses. Recombinant baculovirus has been recently emerged as a new vaccine vector, since it has intrinsic immunostimulatory properties and good bio-safety profile. We have constructed a recombinant baculovirus-based RSV vaccine, Bac-RSV/G, displaying G glycoprotein, and evaluated immunogenicity and protective efficacy by intranasal immunization of BALB/c mice with Bac-RSV/G. Bac-RSV/G efficiently provides protective immunity against RSV challenge. Strong serum IgG and mucosal IgA responses were induced by intranasal immunization with Bac-RSV/G. In addition to humoral immunity, G-specific Th17- as well as Th1-type T-cell responses were detected in the lungs of Bac-RSV/G-immune mice upon RSV challenge. Neither lung eosinophilia nor vaccine-induced weight loss was observed upon Bac-RSV/G immunization and subsequent RSV infection. Our data demonstrate that intranasal administration of baculovirus-based Bac-RSV/G vaccine is efficient for the induction of protection against RSV and represents a promising prophylactic vaccination regimen.


Pharmacognostic approach to evaluate the micromorphological, phytochemical and biological features of Citrus lumia seeds.
This study evaluated the micro-morphology as well as the chemical and biological features of Citrus lumia seeds. The cream-colored pyriform seed showed a woody coat covered by a thick layer of mucilage and an embryo with two large cotyledons rich in oil bodies. Hydroxycinnamic acid glycosides and flavonoids are the most abundant compounds in methanol and ethyl acetate extracts (ME and EAE), respectively. Conversely, fatty acids and α-tocopherol represent the main bioactive compounds in the hexane extract (HE). ME showed the most promising antioxidant and anti-inflammatory activities already in cell-free assays. These results were confirmed by experiments carried out on human primary cells. Indeed, ME showed the best inhibitory activity against heat-induced haemolysis and ROS formation in erythrocytes. Moreover, the same order of potency (ME > EAE > HE) was observed also on peripheral blood mononuclear cells, in which the seed extracts were able to decrease TNF-α and IL-6 release after LPS-induced inflammation.


Incremental Effect of Aging on Obesity-Related Incident Chronic Kidney Disease in the Korean General Population.
Obesity may increase the risk of kidney function decline. However, few studies have addressed how age modifies obesity-associated risk of chronic kidney disease (CKD) in the Asian general population. A community-based prospective cohort study. 6538 Korean general population with normal kidney function from the Korean Genome and Epidemiology Study Database. Participants were stratified according to age (40-49, 50-59, and 60-69 years old) and body mass index [≥18.5-<23 (normal-weight), ≥23-<27.5 (overweight), and ≥27.5 (obese)]. We conducted Kaplan-Meier and multivariable-adjusted Cox regression analyses to investigate the association of aging and obesity with incident CKD. During the 12-year follow-up, an overall incidence rate of CKD was 6.1 cases per 1000 person-years. Obese, but not overweight, people had an increased risk of incident CKD compared with normal-weight people in multivariable models adjusted for metabolic factors. When analyzed by 10-year increments, this association was significant only in 60-69-year-old individuals. Kaplan-Meier analysis showed that the incidence of CKD associated with overweight or obesity showed an accentuated increase with age. With reference to normal-weight individuals aged 40-49 years, the adjusted hazard ratio of CKD increased with age regardless of body mass index, and the positive association between obesity and incident CKD was more prominent with increasing age. Obesity-associated risk of incident CKD was accentuated in older people, and this association was independent of metabolic abnormalities.


Cervical cord neurons labeled by horseradish peroxidase application to the sciatic nerve in the rat.
After horseradish peroxidase (HRP) application to the proximal cut end of the sciatic nerve in rats aged 3-10 days, HRP-labeled neuronal cell bodies were found ipsilaterally in the ventrolateral region of the ventral horn in the cervical enlargements of the spinal cord. Such labeled neurons were occasionally seen in rats aged 15 days, but not seen at all in rats aged 60-90 days. The labeling was presumably the result of a retrograde transneuronal axonal transport of HRP applied to the sciatic nerve.


Strong seasonality of Bonamia sp. infection and induced Crassostrea ariakensis mortality in Bogue and Masonboro Sounds, North Carolina, USA.
Asian oyster Crassostrea ariakensis is being considered for introduction to Atlantic coastal waters of the USA. Successful aquaculture of this species will depend partly on mitigating impacts by Bonamia sp., a parasite that has caused high C. ariakensis mortality south of Virginia. To better understand the biology of this parasite and identify strategies for management, we evaluated its seasonal pattern of infection in C. ariakensis at two North Carolina, USA, locations in 2005. Small (<50 mm) triploid C. ariakensis were deployed to upwellers on Bogue Sound in late spring (May), summer (July), early fall (September), late fall (November), and early winter (December) 2005; and two field sites on Masonboro Sound in September 2005. Oyster growth and mortality were evaluated biweekly at Bogue Sound, and weekly at Masonboro, with Bonamia sp. prevalence evaluated using parasite-specific PCR. We used histology to confirm infections in PCR-positive oysters. Bonamia sp. appeared in the late spring Bogue Sound deployment when temperatures approached 25 degrees C, six weeks post-deployment. Summer- and early fall-deployed oysters displayed Bonamia sp. infections after 3-4 weeks. Bonamia sp. prevalences were 75% in Bogue Sound, and 60% in Masonboro. While oyster mortality reached 100% in late spring and summer deployments, early fall deployments showed reduced (17-82%) mortality. Late fall and early winter deployments, made at temperatures <20 degrees C, developed no Bonamia sp. infections at all. Seasonal Bonamia sp. cycling, therefore, is influenced greatly by temperature. Avoiding peak seasonal Bonamia sp. activity will be essential for culturing C. ariakensis in Bonamia sp.-enzootic waters.


Molecular cloning of a putative homolog of proline/arginine-rich antibacterial peptides from porcine bone marrow.
Screening of a porcine bone marrow cDNA library with a PCR-derived probe from rabbit LPS-binding protein CAP18 led to the discovery of two closely related clones. The longer, full-length cDNA clone encodes a 228 amino acid residue protein similar to the family of antibacterial/LPS-binding cationic peptides. In contrast to other hitherto discovered precursors of Pro/Arg-rich peptides from this family, they have a novel, unique structure of the C-terminal region of 100 amino acid residues with a repeating sequence of ten residues (FPPPNXPGPR, where X = V or F). These precursors could represent a part of the antibacterial peptide repertoire of porcine bone marrow.


