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Quinn Build Guide by Bunny Ripper
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Quinn Build
Starting Items
Damage core
Life steal choice
Defense choice
Example of final build
Ability Sequence
Not Updated For Current Season
The masteries shown here are not yet updated for the current season, the guide author needs to set up the new masteries. As such, they will be different than the masteries you see in-game.
Threats to Quinn with this build
|Ez. As long as you can position yourself against him you'll be fine. Try to E after he uses his Q to engage.
|Easy. He has no gap closers and the only thing he can do to you is knock you up. You can counter jungle him easily too.
|Easy for you. Your vault cancels his W and R if you do it correctly. Try to counter him early. He can't do anything
|Not really seen in the jungle anymore, but this is very easy for you. He's a very weak dog early game so counter jungling him will mess him up and make him useless early on. he has no gap closers and you can easily keep a distance. He has a slow and attack speed reduction but just vault away from him and wait for his W to go off.
|Not played too much in the jungle but she's easy. You can E away from her engage and the blind can mess her up.
Hello everyone. I am Pride, and this is going to be my super in-depth guide on the champion Quinn. In my opinion, Quinn is a very interesting champion with mobility, ganking, and burst potential that a lot of adcs don't have. Quinn is my favorite champion and I played tons of games with her and found many tricks on this champion.
I'm a person that lives in Boston, and my in-game name is Bunnie Boi NA server. My favorite champion is Quinn. So I'm making a guide about her right here. Although I'm currently Gold 3 I can still give you lots of information about this champion. I started in around the middle of season 4 and when I hit level 30, I immediately picked up Quinn, and fell in love with her. I played her top a million times until the rework, where I decided her best position should actually be mid.
In conclusion, I'm a Quinn main with lots of knowledge on her and I can give you good tips on this champion.
Now let's jump into this guide!
Pros / Cons
-Very Good mobility
-Strong dps and burst
-Very good ganker/counter ganker
-great duelist
-awesome split pusher
-Many different build choices
-Strong all phases of the game
-her W is very good for scouting for counterganks or countergank yourself
-Vulnerable to CC
-Ult doesn't deal too much damage
-Passive seems kind of random
-Not the best teamfighter
Summoner Spells
Almost all champions take flash. Quinn is no exception.
Since you're jungling you're gonna have to take smite. There's really no other summoners you can take except for exhaust which I personally don't like. Exhaust can help lock down champions you want to gank but imo it's really not useful
Standard adc runes. I take one attack speed quint for early clears, but more is not really necessary.
Start with a hunter's machete and three potions and a warding totem. After you clear, go for a skirmishers saber or the tracker's knife but personally I think the red smite is just too good to pass up. Finish it off as a warrior enchant as devourer got nerfed and then go for mobility boots. Quinn's ult goes really well with mobility boots but of vourse if you really want to, go for attack speed boots but I don't find them that useful. Then, choose an attack speed crit item of your choice but I like shiv and phantom dancer the most. Runnan's and Rapid fire cannon are also good options. I'd get an essence Reaver next because the cdr is very good on Quinn and I find it much better than infinity edge. Then choose a lifesteal item of your choice. I'd get bloodthirster 90 percent of the time because blade of the ruined king got really nerfed a lot. unless they have a bunch of tanks, I'd take bloodthirster. Finally get a defense item. Frozen mallet for kiting, GA if you die a lot, Banshees if they have lot of ap, and randuins if they have lots of AD, and you can get a qss if the enemy has lost of cc. You can also swap a lifesteal item for armor pen, but armor pen got nerfed and unless all the enemies get double armor items, i'm not going to get armor pen.
Skill Sequence
For the first clear get Q first since it blinds the monsters, deals aoe damage, and applies a passive mark. Next, I would get E, since it ups your clear speed with another passive mark and a little leap damage, and being an auto attack reset. But if there's an enemy that counter jungles with ease like a lee sin, I'd take W and use it when im midway thru my buff. And the passive helps clearing nice too. Then I'd max Q>E>W and get ult whenever you can
Cooldown:8 (goes down when you build crit)
Valor will mark an enemy as vulnerable and Quinn's next basic attack against the enemy will deal 15-100 based on level with an additional 116%-150% ad ratio based on level. Harrier can also be activated by Blinding assault and Vault.
TIP: This does lots of damage and is one of the main parts of your burst. Use this to your advantage! Also helps with early clears and remember, it gives vision!
Blinding Assault
Quinn throws valor in a line, stopping at the first enemy that gets hit, and deals physical damage in an aoe. Enemy champions hit by Blinding Assault have nearsight for 2 seconds and non champions are disarmed instead for 2 seconds. Physical damage: 20 / 45 / 70 / 95 / 120 (+ 80 / 90 / 100 / 110 / 120% AD) (0.5 AP)
TIP:This is your main poke spell. The blind also helps and when enemies get cloes to you, you can use it to save yourself. This is also your main aoe clear tool.
Heightened Senses
Range on active: 2100
Passive: Attacking harrier targets grants you attack speed and movement speed of 20/25/30/35/40% for 2 seconds.
Active: Valor reveals an area for 2 seconds.
TIP your main scouting tool, you can also proc harrier marks to again the extra attack speed to help push. This can also prevent enemies from counter jungling you too so make advantage of this ability.
Quinn dashes to an enemy, knocking them back a short distance and deals 40/70/100/130/160 (+20% bonus AD) physical damage. After the knockback, the target is slowed by 50%, decaying in 1.5 seconds, before leaping back to your maximum attack range.
TIP use this for repositioning, chasing, and fleeing from enemies. A lot of people aren't aware of the mini knockback and this ability can be used for Yasuo Last Breath and you can interrupt channels. This is why Quinn can be so good against champions like Katarina with channels. Useful tool for kiting jungle monsters too.
Behind Enemy Lines
After channeling for 2 seconds, Valor picks up Quinn, and they unite, gaining MASSIVE Total Movement speed 70/100/130%. Quinn then gets the ability to use skystrike. Taking damage from anything other than minions or using Blinding Assault, an auto attack, or vault will immediately activate skystrike.
TIP:This is what makes Quinn the roaming monster she is. Use this skill to gank lanes and to get back to the jungle after backs. You can get up to 700 movement speed at max rank with mobility boots and use this as an advantage.
Quinn lets go of valor, ending Behind Enemy Lines effect and you deal 100% of your total attack damage to all enemies around her.
TIP: I feel like skystrike isn't really that great. Used to, this was used as an execute ability but now, the only thing you can use it for is wave clear.
Playing Quinn in game
Early Game
Early game, you just want to do a standard clear. Start at your bot side since you get a better leash. On blue side go krugs>Red>Raptors>blue>gromp>Wolves. On red side start gromp>blue>wolves>raptors>red>krugs If you see any gank opportunities,gank. Quinn is very strong ganker pre six and post six so don't just farm all day. On these routes you'll be able to gank mid or top with ease. If the enemy is pushing, you can go in for free kills. When you go in try to vault in a position where you will land behind them. Combo your enemies by vaulting, auto attack, Q, auto attack. You can easily burn flashes and get kills with red.
mid game
Once Quinn hits six, time for ganking all over the place. Now you want to fully focus on ganking even more. Same combo but with your mobility you can get everywhere all over the place. After successful ganks, try getting objectives like towers or dragons/rift harold.
late game
Try to split push, as quinn excels in 1v1 duels and late game as long as you land your Q, you will obliberate every champion in a 1v1. Remember to proc your passive for extra pushing power. Sometime though, you have to teamfight. Try to play like any adc, just stay in the back, but instead of just attacking whoever you can, Quinn actually has a bit more burst and if you see a squishy walking around just burst them. Also before any teamfight, try to make advantage of Quinn's amazing pick potential and try to pick off an enemy
That was my Quinn guide! I hope you learned a lot from it!
I'll continue to update it and PLEASE comment. I would love some positive and negative feedback and it would help me a ton.
Enjoy playing Quinn! CAW CAW CAW
Contact Info
If you want to contact me, just email me here at email@example.com.
You can friend me on league too. Summoner name: Bunny Ripper. (sorry I'm only on NA)
Join my chatroom too if you want. Chatroom name: Bunny Ripper
Comment in the discussion Tab and I'll read every comment :D
Also a while ago I made a Quinn mid guide and here's the link to that! http://www.mobafire.com/league-of-legends/build/patch-5-24-caw-caw-the-ultimate-guide-to-qwuinn-all-your-ga-435255


