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- 82 years old
- Date of birth: Jan 3, 1929
- Date of passing: Aug 2, 2011
|Let the memory of Stella be with us forever
"Forever in my heart ...love always ..."
Today is 2 years since you left to go home with grandpa. I wish you could see me today.all grown up, I am engaged to a beautiful woman. I miss you dearly and I love you with all my heart grandma and i hope you are happy and looking over us. I cant wait to see you in the future grandma. I love you and always will love you."
"I lived right across the street from you all my life! You were a dear and special friend to my Mother for all the years we lived there! You will be truly missed by all that knew and loved you, me included! God rest your soul dear lady. Always, Cathy Burns"
"Stella, thank you for being so kind and loving to me and accepting me as a part of your family over the past five years."
"Aunt Stella, I have so many wonderful memories with you, especially when I lived with you for a short period. You were the one whp taught me how to crochet when i was 14 :-) I'll miss you everyday until we see eachother again.xoxox"
"Grandma, I will always remember and love you and it brings tears to my eyes that I didnt get to see you to say goodbye, or as you say I'll see you later. You were the best part of my life growing up and I cherish you so much I will remember everything did you dd for me. I loveyou"
"I remember nothing but sweet things about Aunt Stella,
very, very loving. She will always have a special place in my heart."
"Dear Mom,
you made the last 17 years so perfect for me and my family. Dallas jr had his grandmother, I had my mother, whom I could share every day with and My husband Dallas had your love. thank you I love you"
"Dear Stella,
Your loving spirit will be with us all in our thoughts and memories of you. I pray you are dancing with the angels free from pain and earthly imperfections. God bless you eternally. - Laura Ramirez"
"You were a very loving and caring aunt...love you...."
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text: Before the Froyo update, whenever I wanted to stop playing mixzing or pandora, I used Advance Task Killer and it would stop playing. Now, after froyo, I have to go to the song, hit pause, hit the home button and that would stop the program. Has this happened to anyone else?

subset: commoncrawl


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Mobile Suit Gundam Federation Vs. Zeon
September 10, 2002 - This is a curious piece of work here, an apparently good Gundam game. If you don't normally follow the import scene, perhaps you don't notice the contradiction in terms there -- since time out of mind, Japan's most popular piece of giant robot animation has been nearly synonymous with videogame adaptations that run from the mediocre to the godawful, thanks to Bandai's traditional policy of letting the license pull in the customers. I've said a few nice things about Gundam games in the past, but most of them topped out at "If you're a fan, maybe..."
Mobile Suit Gundam DX Federation Vs. Zion (Import Review)
February 21, 2002 - I'll admit it; I'm a big otaku! When it comes to Anime, I can talk for hours on end. I play all of the great Anime games. Well, okay so most of them aren't so great. Probably 90% of those games are bad. But every once in a while an Anime game goes that extra mile and reaches the coveted 10% good Anime game category (it's rare, but it does happen.) I'm here to tell you that Gundam DX Federation Vs. Zion is just one of those diamonds in the rough. If you don't know what Gundam is, let me explain a little history on the Anime. The year is 0079, it is the far future and humans have colonized space. Amuro Ray a young teenager is forced into a war against Zion, a militant group tired of the Earth's Federation looming over them. One fateful day Amuro finds himself piloting the Federation's prototype mobile suit, Gundam and learns the cruel lessons of war. Learning the pilots in enemy mobile suits are normal people just like him, losing close friends and the pressure of if he fails his whole company will go down in flames are just some the horrors Amuro faces in Gundam.
If Nothing Else, Gundam Still Sells
December 12, 2001 - According to Bandai, its recently-released PlayStation 2 mecha fighting…
Naomi Gundam Coming To PS2
September 26, 2001 - Capcom and Banpresto have announced that the Naomi arcade title Mobile Suit Gundam -- Federation Vs. Zeon DX will be brought home to PlayStation 2 in Japan on December 6. Like Capcom Vs. SNK 2, the 3D mecha fighting game will be playable online via KDDI's Multi Match system, allowing as many as four mecha aces to do battle in a single theater of the One Year War.


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" I suspect as more developers have access to systems (Hue, Lockitron locks), we will begin to see a more complete integration across the board."
Hue, Yale, Schlage, Kwikset and the rest have reached out to Samsung and worked with them, hence being certified and native in their hub and show up as the if you want this sort of product this is what you should buy. They did not just wash their hands of it and say we have an API and maybe here is our IFTTT channel, have fun.
With supposedly so many Bolts being shipped in the US have yet to see much on the Internet from these owners. The last thing google shows is https://github.com/dkirker/smartthings-lockitron which was the original Lockitron and hasn't been updated in 2 Years.
So it appears your "more complete integration" will more likely actually be go learn groovy, hack something together yourself, hopefully share it with others on github, and pray everything works. Of course this will only be useful to those of us whom are savvy enough to be able to get into our backends and add smartapps and device handlers on our own, but not savvy enough to code from scratch.
The only way you will see true more complete integration is if just like Hue, they work with other parties to ensure their hub to hub communications, apps and devices work the way they should.
But all of this is a rather moot point with the status of bridge being what it is.
@Rattay780 @josh.stuckey moving your discussion about SmartThings here where it's more relevant.
To clarify, the other locks you mention support SmartThings because they are Zigbee or Z-Wave devices. They don't have their own stand alone apps - which, depending on your use case may or may not matter. So their is really no active integration there, SmartThings supports the spec, and as such can very quickly "certify" them.
With half a dozen significant platforms to integrate we've started with IFTTT as that lets us see where the demand is. Based on that data a direct Alexa integration is our next priority.
Hi Cameron, Good call on the thread move, got a little off the when will we see product over to and what if we see product there.
Ok, now to your points, and to Alexa, let's start with architecture. Most individuals will have architecture as follows when they deep dive into IoT, and when more consumers get involved:
Amazon Alexa / Google Home (Voice Layer)
Samsung SmartThings / Wink / Vera (Control Layer)
/ | \
Thing 3rd Party Hub Thing
3rd Party Things
Now that this is out of the way, more to companies and integration.
While you are correct those locks are Zigbee or Z-Wave that makes their lives simpler, and they have gone the slightly extra step of being in the app so they can be added manually, have connection instructions, and buy now links. Other more generic Z-Wave devices like the Econet EBV105 valve I recently picked up are slightly different Z-Wave in that they just show up as . But these are both irrelevant here as Lockitron is not Z-Wave and not like any of these products.
As Lockitron will supposedly have it's own bridge, this is where the integration would happen and not with the lock. When josh mentioned Hue, it is one example. SmartThings hub and the Hue bridge speak to each other over the LAN after button pairing, this is an approach, this was community developed. This same approach was that used by Chamerlain/Liftmaster where they have their own hub, but a clever coder made his own smart app and device handlers to allow tying into other things, https://github.com/brbeaird/SmartThings_MyQ this was also not developed by the manufacturer or Samsung. The Sonos integrations have actually been developed by the Samsung SmartThings folks with collaboration of Sonos, they allow limited usage outside of Sonos primary application, such as volume control play pause and track forward, offering limited control. This same approach was that used by Chamerlain/Liftmaster where they have their own hub, but a clever coder made his own smart app and device handlers to allow tying into other things, https://github.com/brbeaird/SmartThings_MyQ this is not by the manufacturer or Samsung.
My favorite, and the one that would be best equated with Lockitron would be like the folks of AlarmDecoder did for their AD2PI appliance. They created their raspberry pi based device, they created their API, and went the step further and also released https://github.com/nutechsoftware/alarmdecoder-smartthings on github which provides the device handler and smartapp for their product and their api they know intimately instead of making somebody off the street have to put it all together. It provides the ability to arm disarm, such as you would have lock / unlock. All of the fancy stuff would remain in the lockitron app and on your web page.
Perhaps the dkirker stuff still works, perhaps one of the folks that actually got a bridge will release something on github by the time the rest of us finally get our hubs. Or perhaps Lockitron can pick this up?
As for Alexa, or Google Home for that matter, those that make the home automation brains are already on that, wouldn't it be more low hanging fruit dealing with the brains which will then achieve the harder integration with the voice services with their larger budgets/teams of coders?
Of course not having the Bolt, and even less likely to see the bridge any time soon I am not in a position to deep dive into the API to see what is required here, nor be able to perform the coding and testing required to get things going and then unleash it for all of your customers to use.
@Rattay780 thanks for the thoughts; I'm a bit wary of going through a partial measure of building an integration and releasing on Github without fully going down the path of "Works with SmartThings" (i.e. it's still only for the most technically sophisticated folks and wouldn't add to the existing dkirker work). That said, we aren't ruling it out but can't commit the resources to it today.
I should note that there is a recent, patched branch from the dkirker repo that might help folks looking to play around with this: https://github.com/annbob/smartthings-lockitron/tree/patch-2.
Agreed to comments above re latency; the native Alexa integration will be significantly faster than doing it through IFTTT (but you will be limited to the native commands, unlike IFTTT).
Thank you very much for the link to the updated code on github as a starting point. I understand your thoughts in regards to full works with smart things path, hopefully something can be done with that existing code to tide over both communities until such time as that can be done. Would have thought that at least some of those who have purchased and received product by this point would have fallen into the technically sophisticated bracket.
Now if only somebody outside of your organization that would be willing to work on this for the good of the many could actually get their hands on both products required for development and testing.
Has anyone had any luck with using that code from github?
When I try to use it, I go to set up the smartapp, and it says Lockitron requiredWhen I tap there, it takes me to the lockitron api page and gives me a login screen.After I enter my UN/PW, it says An error has occurred The redirect URI is included is not valid.
Sadly have not been able to try, seeing as bridges are like leprechauns, some have claimed to have seen them however could just be rumors.


