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High-throughput (HT) protein crystallography is severely impeded by the relatively low success rate of protein crystallization. Proteins whose structures are not solved in the HT pipeline owing to attrition in any phase of the project are referred to as the high-hanging fruit, in contrast to those proteins that yielded good-quality crystals and crystal structures, which are referred to as low-hanging fruit. It has previously been shown that proteins that do not crystallize in the wild-type form can have their surfaces engineered by site-directed mutagenesis in order to create patches of low conformational entropy that are conducive to forming intermolecular interactions. The application of this method to selected proteins from the Bacillus subtilis genome which failed to crystallize in the HT mode is now reported. In this paper, the crystal structure of the product of the YdeN gene is reported. Of three prepared double mutants, i.e. E124A/K127A, E167A/E169A and K88A/Q89A, the latter gave high-quality crystals and the crystal structure was solved by SAD at 1.8 Å resolution. The protein is a canonical α/β hydrolase, with an active site that is accessible to solvent.
Structural genomics, which synergistically involves both NMR and X-ray crystallography, is a global effort aimed at the determination of three-dimensional protein structures on a genomic scale in a high-throughput mode (Berman et al., 2000). Of particular interest in this early phase of the program is the determination of structures of proteins believed to represent new or barely known families. Every new structure deposited in the PDB increases the accuracy of homology modeling. This in turn allows better understanding of the structure–function relationships in those proteins for which structures cannot be directly determined experimentally (Stevens, 2000). However, the effectiveness of high-throughput (HT) crystallographic analysis is impeded by several bottlenecks, including the relatively low success rate of crystallization. It is estimated that of all proteins expressed in a soluble state, only between 10 and 30% form X-ray quality crystals (Claverie et al., 2002; Sulzenbacher et al., 2002). The most recent statistics available from the Midwest Structural Genomics Center (http://www.mcsg.anl.gov) show that of all successfully purified soluble proteins, less than 40% produced crystals, of which less than 60% were X-ray grade crystals that yielded useful diffraction. Thus, the first sweep of the structural genomics program is likely to harvest only the 'low-hanging fruit', i.e. readily expressed, stable and easily crystallizable proteins. Unfortunately, this may leave many biologically important proteins 'hanging high and dry'.
To circumvent one of the bottlenecks, i.e. the crystallization problem, we proposed a novel approach to crystallization of proteins based on rational surface mutagenesis to create patches with low overall conformational entropy in order to facilitate the formation of crystal contacts (Derewenda, 2004). This approach proved successful in a model system of human RhoGDI and allowed the crystallization and crystal structure determination of new proteins (Longenecker, Garrard et al., 2001; Longenecker, Lewis et al., 2001; Mateja et al., 2002). We and others have also shown that the method is useful to generate crystal forms that diffract to much higher resolution than the wild-type protein, which may prove to be of importance in drug design (Mateja et al., 2002; Munshi et al., 2003).
We are now applying this strategy to those selected targets of the Bacillus subtilis structural genomics effort which failed to crystallize in the HT pipeline. This paper describes the crystallization by surface modification and structure determination of the product of the YdeN gene from B. subtilis (Midwest Centre for Structural Genomics code APC 1086). The protein was originally thought to have a unique sequence and potentially a novel fold. Subsequent tertiary-structure prediction revealed that it shows similarities to α/β hydrolases and this information was used to design three double mutants, including the K88A/Q89A mutant, in which residues with high conformational entropy located on putative surface loops were targeted. The K88A/Q89A double mutant produced crystals of high quality that diffracted to 1.8 Å. The structure revealed that the protein is a member of the ubiquitous α/β hydrolase family and structural considerations suggest that it is a hydrolase active on a soluble ester, possibly a thioester, but is not an interfacially activated lipase. The molecular model, including H atoms and anisotropic displacement parameters, was refined to a conventional R factor of 12.4%.
The pMCSG7 expression vector containing the YdeN gene with a cleavable N-terminal hexa-His tag was obtained from the Midwest Center for Structural Genomics. Initial sequence-homology searches carried out at the beginning of the project indicated that the protein has no homologues of known structures and no identifiable domains. However, further analysis, including secondary- and tertiary-structure prediction using the Polish Bioinformatics Site Meta Server (Ginalski et al., 2003; Ginalski & Rychlewski, 2003; von Grotthuss et al., 2003), yielded results that strongly suggested that the protein belongs to the α/β hydrolase family. Despite the lack of identifiable sequence similarities, the fold-recognition algorithm generated an amino-acid alignment with bromoperoxidase A1, chloroperoxidase F, chloroperoxidase T (Hofmann et al., 1998) and 2-hydroxyl-6-oxo-6-phenylhexa-2,4-dienoic acid (HPDA) hydrolase (BphD enzyme) from Rhodococcus sp. (Nandhagopal et al., 2001). This alignment revealed the presence of three conserved canonical residues forming a putative catalytic triad, i.e. Ser, Asp and His, lending credibility to the result. As this work was in progress, similar annotation appeared in the NCBI Blast search.
Using a canonical model of an α/β hydrolase, three double mutants were designed so that the mutation sites were expected to be on solvent-exposed loops. The mutants were E124A/K127A, E167A/E169A and K88A/Q89A. The Quik-Change mutagenesis kit (Stratagene) was used to introduce the designed mutations into the expression plasmid.
The wild-type and mutated proteins were expressed in the Escherichia coli BL21(DE3) strain. Cells were grown in LB medium containing 100 μg ml−1 ampicillin at 310 K until the OD600 reached 1.2. Cultures were induced with 1 mM IPTG. Expression was performed at 293 K for 17 h. Cells were harvested by centrifugation at 6000 rev min−1 for 30 min, resuspended in buffer A (50 mM Tris pH 8.5, 300 mM NaCl and 10 mM imidazole) and subsequently lysed by sonication. The soluble fraction was obtained by centrifugation at 14 000 rev min−1 for 40 min. The protein was purified using Ni-affinity chromatography. The supernatant was incubated with 10 ml Ni–NTA agarose for 2 h to allow His6-tagged protein to bind to the resin. After collecting the flowthrough, the column was washed with 1.5 l buffer A. The protein was eluted in an imidazole gradient (75–150 mM) and dialyzed against 50 mM Tris pH 8.0, 1.0 mM EDTA, 0.5 mM DTT buffer for protease cleavage. The His6 tag was removed during incubation with rTEV protease for 48 h at 283 K. The cut protein was loaded onto an Ni–NTA agarose column to separate it from uncut protein, rTEV and His6 tag. The protein was dialyzed against 20 mM Tris pH 8.0 and concentrated to 15 mg ml−1 (Bradford assay, BioRad). The SeMet-labeled protein was purified in the same way as the native protein and finally dialyzed against 20 mM Tris pH 8.0, 200 mM KH2PO4 buffer and concentrated to 15 mg ml−1.
The preliminary screens with the wild-type and mutated protein samples were carried out using a screen including 96 solutions, a subset of the 108 best crystallization conditions reported by the Joint Center for Structural Genomics (Page et al., 2003). The sitting-drop method was used throughout. No crystals were obtained for the wild-type protein. The optimized crystallization conditions of the K88A/Q89A mutant involved mixing 1 μl protein solution and 1 μl reservoir solution containing 30% PEG 1500 and 0.2 M KH2PO4 pH 5.6. Crystals appeared overnight. For the SeMet-containing crystals, the well solution was 20% PEG 8000 and 0.05 M KH2PO4 pH 4.6. Crystals were frozen using the harvesting solution containing 20% PEG 8000, 0.05 M KH2PO4 pH 4.6 and 10% ethylene glycol.
Data were collected from a single SeMet-substituted crystal at the SER-CAT (Southeast Regional Collaborative Access Team) beamline 22-ID at the Advanced Photon Source, Argonne National Laboratory at nominal wavelengths of 0.97920 Å (peak), 0.97546 Å (remote) and 0.97931 Å (edge). All data were processed and scaled using HKL2000 (Otwinowski & Minor, 1997). The unit cell was identified as P212121, with unit-cell parameters a = 36.2, b = 54.1, c = 93.2 Å. The Matthews coefficient is 2.1 Å3 Da−1, assuming one polypep-tide per asymmetric unit.
Analysis of the MAD data using XPREP (Bruker AXS, Madison, USA) revealed incompatibilities with the assumed wavelengths, suggesting that the real wavelengths differed from the nominal values. The SAD method was used instead, based on the absorption-peak data. The anomalous substructure was solved with SHELXD (Schneider & Sheldrick, 2002). The two Se sites were fed into the autoSHARP procedure incorporating SHARP (de La Fortelle & Bricogne, 1997), SOLOMON (Abrahams & Leslie, 1996) and the CCP4 suite (Collaborative Computational Project, Number 4, 1994). The model was built automatically in ARP/wARP (Perrakis et al., 1999), which was able to build 184 out of 190 residues. The program O (Jones et al., 1991) was used for subsequent manual model improvement. Refinement using all data to 1.8 Å resolution was performed with REFMAC5 (Murshudov et al., 1997). The refinement of the model utilizing individual isotropic displacement parameters (B factors) converged with an R factor of 14.0% (Rfree = 18.4%). In the final stages of the refinement, H atoms were added to the model and anisotropic displacement parameters were used. This improved the agreement factors to 12.5 and 18.0%, respectively. MOLPROBITY (Lovell et al., 2003) and PROCHECK (Laskowski et al., 1993) were used as validation tools during refinement process. Relevant crystallographic data are shown in Table 1.
The refined model of YdeN includes residues 2–187 out of a total of 190 in the single polypeptide chain. The quality of the model was verified with PROCHECK (Laskowski et al., 1993), which showed that 88.0% of non-glycine residues are in the favored regions and 11.4% are in the allowed regions of the Ramachandran plot. Notably, Ser71 lies outside this range ( = 55, ψ = −117°). It is located within a tight turn and its strained conformation (ε region in the Ramachandran plot) is a characteristic feature of all α/β hydrolases (Derewenda & Derewenda, 1991; Ollis et al., 1992). One other residue, His18, also shows the ε secondary structure ( = 58, ψ = −134°) and the electron density for this residue is equally well resolved. The strained conformation of this buried residue is stabilized by both van der Waals and hydrogen bonds, including a close packing against the adjacent Trp19. The side chain of Arg87 is not visible in the σA-weighted 2mFobs – DFcalc electron-density map contoured at 1σ and it was consequently excluded from refinement. Six side chains (Asn41, Ser55, Gln58, Glu63, Glu124 and Asp147) have at least two conformers and the occupancies were adjusted to correspond to the electron density.