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Ping to a Republican-American Editorial.
If you want on or off this ping list, let me know.
Ping to a Republican-American Editorial.
If you want on or off this ping list, let me know.
I was surprised Little Rock renamed its airport for BJ Clinton; I thought one comfort of losing the impeachment was that nobody would name anything after a president who settled a sexual harassment lawsuit for $890,000 after pulling his penis out on an unwitting woman (that wasn't one of his numerous girlfriends). The 5 year law license suspension should have made it official...
And Toe-sucker was right there alongside them.
One has to wonder...who could do that...who was more powerful than Bill and Hillary?
Someone apparently is.
I broke out my old video of the Clinton impeachment trial and enjoyed watching him squirm under the questions (he was under oath, but that did not slow down his lying).
But for a few pinko congressmen and our history could have had a very different outcome.
but it was nice to see that there were, and still are, a few folks that stand true to the Constitution and the moral law of our nation.
This guy and his wife slimed poor whites yet they'd still vote for him. Now the Little Rock airport is being named after them. I'm glad I stopped flying. I don't think I could stomach hearing the fight attendent say "Welcome to Bill and Hillary Clinton Airport".
America would be a better place if whoever was in the back seat that night with Clinton's mom and used a condom.
Clinton should stick to taking advantage of young female interns nearly 30 years younger than himself and masturbating in sinks. Nixon had the decency to resign in disgrace rather than get impeached. Not Clinton. He disgraced himself by vainly hanging onto power and actually getting impeached.
Witnesses bearing damaging testimony against the president were a favorite target of the Clinton IRS. Those singled out for audits include: Clinton paramours Gennifer Flowers and Liz Ward Gracen, sexual assault accusers Paula Jones and Juanita Broaddrick, and fired White House Travel Office Director Billy Dale.
Is Obama Using The IRS To Silence Opposition Voices?
Tea Party groups around the country alerting us to the oppressive demands being sent to them from the IRS.
The players are different but the game is the same.

subset: commoncrawl


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Haynes-Granville-Brown Session of Presentations by Recent Doctoral Recipients
What is the "NAM New PhD Session"?
The Haynes-Granville-Brown Session of Presentations by Recent Doctoral Recipients is a three-hour session held at the Joint Mathematics meetings to showcase the newest graduates in the mathematical sciences. This NAM session is named after the first three African-American women to earn doctorates in Mathematics:
- Martha Euphemia Lofton Haynes (September 11, 1890 -- July 25, 1980) earned her doctorate in 1943 from The Catholic University of America under the supervision of Aubrey Landrey with a dissertation entitled The Determination of Sets of Independent Conditions Characterizing Certain Special Cases of Symmetric Correspondences.
- Evelyn Boyd Granville (May 1, 1924 -- ) earned her doctorate in 1949 from Yale University under the supervision of Einar Hille with a dissertation entitled On Laguerre Series in the Complex Domain.
- Marjorie Lee Browne (September 9, 1914 -- October 19, 1979) earned her doctorate in 1950 from the University of Michigan under the supervision of George Yuri Rainich with a dissertation entitled Studies of One Parameter Subgroups of Certain Topological and Matrix Groups.
Each speaker in the Haynes-Granville-Brown Session is allotted 30 minutes, which consists of 25 minutes for the talk and 5 minutes for questions.
Speaking Awards
Over the years, the Mathematical Biosciences Institute (MBI) has been kind enough to offer speaking awards to the best presenter in the Haynes-Granville-Brown Session of Presentations by Recent Doctoral Recipients. Historically, the award provides (1) a certificate, and (2) reimbursement for the winner's expenses to attend one MBI scientific workshop during the 18 months following the New PhD Session.
At the 2015 JMM in San Antonio, we selected Pamela Harris (United States Military Academy) as the winner for her talk entitled On the Adjoint Representation of a Classical Lie Algebra and the Support of Kostant's Weight Multiplicity Formula. She attended the Dynamics in Networks with Special Properties workshop from January 25 -- 29, 2016. At the 2016 JMM in Seattle, we selected Nakeya Williams (United States Military Academy) as the winner for her talk entitled Modeling Head-up Tilt via an Optimal Control Approach and a Non-pulsatile Cardiovascular Model. Both of these award winners are currently listed on MBI's web site.
We will give out another Speaking Award in 2017. We will have a committee of three judges which will decide who should receive the award. No presenter is eligible for the award if the presenter is employed by or currently visiting the awarding Institute. The judges will use the following criteria:
- Visual Display (judged out of 10 points)
Were the slides easy to read? Was the text on the slides legible? Were there grammatical mistakes in the presentation? Were the graphics of good quality? Did the slides accurately display the main points? Were the visuals relevant to the exposition?
- Oral Presentation (judged out of 10 points)
Did the speaker make eye contact with the audience? Did the speaker enunciate? Did the speaker address the audience clearly? Did the speaker field questions from the audience adequately? Did the speaker appear to have a solid grasp of the material?
- Mathematical Content (judged out of 10 points)
Did the speaker discuss the relevance of the problem to the mathematical community? Did the speaker state the main results clearly and concisely? Did the speaker introduce directions for future research?
The winner will be announced during the NAM Banquet that evening.
Suggest Speakers for the Haynes-Granville-Brown Session
We welcome suggestions for future speakers in the Haynes-Granville-Brown Session of Presentations by Recent Doctoral Recipients. Please consider the following criteria:
- Speakers for the New PhD Session must be current members of NAM.
- We prefer speakers who have received their doctoral degrees in the mathematical sciences within the five years prior to the New PhD Session.
- We prefer speakers who have not given a talk in the Haynes-Granville-Brown Session of Presentations by Recent Doctoral Recipients in the past.
- We seek a diversity of speakers. Please remember that our intended audience will feature a diversity of race/ethnicity, gender, age, and subject matter.
E-mail your nominations here:
Past Haynes-Granville-Brown Sessions of Presentations by Recent Doctoral Recipients
2017 JMM: January 4-7 in Atlanta GA
Friday, January 6 from 1:00 PM -- 4:00 PM
A708, Atrium Level, Marriott Marquis Atlanta
Organized by Candice Price (University of San Diego) and Talitha Washington (Howard University), featuring talks by
- Deidra Andrea Coleman (Philander Smith College)
A Bayesian False Discovery Approach to Syndromic Surveillance
- Piper A. Harron (University of Hawaii at Manoa)
Equidistribution of Shapes of Number Fields of degree 3, 4, and 5
- Cris Negron (Massachusetts Institute of Technology)
- Valerie Nelson (Howard University/Department of Defense)
Existence Results for Some Higher-Order Abstract Differential Equations with Applications to PDEs
- Nourridine Siewe (NIMBioS)
- Ashley K. Wheeler (University of Arkansas)
Finiteness of Associated Primes of Local Cohomology Modules over Stanley-Reisner Rings
2016 JMM: January 6-9 in Seattle WA
Friday, January 8 from 1:00 PM -- 3:50 PM
Room 211, Washington State Convention Center
Organized by Candice Price (University of San Diego) and Talitha Washington (Howard University), featuring talks by
- Caleb James Ashley (University of Maryland at College Park)
- Syvillia Averett (Central State University)
- Reginald L. McGee (Mathematical Biosciences Institute)
Dynamic immunity: Mathematical models for B cell signaling pathways and chronic lymphocytic leukemia
- Kevin Muriithi Mugo (Purdue University)
- Nakeya D. Williams (United States Military Academy)
Modeling Head-up Tilt via an Optimal Control Approach and a Non-pulsatile Cardiovascular Model
2015 JMM: January 10-13 in Seattle WA
Monday, January 12 from 1:00 PM -- 1:55 PM
Room 215, Seattle Convention Center
Organized by Dawn Lott (Delaware State University), featuring talks by


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Costars by Claire Adams
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Bingo, Bridesmaids & Braces Trailer (1988)
05 October 1988 90 mins
The third in a series of films from acclaimed director Gillian Armstrong, Bingo, Bridesmaids and Braces captures with candid honesty, the lives of three working class women as they grow up from the "not so innocent" age of 14 to "it's happening too fast" at 18, through until "well, we survived" at the ripe old age of 26. We witness the women confronting the very real issues of teenage pregnancy, and love versus sex, marriage and career. Faced with the choice of swapping their earlier dreams for reality, they rapidly change their opinions on all that life has now delivered them.
Watch the official Bingo, Bridesmaids & Braces 1988 trailer in HD below or find more Bingo, Bridesmaids & Braces videos on Vidimovie.


text: Click the Link to watch: www.youtube.co.uk/KnobsCast The new series form the KnobsCast in this series we are playing a survival map called the Forgotten Land where we are trying to complete 6 dungeons and try not to die lets see how we get on. Let us know what you think any comments welcome. Please COMMENT, LIKE and SUBSCRIBE. www.youtube.co.uk/KnobsCast We are trying to boost are www.youtube.co,uk/KnobsCast channel why not subscribe to are channel and check out what we do and let us know what you would like to watch. Thanks for watching.


