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Marketing Intern
Internship DescriptionThe candidate should fulfill the following requirements:- 1. Good communication skills. 2. Willing to go out of the way to achieve objectives. 3. Ability to think creatively. 4. Perseverance.
ResponsibilitiesJob Responsibilities:- 1. You would be approaching two different analysts, convince and get them to register on the Tipbazaar.com. (Vendor acquisition) 2. Doing the market research, collecting the data. 3. Real-Time testing the working of the portal and reporting the potential changes. 4. Analyzing the sales process of the Financial market and implementing the process to acquire the customers. 5. Creating and implementing the marketing strategies.
About Company
TipBazaar is a world's 1st Innovative platform which gives a new trend to the Stock Market Trading system. TipBazzar gathers all the Tip Providers (Analysts) onto a single platform and will compare their performance which ultimately will help Subscribers of the Tips.Premium Tip Providers in the market, who charges a certain amount for their daily tips. These tips can be accurate or can fail, but all the time investor's money is at stake which can result in Huge Loss to the investors.
TipBazaar have a different back-end where Tip Providers will feed their premium tip and our platform will check if Tip has got Failed or it has Hit the Target and according to its Performance TipBazaar will give Analyst's Success Ratio, Accuracy Ratio, ROI and Ranking.
Subscribers will also be able to check the past performance of the Analysts sector wise, stock wise and also with the package values.TipBazaar is a Win-win platform to both the Tip Providers (Analysts) and Subscibers


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3 Zamia Way Gracemere 4702 QLD
This spacious home has all the boxes ticked.
* Four good sized bedrooms, main with air con and ensuite and WIR - tick
* Large tiled living area - tick
* Great kitchen with heaps of bench and cupboard space - tick
* 2 car accommodation - tick
* fully fenced yard and outdoor area - tick
**Contact us on 4922 2140 or at [email protected] for an application or to arrange a viewing. Outside pets subject to approval.**
Property Code: 262
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subset: commoncrawl


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Does racial bias fuel Obama foes? The question of whether race fuels opposition to President Barack Obama has become one of the most divisive topics of the election. It is sowing anger and frustration among conservatives who are labeled racist simply for opposing Obama's policies, and liberals who see no other explanation for such deep dislike of the president.
It's impossible to believe that race is fueling every so-called "Obama foe," some of whom are black themselves, and many of whom certainly just don't like the president's policies, regardless of his race. Even some people who vociferously dislike Mitt Romney are finding themselves unable to cast a ballot for Obama because of all the innocent blood drawn under his command. That said, if conservatives are finding themselves angered over the accusations that there are in their ranks a number of outspoken racists, perhaps they should direct that anger toward the outspoken racists in their ranks.
Here is an abridged list of some of the most prominent racist attacks on Obama in the last year (several have come in the last few weeks).
1. A Texas woman woke up last week to find that the Obama sign in her front yard had been made to look like this:
2. Also last week, but far away from Texas, in the quaint New York hamlet of East Hampton, 63-year-old Lawrence Nickel was arrested and charged with a four-month anti-Obama graffiti spree. Amongst the elderly man's other tags was this one, scrawled on a bench: "Obama is a lying nigger."
3. This week the Virginia GOP had to ask its Mecklenburg County affiliate to remove from its Facebook page pictures of President Obama depicted as a thug, a caveman, and a witch doctor.
4. Earlier this month, civil rights groups said they planned to demonstrate a Marietta, Georgia, bar whose sign read, "I heard the White House smelled like collard greens and fried chicken." Earlier, in the same state, another bar owner came under fire for putting this on his sign: "I do not support the nigger in the White House."
5. In March, a woman named Paula Smith came up with this punny anti-Obama bumper sticker:
6. In December, a right-wing candidate who'd lost a bid for city council in Carson, California, tweeted of Obama, "Assassinate the f*ckin n*gger and his monkey children."
Note that these are just the ones the press found out about, and then ask yourself: "Might race be fueling some of the opposition to President Obama?"
Source: Naija.ng


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Grand Rapids, MI (July 18, 2014) – A Grand Rapids-area special education teacher has filed state charges against a local union and the school district for violating Michigan's Right to Work law.
With free legal assistance from National Right to Work Foundation staff attorneys, Becky Lapham of Portland, Michigan, filed the state charges with the Michigan Employment Relations Commission (MERC) in Detroit.
The 11-year Lincoln Developmental Center school teacher filed charges against the Michigan Education Association (MEA) union and Grand Rapids Public Schools for entering into an agreement illegally amending and extending the forced dues provisions in the monopoly bargaining agreement beyond the date allowed under Michigan's Right to Work law for public employees.
That law specifies that contracts or amendments entered into after the law went into effect must respect workers' right to refrain from the payment of any union dues or fees.
Furthermore, Lapham's other charge filed in April, after MEA union officials refused to acknowledge her unequivocal right to refrain from union membership, is still pending with the MERC.
"Across the state, union officials are pulling out all the stops to keep workers from exercising their rights under Michigan's Right to Work law," said Mark Mix, President of the National Right to Work Foundation. "Foundation staff attorneys are assisting workers throughout the state whose rights under Michigan's Right to Work law are being denied by unscrupulous union officials seeking to circumvent the law."
The National Right to Work Legal Defense Foundation is a nonprofit, charitable organization providing free legal aid to employees whose human or civil rights have been violated by compulsory unionism abuses. The Foundation, which can be contacted toll-free at 1-800-336-3600, assists thousands of employees in more than 250 cases nationwide per year.


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This download pack is available to those who contributed to our 2015 H.P. Lovecraft Film Festival & CthulhuCon, Portland, OR Kickstarter campaign. Backers were sent a code to allow download of the pack. If you have a code, simply add this product to your shopping cart and go through the checkout process, using the special code in the coupon code field during checkout. The digital download contains:
"Shhh" exclusive track by The Darkest of the Hillside Thickets
"Flesh and Bone" audio track by Roadside Memorial
"Deep Ones at a Science Fair" the 2015 Pickman's Apprentice theme. Pieces were created by Mike Dubisch (winner), John Donald Carlucci, and Heather Hudson.
Mythos artwork by John Donald Carlucci.
Check out his "Kid Lovecraft" comic in the next issue of Strange Aeons Magazine!
Desktop Images and pages from the MU R'lyeh Expedition Log book
Adorn your desktop with images from the R'lyeh Expedition.
PDF of the 2015 H.P. Lovecraft Film Festival & CthulhuCon Daily Lurker Festival Program
Contains the 2015 Festival program information, plus an original story by Molly Tanzer, articles by Dominique Lamssies and Pete Rawlik, Lovecraftian comic strips by Dark Horse Comics, and more!
This collection of audio and visual treats was compiled for YOUR EYES ONLY! We hope you enjoy it, but please remember these creators worked hard on this stuff, and have given us permission to include them. All materials are copyright of the artists & musicians. Please don't use or redistribute these works without permission. Any images in the Desktop_and_Facebook_images folder are okay to use on your Facebook or computer desktop.


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Brookfield looking to boost holdings on Ninth Street NW
Brookfield Office Properties is close to a deal to acquire 799 Ninth St. NW for more than $100 million, potentially priming the downtown D.C. office tower for a redevelopment as most of its tenants prepare to move out for new space in the District's NoMa area.
The acquisition would be Brookfield's 12th property in downtown D.C. and its third in the same block, where it also owns 701 Ninth St. and the Victor Building at 750 Ninth St.
New York-based Brookfield has a contract to buy 799 Ninth from AEW Capital Management LP, which paid $127.5 million, or about $634 per square foot, for the 201,464-square-foot building in 2006, real estate sources say. The building could trade for between $100 million and $120 million, sources say, putting it at between $496 and $596 per square foot.
Greg Meyer, senior vice president for Brookfield's Washington region, declined to comment.
Brookfield beat out several interested bidders for the 10-story building, including District-based Douglas Development Corp., a sign some real estate experts say bodes well for the strength of D.C.'s office market.
CBRE Group Inc. has been marketing the building. CBRE Executive Vice President Bill Kaye declined to comment.
Several government agencies, including U.S. Customs and Border Protection, are slated to move out of the building later this year for new offices under construction at Trammell Crow Co.'s Sentinel Square I. Those moves will leave 799 Ninth, built in 2001, mostly vacant.
That there was such robust interest in the building given its pending vacancies shows that there is demand for value-add plays by leasing the building to new tenants at potentially higher rental rates, said Scott Homa, Mid-Atlantic research director for Jones Lang LaSalle Inc.
The sale would add to what has so far been a modest year for investment property sales in the District. About $1.1 billion in deals were completed in the first quarter of 2012, down from about $1.7 billion in the first three months of 2011. Some of those deals this year included N.S.P. Ventures Corp.'s acquisition of 801 Ninth St. NW for about $625 per square foot and Paramount Group's purchase of 2099 Pennsylvania Ave. NW for about $767 per square foot.
Brookfield Office Properties is part of the larger Brookfield Asset Management, which also holds a large stake in mall owner General Growth Properties Inc. and residential developer Fairfield Residential LLC. The company recently disclosed plans to bundle several of its divisions, including Brookfield Office Properties, into a new subsidiary to be called Brookfield Property Partners. The new entity would trade under a separate stock ticker, BPY, if approved by financial regulators.