The refined YdeN model reveals the canonical tertiary fold of α/β hydrolases, albeit lacking the typically observed first two antiparallel strands (Fig. 1). Consequently, the molecule consists of six central parallel β-strands with the topology 213456 and eight α-helices. The strand–turn–helix motif following β3 has an architecture which is consistent with the nucleophilic elbow motif that is typical of all α/β hydrolases and is also observed in an unrelated enzyme, malonyl-CoA acyl transferase (Serre et al., 1995). The typical amino-acid sequence around the nucleophilic Ser in these proteins is Gly-X1-Ser-X2-Gly. The glycines are found in this motif because of the acute angle formed by the strand and the helix, which brings the side chains in these two positions into collision (Ollis et al., 1992). However, the first glycine in the elbow of YdeN is replaced with an Ala. This is not unusual; such departures from a canonical sequence have been observed before and are made possible by a slight opening of the elbow (Lawson et al., 1994; Uppenberg et al., 1994). The fingerprint sequence of the nucleophilic elbow of YdeN is similar to that of lipases from filamentous fungi, where X1 and X2 are also His and Leu, respectively (Derewenda et al., 1994).
Members of the ubiquitous α/β hydrolase superfamily share a common architecture consisting of a central predominantly parallel β-sheet motif connected by loops and helices. The β-sheets create a half-barrel in which the first and the last strand are twisted with respect to each other by approximately 90°. Insertions containing long Ω loops or even large subdomains frequently occur after strands β3, β4, β6, β7 or β8. These insertions typically participate in the formation of a substrate-binding site and define the enzyme's specificity (Heikinheimo et al., 1999). In many cases, such as those of the pancreatic lipase (van Tilbeurgh et al., 1993), bromoperoxidase A2 (Hofmann et al., 1998) or the 2-hydroxyl-6-oxo-6-phenylhexa-2,4-dienoic acid (HPDA) hydrolase (Nandhagopal et al., 2001), the insertion constitutes a 'lid' which undergoes structural rearrangement during the catalytic process. Smaller enzymes in this family, such as carboxylesterase (Kim et al., 1997) or cutinase (Martinez et al., 1991; Nicolas et al., 1996), have solvent-accessible active sites and are active against water-soluble small esters or thioesters.
The YdeN protein is a relatively small α/β hydrolase, smaller than cutinase (Martinez et al., 1991). The extra β-strand at the C-terminus (strand β6) arises from a difference in the tertiary fold in that region. Aside from a distinct similarity to cutinase, the tertiary fold of YdeN is also similar to the haloperoxidase, dienelactone hydrolase, esterase or serine carboxypeptidase subfamilies as defined by Heikinheimo et al. (1999). In common with the other small hydrolases, YdeN has no lid covering its active site, which is consequently solvent-accessible.
An automated comparison using DALI also identified a number of α/β hydrolases with a high level of structural similarity, including bromoperoxidase A2 (Hofmann et al., 1998; r.m.s. difference 2.3 Å over 170 amino acids), thioesterase (Lawson et al., 1994; r.m.s. 2.6 Å over 170 amino acids), dienoate hydrolase (Nandhagopal et al., 2001; r.m.s. 2.5 Å over 167 amino acids) and carboxylesterase (Kim et al., 1997; r.m.s. 2.4 Å over 161 amino acids). A structural comparison of the active centers shows that all the triads are very similar. The r.m.s. differences for all side-chain atoms of the triad residues are 0.56, 0.58, 0.33 and 0.25 Å, respectively.
The three catalytic triad residues always occur in the same order along the poly-peptide chain in all α/β hydrolases, i.e. first the nucleophile (Ser, Cys or Asp), then the carboxylic acid (Asp or Glu) and finally histidine. The three residues form an approximate mirror-image of the functionally analogous triad of serine proteases (Ollis et al., 1992; Derewenda & Wei, 1995). In YdeN this sequence is preserved and the three residues involved in the putative catalytic site are Ser71, Asp137 and His164. However, there is no hydrogen bond between the Ser hydroxyl oxygen and the imidazole of histidine because the hydroxyl group is shifted away from His164 Nε2. Instead, the hydroxyl forms a hydrogen bond with an adjacent water molecule (Fig. 2). A comparison of diverse enzymes containing classical triads shows that the geometry of the Ser-His interaction disfavors a strong hydrogen bond in the ground state (Z. S. Derewenda, unpublished work). In several cases, the hydroxyl of the serine is clearly turned away from the His (Ho et al., 1997; Devedjiev et al., 2000). In YdeN, the unpaired hydrogen-bonding potential of His164 Nε2 is satisfied through an interaction with an additional ordered water molecule in the active site. On the other hand, His164 Nε1 donates a typical hydrogen bond to Asp137 Oδ2 (2.74 Å). Asp137 Oδ1 is in turn held in its position by a hydrogen bond with the main-chain amides of Ile139 (2.81 Å) and Val140 (2.97 Å).
The function of the nucleophilic triad in triad-containing enzymes is complemented by the oxyanion hole, which stabilizes the incipient oxyanion during the course of the reaction via hydrogen bonds donated by amide groups (Matthews et al., 1975; Nicolas et al., 1996). These hydrogen bonds create a tetrahedrally distorted carbonyl C atom, which becomes susceptible to nucleophilic attack by the serine. The oxyanion hole can be formed by two amide groups, e.g. serine proteases and carboxylesterase (Kim et al., 1997), or three amide groups, as in acetylcholinesterase (Sussman et al., 1991) and the Streptomyces scabies esterase (Wei et al., 1995). In the last two cases, the oxyanion hole may be created by three main-chain amides or by two main-chain amides and one side-chain amide.
A close analysis of the stereochemistry of the putative active site of YdeN shows that main-chain amides of Tyr11 and Leu72 might serve to form an oxyanion hole. These amino acids correspond to Leu23 and Gln115 in carboxylesterase (Kim et al., 1997). There are no glutamine or asparagine side chains in the proximity of the active site that could contribute to the oxyanion hole.
YdeN belongs to a small family of homologous microbial proteins, including a conserved hypothetical protein from Mycoplasma penetrans (43% identity), a predicted esterase from Actinobacillus pleuropneumoniae serovar (40% identity) and a hypothetical protein from Photorhabdus luminescens (36% identity). The sequence alignment (Fig. 3) shows that all the residues that line the specificity pocket are closely conserved in this family, with only minor deviations. This suggests that all proteins may hydrolyze the same or closely related substrates.
The function of YdeN is not known, but the overall similarity to other α/β hydrolases strongly suggests that the protein is an enzyme with hydrolytic properties. Until very recently it was thought that all α/β hydrolases are enzymes, but the discovery of a non-catalytic protein from Mycobacterium tuberculosis with an analogous tertiary fold has changed this view (Wilson et al., 2004). Nonetheless, in the non-catalytic protein the nucleophile is absent and it is probably safe to assume that the presence of a complete triad is a strong indicator of hydrolytic function. Many α/β hydrolases are lipases, but the absence in YdeN of a lid that would allow interfacial activation suggests that it is active against water-soluble esters, although a thioesterase function cannot be ruled out.
There are a number of hydrophobic amino acids in close proximity to the triad, including Val140, Ile139, Leu109, Leu106, Leu103, Phe99 and His70. Together, they form a small but well defined specificity pocket (Fig. 2). Comparison with carboxylesterase from Pseudomonas fluorescens revealed that Ile139, Leu109, Leu106, His70 in YdeN correspond to residues that build the specificity pocket found in the carboxylesterase, i.e. Val170, Ile70, Met73 and Phe113, respectively. Moreover, all of these residues are conserved among sequence homologues of YdeN. We conclude that it is very likely that YdeN is a carboxylesterase active on a water-soluble ester in which the substrate, possibly the acyl group, has a hydrophobic nature.
As we predicted on the basis of modeling, the two mutated residues Lys88 and Gln89 are located on a sharp loop connecting helix C to strand 4. The loop is in close proximity to a crystal contact (Fig. 4), but the two alanines are not involved in any direct intermolecular interactions between molecules in the crystal lattice. The wild-type Lys88 would have pointed towards the contact, but it is unlikely that it would seriously interfere with the crystal packing. However, it is noteworthy that along with the preceding Arg87 a lysine in position 88 would contribute to a highly charged surface patch. The loop faces the N-terminus of the adjacent molecule, potentially leading to electrostatic repulsion assuming the wild-type sequence. It is therefore possible that unlike other successful examples of crystallization by surface engineering, crystals of YdeN are not formed becuase of a reduction of excess conformational surface entropy but because of altered electrostatic interactions. If indeed more than one mechanism is at play leading to high-quality crystals, this further increases the potential of surface mutagenesis for crystallization.
This work was funded by NIGMS (grant GM62615 to ZSD and, in part, grant P-50-GM62414 to AJ and WM). We would like to thank the staff of SER-CAT for help with data collection. Data were collected at Southeast Regional Collaborative Access Team (SER-CAT) beamline 22-ID at the Advanced Photon Source (APS), Argonne National Laboratory. Use of the APS was supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences under Contract No. W-31-109-Eng-38.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Biomechanics applies the principles and rigor of engineering to the mechanical properties of living systems. This book integrates the classic fields of mechanicsstatics, dynamics, and strength of materialsusing examples from biology and medicine. Fundamentals of Biomechanics is excellent for teaching either undergraduates in biomedical engineering programs or health care professionals studying biomechanics at the graduate level. Extensively revised from a successful first edition, the book features a wealth of clear illustrations, numerous worked examples, and many problem sets. The book provides the quantitative perspective missing from more descriptive texts, without requiring an advanced background in mathematics. It will be welcomed for use in courses such as biomechanics and orthopedics, rehabilitation and industrial engineering, and occupational or sports medicine.
In this book the authors explain the basic mechanical principles of the workings of the musculoskeletal system. The purpose is to teach biomechanics, using clinical settings, to a general audience who are seeking a better understanding of the workings of the human body. These most worthy objectives are fully met. This basic science book is written for orthopedic surgeons, physiatrists, rheumatologists, physical and occupational therapists, and athletic trainers. Other musculoskeletal researchers can benefit from reading this work. Both authors had experience teaching this material to medical professionals. The book is divided into three sections of 15 chapters. Basic concepts and application of mechanics, including system in equilibrium, are covered in the first section. In the second part the methods for analyzing deformation characteristics of materials are explored. The study of moving systems is covered in the third section. In all the sections the material is tied to human functions. Three appendixes cover plane geometry, vector algebra, and calculus. This second edition includes more biomechanical examples and exercise problems. Basic science education is an important requirement for most postgraduate doctoral degrees. For instance, orthopedic residents are expected to know the basic biomechanics of the musculoskeletal system. This book fills an important void in available reading material on the subject. I highly recommend it to orthopedists, orthopedic residents, and other residents involved in the care of patients with musculoskeletal problems.

