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Do any of you grow Moringa Trees? Moringa trees are fast growing, incredibly nutritious, have more than 500 medicinal uses, can provide cooking oil, lubricants, fuel oil. I have seeds if you are interested.
Moringa Trees
Do any of you grow Moringa Trees? Moringa trees are fast growing, incredibly nutritious, have more than 500 medicinal uses, can provide cooking oil, lubricants, fuel oil. I have seeds if you are interested.
We know this tree as a "drumstick tree". The young see pods, or "drumsticks" are delicious in sambhar. They are availabe as a frozen vegetable in the markets here. I haven't seen any fresh drumsticks offered though, which is interesting.
Jackie, you have dmail.
I look forward to getting the seeds. Your thread has had a number of people looking at it. Must be some lurkers about. I think I'll try treating some of the moringas as an annual. Use the leaves like spinach and in salads, and dried for tea. I'll try to overwinter them inside and plant them out next spring along with more seed to see how they do here with a full growing season. I'm going to top them at 3 to 4 feet and trim the branches back to 1 foot when they reach 2 feet in length to make them more bushy and compact. Then I'll eat what I've trimmed off. Maybe others will try it this way.
The moringa plant is fast to mature and its leaves are readily harvested every 45 days, the tree quick to regrow them and continue its vigorous growth. Moringa leaves are packed full of protein, vitamins, minerals, co-enzymes, and antioxidants. Moringa leaves can be used in salads, cooked as a leafy green, used as moringa tea, or dried and pulverized for capsules. It�s best product for hair and weight loss as per my friends reference because they are trying their oil and pills for better health.
I have never heard of it and do not recognize it. I doubt it would grow here as we are not subtropical. Thanks for sharing the info.
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subset: commoncrawl


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(WRCB) - A former Tennessee death row inmate is suing McMinn County for $20 million.
Gussie Vann was sentenced to die for the rape and murder of his 8 year old daughter in Athens, in 1992.
Last year, the conviction was dismissed.
Vann now alleges McMinn County investigators denied him an attorney during lengthy questioning following the death of daughter.
Gussie Vann is currently serving time for an aggravated rape charge.
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WRCB Jobs EEO | FCC Public Files


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Kha'Zix Build Guide by TJDizzel
Not Updated For Current Season
This guide has not yet been updated for the current season. Please keep this in mind while reading. You can see the most recently updated guides on the browse guides page.
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Kha'Zix Build
Starting Items
More Damage
Ability Sequence
Not Updated For Current Season
The masteries shown here are not yet updated for the current season, the guide author needs to set up the new masteries. As such, they will be different than the masteries you see in-game.
G'Day Everyone.
This is a guide for a top lane, assassin, dueler Kha'Zix. He is a strong champion in the top lane as he can clear well and as excellent 1v1 potential. You should play Kha'Zix in a ranked situation when an assassin is needed and when you already have a tank. Hope you enjoy and learn from my guide.
Standard Masteries for an AD top. Butcher for easier farming. Blade Weaving and Spell Weaving as Kha'zix relies on his spells for max damage and as his Q his on a very short cooldown, it allows max damage.
Again Standard runes for an AD top. Armour Pen Quints interchangeable with Flat or Scaling AD.
Pros / Cons
Amazing burst
Excellent Duelist
Short Cooldowns
Easy farm
Often targeted in team fights
Short Range
Good positioning
High Skill cap
Order of Evolution
One of Kha'Zix unique attributes is the passive of his ultimate. This allows him to add an additional effect to a spell every time Kha'Zix hits level 6/11/16.
Evolve Q first for max damage and dueling. Then E for range and team fights. Then it depends, if you need clear speed get W but if you need more potency in team fights, I recommend R.
Starting with a long sword I have found to be most efficient as it builds into other core items and gives max damage at early levels.
Most of the time, rushing a Brutalizer is best but make a judgement on what you are trying to do in your lane. If your team needs you to push, rush a tiamat.
Frozen Mallet if you need more health and Maw of Malmortius against AP heavy team.


text: December 24, 2016 Announcement: 8th ABF moved to November 2017 Leave a comment On behalf of the local government of Ulsan Metropolitan City and Taehwa River Ecotourism, the Asian Bird Fair Exec Comm hereby would like to announce that the 8th Asian Bird Fair will be celebrated from November 17-21, 2017. More details and information on this exciting event will be shared very soon. Thank you. Share this:FacebookEmailPrintTwitterGooglePinterestLike this:Like Loading... Posted by asianbirdfair2010 in 8thasianbirdfair, Asian Bird Fair


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Janus Pit, Part 2
Earlier...July, 1976
About 200 feet into the Hassle, Joe pulled out a rock below water level revealing a miniscule crack, and we squirmed through into a 15 foot high fissure with solid walls and a ceiling comprised of huge, jammed boulders. To the left, the stream sank in a muddy sump.
To the right, an easy climb went up into randomly chocked blocks. Joe squeezed up between them and said he could see a large black space...then...
"You are in a large room, with a passage to the south, a passage to the west, and a wall of broken rock to the east. There is a large "Y2" on a rock in the room's center.
You are in a jumble of rock, with cracks everywhere.
You're at a low window overlooking a huge pit, which extends up out of sight. A floor is indistinctly visible over 50 feet below."
(From the "Adventure" caving game, by Will Crowther.)
May 6, 1978
Beyond the Hassle awaited the Baffle. The Baffle is a mass of huge breakdown blocks chocked together over the sump room, and it seemed exceedingly hazardous to push on. But the black space seen by Joe Vandiver was calling us.
Since the earlier visits, I had corresponded with Robert C. Handford, who, as a high school student, had been one of the two young cavers directed by Hugh Shell and Hale Bryant to have a look up a long, steep slope in Half Mile Cave, which broke out into what is now called the Cathedral Room in Blanchard Springs Caverns. Photo, Ozark National Forest.
It's every caver's dream to make a find of such magnitude and beauty, and we were no exception. It's such glory that drove us to return to the wet and rocky miseries of the Hassle water crawl and the breakdown hzards on May 6, 1978.
We were three: Alexia Cochrane, Robert Handford and I. We were part of a larger group of caver friends assembled to map and explore upstream, who were willing to wait for us while we made a "brief reconaissance".
At the bottom of the entrance pit, Robert realized that he'd been in the cave before, along with Mike Hill. (Referring to an article I wrote in the AACS AActivities, Vol. 3, No. 4, I see that Mike Hill had been first in the cave in March, 1970. He told Handford about it, and they returned together. Handford also had entered Flitterin' Pit but "didn't think twice about it." All of Janus known then was what we later called the December Passage.)
One More Fool Notion
that I'd had was that Alexia could make a free dive "probe" into the sump at the left of the fissure. With a rope belayed about her waist and with mask on, she was able to get into the sump far enough that only her ankles wer still visible to me. But the silting made the attempt futile as well as foolish.
The Odyssey: Hit or Myth?
As we coiled up the rope, I heard Robert Handford calling to us from the other end of the fissure room. "Tremendous! It's huge! You've got to see this."
We put the sump ducking gear down and climbed up into the breakdown. Where Joe Vandiver had tried to continue upward, we instead followed Handford's Baffling path under the blocks, slithering through a half-tube over a narrow crack in the floor, looking down into the fissure room we'd just left. Then, a couple of upward thrutches past truck sized blocks, and we stood in a vast gallery extending in both directions.
The floor was dry breakdown, the ceiling more than 60 feet overhead. In the "upstream" direction the passage diminished a bit in height, but there was a gaping hole in the left wall dropping an estimated 40 feet to a large room or passage. Beyond, the now dryer gallery sloped up into a gypsum flecked extension.
But, I am getting ahead of what really happened. We were supercharged with excitement.
Robert precipitously went downslope in the "downstream" direction to where the new room or passage, the "Cyclops' Cave" became even larger, perhaps 100 feet high and hard to say how wide. The stream reappeared out of the breakdown and channels unknown, then incised a canyon in the floor of the massive, ample breakdown caverns above. The cross section vaguely resembled a "T" or a mushroom.
Still dressed in our wetsuit halves, we sweltered up into three or more wide breakdown rooms, or might they be defined as one huge gallery but with varying sizes. Who knew, or who cared? It was overwhelming. Our guesses were that they were over 150 feet wide. The ceiling height varied according to the slope of the breakdown floor, 5 to 50 or more feet.
I chimneyed into the scallop walled stream canyon for a moment or two, hoping to cool down. It went on in walking height, the clear water riffling around the angular turnings between walls a few feet apart, but our time had run out.
|Brent Wilkins in Circe's Canyon. B. Stickney photo
We retraced the trail of our scoop back to our breakout point. I'd prudently marked the crack out of the Baffle with tape. It would have been easy to not to find the one crack among many.
(I see in my AACS AActivites article that we shared a can of Mexican condensed milk. La Lechera. Alexia must have brought it, for at that time, I'd never been to Mexico.)
Robert was bestowed the honor of naming the discovery. In keeping with his return to Janus Pit after an eight year absence, and the scope of the find, he chose "The Odyssey Series".
Our patient, quietly suffering friends were enthralled with our story, but after a three hour wait, were not yet interested in visiting it.
Totally buzzed, we went with them to the January Avenue to do some mapping! My notes say that Alan Emmendorfer freeclimbed some 25 feet up in the Atlas Dome, first climbed by Robert Taylor. A rope was tied off for future visits. (The area ends in high, chimneyable parallel fissures.)
Both Robert and I were pumped and barely able to contain our enthusiasm. He was back in the Dallas area, and I did a terrible thing. I led a scoop trip in which he could not participate. I hope he has forgiven me. It was cave mania to blame, and it infected all of us.
Meanwhile, Alexia returned with R.C. Schroeder on May 13 and mapped the Hassle through to the Odyssey on a night trip. (It's always night underground.) Kudos to them for this great effort! The crawl was mapped at over 300 feet in length. (But as these things go, I don't think any of us ever saw a finished map. Mea culpa, mea culpa, mea máxima culpa. We all have sinned.)
I succumbed to the Sin of Pride and planned a trip into the Odyssey with various Memphis area cavers on May 14, 1978. But complications arose, and in the end, only three of us went in. Larry Houston, R.C. and I. Our hopes were high for big cave beyond and were exceeded beyond our wildest imaginings.
More coming, when I can manage it.
- In our next episode:
- Circe's Canyon without benefit of moly
- Fast, fast, fast relief to obstruction
- Bypass surgery
- A Pantheistic revelation
- Aceing the test
- Taking off every Zig for great Justice
- All your base level are belong to us.
WANTED: photos of the Odyssey Series, and permission to use them; especially of the larger rooms and of the Pantheon. Leave a registered comment, and I'll email you back. A map??


