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Olivia Burton Ladies' The Dandy Watch
This ladies Olivia Burton The Dandy watch is made from PVD gold plated and is powered by a quartz movement. It is fastened with a black leather strap and has a white dial. The watch also has a date function.
This watch is supplied with:
- - Official Olivia Burton presentation packaging
- - Official Olivia Burton guarantee
- - Instruction manual (where applicable)
- - 76 Reward points
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Rating Breakdown (Average rating: 5 / 5)
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Rating: Excellent (5 / 5)
Bought this for my girlfriend- she absolutely loves it! I got her the one she asked for and this watch she preferred this one. It has a supple, slim leather strap and an elegant face to it. Stylish look- the wrap around is quite unusual but neatly crafted. My girlfriend researched the brand- its two fairly new London fashion designer upstarts which makes the piece more unique.
Rating: Excellent (5 / 5)
Excellent product, I bought it for my girlfriend and she simply loved it. Classic style but at the same time very modern and extremely stylish, perfect for every occasions!
Rating: Excellent (5 / 5)
The Dandy Watch is lovely, with its minimalistic design, it is a watch which looks different, but is also very practical - you can wear this watch with anything, and it will still suit the occasion. At about 28mm, the watch face is quite petite, which is good if you have relatively small wrists and the simple wrap-around strap adds a level of fashion to it, but as it is a simple leather strap, does not overly dress-up the watch. It is light, and the watch and the strap feels well made. The watch comes in a well designed box, and an instruction manual, although it was not needed.
Several things to note - the gold is not a bright yellow gold, but is much paler. In addition, the watch is 10mm thick which is the same as a Seiko kinetic watch that I own!, with the apparent thickness increasing when you wear it due to the doubling over of the strap - this was not something that I appreciated from the photographs on the website, and is quite thick for a simple quartz watch, but otherwise I am very happy with it.
Service from WatchShop was excellent, with swift service delivery within 48 hours of ordering and good packaging protective, but also easy to open.
Overall, this was an excellent purchase, and would recommend this watch to anyone looking for a classy, but different timepiece!
Rating: Excellent (5 / 5)
The product looks nice but in my opinion the nails on the hand model are too long and off-putting.
Other independent review sources for this product
The Olivia Burton watch collection has gained a cult following thanks its vintage-inspired designs. Olivia Burton watches are available with a small 'midi dial' or large 'big dial'. This variety in size means watch-lovers can choose between a statement piece that makes an impact, and a small watch that adds subtle detailing to an outfit. At first glance, the range of watches may appear simplistic, but thanks to Olivia Burton's obsessive attention to detail, they're anything but. The simpler watches feature soft metallic hues coupled with pearlescent faces for a glossy finish. Statement designs showcase a pure love with all things traditionally British. The countryside is beautifully represented with bold images of fauna, while British classic Alice in Wonderland is used to inspire a fantastically stunning range. Lovers of vintage and British style should look no further than the Olivia Burton collection.
Watch Shop is an authorised Olivia Burton stockist.
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My name is Dalibor Pavlovic, I'm male and 34 Years old. My Sign of Zodiac is sagittarius. I live in Trstenik, Serbia. On Flirtic.com I've been registered for 06.12.2012 years.
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Eastern Orthodox miracles falsify the Catholic Church's claim to be the only One, Holy, Catholic, and Apostolic Church founded by Jesus Christ
2. There are sometimes genuine miracles outside of the visible confines of the Church. The only true miracles of this sort are worked by and through persons of good faith (e.g., St. Sergius of Radonezh){3}--as opposed to loud lifetime formal schismatics and heretics of whom there is no record of their entrance into the Church or desire therefor before their death--and in support of Catholic truth that the separated churches have maintained to varying degrees.{4} There are certain Eastern Orthodox individuals, who in the Church's words, despite being "outside the visible boundaries of the Church," nonetheless retain an "imperfect communion with the Catholic Church" because they value and practice "the written Word of God; the life of grace; faith, hope, and charity, with other interior gifts of the Holy Spirit, as well as visible elements, all of these coming from Christ and leading back to Him."{5} On 10/1/2009 I discovered that Fr. Martin Jugie reasons in the same basic way when he discusses the manifestation of fruits of holiness in the Eastern Orthodox Church.{6}
3. God never works miracles that justify and sanctify error/heresy and schism.{7} Thus, any Orthodox "miracles" of this breed are not real miracles by God, but the product of (1) demons; (2) imagination; (3) hallucination; (4) exaggeration; (5) legendary accretion; or (6) outright fabrication.{8}
4. There have been many true miracles in support of doctrines that the Orthodox have (but not via an ecumenical council) forsaken: e.g., Eucharistic miracles with unleavened as opposed to leavened bread,{9} God endorsing the theology of St. Thomas Aquinas,{10} and the Θεοτόκος proclaiming her Immaculate Conception to St. Bernadette Soubirous.{11}
Notes & References
{2} Huysman, Will R. "Why God Led Me to Rome Instead of Constantinople." The Banana Republican. 31 July 2009. 21 Sept. 2009 <http://thebananarepublican.blogspot.com/2009/07/why-god-led-me-to-rome-instead-of.html>.
{3} St. Sergius the Wonderworker of Radonezh (Сергий Радонежский, b. Bartholomew: abbot) [5/3/1314-9/25/1392] is officially a saint of the Catholic Church. St. Sergius of Radonezh is on the Roman Martyrology for 9/25. For documentation of official sanction of other post-1054 Orthodox saints, see my post "Post-1054 Common Saints."
{4} Personal communication with Mr. James Likoudis. Cf. Jugie 448, referenced below in n. 6.
{5} UR (Unitatis redintegratio) 3. Cf. In Dominus Iesus, His Holiness Pope Benedict XVI (then Cardinal Ratzinger) says, "For those who are not formally and visibly members of the Church, 'salvation in Christ is accessible by virtue of a grace which, while having a mysterious relationship to the Church, does not make them formally part of the Church, but enlightens them in a way which is accommodated to their spiritual and material situation. This grace comes from Christ; it is the result of His sacrifice and is communicated by the Holy Spirit;'81 it has a relationship with the Church, which 'according to the plan of the Father, has her origin in the mission of the Son and the Holy Spirit.'82
(81) JOHN PAUL II, Encyclical Letter Redemptoris missio, 10.
(82) SECOND VATICAN COUNCIL, Decree Ad gentes, 2. The famous formula extra Ecclesiam nullus omnino salvatur is to be interpreted in this sense (cf. FOURTH LATERAN COUNCIL, Cap. 1. De fide catholica: DS 802). Cf. also the Letter of the Holy Office to the Archbishop of Boston: DS 3866-3872."
{6} See especially p. 448 of Jugie, Martin, A.A. Le Schisme Byzantin: Aperçu historique et doctrinal. Paris: P. Lethielleux, 1941.
{7} Aquinas, St. Thomas (Angelic Doctor). Summa Theologica II-II, q. 178, art. 2, ad 3.
{8} The Catholic Church has the Congregation for the Causes of Saints to rule out cases that belong to one or more of those categories. Historically, the Catholic Church has been much more rigorous than the Orthodox Church in this regard (Jugie 450).
{9} Huysman, Will. "List of Catholic Miracles." The Banana Republican. 30 July 2008. 21 Sept. 2009 <http://thebananarepublican.blogspot.com/2008/07/list-of-catholic-miracles.html>. On the Eastern Orthodox dogmatic rejection of the use of unleavened bread, see the following: Two Epistles of Patriarch Michael I Cerularios of Constantinople to Patriarch Peter III of Antioch; Encyclical of Metropolitan Mark Eugenikos of Ephesus (1440); Council of Constantinople with Patriarchs Jeremiah II Tranos of Constantinople, Silvester of Alexandria, and Sophronios IV of Jerusalem (1583); Encyclical of the Eastern Patriarchs Anthimos VI of Constantinople, Hierotheos II of Alexandria, Methodios of Antioch, and Cyril II of Jerusalem to Pope Pius IX of Rome (1848); Encyclical of Patriarch Anthimos VII of Constantinople to Pope Leo XIII of Rome (1895).
{10} Kennedy, Daniel. "St. Thomas Aquinas." The Catholic Encyclopedia. Vol. 14. New York: Robert Appleton Company, 1912. 21 Sept. 2009 <http://www.newadvent.org/cathen/14663b.htm>.
{11} "Biography of Bernadette Soubirous." Biography Online. 2 Oct. 2009 <http://www.biographyonline.net/spiritual/bernadette-soubirious.html>.


