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Cambria hotel & suites Rockville
Select your stay dates to check availability
Cambria hotel & suites Rockville
About the hotel
Property Location
With a stay at Cambria hotel & suites Rockville, you'll be centrally located in Rockville, steps from Peerless Rockville Historic Preservation and minutes from Beall-Dawson Historic House Museum. This hotel is within close proximity of Stone Street Medical Museum and F. Scott and Zelda Fitzgerald Gravesite.
Make yourself at home in one of the 140 guestrooms featuring refrigerators and flat-screen televisions. Complimentary wired and wireless Internet access keeps you connected, and cable programming provides entertainment. Private bathrooms with bathtubs or showers feature complimentary toiletries and hair dryers. Conveniences include desks and coffee/tea makers, as well as phones with free local calls.
Rec, Spa, Premium Amenities
Don't miss out on recreational opportunities including an indoor pool and a 24-hour fitness center. Additional features include complimentary wireless Internet access, wedding services, and a television in the lobby.
Enjoy a satisfying meal at a restaurant serving guests of Cambria hotel & suites Rockville. Quench your thirst with your favorite drink at a bar/lounge. Breakfast is available daily for a fee.
Business, Other Amenities
Featured amenities include a 24-hour business center, a computer station, and express check-out. Planning an event in Rockville? This hotel has facilities measuring 2136 square feet (198 square meters), including meeting rooms. Self parking (subject to charges) is available onsite.
Hotel Facilities
- Indoor pool
- 24-hour fitness facilities
- Computer station
- Number of buildings/towers - 1
- Conference space size (meters) - 198
- Luggage storage
- Wedding services
- Free WiFi
- 24-hour business center
- 24-hour front desk
- Television in lobby
- Restaurant
- Conference space size (feet) - 2136
- Smoke-free property
- Coffee/tea in lobby
- Self parking (surcharge)
- Multilingual staff
- Year Built 2015
- Elevator/lift
- Total number of rooms - 140
- Free newspapers in lobby
- Number of meeting rooms - 3
- Bar/lounge
- Safe-deposit box at front desk
- Breakfast available (surcharge)
- Number of floors - 8
- Express check-out
- Meeting rooms
Room Facilities
- Bathtub or shower
- Free wired high-speed Internet
- Climate control
- Daily housekeeping
- Hair dryer
- Connecting/adjoining rooms available
- Flat-panel TV
- Refrigerator
- Premium TV channels
- Private bathroom
- Free cribs/infant beds
- Coffee/tea maker
- Free WiFi
- Free long-distance calls
- Free toiletries
- Desk
- Iron/ironing board
- Cable TV service
- Free local calls
- Blackout drapes/curtains
- Phone
Hotel Policy
Know Before You Go
- One child 16 years old or younger stays free when occupying the parent or guardian's room, using existing bedding.
- The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.
The following fees and deposits are charged by the property at time of service, check-in, or check-out.
- Breakfast fee: between USD 5 and USD 20 per person (approximately)
- Self parking fee: USD 15.00 per night
- Pet fee: USD 15 per pet, per night
The above list may not be comprehensive. Fees and deposits may not include tax and are subject to change.Show all hotel policy Show less hotel policy


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Medical Definition of BMJ
BMJ: The British Medical Journal. The BMJ states that it "aims to help doctors everywhere practice better medicine and to influence the debate on health." To achieve these aims (the BMJ) publishes original scientific studies, review and educational articles, and papers commenting on the clinical, scientific, social, political, and economic factors affecting health.
"The BMJ is published weekly and has a circulation of about 115,000, of which 20,000 copies are distributed outside Britain. In addition, local editions reach another 185 000 readers." The BMJ is one of the major general medical journals in the world.Source: MedTerms™ Medical Dictionary
Last Editorial Review: 7/1/2016
Medical Dictionary Definitions A - Z
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eMedicineHealth Top News

subset: commoncrawl


text:
This was actually one of my first (and only ) decent short eared owl in flight shots....
Just a little help.....This week I received some wonderful news:
Out of thousands of beautiful photographs My fox in the snow photo was selected to be one of the finalist in the Dutch National Geographic Competition 2012...!:excited::boogie:
Which means that I'm very close to winning a new DSLR....!
For all people who happen to like my fox photos, or any person that just grants me a new camera....
All I need now is as many votes as I can get and maybe you guys will help me to win this so badly needed new cam...
My old one is about to retire and without a cam, I just wouldn't know what to do...
So I hope you'll forgive me for this 'begging' and don't mind to
It's amazing how large its wings are in comparison to the rest of its body.
two questions:
1. what camera/ lense did you use?
2. what did you do to this picture in editing?
1. what camera/ lense did you use?
2. what did you do to this picture in editing?
FABULOUS picture!
Reminds me of a barn owl
really kewl!!!
looks very nice!!!
looks very nice!!!
Splendidly beautiful!
Amazing capture!
Beautiful shot!
I love it's wings!
Great shot! :]
Great shot!
I don't know how where you live but there sure are some awesome animals nearby....nor do I know HOW you manage to take awesome shots like this but keep it up!
Good work!!!
Wow! Great! Oo
Gorgeous! I adore how you've captured this. It's so beautiful and amazing <3
Awww. I love short eared owls. Great pic.
How do you get that deep depth of field??
wow, incredible shot.
This is gorgeous! Those eyes are amazing.
ABSOLUTELY GOREGOUS!!! you captured all the beauty and gracefullness of owls in this one shot LOVE IT <3
beautiful! <3
perfect shot
amazing and beautiful, great shot
A beautiful and imposing sight!
Beautiful animal and what a great shot!
You bring the magnificence of this preditor into focus. WOW! Really, JUST WOW!
wow love it!
Great light and composition
Beautiful, amazing and stunning.
this is amazing!
I like how you see the movement in the wings
This is incredible! Which lens did you use?
Great photo !
What a wingspan on that bird! Very nice shot too.
Love the shot. It's really nice.
Very beautiful! I love owls too!
Such a beatutiful picture!
Nice shot! ^^
OMG!! I love owls!!!! THIS IS AMAZING
Amazing owl photo!!
Such an intense, clear shot. Exquisite!


text: Stolen Car (Serge Pigeon) ft Telefan on Guitar by telefan published on 2012-11-19T22:54:43Z Serge created this amazing track. I feel so fortunate to sit in on it. Thanks Serge! Check out Serge's other tracks: http://soundcloud.com/serge-pigeon Genre 70'S Police movie flick Comment by telefan @simone_nurra: :)))Thank you kindly from Serge & Jim! :)))) 2014/08/14 21:20:39 +0000 Comment by simone_nurra amazing groove... and this guitar has got a beautiful sound ever :)) 2014/08/14 19:02:19 +0000 Comment by telefan @roger-b-train: Roger :-))) Thank you my friend...Serge is the best. This was fun! 2013/12/05 00:02:45 +0000 Comment by Roger/B-Train Sweeet collab!!! lil Santana feel goin on!!! Great Job Serge and Jim!! :))) 2013/12/04 23:30:01 +0000 Comment by telefan @fb6960: Thank you! :-D 2013/02/26 18:37:56 +0000 Comment by Cozy Suginami great guitar play ! 2013/02/26 17:54:28 +0000 Comment by telefan @puppywolf: thank you ! :-D 2013/01/05 23:00:37 +0000 Comment by Puppywolf Studios @telefan: Great little groove there Jim.. nice work lads 2013/01/05 21:51:09 +0000 Comment by telefan @jaydee25: Thank you! Definitely check out Serge's page too! 2013/01/05 13:36:36 +0000 Comment by Jaydee25 Nice guitar ... sounds WOW! 2013/01/05 05:06:51 +0000 Comment by telefan @user884420902: Serge is an amazing musician! Definitely check out his page! Thank you from us both! 2013/01/01 17:24:35 +0000 Comment by Kees Winsemius That's some nice bass & guitar playing! 2013/01/01 17:21:24 +0000 Comment by telefan @scottbooth: thank you! Serge composed this and I sat in. Definitely check out Serge's page... A very talented man! 2012/12/04 00:50:52 +0000 Comment by Scott Booth Awesome tune!!! 2012/12/04 00:49:09 +0000 Comment by telefan @kellyanne-1: Thank you! It's been amazing for me...I have tremendous respect for Serge! 2012/11/27 02:28:04 +0000 Comment by KellyAnne I have to say, Serge is one of my favorite recent discoveries on SC. He is awesome. But don't sell your contribution short here—you are another fav discovery and this collab is *magic*!!! :) 2012/11/27 02:05:19 +0000 Comment by telefan @sierrajessicajohnson: Serge is really good! :-D 2012/11/26 20:01:58 +0000 Comment by telefan @sierrajessicajohnson: Sierra <3 thank you...that means a lot to me! 2012/11/26 20:00:56 +0000 Comment by Always~Falling~on~My~Face Great job jim :) 2012/11/26 19:21:58 +0000 Comment by Always~Falling~on~My~Face Dat phat bass :D <3 2012/11/26 19:21:15 +0000 Comment by telefan @rothwhyler: Thank you so much! Serge came up with the idea...he is an amazing musician! 2012/11/24 18:45:20 +0000 Comment by telefan @stephenthesaint: Serge came up with this...he really kills it on the bass! Thank you from us both! 2012/11/23 16:23:56 +0000 Comment by st. stephen man, is this something or what? love it from the get go! 2012/11/23 16:08:59 +0000 Comment by telefan @lee-lucas: thank you from us both Lee! It means a lot to me...much respect! 2012/11/23 04:17:03 +0000 Comment by telefan @lee-lucas: Thank you Lee! Serge brings the funk! He is just amazingly talented! 2012/11/23 04:15:38 +0000 Comment by Lee >>> Lucas Cracking little ditty and a top job by your both here JIm mate. Very well liked this well funked up little ditty, and hats off to you both of coarse on this fine work and track. 2012/11/23 00:21:21 +0000 Comment by Lee >>> Lucas Very tasty and even the Fuzz have got funk here in this weeks episode, and that;s a fair cop and well tasty stuff going down here guys... 2012/11/23 00:19:26 +0000 Comment by telefan @randy-jackson-1: Serge is an amazing musician...this was a joy for me! Thank you Randy! 2012/11/22 13:48:50 +0000 Comment by telefan @sas1976: Serge came up with idea...he is an amazing bass player! Thank you from us both! 2012/11/21 14:03:39 +0000 Comment by sas1976 GReaaaat!!! would fit in a movie like "shaft"!!!! :) 2012/11/21 13:32:58 +0000


text: Join team Highway Shoguns and secure a ranked spot before multiplayer is here! Requirements: Car must be 1000hp or more Must run 9.000 or less on the 1/4 !!No vehicle restrictions!! Send me a message or reply to this if interested. Include screenshots of garage with up rating or a summary of a race to show time.