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Production of recombinant proteins for use as pharmaceuticals, so-called biopharmaceuticals, is a multi-billion dollar industry. Many different cell factories are used for the production of biopharmaceuticals, but the yeast Saccharomyces cerevisiae is an important cell factory as it is used for production of several large volume products. Insulin and insulin analogs are by far the dominating biopharmaceuticals produced by yeast, and this will increase as the global insulin market is expected to grow from USD12B in 2011 to more than USD32B by 2018. Other important biopharmaceuticals produced by yeast are human serum albumin, hepatitis vaccines and virus like particles used for vaccination against human papillomavirus. Here is given a brief overview of biopharmaceutical production by yeast and it is discussed how the secretory pathway can be engineered to ensure more efficient protein production. The involvement of directed metabolic engineering through the integration of tools from genetic engineering, systems biology and mathematical modeling, is also discussed.
The introduction of genetic engineering by Cohen and Boyer in 19731 laid the fundament for the current biotech industry, which is based on using microorganisms or cell cultures for production of proteins that can serve as pharmaceuticals, often referred to as biopharmaceuticals.2,3 A few years later, researchers at Genentech cloned the genes for human insulin and growth hormone, and expressed them in Escherichia coli,4 hereby demonstrating the utility and applicability of genetic engineering in creating genetically engineered bacteria that produce these two human proteins. In 1982 this led to marketing of the first biopharmaceutical, human insulin, by Eli Lilly, who licensed the technology from Genentech. In 1985 Genentech received FDA approval to market their own first product, Protropin®, the human growth hormone to be used for children with growth hormone deficiency. In 1987 this was followed by the tissue-plasminogen activator (t-PA, Activase®), another Genentech product, an enzyme that can resolve blood clots in patients with acute myocardial infarction. Also in 1987 Novo (now Novo Nordisk), a major insulin producing company, launched human insulin produced by the yeast Saccharomyces cerevisiae as a replacement for their human insulin enzymatically derived from porcine insulin. Shortly following these early developments many other products were launched and today there are more than 300 biopharmaceutical proteins and antibodies on the market with sales exceeding USD100B,5,6 with monoclonal antibodies representing the majority (> USD18B) followed by hormones (> USD11B) and growth factors (> USD10B).7 Furthermore, biopharmaceuticals have the fastest growth in the marked with an annual growth of about 19%,8 and there are currently more than 240 monoclonal antibodies and 120 recombinant proteins in clinical trials.9
About 40% of the biopharmaceuticals are currently being produced by mammalian cell cultures, mainly using Chinese Hamster Ovarian cell lines (CHO cells), as these allow for production of proteins with very similar glycosylation patterns as human proteins.10,11 E. coli is used as cell factory for production of another 30% of the biopharmaceuticals whereas 20% are being produced by S. cerevisiae10.11. The dominant biopharmaceuticals produced by S. cerevisiae are insulin (and insulin analogs), human serum albumin, hepatitis vaccines and virus like particles, e.g., for vaccination against human papillomavirus. The advantages of using yeast S. cerevisiae as a cell factory for the production of biopharmaceuticals are that this eukaryal model system enables production and proper folding of many human proteins. Furthermore, the proteins can be secreted to the extracellular medium and this facilitates subsequent purification. A further advantage is that in many cases yeast can perform proper post-translational modifications of the protein, including proteolytic processing of signal peptides, disulfide bond formation, subunit assembly, acylation and glycosylation.12 S. cerevisiae is also widely used as an eukaryal model organism13,14 and there is therefore much information available about this organism through high-throughput studies,15 databases, sequenced genomes and extensive toolbox for molecular modification, which provides an extensive knowledge base for further engineering of this organism. One of the limitations with the use of yeast is, however, that it performs high-mannose type N-glycosylation. This confers a short half-life of the modified protein in vivo, which then can have a reduced efficacy for therapeutic use.16 Much work has been performed on engineering yeasts, both S. cerevisiae and Pichia pastoris, so that they can carry out human-like N-glycosylation patterns that even includes terminal addition of sialic acid to the glycoprotein.16-18 This has opened for an even wider use of S. cerevisiae as a cell factory for production of biopharmaceuticals and there is therefore much interest in further engineering of yeast for ensuring efficient production of recombinant proteins.
More than 40 different recombinant proteins have been expressed, produced and secreted by S. cerevisiae.12 This includes several biopharmaceuticals and Table 1 provides an overview of some of these products, i.e., the protein name, their therapeutic application, leader sequence used and the titer reported in the publically available literature. As the table illustrates there are basically used three different types of leader sequences to ensure efficient secretion of the protein through the secretory pathway. S. cerevisiae only secretes few proteins to the extracellular medium, with the α-factor (a yeast hormone involved in mating) being the most studied and therefore most frequently used for efficient secretion of recombinant proteins. To further improve protein secretion in yeast Kjeldsen and coworkers at Novo Nordisk developed a synthetic leader that has been shown to be very efficient in protein secretion.12,19 As illustrated in Table 1, yeast can, however, also secrete human proteins that are expressed with their native leader sequences.
The secretory pathway in yeast is quite complex (see Fig. 1 for a schematic overview) as it involves more than 160 proteins that are responsible for different post-translational processes, e.g., folding and glycosylation. The secretory pathway handles more than 550 proteins that have a signal peptide in the yeast proteome, but only very few of these proteins are secreted to the extracellular matrix as all proteins targeted to the endoplasmic reticulum (ER), Golgi, vacuole and cytoplasmic membrane are also processed through the secretory pathway. Protein folding in the ER is of key importance for the secretory pathway as the accumulation of mis-folded proteins results in ER stress that is handled then by the unfolded protein response (UPR). Activation of the UPR results in transcriptional change of about 400 genes,20 many of which are under regulation of the Hac1p transcription factor.21 A result of this regulation is upregulation of chaperones and foldases as well as ER associated degradation (ERAD). Based on studies of the UPR many targets for improving protein secretion have been identified and implemented,12 and it is generally believed that any factor that reduces ER stress and its downstream damage caused by heterologous protein production, results in improved secretion of the produced protein.
Due to the complexity of the protein secretion pathway there has traditionally been focus on transcriptional regulation of protein production. A large number of different promoters have been evaluated for driving expression of the heterologous genes, and among the most widely used promoters are strong glycolytic promoters like pTDH3, pPGK1 or pTPI1, pADH1, or galactose induced promoters like pGAL1, pGAL7 or pGAL10.12 However, even though changing expression may improve production of one protein, it does not imply there would be improvement in production of another protein, as clearly demonstrated in our recent study on production of insulin precursor and α-amylase.22 In this study we used an expression system originally developed by Novo for their insulin production. This expression system involves deletion of the TPI1 gene in the chromosome and use of the corresponding gene, POT1, from Schizosaccharomyces pombe as a plasmid marker (see Fig. 2). This gives a very stable construct as all cells losing the plasmid will be deficient in triose-phosphate isomerase activity, a key glycolytic enzyme. A particular strength of this method is that it is stable also with use of complex media containing amino acids and nucleotides, which is not the case with standard yeast auxotrophy markers. Using this vector system we evaluated two different promoters (pTPI1 and pTEF1) as well as two different leader sequences (synthetic leader and the α-factor leader) for production of insulin precursor and α-amylase, and found that there was considerable differences in production of the two proteins with the different expression systems evaluated, i.e., with low gene expression α-amylase was produced at higher levels, whereas for high gene expression system insulin precursor was produced at higher levels.22 This pointed to very different protein processing in the ER, i.e., the larger and more complex α-amylase is more challenging for ER-processing when the flux is high. This hypothesis was confirmed by a measured increase in ER stress (by genome-wide transcription analysis) in the α-amylase producing strains. Based on this analysis it was hypothesized that engineering of the down-stream secretion pathway may be able to improve the secretion of amylase, and indeed overexpressing regulators of the so-called SNARE complex, Sec1p and Sly1p, resulted in improved protein secretion.23 Moreover, it was found that overexpression of SEC1, that is involved in regulating vesicle trafficking from Golgi to the cell membrane, resulted in improved production of both insulin precursor and α-amylase, whereas overexpression of SLY1, that is involved in regulating the vesicle fusion from ER to Golgi, increased only the α-amylase production.23 Through combined overexpression of the Sec1p and Sly1p the overall secretion of α-amylase could be improved by about 70%, whereas insulin precursor production was increased by about 30%, and the study therefore clearly demonstrates that engineering of the secretory pathway can result in significant improvement of recombinant protein production.
There are many other studies that clearly demonstrate that engineering of the secretory pathway can result in improved protein production,12 but a general finding is that most targets are quite specific, depending which particular protein is being overexpressed. Ideally, there would be one (or few) efficient yeast platform strain(s) that can serve as production host for a wide range of different biopharmaceuticals. There is therefore a need for a more rational approach to engineering yeast for improved protein secretion. In such an approach detailed models of the secretory pathway can be used for design, similarly as it has been done with engineering of metabolic pathways, where the use of genome-scale metabolic models has been shown to be of great importance.15,24,25 This approach is referred to as Metabolic Engineering, and the workflow, often called the Metabolic Engineering Cycle,26 is illustrated in Figure 3.
As illustrated, the workflow involves detailed modeling, often based on detailed analysis of the cellular metabolism and physiology using high-throughput experimental techniques developed in the field of genomics, and the concept of quantitatively describing cellular processes with mathematical models is at the core of systems biology.27 The metabolic engineering cycle is therefore very similar to the workflow of many systems biology studies where perturbation of the cellular system is performed using genetic engineering, e.g., by overexpression or deletion of specific genes, followed by detailed analyses that can be used to define a mathematical model of the biological system. However, there is a major difference in the sense that systems biology is a fundamental science where the primary objective is to gain novel insights, whereas metabolic engineering is an applied science with the primary objective to obtain an improved cell factory. Clearly the process of metabolic engineering also results in improved insight of the cellular metabolism and physiology, but generally to a less extend than in a systems biology study due to the differences in study design resulting from the different objectives. A major limitation in using the rational Metabolic Engineering approach, with model based design, for improving protein secretion, is the lack of detailed models of the protein secretion pathway. Even though there have been described several kinetic models,28,29 these describe only part of the pathway. There is therefore an obvious need for a genome-scale model for protein secretion, in analogy to what has been done for metabolism.30 Such detailed models will not only enable rational design of improved protein secretion routes they will also enable improved integrative analysis of this complex pathway using previously developed tools (for studying metabolism31) and hereby lead to more insights into how the secretory pathway operates as a system.
I would like to thank my students and post docs working in the field of protein production by yeast for fruitful discussions and collaborations: Dr. Jin Hou, Dr. Keith Tyo, Dr. Jose Martinez, Dr. Mingtao Huang, Zihe Liu, Lifang Liu, Tobias Österlund and Amir Feizi. I also would like to thank Prof. Dina Petranovic for excellent collaboration and constructive comments on this paper. Research in the area of yeast protein production in my group is funded by European Research Council (grant no. 247013) and the Novo Nordisk Foundation.
Previously published online: www.landesbioscience.com/journals/bioe/article/22856
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text:
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text:
Proverbs 13New International Version - UK (NIVUK)
13 A wise son heeds his father's instruction,
2 From the fruit of their lips people enjoy good things,
3 Those who guard their lips preserve their lives,
4 A sluggard's appetite is never filled,
5 The righteous hate what is false,
6 Righteousness guards the person of integrity,
7 One person pretends to be rich, yet has nothing;
8 A person's riches may ransom their life,
9 The light of the righteous shines brightly,
10 Where there is strife, there is pride,
11 Dishonest money dwindles away,
12 Hope deferred makes the heart sick,
13 Whoever scorns instruction will pay for it,
14 The teaching of the wise is a fountain of life,
15 Good judgment wins favour,
16 All who are prudent act with[b] knowledge,
17 A wicked messenger falls into trouble,
18 Whoever disregards discipline comes to poverty and shame,
19 A longing fulfilled is sweet to the soul,
20 Walk with the wise and become wise,
21 Trouble pursues the sinner,
22 A good person leaves an inheritance for their children's children,
23 An unploughed field produces food for the poor,
24 Whoever spares the rod hates their children,
25 The righteous eat to their hearts' content,
New International Version - UK (NIVUK)
Holy Bible, New International Version® Anglicized, NIV® Copyright © 1979, 1984, 2011 by Biblica, Inc.® Used by permission. All rights reserved worldwide.
You'll get this book and many others when you join Bible Gateway Plus. Learn more


