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Bra, Italy 2011: A Visit to "Cheese"
Every two years, the biggest festival in the cheese world happens in Bra, Italy. The event is known simply as "Cheese." Cheesemakers, cheesemongers, journalists, food lovers and folks lucky enough to live close by, descend on the small town of Bra to sample, sell and eat literally tons of cheese. This year at the biennial festival it was no different. With one exception. The thermometer hit a whopping 90°F.
"Cheese" is my favorite food event to attend. Everyone is there and, while it is a lot of work, it gives us a rare chance to catch up with cheese friends from all over the globe in one place.
Our home away from home during the festival is Cantine Ascheri. Owner Matteo Ascheri is a big bear of a guy, an excellent winemaker, and our personal guide for all things Piemontese. In the years we have known him, he has introduced us to many of the food artisans we still work with today.
After getting settled at the hotel, our next stop was lunch at Matteo's Osteria. A meal of gnocchi with Castelmagno, a veal chop, delicate raviolis and a bottle of his Pelaverga wine jump-started our day after the long flight.
After lunch, we drove to the small town of Bubbio in the province of Asti to meet up with a local robiola producer and sample his cheeses. Kurt and David, lead cheese buyers for our Cambridge and South End locations, joined us there. They had just driven down from the Jura where they tasted through our fall/winter selection of Comté and attended the opening party of the new caves at Marcel Petite. After a few hours of cheese, wine, amaretti and torrone (whew!) we drove back to Bra. The next day would begin at 7am with more meetings and tastings throughout the day so we needed a good night's sleep.
One of the events we look forward to at "Cheese" is the dinner hosted by Giorgio Cravero. Giorgio ages our stellar Parmigiano Reggiano and, to kick off the Cheese festival, he invites a small group of friends to dinner at a local restaurant. The meal feels like a family reunion as many of our closest international cheese friends are there, including Jason Hinds and Randolph Hodgson of Neal's Yard Dairy, Philippe Goux from Marcel Petite, Will Studd, a filmmaker from Australia, Betty and Martin Koster from L'Amuse, among many others.
When we were not moving around the restaurant to catch up with friends, we enjoyed a sampling of classic Piemontese cuisine including salsiccia di Bra, carne cruda and agnolotti al plin with butter and sage. Wine flowed throughout the night and, at the end of the meal, the outdoor patio attracted a collection of cigar lovers.
The next day we walked the city of Cheese in the scorching sun. Undeterred by the heat, we tasted through hundreds of cheeses – standing most of the time and moving along until something caught our attention or we were invited to sit (thank goodness!) and have a glass of wine or beer at a friend's booth.
After a successful day at the show and a quick rest at the hotel, we made another regular stop to have dinner with the Marino family in the town of Cossano Belbo. Mulino Marino is a family-run mill that stone-grinds organic grains to give us our tipo 00 flour and our incredibly flavorful polentas. We drove an hour over winding roads to get there and, despite the numerous times we'd been before, we inevitably had to stop at a few unsigned intersections to make sure we were on the right track.
Dinner with the Marino family is always a long evening with an abundance of food and drink and an incredible feeling of Famiglia. Maria-Teresa and Gabriella prepared a feast this year that began on the terrace with Enkir flour cakes and salumi with a local sparkling rosé. We moved inside and began with a giardiniera; a salad of picked vegetables, tomatoes, white beans and tuna as well as their own pickled porcini served with a local Dolcetto; then we moved onto a homemade pasta made with their Enkir flour and served with a ragu sauce and Barbaresco.
After a moment wondering whether we could go on, our appetites reappeared when the polenta (slowly cooked for 3-4 hours) appeared alongside slices of braised beef shank served with a Clerico Barolo 2005 and a Cappellano Pie Franco Barolo 2005. It was a symphony of food, all the while with multiple conversations flowing around the table in Italian and English and some a combination of the two.
As the meal was winding down, we ate freshly sliced peaches with homemade ice cream, and a pear torte. The party moved to the terrace again when the men decided to smoke cigars and try the local beer brewed with their Enkir flour (something we just started carrying at the shop) and then we all had various grappas from the legendary Romano Levi. Around 1:00am, we really had to go. Incredibly, the ride home went by without a single stop to find our way. We were soon back in the comfort of Matteo's hotel.
Another day at Cheese was spent catching up with old producers like Maria from Caseficio Caggiano. She shared some of her amazing spicy salami with us – unfortunately, we can't sell her salami (due to import restrictions) but she is going to send us some of her ground dried pepperoncini, so be on the lookout for it in our shops soon! Over the course of the day, we implemented a divide and conquer strategy to cover a lot of ground.
Now, back home, we can't wait for our new finds to arrive: tajarin pastas will be arriving just in time for winter truffle season, there will be new amaretti, honeys, Enkir flour cakes and rye pasta coming in over the next few months. Keep an eye out in our stores and on Facebook and Twitter where we'll note their arrivals.
On our trip, it does seem a bit like all we did was eat and drink – consider it quality control – all part of the job!
Valerie Gurdal is the owner of Formaggio Kitchen South End.


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Valvula is a high performance mail policy daemon for Postfix. It provides out of the box support for sender login mismatch, mail quotas, per user and per domain blacklists and whitelists, and much more. It is system administrator friendly, providing many automatic features like automatically detecting Postfix MySQL databases to determine local domains and local accounts. It is fully extensible through plugins.
dput-webdav is a "dput" upload method plugin with extended WebDAV support. It was written specifically with Artifactory and Bintray in mind. It tries to be compatible with the "http" plugin, but offers additional features: login credentials can include the password, "incoming" can be a full URL (optionally containing dynamic package metadata, and supporting HTTPS), and distributions can be mapped to repository names.
Limbo is a PC Emulator (x86) based on QEMU for Android devices. It lets you run a Desktop OS like Debian or DSL Linux on your Android device with Network and X Windows. Limbo works with Live CD ISOs and pre-installed hard disk images created with QEMU, VMWare, Bochs, or Virtual PC emulators.
Booktype makes it easier for people and organizations to collate, organize, edit, and publish books. Delivering frictionlessly to print, lulu.com, and almost any ereader, Booktype facilitates collaborative production processes, with no more lost manuscripts, overwritten Word files, awkward wikis, or cumbersome CMSes.
UCS is a reliable, pre-configured Linux server operating system featuring: Active Directory-like domain services compatible with Microsoft Active Directory; a mature and easy-to-use Web-based management system for user, rights, and infrastructure management; a scalable underlying concept suited for single server scenarios as well as to run and manage thousands of clients and servers for thousands of users within one single UCS domain; an app center providing single-click installation and integration of many business applications from 3rd parties and Univention; management capabilities to manage Linux- and UNIX-based clients; and command line, scripting interfaces, and APIs for automatization and extension. Its purpose is to provide Microsoft Server-like services on the cloud or on the premises, to run and operate corporate IT environments with Windows- and Linux-based clients, and to extend those environments with proven enterprise software.
pyBit uses AMQP to create a distributed, cross-platform buildd toolkit to build packages using a collection of buildds, direct from various VCS clients. It is intended to support rapidly evolving software collections, and can support multiple VCS frontends and multiple build backends. Cross building is expected to be supported for some backends. The initial backend uses dpkg for Debian. pyBit includes support for cancelling selected builds and using multiple buildd clients per architecture, per platform, and per suite.

