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Media reports say Iran has shut down the offices of an exiled former Afghan warlord with stated opposition to Afghanistan's new interim government.
Iranian newspapers said the move against Gulbuddin Hekmatyar -- who has lived in Iran since 1996 -- came after officials determined that he acted against the interests of Iranian national security.
Gulbuddin Hekmatyar has rejected the international accord that established Afghanistan's new government, saying the pact negotiated in Germany late last year amounts to a U-S-imposed government.
Reports last week said Iran was considering cracking down on Mr. Hekmatyar and expelling him from the country after he began speaking out against the Kabul government. He also told Reuters (news agency) that he controlled armed dissident factions inside Afghanistan ready to resist the new Kabul government.
In the past month, the Bush administration has repeatedly accused Iran of trying to destabilize Afghanistan's fledgling government, either by harboring its opponents or by supporting anti-government figures inside Afghanistan. Iran has flatly denied the charges.


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Category: Stock Graphic, Illustrations
Stock Graphic >> Illustration - Watercolor Christmas Decorations 205191 by Corner Croft @ CreativeMarket
File Size: 24.47 MB; DPI: 300 DPI; Dimensions: 5 x 5.5 in.
26 Hand painted watercolor Christmas Decorations - poinsettia, holly berries, Christmas stockings, pine cone and pine needles, gift boxes, ivy leaves for making Christmas greetings cards, wedding invitations and Christmas decor.
26 High quality images png , 300 dpi with transparent background.
The images size varies, typically snowy holly and red poinsettia 12 cm x 15 cm (5"x5.5")
The digital clip art comes with the Simple License. You are not permitted to re distribute as it is.
For commercial mass printing or any commercial digital production please purchase Extended Commercial Use License at https://creativemarket.com/chengjing/163445-Extended-Commercial-Use-License
Corner Croft retains all copyrights to the digital files.
Keywords: hand painted, watercolor christmas decorations, poinsettia, holly berries, christmas stockings, pine cone and pine needles, gift boxes, ivy leaves, greeting card, wedding invitations, watercolor clipart, watercolor clip art, watercolor christmas, christmas deco, pine cones, pink needles, watercolor, red poinsettia, corner croft, hand drawn.

subset: commoncrawl


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Barney's Auto Service
Review for Barney's Auto Service
Richard K. on 03/02/2013
"Good service wrong quotes"
As always the service was great. Barney is a great mechanic. I was very disappointed that I received 3 different quotes. When the vehicle was done I had to pay an amount that wasn't even said in the first 3 quotes. The quotes were 960, 1177, and 1077. I had to pay 1145.13.
Vehicle: Dodge Charger
Service Date: 03/01/2013
Review Created: 03/02/2013 05:26 AM


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In quantitative data analysis you are expected to turn raw numbers into meaningful data through the application of rational and critical thinking. The same figure within data set can be interpreted in many different ways; therefore it is important to apply fair and careful judgement.
For example, questionnaire findings of a research titled "A study into the impacts of informal management-employee communication on the levels of employee motivation: a case study of Agro Bravo Enterprise" may indicate that the majority 52% of respondents assess communication skills of their immediate supervisors as inadequate.
This specific piece of primary data findings needs to be critically analyzed and objectively interpreted through comparing it to other findings within the framework of the same research such as organizational culture of Agro Bravo Enterprise, leadership styles exercised, the levels of frequency of management-employee communications etc.
Moreover, literature review findings conducted at the earlier stages of the research process need to be referred to in order to reflect the viewpoints of other authors regarding the causes of employee dissatisfaction with management communication and integrating secondary data within discussions and analyses in a logical and unbiased manner.
Let's take another example. A dissertation exploring the impacts of foreign direct investment (FDI) on the levels of economic growth in Vietnam for a specific period of time with the use of correlation method, where FDI and GDP have been specified as the two variables produces a correlation coefficient of 0.9.
In this case simply stating that there is a strong positive correlation between FDI and GDP would not suffice; you have to provide explanation about the manners in which the growth on the levels of FDI may contribute to the growth of GDP by referring to the findings of the literature review and applying your own critical and rational reasoning skills.
A set of analytical software can be used to assist with analysis of quantitative data. The following table contains advantages and disadvantages of three popular quantitative data analysis software: Microsoft Excel, Microsoft Access and SPSS.
|Excel Spreadsheet
|· Cost effective or Free of Charge
· Can be sent as e-mail attachments & viewed by most smartphones
· All in one program
· Excel files can be secured by a password
|· Big Excel files may run slowly
· Numbers of rows and columns are limited
· Advanced analysis functions are time consuming to be learned by beginners
· Virus vulnerability through macros
|Microsoft Access
|· One of the cheapest amongst premium programs
· Flexible information retrieval
· Ease of use
|· Difficult in dealing with large database
· Low level of interactivity
· Remote use requires installation of the same version of Microsoft Access
|· Broad coverage of formulas and statistical routines
· Data files can be imported through other programs
· Annually updated to increase sophistication
|· Expensive cost
· Limited license duration
· Confusion among the different versions due to regular update
Advantages and disadvantages of popular quantitative analytical software
My e-book, The Ultimate Guide to Writing a Dissertation in Business Studies: a step by step assistance offers practical assistance to complete a dissertation with minimum or no stress. The e-book covers all stages of writing a dissertation starting from the selection of the research area to submitting the completed version of the work before the deadline.
John Dudovskiy


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4420 Collins Rd, Rochester, Michigan 48306 Copyright 2013. Paint Creek United Methodist Church. All Rights Reserved.
The annual rummage sale at the Paint Creek UMC will be Friday, March 18, 9-5 and Saturday, March 19, 9:00-1:00. Early birds on Friday at 8:00 AM can enter for a $4.00 charge. Saturday is bag day (we supply) and charge is $5.00/bag.
If you have items to donate, please drop them off starting on Monday, March 14 through Wednesday, March 16 between 10:00 AM and 5:00 PM.
Please no computers, printers, tvs or textbooks. We do accept clothing/shoes, furniture, household items, linens, tools, toys, books and appliances (if they work).


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Considered as the capital city of the O&G Industry, Houston is the place to be for service providers. Even in those difficult times of low oil price, Houston and the Gulf Of Mexico remain hot spots where the latest innovations are being tested on strategic issues. ESTIMAGES wishes to take part in this innovation trend by offering new geostatistical applications and by developing or strengthening collaborations with complementary activities such as software companies.
With this new office, ESTIMAGES' goal is to respond to American clients' needs for geostatistical QC/filtering of seismic data, velocity modeling and related uncertainties. Indeed, when mastered, geostatistical techniques allow to reduce risks associated with the exploration and production phases, facilitating seismic interpretation and reducing depth uncertainties for well planning. Based on a successful presence in Norway and Australia, Houston will serve as a launch pad for the spread of ESTIMAGES expertise in the Americas.
To drive this expansion, Lara SATGE , Business Development Geoscientist left our Paris office end of 2016 and started the activity from our Sugar Land based office beginning of January. She will be listening to technical challenges faced by the Oil&Gas Professionals .


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Fundraising Brick offers you services that go above and beyond those of our competitors. Our goal is to help you plan a successful buy a brick or tile fundraising campaign, and to ensure we do just that, we provide our customers with a collection of complimentary special services. Many of our competitors charge extra for these specialty service upgrades; however, we provide them at no additional cost to because we are committed to providing our customers with the absolute best experience possible.
When you choose us, you choose product excellence and reliability. We produce laser engraved bricks that you'll be proud to use in your brick fundraising project, but that's just the beginning. We also support the project itself—every step of the way. Our wide range of services and resources include:
And why do we go above and beyond you might ask? Because we know one thing to be true. If we work with your organization on a buy a brick or fundraising tile campaign that produces great results, not only are you likely to come back to us the next time you have a fundraising project, but you will be sure to tell your friends, family and colleagues about your positive experience. And rather than spending our money on expensive advertising, we'd rather provide you with a superior experience and in turn, rely on your word of mouth "advertising" to get the word out about the Fundraising Brick advantage.
Our fundraising team is available to you at any time for guidance and support. We realize that most fundraising project leaders are volunteers who can't always plan and execute these projects within normal business hours. That's why our highly experienced team is available 24 hours a day, seven days a week – yes, including nights and weekends—to assist you with your fundraising project. Only Fundraising Brick can offer you and your organization this standard of 24-hour service and personal dedication.


text: My phone entries are all area code first, then 7 digit phone number. I've noticed that it places a 1 in front of every number I dial out. I know I can turn this off, but is it necessary to do this by Sprint? I know my ATT phone didn't need the 1 in front, area code by itself worked fine, even on long distance calls. I also noticed, if I just type the number in, no area code ... it looks at it like it's a totally new number than the one in my phone. My last phone wouldn't care if I put area code first, it would pick up that contact and show that picture. Any thoughts on this?