text:
With Respect to Sex is an intimate ethnography that offers a provocative account of sexual and social difference in India. The subjects of this study are hijras or the "third sex" of India, individuals who occupy a unique, liminal space between male and female, sacred and profane. Hijras are men who sacrifice their genitalia to a goddess in return for the power to confer fertility on newlyweds and newborn children, a ritual role they are respected for, at the same time as they are stigmatized for their ambiguous sexuality. By focusing on the hijra community, Reddy sheds new light on Indian society and the intricate negotiations of identity across various domains of everyday life. Further, by reframing hijra identity through the local economy of respect, this ethnography highlights the complex relationships between local and global, sexual and moral, economies.
This book will be regarded as the definitive work on hijras, one that will be of enormous interest to anthropologists, students of South Asian culture, and specialists in gender, queer, and sexuality studies.
"This book is an important and significant one. Not only does it add to the field of academic accounts of the hijira and anthropology, but it contributes to gender and sexuality studies."
Shane Gannon


text:
|09 May 2014
Installing windows 7 home premium on blank HD, missing drivers.
Hey guys, my hardrive burnt up so I went to a computer repair shop to see if He could check it out, when we were 100% it was done for he had a blank laptop hard drive he could sell me with a burnt windows 7 disk or charge 75$ extra to install it for me. I of course took the blank HD with the installation disk and went home and started it, but when I started the installation I got to the part where it requires drivers but it didn't find any on the disk.
My question is, since I'm going to have to get drivers, can I use a flashdrive to install the drivers? And if so, do I have to format the flashdrive or anything so it will read the drivers?
|My System Specs
|09 May 2014
The laptop itself is a...
Hp Pavilion g7 Notebook
Product number A7A44UA#ABA
I downloaded the graphics and audio drivers, along with the wifi one, but are there any that it absolutely has to have in order to work? I've read elsewhere about a chipset driver, but I cannot find it on the HP website.
Once I get it started i'll be using public wifi to get the rest of the drivers as well via auto update im assuming, or I can just download the rest as i see fit.
|My System Specs
|09 May 2014
Just got back home and popped in the flash drive with the drivers I downloaded from hp-Hewlett, and the laptop cant find them, it shows all the folders from my other things but not them, anyone know as to why?
|My System Specs
|09 May 2014
As long as you have your wifi driver, you can get the rest later if needed.
Regarding the installer asking for drivers, I would start over with,
Clean Reinstall - Factory OEM Windows 7
Using a fresh download of the install media in step one.
|My System Specs
|09 May 2014
As long as you have your wifi driver, you can get the rest later if needed.
Regarding the installer asking for drivers, I would start over with,
Clean Reinstall - Factory OEM Windows 7
Using a fresh download of the install media in step one.
After downloading them, I didn't click on them, do I have to install them or something first onto the flash drive in order for the computer to find and read them? Because there's a option to "hide drivers that are not compatible with hardware on this computer" and even when I click to see all drivers, even non compatible ones, nothing shows up.
|My System Specs
|09 May 2014
Since it doesn't tell you what drivers it wants, it's hard to advise further, to be honest you shouldn't need to.
The reason I suggested a fresh download was to be sure you had an up to date installer, free of possible corruption.
Try working through these steps,
Windows 7 Installation Failure - Overcome
|My System Specs
|09 May 2014
Wel I tested the hardrive in the BIOS and got Quick (303) 1 which when I googled it, is apparently saying the hardrive is no good, I put the CD into the desktop on my other 2 hardrives that need a OS as well and get the same "No device drivers were found. make sure that the installation media contains the correct drivers, and then click OK." Message, not sure what to do with that link you sent me with just the installation disk and flash drive though. Probably just going to try and find a desktop on craigslist or something for its Hardrive with a OS already on it since I cant get my desktop or laptop to read the drivers on the last 2 flashdrives I've used.
|My System Specs
|09 May 2014
I would not give up on that PC. If the HD is connected correctly and is not being detected in BIOS setup or by a good Win7 installer, then take it back and have the store replace their bad merchandise. They can't rip you off like that and get away with it as it's theft.
The error message you're getting means the installation media is also bad, so the store needs to either make good their bad merchandise and installation media or refund your money.
If you have a flash stick you can download the latest official installer for your licensed version by clicking here Win7 Home Premium x64 SP1 English Official ISO download to write it to a 4gb flash stick using the tool provided in Windows 7 USB-DVD Download Tool . Format the stick first by plugging it in to rightclick on it at Start>Computer to Format. Once it's written, you can again download your wireless driver to it from the HP Support Downloads webpage for your model PC. If there is more than one Wireless LAN driver download them all to the stick.
Now boot the stick using the HP F9 key, follow the illustrated steps in Clean Install Windows 7.
Once its installed if it's not ready to connect to network, install the wireless LAN driver to connect, enable Automatically deliver drivers via Windows Update (Step 3) then Check for Updates, install all Important and Optional Windows Updates, with reboots, until there are no more offered when you go back to Check for Updates.
Any drivers then still missing in Device Manager can be found on the Support Downloads webpage for your model. We can help you find any that are elusive.
If you stick with the tips, tools and methods in Clean Reinstall - Factory OEM Windows 7 you'll get and keep a perfect install.
|My System Specs
|10 May 2014
Alrighty, I found a "HP pavilion all in one" computer for 100$ on craigslist, thing runs great so hopefully tomorrow I'll be able to format my flashdrive now since I couldn't at the library, thanks for all the help guys!
|My System Specs
|10 May 2014
So what are you doing with the laptop?
|My System Specs
|Thread Tools
|Similar help and support threads
Reinstalled windows 7 home premium 64 bit missing drivers.
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Windows 7 Home Premium (64-bit) need help with drivers.
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Fresh boot of Windows 7 Home Premium, drivers not installing correctly
As of yesterday, my HP Envy 6z-1000 laptop acquired a hive error. Unable to fix it, I simply installed a clean boot of Windows 7. In doing so, the "Recovery Partition" that comes factory installed on the computer was formatted and I no longer have access to the Recovery options provided by HP. I...
Blank UI (tabs, windows...) on Windows 7 Home Premium
Well, a friend of mine gave me his laptop to fix with a strange error I've never seen or heard of. The UI of Windows simply doesn't have any letters, and every window you open is blank (this is best appreciated on the attached images). The only windows you can see letters on, are the Command...
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Drivers for windows 7 home premium
HEY.I was wondering if it is possible to update my drivers free,and if it is possible then where and how kind regards asser:confused:
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text:
Models For Upper Canopy 430ba
Upper Canopy 430ba by Indesit
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Email me when back in stock - we'll try to re-stock quickly!
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text:
The following factors could affect Italian markets on Tuesday.
Reuters has not verified the newspaper reports, and cannot vouch for their accuracy. New items are marked with (*).
For a complete list of diary events in Italy please click on .
