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Biology, Ecology, and Control of Tick Vectors and Vertebrate Hosts of Rickettsial Pathogens
William L. Nicholson, PhD (Disease Ecology Laboratory) and Michael L. Levin, PhD (Medical Entomology Laboratory), Pathogen Biology and Ecology Activity, Rickettsial Zoonoses Branch.
The rickettsial diseases of humans are caused by a number of species in the genera Anaplasma, Ehrlichia, Orientia, Rickettsia, Neorickettsia, and Neoehrlichia. The ecologies of many rickettsial pathogens have not been elucidated, especially for many of the newly identified organisms. Natural cycles involve a complex interaction among the vector ticks, their vertebrate hosts, and the pathogens. A combination of studies utilizing fieldwork and supported by controlled laboratory experiments can provide valuable new information regarding these maintenance cycles and transmission dynamics. The ecological approach also identifies points of potential intervention to prevent or control the perpetuation of these cycles, and thus lead to reduced human exposure to these agents. Because we study many aspects of the pathogen life cycle, candidates with diverse backgrounds in entomology, wildlife biology, molecular biology, and immunology are strongly encouraged to apply. Projects can incorporate laboratory, field activities, or a combination of both. Potential project topics include: 1) field investigations to determine how enzootic cycles of transmission are maintained; 2) habitat suitability assessments to identify areas of greatest risk for tick and pathogen contact; 3) field or laboratory studies to better understand transmission dynamics in various tick-host systems; 4) laboratory investigations of the interaction of rickettsial co-infections; 5) evaluation of anti-tick and anti-pathogen vaccines; 6) vector or host competence studies; or 7) development and application of novel integrated tick control methods. Our two laboratories, unique tick vivarium, and additional branch resources provide the equipment and facilities for BSL-2/BSL-3 laboratory work, tick and animal studies, and pathogen detection using molecular, immunological, or microbiological methods.
- Prusinski, M. A., et al. 2014. Sylvatic epidemic typhus associated with flying squirrels (Glaucomys volans) in New York state, USA. Vector Borne Zoonotic Dis. 14 (4): 240-244.
- Pritt, B. S., et al. 2011. Emergence of a new pathogenic Ehrlichia species, Wisconsin and Minnesota, 2009. New Engl. J. Med. 365 (5): 422-429.
- Zemtsova, G., L. F. Killmaster, K. Y. Mumcuoglu, and M. L. Levin. 2010. Co-feeding as a route for transmission of Rickettsia conorii israelensis between Rhipicephalus sanguineus. Exp. Appl. Acarol. 52: 383-392.
- Zemtsova, G. E., et al. 2016. First report of Rickettsia identical to R. slovaca in colony-originated D. variabilis in the US: detection, laboratory animal model and vector competence of ticks. Vector Borne & Zoonotic Dis. 16: 77-84.
- Killmaster, L. F., et al. 2014. Detection of bacterial agents in Amblyomma americanum (Acari: Ixodidae) from Georgia, USA, and the use of a multiplex assay to differentiate Ehrlichia chaffeensis and Ehrlichia ewingii. J. Med. Entomol. 51:868-872.
Evaluation of the Standard Diagnostics Dengue duo Rapid Diagnostic Test
Tyler Sharp, PhD, Dengue Branch, email: firstname.lastname@example.com The Dengue Branch Epidemiology Activity solicits a Post-Doctoral Preceptor Candidate to work on projects based on the Sentinel Enhanced Dengue Surveillance System (SEDSS). The primary project would be a prospective evaluation of the Standard Diagnostics DengueDuo rapid diagnostic test (RDT) to determine the performance and clinical utility of the RDT. Responsibilities of the Candidate will be to develop study materials, train hospital and SEDSS staff on enrollment of patients into the nested study, integrate data collection tools into the pre-existing SEDSS database, and analyze study findings. Once the protocol is developed and the staff are trained, patient enrollment will begin once dengue case detection is high, since evaluating the test during non-epidemic levels of dengue case detection is will be unlikely to produce a sufficient number of dengue patients to enable appropriate evaluation of the RDT. Therefore, during this potential period of delay, a second project for the Candidate would be to analyze pre-existing data from the first 3 years of SEDSS (May 2012–April 2015) for characteristics (e.g., signs/symptoms, diagnoses, age groups, time of enrollment) associated with patients enrolled in SEDSS that did not have a pathogen identified by diagnostic testing. This will improve our understanding of the potential etiologic agents in patients enrolled in SEDSS as well as the utility of the current diagnostic algorithm, since such an analysis may suggest other causes of illness for which diagnostic testing is not routinely performed (e.g., acute gastroenteritis, localizing source of bacterial infection) or patients with false-negative test results under in the diagnostic algorithm. Last, depending on the Candidate's availability, a potential third project would be a comparative analysis of the incidence and clinical characteristics of dengue patients with viral encephalitis that were identified by the Passive Dengue Surveillance System (PDSS) versus those identified by SEDSS.
Molecular Mechanisms of Pathogenesis of Borrelia burgdorferi, the Lyme disease agent
Robert D. Gilmore, PhD, Bacterial Diseases Branch, email: email@example.com Lyme borreliosis, aka Lyme disease, continues to be a major public health concern in the USA, North America, and worldwide. A recent report by our CDC Division has estimated that there may be up to 300,000 cases of this tick transmitted illness diagnosed in this country. Understanding the biological processes of the causative agent, the spirochete Borrelia burgdorferi, is essential to be able to develop new strategies to combat this disease through prevention and control measures. My laboratory focuses on the pathogenesis of the Lyme disease agent utilizing molecular microbiological and immunological methodologies to study bacterial mechanisms of infection, e.g. pathogen transmission and survival at the tick/host interface. The objective is to identify B. burgdorferi genes that are differentially expressed during the enzootic cycle and to study the role of the encoded gene products in pathogenesis including putative protein‐protein interactions that mediate survival and infection of the spirochete in the tick and mammalian hosts. Our recent successes in identifying such B. burgdorferi genes promises fertile ground for research in understanding the role of these gene
products in vector‐host interactions, and is the basis for improving diagnostic assays, and developing new therapeutic strategies for Lyme disease.
Understanding the mechanisms of virus – vector specificity using chimeric alphaviruses.
Ann Powers, PhD, Arbovirual Diseases Branch, email: firstname.lastname@example.com Large and ongoing outbreaks of chikungunya virus (CHIKV) have caused greater than 4 million human infections around the globe in the past decade. The most closely related alphavirus, o'nyong nyong (ONNV), has caused only 2 documented but large scale outbreaks in East Africa. Although these viruses are genetically similar, they exhibit distinct ecologies and very different infection patterns of mosquito vector infection. Humans are infected with ONNV when bitten by infected Anopheles gambiae and An. funestus, while the mosquito vectors that transmit CHIKV are Aedes aegypti and Ae. albopictus. ONNV is the only alphavirus to be vectored by anopheline mosquitoes and studies have indicated that An. gambiae is highly susceptible to ONNV infection while it is refractory to CHIKV infection and that the nonstructural protein 3 (nsP3) of ONNV is largely responsible for its unique ability to infect An. gambiae. Recent work from the Alphavirus Laboratory has used chimeric viruses to study the infection patterns through artificial infectious feeds in An. gambiae and has demonstrated that ONNV nsP3 was instrumental in regulating infectivity of An. gambiae mosquitoes. Follow up studies to this publication need to focus on understanding the mechanisms of this process. By studying the virus-vector interactions of ONNV and CHIKV, we can not only shed light on the ability of these viruses to infect completely disparate vector hosts but also understand the exact mechanisms and functions of nsP3, a highly-variable protein whose properties with regard to viral pathogenesis are yet to be understood. This could lead to critical control strategies to prevent mosquitoes from becoming infected with human arboviruses.
Powers AM, Brault AC, Tesh RB, Weaver SC. 2000. Re-emergence of chikungunya and o'nyong-nyong viruses: evidence for distinct geographical lineages and distant evolutionary relationships.
J Gen Virol. 81: 471–479. Vanlandingham DL, Hong C, Klingler K, Tsetsarkin K, McElroy KL. 2005. Differential infectivities of o'nyong-nyong and chikungunya virus isolates in Anopheles gambiae and Aedes aegypti mosquitoes. Am J Trop Med Hyg. 72: 616–621. Saxton-Shaw KD, Ledermann JP, Borland EM, Stovall JL, Mossel EC. 2013. O'nyong nyong Virus Molecular Determinants of Unique Vector Specificity Reside in Non-Structural Protein 3. PLoS Negl Trop Dis 7(1): e1931. Myles KM, Kelly CL, Ledermann JP, Powers AM. Effects of an opal termination codon preceding the nsP4 gene sequence in the o'nyong-nyong virus genome on Anopheles gambiae infectivity. J Virol. 2006 May;80(10):4992-7.
Ecology of Mosquito Vectors and Transmission Dynamics of Arboviruses
H. M. Savage, PhD, Ecology, Entomology Activity, Arbovirus Diseases Branch, email: email@example.com Knowledge of the morphological and molecular identification, population structure, behavior and biology of mosquito vectors is essential to understanding the ecology and transmission dynamics of arboviral diseases and the development of vector control strategies. Research projects that integrate field, laboratory and molecular studies to address unknown or poorly understood aspects of mosquito biology and arbovirus transmission are encouraged. Projects should focus on Culex or Aedes mosquito species that are important vectors of arboviruses.