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April 11, 2007
Highlighting the environmental pitfalls of harnessing "green" energy, Los Angeles Mayor Antonio Villaraigosa's push to import nonpolluting power could require building power lines and transmission towers through a national forest, two desert wildlife preserves and a rustic hamlet, says the Los Angeles Times.
As part of the mayor's plan to make Los Angeles "the greenest city and cleanest city in America":
- He wants to build an 85-mile-long "Green Path" energy corridor designed to bring solar, geothermal and nuclear power from southeastern California and Arizona.
- The mayor is also pushing aggressively for 20 percent of the city's power to be renewable by 2010.
- In addition, officials have chosen not to renew a contract with a Utah coal plant that provides more than 40 percent of the city's power; that pact will expire in 2023.
Many preservation and community groups have condemned the mayor for a plan that they say would destroy priceless vistas, natural areas and wildlife corridors. Justin Augustine of the Center for Biological Diversity recently wrote Villaraigosa a letter saying that not only was such energy consumption not 'green,' but unacceptable under any name. The ends cannot justify the means, he said.
Further, the anger over the proposed route underscores challenges nationwide over how to ship wind, sun and steam power from remote rural reaches to booming urban centers.
"People do not like the way power lines look," said George Douglas, spokesman for the National Renewable Energy Laboratory. The chances it's going to happen are zero, because nobody's going to build the transmission lines. They're great big things that cost a lot of money, and people don't like them. They are unsightly -- there's no two ways about it -- and when you build them, they definitely disturb the land."
Source: Janet Wilson, California 'Green' project makes critics see red," Los Angeles Times, April 9, 2007.
Browse more articles on Environment Issues


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Argentina 2015 SUBBED DVDRip x264-RedBladeOct 26
2 nominations. See more awards »
Plot: We are very happy because the Zonda film, Folklore Argentine Carlos Saura was screened at the opening of Chungmuro International Musical Film Festival (in the city of Seoul, South Korea), with the presence of the Executive Producer Marcelo Schapces, Ambassador of Argentina Jorge Roballo and a great reception from the public.
The film represents one of the biggest attractions of the festival. July 8 morning there will be another function in Megabox Cinema in downtown Seoul and Saturday 9 will be shown in a drive prepared for this event.
Here we share some photos of what was yesterday Zonda, Argentine Folklore Festival at the opening.
Genre: Documentary | Musical
IMDB Rating: 6.9/10 From 66 Users
Directed by: Carlos Saura
Starring: Pedro Aznar, Juan Falú, Marian Farías Gómez
Release Name: Argentina.2015.SUBBED.DVDRip.x264-RedBlade
Size: 756 MB
Video: MKV | 720×388 | 1204 kbps
Audio: Spanish | AAC | 128 kbps
Runtime: 1h 25min
Subtitles: English (Hardcoded)
Samples: Video @ #1 – #2 – #3
Links: iMDB | Trailer | NFO | Torrent Search
Download: NiTROFLARE – UPLOADED – RAPiDGATOR – UploadRocket
Next Post :2DArtist – October 2016


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The Vistula is the longest river in Poland and traverses the entire length of the country before emptying itself into the Baltic Sea. The entire river is almost 1000 km long and reaches all the way from south to north. Along this river, you will find banks that are peppered with shrubs and trees with flowers in every color you can imagine. However, the icing on the cake remains some of the most dramatic palaces and castles along the way. Here's a list of some of the most amazing castles that you absolutely must not miss.
1. Malbork Castle
This magnificent castle is located 150 km north of Torun and is a stark reminder of the great skills of the Teutonic Knights. This castle is situated next to the River Nogat, near the Vistula delta, and evokes a strong sense of solemnity, mysticism, and romance. It consists of spires, turrets, gables, and towers and is built on several levels, rightfully earning its place among the largest brick castles in the continent. Inside the castle, you can find a winding maze of corridors, chapels, courtyards, and murals telling tales of the bravery and valor of the Teutonic Knights.
2. Wawel Castle
You will find this 14th century castle located on a hill towards the south of Poland, right after a sharp bend in the river just outside Krakow. This is a Gothic castle, as you can tell from the whimsical structure that consists of a few buildings. The inside of the castle has elegant rooms, completed with massive tapestries and Renaissance paintings you absolutely cannot miss. There is also a cathedral next door with beautiful sculptures. Right above the main doorway, you will also find a set of bones that are believed to be the skeleton of mammoths and which have been hung there as a sign of good luck. This cathedral is where many Polish kings have been crowned and are now buried.
3. Torun Castle Ruins
The walled city of Torun is located along the Vistula river 200 km further on from Warsaw. Once you enter the city, you feel as if you are taking a trip in history. Torun revolves around the life of Copernicus, the historical astronomer and mathematician. It is also a reminder of how powerful the Teutonic Knights were, who were responsible for building and developing the city. They controlled the city between the 13th and 15th centuries. Their most famous structure is a castle which unfortunately today is nothing more than ruins. You should also check out a leaning tower that is a tribute to the love of a knight for one of the local girls.
4. Wilanow Palace
This impressive palace is located on the border of Warsaw, the capital of Poland. It was constructed in the 17th century and it stands on huge manicured lawns all around it. This palace was passed down from one royal family to another before finally falling into private ownership. It was finally declared a national treasure around the 20th century. The design is primarily Baroque and is characterized by green and yellow towers. The tower itself is replete with royal paintings and other artifacts. The gold and red luxurious interiors provide a perfect backdrop for the display of portraits, sculptures, paintings, weapons, cutlery, and crockery that line the interior.
5. Royal Castle
This imposing castle is located in the middle of Warsaw's Old Town and has served generations of Polish royalty for several centuries. It has been constructed in Gothic style in the 15th century and its design is characterized by bulbous spires. It has been transformed into a museum and is home to several paintings and portraits of the royal family. The castle faces a square that houses the King Sigismund's Column which was built to honor a Polish king who transferred the Polish capital to Warsaw.


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Offres d'admission
Basic Requirements for Admission
Ontario high school students must present the OSSD and complete six 4U/M courses. All required courses will be included in the calculation of your overall admission average.
Failed and Repeated Courses
We will consider the higher grade of the 2 attempts.
Timing and Process for Admission Decisions
We will begin to issue a select number of offers in December and will continue to make offers of admission through to May. Scholarship and co-op offers will accompany offers of admission.
Consideration for early admission in December will be based on high academic standing on final 3U/M grades and registration in prerequisite 4U/M courses. A very limited number of offers to high achieving students will be extended. In February, the majority of offers of admission will be based on a minimum of 3 final or 6 interim 4U/M grades, as well as registration in prerequisite 4U/M courses. All offers will be conditional and subject to review based on subsequent grades. All other offers will be based on the best six 4U/M grades (midterm/interim and finals), including prerequisite courses. If you completed 6 or more 4U/M courses, you will be considered on the basis of your final grades. In the case of repeated courses, the higher grade will be used in the calculation of admission averages without penalty.
You can expect to receive an offer of admission to only 1 program. Your highest choice listed for Brock is considered first when more than 1 Brock program is indicated on your application. If you are not eligible for the first choice, consideration will be given to subsequent choices in the order of preference you indicated. If you apply to only 1 Brock program and do not qualify, in most cases, consideration will be given for an alternative and related program. In most cases if you apply for, but are not admitted to, a co-op program, you will automatically be considered for the regular, non co-op program.


text: hi all.......my pc keeps telling me it is missing do i need it? or is it a way of getting my money? i have had my broadband up daterd to adsl2 on aol top speed only lasted a few days. i run avast security and melawear for spam.please help dave


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Create a lookup node for [can-mustache.key keys].
new Scope(context, [parent], [meta] )
- context
A value that represents the current context. This is often an object or observable and is the first place a
keyis looked up.
- parent
The parent scope. If a
keyvalue is not found in the current scope, it will then look in the parent scope.
- meta
A configuration object that can specify special behavior of the context.
Returns a scope instance.
A can-view-scope represents a lookup context and parent contexts that can be used to lookup a key value.
If no parent scope is provided, only the scope's context will be explored for values. For example:
var data = {name: {first: "Justin"}},
scope = new Scope(data);
scope.get("name.first") //-> "Justin"
scope.get("length") //-> undefined
However, if a
parent scope is provided, key values will be
searched in the parent's context after the initial context is explored. For example:
var list = [{name: "Justin"},{name: "Brian"}],
justin = list[0];
var listScope = new Scope(list),
curScope = new Scope(justin, listScope)
curScope.get("name") //-> "Justin"
curScope.get("length") //-> 2
Use add to easily create a new scope from a parent scope like:
var list = [{name: "Justin"},{name: "Brian"}],
justin = list[0];
var curScope = new Scope(list).add(justin);
curScope.get("name") //-> "Justin"
curScope.get("length") //-> 2


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As part of the South West Newsplan meeting I was lucky enough to have a tour of Colindale.
British Library Newspaper Storage will soon move to Boston Spa There is no environmental control at Colindale and space is limited. There is already 28 miles of shelving and 69000 bound volumes.
It was interesting to see that volumes should be stored vertically as currently some of our bound volumes are horizontal in drawers. This is due to space restrictions but in the future it would be advisable to store correctly.
The bound volumes at Colindale are stored chronologically in the order they were bound, they are not stored geographically. Each volume has a unique number. This is very efficient but retrieval can be tricky as the same titles are stored on multiple floors.
National newspapers are stored by title due to heavy usage.
Researchers using the library only get the original bound volume if no microfilm is available. Original newspapers are still collected and will continue to be at Boston Spa. As publishers want income from digital newspapers only those out of copyright are being digitised.
It was interesting to witness the microfilming process… newspapers are ironed before being photographed! Retrospective filming is ongoing together with new papers. Digitisation from microfilm costs £1 per page and is searchable. However to digitise most papers have to be refilmed.
Microfilms are stored in a temperature controlled environment on mobile shelving.
The British Library also has 80-90% of all free press and different trade magazines for example, Melody Maker and Radio Times. The titles to be taken to Boston Spa are currently under review.
The original reading room dating from 1932 is beautiful and seems an apt place to research and discover. When reading is moved from microfilm to PC the papers will sadly not be browsable.


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Summer is Over!! Are you thinking, 'thank goodness!' OR, 'I'm sorry it's over so soon..........'?
Whatever kind of summer you've had, amazingly busy or amazingly relaxed, you're ready for a change. Either your seasonal position will end soon or you'll be looking to make a jump (or you need some serious spa time) and this is the time of year to do it.
Right now, it's what we like to call, the calm before the storm. Every year, in the fall months leading up to the Fort Lauderdale Boat Show and the Caribbean season, boats have crew changeover and it happens mostly in the months of October, November and December. You'll notice the number of jobs listed on our board and elsewhere aren't currently numerous.. but they will be.
So! If you're looking for a change or are new to the industry.. hang on to your hats as job listings ALWAYS pick up and will do so in the coming weeks :)
As well, Five Star Crew is almost ready to launch our Hire Crew search capabilities!!! Anyone looking to hire crew will be able to peruse our extensive database of candidates. Pricing will differ for EVERY category and for EVERY level of experience or licensing requirement! And it's 100% automated. We screen candidates for their uploads and overall profile professionalism and continuity. And, we present them to you in the easiest and most comprehensive way possible. Something new? We hope it's something revolutionary! But we'll let you decide. Stay tuned.........!