subset: commoncrawl


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Natural selection is proven wrong
Discussion of everything related to the Theory of Evolution.
Moderator: BioTeam
You do realize the Cambrian era was quite a few years ago? Did it ever occur to you that maybe, just maybe, we don't know all about the environment and species living that time? Just because it looks more difficult to explain than certain other periods in history doesn't disprove evolution or natural selection.
There are several reasonable explanations for this event that are in line with the theory of evolution. For example, each year there are findings of pre-Cambrian organisms that have been unknown this far and may prove to be the ancestors of those more advanced organisms that allegedly "appeared from nowhere". The whole phenomenon has possibly been a sum of many factors and only because we do not know all of them (yet) hardly gives a reason to doubt natural selection.
Actually Wikipedia, which you so eagerly keep quoting, lists many factors that help to explain the Cambrian explosion. Maybe you should look at the whole picture instead of choosing few isolated lines or chapters that seem to support your view when taken out of context.
And the problem with this would be???
Generally speaking, the more people talk about "being saved," the further away they actually are from true salvation.
#2 Total Post Count
Re: Natural selection is proven wrong
read dean
Your entire argument is based on natural selection only being able to work on DNA that is already present in a population, but this is where mutation comes into play for creating new genes for natural selection to work with. Thus you entire argument is like a castle built on sand, doomed to collapse.
Generally speaking, the more people talk about "being saved," the further away they actually are from true salvation.
#2 Total Post Count
Re: Natural selection is proven wrong
fact is speciation a new species has new genes not present in the antecendent species so they could not have been passed on as NS says -thus you own words show NS is wrong
gamila, let me try to walk you through this. Evolution is based on two principles: descent with modification and natural selection. "Descent with modification" just means that new muations are inherited from parent to offspring. Darwin didn't know the source of modifications, but modern genetics understands it well. New mutations in DNA happen all the time. Errors during the replication of DNA when a cell divides, radiation from a variety of natural and man-made sources, various chemicals in the environment - these can all cause mutations. When they happen in germ cells (sperm and eggs in sexually reproducing organisms, or anywhere in unicellular organisms that reproduce by simply dividing), these new changes are inherited. Now, natural selection. This seems to be where you are having trouble. You say that natural selection only acts on traits that are already present. You are absolutely right. But you must admit that there is variation within species. No two people are the same. Dogs are a good example, too. Look at all the different breeds within a single species. Where did this variation come from? Mutations. One here, one there, one now, one later. They accumulate in populations over time. Long periods of time. This is what natural selection acts on. Some individual or combinations of variations help an organism to survive and reproduce. Some don't. Most are pretty neutral. Nature selects those that help, and weeds out those that don't - perhaps not quickly, but statistically over time, this is what happens. New species don't arise overnight. Imagine a species that has a large range where environmental conditions vary (including purely physical conditions such as climate, and nonphysical conditions such as the community of other species present). A population of that species in the eastern part of that range lives in an environment that is somewhat different than what a population in the western part of the range lives in. "Nature" is different in the east and the west. Natural selection will therefore select for different traits in the two populations that are already present in the species. These two populations will therefore become somewhat different over time. If the flow of genetic material (sex) between these populations is somehow prevented, the two populations will continue to diverge genetically to the point where if they were to meet again, their genomes are sufficiently different that they can no longer interbreed. By our definition, they are different species. Voila.
Now, the Cambrian explosion. This "explosion" occurred over a period of 40-50 million years. It's called an explosion because relative to other geological eras, a lot of new forms did indeed appear. If you look at the fossils from one site, e.g. the Burgess shales, you do see forms appearing "suddenly" without evidence of intermediate forms in lower strata at the same site. This is probably because conditions at that site were not conducive to the preservation of fossils in the lower strata. You must try to understand how fossils are formed. Not every organism that dies gets fossilized. You need an environment where sediments can cover a dead organism (quickly) and conditions that will prevent its complete destruction once buried. This doesn't happen everywhere at all times. The fossil record is very spotty indeed. Dead organsims only get fossilized here and there, now and then. And we certainly haven't looked everywhere on this planet. The search for intermediate forms is very slowly finding a number of "missing links". People used to claim that the gaps in the fossil record between reptiles and mammals, reptiles and birds, apes and humans, etc., etc. were evidence against evolution. These gaps, though, are slowly being filled by new discoveries. We've only been at this fossil-hunting game for a very, very short time. More and more fossils from the Cambrian are being found from periods earlier than those where many new forms "suddenly" appeared. But nowhere (yet) has anyone found a single site representing the entire Cambrian that might contain all early, intermediate, and later forms.
So please have patience, and an open mind.
Re: Natural selection is proven wrong
i am not talking about variations in a species but complete new species with complete new genes the new species differs from the older species because it has new genes-never before seen
thus NS can account for it as it only deals with genes already present
http://conservativecolloquium.wordpress ... evolution/
Re: Natural selection is proven wrong
before you can talk about speciation you have to know what a species is
and there is no agreement
so you cannot argue as you all do that speciation does not make NS wrong- untill there is a definition
http://www.pubmedcentral.nih.gov/articl ... rtid=22088
now purely from the NS formulation speciation shows it to be wrong
ie NS is about genes already present being passed on
as such
it cant account for a completely new species-with new genes never seen before
http://conservativecolloquium.wordpress ... evolution/
And where do you think these new genes came from? Mutations!
I find it interesting that you dropped your argument about cancer almost as quickly as that was explained to you. In fact you still haven't answered my question as to what (if anything) you found wrong with my analysis. Have you conceded this point or are you simply neglecting to answer it? For that matter, you also haven't presented any legitimate scientific sources to verify your claims. As much as I love wikipedia, it doesn't even come close to counting as a scientific source.
Another question I would like to ask is what theory you wish to propose in place of natural selection, and what evidence you can put forward for it.
Generally speaking, the more people talk about "being saved," the further away they actually are from true salvation.
#2 Total Post Count
Re: Natural selection is proven wrong
what is this
Re: Natural selection is proven wrong
It's an ABC News article, written for a popular audience, that just happens to have absolutely nothing at all to do with natural selection.. By "legitimate scientific source," I was thinking more along the lines of a peer-reviewed journal that is actually relevant to the topic of our discussion. Nature would be a good place to start, but there are plenty of others. Heck, I'd even settle for an article from Scientific American or National Geographic at this point. Even if they're not peer-reviewed journals, they at least usually make an effort to ensure that their articles bear some resemblance to verifiable facts.
Generally speaking, the more people talk about "being saved," the further away they actually are from true salvation.
#2 Total Post Count
Re: Natural selection is proven wrong
if you followed the link you will see it is legitamate
http://www.abc.net.au/science/articles/ ... 529713.htm
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Death Notices for Dec. 13, 2013
Gilbert W. "Gil" Bergdoll, 92, Janesville, died Thursday at home. Services will be at 11 a.m. Monday at Mt. Calvary Lutheran Church, Janesville. Visitation will be at 1 p.m. until 3 p.m. Sunday at Whitcomb-Lynch Funeral Home, Janesville, and again from 10 a.m. until the time of services Monday at the church.
Linda M. Gabbard, 64, Beloit, died Wednesday at Beloit Memorial Hospital. Memorial services will be from 3 to 6 p.m. Saturday, Dec. 21 at the West-Field American Legion Post, 48, Beloit. Hansen-Gravitt Funeral Home, Beloit, is assisting the family.
Margaret M. Schweitzer, 83, Williams Bay, died Thursday at the Golden Years Care Center, Walworth. Services will be at 1 p.m. Saturday at Calvary Community Church, Williams Bay. Visitation will be from noon until the time of services Saturday at the church. Steinke Funeral Home and Cremation Services, Lake Geneva, is assisting the family.


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What's going on here? Workers preparing temporary lanes on Interstate
TOWN OF LA PRAIRIE—Orange barrels and heavy equipment are out in the median of Interstate 90/39 south of Janesville, part of early work to get the area ready for a large Interstate expansion project.
Crews are widening the southbound lanes of the Interstate temporarily, a project that began earlier this summer, said Steve Theisen of the state Department of Transportation.
The larger $826 million Interstate expansion project will force both directions of traffic to travel in the southbound side while construction is underway on northbound lanes.
That means crews need to widen the road to accommodate two lanes each for north- and southbound drivers, Theisen said.
Crews with backhoes and bulldozers will be moving earth and widening the Interstate until the project's scheduled completion date in November.
You might see the signs of construction driving on I-90/39 between Town Line Road and Shopiere Road in the town of La Prairie.
And you'll certainly notice it at night, when lane closures during less-heavy traffic hours help crews get more work done, Theisen said.
"When they're all in their beds and sleeping, the real big stuff (is happening)," he said.
Those closures happen between 7 p.m. and 5 a.m. and haven't caused too many delays for drivers, Theisen said.
"So far, it's been going well with minimal backups or delays," he said.
Crews from Rock Road Co. recently finished the first two-mile section of temporary widening, from the Highway 11 overpass to Town Line Road, Theisen said.
Temporary widening is moving along on schedule, he said.
The larger expansion project is set to officially begin in 2015 and eventually will widen the road to three lanes of traffic in each direction--and four lanes through Janesville--when complete in 2021.
If you want more information about the temporary widening and the larger I-90/39 expansion project, Theisen recommended visiting www.I39-90.wi.gov and signing up for email updates. You can also "Like" the project's Facebook page, "Wisconsin I-39/90 Project."


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Traffic moving smoothly after accident
Friday, May 2, 2014
JANESVILLE—Traffic is moving smoothly after an accident at 6:25 p.m. Friday.
An accident took place on Interstate 39/90,� just north of the Highway 26 exit on Janesville's north side.� The accident, which took place in the median, caused backups in both directions, according to a news release from the Wisconsin State Patrol.�
By 7:17 p.m.,� traffic was moving again, according to the news release.
The accident didn't result in any serious injuries, and no further information will be released, the State Patrol said.