Savage, H.M., J.P. Ledermann, L. Yug, K.L. Burkhalter, M. Marfel and W.T. Hancock. 2015. Incrimination of Aedes (Stegomyia) hensilli Farner as an epidemic vector of chikungunya virus on Yap Island, Federated States of Micronesia, 2013. American Journal of Tropical Medicine and Hygiene 92:429-436. Kothera, L., B. M. Nelms, W.K. Reisen, and H.M. Savage. 2013. Population genetic and admixture analyses of Culex pipiens complex (Diptera: Culicidae) populations in California, United States. American Journal of Tropical Medicine and Hygiene 89:1154-1167. Kothera, L., M. Godsey, J.-P, Mutebi and H.M. Savage. 2010. A comparison of above ground and belowground populations of Culex pipiens (Diptera: Culicidae) mosquitoes in Chicago, Illinois, and New York City, New York, using microsatellites. Journal of Medical Entomology 47:805-813. Kent, R.J., S. Deus, M. Williams, and H.M. Savage. 2010. Development of a multiplexed polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay to identify common members of the subgenera Culex (Culex) and Culex (Phenacomyia) in Guatemala. American Journal of Tropical Medicine and Hygiene 83:285-291. Savage, H.M., M. Anderson, E. Gordon, others. 2008. Host-seeking heights, hostseeking activity patterns, and West Nile virus infection rates for members of the Culex pipiens complex at different habitat types within the hybrid zone, Shelby County, TN, 2002 (Diptera: Culicidae). Journal of Medical Entomology 45:276-288.
Identification of the mechanisms for vertebrate host-restricted replication and mosquito interference of flaviviruses with a mosquito-restricted host range
Aaron Brault, PhD, Arboviral Diseases Branch, email: firstname.lastname@example.com Nhumirim virus (NHUV) represents an example of a unique subset of apparently insectspecific viruses that phylogenetically affiliate with dual-host mosquito-borne flaviviruses(1). In vitro co-infection experiments have indicated that prior or concurrent infection of mosquito cells with NHUV resulted in a 10,000-fold reduction in West Nile virus (WNV) titers(2). Additionally, co-intrathoracic inoculation of Culex quinquefasciatus demonstrated significantly lower transmission rates compared to mosquitoes inoculated with WNV alone(3). These data indicate the potential that infection of Culex spp. vectors with NHUV could serve as a barrier for efficient transmissibility of flaviviruses associated with human morbidity/mortality; however, no data exists pertaining to the mechanism(s) of in vitro or in vivo superinfection exclusion (SIE). Furthermore, there is a paucity of data on the viral genetic determinants or host cellular pathways that restrict host range for flaviviruses. The phylogenetic placement of NHUV among dual host flaviviruses and a codon usage of NHUV that resembles that of flaviviruses that circulate between both vertebrate and mosquito hosts indicates the likelihood that this group of viruses has only recently lost its capacity to replicate in vertebrate cells(2, 4). As such, this affords an excellent model for dissecting both viral and host factors relevant to such a host
restrictive phenotype. The identification of the viral genetic determinants of vertebrate replication and host restriction pathways could be interrogated by assessing the vertebrate infection phenotype of NHUV/WNV chimeric viruses and by screening vertebrate cell knockdown libraries, respectively. There is a potential for SIE to alter the natural transmissibility of flaviviruses and for potential use in the derivation of novel methodologies for reducing transmission of flavivirus. As such, the study of SIE mechanism(s) is proposed be elucidated through the use of small RNA libraries, intracellular trafficking and/or exogenous expression of decoy RNAs.
1. Pauvolid-Correa A, Solberg O, Couto-Lima D, Kenney J, Serra-Freire N, Brault A, Nogueira R, Langevin S, Komar N. 2015. Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil. Arch Virol 160:21-27. 2. Kenney JL, Solberg OD, Langevin SA, Brault AC. 2014. Characterization of a novel insectspecific flavivirus from Brazil: potential for inhibition of infection of arthropod cells with medically important flaviviruses. J Gen Virol 95:2796-2808. 3. Goenaga S, Kenney JL, Duggal NK, Levis SC, Enria DA, Brault AC. 2015. Previous infection with Nhumirim virus (Flaviviridae; Flavivirus) inhibits efficient transmission of West Nile virus by Culex quinquefasciatus mosquitoes Viruses in press. 4. Blitvich B, Firth A. 2015. Insect-Specific Flaviviruses: A Systematic Review of Their Discovery, Host Range, Mode of Transmission, Superinfection Exclusion Potential and Genomic Organization. Viruses 7:1927-1959.
Advanced Public Health Laboratory Studies on Rickettsial Diseases and their Vectors
Gregory A. Dasch, Ph.D., Rickettsial Zoonoses Branch, email: email@example.com The Biotechnology Applications Laboratory (BAL) of the Rickettsial Zoonoses Branch conducts both field and laboratory studies using next generation sequencing and proteomics to improve the diagnosis, prevention, control and treatment of rickettsial vector‐borne diseases. Strong emphasis is placed on the most severe and/or fatal tick borne‐disease in the Americas, Rocky Mountain spotted fever, caused by Rickettsii rickettsii and the Select Agent Rickettsia prowazekii. R. rickettsii also serves as the primary laboratory model for investigations with other Rickettsia agents causing human rickettsioses of varying clinical presentation caused by an ever expanding list of emerging organisms and the vectors, largely ticks, which transmit rickettsioses. Relatively little is known about the molecular mechanisms used by obligately intracellular bacteria to subvert host defense mechanisms in either humans or their arthropod vectors. The BAL laboratory is applying whole genome, transcriptomics, cellular biology, and proteomics approaches to Rickettsia to better understand their surface antigen diversity, pathogenic effector mechanisms, and specific host adaptations. Investigations on the physiology of tick vectors, their genomics, and their total bacterial community are also being conducted. Depending on the research interests of individual candidates, targeted research proposals are solicited including (1) molecular epidemiology/genomics of Rickettsia, and discovery of clinical biomarkers of rickettsial diseases; (2) dissection of vector‐bacterial community‐host interactions (disease ecology); (3) proteomic and metabolomics investigations of rickettsial alterations of host cell physiology; (4) cell biology, immunology and pathologic responses of vertebrate and invertebrate cells to rickettsial infections; and (5) development of
medical countermeasures (vaccines, assays, new therapies) for treating, diagnosing or preventing rickettsial diseases. While work with live Rickettsia must be performed in a Biosafety Level 3 laboratory, only such work with R. prowazekii requires Select Agent approvals. Work with animals and vectors and field studies require IACUC approval and animal training as well as field collection permits.
Bonilla, D. L.., L. A. Durden, M. E. Eremeeva, and G. A. Dasch. 2013. The biology and taxonomy of head and body lice — Implications for louse borne disease prevention. PLOs Pathogens 9(11): e1003724. Prusinski, M. A., J. L. White, S. J. Wong, others. 2014. Sylvatic typhus associated with flying squirrels (Glaucomys volans) in New York State, United States. Vector-Borne Zoon Dis. Vector Borne Zoonotic Dis. 14(4):240-244. Williams‐Newkirk, A. J., L. A. Rowe, T. R. Mixson‐Hayden, and G. A. Dasch. 2014. Characterization of the bacterial communities of life stages of free living lone star ticks (Amblyomma americanum). PLoS One 9(7):e102130. doi: 10.1371/journal.pone.0102130. Eremeeva, M. E., and G. A. Dasch. 2015. Challenges posed by tick-borne rickettsiae: eco-epidemiology and public health implications. Frontiers Publ Hlth 3(15):1-17. Williams-Newkirk AJ, Burroughs M, Changayil SS, Dasch GA. 2015. The mitochondrial genome of the lone star tick (Amblyomma americanum). Ticks Tick Borne Dis. 6(6):793-801.
Ecology and Control of Ticks and Lyme disease spirochetes
Rebecca J. Eisen, PhD, and Lars Eisen, Ph.D. Ecology, Entomology Activity (EEA), Bacterial Diseases Branch, email: firstname.lastname@example.com
Lyme disease, caused by Borrelia burgdorferi sensu lato (Bbsl) is the most commonly reported vectorborne disease in the United States. Since the late 1990s, the number of reported cases has tripled and the primary tick vector (Ixodes scapularis) has continued to expand its geographical range. The primary objectives of our laboratories are to 1) Determine when and where humans are at greatest risk for exposure to ticks and Lyme disease spirochetes; 2) Improve understanding of how Lyme disease spirochetes are maintained in enzootic transmission cycles, and 3) Mitigate risk of exposure to infected ticks through improved surveillance and management strategies. Applied research projects that integrate field, laboratory, molecular or modeling studies to address these broad objectives are encouraged. Examples might include: 1) development of improved pathogen detection assays for ticks and tissues from mammalian reservoirs, 2) field studies aimed at identifying where humans are most at risk for exposure to infected ticks, 3) field and laboratory studies to elucidate how Bbsl spirochetes are maintained in enzootic transmission cycles, and 4) development and evaluation of personal protection measures against ticks (e.g., repellents, permethrin-treated clothing) or environmentally based tick/control methods (chemical and biological agents to suppress host-seeking ticks or tick host-targeted control methods).
Eisen, R.J., L. Eisen and C.B Beard. 2016. County-scale distribution of Ixodes scapularis and Ixodes pacificus (Acari: Ixodidae) in the continental United States, Journal of Medical Entomology, in press.