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Fears for missing Afghanistan aid workers
Concerns are growing over the safety of the eight Christian aid workers arrested in Kabul six weeks ago. The group, from the charity Shelter Now, were working on a project to provide emergency accommodation for refugees displaced by years of war and drought. Together with 16 Afghan staff, they were accused of trying to convert Moslems to Christianity - a charge which could carry the death sentence. See ICN 4 September 2001 Afghanistan aid workers trial begins today Initially the group were held in a detention centre in Kabul and appeared in court earlier this month to face charges. They were last seen by diplomats and family members, shortly before the terrorist attacks in America. Now all access to the city has been stopped and the whereabouts of the eight is unknown. The Red Cross is trying to locate the group, and has asked the Taliban for access. Mario Musa, Red Cross director in Peshawar, said the Geneva Convention gave them rights of access only to those detained as a result of conflict. He said: "In this case we have to rely on humanitarian considerations. It is extremely sad and desperately worrying for the families."


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The GoPro Hero Wrist Housing is perfect for sports where you require 2 hands! You can easily attach your GoPro to your wrist and wear it like a watch! Not only will it stay secure but you can also pivot the camera upright to shoot photos or video.
There are currently no questions and answers for this product.
There are currently no reviews for this product.


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News By Tag
Industry News
News By Place
Industry News
Lab Takes To The Skies By Establishing Drones Subsidiary For Video Content
Digital agency, Lab, has opened up a subsidiary to offer clients aerial photo and video content using drones
The subsidiary, Drone Media Technologies Ltd (DMT), will work with RPAS (Remotely Piloted Aircraft Systems), more commonly known as drones, initially to deliver media and film footage for a growing number of Lab's clients in the leisure and marine sectors, but it will also be investigating commercial applications for future growth and expansion.
A number of the Lab team have spent months in training to become qualified UAV pilots on their way to getting a permission for aerial work from the CAA (Civil Aviation Authority) – which only a few hundred people in the UK have. Lab is building a fleet of DJI S1000s carrying Canon 5D MKIIIs to deliver exceptionally high-quality video and photography work, and their next plan is to offer 4K video as an option.
In what is a hugely growing area for golf courses, estate agents, country houses, and businesses in the marine and leisure industries to market their products in a slightly different way, orders for aerial footage are already flying in to Lab and DMT, as more and more companies realise how filming with a drone could make their business really take off.
"As a digital agency, Lab loves to be at the forefront of technology and how this is changing the world, hence our focus on drones and the myriad of applications for which they can be used. They really are changing the world as we know it," said Jonny Tooze, founder and managing director, Lab.
"We already do a lot of video work for our clients, and taking this to the next level by offering aerial filming services (http://lab.co.uk/
To coincide with the launch of their drones business, Lab is putting on an event called Drones For Good, featuring high-profile speakers from across the world talking about how drones are changing the lives of people and businesses in remarkable ways, via their use in conservation, research, humanitarian efforts, precision agriculture, and the utilities and film industries, amongst others. Keeping with the aerial theme, the event will be held on 25 September 2014 at the RAF Museum London. Interested parties can register at http://dronesforgood.co.uk.
About Lab
Lab is one of the leading independent digital agencies in the UK, working with brands who want to give their customers personalised experiences online. With over ten years' experience in creative design, digital marketing, and web technologies such as Sitecore, Magento, and Drupal, Lab are experts in making sure the digital experience delivers results. Lab clients include Live Nation, the Department of Health, Whirlpool, Vintage Roots, Oakhouse Foods, Subway, SmartFocus, and Reed Learning. When it comes to digital, Lab believes everyone deserves A Better Experience™. Catch up with Lab at http://lab.co.uk
Huw Waters | Head of Marketing | Lab
0207 183 6668 (tel:0207%20183%
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For most professionals, building your personal brand and increasing your brand capital includes the use of social media. Online platforms such as LinkedIn, Facebook, Instagram, Twitter, etc. give you the opportunity to present who you are and what you are passionate about, and connect with others who share your interests and goals. Utilize these sites to advocate for the career ... [Continue Reading]
Be Careful Sharing Political Views on Your Social Networks
This election year, many professionals struggle with whether or not to post about their political views online. Could their employer be upset? Might their colleagues be offended? Would their clients admire their political position? Job seekers must also consider whether posting about politics (and politicians) can impact job prospects. There is a common belief that your ... [Continue Reading]
A Warning About SnapChat
I recently had a conversation with a colleague about the appeal of SnapChat. She told me that she loved the app for its silliness (adding funny filters and text to her images), quickness (she can post an image or video to her "story," and it immediately goes to all of her "friends") and temporary-ness. I asked her to explain her last point. Her response: "When you post ... [Continue Reading]
The New Trend in Social Media: Quality Over Quantity
Guest Blog by Kathi Kruse, speaker, social media, digital marketing consultant, and owner of Kruse Control In this world of cheap knockoffs, hidden agendas and mud-filled transparency, the reality is that not all social media connections are created equal. We're searching for authenticity in ourselves and our network and that leads to craving more meaningful ... [Continue Reading]
How to Connect with the Right People — And Keep Them on Your Side
Nearly everyone, from business professionals to job seekers, sales professionals to college graduates, recognizes that in today's competitive marketplace, relationships and networks are critical assets in a professional toolkit. I teach audiences how to use intentional networking strategies to gain introductions, leads, referrals, insights, and relationships with ... [Continue Reading]
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Native of Huntington, WV Lillie Bodie is a double major in Public Relations and Music Performance at Marshall University. Lillie is a waitress at the never-ending salad and breadsticks palace Olive Garden and is a part of the Black Scholars Society, Delta Zeta Sorority, University Chorus, The Heavy Hitters Band, and social media coordinator at WMUL. Lillie is a proud cat mom of two, Liza and Sebastian. In her free time Lillie enjoys singing, exploring, and impulsive shopping. Lillie aspires to challenge herself and reach her goals so after college she can be a traveling singer and document her journey.
Lillie Bodie, Reporter
|Jan 16, 2017
Students weigh textbook purchasing options (Story)


text:
Trundle Build Guide by
Not Updated For Current Season
This guide has not yet been updated for the current season. Please keep this in mind while reading. You can see the most recently updated guides on the browse guides page.
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Your votes and comments encourage our guide authors to continue
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Trundle Build
Ability Sequence
Not Updated For Current Season
The masteries shown here are not yet updated for the current season, the guide author needs to set up the new masteries. As such, they will be different than the masteries you see in-game.
I've been playing Trundle a lot recently, and have noticed that there are far too few guides for him. Trundle is a Jungle Champion, and should be played that way. Trundle can lane, but it just isn't optimal for him.
This guide is still a guide in progress, as I'm still learning Trundle myself, so if you have comments, questions, or suggestions please let me know.
Thanks, and enjoy the guide!
Pros and Cons
- Strong utility.
- High mobility (Great Movespeed, and CC reduction.)
- Good damage and survivability.
- Wonderful anti-carry.
- Fast jungle clear time.
- Moderately high learning curve.
- No gap closer.
- Melee range.
The rune setup is fairly standard for the most part. Flat armor and per level magic resistance are common to many builds. Splitting AD and armor pen is my personal preference, but I've seen AS, AD, ArP, and MS runes all work well on trundle.
Greater Mark of Strength x 3
- Flat attack damage to boost early game DPS, and help clear the jungle quickly.
Greater mark of desolation Greater Mark of Desolation x 6
- Flat Armor Pen aren't as useful for early jungle, but as an aggressive jungle-r, I've found that armor pen is much more useful, more so because the enemy jungle-r often picks Cloth Armor as their first item.
Greater Seal of Resilience x 9
- Fairly standard. Flat Armor runes help with early survivability, and prove to be much more effective then their per level counter-parts.
Greater Seal of Resilience x 9
- Fairly standard. Per level magic resistance scale much better then per level armor, and magic damage is not something you have to deal with while farming very often.
Greater Quintessence of DesolationGreater Quintessence of Desolation x 9
- Flat Armor Penetration, same reason as the marks.