[New perspectives for the diagnosis and prognosis of Graves' disease].
Graves' thyroiditis is a frequent disease. It has a considerable impact on patient's life quality and health expenses. Thus, it is important to optimize the treatment and follow up. The identification of new factors predisposing to the disease and factors that may help to predict the severity or recurrence of the disease or the occurrence of graves' orbitopathy could optimize the management. Genetic predisposition has a major role in the development of Graves' disease. The new genes in addition to those already known to be involved in Graves' disease are under study. However, genetic predisposition alone cannot explain the occurrence of the disease. MicroRNAs are non-genetic factors that are significantly associated with different severities or relapses of Graves' disease as well as with the occurrence of graves' orbitopathy. These genetic and epigenetic factors together with known environmental factors can be used to predict the risk of relapse of Graves' disease or of the occurrence of orbital orbitopathy. This will lead to new promising therapeutic targets.


The starch-binding domain as a tool for recombinant protein purification.
Recombinant protein purification with affinity tags is a widely employed technique. One of the most common tags used for protein purification is the histidine tag (Histag). In this work, we use a tandem starch-binding domain (SBDtag) as a tag for protein purification. Four proteins from different sources were fused to the SBDtag, and the resulting fusion proteins were purified by affinity chromatography using the Histag or the SBDtag. The results showed that the SBDtag is superior to the Histag for protein purification. The efficient adsorption of the fusion proteins to raw corn starch was also demonstrated, and two fusions were selected to test purification directly using raw starch from rice, corn, potato, and barley. The two fusion proteins were successfully recovered from crude bacterial extract using raw starch, thus demonstrating that the SBDtag can be used as an efficient affinity tag for recombinant protein purification on an inexpensive matrix.


Epoxidation of the methamphetamine pyrolysis product, trans-phenylpropene, to trans-phenylpropylene oxide by CYP enzymes and stereoselective glutathione adduct formation.
Pyrolytic products of smoked methamphetamine hydrochloride are well established. Among the various degradation products formed, trans-phenylpropene (trans-beta-methylstyrene) is structurally similar to styrene analogues known to be bioactivated by CYP enzymes. In human liver microsomes, trans-phenylpropene was converted to the epoxide trans-phenylpropylene oxide (trans-2-methyl-3-phenyloxirane) and cinnamyl alcohol. Incubation of trans-phenylpropene with microsomes in the presence of enzyme-specific P450 enzyme inhibitors indicated the involvement of CYP2E1, CYP1A2, and CYP3A4 enzymes. Both (R,R)-phenylpropylene oxide and (S,S)-phenylpropylene oxide were formed in human liver microsomal preparations. Enantiomers of trans-phenylpropylene oxide were stereoselectively and regioselectively conjugated in a Phase II drug metabolism reaction catalyzed by human liver cytosolic enzymes consisting of conjugation with glutathione. The structure of the phenylpropylene oxide-glutathione adduct is consistent with nucleophilic ring-opening by attack at the benzylic carbon. Exposure of cultured C6 glial cells to (S,S)-phenylpropylene oxide produced a cytotoxic response in a concentration-dependent manner based on cell degeneration and death.


[Features of the structure of the spleen and lymph nodes of the rat after exposure to the secretion of the suprascapular gland of the toad].
Zootoxin of the toad suprascapular glands in the dose of 0.5 mg/kg of the body mass (rat) increases mitotic activity of lymphoid cells in B-dependent zones of the lymph nodes and spleen. Simultaneously, mitotic activity of cells in T-dependent zones of the spleen also increases.


Clotrimazole, but not dexamethasone, is a potent in vitro inhibitor of cytochrome P450 isoforms CYP1A and CYP3A in rainbow trout.
The effects of clotrimazole (CLO) and dexamethasone (DEX), both detected in the aquatic environment, were assessed on inhibition of cytochrome P450 (CYP450) in hepatic microsomes of rainbow trout. Activity of three CYP450 isoforms: ethoxyresorufin O-deethylase (EROD; CYP1A), 7-benzyloxy-4-trifluoromethylcoumarin O-debenzylase (BFCOD; CYP3A) and p-nitrophenol hydroxylase (PNPH; CYP2E1-like protein) was investigated in the presence of four concentrations of CLO and DEX. Clotrimazole in a concentration range of 1-100μM decreased the activity of EROD and BFCOD. The inhibition was reversible, as pre-incubation of the microsomes with CLO, before addition of the substrate, had no effect. EROD activity was non-competitively inhibited with a Ki of 0.5μM, and BFCOD activity revealed competitive inhibition with a Ki of 0.04μM. The relatively low Ki for CLO inhibition of EROD and BFCOD activity may indicate that the ability of CYP1A and CYP3A to metabolize xenobiotics is reduced in the presence of CLO. PNPH activity was not affected by CLO. DEX showed no inhibitory potency on any investigated reaction. CLO, but not DEX, inhibited EROD and BFCOD activity by different mechanisms.


Oligonucleotide-functionalized silver nanoparticle extraction and laser-induced fluorescence for ultrasensitive detection of mercury(II) ion.
This study describes the development of a simple, sensitive, and selective detection system for Hg(2+) ion by combining nanoparticle extraction, fluorescent dye labeling, and flow injection analysis (FIA) detection. Repeats of 33 thymine nucleotides-functionalized silver nanoparticles (T(33)-AgNPs) specifically capture Hg(2+) from aqueous solution through the coordination between T(33) and Hg(2+). Meanwhile, Hg(2+) ion drives a T(33) conformational change from a random coil to a folded structure. The T(33)-Hg(2+)complexes adsorbed on the NP surface were collected from the initial sample by centrifugation, and they were then detached from the NP surface by addition of H(2)O(2). The T(33)-Hg(2+) complexes preferentially bind to SYBR Green I (SG), enhancing the SG fluorescence. By contrast, SG fluoresces only weakly in the presence of T(33) alone. The extraction efficiency of Hg(2+) was highly dependent on polythymine length, the concentration of T(33)-AgNPs, and the incubaton time of T(33)-AgNPs with Hg(2+). Under optimal extraction and labeling conditions, FIA detection showed the limit of detection (at a signal-to-noise ratio of three) for Hg(2+)of 3 pM. The selectivity of our analytical system is more than 1000-fold for Hg(2+) over any metal ions. We validated the applicability of this system for the determination of Hg(2+) concentrations in tap water.


Value of machine learning algorithms for predicting diabetes risk: A subset analysis from a real-world retrospective cohort study.
To compare the application value of different machine learning (ML) algorithms for diabetes risk prediction. This is a 3-year retrospective cohort study with a total of 3,687 participants being included in the data analysis. Modeling variable screening and predictive model building were carried out using logistic regression (LR) analysis and 10-fold cross-validation, respectively. In total, six different ML algorithms, including random forests, light gradient boosting machine, extreme gradient boosting, adaptive boosting (AdaBoost), multi-layer perceptrons and gaussian naive bayes were used for model construction. Model performance was mainly evaluated by the area under the receiver operating characteristic curve. The best performing ML model was selected for comparison with the traditional LR model and visualized using Shapley additive explanations. A total of eight risk factors most associated with the development of diabetes were identified by univariate and multivariate LR analysis, and they were visualized in the form of a nomogram. Among the six different ML models, the random forests model had the best predictive performance. After 10-fold cross-validation, its optimal model has an area under the receiver operating characteristic value of 0.855 (95% confidence interval [CI] 0.823-0.886) in the training set and 0.835 (95% CI 0.779-0.892) in the test set. In the traditional LR model, its area under the receiver operating characteristic value is 0.840 (95% CI 0.814-0.866) in the training set and 0.834 (95% CI 0.785-0.884) in the test set. In the real-world epidemiological research, the combination of traditional variable screening and ML algorithm to construct a diabetes risk prediction model has satisfactory clinical application value.