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Meltwater Entrepreneurial School of Technology (MEST) continues expansion across the African continent, officially announcing recruitment of Entrepreneurs-In-Training (EITs) from Côte d'Ivoire, the first French-speaking country in which MEST will have a presence.
The news will be publicly announced at the Africa Web Festival in Côte d'Ivoire, taking place 30th November- 1st December, in front of 6,000 attendees. Throughout the week, MEST is also hosting recruitment sessions for the next cohort of EITs.
The expansion into Côte d'Ivoire is a strategic move for MEST, marking a foothold in its fifth African country, and bridging the gap between the English-speaking and French-speaking African economies to expand the tech ecosystem even further.
With the addition of Côte d'Ivoire, MEST is now accepting applications from Ivorians, Ghanaians, Kenyans, Nigerians and South Africans who wish to spend a year at the flagship campus in Accra, Ghana to take part in an intensive entrepreneurial training program, launch their own software startup, and pitch to receive seed funding from the Meltwater Foundation.
The MEST expansion strategy remains the same: a phased rollout of incubators in key economic centers on the continent, replicating the successful operating model and expanding the power of the network for all MEST portfolio companies to quickly scale across Africa once they've found an idea that works.


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Discussion in 'Computers' started by movan, Sep 20, 2012.
:Sad: Does anybody know how to unscramble a problem on a website page which is only applicable to my computer, please? I have downloaded C Cleaner and used it .. someone mentioned something about @DNS Cache, whatever that is. Can anyone suggest something please, bearing in mind I have a computer phobia and don't understand technical terms. Thank you.
Dingray's photo above downloaded fine.
Subscribers don't see these adverts


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Servants Heart Ministry 2014 Privacy Policy
One mission... One passion... One Child!
Servants Heart Ministry is a network of Christians that exist to help the underprivileged children of the world that don't have a voice.
We met on a mission trip to the Ukraine in 2006 while building homes for orphans. Two years later, we began this ministry through faith and we are on a course to change as many lives as possible through Jesus. We all agree that the Dominican Republic, one of the poorest nations in the Northern Hemisphere, is a good place to start.
Who Are We
Latest News
Our Volunteers
Our Mission
We are so thankful to have such amazing volunteers.
Without volunteers nothing would be accomplished. This ministry is operated, on the US side, with 100% volunteers. These volunteers work and serve purely out of faith and compassion. Most of our foreign staff are volunteers as well with the exception of a few stratigic program administrators. We wish to thank each and every one of our volunteers from the bottom of our heart.
To impact and change the lives of children around the world through missions for Christ by providing life sustaining necessities, sending, supporting and maintaining mission teams, construction and the sponsoring of children in order to lead them to Christ and break the cycle of poverty.
October 3, 2013
Our fourth program will be underwaqy beginning on November 1st of this year. This will mean that we are reaching another 100 children.
Building on a vision
Our inagural trip was in June 2008 and witnessed the extreme poverty that no child should have to grow up in. With a servants heart we will establish multiple programs to help meet some of the basic needs of God's children. Join us either in person, prayer or with your financial support to help make a difference in the world.
Changing Lives...Once Child at a Time!
October 2, 2013
Groundbreaking for a church plant in Las Canoas has begun. We hope to be completed early 2014. Funding still needed to complete the church.
September 26, 2013
The CEO and COO travel to the DR to look at potential new community and meet with engineer to begin the minstry-first church plant.


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Today it's said that more than 84 million tuned in—the biggest audience since the Carter/Reagan debate—and that doesn't even count the number who watched the debate on their computers and streaming devices. Yet it's unlikely that many minds were changed by last night's debate. Voters who were looking forward to a knock-out punch by either candidate were disappointed; each candidate delivered "more of the same."
In the first of three scheduled presidential debates, Trump tried to pin the blame on Clinton for chronic problems facing the US, while Clinton put Trump on the defensive with a poised and well-prepared performance. Clinton denounced Trump as racially insensitive, accused him of "stiffing" small businesses that did work for him, and said he was resisting calls to release his tax returns because he had something to hide. Trump derided Clinton as a "typical politician" and said she had failed to affect positive change in her 30 years in public life.
Election day results will probably prove that the debates are irrelevant. Voters will likely vote their prejudgments as the main opinion polls show both candidates in a virtual tie. Post-debate results are reminiscent of both candidates' post-convention numbers.
Public Policy Polling's post-debate survey found that 51% of voters nationally said Clinton won, while 40% favored Trump. In a CNN/ORC snap poll, 62% said Clinton won, while 27% said Trump had a better night. Republican pollster Frank Luntz had a focus group of undecided voters watch the debate for CBS News, and the group named Clinton the victor 16 to 5. "This is a good night for Hillary Clinton, it is not a good night for Donald Trump," Luntz said, "but there is still time and there are still undecided voters."
"Markets started to call the debate for Hillary within the first 15 minutes or so, with the Mexican peso surging in what is probably its busiest Asian session in years," said Sean Callow, a senior currency analyst at Westpac in Sydney, Australia. For the markets, the experienced Clinton, a former secretary of state and senator, is seen as the candidate of stability and the status quo, while Trump, an opinionated narcissist billionaire, represents uncertainty.
Even if the first polls last night found that most viewers believed that Hillary Clinton won the debate against Donald Trump—who cares? Each candidate delivered pretty much as expected. Low bar, high bar—it matters not. For his part, Trump's justifications for his claims pretty much boil down to "because I told you so." Clinton's boil down to "wonk, wonk, wonkety-wonk, blah-blah-blah."
What a choice.
Groove of the Day
Listen to the Kings of Leon performing "Don't Matter"
Weather Report
70° and Partly Cloudy


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Get a free shipping quote.
Harlanssonnysimage aka Harlan is a registered AQHA 09 dun gelding. Harlan has been saddled 3 times and is still very green. Harlan is 14.3 and gorgeous will be an extremely nice horse. If you have any questions or if you would like to come see him please contact us.
The 12 Star ranch is a horse, cattle, goat, and hay operation. We raise quality registered APHA and AQHA horses. We also raise kiko goats and run a large cattle operation. If you would like more information visit our website or give us a call.
Avoid Scams and Fraud - Potential areas of fraud: wire transfer, moneygrams, money orders, cashier checks, shipping, escrow, "transaction protection", "guarantee". Check out the Buyer Safety section for more info.


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Today was such a beautiful day! (Sorry, I didn't bring my camera out with me for photographic evidence.) The sky was so clear and blue and the clouds were big and white and fluffy. And the sky was so clear! (Did I already mention that one?) I could see the mountains in the distance! Everything looked so clear!
...And that's when you remember you've lived in the DF for a while now and this is noteworthy because 1. it's currently the rainy season and 2. even on non-rainy days the city isn't exactly known for it's great air quality.
It was a beautiful day though :)


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I like shoes allots ... LOTS ! I like boots more especially the ones that reach my knees 😯And I'm done with boys .... Men though ;) I'm not wearing knee high boots for nothing ;p
1. 👠👠 👠👠 👡👡 👠👠👟👟👢👢👠👠👡👡👟👟👠👠👢👢
2. Men
In that order.
me? A lot.
Not often. I'm perfectly fine with my looks.


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Cute Backgrounds for PowerpointAugust 24, 2008 # 5:33 am # Computer and Notebook, Fun Off-Topic # 11 Comments
Girls usually search for free cute backgrounds to make presentation or just to create the good powerpoint presentation. Now you don't need to worry where to search that cute background or any cute wallpaper to make a really cool presentation. My team made it a few months ago and place it on our sister site. In that site, you can download free and ready to use ppt and pot powerpoint templates.
This themes is inspired from Katamari Damacy, my favourite PS2 game. The cute background images in those presentation is Ichigo, the cousin of prince in the game. This cute themes can be downloaded for free here. Don't worry, we never charged you In powerpoint menu, you also can save as the background if you want to make it turn it into free desktop background. And voila, you will get a really nice background for your PC!
PS: You may also interested with my previous works of Cute Powerpoint Templates here


text: Home > Sports STARmen buck PRC 5, rule MBL The Philippine Star Posted at Jan 24 2013 02:49 PM | Updated as of Jan 24 2013 10:49 PM Share Save Facebook Twitter GPlus LinkedIn MANILA, Philippines - Niño Marquez took charge down the stretch, hitting six charities in the last 90 seconds as STAR Group survived a rallying PRC Couriers side, 87-84, to retain the MBL Corporate Cup 2 basketball crown at the San Andres gym in Manila Tuesday night. The STARmen blew several double-digit margins but went to Marquez in the closing minute to preserve their lead and complete a nine-game sweep of the tournament sponsored by Ironcon Builders, Cebuana Lhuillier, Zucchero Cafe and Sports Bar-Intramuros, Jollibee Luckygold-Pasig, Dickies Underwear and KABAKA of Cong. Amado Bagatsing. Marquez went on to finish with 32 points and bag the MVP honors in the event ruled by STAR Group, handled by coach Alfred Bartolome and manager Mike Maneze, for the second straight year. PRC actually forced a tie at 81 on a three-pointer by Edwin Asoro with still 3:30 left but Marquez forced the issue inside, drawing fouls from PRC defenders while canning in the crucial free throws in the clutch. PRC still had two chances to send the game into overtime, but Norman Goco and Mar Reyes each misfired from the long range as time expired. MBL chairman Alex Wang awarded the trophy to the STAR Group assisted by commissioner Boy Reglo. Aside from the trophy, Marquez also took home an MBL Chairman's Watch and Jollibee Luckygold-Pasig gift certificate. The Manila Capitals of Mayor Alfredo Lim and MASCO chairman Paul Edward Almario finished third. Ver Roque and Gio Coquilla added 12 points apiece and Mario Geocada made nine points for The STAR, which also defeated PRC twice in the elimination round. Anthony Cuevas led PRC with 20 points, including nine at the height of his team's furious rally in the fourth quarter. Mel Mabigat added 15 and Reyes had 11 points. Share Save Facebook Share on Twitter GPlus LinkedIn Read More: MBL Corporate Cup 2 /entertainment/11/20/17/bea-alonzo-throws-party-for-moms-50th-birthday/video/entertainment/11/20/17/golden-disc-idaraos-sa-pilipinas/entertainment/11/20/17/maricar-reyes-to-write-tell-all-book/news/11/20/17/panoorin-gaano-kahirap-mag-mrt/sports/11/20/17/dennison-hopes-for-another-uaap-finals-stint-before-leaving-feu