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God Makes Us Strong
A sermon by the Rt. Revd. Bishop Paul Bayes, the Bishop of Hertford preached at St Luke's Church, Watford on November 6th, 2011.
The sermon was based on readings from Judges 7:2-22 and John 15:9-17.
The service was a service of Confirmation for 10 people from St Luke's, Watford, St Peter's, Watford and All Saints, South Oxhey.
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Sermon Topics: confirmation,Gideon


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Feds want to know if Tsarnaev will claim mental illness
Federal prosecutors want to know if accused Boston Marathon bomber Dzhokhar Tsarnaev will claim mental illness in his trial or, if convicted, during sentencing proceedings.
In court papers filed today, prosecutors asked "for notification in writing of any intention by the defendant to introduce expert evidence relating to a mental disease or defect or any other mental condition of the defendant which bears on either the issue of guilt or the issue of punishment."
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Application Details
Healthy Minds Canada/Pfizer Canada Workplace Depression Research Awards (Grants) 2016
Closes September 1, 2016
Eligible areas of research
- Any trial involving humans must be conducted in line with ICH guidelines and Canadian regulations (Health Canada).
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The 2 awards (grants) are up to $25,000 each. Award funds are intended to be operating grants and must contribute towards the direct costs of the research program or project for which the funds are granted. Please note that the budget must be inclusive of institutional overhead where applicable; this amount shall not exceed 20% of the grant application budget. Please note that applicants may not receive a salary, a stipend, or an honorarium from the Award (grant).
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- Applications will be received via email at [email protected]
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From the old Hollywood hair to the fringed hem and embellished bodice, Stone's look was undoubtedly one of our favourites from the night. See the dress from every angle below.


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Former Barcelona and Manchester United goalkeeper Victor Valdes has completed a switch to Premier League newcomers Middlesbrough, as revealed during an interview by head coach Aitor Karanka. The move is as unprecedented as it is unlikely given the rarity in which big name signings join newly promoted Premier League sides. After a seven year hiatus,
Last month the Barcelona goalkeeper Victor Valdes announced that he would not be renewing his contract with the club that ends in June 2014. He has been the first choice goalkeeper since 2003/4 and has won a staggering 19 titles with his beloved Barcelona. There has been a fairly mixed reaction to the news that


text: On Mon, 30 Oct 2000, Taro Fukunaga wrote: > > > Ethan Benson wrote: > > > > On Mon, Oct 30, 2000 at 10:27:18AM +0100, Chris Mueller wrote: > > > Hi Linux Friends, > > > > > > Does DebianPPC work on old MacPPC 6100? > > > 24 MB RAM, > > > 160 MB harddisk. > > > > > > Or does another Linux solution work with such a veteran? > > > > this is a NuBus based machine which the linux kernel does not > > support. however the freak cousin mklinux does. i have heard it is > > possible to install debian under the mklinux kernel but i am not sure > > how that would work. (i assume just boot mklinux with the debian root > > ramdisk and install as normal, if mklinux is compatible with the linux > > kernel it should work..) > > > > > Thanks for recommendations end experiences! > > > :-) > > > > i have friends who run mklinux and have found it not all that > > reliable, but perhaps that is due to the redhat userland mess normally > > associated with mklinux. perhaps debian under mklinux would work > > better... but i don't have any direct experience so i cannot say for sure. > > > I disagree, MkLinux is very reliable, but tends to be slow because of > its use of the microkernel. I am not sure if it's even usable (or > installable) on a machine with only 24 MB RAM. You can find more > information about mklinux at www.mklinux.org. > Again, I disagree, in part. The speed lag due to the microkernel is mostly negligible (of the order 5%), but 24 MB will be slow with any kernel. MkLinux itself is probably rather more stable than the monolithic kernels, certainly much more stable than the monokernel for Nubus under development. Also, I found pre-R1 userland much better organized than any of the other Redhat-based distros, in fact, I'm still using it with a monolithic kernel on my PB. With that machine you won't be able to run any fancy GUI (though plain X with blackbox might work nicely, if you manage to get it in 160 MB of disk space ;). MkLinux uses a special installer version of its vmlinux server, thus I don't think you could simply drop in a debian ramdisk. You might be able to install Debian from a minimal MkLinux install, though... In fact, I'm not positive if the installer currently works with 24 MB, it's quite close to the limit, but once installed, it would work with 24 MB. Feel free to ask on the newly re-created mklinux-users list for more info. Cheers, Derek


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And why would you be part of the UN? For your interests to be ignored, discarded? For your vote to be bought by the US? If at the beginning its intentions were "good" it definitely changed!
You can help the HubPages community highlight top quality content by ranking this answer up or down.
The "public" idea of the UN seems good, but the "hidden" idea is sinister.
A video I saw last week helps to explain the agenda behind the scenes:


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ART FOR JUSTICE: The First Tuesday Social Justice Film Series art film and silent auction will include original arts and crafts from local artists. The pieces range from $5 to $500 and include a range of media. Proceeds benefit the Unitarian Universalist Church of Oak Cliff's social justice ministry. 5:45 p.m. Tuesday. 3839 W. Kiest Blvd., Dallas. Free.firsttuesdayfilms.org.
MINI QUILT CLUB: Join other quilters to expand your modern quilting skills and learn new block styles. The Modern Patchwork Mini Quilt Club meets monthly and members make mini versions of each of the patterns in Modern Patchwork by Elizabeth Hartman. 6:45 p.m. Wednesday. CityCraft, 5460 W. Lovers Lane, Dallas. Free. 214-366-4220.citycraftonline.com.
Send items about crafts workshops, sales and other design-related events to email@example.com at least nine days before publication.


text: I'm James. I'm fairly new to Android. Last year I purchased a Palm Pre, and within 6 months it had to be replaced. Within 2 months that one had to be replaced. They had no more Pre's in warehouse, so I had the choice of the Palm Pixi, or the Samsung Moment. I chose the Moment, and I loved it. Except it did have a charging port issue. I recently got the EVO. And I love it.


