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To understand regulation of transplanted hepatocyte proliferation in the normal liver, we used genetically marked rat or mouse cells. Hosts were subjected to liver injury by carbon tetrachloride (CCl4), to liver regeneration by a two-thirds partial hepatectomy, and to hepatocellular DNA synthesis by infusion of hepatocyte growth factor for comparative analysis. Transplanted hepatocytes were documented to integrate in periportal areas of the liver. In response to CCl4 treatments after cell transplantation, the transplanted hepatocyte mass increased incrementally, with the kinetics and magnitude of DNA synthesis being similar to those of host hepatocytes. In contrast, when cells were transplanted 24 h after CCl4 administration, transplanted hepatocytes appeared to be injured and most cells were rapidly cleared. When hepatocyte growth factor was infused into the portal circulation either subsequent to or before cell transplantation and engraftment, transplanted cell mass did not increase, although DNA synthesis rates increased in cultured primary hepatocytes as well as in intact mouse and rat livers. These data suggested that procedures causing selective ablation of host hepatocytes will be most effective in inducing transplanted cell proliferation in the normal liver. The number of transplanted hepatocytes was not increased in the liver by hepatocyte growth factor administration. Repopulation of the liver with genetically marked hepatocytes can provide effective reporters for studying liver growth control in the intact animal.
- carbon tetrachloride
- hepatocyte transplantation
- liver regeneration
the regenerative potential of the liver has long been recognized (24), although there has been considerable debate concerning the relative role of hepatocytes and putative progenitor (or stem) cells in this process under various circumstances (1, 4, 22,35-37). Recent studies (2, 3, 31) indicated that progenitor liver cells can be isolated, with the potential to differentiate either completely or partially along the hepatocyte lineage in suitable microenvironments. On the other hand, when host hepatocytes are chronically and extensively depleted, such as in the albumin-urokinase-type plasminogen activator transgenic (Alb-uPA) or fumarylacetoacetate hydrolase (FAH)-deficient mice, transplanted hepatocytes can repopulate the entire liver without involving progenitor cell activation (27, 30). Indeed, serial cell transplantation studies with FAH-deficient mice proved that hepatocytes isolated from adult mouse donors can replicate indefinitely during repopulation of the diseased liver (27). These findings suggest that, in appropriate pathobiological settings, such as fulminant hepatic failure due to extensive loss of parenchymal liver cells, transplanted hepatocytes should proliferate significantly if at a selective advantage to do so and, consequently, help improve survival (13). However, whether similar concepts concerning liver repopulation could be applied to proliferation of transplanted hepatocytes in the normal liver has not been well established.
To establish mechanisms concerning proliferation of transplanted hepatocytes in the normal liver, we performed a series of studies in rodent models. Our general hypothesis was that in the presence of suitable mitogenic signals, transplanted hepatocytes will proliferate in the normal liver. We further considered that, on integration into the hepatic parenchyma, transplanted cells would participate physiologically in ongoing processes in the liver similar to those affecting host hepatocytes. We utilized recently developed genetic reporter systems for our studies, including a transgenic hepatitis B virus (HBV) cell transplantation system in which transgenic cells expressing HBV surface antigen (HBsAg) are transplanted into congenic mice (10, 11, 14) and the dipeptidyl peptidase IV-deficient (DPPIV−) F-344 rat system in which F-344 rat hepatocytes are transplanted into syngeneic mutant animals (12, 13, 28, 29). The former system allows estimates of the transplanted hepatocyte mass by serum HBsAg measurements (11), whereas the latter system offered convenient strategies for in situ localization and dual-label studies to analyze proliferation in transplanted cells (12, 28). Previous work by Rajvanshi et al. (28) established that transplanted hepatocytes are distributed in periportal areas (zone 1) of the rodent liver, allowing consideration of strategies to spare transplanted cells from ablative injury.
Our major goal here was to determine how transplanted hepatocytes responded to proliferative stimuli in the normal liver. The experimental perturbations were to ablate liver cells in a targeted fashion by carbon tetrachloride (CCl4), which depletes perivenous hepatocytes (zone 3 of the liver lobule) (23), to perform a partial hepatectomy, which induces highly synchronous DNA synthesis in the remnant liver (16, 24, 38), and to administer hepatocyte growth factor (HGF), a potent hepatic mitogen (24, 25, 32) that is released after both partial hepatectomy and toxic liver injury such as by CCl4 (20, 21).