Constituents of West African medicinal plants XX: quindoline from Cryptolepis sanguinolenta.
An ethanol extract of the roots of Cryptolepis sanguinolenta (Asclepidaceae) afforded, after partitioning and chromatography, the alkaloids quindoline and CSA-3. The structural elucidation of CSA-3 is currently in progress. Cryptolepine was also isolated from this species.


Decrease of functional coupling between left and right auditory cortices during dichotic listening: an electroencephalography study.
The present study focused on functional coupling between human bilateral auditory cortices and on possible influence of right over left auditory cortex during dichotic listening of complex non-verbal tones having near (competing) compared with distant non-competing fundamental frequencies. It was hypothesized that dichotic stimulation with competing tones would induce a decline of functional coupling between the two auditory cortices, as revealed by a decrease of electroencephalography coherence and an increase of directed transfer function from right (specialized for the present stimulus material) to left auditory cortex. Electroencephalograph was recorded from T3 and T4 scalp sites, overlying respectively left and right auditory cortices, and from Cz scalp site (vertex) for control purposes. Event-related coherence between T3 and T4 scalp sites was significantly lower for all electroencephalography bands of interest during dichotic listening of competing than non-competing tone pairs. This was a specific effect, since event-related coherence did not differ in a monotic control condition. Furthermore, event-related coherence between T3 and Cz and between T4 and Cz scalp sites showed no significant effects. Conversely, the directed transfer function results showed negligible influence at group level of right over left auditory cortex during dichotic listening. These results suggest a decrease of functional coupling between bilateral auditory cortices during competing dichotic stimuli as a possible neural substrate for the lateralization of auditory stimuli during dichotic listening.


Monitoring of plasma levels of activated protein C using a clinically applicable oligonucleotide-based enzyme capture assay.
Human-activated protein C (APC) is a serine protease with anticoagulant, anti-inflammatory and cytoprotective functions. This feature renders APC to be a promising vascular-inflammatory biomarker. The aim of the present study was the development and validation of a technique that allows the measurement of APC plasma levels under practical laboratory conditions. Based on the APC-binding ssDNA aptamer HS02-52G we developed an oligonucleotide-based enzyme capture assay (OECA) that quantifies aptamer-captured APC through hydrolysis rates of a fluorogenic peptide substrate. After optimization of pre-analytical conditions, plasma APC levels were measured in healthy individuals and patients undergoing hip replacement surgery. A combination of APC-OECA with an aprotinin-based quenching strategy allowed APC analysis with a limit of detection as low as 0.022 ± 0.005 ng mL(-1) (0.39 ± 0.10 pmol L(-1)) and a limit of quantification of 0.116 ± 0.055 ng mL(-1) (2.06 ± 0.98 pmol L(-1)). While APC plasma levels in healthy individuals fell below the quantifiable range of the APC-OECA platform, levels substantially increased in patients undergoing hip replacement surgery reaching peak values of up to 12 ng mL(-1) (214 pmol L(-1)). When normalized to the amount of thrombin generated, interindividual variabilities in the APC generating capacity were observed. In general, with a turn-around time from blood sampling to generation of test results of < 7 h, the APC-OECA platform allows sensitive and rapid determination of circulating APC levels under pathological conditions.


Dobzhansky and Montagu's Debate on Race: The Aftermath.
Dobzhansky and Montagu debated the use and validity of the term "race" over a period of decades. They failed to reach an agreement, and the "debate" has continued to the present. The ms contains an account of the debate to the present. This essay is part of a Special Issue, Revisiting Garland Allen's Views on the History of the Life Sciences in the Twentieth Century.


The effect of pregnancy on the interval between one oestrus and the next in the tammar wallaby, Macropus eugenii.
In females mated to vasectomized males the interval between oestrous periods was 30.6 +/- 1.17 (s.d.) days but when mated to intact males and pregnancy intervened, it was 29. +/- 1.26 days (P less than 0.0025). After removal of pouch young, females carrying diapausing blastocysts gave birth 26.2 days later and came into oestrus 26.4 +/- 0.57 (s.d.) days later. When post-partum fertilization was prevented, removal of pouch young was followed by oestrus 30.4 +/- 0.99 days later (P less than 0.0005). These results indicate an influence of the conceptus upon the oestrous cycle of Macropus eugenii.


Differentiation of benign and malignant breast lesions: a comparison between automatically generated breast volume scans and handheld ultrasound examinations.
To assess the diagnostic value of automated breast volume scanning (ABVS) or conventional handheld ultrasonography (HHUS) for the differentiation of benign and malignant breast lesions. The study prospectively evaluated 239 lesions in 213 women who were scheduled for open biopsy. The patients underwent ABVS and conventional HHUS. The sensitivity, specificity, accuracy, false positive rate, false negative rate, and positive and negative predictive values for HHUS and ABVS images were calculated using histopathological examination as the gold standard. Additionally, diagnostic accuracy was further evaluated according to the size of the masses. Among the 239 breast lesions studied, pathology revealed 85 (35.6%) malignant lesions and 154 (64.4%) benign lesions. ABVS was similar to HHUS in terms of sensitivity (95.3% vs. 90.6%), specificity (80.5% vs. 82.5%), accuracy (85.8% vs. 85.3%), positive predictive value (73.0% vs. 74.0%), and negative predictive value (93.3% vs. 94.1%). The area under the receiver operating characteristic (ROC) curve, which is used to estimate the accuracy of the methods, demonstrated only minor differences between HHUS and ABVS (0.928 and 0.948, respectively). The diagnostic accuracy of HHUS and ABVS in differentiating benign from malignant breast lesions is almost identical. However, ABVS can offer new diagnostic information. ABVS may help to distinguish between real lesions and inhomogeneous areas, find small lesions, and demonstrate the presence of intraductal lesions. This technique is feasible for clinical applications and is a promising new technique in breast imaging.


Applying social science research to solving problems of modern nations: problems and pitfalls.
The reasons the social sciences do not have an effect on public policies are discussed, including that there are conflicting results, the effect of scholars' biases on their research, and a desire to keep the social sciences value-free.