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GraphicRiver Steam Roll Shipping and Logistics Logo 9009233
Category: Logo Template | Comment: ( 0 )
GraphicRiver Steam Roll Shipping and Logistics Logo 9009233
Logo Template \ Humans
Train \ Logistics \ Steam \ Retro \ Railway \ Railroad \ Rail \ Transportation
Logo Template - Steam Roll Shipping and Logistics Logo 9009233 by patrimonio @ GraphicRiver
Logo Template \ Humans
Graphics Files Included: Vector EPS, AI Illustrator; Layered: Yes; Minimum Adobe CS Version: CS; Resolution: Resizable.
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100% re-sizeable vectors. Logo available in vector EPS and AI formats.
Fonts and color easy to customize.
Fonts used: Trajan Pro and
Adobe Garamond Pro Italic (system font)
Thoughtweld Precision Engineering Logo; Biomech Vitamins Logo; Blast Industries Sandblasting Logo; Sunrise Fried Chicken House Logo; Rowdy Rooster Fried Chicken Logo; Fidelity Vintage Film Photography Logo; Radiate Fumigation Logo.
Keywords: Shipping and Logistics, Steam Roll, artwork, circle, engine, freight, graphics, illustration, isolated, locomotive, logistics, logo, rail, railroad, railway, retro, steam train, train, transport, transportation, travel, traveling, vintage.