Eisen, R.J., L. Eisen, N.H. Ogden, and C.B. Beard. 2016. Linkages of weather and climate with Ixodes scapularis and Ixodes pacificus (Acari: Ixodidae), enzootic transmission of Borrelia burgdorferi, and Lyme disease in North America. 2016. Journal of Medical Entomology, in press.
Johnson, T.L., J. Bjork, D. Neitzel, F. Dorr, E. Schiffman, and R.J. Eisen*. 2016. Habitat suitability model for the distribution of black-legged ticks, Ixodes scapularis (Acari: Ixodidae), in Minnesota, Journal of Medical Entomology, in press. Springer, Y.P., C.S. Jarnevich, D.T. Barnett, A.J. Monaghan, and R.J. Eisen*. 2015. Modeling the present and future distribution of the lone star tick, Amblyomma americanum (Ixodida: Ixodidae), in the continental United States, American Journal of Tropical Medicine and Hygiene, 93: 875-890. Monaghan, A.J., S.M. Moore, K.M. Sampson, C.B. Beard, and R.J. Eisen*. 2015. Climate change influences on the annual onset of Lyme disease in the United States, Ticks and Tick-Borne Diseases, 6: 615-622. Newman, E.A., L. Eisen, R.J. Eisen, N. Fedorova, J.M. Hasty, C.E. Vaughn, and R.S. Lane. 2015. Borrelia burgdorferi sensu lato spirochetes in wild birds in northwestern California: associations with ecological factors, bird behavior and tick infestation." PLoS One PLoS One 10(2): e0118146. Moore, S.M., R.J. Eisen, A. Monaghan, and P. Mead. 2014. Meteorological influences on the seasonality of Lyme disease in the United States, American Journal of Tropical Medicine and Hygiene, 90: 486-496. Eisen, R.J., J. Piesman, E. Zielinski-Gutierrez, and L. Eisen. 2012. What do we need to know about disease ecology to prevent Lyme disease in the Northeastern United States? Journal of Medical Entomology 49: 11-22
Q Fever: Improved therapy for C. burnetii infections
Gilbert J. Kersh, PhD, Rickettsial Zoonoses Branch, email: firstname.lastname@example.com Coxiella burnetii is an obligate intracellular bacterium, a category B bioterrorism agent, and the etiologic agent of Q fever. Q fever is transmitted by inhalation of the organisms usually derived from waste products of infected animals and results in an acute febrile illness characterized by fever headache, and in some cases pneumonia. In a minority of cases, a chronic infection develops which can be life-threatening and typically manifests as culture negative endocarditis. Treatment for acute Q fever is a 2-week course of the antibiotic doxycycline, whereas chronic Q fever requires 18 months of treatment with doxycycline plus hydroxychloroquine. Alternative therapies are needed due to the difficulty of compliance over such a long treatment course and the frequency with which patients are unable to tolerate these drugs. In previous work, we have established in vitro assays for drug efficacy that include analysis in cultured cell lines as well as liquid suspension culture. Research opportunities are available which include (i) Evaluation of the pH dependence of C. burnetii growth and antibiotic function; (ii) Testing of novel drug combinations for long-term treatment (iii) Evaluation of immune responses as means to eliminate C. burnetii; (iv) In vivo testing of alternative treatments in a mouse model of C. burnetii aerosol infection.
Studies into the pathogenesis of Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever
Sandor E. Karpathy, PhD and Chris Paddock, MD, MPHTM, Rickettsial Zoonoses Branch, email: email@example.com Rickettsia rickettsii is the most pathogenic of all known rickettsial species and continues to cause a substantial number of human cases of Rocky Mountain spotted fever (RMSF) throughout the Western Hemisphere. While the case fatality rate of RMSF has fallen dramatically with the introduction of antibiotics, early treatment is still essential as delayed
treatment leads to severe disease and mortality. Additionally, case fatality rates in Latin America are 3-5x higher than in the US, and in some countries approach 40%. Little is known about the pathogenic mechanisms involved in R. rickettsii infection. Recent work in our lab has shown that genetic differences exist between various strains of R. rickettsii that directly correlate with their geographical origins; however, it is unknown if this differentiation also represents differences in the relative virulence of the isolates. An increased understanding of the pathogenic mechanisms and regional differences in case-fatality rates of R. rickettsii, and the variation in virulence among different isolates, is needed to better assess the threat of human disease. Research opportunities are available to 1) investigate the relative virulence of geographically distinct isolates of R. rickettsii utilizing cell culture and animal models 2) investigate host cellular response to rickettsial infection, and 3) investigate rickettsial gene expression during infection in vivo and in vitro.
Karpathy SE, Dasch GA, Eremeeva ME. 2007. Molecular Typing of Isolates of Rickettsia rickettsii by Use of DNA Sequencing of Variable Intergenic Regions. J Clin Microbiol 45:25452553. Paddock CD, Denison AM, Lash RR, Liu L, others. 2014. Phylogeography of Rickettsia rickettsii genotypes associated with fatal Rocky Mountain spotted fever. Am J Trop Med Hyg 91:589-597. Labruna MB, Santos FC, Ogrzewalska M, others. 2014. Genetic identification of rickettsial isolates from fatal cases of Brazilian spotted fever and comparison with Rickettsia rickettsii isolates from the American continents. J Clin Micro 53: 3788-3791. Alvarez-Herandez G, Murillo-Benitez C, Candia-Plata, M, Moro, M. 2015. Clinical profile and predictors of fatal Rocky Mountain spotted fever in children from Sonora, Mexico. Pediatr Infect Dis J 34:125-130. Angerami RN, Camara M, Pacola MR, others. 2012. Features of Brazilian spotted fever in two different endemic areas in Brazil. Ticks Tick Borne Dis 3:346-348.
Deadline: April 30, 2016
Program The Division of Vector-Borne Diseases (DVBD) is part of the National Center for Emerging and Zoonotic Infectious Diseases of the Centers for Disease Control and Prevention (CDC). The DVBD conducts a wide range of investigations aimed at finding and implementing better methods to detect, prevent and control viral and bacterial pathogens transmitted by blood-feeding arthropods. The 90 Division doctoral staff include ecologists, epidemiologists, laboratorians, molecular biologists, virologists, bacteriologists, zoologists, entomologists, statisticians, and modellers, as well as a professional technical staff.
The DVBD comprises four branches: Arboviral Diseases Branch, Fort Collins, Colorado – o West Nile, Zika, yellow fever, Heartland, chikungunya, all arboviruses Bacterial Diseases Branch, Fort Collins, Colorado – o Lyme disease, plague, tularaemia, tick-borne relapsing fever, etc. Dengue Branch, San Juan, Puerto Rico – dengue and other endemic arboviruses Rickettsial Zoonoses Branch, Atlanta, Georgia – o Rocky Mountain spotted fever, Ehrlichia, Anaplasma, Coxiella More can be read about the DVBD, its work, and accomplishments at: http://www.cdc.gov/ncezid/dvbd/.
The purpose of the fellowship program is to provide opportunities for recently graduated scientists to gain experience at the forefront of vector-borne disease research. Equally important it is a means for DVBD staff to benefit from the fellows' fresh skills and perspective.
Funding The Fellowship is a temporary Federal appointment at an annual salary of $63,691 – $70,060, depending on qualifications, for a maximum of 2 years.
Eligibility Applicants should have completed a doctoral degree (PhD, MD, ScD, DVM, or equivalent) or present evidence they have completed all requirements for a degree pending award of diploma by the fellowship start date. This opportunity is not open to staff or fellows currently at DVBD. Applicants should also have earned their doctorate degree or completed a primary residency within four years of their proposed start date. Applicants may not have a faculty position or be enrolled in a graduate degree program during the fellowship. Review of applications and selection will be made without regard to race, creed, color, age, sex, or national origin. Non-US citizens or permanent residents must provide evidence of a valid J-1 visa prior to starting tenure at the CDC.
Pre-Application Requirements Collaboration with a Preceptor: Before initiating an application, identify a research project and preceptor listed in the Opportunities for Research and Principal Scientist Listing (http://www.cdc.gov/ncezid/dvbd/). As soon as possible, contact the preceptor to discuss your experience and possible research topics. If encouraged to do so, develop a brief research plan. Collaboration is required before submitting your application, and must be verified by the preceptor after submission. Applications submitted without preceptor collaboration will be disqualified.
Application Package Applications must be received by April 30, 2016. The following items should be included: Curriculum Vitae that includes name, contact information, summary of education and work experience with dates, and professional accomplishments, such as publications and presentations. Include a brief statement of your research interests, technical strengths and professional goals.
Research Proposal (less than 2 pages) that includes title of project, name of preceptor, objective, methods to be employed, and future steps to be taken if the objective is reached and if it is not. Letters of Recommendation (minimum 3) should be received at the application mailbox (below) by April 30, 2016. Letters should include the recommenders' contact information. Please include the applicants name in the subject line of the recommendation.
Criteria for Selection Applications are reviewed for (i) scientific merit and training potential of the research proposal, (ii) training resources and environment from the identified preceptor, and (iii) congruence with the Centers for Diseases public health mission.
Notification Process for 2016 Applicants April 30, 2016 – Application deadline Mid May, 2016 – Notification for Ineligible and Disqualified Applicants Mid-June, 2016 – Award notifications October-December, 2016 – Start date (negotiable)
Applications, Letters of Recommendation, and Questions E-mail: firstname.lastname@example.com Telephone: (970) 221-6400


