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Problem with transgenic mice
Genetics as it applies to evolution, molecular biology, and medical aspects.
Moderator: BioTeam
3 posts • Page 1 of 1
Problem with transgenic mice
I have sent purified plasmid DNA to mouse transgenic core to develop transgenic mice with that specific DNA piece.
Out of about 45 mice that I genotyped, only about 3 mice gave me the right bands (so I have 3 founders), indicating the presence of the foreign DNA in the mouse. I got few pups from these founders that have the foreign DNA as well. However, few months later, all my genotyping results on new pups from my founders are turning out to be negative (Dont notice any bands). I was confused and re-tailed my founders and genotyped them again. Surprisingly, I dont notice the bands in my founders that I had observed previously. I really doubt that the results I saw on my founders were plain contamination (false positive results). (Note: Not all 45 mice were genotyped at once, may be about 10 mice five different times. Also, when ever I had genotyped, I had a positive control that showed positive results and negative control that showed no bands. So No contamination with my controls). I'm really confused. did any one face the same issues that I did? Also, is there a possibility that the DNA in my 3 founders got integrated in the telomere region, so as the mice got older, the foreign DNA got disintegrated. Any suggestions/answers are welcome. Thanks
Obviously I dont know.
Am I correct to believe you are genotyping by PCR? If so, the obvious answer is that your positives were false (contamination). THis is very likely if you use this gene a lot and have PCR'd before, as its likely that your pipettes are contaminated as is your PCR machine. Even if not, are you sure you did not carry over contaminants when setting up the PCR (e.g. did you add your DNA to teh tubes last, always change tips, and did you use aerosol resistant tips? ).
Presumably you still have the original DNA - this is important. Now it could be that when you went back to your founder DNA it simply failed the PCR - did you optimise the PCR, and do you have a positve control for the PCR (e.g. actin?).
Although I may be wrong on this next point, my suspicion from what you tell me is that your PCR failed but that your founders are genuine- the big question though is whether your founders have transgenic gonads - it is possible that they are mosaic - and the transgene went into the tails but not the gametes. But as your PCR is failing even though it used to work, (PCR can be temperamental...) get that working, confirm your original finding, and run PCR reactions for the offspring along with the founders everytime because depending on your gene and primers and the purity that you want to take your DNA to (phenol chloroformed EtOh pptd well diluted DNA is best), you don't want to miss the day when the PCR Gods are smiling...Good luck.
Also, from another thread post, some of these tips will be relevant:
There's loads of things you can do to optimise PCR.
From my recollection, in order of preference, if you still need help on this:
0. Do a control reaction to test all the variables (taq, dNTP's especially, to ensure the PCR worked) - (usually someone can offer you actin primers and template at known molarity).
1. Mg concentration is key - alter this, use the temperature that gave you the best result.
2. You are using cDNA - does this mean a crude RT prep? You could do a DNA clean up to remove any proteins (a 50:50 Phenol/chloroform say 100-500ul , vortex, spin 10mins, pipette of the aqueous phase to a fresh tube. dispose of phenol carefully according to local rules (evap in a chemicals fumehood is best). Ethanol precipitate the DNA using standard protocols in Mol Biol - as your target is small, use some carrier DNA to ensure you dont lose the precipate - red-pellet precipation carriers are on the market (try SigmaAldrich).
3. Use less DNA- you will probably get better results, and save your precuious DNA.
4. Use thin wall tubes for PCR - and cut your cycle hold times (means you can get faster sample to result times - great if you have insomnia anyway/ you can shorter breaks).
5. eliminate the final step of 72oC - it is rarely needed, and if you are cloning it can cause damage to the phosphate ends and your cDNA cloning might not work.
6. reduce the initial heating step time as this can damage you enzyme.
7. Avoid too much enzyme- always use a master mix of enzyme which is well vortexed to mix- otherwise glycerol in the reaction can be variable across your samples and too high. Always keep your reactions on ice. Sometimes batches of enzyme vary and have variable shelf lives...
8. Try hot-start PCR if thats not working well.
9. Consider the possibility that your larger bands are alternative spliced variants and clone and sequence them too. But beware that PCR often creates bizarre unnatural hybrid molecules which are entirely artefact.
If non of this helps, either your technique is wrong, or cut your losses and redesign your primers. I think that covers A LOT OF it!