text: CPD Webinars FAQs How do I purchase a CPD Webinar? To purchase a CPD Webinar please visit the CPD Courses section of our website. You can either search for your chosen event in the CPD Courses section.Once you have found your chosen event please select 'Buy Now' and follow the instructions online.When payment for this course has been processed by the Institute you will receive an email with registration information, joining instructions, conference call information and CPD Webinar FAQ's. ** Please remember if you are a CPA Member you must be logged in to avail of the CPA Member rate. What do I need to participate in a CPD Webinar? You will need a PC and internet connection. You can listen to the CPD Webinar direct from the speakers on your PC. If you wish to join the CPD Webinar by conference call you will also need a telephone. If you wish to ask questions you can do so by typing your questions through 'text chat'. How do I join a CPD Webinar? Once you purchase your chosen CPD Webinar you will receive an email with registration information. The first step in joining the CPD Webinar is to register. Following registration you will receive an email with confirmation of your registration, joining instructions containing a web link, conference call information and CPD Webinar FAQ's. Simply click this web link and follow the instructions to join the CPD Webinar. Please note you will not be able to join the webinar until the specified date and time, as per your confirmation email. I have joined the CPD Webinar, what do all the sections, buttons and icons mean? When you join the CPD Webinar you will see the following: Top left hand side – Presenter section – a picture of the presenter Bottom left hand side - Attendees section – this will list all attendees at the CPD Webinar. Beside the word Feedback you will see a drop down box. This tool enables you to interact directly with the presenter. Simply click on the most suitable heading to indicate to the presenter if you are fine, have a question, you want them to slow down or speed up, if your answer is yes or no or if you agree or disagree. Main window – this is where the presenter will share their slides and or notes with you. Bottom of screen – Text Chat – this is where you can type questions and send them directly to the presenter. For further information about participating in the CPD Webinar and using the various tools during the live CPD Webinar select 'Help' and 'Participant Online Help'. What time can I join the webinar? Please see the confirmation email for the date and start time of the webinar. Will I be able to listen to the presenter? You can listen to the presenter direct from your PC speakers. Alternatively if you have opted to also join the webinar by conference call you can listen via your telephone. Will I be able to ask questions? You can ask questions through the 'text chat'. Simply type your question in the 'text chat' box and click send. How will I see the slides from the presenter? The slides are shown live on-screen during the CPD Webinar. A copy of the slides is also emailed to all participants before the event. How do I know if my PC / system will allow me to access the CPD Webinar? Once you have successfully purchased and registered for the CPD Webinar a confirmation email with joining instructions is sent to you. This email also includes a web link to a 'System Test' which enables you to check if your PC meets all the requirements necessary to participate in the CPD Webinar. It is advised that you run this System Test in advance of joining the CPD Webinar. What happens if I can't attend the live webinar? All our CPD Webinars are recorded and can be purchased after the event through the CPD Courses section of our website. Course notes are also sent to you. If you have purchased the CPD Webinar and can no longer attend please review our CPD "Booking Terms & Conditions". What if some of my other team members want to join the CPD Webinar? Your team members are welcome to join the CPD Webinar. However, to join they must purchase the CPD Webinar in advance of the event. If they simply watch the CPD Webinar from your screen they will not receive any CPD hours. Are CPD Webinars CPD accredited? Live CPD Webinars: If you attend a live CPD Webinar the hours awarded to you will be structured CPD. 100% of your CPD hours can be achieved by attending live CPD Webinars. Recorded CPD Webinars: If you purchase and view a recorded CPD Webinar the hours awarded to you will be unstructured CPD.


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I've been seeing a lot of these posts with the same damn products on all of them and I really feel like we're recycling the same shit and there's nothing new to read anymore which is part of the reason I made this account, to add my own remedies and routines in hope that it could help someone else~
African black soap-
Lemme tell you right now. Ditch whatever shit you're using as a body wash RN and go cop some (RAW) African black soap. That shit is natural and is bursting with vitamin A and E. It helps with eczema too. I personally use Alaffia which also contains Shea butter and I use it everday. Love it and love my skin+it smells super fresh
Apple cider vinegar-
Add abt ¾ of a cup of ACV to your bath and let it work its magic. Its anti fungal and battles against body odor. It makes your hair shiny and is considered a skin detox bath, I personally experience softer skin. ALSO it resets your pH and fights again vaginal odor and yeast infections naturally too.
Jojoba oil-
Jojoba oil is the oil that everyone thinks coconut oil is. It is a natural moisturizer for hair and skin and it works way better than coconut oil could ever hope to do!!
Coconut oil-
Let me tell u something. In a previous post I copied a message on of my followers sent me abt coconut oil being horrible for your hair because it breaks down proteins. It got a lot of positive and negative feed back, some saying I was trying to speak for them or leave out black hair, no that is not the case. I simply copied a message and released it for public knowledge cause im here to HELP YOU NOT HURT YOU! I'm sorry if I offended anyone~ but coconut oil, regardless of hair, is not the angelic product everyones made it out to be. STOP PUTTING THAT SHIT ON YOUR SKIN IT CLOGS PORES. I use it to oil pull, its a natural way to remove the toxins from your mouth and it also whitens your teeth. You put abt a teaspoon or less of coconut oil in your mouth and swish for a minute or 2. Love that shit my teeth are so white. But other than that I wouldnt put that shit on my hair or skin, unless you're shaving your legs or sometin. Although I do put it on my pussy after I shave and idk what i'd do without it. But coconut oil is NOT for everything.
Rose water-
Sorry for the extremely long coconut oil excerpt lol this one's short. Rose water can be used as a toner/makeup setter. It controls oil and hydrates your skin. It also has antioxidant and antibacterial properties. Its also good on dry/frizzy hair
Castor oil+Vitamin E oil-
Another 2 great oils. I'm lazy and didnt want to make another 2 oil sections lol but basically castor oil can be used on eyebrows/eyelashes to thicken them. Its also a natural laxative. Vitamin E oil is a great hair/skin moisturizer and heals scars.
If you arent taking biotin everyday I want you to go to your local pharmacy and cop a bottle because beauty starts from the inside. Its a hair skin nail vitamin and boosts healthy growth of hair/nails and keeps skin healthy. I do love it.
Charcoal soap-
Instead of using a typical bar of soap, invest in a charcoal one instead. Charcoal contains a powder called Binchōtan which cleanses and moisturizes skin. For all u natural bitches out there.
Also a lil side note~ for anyone who wants to comment to mine or any other 'hoe tip' posts saying "these are life tip not hoe tips" SHUT UP! We know! But these tips were originally for tips and products to be as sexy and well maintained as they can be. Usually before somekind of sex appointment. Let the hoes have this instead of trying to shame it