Animals. Male F-344 rats serving as hepatocyte donors were obtained from the National Institutes of Health (Bethesda, MD) and weighed 250–300 g (12–16 wk old) when used. C57BL/6J mouse recipients weighing 15–20 g (6–8 wk old) were from Jackson Laboratories (Bar Harbor, ME). Syngeneic, DPPIV− F-344 recipient rats (160–180 g) and congenic G26 HBV transgenic donor mice (25–30 g) were provided by the Special Animals Core of the Marion Bessin Liver Research Center (Bronx, NY). The studies used animals in multiple groups composed of at least three to four animals each under protocols approved by the institutional Animal Care and Use Committee in accordance with theGuide for the Care and Use of Laboratory Animals [DHEW Publication No. (NIH) 85-23, revised 1985, Office of Science and Health Reports, DRR/NIH, Bethesda, MD 20505].
Cell transplantation by intrasplenic injection in rodents was performed as previously described (14, 28). The animals were anesthetized with inhaled ether (Fisher Scientific, Fairlawn, NJ). Rats received 2 × 107 cells and mice received 2 × 106 cells suspended in RPMI 1640 medium. A two-thirds partial hepatectomy in rats was performed according to Higgins and Anderson (16). A partial hepatectomy in mice, which required leaving a collar of tissue adjacent to the gallbladder bed and bile duct, was performed as previously described (38). After the abdomen was closed, the animals were monitored closely until recovery. To induce hepatic injury, 1.45 ml/kg of CCl4 was administered intramuscularly once after dilution to either 1:1 or 1:2.5 (vol/vol) in mineral oil for rats and mice, respectively (Sigma, St. Louis, MO). To analyze changes in individual animals, serial wedge liver biopsies were performed via a midline laparotomy in recipients of CCl4 as previously described (29).
For growth factor-induced hepatic DNA synthesis, we used recombinant human (h) HGF (lot 18181-25, Genentech, South San Francisco, CA). The hHGF was >90% two chain as shown by densitometry of reduced, purified protein by SDS-PAGE (25). To administer HGF, Alzet osmotic pumps (Alza, Palo Alto, CA) were primed overnight, and injection ports were inserted into the spleen of animals with P-60 tubing (VWR Scientific, West Chester, PA). The tubing was secured by an encircling ligature around the splenic lower pole while the proximal end was tunneled subcutaneously to connect with the pump implanted on the back of the animals. For mice, Alzet 2001 pumps were used, with infusion rates of 1 μl/h, and for rats, Alzet 2ML1 pumps were used, with infusion rates of 10 μl/h. The hHGF was diluted in 0.5 M NaCl and mixed with an equal amount of dextran sulfate as previously described (32). The dose of hHGF administered was 2.4 mg ⋅ kg−1 ⋅ day−1for 7 days.
Hepatocyte isolation and culture.Hepatocytes were isolated from donor animals with a two-step perfusion with 0.025% (wt/vol) collagenase D (Boehringer Mannheim, Indianapolis, IN) as previously described (14, 28). The cell viability was tested by trypan blue exclusion as well as by attachment to tissue culture plastic in RPMI 1640 medium containing 100 U/ml of penicillin, 100 μg/ml of streptomycin, and 10% heat-inactivated fetal bovine serum (GIBCO, Grand Island, NY). To document mitogenic activity of hHGF, hepatocytes were cultured on rat tail collagen-coated dishes at 2.5 × 105cells/cm2 with and without 20 ng/ml of hHGF for 48 h. DNA synthesis was measured by incubating hepatocytes with [3H]thymidine (specific activity 50–70 mCi/mmol; ICN, Irvine, CA) for 1 h. The cells were then lysed in 0.3 M sodium hydroxide, TCA-precipitable DNA was extracted, and [3H]thymidine incorporation was determined along with microfluorometric quantitation of DNA in aliquots as previously described (9).
DNA synthesis measurements in tissues.To determine the magnitude and kinetics of liver regeneration with the procedures used, groups of three mice and three rats each were given 0.5 μCi [3H]thymidine/g body wt intraperitoneally 1 h before the animals were killed at various times after the interventions. The tissues were subjected to autoradiography with NTB-2 emulsion (Eastman Kodak, Rochester, NY) for 4 wk as previously described (11). To determine labeling indexes, two independent observers counted 10,000–15,000 hepatocytes/section in a blinded manner.
In some animals, H3 histone mRNA expression, which correlates with DNA synthesis, was analyzed with previously described specific probes (38). A 280-base pair fragment was used from the coding sequences of human albumin cDNA (plasmid pHSA1), which binds mouse albumin mRNAs (38). To generate antisense and sense RNA probes labeled with digoxigenin-11-UTP, commercial kits were used according to the manufacturer's instructions (Boehringer Mannheim). In situ hybridization was on 5-μm-thick paraformaldehyde-fixed cryostat sections under stringent conditions (11, 14, 38). After hybridizations, probe binding was localized with an anti-digoxigenin-alkaline phosphatase conjugate according to the manufacturer's instructions (Boehringer Mannheim).