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For in vivo applications of magnetically labeled stem cells, biological effects of the labeling procedure have to be precluded. This study evaluates the effect of different Ferucarbotran cell labeling protocols on chondrogenic differentiation of human mesenchymal stem cells (hMSC) as well as their implications for MR imaging.
hMSC were labeled with Ferucarbotran using various protocols: Cells were labeled with 100μg Fe/ml for 4h and 18h and additional samples were cultured for 6 or 12 days after the 18-hour labeling. Supplementary samples were labeled by transfection with protamine sulfate. Iron uptake was quantified by ICP-spectrometry and labeled cells were investigated by transmission electron microscopy and by immunostaining for ferucarbotran. The differentiation potential of labeled cells was compared to unlabeled controls by staining with alcian blue and hematoxylin & eosin, then quantified by measurements of glucosaminoglycans (GAG). Contrast agent effect at 3T was investigated on day 1 and day 14 of chondrogenic differentiation by measuring signal-to-noise ratios on T2-SE and T2*-GE-sequences.
Iron uptake was significant for all labeling protocols (p< 0.05). The uptake was highest after transfection with protamine sulfate (25.65 ± 3.96 pg/cell) and lowest at an incubation time of 4h without transfection (3.21 ± 0.21 pg/cell). While chondrogenic differentiation was decreased using all labeling protocols, the decrease in GAG synthesis was not significant after labeling for 4h without transfection. After labeling by simple incubation, chondrogenesis was found to be dose-dependent. MR imaging showed markedly lower SNR values of all labeled cells compared to the unlabeled controls. This contrast agent effect persisted for 14 days and the duration of differentiation.
Magnetic labeling of hMSC with ferucarbotran inhibits chondrogenesis in a dose-dependent manner when using simple incubation techniques. When decreasing the incubation time to 4h, inhibition of chondrogenesis was not significant.
Matrix-associated stem cell implantation (MASI) therapies have been developed to provide regeneration of bone and cartilage defects of arthritic joints. Human mesenchymal stem cells (hMSCs) are a promising and realistic tool for cell-based therapies, because they are easily obtained by a bone marrow aspirate, well characterized and efficiently expanded in vitro.
The plasticity and capacity for self-renewal generate the unique potential of hMSCs for synovial joint regeneration. hMSCs can differentiate into all components of a synovial joint, such as bone, cartilage, tendons, fat, and muscle [1, 2]. In recent years, various techniques and protocols for hMSC differentiation into bone and cartilage have been developed. Such techniques include dedicated growth factors [3] or culture substrates [4] to trigger directed differentiation in 3D matrixes [5–7].
hMSCs can be labeled with iron oxide nanoparticles and can be visualized non-invasively in vivo with MR imaging over several days [8, 9]. Direct non-invasive in vivo visualization of hMSC transplants in cartilagenous defects would improve spatio-temporal monitoring and allow a non-invasive confirmation of stem cell homing and engraftment or an early diagnosis of graft failure due to cell death or migration from the transplantation site.
The potential clinical application of this imaging technique require that labels, such as iron oxide, do not impair the viability or function of the transplanted stem cells. Recently, Bulte et al. reported impaired chondrogenesis of hMSCs after labeling with 50μg Fe/ml Ferumoxides-polylysine for 24 hours [10]. This may have been dose-dependent, since the same authors demonstrated a smaller degree of impairment after incubation with half of the initial contrast agent concentration [11]. Finally, the same authors showed that Protamine sulfate by itself does not inhibit chondrogenic differentiation [11].
An alternative iron oxide contrast agent is Ferucarbotran (Resovist), which is approved for clinical applications in Europe. In comparison to Ferumoxides, it has a higher labeling efficiency for non-phagocytic cells [12] and a higher threshold for toxic effects than Ferumoxides [13] i.e. impaired viability and/or apoptosis of labeled cells was noted at higher iron concentrations of Ferucarbotran when compared to ferumoxides. It has also been shown that hMSC can be efficiently labeled with Ferucarbotran without the need of a transfection agent [14, 15].
Thus, the purpose of this study was to evaluate the effect of Ferucarbotran labeling on the differentiation potential of hMSCs into chondrocytes. We compared the effect of different iron concentrations and labeling techniques on hMSC differentiation in order to determine the optimal protocol that would provide the least impairment of the differentiation process.
The MR contrast agent Ferucarbotran (Resovist, Schering AG, Berlin, Germany) was used for this study. Ferucarbotran is a superparamagnetic iron oxide (SPIO) particle with a crystalline nonstoichiometric Fe2+ and Fe3+ iron oxide core. A carboxydextran coat with a mean hydrodynamic diameter of 62 nm stabilizes the particle, ensures aqueous solubility and results in a net negative charge for the particle [16–18]. The r1 and r2 relaxivities are 7.2 ± 0.1 mM−1s−1 and 82.0 ± 6.2 mM−1s−1 respectively (in blood at 37°C and 1.5 T) [18]. Ferucarbotran was approved for clinical MR imaging of the liver in Europe in 2001 [17]. Ferucarbotran can also be used for ex vivo cell labeling and subsequent in vivo tracking of labeled cells. The iron oxide particles are internalized into cells by endocytosis and then stored in secondary lysosomes within the cytoplasm [13].
Human mesenchymal stem cells (hMSC, Lonza Walkersville, Inc., Walkersville, MD, USA), were cultured in Dulbecco's Modified Eagle Medium (DMEM) High Glucose media (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Hyclone, Logan, UT, USA) and 1% Penicillin-Streptomycin in a humidified 5% CO2 atmosphere at 37°C. Cells were passaged upon 90% confluency and either used for experiments or redistributed to new culture flasks. All experiments were performed in between passages 6–10 to avoid senescence and ensure full chondrogenic potential.
The cells were labeled with Ferucarbotran (Resovist®, stock concentration: 500 mmol Fe/ml, Bayer Schering AG, Berlin, Germany) using the following incubation protocols:
Cells were labeled in T 175 cm2 tissue culture flasks (Nalge Nunc, Rochester, NY, USA) with 20 ml of labeling media. All labeling solutions were prepared with serum-free media. For all protocols using simple incubation, FBS was added to a final concentration of 10% after 2h of initial incubation with labeling media. For protocol (3) using protamine sulfate an equal amount of media containg 20% FBS was added after 2 hours of incubation. FBS was added in order to maintain the standardized culture environment and thereby to prevent undirected differentiation. After the cell labeling procedures, the cells were trypsinized and washed three times with PBS (pH 7.4) by sedimentation (25°C, 400 g, 5min) and resuspended in complete DMEM growth media. Cellular viability of labeled cells and unlabeled controls was assessed by the trypan blue exclusion assay (Sigma Aldrich, St. Louis, MO). The concentration of iron within the labeled hMSCs was determined by inductively coupled plasma atomic emission spectrometry (ICP-AES, IRIS Advantage, Thermo Jarrell-Ash, MA, USA). For this, triplicate samples of 5 × 105 unlabeled and labeled cells were collected in 400μl of media containing no supplements. Intracellular iron uptake is known to be inhomogenous for different cell types and different labeling protocols [21, 22] and therefore results are presented as mean intracellular iron content.
Chondrogenic differentiation of triplicate samples of Ferucarbotran labeled hMSCs (as explained above) and of unlabeled controls was induced following a standardized protocol [19, 23] (Lonza, Walkersville, MD, USA). In brief, aliquots of 2.5*105 hMSCs were resuspended in 0.5ml of complete chondrogenic media (Lonza) containing 10ng/ml rTGF-s3 (both Lonza). The cell suspension was transferred into polypropylene centrifuge tubes (VWR, West Chester, PA, USA) and centrifuged at 150 g for 5 minutes. Pellets were then incubated at 37°C and 5% CO2 for 14 days. The media was changed every 2 days and chondrogenic pellets were harvested after 14 days in culture.
Quantification for accumulated sulfated glycosaminoglycan (s-GAG) was performed as previously described [24, 25]. In brief, each pellet was digested at 65°C overnight with 1 ml of papain solution (25g/mL in 50 mM of sodium phosphate, 2 mM of N-acetyl cysteine, 2 mM of EDTA, pH 6.5). The dimethylmethylene blue (DMMB) assay was used to determine the s-GAG content of papain digests, using chondroitin sulfate C (sodium salt from shark cartilage, Sigma, St. Louis, Missouri) as a standard. A microplate reader was used for spectrophotometric measurements (Model 3550, BioRad; Hercules, California, USA).
The chondrogenic pellets underwent MR imaging at 3T on days 1 and 14 of chondrogenic differentiation on a clinical MR scanner (Signa EXCITE HD 3T, GE Medical Systems, Milwaukee, WI, USA) using a standard circularly polarized quadrature knee coil (Clinical MR Solutions, Brookfield, WI, USA). The tubes containing the pellets were immersed in a water bath to avoid susceptibility artifacts from surrounding air and then scanned at room temperature (20°C). MR images were obtained using axial and coronal spin echo (SE) sequences with a fixed TR of 2000 ms and multiple TE (60, 45, 30, 15 ms) values. In addition, T2*-weighted axial and coronal gradient echo (GE) images were obtained with a flip angle of 30 degree, a fixed TR of 500 ms and varying TE values (28.8, 14.4, 7.2, 3.7 ms). All sequences were acquired with a field of view (FOV) of 160x160 mm, a matrix of 256x196 pixels, a slice thickness of 5 mm and one acquisition.
MR images were transferred as DICOM images to a SUN/SPARC workstation (Sun Microsystems, Mountain View, CA, USA) and analyzed using a dicom-dedicated image processing software (Osirix, UCLA, Los Angeles, CA). Signal intensities (SI) of the different pellets and SI of background noise were measured on coronal MR images, using operator-defined regions of interest (ROI). Average size of ROIs measured 6mm2. For measurements of background noise, ROIs were placed in the air surrounding the waterbath. The MR signal of each pellet was quantified as signal-to-noise-ratio (SNR): SNR = SI (pellet/noise [26]. Care was taken to analyze only data points with signal intensities significantly above the noise level. SNR was compared in between labeled cell pellets and unlabeled controls.
Control and labeled cells were plated on Thermanox coverslips and let adhere as adherent cell cultures overnight. They were then fixed directly on the slides with 2% glutaraldehyde in 0.1M sodium cacodylate buffer, postfixed with 1% osmium tetroxide followed by 2% aqueous uranyl acetate. The samples were dehydrated with ethanol and embedded in epoxy resin. Ultrathin sections of 80 nm were stained with 2% uranyl acetate and Reynolds lead-citrate. They were examined and photographed at 80kV in a JEOL 100CX II (JEOL, Tokyo, Japan).
Control and labeled cells were plated on multichamber glass slides (Nunc, Rochester, NY, USA) and cultured as described above. Once attached, cells were fixed at room temperature with Carnoy's solution and washed three times with PBS. Samples were then incubated with an anti-dextran FITC antibody (Stem Cell Technologies, Tukwila, WA, USA) at room temperature for 60 minutes to stain for dextran-coated iron oxides. Finally, slides were washed three times with PBS and counterstained with DAPI (Vectashield with DAPI, Vector Laboratories, Burlingame, CA, USA). Samples were analyzed by confocal microscopy at 40x (Zeiss LSM 510, Thornwood, NY, USA).
Chondrogenic pellets were stained with hematoxylin & eosin (H&E) for structural analysis. They were also stained with alcian blue for glucosaminoglycans to evaluate cartilage matrix deposition. The pellets were washed in PBS, fixed in 10% Neutral Buffered Formalin and encapsulated in HistoGel (both Richard-Allan Scientific, Kalamazoo, MI, USA). After paraffin embedding, sections of 5μm thickness were cut and stained with H&E and alcian blue. H&E sections were evaluated for the presence, distribution and morphology of cells. Each section was scored to assess the fraction of spindled cells as follows: 0 = 0 %, 1 = 1 – 33 %, 2 = 34 – 66 %, 3 = 67 – 100%. The intensity of alcian blue staining was used to quantitate the abundance of cartilage matrix, as follows: + = weak stain, ++ = moderate stain, +++ = most intense stain.
Statistical calculations were performed using the statistical software SPSS (SPSS Inc. Chicago, Illinois, USA). Measurements of cell viability, of cellular iron uptake and of GAG content in cell pellets were acquired in triplicates. Mean values and standard deviations were calculated. Labeled samples were compared with unlabeled controls for significant differences with a t-test on a 5%-level of significance. Repeated pairwise comparisons of one sample were adjusted with the Bonferroni correction.
Trypan blue staining showed no significant decrease of cell viability of labelede cells (Table 1). Spectrometry measurements showed that all incubation protocols resulted in a significant mean cellular uptake of iron oxides compared to unlabeled controls (Figure 1, Table 1). The uptake of Ferucarbotran into hMSCs was significantly higher after simple incubation with Ferucarbotran for 18 h compared to 4h (p = 0.0002). Cellular uptake of Ferucarbotran was significantly increased after transfection with protamine sulfate, regardless of incubation time (p < 0.05). The iron content of the hMSCs labeled with the standard protocol was not significantly decreased at 6 days after labeling, but was significantly decreased at 12 days after labeling (p = 0.15 vs p = 0.0001). The iron uptake could be macroscopically appreciated as brown staining of the chondrogenic cell pellets (Figure 1).
All Ferucarbotran labeled chondrogenic cell pellets showed reduced GAG synthesis compared to unlabeled controls. This difference was significant for all labeled pellets except for the 100μg 4h sample. All chondrogenic cell samples showed an inverse relation between GAG production and iron uptake, except for the sample labeled with ferucarbotran and protamine sulfate (Figure 1). hMSCs labeled with Ferucarbotran for only 4 hours showed a significantly higher GAG production compared to hMSCs that had been labeled with Ferucarbotran for 18 h. In addition, hMSCs that had been cultured for 6 or 12 days in between labeling and induction of chondrogenesis showed a higher level of GAG synthesis compared to labeled hMSCs, which had undergone chondrogenic differentiation directly after 18 h labeling.
MR images of chondrogenic pellets showed a very similar contrast agent effect on day 1 and on day 14 of chondrogenic differentiation. There was marked signal loss of labeled hMSCs compared to the unlabeled controls on T2 and T2* images (Figure 2). This area of signal loss exceeded the size of the labeled cell pellets. Corresponding SNR values were at least 10-fold lower for all labeled cell pellets compared to the unlabeled controls (Figure 2). SNR data of labeled pellets (representing the magnitude of signal loss) were not different for the applied T2 and T2* sequences. However, the susceptibility effects of labeled pellets (i.e. area of signal loss) were larger on T2* compared to T2-images. It was not possible to evaluate either T2 or T2* relaxation times for chondrogenic cell pellets because the MRI signal of the cell pellets was at the noise level using the minimal TE setting of our clinical scanner. Therefore, we compared the signal-to-noise ratios based on T2 and T*2 sequences.