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Holzindustrie Schweighofer (HS)
Status: Disassociated
Complainant: WWF Germany
Defendant: Holzindustrie Schweighofer (HS)
Filed: 6 November 2015
Accepted: 24 November 2015
Complaints panel report submitted: 14 October 2016
Country: Romania
Allegations: The complainant alleges that HS is involved in the purchasing and trading of illegally harvested timber in Romania and that this is having a negative impact on the country's natural protected areas.
|03-04 July 2017
FSC second Stakeholder Working Group meeting for the revision of the FSC Conditions Framework (consultation draft).
|19 June 2017
FSC is providing two webinars in English and Romanian about the process for the development of the FSC Conditions Framework. See here.
|23 May 2017
FSC lauches 30 day public consultation of the FSC Conditions Framework (consultation draft) developed by the Stakeholder Working Group.
Please see the consultation page here.
|04 May 2017
FSC first stakeholder engagement meeting for the development of a roadmap process. For more information please see here.
|28 March 2017
FSC enters into a dialogue with the Schweighofer Group in view of developing a roadmap towards ending the disassociation. For more information please see here
|20-21 Mar 2017
|First meeting of the investigation team in Bonn.
|15 Mar 2017
|Initiation of the Policy for Association investigation of the additional information presented to FSC International. For more information see here.
|17 Feb 2017
|FSC disassociation from the Schweighofer Group is made public on FSC website.
|14 Feb 2017
|FSC Director General Kim Carstensen informs WWF about the decision to disassociate.
|13 Feb 2017
|FSC Director General Kim Carstensen informs the Schweighofer Group about the decision to disassociate.
|09 Feb 2017
|FSC BoD decides for FSC to disassociate from the Schweighofer Group, and establishes a set of conditions that Schweighofer shall fulfill in order to end the disassociation.
For more information about the rationale on the decision see here
|07 Dec 2016
|FSC publishes its decision to place the Schweighofer Group on 'probation' for possible disassociation until the FSC BoD meeting in February 2017.
For details on the rationale for this decision see here.
For details about the conditions that need to be fulfilled by the Schweighofer Group in order to remain associated with FSC see here.
|28 Nov 2016
|FSC Director General Kim Carstensen informs the parties to the complaint about the decision taken by the FSC Board of Directors
|14-18 Nov 2016
|The FSC Board of Directors takes a decision about the case during its meeting held at Brazzaville (Republic of the Congo)
|14-30 Oct 2016
|The FSC Quality Assurance Unit produces a quality assessment report, assessing the quality of the complaints panel evaluation report
|14 Oct 2016
|Submission of the complaints panel evaluation report with a recommendation to the FSC Board of Directors
|20-21 Apr 2016
|First complaints panel meeting in Bonn
|18 Apr 2016
|Start of the complaint evaluation
|7 Mar 2016
|Establishment of a complaints panel
|24 Nov 2015
|FSC accepts the complaint filed by WWF Germany
|6 Nov 2015
|WWF Germany files a complaint with FSC International against HS
Use the links below to access additional information about this case:
- Case summary
- FSC news item
- FSC statement
- Frequently asked questions (FAQs) about FSC Policy for Association complaints
- Frequently asked questions (FAQs) about the Schweighofer case
- Frequently asked questions (FAQs) about the February BoD decision
- Rationale for decision of Probation_additional Q&A
- Public version of the Complaints Panel Evaluation Report
- Processing Policy for Association Complaints in the FSC Certification Scheme (FSC-PRO-01-009)
- FSC Stakeholder Engagement Plan for Schweighofer roadmap
- FSC roadmap process for Schweighofer_12 May 2017
- FSC Stakeholder Engagement Plan_FSC roadmap for Schweighofer_April 2017
- FSC enters into a roadmap process with Schweighofer_March 2017
- FSC disassociation from Schweighofer Group_FAQs_2017-03-30
- FSC statement_Schweighofer Group_FINAL_Feb 2017
- FSC disassociation from Schweighofer Group_FAQs_Feb 2017
- FSC Statement_7 December 2016_Schweighofer
- FAQs_Schweighofer case_7 December 2016
- Rationale for probation Schweighofer Dec 2016
- 160623_FSC Statement_Schweighofer certificate suspended_FINAL_EN


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Trevor Nelson at KOKO
The pioneering British DJ and Radio One presenter takes his Club Classics tour around the UK, delivering his trademark array of old school soul classics, modern R&B hits and forgotten gems from the 1970s to the present day. This London date marks the end of the tour and Nelson an incredible line-up of guests to ensure the party closes with a bang.
Venue Details & Map
KOKO London
- Address
1A Camden High Street
CamdenNW1 7JE
- Telephone:
- 0870 4325527


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The Storm Rascal 1 Ball Roller is an ideal bag for transporting a single bowling ball, accessories and a pair of shoes without the strain on your shoulder or arm. This rolling bag has easy to open compartments due to its large oval bolt zippers and is durable due to its reinforced stitching. The foam base provides support and cushion that will hold protect your bowling ball.
Thanks for asking your question! Once approved by our staff it will be posted here for others to answer. If you'd like to receive answers to your question via email enter your email address below.