ISTAT releases April producer prices data (0800 GMT).
Italy sells 6.0-7.5 billion euros over three bonds at auction, including 5- and 10-year nominal bonds.
Corriere della Sera said indications coming from the EU Competition watchdog highlighted "several difficulties" in reaching a positive outcome over the rescue of the two Veneto banks.
Italy's fourth biggest bank Monte dei Paschi di Siena said it was in exclusive negotiations with a domestic fund and a group of investors over the sale of its bad loan portfolio, which it needs to offload to be allowed to receive state aid.
The bank, which at the end of last year had 26 billion euros in gross defaulting debts, has set a June 28 deadline for the talks with Quaestio, the fund which manages banking industry rescue fund Atlante and which will also conduct negotiations on behalf of other investors.
(*) The bank has managed to halve the request of 10,000 job cuts put forward by the EU Commission but for CEO Marco Morelli it would be necessary to spare another 1,500 units, Il Messaggero reported. The paper said Monte dei Paschi is also seeking to reduce the number of branches to be shut down to 550-600, from a downward revised request of 800 at present.
(*) The size of a planned 450 million euro share issue could rise to 600 million euros, Il Messaggero reported. The paper said Carige's board was expected to approve at a meeting on Tuesday the sale of 950 million euros in bad loans using the GACS state guarantee at a price equivalent to 31 percent of their nominal value. Bankers from Credit Suisse, Deutsche Bank and Goldman Sachs are expected to attend Tuesday's meeting.
Both Il Messaggero and Il Sole 24 Ore reported the bank's top investor Vittorio Malacalza, backed by co-shareholders Volpe and Spinelli, is at odds with CEO Guido Bastianini over issues including the spin off of a further 2.4 billion euros in bad debts. A due diligence conducted by Prelios on the portfolio to be spun off has highlighted the need for further writedowns, Il Messaggero said. Il Sole said the board was also divided over the possibility of converting an 80 million euro subordinated bond held by insurer Generali into equity.
A deadline for EU Competition authorities to rule over whether French media group Vivendi has de facto control over Telecom Italia expires on Tuesday.
Vivendi could consider replacing TI's Chairman Giuseppe Recchi with its CEO Arnaud de Puyfontaine if the European Commission ruled in its favour, la Stampa reported.
The group's Aeroporti di Roma unit started on Monday a European roadshow for the launch of a 10-year bond for up to 500 million euros that will run until Thursday, Il Sole 24 Ore reported.
The shipping group and the oil and gas group have signed a memorandum of understanding to cooperate over energy systems and in particular the transport chain of natural gas.
Board meeting to approve 2017-2023 strategic plan (press release and presentation on May 31).
Annual general meeting (0600 GMT).
Annual general meeting (1200 GMT).
For Italian market data and news, click on codes in brackets:
20 biggest gainers (in percentage)............
20 biggest losers (in percentage).............
FTSE IT allshare index
FTSE Mib index........
FTSE Allstars index...
FTSE Mid Cap index....
Block trades..........
Stories on Italy...... IT-LEN
For pan-European market data and news, click on codes in brackets: European Equities speed guide................... FTSEurofirst 300 index.............................. DJ STOXX index...................................... Top 10 STOXX sectors........................... Top 10 EUROSTOXX sectors...................... Top 10 Eurofirst 300 sectors................... Top 25 European pct gainers....................... Top 25 European pct losers........................ Main stock markets: Dow Jones............... Wall Street report ..... Nikkei 225............. Tokyo report............ FTSE 100............... London report........... Xetra DAX............. Frankfurt market stories CAC-40................. Paris market stories... World Indices..................................... Reuters survey of world bourse outlook......... Western European IPO diary.......................... European Asset Allocation........................ Reuters News at a Glance: Equities............... Main currency report:...............................


text:
Public sector jobs on line despite €531m in savings
THE Government yesterday refused to rule out further pay cuts for public servants in the forthcoming Budget -- despite workers hitting the savings targets in the Croke Park deal.
A new report published yesterday said that €298m had been cut from the public sector pay bill, and another €308m clawed back in reforms, but further savings were needed to help meet the terms of the deal.
And the first progress report also shows that the public sector pension bill has increased by €385m over the past two years -- an increase of 14.3pc.
Public Expenditure and Reform Minister Brendan Howlin last night said the Government would set new targets for more savings on top of the €531m delivered by the Croke Park agreement over the 12 months up to last March.
This will include up to 25,000 further job cuts over the next four years, the sharing of administrative staff and the scrapping of more quangos.
But he warned that unless the savings needed under the IMF-EU bailout were achieved, more pay cuts could be on the cards.
"If we don't get the savings voluntarily, then we have to resort again to pay cuts. That issue is the bottom of the Government's agenda, it's a place we don't want to go to. But we have to have the savings," he said.
The Croke Park pay and reform deal was thrashed out in March of last year after weeks of intense negotiations.
It promised not to hit state employees with any more pay cuts until 2014 if extensive reforms in work practices were implemented, which would lead to savings of €1.2bn.
The first report on its implementation said it was on track, but pointed to a €385m rise in the public sector pension bill over the past two years.
Most of the savings were generated by the drop in the number of staff employed in the public sector, as well as reduction in overtime.
Among the areas where more needs to be done are:
Working Hours
There is still no standardised working week across the public service, and extra working hours in universities have not been delivered. Garda rosters need to be reformed, and there is a need to cut allowances to members of the Defence Forces.
Major changes to rosters, staffing levels and work practices are needed, particularly in the health sector, where introduction of extended working hours including a seven-day working week and 8am-to- 8pm working day must be implemented.
Better Public Services
More services, such as applying for third-level grants, should be available online.
Some functions, such as pay and pensions and human resources, will have to be merged among departments.
There is a need for more civilians to be employed in the Garda Siochana, Prison Service and Defence Forces, along with a "radical restructuring" of local government.
Most of the staff redeployed under the Croke Park Agreement have gone from the health service to the Department of Social Protection.
Some 1,000 community welfare service staff have moved from the HSE to the department, with another 507 coming from other government departments. There will be a need for "significant redeployment" across all departments.
Staff Reductions
Some 5,349 people have left the public service, the bulk of which came from the health sector (4,180). This led to a cut in €289.2m from the payroll bill, but the State still spent €15.712bn paying 303,457 public servants last year.
Management numbers have also fallen, down 7.4pc, but there has been an increase in front-line health staff.
Another €85.7m of expenditure was avoided because of more efficient practices.
Between 22,000 and 25,000 more jobs will go by 2015.
Other savings
A total of €308m was saved last year by cutting travel expenses, selling assets and closing public offices.
Better prices for supplies such as stationary and office supplies saved €39m, while local authorities cut €81m from their day-to-day spending.
The HSE saved a total of €126m, including €18.5m on medical and surgical supplies and €7.5m on catering. Overtime payments are down by 5.2pc overall -- saving €6.3m -- while sick leave has fallen by 13pc in the Department of Education.
Irish Independent