Confocal microscopy studies of labeled hMSCs, stained with anti-dextran FITC antibody showed contrast agent associated with the cells (Figure 3). It was found that fluorescent staining varied in intensity in between cells labeled with the same protocol. By comparison, the unlabeled control cells did not show any fluorescence. Electron microscopy studies confirmed the presence of iron oxide nanoparticles in secondary lysosomes of labeled cells, but not in unlabeled cells. Electron microscopy also showed clusters of iron oxide particles next to the cell surface for our standard protocol but no extracellular iron for all other protocols. hMSCs labeled by simple incubation for 18 h showed a higher number of iron oxide particle-containing lysosomes compared to hMSCs labeled for 4h. Similarly, hMSCs directly after 18h Ferucarbotran labeling showed more iron oxide-containing lysosomes than hMSCs, which had been cultured for 6 or 12 days after labeling. hMSCs labeled with the transfection agent Protamine sulfate showed fewer but larger iron oxide-filled lysosomes compared to hMSCs that had been labeled without transfection agent. The intensity of FITC staining on confocal microscopy and the amount of intracellular iron on electron microscopy images correlated well with the iron uptake measurements as acquired by spectrometry.
After chondrogenic differentiation for 14 days, the pellets showed different degrees of chondrogenesis. Representative samples are shown in Figure 3 and a semiquantitative analysis for all samples can be seen in Table 2. H&E stains of control samples confirmed differentiation from primitive, round precursor cells with a high nuclear to cytoplasmatic ratio to spindle cells with a lower nuclear to cytoplasmatic ratio (Figure 3). The samples labeled with 100μg Fe for 4h showed a similar morphology compared to the control samples, while samples labeled with 100μg Fe for 18h or cells that were incubated for 6 or 12 days after 18h labeling showed little differentiation. Cells that were labeled with 50μg Ferucarbotran and Protamine sulfate showed an even lesser degree of differentiation, retaining more the primitive, round-cell phenotype.
Alcian blue staining for glucosaminoglycans was most intense for the control sample (Figure 3). A moderate staining was found for hMSCs that had been incubated with 100μg Fe for 4h and after an additional incubation of 12 days. Weak staining was found after incubation for 18h, after an additional incubation for 6 days and after transfection with protamine (Table 2). In the sample incubated with protamine sulfate, the positive staining by alcian blue is partly obscured by the iron deposition.
The presented data demonstrate a dose-dependent inhibition of chondrogenesis of hMSCs that have been labeled with the SPIO Ferucarbotran. Thus, we confirm results of other groups that described inhibition of chondrogenesis by magnetic labeling with the SPIO Ferumoxides [11]. With our study, we evaluated several variables that may cause the previously described inhibition of chondrogenesis: intracellular iron quantity and presence or absence of extracellular membrane-bound iron. The systematic analysis of spectrometric, histopathologic and ultrastructural data on cellular Ferucarbotran uptake and metabolism led to the development of an improved labeling protocol (100μg Fe/ml for 4h), which does not significantly interfere with chondrogenesis. All labeling protocols led to a strong contrast agent effect on T2 and T2* images that did not decrease after 14 days of chondrogenic differentiation. The reagents for the proposed labeling procedures are in principle readily clinically applicable in Europe.
Adult hMSCs have been successfully used in vivo in preclinical models for cartilage engineering [27, 28]. In a human clinical trial, Centeno et al. have recently reported an increased cartilage volume after transplantation of autologous mesenchymal stem cells [29]. For such applications, noninvasive imaging of the implanted cells would be highly desirable. Experimental studies in animal models showed that direct localization of the engrafted cells allows for early diagnosis of graft failure or cell migration. For example Jing et al. labeled hMSC with Ferumoxides and Protamine sulfate and were able to track such magnetically labeled hMSC by MR imaging over 12 weeks [30]. They discovered by MR imaging that labeled cells did not home to the cartilage defect but migrated to the subchondral bone and the synovium. Thus, they proved the ability to detect graft failure by MR imaging of labeled cells. Interestingly, this finding on MR images was also confirmed for unlabeled cells by histology. Thus, a possibly adverse effect of magnetic labeling can't be the reason for failure to engraft at the site of the cartilage defect. Jing et al suggest a trapping of the injected cells in the exogenous artificial carrier matrix as a reason for the failure to engraft properly. However, intracellular iron content was not measured and can't be eliminated as an additional cause.
Maintained viability of iron oxide-labeled hMSCs is a mandatory prerequisite for any application of stem cells for cell tracking studies. Iron has a well known fundamental role in many cellular metabolic processes, including electron transport, deoxyribonucleotide synthesis, oxygen transport and essential redox reactions involving hemoproteins [31, 32]. The incorporation of iron oxides into the normal iron metabolism makes them appealing as contrast agents for cell labeling [33]. But previous studies also showed that iron oxides can impair the viability of stem cells when they are internalized in too high quantities [13, 34]. However, if applied in limited concentrations, iron oxides are slowly incorporated into the regular iron metabolism and do not change the physiology of the cells [8, 10, 11, 19, 20, 34–39]. None of the labeling procotols proposed in this study showed any impairment of cell viability of labeled cells.
Another prerequisite for successful in vivo studies is an unimpaired differentiation capacity of labeled stem cells. Other groups reported normal differentiation of embryonic or neuronal stem cells into neurons after labeling with iron oxides [37, 40]. In addition, normal differentiation of hMSCs into chondrocytes [19, 20, 41] and osteocytes [8, 20, 41] was noted after labeling with Ferumoxides [8, 19, 20] or FITC-labeled iron oxide nanoparticles [41]. However another group of investigators reported impaired chondrogenesis of hMSCs after labeling with 50 μg Fe/ml Ferumoxides-polylysine for a prolonged incubation time of 24 hours [10, 11]. This may have been related to the high quantity of internalized contrast agent into the cells, since the same authors found limited chondrogenesis of their cells after incubation with half of the initial contrast agent concentration [11]. Our results – although conducted with a different contrast agent partly confirm these results: we found impaired differentiation of hMSC with an excessive iron load and unimpaired differentiation of hMSCs into chondrocytes, when the cells were labeled with limited doses of iron oxides.
We used the negatively charged iron oxide Ferucarbotran because it is spontaneously endocytosed by hMSC and does not require the use of additional transfection agents [42, 43]. However, since Frank et al. reported no effect on the rate of differentiation after the use of the transfection agent Protamine sulfate, we decided to use Ferucarbotran in combination with Protamine sulfate in order to create a comparable experimental setup [19, 20]. Similar to studies with Ferumoxides [19], our data shows that the cellular uptake of Ferucarbotran can be significantly increased by the addition of Protamine sulfate.
The analysis of cell morphology of mesenchymal stem cells undergoing chondrogenic differentiation led to the findings that the rate of spindled cells increased during the observed 14 days of chondrogenic differentiation. This is a paradox, because undifferentiated mesenchymal stem cells in vitro are spindle-shaped and chondrocytes in vivo are round. A metaanalysis of histological images in other studies confirmed our findings [11, 19, 20, 44], however, these studies did not analyse cell morphology but staining. Right after trypsinization and at the beginning of chondrogenic differentiation, mesenchymal stem cells are round with a high nuclear to cytoplasmatic ratio. This is not the appearance of mature resting chondrocytes, which typically have ample cytoplasm and a small, central nucleus. The difference becomes more evident when looking at undifferentiated cells (Figure 3). During the course of differentiation, we saw an increase in the spindle cell component. We conclude that hMSCs are maturing from a completely round, primitive phenotype, to a more differentiated but still mesenchymal phenotype (with increased cytoplasm but spindled in shape). The cells form a matrix, which is a sign of an intermediate stage of chondrocyte differentiation. However, the cells do not reach the morphology of mature resting chondrocytes with low nuclear to cytoplasmic ratio. It is known, that in the growth plate, the proliferation zone has cells that get compressed and flattened in multicellular clusters [45]. Overall, we conclude that our somewhat paradox finding that is, differentiating chondrocytes appear spindled might be due to the growth in a pellet.
For all labeling protocols using simple incubation, there was an inversely proportional relation between intracellular iron oxide concentration and the rate of chondrogenic differentiation. In fact, the protocol yielding the lowest uptake or iron (100μg Fe/4 hours) showed no significant difference in GAG-synthesis towards the unlabeled control. This supports the theory of a dose-dependent inhibition of chondrogenesis, similar to what Bulte et al. suspected for Ferumoxides-polylysine complexes [11]. However, it has to be considered that iron uptake is inhomogenous and that the amount of internalized iron per cell varies to a certain degree [21, 22]. Therefore, even when using the same labeling protocol, the rate of differentiation might vary for each individual cell and results have to be considered valid for a certain population and not for each individual cell.
Our data showed surface-bound iron oxide particles immediately after cell labeling by simple incubation with our standard protocol, whereas there was no surface-bound iron for all other protocol variations. This finding explains why freshly labeled cells with the standard protocol tend to form clumps and are more difficult to resuspend after centrifugation when compared to unlabeled controls. It is known that chondrogenic differentiation depends highly on surface-linked cellular interactions and needs to be conducted in a 3D culture. It seems likely that surface-bound iron oxide particles could interfere with the essential mechanisms or structures.
Transfection with protamine sulfate yielded the highest iron uptake, about 3 times higher than for our standard protocol. Surprisingly, the total amount of GAG synthesized during differentiation was significantly higher than for the standard protocol (Figure 1). This contradicts a dose-dependency as the only mechanism of the inhibition of hondrogenesis. We offer two possible explanations for this: (1) After labeling with Protamine sulfate, there were no surface bound Ferucarbotran particles that could inhibit surface-linked cell-cell interactions because the transfection agent shuttles the iron oxides into the intracellular compartment more efficiently. (2) Iron oxide filled lysosomes are larger in Protamine sulfate labeled cells than for the other protocols suggesting a different mechanism of uptake or intracellular compartmentalization, which in turn might cause less interaction with differentiation-linked intracellular structures or substrates.
We recognize several limitations to this study. This study was performed on a well-characterized, commercially available cell line of hMSCs, but theoretically, each cell line can behave differently and results might vary for other primary cultures. Although we were able to quantify adverse effects of magnetic labeling on stem cell physiology, the underlying biological mechanisms remain unclear. We confirm similar findings of other groups [10, 20, 30, 46, 47] and show that the effects of magnetic labeling will have to be investigated in more detail; this will have to be studied with regards to molecular biology, biochemistry, cell physiology and cell morphology before labeling techniques can be integrated into clinical practice. Furthermore, long-term in vivo studies will have to address the biocompatibility of labeling techniques and the feasibility of this approach for clinical applications.
In summary, this study presents practical and efficient labeling techniques for hMSC using the MR contrast agent Ferucarbotran. In an in vitro differentiation model, the presented labeling methods showed different degrees of inhibition of chondrogenic differentiation. By reducing the incubation time and thereby the iron uptake, a higher rate of differentiation was achieved that did not significantly differ from the unlabeled control. These findings emphasize the need for further studies of the biocompatibility of stem cell labeling with iron oxides and other contrast agents before in vivo trials can take place.
This work was in part supported by German Research Foundation (DFG) stipend HE4578/1-2, and by the National Institute of Arthritis and Musculoskeletal and Skin Diseases; Grant Number: NIH R01AR054458
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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¡Bienvenido a pancomido!
My name is Susan Leahy and I'm an educator based in Ireland, where I've been the Head of Spanish in the King's Hospital School for over ten years. I'm curious about new teaching methods and like to experiment with them in class. Some I keep, some I ditch, some I revisit years later! I think that digital learning is important, but it has to be tackled the right way. This year has marked a big focus for me on mindfulness and well-being in my personal and professional life.
In 2015, I finished a four year term as President of the Association of Teachers of Spanish (ATS) in Ireland and am currently the Vice-President. During my four years as President, I transformed the Association, creating an online community of over 1000 educators via Facebook, bringing native teachers from Spain to deliver quality grammar workshops to our members, and creating a tradition of peer-to-peer training with the first Languages TeachMeet in Ireland taking place in 2014 along with the innovative Methodology Carousel during which our members engage in structured conversations around language teaching techniques that work in the classroom.
As well as my deep interest in continuing teacher education, I've worked with a number of universities in Initial Teacher Education. I regularly examine the Leaving Certificate Spanish Oral Examination and have corrected the Irish State Examinations at both Junior and Leaving Certificate Level. I was a member of the National Working Group on Modern Languages, which explores creative teaching & learning projects and designs programmes of Continuing Professional Development for teachers of Modern Languages in Ireland.
My students have won the ATS Debating Competition in 2016, 2013, 2011, 2008 & 2007. In 2015, one of my students achieved the second highest mark in the Leaving Certificate Spanish Examination. In 2013 and in 2009, students of mine achieved the highest mark in the Leaving Certificate Examination in Ireland. In 2012, one of my students came second in the Junior Certificate Examination in Ireland. In 2012, my then Form 1 and my then Form 5 students and I opened our classroom door to the National Centre for Technology in Education to show what we were doing. In 2011, my Form 2 students and I were filmed for the VITALL (Videoing Ideas for Teaching and Learning Languages) initiative to showcase examples of good practice in language teaching.
I'm always interested in hearing from other teachers and from students of Spanish, so if you have any ideas, suggestions or questions for and about the blog, please let me know.
To get in touch, send a message to khspanish[at]gmail[dot]com. If you're an educator, connect with me here on Twitter. If you're a student, follow my Facebook page or connect with me here on Twitter.