Pre-trial fronto-occipital electrophysiological connectivity affects perception-action integration in response inhibition.
Inhibition of inappropriate behavior is relevant in many everyday situations. Nevertheless, the mechanisms that induce response inhibition based on sensory information and what influences these mechanisms are not entirely understood. We examined neurophysiological processes of perception-action integration in response inhibition and the impact of the pre-trial neurophysiological functional connectivity state in the theta and alpha band on these integration processes. The study was motivated by the Theory of Event Coding framework. Within the trial, fronto-medial theta band activity and occipital alpha band activity revealed an opposing interplay depending on the necessity of (re-)binding event files, i.e., the disintegration and recombination of stimulus-response associations, during response inhibition. When response inhibition required the reconfiguration of event files, this was associated with increased theta band activity but lower alpha band activity, and vice versa for the retrieval of event files. Notably, the most substantial impact of pre-trial connectivity on the within-trial event file binding effect (the difference between conditions that require reconfiguration and those that do not) during response inhibition occurred between fronto-medial areas and areas of the ventral stream in the theta frequency band. This suggests a preparatory top-down control of sensory areas before stimulus presentation. Increased pre-trial connectivity was associated with a decreased event file binding effect in the alpha frequency band and an increased event file binding effect in the theta frequency band during response inhibition. This implies an impact of the pre-trial functional connectivity state on inhibitory gating processes of relevant information and event file (re-)binding during response inhibition. The study shows how perception-action integration during response inhibition is affected by preceding transient neurophysiological connectivity states.


Rural disparities in surgical care from gynecologic oncologists among Midwestern ovarian cancer patients.
Up to one-third of women with ovarian cancer in the United States do not receive surgical care from a gynecologic oncologist specialist despite guideline recommendations. We aim to investigate the impact of rurality on receiving surgical care from a specialist, referral to a specialist, and specialist surgery after referral, and the consequences of specialist care. We utilized a retrospective cohort created through an extension of standard cancer surveillance in three Midwestern states. Multivariable adjusted logistic regression was utilized to assess gynecologic oncologist treatment of women 18-89 years old, who were diagnosed with primary, histologically confirmed, malignant ovarian cancer in 2010-2012 in Kansas, Missouri and Iowa by rurality. Rural women were significantly less likely to receive surgical care from a gynecologic oncologist specialist (adjusted odds ratio (OR) 0.37, 95% confidence interval (CI) 0.24-0.58) and referral to a specialist (OR 0.37, 95% CI 0.23-0.59) compared to urban women. There was no significant difference in specialist surgery after a referral (OR 0.56, 95% CI 0.26-1.20). Rural women treated surgically by a gynecologic oncologist versus non-specialist were more likely to receive cytoreduction and more complete tumor removal to ≤1 cm. There is a large rural-urban difference in receipt of ovarian cancer surgery from a gynecologic oncologist specialist (versus a non-specialist). Disparities in referral rates contribute to the rural-urban difference. Further research will help define the causes of referral disparities, as well as promising strategies to address them.


New 24-membered macrolides SPA-6952A and B produced by Streptomyces sp.
Two new 24-membered macrolides, SPA-6952A and B, were isolated from the fermentation broth of Streptomyces sp. SPA-6952. The structures of the new macrolides were determined by spectral analyses, including 2D NMR techniques. These compounds exhibited cytotoxic activity against human promyelocytic leukemia HL-60 cells.


Obesity as a Risk Factor in Cosmetic Abdominal Body Contouring: A Systematic Review and Meta-Analysis.
The incidence of obesity is on the rise around the globe. Outside of the massive weight loss (MWL) patient population, knowledge of risk factors associated with abdominal body contouring (BC) is limited. This systematic review and meta-analysis assesses the impact of obesity has on cosmetic abdominal BC outcomes. A systematic review conducted in accordance with PRISMA 2020 was done. PubMed, Embase, Scopus, and COCHRANE databases were reviewed under search syntax "obesity," "abdominoplasty," "panniculectomy," and "body contouring" for articles. Cosmetic was defined as abdominoplasty or panniculectomy outside the context of MWL. Obesity was defined as BMI ≥ 30 kg/m2. Studies reporting postoperative outcomes with less than 50% of their population involving MWL patients were included. Postoperative outcomes were assessed by pooled analysis and meta-analysis. Of 3088 initial studies, 16 met inclusion criteria, and nine were used for pooled and meta-analysis. Meta-analysis demonstrated that obesity was associated with more seromas (OR 1.45, 1.06-1.98, p = 0.02), hematomas (OR 2.21, 1.07-4.57, p = 0.03), and total surgical site occurrences (OR 1.99, 1.30-3.04, p = 0.0016). There was no significant difference in odds of any other complications. Analysis by obesity class showed no significant increase in odds in seromas or wound dehiscence. This review demonstrates obesity increased odds of postoperative complications following cosmetic abdominal BC. However, risk of complications does not continue to increase with higher obesity class. A BMI ≥ 30 kg/m2 should not be a strict contraindication to cosmetic abdominal BC. Instead, plastic surgeons should evaluate patients on a case-by-case basis. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .


Three-dimensional fluid-attenuated inversion recovery magnetic resonance imaging findings and prognosis in sudden sensorineural hearing loss.
Three-dimensional fluid-attenuated inversion recovery (3D-FLAIR) magnetic resonance imaging (MRI) has recently been developed to detect high concentrations of protein or hemorrhage. We have previously reported that 50% of patients with sudden sensorineural hearing loss (SNHL) show high signals in the affected inner ear on 3D-FLAIR MRI. However, the relationship between 3D-FLAIR findings and hearing prognosis is unclear. Our objective was to evaluate the relationship between the results of 3D-FLAIR MRI at 3 Tesla and prognosis in sudden SNHL. We used 3D-FLAIR at 3 Tesla with and without gadolinium enhancement to evaluate the pathologic conditions in the inner ears of 48 patients with sudden SNHL. Thirty-one of 48 patients with sudden SNHL showed high signals in the affected inner ear on precontrast 3D-FLAIR. Hearing improvement in patients with high signals in the affected inner ear on precontrast 3D-FLAIR (25 +/- 19 dB) was significantly worse than that in patients with no signal (45 +/- 27 dB; P < .05). Our analysis suggests that high signals in the affected inner ear on precontrast 3D-FLAIR MRI is a new prognostic factor for sudden SNHL. 3D-FLAIR findings show that high signals in the cochlea on precontrast 3D-FLAIR are related to a poor hearing prognosis. These signals may reflect minor hemorrhage or an increased concentration of protein in the inner ear, which has passed through blood vessels with increased permeability or has originated in disrupted cells in the inner ear.