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'Money Printing'
"Equities continue to be the only game in town," said Jeff Sica, who helps oversee more than $1 billion of assets as president of Sica Wealth Management in Morristown, New Jersey. "Why would you want to hold gold and see the value depreciate when you can buy equities and see your money grow?"
Lower prices are attracting some buyers and may boost ETP holdings in 2014, said Nick Moore, a London-based managing director and commodity strategist at BlackRock. He declined to provide a forecast.
Peter Sorrentino, who helps manage about $14.8 billion at Huntington Asset Advisors in Cincinnati, said 5 percent of its natural resources fund was allocated to gold and he wants to double that, citing prospects for faster inflation, limited supply and strengthening demand in Asia.
"At some juncture, all the money-printing will come home to roost, and at that point the value of currencies will drop," said Sorrentino. "Lots of metal has been migrating from west to the east."
Smaller Bars
Consumption in China, poised to overtake India as the biggest buyer this year, may increase 29 percent to 1,000 tons in 2013, the WGC estimates. Gold in Europe is being refined from larger bars suitable for local users into smaller sizes favored in Asia, while Deutsche Bank AG and UBS AG are among banks that opened vaults to store metal in the region this year.
Bullion demand in Asia will keep rising as inflation speeds up, HSBC Holdings Plc economists including Frederic Neumann said in a report Oct. 18. Asia and the Middle East accounted for 68 percent of demand in the 12 months to September compared with 8.3 percent for Europe, data compiled by Bloomberg show.
The pace of ETP outflows has slowed to about 40.7 tons a month on average since June, compared with 97.7 tons a month in the first half, according to data compiled by Bloomberg. Holdings fell 28.3 tons this month. The amount held in ETPs reached a record 2,632.52 tons on Dec. 20 last year.
Mine Writedowns
"Most of the 'weak hands' are already out of the market," said Eugen Weinberg, head of commodities research at Commerzbank AG in Frankfurt. "We may see inflows with price stabilization expected from the second quarter of 2014."
Mine output may also drop. The world's biggest producers including Toronto-based Barrick Gold Corp. took at least $26 billion of writedowns, cut spending and fired workers as prices fell and costs rose. UBS estimates miners spend about $1,200 to produce every ounce.
Paulson, the hedge-fund manager who said last year gold was his best long-term bet, told clients last month that he personally wouldn't invest more money in his gold fund because it's not clear when inflation will accelerate, according to a person familiar with the matter.
While Paulson & Co. sold half its stake in the SPDR Gold Trust in the second quarter, the investor remains the largest holder, with 10.23 million shares as of Sept. 30, a Securities and Exchange Commission filing shows.
'Ultimate Bubble'
Soros, who called gold the "ultimate bubble" in 2010, increased his stake in the SPDR Gold Trust over five quarters starting from the three months through the end of September 2011. His company then lowered holdings in the two quarters through March, before selling the remainder in the three months to June, SEC filings show.
"Gold ETFs have fallen out of favor in 2013," said Mike McGlone, the New York-based director of research at ETF Securities, citing the economic expansion, rising equity markets and prospects for tapering by the Fed. "We view 2013 as a correction in the longer-term structural gold bull market."
Copyright 2014 Bloomberg. All rights reserved. This material may not be published, broadcast, rewritten, or redistributed.


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Institute For Geowissenschaften Mineralogie
Institute For Geowissenschaften Mineralogie
Pereira M.F.,University of Évora |
Sola A.R.,National Laboratory of Energy and Geology |
Chichorro M.,New University of Lisbon |
Lopes L.,University of Évora |
And 2 more authors.
Gondwana Research | Year: 2012
The Estremoz Anticline in the Ossa-Morena Zone (SW Iberia) includes upper Ediacaran detrital rocks unconformably overlain by lower Cambrian detrital and carbonate rocks. The spectra of detrital zircon U-Pb ages dominated by Cryogenian and Ediacaran ages (with a typical gap in Mesoproterozoic ages) of the upper Ediacaran greywackes and lower Cambrian arkosic sandstones indicate provenance from sources placed near or at the North-Gondwana margin. These late Ediacaran and early Cambrian basins developed in a paleoposition close to the West African Craton and related to a long-lived Neoproterozoic magmatism (c. 850-700. Ma - Pan-African suture; c. 700-635. Ma - early Cadomian arc; and c. 635-545. Ma - late Cadomian arc).The rhyolites of the Volcanic-Sedimentary Complex of Estremoz whose stratigraphic position so far has been a controversy, yielded an upper Cambrian age (Furongian) at about 499. Ma indicating that carbonate production was episodic in SW Iberia during the Cambrian. This new evidence should be taken into account in the reshaping of paleogeographic reconstruction models that have erroneously insisted on placing Iberia at southerly cold water higher latitudes (>60°S) during the Furongian. © 2012 International Association for Gondwana Research.
Gallastegui G.,Instituto Geologico Y Minero Of Espana Igme |
Gonzalez-Menendez L.,Instituto Geologico Y Minero Of Espana Igme |
Rubio-Ordonez A.,University of Oviedo |
Cuesta A.,University of Oviedo |
Gerdes A.,Institute For Geowissenschaften Mineralogie
Journal of Iberian Geology | Year: 2014
Late Palaeozoic conglomerate formations (Del Ratón and El Planchón) from the Andean Precordillera (Argentina) were studied to unravel their age, composition and provenance. The conglomerates from the Del Ratón Formation are formed by igneous clasts of acid, intermediate and basic compositions (volcanic and plutonic). Laser Ablation (ICP-MS) zircon U-Pb study has yielded an age of 348±2 Ma (late Tournaisian) for the crystallization of a granitic clast, interpreted as a maximum deposition age for the Del Ratón Formation. Geochemistry of these clasts (high LILE/HFSE and La/Yb ratios, negative Nb-Ta anomalies) suggests a calc-alkaline batholithic source, probably located along the Andean Frontal Cordillera currently to the west, where similar calc-alkaline igneous rocks have been described. The El Planchón Formation overlies the Del Ratón Formation and, in the studied conglomerates, there are only igneous clasts of mafic composition (volcanic/subvolcanic). These mafic clasts have a very similar petrography and geochemistry to the Late Ordovician mafic igneous rocks of the Western Precordillera (low LILE/HFSE and La/Yb ratios, no negative Nb-Ta anomalies). Therefore we suggest that the El Planchón conglomerate clasts were probably delivered mainly from northern sources within the Precordillera terrane. This change in clast provenance is tentatively related to a shift in mountain uplift from the Frontal Cordillera (in the west) to the Precordillera (in the east) after the early Visean.