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[hens-fawrth, -fohrth; hens-fawrth, -fohrth] /ˈhɛnsˈfɔrθ, -ˈfoʊrθ; ˈhɛnsˌfɔrθ, -ˌfoʊrθ/
from now on; from this point forward.
from this time forward; from now on
late 14c., from hence + forward (adv.).
late 14c., earlier henne forth (late Old English); see hence + forth.
Tagged: h
Read Also:
- Henchman
[hench-muh n] /ˈhɛntʃ mən/ noun, plural henchmen. 1. an unscrupulous and ruthless subordinate, especially a criminal: The leader of the gang went everywhere accompanied by his henchmen. 2. an unscrupulous supporter or adherent of a political figure or cause, especially one motivated by the hope of personal gain: Hitler and his henchmen. 3. a trusted […]
- Hencoop
[hen-koop, -koo p] /ˈhɛnˌkup, -ˌkʊp/ noun 1. a large cage or for housing poultry. /ˈhɛnˌkuːp/ noun 1. a cage for poultry n,n phr A woman's dormitory (1900+ College students)
- Hendeca-
1. a combining form meaning "eleven," used in the formation of compound words: hendecahedron. combining form 1. eleven: hendecagon, hendecahedron, hendecasyllable combining form Examples Greek hendeka 'eleven' word-forming element meaning "eleven," from Latinized form of Greek hendeka "eleven," from hen, neuter of eis "one" + deka "ten" (see ten).
- Hendecagon
[hen-dek-uh-gon, -guh n] /hɛnˈdɛk əˌgɒn, -gən/ noun 1. a polygon having 11 angles and 11 sides. /hɛnˈdɛkəɡən/ noun 1. a polygon having 11 sides


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Hi there and welcome! I'm so glad you're here.
So you are hoping to find out a little more about who I am and what I'm all about. Cool! I'm excited to get to know you too, but before we get started, you may want to subscribe to my blog. You don't want to miss a thing.
"I believe if you want to achieve anything, you have to start with a strong belief system. One that is far greater than yourself. I want to help you tap into your potential, define your path and start making a difference by finding the passion in your life."
Now a Little About Me…
My Career
As you explore my site, you will find a sprinkling of sales stuff. Since my career started in the sales distribution industry 33 years ago, I feel compelled to share some of it with you. I started as a phone and counter salesman in the electronic distribution industry where I later advanced to outside sales and then area sales manager.
Being responsible for all aspects of the sales process and customer service, I have come to fully understand one thing that is now embedded in my gut.
The one thing I have learned over the course of my career, is the fact that the potential to be great is in you just waiting to burst out. You simply have to tap into it to be the very best at what you do.
This is where I am now. I'm here to help you find your potential. I want you to get better at what you do. So let me invite you to stick with me on this journey. Together, we both can become better at what we do.
Enter the Digital World
My primary focus is to share with you my thoughts, ideas, observations and experiences on daily life, blogging, sales (and lots of other stuff) that I hope you will enjoy reading and will share with your friends. I hope that my attitude and inspiration will inspire you to be the best at what you do. Let me invite you to stick around long enough so that we can get to know each other and learn new things.
It's time to make a difference in your life and the lives of others by finding the passion in your life and having the courage to follow it.
And yes, I really do like a good cup of coffee.
Follow me here and on Twitter as we take the journey together.
This is how I want to be remembered.