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Public record for Sept. 27, 2013
Rock County
Intoxicated driving arrests
—LAKEISHA M. CRAINE, 27, Chicago, at 9:48 p.m. Friday, Sept. 20 at South Highway 51 and Enterprise Drive, town of Rock.
—COUNTY A AND H, TOWN OF CENTER, at 9:23 p.m. Monday. A car driven by Logan T. Dier, 25, of 830 Harding St., Janesville, was eastbound on County A when he failed to negotiated a turn, struck a mailbox, overcorrected and then collided with a car driven by Josue R. Sanchez Cortes, 20, of 2703 N. Dohs Road, Evansville. Sanchez Cortes and a passenger in his car, Jhon Sanchez Cortes, 19, of the same address, were reported transported for medical treatment. Dier was arrested on a charge of causing injury by intoxicated use of a motor vehicle. He was also ticketed for operating after revocation, failure to maintain control, speeding and operating a vehicle without insurance. Sanchez Cortes was ticketed for operating after suspension and operating a motor vehicle without insurance.

subset: commoncrawl


text:
Small trials using younger donors and elderly recipients hint that mesenchymal stem cell transfers might reduce frailty.
Smarty Genes
May 23, 2017|
Scientists have identified 40 new genes linked to human intelligence.
May 23, 2017|
Scientists have identified 40 new genes linked to human intelligence.
May 22, 2017|
Rare genetic variants could blunt efforts to destroy pest populations.
May 11, 2017|
Our guide to all known forms of RNA, from cis-NAT to vault RNA and everything in between.
May 11, 2017|
What researchers are learning as they sequence, map, and decode species' genomes
It's not only about health but also about exploring ancestry and contributing to science.
May 8, 2017|
In young mice, THC had the opposite effect.
May 1, 2017|
Author Bob Holmes dove into the taste-determining realm of his genome.
May 1, 2017|
Scientists discover transcripts from the same gene that can express both proteins and noncoding RNA.
May 1, 2017|
The green peach aphid can colonize new host plants with ease through rapid changes in gene expression.
May 1, 2017|
The Yale University cell and molecular biologist is probing the deep mysteries of epidermal cells.


text:
Welles Park
2333 W. Sunnyside Ave. Chicago, Illinois 60625
(312) 742-7511
Fee: $5
Age Range: family-friendly
Join us for the first (annual?!)Sock Hop featuring the band, Graffiti Unlimited!
Graffiti Unlimited has performed as a band for many years (two members have played together for over 30 years!). They specialize in Classic Oldies, Rock, and Blues and are donating their time to help Welles Special Recreation Parents Association raise money to subsidize the Special Recreation Program at Welles Park!
Prizes will be awarded for the best dressed and for the Hula Contest… so come prepared!
Sponsored by the Special Recreation Parents Association and Friends of the Park.
Your ticket includes a hotdog, chips, and a soda!


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Attorneys who work inside companies are often very aware of a company's culture. To understand which companies have better cultures, GMI Ratings of Portland, Maine, attempts to rate them.
The highest "Accounting and Governance Risk" (AGR) rating from GMI is Progressive Corp., followed by Range Resources, American Electric Power, CNA Financial, CSX Corp., LinkedIn, AFLAC, Under Armour, Colgate Palmolive and Whole Foods Market.
The ranking is important, and some have argued it is far more important than who the company's CEO may be.
In fact, Rakesh Khurana, a professor at Harvard Business School, said that, "large-scale statistical studies have failed to find any direct causal link between CEOs and firm performance."
The internal culture "exerts a far greater longer-term influence on the company's success," he added in a statement to MarketWatch.
Still, corporate culture is "difficult to define, much less measure," the report adds.
Yet, in trying to come up with a way to measure corporate culture, Paul Gompers of Harvard, Andrew Metrick of Yale and Joy Ishii of Stanford, said a "good proxy for corporate culture is a firm's governance," MarketWatch reports. That is defined whether it restricts "shareholder rights and thereby entrench its leadership?"
The study came up with an index which showed that over 10 years companies which had the least restrictions "gained 9.3% more than those with the most," MarketWatch said.
In coming up with ratings, GMI uses corporate governance and the reliability and accuracy of a company's financial reporting data.
Corporate culture can change. For instance, when it comes to corporate culture, InsideCounsel reported that GM shook up its corporate culture after the ignition system controversy.