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02-06-04, 20:33 #1Registered User
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- Feb 2004
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- Philadelphia
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Unanswered: Unable to browse cube in Analysis server
Hi All
I ve installed SQL ANalysis server and was able to connect properly. I have installed the latest SP3 for both the SQL Server 2000 and Analysis Server and went through the entire process of creating the cubes as specified in the Analysis Server tutorial. I can view the cube but cannot browse it, the error returned is Unable to browse the cube <cube name> :unspecified error" . Anyone faced this problem and know whats wrong ,pleasee let me know!! greatly appreciate it ..thanks alot..


text:
1920s KNICKERBOCKER FELT KRAZY KAT DOLL WITH WIRE SPRING WHISKERS
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- Sold Date:
- Source: eBay
I believe this to be a Knickerbocker Cloth/Felt Krazy Kat doll circa 1920's/1930's, but I am not certain. Very old and very good quality cloth construction. Wire spring whiskers and black bead eyeballs. Arms have bendable wire in them. Seams splitting in some areas (left shoulder and rear, where tail is missing) that shows what appears to be human hair stuffing. I had a very early Donald Duck with the long beak that I sold on eBay over 15 years ago that looked very similar in style and construction to this. Anyone who may have any information about the manufacturer or the cartoon character this depicts please contact me so I can update/correct the description. Bidding starts at $49 with NO RESERVE. High bidder to pay $12 for pro packing, shipping and insurance.


text: NRL position on Dally M awards Thu 28 Aug, 2014, 12:20pmNRL Tweet Email Print Twelve past and current Cronulla Sharks players have been suspended for breaches of the game's anti-doping rules. Credit: Grant Trouville. Copyright: NRL Photos. The NRL have outlined the restrictions which will apply to players who have been suspended for breaches of the game's anti-doping rules. Chief Operating Officer Jim Doyle said the players will be ineligible to receive a Dally M Award or the Ken Stephen Medal in line with the rules for those awards. It will be a matter for other leagues and clubs to determine whether the suspended players are eligible for awards, such as the Brad Fittler Medal, which come under their jurisdiction. Players will continue to be recognised for career achievements in the usual way. Tweet Email Print Send to a friend: Your Name: Your Email: Friend's Name: Friend's Email: Close Submit loading image needs to go here


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Sunny Leone's Anti-smoking Ad is something you must watch, this will make you kick cigarette right now
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Sunny Leone, Deepak Dobriyal and Alok Nath team up for the best ever anti-smoking short film and it delivers a clever message against smoking. Smoking hot Sunny Leone looks cute with Deepak Dobriyal and Babuji Alok Nath is adding more glitters to the video. If this doesn't make you quit smoking, nothing will. Watch the video right now...
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Top 10 Treks for Solo Travellers
There's no need to be nervous about daunting travel destinations anymore.
Just because your friends can not join you it does not mean you have to miss out on a fantastic adventure holiday. Group travel is safe and more popular than ever. Our friends at KE Adventure Travel tell us nearly 56% of their travellers are independent, with no extra supplement for booking solo. Take a look at their top 10 adventure trekking holidays perfect for those wanting to travel alone and share the experience with like-minded walkers.
Imagine relaxing in the lodge in Namche with your new found friends chatting about the first ever view you had of Everest. This trekking holiday is not only one for the bucket list, but it's a fantastic holiday to meet other trekkers every night in your mountain lodge accommodation. With mind blowing views every step of the way this KE Bestseller will ensure you have amazing memories to take home and share.
KE's classic Moroccan trek is the perfect holiday to meet fellow travellers whilst staying in locations popular with other international trekkers. The excellent local guides are renowned for making sure everyone is looked after from start to finish and this adds to the all-round social experience. Everything is taken care off right down to enjoying the dramatic scenery and summit of Mount Toubkal! With all meals and airport transfers included, plus plenty of 5* reviews, One Week Toubkal is the perfect choice!
Another KE Bestseller, this holiday always has good group sizes. It's popular with single travellers and although you may be really sociable, you may also want your own space. So, there is a single supplement option to have your own room and tent throughout the whole of this holiday. Summit Ras Dashen with likeminded travellers, enjoy superb trekking and UNESCO site visits all in the safety of local English speaking guides.
You'll stay in mixed accommodation on this holiday so you'll definitely have a sociable time during this challenging trek which walks the complete route from north to south. Exchange email addresses while enjoying the views from the granite peaks over the dramatic coastline, as you trek across the spine of this beautiful island.
Wild Albania is a remote, beautiful, intriguing and mind-blowing destination; you will never receive a warmer welcome than from the local people here. You can opt for a single room in the hotels, but most of the accommodation throughout the trek is shared as you stay in traditional mountain homestays and village accommodation where you'll enjoy real local hospitality. No need to worry about exchange rates after Brexit, this holiday is all inclusive* from the minute you land.
9 days, departures Jun - Sep. From £595 Land Only
*excludes miscellaneous expenses – drinks souvenirs etc
With #Bigwarmhug being the latest message from Tenerife there is no need to feel alone on this 'all year round' sunshine island. Can't get your friends to join you for a weekend away? Then why not head off for your own for a short break. "What did you do at the weekend?" "Oh I watched the sunrise from the highest peak in Spain and you?" Jealous - they will be!
Another iconic trek for the bucket list – but without the crowds on the main Inca Trail! While relaxing in the mess tent, you'll find yourself chatting over the highlights of your day with your new found friends. Was it the dramatic views down the Apurimac canyon, the incredible sunset on the Llama terraces or the anticipation of Machu Picchu that is the topic of conversation?
Bhutan is a Shangri La, but with strict rules for visas and travel it is not the easiest of countries to visit. However, KE's classic holiday which treks across three high passes and of course visits the famous 'Tiger's Nest' Monastery, takes care of all of that for you. You can just relax and enjoy this spectacular circuit trek in the comfort and safety of your fully guided group.
This winter adventure is great fun for independent travellers. It is only 3 hours from the UK, fully guided and has single room options throughout. Go on the hunt for the Northern Lights, hike over glaciers, watch geysers explode and visit glittering Ice caves; This holiday is jam packed with experiences to share with your fellow travellers and also includes the impressive Gullfoss waterfalls and a few nights in Reykjavik!
If you are worried about your first solo adventure, then the Singalila Ridge is perfect for a first time trekker in the Himalaya. It is easy walking following good trails and the altitudes are not extreme. There is lots of social time in the mess tent when you're camping and some great guided sightseeing when in Darjeeling. Everything is taken care of on this holiday, from the airport transfers and internal flights to all the accommodation, meals and sightseeing tours.
A little preparation can help you feel more secure about travelling alone. KE has also put together answers to some of the most commonly asked questions, to help you feel more secure about travelling as a solo traveller.


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[dropcap]A[/dropcap] quick look at the future of regenerative medicine.
Ultimaker is a Dutch company producing affordable, open source 3D printers. They have close ties to local hackerspaces and eagerly promote collaboration and innovative, open uses of their product. Recently, they shared a story of a Dutch medical researcher, Ernst Jan Bos, who is using the Ultimaker 3D printer to create "scaffolding" upon which new human body parts may one day be grown. Using adult stem cells taken from a patients, new body parts can be grown and then grafted onto the patient.
Ernst Jan Bos (in Dutch) is experimenting with ears, with burn patients in particular in mind. In the video below, he can be seen explaining the process (in Dutch), which includes taking a 3D scan of an existing body part, transferring it to the Ultimaker for printing, then using it as the basis for creating molds for growing the ear.
Video: Dr. Anthony Atala of the Wake Forest Institute for Regenerative Medicine explains the groundbreaking work he has been involved with over the years. The promise of regenerative medicine will have profound implications, transforming beyond recognition our modern perception of healthcare. A longer talk:
The limitations to the paceofprogress placed upon Wake Forest are many fold.


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This is truly living life on the edge. Forget those regular retirement plans, veteran Bryson William Verdun Hayes, is setting the bar higher than ever. Literally. Known as Verdun, the great-grandfather broke another record just the year before when he skydived for the first time last year at 100 years old, which broke the British record for oldest skydiver. But that that record wasn't enough for Verdun who, took the world record from former holder Armand Gendreau at 101-years-old and three days by skydiving at 101-years-old and 38 days.
Hayes said he's wanted to skydive since he was 90 but was talked out of it by his late wife. You could say he's just catching for lost time now.


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2012 Performances
Sat May 12
The Meatmen
The Pyramid Scheme
Sat Jul 28
The Meatmen
Cobra Lounge
Sat Aug 18
The Meatmen with Negative Approach
Magic Stick
A The Meatmen setlist is a compilation of all the songs The Meatmen has played live in concert. Many The Meatmen concerts have the same setlist, and sometimes The Meatmen setlists will vary from night to night. We've gathered together 2017 The Meatmen tour setlists as well as past The Meatmen setlists to give you a better idea of what you can expect at your local The Meatmen show. If you have a The Meatmen song list you would like to upload, please check back as that functionality will be added soon, as well as setlists for The Meatmen.