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Delavan-Darien School Board will consider another referendum
DELAVAN—Cuts in the short term and the possibility of another referendum in the long term.
That's what the staff and residents of the Delavan-Darien School District are facing in the upcoming months.
On Monday, the Delavan-Darien School Board will meet in closed session at 6:30 p.m. followed by an open session meeting in the auditorium of the Phoenix Middle School.
At the meeting, the board will announce what the cuts will be.
Also on the agenda: discussion of an Aug. 12 referendum.
On April 1, school district voters rejected a referendum that would have allowed the district to exceed revenue limits by $2.1 million on a reoccurring basis. It failed on a vote of 1,727 to 1,163.
Before the vote, District Administrator Robert Crist warned that a failed referendum would mean significant budget cuts, the equivalent of 30 to 32 teaching positions.
On April 7, the board met in closed session for nearly three hours to consider options.
Crist wants to announce cuts to be made so staff will receive their preliminary non-renewal of contract notices by April 15.
In some cases, the cuts will mean rearranging duties of existing staff, he said.
"It's going to have a domino effect," Crist said.
Hiring in the past several years has been in response to state mandates and to help the district improve its test scores and other measures of achievement.
Crist is worried that the losses will reverse the progress the district has made but at the same time thinks the board has "made a pretty good plan" to balance the budget.
What's next?
"I can tell you this right now, I'm going to recommend going to another referendum," Crist said.
Perhaps the district would ask for a different amount of money or ask for it on a non-recurring basis. It would be up to the school board to decide the details and the timing.
He's not sure what the answer is.
"I just don't know," Crist said. "It's terribly frustrating and disappointing. I feel like we answered the questions."
Last updated: 4:52 pm Friday, April 11, 2014


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As technology advances, the ways in which compliance standards are structured are also changing for many businesses. Regulations require organizations to have ever-tighter management over their business communication, something cloud computing has been able to help with. Solutions have cropped up over the last few years for enterprises looking for ways to simplify their communications control and data security, particularly in the cloud.
eDiscovery is vital for businesses should they become embroiled in legal cases that require swift and accurate presentation of compiled business communication and data. Actiance addresses this need for the simplification of enterprise communication as a provider of compliance, security, archiving and eDiscovery solutions. With the company's product, dubbed Vantage Cloud, businesses can mitigate risk by controlling, monitoring and capturing communications in applications that span brand and provider including Microsoft Office 365, Jive, Salesforce Chatter and public instant messaging channels Google Talk, Yahoo Messenger and AOL Instant Messenger.
Further reading:
How to weave a culture of compliance into the corporate fabric
Bribery prevention: Using controls to strengthen compliance
Can companies hide possible fraud through compliance investigations?
Compliance is, of course, a priority for most organizations, and Vantage Cloud helps businesses keeping their communications entirely in the cloud, from content creation and compliance to archiving and disposal. The advantage here is the lack of need to install hardware or software, and the efficiency that comes with accessing cloud-based portals.
Kris Vann, IG counsel & product marketing manager at Actiance shed some light on how Vantage Cloud helps companies ensure compliance with regulatory requirements in an interview with InsideCounsel:
"Social content communications such as instant messages, forums and chat rooms are now more than ever showing up in regulatory investigations and eDiscovery. Vantage Cloud simplifies the compliance and eDiscovery management of enterprise social content by providing the ability to actively supervise, capture, defensibly dispose, retain, retrieve, and review dynamic social content all in one place."
Reduced risk expenditure is the overall return-on-investment factor that Van notes is specifically targeted by Vantage Cloud. Regulatory requirements such as record-keeping and communications data are met using the service, including information on social business and collaborative platforms. Vann provides an example of what happens when companies fail to meet these requirements:
"Organizations that fail to meet these requirements can be sanctioned. In a high profile case, FINRA fined Barclays $3.75 million for failing to properly supervise and retain approximately 3.3 million instant messages required under regulations. Vantage Cloud guards against this type of risk."
Vantage Cloud is a service that demonstrates the ever-evolving cloud computing market, particularly in regards to the legal and IT departments of businesses. As regulatory requirements advance, so do the technological solutions to meet them. Vann says that the same regulatory requirements to supervise and retain data on-premise now apply to apps and data moving to the cloud. Organizations need to stay on top of these requirements, and meet the increased need to make sure operations efficiency is top-of-mind.
Vann remarked: "The same regulatory requirements to supervise and retain relevant enterprise data on premise, now applies to applications and data moving to the cloud. With the consumerization of IT and proliferation of cloud applications, there is an even greater need for legal IT to get these emerging platforms under control."
Actiance's approach to cloud-based compliance appears to be a forward-thinking solution for enterprises looking to address these compliance-specific needs.


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Idea to determine order of assembly of spliceosome
Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
Moderator: BioTeam
Idea to determine order of assembly of spliceosome
Hi everyone,
I've got this assignment where I'm supposed to present a suggestion of a series of methids to use in order to determine the order of binding of snRNP:s which eventually assemble to a spliceosome.
My initial thought was to use in vitro splicing assay with a couple of extra steps.
2. In vitro transcription with radioactive nucleotides
3. Extraction of pre-mRNA
4. Run agarose-gel where I can identify and roughly determine the size of my marked RNA
5. Add RNA to mammalian cell extracts, e.g. HeLa
6. While the RNA undergoes maturing, I collect samples with regular intervals of time
7. Use phenol to extract proteins from each sample
8. Run MALDI-MS starting with 0 minutes sample. After that, continue with the next sample in chronological order. Somewhere along the line, one of the bars should start to increase in mass (we assume that we don't know the mass or other physical properties of the snRNP:s, thus the "target bar" is unknown to us prior to the appearance of incremental increase of mass). The mass should continue to increase with specific amounts during the maturation. Re-running this test should show a similar pattern of mass increase at specific points in time if it is the snRNP:s mass increase we are detecting.
What do you think of this? Keep in mind I'm studying on the most basic level, so I've probably got something grossly wrong
Any and all suggestions are more than welcome.
The in vivo transcription will incorporate radionucleotides into all molecules of RNA, so you won't be able to discriminate your transcript. But you could purify it on affinity column. However, that would be quite laborious.
Better would be probably to induce your RNA right in the system you want to study and do pull down MS experiment.
Cis or trans? That's what matters.
However, you asked for order of binding, right? In such I case I suppose you already know the biding partners. Thus you can make knock-outs and see which proteins will be bound to pre-mRNA
Cis or trans? That's what matters.
Re: Idea to determine order of assembly of spliceosome
The thing is that the questions are made so that we will pretend that we don't know that much about the spliceosome as we actually do, and from there proceed to map its function and so on. I should have written this more clearly.
In essence, that means I "don't know" the binding partners, only that there is some kind of complex performing the splicing. I want to know how this complex forms, and how many subunits comprise it.
Do you propose I run a pull down and then use MS? When doing the pull down, how do I go on about getting antibodies for a completely unknown protein (we're pretending its unknown)?
I could use knock out, but that would require me to know the corresponding genes, which I don't (pretending, yet again). Also, wouldn't knock down of genes of such high importance cause all kinds of trouble in the cell; probably death before the spliceosome even starts to assemble?
You could do pull down with binding to antisense RNA (or DNA would be better). With this you should get at least some proteins. These you can use subsequently for further identifications.
For such important proteins you can use inducible knock-out.
Cis or trans? That's what matters.
Re: Idea to determine order of assembly of spliceosome
How would I differentiate my target proteins from the rest of the proteins? Do you mean that I should use DNA/RNA that matches the pre-mRNA and that way isolate the target pre-mRNA along with the associated snRNP:s?
That's OK. You're just trying to learn something (and at least you're trying opposite to others
Anyway, yes, using either specific or unspecific NA probes will show you which proteins bind to nucleic acids. The question is, how to discriminate those bound in spliceosome from others. One way could be specific binding to introns (although that could show proteins bound to gDNA), but with that you could have false results because of one-case-only binding/non-binding.
To explain - imagine you will design probes for intron in gene for actin. Generaly there are five proteins in spliceosome, let's call them Spliceosome Protein1 - 5, but in actin is not present SP3, but are present other proteins, let's call them SP6 and SP7. Thus if you try to generalize your result, you will get wrong number AND wrong proteins. To avoid this you probably would have to try several different introns in several genes.
Cis or trans? That's what matters.
Aha ok.
Thank you very much, I just received a mail from my teacher and he actually suggested I do the precipitation, just as you did.
From one thing to another though.
What is the purpose of a bacteriophage promoter being ligated in to a vector along with your gene? Is it just to ensure that you get strong expression if you use some kind of phage RNA-pol?
Forgot one thing:
Say I precipitate the samples at different points in time, how would I go on about analysing them? Is it feasible to use MS here or are the differences (when the next snRNP joins in) in snRNP mass enough for me to be able to detect change in mass on a western?
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Pietro Romanengo fu Stefano: Italian Confectioner
In the list of wonderful, accidental finds we have made for the shop, few rival the discovery of Italian confectioners, Pietro Romanengo fu Stefano. On a trip to northern Italy, Ihsan and Valerie were strolling the labyrinthine streets of old Genova when they stumbled across a fantastical, gem-like little shop.
Since 1780, the Romanengo family has been dispensing handmade candies from this beautiful shop with its marble walls, glass shelves and rich wooden cases. Crystal receptacles are filled with confetti-colored candied fennel seeds, threads of sugar-coated cinnamon, sweet fruit fondants, chewy rosewater marshmallows and tiny pastiglie. On his first visit, Ihsan bought some treats to take home and each parcel was painstaking wrapped in dark blue tissue-like paper and tied with a Genovese sailors knot. Since that trip, we have been importing directly from Romanengo and several staff members have made the pilgrimage to Genova themselves!
A Little History: The Makings of an Italian Confectioner
Pietro Romanengo fu Stefano, was founded by Antonio Maria Romanengo, initially as a colonial goods and drug store. Stefano, one of Antonio Maria's sons, secured a diploma in confectionery from the University of Genova and opened the Via Soziglia store where the company still retains a shop front.
In turn, Stefano's son, Pietro, registered the company as "Pietro Romanengo fu Stefano" and designed the company's distinctive dove and olive branch logo. The logo was intended as a symbol of peace in the wake of the Napoleonic wars. Pietro also introduced the beautiful blue paper packaging used to wrap Ihsan's parcels.
Gradually, the reputation of the Romanengo family's confections began to spread – beyond Genova and beyond the borders of Italy. Among the company's earlier customers was Giuseppe Verdi. His letters discussing Romanengo's candied fruits are kept in the La Scala museum.
Another high point for the company was the 1868 marriage of Prince Umberto to Margherita of Savoy when an order was placed for, "candied fruit, demi-sucres, elegant bonbons and small metal bonbonières decorated with pastilles."
Romanengo today is similar in size to what it was in the late 19th century. The company is run by cousins Paolo, Pietro, Giovanni Battista and Delfina Romanengo. They employ only 24 full-time staff members. Their commitment to small-production is consistent with the strong emphasis that the Romanengo family places on craftsmanship and preserving longstanding confectionery traditions.
Whole Candied Fruits: Gems of the Holiday Table
Among the items we import from Pietro Romanengo fu Stefano are chocolate bars, fruit fondants and rose preserves. At this time of year, we supplement those items with a small selection of their special candied fruits. Each year, Tim consults with Delfina to prepare our order and, invariably, he puts the candied fruit selection entirely in her hands. He says, "Delfina knows our tastes and she knows which fruits are best at that moment." Typical selections include candied apricot halves, peach halves, whole plums, figs and loquats as well as whole clementines – perfect orbs of glistening orange that ooze sugary sweetness when cut in half.
Each type of fruit is given its own sugar syrup bath so the flavors of the fruits are not mixed and each flavor remains distinct. The fruits are gently cooked at a low temperature in the sugar syrup so that the sugar is gradually absorbed into the fruit to complete the candying process. This delicate process can take up to two weeks. Just prior to sale, the candied fruits are glacéed – carefully submerged in another sugar syrup, then quickly drained and allowed to dry. The final product is a perfectly preserved fruit with all of its ripe flavors in a perfect, delicate sugar crust.
The fruits can be enjoyed on their own, but we also like them paired with a variety of our cheeses. Past favorites have included fine pieces of candied clementine blended with lightly warmed ricotta or thin slices of candied fig paired with a fresh chèvre or a bit of the candied plum paired with a ripe Stilton. Each fruit offers its own wonderful possibilities!