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The Lakeshore Technical College Student Chapter of Society for Human Resource Management (SHRM) is seeking donations of gently used professional attire for the 4th annual Career Closet event. Men's and women's professional attire, all sizes, can be dropped off at the Manitowoc and Sheboygan Job Centers, or at the Career Placement Center of the LTC Cleveland campus from April 1st through April 15th.
LTC's Career Closet opened in spring 2011 through the vision and leadership of two dislocated workers in collaboration with the Career Placement Center. These two students, along with many of their peers, faced career changes and financial challenges that impacted their confidence as well as their wardrobe. The Closet is a key resource to help all students across the college. The Closet offers free access to donated professional attire for men and women, including suits, collared shirts, shoes, ties, belts, jewelry, purses, and padfolios. The Career Placement Center and Closet volunteers also offer advice on the job search, resume reviews, and clothing ensembles to build confidence and create a successful first impression. The Closet has served over 250 students since 2011.
"A lot of our students are challenged economically to create the professional appearance that gives them the confidence boost they need to project well to an employer," said Doug Hamm, LTC faculty advisor of SHRM. "Last year was our biggest event ever with 100 students served.
The Career Closet is open to LTC students April 22-24. Interested community organizations who would like access to the Closet and for any questions about Career Closet donations, contact the LTC Career Placement Center at 920.693.1387 or email@example.com.
About Lakeshore Technical College
Lakeshore Technical College is a leading provider of technical education offering more than 120 career programs including associate degrees, technical diplomas, technical certificates and apprenticeship programs. In addition, LTC offers distance learning, customized training to business and industry, and continuing education opportunities.
Serving a 200,500-resident population in east central Wisconsin, LTC operates a main campus in Cleveland and additional learning sites at LTC Manitowoc, LTC Sheboygan, LTC-Plymouth Science & Technology Center, and the Lakeshore Culinary Institute. The college also operates Community Education Centers at area high schools in Elkhart Lake, Cedar Grove-Belgium, Howards Grove, Kiel, Mishicot, Oostburg, Plymouth, Random Lake, Reedsville, and Two Rivers; and 27 distance learning sites. LTC's mission is to enrich lives and strengthen the economy by preparing a workforce that is skilled, diverse and flexible. On average, 87% of LTC graduates work in the LTC District which includes Manitowoc and Sheboygan Counties, and parts of Calumet and Ozaukee Counties. Visit LTC at: gotoltc.edu.