"I see this as an opportunity to accelerate our culture change," GM CEO Mary Barra said in a recent statement about the events. Entrepreneur magazine has also included a report by Patrick Proctor, vice president of operations at Stash Tea Company in Portland, Ore., that a company's culture includes "values, visions, norms, working language, systems, symbols, beliefs and habits, from the inside out."
"It also embodies the internal movement, habits and overall feeling experienced by employees about the employer," the report adds. "Company culture is sometimes shared with vendors, suppliers and even customers."
In addition, a company's leadership should engage their employees to get "open, honest and creative feedback," the report said.
And the culture, as well as the company's mission and brand, work together to help the company grow over the long term, Proctor said.
For more on this topic check out these stories:
GM shakes up corporate culture in wake of ignition switch issues
General counsel must become global change agents
Globalization, talent crunch among trends affecting the legal profession


text:
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Making new wills(2 Posts)
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Eurogamer.net • 3DS iPhone PSP PlayStation Vita • App of the Day Diablo 3 Digital Foundry Lost Humanity MMO
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Public record for Oct. 31, 2013
Rock County
—�A 37-YEAR-OLD JANESVILLE MAN, at 10:59 p.m. Tuesday at the Janesville Police Department, on charges of first-degree sexual assault of a child, and battery/domestic abuse.
—�DARRIN L. HUNT, 44, of 804 Oriole Lane, Janesville, at 7:36 p.m. Oct. 29 near the corner of Holmes Street and Park Avenue, Janesville, on a charge of battery to emergency medical personnel. Hunt was riding a bicycle when he was struck by a truck turning at the intersection. When emergency workers approached, he began spitting and swinging at them, hitting one of them, police said. Hunt was admitted at Mercy Hospital and Trauma Center, Janesville, and later released.
Intoxicated driving arrests
—�WILLIAM C. SAVAGE, 39, of 2433 N. Wright Road, Janesville, at 2:35 a.m. Wednesday at Milton Avenue and Hawthorne Avenue, Janesville, after a one-vehicle accident. The arrest was listed as his third for intoxicated driving.
—�HIGHWAY 14 NEAR CARVERS ROCK ROAD, TOWN OF BRADFORD, at 2:56 p.m. Friday, Oct. 22, when a car driven by Taylor J. Hummel, 19, of W6665 Jim Drive, Elkhorn, collided with a car driven by Shannon M. James, 35, of 4209 Saratoga Drive, Janesville. James' car was hit from the rear as she slowed for a car in front of her that was turning. Hummel was transported for medical treatment. He was cited for inattentive driving.


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With the Supreme Court's historic overturn of the Defense of Marriage Act and Proposition 8 this morning, a wide array of personalities in music have joined the global celebration via Twitter. Below are some of the most colorful reactions to the ruling from industry voices:
I'm DEFINITELY going to need a 2nd job to pay for all the weddings I just got invited to. #downwithdoma #seeyouneverprop8— Tegan and Sara (@teganandsara) June 26, 2013
Gay, straight, lesbian, bi.. No one is better than any one else. What an incredible day for California AND for equality.— demetria lovato (@ddlovato) June 26, 2013
Love is love.— Michelle Williams (@RealMichelleW) June 26, 2013
DOMA unconstitutional!— Cyndi Lauper (@cyndilauper) June 26, 2013
Yeah, marriage equality!!!! On our way! #PeopleLikeUs #TieItUp— Kelly Clarkson (@kelly_clarkson) June 26, 2013
I am standing on the right side of history. I stand with @HRC for marriage equality. #SCOTUS #time4marriage http://t.co/3cSE5e0nwK— Alicia Keys (@aliciakeys) June 26, 2013
We are all equal and have the right to love. So proud to be an American. #equality— Nick Jonas (@nickjonas) June 26, 2013
#loveisloveislove Farewell #Prop8 #DOMA #lovers http://t.co/8ryfPhilkh— kylie minogue (@kylieminogue) June 26, 2013
Down w DOMA! Something to be very proud of this upcoming July 4th. We are making beautiful progress for Equality! #proud— Adam Lambert (@adamlambert) June 26, 2013
Lets hear it for the general progress of the human mind. With a beautiful sweep of sweet justice's blind hand we move forward.— Melissa Etheridge (@speak_true) June 26, 2013
We stand tall today.#DomaStruckDown So many fought for so long. Be proud, the prejudice are now the minority. pic.twitter.com/teni7GREDY— Lady Gaga (@ladygaga) June 26, 2013


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Flu season starts earlier than usual in Wisconsin
Associated Press
Thursday, November 21, 2013
MILWAUKEE — State and local health officials say the flu season is starting earlier than usual in Wisconsin.