text:
Great cover
Got this cover for my phone a couple of years ago. Working up a ladder. Dropped it, bounced on concrete and was still good. Brought a second one recently as needed another phone for another worker. Great cover, not to bulky.
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Quick Details
- Place of Origin: Guangdong, China (Mainland), Guangdong, China (Mainland)
- Brand Name: XHW, XHW
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|Home | About | Journals | Submit | Contact Us | Français
Tumorigenesis results from a series of genomic alterations that leads to progressive disorder of the normal mechanisms controlling cell growth, death and differentiation (Bishop, 1991). Our recent studies have demonstrated that the extent of DNA double-strand-break (DSB)-initiated genomic deletion in tumours is significantly increased in high-grade breast tumours (Shen et al, 2000). On the basis of these findings, we hypothesised that ATM, BRCA1 and TP53, the critical genes in the DSB checkpoint/repair pathway, might play an important role during breast tumorigenesis, and that defects in these genes could result in poor tumour differentiation. A causal link between breast cancer development and mutation of ATM, BRCA1 and TP53 has been found in familial breast cancer syndromes (Buchholz et al, 1999). However, the probability of finding ATM and BRCA1 mutations in sporadic breast cancer is low (Vorechovsky et al, 1996; Bay et al, 1998; Papa et al, 1998). In contrast, genomic deletions at the loci harbouring these three genes are relatively frequent (Lo et al, 1998; Rio et al, 1998; Shen et al, 2000). Genomic deletion represents one of the 'two hits' needed to inactivate tumour suppressor genes for cancer formation (Knudson, 1971). Recent studies have suggested that epigenetic mechanisms, manifested as abnormal protein expression, serve as the other hit, and are involved in abrogating the function of certain tumour suppressor genes (Jones and Baylin, 2002). In addition, as our understanding of cancer development extends beyond single-gene disorders to multigenetic disorders and aetiological pathway-wide abnormalities, it is tempting to speculate that the combined effect of several defective genes in a common antitumour pathway could lead to more poorly differentiated tumours. We therefore carried out the present study, using a highly homogenous study population and a multigenetic design, to examine the relationship between abnormalities (both genetic deletion and abnormal expression) in the most critical DSB checkpoint (ATM and TP53) and repair (BRCA1) genes and tumour grade.
The study subjects, 74 Chinese women with early-onset (35 years of age) breast cancer, were a subset of patients selected from our ongoing hospital-based breast cancer cohort (Yang et al, 1997; Shen et al, 2000). Breast cancer in Taiwanese (Chinese) women is characterised by a low incidence (Yang et al, 1997), early tumour onset (Lo et al, 1998) and novel genomic alterations (Lou et al, 1997; Shen et al, 2000). Owing to the low incidence of breast cancer, which suggests an overall lower effect of common risk factors, and its homogenous genetic background, the Taiwanese population has certain advantages for studying the effects of genetic variations. In order to obtain an aetiologically homogenous group of study subjects, which should help in elucidating the causes of tumour, we restricted our study to histologically confirmed, primary infiltrating ductal carcinomas (IDCs). Direct sequencing showed that none of the patients had germline mutation of the BRCA1 gene. Furthermore, no patient had a family history of breast cancer or of Li–Fraumeni syndrome (hereditary, and associated with mutant TP53) in their first-degree relatives, and no case of germline mutations in the ATM gene has ever been identified in breast cancer families in Taiwan, so these tumours were most likely sporadic. On grading using the Scarff–Bloom–Richardson system (Le Doussal et al, 1989), the number of IDCs of each grade was found to be similar. Institutional review board-approved informed consent was obtained from each patient prior to tissue collection. None of the patients had received neoadjuvant treatment or preoperative chemotherapy or radiotherapy, which could have caused up- or downregulation of gene expression. Paraffin-embedded tumour tissue and peripheral blood were obtained from each patient. To ensure that the tumour tissue samples assayed consisted of more than 90% tumour cells, laser capture microdissection using a PixCell laser capture microscope (Arcturus Engineering, Mountain View, CA, USA) was routinely performed on slides to collect the scattered tumour cells without normal cells. Somatic DNA was extracted from tumour tissue and genomic DNA from peripheral blood (normal control) using a conventional proteinase K-phenol/chloroform protocol (Shen et al, 2000). All specimens were stored at −80°C until analysed.
A PCR-based method was used to detect loss of heterozygosity (LOH) at loci within, or close to, ATM, BRCA1 or TP53. The allelic status of these genes was determined using six microsatellite markers, two for each gene: D11S1816 (11q22–11q23, close to ATM), D11S2179 (intron 34 of ATM), D17S1322 (intron 19 of BRCA1), D17S1323 (intron 12 of BRCA1), TP53 (intron 1 of TP53) and D17S786 (17p13, close to TP53). The sequences of the primers were: D11S1816, forward 5′-ATTGTGAAGCTAGGTGCTGGTG- 3′ and reverse 5′-AAAGAGATAAAACAGATTCTGGATG-3′; D11S2179, forward 5′-TAGGCAATACAGCAAGACCCTG-3′ and reverse 5′-GCACTGGAATACGATTCTAGCAC-3′; D17S1322, forward 5′-CTAGCCTGGGCAACAAACGA-3′ and reverse 5′-GCAGG- AAGCAGGAATGGAAC-3′; D17S1323, forward 5′-TAGGAGATGGATTATTGGTG-3′ and reverse 5′-AAGCAACTTTGCAATGAGTG-3′; TP53, forward 5′-CTTGTAGTCCTAGCTACTCAGCA-3′ and reverse 5′-CAAAACATCCCCTACCAAAC-3′; and D17S786, forward 5′-TACAGGGATAGGTAGCCGAG-3′ and reverse 5′-GGATTTGGGCTCTTTTGTAA-3′. The PCR amplification was carried out using 100ng of DNA from tumour tissue or peripheral blood, 0.4U of Taq polymerase, 0.2mM deoxynucleotides and 2.5mM MgCl2 in a total reaction volume of 10μl. The PCR conditions were 95°C for 12min to activate Taq polymerase, followed by 40 cycles of denaturation (95°C, 45s), annealing (55°C, 30s) and extension (72°C, 45s), the final elongation being performed at 72°C for 10min. PCRs were run in a GeneAmp PCR 9600 thermocycler (PE Biosystems, Foster City, CA, USA). PCR amplifications omitting template DNA were included in each experiment as a control for contaminating DNA, and a housekeeping gene (β-actin) was used as an endogenous control. PCR products were electrophoresed on a 377 ABI PRISM sequencer, and the fluorescent signals from the different-sized alleles recorded and analysed using GENOTYPER (version 2.1) and GENESCAN (version 3.1). For an informative marker (heterozygous for the two alleles in blood specimens), the locus was considered to display LOH when there was a four-fold or greater difference in the relative allele intensity ratio between the tumour DNA and normal DNA (Figure 1). Deletion of one allele of ATM, BRCA1 or TP53 was defined by either of the two markers showing LOH.
Tissue specimens were fixed in 4% neutral-buffered formaldehyde, embedded in paraffin and cut into 4μm thick sections, which were deparaffinised for 3 × 5min in xylene, rehydrated in graded alcohol and rinsed in Tris-buffered saline containing 0.1% Tween 20 (TBST). To improve antigen retrieval, dewaxed sections were immersed in 0.001M EDTA, pH 8.0, heated for 30min in a pressure cooker (TAC-10KS, Tatung, Taiwan), cooled to room temperature for 15min, then rinsed briefly in TBST. Endogenous peroxidase was blocked by incubation for 5min at room temperature in 3.5% hydrogen peroxide in TBST, followed by a TBST wash for 5min. Nonspecific binding of antibodies was blocked by incubation of the sections for 30min at room temperature with normal rabbit serum (1:5 in TBST). After washes with TBST, primary mouse monoclonal antibodies were added and the section incubated in a moist chamber for 1h at room temperature. Following several rinses, biotinylated secondary antibody was applied for 30min at room temperature, then the sections were rinsed and peroxidase-linked streptavidin (Dako, Copenhagen, Denmark) was added for 20min. The chromogen was developed with AEC (Dako, Copenhagen, Denmark) and the slides were lightly counterstained with haematoxylin to provide cellular detail. The primary monoclonal antibodies used were specific for ATM (undiluted, ATX 08, NeoMarks, CA, USA), BRCA1 (1:125 dilution, SG11, Zymed, CA, USA) or TP53 (1:50 dilution, DO-7, DAKO, Copenhagen, Denmark). In negative controls, the primary antibody was replaced with TBST. In addition, the staining of infiltrating lymphocytes, stromal cells or adjacent normal epithelial cells within the tumours served as a normal control. Three pathologists independently evaluated the results semiquantitatively, and any discrepancy was resolved by joint review. Using previously reported criteria (Yoshikawa et al, 1999; Angèle et al, 2000), we compared the tumours to the adjacent normal epithelium (in which most cells show positive staining for ATM and BRCA1) and classified those tumours with more than 25% of ATM- or BRCA1-negative tumour cells as showing reduced expression of these proteins, all others being classed as showing normal expression. Since mutant TP53 proteins generally have a longer half-life than wild-type TP53 protein, which leads to their nuclear accumulation (Finlay et al, 1988), tumours with more than 20% of nuclear TP53-positive tumour cells were classed as showing aberrant expression, all others being classed as showing normal expression. For simplicity, both the above cases ('reduced' expression of ATM and BRCA1 and 'aberrant' expression of TP53) are referred to as 'abnormal' expression in the main text.
The Mantel-extension χ2 test was used to examine the association between tumour grade and individual defective genes. Furthermore, to simultaneously examine all possible interactions between, and joint effects of, ATM, BRCA1 and TP53, multigenetic analysis based on logistic regression was performed. All statistical analyses were performed using SAS version 8.0 software (SAS Institute Inc., USA).
Using four intragenetic markers (D11S2179 for ATM, D17S1322 and D17S1323 for BRCA1 and TP53 for TP53) and two adjacent markers (D11S1816 for ATM and D17S786 for TP53), our results showed that, after excluding noninformative tumours (six out of 74 at ATM, nine out of 74 at BRCA1 and 10/74 at TP53), the overall frequencies of LOH were 36. 8% for ATM, 44.6% for BRCA1 and 53.1% for TP53 in these early-onset breast tumours. These frequencies are similar to those reported previously (Shen et al, 2000). As shown in Figure 2, even in grade I tumours, the LOH frequencies were 27.8% for ATM, 21.1% for BRCA1 and 30.0% for TP53. When an association between the frequency of LOH at individual genes and tumour grade (tumour differentiation) was examined using the Mantel-extension χ2 test for trends, BRCA1 and TP53 showed a trend to a significant increase in LOH frequency in more poorly differentiated tumours (P=0.003, P=0.009, respectively).
Immunohistochemical staining showed that the frequency of abnormal expression was 24.3% for ATM, 32.1% for BRCA1 and 28.4% for TP53, and that high-grade tumours tend to have an increased frequency of abnormal expression of these three proteins (Figure 3). When the frequency of abnormal expression of individual proteins was correlated with tumour grade using the Mantel-extension χ2 test for trends, there was a significant trend to an increase in abnormal BRCA1 expression with higher grade (P=0.036) (Figure 3).
We then examined whether a combination of LOH and abnormal protein expression in the same gene was associated with poor differentiation. The results showed that, for ATM, combined LOH and abnormal expression was not associated with tumour grade (Table 1). However, although the number of cases showing both LOH and abnormal expression in the same gene in each pathological grade was small, for BRCA1 and, to a lesser extent, TP53, these combined abnormalities were frequently seen in association with a high tumour grade (P=0.0003, P=0.016, respectively). These results are consistent with the idea that several important DSB checkpoint/repair genes could be inactivated by different mechanisms, and that tumours with LOH and/or abnormal expression in the same gene are more likely to exhibit poorer differentiation.
The demonstration of a significant association between tumour grade and abnormality, either LOH or abnormal expression, at each DSB checkpoint/repair gene prompted us to ask which gene played the most critical role. Logistic regression analysis was performed to resolve this issue statistically. Since the age at the time of tumour onset is an important determinant for tumour grade (Jones and Laird, 1999; Bogdani et al, 2002), we included the patients' ages in this multivariate model. The analysis showed that LOH at BRCA1 (P=0.02) and abnormal expression of BRCA1 protein (P=0.03) were the only two factors significantly and independently associated with poor pathological grade (Table 2 ). This identification of the unique importance of BRCA1, however, did not exclude a joint effect of BRCA1 and other functionally related genes, and we therefore examined whether abnormalities in ATM or TP53 conferred an additional risk in cases in which BRCA1 was abnormal. The result of logistic regression showed a more significant association with high grade in tumours with abnormalities in BRCA1 and ATM and/or TP53 (P=0.001) than in tumours with BRCA1 abnormalities alone (P=0.09) (Table 3).
Support for our hypothesis that abnormalities in individual genes in the DSB checkpoint/repair pathway might act in combination came from the observation of a significant association between poor tumour differentiation and an increased number of these genes showing LOH. As shown in Table 4, 62% of grade I tumours showed no evidence of LOH at ATM, BRCA1 or TP53, whereas all three genes were intact in only 5% of grade III tumours (P=0.002). Furthermore, the results of the Mantel-extension χ2 test for trends confirmed the finding that an increased number of genes showing abnormal expression correlated with poor tumour differentiation (P=0.026). However, since breast cancer always displays a high degree of intratumour heterogeneity in each individual tumour, it was possible that our finding of abnormal protein expression based on the overall abnormal frequency described above might fail to take into account intratumoral differences in protein expression. To exclude this possibility, we used serial sections to analyse the same region for the expression and subcellular location of the proteins of interest. Two typical examples are shown in Figure 4, in which a low-grade tumour displayed strong nuclear staining for ATM and BRCA1 and no TP53 accumulation, while a high-grade tumour displayed abnormal expression of all three proteins.
The rationale for a mutator contributing to the genomic instability that leads to cancer progression is that, instead of being a single-gene disease, cancer is caused by aberrations arising from a complex interconnecting network of multiple regulatory genes involved in normal growth control processes and the maintenance of genomic stability (Orr-Weaver and Weinberg, 1998; Loeb and Loeb, 2000). This rationale supports our approach of simultaneously examining the role of three important genes (ATM, BRCA1 and TP53) involved in the DSB checkpoint/repair pathway. Although abnormalities of individual DSB checkpoint/repair genes or proteins have been reported (Waha et al, 1998; Wilson et al, 1999; Angèle et al, 2000), almost all of these have been studied in isolation without considering the common pathway involved. In contrast, in the present study, using a largely homogeneous group of 74 early-onset IDCs and a multigenetic model, we have evaluated the tumorigenic contribution of LOH and/or abnormal expression of these genes. In terms of methodology, we selected intragenic markers and used laser capture microdissection for our LOH analysis to provide an unbiased estimate of the genetic status of these genes in cancer cells, and our careful examination of protein expression based on serial sections excluded the possibility of an effect of intratumour heterogeneity. The results (Figure 2) showed that loci harbouring ATM, BRCA1 or TP53 displayed a high frequency of LOH in low-grade tumours, suggesting that genetic deletion of these DSB checkpoint/repair genes frequently occurs during the early stage of breast tumour. This is consistent with the results of our previous study (Shen et al, 2000), which suggested that breast cancer progression is driven by DSB-initiated chromosomal instability. In addition, our immunohistochemical study (Figure 3) showed that high-grade tumours tended to have an increased frequency of abnormal expression of these three proteins and, in particular, that abnormal BRCA1 expression was related to poor differentiation (Figure 3), supporting an important contribution of DSB checkpoint/repair genes to breast cancer pathogenesis.
The present study also examined whether combined LOH and abnormal expression occurring at the same gene could contribute to the pathogenesis of breast cancer, and our findings for TP53 and BRCA1 seem to be in line with this hypothesis (Table 1). However, different mechanisms are involved in the effects of combined LOH and abnormal expression of TP53 and BRCA1. In sporadic cancers, LOH at the TP53 locus is usually accompanied by somatic mutation at TP53 (Yang et al, 1997; Kurose et al, 2002), leading to abnormal accumulation of this protein. Thus, a high frequency of poorly differentiated tumours showing both LOH at, and aberrant expression of, TP53 can be explained mechanistically. In contrast, BRCA1 mutations are rare in sporadic breast cancer, suggesting that BRCA1 is inactivated by nonmutational mechanisms (Papa et al, 1998), since epigenetic mechanisms (e.g. promoter hypermethylation), manifested as abnormal expression, have been shown to be involved in abrogating the function of certain tumour suppressor genes (such as BRCA1), which are already targeted by LOH (Dobrovic and Simpfendorfer, 1997; Jones and Baylin, 2002). Our finding that combined LOH and abnormal expression in BRCA1 was associated with a higher tumour grade therefore re-emphasises the importance of the different mechanisms that may affect expression, and this finding is of particular tumorigenic importance in sporadic cancer, as the frequency of somatic mutation in many tumour suppressor genes involved in regulating genomic stability is extremely low. In the present study, we also found that LOH was more frequent than abnormal protein expression at ATM, BRCA1 and TP53. One possible explanation for this is that expression of the remaining allele might increase to compensate for the loss of expression due to the deleted allele (Liao et al, 2003).
The observation that the only gene of the three tested found by multigenetic logistic regression analysis to be independently associated with tumour differentiation was BRCA1 (Table 2) is consistent with the tumour spectrum observed in familial cancer syndromes caused by mutation of ATM, BRCA1 or TP53, with only BRCA1 being specifically associated with familial breast cancer (Holt et al, 1996). However, the present study showed that a joint effect of abnormalities, either LOH or abnormal expression, in BRCA1, ATM and TP53 is also an important factor associated with poor differentiation (Tables 3 and and4).4). This effect can be explained by known mechanisms involving interactions between BRCA1, ATM and TP53 (Xu et al, 1999; Gatei et al, 2000; Wang, 2000; Khanna and Jackson, 2001), including (i) ATM serves as the upstream sensor and, upon DSB formation, phosphorylates BRCA1 and TP53 to trigger DSB repair and cell cycle regulation and (ii) for breast cancer formation, breast epithelium cells that are genomically unstable because of defective BRCA1-associated repair also need to undergo checkpoint inactivation (such as ATM or TP53) in order to escape checkpoint surveillance. More importantly, our demonstration that defective DSB checkpoint/repair genes in a common functional pathway may act together, leading to poor differentiation of breast cancer, is consistent with recent evidence suggesting the joint contribution of different genes in disease aetiology and cancer development. The combination of heterozygous abnormalities in different, but functionally related, genes is known to play a causal role in the pathogenesis of certain genetic syndromes (Cressman et al, 1999; Balmain, 2002). Furthermore, support for a joint carcinogenic effect comes from previous observational studies. For instance, there is a trend towards an increased risk of breast cancer in women harbouring a greater number of putative high-risk genotypes of oestrogen-metabolising genes or nonhomologous end-joining genes (Huang et al, 1999; Fu et al, 2003). Our findings provide additional support for the possibility of a joint effect of defects in different genes and emphasise the need to examine the whole tumorigenic pathway to obtain a better insight into the molecular changes involved in cancer development and progression.
We thank Professor Bruce AJ Ponder of the Hutchison/MRC Research Centre of the University of Cambridge and Professors Chien-Jen Chen and Su-Ming Hsu of the National Taiwan University for their critical reviewing of the manuscript.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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CPSI is a leading provider of healthcare solutions and services for community hospitals plus other healthcare systems and post-acute care facilities. Founded in 1979, CPSI's family of companies – Evident, LLC, TruBridge, LLC, Healthland Inc., and American HealthTech, Inc.
Our combined companies are focused on helping improve the health of the communities we serve, connecting communities for a better patient care experience, and improving the financial operations of our customers.
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TruBridge focuses on providing business, consulting, and managed IT services along with their RCM product Rycan, providing revenue cycle management workflow and automation software to hospitals, other healthcare systems, and skilled nursing organizations.
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Produced by Crossnodes, an internet.com site, the briefings contain an overview of the technology and other information you need to know before purchasing and implementing a specific technology.
CIN has archived nine Crossnodes items under the Great Docs section of our site, in a category we're calling Technology Briefings. There you'll find Crossnodes Briefings on firewalls, WLANs, network storage, gigabit NICs, network diagnostic tools, network management software, network security suites, wireless LAN connections, and e-commerce servers.
(Great Docs, as you may or may not know, is CIN's repository for all sorts of documents ofr interest to CIOs, including surveys and studies, IT policies for members' companies, archived reports and other information. Find the link on the lefthand navigation bar, or