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When I think back to one of the most revolutionary times in my photography journey, the first month I ever challenged myself, with the B&W October Challenge, sounds a very loud gong.
Part of the value stems from that being the beginning of the PhotoChallenge.org idea. However, I really believe that narrowing down my focus to shooting only with black and white in mind as the final shot did a number of things. First, I was forced to conceive how colors would balance in monotone. I also had to "see" how the ultimate overall contrast would balance out. There's just so much I was forced to do, and I believe that ultimately grew me into a better photographer.
With this year's upcoming, and relaunching, 2013 PhotoChallenge, I'm considering a personal challenge, on top of the regular themes, of shooting in black and white, all year. There will be times when I cannot stick with it, for personal reasons. However, I think a goal of shooting B&W for all my artistically motivated shooting will result in great growth.
And who doesn't love growth!? Unless you're talking about your midsection. 😦


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Closed: 03 April 2017
Posted by Positive Action
Job Description
Manager, 15 hours per week, c£12K (equivalent to £30K F/T) +benefits
As part of a significant service review the Board of Trustees have recently updated Positive Action's aims to reflect a more contemporary position and as such, have restructured the organisation including: staffing, services, resources, finances and organisational capacity.
In creating a completely new style Positive Action which will be more slim-line and member focussed - through education, peer-led support and technological support - we are recruiting for a new staff team to support this development.
As Manager, you will work closely with the Trustees and support the other two staff in developing the new model which aims are to support people living with HIV (PLWH) who become members of Positive Action by:
- Providing a structured peer mentoring programme
Providing 'Living with HIV' workshops to our existing members, those newly diagnosed or those who are new to our area
Providing a signposting information service (digital and telephonic)
Connecting PLWH to other PLWH for mutual support
Delivering education and raising awareness of HIV to the wider community
Enabling the voice of PLWH in Hampshire and Surrey
Based in Aldershot you will support the development and delivery of the service across the whole of Surrey and Hampshire. You will have:
An empathy for all those affected by HIV and an awareness of the impact of stigmatisation and prejudice surrounding HIV
Experience of managing a support service within the voluntary sector
Sound working knowledge of relevant legislation (charitable, employment, safeguarding)
Ideally have experience of successful income generation through fund-raising and grant applications
The ability to work flexibly to meet the needs of the organisation.
The client requests no contact from agencies or media sales.


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CHARLOTTE, NC (WWAY) — A billboard in Charlotte is causing controversy for showing Santa Claus with an assault rifle.
The sign reads quote: Santa knows what you really want. The billboard is for the retail store Blackstone Shooting Sports.
The billboard was recently put up on Interstate 77 in Charlotte. The image of the gun-toting Santa focusing on a target has many people in the area shaking their heads.
Click here to see what the comapany had to say about it from WSOC-TV.


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Feeding CATFISH the Best Way.
This is an excerpt from Catfish Rainfall packages. As promised we are going to show you the step by step guide to success catfish farming business even with no or little fishing experience. Guarantee!
The objective of feeding catfish is to provide the nutritional requirements for good health, optimum growth, maximum yield and minimum waste within reasonable cost so as to optimize profits.
Every farmer should be particular about the quality of feed fed to the fish because it is the feed that determines the:
☆ Nutrient loading ( and ultimately carrying capacity) in the pond, hence water quality within the culture system.
★ Fish growth rate.
☆ Economic viability of the enterprise. 60-70% of the variable production costs in a normal production cycle is due to feed.
★ Health status of the fish.
Well, you have many things to learn and gain when order for your own copy of Catfish Rainfall today.
Over 15 Mega ebook on Fish Farming and Management, videos on Feed Processes and Production, Bonus videos on Fingerlings Productions and more.
This package has been rebranded into two:
For online downloads for those that have access to internet so as to download the files.
Offline - with this, catfish rainfall package will be sent to your doorstep ( house) using courier services like dhl or nearest garage around you or post office. But this will attach an extra shipping fee of #1,000.
For more help on how to order call 08103243198 or send your questions to me as text messages or whatsapp through 08106649536.


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Skye Gould
With "Star Wars: The Force Awakens" in theaters December, everyone will be heading to a galaxy far, far away for their holiday shopping.
We've tried out the toys kids will want and searched for the gifts you'll be looking for.
Whether you're buying for a young padawan or a Jedi Master, here are some of the most-wanted items on any "Star Wars" fan's wishlist.


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Lord of the Rings Online Volume III, Book 1: Oath of the Rangers. Fans continue to pore over and discuss the information currently released as Turbine keeps interest high with screenshots, patch notes, and dev diaries.
The latest round of screenshots was released today, giving us five gorgeous new images to add to our gallery. This time they focus on skirmishes, which are set to get an extensive makeover with the upcoming patch. Look for changes to everything from Skirmish Mark drop rates to icon appearances, as well as the addition of the mysterious duo mode.
All in good time, however. Volume III is on the way, and until then we'll keep occupied with the current notes and screenshots. Take a look at the gallery below for the latest batch.


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Search for Host
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Nyasatimes.com is hosted by: Server Central Network
Hosting report about Nyasatimes.com. Nyasatimes.com is currently hosted at Server Central Network. The IP 18.104.22.168 links to a server in Chicago, United States. The company behind this all is HostForWeb.
Updated 1209 days ago
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Clark University forensic science
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Get the facts about Clark University forensic science, and registering for discussion sections. Which college degrees give you the best chances of finding employment after graduation? Whether you are considering an associates degree (CNA, LVN, or LPN), or a bachelor of science in nursing (RN), you will find many doors opening after graduation. Concentrating on Clark University forensic science, for example, you may gain an advantage in job recruiting on-campus. Check with the lab tech department for course registration deadlines, and forensics program requirements. Please use the form above to request admissions info for Clark University.
Medical laboratory technologists and lab technicians collect samples and perform tests to analyze body fluids, tissue, and other substances. An entry-level job for lab technologists usually requires a bachelors degree in medical technology or life sciences. Medical lab technicians (lab techs), on the other hand, need only an accredited associate's degree program in clinical laboratory science. Coursework emphasizes laboratory skills, including safety procedures and lab management.
Medical laboratory technologists and technicians can obtain a general certification or a certification in a specialty, such as cytotechnology or medical biology. For credentialing information, visit the American Society for Clinical Laboratory Science. Employment of medical lab techs is projected to grow 16% in the coming years. An increase in the aging population is expected to lead to a greater need to diagnose medical conditions, such as cancer or type II diabetes. Average salary for medical and clinical laboratory technologists and technicians was $50,550 in May 2017, according to the US Bureau of Labor Statistics. Beyond major requirements within the lab tech department, you may consider Norwalk Community College medical laboratory technology as a complementary elective to your primary course of study.
Bon Secours Health System
Acts as liaison between unit and Nursing Services to assist in maintaining effective knowledge of nursing care practices that may be utilized in the Cath..
4 hours ago Executive Director
We offer independent living, assisted living, Memory Care, skilled nursing, continuing care retirement, in-house therapy, hospice, home health, and personalized..
4 hours ago IMCU Monitor Technician Full-time, Days
Plantation General Hospital - Fort Lauderdale Area
This position requires knowledge of medical terminology and familiarity with medical technology as it relates to nursing. Plantation General Hospital..
4 hours ago Licensed Vocational Nurse (LVN)
Personal Care Hospice Services Inc.
Graduated of an accredited school of nursing with current CA LVN license. Minimum of 1 year experience; Licensed Vocational Nurse (LVN)..
9 hours ago Operating Room Travel Nurse (RN) (PACU)
Tailored Healthcare Staffing
Graduate from an accredited school of Nursing, BSN preferred. We are one of the nation's fastest-growing travel nursing agencies, for good reason.
5 hours ago REGISTERED NURSE
Loyola University Medical Center
Utilizes current nursing practices to provide patient care while focusing on the needs of the patient and exemplifying the service excellence goals of care..
4 hours ago


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Adoption Process
Adoption fee: $170 each includes microchip, first and second vaccinations, intestinal worm and flea treatment (to date) and desexing.
To fast track your enquiry, please apply online via our adoption form.
Contact Information: If you'd like to find out more about Zazzle, please contact: Ana at Pets Without Partners Animal Rescue Inc.Phone: 0466 631 164 or click here to email Ana.