text: Hi guys, I'm looking to grow my commercial mowing and plowing business and have been considering working with one of the national property managers. Can anyone recommend a good contact or company - any advice or feedback is appreciated. Feel free to email me at my private address if you prefer to keep your comments offline, Seth no email addresses please Charles


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Mass Effect 2
Glowing eyes and you!
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- Boards
- Mass Effect 2
- Glowing eyes and you!
Technically they're not "glowing", more like reflecting light:
when you're in a dark place there's no glowing:
While it may not be perfect, some scenes look just awesome:
note: the color can be any possible one from the spectrum, yellow/golden eyes make Shep look like a space vampire.
If anyone's interested in the exact values needed for this trick, ask away and I'll make a small guide for it.
Brainhunter 7 years ago#2
Is this mod scaled with your Paragon bar?
It would be awesome to see Shepard's eyes tingle with a faint blue light when your character is slightly Paragon, and vivid like Renegade Shepard's eyes when he's become fully aligned towards one attribute.
PSN ID - NemesisHunter (Mic User)
Very interested. Please post the specifics to achieving this effect.
GT: lJustAlexl
(those are lowercase Ls)
CheeseItBaggins 7 years ago#4
Regardless of whether or not I'll use it, this is really awesome, and should've been in the game from the start.
First get Gibbed's Save Editor rev25 fom http://mod.gib.me/masseffect2/saveedit_rev25.zip and extract it to a folder.
Open the program and then open a save file
The saves are located in your documents "Bioware/Mass Effect 2/Save/<character>/", I used the quicksave. Remember to do a backup first.
Go to the "Raw" tab, then 2. Squad > Player > Appearance > Morph Head > Vector Parameters and click the "..." button
A window will appear with a few parameters for your head, skin tone, eye white color (which can be modified for solid-looking eyes with this same method), hair color, etc.
You're interested in "EYE_Iris_Colour_Vector", click on it then click on the + on the right side
Here you have the values for the eye color in simple RGBA
A is Alpha, leave it alone; Red Green and Blue are what you want to modify
- - - - -
Use this website to get the proper value for the exact colors you want:
On the drop-down list next to "Select your data color space..." select "RGB 0-1"
The 3 fields below it represent Red Green and Blue, maximum for each field is 1.0 minimum is 0.0.
The blue that I used in my first examples would be [0.1] [0.4] [1], gold would be [1] [0.8] [0.3], then multiplied by 100 for the save editor.
- - - - -
Modify the values to your heart's content then click on OK and overwrite the old one (you made a backup hopefully).
I hope Imageshack keeps these up long enough ¬_¬
I was just playing with this myself.
Hit the Raw tab and scroll down to Squad -> Player -> Appearance -> Morph Head -> Vector Parameters. Click the [...] button and another window will open.
On the left side of the second window, find the EYE_Iris_Colour_Vector entry. (It's number five on the list, at least for me.) Then, on the right side, hit the plus sign next to Value. The menu will expand. The R, G and B entries are for red, green and blue, and presumably go from 0 to 255 (but I haven't tested going higher) and higher numbers mean more glow. The character in the pic above has B 228, G 6 and R1.
Right below that one, you'll find HED_Hair_Colour_Vector. As you may guess, it controls hair colour (the character in my pic has a 1 in every field for white hair).
I believe the default values are all below 1 (typically something like 0.431212), so anything from 1 and above may have a tendency to look glowy under certain conditions. But then again, that may be exactly what you want.
"Starting a war is not a rational thing to do. Cool off in the dungeon for a while!"
Heh, and someone else goes and posts instructions while I'm fiddling about, and does it better too. Figures.
"Starting a war is not a rational thing to do. Cool off in the dungeon for a while!"
Heh, I'm an image guy, easier to understand.
Anyway, playing with the skintone and eyewhite colors nets some interesting effects.
Fancy playing as Dr. Manhattan? (cba to search for a bald head mesh or to start a new char just to demonstrate this)
Oh and you can go much farther than 255, although the effects don't seem much too different. The maximum number for standard colors is 1, minimum 0.
Still, if it's just the eyes you want to modify you don't need a lot to look like Muad'dib.
Also note, the same thing works for the armor colors in the Coalesced.ini. Any number past 2 for the colors will make the armor/armor decorations glow in the light.
In this image I made a base absolute black color (R=0,G=0,B=0,A=1.000000) and a glowing blue decoration color (R=0.40,G=2.85,B=9,A=1.000000)
I want an edit button dammit >.<
i would've have liked paragon eyes
but nope, being good is too boring
"First you take a drink, then the drink takes a drink, then the drink takes you." - F. Scott Fitzgerald
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- Glowing eyes and you!
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text:
Gustafson Brothers Service Center
ReScore Reviews™ (2)
99% Would Recommend
Latest Review 2 days ago
Review for Gustafson Brothers Service Center
Alice E. on 09/03/2013
Alice said she really loves how the business handles and takes care of her and her husband. They make it a very easy check in and check out. She dropped her vehicle off the night before and specified a time she needed it by. They were wonderful and had the vehicle ready before the time she needed. She and her husband just bought a vehicle over the weekend and was offered a maintenance warranty. They declined because they would rather take their vehicle to this business. They have weekend hours and they are so accommodating. Julie is the best and has great customer service.
Category: Service
Review Created: 09/03/2013
This review was collected via phone interview by Customer Research Inc.


text: Hi Guys, Anyone out there have some spraying/fall fertilizing to do? Check this out: http://www.z-spray.com/max.html A little late in the year for half of you, I know, but this is a "rush" job, so I sure would like to find someone immediately who can put this machine through it's paces. Drop me an email with your name, address and phone and tell me what you have lined up that could use this gear. email@example.com Thanks! David C Exec Editor


text:
A Man Named Pearl Trailer (2006)
Man Named Pearl tells the inspiring story of self-taught topiary artist Pearl Fryar. It offers a message that speaks to respect for both self and others, and shows what one person can achieve when he allows himself to share the full expression of his humanity.
Watch the official A Man Named Pearl 2006 trailer in HD below or find more A Man Named Pearl videos on Vidimovie.


text:
Phone number: (03) 5991....
Email: Click to view
URL: http://www.freewayford.com.au
Business Info
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Some tips when posting a review: When writing a review, see if you can be as balanced as possible. What did you like, what didn't you like? Here are some things to think about - How was the customer service? How was the product or service quality? How was the price/value? Please also check and adhere to the editorial guidelines when posting a review.
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text:
Download EBOOK Immunology: An Illustrated Outline PDF for free
Category: Science & Geography
The author of the book: David Male
Format files: PDF, EPUB, TXT, DOCX
The size of the: 642 KB
Language: English
Edition: Garland Publishing Inc
Date of issue: 30 July 2013
Description of the book "Immunology: An Illustrated Outline":Immunology: An Illustrated Outline is both a review guide to the essential principles of immunology and a concise dictionary of immunological terms. It can be used as a review tool in preparation for course exams and medical licensing exams, or as a refresher when this content is encountered in related life science areas, such as microbiology and virology. The book also enables readers to look up specific terms in the index and locate the definitions, making it a powerful reference for interns, residents, and physicians in clinical practice.
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text: On Wed, Jul 23, 2008 at 11:51:40AM -0400, Cole Robinson wrote: > I've been working on a virtinst API to build and install xml > for libvirt storage objects. The current version is attached: > so far only nfs, filesystem and dir pools are implemented, as > well as their associated file volumes, but the remainder will > be mostly a cut and paste job. I have some UI wizards for > building these in virt-manager mostly complete, so this has > been tested to be pretty solid, though there is still some clean > up that needs doing. I think this is generally sound - the proof of any such API is how well it suits its users, so if it works well for virt-manager and virt-install CLI then go for it. > The general workflow is as follows: > > ========================================= > import virtinst.Storage.StoragePool as sp > > # This gives the appropriate class for the specified pool type > pool_class = sp.get_pool_class(sp.TYPE_FOO) > > # Only required params are a conn/uri and name. Default formats > # and target paths have default values, but source paths/ > # devices and hostnames obviously have no sensible default, but > # they still aren't required for object instantiation > pool = pool_class(name="foo", uri="xen:///") Should proably allow a existing connection to be passed in, otherwise this class has to deal with authentication issues too. Daniel -- |: Red Hat, Engineering, London -o- http://people.redhat.com/berrange/ :| |: http://libvirt.org -o- http://virt-manager.org -o- http://ovirt.org :| |: http://autobuild.org -o- http://search.cpan.org/~danberr/ :| |: GnuPG: 7D3B9505 -o- F3C9 553F A1DA 4AC2 5648 23C1 B3DF F742 7D3B 9505 :|