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- W.R. Akeson,
- A.H. Freytag and
- M.A. Henson
Poor emergence of sugarbeet seedling under moisture, temperature, and impedance stress conditions can limit satisfactory stand establishment. The objective of this study was to permeate seeds with dilute acid, growth regulators, and other chemicals, alone and in combinations, and to determine the effect of these treatments on emergence under favorable and unfavorable conditions. Commercially processed seed was treated with 1.0 N hydrochloric acid, propylene, ethylene, 10 and 100 ppm gibberillic acid (GA3), 10 and 100 ppm kinetin, 10 and 100 ppm 6-benzyl-aminopurine (6-BA), 1% sodium hypochlorite, 0.01 M 6.7 and 11.4 phosphate buffers, and fermentation, alone and in combination.
Effects of treatments on emergence of 'GWH 58' were evaluated in laboratory tests with combinations of moisture, temperature, and impedance stress and in field trials in 1977, 1978, and 1979. Laboratory tests showed that the acid treatment stimulates emergence under moisture and temperature, but not impedance stress conditions. The growth regulators - GA3, kinetin, ethylene, and propylene stimulated emergence under combined moisture, temperature, and impedance stress. Under these conditions, acid and growth regulator treatment effects were additive.
In field tests, the growth regulator seed treatments stimulated emergence only in combination with acid pretreatment. The acid + 100 ppm GA3 treatment gave the most consistent positive results (significant at 0.05 in 69% of trials) and greatest average improvement in emergence (9.3% of seeds planted) over the control. Significant improvements in field emergence were also obtained with acid + 10 ppm GA3, acid + 10 and 100 ppm kinetin, acid + 10 and 100 ppm 6-BA, and acid + 100% propylene. Combinations of growth regulators gave no improvement in emergence over that of the individual growth regulators. The acid seed treatment improved emergence only under moisture stress conditions. Cultivars differed in their response to acid + 100 ppm GA, and acid + I00 ppm kinetin treatments in laboratory and field emergence trials conducted in 1980.Please view the pdf by using the Full Text (PDF) link under 'View' to the left.
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All posts tagged "Irregular Periods and Pregnancy"
How To Know You're Pregnant with Irregular Menstrual Cycle
Many women face problems of irregular menstrual cycle (periods). However, this hardly disrupts the normal life; it is a cause of worry when you are trying to get pregnant. Irregular or abnormal ovulation's make up to 40%...
- Posted May 15, 2014


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425 dxnews information
425 DX News issue #1353
DXCC prefix: F - FranceFrank ON4KZ, Tim ON5HC, Patrick ON7PQ and Francis ON8AZ will be active as TM7U from Saint-Nicolas Island (EU-094) on 21-26 May. They will operate CW, SSB and digital modes on 80-10 metres. QSL via ON8AZ. More information and logsearch on TM7U.be. [TNX rsgbiota.org]
information from: http://www.425dxn.org


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Partner With 123Relocation.com!
You are invited to partner with 123Relocation.com, and turn our high quality visitors and customer focused services into revenue for your business. 123Relocation.com features cities and towns located in over 1500 locations throughout the United States and our high visibility ensures that our message (and yours) reaches families going through the relocation process.
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Heavner's Water Trail Paddle Guide
Going on one of our Downriver Trips and don't want to miss your pick-up location?
Going on one of our Downriver Trips and don't want to miss your pick-up location?
See where you are and where you are going on your computer, tablet, or smartphone.
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See the exact location of your pick up point and where you are in relation to it.
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See footage of pick up sites, turns, and portages from the paddler's perspective embedded in Google Earth videos.
Select the Heavner location, then the Pick Up Point, to view site-specific videos and maps.
- 1: Central Park – Milford
- 5: East Boat Launch
- 2: Group Campsite
- 6: Heavner 2 (Kensington Dam)
- 3: Dawson Road
- E: Riverbend
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- F: Placeway
F: Placeway
Click 'View Larger Map' and to see where you are in relation to the Pick Up Point