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Design plus words, letters, and/or numbers
Advertising, marketing and promoting tourism in Collier County, Florida
Business in Naples, FL


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'Refreshed' autism strategy is all too stale, say activists
Government plans to "refresh" England's autism strategy will do nothing to address its "fundamental flaws", according to autistic rights campaigners.
The Liberal Democrat care services minister Norman Lamb launched Think Autism, the "refreshed" strategy for adults with autism, at Saracens rugby club in north London this week, but activists are angry that he announced no major improvements.
Lamb said there was a "new focus on building supportive communities, promoting innovative local ideas and services and improving advice and information for people with autism".


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Cammie Ferreras
Listed by Bhhs Verani Londonderry
At last, the 1 Level home you have been looking for! This well kept 3 bedroom Ranch Style Home is in beautiful condition. It features hardwood floors, new windows, Central AC, Generator hook-up and an updated kitchen with granite counters, newer appliances, and recessed lights. Enjoy a maintenance free exterior with vinyl siding, and 2 zone irrigation. Great level back yard with patio area for entertaining outside and its fenced too. Detached 24x26 Garage sized for high vehicles, and plenty of space in the driveway for more parking. There's even room to expand in the dry lower level if you need more space. Pride of ownership is evident throughout this home. Why not move right in and enjoy some easy living!
Date Showings Begin:
Delayed Showing: No


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SEO in 2015 has become something of a minefield. Many agencies lay claim to fame for 'silver bullets' and 'holy grails' and 'guaranteed rankings'. The problem is that no-one, Read More
Do you feel you are you losing online revenues because of abandoned shopping cart sales issues on your website? Below are 5 tips on how you can decrease shopping cart Read More
Online reviews and ratings (RR) on products and services for your business has never been more important than now. Search engines Read More


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The United Progressive Alliance (UPA) government's effort to erase its past failures with the auction of 2G telecom spectrum licenses that were cancelled by the Supreme Court due to allegations of corruption in the allotment process has turned out to be a flop. But its failure to spark a bidding war between industry giants should not be confused with proof that there was nothing wrong with the first-come, first-served system used in 2008, argues FirstPost.in's R. Jagannathan.
As GlobalPost reported earlier, the government supposedly hoped to generate some $7.5 billion in revenue from the auction, but the bidders had other things in mind.
But the auction doesn't vindicate former telecom minister A. Raja, who is accused of taking kickbacks in exchange for bumping certain firms to the head of the line in the "first come, first served" allotment of licenses -- which has nothing to do with the theoretical value of the licenses set by the Comptroller and Auditor General in arguing for a transparent auction. And it doesn't let replacement telecom minister Kapil Sibal off the hook for defending "the indefensible: sale of 2008 spectrum at a non-market determined price of 2001," Jagannathan points out.
Here's his eight "take aways" from the damp squib.
1. More corruption?
"First, there was probably a degree of collusion or prior agreement among the big players that they will not bid seriously. This needs to be investigated."
2. More corruption?
"Second, it is highly unlikely that the government would not have known how the bids would go. Bharti Chairman Sunil Mittal gave the game away when he said the auction would be over in a day."
3. Bad timing.
"Third, the failure is more the result of wrong timing and bad investment climate – both of which are the result of the UPA's inability to manage its policies right over the last three years. If the 3G auction timing came when business optimism was rising, this time it was the exact opposite. Moreover, the auction may have fared better without a high floor price."
4. The time for cheap licenses is finished.
"Fourth, there is no case for pricing spectrum low – especially when India has achieved high teledensity."
5. Back to first-come, first-served?
"Fifth, the government should not now make the mistake of lowering the reserve price. Instead it should allot spectrum at the reserve price to whoever needs it. It can be first-come-first-served again – and this time it could work better since players would have no opportunity to collude."
6. Government-fixed prices, instead of cartel-fixed prices
"Sixth, since the one-time spectrum fee and other prices are decided by the auction, the government will have to fix new prices for these. It should now fix them on the basis of the reserve prices and not buckle under the pressure from industry."
7. Foreign investment can beat corruption?
"Seventh, some policy changes will change the picture. If, for example, 100 percent stake is allowed to foreign players, it would be impossible for the spectrum price to remain unbid for long. This is a key correction needed in policy since it is clear that many Indian players do not have the money to grow the market and are in the game only to collect rents."
8. More corruption?
"Eighth, the fact that big players like RCom and Reliance did not bid means the failure was preordained. Wonder why the government never asked why. The first clue came when the CDMA auction had no bidders."


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This group of musicians seeks to serve Ascension by arranging fresh and creative musical worship for the people who attend the 9 am service. The style ranges from contemporary to folk-style shape note singing to taize chanting. We have volunteers who play guitar, cello, violin, drums, piano, bass and more! Though often contemporary in vibe, we spend a lot of time reworking our favorite ancient hymns and scripture texts, sometimes with new tunes and sometimes embracing the most traditional tunes. We are happy to incorporate classically trained musicians as well as those who play by ear. We work together to produce songs that draw the congregation from a place of observing to a place of belonging and participating.
If you play an instrument, like to sing, or want to learn more about the music we do at 9am, please contact Chris Massa! We always love to incorporate new musicians into our ministry.