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Chekhov, The Apocalypse And Minimalism: Haiku Reviews (Huffington Post)
HAIKU REVIEW: Roberta Price, whether she meant to or not, compiled one of the great photographic documentations of the American hippie lifestyle in the late 1960s and '70s. Resident of and frequent visitor to the communes that sprouted amongst the edenic (if hardly forgiving) mountains and valleys of Colorado and New Mexico, Price regarded her fellow ruralists as friends and family and co-conspirators, but shot them also with the analytical eye of an anthropologist. In other words, these are no mere snapshots of a remarkable era, they are detailed records of a movement and even a method. As such, "Across the Great Divide," Price's photo-journey through the Rocky Mountain high, provides resonant backdrop to the efforts at communal structure, post-capitalist economy, and do-it-yourself ethics - all formulated in the guise or name of art - assembled downstairs as "Worlds Outside This One." If Price's show looks at time and place, "Worlds," bringing together quasi-practical stabs at idealism from around the globe, looks at timelessness and placelessness - the world as commune.
- Peter Frank


text: I Am Seeking a Slim Loving Partner Me Too A nice broad minded fit nudist would be ideal 2 People Report Group Sort popular recent I like smaller women. I am a big muscular man and I like the feeling of power I have with a tiny little woman. StrongProud 66-70, M 2 Jan 15, 2014 More Stories


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Mamma Mia! | London West End
Novello Theatre - Current Show: Mamma Mia!
5 Aldwych, London, WC2B 4LD - Novello Theatre Tickets: 0207 759 9611
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Mamma Mia!, London West End
George and Ira Gershwin's hit musical comedy is packed full of classic songs including "I Got Rhythm", "Someone To Watch Over Me", "Embraceable You" and "Nice Work If You Can Get It". The original 1992 production won both the Tony and Olivier Award for Best New Musical.
The creative team from the multi-award winning production of Hello, Dolly! reunite as Timothy Sheader directs, with choreography by Stephen Mear and designs by Peter McKintosh.
Gareth Valentine returns as the musical director after joining the Open Air Theatre last season for Sondheim's Into the Woods.


text: The history and legacy of One Bad Pig has already been established. They have been the very best at what they do with their dynamic and explosive performances, their on-purpose, profound and in-your-face music, lyrics and stage antics that have entertained and influenced people of all faiths and walks of life around the world. This band is still honored and revered by fans, other musicians, producers, label executives, journalists and critics throughout the music world. After all these years they still have people everywhere that want to emulate them. People still love 'One Bad Pig'. They have earned their place in music history by just being themselves and doing what they do as well as anybody. Here are some examples of some of their on stage antics, like shooting silly string, skateboarding on-stage, smashing guitars, stage-diving into a kiddie pool of what looked like ice cream and whipped cream, popping fireworks onstage, inviting members of the audience to join them on-stage to sing or dance and screaming out mini-sermons on stage. The wild antics that this band performed will live in the hearts and minds of people everywhere forever. Respectfully, Carl Phelan.


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Alder Safety offers a wide range of training and consultancy services from Basic first aid, Fork Lift Truck, IOSH course, risk assessment and many other industrial training courses. All of these services are delivered by seasoned professionals.
We provide a bespoke service to your business whether you are new and in start-up, need to make changes to your current set up, or perhaps keep up to date with the raft of new employment legislation.
Courses can be delivered in-house to ensure they are tailored to suit the needs of your business
These courses can be delivered at weekends or nightshift at no EXTRA cost
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September 18, 2008 at 5:22 pm #86251
@geoffcouch wrote:
Gordon Brown inherited a strong financial position. He then sold gold reserves after telling everybody he was going to do so. .Naturally the price of gold fell heavily. He then changed the tax situation on pensions, precipitating the demise of final salary pensions.(This was against expert advice)
You forgot to mention the £20,000,000,000 (yes billion) he also received from selling the 3G mobile phone licences


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Riyadh in no position to speak on behalf of women at UN: Analyst
Members of the European Parliament have slammed as repugnant and unacceptable the April election of Saudi Arabia to serve on the UN women's right commission. The UN vote, which paved the way for Riyadh, viewed as the world's leading oppressor of women, to sit on the council prompted outrage from human rights groups worldwide. Press TV has asked Catherine Shakdam, director of the Shafaqna Institute for Middle Eastern Studies from London, and Jihad Mouracadeh, lecturer and political analyst from Beirut, to express their views on the issue.
Shakdam said that the Saudi kingdom has no right to represent women in the international community, because the regime does not even recognize the basic rights of its own female citizens.
"Saudi Arabia does not recognize women as human being," the expert said on Wednesday night.
The regime in Riyadh "is in no position to be speaking on behalf of woman," because women in the kingdom are "less than nothing," and are treated as "commodities," she noted.
In recent years, Saudi Arabia has come under intense pressure by rights groups for mistreating women. In 2016, the kingdom was ranked 141 out of 144 on the Global Gender Gap Index.
Shakdam went on to say, "Saudi Arabia right now is lobbying enough Western capitals, Washington and London of course, to try to push their agenda and to try to reach the highest office in the international community."
"The United Nations has no clue whatsoever" and "the only thing that the UN is responding to right now is money and how much political attraction one can have," the analyst complained.
The international community "is desperately trying to present and portray Saudi Arabia as a legitimate power," but the reality is that the state is a reactionary power "living and breathing on sectarianism" and denying the rights of religious minorities, ethnic groups and women, she argued.
"When you have a country such as this to be sitting anywhere in the international community, is an insult to the rest of the world," she added.
Meanwhile, Jihad Mouracadeh said, "Within the rules of the United Nations, women's rights are very different from what they are in Saudi Arabia."
He said there are different rules and regulations when it comes to treating women throughout the world and in various countries.
"You cannot impose on Saudi Arabia the rules that are existing in England or in France," Mouracadeh explained.
Therefore, he urged, "One must respect the way people deal with women as long as women's rights are not subordinated."


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General Details
Bachelor in Creative Industries, Major in Music
Learn to play guitar or bass in whatever style you like. Practice your favourite songs or learn to write your own rock, punk, metal or fusion songs, with all the hints and techniques along the way!
These details were last updated on May 1, 2013


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.- A committee of Christian parents submitted a petition to the Quebec government, with more than 56,000 signatures, in favor of keeping Catholic and Protestant religious instruction in the province's public schools.
The petition was presented in the Quebec legislature on behalf of the committee March 24 by Mario Dumont, the leader of Action démocratique du Québec.
Quebec's public schools have been fully secular since the passage of Bill-118 in 2000. However, the government invoked the Charter's notwithstanding clause and granted parents the legal right to choose between Catholic and Protestant religious instruction and moral instruction for their children.
The clause expires June 30 and parents want the government to renew it for another five years.
However, the government-appointed Religious Affairs Committee has recommended that the government not renew the clause.
Rather, it has recommended that the Ministry of Education replace the current three-option system with a class for all students that would teach about world religions and encourage students to discern which religious tradition they prefer.
This course is clearly being imposed against the will of the parents, said committee president Jocelyne St-Cyr at a press conference March 23.
She pointed to statistics from Quebec's Ministry of Education that show that 80 percent of parents choose Catholic and Protestant religious instruction for their children in elementary school and 60 percent choose it at the high school level.
The committee had a 75-minute meeting March 11 with Education Minister Jean-Marc Fournier to present a brief and discuss their position. The minister told the parents that the government has not yet made a decision on whether or not to renew the clause.
Quebec's Liberal government is expected to enter debate on the notwithstanding clause in May.
Petitions are still circulating. The committee pledged that it would forward these petitions to the National Assembly.