text:
The Fiber Disease
Human Anatomy, Physiology, and Medicine. Anything human!
Moderator: BioTeam
Another link to scroll on through. too much info to go through myself, but sky and london, Nadas, Tam, well, have a look. its an excel file and will take a few moments to download.
http://wwwlib.ntut.edu.tw/data/eng/pqdd ... y01-04.xls
"First they ignore you...
Then they laugh at you...
Then they fight you...
Then you win." - Mahatma Gandhi
In the SQUID the current circulates around a ring-shaped circuit made from microscopic aluminum wire. There are two breaks in the loop, each bridged by wires just 60 nanometres wide and made from the semiconductor indium arsenide.(TOLD YA!)
At very low temperatures the aluminum becomes superconducting and the current is carried by pairs of electrons with zero electrical resistance.
The team (PARDON ME??? IS THIS A GD GAME NOW, TEAM????) used electric fields to turn the semiconductor nanowires into "quantum dots" -- isolated islands of electrical charge. Electron pairs can jump to and from the islands, so the supercurrent becomes chopped into discrete parcels of two electrons
Development of DNA-mediated transfection in Entamoeba histolytica will facilitate basic research toward the control of this protozoan parasite. A transient transfection system was established by using the firefly luciferase gene ligated to the 5' and 3' flanking regions of the amebic hgl1 gene. The optimal construct tested encoded an hgl1-luciferase fusion protein and contained 1 kb of 5' flanking sequence with 16 bases of coding sequence from the hgl1 gene ligated in-frame to the luciferase start codon and 2.3 kb of 3' flanking sequence from hgl1 ligated 3' to the luciferase stop codon. Optimal electroporation conditions in strain HM-1:IMSS trophozoites when using this construct were 500 $\mu F$ and 500 V/cm, which resulted in luciferase activity up to 5000-fold above back-ground 9-12 hr after electroporation. Constructs that contained the luciferase gene without amebic flanking sequences or that contained a simian virus 40 promoter, enhancer, and polyadenylylation signal produced only background levels of luciferase activity. The ability to introduce and express genes in amebae will now permit a genetic analysis of the virulence of this organism, which remains a serious threat to world health.
Since sialylation and de-sialylation are vital events in cell survival, the identification of the time signals involved in the regulation of sialylation could be useful in a strategy for drug design.
The present work has shown that sialidase represents an important factor in the pathogenesis of amebiasis and could be an essential target in drug design against the disease amebiasis. We are currently working on the production of monoclonal antibodies against E. histolytica sialidase.
Barnett JEG, Coring DL, Rasool G (1971) Studies on N-acetyl neuraminic acid aldolase. Biochem J 125:275-284
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
Chayen A, Avron B, Nuchamowitz Y, Mirelman D (1988) Appearance of sialoglycoproteins in encysting cells of E. histolytica. Infect Immun 56:673-681
Diamond LS (1968) Techniques of axenic cultivation of E. histolytica schaudin 1903 and E. histolytica-like amoeba. J Parasitol 54:1047-1056
Engstler M, Reuter G, Schauer R (1992) Purification and characterization of a novel sialidase found in procyclic forms of T. brucei. Mol Biochem Parasitol 54:21-30
Engstler M, Reuter G, Schauer R (1993) The developmentally regulated trans-sialidase from T. brucei sialylates PARP. Mol Biochem Parasitol 61:1-14
Feingold C, Mirelman D, Lotan D, Lotan R (1984) Enhancement by retinoic acid of sensitivity of different cell lines to the sialic acid-specific toxin of E. histolytica. Cancer Lett 24:263-271
Feingold C, Bracha R, Wexler A, Mirelman D (1985) Isolation purification and partial characterization of an enterotoxin from extracts of E. histolytica. Infect Immun 48:211-218
Ifyeanyi AU, Leicht JG (1987) A membrane associated neuraminidase in Entamoaeba histolytica trophozoites. Infect Immun 55:181-186
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
Leicht GJ, Dickey AD, Udezulu IA, Bailey GB(1985) E. histolytica trophozoites in the lumen and mucus blanket of rat colon studied in vivo. Infect Immun 47:68-73
Muchmore EA, Varki N, Fukuda M, Varki A (1987) Developmental regulation of sialic acid modifications in rat and human colon. FASEB J 1:229-235
Nok AJ, Uemura H (1998) The Trypanosoma brucei rhodesiense trans-sialidase. Parasitol Int 88:56
Pereira MEA (1983) A developmentally regulated neuraminidase activity in T. cruzi. Science 219:1444-1446
Perkin MA, Rocco LJ (1988) Sialic acid dependent binding of P. falciparum merozoite surface antigen pf 200 to human erythrocytes. J Immunol 141:3190-3196
Ravdin JI (1995) Amebiasis. Clin Infect Dis 20:1453-1466
Roggentin P, Rothe B, Lottspeich F, Schauer R (1988) Cloning and sequencing of a Clostridium perfringes sialidase gene. FEBS Lett 238:31-34
Roggentin T, Kleinadam RG, Schauer R, Roggentin P (1994) Effect of site specific mutation on the enzymatic properties of a sialidase from C. perfringes. Glycoconj J 9:234-240
Schenkman S, Jiang MS, Hart GW, Nussensweig V (1991) A novel cell surface trans-sialidase of T. cruzi generates a stage-specific epitope required for invasion of mammalian cells. Cell 65:1117-1125
Silvia ZM, Julio CV, Carlos CM, Arturo FC, Everardo LR (1999) Entamoaeba histolytica: identification and properties of membrane-bound and soluble α-glucosidases. Exp Parasitol 93:109-115
Stanley SL, Tian K, Koester JP, Li E (1995) The serine-rich Entamoeba histolytica protein is a phosphorylated membrane protein containing O-linked terminal N-acetylglucosamine residues. J Biol Chem 270:4121-4126 http://www.geocities/bacciger
Zoology General WEB http://ar.geocities.com/Zoologia2000
Parasites of jellyfish: http://members.fortunecity.es/Zoologia On construction
http://parasitology.informatik.uni-wuer ... .html.html
Substrate specificity using sialoglycoconjugates revealed strict preference for terminally α-linked sialic acids. Table 3 summarizes the results on substrate specificity analysis. Substrates possessing an α,2,3-linked sialic acid, like Neu5Acα2,3lac, fetuine and bovine mucin, were readily hydrolyzed. The gangliosides GD1 and GM1, having internal sialic acid residues, were unaffected by the enzyme. Also, colomic acid (a homopolymer of α-2,8-linked sialic acid) and Neu5Acα2,6lac were not suitable substrates. Detailed kinetic analysis from initial velocity data revealed Km and Vmax values of 0.059 mM and 769 µM/min, respectively, for Mu-Neu5Ac. The Km and Vmax values for Neu5Ac2, 3lac were 0.144 mM and 2275 µM/min.
Table 3. Substrate specificity of the hydrolysis of glycoconjugate by E. histolytica sialidase. The substrates were adjusted as described in Materials and methods, to contain 1 mM of bound sialic acid in 50 mM acetate buffer, pH 5.5. In the assay using GM1 and GD1, the buffer contained 0.1% Trixton-100. The results are averages of three readings
Substrate Product (Neu5Ac; µU/mg)
MU-α-Neu5Ac 685±25
Neu5Ac2,3-α-lactose 582±30
Neu5Ac2,6-α-lactose 68±15
Colomic acid 30±18
Fetuin 445±35
Mucin 377±35
Ganglioside GM1 30±12
Ganglioside GD1 25±10
The enzyme was also strongly inhibited by Neu5Ac, 2en and weakly by pNPO. Evaluation of the inhibition against Mu-Neu5Ac as substrate revealed competitive patterns (Fig. 3) for both Neu5Ac2en and pNP-oxamic acid with Ki values of 30 µM and 185 µM, respectively. The physiological index of efficiency (Vmax/Km) of the enzyme decreased from 12.94/min to 2.93/min and 4.28/min for Neu5Ac2en and pNPO, respectively, when Mu-Neu5Ac was used as substrate.
Fig. 3. Hanes Wolf plots of initial velocity data of the inhibition of E. histolytica SD-catalyzed hydrolysis of methylumbelliferyl-Neu5Ac (4-MU-Neu5Ac) in the presence of 0.05 mM Neu5Ac2en and 0.2 mM para-nitro-phenyloxamic acid (pNPO). Results are averages of three experiments
Figure 4shows the effect of fetuin on the motility of E. histolytica. At 0.05-0.5 mM fetuin, there was a proportionate increase in the motility of the parasite. In the presence of the sialidase inhibitor Neu5Ac2en, the motility sharply regressed to the non-induced state. Similarly, at 0.05-0.5 mM Neu5Ac2,3lac, there was a sharp increase in the motility of the parasite (Fig. 5). The sialidase inhibitor Neu5Ac2en also inhibited motility when included in the motility-assay cocktail.
Fig. 4. Motility of E. histolytica trophozoites in the presence of different levels of fetuin (FT), the SD inhibitor Neu5Ac2en and a combination of FT and Neu5Ac2en. Results are averages of three experiments
Fig. 5. Motility of E. histolytica trophozoites in the presence of different levels of Neu5Ac2,3lac (SL) the SD inhibitor Neu5Ac2en and a combination of SL and Neu5Ac2en. Results are averages of three experiments
In order to assess the precise role played by the parasite's sialidase, the fluoregenic substrate 4-MU-Neu5Ac was incubated with the trophozoites. A pattern of motility similar to those of Neu5Ac2,3lac and fetuin was observed. This was accompanied by a progressive increase in the level of MU (Fig. 6). A similar increase in the released neuraminic acid (Neu5Ac) was also observed until 15 min. Subsequently, the level started to decrease and this coincided with the emergence of the activity of the enzyme PSL.
Fig. 6. The activity profiles of SD, pyruvate sialate lyase (PSL) and neuraminic acid (NA) of E. histolytica trophozoites incubated in the presence of 4-Mu-Neu5Ac. Results are averages of three experiments. umole Micromoles
In this report, a detailed study was conducted on sialidase from Entamoeba histolytica trophozoites. The purified enzyme is a 65-kDa monomer. like Trypanosoma brucei brucei sialidase (Engstler et al. 1992). Bacterial sialidases have molecular weights of 60-150 kDa (Roggentin et al. 1988). The enzyme has a temperature optimum of 37 °C and a pH optimum of 5.5. It was unstable and lost about 50% activity at 4 °C and was reactivated by re-incubation with dithiothreitol, a reagent that protects SH groups of the enzyme from oxidation. This observation suggests the existence of oxidizable SH residues that cause its reversible inactivation.
The kinetic properties of the enzyme resemble that of other protozoa, like T. brucei and T. cruzi (Schenkman et al. 1991; Engstler et al. 1992; Nok and Uemura 1998), in sensitivity to only α-2,3 linked sialic acids. In contrast however, the enzyme was strongly inhibited by Neu5Ac2en and weakly by pNPO. The increased motility of the parasite in the presence of Neu5Ac2,3-lac and fetuin suggests the involvement of sialidase in the parasite's motility and pathogenesis. The suppression of motility when the sialidase inhibitor Neu5Ac2en was part of the cocktail is further evidence on the role of sialidase in the pathogenicity of the parasite.
Sialoglycoproteins (SGs) have been shown to be important in the enhancement of sensitivity to sialic acid-specific toxin from E. histolytica (Feingold et al. 1984). This toxin is known to induce fluid secretion in ligated intestinal loops of indomethacin-pretreated rats. Since SGs serve as receptors for the E. histolytica toxin, the pathogenesis of amebiasis could be preceded by an initial unmasking event of surface sialic acid by the parasite's sialidase, followed by attachment of the toxin. Feingold et al. (1985) reported the isolation of two cytotoxic factors from E. histolytica trophozoites, one of which is sensitive to sialic acid levels above 5 mg/ml. Our observation could imply that, when such a threshold level of free liberated by sialidase is attained, it triggers the expression of PSL to hydrolyse the sialic acid to pyruvate and N-acetyl mannosamine. This biochemical event may promote the interaction of cytopathic factors with the receptor. Moreover, when Neu5Ac2,3-lac was incubated with intact cells, the level of released freeNeu5Ac decreased with incubation time, coinciding with the appearance of PSL, showing that the Neu5Ac is catabolized. These events could represent signals involved at regulating the level of sialic acid, to avoid the inhibition of the interaction of the cytopathic factors with the receptor.