Fifteen people have been hospitalized so far across the state. About half are in Milwaukee County. Twenty-five flu cases have been confirmed statewide.
But there's plenty of flu vaccine available,�Wisconsin Division of Public Health epidemiologist Tom Haupt told the Milwaukee Journal Sentinel.
Last year's flu season peaked in late December and was the earliest in nearly a decade. Usually, the flu season doesn't peak until late January or early February.
No flu-related hospitalizations were reported in Wisconsin before January during the 2010 and 2011 seasons.


text:
Oxygen XML Developer is an Oxygen distribution specially tuned for XML development, providing XML editing, XML conversion, XML Schema development, XSLT/ XQuery/ XPath execution and debugging, SOAP and WSDL testing, Native XML and relational database support, and XML instance generation.
Xidel is a command line tool to download Web pages and extract data from them. It can download files over HTTP/S connections, follow redirections, links, or extracted values, and process local files. The data can be extracted using XPath 2.0, XQuery 1.0, and JSONiq expressions, CSS 3 selectors, and custom, pattern-matching templates that are like an annotated version of the processed page. The extracted values can then be exported as plain text/XML/HTML/JSON, or assigned to variables to be used in other extract expressions or be exported to the shell. There is also an online CGI service for testing.
Snippetory is a general Java template engine based on passive templates. In passive templates, template code and logic are clearly separated from each other. The templates contains very simple mark up. By removing logic, templates are directly accessible, fully parametrized, and free of context, and can easily be reused. Navigation, code competition, and structuring of the code with methods and classes are supported.
pdirl (PHP Directory Listing) is a tiny directory lister program. It is meant to be better looking and more useful than the directory listings produced by Apache. It features searching, directory listing in subdirectories, breadcrumb navigation, translations, and MIME-type detection. It requires PHP 5.
Vis/Space is a client-server system for visually exploring data structures in 3D virtual space. It allows the placement of various data sources in a three-dimensional environment. Custom search terms can be entered into a query field, or can be navigated to and from an existing object. All objects like images and "text screens" can be moved around. A single click on an image shows the full-resolution version in the foreground. Whole collections of images can be moved. A click to the cube also toggles among several different possible layouts. Data sources include search engines, microblogging, Wikipedia, image/video search, and 4chan.
PHPTAL is a fast, safe, XML-based template engine for XHTML and HTML5. It implements Zope's Template Attribute Language, which has clean, concise syntax that is compatible with XML tools (no custom ugly tags), and template source files can even be previewed in Web browsers or opened in WYSIWYG editors. Templates are compiled to PHP and cached, giving great performance and taking advantage of PHP accelerators. PHPTAL by default ensures well-formed output and escapes XML-unsafe characters everywhere, which means that your pages can be completely safe against HTML-injection (XSS) without great effort. It features fine-grained caching of output, pre- and post- filters, internationalization via gettext or custom backend, creation of custom template attributes and expressions, and templates loaded from non-standard sources (e.g. databases). It supports UTF-8 well, is easy to integrate and redistribute with PHP frameworks and applications, and has an object-oriented design and a comprehensive unit test suite.
seltz_analyzer is a PHP class that tries to find the most important words inside a well-formed XHTML trunk. Every word takes a score based on the role in the XHTML structure. For example, a word between strong tags will take 5 points. In addition, it will look at some simple syntax rules. For example a word with the first character uppercase will take 4 points. The score is cumulative, so the more a word is used, the more meaning it will have.
An extended "make" replacement that maintains/updates/regenerates programs.
A server operating system with an integrated identity and infrastructure management system.


text:
Black Point hosts 'Death and Dying'
LAKE GENEVA — This time of year, people can't seem to get enough of goblins, ghosts and zombies. The staff at Black Point Estate, Lake Geneva's historical site and unlikely candidate for such things, is hoping to tap into the lure of Halloween tales of the dead and undead with a special Oct. 18 program, "Death and Dying in the Victorian Era."
Read the current edition here: http://www.server-jbmultimedia.net/CSI-WalworthCountySunday
Estate Director David Desimone explained, "One of the greatest challenges for historic house museums is creating new and different programs for repeat visitors. While this program is building upon the rise in popularity surrounding Halloween, we will remain true to our mission of sharing historically accurate stories."