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Wavecom Introduces Q64 Wireless CPU
Wavecom SA (Paris:AVM) (NASDAQ:WVCM) (ISIN:FR0000073066), announced the introduction of the Q64 Wireless CPU®, an enhanced version of the GR64 that is powered by Wavecom's Wireless Microprocessor® WMP100. According to Philippe Guillemette, Wavecom Marketing VP, "The Q64 will have a host of new features in the same popular, robust form factor as the GR64 thus facilitating integration into existing customer product designs. Customers appreciating the benefits of the GR64 will be able to continue using the form factor while taking advantage of the Open AT® Software Suite, without discarding existing design efforts."
BSQUARE to Distribute Solidcore's S3 Control Embedded Software
BSQUARE Corporation (Nasdaq:BSQR), a leading provider of embedded solutions, and Solidcore® Systems, Inc., a leading provider of real-time change control software, announced at the National Retail Federation (NRF) Annual Convention the signing of a strategic distribution agreement under which BSQUARE will distribute Solidcore's S3 Control Embedded(tm) software to original equipment manufacturers (OEMs). As a result, BSQUARE becomes an authorized distributor of Solidcore's S3 Control Embedded software, and the exclusive distributor of the software in North America.
Elo TouchSystems Announces 17A2 All-in-One 17-in LCD Touchcomputer
Continuing its history of innovative engineering, Elo TouchSystems has turned its recently announced 1729L 17″ integrated multifunction touchmonitor into a fully-functioning, fanless, all-in-one touchcomputer. The new 17A2 not only retains both the compact footprint of the 1729L and its comprehensive range of options, but also adds a host of new features to help customers gain an even firmer foothold in the point-of-service marketplace. Many diverse applications take advantage of the capabilities of the new 17A2 all-in-one touchcomputer including loyalty systems, kiosk information systems, home control, casino management, POS systems and Internet access points such as those for web surfing and hotel reservations.
2008 FTF Green Design Challenge Features $61,000 in Prizes
The registration deadline for the 2008 Freescale Technology Forum (FTF) Design Challenge Americas is January 31, 2008. This challenge encourages innovation in environmental design and offers compelling incentives to encourage designers to "go green." Freescale invites the engineering community to find creative ways to use less energy, limit the environmental impact of existing products, and create new products that help improve the environment. Participants will create innovative, green electronic designs using a select group of solutions from the company's broad product portfolio. Participants will retain the intellectual property rights to their designs.
Freescale Announces 200mm MEMS Production Line for Sensors
Freescale Semiconductor has established an advanced microelectromechanical systems (MEMS) 200-mm (8-inch) production line to address growing sensors market demand. The newly added line at Freescale's Oak Hill Fab in Austin, Texas, complements the company's existing 150-mm (6-inch) MEMS capacity in Sendai, Japan. High-volume MEMS manufacturing expertise remains critical as the MEMS sensor market continues to grow. Freescale has produced MEMS-based sensors for more than 25 years and is a leading high-volume sensor provider with an extensive portfolio of acceleration, pressure and proximity sensors. The company offers advanced embedded control capabilities with an extensive product portfolio that enables comprehensive sensing, control, connectivity and analog solutions.
Embedded IDE Link MU Integrates Simulink with Green Hills MULTI
The MathWorks and Green Hills Software, Inc. announced Embedded IDE Link(tm) MU, a new product from The MathWorks for Model-Based Design, that automatically deploys code generated from Simulink® models into the Green Hills® MULTI® integrated development environment (IDE), enabling seamless execution on a wide range of embedded microprocessors, including the Freescale(tm) Power Architecture(tm) (formerly called PowerPC®).
National Instruments Identifies Test and Measurement Trends
Test engineers in industries ranging from aerospace and defense to consumer electronics are facing the challenge of testing increasingly complicated designs with shrinking timelines and budgets. To address these issues, engineers and scientists are incorporating new test and measurement technologies that are capable of meeting complex design requirements without raising costs. National Instruments (Nasdaq: NATI), a worldwide leader in automated test solutions, has identified five trends it anticipates will significantly influence the test and measurement industry over the next three years.
Medical Imaging Equipment Drives Demand for FPGA, DSP, ASIC
The increasing requirement of high-end processors and field-programmable gate array (FPGA) in imaging equipment for high performance computing capabilities drives global demand for FPGAs, digital signal processors (DSPs) and application-specific integrated circuits (ASICs). New analysis from Frost & Sullivan, World Markets for FPGA, DSP and ASIC in Medical Imaging Equipment, finds that the market earned revenues of $602.87 million in 2006 and estimates this to reach $917.15 million in 2011.
Aeroflex 6113 Basestation Protocol Test System Gains Wideband AMR Support
Aeroflex has announced the addition of Wideband AMR (Wideband Adaptive Multi-Rate) support to its highly successful 6113 AIME basestation protocol test system. The new test capability relates to the 3GPP TS45.003 test specification. The new Wideband AMR codecs (TCH/WFS12.65, TCH/WFS8.85, TCH/WFS6.60) build on existing narrow band AMR technology and their introduction will significantly improve the quality of voice services due to the wider speech bandwidth used.
Bookham Creates ZoroLight LED for Biomedical Illumination
Bookham, Inc. (Nasdaq: BKHM), a leading provider of optical components for industrial applications, is to enter the biomedical market with its ZoroLight(tm) LED multiplexing technology. The compact LED combining module, which utilizes proprietary Bookham filter technology, is designed for bio-analytical applications and medical instrumentation. The ZoroLight LED module will be on show at the Bookham booth (#8636) at the BiOS exhibition, taking place 19-20 January at the San Jose Convention Center.
Lattice Debuts LatticeECP2/M FPGA Reference Design with TI ADS6000 Support
Lattice Semiconductor Corporation (NASDAQ: LSCC) announced its LatticeECP2(tm) and LatticeECP2M(tm) ("LatticeECP2/M") FPGA interface reference design supporting the Texas Instruments' ADS6000 family of analog-to-digital converters (ADCs). LatticeECP2/M FPGAs provide a high-speed glueless interface capable of acquiring 14-bit ADC data at rates up to 120 MSPS from the two to four serial channels found in ADS6000 ADC family devices.
Xilinx Ramps Up Virtex-5 SXT Devices for Production
Xilinx, Inc. (Nasdaq: XLNX), the world's leading provider of programmable solutions, announced that all of its digital signal processing (DSP)-optimized Virtex(TM)-5 SXT devices are available in production. These devices are part of the Xilinx(TM) XtremeDSP(TM) portfolio of solutions that includes development kits, design software, IP, and reference designs. The Virtex-5 SXT platform of devices is optimized for high performance and can deliver over 350 GMACs of fixed-point performance for many applications including wireless, test and measurement, and defense and up to 82.5 GFLOPS of floating-point DSP performance for applications such as medical imaging.
Korenix Introduces JetNet 4510 Industrial Managed Ethernet Switch
Korenix is pleased to announce the release of the new Industrial Managed Ethernet Switch — JetNet 4510. JetNet 4510 is a Managed Industrial Ethernet Switch, equipped with 7 ports 10/100TX and 3 ports 10/100 RJ-45 / 100FX SFP combo ports. The JetNet 4510 is designed as rugged surface in aluminum alloy material and with wide operating temperature. The software supports full L2 management features, ring redundancy, network control, security and alert features.


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Calgary, Canada (PressExposure) March 15, 2011 -- Why do I Say Potential Partner vs Prospect?
Calling people "prospects" implies your prospective partners are just another means for you to use to achieve your objectives. People will pick up on this even though you might not be aware of it.
Your words are the extension of your thoughts and they create an energy you carry with you and that people intuitively tune into. If you want to create relationships with potential partners, then what you say matters.
Keep in Mind when Calling
One vital point to keep in mind when calling your potential partner (lead) is: Do Not Pitch Your MLM Business Opportunity The point of your call is to find out more about them. It is all about them, NOT you. Do this properly and you will build trust and a solid relationship with your potential partners because you are not just another salesman trying to make a sale. You will also be able to weed out the tire kickers. You want to only spend time with people who are serious about building their business.
When your potential partners see how much you value them, they will feel appreciated and be more open to listen to any solution you may have to their problems. In turn, you will be able to increase your conversion ratio. Remember, they responded to your website and requested your help, not the other way around.
The Initial Contact
Use Your Potential Partner's Name Often. The sweetest sound to a human being's ear is the sound of their own name.
Start the conversation by saying:
Hello (prospect's first name), this is (your first name) and I am getting back to you. You had requested some information from my website about how to successfully build a home base business.
(Prospect first name), I am calling to see how things are going for you in your MLM company and see if there is anything I can do to help.
What you want to do is to get your prospective partner to talk about them. Find out where they are on their journey.
Start asking question, such as:
* So (Prospect), do you have any experience in the network marketing industry?
* How long have you been in the industry?
* What business are you promoting?
* How have your results been up to this point?
Again, use their name often and do not pitch your business.
Keep Initial Contact to 5 - 10 Minutes Maximum
You are a successful business owner and your time is extremely valuable. Even if you feel a connection with your lead after 10 minutes, say something like..."I would love to hear more about your experience, however, I am in the process of calling potential partners that has requested my help. Why not go back to (your lead capture domain), review the website and get a better feel of what we do here. You can call me with any additional questions.
Also, be sure to look out for some webinar invitations from me on a weekly basis. We provide the best marketing training on the plant from 7-figure master marketer. It was a pleasure meeting you and I'll see you on the inside."
The Follow-up Call
You must paint a picture for your potential partner. Find out what it is that is hurting them the most...
* Is it the fact that they have run out of people to talk to?
* Are they tired of using replicated websites that simply Do Not Work?
* Are they tired of struggling to generate leads?
* Do they hate their primary company?
Who knows, you might get a sign up in your primary right on the spot. Either way, whatever is the most painful detail of their business, you must be able to paint a picture and provide the solution their #1 problem. Really listen to what they're saying and focus... NO DISTRACTIONS. Over time you will learn to hear not just what they are saying, but what they are feeling.
When you paint that beautiful picture for your potential partner and you show that you are the solution to all of his/her problems, that you hold the key to the kingdom, here's what will happen...
* you just gained a new friendship
* a sign up in your marketing system
* a great shot at some serious affiliate commissions, and
* a soon-to-be distributor in your MLM business.
This is where the critical part of painting the picture comes in. Be sure to inspire them.
It's really that Simple!
How to Answer Some of Your Lead's Questions
Your potential partner will also be sizing you up and may have a few questions of their own. Here are examples on how to answer some of those questions.
Q: What is your primary business?
A: Go ahead and tell them. This is your opportunity to talk about your primary because they asked. Just remember, do not pitch your business.
Q: How many people do you have in your downline and how successful are they?
A: You cannot compare your potential success by how others are progressing. Everyone is different depending on their level of commitment to their business.
Q: How many leads are you generating and what is the conversion rate of those leads?
A: Again, you cannot compare how many leads you will produce by how many leads others are generating and converting. Ultimately, you will need to call your potential partners to produce a higher conversion rate. Stay in control of the conversation. Show your potential partner that you are a leader and can show them the way to success.
Become an expert in this process and your potential partners will learn how to know, like and trust you and you will get people to ask about your primary MLM company with credit card in hand ready to join whatever you tell them to.
Welcome to the Big Leagues. Now start calling your potential partners today!