Both sialyltransferase and trans-sialidase were absent from E. histolytica trophozoites. Moreover, the trophozoites were found not to contain any detectable levels of sialic acid. The lack of sialic acid is consistent with the absence of sialyltransferase and trans-sialidase. Previously, Chayen et al. (1988) reported the presence of sialic acid in cyst forms of E. histolytica. It appears that the uptake of sialic acid in E. histolytica is developmentally regulated, as it also is in the African trypanosome (Engstler et al. 1993). This unique feature could represent an evolutionary linkage between the amitochondrial protist and African trypanosomes. Since sialylation and de-sialylation are vital events in cell survival, the identification of the time signals involved in the regulation of sialylation could be useful in a strategy for drug design.
The present work has shown that sialidase represents an important factor in the pathogenesis of amebiasis and could be an essential target in drug design against the disease amebiasis. We are currently working on the production of monoclonal antibodies against E. histolytica sialidas.
http://www.parasitologyindia.org/journa ... cember.pdf
http://www.genewatch.org/uploads/f03c6d ... report.pdf.
Please explain in laymans terms EXACTLY what this article is talking about and how it directly links to our disease.
If you can not I will believe that you just post and post without any knowledge of what you are posting.
So go ahead, break it down for me in the simplist of terms for everyone here watching this site and then explain how it relates to this disease and what research you are going to do to prove your theory.
This site has become a place where people post and have NO IDEA what they are posting and then the others are conspiracy theorists and nothing more.
Manic postings that is all they are.
In facy I challenge everyone here with these long posts to pick just ONE of them and tell me how you are backing up your reason for posting and how it relates and what you are going tod tto prove how it relates.
The people I have watching this site can't wait to see this one! Simple logical and precise.
Any of the 454 posts will do since none of them compare to the other and they are all over the board with no common thread or plan to prove.
Many people are watching and asking me to ask this question. Pick one post and break it down and show how ti relates to this disease.
Who will be first?
During the End Times, Good will battle Evil. Where do you stand?
Oh, pick me, pick me!!
Well, dah!! I'm kinda thinkin' here that our wonderful world, once upon a time, maybe is no longer. In fact, it is anything but. What does this have to do with it? Everybody's research is proving time and time again, just who we can and cannot trust. It is backing up anybody's doubt as to who has done this or is at least, responsible. Cause who'd ever think it to be true?? We certainly don't want to believe it but hey!! Here you have it. Post after post.
Alright, I pick Nadas last post...How the hell are we supposed to rid ourselves of this whatever fiber...microorganism thing if it's impossible to get our immune systems where they belong. Yeah, I suppose food plays a big part in it, ya think??? Shoot, we are probably feeding the little bastards what they want us to. But we or anyone else for that matter would never know it. Now why do you suppose that is??!!! Cuz it's just one more thing kept a big fat friggin secret from WE THE PEOPLE! This sorry ass medical association is a joke. They make us ill any way they can so they make their $$. The circle of $Life$
Now what do I win, dammit!?
Deena, you got kahonas girl....how bout' a big fat...cigar....naw, how bout' a big fat doob..???? Due to work restrictions I am forced to smoke Valerian...
I have been subscribing to this site for quite a while now...good database...and images..!!! Pick one, any one...
Someone is going to leak classified documents Randy, and I know this will happen, soon. I have prayed ernestly and the Lord just about always gives me what I ask, and in this case, just to PROVE my point, because honestly, I believe you already know.
Conspiracy REALIST.
Mm Link: http://www.foresight.org/Nanomedicine/G ... index.html
Maggie Mae~
And here I forgot the obvious...If chemtrails are getting us, can you imagine what it's doing to all the crops? Shoot, then they get watered, and layered and dusted and watered and layered....and shipped....to stores.....to WE THE PEOPLE. No wonder we can't get a leg up on this.
Randy~Stupid question and now I don't feel like the low man on the totum pole anymore. Thank you.
I really don't want to be a farmer and I certainly don't want my own cow but it does appear to be a little more promising. Just a little, tho.
Don't have to worry about public water.....so much to think about!
sweet lil MM- I liked your link....I spotted those bacteriapahges right off the bat. Looks like there we have our synthetic spider. I think we do have the spider silk in us too.
Hey, well, I think this will kind of back up your link there:
Digital Organisms (duh, the kind they make on a computer)
Programs designed to "evolve" solutions to mathematical problems support the idea that complexity in nature emerges in small, often apparently unremarkable, steps.
Complex biological organisms have long been thought to develop through a series of intermediary evolutionary adaptations, rather than in single giant evolutionary leaps.
Seeing every step along the way in an evolutionary sequence that unfolds over millions of years is of course impossible. But researchers at Michigan State University and the California Institute of Technology in the US have found a way to see this process unfold in its entirety without any 'missing links'.
"Our work allowed us to see how the most complex functions are built up from simpler and simpler functions," says Richard Lenski, a biologist at Michigan State University.
Charles Ofria, a computer scientist at Michigan State University, who was involved with the research, says it may help computer programmers make more efficient evolutionary algorithms.
"One of the beautiful aspects of this work is that it allows us to better understand how nature overcomes difficulties inherent in solving complex problems," he says. "We can then apply these concepts when trying to decide how best to solve computational problems we are faced with."
Simple origins
The researchers created populations of identical "digital organisms", using a computer modeling application called Avida. At the start, each digital organism was incapable of solving logical problems. But with each replication, there was a 20 per cent chance of a random mutation in "offspring". This mutation altered the nature of the digital organism and in some cases resulted in one that could perform a logical operation.
During 15,000 generations or so, the researchers found it was impossible for a population of digital organisms to solve the most difficult logic problems, if that was all that the computer rewarded.
Electronic evolution
But the outcome changed dramatically if the digital organisms lived in environments that would also reward them if they performed some simpler functions. In that case, the evolving programs were able to bridge the gap and eventually solve even the most complex logic problems.
Evidence of a gradual biological evolutionary process is found in complex structures that retain features related to earlier evolutionary steps. The human eye, for example, contains crystalline proteins that are related to those that perform enzymatic functions unrelated to vision.
The researchers say their computer model will let biologists study individual evolutionary steps for the first time. "Darwinian evolution affects DNA and computer code in much the same way," says Christoph Adami, who leads the Digital Life Laboratory at the California Institute of Technology. "This allows us to study evolution in this electronic medium."
Lenski adds that some mutations, which initially looked as if they would not be advantageous to an organism, turned out to be crucial stepping stones in the long run.
Now what I wanna know is why......
what is the purpose of microwaving us with radiation (huh Keely girl?)
when they know good and well wtf it does to us?
It's not nice to fool Mother nature.....and you know what my 2001, five year old genoming book says? Even back then they were working on extending life but Hey, I' m not talking for 20+ years here.....I'm talking about forever.....hell, I do not think I even would want to do that.
Do you guys? The book also discusses the dust>the radipactive dust!
and also how they can reverse enginere the brain and the audiopath which I believe is what they are doing to us.
I like your positive talk MM, I did!
from msc
everyone start reseachings gelatin microbeads, MACS MICROBEADS,
look up webstites where prof kligman used macs microbeads. they used
magnetics used in this and shows exactly what my friend sees the balls with the white worms shooting out of them. look up japanese companies
and their work with kligman, turnkey engineering corp and other corps
there was a website that shows how they are made very lengthly. the pdr book shows edgma causes abberations of chromosones edgma are microbeads and used in polymerization and used in renova and we can't imagine how many other products that we all use, try shampoos, conditioners look at everything you use body washes, medications look up patents they have more chemicals in them apparently then what the labels state on products. i have to go have work early. God Bless you.
Skytroll, this is a very short film for you on biofilms and flagella....it last about 45 seconds max.
http://www.learner.org/channel/courses/ ... ob2_h.html
MSC, thanks, I will. Hey, tonight I read about the deformed frogs from the retoinic acid.....
from msc
one thing more and very very important, we all have to find a product we all used with these types of chemicals in them. print out patent 4877805
on page 7 and 8 list the chemicals used besides lecithin, kobo pigments
and chemicals that are the on the retinoid makeup composition. you need to find out if any medication you used had these retinoids in them
or soy lecithins that cause biofilms anything that had lipids, hydrophilics and lipophilicis, hydrophobics anything that had biological on it. the words EDGMA and HDPE, silicone silica fiber optics. we need to find a common
product or person you came in contact with that used them. everything is about polymerization that is were fibers, threads, ligands plastics all come from being polymerized and look to see if your products use the words nano particles. your patents are the best place to find the words nano and lipids etc. we know it is nano, with know it is plastic silica silicone, was it in medicine we all used, are optic fiber lights a danger to our health things like that we need to find in common. we have to start listing those things we have used and tie it together with the nano research we used. thanks let do this before the holidays get here. lets make it the holiday nobody will forget including those who did this and may they be behind bars before the holdiays get here. that is my goal
they took enough Christmas's from my children they will not take anymore.
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South Shore Cultural Center
7059 S. South Shore Dr. Chicago, Illinois 60649
(773) 256-0149
Fee: Free
Age Range: All Ages
Providing an opportunity for park advisory councils (PACs) to network with PACs across the city in hopes of leveraging best practices and building relationships. The Chicago Park District, Friends of the Parks, and community representatives will be conducting interactive discussions on fundraising, navigating the park district, engaging youth and more! Followed by a panel to share ideas, successes and plans for park safety this summer.