text:
What's it about: Trapped in a citadel swarming with Daleks, the Time Lord rescue force must find a way to overcome insurmountable odds. With the Daleks apparently planning to rule the Null Zone, perhaps their thirst for universal conquest and victory has been quenched… The War Doctor doesn't believe so — but how can he prove it without destroying any chance of peace? As the countdown to the destruction of Keska proceeds, a deadly choice must be made... A choice that will define this Doctor, and perhaps forever cast him in the role of 'monster'.
Damaged: Given Hurt's involvement and the fact that the War Doctor is supposedly the living embodiment of what the Doctor is not about I would have thought this set would have taken more time to explore his character. It pays lip service to his 'monstrous' characteristics (if you can really call pushing a button for the greater good a monstrous act...I guess you can in historical context) in a scene at the climax where somebody has to commit the act of devastation bit beyond that there was little to distinguish him from the others. Perhaps that is still to come. If it were any other race in the universe I am sure the Doctor would be an advocate for peace but he knows that you cannot make a deal with the Daleks. His incredibly sarcastic delivery of the shock news that the Daleks want to win the Time War after all is delightful, probably the most Doctorish thing he has said in the entire set. Doing what is necessary to save lives does not make you a monster. At least that is what the Doctor wants to tell himself. Ollistra taking the Doctor to Keska in the future is a kind of reward for his efforts throughout this set. He might not have been able to save everybody but thanks to his efforts the planet keeps turning.
Standout Performance: Celia Imrie delivers a very powerful speech about the horror of the Daleks. Oddly me it reminded me of the moment when her character went off on a mad rant in Dinnerladies and was similarly impressive and unexpected.
Sparkling Dialogue: 'The Daleks are not your glorious Overlords! They are simply your executioners!'
'Where can I find you Doctor. When I need you again?' 'At the heart of the battle where the blood of the innocents flows and only the monstrous survive.'
Great Ideas: It has a great cover and a great synopsis, after two less than motivated adventures there as every chance this could be the highlight of the set. A very important question is posed early in this story; would you sacrifice an entire race to destroy your enemy? The Time War can never be won and so Ollistra has put plans in place to negotiate peace with the Daleks. My jaw might have hit the ground when I heard that. Not because it was an unexpected development but because I had no clue how such a advance could be implemented. I mean, we're talking about the Daleks. It would have to be extreme circumstances to convince the Daleks to stop killing, peace could only be brooked at the threat of total annihilation. An inner sanctum of Time Lords who have come to the much more tasteful conclusion that peace has to be made with the Daleks. The Time Lords gave the Daleks the Null Zone weapon, a piece of hardware of their design from the Omega arsenal. It was supposed to obviate the loss of their main space time fleet and give them a chance of a bolt hole, a contained area of a thousand worlds where they hope the Daleks will be content. It's a bold idea, if a little deluded. If you think you are the ultimate example of racial purity in the universe you are not going to be happy with one small corner of the universe. You are going to want to spread and conquer. And what about the people on those thousand worlds? Sacrificed to the Daleks to slaughter them as they please. Why are the Daleks drilling into the cores of all these planets? Moveable bases from which they can administrate their new Null Zone Empire. A section of space with moving worlds like spaceships? That's pretty awe-inspiring. I've never heard anything quite like that before.
Isn't it Odd: Now I can see the comparisons with Dalek Empire that people have been making. Instead of the Dalek Empire just call it the Null Zone Empire. There is a terribly confused action sequence where the Doctor and his friends attempt to take on a Dalek that is firing with gay abandon. Nick Briggs has directed enough Big Finish action commendably for this to be a little bit embarrassing. It was all the voices that were thrown into the fracas, it's all a bit of a kafuffle.
Standout Scene: The Daleks want to fire a thousand planets at fifty times the speed of light at Gallifrey. That's a hell of a punch in the face. Talk about being hoisted by your own petard, Time Lords! I tell you what, Nick Briggs knows how to think BIG. This is a terrifying, Star Wars scale notion or the sort of thing that Douglas Adams might think up if he was having a particularly sadistic day.
Result: The box set could have been re-branded The Story of Keska rather than Only the Monstrous because it charts the development of that planet from one story to the next with far more clarity than it does it's characters. I take back what I said in my previous review about the opening story being irrelevant, this is very much a three part story where part one introduces Keska and it's local problems, part two visits the planet years later under Dalek rule and part three charts how the planet takes its place in the great Time War. It's bloated and full clichés along the way but the journey has at least been going somewhere. The Heart of the Battle has some enormous ideas at its heart, ones that have the potential make some very exciting storytelling. However those ideas need to be explored through its characters and in engaging scenarios...what happens here is the big concepts are dumped on the audience in one enormous gulp of exposition. You want to know what has been happening in this box set? Well prepare yourself for fifteen minutes worth of explanation! The answers should emerge naturally from the narrative, rather than being dumped upon it. This whole set could have done with an edit to ensure that the conclusion felt like a natural extension of events rather than a lecture explaining what it has all been about. I really like Nick Briggs as both a writer and a person, I think he has talent and he is a very good ambassador for Big Finish. Lately though his efforts are being hampered by the fact that his finite talent is being stretched in too many directions. His fingers are in so many pies that only his thumbs are left to use the keyboard. Creatures of Beauty is what I always come back to because it is the yardstick to which I compare the rest of his work to. That was his ultimate achievement and it came at a time when he wasn't encumbered with stories to write for two dozen ranges. Only the Monstrous isn't a disaster. If you have never heard a Big Finish story before this would be an exciting place to jump in because of Hurt's involvement and the added thrill of the Time War. But for seasoned audio listeners there is little that is new here...and that is rather what was promised. I can tell you without doubt that the second set will be more enticing - Dorney, Fitton and a new writer. Tasty. The Heart of the Battle gets a thumbs up for a truly mind-boggling and devastating Dalek plan. It's just a shame it couldn't be less of a lecture and more of a drama. A point removed for the pointless suggested murder at the climax. She'll be back. Just call her Molly O'Sullivan the Second: 5/10


text:
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Compared to the rest of the Metropolitan Los Angeles commercial real estate market, the Dana Point submarket has more expensive rates for rent, and you may need to make a great offer to be in this hot submarket.
In the last 90 days, 34 commercial real estate listings have been posted online, which means new listings might be leasing quickly. You'll want to be prepared to tour spaces as quick as possible. There aren't many listings below 2,000 square feet available, so finding a space for a small business might be tough.
The standard size for office space in this area is around 2,800 sqft. Most properties in this region are around 2 floors. Most buildings in this area were built close to 1984 and are Class C.
Real time and historical data on commercial real estate listings in Dana Point.
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Good Question: Nice Trunks
Louise sent us an email: Instead of putting dressers in my daughter's room (she's 7), I was thinking of putting a trunk or two in her room. She could use them as clothing storage and as a table top. Do you have any cute trunks you could recommend?
Great idea and good question, Louise!
We love the idea of using a trunk as storage and a side or coffee table. It's definitely a way of getting more bang for your buck and utilizing all of your space wisely.
If you're looking for a fabric trunk, we like Pottery Barn's Lewis Trunk, $349. It comes in a nice variety of fabric choices.
Target's wood storage trunk is a nice classic, that is not a bad find for the price. It's on sale for $59.99.
Do you have a trunk you can recommend to Louise?


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Reuters Staff
1 Min Read
Sept 29 (Reuters) - Abbott India Ltd
* Approved appointment of Ambati Venu as additional director on the board and as the managing director Source text: bit.ly/2cDtAuw Further company coverage: (Bengaluru newsroom)
All quotes delayed a minimum of 15 minutes. See here for a complete list of exchanges and delays.
© 2017 Reuters. All Rights Reserved.


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18900 Delaware Street UNIT 218, Huntington Beach CA, 92648 (Residential)
Sold Price:
1 bed , 1 Full baths
561 sqft
Status: Sold
Last Updated: 11/21/2017 9:49 AM
Description for 18900 Delaware Street UNIT 218 Huntington Beach, CA 92648
Unit is located on the second floor with view from living room and bedroom of the common area facing North. Refrigerator is included and is 4 years old. All other appliances plus the heater and a/c unit are 8 years old
Sold Price:
Sold Date:09/28/2015
1 Full
561 (approx)
Unit is located on the second floor with view from living room and bedroom of the common area facing North. Refrigerator is included and is 4 years old. All other appliances plus the heater and a/c unit are 8 years old
Listing Information
Electric Water Heater, Public Water Service
Sewer Connected, Sewer at Street
Electric service available
Room Information
Shower Only
Interior Features
Dishwasher, Electric Oven, Electric Range/Cooktop, Refrigerator, Carbon Monoxide Detector
Carpet Wall to Wall
Central Air, Gas
No Fireplace
Alarm -Smoke/Fire,
Elevator, Fire Sprinklers
Exterior / Lot Features
1 Parking Spaces, Handicap Parking, Assigned Parking Space, Uncovered
Balcony, Deck
No Pool
Sidewalks, Street Lights, Storm drains
Financial Considerations
Listing Price History
Huntington Beach Real Estate
Huntington Beach is located in California. Huntington Beach, California 92648 has a population of 201,899.
The median household income in Huntington Beach, California 92648 is $82,554. The median household income for the surrounding county is $75,998 compared to the national median of $53,482. The median age of people living in Huntington Beach 92648 is 40.9 years.
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Nuclear Science and Engineering Lab (NSEL)
Nuclear Science and Engineering Lab (NSEL) operates under the auspices of Institute for Critical Technology and Applied Science (ICTAS). NSEL is engaged in research and education in different application areas of nuclear science and engineering: power, medical, security and safeguards. NSEL is part of the Nuclear Engineering Program within the Mechanical Engineering Department.
BOOK: Download corrections (updated 2017) for my book on Monte Carlo Methods for Particle Transport
To learn about recent activities, visit News and Events.
NEW BOOK: Monte Carlo Methods for Particle Transport - Dr. Alireza Haghighat RAPID: Real-time Analysis for spent fuel Pool In-situ Detection PENTRAN and TITAN: Deterministic Methods for Particle Transport AIMS: Active Interrogation for Monitoring Special nuclear materials Automated variance-reduction techniques and electron transport using MCNP INSPCT-S: Fission Matrix approach for Inspection of Spent Fuel Pools Gamma Ray Modeling Penn State Breazeale Reactor Core TITAN NCAT Voxel Phantom model TITAN HTTR Fuel Assembly Model TITAN Iterative Image Reconstruction 5 Iters. 10 Iters. 20 Iters. 40 Iters. Reference