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Welcome to our fifth issue of North East Living magazine.
After one of the coldest winters on record in many of our regional centres, Spring has finally arrived, bringing with it renewed energy and excitement about the warmer weather to come. It's the season to be inspired, embark on new projects and spend more time in the great outdoors exploring all that's on offer in the beautiful North East. The region is blessed with some of the most pristine and spectacular waterways in the country, which not only attract hikers and fishers, but members of the North East Canoe Club who are well prepared to tackle the challenging white water rapids in our local rivers. Spring produce is also making its presence felt on local menus where chefs including Wodonga's David Kapay from Miss Amelie, and Rutherglen's Simon Arkless from All Saints Estate Terrace Restaurant, are letting the finest ingredients speak for themselves. This month we hear from David Kapay about his amazing experience in two of the world's best restaurants where he honed his skills under kitchen titans Jamie Oliver, Gordon Ramsay and Jason Atherton. We also head to Myrrhee where walnut producers Mike Burston and Carol Kunert know more than most about the challenges of our extreme seasons, while the history of the Glenrowan region is brought to life through the memories of local legend Linton Briggs. We hope this issue inspires you to get out and about, see the region with fresh eyes and make the most of the many jewels to be found right on our doorstep.

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Search results for "Meuschenia freycineti"
Results - 1 to 8 of 8
Sixspine Leatherjacket, Meuschenia freycineti (Quoy & Gaimard, 1824)
The colouration of Sixspine Leatherjackets change with growth. The species is endemic to Australia.
Sixspine Leatherjacket, Meuschenia freycineti
Monacanthidae - Leatherjackets
Use the table to access images and fact sheets of the monacanthid fishes on the site.
Young male Sixspine Leatherjacket
Sixspine Leatherjacket at Montague Island
This week in Fish: First Kidako Moray image
This week sees the addition of some great new movies. The Halfband Snake Eel descending backwards into the sand is pretty amazing. The Lepidion swimming at over a kilometre below the surface is worth a look and the male Pacific Leaping Blenny 'frantically' trying to attract females certainly brought a smile to my face. There are also some great new images. What is possibly the first photo of a live Kidako Moray and the oh-so-cute baby boxfish are just two of a great bunch. Thank you to everyone who contributed.
Threadfin Leatherjackets schooling
Fishes of Sydney Harbour
Sydney Harbour has a very rich fish fauna. Nearly 600 species live in it, enter it, or are known from historic records.
Results - 1 to 8 of 8