text:
George Wilson, Janesville (1924 - 2014)
April 23, 1924 - January 15, 2014
George H. Wilson, age 89, died on Wednesday, January 15, 2014 at Mercy Hospital and Trauma Center. He was born on April 23, 1924 in Lawrence County, IL, the son of the late Edgar and Nellie (Wright) Wilson. George graduated from Pinkstaff, IL High School and proudly served his country in the US Navy from 1943 until he was honorably discharged in 1946. On November 26, 1946, he married Elois A. Fjelstad and she predeceased him on the same date, nine years ago, January 15, 2005. George was a very active member at First Presbyterian Church, where he ushered for many years.
In his earlier years, George worked as a carpenter for area companies and bartended part time at the former Cordovan Inn, before he took employment with the Janesville School District. George worked at the former Marshall School, maintaining the boiler system for 24 years before retiring. George was a very personable and likable man, who made everyone he met feel like an old friend. He was a very active person, who enjoyed traveling with his wife, bowling, fishing and always proud of his cars. George was also an avid Chicago Bears fan and Cub fan. After retiring, George worked as a funeral assistant for the Whitcomb-Lynch-Overton Funeral Home. He was a member ot the Loyal Order of Moose Lodge #197 and a life member of the VFW Kienow-Hilt Post #1621.
He is survived by his daughter and son-in-law: Judy and Lester Kersten of Brooklyn, WI; two grandsons, whom he was very proud of: Christopher (Diana) Kersten of Monona, WI, and Michael Kersten of Waunakee, WI; three great-grandchildren he adored: Hallie, Ethan, and Gracelynn; several nieces, nephews, other relatives and friends. In addition to his wife and parents, he was predeceased by his six siblings.
Funeral services will be held at noon on Wednesday, January 22, 2014 at FIRST PRESBYTERIAN CHURCH, 17 N. Jackson St., Janesville with Rev. Dr. Bruce Jones officiating. Burial will be at Milton Lawns Memorial Park with full Military Rites provided by VFW Kienow-Hilt Post #1621. Visitation will be on Wednesday from 11:00 AM until the time of the service at the church.
Whitcomb - Lynch Funeral Home & Cremation Services
21 S. Austin Road, Janesville, WI (608) 752-2444