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Yachting Australia seeks reconsideration for 2012
by Phil Jones, Yachting Australia on 13 Mar 2008
Will the Yachting Australia and other submissions to ISAF be eoungh to get the Tornado over the 2012 Olympic startline? Richard Gladwell
Yachting Australia have released the submission sent to the International sailing Federation (ISAF) supporting the re-opening of the November 2007 decision on the Events chosen for the 2012 Olympic Games.
The announcement by Yachting Australia follows a similar course to that adopted by the Royal Yachting Association.
There is now the situation were the two most successful nations at the 2007 Olympics in Qingdao are now urging ISAF to reconsider the Event selection for the 2012 Olympic Regatta. Between them Australia and Great Britain won Gold medals in seven of the 11 events contested in Qingdao in the Olympic format regatta (only one entry per nation permitted).
To date, ISAF has been batting away criticism of its handling of the Events affair, however with the closing date of 15 March looming for submissions. other major yachting nations are believed to be joining the fray for a re-opening either at the May Mid-Year Meeting or the November Annual meeting.
In a covering letter to ISAF Secretary General Jerome Pels, Yachting Australia CEO, Phil Jones said:
Dear Jerome,
Please find attached our submission regarding the 2012 Olympic Events. We request
that this submission be considered urgent under ISAF Regulation 1.6(b) and placed
on the agenda for the Council Meeting at the Mid Year Meetings in Qingdao in May.
Yachting Australia acknowledges that it is for the Executive Committee to determine
whether this and similar submissions are urgent. We note from the minutes of the
Executive Committee held on 15 February 2008 that this has been considered. We
further note that no submissions had been received at that time. We trust that now this
and other submissions have been received, and some of the substantive issues have
been highlighted, the Executive Committee will reconsider its position.
We understand that the Mid Year Meetings are intended mainly to deal with
administrative matters and an item of this nature would normally be dealt with at the
Annual Meetings. We support this general approach. Most decisions made can wait
until the following year to be reconsidered. It is for this reason that ISAF is not flooded
with submissions to the Mid Year Meeting to reopen decisions made at the previous
Annual Meetings.
However we strongly believe this matter to be an exception that should be dealt with
as urgent.
There are a number items that flow from the decision on Events that can only be
addressed once this matter is resolved. These include the selection of Equipment for
the various Events. For this reason alone, we consider the matter urgent.
If the Executive Committee prevents the matter being addressed by Council in May,
MNAs will have no alternative but to make submissions on the matter to the Annual
Meetings in November. Our concern is that to delay consideration of this and other
similar submissions until November will mean that, if Council agrees to reopen the
matter of the Events, the Events and Equipment will have to be decided at one
meeting. This will create uncertainty and confusion and will make the management of
the Annual Meetings very difficult.
There seems to be a view that for the Executive Committee to put this matter to
Council and to allow them to address it is in some way disrespectful of the decision
that Council made in November. We take the opposite view. The ISAF Council made
the original decision. ISAF Regulations make provision for decisions made under
16.1.1(b) to be reconsidered. ISAF has in place a democratic process that allows the
Council to address exactly this matter.
Yachting Australia has the greatest respect for the ISAF Council and its members. We
are aware that this is a very difficult matter. We are also aware however of the
significant concern within the sailing community, and amongst some Council
members, over both the voting process adopted by Council last November when
making the decision and the impact of this decision for the future of sailing in the
Olympic Games. For Council to consider reopening the matter is entirely appropriate.
The Council is scheduled to meet May. This provides the perfect opportunity for this
and other similar submissions to be discussed. We urge that the matter be on the
agenda. If there is not sufficient support around the Council table for reopening, it fails
at that point.
I look forward to hearing from you.
The full submission released by Yachting Australia is as follows:
Selection of Events for the 2012 Olympic Sailing Competition
A submission from Yachting Australia
That the decision recorded at item 13(d), Selection of Events for the 2012 Olympic
Sailing Competition, of the Minutes of the ISAF Council Meeting held on 8/9 November
2008 in Estoril, Portugal, based on a motion from the floor by Charley Cook (USA), be
reconsidered by a meeting of the ISAF Council at the earliest available opportunity.
If, in reconsidering this motion, the ISAF Council decides that a revote is appropriate,
there should be either a run off vote between:
a) Multihull (Open) and the Keelboat (Men)
b) Two Person Dinghy High Performance (Women) and Keelboat Match (Women)
or the list of Events for the 2012 Olympic Regatta, as decided in Estoril in November
2007, should be set aside and a new vote taken, using the voting process as
recommended by the Events Committee without the change that resulted from the
motion made by Charley Cook.
1. In May 2007 the Events Committee considered recommendations from its Event
Strategy Working Party regarding the most suitable voting process for determining the
2012 Events. The recommendations were further refined by Working Party before being
adopted by the Events Committee in November. The document outlining the voting
process was widely discussed and available during the Annual Meetings.
2. The recommended voting process was based on a number of fundamental principles,
the key one being that no Event should be eliminated until it had failed to receive
50% of the valid vote cast in the first 'slate' ballot or any subsequent run-off
ballot. The process ensured that when no events had received fewer than 50% of the
votes in the first round, the two Events with the lowest number of votes were subject to
run-off vote, and the one with fewer than 50% of the vote was then eliminated. This
process was used when the votes were taken at both the Women's Committee and the
Events Committee during the Annual Meetings.
3. Council considered a number of submissions from the floor to amend the voting process
recommended by the Events Committee. Most had no impact on the final outcome.
However Charley Cook (USA) proposed the removal of the run-off ballot. The minutes
record that his argument was based solely on the fact that 'the voting process could be
simplified and be clearer for the sailing public to understand'. It was on this basis that he
moved his motion. This was seconded and was accepted by the Council.
4. During the discussion on the motion, it was noted that the change being proposed would
have made no difference to the outcome of the vote taken at the Events Committee.
Indeed, it would not have done. However, this appears to have led some Council
members to wrongly believe that the change proposed could not have an effect on the
outcome of the Council vote. In fact, it may well have had an effect.
5. There was no suggestion at the time from the proposer that the motion would change the
fundamental principles outlined above. Clearly it did. Despite receiving more than 50%
of the votes of Council, both the Multihull (Open) and the 2 Person Dinghy High
Performance (Women), have been removed from the Events for the 2012 Olympic
6. Had the original process been adopted without amendment, there woul
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MyEssayWriting.com - Essay Writing Services.
English Teaching Professional is a quarterly magazine which is packed with classroom activities and teaching tips for every English language teacher.Every issue includes regular sections such as:
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Here are five players to watch in Sunday's Champaign (Ill.) Nike Football Training Camp.
Alec James (Brookfield, Wisc./Brookfield Central)
Playing in Wisconsin, James is not on many radars. The 6-foot-4, 225-pound defensive end should be, though. Clemson, Michigan State, Notre Dame and Wisconsin have all offered and are his four leaders. Because he's a pure speed rusher, it will be interesting to see how James does in one-on-one drills against bigger linemen if he cannot line up too wide.
Brandon Hines (Mequon, Wis./Homestead)
One of the region's top defensive tackles in the Class of 2012, Hines is also one of the strongest. In March, Hines said he was benching 305 pounds "easy." On Sunday, Hines will go against a number of other linemen who also can bench 305 pounds without breaking much of a sweat. Though Hines does not have an offer, a host of Big Ten programs checked in on him during the spring evaluation period. He should have his first offer soon, as he makes the rounds at a host of camps and can show what he can do without the club he was forced to play with his sophomore season.