That means there will be no zombies lurking on Black Point's wrap-around front porch, no ghosts drifting up the stairs, no goblins looking for treats in the parlor and no headless horseman galloping up the hundred steps from the lake. Before you get too comfy, though, you should know there will be plenty of tales of woe and misery, laced with a good amount of funeral history and lore.
Read the complete story HERE.


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Rock County unemployment rate falls in March
MADISON—The unemployment rate in the Janesville metro area dropped in March, the Department of Workforce Development reported Wednesday.
Statewide, unemployment rates fell in all 12 metro areas.
The department said the March rate for Rock County—also known as the Janesville Metropolitan Statistical Area—was 7.8 percent, lower than the 8.1 percent reported in February and the 8.8 percent tallied in March 2013.
Overall, the state unemployment rate in March was 6.7 percent, down from the 7 percent rate posted in February and lower than the 7.5 percent posted in March 2013, the department reported.
Of the 32 Wisconsin cities with at least 25,000 people, unemployment rates decreased in all but three.
Among counties, Rock County had the 29th-highest rate, while Dane County had the lowest at 4.4 percent.
Walworth County had a March unemployment rate of 7.2 percent, while Green and Jefferson counties had rates of 6.3 percent and 7.3 percent, respectively. February-March rates decreased or remained the same in all but six of Wisconsin's counties.
Among individual cities, Janesville's rate of 8 percent in March was the 10th-highest in the state.
With a March unemployment rate of 10.3 percent, Beloit had the second-highest rate among Wisconsin cities. It trailed only Racine and its March unemployment rate of 11.8 percent.
Local unemployment rates are not seasonally adjusted.


text:
Two Janesville residents arrested on marijuana-sales charges
JANESVILLE—Police with a search warrant arrested two Janesville residents Monday on charges of marijuana trafficking.
Officers came to the door of 715 Kellogg Ave., No. 1, at about 1:15 p.m. Monday. The search warrant stemmed from an investigation into allegations of marijuana sales at the residence, according to a news release issued Thursday.
Police said they seized 4.5 ounces of marijuana, drug paraphernalia related to the packaging and sale of marijuana and $116.
Two small children living at the residence were placed with Rock County Child Protective Services.
Two residents of the apartment, Crystal M. Baumann, 33, and Christopher J. Baumann, 32, were arrested on charges of delivery of marijuana and running a drug house, police said.
Christopher also was arrested on charges of possession with the intent to deliver marijuana and possession of drug paraphernalia.
Last updated: 5:32 pm Thursday, September 26, 2013


text:
The Fiber Disease
Human Anatomy, Physiology, and Medicine. Anything human!
Moderator: BioTeam
Olive Leaf Extract helps.
As London points out, Ellagic acid is also very helpful. I've been taking it for years-
Tam Tam,
Thanks for the new video. Some things make more sense.
I had to give up the search on your post- too much info- It is very interesting though. You may have a key there....
Last edited by J Jill on Sun Oct 29, 2006 8:55 pm, edited 2 times in total.
"When you dine with the devil, bring a long spoon."
Many dried food-products are rehydrated before consuming. Examples are pasta, legumes, potato-
powder for puree, dried milk, instant products (coffee, chocolate, cocoa, sauces), puddings
and other desserts. The rehydration is possibly combined with dissolution, swelling or
gelling after sufficient mixing, homogenisation, heating or cooling. Duce and Hall (1995) visualised
in various foods (like butter bean, rigatoni and a snack food) the hydration and rehydration
together with the subsequently induced structural changes.
As mentioned above in section (b) the structure of a tablet determines the kinetics of the dissolving
of the tablet (Snaar et al. 1998, 2001) and thus the drug release (Korsmeyer et al.
1985, 1986; Rajabi-Siahboomi et al. 1994; Bowtell et al. 1994; Mäder et al. 1997; Black et al.
1998; Fahie et al. 1998). Tablets with hydrophilic matrices are dissolved in gastric juice and
saliva of patients. This process was simulated by dissolving a tablet in a 0.5 mol HCl solution
(Groß and Lehmann 1998; Fig. 3.1.6). Liquid diffuses into the tablet matrix, polymers present
in the matrix hydrate and eventually build up a gel layer. This layer acts as a barrier for further
liquid ingress and, thus, for the drug release. Depending on the matrix-composition the tablet
swells. MRI shows the zones of the solid core, the gel and the outside liquid. In coated tablets
the coating can also be observed as a further zone. The evolution of the drug release is controlled
by the polymer (acting as gelling agent or increasing the viscosity and thus diminishing
the mobility of water in the tablet) and the structure and composition of the matrix (hydration,
swelling and crack behaviour).