text:
Those who have an inherent distaste for this sort of entertainment will appreciate Conan the Barbarian's impeccable production values without enjoying the story; most everyone else will be swept away by the film's spectacle. show more
I liked a lot of it myself, and with me, a few broadswords and leather jerkins go a long way. show more
A series of meaningless adventures punctuated with a lot of clanky, very bloody swordplay, Conan the Barbarian is best remembered for a scene in which Schwarzenegger punches out a camel. show more


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Discussion in 'Strings' started by pvaudio, Nov 22, 2010.
Haha, and no, you never gave me any myself. They had a "free sample" thing when they first came out.
pv my shopping cart depends on this!
I've got some half sets cut from a reel... We can help each other out.
I hesitate to recommend a multi for hybrids, as the only one I've found to be superb is Xcel FO. Unfortunately, it's near $20 which is natural gut pricing. The gut is going to be superior in all aspects as well.
As a poly, Scorpion doesn't truly compare to anything but itself. For me, it's just one of those game changers. Tour Bite is the spin monster, but is pretty much hopeless everywhere else. B5E is only good as a main. It's hopeless as a cross, and feels less than spectacular in full. It needs something to prop it up. A good multi or gut will do that, and once you've done that, oooooh boy. SpinX is a personal choice for sure. I can't in good faith recommend it to anyone. Typhoon, on the other hand, is another good all around poly. It comes in blue and grey, and while I prefer the blue, many prefer the grey. It's all up to what you want. The blue has more feel, the grey has more spin. The blue is softer, the grey is more responsive.
That helps me a lot.
Have you had a chance to play with Mamba's guts? If so, how do they compare to WhisperTouch?
I see you've used Thunder Blast in a number of your reviews, did you think it was a good cross? Any experience with Microfibre/Micronite or Prince Premiere Attack?
Also what's your advice on tension ratio between polys and multi/gut? Some have sad string the softer 6 tighter, 4, 2, the same etc. Just curious what you've found to be ideal.
Am I reading this correctly? Alien > Alu Rough? Please explain
PS. I hate country Miles, I'm always out of gas...
I don't like ALU. Actually, I don't really like any Lux poly. They just feel quite generic. ALU does have a unique feeling to it, but I don't personally like it. I can, however, see how some people would. Alien though has a great pocketing and bitey feel (yes I just made that word up). Problem is that it's incredibly expensive and simply heathenous to string.
Some new hitting footage. Got done with finals, picked up the sticks, and hit the court in darkness
The backhand is looking better! I like how you are starting to get the racket below the ball some. I'd still love to see a little more front knee bend, as that will help you drive through the ball more.
Thunder Blast is a great cross string and pretty decent in a full bed too.
*ahem* Where, oh where might I find some ThunderBlast? Woe is me!
Much thanks UC!
pv, thoughts on tension for multi/gut in relation to polys? And have you compared Mamba's gut to PerformaXX?
Dear all. I did ask the following question once:"My son of 13 y.o. Playing tennis and after few tests we found out that he prefers guts-poly hybrid and best of all tour bite and b5e.this summer I am going to send him to play few tournaments with a group, where I don't think that he or smb may care about hybrid stringing. So my question is if it is possible to find a good and similar substitution to above setup, but using one string for a full bed". But to get an answer I'd like to simplify it: what is your recommendations for competitive junior who used to nat gut-poly setup to use in a full bed. I need to buy a reel for him before he goes on the trip. So please advise.
On your doorstep come Monday!
I need to check out your new video tonight.
Maybe somebody in here will have an answer but I can't really think of anything. Why is it so important to get just 1 string type?
It is important, because in Asia like Azerbaijan or Georgia ,etc, they don't know anything about hybrid stringing, and while there are just one stringer for the tournament they most likely will refuse to string for you then to undertake hybrid job.
I don't think he has. But sometime over the summer (probably) I'll try Supra and compare it the PmC I'm using now. You could compare the two if you can't wait that long.
I will try to simplify I have enough sets of Tornado, Gamma professional, WC scorpion and TT and b5e, BHBR, MSV Co Focus, SPPP, tour bite, kirschbaum proline II, can get easily Micronite. Racquet used is Volkl O10L, son is playing all court game, trying to come forward as much as possible and enforcing shots when inside court and play with a lot of spin from baseline. So from above list what do you recommend to give him for his journey to be used as a full bed setup? Now he is using nat gut/poly setups in dif combinations.
pv, have you tried out doing a hybrid with any of these polys? Like B5E + CoFocus for example?
You know, honestly, I have not. Although it's the rage in some circles, one of which I won't mention by name, I haven't much cared for any poly/poly hybrids. It's likely because on that front, I agree with Wuppy: a poly is not distinctive enough to be reasonably used as a cross string.
Polys are not just polyester, they are copolyester, polyolfelin ribbon, alum. fluoried resin, etc. To say, "I don't brid poly/poly due to lack of diff. in poly as cross.", is just strange, when everyone on here who has tried more than one poly-copoly-ribbon etc, knows that the differences are huge.
Why do you think that so many have tried so many brids of poly-poly anyway? They are looking for the pop/control/spin/feel combo that you get from vs team/alu or vs/os, without spending the money on vs or they just like poly over gut/alu. My last three sets of vs/alu snapped at grommet shear, and that may have been to the 60lbs, but I doubt it! I was the one who replaced grommets on my pt57a frames, and only about two years ago. So I know the grommet edges are ok. (All were bag snaps.)
For any of you who have followed this guy's advice, on brids, this is a huge sign to stop listening. He does not believe that it is worthwhile to brid poly/poly, period, end of the story. That is an indefensible comment, so then, let's see what he says to defend it.
while i dont like your string of choice ;-) i have to agree with you on this one, some poly's facilitate (lateral) movements better than other, that means if used as cross they will help the main strings grab the ball better.
But I'd say its more worthwhile to spend one's time experimenting with hybrids of different types of string. Maybe if someone knew for a fact that poly/poly was the only option for him/her, then it makes to spend time experimenting in that area in order to fine tune. (And we all know that PV knows about poly and co-poly composition; most people use "poly" to refer to it and its derivatives.)
kiteboard, stick to your own threads please. Stop twisting things that I say around to defend your ridiculous L-Tec agenda. I also did see your stupid white text comment, which I would have assumed that someone of your age would not have to resort to using to make a point. It's really quite immature and getting old extremely quickly.
What hours do you work for GGTennis again?
What's a brid?
This maybe?
i almost hear him saying: hy brid.
Keep on telling people not to brid poly with poly and see how many continue to respect that opinion. Or do you also hate copoly/poly? And poly/ribbon? And poly/copoly? And poly/kevlar? And blah blah anything that you don't do?
Just in case no one has read this.
"No poly is distinctive enough to be used as a cross string." How is that for quoting you correctly? Now that's an immature statement, based on hatred of ltec marketing, or, what else? It can't be based on reality. What that should be screaming to you guys who know better is: This guy does not know what the hell he is talking about.
Mods, if you're out there, would you please mind clearing up all of this garbage from 2621 through this post?
Just so we have the full quote which you decided to edit.
kiteboard, if you want to disagree with me, then that's fine. If you want to repost my opinion elsewhere, that's fine. It speaks for itself and, not strangely enough, is the general consensus in the tennis community: a poly/non-poly hybrid is what more people are looking for than a hybrid consisting of two types of poly. As mixedmedia said, poly is a blanket term for a category of tennis strings much like synthetics are those that are non-kevlar and non-natty gut. If you want to argue me on that point, then you really have nothing better to do.
I did not once tell someone or recommend someone NOT to do poly/poly hybrids. Did that friendly jab in this quote you're gripping onto with your life pass you by? I did not recommend it. I posted my opinion that poly/poly is not what I'm looking for when I know the stringbed feel that I want is going to be found with something multi or gut. And guess what! It always has been. Look at my favorite setups: poly/gut at the top, followed by poly/Xcel. If you want to come in here and derail things based on comments that make you look just that ridiculous, don't bother and kindly refrain from posting in my thread again. It was doing fine without you.
Amidst all this drama, some updates on the 3 being playtested:
BHBR/MCS: Good at first. Once the MCS started to get rough from the BHBR, the spin went down, the mains notched, and now it's not super playable. May cut prematurely.
Tornado/Ex!: Decent at first, better now. Now that it's settled in, it's gotten pretty nice. Nice pop, but still not Xcel.