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about 1 1/2" across, and about 6" long -- old time powder horn -- wooden end held in with tiny brads with a nail in center to hook a strap to. carved end - has some pretty good age to it -- free shipping. . ................................................................ ............................................................. [ [ [NOTE -- ITEMS OVER $100 IN VALUE WILL BE SENT INSURED WITH DELIVERY CONFIRMATION - NO EXCEPTIONS -- THIS PROTECTS YOU AND ME -- I'M TIRED OF PEOPLE COMPLAINING THAT THEY DIDN'T GET THEIR PACKAGE -- STUFF HAPPENS IN THE MAILS AND I DON'T WANT TO HAVE TO PAY FOR IT -- IF IT'S A BIG TICKET ITEM EXPECT IT [ [ - again a whole bunch of different items -- some a bunch of; others only one of a kind -- haven't gotten closer to cleaning up the house -- hope this is a start - as always, ASK ANY QUESTIONS BEFORE BIDDING -- i know i'm a lousy photographer --- need better photo -- let me know and i'll try - i will try to describe as accurately as i can -- need more info --- send an email - i'll answer any( well almost any) questions -- when possible i combine auctions for reduced shipping - i will describe any and all flaws and problems that i find - -IF YOU HAVE A PROBLEM EMAIL ME = ask questions if you are concerned about any item -- good luck and happy biding -- I will ship only to USA -- i accept other forms ... read more


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Discussion in '1911 Forums' started by 123me, Jul 25, 2014.
So, I've got a Taurus PT1911 in 9mm on the way. Should I plan on buying AMMO or ASPIRIN ?
If you are worried about it then why buy it? I would buy ammo and assume it will be O.K.
You could certainly sell it to Fipper before you shoot it. He thinks they are right up there with the Wilsons and Baers.
Neither,......get a box of tampons.
When have I ever said anything like that??
Buy some aspirin and save the ammo until after you buy some parts for it.
The frame and slide will be ok to keep.
Best of luck.
That's the only thing worth a damn on the Taurus. Of course, they managed to ruin the frame with those scratches they try to pass off as checkering.
Taurus, Keltec, and some Rugers don't get much if any respect here on GT. If it's reliable for you, enjoy. If not, I've heard that they improved their support by adding more people on the phones and processing returns so hopefully the warranty repair turn around time is greatly reduced.
Sent from my Nexus 7 using Ohub Campfire mobile app
I agree about Taurus and KelTec, but I think Ruger gets plenty of respect on GT. Sure there are haters, but that goes for any brand.
I'm actually not a Taurus hater. I've got a few Taurus revolvers that have been great and I also have a PT92 that I like. The 1911 leaves something to be desired, however. I think its the worst current production 1911 on the market.
I said "some Rugers" and I was referring to the LC9 and SR9C since many don't like the extra safety features.
I'd love to buy a Taurus, but the QC is just too hit or miss. The last two I shot, a PT1911 and a Raging Bull both had problems.
Flipper is going to flip when he sees that!
Buy ammo, and lots of it. Ive got well over 1,000 rounds thru my PT 1911 in .45 ACP with zero problems. Only thing Ive done to it is switch that anal ambi thumb safety that kept coming on while shooting with a Wilson Combat single side thumb safety. Cant find any other negatives about the gun, except the finish is of poor quality and will wear off soon.
now that's funny
I bought a Taurus 1911 in 45acp and assumed it would be a potential box of problems.. but mine has nice sights and works very well. My only issue is that the pistol is very loose shooting. The finish is not nearly as nice as an average bbq grill either.
Check the screw that holds the rear sight down. Its a tiny little set screw set so deep in the sight that you need a good light and a magnifying glass to see it. Mine came loose. It started shooting all over the place, and when I found the rear sight was loose, and I tightened it up, it started shooting fine again.
A friend of mine bought one (one of the "match" models) NIB for a good price and after 400 rounds the extractor broke. Hope you have much better luck with yours OP!
Update- Runs GREAT looks like crap!


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2013 Ford EscapeEnlarge Photo
Why it's on this list: Four recalls announced in 2012
- Overheat may cause fluid leaks resulting in fire (12V551000, 80,057 Escapes)
- Cup (freeze) plug dislodging (12V431000, 6,146 Escapes)
- Engine compartment fuel line (12V336000, 9,320 Escapes)
- Pedal package clearance variation (12V319000, 8,266 Escapes)
Manufacturer's hotline: 888-327-4236
The 2013 Ford Escape, which hit the market last spring, is an especially important product for Ford—as the previous Escape had been a longtime best-seller among compact crossovers. Yet the new Escape got an uneasy start out the gate, with an embarrassing total of four different recalls by year end covering safety-related issues—three of them potentially leading to fire, and/or rather catastrophic engine failure. It's true, however, that considering the high volumes this vehicle is produced in, these recalls covered a relatively small portion of Escapes made; and they're better caught early than when a significant number of these show up on the used-car market.


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Seagull R21554T
- Pet Friendly
- Pool Table
- WiFi
- Pillows & Blankets
- Coffee Maker
- Toaster
- Blender
- Dishes, Utensils, & Cookware
- Dishwasher
- Microwave
- Iron & Ironing Board
- Stereo with CD Player
- Telephone
- TV & DVD Player
- Central Heat & Air Conditioning
- Washer & Dryer
- Outdoor Furniture
- Outdoor Shower
- Grill
- Beach Chairs (5 per home)
Home Details
|Unit Number:
|Seagull Beach
|Check in Day:
|Check-in Office:
|Distance to Beach:
|View Map
|5 (1K, 2Q, 1T, 1BS, 2Duo)
|Master Bedrooms:
|4 Full
|4x4 Required
Twiddy Traditional Home - bed and bath linens not provided. Accessible by 4x4 only. This semi-oceanfront home in the beautiful 4x4 area offers ocean views and a great getaway spot for the whole family, including your dog! Approximately 2.6 miles up the beach.
Main House
|Level 1
|BR/rec room (2 built-in Duos) with pool table; full bath; laundry.
|Level 2
|MBR (K); three BRs (Q, Q, T/BS); full bath; covered decks.
|Level 3
|Great room; kitchen with breakfast bar; full bath; open decks.
Dogs welcome (2 max., no cats). No additional fee or deposit required.
Click here for satellite parking information.
Ratings & Reviews
- Lori 7/29/2017
- Gay 6/24/2017
- Susan 8/20/2016
Seagull---fabulous location, great house for our family. Loved our stay and have booked a return next summer.
- Gay 8/6/2016
- Danny 7/9/2016
Questions & Answers
Is there parking at the house and for how many cars? Thank you
Laura, there is parking for approx. 4 vehicles provided at the home.
- Answered by Twiddy on 12/28/2016
Hello- Is early check-in available for this property, and if so, what is the additional cost?
Bard, this currently offers the early check-in option, however, availability varies by check-in day. To check availability for your week, please contact the office at 1-800-489-4339 or [email protected]
- Answered by Twiddy on 7/8/2016
How many TVs are in this home and which rooms? Thanks!
Hi Barb! This home provides 2 TVs. One is located in the main, top level living room area and the other is located in the ground floor rec room area.
- Answered by Twiddy on 2/8/2016
Are the bottom bunk beds twins or full size? Just wondering what size sheets we need to bring
Darcy, R21554T currently provides 1 king, 2 queens, 1 twin, 1 bunk set, and 1 duo. The bunk set has a twin on top and a twin on bottom. The duo bunk has a full size bed on bottom and a twin size bed on top.
- Answered by Twiddy on 7/16/2015
Does the kitchen have a blender and crock pot?
Thank you for your inquiry, Donny! The home will provide a blender as that is a standard amenity in every home. However, crock pots/slow cookers are considered specialty items and are not advertised or guaranteed amenities. We suggest you bringing one from home if you're going to be needing one during your stay. To view a complete list of standard amenities that are offered in all of our homes, please see: https://www.twiddy.com/pre-arrival-info.aspx
- Answered by Twiddy on 7/15/2015
Does home have any beach items such as chairs, toys,etc. & also have an outdoor shower?
Mary Ann, every Twiddy home provides 5 beach chairs. Additional beach equipment such as umbrellas, beach toys, and additional chairs are not a standard amenities. If you would like to rent additional beach equipment, Just for the Beach, Moneysworth, and Ocean Atlantic Rentals all provide this service. For contact information, please visit www.twiddy.com/activities/services.aspx. An outside shower is a standard amenity for all Twiddy homes. To view our list of standard amenities, please visit www.twiddy.com/abbreviations-definitions.aspx#stfurneq.
- Answered by Twiddy on 6/25/2015
If bed & bath linens are not supplied does that include bed pillows also?
Thank you for your inquiry, Mary Ann! R21554T does not provide bed linens or bath towels, however, pillows, blankets, and comforters are supplied.
- Answered by Twiddy on 1/16/2015
can we drive atv's on the beach
Great question, Danny! Unfortunately, ATV's are strictly prohibited in the 4x4 area. For additional information, please visit www.twiddy.com/faq.aspx#misc. Please let us know if you have any additional questions.
- Answered by Twiddy on 7/3/2014
How big is the grill? Could you provide any pictures of it?
Mark, the grill at this home is charcoal, but we do not have a record of the size, or any other photos that would include the grill!
- Answered by Twiddy on 7/18/2013


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Mondovi High School Alumni
Mondovi, Wisconsin (WI)
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Renee Getty (Patton), '83
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Judy Johnson (Weishaar-rudin), '63
Rod Moats, '77
Margie Folkedahl (Larsen), '68
Lori Loomis (Whelan), '89
Kim Kim Peichel (Peichel), '44
Lori Nelson, '55
Patrick Rothbauer, '79
Maria Lane, '90
Michael Rothbauer, '81
Rich Bentley, '60
Melissa Sganga, '94
Lynn Anderson, '74
Fred Johnson, '80
Margie Folkedahl (Larsen), '68
Bill Taylor, '08
Jeff Hill, '79
Deneise Yarrington, '87
So you caught up with some of our Buffaloes at this years class reunion, don't lose touch again until the next get together! Take advantage of the upcoming holiday season and reach out to them. It can even be something as simple as wishing them "Happy Holidays"! Use the tools on this page to search for classmates that you have lost touch with over the years!
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, (PressExposure) November 13, 2008 -- There is some good news for ardent art lovers, and that is that there is a new online store on the block that is selling Van Gogh Replicas.
The new store, Vangoghstudio.com is an out and out website dedicated to reproducing and selling Vincent Van Gogh's Paintings.
There are thirty different Van Gogh Replicas that are available in this site.
The site claims to have to have made all the thirty paintings with utmost care and research and also at the same time, they have made sure that they have caught all the nuances of the painting, so much so that it is difficult to tell it apart from the original.
They have only made thirty reproductions and the reason cited for this was that they wanted to have a quality control over the paintings that they offer.
The spokesperson from Vangoghstudio.com says "Not everyone can afford an original Vincent Van Gogh Painting, so it struck us that this was in a way helping promote the work of Van Gogh even more."
The paintings over at Vangoghstudio.com have been approved by authorities that matter and there have been no issues as of now.
It would be a great help for art collectors, as now they can get original like Van Gogh Paintings for real cheap.
There are many stores that are into replicating the works of Van Gogh but it still remains a question mark about who will remain at the end of the race.
The future of Vangoghstudio.com lies in the quality of its paintings.
You can browse and buy Van Gogh paintings at- http://www.vangoghstudio.com/