In order to record the migration of brine into fresh meat 23Na was imaged (Guiheneuf et al.
1997). The uptake of brine is a good approximation of a Fickian diffusion process with an
Na+-concentration-dependent diffusion coefficient. Blackband and Mansfield (1986) used
MRI to study the diffusion of water into polyamide. Polyamide is often used as an oxygenbarrier
in plastic packages, but only, if the water content in the polyamide is zero. Gil et al.
(2000) observed the imbibition of water in differently prepared types of cork. Suberin is quantitatively
the main component in cork of cork oaks (Quercus suber L.) and is believed to be a
- 31 -
biopolyester. Suberin seems to be principally responsible for slowing down the uptake of water.
Webb and Hall (1991) and Gibbs and Hall (1996) showed that the ingress of some organic
fluids used as model-petrol into vulcanised rubber is a Fickian process. In superabsorbing polymers
local differences in the cross-link density (T2) can be detected (Traub et al. 2000). T2 is
longer in the centre than at the outside, this means the minimum of the cross-link density is in
the centre. Besides the water uptake the swelling process can also be monitored as a function
of time. Prado et al. (1999) studied the water uptake of water in pellets of zeolite using SPI.
This was necessary due to the small T2-relaxation times of water in micro-porous materials.
Zeolites are used in separation processes, as catalysts and drying agents.
Bohris et al. (1998) observed three types of water in Portland cement pastes during curing.
These are chemically bound, gel and capillary water. The effect of the water to cement ratio
and of the cure conditions on the water migration and the distribution of the three phases during
curing is discussed.
a b c
Fig. 3.1.6. Serial MRI (interval between two images: 1 h) of the dissolving of a tablet in a
0.5 mol HCl solution (Groß and Lehmann 1998). Measuring sequence: Spin Echo
3D with the sequence-parameters: FOV = 15x15x15 mm; number of pixels
N = 128x128x16; A = 117·117 µm/pixel in plane and 9375 µm slice thickness;
TE = 2 ms; TR = 200 ms; averages: 1, total measuring time of a single experiment:
7 min, time a: 7 min; time b: 10 h 7 min; time c: 17 h 7 min; 300 MHz
In many polymers cross-linking is observed after the extrusion and the die-casting. The mobility
of specific segments correlates with the cross-link density (Fülber 1996; Blümich 2000;
Götz et al. 2002a, b). This has a strong influence on the T2-relaxation. Thus the evolution of
the polymerisation/vulcanisation can be observed by T2-weighted MRI methods. Prado et al.
(2000) used SPI to localise the vulcanised boundary in rubber. Jackson et al. (1991) and Jezzard
et al. (1992) studied the polymerisation of methylmethacrylate to polymethylmethacrylate
in a mould which is temperature-controlled.
As described in chapter 2.1 the main motivation of this work is to demonstrate how NMR and
MRI can be used for a better understanding of technical or natural processes. In drying technology
for example it is assumed that a reliable prediction of the temporal evolution of the drying
is impossible for real products (Gnielinski 1998). In order to find suitable dryers for a certain
product, practical drying experiments still cannot be forgone. The main reason is that the
distribution of water or other fluids, which should be removed, and - in combination - the character
of the mass and heat transport cannot be derived from the total vaporised amount of fluid
(Harding et al. 1997; Gnielinski 1898). That means that the knowledge of the evolving concentration
distribution (or distributions) is required to characterise the drying process, to derive
relevant material or system parameters for theoretical or numerical calculations of the
drying process or for the development or improvement of existing constitutive equations. Examples
of combinations between NMR results and modelling are already given for freezing
(Kerr et al. 1996), rehydration (Hills et al. 1996a), characterising of pore systems."
the above work is on loan from:
"Applications of NMR to Food and Model Systems in
Process Engineering
Dr.-Ing. Joachim Götz
Vollständiger Abdruck der am Wissenschaftszentrum Weihenstephan der
Technischen Universität München zur Erlangung des akademischen Grades
eines Doktor-Ingenieurs angenommenen Habilitation
Januar 2004 (eingereicht Mai 2003) "
Hi guys,
I just find it strange as hell that a lot of people are sure coming up missing lately, only to be found (some anyway) later with amnesia.