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Retinal degenerative diseases, such as glaucoma and macular degeneration, affect millions of people worldwide and ultimately lead to retinal cell death and blindness. Cell transplantation therapies for photoreceptors demonstrate integration and restoration of function, but transplantation into the ganglion cell layer is more complex, requiring guidance of axons from transplanted cells to the optic nerve head in order to reach targets in the brain. Here we create a biodegradable electrospun (ES) scaffold designed to direct the growth of retinal ganglion cell (RGC) axons radially, mimicking axon orientation in the retina. Using this scaffold we observed an increase in RGC survival and no significant change in their electrophysiological properties. When analyzed for alignment, 81% of RGCs were observed to project axons radially along the scaffold fibers, with no difference in alignment compared to the nerve fiber layer of retinal explants. When transplanted onto retinal explants, RGCs on ES scaffolds followed the radial pattern of the host retinal nerve fibers, whereas RGCs transplanted directly grew axons in a random pattern. Thus, the use of this scaffold as a cell delivery device represents a significant step towards the use of cell transplant therapies for the treatment of glaucoma and other retinal degenerative diseases.
The neural retina, like other parts of the mammalian central nervous system (CNS), shows little reparative capacity. Retinal degenerations such as retinitis pigmentosa and macular degeneration in the back of the retina, and glaucoma in the front of the retina, often end with the death of retinal neurons such as rod and cone photoreceptors in the former, or retinal ganglion cells (RGCs) in the latter. In approaches to replace lost cells in the posterior retina, subretinally injected photoreceptors and retinal progenitor cells migrate into the correct lamina of the retina, form local synaptic connections and thereby restore some functionality in animal models.[1, 2] Such an approach for RGC replacement is considerably more difficult, however, given the challenges of local and distal wiring faced by these neurons. Recent steps forward in enhancing RGC migration into the retina after intravitreal cell delivery,[3, 4] sending local dendrites into the inner plexiform layer (IPL),[4] elongating axons into the optic nerve head,[5] and regenerating axons long distances in the injured optic nerve, to the optic chiasm and finally the brain,[6–8] suggest that a transplantation therapy may yet be possible. However, transplanted cells have been unable to direct their axons radially towards the optic disk, perhaps due to the developmental changes in retinal guidance molecules,[9, 10] motivating tissue engineering approaches to mimic retinal neurite patterning. Here we address the radial axon guidance through a newly charactarized electrospun scaffold.
Poly-D, L-lactic acid (PLA, Purac Biomaterials Inc., PDL20) was dissolved in 1,1,1,3,3,3-hexafluoro isopropanol (HFIP, Chem-Impex International Inc.) to a concentration of 6.6 % (wt/vol). PLA solution was pumped at a continuous feed rate via NE-500 syringe pump (New Era Pump Systems Inc.) and ionized in a 20 gauge blunt tipped needle (Hamilton) by a high voltage power supply (SpellmanHV, 230-30R). A radial collector was constructed with a 1 mm diameter copper wire acting as the central pin and a plastic cup, 1.8 cm diameter, coated around the outside and upper rim with aluminum foil mounted on the central pin, with both the central pin and cup connected to the same ground. Flow rates, voltages and void distances between the needle and the radial collector were varied to create scaffolds with different properties (Figure 1f–g).
Scaffolds were characterized for fiber diameter and fiber alignment by scanning electron microscopy (SEM). Samples were sputter coated with gold and imaged at 500× and 5000× magnification under high vacuum using a FEI XL-30 Field Emission Environmental SEM. 15 fiber diameters were measured from 3 regions of interest of 3 scaffolds at each flow rate, voltage and tip-to-collector (evaporation) distance using the measure tool in ImageJ (NIH). Fiber coherency was analyzed on SEM images using the OrientationJ plugin (Biomedical Image Group, Ecole Polytechnique Federale De Lausanne) for ImageJ.[11]
Retinal ganglion cells (RGCs) were purified to >99% by sequential immuno-panning as described previously.[12–14] Briefly, retinas were isolated from early postnatal rat or GFP positive mouse (Jackson Laboratory) litters (postnatal day 2–5), digested using papain and dissociated to single cells using mechanical trituration. After a negative selection to remove macrophages and endothelial cells, RGCs were isolated by Thy1 reactivity. RGCs were cultured at 37°C, 10% CO2 and 100% humidity in Neurobasal media supplemented with insulin, sodium pyruvate, penicillin/streptomycin, n-acetyl cysteine, triiodo-thyronine, forskolin, Sato, B27 and BDNF and CNTF growth factors at previously published concentrations.[12–14]
PLA scaffolds were cut from radial collectors and sterilized for 20 minutes by UV irradiation. Following sterilization, scaffolds were equilibrated to culture conditions by incubating with laminin (2 μg/ml, Trevigen) in Neurobasal medium (Invitrogen). Control tissue culture plates were coated with poly-D-lysine (PDL, 1mM, Sigma) for 30 minutes, washed and coated with laminin overnight. All samples were seeded with 35,000 RGCs.
RGCs seeded on PLA scaffolds were incubated with calcein AM (1:500 of 1 mg/ml dissolved in DMSO, Invitrogen) and propidium iodide (1:500 of 1 mg/ml, Invitrogen) in culture media for 30 minutes at 37°C and 10% CO2. Following incubation, fresh culture medium was added. Samples were imaged using an Axio Observer Z1 inverted fluorescent microscope (Zeiss). Measured values for each culture condition were evaluated for significance using an unpaired student t-test.
Whole cell patch clamp recordings were performed on neurons cultured on PLA scaffolds and control tissue culture plates using standard wall borosilicate glass patch pipettes (Warner Instruments) with tip resistances between 4–6 Mohm. The external bath solution contained (in mM) NaCl 140, CaCl2 2, MgCl2 1, HEPES 5, dextrose 3, and the pipette solution contained (in mM) potassium gluconate 100, CaCl2 5, EGTA 10, and HEPES 10. Current clamp recordings were made using an Axopatch 200B amplifier (Molecular Devices), filtered at 10 kHz with a low pass filter (Ithaco), and digitized at 5 kHz (Digidata, Molecular Devices). Pipette resistance was compensated by adjusting pipette offset. After break-in, the cells were held at −60 mV and stimulated with 200 ms current pulses. Traces were analyzed using Clampfit (Molecular Devices). Measured values for each culture condition were evaluated for significance using an unpaired student t-test.
Samples were fixed for 30 minutes with 4% paraformaldehyde (Electron Microscopy Sciences) diluted in phosphate buffered saline (PBS, pH 7.2). Samples were washed 3× with fresh PBS and then blocked for 30 minutes with 10% goat serum (Invitrogen) with 0.2% triton X-100 (EMD Millipore) to permeabilize the cell membrane. RGCs were stained for βIII tubulin (mAB, 1:500, Covance) overnight at 4°C, washed 3× with PBS and labeled with Alexafluor 488 goat anti-mouse IgG (1:500, Invitrogen). Samples were imaged using a TCS SP5 Inverted Confocal Microscope (Leica) with mosaic images stitched using LAF software (Leica).
Imaged scaffolds were divided into 4 sections and all neurites greater than 2 cell bodies in length manually counted for radial alignment. The percentage of aligned neurites in each section was averaged to produce the cellular alignment for the scaffold. Finally, the cellular alignment for 3 scaffolds, produced on separate days and seeded with RGCs from separate cell isolations were averaged to give radial scaffold cellular alignment, reported with standard error.
Cellular alignment was calculated on RGCs seeded on scaffolds, control tissue culture plates and retinal explants. Regions of interest (ROI, n≥6) were chosen at random, excluding scaffold areas with deformations or holes, and the angle from the center of the scaffold, optic nerve head or center of image for control plates to the center of the ROI was measured. Cellular alignment was calculated using the distribution function of the OrientationJ plugin, which listed the percentage of neurites aligned on each degree from normal. Re-centering the data to the angle measured from the center point, the percent of cells aligned within each 10° from the true radial angle in both directions was summed for each 10°from 0° through 90°. Cellular alignment for RGCs cultured on scaffolds, control plates and retinal explants for each 10° bin was analyzed by one way anova statistical analysis coupled with a post hoc t-test for significance.
Whole rat retinas were dissected from adult Sprague-Dawley rats, flat mounted on membrane culture inserts and cultured in RGC culture media as described above. 30,000 RGCs isolated from GFP mice were seeded in 50 μl either directly onto the explanted retina or onto a laminin-coated radial scaffold. Cells seeded directly on explanted retinas were cultured for 5 days and then fixed as described above. Cells seeded on ES scaffolds were cultured for 3 days and then placed cell-seeded side down onto the flat-mounted retinal explant, with application of 50 μl matrigel (BD Biosciences) between. Explants with scaffolds were cultured for an additional 3 days and fixed. Explants with and without scaffolds were immunostained for GFP (1:200, Invitrogen) and β3 tubulin (1:500) as described above.
To guide RGC axons, electrospinning was used to create a scaffold from the biodegradable polymer polylactic acid (PLA) using a collector containing a central pole surrounded a ring grounded to the same point (Fig. 1a) to create a 1.2 cm diameter scaffold containing radially oriented fibers with a random fiber orientation over the central pole (Fig 1b–d). [15] Scaffolds produced at varying voltages, feed rates and collecting distances created scaffolds with different mean fiber diameters (Fig 1e) and directional coherencies (Fig 1f). The alignment of the spun fibers increased with the voltage applied. However, scaffolds created at the higher voltage of 20 kV were thinner due to nonspecific grounding during the electrospinning process, which produced too great a pore size to support retinal ganglion cell (RGC) seeding (Fig 1g), while scaffolds produced at the lower voltage of 12 kV included non-uniform, beaded fibers (Fig 1h). Scaffolds produced at 15 kV with a flowrate of 2 ml/hour and a collecting distance of 12 cm yielded the most uniform coherency (Fig 1i) and were used in biological studies.
We next assessed cell viability of RGCs on these PLA scaffolds, which were sterilized and coated with laminin to enhance RGC adhesion during seeding. After 2 days in culture, purified RGCs were analyzed by calcein-AM and propidium iodide staining, to label viable cells and those with disrupted membranes, respectively. When compared to RGCs cultured on polystyrene tissue culture plates coated with poly-D-lysine and laminin (Fig 2a), RGCs seeded on PLA scaffolds (Fig 2b) showed a 50% increased survival (Fig 2c). Thus although the extracellular matrix ligand is the same in both conditions, RGC survival was higher on the fiber scaffold than in traditional 2-dimensional culture.
Having demonstrated high RGC survival, we next asked whether RGCs cultured on radial PLA scaffolds retained their functional electrophysiological properties. RGCs were analyzed by whole cell patch clamping to study their electrophysiological properties. RGCs were purified from age-matched postnatal litters and seeded on tissue culture plates coated with PDL-laminin and on PLA scaffolds coated with laminin, recording from RGCs with standard rounded morphology and branched neurites (Fig 3a–b). Individual cells from both conditions were patched and their electrical properties assessed by current clamp with increasing pulse amplitudes of 200 msec duration, ranging to suprathreshold (Fig 3c). Various electrophysiological properties including input resistance, capacitance and action potential (AP) threshold potential were measured or calculated. AP characteristics including thresholds, peaks, half-widths and time-to-peak were also compared. Measured values were similar between RGCs cultured in tissue culture plates (n=8) and on PLA radial scaffolds (n=5), with no values being significantly different at the p<0.01 level (Fig 3d). Thus RGCs retain their usual electrophysiological properties on scaffolds, consistent with their normal axon morphology.
With high RGC survival and retention of electrical properties when cultured on the radial PLA scaffold, we next asked whether the radial scaffold could direct axon growth of the seeded RGCs to mimic the extension of RGC axons in the retina, which in rodents extend in radial fashion to the optic nerve head (Fig 4a). Laminin-coated scaffolds cultured with purified RGCs were fixed and stained for neuronal β3 tubulin (Fig 4b, ,3b).3b). Measuring of axon patterning across whole scaffolds demonstrated 81.1% ± 2.8 of cells following radial alignment (see Methods), similar to that seen in whole retinal explants. The greater axon alignment when compared to the coherence measurement of the scaffold fibers (Fig 1e) was most likely a function of the fibers spun on innermost surface to the collector having a higher degree of alignment than those spun later, combined with the low RGC infiltration into the electrospun scaffolds.[16, 17] While this low infiltration rate is considered a limitation of the scaffold when used in other tissues, RGCs exist in a single cell layer in the retina, allowing this scaffold to mimic that organization. Using the OrientationJ plugin for ImageJ, we compared RGC axon alignment between retinal explants, 2D cell culture plates, and radial scaffolds. RGCs cultured on the radial scaffold were not statistically different from retinal explants at all orientations away from radial, whereas axon growth was statistically different from the random growth seen on tissue culture plates (Fig 4c, p < 0.005).Interestingly, axon elongation ended at the high density random PLA fibers found at the scaffold center (Fig 1e), and RGCs seeded on this random fiber zone extended only short processes, less than 110 μm, or no processes at all (Fig 4d–e), compared to several millimeters for RGCs on the peripheral radial fibers. At higher density, RGC axons fasciculated into axon bundles which still followed radial directionality (Fig 4f). Thus, key properties of RGC axon patterning and organization seen in vivo were retained on the radial scaffold.
Having established that RGCs axons could be oriented on the radial scaffold in culture, we asked if these scaffolds could be used to direct RGC axons to follow the radial pattern of a living retina in a transplant model. RGCs were isolated from early postnatal GFP transgenic mice[18] cultured in vitro on laminin-coated PLA scaffolds for 3 days as above. Then, scaffolds were placed cell side down onto an explanted whole rat retina. A tight scaffold-retinal interface was maintained using the self-aggregating extracellular matrix matrigel. Separately, GFP-positive RGCs without a radial scaffold were seeded on retinal explants. Following culture for 3–5 days, all samples were fixed, stained for GFP and β3-tubulin, and analyzed by confocal imaging for directionality and integration. When seeded directly onto retinal explants, RGCs did not follow the alignment of the axon bundles of the host retina (Fig 5a). In contrast, RGCs seeded onto radial scaffolds and then transplanted did extend axons along the same radial pattern as the axon bundles of the host retina (Fig 5b). Thus, electrospun scaffolds can deliver properly aligned RGC axons to host retinas.
The loss of RGCs caused by retinal degenerative diseases may be addressed by cellular transplantation to replace lost function, however a major challenge for transplanted cells is to direct their axons towards the optic nerve head of the retina. Electrospinning has been used widely technique for creating scaffolds for the tissue engineering of several different tissues including cardiac [19], vascular [20], muscle [21], bone [22], cartilage [23] and neural [24] tissues. Previously, scaffolds capable of aligning axons linearly into tracts have been created for the central and peripheral nervous systems,[25–29] but more complicated axon patterning, such as the radial patterning required for the retina, has not been demonstrated. Using a collector containing a central pole surrounded by a ring grounded to the same point (Fig 1a), the fibers during the spinning process proceeded first to the central grounding pole and were then moved by the normal whipping motion associated with the evaporation of solvent solution radially between the outer grounding ring and the central pole.[15, 30] Interestingly, when RGCs were seeded on these scaffolds, their alignment was greater than the measured alignment of scaffold fibers (Fig 1f). We hypothesize that this discrepancy in alignment is due to fibers on the innermost portion of the scaffold being more radial than fibers in the middle and outer portions of the scaffold. This decrease in the radial alignment of fibers would be caused by fibers which do not return to the central pole during spinning due to the increased fiber buildup and decreased grounding potential when compared to the lightly covered areas of the surrounding collecting ring. Because the RGCs are seeded on the innermost portion of the scaffold, this increases the probability that their axons follow these more radial fibers.
RGCs must maintain their phenotype and function when cultured. On these ES scaffolds, RGCs demonstrated increased survival compared to those on control substrates. Survival of these and other CNS neurons requires multiple molecular signals including peptide trophic factors, small molecules, and substrate interactions[31]; the possibility that specific scaffold fiber topology enhances viability has also been considered.[32] RGCs seeded on these scaffolds also maintained their electrophysiological properties, capable of firing multiple action potentials in response to current injection. This electrophysiological activity suggests that the scaffolded neurons will be capable of relaying signals once they form synaptic connections to targets in the IPL, although this remains to be tested.
When RGC-seeded scaffolds were compared to explanted rat retinas, RGC axons on scaffolds followed a radial alignment similar to the axons in retinal explants, significantly more aligned than the random directions of RGC axons on control, two-dimensional cell culture substrates. This axon alignment was more evident when RGCs were transplanted to retinal explants either through direct injection or via the ES scaffold. On explants which received a direct transplant of GFP-positive RGCs, transplanted RGC axons did not follow the host RGC axon tracts. In contrast, RGCs on scaffolds aligned with axon bundles of host RGCs after transplant. Although the transplanted axons remained attached to the ES scaffolds, the cell bodies of the transplanted RGCs rested directly on the inner limiting membrane of the retina. This may facilitate dendritic ingrowth towards their synaptic partners in the inner plexiform layer, and will be important to examine in future studies. In addition to their radial alignment, RGC axons also fasciculated into bundles when approaching the center of the scaffold and achieving higher density. The process of fasciculation and formation of axon bundles in vivo aids in organization and axon guidance.[33] In addition, on the scaffold, fasciculation may also provide mechanical support for neurites, preventing damage that could occur during transplantation.
This ES process also formed a random fiber region over the central pole which was observed to stop axon growth of RGCs seeded on the scaffolds. The ability of the scaffolds to stop axon growth at a specific point allows for the alignment of scaffold over optic nerve head where RGC axons in vivo turn to reach targets in the brain. We have observed that RGC neurites can extend away from the scaffold fibers through matrigel, however it is still unknown whether axons would then enter the optic nerve head once implanted following the natural guidance seen in vivo. The optic nerve secretes guidance factors, such as netrin-1, which guide axons to the optic nerve during development and which may aid in this guidance to the optic nerve from the scaffold.[34, 35]
One area where the scaffold does not match the organization of the human retina is in the area of the fovea. In mammals such as rodents, RGC axons project radially directly to the optic nerve (fig 4a), however in humans RGC axons from the temporal retina curve around the fovea as they are guided to the optic nerve. It may be possible to create this effect in future scaffold designs.
While we have shown ex vivo transplantation of scaffolds here, there are several areas which still must be developed before this technique for cell delivery can be translated in vivo, including surgical delivery of the scaffold and attachment of the scaffold on the surface of the retina. Ensuring characteristics such as appropriate elasticity may allow for use in a catheter-like implantation system, similar to that currently used for the insertion of foldable intraocular lenses during cataract surgery[36] and to cell-seeded scaffolds delivered to the subretinal space[37]. The physical attachment of these scaffolds may also require optimization. In these experiments, we used the self-aggregating hydrogel matrigel which transitions from a liquid to a solid at physiological temperature.[38] However, the laminin content found in matrigel may prevent dendritic projections through the hydrogel, as laminin is predominantly found in the ganglion cell layer of the retina and not in the inner plexiform layer into which RGC dendrites project. It will also be important to study the ability of different scaffold constructions to transmit light without significant light scattering, a critical consideration for retinal tissue engineering [39]
We have created a biodegradable electrospun scaffold that mimics the radial axon paths of the nerve fiber layer of the rodent retina. Moreover, our scaffolds increased RGC survival over that of dissociated cultured cells, while allowing the RGCs to retain their electrophysiological properties. Combining these advantages, the scaffolds studied here allowed the majority of RGCs to extend processes along the fibers radially, fasciculating at higher density towards the center, and then stopping at the center point of the scaffold corresponding to the optic nerve head. Finally, by using this scaffold as a cell delivery device, the scaffold was able to orient the axon growth of RGCs to align with axon bundles in the host retina. Together these data suggest that this scaffold may be an important step in RGC transplantation for glaucoma or other optic neuropathies. Combining such tissue engineering with biological approaches that increase axon regeneration through the optic nerve and treatments to facilitate dendritic integration may ultimately address both growth and guidance of the transplanted cells to recreate normal long-distance patterning in the CNS.
We gratefully acknowledge support from the NEI (RC1-EY020297 JLG), NIH center grant P30 EY014801 and an unrestricted grant to the University of Miami from Research to Prevent Blindness, Inc. JLG is the Walter G. Ross Distinguished Chair in Ophthalmic Research. We would like to acknowledge Gabe Gaidosh and the Bascom Palmer Imaging Core for assistance with confocal microscopy. We would like to thank Purac Biomaterials for their generous donation of medical grade biomaterials.
Author Contributions KEK designed and developed the radial scaffold, designed, conducted and analyzed experiments for material, cellular, and transplantation studies, and wrote the paper.
RBM helped design and develop the radial collector and aided in SEM imaging and fiber analysis methods.
PV designed, conducted and analyzed electrophysiology experiments and aided in the writing of those sections of the paper.
JH contributed to the initial conception of the project, developed methods for direct cellular transplantation (Fig. 5A), aided in cellular experiments and aided in the editing of the paper.
ANZ aided in the collection of data for Fig. 1, Fig. 2 and Fig. 4.
DAV aided in the development of a method for quantifying cellular alignment (Fig. 4C) and aided in the collection of data for that figure.
MSU aided in the collection of data for Figs. 1 and and44.
EBL contributed to the initial conception of the scaffold project.
KJM aided in and oversaw the electrophysiology experiments.
FMA contributed to the development of the radial collector and scaffold production.
JLG developed the project, aided in and oversaw all experiments and their analysis. He participated in the writing and editing of this paper.
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Fighting Irish fall to Spartans
Jason Teague's rushing touchdown in overtime Saturday gave the Michigan State Spartans a 44-41 upset win over the No. 10 Notre Dame Fighting Irish.
The Irish had come into this game as a 6-point favorite. The combined score went well OVER the day's posted total (56).
Spartans QB Drew Stanton went 16-of-27 for 328 yards, tossing three TD strikes, running for a score, and getting picked off once. Matt Trannon (with two) and Kellen Davis had TD catches for Michigan State, and Sir Darean Adams returned an interception for a score.
For the Irish, Brady Quinn completed 33-of-60 pass attempts for 486 yards. Quinn threw TD strikes to Jeff Samardzija (three), Darius Walker, and Maurice Stovall in the loss, and was intercepted once.