3S/"Gut": Decent at first, much better now. There is still really not much feel at all, and it's very mushy. The crosses are odd: they have softness only a multi can provide, but are solid to the touch (the mains still slide with ease). Ball fuzz also sticks directly to the multi which is something that only mulitis with specific coatings seem to do. It doesn't matter at all, but PPA did the exact same thing, and every syn gut out there that I've used does that. Good for in between points: pick out the fuzz, good to go. Cross reminds me of Live Wire XP actually. I'll give it a few more hours, but it's just not responsive enough for me.
If I can't find anything once I get my Iontec, I'm just going to take full poly with me and use that. Probably take Tornado and BHBR as I have the most of them.
only one way to solve this like men, fisti cuffs.
also, my friend did not get the gig with wilson, but he still is with alien somehow.
also also, a while ago you did a review on the wilson 90, I can not find the link. Was it in this thread, will you please help me out? Since you have played with my racquet before can you compare them. (might be a different guy, did you say you demoed this before?)
What makes you look ridiculous, is saying that no poly is distinct enough to be used as a cross string, and then defending it as if it's a correct/true/right thing to say. Anyone who listens to that opinion, and agrees with it, fine. Be my guest. The motivation for making it is either honest or not, only you know the truth. For the rest of us who know just what bs it is to say it, and then stand by it, I'd say, take anything this guy recommends, says, with that in mind: His judgment is bad and prejudiced against the truth. Poly/poly brids are the coming dominant force. Right now it's gut/alu, for joker, fed, roddick, murray, etc. Or straight alu or other poly: berdych, tsonga (back and forth from gut/alu rpm) Nadal, tipsarevic, Delpo is full alu. All those guys who use full poly do so because they like it best not because they are paid to. They pay for their string jobs, unless they are like mac, who had to have his money taken out of prize money. (Talk to Drakulie about that.) And then telling the mods to remove your quote and my post regarding it, is also chickencrap. Don't like to have it pointed out, is all. If I were you, I'd just admit it was based on hating ltec marketing, and little else.
I asked the mods to remove all of the posts, except for my original statement. I did that on purpose, because it's simply my opinion to not want to spend time testing poly/poly. So, if people are interested in that sort of hybrid, then they can go to your playtest thread where that's what you do. There is no poly on the market that when crossed with another poly will make it feel like a poly/gut or poly/multi stringbed. That's counter to the purpose of poly aka it's not meant to replicate synthetics or natural gut. Hence: there is no poly distinctive enough to be used as a cross string for me. It's not a set rule, my friend, it's a personal choice. I'm not sure why you keep posting irrelevant things like pros using exactly what I prefer as well, or why I should talk to drakulie about John McEnroe not paying for his stringing at tournaments. You're simply arguing for the sake of arguing.
pv, how much tighter do you recommend stringing multi's in a hybrid?
"What makes you look ridiculous, is saying that no poly is distinct enough to be used as a cross string, and then defending it as if it's a correct/true/right thing to say. "
Looking at ATP and WTA, how many players use poly/poly hybrid? How many use a Poly/something else(gut) hybrid?
There is nothing ridiculous when one's opinion agree with decisions of top players in the world.
Top pro's don't have time to think/research about strings as we do, trust me they go with what they are used to, even now know no one knows for sure if they even use the racquets they are advertised to be using...
I assume you are talking poly/multi hybrids. A four pound increase in tension of the multi seemed to feel good for me.
As Mike just said, I always tend to do the softer string (gut or multi) 3-4.5lbs higher respectively. If it's a syn gut, usually only 2 lbs difference, and if full poly, the crosses I'll do perhaps 1lb. I think I found I liked full Scorpion at 49/50 the best.
Ok great, thanks guys.
While I'm here, what are you opinions on the JET/JayCee method of stringing?
It may be better but I have enough problems trying to remember to change tension between mains and crosses. There is no way I'll get that method right unless I take double the time.
Exactly. I have no reason to want to have a stringjob last me 30 hours without going dead, simply because the strings will break long before then. It's akin to what's going on with alid in his thread. He is using 0s/4s, and even if they didn't go dead in 35 more hours, it wouldn't matter, because they will break long before then. And, in reality, it wouldn't matter, because I play fine with my stringing method and string, and really have no reason to change it.
Very true, but isn't the primary benefit of those stringing methods to increase the actual playability and not just tension/longevity?
I have a dropweight machine. I don't use the proportional part of the JET method, but I do use the string pattern and the pulling method. I skip the 2nd to last main or cross, then I pull extra tension on the 2nd to last string and then tie it off. I also pull tension on the mains for 5 seconds and for the crosses I wait 20 seconds while I straighten the cross and weave the next cross. I end up with a stringbed that is stiffer than the dropweight setting by about 7 pounds at normal tension range (which is consistent with what the JET method is supposed to do).
I did this with PPA and noticed a 9.2% tension loss after 11 hours of play. Last time a friend strung PPA for me on my APDGT, I had 12.5% tension loss after only 5 hours of play (I switched racquets and started stringing on my own after that).
I'm going to keep using this method. It makes sense to me that applying less weight to get the same SBS that you would with a higher setting will result in less stress on the string. I also like the idea of having all your perimeter strings at true tension.
One more thing I want to note is that the tie offs at the mains will undergo more stress than conventional tie offs at perimeter mains, so it's very important to have secure tie off knots. I've been using Parnell knots, but I noticed that on my mains the knots have been slipping slightly. I usually give about a 1/2" of length when I make my cuts but now there is almost no extra length on the knots. I'm going to use double half-hitch knots next time to see if they work any better, or I might try using a PC knot on one of them.
^^^ I'd be interested to know if leaving a string to tension for 20secs was much different from using the pre-stretch function. But that might be throwing this thread off topic...
If you're talking about the pre-stretch function on an electronic machine, the 20 sec wait is much different because you never add additional tension. The concept is that I can get a SBS of 57 by setting my calibrated drop weight to 50, and that puts less stress than pulling it at a higher tension with a lockout machine. If you have an electronic CP, then you can do the same thing by waiting 20 secs.
Yeesh. lot of quoting here, I've been MIA
I really liked PHT/Red PL II years ago. It softened up the PHT just a touch. I think you explained yourself much better later on though-- nothing is going to play like a multi/gut cross.
What's with the white text-- Seriously? Frankly, I think this was a bit of an extreme reaction.
In regards to the second PP. As someone who has tried more than plenty poly hybrids; No, we're not looking for the "gut" replacement. It simply doesn't exist.
Re: gut snapping. VS doesn't snap @ 70# (although the few times I've strung it there, I've prayed to the stringing saints on every pull). 60# isn't an issue at all. Are they snapping in the same frame or 3 different frames? Check your grommets again-- grommet quality can change from string job to string job or even in a hit. I have a p.i.t.a. customer who snaps aeropro groms every week or so-- I got so sick of replacing them that I just started tubing the heck out of his frames. Either you have a bum grommet, or your stringer isn't paying attention. The last, although highly unlikely, possibility is that you got bum sets of VS from the same batch.
Iontec Black has this strange quality-- the string feels "soft" in the hands but the physical string is rather hard. Bomber and 0s seem to share the same characteristic (although both of these are definitely much stiffer) as well and it seems to stave off notching. To date, I I don't think I've been able to notch and pop a set of bomber-- it's borderline ridiculous.
I have a reel of Iontec Black 1.25, shoot me an e-mail if you would like to try some.
I forgot what you're hitting with but shoot me an e-mail and I can give you a rundown of the 90s if PV is busy.
I've come to find that it's an acquired taste. I seem to like it and since I enjoy stringing, I don't mind putting in the extra time to fiddle around. On the other hand, some players that like more "response" and direct feel from the stringbed aren't terribly fond of it-- they're used to what they have and don't like change.
The biggest thing you'll find is that the strings will play a touch more forgiving or "softer" than @ comparable tension while feeling like they're strung a lb or two higher. It's a strange paradox
Ramon prettymuch hit it on the head. What I most enjoy about this is the pattern-- I use it even for regular customers without changing tensions etc. It makes sense-- Even if you discount the extra "resistance" created by the skip, you can clamp off closer to the grommets and tie off closer to the last main pulled which translate to less loss in tension.
I'm not so sure about the tie-off situation. So far I've had no issues stringing poly, multi, or gut this way and I snip my tie-offs very, very, short.
Separate names with a comma.