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Background: Since 2001, approximately 2 million troops have been deployed to Operation Enduring Freedom/Operation Iraqi Freedom (OEF/OIF). Service members are returning with a variety of physical and psychiatric conditions. Approximately 15-19% of returnees have been diagnosed with traumatic brain injury (TBI), which is associated with affective, cognitive, somatosensory, and vestibular symptoms. A major concern following mild TBI is the effect of brain injury on psychiatric conditions, such as posttraumatic stress disorder (PTSD), anxiety, and depression. Given the frequent comorbidity of these disorders, clinicians struggle in distinguishing between and treating the physical and psychological manifestations. Because of VA's organizational structure for polytrauma (PT)/TBI care, providers could potentially treat physical (e.g., TBI) and mental (e.g., PTSD) health symptoms in isolation from one another. The pervasiveness of physical and psychiatric sequelae in this cohort necessitates organizational structures that facilitate communication and coordination across disciplines to better address patients'multiple needs. Better coordination has been associated with better clinical outcomes in various settings, including in VA. Objectives: Focusing on the VA Polytrauma System of Care Polytrauma Network Sites (PNSs) and Polytrauma Support Clinic Teams (PSCTs) that provide care for OEF/OIF Veterans, we will examine simultaneously organizational factors, specifically structural integration (i.e. organizational and physical structures) and coordination processes (i.e. programming, feedback, relational coordination) and patient- related factors that are associated with PT/TBI clinic differences in healthcare utilization and patient outcomes, like community reintegration and satisfaction with health care. A secondary aim is to focus on gender differences in patient outcomes. To address our primary goal, we will address four research questions (RQs): RQ1) How do PNSs and PSCTs differ among and between themselves on three measures of coordination, when indices of structural Integration are considered? RQ2) Do patient characteristics, structural integration and coordination processes variables predict patient utilization? Is utilization associated with TBI status and referrals from the TBI evaluation clinic stop? RQ3) To what degree do patient characteristics, utilization, and structural integration and coordination processes predict differences in the patient outcomes of community reintegration and satisfaction with health care? RQ4) Based on the outcome of RQ3, what are the barriers to and facilitators of achieving the combinations of organizational characteristics and patient characteristics associated with better patient outcomes? Methods: This is a mixed methods study consisting of three phases. Phase 1 is a prospective quantitative study that will examine the PT/TBI clinic structural integration and coordination processes among employees of the PNSs and PSCTs via web survey. Phase 2 will involve a retrospective administrative database review of a large OEF/OIF cohort that was evaluated for TBI. Using VHA administrative databases, we will identify a Veteran sample (based on 8 PNSs and 16 PSCTs with adequate response rates from Phase 1) to examine their healthcare utilization 90 days post-TBI evaluation. This phase will also include a prospective survey of these OEF/OIF Veterans on (a) demographics, (b) health status, (c) community reintegration, and (d) satisfaction with VA healthcare services. In Phase 3, we will (a) select the 8 PNSs from Phase 2 and, based on the Phase 2 community reintegration survey measures, (b) identify the top 4 and bottom 4 performing PSCTs so that we may conduct qualitative interviews with PT/TBI patient healthcare providers to obtain a richer understanding of the organizational factors and coordination processes at these sites.
This proposal responds to OR&D's Deployment Health Research solicitation: OEF/OIF Veteran research issues on patient and system-level outcomes, and to HSR&D's (1) Priority A, addressing such topics as (a) impact of different organizations and structures/processes of care on access for specific populations (e.g., regionalization of care), (b) care access for Veterans with special needs (e.g., TBI, PTSD), and (c) vulner- able populations (e.g., women, those with multiple comorbidities);and (2) Priority F, for women Veterans' healthcare delivery, examining (a) care models, (b) patient characteristics, and (c) Veteran satisfaction. This research will identify structural integration and coordination processes within the Polytrauma System of Care that represent best practices to foster effective rehabilitation programs for OEF/OIF Veterans (secondary focus on women) with multiple, chronic conditions. These findings will inform future implementation studies that policymakers and healthcare managers need to translate research findings into policy and practice.


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Farewell 1999 Trailer (2003)
09 January 2003 26 mins
Farewell 1999 is a documentary that could move you to tears. Acclaimed director Wu Tai Ren takes viewers on a private and emotional journey contemplating the consequences of life and death, as she searches in her life for traces left by her late mother, who passed away four years ago. Wu captures her mournful self on film with a precise control over the narrative, trying to hold on to the memory of a loved one, while bidding farewell to the haunting sadness of death. Personal yet universal, the feelings conveyed in Farewell 1999 tug at the heartstrings as well as showcase Wu's talent that won her multiple international awards.
Watch the official Farewell 1999 2003 trailer in HD below or find more Farewell 1999 videos on Vidimovie.


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Girl's Swim Tights UPF 50+
No need for her to change clothes in the care or worry about keeping sunscreen on her knees with these tights that wear like pants but go into the water like a swimsuit and still protect from the sun with sunblocking fabric.