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In the new millennium, it is nearly impossible for us to talk about basic concerns like food or gas prices, without examining global intersections in trade, energy, immigration, the environment, and defense. Many countries once described as "developing" nations now wield greater economic and political influence than some of the so-called "major" powers. Former debtor nations have become creditors of the United States and other developed countries that run budget deficits. Without recognizing and understanding these connections, we cannot even begin to discuss how the United States and other countries can craft and harness effective policies amid this breathtaking progress. In Seeing the Elephant, Peter Marber describes how increasing economic integration and the rise of new actors is drastically altering the geopolitical landscape, and offers insights on how the U.S. can make policy to maintain a leading role in the years to come.
The twenty-first century, Marber explains, demands a very different lens for viewing the world. In the era of globalization, America's success hinges on the success of its neighbors, too. Rising economic powerhouses—China, Russia, India, Brazil, and others—bring a diverse set of interests to the table that the U.S. cannot afford to ignore. Moreover, globalization has created thousands of non-state actors—corporations, banks, hedge funds, activists, and even terrorists—who bring their own concerns to bear. Marber underscores the importance of forging strong relationships with pivotal developing nations and America's need to reaffirm the centricity of global protocols, rules, and institutions after an unfortunate period of neglect. It's not too late. By focusing on seven key cross-border, interlinking issues—trade and finance, energy, security, immigration, health, the environment, and poverty—Marber recommends key adjustments in policies that aim to strengthen and modernize our current institutional infrastructure, including NATO, the WHO, the WTO, the World Bank, and the UN, among others. While his strategies do not guarantee that the United States will remain on top economically, they ensure that the global system America helped to create triumphs in the end, protecting against the factionalism that led to two World Wars and destroyed decades of economic progress.
In this timely book, Marber demystifies globalization and analyzes new international megatrends and interconnections. With bold suggestions on how America can reassert its historic leadership in the new global arena, Seeing the Elephant will show readers how the U.S. still remains the planet's best chance at building enduring peace and prosperity.


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GraphicRiver Business woman using her laptop 2717172
Stock Vector \ Characters \ People
Laptop \ Office \ Business \ Professional \ Compatible \ Blending \ Graphic
Stock Vector - Business woman using her laptop 2717172 by Millisenta @ GraphicRiver
Stock Vector \ Characters \ People
Young professional business woman using her laptop at an office. No transparency, normal blending mode – can be opened in Illustrator 8 and compatible with other graphic programs. Included files: ai (CS5), eps (8 version), high-resolution .jpeg (4000×4000 pixels).
Layered: No; Graphics Files Included: JPG Image, Vector EPS, AI Illustrator; Minimum Adobe CS Version: CS.
Abstract Map Illustration; Set for Women - Carnival Mask, Corset, Shoes; Construction Concept; Set School Supplies; Retro card with vector stylized rose ; Floral heart. Heart made of flowers; Blank post stamp with magnolia .
Keywords: activity, business, businesswoman, cartoon, characters, computer, female, homework, information, internet, job, laptop, learning, mail, modern, monitor, net, office, professional, secretary, sitting, smiling, studying, surfing, typing, women, working.


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A. This is not normal. It may be mammary cancer or benign, non-cancerous growths or cysts. I would recommend that you have a vet examine her. A sterile needle aspirate can be done to get a sample of the cells in the mass to get a better idea if it is cancer or not. It is often recommended to surgically remove these masses and spay her at the same time. Your vet may recommend bloodwork and a chest x-Ray first.
DISCLAIMER: The answers in Ask petMD are meant to provide entertainment and education. They should not take the place of a vet visit. Please see our Terms and Conditions.
IMPORTANT: The opinions expressed in Ask petMD content area are solely those of the User, who may or may not have medical or scientific training.
Our Ask petMD experts include veterinarians, vet techs, veterinary students, pet trainers, pet behaviorists and pet nutritionists. These opinions do not represent the opinions of petMD.
User-generated content areas are not reviewed by a petMD veterinarian or any member of the petMD editorial staff for accuracy, balance, objectivity, timeliness, or any other reason except for compliance with our Terms and Conditions.
petMD does not endorse any specific product, service, or treatment. Do not consider petMD user-generated content as medical advice.
Never delay or disregard seeking professional medical advice from your veterinarian or other qualified healthcare provider because of something you have read on petMD.
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International Monetary Fund (IMF) policies left healthcare systems in the African countries worst affected by Ebola underfunded and lacking doctors, and hampered a coordinated response to the outbreak, researchers said Monday.
Links between the IMF and the rapid spread of the disease were examined by researchers from Cambridge University's sociology department, with colleagues from Oxford University and the London School of Hygiene and Tropical Medicine.
They found IMF programs held back the development of effective health systems in Guinea, Liberia and Sierra Leone, the three countries at the epicenter of the outbreak that has killed over 7,370 people.


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David Manning / Staff
Oglethorpe County Probate Judge Beverly Nation rubs the unnamed foal of her mare, Ritz, on Monday while Ritz nurses Orphan Andy, a foal rejected by his own mother. Ritz, a 7-year-old quarter horse, gave birth to her foal and then immediately adopted Andy as her own.
- Read the story.
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how we work
Thank you for taking a moment to learn how we work.
Our motto is, "we help businesses win online."
Our approach begins with you, the client. We start by asking a few questions:
- How quickly do you want your business to grow?
- Why do customers pick your business vs others?
- Why are you unhappy with your current marketing?
- What does "winning" mean for your business?
Once we have your answers, we tailor an online marketing strategy specifically for you and your needs.
If you partner with us, you can expect tireless effort and methodical, results-driven work. Like our mascot Rob Oto, we're a team of marketing machines. But we aren't robots.
bizmktg.com provides online marketing solutions to dentists, doctors, lawyers, home service contractors, small local businesses, and national franchises. We are based near Seattle in Edmonds, WA and serve clients across the United States.
Our solutions include:
- Marketing Strategies & Execution
- Engaging Websites
- Reviewsof.us Online Reviews Solution
- Google Adwords Optimization
- Social Marketing Results
- Email Marketing Campaigns
- Simple Text Messaging
- Insightful Reporting
Peter Wilson founded bizmktg.com in 2012 to help small and medium-sized businesses win online.
In 1994, Peter co-founded DealerNet, the first company to make websites for franchised automotive dealerships. After selling DealerNet to Reynolds & Reynolds in 1995 he ran an online marketing business unit at Reynolds for several years. Peter has also directed business development and product management at Microsoft, Ally Financial and ADP.
Peter lives in Edmonds, WA with his wife and two children. He is active in his church and enjoys snowboarding, fishing, and kayaking.
Want to learn more about how we can help your business win online?
Contact us now for a free consultation.
(800) 808-0249 ext.2


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A Message From The Sheriff
Across our nation, law enforcement has experienced many changes over the years in the way it engages the ever-evolving criminal element.
Read More
Caroline Sheriff And Speed
Latest News Sheriff's Blog & latest updates
On 10/23/14, the Caroline County Sheriff's Office welcomed a new member to the agency. Deputy First Class (DFC), Vincent Allen, who was hired to fill a position at the Caroline County Circuit Court Security post.
DFC Allen possesses a high level of experience in Criminal Investigations and was a long time member with the Drug Task Force. Dfc. Allen comes to the Caroline County Sheriff's Office with experience in Court Security Operations from his previous agency.
PICTURED: Sheriff Randy Bounds and Chief Deputy Clerk of The Circuit Court - Terry Lord, swear in Dfc. Allen with the "Oath of Office"that every Sheriff and Deputy Sheriff takes.
Sheriff Randy Bounds presents Corporal Bill Bodnar with a Caroline County Government 5 Year Service Award. Congratulations Cpl. Bodnar!
Page 7 of 26
See the most recent messages from Caroline County Sheriff's Office, powered by Nixle.
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Business in Rocky Hill, CT
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text: …various Orthopedic, Burns, Spinal Cord Injury and Cleft Lip and Palate conditions at Shriners Hospitals for Children. Financial affordability may not be an issue in the delivery of care. The disorders and diseases that they treat are wide-ranging and diverse; therefore, this is not an all-inclusive list of conditions treated. My wife and I have a 9 yr. old daughter with cerebral palsy (CP). Her tight and weak muscles strain her physically; as a result, she experiences pain daily. Since her speech and language is delayed, she cannot express her feelings about the discomfort of having to wear braces. Upon.


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I was super honored when my friend Michele asked me to photograph her family. Kody, their sweet dog, is getting older and having more health issues, and she wanted to be sure they had photographs of the three of them together.
We photographed in Old Town Alexandria in one of my favorite gardens on a warm summer evening a few weeks ago.
Michelle and Brian, thank you for allowing me to capture your family in this season of life!


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Started Feb 7, 2015
Started this discussion. Last reply by Colorado Trash Wranglers Jun 28, 2009.
Added by Colorado Trash Wranglers
It is cool to see and hear of this project to rebuild a run down river sections on the Platte flows right through the city of Denver .
Aloha Mack is involved with wave structure" view article…Continue
Posted on February 7, 2015 at 5:30pm
Posted on December 28, 2014 at 5:42pm
Posted on June 4, 2014 at 8:59pm
Posted on June 4, 2014 at 8:30pm
June is officially IRSD as well as ISD Idaho is hosting the first real river WRSA river surf contest for money at the Payette river games http://payetterivergames.com/ sign up still open , Germans FUS crew will be shredding…Continue
Posted on June 4, 2014 at 8:00pm
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