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Tarot Readings for You for January 6, 2013 Sunday(c)
Guidance Our story today is about doing whatever you are doing, your routines, be okay with it, persisting is what makes you a winner, what makes you victorious. Working for a living, even if you are plodding, makes you a champion of sorts.
Are YOU here for the first time?Come in! People love this place! Full instructions are at the bottom.
Guidance and the Tarot Readingsare for you if you just want your daily look ahead. "Guidance" is the summary, followed by your word for word Tarot Readings from the four Rider Waite Tarot cards you see pictured here. Pick the sentences that you feel are yours and decide whether to encourage or reject them. Sentences contradict one another because negative cards, in this group reading with no question, can attach to any of the other three cards.
Want more? – keep going to Learn Tarot by Observing, and Learn Tarot by Pictures.
Want even more? – call Emily at 800 993-6912 to get yourself a detailed accurate live Tarot Reading, no information from you, or choose an email reading. There's a tab for that on this page. You support 500 acres of animal sanctuary in the wilderness where Emily lives.
The Community of Users in the Comment section, which is the very last thing, would *love* to hear from you.
Ace of Swords
King of Cups – Eight of Pentacles – Three of Wands
Tell the visitors here something useful and meaningful to them.
Headings [So you can choose which belongs to you]
Tarot Readings: A Good Man (King of Cups)
Tarot Readings: Working for a Living (Eight of Pentacles + Three of Wands)
Miscellaneous Tarot Readings
Tarot Readings: A Good Man (King of Cups)
He must be a decent man, he works for a living.
He is the world champion sweetheart, always doing something.
I am absolutely going to be keeping this man in my future.
He works at being the best man he can possibly be.
Doing what he is doing, he will always be feeling absolutely mellow.
At your job, for sure, the man who is your guy will come.
You will be working with the most calm man that ever was.
I will still do this routine to make myself be patient.
Tarot Readings: Working for a Living (Eight of Pentacles + Three of Wands)
If you are going to be working here, you have to be a quiet polite man.
You have to work to make money, be nice about it.
No matter what your weak point is, you work and you have an income.
(He is/I am) going to make myself be calm at work.
You have to keep working for the government.
I am going to keep being calm and polite if it kills me.
You will have to drink as long as you work here.
In this job, you will have to be mild-mannered.
He is a good man, he will get a job for sure.
I/He will have to work in spite of my weakness.
This gentleman will have to work.
Miscellaneous Tarot Readings
Government mandates how each thing is to be done.
Be patient, keep at it, and you will win.
Just persisting will make an ordinary person a winner.
Just keep patiently at the routine you are doing, and you will be a winner.
So emotional you are forced to take it one step at a time for a while.
I will have to keep doing the same thing over and over, and be quiet about it.
Ace of Swords
King of Cups – Eight of Pentacles – Three of Wands
Eight of Pentacles is about work. Three of Wands is money coming in. So the two of them translate 'work for a living.'
Eight of Pentacles is to keep doing the same thing, and Three of Wands means 'for some time in the future,' so the two of them often translate 'will be doing.'
Ace of Swords is a mandate, and is the winner or the victory. It often translates 'have to' or 'must.' It is also the champion (the laurel leaves). Imperative.
Then here is King of Cups back again. He is the quiet patient man, the mellow man, the decent good man, the gentleman, and even the drinking man.
So our story today is about doing whatever you are doing, your routines, be okay with it, this is what makes you a winner, what makes you victorious. Working for a living, even if you are plodding, makes you a champion of sorts.
King of Cups is here as the ordinary no-frills man, the quiet patient man, the mellow man, the decent good man, the gentleman, and even the drinking man. He is emotional: That is his main ingredient. He is a sweet guy (your honey). The water around where he is sitting represents his emotionality.
Eight of Pentacles is about work. Eight of Pentacles is to keep doing the same thing, whatever you are doing, your routines, be okay with that. You see the carpenter at the work bench hammering away at yet another identical item.
Three of Wands is money coming in. Three of Wands means 'for some time in the future' so it can put a nearby card into future tense. It is also about waiting for that money, or anything else, to come to you. It also indicates a continuing process into the future: now and for some time to come. You see a well-dressed merchant eyeballing his ships coming in, waiting for them.
Ace of Swords is a mandate, what makes you a winner, and is the winner or the victory. It often translates 'have to' or 'must.' It is also the champion (the laurel leaves). Imperative. The commander. Something you gotta …. A sword is held aloft, and the crown atop it is garlanded with laurel, representing the champion, the winner.
I N S T R U C T I O N S & Explanations (Comments are below this.)
Did you come here just for your predictions?– If so, you are done when you finish reading the sentences of Group Analytical Tarot Cards Reading.
Each Tarot sentence is a literal-interpretation reading of the scenes pictured on thefour cards you see. Only what each of the cards says – phrase by phrase – no fillers – no psychic ability used. The readings are about you and the people around you. They are about today and the days around it. Tarot, all by itself, talks to you. This is a demonstration of that .
Tarot will address any group flexibly, as it does here of 'all the visitors.' (It can address 'all the people at work who have a say in my raise,'for instance, without you knowing who or where those people are.)
Horoscopes are group readings too.
Astrology has to be based on someone else's birth data, though, so it does not solidly apply to you just because of where the sun was when you were born. (Astrologers know this.)
Emily wants to know whether these sentences are more accurate for regular visitors than for people who have come only a few times. Would you help with a comment, if that is who you are? Please?
Without a questionto focus the answer, four cards can mean contradictory things, especially when the Spread has a card that means 'no, never or not.' That negative modifier can apply to any one of the cards.
Without a questionalso the pronouns (you, him, her, me, us, them, they, etc.) in a group reading are more flexible, especially in some of these Rider Waite Tarot spreads. When a pronoun is in parentheses, feel veryfree to substitute. Notice how, as much as possible, we drop pronouns, which makes for some awkward phrasing.
The question in a 'real' individual reading supplies at least half the information! In a 'real' reading, the parties are identified (including the gender of the questioner!), and so is the time frame if there is one. In this group reading, we cannot do that, so we have variety.
Do you want to know a little, or a lot, about how this Tarot Verbatim ™ thing works? If so,
*Go to Learn Tarot by Observing. This tells the thinking regarding the combinedfour pictures in the Daily Spread – the pictures above here. It explains how these four cards get together in your mind to make the message.
*Go to Learn Tarot by the Pictures.This is the meaning of each separate card. This is how that illustration makes that card's meanings in this Spread.
You can put all this together and intuitively see yourself how Tarot Verbatim ™ works. Regular visitors actually learn to read Tarot without trying. The Tarot Talks tab has some examples for students.
P. S. Call Emily for your personal live reading at 800 99 3 6 9 12 (from Eastern 1 p. m. to Eastern 1 a. m. at least, every day). Emilyanalyzes relationships in business situations and personal situations accurately from *Tarot, *intuitive, and *practical perspectives. She tells you what is happening and what to do about it to make it happen best. (Testimonials about this are on the web site http://EmilysInsight.comand here under its Tab, and in Comments.)
Illustrations from the Rider-Waite Tarot Deck®, known also as the Rider Tarot and the Waite Tarot, reproduced by permission of U.S. Games Systems, Inc., Stamford, CT 06902 USA. Copyright ©1971 by U.S. Games Systems, Inc. Further reproduction prohibited. The Rider-Waite Tarot Deck® is a registered trademark of U.S. Games Systems, Inc.
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In 2015, the state championships for swimming and diving will debut.
Until then, the best of the best in the vicinity can only showcase their talents at the Central Coast Section championships — with one exception, the ninth annual Section Challenge hosted by Palo Alto.
"I think this will actually help even more next year because now people will be able to work on swimming against the best and getting themselves not just ready for their section or their league anymore, but they're getting ready for state," Paly coach Danny Dye said. "So swimmers will get a feel for each and when they get to the state meet, there will be a little bit of a rapport or a rivalry or whatever you want to call it."
Eleven teams from three sections — the Central Coast, North Coast and Sac-Joaquin — will be in the pool today starting at 9 a.m.
But the action began Friday with the 1-meter diving portion of the meet, which saw Paly junior Scott Hillen win the boys competition by scoring 398 points — two shy of his season-long goal.
"For the first couple of dives I was just getting the kinks out," Hillen said. "Then I got into my flow and I kind of really just locked in and did it. But I really wanted the 400 because it's a big goal for me."
Missing for Paly were sophomores Reed Merritt and Mimi Lin, fourth and second at CCS last year, respectively. They were both away at a national meet.
Hillen credited Merritt for helping him improve technically.
"I look at his form and I look at how he does his dives, and I really try to model what I do and make it similar to his," Hillen said. "I know I can't be exactly like him, I have my own style, but the basic of how you throw and how you tuck is all the same."
Hillen wants to crack the top five at CCS, which is why he enjoys the dives with the higher level of difficulty.
"I like the ones that are showy," Hillen said. "The ones that have a lot of turns and twists and flips."
His most memorable of 11 dives at the Section Challenge was an inward 1½ tuck that barely left a splash.
"It went straight in," Hillen said.
Monta Vista-Cupertino junior Qianyi Pang won the girls competition with a score of 468.55. The runner-up was Los Altos freshman Riley Fujioka, who became intrigued by the sport at her country club and now trains at Stanford.
Her score of 425.75 would have placed her sixth at CCS last year.
"Every meet there is more and more girls that I compete with regularly that have really outstanding dives, so you always have to be your best," Fujioka said.
The dive that stuck out for her was a reverse 1½.
"I wasn't going to compete it because I'm really nervous about it," Fujioka said. "But I ripped it this meet."
A name to keep an eye out for in today's swimming events is Paly senior Andrew Liang, the reigning CCS champion in the 50-yard freestyle and 100 butterfly — the two events he will swim at the Section Challenge.
Dye is confident every swim will be worth watching, especially since it's a sanctioned meet that can get anyone qualified into their respective section championships.
"The whole concept of this meet is you get the fastest, so all of the events have an opportunity to be exciting," Dye said.
Email Vytas Mazeika at email@example.com; follow him at Twitter.com/dailynewsvytas.