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The Most Wanted
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Focus on the item you've purchased and/or used. What would you have liked to have known before purchasing?
For non-product related issues - like delivery and customer service - use the contact us form to get in touch.
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text: Sisley Supremÿa Cream: An Ultra High-End Wrinkle Buster After seven years of research, Sisley is launching a $750 night cream called Supremÿa. By Matthew W. Evans on August 14, 2009 It may not seem ideal to launch a $750 night cream in this economy, but after seven years of research on a new product called Supremÿa, upscale cosmetics brand Sisley says the timing is right."Some people say it's not a good time, but we will see," Arnaud Naintré, president of Sisley Americas, said of the launch of Supremÿa, which is due in September. The 1.7-oz. pump bottle is designed to last three to four months. "We are ready to launch it after seven years," he said, adding, "I think we'll have a very good answer from our customer."He noted that the amount of research and development and the type of ingredients that went into the product are the factors that eventually determined the price of Supremÿa.The antiaging facial skin cream contains a patent pending ingredient called Phyto-Complex LC 12 and is designed to reach DNA in the skin in order to help skin cells regenerate. The complex comprises four botanical active ingredients and claims to protect DNA so that genetic aging is delayed."We're now reaching the DNA level to alter a natural process," said Naintré, who added that treatment is the firm's core business.Executives of the family-owned, privately held firm, which is based in Paris, contended that 70 percent of the appearance of aging is caused by external factors, such as UV damage, while 30 percent of aging is genetic. Supremÿa is designed to combat both in all skin types."This stands alone as our flagship product," said marketing and operations manager Dean Robert, "and it will be our flagship product going forward. The value is in the performance of the product."The intended benefits of the cream, which is designed to be used nightly, are to extend of the lifespan of existing skin cells and to boost the skin's own regeneration process, according to executives. These benefits are supposed to then result in wrinkle filling, skin plumping and enhanced skin radiance and hydration.In testing of the formulation, Sisley found that over eight weeks of nightly use, the appearance of wrinkles declined by 11 percent. Also, in vitro testing showed that over a nine-day period, skin explants treated with the Supremÿa formulation appeared to age at half the rate as untreated explants.Supremÿa will be launched at Sisley's 100 points of sale in the U.S. This highly limited distribution network includes Neiman Marcus, Saks Fifth Avenue and Bergdorf Goodman stores. There are also plans for launches at a few Nordstrom locations and Bloomingdale's 59th Street flagship in New York."Our clientele is shopping mainly in those top doors," said Naintré.Industry sources estimate Supremÿa could ring up first-year retail sales of $4 million in the U.S.In order to promote Supremÿa, deluxe samples will be given out to Sisley's "core customers" beginning this month, said Robert. Additionally, visuals are to appear in retailer catalogues."Sampling, service and taking care of our core customer — that's where we invest our [resources]," said Naintré.Internationally, the Sisley brand is carried in 85 to 90 countries, and, in France the brand is carried in some 1,200 doors. To access this article, click here to subscribe or click here to log in. load comments blog comments powered by Disqus


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HR Boot CampTM is an intense training program meant to get you in shape to handle HR challenges and opportunities.
At HR Boot CampTM you will be versed in and explore key areas of HR. Each of the four one-day Modules will provide information, training and exercises in key strategic areas. HR Boot CampTM is meant to provide training and understanding to those that are new to or less experienced in HR and those that deal with HR matters besides their regular duties.
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A very popular pool stick, the McDermott L16 Lucky pool cue features quality workmanship and good looks at a great price. This black pool cue has beautiful overlay points and a genuine irish linen wrap. The wood to wood joint provides a solid feel for every hit, giving you instant feedback. Take your billiards game to the next level with a new McDermott pool cue today and start winning games tomorrow!
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Are You Making These 5 Online Shopping Mistakes?
Shopping online lets you buy anything you want straight from wherever you are, whether it's at home or the subway. The convenience of not having to search through racks of clothes or shelves of shoes is too great to pass up, especially when you know your size and have shopped online for clothes and shoes before. However, things can get out of hand, and before you know it, you've basically overspent your monthly budget and drained your bank account.
To enjoy online shopping without breaking the bank, we've highlighted some of the worst mistakes online shoppers often commit. We hope that these can help you become aware so that you can avoid doing them yourself.
Shopping without a coupon code
Coupon codes exist for a reason. They're there to help shoppers score discounts and grab the best deals online. And because they lead people to make purchases, coupon codes also let businesses grow a profit. It's a two-way street you can take advantage of. There are many coupon or discount codes available online, and there's a code for everything you can think of that's being sold in online marketplaces.
You've got discount codes for shoes, flower deliveries, food, coats, homeware and even hotel rooms. Voucher sites collect these codes and publish them, giving you a one-stop shop for all the discounts you'll ever need. Some stores also advertise coupon codes for products on their webpages and social media accounts, while others send them out through newsletters. If you're a loyal customer, follow your favourite brands on social media or subscribe to their newsletters so you can get first dibs on exclusive promos.
Not checking the size chart
If you like shopping for clothes and shoes, you're probably aware that retailers and brands often make use of different size charts. Your shoe size may be a 7 in one store, but in another store you're an 8. When shopping online for clothes and shoes, make sure to check the size chart they use to avoid ending up with a too-small or too-big shoe or an ill-fitting dress.
While stores do have a return policy for these instances, it's still a hassle having to have the items exchanged. You'll probably have to pay extra for shipping, too.
Not shopping around
Unless the product you want to buy is an exclusive from just one retailer, it would be better for you to shop around first before hitting the "Buy" button. Take time to shop around online to see which retailer offers the best deal. Use price checkers and price comparison tools to help you see which stores have the item you want and how much they're selling it for. Don't forget to check shipping rates and return policies to help you make an informed decision.
Not reading verified customer reviews
Unbiased reviews from verified customers are a treasure trove of information about a product or service. Most customers will leave a review of a company or product on two occasions: when they're extremely satisfied or happy, and when they're extremely disappointed and upset.
Before buying anything, read reviews on the product or service and the company you're getting it from. Verified customer reviews will give you a better sense of the quality of a product and consumer experience.
Using a debit card instead of a credit card
Credit cards are generally the more secure option to pay for online purchases. They also offer better consumer protection against unauthorized transactions. It's best to use a separate credit card for online shopping.
You can also apply for a prepaid credit card from your bank. Many banks offer MasterCard prepaid cards that may be used for online purchases as well as restaurants and shops that accept MasterCard. You just have to load the card with money before using it. This is a great way to control and manage your online spending.


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Two Sudan rebel leaders arrived in The Hague on Wednesday after surrendering to the International Criminal Court to answer to accusations of war crimes in Darfur, the court said.
"Abdallah Banda Abakaer Nourain and Saleh Mohammed Jerbo Jamus, both suspected of having committed war crimes in Darfur, Sudan, arrived voluntarily this morning at the International Criminal Court," a statement said.
"Both suspects will stay at the location assigned to them by the court until their first appearance before the chamber" Thursday morning, after which they would be free to leave the Netherlands pending further appearances.
The men face three counts of war crimes allegedly committed in an attack on the Haskanita military base in north Darfur on September 29, 2007, that killed 12 African Union Mission in Sudan (AMIS) peacekeepers and injured eight.
The attack was allegedly carried out by fighters of the Sudanese Liberation Army-Unity under the command of Jerbo, and splinter forces of the Justice and Equality Movement led by Banda.
"It is alleged that the attackers, numbering approximately 1,000, were armed with anti-aircraft guns, artillery guns and rocket propelled grenade launchers," said the court statement.
"During and after the attack, they allegedly destroyed AMIS communications installations, dormitories, vehicles and other materials and appropriated AMIS property, including refrigerators, computers, cellular phones, military boots and uniforms, 17 vehicles, fuel, ammunition and money."
A pre-trial chamber had found there were reasonable grounds to believe that Band and Jerbo were criminally responsible for murder, an intentional attack on peacekeepers, and pillaging, said the statement.
Summonses for the men to appear were issued under seal in August last year. Arrest warrants were not considered necessary.
The court said the men would make an initial appearance on Thursday, during which they will be informed of the allegations and of their rights.
A further hearing will be held "within a reasonable time" to confirm the charges against the two -- a requirement before the case can go to trial. Their appearance at this "confirmation of charges hearing" was not obligatory.
The case is the fourth before the ICC involving alleged warcrimes in Darfur.
Arrest warrants are outstanding for Sudan's President Omar al-Beshir, former government minister Ahmed Haroun and militia leader Ali Kosheib.
Beshir rejects the jurisdiction of the ICC, the world's only independent, permanent court with authority to try genocide, crimes against humanity and war crimes, and has refused to hand over his two allies.
ICC judges have turned down a prosecution bid to prosecute fellow rebel chief Bahar Idriss Abu Garda over the Haskanita attack, finding insufficient evidence to link him to the killings. He had also appeared before the ICC voluntarily.
The United Nations says more than 300,000 people have been killed since the Darfur conflict broke out in 2003, when minority rebels took up arms against the Arab-dominated Sudanese government for a greater share of resources and power.
The Sudanese government puts the death toll at 10,000.


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The fuel industry is focused on the sale of heating oil, gas, and other various grades of oil such as kerosene. Auto fuels are broken down into two segments of diesel and gasoline. Recent trends in this industry are focused on increasing the capacity of fuel cells, hydrogen refueling stations, and earning profitability. Private and corporate investments are increasing in global regions such as Russia, South Africa, and Asia Pacific. Current revenue earnings are expected to rise from $1 billion to $2 billion globally in recent times. A series of fuel company logos from the top innovators and generating fuel cell organizations in the United States are listed below.
Current independent power producers and energy companies have been forming partnerships with other utility businesses to increase services and revenue. Fuel cell based vehicles have been increasing interest among the public and investors with advances made surrounding fuel cell vehicles.
Here are the secret tactics I used to build my website traffic to over 2 million monthly visitors and grow my email list to over 100,000 subscribers:
9 Secrets to Increasing Website Visitors and Email Subscribers
9 Secrets to Increasing Website Visitors and Email Subscribers


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This product is no longer available.
You can try these similar gifts instead.
Milwaukee Brewers Golf Headcovers
You will definitely hit a hole in one with the resident Milwaukee Brewers fan when you give him these handsome golf club head covers. These eye-catching head covers are adorned with the Brewers official logo.