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$21,000.00 1 month ago
2006 Mitsubishi Evo 9 GSR 91k miles - $21000 (Mankato)
PRICE DROP!As the title states, 91k on this Evo. Extremely clean in and out. Clean title with ZERO accidents on carfax. Front bumper/lip and hood were freshly repainted (too many rock chips for my liking). Brembos were also redone at same time. This thing is gorgeous #silverevosmatterVIN: JA3AH86C66U013039Price lowered to $21,000 from $23,000. Call or text me at if you're interested!Modifications:
1 month ago
Mankato, MN
$1.00 11 months ago
evo 9 mr - $1 (bristol)
for trade, i have a 06 evo 9 mr owned for 3 years, it has 119k miles on it!
11 months ago
Tri-Cities, TN
$27,500.00 1 year ago
Evo 9 mr se - $27500 (Locust valley)
I hate to do this but unfortunately its time to move on, so heres my 2006 evo 9 mr se, car has a brand new bouschur 2.0 with a brand new oem head gasket l19 head studs ,brand new plugs ,oil cooler,oil pump,water pump,and timing belt ,full brake flush all new diff fluids,car has nrg seats ,back is gutted i dont have rear seats .car has a sheepy mani v band with a 6466 with a 2pce down pipe,tear dro
1 year ago
New York, NY
Contact Us 1 year ago
Evo 9 mr
2006 Evo 9 mr. 125k miles Adult owned and always use correct fluids from Mitsubishi. Runs and shifts perfect. Nice 7 inch head unit with backup camera. 18 inch ssr 2 piece wheels, electronically controlled coil overs, cusco bracing and trunk brace. Turbo back exhaust, intake and intercooler piping. 350 hp and just smogged and will pass future smog tests with this setup. Best small car I've ever ow
1 year ago
Santa Barbara, CA
Contact Us
$22,500.00 1 year ago
2006 Mitsubishi Lancer Evolution ix MR SE (Evo 9, FP Red, Low Miles) - $22500 (Antelope)
Up for sale is my 2006 Lancer Evolution 9 MR SE package. Car is pretty flawless on the inside and out and in excellent condition. Apex Silver 2.0 Mivec Turbo w/ 6 Speed Transmission New exedy stage 2 Clutch and flywheel Rota Grid's with Bridgestone RunFlats FP Red BB Turbo on MHI Housing RC750 injectors 3" HKS TB Exhaust Defi Boost Gauge & Oil Pressure Gauge Flip Up Touch Screen Radio Stance Coilo
1 year ago
Sacramento, CA
$25,500.00 1 year ago
Mitsubishi Evo 9 SE - $25500 (Mankato)
2006 AS Evo IX SE 55xxx Miles: $25,500 or best offer Clean title: JA3AH86C86U064025 Have Carfax for it.Title is in hand and ready to go. Located in Mankato MN, 56001. Call or text: Details: Rare SE model, Stock 2.0 engine with 5speed transmission. Modifications: -AMS 3" Turboback exhaust-Walbro 255 Fuel Pump-GSC S1 Cams-Muse UICP -Mini battery/tray-MAP Exhaust Manifold Heat ShieldStock tune. Car r
1 year ago
Mankato, MN
$23,000.00 1 year ago
2006 Mitsubishi Evo 9 LOW MILES fs/ft - $23000 < image 1 of 3 > (google map)
Testing waters here. Putting up for sale or trade my evo 9. 61k miles. Tastefully modded. Please no low ballers.Engine:ARP head studsJSC stage 1 camsBeehive springs and retainersAMS full intercooler pipping and coreTial QRMegan exhaust manifoldPTE 1100cc injectorsE-85 tune by Devon Boostin performanceMegan racing exhaustMegan racing o2 housingDC down pipe and test pipeStock turboK&N typhoon intake
1 year ago
Show Low, AZ
$18.00 2 years ago
2006 Mitsubishi Evo 9 - $18 (Fall River)
The time has come to sell my Evo i would like to buy a house so here some info on the car .I got it when it had 36k has 60k now parts installed areEngine ETS Upper and Lower Intercooler pipesETS 3.5 inch Front Mount Intercooler ETS Intake FIC 1050cc Injectors Walbro 255 fuel pumpSynapse BOVTurbo XS downpipeInvida Test PipeMegan CatbackHellman Pro RX boost controllerInterior Sparco Harness Bar Susp
2 years ago
South Coast, MA
$28,900.00 2 years ago
RACE CAMS AFTERMARKET EXHAUST 37,000 MILES MANUAL ALL WHEEL DRIVEPASSES EMISSIONS car is very clean ... not too many in this condition or miles 30 DAY /1000 MILE POWERTRAIN WARRANTY BY DEALER we take trades we offer finance options 505 804 2727 for details calls or text
2 years ago
Clovis, NM


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"Ageless Creativity": The Artists of Shell Point About Shell Point Artists Does creativity decrease as age increases? Of course not! In fact, for many people, the retirement years finally provide the leisure to explore artistic interests, sometimes for the first time in their lives. Through new or renewed exploration of artistic pursuits, those who […]Find out more »
MERSEY BEATLES FOUR LADS FROM LIVERPOOL GET BACK TOUR This is not your ordinary Beatles' tribute band! The world's only All LIVERPOOL BORN Beatles' tribute band was formerly the resident band for a decade at the world famous CAVERN CLUB, where the Beatles got their start. They have played 20 countries, selling out shows worldwide. […]Find out more »


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2012 Stamp/Scrap On A Budget
View Single Post
Stampin' Fool
Join Date: Aug 2007
Posts: 1,393
Wow! Everyone is doing so great! I had a test yesterday, and I kept it below my budget. My ultimate goal was to spend nada, but my friends and I went to a store that we visit about twice a year, which is a real treat, because we have no crafting stores nearby. I did keep it below twentyfive though, which was my actual goal. I spent about eighteen on crafting stuff; the other ten was on pet, household stuff etc... Zero would have been better, but hey, it's a lot better than I've done in the past. I put lots of stuff back. I'm glad I'm not going crazy on the craft spending though, as it is helping me in other areas. I had decided to try to get stronger and lose weight (per doctor's advice also), so I got a wii at a pawn shop the other day. Going to try one of the dance games; I've heard good things about them being fun and a good workout. I think my health is a priority over my crafting. (Although, crafting is my therapy! lol.) I am also working on some cards for a charity, so I did pick up some reduced Christmas cards at Dollar Gen.. They were 75 percent off, so I got 32 enelopes for less than a dollar. I bought two boxes; going to keep the ones I like to give the ones who don't care anything about homemade cards, and using the rest of the envelopes for cardmaking. It's a good thing I got them mostly for crafting though; someone had rifled through the box, and swapped out most of the pretty cards in one box, and a few of the cards were damaged. Fortunately, the envelopes were fine,and I still got plenty of cards to mail out. I like repurposing and recycling, so they'll get used one way or another. I can even use the blank sides of the cards to stamp images to color and cut out.
Russ, I like using colored pencils also, along with some other coloring techniques. I haven't gotten into the copic thing either. I have a small room to craft in, and not enough room or $ to buy them. I'm not the most artistic either, so not sure I would do well with them anyway. I'm satisfied with the coloring mediums I have.
I'm enjoying this thread! Love having a place to go for support and sharing! Please overlook my long posts; I'm a chatty girl!
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Toyota of Puyallup
Review for Toyota of Puyallup
KRISTINE M. on 05/29/2015
"Fast, courteous service. Accurate estimate of cost."
All concerns with my vehicle were remedied in one easy visit for a fair price.
Vehicle: TOYOTA SIENNA
Category: Service
Service Date: 05/27/2015
Review Created: 05/29/2015 12:19 AM
Kerry S., President & General Manager at Toyota of Puyallup responded on 05/29/2015
Hi Kristine. Thanks so much for your valuable feedback. We're so pleased to hear you were satisfied with your experience at Toyota of Puyallup. We look forward to working with you again.Kerry Sawyer
President & General Manager
Toyota of Puyallup