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Histamine increases microvascular permeability by creating small transitory (100–400 nm) gaps between adjacent endothelial cells at sites of vascular endothelial (VE)-cadherin-based adhesion. We examined the effects of histamine on the proteins within the VE-cadherin-based adherens junction in primary human umbilical vein endothelial cells. VE-cadherin is linked not only by β- and α-catenin to cortical actin but also by γ-catenin to the intermediate filament vimentin. In mature human umbilical vein cultures, the VE-cadherin immunoprecipitate contained equivalent amounts of α- and β-catenin, 130% as much β- as γ-catenin, and 50% as much actin as vimentin. Within 60 s, histamine decreased the fraction of VE-cadherin in the insoluble portion of the cell lysate by 35 ± 1.5%. At the same time, histamine decreased the amount of vimentin that immunoprecipitated with VE-cadherin by 50 ± 6%. Histamine did not affect the amount of actin or the amount of α-, β-, or γ-catenin that immunoprecipitated with VE-cadherin. Within 60 s, histamine simulated a doubling in the phosphorylation of VE-cadherin and β- and γ-catenin. The VE-cadherin immunoprecipitate contained kinase activity that phosphorylated VE-cadherin and γ-catenin in vitro.
- vascular endothelial cadherin
- adhesion
histamine increases microvascular permeability in postcapillary venules (37). Early studies from Majno and Palade (21) indicated that one of the mechanisms by which histamine increased venular permeability was the transient development of gaps between adjacent endothelial cells. McDonald and colleagues (22) quantitated these changes and measured gaps of 100–400 nm, which have a half-life of ∼1.9 min. Restriction of the edemagenic effects of histamine to venules may reflect the high concentration of histamine receptors in venules relative to other vascular segments (15).
Activation of the histamine H1 receptor caused similar small gaps to develop between adjacent human umbilical vein endothelial (HUVE) cells (5, 6, 24). These decreases in cell-cell adhesion occurred in the absence of any detectable increases in tension within the cells (23). The absence of an increase in tension suggested that histamine might cause gaps to form by altering cell adhesion, unbalancing the forces resisting the resting tension within the cells. Histamine could affect adhesion at sites of apposition between adjacent cells or at sites of apposition of the ventral surface of the cells to their matrix. Histamine altered adhesion at sites between adjacent cells (24).
In endothelial cells, cadherin-5, or vascular endothelial (VE)-cadherin, is an important constituent of cell-cell adhesion (7). We hypothesized that histamine acted at sites of VE-cadherin-based adhesion. ECV304 cells respond to histamine with an increase in cell calcium, similar to endothelial cells (35). ECV304 cells do not express VE-cadherin, and histamine did not change the adhesion of control ECV304 cells. However, histamine decreased cell-cell adhesion of ECV304 cells transfected with VE-cadherin by 60%. The decrease in adhesion followed a time course similar to that observed in HUVE cells, which is similar to the kinetics of gap formation and resolution in situ (22, 35). Hence, histamine alters VE-cadherin based cell-cell adhesion.
Changes in adherens junction adhesion are frequently associated with changes in phosphorylation of elements of the adherens junction complex (8). Assessing phosphorylation of the VE-cadherin complex is complicated by the fact that tyrosine phosphorylation of elements of the complex decreases with maturation of the cells (19). Several authors have reported that agonists that alter endothelial adhesion initiate increases in tyrosine phosphorylation of VE-cadherin, p120, and β- and γ-catenin in endothelial cells (1, 9,32). However, none of these authors could detect increases in phosphorylation of VE-cadherin or the catenins after exposure to the agonists without pretreating the cells with inhibitors of tyrosine phosphatases. More recent reports demonstrate that the phosphatase inhibitors, themselves, increase phosphorylation and alter adhesion. Hence, the physiological inference of the observations made in the presence of the inhibitors is uncertain (12, 36).
Andriopoulou et al. (1) reported that histamine decreased the amount of VE-cadherin in the detergent-insoluble fraction of the cell lysate, implying a decrease in VE-cadherin association with the cytoskeleton. However, no one has detected a change in the amount of any of the proteins that are associated with VE-cadherin when it is immunoprecipitated from cells exposed to thrombin, histamine, or vascular endothelial growth factor (VEGF) (1, 9, 32). Hence, although there is general agreement that VE-cadherin-based adhesion is altered, there are not currently any data to explain how it is altered.
The classic type I cadherins, E-, N-, and P-cadherin, are linked to cortical actin by β- and α-catenin (2). VE-cadherin is a type II cadherin, and it is linked not only by β- and α-catenin to cortical actin but also by γ-catenin and desmoplakin to the intermediate filament protein vimentin (18, 33). The importance of the linkage to vimentin is highlighted by the fact that desmoplakin-deficient mice do not survive embryogenesis. They have a defective microvasculature and resemble VE-cadherin-deficient mice (10, 11). When endothelial cell cultures are wounded, VE-cadherin complexes with γ-catenin respond to the wounding and facilitate cell movement much more than β-catenin-containing VE-cadherin complexes (20). This raises the possibility that γ-catenin-based VE-cadherin complexes respond more to agonists that affect cadherin-based adhesion.
In the experiments reported in this manuscript we used 32P labeling to assess changes in phosphorylation of elements of the VE-cadherin complex after exposing HUVE cells to histamine, and we did not pretreat the cells with phosphatase inhibitors. We used [35S]methionine labeling to determine changes in protein binding within the VE-cadherin complex. We used only mature cultures (≥4 days postconfluence), and we examined these events 60 s after initiating exposure to histamine, a physiologically relevant time when adhesion has reached its nadir (23, 24). We found that histamine stimulates an increase in phosphorylation of VE-cadherin, β-catenin, γ-catenin, and vimentin within 60 s. Consistent with the observations of Andriopoulou et al. (1), we also found that histamine decreased the amount of VE-cadherin that was in the detergent-insoluble, cytoskeleton-associated fraction of the cell lysate. We did not detect a change in the amount of any of the catenins or actin present within the VE-cadherin immunoprecipitate, but we did detect a 50% decrease in the amount of vimentin that was present. These observations suggest that histamine primarily affects the interaction of VE-cadherin with the vimentin cytoskeleton.
Fibronectin was from Collaborative Research (Bedford, MA). Tissue culture media and serum were from the Tissue Culture Core, University of Iowa. Antibody to VE-cadherin (mouse IgG, monoclonal, clone 55-7H1) was from PharMingen. Antibody to E-cadherin was clone rr1 from the Hybridoma Facility. Antibodies to β-catenin and γ-catenin were rabbit polyclonals directed to the epitopes described by Hinck et al. (16). Antiphosphotyrosine was from Upstate Biotechnology (Lake Placid, NY). Secondary antibodies were from Amersham. [32P]ATP and [γ-32P]ATP were from New England Nuclear.
Cell cultures.
Cultured HUVE cells were prepared by collagenase treatment of freshly obtained human umbilical veins and cultured. Cultures were identified as endothelial by their characteristic uniform morphology, uptake of acetylated low-density lipoprotein, and by indirect immunofluorescent staining for factor VIII (35).
Immunoprecipitation and blotting.
For immunoprecipitations, cells were grown on Transwell filters for 5–7 days. In some experiments cells were placed in methionine-depleted Selectamine (DuPont NEN) media with 1 mCi per Transwell of [35S]methionine (DuPont NEN) 36 h before the experiment. In other experiments, cells were placed in phosphate-free media, and 0.8 mCi of 32P as inorganic phosphate was added to the media 3 h before the experiment. Cells were washed and then exposed to histamine for the indicated times. After exposure, cells were lysed and immunoprecipitated as described by Hinck et al. (16). Cells were immediately lysed in 4°C CSK buffer [50 mM NaCl, 10 mM PIPES, pH 6.8, 3 mM MgCl2, 1% Triton X-100, 300 mM sucrose, 5 mg/ml phenylmethylsulfonyl fluoride (PMSF), 5 μg/ml aprotinin, 1 mg/ml leupeptin, 5 μg/ml pepstatin A, 4 mM Na orthovanadate, 10 mM NaF, and 10 mM NaP2O7] and kept on ice for 20 min. The cells were scraped from the filter and added to the CSK buffer, which was centrifuged at 12,400 g for 10 min. Soluble material was transferred to a separate tube for immunoprecipitation.
The soluble fraction was precleared with 30 μl of Sepharose (Sigma CL 4B200) and 5 μl of gamma bind (Pharmacia 17-0886-01) preincubated with 250 μg of mouse IgG in a total volume of 100 μl of Tris-buffered saline [TBS (10 mM Tris, pH 7.5, 150 mM NaCl, 2 mM MgCl2, 2 mM CaCl2, 1 mM PMSF, 2 μg/ml aprotinin, 15 μg/ml leupeptin, and 360 μM 1.10-phenanthroline)] for 2 h at 4°C. The beads were pelleted, and the precleared solutions were then incubated with 30 μl of Sepharose and 5 μl of gamma bind that had been preincubated in 500 μl of anti-cadherin-5 (VE-cadherin) media for 2 h at 27°C and then rinsed in 500 μl of TBS. Samples were rotated overnight with anti-cadherin-5-Sepharose-gamma bind at 4°C. Beads were pelleted at 12,400 g for 2 min at 4°C. Beads were then resuspended in 1 ml of high-stringency buffer (15 mM Tris, pH 7.5, 5 mM EDTA, 2.5 mM EGTA, 1% Triton X-100, 1% Na-deoxycholate, 0.1% SDS, 120 mM NaCl, and 25 mM KCl) underlain with 100 μl of 10% sucrose and centrifuged at 12,400 g for 2 min at 4°C (16). The pellet was then washed in high-salt buffer (15 mM Tris, 5 mM EDTA, 2.5 mM EGTA, 1% Triton X-100, 1% Na-deoxycholate, 0.1% SDS, and 1 M NaCl) followed by low-salt buffer (15 mM Tris, pH 7.5, and 5 mM EDTA).
For a selected set of experiments, the cells were preexposed to peroxide and pervanadate for 7 min before being exposed to histamine as described by Andriopoulou et al. (1). These experiments were used to determine the effects of peroxide and pervanadate, alone, on phosphorylation, and they confirmed the observations of Garcia et al. (12). The data from these experiments are not presented in the text.
Immunoprecipitated proteins were separated on 8% polyacrylamide gels. The proteins in the gels were transferred to polyvinylidene difluoride (PVDF) membranes. Autoradiograms were generated from the [35S]methionine-labeled transfers and quantitated by densitometry. Proteins were identified by immunoblotting, the same transfers with the indicated primary and secondary antibodies. Blots were quantitated by densitometry. In assessing the effects of histamine on the 35S mass of proteins immunoprecipitated with VE-cadherin, we normalized the mass of individual proteins (as assessed by the 35S signal) to the number of methionines and then divided by the mass of VE-cadherin immunoprecipitated in the same sample, so that each sample was normalized to itself and to methionine content.
In assessing the changes in phosphorylation, we quantitated the32P activity associated with individual proteins on the transfers by densitometry of an autoradiogram developed from the transfer. Again, proteins were identified by blotting the transfers. The mass of VE-cadherin in each transfer was quantitated using phosphorimaging (Molecular Dynamics) of a Western blot for VE-cadherin. The 32P activity for each protein in the sample was normalized to the mass of VE-cadherin in the sample estimated from this blot. This ensured that unequal loading did not contribute to estimating changes in phosphorylation.
Kinase assays.
Soluble and in-gel kinase assays were conducted as described by Raingeaud et al. (26) and Sette at al. (28), respectively.
Briefly, for the soluble kinase assay, immunoprecipitates were prepared as above but washed 2× in kinase buffer (20 mM MgCl2, 25 mM HEPES, 20 mM β-glycerophosphate, 20 mM p-nitrophenyl phosphate, 0.1 mM NaVO4, 2 mM dithiothreitol, 1.25 mM EGTA, and 1.15 mM CaCl2) after the low-salt wash. Individual samples were then resuspended in 20 μl of kinase buffer with 20 μM ATP, 5 μCi [γ-32P]ATP, and 10 μg myelin basic protein (MBP). Samples were rotated for 30 min at 27°C, and the reaction was terminated with 50 μl of 2× SDS sample buffer. Samples were heated to 95°C for 5 min and centrifuged to pellet the beads, and proteins in the supernate were separated on polyacrylamide gels. Gels were transferred to PVDF membranes, which were then used for autoradiography and Western blotting.
For the in-gel assay, immunoprecipitates were prepared as described above. After the low-salt wash, beads were placed in SDS sample buffer and boiled for 5 min. The proteins released from the beads were separated in a thin 6% polyacrylamide gel with 0.33 mg/ml MBP incorporated into the gel. The gel was washed and renatured overnight and then incubated with kinase buffer (25 mM HEPES, 10 mM MgCl2, 90 μM Na vanadate, and 5 mM β-mercaptoethanol), 26 μM ATP, and [γ-32P]ATP for 1 h. The reaction was stopped with 5% TCA in 1% disodium pyrophosphate. An autoradiogram was generated from the gel.
Statistical analysis.
Differences among groups were compared by analysis of variance, and individual group comparisons were done using a Tukey honestly significant difference test for post hoc comparisons of means. Differences were considered significant at the P < 0.05 level.
Proteins in the VE-cadherin immunoprecipitate.
The VE-cadherin immunoprecipitate from HUVE cells more than 4 days postconfluence contains α-, β-, γ-catenin, and consistent amounts of actin and vimentin (Fig. 1). We quantitated autoradiograms of the VE-cadherin immunoprecipitate from [35S]methionine-labeled cells for each of these proteins with densitometry and normalized the values for the amount of VE-cadherin in the immunoprecipitate and for the number of methionines in the respective protein. There were equivalent amounts of α- and β-catenin in the VE-cadherin immunoprecipitate (Fig.2). There was 68 ± 0.02% as much γ- as β-catenin, and there was 52 ± 0.05% as much actin as vimentin (Fig. 2).