So, I put the word out today about this(not publically but to my sig. others) and hope some of you that are not on this
side of evil will do the same. I will also contact the police and news media tonight. This is scarey and it's not right. We do have people following some of us. Do you actually think some of us are already as f-ing STUPID as you are trying to make us.????????????
Look f-ers, you want me to go to the news media (yeah I know you own most of them, but you sure the f. don't own all of them., Also, this man that is suddenly missing, is a teacher and coach. HMMMMMM>>>>>>>>>>>>>
And, AT & T, you quite frankly suck rocks. I'm dropping your sorry arses. Did you know that while you are recording my calls, I'm recording who is recording me? HUH, did you know that? Also, dunmbf_ _ _ _, I just loved your stupid arse survey on the top news stories of todays page.......
(for any of you that missed it), it showed this girl lying on this bright orange couch with her silver coated laptop. (Poor girl does not know she is about to get f-up....)
You know f-ers, this sucks. I was trying to play fair, but it ain't happening. You will get in the biggest heap of trouble when/if I go missing. I've alerted a few people today
and no, I did not use my cell phone, nor email to do this.....now quit following me and quit making things that are contaminated. Hell, I'm rather scared to even shower tonight, b/c the water co, was out here today doing a faux inspection.....yeah, right! I hope my new filter works okay. I got the expensive kind with the carbon filter that has to be changed.
Also, I have not spoke about what else I found in my house. That's for another time.
Look, I ain't going to Mars with the rest of the tards, got it, I ain't going////and if I do get put on that wacky ship , you and yo mamma will be on it too. F.sticks, you suck rocks.
Is this what happened to chandra Leevey? HUH??????
What about Monica and Bill? Was she a drone? You people are sick.
I hope to God someone replies to this really fast because of a few reasons. One b/c I know some names of Co's that are behind this. Your legal staff missed something too. It does have a hole that they did not catch. I have asked for help but got none. You know how to inactivate this. I know you do. Well, if you gotta do what you gotta do, then you give me no choice. Time is ticking. I did not ask for much, so hopefully we can reach some type of workable deal. OR NOT, your call. Free me or your going to go down with me.
I hope you see this. You have been around on these boards a lot longer than I. If perhaps you are friends with someone "IN THE KNOW" or maybe that your friend has a contact point with someone they know, to
please do so and have them read my post, maybe try to reach me.
I find it funny that the warner cable people were here 3 days ago, the phone co's the next, the pool man>>>>>always at night, which is strange to be here b/w the hours of 10pm -12 am, then the water co, was here today supposedly doing it;s annual inspection????what, in the boiler room?
What's going on, you are recording me thru my tv aren't you? Your not going to get away with this. JJill, this part was not directed at you.
I want my tax money back.....all you people do with it is go to oklahoma state and build some lightweight air planes that prob. defie gravity, then build more satelites up in space to spy on us.
The friggin eye-maze trick that I was being given is ont that was modeled after an old antiquated prison in ireland.
It used the eyes to get this virus into us. just like these lil green footballs try to do to me when I see them.
Last edited by London on Tue Oct 24, 2006 4:25 am, edited 1 time in total.
Okay then, I have contacted my local news departments. I have survellence too. I have the numbers where my cell calls are routed too.
Thanks att and t; you still suck rocks. Now, on to the newest member in our diatribe..........
It's dupont. It's a fact Jack.
PS: I'm waiting for some help. email me the solution to my HEALTH PROBLEM, this all goes the f. away and it will never return. Looks like you have nothing to gain this time but a hell of a lot to lose. do you not think that maybe some of us are very good at investigational work? Do you not think that we can hire help? look, I know how powerful you guys are, but maybe this will let you know my power too. No where near yours but I STILL OUTSMARTED YOU, DID I NOT?
and to be really honest and way to nice to you......this is to let you know that I did not tell the new depts your names but that a lot of people that are turning up with amnesia and also missing is probably related to Morgellons. so there, no biggy.
okay, my friend (the one that has the Father that knows the Duponts/ house in telluride ring a bell?) is on the way over here.
He is going to take it from here and tell me who, what I should do. Hell, maybe his Father will make a call or two? Hope so.....so, if any of you would like to PM me, c'mon, the sooner the better. I'm dead serious.