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Manmarziyan 1st July 2015 Written Episode Update
The Episode commences with Saral bringing Radhika. Sam tells Samrat that she desires to spend her everyday living with Arjun and asks his view. Samrat suggests concept is incredibly negative, marriage way is extended, it will eventually hurt a whole lot, issues will appear at just about every switch, but if lifetime husband or wife is sweet, then its enjoyment to resolve the existence troubles jointly. She smiles. Piyali suggests no relation is simple. Samrat recalls Nandini. Piyali says marriage is relation is 2 people and even Lord has no authorization to come back concerning, I've hurt you a large number and hope Arjun erases the many bad Reminiscences. Sam says I love you mum. They cry and hug.
Saral likes the house and smiles. He asks Radhika to come, will she continue to be here till early morning or stroll ahead. Arjun feels bad observing Saral having arrogantly with Radhika. Piyali asks Arjun to keep Sam satisfied and recall she is his manager, and asks his motive. He claims to choose care your business. She laughs. He claims I can get opportunity to devote time with you and Samrat, this enterprise is great deal for me. Piyali claims now I see what Sam finds in you, don't get in touch with me mummy, Piyali appears to be excellent to listen to from you.
Sam introduces Mala to Piyali. Mala suggests Radhika tells lots about you. Piyali asks Radhika how is she. Radhika states I m high-quality. Sam claims This really is Radhika's fiancé, Saral. Piyali asks Radhika about her engagement, she did not tell them. Saral appears at her. Saral holds her hand and can take her to facet. He claims she altered after coming Mumbai. Arjun looks at them. Saral claps for her and taunts her. Radhika goes. Arjun gets heart ache observing this.
Samrat compliments Mala that she appears Radhika's elder sister. They smile. Mala states Sam informed that Arjun is going to speak tonight, so we make laddoos and Dahi wada at these situation, You could have designed it proper, get this laddoo from our facet. Samrat and Piyali take a look at each other. Sam tells them about her best friend Neil. Samrat asks Piyali to take pleasure in the laddoos. Piyali asks Saral where is his drink and offers him once.
Arjun seems to be at Samrat's family pic and Radhika appears to be like at him, recalling Nandini. Samrat arrives between and she stops. Arjun recollects the past and suggests aged Recollections don't go anyplace and look ahead to any lifetime's change, once the turn will come infront of us, the previous also comes in our sight. Samrat states deep considered. Saral asks Radhika to return to him. Samrat asks Arjun about his family members, will no person occur below from his household. Arjun states I don't have any spouse and children, I m all by itself Within this entire world. Samrat receives stunned. Saral sees Radhika watching Arjun, and holds her hand tightly twisting. Arjun seems to be at them and retains anger in his eyes for Saral.
Samrat claims I feel All your family members was in London. Saral claims I don't Imagine Arjun is from London, my Pal went to London and speaks English fluently. He asks her to have a consume. She refuses and he insists. Arjun stares at them. She suggests she can get the cold consume. He asks her to test a thing new and taunts her. Arjun receives Nandini's get in touch with and keeps the cellular phone observing Samrat. Arjun suggests the routes which he went via had just darkness and he would not would like to recall now, he has developed up in orphanage and received scholarship to review in London. Samrat says its hard to achieve to achievement on own. Saral touches Radhika and suggests Arjun Sir is seeing as if I m touching his fiancé. Khwaishon pe likhi……..plays……… Arjun angrily leaves. Radhika asks Saral what on earth is he performing, everyone seems to be listed here. Saral says allow them to know the way passionate I m.
Sam asks about Neil. Radhika states he may be chaotic in perform. Sam states whats more imp than me. She asks Neil's mum Prerna. She asks is Neil annoyed with me. Prerna states he received't occur Sam. Arjun talks to Nandini and states he will almost certainly speak with Samrat now. He hears footsteps and finishes the decision. He turns and sees Radhika. Dastaan ek nayi………plays……….. Arjun asks did she depart her fiancé by yourself. She claims she didn't see ghost in healthcare facility, he fulfilled an individual there, you explained Nandini Di, that is she.
Radhika asks why did he lie, she has viewed Nandini there. Arjun sees Samrat coming to them and will get tensed.
Written Update By Prince
Show Info:
Manmarziyan 1st July 2015 Written Update, Manmarziyan 1st July 2015 Written Episode, Written Update of Manmarziyan 1st July, Manmarziyan Written Update of 1st July 2015 Read Story Online.


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Groceries, I suppose
Are you interested in architecture?
Groceries, I suppose
Are you interested in architecture?
Yes, on a superficial level.
Are you watching a football game right now?
Are you going to watch any of the Once Upon a Time repeats?
No, not a fan of that show
Are you hoping everyone will have a happy and healthy New Year?
Oh yes!
Are you going to do anything particularly special this year?
Probably not
Are you still in your night clothes?
Are you feeling pain in your hips?
Are you going to watch the Bach tonight?
Are you going to come back in here after the show?
"Dogs do speak, but only to those who know how to listen." ~ Orhan Pamuk


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Quote by Stendhal
People happy in love have an air of intensity.
Similar Quotes
Life in common among people who love each other is the ideal of happiness.
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Wine is constant proof that God loves us and loves to see us happy.
I knew what my job was; it was to go out and meet the people and love them.
The secret of being happy is accepting where you are in life and making the most out of every day.
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By Thomas Erdbrink
Washington Post Foreign Service
Wednesday, October 13, 2010; 4:07 AM
TEHRAN -- An explosion in an ammunition depot at a Revolutionary Guard Corps base killed 18 people and injured 14, a commander of the elite force said Wednesday.
Yadollah Buali, a deputy commander, told the semiofficial Fars News Agency that ammunition exploded after a base storage facility caught fire Tuesday.
"This is almost the final death toll," Buali said.
It was unclear whether the incident, which took place near the western city of Khorramabad, was an accident or the result of terrorism or sabotage.
Firefighters and rescue teams were dispatched to the scene and the injured were rushed to the hospital, Fars reported. According to the Islamic Republic News Agency, the base was used as a training center.
Last month, a bombing killed 12 people and injured 80 in the ethnic Kurdish city of Mahabad during a parade by members of the army and the Guard Corps. Authorities blamed "anti-revolutionary" militants backed by foreign enemies and said they killed 30 rebels responsible for the attack.


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All too often we as adults lose the wonder of the Christmas story in the midst of its familiarity. It really is an amazing, awe-inspiring, true story! An angel – yes, big, frightening, heavenly being – came and talked to Mary. He told her she was going to have a baby, and she was still a virgin. This baby would be God's Son, the long-awaited Messiah, sent to save the people from their sins. That is a story worth taking time to marvel about and to worship our God because it is true. Experience the wonder of the story through the eyes of your preschooler this week as you reinforce this story through these activities.
HIGHLIGHTS from Sunday…
This week your child learned that Mary was chosen by God for a very special job. She was given this message by the angel, Gabriel, who came to her one day and told her she would have a baby and His name would be Jesus. He would be a very special gift to the world—He would be the Son of God. Mary was frightened, but the angel told her not to be afraid. God was pleased with her and would take care of her. Mary chose to be obedient to God's plan and trust in Him. She said, "I am the Lord's servant. May it be to me as you have said." After Mary thought about these things for a while, she went to visit her cousin Elizabeth and tell her the good news.
Where's It Found?
Luke 1:26‐38
Teaching Truths:
- Jesus is God's son.
- Jesus is a gift from God.
Memory Verse:
"For God loved the world so much that He gave his one and only Son, so that everyone who believes in Him will not perish but have eternal life. "
John 3:16 (NLT)
Here's a simple song to help your little one (and you) memorize this month's memory verse!
3 Fun Family Activities that Remind Us of God's Greatest Gift
1. Check Out a Live Nativity
Take your family to experience a live nativity! Many churches in the area provide an opportunity to see a live re-enactment of the nativity. Find one close to you and take advantage of this interactive learning experience.
2. Christmas Angel Craft
Over the next few weeks, let's make a few ornaments to remind your child of the events of Christmas. This past Sunday, your child learned about the appearance of the angel to Mary. So, let's make a Christmas angel …First grab your supplies: 2 coffee filters, washable markers, a squirt bottle or small glass of water, plastic spoon, and tape. Lay the filters flat and let your child scribble on both of them with the markers. Use the squirt bottle and spray in the middle once or twice and watch the colors spread, usually takes about five minutes. Let the filter dry completely, about 30 minutes. Scrunch the filter in the middle and wrap tape around it for the wings. Fold the second filter in half, wrap together to make a cone and tape it so it is secure. Tape the wings to the back of the cone. Draw simple face on the plastic spoon and insert it into the hole at the top of the cone. Lastly, as your child finds a spot to place the angel on the tree, talk to him about how Mary reacted to the angel by using these questions. Mary had an obedient heart when the angel told her what God wanted her to do. Here's some questions to talk about it afterwards.
- What does God ask you to do today?
- How can you show your obedience like Mary?
3. Toddler Christmas Carols
Incorporate Christmas carols into the time you spend together with your child at bedtime or during the day singing songs together. Take time to talk about the words to songs like Away in the Manger, Silent Night, Hark! The Herald Angels Sing, or What Child is This.
Dear God, Thank you for your wonderful gift of Jesus! We know you sent Him to take away our sins because you love us so much. Help us to love others and share the gift of Jesus to those who don't Him. Amen
The post 3 Mary is Visited by an Angel Activities for Preschoolers: Starting Blocks Playbook for December 8th appeared first on Watermark Community Church Blog.


text: The FM Radio app on my Evo died for some reason and now turns on to station 00.0 and I can't do anything. Does anyone know how I can fix this app or know of another app that will allow me to tune in over the radio stations? All of the "radio" apps I keep seeing and hearing about are for webcasts of the radio programming, and I want to actually use the FM tuner. THANKS!!!