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Greenies Pill Pockets Duck & Pea Allergy Formula for Dogs (6.6 oz)
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Completely conceal the taste & smell of your dog's medicine with a healthy treat. Duck & pea flavored for dogs with food allergies. Designed for capsules.
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Celebrate "America Recycles Day" with this reminder of just how important recycling is to our planet's preservation.Read More
Recycling is Still Relevant
Celebrate "America Recycles Day" with this reminder of just how important recycling is to our planet's preservation. Read More
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With crisp breezes and colorful leaves, it's no surprise that fall is many Americans' favorite season of the year. Read More
What happens when you leave the lights on?
We all know the struggles of the morning rush – you snooze the alarm 5 times and then have to shower, get dressed, eat breakfast and speed out the door in 20 minutes flat. Read More
How to Make Mornings More Energy Efficient
Becoming energy efficient isn't as easy as turning off a light switch – OK, maybe it can be. Read More
Summer Savings While the Kids Are Home
School's out – it's summertime and the kids are home enjoying their time off. Read More
Program Your Thermostat for Energy Savings
Summer is heating up and raising temperatures in your home. Read More
Save Up to $180 a Year with a Programmable Thermostat
Almost half of a homeowner's energy bills go toward heating and cooling the house, according to ENERGY STAR. Read More
Here's What Could Happen When You Unplug for 24 Hours
Ready, set, unplug! Read More
10 Energy-saving Tips for Spring
As the dreary winter days turn into the promise of spring, it's the perfect time to think of how you can prepare your home for the warm months ahead. Read More
Think Small: 3 Ways Minimalist Living Can Reduce Your Carbon Footprint
Last week, I noticed that my social media feed was filled with friends, family and acquaintances recommending the film Minimalism: A Documentary About the Important Things. Read More
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After a long day of work, most people like to come home and relax. Read More
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A contemporary styled doormat in a brown and blue ongoing circle design that ascends in size.More Details >
It reflects a bit of impressionistic art in its chic print and attracts attention. Soft and thick, its wool and silk art fabric construction ensures quality. This contemporary tufted doormat in blue and brown makes a stunning addition to modern decor.


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Jackson State Community College dance program
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Information about Jackson State Community College dance program, and registering for discussion sections and departmental seminars. As a student, your first stop should be the registrar's office, where you can pick up a free copy of both the academic calendar and course catalog, in order to find available credit classes that you may be interested in taking. In-person class registration has largely been replaced with online sign-up, and many schools offer mobile apps that streamline the course registration process. Finally, check with your major department for assistance with prerequisites, tuition payment deadlines, and Jackson State Community College Choreography information. Please use the form above to request admissions info for Jackson State Community College.
Not all people working as actors in film, television, or theater are professionally trained. For example, Bob Hoskins didn't have any formal training before taking up acting. Universities may offer drama programs derived from the system of Constantine Stanislavski, which was popularized in America by Lee Strasberg and Stella Adler. Regardless of a school's approach, students should study human emotions, character interpretation, voice and dance.
While most modern theatre companies rehearse only one new piece of theatre at a time, and then perform that piece for a set run, retire the piece, repertory companies often rehearse multiple shows at once. Repertory theatre involves a group of accomplished actors, and relies more on the reputation of the group, rather than on an individual star performer. Further, actors who have worked together in several productions can respond to each other's nonverbal cues without relying as much on external direction or adherence to theatrical conventions. Beyond consideration for Jackson State Community College dance program, you may think about Los Angeles Southwest College remedial classes for complementary electives to your primary course of study.
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1. Psalm 19 speaks of the heaven's proclaiming the glory of God - and the sun's part in that is to be like a bridegroom moving through the sky. That's not abstract power, it's LOVE. Look at the sky and see the love of God.This is God's world and it is singing out the gospel. What can you see?
2. The gospel pattern is that the Triune God sends the Son into the world as The Light who shines in the darkness. The darkness tries to overcome the light but cannot. The moon shines at night to hold back the darkness, and the sun does so in the daytime.
3. And, Jesus died in the dark and rose in the morning. The end of the day proclaims the death of Jesus, the new morning proclaims the mercy of God - it proclaims the resurrection.
See also:
Theology for the cold days too
Glen Scrivener: This is The Man's world