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I have a cookie tin (the type you get at christmas) for all my little stuff. keeps it right handy and it fits under my clear extension.
Hope I'm posting this tip correctly and I don't know if anyone else has come up with a similar idea...When I was working on a sampler quilt with each block a different design I wanted some way to make sure I had all my pieces cut out correctly and they looked OK. I had half of a foam core board (you know, the stuff they use for science fair presentations). I split that in half and at an office supply store found hinges that are specifically designed to join that the type of board. I put the hinge in, covered the board with white fleece ( could use felt also) which I attached to the back with hot glue. Now I had a design board the cut pieces would stick to and if I was in the middle of a block and had to put things away, all I did was fold the board at the hinge and everything stayed where it was until I could get started again.
Thats a good one, i'll remember that one. Science fairs are not in my school life what type of hinges do you use? most of use use tape etc.
My tip is for marking your fabric in the Quilt Store when you buy it. I always have painting tape (Blue Tape) in my purse. I buy the fabric for my pattern and as soon as it is cut I write on the Painter's Tape what it is for. ...or Fabric A, Fabric B, Fabric C.....and stick it onto the fabric. Now, when I get home I know exactly what each piece of fabric is for....I get to turn off my brain!
Hey thats a very good tip, surely I will try and let you know...
I love marking my quilt tops with a chalk pencils, but because they are so fragile, sharpening them can be a challenge. I've discovered the best tool for sharpening my chalk pencils is a sharpener that you would use to sharpen make up pencils, such as as an eye brow pencil. The sharpener is designed to work with a more delicate pencil, and I've never had problems with my pencil "lead" breaking as I tried to sharpen it.
Coffee filters make a great template for circle cuts, and can act as stabilizer. They work great for piece work too!
I have been quilting for 30 years, and this is unorthodox, but works for me . . . . . rather than make hanging sleeves for wall hangings, I use the "Stapler and Clam Knife" method!
Position the staples about 5 or 6 inches apart. Hide the staples between the binding and border, and the clam knife (which has a blunt end) is used in case I need to reposition the staple, as it won't tear the fabric. This makes a nice flat wall hanging and I have been doing it for years!
Wow, so many great ideas are here. I have a cat who believes she is a binding expert. When I begin to wind the binding on the paper towel cardboard center, she perks up and comes to help. As the binding comes off the roller, she grabs it and really increases the tension. To stop her from helping me so much, I have begun giving her a roller of her own. I stapled a long strip of assorted selvege and fabrics on a wooden dowel. Adding some heavy duty fishing swivels to the ends and attaching it to the back of my chair she is content to use it while I sew. During a sewing break, I re-wind the fabric and she is occupied until the next break. So far it has not gotten old for her.
Why not just finally make up your mind which quilt you are going to do and JUST DO IT!
Barbara Daniels
Houston, Texas
If you have trouble matching points when sewing blocks together, put a small dab of water soluble glue stick on the fabric where the points are to join and press them together. Be sure to put it only on the side where the seam allowance will be. Sew your quarter inch seam, and you will have perfectly matched points! When the quilt gets washed, the glue will wash out.
Excellent tip. I do the same with the washable glue when making blocks with curved seams, i.e., drunkards path, New York Beauty, etc. Three little swipes with the glue stick--one at each point and one in the middle--and I've never had to pin!
If you like the look of scrappy quilts, but don't have the confidence to pick the colors, just select a dozen fat quarters of fabric collections, and use them. The quilt will always look good if the fabrics look good together.
If you use the finger cots to hold the needle when sewing the ones in the cataloges are expensive, buy them through your medical supply company a gross (144) less then $5.00
Hopefully I don't duplicate my entry. I lost everything I typed - due to operator error!! LOL.
First....I tend to cut out an entire quilt at a time. I put all pieces into zip-lock bags and label them with sharpies. All pieces and instructions are then put into "shoe-box" plastic boxes that I have. (My husband and I were lucky enough to end up with about 100 of them when he was cleaning out a warehouse!). You could use any type of storage container from a discount store. I have at least 7 quilts cut out right now. This way, if I have fabric that I have specifically tagged for a certain quilt - there is no possible chance that I will use it for something else!! I always try to do one of the pre-cut quilts before I start something new.
Secondly...Regarding cats and cat hair....unfortunately you may think...our cat is not allowed in my sewing room. Not that I have anything against him, he sleeps on my chest most every night, but, I sew a lot of baby quilts and I have had experiences where cat allergies have been an issue. So...my tip...I made a quilt, specifically for the cat and placed it right outside the sewing room door, the cat will lay on that quilt and watch me while I'm sewing. If he tries to sneak in I send him out. And also the door to the sewing room stays shut at all other times, so that he cannot get in and get hair on anything. I can feel comfortable that there is no cat hair on any blankets that I provide to anyone. And he can get as much cat hair on his quilt as he wants too!!! So everybody is happy!
One quilting project, like one cookie, is never enough.