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Glass Candle Cylinder
Create your one-of-a-kind style when you use this elegant and simple glass candle holder with rustic insert. Clear glass creates an enchanting sparkle of candlelight for beautiful luminescence for any occasion.
Dimensions: Large: 6"x 12"; Medium: 6" x 10"; Small: 6" x 8"
Returns Policy
We hope you love what you ordered. In the event that you don't— maybe the fit is not entirely right or the color not what you expected— we're happy to offer you a replacement color, size, or issue a refund on the product within 15 days of the date your order was shipped. Simply e-mail our return department at firstname.lastname@example.com. For your convenience, we can send you a prepaid label to ship the item back to us. A flat $12 will be deducted from your credit for the return package. Postage and handling charges are not refundable on return.
Do you ship internationally?
Unfortunately, we do not ship internationally at this time. We apologize for the inconvenience and appreciate your support.
What happens if I backorder an item?
Backordered items are currently out of stock but will be coming back soon. If you purchase an item on backorder, we will not charge you until the item is shipped. We try our best to estimate when the item will be back in stock, but please understand they are estimations.


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Global companies face global problems. With supply chains that stretch across continents, it can often be a challenge to enforce compliance standards on suppliers and partners in far-flung areas. One company that has learned this lesson the hard way is Samsung Electronics.
The South Korean electronics giant has been met with allegations that one of its Chinese suppliers uses child labor. A nonprofit group known as the China Labor Watch called out Samsung, alleging that the Shinyang Electronics Company, makers or cellphone covers and other parts, was using children who had used fake IDs to get jobs in the factory.
List released of 100 top supply chain projects
Foxconn's compensation debate highlights Taiwan's wage problems
Court keeps conflict minerals filing deadline in place
Samsung had stood firm, denying these allegations in the past, but on July 14, the company announced that it would suspend business relations with Shinyang pending an investigation into this matter.
The impetus for suspending relations with Shinyang seems to be Samsung's own internal investigation into the matter, which was no doubt spurred by the watchdog allegations. Samsung reportedly found evidence of suspicious hiring practices and decided to look into the matter further.
This illustrates the type of problems that companies with large international supply chains face. With literally hundreds of suppliers and partners in countries like China, where enforcement of regulatory standards can be lax, it falls upon the corporations themselves to set the right tone and to ensure compliance. In June, Samsung did an internal audit of some of its Chinese suppliers and found that 59 percent of those polled did not use the proper safety equipment.


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Maryland High School
Career and Technology Education Prorgams of Study
(click here)
Policies & Procedures for theDevelopment & Continuous Improvementof Career and TechnologyEducation Programs of Study
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CTE Task Force Report
CTE Task Force Report - May 2009
Fact Sheets
Career and Technology Education (CTE) fact sheets provide detailed information regarding Maryland programs, products and services developed to support the seamless progression of students from school-to-careers and further education.
1. CTE Fact Sheet
2. CTE Update
3. What Parents Should Know
Inventory Guidelines
The primary purpose of the MSDE-DCTAL Equipment Inventory is to allow all recipients career and technology education funds to accurately and efficiently report equipment purchases.
1. Inventory Manual
Skills for Success (SFS)Maryland developed the Skills for Success (SFS) in order to provide a readily understandable set of skills essential for students' future success in three learning environments: postsecondary education, the workplace, and the community. They were dubbed skills to distinguish them from the knowledge-centered content standards and core learning goals in English, mathematics, science, and social studies. They are descriptions of what students should know and be able to do upon graduation from high school. Skills for Success (SFS)


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Thumbs up/down for Monday, Nov. 18, 2013
Thumbs up to the new Boys & Girls Club of Walworth County. Credit the Delavan-Darien School District for letting the club use Phoenix Middle School in Delavan to host the club from 3 to 6 p.m. Mondays, Tuesdays and Thursdays when school is in session. Credit, too, club organizers for trying diligently since 2010 to raise money for a permanent location, but when that failed being flexible enough to partner with the district. More than half of the district's students live below the poverty line. Providing this positive, structured environment should boost academics, social skills and children's lives in general. The club will be open to ages 7-18, and activities and services will include homework help. "By teaching study habits, offering intensive tutoring and exposure to role models, this Boys & Girls Club will provide these children with a strong foundation on which they can build for success in the future," Gov. Scott Walker said in a news release. Walker, a Delavan-Darien graduate, attended a Nov. 5 ceremony marking the club's opening.
Thumbs up to a traveling Parker Pen exhibit. True to his word about spreading awareness of the Rock County Historical Society, Executive Director Mike Reuter has orchestrated the exhibit "Making Their mark; 125 Years of Parker Pen" at Olde Towne Mall in downtown Janesville. It helps that this first traveling exhibit is in a mall co-owned by Jackie Wood, the society's board president. Reuter said the location provides good foot traffic in a safe, secure spot. The exhibit features an interactive kiosk with touch screens that give users interactive experiences and allow them to gauge how various Parker Pen brands affected them. The exhibit, to be in place until Nov. 8, 2014, also includes company history, advertisements and Parker Pen band music. It's the type of interactive exhibit the society hopes to offer more often. In the process, the society should build its base of supporters.
Thumbs down to employers prying into private social media. Credit the state Senate for approving a bill to ban employers, landlords and colleges from requesting passwords so they can see private social media and email accounts of applicants for jobs, apartments and athletic scholarships. Some business groups applaud the legislation because it would still provide access to public Internet postings. A spokesman said Gov. Scott Walker would sign it if the Assembly passes it. Many young people today think nothing of posting online their every move—including some that might raise concerns for employers or colleges. However, letting employers, landlords and colleges pry into what applicants intended to be available online only to selected relatives and friends is almost the modern-day equivalent of demanding to read a diary. Applicants can refuse these demands but fear doing so could hurt their chances of landing jobs or being accepted. Other states have been rushing to protect such content, and it's great to see bipartisan support for this bill in Madison.
Thumbs up to Parker High's kitchen. The cafeteria kitchen in the high school on Janesville's west side scored an unheard-of 100 percent in a recent Rock County Health Department inspection. Among other things, an inspection checks for cleanliness and staff procedures, and gauges whether hot and cold foods are kept at safe temperatures. Parker's kitchen manager, Annie Lobrano, earns an "A," as does her staff. Other kitchens in the Janesville School District are near flawless, as well. Since the federal government demanded school kitchen safety and sanitation improvements in 2008, average inspection scores in Janesville's district have risen from 82 to a stellar 96.5. Jim Degan, district manager of food services, and his assistant Laurie Meehan are doing things right, as are kitchen workers throughout the district.


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BevNET Beverage School is an on-demand, video-based learning tool and online community designed for entrepreneurs and newcomers to the food and beverage industry.
Published 6x per year, BevNET Magazine is the leading industry magazine for analysis of trends, innovation, marketing, and product development.