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Shoppers Hit the Bricks – and Clicks27 Nov, 2002 By: Holly J. Wagner
Shoppers are hitting malls and keyboards early this year, starting their holiday shopping earlier and doing more of it online, a variety of retail analysts reported.
Perhaps the most telling report is from the U.S. Department of Commerce, which reported a 34.3 percent increase in online shopping for the third quarter of this year from last year, and a 7.8 percent increase from the second quarter of this year.
It's no wonder, taking into account the $1.5 billion in online spending that Web site traffic measurement firm comScore Networks logged in the week ending Nov. 17 -- a 28 percent hike from the same week last year.
"E-commerce will continue to outgrow traditional retail, as the Internet appeals to growing numbers of consumers in search of the best deals, convenience and breadth of offerings," said comScore VP Michelle David Adams. "At the same time, continuing fluctuations in this growth offer a valuable leading indicator of this fragile consumer economy."
That report held good news for the home video sellthrough industry, as comScore found a whopping 92 percent increase in spending on consumer electronics and a 68 percent increase in movie and video sales online for the week ended Nov. 10. By comparison, online toy spending was up 37 percent and clothing purchases were up 70 percent.
Not surprisingly, Amazon.com was the top online seller in the comScore survey. Also in the top 25 were Columbiahouse.com at No. 6, CDNow.com at No. 7, BestBuy.com at No. 13 and online rentailer Netflix.com at No. 20 -- a more than respectable showing against online sellers.
Whether the online spending trend can continue into next year will likely hinge on how long e-commerce ventures can operate without paying sales tax. Earlier this month, 30 states signed onto a proposal that outlines the basic structure for an Internet sales tax.
Retailers reporting to Shop.org, the online division of the National Retail Federation, confirmed a shopping surge early in November, with 60 percent posting revenue increases of 25 percent or greater in the first two weeks of the month, compared to the same period last year.
Free shipping with conditions has been the most successful tool in driving holiday business, 47 percent of e-tailers reported. Search engine placement, best-seller lists and early shopper discounts have also been effective.
"The 2002 online holiday season is off to a great start," said Scott Silverman, executive director of Shop.org. "We're encouraged to see retailers reporting online sales increases during these early weeks and expect this trend to continue."
It remains to be seen whether that will translate into increased spending for the year. Projections for holiday spending range from flat to modest growth, with the most ambitious estimates at 5 percent to 6 percent growth.
Findings last week from analyst firm The Conference Board indicate consumers will spend 5 percent more on the holidays this year than last. "Despite widespread reports that worried consumers will sharply curtail their holiday spending, the board's annual survey projects a 5 percent increase in Christmas spending this year," said Lynn Franco, director of The Conference Board's Consumer Research Center. "Continued discounting by retailers, both online and in the stores, is likely to prop up Christmas spending."
Low entry-point pricing is helping consumer electronics and software to a sales surge this year, but computer makers are unlikely to feel the same benefit.
Gartner Dataquest predicts just a 1.5 percent increase in PC sales, even though many are equipped with media center features this year that weren't widely available last year. The PC industry's loss may be the home entertainment industry's gain.
"The gloomy economic situation is likely to affect U.S. and Japan holiday season purchases," said George Shiffler, principal analyst for Gartner Dataquest's computing platforms and economics research. "Given that home PC penetration is relatively high in the world's developed economies, limited funds may induce users to extend the life of their PCs. Many families may also choose other devices such as game consoles, DVD players and digital cameras instead of upgrading their old PCs."


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CT Deanery Unction Service 2011 - 04/10/11Gathering at Three Saints Church, Ansonia, CT the clergy and faithful of the Connecticut Deanery celebrate the sacrament of Holy Unction with our Archpastor, Bishop NIKON.
(70 images)
Service of Unction - 03/29/15
The Service of Unction was held at Three Saints Orthodox Church in Ansonia, CT
(17 images)
Episcopal Visits - 09/28/15
The pictures present in this gallery are just a few from the visits of His Eminence Nikon to the parishes of Berlin, NH (celebrating their 100th anniversary), Springfield, VT and New Haven, CT (also celebrating their 100th anniversary).
(35 images)


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White House press secretary Jay Carney said Wednesday that passing a comprehensive immigration reform bill has "always been an uphill battle."
Still, he acknowledged, "Hard things are hard."
The White House spokesman would not outline Obama's plans for pushing the legislation in the House and would not detail whether the president would stay on the sidelines as he did as the Senate considered the legislation or if he would launch a more public campaign.
"The thing about strategy is it would be strategic foolish to announce it in advance. We are on this every day," Carney said.
But Carney said immigration has gotten this far "in no small part due to the president's advocacy."
Obama met with members of the Congressional Hispanic Caucus on Wednesday to discuss an immigration reform bill. But lawmakers and senior aides have expressed skepticism that a bill could pass the House.
"In the end, we can't prevent lawmakers from making bad choices. We can simply be part of a broad, comprehensive effort of persuasion," Carney said.


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A recent letter to the editor expressed disappointment at the lack of tickets available during the pre-sale for R.E.M.'s upcoming summer show at Lakewood in Atlanta ("Web sites should do better job with tickets," Saturday). As the R.E.M. employee most closely involved with these sales, I felt it might be of some use to clarify the nature of the sale.
Ticketmaster allows bands to sell a very limited percentage of the overall tickets at any given show, including some of the best seats available with reduced "convenience" fees, to fan club members before tickets go on sale to the public. We're always careful to state that tickets are limited and are sold on a first-come, first-served basis. In the case of the Atlanta show, sales were brisk, and almost the entire pre-sale allotment available was purchased by registered fan club members on April 1. This left only a few seats for our e-mail list subscribers, whose sale began at 10 a.m. April 2. In a matter of seconds, the remaining share of tickets was gone, leaving the letter writer and others, sadly, without access to reserved seats through the sale. However, it wasn't a matter of the Web site's inability to handle multiple hits, as the writer contended, but rather the fact there were so few tickets left.
I assume the writer didn't see the Thursday Banner-Herald article in which an Athens-only sale was announced. Our office sold a large quantity of tickets to Athens-area fans Thursday, and there were still a few good seats available by day's end.
While we certainly regret the writer's misfortune, I feel we went to great lengths to take care of fans like him by making sure fan club members and local fans had a fair chance before things opened up to the general public. Unfortunately, factors such as limits on tickets and high demand superseded our ability to accommodate everyone in the online sale.
David Bell
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Kukla's Korner Hockey
Intense Gretzky
by Paul on 11/25/05 at 02:08 PM ET
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More than a month into his first season as an N.H.L. coach, Wayne Gretzky said, he is asked most often whether he has the urge to jump over the boards. Gretzky, the greatest offensive player in the history of the game, is coaching one of the league's most offensively challenged teams, the Phoenix Coyotes. "I've really enjoyed each and very day on the bench," Gretzky said recently. "I've enjoyed the practices and the players." Steve Ellman, the team's principal owner, spoke about Gretzky's influence with the team beyond coaching. "Wayne's been magical for our franchise," he said.continued
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About: University Hospital Lewisham University Hospital Lewisham London SE13 6LH
Posted via NHS Choices
Brought mum (aged 87)in for her hearing test doctor was patient and explained every stage of the test and the out comes to her.. Brilliant that we had a Saturday appointment.. Mum is so happy she can take part in family conversations and can watch tv without subtitles.. Thank you so much for improving the daily quality of her life . Many many thanks
Response from University Hospital Lewisham 2 years ago
Thank you so much for feeding back your mother's experiences of our ENT services recently and that she has benefited so much by the care offered. It is always greatly appreciated when patients or their relatives take the time to tell us about the care they have received and I will ensure your comments are shared with the team. Thank you again and I hope your mother continues to enjoy her improved hearing. Sue Ludlow, Patient Experience Manager
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The breakout novel that puts S. Andrew Swann in the technothriller ranks of Clancy, Coonts, and Cussler!
Veteran writer S. Andrew Swann turns his talents to a mainstream technothriller that combines the authentic detail of Tom Clancy, the global intrigue of Jack Higgins, and the sheer adventure of Clive Cussler. Gideon Malcolm was a Washington, D. C., cop who stumbled into the midst of a high-level sting operation gone wrong and nearly paid with his life. Now he wants answers. But the government wants him to forget the whole thing. Yet Gideon wasn't about to give up, and what began with a stolen supercomputer soon led him on the trail of a missing woman, Dr. Zimmerman - and into the heart of a conspiracy so vast, so devastating, that the entire world would be caught up in its power!
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FASTARTUP is a leading digital creative studio in Singapore providing a wide range of solutions, ranging from graphic design, web design and development and branding.
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friend of the devil by micmojoWatch
©2012-2017 micmojo
Beautiful, reminds me of "old" sx70 polaroid.
It is indeed very Edward Hopper. I NEED a print of it. I absolutely love it.
sorry Edward Hopper
fantastic !!!!! like a painting... of Howard Hooper
Well done, reminds me of a traditional painting. Great job.
great lighting, beautiful shot
lovely photo nice colour and lighting
great colors. the light and shadow look beautiful with the greens.