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It is well known that muscle contraction and insulin can independently translocate GLUT-4 from an intracellular depot to the plasma membrane. Recently, we have shown that the fatty acid transporter FAT/CD36 is translocated from an intracellular depot to the plasma membrane by muscle contraction (<30 min) (Bonen et al.J Biol Chem 275: 14501–14508, 2000). In the present study, we examined whether insulin also induced the translocation of FAT/CD36 in rat skeletal muscle. In studies in perfused rat hindlimb muscles, we observed that insulin increased fatty acid uptake by +51%. Insulin increased the rate of palmitate incorporation into triacylglycerols, diacylglycerols, and phospholipids (P < 0.05) while reducing muscle palmitate oxidation (P < 0.05). Perfusing rat hindlimb muscles with insulin increased plasma membrane FAT/CD36 by +48% (P < 0.05), whereas concomitantly the intracellular FAT/CD36 depot was reduced by 68% (P < 0.05). These insulin-induced effects on FAT/CD36 translocation were inhibited by the phosphatidylinositol 3-kinase inhibitor LY-294002. Thus these studies have shown for the first time that insulin can induce the translocation of FAT/CD36 from an intracellular depot to the plasma membrane.This reveals a previously unknown level of regulation of fatty acid transport by insulin and may well have important consequences in furthering our understanding of the relation between fatty acid metabolism and insulin resistance.
- hindlimb perfusion
- glucose transporter 4
- palmitate
- oxidation
- triacylglycerol
- diacylglycerol
- phospholipids
skeletal muscle is an important target for insulin action, because this hormone has multiple effects on muscle intermediary metabolism. One of the best known insulin effects is the stimulation of glucose transport through the translocation of GLUT-4 from intracellular vesicular structures to the sarcolemma. Insulin is also able to regulate fatty acid metabolism in the myocyte. For example, in skeletal muscle, insulin can inhibit fatty acid oxidation through the inhibition of carnitine palmitoyltransferase I, an effect that is mediated through increased intracellular levels of malonyl-CoA (4, 14). It has now also been shown that insulin increases fatty acid esterification into triacylglycerols in skeletal muscle while simultaneously reducing fatty acid oxidation (13, 22). From these studies, it can also be inferred that insulin increases net fatty acid uptake into the myocyte, since the insulin-induced increase in fatty acid esterification was markedly greater than the insulin-induced inhibition of fatty acid oxidation.
Fatty acid uptake across the plasma membrane occurs via both diffusion (17) and a protein-mediated mechanism (2), involving a 43-kDa plasma membrane fatty acid binding protein (19) and an 88-kDa heavily glycosylated fatty acid translocase, the rat homolog of human CD36 (FAT/CD36) (1). The 63-kDa fatty acid transport protein (FATP1) (24), originally also thought to be a fatty acid transporter, may not be involved in the transsarcolemmal transport of fatty acid, since it has been shown that FATP1 is a very long chain fatty acyl-CoA synthetase (9, 26). We (6) have recently found evidence that FAT/CD36 is involved in the short-term regulation of fatty acid uptake by muscle; namely, during muscle contraction (<30 min), fatty acid uptake by muscle is increased. This was shown to be due to the translocation of FAT/CD36 from intracellular compartments to the plasma membrane, since the blocking of the plasma membrane FAT/CD36 with sulfo-N-succinimidyl oleate prevented the contraction-induced increase of fatty acid uptake (6). Thus fatty acid uptake can be acutely regulated through the subcellular redistribution of FAT/CD36.
Whether FAT/CD36 distribution within the myocyte is also regulated by insulin is not known, but because insulin increases net fatty acid uptake (13, 22) via a phosphatidylinositol (PI) 3-kinase-dependent mechanism (13, 22), and because it it is well known that insulin induces the translocation of the glucose transporter GLUT-4 via PI 3-kinase activation, it is reasonable to hypothesize that insulin increases fatty acid uptake by muscle by translocating FAT/CD36 from its intracellular compartments to the plasma membrane. Therefore, in the present studies, we have examined the effects of insulin on 1) fatty acid uptake and metabolism in perfused rat skeletal muscle and 2) on the translocation of FAT/CD36 from its intracellular compartment to the plasma membrane. Our results show 1) that insulin stimulates fatty acid uptake by 51% in intact muscles, and 2) that this is closely matched by the insulin-induced increase in plasma membrane FAT/CD36 (+48%) and concomitant reduction in the intracellular FAT/CD36 pool (−68%), effects that were blocked by the PI 3-kinase inhibitor LY-294002.
Fat-free BSA was obtained from Roche Diagnostics (Laval, QC, Canada). [1-14C]palmitate was obtained from Amersham Life Science (Oakville, ON, Canada). LY-294002 was obtained from Calbiochem. Human insulin (Humulin) was purchased from a local pharmacy.
Male Sprague-Dawley rats were used in all experiments (200–250 g). Animals were housed in a controlled environment on a 12:12-h light-dark cycle and fed Purina rat chow ad libitum. All procedures were approved by the animal care committee at the University of Waterloo. Animals were anesthetized with an intraperitoneal injection of pentobarbital sodium (6 mg/100 g body mass) before all experimental procedures.
Hindlimb perfusion.
Rat hindlimb muscles were perfused for 60 min in either the absence or presence of insulin (20 mU/ml) with the use of a cell-free perfusate that contained palmitate (1 mM) and 4% BSA, as we have described previously (18, 20) in detail. In this perfusion model, we have shown that insulin increases glucose transport and GLUT-4 translocation to the plasma membrane (18, 20). Muscles were also perfused with PI 3-kinase inhibitor LY-294002 (60 μM) and with insulin + LY-294002. This concentration of LY-294002 maximally inhibits insulin-stimulated glucose transport in perfused rat muscle (28). Muscles from all of these experiments were used to fractionate rat hindlimb muscles (seeSubcellular localization of transporters in skeletal muscle).
In separate experiments, we also determined basal and insulin-stimulated palmitate (1 mM) oxidation rates in the rat hindlimb by collecting arterial and venous effluents at regular intervals during the perfusion and measuring the 14CO2 in these samples by means of a modification of previous procedures used in our laboratory (10-13). In addition, palmitate incorporation into phospholipids, triacylglycerol, and diacylglycerol was determined by measuring [14C]palmitate accumulation in these depots in selected rat hindlimb muscles (soleus and red and white gastrocnemius) as we have previously reported (10-13). This permitted us to calculate the incorporation rates of palmitate in the whole rat hindlimb musculature from the known muscle fiber composition of these muscles and the known proportions of their muscle fiber mass in the rat hindlimb (3), as we (20) have done previously when determining glucose transport for the whole hindlimb muscle from selected individual muscles.
Subcellular localization of transporters in skeletal muscle.
Muscles were fractionated as we (6) have recently described to determine the subcellular location of FAT/CD36. Briefly, muscles (1.5 g) were minced for 5 min in 10 mM NaHCO3 (pH 7.0), 0.25 M sucrose, 5 mM NaN3, and 100 mM phenylmethylsulfonyl fluoride (buffer A). The minced muscles were homogenized (1 g/15 ml dilution) using a Polytron (Brinkman Instruments) at a low setting of 3 for 5 × 1 s. The resulting homogenate was centrifuged at 1,300 g for 10 min. The supernatant was saved, and the low-speed pellets were resuspended in buffer A (2 g/15 ml), homogenized, and centrifuged again. Both supernatant fractions were pooled. The low-speed pellet was resuspended in 0.5 M LiBr, 50 mM Tris (pH 8.5), and 100 mM phenylmethylsulfonyl fluoride (buffer B) at a ratio of 1 g tissue/25 ml and stirred for 4 h. The pooled 1,300-g supernatant fractions were centrifuged at 9,000g for 10 min. The 9,000-g pellet was saved, and the supernatant was spun at 190,000 g for 1 h. The 190,000-g pellet was resuspended in 800 ml of buffer A by five strokes with the use of a tightly fitting Potter-Elvehjem glass homogenizer and subjected to a continuous Percoll gradient as described below. The LiBr-treated membranes were centrifuged first at 1,200 g for 5 min and then at 10,000g for 30 min. The LiBr-10,000-g pellet was saved. The supernatant fraction was centrifuged at 190,000 g for 1 h. The LiBr-190,000-g pellet was resuspended in 1.2 ml of buffer A by 40 strokes with the use of a tightly fitting Potter-Elvehjem glass homogenizer. Five hundred milliliters of both the 190,000-g pellet and the LiBr-190,000-gpellet were layered on top of a 7-ml Percoll suspension (60%) in 120 mM KCl, 25 mM HEPES (pH 7.0), 5 mM NaN3, and 0.5 mM EGTA (initial density 1.12 g/ml) resting on a cushion of 1 ml of 1.25 M sucrose. The resulting gradients were centrifuged at 62,000g for 1 h. After centrifugation, 0.5-ml fractions were collected starting from the top. All procedures were performed at 0°C. The fractions were stored at −80°C and, upon thawing, were used for SDS-polyacrylamide gel electrophoresis followed by Western blotting. Before electrophoresis, Percoll particles were removed by alkalinizing aliquots of the fractions to pH 12.0 by the addition of NaOH, followed by centrifugation in a microcentrifuge for 2 min. The supernatant was then neutralized to pH 7–8 by the addition of HCl. In the resulting Percoll-free fraction samples, the protein content was determined by the bicinchoninic acid method, and 5 mg of each fraction were subjected to SDS-polyacrylamide gel electrophoresis and Western blotting. For detection of FAT/CD36, the antibody MO25 was used (21). The GLUT-4 was detected with a commercially available antibody (East Acres Biologicals). The monocarboxylate transporter MCT4 was detected by using an antibody prepared in our laboratory (7).
Statistical analyses were performed using analyses of variance andt-tests where appropriate. All data are presented as means ± SE.
In the perfused rat hindlimb muscles, insulin increased the incorporation of palmitate into skeletal muscle phospholipids (+29%,P < 0.05), triacylglycerols (+80%,P < 0.05), and diacylglycerols (+49%,P < 0.05). Concomitantly, insulin reduced palmitate oxidation (−20%, P < 0.05; Fig.1). We can ignore the palmitate incorporated into monoacylglycerols, because that typically represents <1% of the palmitate taken up by muscle (10, 12). Similarly, free palmitate in muscle was not determined, as the unesterified fatty acid content in muscle is negligible (25). Thus, in rat hindlimb muscles, the net uptake of palmitate was increased 51% (P < 0.05; Fig. 1) by insulin.
These observations in the perfused rat hindlimb muscles are consistent with a recent report from our laboratory, which also demonstrated that insulin increased palmitate incorporation into phospholipids, triacylglycerols, and diacylglycerols, and reduced fatty acid oxidation in isolated soleus muscle (13). In these studies (Fig. 1and Ref. 13), insulin increased the total fatty acid taken up by the muscles to a similar extent [+51% in perfused muscles and +43% in isolated soleus muscles (13)], since the insulin-stimulated increase in fatty acid esterification was much greater than the concomitant reduction in fatty acid oxidation (Fig.1).
The insulin-induced increments in fatty acid uptake are not attributable to changes in the delivery of fatty acids, because1) in perfused muscles (present study) the perfusate flow is controlled, 2) in isolated muscle preparations (13) the muscle is maintained in a constant volume of buffer, and 3) in both preparations the fatty acid concentration remains constant. However, we (6) have recently reported that the fatty acid transporter FAT/CD36 can be translocated from an intracellular pool to the plasma membrane by muscle contraction to meet the increased metabolic demand of the contracting muscle (6). Therefore, we speculated that the insulin-induced increase in fatty acid uptake by intact muscles, when the fatty acid delivery remained constant, may also be attributable to the insulin-stimulated translocation of the fatty acid transporter FAT/CD36.
When the muscles were perfused with insulin, the plasma membrane FAT/CD36 was increased by 48% (P < 0.05, Fig.2, A and B) whereas the intracellular FAT/CD36 was reduced by 68% (P < 0.05, Fig. 2, A and B). Insulin also translocated GLUT-4 in the perfused muscles (Fig. 3), as we have shown previously in perfused muscles (18, 20). The monocarboxylate transporter MCT4, which is also present at the plasma membrane and in an intracellular pool (7), was not translocated by insulin (Fig. 4). When the muscles were perfused with insulin along with the PI 3-kinase inhibitor LY-294002, no changes in FAT/CD36 redistribution were observed (Fig. 2, A and B). GLUT-4 appearance at the plasma membrane was also blocked when LY-294002 was included with insulin in the perfusate (Fig. 3). This inhibition of insulin-stimulated GLUT-4 translocation by LY-294002 confirms previous observations in skeletal muscle (27) and adipocytes (8). In addition, we (13) and others (22) have demonstrated recently that the insulin-induced alterations in fatty acid metabolism are prevented when the activity of PI 3-kinase is inhibited by either wortmannin (22) or LY-294002 (13).
The novel observation in the present study is that insulin can induce the translocation of FAT/CD36 from an intracellular depot to the plasma membrane, involving a PI 3-kinase signaling step. Thus this hormone will simultaneously translocate GLUT-4 and FAT/CD36. Also, it appears that insulin signaling of FAT/CD36 (present study) and GLUT-4 translocation in muscle (15, 20) occur via the same early postreceptor signaling steps. Moreover, both FAT/CD36 translocation (6) and GLUT-4 translocation (15, 20) can also be provoked by muscle contraction. Whether there are separate signaling pathways for inducing the translocation of FAT/CD36, as has been shown for the translocation of GLUT-4 by insulin and muscle contraction (16, 20), is not known. But in recent studies in our laboratories, we have shown that insulin and muscle contraction additively stimulate fatty acid uptake into intact isolated muscles (13) and cardiac myocytes (J. J. F. P. Luiken, J. F. C. Glatz, and A. Bonen, unpublished data). Thus these observations suggest that there may also be insulin-sensitive and contraction-sensitive intracellular FAT/CD36 compartments in muscle. This is currently under investigation.
By increasing FAT/CD36 at the plasma membrane, the rate of fatty acid uptake by muscle is increased (5, 6). Thus the insulin-induced increase in the rate of fatty acid esterification (Fig.1) is attributable, in part, to the insulin-induced translocation of FAT/CD36. This is also suggested by the similar insulin-induced increases in plasma membrane FAT/CD36 (+48%) (Fig. 3) and the total fatty acid uptake (+51%) by the perfused muscles (Fig. 1).
The regulation of fatty acid uptake and metabolism by insulin may have considerable implications for furthering our understanding of insulin resistance in muscle. It has been shown that insulin resistance in this tissue is positively related to the the intramuscular content of triacylglycerol (23). Because insulin stimulates fatty acid uptake and esterification to triacylglycerols (Fig. 1), the hyperinsulinemia observed in animal models of insulin resistance (e.g., obese Zucker rats) may promote fatty acid uptake due to an increase in plasma membrane FAT/CD36. Indeed, we now have evidence for this (J. J. F. P. Luiken and A. Bonen, unpublished data). This permanent relocation of FAT/CD36 to the plasma membrane is then directly linked to an increased rate of fatty acid uptake and a progressive accumulation of intracellular triacylglycerol depots.
In summary, we have shown that insulin stimulates FAT/CD36 translocation from an intracellular compartment to the plasma membrane in skeletal muscle. This reveals a previously unknown level of regulation of fatty acid transport by insulin and may have important consequences for furthering our understanding of the relation between fatty acid metabolism and insulin resistance.
This study was supported by the Canadian Institutes of Health Research, The Natural Sciences and Engineering Research Council of Canada, and the Netherlands Heart Foundation (D90.003).
J. J. F. P. Luiken is a Dekker postdoctoral fellow of the Netherlands Heart Foundation.
Address for reprint requests and other correspondence: A. Bonen, Dept. of Kinesiology, Univ. of Waterloo, Waterloo, ON, Canada N2L 3G1 (E-mail: abonen{at}healthy.uwaterloo.ca).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2002 the American Physiological Society