Development of a high-throughput yeast two-hybrid screening system to study protein-protein interactions in plants.
We have developed a high-throughput yeast two-hybrid screening system (HTP-YTH) that incorporates yeast gap-repair cloning, multiple positive ( ADE2, HIS3, lacZ) and negative ( URA3-based) selection schemes to reduce the incidence of negative and false positive clones, and automation of laboratory procedures to increase throughput. This HTP-YTH system has been applied to the study of protein-protein interactions that are involved in rice defense signal transduction pathways. More than 100 genes involved in plant defense responses were selected from DuPont's rice expressed sequence tag (EST) databases as baits for HTP-YTH screening. Results from YTH screening of eight of these rice genes are presented in this paper. Not only have we identified known protein-protein interactions, but we have also discovered novel interactions, which may ultimately reveal the regulatory network of host defense signal transduction pathways. We have demonstrated that our HTP-YTH method can be used to map protein-protein interaction networks and signal transduction pathways in any system. In combination with other approaches, such efficient YTH screens can help us systemically to study the functions of known and unknown genes in the genomics era.


Actin antibodies. Preparation and characterization of antibodies specific for smooth-muscle actin isoforms.
We have determined the specificity of sera elicited by glutaraldehyde-stabilized bovine aortic actin. This modification induces a high titre of antibodies directed against the N-terminal (residues 1-39) and the C-terminal region of smooth-muscle actins. The crude antisera were purified on peptide (corresponding to the 1-9 or 1-8 N-terminal sequences of smooth-muscle isoactins)-polyacrylic-resin columns. By fractionating the antisera we obtained oligoclonal antibody populations specific for each isoactin.


NMR reveals a novel glutaredoxin-glutaredoxin interaction interface.
Glutaredoxins (Grx) represent a large family of glutathione (GSH)-dependent oxidoreductases that catalyse the reduction of disulfides or glutathione mixed disulfide. Grx domains from pathogenic bacteria and plant Grxs have been recently reported to target specific peroxiredoxins (Prxs). The specificity that triggers the interaction between Grx and Prx is poorly understood and is only based on the structure of Haemophilus influenzae Prx-Grx hybrid (hyPrx5). We report here an NMR study of the Populus tremula Grx C4 that targets a P.tremula D-type II Prx. We show that Grx C4 specifically self-associates in a monomer-dimer equilibrium with an apparent K(d) of ca 2.6 mM. Grx C4 homodimer was docked under experimental restraints. The results reveal a novel Grx-Grx interface that is unrelated to the hyPrx5 Grx-Grx dimer interface. Chemical-shift perturbations and 15N spin-relaxation measurements show that the auto-association surface comprises both the active site and the GSH binding site. Reduced GSH is demonstrated to bind reduced Grx with a K(d) of ca 8.6 mM. The potential biological significance of the new Grx-Grx interaction interface is discussed.


Stability of cyflumetofen resistance in Tetranychus cinnabarinus and its correlation with glutathione-S-transferase gene expression.
Cyflumetofen is an outstanding acaricide with a novel mode of action. Tetranychus cinnabarinus, an important agricultural pest, is notorious for developing resistance to most classes of acaricides rapidly and results in enormous loss for the economy. Our previous study had pointed out glutathione S-transferase (GSTs) significantly contributed to the cyflumetofen-resistance formation in T. cinnabarinus, but the more specific mechanism needed to be further investigated. The unstable resistance was observed in cyflumetofen-resistant strain (CyR)under acaricide-free condition. The activity of GSTs increased along with the development of resistance. The expressions of 13 GST genes were detected in CyR and susceptible strain (SS), of which six genes were overexpressed in CyR and the TcGSTm02 was selected as the representative for functional study. The expression of TcGSTm02 changed along with the resistant level of CyR with the same trend. Recombinant protein of TcGSTm02 with high activity was successfully obtained by E. coli expression system, whose activity could be inhibited by cyflumetofen (IC50 = 0.23 mM). Recombinant TcGSTm02 could effectively decompose cyflumetofen, and catalyze GS- to conjugate with cyflumetofen. All clues confirmed that GSTs strongly associated with cyflumetofen-resistance and a representative gene, TcGSTm02, showed function on contributing the evolution of cyflumetofen-resistance in T. cinnabarinus. © 2019 Society of Chemical Industry.


Resource utilization of sympatric and experimentally allopatric cutthroat trout and Dolly Varden charr.
Resource utilization by cutthroat trout (CT) and Dolly Varden charr (DV) was studied 8 years after experimental transfers from sympatry had established reproducing allopatric populations in two nearby fishless lakes. Allopatric DV significantly increased their utilization of shallow-dwelling zoobenthos, and increased their vertical distribution in comparison to that in sympatry. In contrast, allopatric CT showed little change in the proportions of major prey types utilized, and, if anything, restricted their vertical distribution in comparison to that in sympatry. The results can be explained by the hypothesis that the resource use of DV is strongly influenced by interspecific competition from CT, whereas CT largely remains unaffected by this interaction. An alternative hypothesis, that lake differences can explain the differences in resource use between sympatry and allopatry, was evaluated by comparing food resource availability and other biotic and abiotic characteristics of the three study lakes. None of these could account for the shift in resource use by DV between sympatry and allopatry, but lake differences may explain why allopatric CT showed a restricted habitat use in comparison with their sympatric donor stock. The results of this whole-lake transfer experiment are consistent with earlier reported field and laboratory studies, and suggest that the aggressive dominance of CT is the most important mechanism by which DV are displaced from littoral and near-surface habitats in sympathy with CT.


[Liver and intestinal transplant in paediatric population].
Our organizational model allows an annual 1,000 liver transplants. Pediatric liver transplantation constitutes 5% of such activity and provides, in children with severe, progressive and irreversible liver disease, a 1 year-survival of 90% and more than 80% after 15 years of follow-up. The main indication is biliary atresia followed by metabolic liver disease and acute liver failure. Around half of the procedures are performed in children under two years and 25-30% in the first year of life. The waiting list remains at around 35 patients, with an average of 100 patients enrolled annually and 60 of them finally transplanted after an average of 136.3 days on the waiting list. The prioritization of the candidates uses the PELD as an objective tool for decision-making. However, the progressive aging of donors, with a profile increasingly different from the requirements of the pediatric patients included in the waiting list, requires strategies such as living donor liver transplantation and the split liver transplantation, to increase the probability of transplant while reducing both time and mortality on the waiting list at the same time. Pediatric intestinal transplantation registers a low indication but involves strict requirements that outline a very uncommon donor in our country which, together with the absence of alternatives that outweigh the impact of these difficulties, penalizes the chances of transplant for these patients.