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Scholarships 2011
Espinoza, Ada Carolina
George Mason University (Junior)
- $500 The Liberty Tax Service "Una Familia Sin Fronteras" Scholarship
Ada Carolina Espinoza is a junior at George Mason University majoring in Global Affairs with a concentration in Governance and Diplomacy and minoring in Economics. Ultimately her goal is to work for the U.S. Agency for International Development (USAID), the U.S. Department of State or the United Nations. When Ms. Espinoza came to the United States from Peru six years ago, she spoke little English. Less than a year later, she had learned the language, which she proudly admits is one of her greatest achievements. In the essay she submitted with her application as well as in her interview, Ms. Espinoza expressed the role that work ethic plays in achieving success. She describes Latinos as hard workers with dreams of success equal to those of the colonists who came to America. In her own words, "we fight for our dreams, goals and desires and we manage to accomplish them, because Latinos are people who never give up.
Gomez, Raymundo
Thomas Nelson Community College (Freshmen)
- $500 The Liberty Tax Service "Una Familia Sin Fronteras" Scholarship
Raymundo Gomez is a college freshman enrolled in the Thomas Nelson Community College 2X2 program, which allows students to transfer to any college within the Tidewater Consortium if they meet the required GPA. Mr. Gomez attained a 3.7 GPA in the first year Honors Program and has been accepted into Old Dominion University for the Summer 2011 semester. He plans to major in English and ultimately wants to become a teacher. As a believer that community service has been a road to his own redemption, Mr. Gomez has logged over 500 hours of volunteer work with the Special Olympics. He feels he has gained just as much from his commitment to the Special Olympics as they have benefitted from his involvement. Faced with challenges and adversity at home when he was in the ninth grade, Mr. Gomez says he lost hope and resigned himself to being a high school dropout. He credits a high school teacher for rescuing him from certain failure when she recognized his potential. In part his desire to teach in a public school is motivated by his wish to give back to the community that has supported and encouraged him. In his own words, "I want to become the kind of man who is improved by adversity, not one who is limited by it.
Jaimes, Milena
College of William and Mary (Junior)
- $1000 The VALHEN Latino Scholars Award
Transfer student from Richard Bland College GPA 4.0 at Richard Bland; Spring '11 is her first semester at W&M. Milena Jaimes transferred to the College of William and Mary after earning an Associate of Science degree from Richard Bland College with a 4.0 average. She is pursuing a major in Kinesiology & Health Sciences. Her long-term goal is to obtain a Doctorate in Physical Therapy. Her interest in this field comes from a wish to challenge herself intellectually as well as a desire to help others lead lives with little or no physical limitations.
Perez, Carolina
Virginia Commonwealth University (Freshmen)
- $1000 The VALHEN Mario Colon Rivadeneira Memorial Scholarship
Carolina Perez, a freshman at Virginia Commonwealth University majoring in Biology with a concentration in pre-medicine, entered college in the fall with thirtysix earned credits thanks to her scores on Advanced Placement exams. After graduating from VCU, she has aspirations of attending medical school and is considering becoming a primary care physician or internist "to reach out to as many patients from various backgrounds and walks of life as possible." Ms. Perez's extracurricular activities include volunteering at the emergency room of a hospital, helping to lead a Rosary Prayer group at the Catholic Campus Ministry; and acting as the newsletter chair for the Brandt Hall Council in her residence hall at VCU. Of Mexican ancestry, Ms. Perez is cognizant of the importance of family. She is thankful for the values her parents instilled in her and for the sacrifices they made to ensure that she and her brothers have better opportunities. As a first generation college student, she is motivated not only to strive for her goals, but also to be a positive role model for other young women.
Villalta, Ever
George Mason University (Sophomore)
- $500 The Liberty Tax Service "Una Familia Sin Fronteras" Scholarship
Ever Villalta is a sophomore at George Mason University with a major in Government and International Politics and a minor in intelligence analysis. His career goal is be involved in intelligence analysis that shapes strategies in foreign policy and provides intelligence necessary to maintain the United States' national security. He believes that a career with the government would allow him to serve a nation that has provided him and his family with so much. He would consider working for the United States Department of State or the Central Intelligence Agency. Mr. Villalta is actively involved with student government at George Mason University. He is a member of the Elections & Disputes Commission of George Mason University; a member of the George Mason College Democrats; a GMU Student Government Parking Appeals Board member and a GMU Mason Crew. Mr. Villalta expressed that he derives inspiration from his parents, who sacrificed themselves so their children could succeed. I've witnessed firsthand from my parents that hard work can go a long way and I am grateful for what I have. For that I seek to continuously capitalize on opportunities and set high goals.


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Lemartine has a distinctive richness combined with a classic elegance. It is a hearty, rich and elegant wine.
black and red berry fruits integrate to enhance a beautiful structure and
firm, yet never aggressive tannins. Its
generosity delivers minerality, intense fruit flavors and hints of
leather, spices and black licorice.
is the newest born of our wines, released for the first time in January
2012 with the 2010 vintage, which is certainly one of the most exciting
crops of the last 25 years.
like our first IGT wine "Madre", takes its name from one of three
old family homesteads built over a century ago on the estate. This
beautiful stone house, with breathtaking views over the surrounding
countryside, is embraced by graceful vineyards, olive groves and verdant
Making wine - red wine in particular - is our passion, and we trust it shows. Our unique terroir and privileged position is particularly well suited for growing a wonderful Sangiovese, and Brunello di Montalcino is, and always will be, our flagship wine and our first love. However, as with any true passion, we love to explore every aspect of our field and in 2001 we posed a new challenge introducing our first blended wine, Madre, which was a big success.
is well be known around the world for its Brunello, and this is a fact, but it
is without doubt a blessed region for growing red wines in general. Out of curiosity we planted
two varietals generally considered to be more "international" and
now, several years later, we are able to deliver wines with unique
complexity, flavor and minerality, thanks to this exceptional soil
Lemartine, the Brunello grape still plays the lead role, as it does in
all Poggio Antico wines, but here it is blended with Cabernet Sauvignon and
Petit Verdot (planted back in 1997 and 2003 respectively). These three
varietals, rigorously and exclusively estate grown at
is available in 750 ml. bottle format.


text:
I have had one of the Speedball Stamp Carving sets for a while and I have had it out this week to have a play with. I must admit I had been putting off using it as I imagined it being tricky and hard to use, but I was very surprised how easy it was to get quick and easy results with it.
I have been wanting some basic shapes to use in my journaling for a while now and I am very pleased with how these have turned out.
I simply drew my design onto a piece of paper and rubbed this face down onto the block transferring the image to the rubber. I then simply carved this using the tools in the kit.
I did find it easier to turn the rubber as opposed to the cutting tool to get nice even curves and to tidy them up I trimmed the out edges with a craft knife.
These will be great to use with acrylic paint as shown in my photo.
I used


text:
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Does SSHR have the capability to update an individual or group specific calendar?
Discussion in 'Oracle Forms and Reports' started by Watz61, Sep 28, 2011.


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- Simone Zuffanelli, Pau Aguila, Gerard Zamora, Ferran Paredes, Ferran Martín, Jordi Bonache
- 2014
- Simone Zuffanelli, Gerard Zamora, Pau Aguila, Ferran Paredes, Ferran Martín, Jordi Bonache
- IEEE Transactions on Microwave Theory and…
- 2015
The radiation properties of split-ring resonators (SRRs) at their second resonance frequency are studied for the first time in this work. In particular, the electric and magnetic dipole moments of the edge-coupled SRR are calculated analytically under the assumption of strong coupling between the internal and external rings. Based on these results, the… (More)
- Jordi Bonache, Gerard Zamora, Ferran Paredes, Simone Zuffanelli, Pau Aguila, Ferran Martín
- Scientific reports
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The definition of a precise illumination region is essential in many applications where the electromagnetic field should be confined in some specific volume. By using conventional structures, it is difficult to achieve an adequate confinement distance (or volume) with negligible levels of radiation leakage beyond it. Although metamaterial structures and… (More)
- Simone Zuffanelli, Pau Aguila, Gerard Zamora, Ferran Paredes, Ferran Martín, Jordi Bonache
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In this work, a passive ultra-high frequency radio-frequency identification UHF-RFID tag based on a 1.25 wavelengths thin dipole antenna is presented for the first time. The length of the antenna is properly chosen in order to maximize the tag read range, while maintaining a reasonable tag size and radiation pattern. The antenna is matched to the RFID chip… (More)
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text:
A little more than 24 hours ago, stock market futures were signaling one of the worst potential opening drops in history. Both the S&P 500 and Nasdaq indexes hit their circuit breakers of -5%, while the Dow Jones was down more than 800 points in after-hours trading.
And then, for reasons that will probably never be fully understood, stocks rallied.
And rallied.
And rallied some more.
Stocks rallied throughout the early morning hours, and actually opened up yesterday, which was an inconceivable of an open as it was unlikely that Donald Trump would actually win the election. None of the experts thought Trump had a chance, and absolutely no one believed that stocks would rally yesterday after his victory.
But yet the Dow Jones Industrial Average continued to rally throughout yesterday's trading session, closing up nearly 257 points to 18,589.69.
And futures this morning point to another positive open, with early Dow indicators up well over 100 points. If those gains hold into today's close, it would be a record closing price for the Dow — again, something almost no one thought would be possible, given a Trump victory.
Can it be that investors who believed just a few days ago that Trump would be toxic to the markets, now believe he's incredibly bullish for equities? Somehow, yes, that's exactly what appears to be happening.
Connor Campbell, financial analyst at Spreadex, said in a note to clients: "Just as Trump's victory is akin to a super-sized Brexit, the markets seem to have worked through the kind of fluctuations they saw following June's referendum in record time, compressing the sharp fall to all-time high-nearing rebound into around 24 hours."
"Investors who were previously so against the former reality TV star, appear to have had an abrupt change of heart in the short term," he said.
Here in the U.S., they say that everyone likes a winner.
It's probably safe to say investors are taking that saying to heart right now.
The SPDR Dow Jones Industrial Average ETF (NYSE:DIA) rose $1.30 (+0.70%) to $187.26 per share in premarket trading Thursday. Year-to-date, the only ETF that tracks the DJIA has gained 6.88%.