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10 quality Multiple Sclerosis PLR Articles with private label rights. The articles are in plain txt file format for easy use.
Includes the following titles and word counts:
Coping Techniques For People Suffering From Multiple Sclerosis – 554
Facts Surrounding Multiple Sclerosis – 566
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Product Rights:
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Update August 10, 2017: After publishing this article I reopened the Trac ticket arguing the reason for closing it was no longer valid in lieu of Gutenberg's collection of telemetry. A few hours later it was announced that usage tracking will be removed from Gutenberg and the ticket was closed as the original argument for closing it is once again accurate.…
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My Inbox is flooded these days with jokes on Steve Bucknor, Andrew Symonds and some others.
So I thought of posting some of them on this blog...and here they are.
Now, some of these jokes might seem racist or something...but then, it is all in good humour and they are quite funny too!! Laugh away.....
Why Bhajji was banned....
Procter: Did you call Symonds a Monkey?Spot the monkey contest!!
Bhajji: No, I called a monkey, Symonds.
Procter: You did what?!!
Bhajji: (Silence)
Procter: How dare you insult a monkey. You are banned for 3 matches!!
Bhajji: (Sad)
Ponting& Co:!!! ?
Ponting: Hey, Symonds.What did he say?
Symonds: Whatever, he is banned. You can bat well next game.
Ponting: No, Anil is there too, ask Mike if he too can be banned too.
Symonds: We'll see in the next match. If he takes your wicket, we'll complain.
Ponting: Fine then. Come.
Rare pic of Steve Bucknor


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I am a decent poker player. However, for the life of me, I struggle to figure out how to win consistently against the lesser-skilled players I frequently encounter in the small stakes games I play.
I often think to myself, "I am the best player at this table — why am I not crushing?" It's like there is some unseen force that I do not understand that is designed to keep me from discovering the truth. Let's just call it… the Matrix.
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text: severe to extreme speech complications, impairment, dysfunction or diminish, demolish of, with your speech, DVT/Blood Clot(hospitalize for a week; put on warfin, and given low dose heparin in the hospital), sharp pains in the upper right arm with numbness; every so often, latose, foods, drugs intolerance/reactions, and mild to severe emotional distraughtment! The only medications that were taken: warfin, lisinpril, amlopine, and hydrocotozone mix with APAP! Currently not taking any medications at all; except for pain headache- ibuprofen!


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