Long-term clinical and functional impact of biliopancreatic diversion on type 2 diabetes in morbidly and non-morbidly obese patients.
Obesity surgery has been proposed as a treatment option for diabetic patients with body mass index (BMI)<35 kg/m(2), but the efficacy of metabolic surgery has not been conclusively determined. To evaluate the long-term metabolic outcome of non-morbidly obese (NMO) patients with type 2 diabetes (T2D) after biliopancreatic diversion (BPD). Two groups of T2D patients with different degree of obesity (NMO, 17 cases, BMI 25-35 kg/m(2); and morbidly obese [MO], 13 cases, BMI>35 kg/m(2)) were studied before and at 1 and 5 years after BPD in a university hospital setting. Insulin secretion was assessed by acute insulin response (AIR) to intravenous glucose and by insulinogenic index (IGI). In all MO patients, T2D was remitted or controlled (1 case) at 1 year and results were maintained at 5 years; AIR (μU/mL) and IGI (μU/mg) improved (P<.001) at 1 year (from .1±3.1 to 18.52±21.9, and from 6.0±8.5 to 9.1±22.8, respectively) with a further increase (to 24.8±25.5 and to 14.3±13.8, respectively) at 5 years. Within the NMO group, T2D was remitted in 1/17 and controlled in 14/17 patients at 1 year, and in 2/17 and in 4/17 patients at 5 years, respectively; AIR (μU/mL) and IGI (μU/mg) remained unchanged throughout the postoperative period (from .31±9.26 to 1.5±2.8 at 1 yr and to .4±3.29 at 5 yr for AIR, and from 2.2±4.9 to 1.3±9.0 at 1 yr and to 2.3±3.3 at 5 yr for IGI). After BPD, restoration of β-cell secretion/production plays a pivotal role in determining postoperative T2D remission.


Associations between Milk Fatty Acid Profile and Body Condition Score, Ultrasound Hepatic Measurements and Blood Metabolites in Holstein Cows.
Dairy cows have high incidences of metabolic disturbances, which often lead to disease, having a subsequent significant impact on productivity and reproductive performance. As the milk fatty acid (FA) profile represents a fingerprint of the cow's nutritional and metabolic status, it could be a suitable indicator of metabolic status at the cow level. In this study, we obtained milk FA profile and a set of metabolic indicators (body condition score, ultrasound liver measurements, and 29 hematochemical parameters) from 297 Holstein-Friesian cows. First, we applied a multivariate factor analysis to detect latent structure among the milk FAs. We then explored the associations between these new synthetic variables and the morphometric, ultrasonographic and hematic indicators of immune and metabolic status. Significant associations were exhibited by the odd-chain FAs, which were inversely associated with β-hydroxybutyrate and ceruloplasmin, and positively associated with glucose, albumin, and γ-glutamyl transferase. Short-chain FAs were inversely related to predicted triacylglycerol liver content. Rumen biohydrogenation intermediates were associated with glucose, cholesterol, and albumin. These results offer new insights into the potential use of milk FAs as indicators of variations in energy and nutritional metabolism in early lactating dairy cows.


Acceleration of ASL-based time-resolved MR angiography by acquisition of control and labeled images in the same shot (ACTRESS).
Noncontrast 4D-MR-angiography (MRA) using arterial spin labeling (ASL) is beneficial because high spatial and temporal resolution can be achieved. However, ASL requires acquisition of labeled and control images for each phase. The purpose of this study is to present a new accelerated 4D-MRA approach that requires only a single control acquisition, achieving similar image quality in approximately half the scan time. In a multi-phase Look-Locker sequence, the first phase was used as the control image and the labeling pulse was applied before the second phase. By acquiring the control and labeled images within a single Look-Locker cycle, 4D-MRA was generated in nearly half the scan time of conventional ASL. However, this approach potentially could be more sensitive to off-resonance and magnetization transfer (MT) effects. To counter this, careful optimizations of the labeling pulse were performed by Bloch simulations. In in-vivo studies arterial visualization was compared between the new and conventional ASL approaches. Optimization of the labeling pulse successfully minimized off-resonance effects. Qualitative assessment showed that residual MT effects did not degrade visualization of the peripheral arteries. This study demonstrated that the proposed approach achieved similar image quality as conventional ASL-MRA approaches in just over half the scan time. Magn Reson Med 79:224-233, 2018. © 2017 International Society for Magnetic Resonance in Medicine.


Predictors of improvement in left ventricular function after initially successful angioplasty of unprotected left main coronary artery stenoses.
Percutaneous coronary intervention (PCI) has been increasingly applied to unprotected left main coronary artery (LMCA) lesions, with varied procedural success and clinical outcomes. However, the effect of PCI on left ventricular performance is still unclear, and there are no clinical studies assessing factors that influence left ventricular ejection fraction (LVEF) in these cases. Between April 1986 and August 2002, de novo PCI was performed for unprotected LMCA stenoses in 199 patients. Close clinical and angiographic follow-up were conducted after the procedure. One hundred eighty patients survived over six months and analysis of paired left ventriculography was possible in 175 patients. Improvement in LVEF was observed in the entire population (52.9 +/- 15.7% to 56.1 +/- 14.3%, p = 0.048). The LVEF change was 6.7 +/- 9.5% (p < 0.01) in group with baseline LVEF < or = 50% and 0.7 +/- 6.7 % (p = NS) in group with LVEF > 50%. There was significant intergroup difference (p < 0.001). Patients with baseline diameter stenosis > or = 60% had an improvement of 5.3 +/- 8.3% (p < 0.05) whereas those with stenosis < 60% had no improvement (2.0 +/- 8.4%, p = NS). CK-MB elevation > or = 3 times normal after PCI had a significant inverse association with improvement in LVEF (p < 0.05). Multivariate analysis revealed baseline LVEF < or = 50% was the only independent predictor of improvement in LVEF (standard estimate = 3.509, 95% CI: 2.164-4.854, p < 0.001). Successful PCI procedure is associated with significant improvement in LVEF, especially in patients with depressed left ventricular function.


An expanding role for antithrombotic therapy in cancer patients.
Thromboembolic disease has a negative impact on outcome in the cancer patient. Venous thromboembolism appears to be a more aggressive disease in patients with cancer who present with both a greater thrombus burden and more pronounced derangements of coagulation parameters with non-cancer patients with thrombosis. Restrospective analyses suggest that treatment of venous thromboembolism in cancer patients with low-molecular-weight heparin is associated with additional benefits in terms of their survival. The mechanism for this effect is unclear but may be attributed to a reduced incidence of recurrent thrombosis and fatal pulmonary embolism in those patients with underlying thrombosis. To investigate and potential of low-molecular-weight heparin to prolong survival in cancer patients without underlying thrombosis, a prospective trial (FAMOUS) has evaluated the effect of treatment with the low-molecular-weight heparin dalteparin for 1 year terms of survival of patients with advanced cancer and no underlying thrombosis.