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In the course of the visit of Jens Stoltenberg will visit military bases and the plant for the production of fighter
NATO Secretary General Jens Stoltenberg this week will arrive in the United States, where he will visit a factory for the production of fighters and command centers of the us armed forces.
On Thursday, the NATO chief will go to the Lockheed Martin plant in Texas, which produces the F-35, and will also participate in a public event at southern Methodist University, where he will answer students ' questions.
On Friday Stoltenberg will visit Sheppard air force base 80th training wing flight training in Texas, will meet with the participants of the joint training of pilots of Europe and NATO, and later with representatives of the Strategic command at the airbase of Offutt in Nebraska.
He will also visit the Command of aerospace defense of the North American continent and Northern command on Peterson air force base in Colorado.


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Custom Excel Formula
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Farmhouse Table & Bench ( Country Kitchen Table With Bench Idea #2)
Home » Bench » Country Kitchen Table With Bench » Farmhouse Table & Bench ( Country Kitchen Table With Bench Idea #2)
Farmhousefarm•house (färm′hous′),USA pronunciation n., pl. -hous•es
(-hou′ziz).USA pronunciation
- a house on a farm, esp. the one used by the farmer and farmer's family.
Tableta•ble (tā′bəl),USA pronunciation n., v., -bled, -bling, adj.
- an article of furniture consisting of a flat, slablike top supported on one or more legs or other supports: a kitchen table; an operating table; a pool table.
- such a piece of furniture specifically used for serving food to those seated at it.
- the food placed on a table to be eaten: She sets a good table.
- a group of persons at a table, as for a meal, game, or business transaction.
- a gaming table.
- a flat or plane surface;
a level area.
- a tableland or plateau.
- a concise list or guide: a table of contents.
- an arrangement of words, numbers, or signs, or combinations of them, as in parallel columns, to exhibit a set of facts or relations in a definite, compact, and comprehensive form;
a synopsis or scheme.
- (cap.) the constellation Mensa.
- a flat and relatively thin piece of wood, stone, metal, or other hard substance, esp. one artificially shaped for a particular purpose.
- a course or band, esp. of masonry, having a distinctive form or position.
- a distinctively treated surface on a wall.
- a smooth, flat board or slab on which inscriptions may be put.
- tables:
- the tablets on which certain collections of laws were anciently inscribed: the tables of the Decalogue.
- the laws themselves.
- the inner or outer hard layer or any of the flat bones of the skull.
- a sounding board.
- [Jewelry.]
- the upper horizontal surface of a faceted gem.
- a gem with such a surface.
- on the table, [Parl. Proc.]
- [U.S.]postponed.
- [Brit.]submitted for consideration.
- turn the tables, to cause a reversal of an existing situation, esp. with regard to gaining the upper hand over a competitor, rival, antagonist, etc.: Fortune turned the tables and we won. We turned the tables on them and undersold them by 50 percent.
- under the table:
- drunk.
- as a bribe;
secretly: She gave money under the table to get the apartment.
- wait (on) table, to work as a waiter or waitress: He worked his way through college by waiting table.Also, wait tables.
- to place (a card, money, etc.) on a table.
- to enter in or form into a table or list.
- [Parl. Proc.]
- [Chiefly U.S.]to lay aside (a proposal, resolution, etc.) for future discussion, usually with a view to postponing or shelving the matter indefinitely.
- to present (a proposal, resolution, etc.) for discussion.
ta′ble•less, adj.
- of, pertaining to, or for use on a table: a table lamp.
- suitable for serving at a table or for eating or drinking: table grapes.
Benchbench (bench),USA pronunciation n.
- a long seat for several persons: a bench in the park.
- a seat occupied by an official, esp. a judge.
- such a seat as a symbol of the office and dignity of an individual judge or the judiciary.
- the office or dignity of various other officials, or the officials themselves.
- the seat on which the players of a team sit during a game while not playing.
- thequality and number of the players of a team who are usually used as substitutes: A weak bench hurt their chances for the championship.
- [Informal.]See bench press.
- Also called workbench. the strong worktable of a carpenter or other mechanic.
- a platform on which animals are placed for exhibition, esp. at a dog show.
- a contest or exhibition of dogs;
dog show.
- [Phys. Geog.]a shelflike area of rock with steep slopes above and below.
- a step or working elevation in a mine.
- berm (def. 2).
- on the bench:
- serving as a judge in a court of law;
- [Sports.](of a player) not participating in play, either for part or all of a game.
bench′less, adj.
- to furnish with benches.
- to seat on a bench or on the bench: an election that benched him in the district court.
- to place (a show dog or other animal) in exhibition.
- to cut away the working faces of (a mine or quarry) in benches.
- to remove from a game or keep from participating in a game: to be benched because of poor hitting.
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Farmhouse Table & Bench ( Country Kitchen Table With Bench Idea #2) design has turned into a favored type of many individuals to their property. The style is elegant, easy and modern glance has fascinated many people to use for their occupancy. Getting a contemporary modern look gorgeous? for modern design type has an appealing trait, the furniture is designed.
The style style furnishings provide the effect of basic and sunshine inside the room's closing appearance. This is often purchased by the use of a smooth line that was straight to make use of white colour thus satisfied lighting and clear. Another product applied is glass content that is reflective and clear to give the more modern's impression.
Flooring with products such as ceramics timber, porcelain tile effectively joined inside the contemporary category. Offer to freeze area aesthetically also concluding rather like a rug for one more impression of luxury. This strategy is for distancing between the family area which usually appear next to each other along with the dining area, most well suited.
Today with modern modern interior design, room is created open and vibrant with sun light in the place. So that light can be replicated around the bedroom in the home pick white floor material. Additionally utilize glass rather than large windows wall substance and skylights to bring in light that is day around feasible internal.
Farmhouse Table & Bench ( Country Kitchen Table With Bench Idea #2) design style's color palette is centered from the scheme of neutral hues like black, brown, dreary, and white. Employ these shades for interior elements including walls, ground, threshold, and arranging a spot to get a splash of vivid colors in components and furniture of the space.
Use your imagination for a more innovative process styles and designs to supply a splendor that is striking inside the room. Opportunities have exposed for the material used to accomplish out interior design stand is. The impact that's felt in contemporary interior planning is collections that are minimum and setting " less material ".
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Job Opportunities in Pak Austria Fachhochschule, Institute Of Applied Sciences. Positions: Environmental Process & Energy Engineering Digital Media Technologies Last Date: 06 Feb 2018Read More »
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Job Opportunities in Azad Jammu And Kashmir Information Technology Board. Positions: System Engineer Network Administrator System Engineer Web Programmer Project Manager IT Computer Programmer Web Developer Project Manager Last Date: 31 Jan 2018Read More »
Book of the Month – "Bohat Kamayen"
Engineering Cap has started the segment of introducing books that are worth reading for professional career and business. This would be a regular feature in Engineering Cap magazine. The title of the book for December is "Bohat Kamayen" (Earn a lot). The book is in Urdu language and written by …Read More »
Jobs In Headquarter National Logistics Cell 2018
Jobs In Headquarter National Logistics Cell 2018. National Logistics Cell has announced various jobs / career opportunities for mega construction project at Landikotal / Torkam. Available vacancies are Senior Project Manager, Deputy Project Manager, Senior Manager Contract, Manager Contract, Senior Manager/Manager Monitoring, Senior Manager Planning, Manager Planning, Senior Manager Procurement, Manager …Read More »
Counter Point
Counter Point by Engr. Asghar Hayat According to administration of Pakistan Engineering Council (PEC) and the official announcements through website and social media, the Prime Minister of Pakistan, Engr. Shahid Khaqan Abbasi has approved the demand of service structure for engineers during the Engineers Convention 2017. However, people and stakeholders …Read More »
Service Structure Paid Internships for Engineers Approved
Service Structure Paid Internships for Engineers Approved. Report by: Sarmad Shahbaz & Jayaa Jaggi On Thursday 9th November 2017, the most awaited mega-event Engineers Convention 2017 was held at the Jinnah Convention Center, Islamabad. This tremendous convention was organized by the Pakistan Engineering Council (PEC) for the Young and professional Engineers. …Read More »
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Schneider Electric is leading the Digital Transformation of Energy Management and Automation in Homes, Buildings, Data Centers, Infrastructure and Industries. With global presence in over 100 countries, Schneider is the undisputable leader in Power Management – Medium Voltage, Low Voltage and Secure Power, and in Automation Systems. We provide integrated efficiency …Read More »
How Prime Minister Envisions Engineering in Pakistan
Prime Minister of Pakistan Mr. Shahid Khaqan Abbasi, who himself is an Electrical Engineer, while speaking here at the Engineers Convention said that engineers were main to evolution and improvement of the country. He stated that "It is a mere coincidence that several ministers including that of the interior, defense, planning, …Read More »


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As you are advocates of reform in every thing; susceptible of reform, I wish to present a few remarks in advocacy of reform in weights and measures. It seems to me to be entirely unnecessary to have three or four dif ferent kinds of weights. I have found, by many years' experience in teaching, that it is very perplexing to students, and unnecessari ly retards their progress, in having to learn so many tables, and still more perplexing to go through the exercises under these tables.— But as I would not object to anything with out offering a substitute, I propose the follow ing :— Let Apothecaries and Troy weight be abo lished, and let us have such divisions of the lower denominations of Avoirdupois weight as may be necessary to express the smallest quantities desired. And where is the neces sity for so many kinds of measure? If all our measures of capacity have the same unit, why not have the same number of units for the same denomination in all the tables ? Let us have but one measure for all solids and li quids, and let our present standard of dry measure be made that standard. Our tables of long, square, and solid measure, I would not have altered. There is a vast deal of ig norance among the people on this subject, particularly in reference to measures. Many do not seem to know that Congress alone has power to establish weights and measures; and hence we hear of Tennessee measure, Al abama measure, &c. Such a State gives 32 quarts to the bushel, and another gives 40 quarts, &c. Now if a cubic inch is the mea suring unit, and the law requires a bushel to contain 21504 of these units, the value of a bushel will not be changed by dividing it into 32 parts, or into 32,000 parts; for the sum of the parts is equal to the whole. But it a quart is one thirty-second part of 2150'4 inches=67 2 inches, then no community has a right to set up a standard that requires 40 quarfcB, or any other nutrber oi quarts to the bushel, inasmuch as ifc would be an open vio lation of the constitution, Art 1, Sec. 8, Sub. Sec. 5. B. W. WHITE. Bear Spring Seminary, Giles Co., Tenn.
Weights and Measures
This article was originally published with the title "Weights and Measures"


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You love your work but the only track playing in the radio of your brain is
When is your business going to get traction? When are you FINALLY going to grasp this whole
marketing-your-business-is-easy mantra everyone is talking about?
I totally freaked out at even the tiniest bit of wastage from my children's dinner plates because I was so petrified about where the next contribution was coming from.
And I hated feeling like I was lying through my teeth when EVERYONE – especially the in laws insisted on asking me on a minute by minute basis how everything was going with the business. Fake smile plastered on face and 'fine' through gritted teeth, hoping that my husband hadn't already given the game away.
I knew that I needed to do something differently if I was going to succeed. But I wasn't sure what. Then I saw a marketing course being advertised that felt like was my last chance. I had to learn from people who had done it before and been successful… and so I took the plunge.
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I've since gone on to spend more than 10x that on developing my business and I don't regret a penny.
And there have been more than just a couple of days of late when you've wondered whether you should just give up.
You're so used to running around trying to fit everything in, the kids tea, the school run, the cleaning, the admin, the 'getting yourself out there,' but it's just not working, is it?
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- You're great at what you do, but you are not attracting enough clients… sometimes you even wonder if you should just give it all up
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- Anyone who is expecting a magic wand to "fix" their business
- Anyone who is unwilling to do the work needed to succeed
- Anyone who is not prepared to continue implementing the lessons once the course is finished