Okay, so like "No one" even wants to know the name of the Co.? The one that makes the honeycomb like fibers? The ones that don't get softer in the water? I thought it was strange the first time I heard
Cliff M. speak on the radio and pronounced Morgellons like "Magellans" >with the g sounding like a j sound. Then, I noticed it in a couple of other people too that knew also the correct pronunciation. Now, wonder why this is?
Look at this list. This "Maxwell Johnson" with OEM software sent me an email on my "bulk" messages dept. and tried to f.up my computer once again. Yeah, Mr Johnson, put this thing on my comouter where it would not let me erase my bulk spam unless I did it one by one. If I checked
the spot that said "check all messages" then clear, nothing would happen.
So I cleared them one by one until I got to the very last one which was
the one from OEM software then I sure the hell was not going to open it stupid so I checked the box that said "check all here" then I hit clear. Well, this is when I noticed that when I checked the box by his name that it also would check that lil box above it that said sender (THE ONE THAT HAS NEVER, EVER HAD A BOX BY IT BEFORE.)
sO, mR. jOHNSON, SINCE YOU TOOK THE FIRST LICK, IT'S MY TURN.
nOW, mR. jOHNSON, i NEED SOME FRIGGIN HELP. yOU ALL NEED TO COOL IT. aS i SAID, GET ME WELL, THIS ALL GOES AWAY. THREATEN ME, FOLLOW ME ONE MORE TIME W/ YOUR SHINY S CARS (AND i JUST LOVED THE ONE W. THE HUGE SMILEY FACE ON IT TODAY.)
aNYWAY, DO NOT COME IN MY HOUSE AGAIN. jUST LIKE LAST SUMMER
AND YOUR REVERSE OSMOSIS TRICK. i WILL HAVE SOMEONE GARDING ME W/I N THE NEXT 12 HOURS. oF COURSE YOU DID NOT KNOW THIS, BUT i HAVE VIDEO OF WHAT YOU PUT OUT INTO MY HOME...
Are you ok?
You don't know me - but I've seen your posts on several of the forum's including Lymebusters. I was reading thru just now and am concerned about you - are you alright?? The posts you've made have me concerned for you?? Is there anything I can do to help - although I am no where near you??? I guess I just want you to know that yes - someone is out here and can hear what you are saying???
Whadda you know? We made the New York Times!
What kind of investigative reporting is this? They did not even research Travis's death properly.
http://www.nytimes.com/2006/10/24/healt ... ref=slogin
Greema posted this on LB so I thought I would pass the info on. Should be a great show!
THREE HOUR SPECIAL MORGELLONS BROADCAST #5 RENSE.com
Tuesday Evening, October 24, 2006,
7:00 PM to 10:00 PM PACIFIC TIME
For Morgellons Datafiles go to:
( http://www.rense.com...t.htm )
GO TO: http://www.rense.com/
You can subscribe to the streaming audio by the month OR
click on "Listen Free" for tonight's show.
If you need a free link to the broadcast tune in to radio link early by 6:30 to get connected. Links are limited and will be gone by air time.
Together we are working in an attempt to unravel the mysteries of this HORRID affliction.
Please have a video camera always ready and document anything that you feel threatned by. Without this kind of proof you may be a target in more ways than one. Good luck.
Last edited by Sabrina on Tue Oct 24, 2006 1:40 pm, edited 1 time in total.
http://www.pubmedcentral.nih.gov/articl ... rtid=30211
"The repression of lasI by QscR could serve to ensure that quorum-sensing-controlled genes are not activated in environments where they are not useful."
QUOROM SENSING CONTROLLED GENES????? Can genes quorum sense?
Ok, I answered my own question, I think. It is the genes that enable this mechanism to happen in the first place, right?
Last edited by Sabrina on Tue Oct 24, 2006 1:45 pm, edited 1 time in total.
While quorum sensing is a well-established mode of intercellular communication in bacteria, its role in fungal systems is still emerging. Chen and Fink found S. cerevisiae use aromatic alcohols as signals to stimulate filamentous growth in response to nitrogen starvation.
Interestingly, they said those molecules elicited different effects in the yeast strain Candida albicans, suggesting the newly identified fungal quorum sensing signals are species-specific.
QUORUM SENSING FUNGUS???? Can fungus quorum sense too???
Sabrina, all,
I just googled "morgellons" and first link was for a new book coming out--wonder if it is a ruse?
"Morgellons: Mystery Disease: Delusion, or the Next Global Pandemic?" by Dr. Susan Nickerson, DCPT. Anyone ever heard of it/her?She is listed a allopathic dr.
thanks, as always,
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