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St. Lucia to Marathon, Florida, was the most beautiful of passages until it wasn't.
Published on May 24th, 2017 | by Moore 24 Class
Twelve hundred miles were full of fine sailing, and the last one hundred full of frustration and despair. The above image of GANNET's Yellowbrick track only suggests the agony of those last miles. I was aiming ... Read More →


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Social Daddy
Everything that modern marketing percieve as a necessary tool is of interest to me.
SM is easy just like...S/M
Kamil Dziadkiewicz
When I first started doing social media marketing i was convinced, that this is going to be the easiest thing ever. I am good with words, not bad with basic and not so basic concepts in web and mobile technology, so I was sure it won't feel like working at all.
And...it can be like that. As a ma...
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Posted Jan 25, 2013


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May 09, 2009
– Comments (7)
Welcome to Reality....from NewsweekKevin KellyNewsweek Web Exclusive
Don't tell me that the economy is getting better, or has even hit rock bottom. My faith in an imminent recovery deserted me on May 5, when one of our customers, Salyer American Foods, based in Monterey, Calif., suddenly fell into receivership. There had been little to no indication that the company was so close to financial ruin. As it turns out, the company's lenders say Salyer owes them over $34 million, a debt equal to almost half its sales. A company attorney told local media that tight credit markets and the economic recession had pushed Salyer over the edge. If the receiver doesn't find some way to revive the company's fortunes, our bag manufacturing company stands to lose nearly $1.5 million in revenue, about 2 percent of our $60 million in sales.
On the same day my customer fell into receivership, Fed chairman Ben Bernanke told a congressional committee that he believed the economy was in the process of bottoming out and "would turn up later this year." He's not alone in his optimism. Over the past two weeks or so, it has become a cottage industry among economists and the media to spot the first "green shoots" of a recovery. Certainly shoots there may be. The stock market has rebounded smartly over the past two months, as has consumer confidence. Pending home sales have ticked up, while unemployment claims are easing. And many economists insist a manufacturing revival is in the wings because inventories have fallen so low that restocking must begin soon.
But I haven't found many small-business owners ready to jump on the recovery bandwagon, and for good reason. We're still experiencing the "bottoming out" phase and worry that another bottom remains below this one. Call us pessimists, but we're not sure the green shoots aren't just weeds.
Who can blame us? Take the experience of a friend of mine, who runs a $6 million company that provides promotional material to businesses. His sales are down 20 percent compared with last year. Over dinner last week, he said he certainly wasn't shedding customers at the same pace he had been in the fall, but customers were still defecting. "I can't see any reason why they'd come back soon," he said. So he's getting ready for a second round of layoffs and plans to end spending on marketing until things look more promising.
He's not alone among my friends and colleagues in his sense that bad times may be here to stay. One friend just decided to abandon her two-year-old Web-based gift boutique thanks to declining sales. She has another friend whose promising e-business startup had its venture funding yanked when it failed to meet sales goals. "Two years ago they'd have been given time to work things out," she says. Instead they recently closed. Another friend of mine works for a commercial real-estate company that's instituting 10 percent wage cuts beginning in mid-May. "It's better than people losing their jobs," he said to me, "but I don't expect to be getting the money back any time soon." Given the growing worries about commercial real estate, he's probably right.
Even some companies that are supposed to be recession-resistant remain worried. I know the general manager of a small candy company, who says his sales haven't stopped sliding despite the belief that people supposedly eat more comfort food during bad economic times. He has cut back a shift and won't be rehiring soon. Representatives for a small local bank have told me that they haven't seen an uptick in business lending, and that they don't have businesses looking for money other than those they wouldn't lend to in the first place. And a long-time machine supplier of mine has had a completed bag-making machine on its floor since late 2008, when the customer decided not to go through with the purchase. Despite a steep discount, the company can't find a buyer.
Based on my company's experience, I don't necessarily see a positive side to low inventories. Over the past several months, we've seen lead times on orders fall at least 30 percent. Where our customers used to give us three to four weeks to fill an order, now they give us as little as two. Shorter lead times have followed the trend toward smaller orders. Where companies would once order 3 million bags and hold them on their floors for several weeks, now they're asking for only 1.5 million and reordering at the last possible moment. In most cases, it's not that their sales are falling, just that they're slashing order sizes and reducing lead times in order to avoid tying up capital in inventory. Since they're entering smaller orders more often, we're less likely to hold inventory as well. In my corner of the manufacturing sector, the revival that economists have been pointing to seems a long way off.
Now, I know businesspeople can be notoriously wrongheaded when it comes to spotting trends. Aren't the Big Three automakers at least partly responsible for their own demise because of their failure to anticipate the need for more fuel-efficient cars? I know I've almost blown the opportunity to capitalize on growth in the past by being too conservative about buying new equipment. In fact, I've angered customers by stretching out lead times rather than investing, because I've been worried that the sales growth isn't sustainable. Talk about a self-fulfilling prophecy. Even right now, when I can see that a judicious equipment purchase could propel our company forward, I worry about taking on more debt and hold back, even with one supplier offering terms that would give us a machine for a year without any payments.
Then I have a day like May 5. I read the Fed chairman's testimony and feel a bit upbeat, only to get surprised by the collapse of Salyer. Certainly, as my brother points out, it is unlikely we'll lose the entire sales volume even if our troubled customer disappears, because other customers will step in to fill the gap, and they'll buy product from us too. But is it insane to hold off on optimism when you're not sure whether another customer could bite the dust? Perhaps waiting to make any big investments makes sense until we're completely sure recovery is on its way. Of course, if I wait, and lots of other businesspeople like me wait, what will become of those green shoots that may be dotting the landscape?
Link to the above article...
The contents of the above is consistent with many of the small business owners and CFOs I am consulting with day after day and week after week.
This chart did not format correctly. You'll appreciate this website, I think.
Please note that the 2004/5/6 period is a function of drastic changes to the bankruptcy code. Basically the bankruptcy contract between the debtor and the creditor changed in 2005 to dramatically benefit the creditor. People filed quickly in 2005 before changes went into effect in 2006.
It is not like bankruptcy laws are all that sacred despite the whining of CNBC.
What few realize is the lifeblood of American jobs is small and midsize business. Most of the mainstream press and media caters to Wall Street.
Right now millions of small and midsize businesses are silently on the verge of filing bankrupctcy and shutting down. This could impact tens of millions of jobs.
It is why Alstry and the Institute are usually about six to twelve months ahead of the widely reported data and why we have a much clearer picture of the employoment situation.
I don't fault the caps players that can't see, they simply don't have the right prescription to see clearly.
Salyer needs to get an "exorbitant" no-bid Pentagon contract!!
In every recession or depression, the recovery cycle has a lag in it. The length of the lag varies based on how deep the recession is, long the recession last, and the downstream effects of suppliers to those industries that are affected. The key indicator for showing the start of the recovery cycle is the market.
When you see the market starting to improve, you see a change in preception in the people that drive the market(investors) and accompaning change in the flow of businesses. The lag is the time required to reach from the heights of the corporate world to the small Mom&Pop business. This lag runs from 3 to 6 months before all sectors are in or starting recovery.
Generally, most recessions are not cause by financials institutions and if there is one weak point in our economy where you don't want issues, it's there. During the recessions of 1893, 1907 and the Great Depression, Banks were the focal point for the start of these events and they begin long periods of recovery. These periods effectively ran for 5 to 10 years before our economy was in full recovey.
In fact, the period before the recovery of the 1893 and 1907 recessions would have run a lot longer if it weren't for the likes of J.P. Morgan and friends putting personal wealth into the economy and the US Treasury Dept. Both of those events precided the existence of the Fed.
During the Depression, a new institution called the Fed tried to do what it could to keep the Depression from occurring but unfortunately they were new to this and made a critical mistake by reducing the money supply which cause the recession to become a depression and having a protracted period of recovery to occur.
You should note that this time, we have the Treasury Dept. printing cash like there is no tomorrow (simular to what J.P. Morgan did) and this causing the money supply to expand. Then adding in the Fed rate at near zero to stimulate new loans. The effect of both should reduce the amount of time needed to recovery the economy.
What we should see and I believe that we are is a shorter period of recovery. Instead of years recovering, it looks like months. But we still have that lag to account for before it works its way down to the Mom&Pop businesses and each sector has its own unique rate of recovery that has to be taken into account.
What this means is that the people at the bottom have just got to wait for the lag to play out and nothing is going to hurry it along any faster than it does. Granted this doesn't help those in bankruptcy or looking forward to that event, but to those that survive this, it is a note for their future. Keep more cash in hand and if it looks like the banks are in trouble, start hording cash before things get any worse.
Because, you can bet the banks will be in trouble again some time in the future.
ico you just don't get it. this is not lag.
We are not really pumping shit into economy. they are pumping money into banks for nothing, just to keep them alive. Yet, the country and taxpayers will have to fork out the interest on that massive amount of money.
And what is worse, not just banks are highly leverage. Most americans are highly leveraged, Each job cuts have great chance lead to a personal bk if they don't find another job in 8 months. More will simply go into foreclosures. In those years Americans were not this much in debt. And in those years, standard of living were never this high and this high burn rate. For each family who lost job need at least 2.5k to keep everything float. It is just impossible to stop this domino action.
Recovery certainly looks a good way off for many small businesses right now. I have recently closed my small retail business seeing in May of last year my costs going up on the bottom side and sales flattening due to the price of fuels and food. We noticed in our gross/average sales and traffic numbers that consumers had already begun cutting back on discretionary spending months before the press began reporting it.
In November I decided to not renew my lease even though the shop was still operating in the black (without dipping into our business cash reserves) and it would feasibly continue to be marginally profitable for at least another year given our projections and the data on hand, banking on no recovery or return to spending by consumers. Given the lack of business lending and confidence in the retail sector I had to change my exit strategy from selling the business to liquidation.
In the past few months I spent some time liquidating my inventory and fixtures (I'd have liked to have done better) into cash and concentrated just getting out before the rest of the teetering dominoes started flooding the market with goods and retail fixtures. Since I announced my closing, four more retail businesses in a similar field (one a region-wide retailer) have announced their closings.
I don't see this turning around very quickly. Most independent businesses are dying deaths caused by a thousand cuts. Higher lease costs, higher utilities, lowered sales both in terms of actual numbers of sales and lower dollar amounts of the sales that get transacted. Many don't have the option of just deciding to close without a bankruptcy or foreclosures looming in their future. Most struggling small businesses continue to struggle. I see any recovery or the shaking out of businesses playing out at least over the next two years or more locally. Interesting conversation.
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text: I was at the Sprint Store today, and I was talking with one of the reps, who I've been going to for a few years now. I got on an alrady 50+ person long waiting list, but, while doing so, she told me why there's such a shortage of the EVO. Below: "The reason the EVO is unavailable everywhere is because of a defect. The defect is something about the screen seperating from the phone, I think. They told me they were redoing it, then mass-producing them." There you have it. Once HTC fixes the light-leakage, we'll have EVOs easily accessible. If this informed you in any way at all, could you please hit that Thanks button? Thanks.