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Microbial Analysis of Soil Essay
Microbial Analysis of Soil
Abstract: soil samples were collected fortnightly from area near Dahisar River, A river in suburb of Mumbai. laboratory analysis started from July 2010 to September 2010. Total bacterial and fungal count were estimated by standard spread plate isolation. Isolated bacteria were subject to colony characterization and were estimated by their morphological and biochemical characters. As being a monsoon the occurrence of variation of different species were high. The microorganisms isolated from the soil were of staphylococcus strain and were gram positive, aerobic, coccus shaped bacteria. The fungal species were also identified, of which Aspergillus and Penicillium were dominant, followed by mucur, as sub dominant .This project aims to find out the water and soil quality of River and as it is flowing through an industrial area, to find out if it is getting affected by the Industrial pollutants.
Soil is the region on the earth's crust where geology and biology meet, the land surface that provides a home to plant animal and microbial life (Pelczar et al., 1993). Soil teems with microscopic life (bacteria, fungi, algae, protozoa and viruses) as well as macroscopic life such as earthworms, nematodes, mites, and insects, and also the root systems of plants. The numbers and kinds of micro- organisms present in soil depend on many environmental factors: amount and type of nutrients available, available moisture, degree of aeration, pH, temperature etc (Prescott et al., 1999). Soil bacteria and fungi play pivotal roles in various biochemical cycles and are responsible for the recycling of organic compounds (Wall and Virginia, 1999).
Soil microorganisms also influence above- ground ecosystems by contributing to plant nutrition, plant health, soil structure and soil fertility (O'Donnell et al., 2001). Soil is generally a favorable habitat for the proliferation of microorganisms, with micro colonies, developing around soil particles.
Numbers of micro organism .
In soil habitats normally are much higher than those in fresh water or marine habitats (Atals and Bartha, 1998). Bacteria make up the most abundant group of micro- organisms in the soil (3.0 x 106 – 5.0 x 108) per gram of soil, followed by the actinomycetes (1.0 x 106 – 2.0 x 107), fungi (5.0 x 103 – 9.0 x 106), yeast (I.0 x 103 – 1.0 x 106), algae and protozoa (1.0 x 103- 5.0 x 105) and nematodes (50 – 200) counts per gram of soil are wide differences in the relative proportions of individual bacteria genera found in particular soils (Atals and Bartha, 1998). Soil fungi may occur as free-living organisms or in mycorrhizal association with plant roots.
Fungi are found primarily in the top 10 cm of the soil and are rarely found below 30 cm. They are most abundant in well-aerated and acidic soils (Domsch et al., 1980). Most fungi in soil are opportunistic (zymogenous). They grow and carry out active metabolism when conditions are favorable which implies adequate moisture, adequate aeration and relatively high concentrations of utilizable substrates (Postage, 1994; Miyanoto et al., 2002). In this research we isolate culturable heterotrophic bacteria and fungi from different top soil samples MATERIALS AND METHODS
Laboratory analysis
Preparation of materials
The materials needed for this experiment include; glass wares (conical flasks, bijou bottles, pipettes, petri-dishes) and they were washed with detergents. These glass wares were rinsed thoroughly with clean distilled portable water and left to air dry before sterilizing them in the autoclave at 15◦C for 1 hour. Also, the laboratory cabinets on which the work would be carried out was swabbed with cotton wool soaked in methylated spirit to sterilize it before any microbiological analysis was carried out to avoid the growth and isolation of other organisms not present in the samples. After sterilization, the plates were allowed to cool to about 45 degrees before they were used.
Microbiological evaluation
Ten (10) grams of the soil sample for microbiological evaluation was weighed into 9ml of sterile water. Preparation of serial dilution goes thus: 1ml of the original stocks solution was poured into 9ml sterile distilled water and mixed thoroughly to give 10-2 of the original sample and this was done for each sample and the bottles labeled according to date of collection Isolation and Enumeration of Micro-organisms.
1gram of the samples was homogenized in 9mls of distilled water to obtain a ratio of 1:9 and the second diluted of each sample was plated using the pour plate technique. Sterile molten nutrient agar (NA), potato dextrose agar (PDA), macconky's agar,(MA) manitol salt agar (MSA) and deoxycholate astrate agar (DCA) were used{the potato dextrose agar (PDA) was acidified). These agars were then added and left to solidify undisturbed. These plates were incubated 37oC for 24hours (incubation was aerobic) and the procedure was repeated using 10-2 finally the number of colonies per plates were counted and recorded. The acidified PDA was incubated at 25C for 3-7 days for microbial growth.
Total Bacterial counts (Cfu/g)
The total bacteria count for each sample was determined with the pour plate techniques using nutrient agar. The plates were incubated between 24hours at 370C and all colonies appearing on the end of the incubation period were counted using digital unlimited colony counter and the counts were expressed in colony forming unit per gram {CFU/g} of the sample. Colonies of bacteria developing on the plates were observed, isolated and reisolated on a fresh media until pure culture was obtained.
Preparation of Pure Culture
It is necessary to isolate organisms in pure culture before studying and identifying them because a pure culture originates from one cell. Characteristics colonies from the original culture on the plates were picked with a sterile wire loop (using surface streaking method) and this loop was used to make streak of the colony on the surface of newly prepared sterile agar plates of NA,MA & MSA. These streak will space out the inoculants and discrete colony of a particular specie of organism and then incubated at 35-37oC for 24hours to enhance microbial growth. Distinct colonies were re-inoculated on another fresh agar plates in order to obtain a pure culture. The isolates were picked with sterile loop and streaked into prepared agar slants, labeled and incubated for growth after which they were kept in the refrigerator for future use and identification.
Identification of Isolates
These isolated bacteria were identified using both morphological culture characteristics (i.e. the color, shape, elevation, capacity, consistency, edge) and biochemical test (i.e. citrate, oxidase, indole, sugar fermentation, test etc.)and the bacteria were identified based on the results obtained from the above mentioned biochemical characterization results and the procedures include.
Grams Staining Techniques
A drop of distilled water was placed on a clean glass slide. The inoculating wire loop was sterilized by flaming until it was red hot (this is to prevent the invasion of unwanted micro- organisms that might be inhabiting the wire loop) in the blue flame of a Bunsen burner. The loop was allowed to cool and the small portion of each colony of microorganisms to be gram stained was picked and smeared in the drop of water (distilled) on the glass slide and then spread into a thin smear along the slide. The smear was air dried and passed through the blue flame. The smear was stained with 1%crystal violet and left for 1minutes (60secs) and then washed with running distilled water it was then stained again with Lugols iodine for another 60secs and also washed with running distilled water. The slide was decolorized rapidly with 75% alcohol in order to present the organism from having the color of the primary reagent and it was washed immediately with distilled water.