text:
Saturday, April 19, 2014
Family Matters
Today I found myself alone in my car, driving 40 minutes southwest towards Washington, DC. I was wearing dress pants, white shirt and tie and my best shoes. I should have been in heaven, but instead my trip took me far short of DC, and I instead ended up in the suburbs of Olney, Maryland.
This was a day of obligation and respect. I was on the way to family memorial gathering, but for someone wasn't family, but was.
ALL of my brothers are half brothers on my fathers side of the family. The funeral today was for their Uncle Marty.
Because one brother was in California, and the other in Ohio, I felt it was important that someone from my fathers family be there.
One of the things that I did after Dad died (and left us all a legal mess epic proportions) was to get in touch with the brothers Aunt. The Aunt, a lovely woman, had been cut out of the boys lives by my father. Once their mother was gone, that was that.
So I called her to tell her the beast was gone. So Aunt reclaimed her rightful position in my brothers lives.
Once we moved here, we thought about going to see her, but with Marty is rough shape, we never knew when was a good time.
And that brings us to today.
I know that I didn't inflict pain into her life and rob my brothers of a lifetime of love from their mother's only living sister. But I do feel that I should repair as much of the damage he caused.
Niecy and her brood showed up - and I accidently turned myself into the ogre of the day when I asked her three year old son to stop touching all of the cookies. OY!
And Aunt went around introducing me "I had a sister who passed, and she left behind her husband and two sons. Cookie is from my former brother in laws second marriage....and I can't tell you how shocked and glad that he is here!"
This impressed all of her friends when called me a "mensch".
I met the Aunt's best friend who asked where my wife was. "Well," he's at home..." She says "Old habits die hard. Is he a doctor? Because if he is, never complain about the hours he keeps."
I left after two hours and then drove home.
Along the way I thought what shame it was that my brothers spent most of their lives without their Aunt.
One of the great sadnesses of life is that funerals these days come to often. There used to be a time when they were rare events. But now they come to often and they take the people who have always been there, the people that we think, "oh, I'll call them next week," only they aren't there and its too late.
If there is someone in your life that you you keep putting off, stop and call them or better yet see them. Life is precious and it is fleeting. Embrace them now, instead of eulogizing them after they've passed.
Posted by The Cool Cookie at 4/19/2014 06:16:00 PM
5 comments:
you are such a nice person.ReplyDelete
Thank you for the truthful reminder.ReplyDelete
norma's watching what she says.ReplyDelete
Thank you, Cookie.ReplyDelete
Several months ago, I started making phone calls more often. I've also been sending notes via snail mail to my oldest relatives.
Last night I dreamed about one of my long-deceased relatives. I miss her so much and wish we'd had more time together.Replies
Just checked the mail. I received a card from my 96-old-year-old relative who is not supposed to write because of her painful arthritis but she wanted to thank me for all the notes I've been sending to her.Delete
I agree with you, Cookie. When they're gone, you can't do what you wish you'd done while they were here so put a little time aside whenever you can to contact them.
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text:
Status: Closed
Know more about this business than we do? Cool! Please submit any corrections or missing details you may have.Help us make it right
3 Brothers - Nick, Dino, and Gerry grew up in Italy and came to Kenilworth, NJ to open their own family-run Italian restaurant. For 28 years and counting the "three guys" own, manage, and cook at the restaurant.
Three Guys from Italy Restaurant and Pizzeria offers:
Restaurant Services - Lunch and Dinner
Private Parties
Take out
Be the first NJ.com user to add photos or videos of Three Guys From Italy
had their plain pizza. it was very good! i would buy from them again.
Bread came with butter packets that look like they belong in a diner. Dinner served with either pasta or salad, does not come with both. App over priced and nothing fancy. Chicken parm was not...
Three Guys From Italy is located at 19 N 20th St, Kenilworth, NJ. This business specializes in Pizza and has 5 review(s) with a star rating of 3.3. Three Guys From Italy is open Mon, 11am-3pm ; Tue, 11am-3pm ; Wed, 11am-3pm ; Thu, 11am-3pm ; Fri, 11am-3pm ; Sat, 4pm-10pm ; Sun, 2pm-9pm. and accepts American Express, Discover and Visa.
Three Guys From Italy can be found at N 20th St 19. The following is offered: Carry Out & Take Out. The entry is present with us since Sep 8, 2010 and was last updated on Nov 14, 2013. In Kenilworth there are 6 other Carry Out & Take Out. An overview can be found here.


text:
Recreation3 Dollar LunchAmenitiesBlues and Golds AwardsRecipientsClubsClubs DayCourses and TournamentsExam SpecialsFrozen DinnersGrantsRoom BookingsSauna BookingsRummage Sale
Otago Iranian Students
President: Faezeh Tashakori Sabzevar
Email: email@example.com
Otago Iranian Students Association is a non-profit and non-political organization founded in 2013 to give Iranian students a sense of home and help them to settle in Dunedin. The scope of activities covers the Iranian culture, arts, history, sports, literature, and music.
Producing cultural celebrations in honor of Persian holidays (Yalda and Nowroz)
Throwing parties and social gatherings to keep the Iranian students alive!!
Sharing information and experiences for the current and new students
Providing help and information to ease the way for the prospective students
Movie Nights


text:
Remote Technical Suppor tool kit.
Thank you for downloading SupportSmith IT Support from our download portal.
Current files are located on the following IPs: 188.8.131.5222.214.171.124
Outer mirror site one (www.supportsmith.com)
Outer mirror site two (www.supportsmith.com)
Note: We prefer to use WinZip, WinRar, WinAce, 7zip to extract zip, rar, 7z, ace files. For data iso files we recommend you use freeware Daemon Toos lite.
Note: All files listed on this site are tested by the ESET Smart Security. If you like to test this file by another security software we recommend you select some in our antivirus section.
desktop sharing, remote assistance, tight vnc, network console, realvnc ultra vnc, remotely anywhere, logmein, gotomypc, ssh, remote desktop, remote access, pc anywhere, remote control, teamviewer
Full downloadable version with free 30-day trial. This setup file can be freely distributed for trial purposes over the internet in an unchanged form.
For further distribution, purchasing or licensing information contact us: firstname.lastname@example.com
For technical questions email us to:


text:
U-Boat Left Hook 53mm brown dial
Sold for:
Start Free Trial
or Sign In to see what it's worth.
- Sold Date:
- Source: eBay UK
For Sale:Brand: U-Boat Series: Classico Movement: Quartz Serial: 0286 Case: 53mm (ex. Crown) Stainless steel (316L) Dial: Brown Glass: Mineral Strap: Original Army U-Boat strap used, condition ok Condition: - Case: Some small signs of wear, in good condition
- Glass: 1 small and light mark (Near center, visible on picture)The watch comes in itsoriginal black aluminum presentation box with card. Watch was purchased on 29/07/2006. Shipping: NL, DE, BE, UK: €15,- Other: On request
The item will be shipped within 2 working days after receiving payment.Item will be shipped traceable, insured and signed for delivery. Payment: Normal bank transfer Paypal
If you have any question feel free to contact me at any time.Detail pictures are available on request. See feedbacks for more happy U-Boat buyers. Also see my other auctions for more U-Boat watches! ! Please note: Because this is a private auction I cannot take any returns, give warranty or give any refunds.


text:
Carpenter Apprenticeship
Practical and technical training in years 1, 2, 3 and 4 of apprenticeship carpentry. In most cases, apprentices are required to attend one period (six-week session) of technical training in each year of their apprenticeship.
Upon successful completion of all four training years and the required number of practical work hours, the apprentice will obtain certification of carpentry qualification and will be permitted to write the inter-provincial examination for journeyperson status.
Admission Requirements
Limited admission
You must be indentured in this trade and hold a valid ITA-ID number. Currently there are waitlists for all apprenticeship programs. Adding your name to the waitlist is free and you will be contacted when your name comes up.
Next Steps
Add your name to the waitlist Visit the program website
Specific questions about this program?
Email: email@example.com
Phone: 250-828-5036


text:
It's Poem in Your Pocket Day, that magical day each year when you're encouraged to carry your favorite poem around with you and to share it with anyone who will listen–and even those who won't (if you shout it loudly enough, they'll be forced to pay attention.)
In honor of this auspicious occasion, we've devoted all of this week's Figment Daily Themes to writing prompts of the poetic persuasion, courtesy of the illustrious Emily Moore. An accomplished poet whose work has been published in The New Yorker, The Paris Review, Ploughshares, and the sixth edition of Poetry: A Pocket Anthology, Emily also moonlights as a high school English teacher in New York and a singer in the alt-country group, Ménage à Twang.
So try your hand at one or all of Emily's first four poetry prompts (for the fifth, you'll have to sign up for Daily Themes) and then share the resulting brilliance with a friend or Tweet it out with the hashtag #pocketpoem.
Figment Daily Theme – April 26, 2012
Here's a writing prompt that was inspired by Li-Young Lee's poem "Persimmons." Write a poem that weaves together three stories: a story involving words in a language other than English; a story about an object that someone in your family once brought home; and a story that begins with the phrase "In the sixth grade…" See what happens!
Figment Daily Theme – April 25, 2012
Write a poem about something that happened in the middle of the night.
Figment Daily Theme – April 24, 2012
Pablo Neruda wrote marvelous odes to common things, for instance in his poem "Ode to My Socks." Close your eyes, turn your head a bit, and when you open your eyes, write an ode to the first thing you see, no matter how humble. In the course of your ode, try to re-name your chosen object three times. For instance, in "Ode to My Socks," Neruda describes his socks as "cases / knitted with threads of twilight and goatskin," and says "my feet were two fish made of wool, / two long sharks." You can even attach a copy of your ode to the object you've written about; perhaps the next person who encounters that object will see it in new ways.
Figment Daily Theme – April 23, 2012
Take two minutes to jot down some memories that you have that involve motion–anything from biking to running quickly to swimming in the ocean to riding on a roller coaster. Once you've got a good list, choose one and try to write a poem in which the form of the poem–line breaks, rhyme, rhythm, line lengths, stanzas, anything! –expresses the motion you selected in some way.
If you haven't yet signed up for our Daily Themes emails, then whaddaya waiting for? Click here! And if you're eager to share your prompt response, tag your work with dailythemes and you just might get featured on the homepage.