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Os Abutres
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Picture: Katie Price with Peter Andre and their daughter Princess Tiaamii and son Junior doing some shopping on Robertson Boulevard Los Angeles, California - 20.04.08
LATEST: Hospitalised Australian singer Peter Andre is too ill to notice his wife at his bedside, she claims.
Katie 'Jordan' Price, who is heavily pregnant with the couple's second child, has kept a vigil at her husband's bedside at the East Surrey Hospital in Surrey, England since he was struck down with viral meningitis last week (ends28Apr07).
But the visits are taking an emotional toll on the glamour model because Andre is so sick he is often unaware of her presence.
Price says, "He's really poorly. He's usually sleeping, or drifting in and out. Yesterday I went in and he didn't even know I was there.
"I'm checking on him all day, calling the hospital and then every night I take him a towel in so he can smell home."


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acrdl (08-19-2011)
There is no hack for crossfire, why ?
It's more hard to do than crossfire hacks ? Or no1 want to do ?
Don't give a fuck
acrdl (08-19-2011)
The reason is.... crossfire is more popular, it draws in the attention of more coders.
Terell. (08-19-2011)
It's because CF is a lot easier, let me do an example.
For hacking CF you need
A DShell, a base and a detour.
To hack ava you need multiple detours a base + Functions added on it. A good program to encrypt the main source.
Im Jazk
Ske1th (08-19-2011)
Last edited by Terell.; 08-19-2011 at 01:20 PM.
Warrock Minion 8-13-2011 - N/AA.V.A Minion since 11-1-11 - 11-12-11
Thanks all for the awnser
@Jorge.P I know, I'm just saying there won't be any hacks until someone decides to code one. I would but I'm only extremely basic in programming knowledge, I can't code for shit yet.


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A child looks through the window from Ski Dubai to a restaurant of higher temperature...Ski Dubai, an indoor ski resort in Dubai. 22500 square meters of indoor ski area. Part of the Mall of the Emirates, one of the largest shoppingmalls in the world..An extremely efficient insulation system helps the faciity maintain a temperature of -1 °C during the day and -6 °C at night when the snow is produced. Nevertheless, the facility consumes 3000 gallons of oil each day, generating huge amounts of greenhouse gases...Photo by: Eivind H. Natvig/MOMENT
- Copyright
- ©Eivind H. Natvig/MOMENT
- Image Size
- 5616x3744 / 9.2MB
- Contained in galleries
- Dubai by Eivind H Natvig


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BT University
Desigo CC courses offered by BT University focus on monitoring and controlling building automation systems using Desigo CC software at facilities with Siemens Insight brand equipment communicating through a BACnet protocol.
Each instructor-led class uses the latest version of Desigo CC software and building automation simulators with control equipment running on a BACnet protocol. Topics covered in these Desigo CC courses are designed around the unique features and fundamentals of building automation systems with BACnet protocols and the front end software.
BACnet is a data communication protocol for building automation and control networks. This protocol was developed and maintained by the ASHRAE Standing Standard Project Committee 135 (SSPC 135). Consolidated international BACnet standards are updated and published every few years. Learn more about BACnet protocol at www.bacnet.org.
The Desigo CC control center was designed with input from building operators around the world to help improve building performance by integrating a wide range of systems including building automation, fire and life safety, lighting, IP cameras, and power and energy. Desigo CC delivers an integrated approach to enhance and protect building systems by offering new ways to gain optimum control over facility resources today and long into the future.
BT University also offers courses for facilities with building automation systems using proprietary protocols (P2 and TCP/IP) and facilities using Insight software using BACnet protocol.
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Lake Ontario Fishing Report – TSI Charters In Oswego, New York
Captain Joe,
The boys had a great time and commented that each fish was the largest they had ever caught! Your DVD video of our trip is a great memento and is greatly appreciated. We all had a terrific day on the Lake and wanted to commend you for making our trip a great experience.
Andy M.


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"Yes Ma'am"
"For WHAT?"
"Are you new to the state Ma'am?"
"Have you been here over 30 days?"
"Have you been working for more than 30 days?"
"Have you registered your vehicle in the state of Texas?"
"Well, in the state of Texas, it is mandatory that you register your vehicle within 30 days. Your employer has reported earnings for you and the state knows that you are here. I'm sorry but you will have to come down to the station with me."
I looked past the officer and see his police car parked against the curb of my street. It's beginning to dawn on me that he is serious.
Remember that it is fairly late at night and I am in my robe. The officer kindly let me change into some clothes, I grabbed my purse and was escorted to the back seat of the police car. I was scared to death and was SO relieved that he didn't put handcuffs on me.
I couldn't believe that this was happening to me. Here I was a very young 20 year old girl, I was VERY far from home and I wanted my DADDY!!!
I was taken to the Mesquite County Jail. My coat was removed, they took my purse and emptied out my wallet to inventory my belongings. I had 23 cents on me.
They had a lady sergeant come to the front office and made me put my hands on the counter and spread my legs while she frisked me. I was fingerprinted and photographed.
Now it was time to take me to my cell.


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It seems like the babies are starting to feel better. They are finally eating well and sleeping a little better, they are being much more playful and are acting like themselves. It seems like they have been sick all winter. I guess that is the downside of day care. Luckily our neonatologist that comes for home visits and gives them the Synergist shot got them approved for one more shot so they have the RSV protection for the rest of this season. I wish they would be eligible next year but they won't be because they have been too healthy. How can anyone be too healthy?
At the home visit on Thursday we got the babies latest stats:
15 lbs 10 oz
26.5" long
17" head circumference
19 lbs
27.75" long
18" head circumference
13 lbs 3 oz
25" long
16.5" head circumference
16 lbs 4 oz
26.5" long
17.5" head circumference
We are talking about switching a couple of them over to rear facing convertible car seats. I am having a hard time carrying Brady and his seat up and down the stairs to get in and out of the house all the time. It will be quite awhile before Aubrey will be in a big seat thankfully because we wouldn't be able to get past a rear facing car seat to get into the third row of the van. It is going to be quite the Olympic event trying to get back there to strap them in anyway.
We put them in our new quad stroller for the first time last weekend and they seemed to love it! I love the fact that I can take them out for a walk alone if Ben isn't home or he is busy. The babies are liking the warmer weather and taking walks, they look around at all the scenery and the fresh air seems to make them sleep a little better too.
It is so hard to believe that the babies will be 6 months next week. They are doing new things all the time. Blowing raspberries and chewing on their fingers are the games of the week so we almost always have bibs on them to keep them from drooling on their clothes. All of the boys are able to sit up by themselves for a few seconds at a time and Aubrey either doesn't have the desire to or can't yet. She thinks a Princess doesn't have to do anything she doesn't want to! They all have tickle spots and different toys that make them laugh. Coming soon...we will be feeding them cereal next week for the first time so I will be posting pictures of their first time eating cereal!


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Published: Wednesday 1st July 2015 by The News Editor
The United Nations says it is concerned about the state of the Great Barrier Reef and is urging greater efforts to conserve one of the most world's spectacular natural sites.
The decision, taken in Bonn, Germany, puts pressure on Australia to prevent further destruction of the 1,430-mile reef off the Queensland coast.
But Unesco's World Heritage Committee held off designating the site as endangered, pending further reviews.
Experts say the reef's unique ecosystem is threatened by climate change, waste water, fishing and coastal developments – including the use of nearby ports to export coal, which has led to dredging that kills valuable corals.
Australia's environment minister, Greg Hunt, told the meeting that his country had "clearly heard" the committee's concerns and would strive to implement a 35-year conservation plan.
Published: Wednesday 1st July 2015 by The News Editor