The slide finally was flooded with a counter stain safranine (a secondary stain) for 60secons and also washed off with distilled water and allow to air dry. The slide was covered with a cover slide and observed under the microscope using oil immersion x 100 objective lens with immersion oil. The gram reaction of the isolated arrangement and the shape of the cell were observed and recorded. Gram positive (+ve) bacterial were characterized by a purple color (i.e. the primary stain) while the gram negative (-ve) bacteria were characterized by red color (i.e. the secondary stain) .This procedure is actually used to ascertain the component of each organisms cell wall.
Motility was determined by hanging drop techniques. Using loop, a little part of the colony of the organisms were grown in peptone water for 18hours and then placed in the grease free slide and covered with a Vaseline bound cover slip and then observed under x100 objective lens. A motile organism is then seen moving in the drop of liquid.
Identification Of Mold Isolates
Mold isolated was identified using cultural and morphological characteristics and according to (Fawole and Oso, 2001), microscopic observation was carried out using lacto phenol blue stain.
Procedure for Mold Staining
A drop of lacto phenol blue stain was dropped on a clean grease free sterilized glass slide and after this a sterile inoculating wire loop was used to pick the mycelium unto the glass slide from the mold culture .The mycelium was spread evenly on the slide. Teasing was carried out to separate the mycelium in order to get a homogenous mixture and the mixture was then covered with cover slips gently and then allowed to stay for some seconds before observing under x40 under the microscope. The microscope examination of actively growing mold was on the basis of structures bearing spores, presence or absence of septate.
Catalase Test
Catalase test demonstrates the presence of catalase enzyme by aerobic microorganisms. Catalase is an enzyme that catalysis the release of oxygen from hydrogen peroxide (H2O2). To test for catalase, a drop of 3% hydrogen peroxide solution was added to a slide and the organism to be tested for catalase production is brought in contact with the hydrogen peroxide. The production of gas bubbles however indicates a positive reaction and this shows that catalase enzyme is produced.(Fawole&Oso, 2001)
Oxidase test
This was carried out by placing a clean filter paper on the working bench or petri dishes and 2-3 drops of freshly prepared oxidase reagent was added to the isolate using a sterile inoculating wire loop. After this, a few quantity of oxidase reagent was added and a purple coloration was observed within 10-15minutes which indicated that the organisms is oxidase positive and according to Olutiola et al, 1991, a positive reaction is dependent on the presence of cytochrome. This test is also useful for the separation of Neisseria in mixed culture and in differentiating Pseudomonas from enteric bacteria.
Indole test
Olutiola et al, 1991, describes the test as one which is important in the differentiation of colonies and it depends on the production of indole from tryptophan by the organism. An inoculating loop was used to inoculate the organism into a test tube containing decarboxylase medium becomes violet. An uninoculated test tube serves as a control (i.e. remained yellow)
Sugar fermentation test
The ability of the isolates to utilize certain sugar as energy source was tested. If the organism does ferment a particular sugar, acid will be produced and gas may be produced or not. Acid production is indicated by color change of the medium from red to yellow and acid presence could also be detectable with a ph. indicator in the medium while the production of gas is indicated by a void produced in a Durham tube. The fermentation medium was prepared by 0.1g of sodium chloride and 0.1g of fermentable sugar (glucose) in 10ml of distilled water.
An amount of 9ml of the medium was pipette into a test tube containing Durham's tubes in replicates. 5ml of phenol red indicator was immediately discharged into the test tubes. The test tubes containing medium were sterilized in an autoclave at 121 o for 15minutes.After sterilization, each isolate were incubated in glucose Medium. An uninoculated test tube was also incubated for glucose to serve as a control. The test was also carried out using maltose, lactose, galactose, manitol, sucrose, fructose and mannose.(Olutiolaet al., 1991)
The abundance of bacteria and fungi in this study were typical of environment with high species richness and functional diversity. Despite the fact that it is possible that a number of bacteria and fungi may be missed in this study, the isolates could be readily assigned dominant (e.g. Bacillus sp, Aspergillus sp) or transient/succession roles in the isolation of organisms form different seasons, which form the basis of this study. In additions to the implications of the determination of the number of microorganisms during soil sampling, one should consider the qualitative aspect of the preservation of important species and groups of microorganisms and of the changes in these biochemical characteristics resulting from the variations in these counts.
Although the results of this study would not be considered to be exhaustive, as it was done within the limits of facilities available in the laboratory, an insight into the population dynamics and distribution of culturable aerobic bacteria and fungi diversity has been elucidated. This is without prejudice to the possible influence which a substantial proportion of bacteria and fungi that are not culturable in vitro could have on the overall picture of event. It would require more modern technology (nuclei acid probes) to obtain such detailed overview of microbial diversity. This should be a subject of extension of this investigation in future.
Through this project, if emphasis is made on public health, the observation and findings show striking predominance of Salmonella typhi. And E.coli. E.coli being an enterobacter cause dysentery and S.typhi poses a great risk of typhoid. Health inspector and municipal authorities should look into this matter for further investigation and if possible improvement
Investigators are grateful to the Principal & Management of S.V.K.M's Mithibai College for constant encouragement & support. And head of department of zoology Prof. V.V. Dalvie for providing me opportunities and Prof. Radhika D'souza, under whose guidance the project was successfully completed
1 .Atals RM, Bartha R (1998). Microbial Ecology: Fundamentals and Applications. 4th Edition. Benjamin Cummings Publishing Company Inc. Addison Wesley Longman Inc. pp. 300 – 350. 2. Miyanoto T, Igaraslic T, Takahashi K (2002). Lignin–degradation ability of litter decomposing basidomycetes from picea forest of Hokkaida Myco.sci. (41): 105 – 110. 3. Domsch KH, Gaws W, Anderson TH (1980). Compendium of soil fungi
4. O' Donnell AG, Seasman M, Macrae A, Waite I, Davies JT (2001). Plants and Fertilizers as drivers of change in microbial community structure and function in soil. Plant Soil (232): 135 – 145. 5. Pelczar MJ, Chan ECS,
krieg NR (1993). Microbiology: Concept and Application International edition McGraw-Hill, USA. Pp 281-324. 6. Wall DH, Virginia RA (1999). Controls on soil biodiversity insights from extreme environments. Appl. Soil Ecol. (13): 137–150. 7. Fawole and Oso, 2001
Subject: Bacteria,
University/College: University of Chicago
Type of paper: Thesis/Dissertation Chapter
Date: 29 October 2016
Let us write you a custom essay sample on Microbial Analysis of Soil
for only $16.38 $13.9/page