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The Killer by Stewart Edward White
Chapter XV
They had no tender feelings about me, however. Nobody cared whether I ever ate or not. I was led into the little ranch office and catechized to a fare-ye-well. They sat and roosted and squatted about, emitting solemn puffs of smoke and speaking never a word; and the sun went down in shafts of light through the murk, and the old shadows of former days crept from the corners. When I had finished my story it was dusk.
And on the heels of my recital came the sound of hoofs in a hurry; and presently loomed in the doorway the gigantic figure of Tom Thorne, the sheriff. He peered, seeing nothing through the smoke and the twilight; and the old timers sat tight and smoked.
"Buck Johnson here?" asked Thorne in his big voice.
"Here," replied the senor.
"I am told," said Thorne, directly, "that there is here an assembly for unlawful purposes. If so, I call on you in the name of the law to keep the peace."
"Tom," rejoined Buck Johnson, "I want you to make me your deputy."
"For what purpose?"
"There is a dispossession notice to be served hereabouts; a trespasser who must be put off from property that is not his."
"You men are after Hooper, and I know it. Now you can't run your neighbours' quarrels with a gun, not anymore. This is a country of law now."
"Tom," repeated Buck in a reasoning tone, "come in. Strike a light if you want to: and take a look around. There's a lot of your friends here. There's Jim Carson over in the corner, and Donald Macomber, and Marcus Malley, and Dan Watkins."
At this slow telling of the most prominent names in the southwest cattle industry Tom Thorne took a step into the room and lighted a match. The little flame, held high above his head, burned down to his fingers while he stared at the impassive faces surrounding him. Probably he had thought to interfere dutifully in a local affair of considerable seriousness; and there is no doubt that Tom Thorne was never afraid of his duty. But here was Arizona itself gathered for purposes of its own. He hardly noticed when the flame scorched his fingers.
"Tom," said Buck Johnson after a moment, "I heerd tell of a desperate criminal headed for Grant's Pass, and I figure you can just about catch up with him if you start right now and keep on riding. Only you'd better make me your deputy first. It'll sort of leave things in good legal responsible hands, as you can always easy point out if asked."
Tom gulped.
"Raise your right hand," he commanded, curtly, and administered the oath. "Now I leave it in your hands to preserve the peace," he concluded. "I call you all to witness."
"That's all right, Tom," said Buck, still in his crooning tones, taking the big sheriff by the elbow and gently propelling him toward the door, "now as to this yere criminal over toward Grant's Pass, he was a little bit of a runt about six foot three tall; heavy set, weight about a hundred and ten; light complected with black hair and eyes. You can't help but find him. Tom's a good sort," he observed, coming back, "but he's young. He don't realize yet that when things get real serious this sheriff foolishness just nat'rally bogs down. Now I reckon we'd better talk to the girl."
I made a beeline for the cook house while they did that and filled up for three. By the time I had finished, the conference was raised, and men were catching and saddling their mounts. I did not intend to get left out, you may be sure, so I rustled around and borrowed me a saddle and a horse, and was ready to start with the rest.
We jogged up the road in a rough sort of column, the old timers riding ahead in a group of their own. No injunction had been laid as to keeping quiet; nevertheless, conversation was sparse and low voiced. The men mostly rode in silence smoking their cigarettes. About half way the leaders summoned me, and I trotted up to join them.
They wanted to know about the situation of the ranch as I had observed it. I could not encourage them much. My recollection made of the place a thoroughly protected walled fortress, capable of resisting a considerable assault.
"Of course with this gang we could sail right over them," observed Buck, thoughtfully, "but we'd lose a considerable of men doing it."
"Ain't no chance of sneaking somebody inside?" suggested Watkins.
"Got to give Old Man Hooper credit for some sense," replied the senor, shortly.
"We can starve 'em out," suggested somebody.
"Unless I miss the old man a mile he's already got a messenger headed for the troops at Fort Huachuca," interposed Macomber. "He ain't fool enough to take chances on a local sheriff."
"You're tooting he ain't," approved Buck Johnson. "It's got to be quick work."
"Burn him out," said Watkins.
"It's the young lady's property," hesitated my boss. "I kind of hate to destroy it unless we have to."
At this moment the Morgan stallion, which I had not noticed before, was reined back to join our little group. Atop him rode the diminutive form of Artie Brower whom I had thought down and out. He had evidently had his evening's dose of hop and under the excitation of the first effect had joined the party. His derby hat was flattened down to his ears. Somehow it exasperated me.
"For heaven's sake why don't you get you a decent hat!" I muttered, but to myself. He was carrying that precious black bag.
"Blow a hole in his old walls!" he suggested, cheerfully. "That old fort was built against Injins. A man could sneak up in the shadow and set her off. It wouldn't take but a dash of soup to stick a hole you could ride through a-horseback."
"Soup?" echoed Buck.
"Nitroglycerine," explained Watkins, who had once been a miner.
"Oh, sure!" agreed Buck, sarcastically. "And where'd we get it?"
"I always carry a little with me just for emergencies," asserted Brower, calmly, and patted his black bag.
There was a sudden and unanimous edging away.
"For the love of Pete!" I cried. "Was there some of that stuff in there all the time I've been carrying it around?"
"It's packed good: it can't go off," Artie reassured us. "I know my biz."
"What in God's name do you want such stuff for!" cried Judson.
"Oh, just emergencies," answered Brower, vaguely, but I remembered his uncanny skill in opening the combination of the safe. Possibly that contract between Emory and Hooper had come into his hands through professional activities. However, that did not matter.
"I can make a drop of soup go farther than other men a pint," boasted Artie. "I'll show you: and I'll show that old----"
"You'll probably get shot," observed Buck, watching him closely.
"W'at t'hell," observed Artie with an airy gesture.
"It's the dope he takes," I told Johnson aside. "It only lasts about so long. Get him going before it dies on him."
"I see. Trot right along," Buck commanded.
Taking this as permission Brower clapped heels to the stallion and shot away like an arrow.
"Hold on! Stop! Oh, damn!" ejaculated the senor. "He'll gum the whole game!" He spurred forward in pursuit, realized the hopelessness of trying to catch the Morgan, and reined down again to a brisk travelling canter. We surmounted the long, slow rise this side of Hooper's in time to see a man stand out in the brush, evidently for the purpose of challenging the horseman. Artie paid him not the slightest attention, but swept by magnificently, the great stallion leaping high in his restrained vitality. The outpost promptly levelled his rifle. We saw the vivid flash in the half light. Brower reeled in his saddle, half fell, caught himself by the stallion's mane and clung, swinging to and fro. The horse, freed of control, tossed his head, laid back his ears, and ran straight as an arrow for the great doors of the ranch.
We uttered a simultaneous groan of dismay. Then with one accord we struck spurs and charged at full speed, grimly and silently. Against the gathering hush of evening rose only the drum-roll of our horses' hoofs and the dust cloud of their going. Except that Buck Johnson, rising in his stirrups, let off three shots in the air; and at the signal from all points around the beleagured ranch men arose from the brush and mounted concealed horses, and rode out into the open with rifles poised.
The stallion thundered on; and the little jockey managed to cling to the saddle, though how he did it none of us could tell. In the bottomland near the ranch he ran out of the deeper dusk into a band of the strange, luminous after-glow that follows erratically sunset in wide spaces. Then we could see that he was not only holding his seat, but was trying to do something, just what we could not make out. The reins were flying free, so there was no question of regaining control.
A shot flashed at him from the ranch; then a second; after which, as though at command, the firing ceased. Probably the condition of affairs had been recognized.
All this we saw from a distance. The immensity of the Arizona country, especially at dusk when the mountains withdraw behind their veils and mystery flows into the bottomlands, has always a panoramic quality that throws small any human-sized activities. The ranch houses and their attendant trees look like toys; the bands of cattle and the men working them are as though viewed through the reverse lenses of a glass; and the very details of mesquite or sacatone flats, of alkali shallow or of oak grove are blended into broad washes of tone. But now the distant, galloping horse with its swaying mannikin charging on the ranch seemed to fill our world. The great forces of portent that hover aloof in the dusk of the desert stooped as with a rush of wings. The peaceful, wide spaces and the veiled hills and the brooding skies were swept clear. Crisis filled our souls: crisis laid her hand on every living moving thing in the world, stopping it in its tracks so that the very infinities for a brief, weird period seemed poised over the running horse and the swaying, fumbling man.
At least that is the way it affected me; and subsequent talk leads me to believe that that it is how it affected every man jack of us. We all had different ways of expressing it. Windy Bill subsequently remarked: "I felt like some old Injun He-God had just told me to crawl in my hole and give them that knew how a chanct."
But I know we all stopped short, frozen in our tracks, and stared, and I don't believe man, or horse, drew a deep breath.
Nearer and nearer the stallion drew to the ranch. Now he was within a few yards. In another moment he would crash head on, at tremendous speed, into the closed massive doors. The rider seemed to have regained somewhat of his strength. He was sitting straight in the saddle, was no longer clinging. But apparently he was making no effort to regain control. His head was bent and he was still fumbling at something. The distance was too great for us to make out what, but that much we could see.
On flew the stallion at undiminished speed. He was running blind; and seemingly nothing could save him from a crash. But at almost the last moment the great doors swung back. Those within had indeed realized the situation and were meeting it. At the same instant Brower rose in his stirrups and brought his arm forward in a wide, free swing. A blinding glare flashed across the world. We felt the thud and heave of a tremendous explosion. Dust obliterated everything.
"Charge, you coyotes! Charge!" shrieked Buck Johnson.
And at full speed, shrieking like fiends, we swept across flats.