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Short List - Content
Sailing Barge Greta
Whitstable Harbour
Contact Details
We offer barge day trips for individuals and groups from early spring through to late September/early October. You can also have private use of 'GRETA' Thames Barge by chartering the barge for the day. Our insurance allows for groups of up to 12 passengers. On Thames Barge Match Days we allow up to 8 passengers.
A Gift Voucher for a Thames Barge Sailing Trip on the 'GRETA' makes an excellent present for a birthday or any other Special Occasion.
Depending on the course set on the day by the Skipper - Steve Norris, you might see the Maunsell Forts built during World War 2 that are just over 12 miles from Herne Bay. The Kentish Flats offshore Windfarm 6.2 miles off Whitstable, the old Herne Bay Pier Head, where once steamers called to take trippers to France, The Ancient Reculver Church Tower, once more than a mile from the sea or even seals off the Isle of Sheppey!
Most of our boat trips from Whitstable are approximately six hours in duration, so bring your own picnic and refreshments. We can also arrange catering with a local supplier for large parties.
A typical day on board 'GRETA' starts 30 minutes before departure with a welcome coffee and a brief talk about the barge and safety. Slipping her moors the 'GRETA' sets out for the day's sailing. Once the sails are set and the engine is stopped a new calm atmosphere envelopes the barge and its occupants. Peace and quiet replace the stress and strain of every day life.
Lunch may be taken at anchor or underway.
If anyone in the party are of a musical ilk please bring your instruments and have a sing-a-long. Drifting along with a guitar strumming and gentle voices mingling for an idyllic afternoon.
You can just lie back and doze, wake up, eat and drink and doze again; you can help with the sails, winding the anchor or leeboards. Passenger involvement in the sailing of 'GRETA' is optional and at the Skipper's discretion.
After a day of sailing, which is to a certain extent influenced by the wind and tide, the 'GRETA' returns home to her mooring along side the South Quay inside Whitstable Harbour. For further information visit: http://www.greta1892.co.uk
Prices and opening
Please see website for more details.
Opening details
Season Dates
Opening Hours
We offer barge day trips for individuals and groups from early spring through to late September/early October. Please see the website for further details.
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Our Views: Riding the rails of good news for Rock County
When state incentives bolster a business serving other companies in Janesville and Milton, it's a victory for those communities.
When those grants and loans help reduce traffic snarls, as well, it's like a baseball team winning a doubleheader.
So it is with a package announced Friday to help the Wisconsin & Southern Railroad with two projects expected to start this fall and be completed by spring.
Motorists have complained for decades about long delays at the Five Points on the west side of Janesville's downtown. They wonder why a bridge couldn't be built to eliminate train traffic at that bottleneck.
Such a project likely would be cost prohibitive. These new state awards, however, will reduce delays at the Five Points.
The state's Freight Railroad Preservation Program granted $1.74 million to Wisconsin & Southern to build a 1.5-mile rail siding next to the track that runs between Milton Junction and Highway 14 in Janesville. The award will pay for up to 80 percent of the project. Also using a $218,040 state loan, the railroad will build the siding so it can link mile-long trains with fewer back-and-forth shifts that often block crossings in Janesville and Milton. It also will ease scheduling headaches because the railroad will be able to pull one train onto the siding while another passes.
Railroad spokesman Ken Lucht told The Gazette that the siding will "dramatically eliminate" trains idling in Janesville.
A second project has received a $2.29 million grant and $286,673 loan to reactivate a 10-mile line between Fitchburg and Oregon. That will allow Janesville-based Lycon to mine sand and gravel in Janesville and ship it to its new cement and ready-mix plant in Oregon. The village of Oregon and city of Fitchburg own that line, which hasn't operated since the 1990s. Oregon won't let Lycon run its new plant without rail service. The line will let Lycon supply cement to commercial projects in Dane County.
Reactivation of that line further boosts hopes for Rock County economic development. That's because Oregon and Fitchburg also own an extension of that line south from Oregon to Evansville. Once rail service restarts between Oregon and Madison, it should raise interest in reactivating the line to Evansville. That could create new markets for Landmark, a grain and soybean cooperative in Evansville.
"This is a major economic development step forward with implications for the entire region," state Sen. Tim Cullen of Janesville said. "Access to rail lines is a critical consideration for many manufacturers looking to relocate or expand their business, and providing greater rail infrastructure will help make our area more competitive."
Rock County residents should applaud the doubleheader victory. Reactivating the line to Evansville would extend this winning streak of economic development.


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evolution and endangered species
Discussion of everything related to the Theory of Evolution.
Moderator: BioTeam
12 posts • Page 1 of 1
evolution and endangered species
I'm a person who has always been interested in "saving endangered species", but lately while discussing with people who don't share my beliefs they throw the "evolution - survival of the fittest"-argument in my face and I don't really know what to answer to that.
Is it possible to be a believer in evolution and at the same time fight to save species that are soon to be extinct (usually because humans are hunting them/destroying their habitat etc)?
You have to keep separate your knowledge of how things work and how you want things to be. The first part is science, the second part depends on a value system. You cannot use science to prove that your value system is "correct", you can only hope that, deep down, you and the people you are discussing this with have the same value system, and then you can argue about how things work and maybe reach an agreement. If you don't share the same values, then its futile.
The polio virus is an endangered species and according to my value system, good riddance. Do you agree? Ask your friends, if their child got polio, would they shrug their shoulders and say "oh well, whatever, survival of the fittest".
So you have to ask yourself - why do I value endangered species? What is it in your value system that points you in this direction?
Personally, my value system holds human welfare above that of any other species. If saving an endangered species benefits humans, then save it, if it endangers humans, kill it, if neither, then leave it alone. I am not in favor of killing off any species unless we fully understand it, its DNA sequence, why its dangerous, and how it makes its living. We have to understand how things work in the long run, too, not just does it put money in our pockets today and to hell with tomorrow. We cannot, at this point, understand any species fully, so by exterminating it, we lose some information that we may never recover. So, unless its really dangerous or seriously impeding human welfare, save it. There's another aspect - disrespect for other species may create an environment of disrespect for "difference" and we probably don't want that.
On the other hand, we don't want to value difference for its own sake. The polio virus shouldn't be exterminated because it's different but because its bad. Respecting other species because you value diversity may create an environment of disrespect for humanity, and we probably don't want that either. I reject "diversity" and "conformity" as fundamental values, because it's dumb.
Rap, your argument is flawed. First of all, I am sure that Kajsateresa has no interest in saving poliovirus, but more interested in those on the endangered species list. Second, poliovirus infection is asymptomatic in 95% infected individuals. Thus, it probably plays a significant role is eliminated some specific human genotype. We, humans, might not like it, but elimination of this virus as well as vaccinations against it are probably doing significant damage to our (human species) genetic pool.
Ok, I may have misunderstood Kajsateresa's idea of "endangered" species, but I stick to the "philosophy". With regard to the polio virus, if you are correct and we don't fully understand it yet, then by my own reasoning or philosophy, we should be careful about erasing all traces of it.
I second everything Rap said.
The question of whether we should save endangered species is simply not a scientific one.
As for the "survival of the fittest" argument, I can think of no better response than the words of Darwin himself:
-The Descent of Man, Chapter V
Just because something is natural doesn't mean it's good.
If arguing with people on the internet helps me understand science, then I will do it. FOR THE CHILDREN.
The meanings of "Good" and "Bad" are relative. And to make the argument more interesting, consider this:
There is no Good without Bad just as there is no light without darkness.
Without contrast in the opposite, there is only sameness.
Since Good would not exist without Bad, Bad must actually be Good overall...
also agree with Rap. Why are pandas more worth saving then some bugs?
Cat: agree about the degeneration, that's basically what Luxorien quoted. So, will we save the virus and spread it around?
Cis or trans? That's what matters.
Re: Re:
This is where you really can run into a case where people with different values are talking at cross purposes. On the one hand vaccinations immediately save lives, a good thing, but on the other hand, you have increased the number of people who will have low natural immunity, in the genetic sense. Unless the wild virus, or whatever, is eliminated, you will have a steadily increasing number of people who depend on vaccination for survival. In other words, who controls the vaccine controls their lives. If the wild virus is eliminated, then who controls the virus controls their lives. Now we are into politics, and the bullshit starts flying thick and fast, left and right. I pray to God neither side wins a decisive victory, because I think it's that chaotic struggle that really generates progress, just like biological evolution.
I totally agree with you, saving endangerd species is a very important topic. We should fight to save our wildlife because it's a good chance humans are the reason they are dying.
12 posts • Page 1 of 1
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Oscar-Winner Philip Seymour Hoffman Dead at 46
Philip Seymour Hoffman, a towering icon of modern cinema, was found dead in his Manhattan apartment today according to the Wall Street Journal and New York Post. He was 46 years old.
Hoffman won a well-deserved Oscar for his work in 'Capote' and his 20-year career saw him working with many of the best and most influential filmmakers of the '00s, from Paul Thomas Anderson to the Coens. He recently appeared in 'Moneyball,' 'The Hunger Games: Catching Fire' and 'The Master,' but his extended filmography includes 'Boogie Nights,' 'The Big Lebowski,' 'Synecdoche, New York,' 'The Talented Mr. Ripley' and countless other extraordinary films. Few actors have showcased such range, with Hoffman delivering powerful dramatic performances like his role in 'Magnolia' and elevating goofy, lowbrow comedies with hilariously broad work in movies like 'Along Came Polly.' He was also a burgeoning filmmaker himself, directing the underrated 'Jack Goes Boating.'
At the time of his death, Hoffman was still working on the 'The Hunger Games: Mockingjay Part 1′ and 'The Hunger Games: Mockingjay Part 2′ and was preparing to direct Amy Adams and Jake Gyllenhaal in 'Ezekiel Moss.' The current state of these projects is undetermined.
His family has released a statement, saying:
We are devastated by the loss of our beloved Phil and appreciate the outpouring of love and support we have received from everyone. This is a tragic and sudden loss and we ask that you respect our privacy during this time of grieving. Please keep Phil in your thoughts and prayers.
While a medical examiner will determine the official cause of death, current reports indicate that Hoffman died from a drug overdose. Law enforcement officials confirm that Hoffman was found with used bags of heroin near his body and a syringe still in his arm. Considering that Hoffman checked into rehab last year as part of a lifelong struggle with addiction, this is the most tragic outcome imaginable. But no matter the circumstances, one thing is very clear: we've lost a titan of the silver screen.
Cinema is a little less beautiful and a little less powerful today.