text: As you can probably gather from my name, I'm lucky enough to live in Miami, just a few hours away from WDW! And incidentally, we are going this Saturday for the weekend! Hollywood Studios and Animal Kingdom So I have joined to take part in the exceitement and love of WDW with everyone else


text:
Let's discuss the key term female sexual dysfunctions. Sexual dysfunctions are disorders in desire, arousal, and orgasm. Sexual disorders described that are found specifically in women, include:
- female orgasmic disorder,
- and genital-pelvic/penetration disorder.
The first two disorders are characterized by persistent disturbances in the normal sexual response cycle.
Women with female sexual interest/arousal disorder demonstrate an absence or reduced interest in the sexual activity, or an ongoing inability to attain or maintain an adequate lubrication-swelling response during sexual excitement.
Women with the female orgasmic disorder are able to achieve a normal level of sexual excitement, but do not experience orgasm, experience delayed orgasm after the sexual excitement phase, or have orgasms infrequently or of reduced-intensity.
These disorders are diagnosed when the symptoms cause significant distress or interpersonal disturbance and are not solely due to the effects of a drug or another medical condition.
Genital-pelvic pain/penetration disorder involves they're being recurrent and persistent involuntary spasms in the outer third of the vagina that interfere with intercourse. These vaginal spasms occur when penetration (e.g., with a tampon, speculum, finger, or penis) is attempted; in some women, the anticipation of penetration is enough to induce muscle spasms.
The involuntary of vaginal contractions may be experienced as a tightening with mild discomfort or as a ripping, burning, or tearing pain that prevents penetration entirely. Genital-pelvic/pain penetration disorder is often associated with fearfulness or anxiety regarding intercourse. The condition may be exacerbated by a vaginal scarring due to child bearing, surgery, or abuse, irritation from the use of douches, spermicide, or condoms and infection.
Specifiers indicate whether the condition is lifelong or required, and whether the severity is mild, moderate or severe.
Sexual dysfunctions are usually caused by:
- performance anxiety,
- unrealistic expectations,
- misinformation,
- early conditioning,
- and/or ignorance of sexual physiology.
Sexual dysfunctions in both men and women are divided into subtypes on the basis of onset, context, and etiology. Sexual dysfunctions that have been present since the onset of sexual functioning are classified as lifelong, while those that appear after a period of normal functioning are classified as acquired type. Sexual dysfunctions that appear in a variety of contexts are classified as generalized type, whereas those that are limited to specific forms of stimulation, situations, or partners are classified as situational type.
Treatment approaches employ a variety of methods, including:
- relationship counseling,
- cognitive restructuring,
- Female libido supplements and creams
- and sensate focus.
Women with the female orgasmic disorder may receive instruction in and be encouraged to engage in masturbation. Vaginismus is often treated with relaxation exercises as a gradual dilation of the vagina. Women with sexual dysfunctions may also improve their sexual functioning and enhance sexual pleasure through the use of Kegel exercises, which involve repeated and voluntary tightening of the pelvic floor muscles.
Let's practice with a question for female sexual arousal disorder. In female sexual arousal disorder compared to orgasmic disorder the primary problem relates to all of the following, except:
- lubrication
- swelling
- climax
- vasocongestion
Answer 3 is correct as lubrication, swelling of external genitalia, and nasal congestion of the pelvic region are all associated with arousal disorder, whereas the orgasmic climax is a primary problem for the orgasmic disorder.


text:
Dick Shawn (born Richard Schulefand; December 1, 1923 - April 17, 1987) was an American actor and comedian. He was well-known for appearing as Lorenzo St. DuBois in the Mel Brooks film, The Producers and as the voice of Snow Miser in The Year Without Santa Claus.
His only Disney role was portraying Captain Bog in Captain EO.


text:
Come enjoy the garden and help with watering. We have 12 weeks during the summer when the garden needs watering, If you have any time when you could help between June 16 and September 1, please contact me.
The weeks of July 7, July 14 and July 21 are covered by moms who will be teaching gardening at summer camp.
Priscilla Lane, Garden Coordinator, email@example.com


text:
- Promotion Activity
- HMI (LEVI Series)
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- Wince Panel (Levi Series)
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GraphicRiver 105 Circular Textured Icons 136599
Stock Graphic \ Icon \ Web
Layers \ Circular \ Simple \ Minimalist \ Texture \ Included \ Circle \ Separate
Icon - 105 Circular Textured Icons 136599 by NudgeD @ GraphicRiver
Stock Graphic \ Icon \ Web
This web icon pack is a 3 color set of simple circular icons with subtle texture.
- Set comes in black, green and blue.
- It includes PSD file with layers, png file, and a jpg file.
- Separate layers in PSD for each icon and color.
- Also included are the icons without background, in black and in white.
- Each circular icon is 72×72.
Graphics Files Included: JPG Image, Photoshop PSD, Transparent PNG.
Holiday Icon set.
Keywords: black, blue, circle, design, elements, green, icons, minimalist, minimalist, portfolio, retro, simple, texture, transparent, vintage, white.


text:
Yakumo Tatsu
Type OVA
Airing Status Finished Airing
Duration 29 min. per episode
Air Date Oct 25, 1997
Season fall 1997
Producers Studio Pierrot, Bandai Visual, Media Blasters
Genres adventure historical horror shoujo

Fuzuchi Kuraki is a quiet young high school student blessed with immense psychic powers and an ancient sword. He is searching for other magical artifacts with the help of Nanachi Takeo, a college student with latent powers of his own. They delve deep into the dark magic of Izumo, only to discover the secrets buried within the birthplace of all Japanese mythology.

 (Source: ANN)

Recent EpisodesAll Episodes
No Episodes Found
Anime Relations
No Relations Found.


text:
The Elite Skeleton Rack from Raxxess is designed for special installations where
aesthetics are paramount. Four brushed and anodized aluminum posts surround
vinyl clad MDF sides, finished in either textured black or silver to simulate a
clear brushed aluminum. The top is again vinyl wrapped 3/4 inch MDF finished in
a rich and complimentary carbon graphite pattern. The bottom is black vinyl over
3/4 inch MDF. The ESKR comes knocked down and is easily assembled in just a few
minutes. Available in 24 rack spaces. Raxxess Logo Panel not included.
Overall dimensions are 22 inches wide, 23 inches deep and 43.625 inches tall.
What started as a simple string and pedal sales catalog has grown exponentially over the past thirty years. The early days were a time where catalogs didn't have much of a presence in the industry, but what began as a college dorm room operation grew rapidly. In 1986 we moved to a full product offering and 64-page catalog, which over the years has grown to 162 pages. Join the AMS family and get your free catalog now!
Copyright © 1996-2017 American Musical Supply, Inc. All rights reserved. American Musical Supply reserves the right to correct pricing and/or product specifications in the event of typographical errors found in our print catalog or on our website. If a product is listed at an incorrect price due to such errors or because of inaccurate information received from a supplier, American Musical Supply shall have the right to refuse or cancel any orders placed, whether or not the order has been confirmed and your credit card charged. If your credit card has already been charged for the purchase and your order is canceled, American Musical Supply will issue a credit to your credit card account in the amount of the charge.


text: Getting the dread "low space" notification. I don't have tons of apps (sorted by size, Touchdown is the biggest), I clear the caches regularly and delete old text messages, which usually makes the notification go away, but this time it didn't. I'm rooted, using Aloysius Snow, which supposedly has app2sd, but I haven't been able to find it. Would like to use cache2cache, but want to keep my ROM. I've read through other threads and don't find any terribly useful advice. Any help gratefully appreciated!


text: I have an odd issue with my contacts. When I go to the default contacts from the home screen I can see all of my contacts, including a lot of duplicate entries where one person will have 2 contact cards, 1 with a plain phone number, and 1 with their number labeled "mobile" (... through research found that the plain-numbered versions are probably simcard saved contacts, which can only contain 1 number per contact) all the apps that use the contacts (ie. hancent) refuse to recognize contacts saved in the sim format, so when i get a text from these people it just comes up with their number. All my contacts look fine in Gmail on the web and I even did a "merge contacts" command on duplicates to try and solve the issue. Looks fine on the web but my phone still has all these dupes, any ideas?


text:
We take Scuba Diving to a whole new level!
First of the twice annual trips to the Florida Springs area. Starting January 2013 Virgina Scuba started running trips twice during the winter, one week in January and one week in March to the High Springs area of Florida to fight off Cabin Fever and fill the itch to go diving.
As well we offer this to our student who have completed the confined water part of their Open Water course as a way to complete their open water dives. The weather is great, the water at the various springs is always in the mid-70s. A great week of diving fun.
Devils Run
Devil's Eye
Cavern Diving Florida Springs
Rescue Dive Training
Night Dive
Boat ride to snorkel with the Manatee
Manatee Encounter Florida Springs
Gearing Up
Diving Florida Springs with Virginia Scuba
Diving Florida Springs with Virginia Scuba
Rescue Training at Ginnie Springs
Rescue Training at Ginnie Springs
Diving Catfish Hotel
Manatee Encounter
Tom Diving Catfish Hotel
James Diving Florida Springs
After the Manatee Snorkel
Catfish Hotel
Looking Up
Tige Looking for Treasure
Pretty Fish
Reflective Diving
The Aftermath
Looking Out of the Cavern
Tige and Mike at Rainbow River
You Call Me TOM!!!!!
Tom Exiting Cavern
Mike at Rainbow River
Hanging Loose at Rainbow River
After the Diving
Virginia Scuba is a full service dive shop and snorkeling center serving Northern Virginia, Maryland and the greater Washington DC area.
We offer recreational scuba diving classes from the beginner level (open water) through Instructor.
We are also the area's premier technical dive shop offering all levels of tech training, including mixed gasesfor both open circuit and closed circuit rebreathers.
We train divers through SSI, TDI and IANTD.