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Everything worth publishing on Novosibirsk. As usually – very skewed to our team's tastes.
Himalayan cubs born in the Novosibirsk Zoo in January, along with their mother, the bear, Chelsea, were transferred to an aviary accessible to visitors. The first two months of life of the cubs did not leave their shelter, and only in March one of the three babies first appeared on the open air. Now the […]read more


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Girl left locked in hot car; mom faces child neglect charge
Girl left locked in hot car; mom faces child neglect charge
Aug. 22, 2018
ORLANDO, Fla. (AP) — Sheriff's officials in Florida have arrested the mother of a 3-year-old girl who was left in a locked inside a hot car.
Orange County Sheriff's spokesman Jeff Williamson said in a news release that someone noticed the child inside the car parked outside the juvenile detention center on Tuesday afternoon and talked her into opening the car door.
Williamson says the person then brought the child inside the building. She was sweating, but didn't appear to be in distress. Paramedics took her to the hospital where she was treated and released.
The detention center staff found 38-year-old Chandra Cuyler and deputies arrested her. She's facing child neglect charges.
Jail records don't say whether she has a lawyer.


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Police News for Monday, August 31, 2009
Man faces license charge
MILFORD - A Framingham man was arrested after a traffic stop on East Main Street Saturday night, police said.
Jose Mizhirumbay, 19, of 29 Webster St., was arrested at 10:29 p.m. and charged with driving without a license and speeding, police said.
Police issue kidnapping, rape charges
MILFORD - Police arrested a person on Genoa Avenue yesterday morning on rape charges.
Police would not provide the name of the individual, who was arrested at 7:57 a.m. and charged with kidnapping and forcible rape.
Woman faces assault charge
FRANKLIN - A North Attleboro woman was arrested on Carol Drive Saturday evening after a domestic dispute, police said.
Linda K. Ervin, 61, of 4 Franklin St., was arrested at 6:19 p.m. and charged with domestic assault and battery, police said.


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Case Study for Pain Course
Categories: Online Courses
In the last article we touched on some of the software for larger universities and institutions. Here we'll go further in depth into some of the software available for smaller situations—for instance one in which you would sell an info product or online course for free or in exchange for income.
Case Study:
We found a great example that combines the latest health trends with learning. It's a self-directed course that people can take for healing back pain. This site is an amazing example of providing an extraordinary amount of information in a course. We've talked about this before, but when you offer your customers much more than what they paid, people keep coming. That's exactly what we found on this site about Tension Myositis Syndrome. It's a back pain condition that doesn't heal through the usual methods.
The folks at www.MindBodySchool.com have produced a high level course you can take with guaranteed results. I recommend you check them out if you're looking for examples, or if you have a bad back.
In addition to delivering great content, they employ a series of videos, multiple infographics and interactive assessments and exercises they call Explorations. Basically, as you go along in the course, you do these personal assignments that either examine your personality or help you uncover things in your life that are less than perfect. It's a great tool to help students get the most out of the program.
Learning Platform: Woo
They've used the powerful and popular WooSensei as the learning platform, which is one of the most popular WordPress plugins. WooThemes has taken the market by storm producing some excellent themes for beginners that are also usable by developers. They've also produced some great plugins, including WooCommerce which has been downloaded over one million times.
About a year ago they came out with another plugin called Sensei. It's only a year old and could still use some spiffing up. I'm not sure it's going to happen based on the boards and what they've marked as "working on", but aside from some of the quiz shortcomings, it looks great. Sensei is designed to work with your theme, so whatever designing and fonts you have now will transfer right over into the course. Very slick and very professional. The downsides are that you can't time the lessons and you can't use survey style questions in the quizzes. This seems like it shouldn't be too hard considering WP Pro Quiz already offers those options for free.


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On a hot Thursday afternoon I find myself playing school taxi to the youngest members of the Naidu family. Within seconds of entering the parking lot, the air is filled with the excited shrieks of children. Fingers are pointed, mouths are agape. You simply could not elicit such a response with any of the Teutonic rivals Maserati's Ghibli competes with. Never mind the fact that they are all more accomplished in virtually every respect. For charisma, presence and sheer theatre, the Italian with the trident-shaped jewellery has them beat.
I am approached by a small boy who asks if he can get in. He eagerly clambers into the supple peanut-butter coloured driver's seat – and you just know the memory will be with him when he becomes a potential Maserati buyer in three decades. That puts it all into perspective. The malaise of the daily slog and growing older – tend to jade a person. But moments like these remind you about the crux of these machines that move us. They are supposed to stir up those childlike sensations of genuine awe and butterflies in the tummy.
And in that sense, the Maserati delivers amply. Let us begin the brief ode by acknowledging those looks. What a stunning thing it is, especially in this bold shade of blue seen in the pictures. People admire the Ghibli wistfully, but it also wields a certain element of intimidation. When you see a Maserati owner, you know the person behind the wheel is not your average Jane or Joe. They could have followed the herd. But they opted to go about flaunting their power and affluence more creatively. Certainly, they would find innovative ways to make your life difficult should you irk them.
What about the sound? Take the potent S model and you get a howling six-cylinder symphony, with a pretty brisk 0-100km/h sprint time of five seconds flat. You can also have a diesel and I have seen a number of motoring scribes gushing like sycophants over how good this engine sounds for an oil-burner. Yeah, right. Now while the Ghibli represents champagne motoring, there are some sections more akin to cheap beer. Far too many elements have been lifted from the parts bin of the Chrysler side of the Fiat-Chrysler family. The digital interface, for example, is exactly the one you would find in a Jeep Cherokee. Even the switches were lifted from those models. The key is also just a fancier looking version of the fob used for the American cousins. Look closely and you will notice that they lifted the side mirrors from a 300C. And this is simply not commensurate with the expectations buyers would have of a car costing between R1.3-million and R1.6-million.
That leads me to the other issue. For the same money you can buy the flagship versions of the cars Maserati tilts the Ghibli at. That includes the BMW M5, Mercedes-AMG E63 and the Audi RS6. In fairness, the latter is only available as a wagon. Despite its incredible presence, perfect aesthetics and delicious sound I am just not convinced that a rational person would take it over one of those polished German alternatives. But then again, Maserati people are a little off the wall. Something that could be well represented by the person they are considering as a brand ambassador, the very eccentric fashion designer David Tlale.
–Brenwin Naidu


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The Noble Research Institute LLC in Ardmore, Oklahoma, is seeking a Full Time Temporary Research Associate 1 to assist with all aspects of lab and field work to support the scientific research objectives of a land stewardship and ecosystem services funded project. The successful candidate will perform research on soils, plants and ecosystems related to land stewardship in the Southern Great Plains of the United States. This position will be under the direct supervision of Shawn Norton with research being supervised jointly between Ag Systems and Technology and the Industry Relations and Stewardship group.
This a temporary position with initial funding through 2019. Funding is anticipated to be renewed annually based upon performance and funding availability for a potential of three years.
Research Associate 1 (Field) Job Description
EOE Minorities/Females/Protected Veterans/Disabled
The responsibilities for this position include but are not limited to soil, vegetation and sampling analysis, of land stewardship field experiments. Also, this position will support the work of research with both Ag Systems and Technology and the Industry Relations and Stewardship efforts.
Bachelor degree in range/pasture management, agronomy, soils, agriculture, or related field is required. Excellent written and oral communication skills are also required. Training in soils and some experience working with sampling is preferred. The successful candidate should be self-motivated to work independently and coordinate successfully with team members.
Because of the physical demands of this position, the selected candidate will be required to successfully complete a work steps test.
Software Powered by iCIMS


text: "It is clear to me there is overwhelming evidence," Judge John Hlophe said in the Western Cape High Court on Thursday."[It is] clear from DNA evidence that you are not the mother. One doesn't have to be a rocket scientist to know you don't buy babies."Read more: 'I had to listen to her lies for days': Zephany judgeThe accused, who may not be named as it may result in the teenager's identity being revealed, pleaded not guilty to charges of kidnapping, fraud and contravening the Children's Act.On Wednesday, Judge John Hlophe questioned the women's testimony, saying no one would believe her claims.Read more: Zephany Nurse's birth mother breaks down in courtHe dismissed her evidence, saying he had "listened to her lies for days"."She was consistent. She bought the baby for R3 000 and paid a deposit of R800, but with no receipt. Who can believe that?"According to the accused's testimony, she got the baby from a woman called Sylvia, who had been giving her fertility treatment in 1997 after she had miscarried.Sylvia arranged for the baby to be handed to her at Wynberg station without her prior knowledge, the accused claimed.She said she was surprised because she had expected to meet to discuss the adoption process.Read more: 'I'm guilty of raising her, yes. I raised her with the help of God but I didn't take her': Zephany's alleged abductorSylvia claimed she knew of a woman who did not want to keep her baby because the pregnancy was unplanned.The accused testified the woman said the paperwork would be arranged later and she had signed documents including an "adoption application".She claimed to have never heard from Sylvia again.The accused said that six years later she received a birth certificate by post with the name she chose for the baby.Morne Nurse, Zephany's biological father, previously testified he collected evidence for a month after he met the teenager, when his daughter told him a girl at her school bore a striking resemblance to her.After collecting his daughter Cassidy and the then 17-year-old from school in January 2015, he took the two friends to lunch, where she told him her date of birth – April 30, 1997. This was the date his daughter was abducted.When he told her she did not look like her parents and looked just like him and his daughter, she burst out laughing.He backed off as he did not want to scare her, but "scrutinised" her Facebook account and printed photos of her as she had grown up.Nurse said he sent a photo of the girl and her mother to witness Shireen Piet, who claimed to have seen the suspected abductor on the day Zephany was snatched from her sleeping mother.When he asked Piet if she recognised the woman, Piet said she did.DNA tests later confirmed the teenager was Zephany.News24