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Lottery numbers
Wed., July 27, 2005
Tuesday's Washington Daily Game: 2-3-5.
Tuesday's Washington Keno: 5-9-10-20-25-
Tuesday's Mega Millions: 1-10-18-29-55.
Mega Ball: 8
Today in history
1789: Congress established the Department of Foreign Affairs, the forerunner of the Department of State.
1866: Cyrus W. Field finally succeeded, after two failures, in laying the first underwater telegraph cable between North America and Europe.
1953: The Korean War armistice was signed at Panmunjom, ending three years of fighting.
1960: Vice President Richard M. Nixon was nominated for president at the Republican National Convention in Chicago.
1974: The House Judiciary Committee voted 27-11 to recommend President Nixon's impeachment on a charge that he had personally engaged in a "course of conduct" designed to obstruct justice in the Watergate case.
1976: Air Force veteran Ray Brennan became the first person to die of so-called Legionnaire's Disease following an American Legion convention in Philadelphia.
1980: On day 267 of the Iranian hostage crisis, the deposed Shah of Iran died at a military hospital outside Cairo, Egypt, at age 60.
1996: Terror struck the Atlanta Olympics as a pipe bomb exploded at Centennial Olympic Park, killing one person and injuring more than 100.


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Iggy Azalea Claims She Could be Underage in Alleged Sex Tape
Description:Iggy Azalea reportedly claims that a sex tape involving her and a scorned ex may exist, but it also may have been filmed without consent and while she was underage.
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A Phase I/II Trial of AMG 102 and Erlotinib in Previously Treated Patients With Advanced Non-Small Cell Lung Cancer
Patients must have baseline evaluations performed prior to the first dose of study drug
and must meet all inclusion and exclusion criteria. Results of all baseline evaluations,
which assure that all inclusion and exclusion criteria have been satisfied, must be
reviewed by a Physician Investigator prior to enrollment of that patient. In addition, the
patient must be thoroughly informed about all aspects of the study, including the study
visit schedule and required evaluations and all regulatory requirements for informed
consent. The written informed consent must be obtained from the patient prior to
enrollment. The following criteria apply to all patients enrolled onto the study unless
otherwise specified.
Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Treatment
Determination of a safe dose of AMG 102 when combined with erlotinib for the treatment of patients with advanced, previously-treated NSCLC (phase I part).
The recommended phase II dose is determined in part by the dose of AMG 102 that, in combination with erlotinib, is associated with a maximum 25% rate of dose limiting toxicity (DLT). Dose de-escalation will proceed according to a Narayana k-in-a-row design and the selected dose will be estimated by isotonic regression. For this trial k is set to 3.
Ongoing from the start of the study.
Ahmad Tarhini, MD
Principal Investigator
University of Pittsburgh
United States: Food and Drug Administration
August 2011
June 2014
|University of Pittsburgh Cancer Institute
|Pittsburgh, Pennsylvania 15213


text:
Hi Ya'll, we added a 2008 Impreza 2.5i to the roster this last weekend. In the fury of paperwork crossing the desk of the salesman, we signed up for the Subaru Added Security Maintenance Program.
Question is: Do we REALLY need it? Has anyone ever used it and what was you experience?
Todd in Seattle,
91 Loyale "Black in Black"
08 Impreza 2.5i "too new for a name"
Welcome to Ultimate Subaru Message Board, my lurker friend!
|Welcome to Ultimate Subaru Message Board, an unparalleled Subaru community full of the greatest Subaru gurus and modders on the planet! We offer technical information and discussion about all things Subaru, the best and most popular all wheel drive vehicles ever created.
We offer all this information for free to everyone, even lurkers like you! All we ask in return is that you sign up and give back some of what you get out - without our awesome registered users none of this would be possible! Plus, you get way more great stuff as a member! Lurk to lose, participate to WIN*!
* The joy of participation and being generally awesome constitutes winning
** Not an actual guarantee, but seriously, you probably won't regret it!
Serving the Subaru Community since May 18th, 1998!
Guest Message by DevFuse
Subaru Added Security Maintenance Program
Started by Todd in Seattle , Jan 08 2008 10:23 PM
1 reply to this topic
Posted 08 January 2008 - 10:23 PM
Posted 10 January 2008 - 12:32 PM
What exactly do you get with the program? Is there some documentation you received?
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Mirror of Kalandra*
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The Introduction of Lacerate Skill Path of Exile
This is a very interesting skill of close combat, range and attack. You can get a lot of range bonus, but seeing a lot more advanced double hands damage.
The Skill of Berserker Ice Bow Path of Exile
The main skill is Ice Shot and Barrage. This is a mainstream career. There are threw sublimation talent which provides a large number of injuries and attack speed, clear mobs and BOSS efficiency greatly improved!
BD Developed by the New Flask Path of Exile 2.6
The Bismuth Flask comes with 35% total Elemental Resistances, and it can be up to 53% Elemental Resistances in the BUFF. Tornado basically shoot with no dead angle in response to the group.
Advanced Skill on the Calculation of Penetration Path of Exile
Penetration is the resistance to elemental damage, used to reduce the damage to your own elements. Elements penetration is a way to reduce target element resistance.
How to Play BD Ice Whirlpool Totem of Path of Exile
To play bd ice whirlpool totem, you have to learn about the vortex, burning flame ring. Have a look at the details in the text.
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text:
Ferrari's most secret supercar has been doing the rounds of potential customers. A leaked letter has confirmed the convertible version of the 599 GTB was viewed at the recent Concours d'Elegance at Pebble Beach in the US.
The exclusive Drive Life illustration is the best look you'll get at the car unless you buy one.
Attendees at the preview had to pass through multiple security points, sign a non-disclosure agreement and hand in any mobile phone or camera they were carrying.
Far from the more practical folding metal hard-top of the Ferrari California, the car on display featured a part-time fabric roof with a removable glass rear window, according to unconfirmed reports.
One attendee has claimed it is only for occasional use, similar in style to Porsche's Boxster Spyder temporary canvas roof.


text:
3-PACK Crazy Dog Train-Me! Training Treats Chicken Flavor (3 lb)
Item: C98717PK3
Availability: In Stock
Customers Also Bought
Customer Reviews
DISCLAIMER: The results described on the testimonials on this site, are the individual experiences and personal opinions of those who have purchased and used the products. These testimonials are not intended to make any claims that the products can be used to diagnose, treat, mitigate, cure or prevent any disease or medical condition.


text:
PAIR 1931 Georgia License Plate Tag
Sold for:
Start Free Trial
or Sign In to see what it's worth.
- Sold Date:
- Source: eBay
PAIR 1931 GA License Plates in good original condition. These were the depression era theft proof plates and unfortunately, the locking tabs have been broken off upon removal Due to popular demand, FELIX1 and UPTOWN BUTTE will continue to run auctions on ebay as well as sell license plates from their site and warehouse. However, some of the 'rules' have changed with our ebay sales, so please read below. Payment by PayPal or other accepted form of payment allowed by ebay. We now take our pictures with a digital camera.POSTAGE AND SHIPPING : Shipping in the US is $6 for the first plate, $2 for the 2nd, and $1 for each plate thereafter. (Pairs count as 2 plates.) Canada shipping is $8 for first, and $2 for each additional plate . We do reserve the right to charge more for heavier plates such as porcelains and heavy long plates mostly from the New England States. . We are sorry but we no longer ship outside of the US and Canada because of customs delays and disputes about postage costs.. We reserve the method of shipment of plates (priority, flat rate, or first class), although all go via US postal system . WE ARE HAPPY TO COMBINE auctions to save you postage so long as they are paid for in one payment, and will reduce postage costs for auctions over 20 plates if we can send more economical. Please include item numbers and pay ... read more


text:
Unplugged: Middle School Day Camp (entering grades 6-8)
Tweens (11-13) will enjoy the challenges of this program, which tackles STEAM – 19th-Century style. They'll build pinhole cameras and develop the photos in a darkroom; build a penny battery, compete in a Morse Code challenge; attempt the Pioneer obstacle course, learn to read a compass, and much more.
The camp takes place August 7-11, 10 a.m. – 3 p.m., and costs $160. Online registration begins in late March.