In vitro and in vivo investigations on the antitumour activity of Chelidonium majus.
Chelidonium majus L. (Papaveraceae) (greater celandine) is a medicinal herb that is widely spread in Europe. Antitumoural activity has been reported for C. majus extracts. To investigate the antitumour activity of a C. majus extract in vitro and in vivo. Cytotoxic effects of C. majus extracts were evaluated on human cancer cell lines, i.e. PANC-1 (pancreas cancer), HT-29 (colon cancer), MDA-MB-231 (breast cancer), PC-EM005 and PC-EM002 (primary endometrium cancer cells), and PANC02 (murine pancreatic adenocarcinoma cells). A preliminary in vivo study was performed to evaluate the effect of a defatted C. majus extract and Ukrain(TM) in a highly metastatic murine pancreatic model. Chelidonium majus L. herb containing 1.26% (dry weight) of total alkaloids expressed as chelidonine was used to prepare an 80% ethanolic extract (CM2). This crude extract was then defatted with n-hexane, resulting in a defatted C. majus extract (CM2B). Cytotoxic effects of the two extracts (CM2 and CM2B) were evaluated on human and murine cell lines in vitro. CM2B and Ukrain(TM) were evaluated in a highly metastatic murine pancreatic model. Four main benzylisoquinoline alkaloids were identified in CM2B, i.e. chelidonine, sanguinarine, chelerythrine and protopine, using HPLC-UV. CM2 showed a high cytotoxic activity against PANC-1 (IC50, 20.7 µg/ml) and HT-29 (IC50, 20.6 µg/ml), and a moderate cytotoxic activity against MDA-MB-231 (IC50, 73.9 µg/ml). CM2 as well as CM2B showed a moderate to high cytotoxic activity against the PANC02 cell line (IC50, 34.4 and 36.0 µg/ml). Low to almost no cytotoxic effect was observed on primary endometrium cancer cells PC-EM005, PC-EM002 and on normal fibroblast cells 3T3, when treated with CM2B. Significantly less metastases were counted in mice treated with 1.2 mg/kg CM2B, but not with 3.6 mg/kg Ukrain(TM), compared to the control group. The extract, however, did not affect the weight of the primary tumours.


Leishmania DNA Replication Timing: A Stochastic Event?
For eukaryotic genomes, DNA synthesis initiates at multiple discrete regions known as replication origins in a dynamic yet regulated manner to ensure genomic stability. Two recent studies using different approaches reveal few Leishmania origins and that origin firing may proceed in a mainly stochastic manner.


Secondary structure and protein deamidation.
The deamidation reactions of asparagine residues in alpha-helical and beta-turn secondary structural environments of peptides and proteins are reviewed. Both kinds of secondary structure tend to stabilize asparagine residues against deamidation, although the effects are not large. The effect of beta-sheet structures on asparagine stability is unclear, although simple considerations suggest a stabilization in this environment also.


Emergence of a VIM-2-producing extensively drug-resistant (XDR) Pseudomonas aeruginosa ST309 in South America: a comparative genomic analysis.
Pseudomonas aeruginosa is considered a top priority pathogen associated with elevated morbidity and mortality. Worldwide outbreaks have been associated with a few high-risk epidemic P. aeruginosa lineages. However, the biological features involved in the persistence and spread of such lineages in clinical settings remain to be unravelled. This study reports the emergence of an extensively drug-resistant (XDR) sequence type 309 (ST309) P. aeruginosa in South America (Brazil), specifically in the Amazon region. Genomic analyses were performed with 42 complete and draft ST309 genomes, giving insights into its epidemiology, resistome and mobilome. A heterogeneous distribution of acquired antimicrobial resistance genes among ST309 genomes was observed, which included blaVIM-2, blaIMP-15 and qnrVC1, all associated with class 1 integrons. Mobilome mining showed the presence of integrative and conjugative elements (ICEs), transposons and genomic islands (GIs) harbouring a huge arsenal of heavy metal resistance determinants, which probably provided adaptive advantages to the ST309 lineage.


Expression and characterization of a lytic polysaccharide monooxygenase from Bacillus thuringiensis.
Lytic polysaccharide monooxygenases (LPMOs) are recently discovered oxidative enzymes that are capable of oxidative cleavage of recalcitrant polysaccharides such as chitin or cellulose. Despite the importance of LPMOs in biomass conversion and the large number of lpmo genes in microorganisms, only a few LPMOs have been well studied, and further characterization of these proteins is thus of interest. In this study, a chitin-active AA10 family LPMO from Bacillus thuringiensis subsp. kurstaki, BtLPMO10A, is described. This enzyme generates even-numbered oxidized oligosaccharides as the dominated products from crystalline chitin, however, interestingly, when colloidal chitin is used as the substrate, a ladder of oxidized oligosaccharides is observed. These results provide new insights into the action mode of LPMOs that may be affected by the substrates.


Pentaethylenehexamine-Loaded Hierarchically Porous Silica for CO₂ Adsorption.
Recently, amine-functionalized materials as a prospective chemical sorbent for post combustion CO₂ capture have gained great interest. However, the amine grafting for the traditional MCM-41, SBA-15, pore-expanded MCM-41 or SBA-15 supports can cause the pore volume and specific surface area of sorbents to decrease, significantly affecting the CO₂ adsorption-desorption dynamics. To overcome this issue, hierarchical porous silica with interparticle macropores and long-range ordering mesopores was prepared and impregnated with pentaethylenehexamine. The pore structure and amino functional group content of the modified silicas were analyzed by scanning electron microscope, transmission electron microscope, N₂ adsorption, X-ray powder diffraction, and Fourier transform infrared spectra. Moreover, the effects of the pore structure as well as the amount of PEHA loading of the samples on the CO₂ adsorption capacity were investigated in a fixed-bed adsorption system. The CO₂ adsorption capacity reached 4.5 mmol CO₂/(g of adsorbent) for HPS-PEHA-70 at 75 °C. Further, the adsorption capacity for HPS-PEHA-70 was steady after a total of 15 adsorption-desorption cycles.


FPAA-based implementation of fractional-order chaotic oscillators using first-order active filter blocks.
Fractional-order chaotic oscillators (FOCOs) have been widely studied during the last decade, and some of them have been implemented on embedded hardware like field-programmable gate arrays, which is a good option for fast prototyping and verification of the desired behavior. However, the hardware resources are dependent on the length of the digital word that is used, and this can degrade the desired response due to the finite number of bits to perform computer arithmetic. In this manner, this paper shows the implementation of FOCOs using analog electronics to generate continuous-time chaotic behavior. Charef's method is applied to approximate the fractional-order derivatives as a ratio of two polynomials in the Laplace domain. For instance, two commensurate FOCOs are the cases of study herein, for which we show their dynamical analysis by evaluating their equilibrium points and eigenvalues that are used to estimate the minimum fractional-order that guarantees their chaotic behavior. We propose the use of first-order all-pass and low-pass filters to design the ratio of the polynomials that approximate the fractional-order. The filters are implemented using amplifiers and synthesized on a field-programmable analog array (FPAA) device. Experimental results are in good agreement with simulation results thus demonstrating the usefulness of FPAAs to generate continuous-time chaotic behavior, and to allow reprogramming of the parameters of the FOCOs.