We found very little p120 catenin in the VE-cadherin immunoprecipitate from mature cultures (Fig. 3). This is consistent with an earlier report (19).
Histamine decreased the cytoskeletal association of VE-cadherin and the amount of vimentin in the VE-cadherin immunoprecipitate.
Histamine decreases endothelial cell-cell adhesion at sites of VE-cadherin-based adhesion. To begin to understand how histamine alters VE-cadherin-based adhesion, we examined the effects of histamine on the association of VE-cadherin with the cytoskeleton and on the binding of proteins within the VE-cadherin immunoprecipitate.
Histamine decreased the fraction of VE-cadherin that was present in the detergent-insoluble (cytoskeletal associated) portion of the cell lysate by 35 ± 1.5% (Fig. 4). Histamine did not change the amount of α-, β-, or γ-catenin that immunoprecipitated with VE-cadherin (Fig.5, n = 33 control and 33 histamine-stimulated monolayers, six separate experiments). Histamine also did not change the amount of actin that immunoprecipitated with VE-cadherin [−16 ± 8%, P = not significant], but histamine decreased the amount of vimentin that immunoprecipitated with VE-cadherin by 50 ± 6% (Fig.6, n = 15 control and 15 histamine-stimulated monolayers, three separate experiments). The decrease in vimentin was evident in both the autoradiograms from35S-labeled cells and when the immunoprecipitate was blotted for vimentin (Fig. 6).
Histamine increased phosphorylation of VE-cadherin, β-catenin, γ-catenin, and vimentin.
Histamine increased phosphorylation of VE-cadherin, β- and γ-catenin, and vimentin that immunoprecipitated with VE-cadherin. After 60 s, the 32P activity of VE-cadherin, β-catenin, γ-catenin, and vimentin that immunoprecipitated with the VE-cadherin increased by two to five times above control levels (Fig.7, n = 16 separate control and 16 histamine-stimulated monolayers). It was not possible to detect phosphorylation of the trace amount of VE-cadherin-associated p120, consistent with other reports (19). These increases in phosphorylation occurred without pretreatment of the cells with phosphatase inhibitors, indicating that they are physiological changes within the first 60 s of the cells' exposure to histamine. For each sample, the 32P activity in each of the proteins was normalized for the mass of VE-cadherin in the same sample. The increase in vimentin phosphorylation was evident even though there was ∼50% less vimentin present.
Blots of immunoprecipitate from control and histamine-stimulated cells not pretreated with peroxide and vanadate did not demonstrate a difference in tyrosine phosphorylation of the immunoprecipitated proteins, consistent with reports of others (data not shown) (1,12). When HUVE cells were pretreated with peroxide and vanadate, there was a significant increase in tyrosine phosphorylation of VE-cadherin in control cells, also consistent with reports from others (data not shown) (12).
Kinase activity in the cadherin-5 immunoprecipitate.
Because histamine increased phosphorylation of proteins in the VE-cadherin immunoprecipitate, we examined the VE-cadherin immunoprecipitate for kinase activity. An autoradiogram of a transfer of proteins separated on a PAGE gel after a soluble kinase assay demonstrated phosphorylation of MBP (Fig.8).
The identity of some of the phosphorylated bands other than MBP was investigated by blotting the transfers used for the autoradiograms for components of the VE-cadherin complex. One of the additional bands migrated at a molecular mass (MM) of ∼130 kDa, and this band labeled with antibody to VE-cadherin (Fig. 8). The larger of the other two bands migrated with an MM of 80–90 kDa, and it reacted with antibody to γ-catenin (Fig. 9). The identity of the bands that migrate just above MBP and at ∼60 kDa are unknown (see Fig. 8).
To begin to characterize the kinase activity, we examined the ability of proteins from the immunoprecipitate, separated on a 6% PAGE gel, to phosphorylate MBP that was incorporated into a gel. We found a dominant band of activity that migrated between the 46- and 66-kDa markers, a fainter band that migrated below the 97-kDa marker, and a very faint band below the 46-kDa marker (Fig. 10).
Adhesive interactions between cells are essential for morphogenesis and homeostasis. For both purposes the adhesive interactions must be dynamic (14). Cadherins are calcium-dependent intercellular adhesion molecules with a single transmembrane domain and five extracellular repeats that mediate calcium-dependent homophilic binding to adjacent cells. The cadherin cytoplasmic domain binds catenins and thereby mediates attachment to the cortical cytoskeleton (3, 8, 14, 16, 25, 27, 30, 34,38).
The response of endothelium to histamine is a rapid, dynamic loss and restoration of cadherin-based adhesion (35). In this study, we rigorously demonstrate that histamine stimulates phosphorylation of elements of the VE-cadherin complex. More importantly, we observed that histamine alters the link of VE-cadherin to the cytoskeleton within the linkage to the intermediate filament protein vimentin. At the same time, we detected no evidence that histamine alters the linkage of VE-cadherin to actin. An effect of histamine on VE-cadherin linkage to vimentin, but not actin, has significant implications for the mechanisms by which histamine alters VE-cadherin-based adhesion.
VE-cadherin is a type II cadherin, retaining some of the properties of classical type I cadherins but also demonstrating significant differences (2). As indicated in the Introduction, VE-cadherin is linked not only to cortical actin, but also to the intermediate filament vimentin (2, 4, 18, 33). Our data shed some light on the magnitude of these linkages.
The estimated molar ratio of α- and β-catenin in all (before and after histamine) the immunoprecipitates of VE-cadherin from HUVE cells was 1:1. This suggests that either very little α-catenin is linked to γ-catenin or β-catenin is linked to another protein in addition to α-catenin. If all of the β-catenin is linked to α-catenin, and none of the γ-catenin is linked to α-catenin, then almost all of the γ-catenin could be linked to vimentin, the linkage altered by histamine.
The VE-cadherin immunoprecipitates from mature HUVE cell cultures contained ∼70% as much γ-catenin as β-catenin. Lampugnani and colleagues (19) previously reported that immature HUVE cultures were rich in β-catenin, whereas mature cultures were enriched in γ-catenin. They estimated the relative amounts of the two catenins by immunoblotting rather than metabolic labeling, and their blot suggests even less β-catenin in mature cultures than do our metabolic labeling data. These differences may reflect differences between primary (ours) and passaged HUVE cells (theirs). When we examined passaged human dermal microvessel cells, they had 130% as much γ-catenin as β-catenin, indicating that the relative amounts of the proteins can vary among cell types (data not shown). Regardless, in both cells there is abundant potential for linking VE-cadherin to the intermediate filament vimentin.
The VE-cadherin immunoprecipitates also contained two lower MM bands, one at 58 kDa and one at 41 kDa. These bands blotted with antibodies to vimentin and actin, respectively. Because both vimentin and actin exist as multimeric proteins, it was not possible to make a good estimate of how many actin or vimentin molecules bound to a single VE-cadherin molecule. However, after normalizing for methionine and the amount of VE-cadherin in the same immunoprecipitate, we found about two times as much vimentin as actin, indicating that the linkage to the intermediate filament vimentin is very insignificant.
In mature endothelial cultures, Lampugnani et al. (19) found that very little p120 was bound to VE-cadherin, and it was never tyrosine phosphorylated. We also found only trace amounts of p120 that immunoprecipitated with VE-cadherin. The limited amount of p120 bound to VE-cadherin may relate to the recent observation that the new armadillo protein p0071 appears to compete with p120 for binding to the same juxtamembrane domain on VE-cadherin. Interestingly, p0071 also may link VE-cadherin to desmoplakin and vimentin (4).
We found that histamine decreased the amount of VE-cadherin that was present in the detergent-insoluble phase of the cell lysate by ∼40%. This is similar to the earlier observations of Andriopoulou et al. (1). We did not detect any changes in the amounts of α-, β-, or γ-catenin that immunoprecipitated with VE-cadherin. However, while examining blots from 35S-labeled cells exposed to histamine for 60 s, we consistently detected a large (∼50%) decrease in the band at 58 kDa, which blots with antibody to vimentin. Another band at 41 kDa, which comigrates with a band that blots for actin, did not change after exposing the cells to histamine.
Gallicano and colleagues (10, 11) have established an essential role for desmoplakin and the intermediate filament cytoskeleton in the developing microvasculature. Kowalczyk et al. (4, 18) has demonstrated important roles for γ-catenin (plakoglobin) and now p0071 in recruiting desmoplakin to the VE-cadherin complex. We had taken special care to preclear all the lysates with nonspecific mouse IgG bound to the same type of beads that were subsequently used for the VE-cadherin immunoprecipitation. Despite this, we initially had some concerns about the specificity of the vimentin in the VE-cadherin precipitates. However, the dramatic change in vimentin binding, but not in actin binding, after histamine could not be explained on the basis of nonspecific binding within the immunoprecipitation, and it provided new information indicating that the effects of histamine target the linkage of VE-cadherin to vimentin.
The observation that histamine primarily affects the link to the vimentin cytoskeleton is also consistent with an earlier observation. When mature endothelial cell cultures were wounded, γ-catenin (plakoglobin)-containing complexes near to, but not within, the wound were altered, but β-catenin-containing complexes were not (20). This would be consistent with cytokines preferentially acting on γ-catenin-based complexes.
We do not yet know how histamine alters the link between VE-cadherin and vimentin. Gaudry et al. (13) and Stappenbeck (29) have reported that tyrosine phosphorylation of γ-catenin (plakoglobin) reduces its ability to bind desmoplakin and that serine phosphorylation of desmoplakin reduces its ability to bind vimentin. In addition, the structure and polymerization of vimentin, itself, is affected by phosphorylation (17, 31). The new observations of Calkins et al. (4) that p0071 links VE-cadherin and desmoplakin offers additional potential mechanisms that might alter the link of VE-cadherin to vimentin.
Andriopoulou et al. (1) reported that histamine increased tyrosine phosphorylation of elements of the VE-cadherin complex in endothelial cells. Other authors reported similar effects of VEGF and thrombin (1, 9, 32). However, each of these studies found that it was not possible to detect changes in tyrosine phosphorylation unless the endothelial (20) cells were pretreated with pervanadate. More recently, Garcia et al. (12) found that similar treatment with pervanadate altered endothelial cell adhesion and increased phosphorylation of the same proteins. The results Garcia et al. raised questions about the physiological inference of the prior observations. Did the increase in tyrosine phosphorylation caused by the agonists detected in the presence of pervanadate represent a physiological response, or did it reflect results that would occur only with aberrant regulation? The observations by Wong et al. (36) that VEGF and histamine decreased, and not increased, phosphorylation of p120 in HUVE cells heightens this concern.
In our experiments, histamine increased phosphorylation of VE-cadherin and β- and γ-catenin in the VE-cadherin complex in HUVE cells. We measured phosphorylation as the incorporation of 32P into the proteins. We did not pretreat the cells with phosphatase inhibitors, and therefore these increases in phosphorylation represent a physiological response to histamine that occurs with intact phosphatase activity. As others have reported, we could not detect changes in phosphorylation of tyrosines in these proteins in cells not pretreated with tyrosine phosphatase inhibitors. The fact that we detected increased phosphorylation with 32P and not with antiphosphotyrosine suggests either that the 32P is more sensitive or that the phosphorylation is not on tyrosines.
We identified kinase activity in the VE-cadherin immunoprecipitate that phosphorylates VE-cadherin and γ-catenin. We do not yet know if this activity contributes to the effects of histamine on VE-cadherin- based adhesion.
In summary, histamine stimulates phosphorylation of elements of the VE-cadherin complex, and this can be detected without inhibiting phosphatases in the cells. We have confirmed that histamine causes VE-cadherin to move from the detergent-insoluble to the detergent-soluble fraction of the cell lysate. We have made the new observation that histamine affects the link between VE-cadherin and the vimentin cytoskeleton without causing a detectable change in the link between VE-cadherin and the actin cytoskeleton. These observations will help direct future investigations into how histamine alters VE-cadherin-based adhesion.
This work was supported by National Heart, Lung, and Blood Institute Grants HL-335450 and HL-63100.
Address for reprint requests and other correspondence: D. M. Shasby, Univ. of Iowa College of Medicine, Iowa City, IA 52242 (E-mail:michael-shasby{at}uiowa.edu).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
First published January 11, 2002;10.1152/ajplung.00329.2001
- Copyright © 2002 the American Physiological Society