text:
Gamespy Lists Battlefront 3 in their Server Lobby…
I thought that this was quite interesting and a big thanks to my fellow commenter for bring this to our attention. Apparently Gamespy.com has listed Battlefront 3 in their game lobby.
I have a program called GsList that allows me to see the servers for every game run by GameSpy so I can basically see the lobbies for them. Like I used it to see what servers were online and how many players were in them for SWBF2.
I decided to check it out today and to my surprise I find this:
When I click on them no servers show up, but those lobbies were not there a month ago.
This looks increadibly promising.
You can get GsList here if you want to see for yourself.
Use the GSListWeb executable.
Oh BTW the reason there is no Xbox stuff on there is because Microsoft doesn't allow GameSpy to host lobbies for games over Live. They do it themselves.
This is definitely interesting… i'll be interested to hear all of your opinions. I know that the software program isn't put out by GameSpy, so who know's it could most certainly be fake.
It appears I forgot to add the proper credit, which I apologize for.
Source: SG-17


text:
Men's 10" Composite Toe Composite Plate Oil Transitional Boots
Description / What it's For
These mid-calf height, full grain leather boots are waterproof and treated for diesel and oil resistance. A transitional boot, they are designed for drivers, equipment operators, couriers and other trades requiring protection from wet or slippery environments. When the mercury drops they feature a removable T-MAX insulation liner that regulates the temperature for maximum comfort. Green Diamond technology on the rubber outsole provides sturdy traction and control, while the TPU toe cap extends the life of the boot.
Style: 5ANFDKW18901GD
Features / How it works
For long-term durability
Waterproof/Breathable protection
Our lightest, warmest insulation
Antimicrobial, stays fresh
For better grip and traction
CSA Grade 1 composite toe and plate, ESR - Electric shock resistant
Drivers, equipment operators, couriers, trades where a transitional boot is required rather than a felt pack
Men's 10" Composite Toe Composite Plate Oil Transitional Boots is rated out of 5 by 29.
Rated 2 out of 5 by EMDFan from Expected better Pros: Warm, Comfortable, great grip on ice. Cons:Poor construction, not very durable. Got about 6 months of use out of these(used only during winter), before the sole completely fell apart(split and cracked in several places. Expected more out of a $250 pair of boots. Might be good for use in industries where oil and diesel aren't present, but for a railroad diesel mechanic...not so much. Disappointing, because other than the durability being subpar, they are warm and comfortable boots.
Date published: 2017-01-28
Rated 5 out of 5 by Mct1 from Light weight, warm, long lasting! I work outside as a blaster and I gotta say that there's no better boot out there than these. Really light compared to most boots and rather small for how warm they are. I'm not gonna lie, if your just standing in the cold and not moving you do get a a bit cold, but what boot doesn't. I've had 2 of the previous pairs and they both lasted 3 winters. Probably could of got more out of them if the explosives didn't eat away at the boots. Awesome boot! Recommend to all!
Date published: 2017-01-07
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Date published: 2017-01-04
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Date published: 2016-12-25
Rated 5 out of 5 by Don D from Worth every penny !! The best boots I ever bought , first boots they claime to be water proof and they actually are , never got my feet wet in those , and they are warm , half a day through -20 bellow and you can feel your feet are worm , there lite and comfy were them all day no problem , they run a bit small , I weir a 10"and needed an 11" with this one . The laces don't last , you might want to buy a spaire set at your purchase.
Date published: 2016-12-20
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Date published: 2016-12-09
Rated 1 out of 5 by Railroad from Poor fitting Ordered on line size 9 very tight fitting returned to store tryed size 10 ok but very difficult to lace up would need to wear bell bottoms to fit pants over
Date published: 2016-12-05
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Date published: 2016-12-02


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GR7 At The Circuit Of The Americas
Don't just watch it, Drive it
Over the past six years of goldRush Rally, we've continually stepped up our track and driving events. This year we've apexed all of our previous track days with the once in a lifetime rental of the famed Circuit of The Americas (COTA) in Austin, Texas.
This world-class destination for premium sports and entertainment, is the first racing facility in the United States purposely built for Formula 1™ racing. COTA is home to the FORMULA 1 UNITED STATES GRAND PRIX, which was named the 2012 "Sports Event of the Year" by SportsBusiness Journal/Daily. The inaugural F1 USGP recorded a three-day attendance of more than 265,000 fans with 117,429 attending the race on Nov. 17, making the COTA's first Grand Prix the second-most attended Formula 1 race of the season. The 2013 F1 USGP attracted 250,324 spectators over three days' time, securing COTA's status as the United States' new home for F1 racing.
In June 2014, COTA hosted the prestigious spring/summer edition of the ESPN X Games, bringing thousands of extreme sports athletes and spectators from around the world to Central Texas for four days of international competition that was broadcasted globally. COTA bested competitive bids from top cities Chicago, Detroit and Charlotte, N.C., to win the games for a four-year period.
Since its grand opening in the fall of 2012, COTA has hosted more than 1 million visitors at events such as the world's premier motorcycle racing series, MotoGP™; V8 Supercars™, the most popular motorsport in Australia; and the popular U.S. series GRAND-AM Road Racing™, now known as United SportsCar Racing after merging with the American Le Mans Series. The Le Mans series was one of two endurance racing series, including the FIA World Endurance Championships, hosted at COTA in 2013, along with the U.S. Vintage National Championships and the first leg of the Tour of Austin, a popular bicycle race. COTA holds the unique distinction of being the only U.S. racetrack to host three World Championship auto racing series (Formula 1, MotoGP and World Endurance Championships) within one year's time.
The 1,500-acre complex features a variety of permanent structures designed for business, education, entertainment and race use, with only 350 acres fully developed. The 3.4-mile undulating racetrack was designed by the world-famous architectural and engineering firm, Hermann Tilke GmbH & Co., and features dramatic, natural elevation changes, including a 133-foot climb up to its signature Turn 1. The 20-turn circuit incorporates some of the best-loved and most challenging turns from other iconic Formula 1 tracks around the world.
COTA's paddock area is home to a 270,000-square foot Pit/Paddock Building with 34 garage bays and two upper levels of hospitality space. Additionally, the 44,000-square foot Event Center hosts business and social functions and serves as the venue's Media Center during major sports and entertainment events. Also among the facility's offerings is a massive grandstand with corporate suites, a chic, multipurpose lounge space and permanent seating for more than 9,000 guests. A state-of-the-art medical facility provides emergency medical care for competitors and spectators alike. The facility has on-site parking capabilities for approximately 25,000 vehicles, permanent depots for shuttle buses and taxis and an on-site heli-stop.
For more information and downloadable video, audio and photos, visit: www.CircuitofTheAmericas.com


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1 8-ounce package cream cheese
1 cup sour cream
1 jar marshmallow crème (7 to 8 ounces)
1 14-ounce can sweetened condensed milk
Assorted fresh fruit slices for dipping
Process in blender until smooth. Refrigerate at least one hour. Serve with chilled, sliced fruit with colored toothpicks for added fun.