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Oakland, Berkeley, And East Bay News, Events, Restaurants, Music, & Arts
Page 2 of 4
Might Chris Thompson's feelings about Bishop Cordileone be unduly harsh? The article read like a call for the villagers to grab their pitchforks and torches and storm the cathedral at Lake Merritt. It's fair enough for Thompson or the Express to have such views, but it's worth considering that the pressing issue for many Prop. 8 supporters is not so much same-sex marriage but, rather, a change in the nature of marriage itself. If California were considering three-way marriages, or marriages to beasts or to objects, then we'd surely see a Prop. 9 or Prop. 10. In other words, the issue isn't "gay" marriage but "changed" marriage.
I think the only way to escape the endless bickering is to separate marriage from the legal system. I believe Michael Kinsley was the first to propose this, in his 2003 Slate article deceptively titled "Abolish Marriage." If marriage no longer carried legal "rights," then it would become completely cultural. Each religious or cultural group could decide for itself which type of marriage(s) are encouraged. One church might marry same-sex couples, another might not. Even if the marriages had no legal significance, they'd still have cultural significance — much like a Bar or Bat Mitzvah, or a Confirmation.
Are there any organizations in the East Bay working for a separation such as this? If so, I'd be interested in reading about them in the Express.
David Goldweber, Oakland
Shame on You, Pro-Degenerate Media
It's a pathetic display to see how the pro-degenerates media tries and distorts the meaning of the victory in the ballots of Proposition 8 by assigning credit for it to a single Catholic bishop. Where was Salvatore Cordileone when we, the voters, passed Proposition 22? The only reason for Proposition 8 not winning by a larger margin was because Jerry Brown, intentionally, changed the wording forcing us to vote "Yes" instead of "No to gay marriages" as it was originally intended, thus confusing a lot of people. Twisted logic and labels of "bigots, right wingers, religious nuts, etc." are not going to prevent us, the workers, from defeating them again, whether in 2010 or 2012 for the degenerations of nature are always going to be rejected by the species. It's an instinct for self preservation.
Leo T. West, San Leandro
"Parking Rebellion Stirs Up Oakland," News, 8/12
Time for a Lawsuit
My dispute with the City of Oakland over a parking ticket was described as follows: "The citation ... said that she had been parked on Waverly for more than two hours, but Seritis claimed that the parking log's initial time was incorrect."
This is in error. I had, for the second time in the past year, received a ticket after properly moving my vehicle from 23rd to Waverly within the two-hour limit posted on these streets. When I requested the activity sheet for this ticket, it showed that my vehicle was originally spotted on "23rd / Waverly / 27th / Harrison." In other words, if I had parked on ANY of these streets, and then moved anywhere within this four-block zone within the two-hour limit, I could have received a ticket for a two-hour zone violation. This is in direct violation of the city's own Oakland Municipal Code 10.28.190, which restricts the two-hour limit to a SINGLE street. I appealed this ticket twice and was turned down both times, in spite of the fact that the sheet clearly shows that my initial location was not identified as any one street, but could have been 23rd, 24th, 26th, 27th, Waverly, or Harrison. This has also happened to several of my co-workers, some of whom were mentioned in the same article.
I think it is significant to note this to your readership because it shows that the City of Oakland is illegally setting people up for tickets they do not deserve, and then making it nearly impossible to protest these tickets and have unfair citations overturned. Where does the city define its two-hour zones? Is it five blocks in some areas? Is it ten? Is it an entire neighborhood? And since this violates their own city law, why are our first appeals rejected automatically, without anyone even looking at or acknowledging the evidence? My first rejection stated that I should submit evidence to back my claim, which I already had but which was ignored. Even my second appeal was rejected, despite the fact that their backup for my initial location was woefully inadequate.
What the city is doing is clearly abusive, and should be stopped by the citizenry. Perhaps a class-action lawsuit? I would be a willing participant.
Karin Seritis, Alameda
Do You Ever Feel Like You've Been Had?
Of all the problems that Oakland has, now we have a parking crisis? What does one say? This parking crisis is one of our own making. It is hard to stay respectful and polite when our members of the city council do something so short-sighted. It is not just the 50 cents an hour; it is not just the extra hours until 8 p.m.; it is not just the higher ticket prices. It is the feeling that "gotcha" and anything-goes-for-a-buck is the way they feel. That is what gets under the skin and make us feel like a crime victim when we get a parking ticket.
They sometimes call us shoppers, sometimes call us residents, sometimes call us taxpayers, but they sure do not treat us like citizens. They treat us like someone to milk because they get away with it.
Most of our council did not run in a contested race to get their job. All but one of the council members represents a district that has nothing to do with where the people of Oakland live, except to make sure that some of those who live in the hills live in each district. But how do they get so out of touch with what it is like to live and work here?
A friend was telling me yesterday that a customer who got a ticket while in her cafe came back into the cafe and told her that she will never come back again. The cafe owner is in no way responsible for Oakland parking policies, but the anger and frustration of the client was very understandable. We who run businesses in Oakland get to hear how angry the citizens who get caught in the trap feel.
Parking tickets were never a very friendly way to raise funds. The way Oakland does it is something of a trap. It is set up to make it hard on the driver and increase the opportunities to ticket rather than collect parking fees.
Contact the author of this piece, send a letter to the editor, like us on Facebook, or follow us on Twitter.
News - November 22, 2:18 PM
News - November 22, 10:25 AM
News - November 17, 10:50 AM


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Welcome to GM Authentic Autographs!Information About this Item: Autograph By: Bella Thorne Item: 8x10 Shake it Up photo in blue ink Location of Signing: while out in NYC to promote Shake it Up (specific details will be given to the buyer) Shipping: $6 (US Priority Mail) International Shipping via USPS Global Priority Mail varies by country. Includes a signed Certificate of Authenticity from GM Authentic Autographs
Who We Are…Together Greg and Matt have over 15 years of experience dealing and selling authentic autographs. They obtain each individual autograph in person and provide a detailed description of where the item was signed on a GMAA Certificate of Authenticity. Unlike most of the items that tend to hit eBay today, we make sure that each and every item that we offer was obtained in person, by us. While most of the autographs in our inventory are obtained in New York City, GMAA also travels to select film festivals and conventions from time to time. Why Our Product? While only some of our items come with "proof" pictures (that is, a photo of the person signing the item), we offer a money back guarantee that EVERY one of our items is 100% Authentic. There are many fake items on eBay, and few sellers who are as passionate about obtaining their autographs in person as we are. ... read more


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Matchday 19 in Serie A only saw 18 goals over the 9 matches with five of those coming in Verona as Atalanta thrashed Chievo 4-1. We take a look at who is leading the top scorers in the league.
Gonzalo Higuain scored twice as Juventus beat Bologna 3-0 at home. The Argentine had opened the scoring after just seven minutes, Massimiliano Allegri remain four points clear of Roma with a game in hand.
Higuain now has 12 goals in the league this season, with the striker netting five in his last three matches.
He has the second-best minutes-per-goal ratio in the league, scoring every 103 minutes; only Dries Mertens can better that.
New year, same @G_Higuain! It only took six minutes for the @juventusfcen man to open his account for 2017. #SerieA https://t.co/b3jgpNX6GA— BT Sport Football (@btsportfootball) January 8, 2017
Higuain was the only player in the top five to score this week, but in sixth is Ciro Immobile.
The Lazio striker scored a 90th minute goal as his side beat Crotone 1-0, they sit fourth in the table only one point behind Napoli.
Immobile has 10 goals now in Serie A, this was the first time he's scored for seven matches. His last goal came against Sassuolo back in October.
Ciro Immobile breaks a 7-game drought with late winner for Lazio - you can forgive him for getting booked for the celebration. He's back!— Alasdair Mackenzie (@aksmackenzie) January 8, 2017
Top Five Leading Scorers in Serie A
1. Mauro Icardi (INT) – 14
2. Andrea Belotti (TOR) - 13
3. Edin Dzeko (ROM) - 13
4. Gonzalo Higuain (JUV) – 12
5. Dries Mertens (NAP) - 11


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- Hawk HPS Front Pads for 05-09 Scion tC
HPS - High Performance Street disc brake pads are world renowned for increasing stopping power on your street legal vehicle. Increasing the performance of your vehicles braking system is easy when you choose Hawk Performance's HPS braking compound. This unique Ferro-Carbon formula was developed for street performance using the safety and quality of Aerospace and Motorsports severe-duty friction technology.
The High Performance Street compound offers a higher coefficient of friction over stock brake pads and can provide you 20-40% more stopping power and higher resistance to brake fade than most standard replacement pads. Less fade means you will have a highly durable brake pad with less brake dust.
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The drawing always comes first.
The thing is, I don't need you, Richie. I just need your legs.
I've never been to Oklahoma
but I live with its past. It shares our bed.
3 heart shaped daffodil bulbs
having sprouted all on their own
left nested in the dark
forgotten in the stillness
of an otherwise empty clay pot.


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Plus Size Embellished Full Finger Ring
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A ring and midi ring are connected with an embellished leaf design on top for a stunning full finger ring!
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Charlotte Russe Plus Size Embellished Full Finger Ring A ring and midi ring are connected with an embellished leaf design on top for a stunning full finger ring! 302240562 6.0 6.0


text: I'm extremely turned on by anal, however my girlfriend is quite a bit less experienced than I, even so she's a great lover, listens and attempts to accommodate even though sometimes a bit shy. I've been kind of wanting to propose the idea of anal sex or see what she thinks about it, but have been in no rush, didn't want to pull out all the cards right away.... She ended up asking what I thought about it/if I had tried it, this weekend while camping... I was quite surprised, she told me she had tried it once loooong ago (negative experience), however would be willing to try it with me. BROWNY POINTS!