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Click here for a complete list of the Washington Redskins' roster moves.
Biggest surprise: The decision to keep Chris Neild as a backup nose tackle and release Anthony Bryant. There was some chatter in the offseason about Bryant's possibly being promoted to starting nose tackle if the Redskins focused their offseason energies on defensive end. But once they signed Barry Cofield, they didn't need two backups, and they liked what they saw from Neild, especially on special teams.
Running backs Evan Royster and Keiland Williams have apparently been released, victims of overcrowding in a Redskins running back field now headed by Tim Hightower. Ryan Torain will remain on the roster, along with Roy Helu, as a top option if Hightower should falter. And it's a bit surprising to see them keep eight receivers, including Brandon Banks, Leonard Hankerson, Niles Paul and Donte' Stallworth.
No-brainers: They're only keeping two quarterbacks, John Beck and Rex Grossman, while cutting Kellen Clemens and Matt Gutierrez, but I don't think that's surprising. Those last two were only there to help take up preseason snaps when they needed to rest guys who might be their starters at any point this season. Rookie receiver Aldrick Robinson didn't do anything to help himself. And Artis Hicks has been squeezed out of the offensive line mix. They looked into trading Hicks Saturday but ultimately had to put him on waivers.
What's next: They could hit the market for a third quarterback, obviously, though that's not a major concern. I wonder if they'll still look for help at defensive end with Jarvis Jenkins out for the year. The Cowboys just cut Igor Olshansky, who's a name that will surely catch someone's attention.


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10194 Sumac Run, Littleton, Colorado
$925,0004 Beds3+ Baths
$925,0004 Beds3+ Baths
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