Interpretation of cosmic X-ray and gamma ray spectra.
Changes in the slope of a measured distribution may be explained partially by the effect of the measurement process itself.


Correction to: Challenges in minor TBI and indications for head CT in pediatric TBI-an update.
The published version of this article unfortunately contained an error.An error in the Abstract was introduced during corrections stage when a "Conclusion" section appended is from a different article and was not provided from authors.


Effects of postpartum acetylsalicylic acid on uterine diseases and reproductive performance in dairy cattle.
Uterine diseases are some of the most prevalent and costly diseases for the dairy industry. It has been reported that an exacerbated and prolonged inflammatory response around calving can increase the risk of developing metritis, which subsequently can impair production and fertility in dairy cows. The objective of this study was to assess the effects of 2 oral administrations of acetylsalicylic acid 24 h apart on the incidence of uterine diseases [i.e., retained fetal membranes, clinical metritis, clinical endometritis) and reproductive performance [i.e., days in milk to conception, number of services to conception, proportion of cows pregnant at first service, proportion of abortion at first service (ABRT)] in dairy cows after calving. Cows from one dairy farm (n = 246) located in central Pennsylvania were included in this study. Animals were blocked by parity and randomly assigned to 1 of 2 groups: (1) ASA (n = 121): within ~12 h after parturition, cows received 2 treatments with acetylsalicylic acid (ASA; 200 mg/kg, 4 boluses) 24 h apart; or (2) UNT (n = 125): cows remained untreated. Clinical metritis was assessed at 7 ± 3 and 14 ± 3 d in milk (DIM) using a Metricheck device (Simcro Tech Ltd., Hamilton, New Zealand). Cows that presented a fetid, red-brownish, watery vaginal discharge were classified as having clinical metritis. Clinical endometritis was assessed through ultrasonography and defined as presence of hyperechoic fluid (>3 mm) in the uterus lumen at 50 ± 10 DIM. The incidence of retained fetal membranes was defined as the failure of expulsion of the placenta within 24 h after calving and recorded by farm personnel. The data were analyzed using the MIXED and GLIMMIX procedures of SAS (SAS Institute Inc., Cary, NC) as a randomized complete block design. A lower proportion of cows treated with acetylsalicylic acid developed clinical metritis at 7 ± 3 DIM (ASA = 34.97 ± 5.57%; UNT = 57.21 ± 5.80%) and tended to develop clinical endometritis at 50 ± 10 DIM (ASA = 3.84 ± 2.67%; UNT = 13.61 ± 5.23%) compared with cows that remained untreated. Cows treated with acetylsalicylic acid tended to require 18 d less to conceive compared with UNT cows (ASA = 125.63 ± 8.21 d; UNT = 143.44 ± 6.28 d). Furthermore, there was a tendency for a treatment × ABRT interaction, whereby ASA cows that aborted at first service tended to conceive 35 d sooner compared with UNT cows that aborted at first service (ASA = 151.42 ± 15.90 d; UNT = 186.38 ± 11.84 d). Although there was no difference in pregnancy per AI at first service between treatment groups (ASA = 23.88 ± 7.92%; UNT = 17.93 ± 6.31%), the proportion of cows aborting at first service in the ASA group tended to be lower than that in the UNT group (ASA = 1.03 ± 1.06%; UNT = 6.04 ± 2.62%). Results from this study suggest that a short-duration acetylsalicylic acid treatment after calving may decrease the incidence of uterine diseases and improve reproductive success in cows that aborted at first service in dairy cattle.


Anterior-Posterior Instability of the Knee Following ACL Reconstruction with Bone-Patellar Tendon-Bone Ligament in Comparison with Four-Strand Hamstrings Autograft.
Purpose. To evaluate anterior-posterior knee laxity using two different autografts. Material-Methods. 40 patients, (34 males and 6 women), 17-54 years old (mean: 31), were included in the present study. Group A (4SHS = 20) underwent reconstruction using four-strand hamstrings, and group B (BPBT = 20) underwent reconstruction using bone-patellar tendon-bone autograft. Using the KT-1000 arthrometer, knee instability was calculated in both knees of all patients preoperatively and 3, 6, and 12 months after surgery at the ACL-operated knee. The contralateral healthy knee was used as an internal control group. Results. Anterior-posterior instability using the KT1000 Arthrometer was found to be increased after ACL insufficiency. The recorded laxity improved after arthroscopic ACL reconstruction in both groups. However, statistically significant greater values were detected in the bone-patellar tendon-bone group, which revealed reduction of anteroposterior stability values to an extent, where no statistical significance with the normal values even after 3 months after surgery was observed. Conclusions. Anterior-Posterior instability of the knee improved significantly after arthroscopic ACL reconstruction. The bone-patellar tendon-bone graft provided an obvious greater stability.


Competition among the pioneers in a seasonal soft-bottom benthic succession: field experiments and analysis of the Gilpin-Ayala competition model.
Controlled experiments, designed to assess the effects of pioneers on succession on an intertidal sandflat, provided evidence for interspecific competition between juvenile Hobsonia florida (Polychaeta, Ampharetidae) and oligochaetes. The field data were fitted to both the linear Volterra and non-linear Gilpin-Ayala competition equations. With its greater number of parameters, the Gilpin-Ayala model must provide a better fit to observed population abundances. The Gilpin-Ayala model is flawed as an explanation of the population trajectories of the H. florida and oligochaetes, because its non-linearity parameter affects only intraspecific competion. With either model our field data demonstrate a solution to Hutchinson's paradox. With competition coefficients near unity and similar carrying capacities, the predicted population trajectories are heavily dependent on initial conditions. The predicted times to competitive exclusion are long and can easily exceed the typical period of environmental constancy. Our study offers evidence for Neill's competitive bottleneck: competition acts primarily on the developmental stages of one of a pair of competing species. The permanent meiofauna may act as a competitive bottleneck for the population growth of benthic macrofauna. The mechanism of this competitive interaction probably involves exploitative interspecific competition for benthic diatoms.


EBV-specific humoral antibodies in nasopharyngeal carcinoma patients in Cuba.
Sera of 24 patients with nasopharyngeal carcinoma (NPC) and of 60 healthy donors were studied for presence of IgG and IgA antibodies against different antigens induced by Epstein--Barr virus (EBV). The results obtained demonstrate that NPC in Cuba, as in other countries, is accompanied by elevated antibody titers against EBV-specific IgG antibodies, whose levels strongly differ from those of healthy persons. Patients with NPC in Cuba had higher titers of antibody against VCA of EBV in comparison with patients of Caucasian origin in those countries where NPC, the same as in cuba, is rarely found. Cuban NPC patients had high levels of antibody against EA, often higher than in NPC patients in endemic zones of this disease.