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National Geographic : 2008 Sep
MATERNAL DISAPPROVAL Males will attempt to mate with any female in estrus, but a large bull can injure a small female when mounting her-breaking one of her legs, for instance, beneath his weight. After a three-day pursuit, Leopold tries with an eight-year-old from the First Ladies and is thwarted by her mother, Mary Todd Lincoln, and other females. Later, Mary Todd sanctioned her daughter's mating with a younger, smaller bull. SHOWING RESPECT Boone, a formidable bull who spends most of his time east of the Samburu reserve, visits the Poetics and is met with embraces by Maya, the matriarch, and her eldest daughter. This is not, or not directly, about sex; neither female is in estrus. They seem to be merely expressing appreciation for an estimable individual, a fine male, who probably contributed genes to their lineage and may again. LEISURE TIME Two of the Royals get afternoon shade, and a bit of scratching, beneath an acacia near the river. Their family is one of the more dominant, giving them first claim on good habitat. Dominant families tend to spend more time within the reserve and along the river and occupy smaller home ranges. Less movement through insecure areas means less exposure to risks such as falling afoul of farmers and herders. ADOPTIVE MOTHER Saturn, an elderly mother herself, cares for two calves orphaned by the deaths of their mothers, who were her adult daughters. The extended family structure helps groups cope with such losses. At sunset along a river channel Saturn oversees the play of her own two calves and the orphans. From her behavior alone, uniformly doting, you couldn't know which was which.
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Even though there were only 3 days of school last week, we made the most of them! Our week was capped off by a very successful Veterans Day Assembly! We were so honored to have Veterans visit us from around the area to connect with our Student Council. In fact, we had a Veteran visit us who was 93 years old! She was so impressed with our school! We would like to thank Mrs. Kern, Mrs. Gehringer, and Mrs. Hoffman for coordinating the luncheon and assembly!
Can you believe that the first marking period is over on Monday, November 13? On Tuesday, the students will be going to their next elective. Report cards will be distributed next Wednesday before the Thanksgiving holiday. Also, please note that we will be having Make Up Pictures this Friday!
Video of the Week - Check out this inspiration video (only about 7 minutes) about everyday leadership opportunities! Thanks to Ms. Carroll for recommending!
Daily Announcements
|Mr. Dodge's favorite tree
6th: 10 Days
7th: 7 Days
8th: 0 Days
Congratulations to 6th grade for earning your Free Seat Friday! Keep up the good work!
Did you buy or sell items for the Lakeside Cherrydale Fundraiser? All items arrive next Thursday, November 16th. Stay tuned for more details on pick up!
Our grand prize winner was announced but who will win the winners choice drawing? Stay tuned!!
The cast list is up for Seussical! It is posted on the choir bulletin board.
Congrats to all who will be a part of our cast. Crew lists will be available soon.
Are you interested in being involved with the Audio/Visual Club at Lakeside Middle School? Would you like to write articles, take pictures and video for the school at the events? If you answered yes to any of these questions, please come out to the AVA Club Meeting on Friday, November 17 at 3:20PM - 4:20 PM in the DMC. Dr. Cook, who will be the AVA Club Advisor will go over the club expectations and have students get started with ideas! If you have any questions, please see Dr. Cook in the Main Office.
|Ms. Grennon and Mrs. Raugh at the NJEA Convention!
Any Student interested in Running the Jolly Holly 5k and 1 mile fun Run before the Millville Christmas parade, please see your PE teacher for a form. All forms must be handed in to Coach Camerieri.
Thank you and our today joke of the week is... You know, mountains aren't just funny…they're hill areas. Have a wonderful Week at LMS!
Pictures from around the school
|6th grade lab time
|Too cold to walk outside? Join the Walking Club
|Mr. Williams's students solving a crime
|Mr. Williams's students solving a crime
|Mr. Williams's students solving a crime
|Veterans Day Luncheon
|Veterans Day Luncheon
|Veterans Day Luncheon
|Students preparing their speeches
|Thank you to all the Veterans who joined us!
|Students assembling for the Veterans Assembly!
|Dr. Cook backstage with the speakers
|This needs no caption
|USA All the Way!
|Our special guests
|Yummy food at the luncheon!
Winners of the Week - This week's winners are Mrs. Kern, Mrs. Gehringer and Mrs. Hoffman for their coordination of the Veterans Day Luncheon and Assembly! Thank you for going above and beyond for our Veterans, Student Leaders and Lakeside!
|Mrs. Gehringer, Mrs. Hoffman and Mrs. Kern
Weekly Overview
Monday - 11/13 - A Day - Marking Period 1 last day; Phys Ed Club, Credit Completion 3:20 PM
Tuesday - 11/14 - B Day - Marking Period 2 starts, Health Screenings, Cheer Club, Library Club, 3:20 PM
Wednesday - 11/15 - A Day - 6th Grade Dance, 3:20 PM
Thursday - 11/16 - B Day - Shades of Blue, Lego Club, Homework Club, 3:20 PM
Friday - 11/17 - A Day - Make Up Picture Day


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Oh you're a quilter? When anyone says anything like that to me, I stiffen up and expect the worse. Here is my view of what happens next!
Sew happy!
I hope you enjoyed reading this and will keep reading my blog. To do so, type your email address in the box and click the "create subscription" button. My list is completely spam free, and you can opt out at any time.
Filed under: Quilters have personal lives too!


text: i'm a writer, artist, and i experiment with music. currenly in senior year. my main goal is to publish my book series that ive been developing for the past three years called cosmosis, so that's what a majority of my social medias are dedicated to. always feel free to ask questions about it or draw/write/do whatever for it if you want to! and dont hesitate to strike up a conversation either, im very friendly i promise, although i must warn that i often oscillate between not talking for long periods of time/bland sounding/long delays in responses and dumping twenty metric tons of screaming and keysmashes


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Sri Mas Ballroom, Level 4, Bayview Hotel Georgetown Penang, 25A Farquhar Street, 10200 Penang
We wish to announce that all the resolutions set out in the Notice of the Fiifty-Fifth Annual General Meeting ("55th AGM") of Oriental Holdings Berhad ("the Company") held on 8 June 2017 were duly passed by way of poll. The results of the poll were duly verified by Boardroom Business Solution Sdn. Bhd., the independent Scrutineer appointed by the Company.
Kindly refer to the attached file for the outcome of the 55th AGM.
Please refer attachment below.


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There are a lot of people that have spine problems and we should know that there are specialized treatments that are given to our spine. There are different kinds of problems that we could have with our spine as we may have injuries on our spine because of accidents and there are also cases where it would be caused by a bad posture that we have when sleeping or when we are sitting. We should know that our spine would contain a lot of nerves that are responsible for the functions that we have in our body as it distributes the signals that would be coming from our brain. Injuries in our spine would surely cause us a lot of problems as it may lead to our paralysis. We should look for the proper specialists that we need for our spine problems so that we can be sure that the treatment that we are going to get would be effective. Chiropractors are medical specialists that deals with spine problems and they can give us the therapy that we need in order for us to recover. We should know that there are different kinds of procedures that are done in a chiropractic therapy as it can be quite sensitive to deal with injuries or problems that we have in our spin.
In getting a chiropractic therapy, we would be first have an x-ray so that the problem would be properly assessed. There are different kinds of treatment that would be given as there are therapeutic massages and physical activities that would be able to bring the sensation back into our muscles. The physical therapy that we are going to get would be done for a certain period of time as it may take a while for us to recover from spinal problems. If the injuries or the problems that we have in our spine would be considered serious, we should know that we would need to get a surgery so that we can have the problems in our spine fixed directly. We should know that there are facilities that we can go to that can give us a complete treatment for our spine problems and it would be best if we are able to deal with them. There are a lot of ads on the internet and listings that we can find on chiropractic care facilities such as
Active Health
and that is why it is important that we should be able to do some research.
See more
on this link:
Taking Care of Your Spine


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