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While not a young adult novel, I was mesmerized by the fascinating history of the Valley. The history of the valley is richer and far more interesting than most of its inhabitants realize. Good for browsing, looking at the pictures, but be careful, you may get hooked into the fascinating reading.
Reed Reads Score: 4


text: Zhejiang Zhengda Air Separation Equipment Co., Ltd. is a collection of various China Other manufacturer and Other factory,we focus on developing high-quality products for OEM markets.Other in China and the world enjoy a high reputation. Zhengda fully implements the office automation (OA), warehouse management system (WMS) flow, and safety production standardization, performs the ISO9001 quality system. Zhengda is leading China PSA equipment to move towards a new era, each Other have passed the first inspection, process inspection, error prevention, and laboratory tests to ensure that each piece of qualified Other sent to customers.


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Nano Skate-shoeEastman Tritan™ copolyester provides excellent mechanical strength through injection molding
Who- Heelys, the skate-shoes with a hidden wheel
What- Nano, an inline footboard that bridges a gap between traditional Heelys and skateboards, aiming to retain the utility of the former, while seizing the toughness and customizability of the latter.
Needs- The Nano needed a material tough enough to endure the hammer they would inevitably get. To appeal to their market, the Nano features swappable lenticular inserts and offer special editions with LED wheels. These artistic qualities require a material that can maintain its clarity over an extended period of time.
"We want something that can be clear, or tinted, but still strong and durable," says Ryan Wills, director of innovation for Heelys. "We need a material that can withstand the impact and rigors of daily use with a reasonable level of wear."
Wills also needed a material with excellent mechanical strength that can be injection molded easily and was BPA free.
Materials- All-Plastics Molding, Inc., of Addison, Texas, recommended Tritan, the BPA free alternative to polycarbonate that's impact-resistant and durable, for the job.
"Tritan is exactly what I was looking for," says Wills. He adds "The whole thing with wheels in and graphic and axles weighs slightly less three pounds. The average wall thickness of the board is about 3.5 millimeters. The chassis is in two pieces with a tongue and groove around the outside and it's screwed together with nine stainless steel screws."
Success- The design of the Nano has already attracted international attention, winning a 2011 iF (International Forum) product design award.


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The Chauvet BeamBar LED Lighting Effect will make a great addition to any lighting system and projects razor-sharp beams of white light for stunning visual effects! Programming the BeamBar to emit intense runway effects is very easy. You also have the ability to build a massive matrix by combining several units using the CBB-6 mounting bracket. Use the Chauvet ShowXpress Pixels software to create eye-catching sweeps and animations in DMX mode; or for complete ease, activate exciting built-in, automated programs.
Chauvet BeamBar LED Lighting Effect Features
What started as a simple string and pedal sales catalog has grown exponentially over the past thirty years. The early days were a time where catalogs didn't have much of a presence in the industry, but what began as a college dorm room operation grew rapidly. In 1986 we moved to a full product offering and 64-page catalog, which over the years has grown to 162 pages. Join the AMS family and get your free catalog now!
Copyright © 1996-2017 American Musical Supply, Inc. All rights reserved. American Musical Supply reserves the right to correct pricing and/or product specifications in the event of typographical errors found in our print catalog or on our website. If a product is listed at an incorrect price due to such errors or because of inaccurate information received from a supplier, American Musical Supply shall have the right to refuse or cancel any orders placed, whether or not the order has been confirmed and your credit card charged. If your credit card has already been charged for the purchase and your order is canceled, American Musical Supply will issue a credit to your credit card account in the amount of the charge.


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Jacob Black
Portrayed by Taylor Lautner
Species werewolf
Spouse(s) Renesmee
Jacob Black is Bella's best friend. He is a Quileute Native American and a werewolf. In Twilight, Jacob plays the minor role of a forgotten childhood friend of Bella's, and he develops a crush on her. In an attempt to learn more about Cullens, Bella flirts with Jacob, and he tells her tribe legends about "the cold ones", or vampires. After Edward leaves Bella in New Moon, she spends much of her time with Jacob. Though she only considers him a friend, Jacob falls in love with Bella. Although he spends most of his time in Eclipse trying to win Bella, in Breaking Dawn he imprints— an involuntary process in which a werewolf finds their soul mate— on Bella and Edward's daughter, Renesmee.


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Ceremony and Tickets
Guest tickets for the ceremonies in the Cathedral are £13.50 and include strawberries and a glass of Prosecco or a soft option of your choice for you and your ticket paying guests. This will be available at Bishop Grosseteste campus from 11:00am – 6:00pm. There is no charge for graduands.
Due to the limited number of seats available in the Cathedral, we are only able to initially offer each student three guest tickets.*
The design of the Cathedral might mean that you may not be able to see all the proceedings all of the time from where you are sitting, so large visual screens are placed in the Cathedral to allow guests a clear view, even from the back of the Nave. It is considered that the ceremony may not be suitable for young children. If you are bringing children to the ceremony, we politely ask that you try to keep them quiet and seated throughout the ceremony, in respect of this special day.
Please note that due to health and safety regulations children will require their own ticket and will not be able to sit on laps. The graduation ceremony will last approximately one hour.
In addition to the ceremonies in the Cathedral, there will be a link on the BGU Graduation Website to live streaming, so that ceremonies can be viewed online all over world!
Additional Tickets
Availability of additional tickets over and above the 3 guest tickets detailed above cannot be guaranteed or confirmed until all the applications have been processed. We will email your student account on Tuesday 23rd May with details of availability and how to apply with the online ticket website. The website will not go live until the Friday 26th May. Subject to availability, this system will issue available extra tickets on a first come first serve basis.
Please note: All tickets will be included in your information packs and these will be posted to your home address during the second week of July.
Applications for tickets should be made via the link provided on the Registering & Ticketing page by Friday 12th May or the link below.
Please note: We regret that under no circumstances will late applications be considered.
Booking Tickets and Payment
Please click here to log on to the Ede and Ravenscoft site to order your ceremony tickets, gown and any photographs you may require. You must book your graduation tickets by the end of Friday 12 May, even if you think you might not pass the course. Gowns can be booked at a later stage no later than 21 days before the ceremony.
Any queries about graduation can be made at the Graduation Office in Room 5a in the Skinner building, or over the telephone on 01522 585635 from Monday to Friday, between the hours of 9.00am and 4.30pm.
If you have not received a formal invitation through the post, please contact the graduation office on 01522 585635 or email email@example.com
*In the very unlikely event of 100% take up on the initial registration the third ticket purchased will be refunded and the surplus tickets re offered on a first come first served basis.


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Bogus bankers are targeting elderly people across Harrogate in a scam to get them to part with their pin numbers and bank cards.
Police are now warning people in Harrogate to be wary after they received four reports of attempted frauds over just two days last week.
In two cases the fraudsters actually visited their victims, stealing large amounts of cash after tricking them into passing on their bank details.
"Elderly people have been duped by these despicable people who appear very plausible and genuine," said Det Sgt Hannah Wright, of Harrogate CID.
"They are smartly dressed and try to confuse people into parting with information.
"If you have elderly relatives or friends, please urge them not to hand over their bank details of cards and reassure them that it's not impolite to refuse to answer your door or hand over information."
Police say officers in Harrogate received four reports of conmen phoning elderly residents to say there is a problem with their standing orders or that a fraud has taken place on their account.
A short time later, someone else calls them claiming to be from their bank and that they are calling to sort the problem out.
They then try to trick the elderly person into parting with their bank details, including their pin number.
"We want to warn people not to answer their door to anyone who they do not know," said Det Sgt Wright.
"If anyone calls at your door or promises to call at your home to collect your cards, do not answer the door and call the police on 999 immediately. If you believe you have been targeted in a similar way over the past few days, please call the police on 101.
"We can't stress how important it is not to give your bank details to anyone. And to never hand your bank or credit card to anyone unless you are inside the premises of your own bank.
"A bank would never call to pick up bank cards in person or ask for your pin number."
Police are investigating and are asking anybody who has seen anything suspicious in Killinghall, Burn Bridge, Crescent Road or the Royal Parade area of Harrogate, to get in touch.
"These people must be stopped," said Det Sgt Wright. "If anyone else has been the targeted by them, I urge you to contact the police without delay.
"Even if they did not succeed, please tell us as we need to gather as much information about them as possible."


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I'm experimenting with Server for NFS (W2k3 R2) and have gotten as far as getting mount failures from the Unix client.
I've configured Name Mapping.
I've set the NFS share to allow anonymous access.
I've allowed access to all machines in .maphosts.
I've set 'everyone' to full control on the folder (ntfs and share.)
The message from the client is either: "no such file or directory" or "nfs mount: RPC: Program not registered."
The syntax on the client to mount is "mount -F server_name:/share mountpoint."
Anybody have any thoughts?
Collapse -
help regarding NFS
hi...i need help regarding NFS....is there any source code available on net for data transfer available on net...plz reply back soon...thanx