For localization of DNA synthesis in transplanted cells, animals were given a 2-h pulse of 50 mg/kg of bromodeoxyuridine (BrdU; Boehringer Mannheim). To detect BrdU incorporation, cryostat sections were subjected to immunostaining with a commercially available antibody system (Amersham, North Chicago, IL). The tissues were blocked with 2% rabbit serum, and antibody binding was detected by a supersensitive multilink antibody system with the peroxidase reporter (BioGenex Laboratories, San Ramon, CA), followed by color development with a Vectastain kit (Vector Laboratories, Burlingame, CA).
Localization of transplanted cells. To localize transgenic G26 HBV hepatocytes, in situ hybridization was performed on cryostat tissue sections with35S-dUTP-labeled HBsAg probes as previously described (11, 14). The sections were autoradiographed at 4°C with NTB-2 emulsion (Eastman Kodak) for several days before being developed.
To localize F-344 rat hepatocytes in DPPIV− animals, histochemical staining was performed as previously reported (12, 28,29). Integrations of transplanted cells in the liver parenchyma were demonstrated by colocalization of DPPIV activity and bile canalicular ATPase activity. To colocalize DNA synthesis in transplanted cells, tissues were first subjected to DPPIV staining, and this was followed by immunostaining for BrdU incorporation. Tissues were counterstained with methyl green or hematoxylin as appropriate.
Morphometric analysis. To determine changes in cell number in the tissues, wedge biopsies taken at 4 wk after cell transplantation from three individual rats before initiation of CCl4 treatment and final tissue samples obtained at 36 wk after cell transplantation were analyzed with histochemical staining for DPPIV activity. The number of cells per portal area was determined in individual tissue sections for quantitative morphometry. A minimum of 50 portal areas were analyzed in each tissue. For additional analysis, changes in cluster sizes were determined by analyzing a minimum of 250 consecutive areas with transplanted hepatocytes in each tissue. For this purpose, transplanted cells in consecutive areas were scored for the number of cells per cluster: 1–3, 4–6, 7–9, 10–12, 13–15, and >16 cells each. Similar analysis was conducted to determine whether the cell number changed in animals subjected to other mitogenic treatments.
Serological assays. Blood was sampled at 3- to 7-day intervals from the tails of animals, and the sera were stored at −20°C for analysis. Serum HBsAg was measured with a commercially available radioimmunoassay (AUSRIA II, Abbott Laboratories, North Chicago, IL). HBsAg standards were included in a known range of concentrations as previously described (11, 14). The baseline serum HBsAg levels were used to normalize subsequent serum HBsAg levels in individual animals for comparisons.
Statistics. Data are expressed as means ± SD. SigmaStat 2.0 software was used for data analysis (Jandel, San Rafael, CA). The significance of variances was analyzed as appropriate by Student's t-test, χ2 test, Mann-Whitney rank sum test, and ANOVA.
Characteristics of isolated hepatocytes. The viability of isolated rat and mouse hepatocytes ranged from 85 to 90% as assessed by trypan blue dye exclusion. The cells promptly attached to tissue culture plastic (>60% attachment after 1 h) and assumed normal parenchymal morphology in culture. In addition, both mouse and rat hepatocytes responded to hHGF, with DNA synthesis rates increasing by four- to sixfold in comparison with unstimulated control cells at 48 h (P < 0.001). These data verified the integrity of isolated cell preparations and indicated that our hHGF was bioactive.
Magnitude of liver regeneration induced. To document that various maneuvers induced liver regeneration, we studied C57Bl/6J mouse and DPPIV− F-344 rat hosts before proceeding with subsequent experiments.
The kinetics of DNA synthesis after a two-thirds partial hepatectomy in rats was similar to a previous experience, with an early peak at 24 h, followed by rapid attenuation of the regenerative response (Fig.1 A). In contrast, after CCl4administration, the initial peak was delayed and DNA synthesis was characterized by a relatively lower magnitude as well as a longer persistence compared with partial hepatectomy in both mice and rats. Also, after partial hepatectomy, DNA synthesis was apparent in hepatocytes essentially throughout the liver lobule, whereas CCl4 treatment resulted in DNA synthesis in the periportal areas because perivenous hepatocytes were destroyed by the toxin (Fig. 1 B). The distribution of hepatocytes containing either [3H]thymidine or BrdU was similar to the distribution of hepatocytes expressing histone H3 mRNA (Fig. 1 C) as also previously observed (38). In the dose used, CCl4 depleted hepatocytes from only ∼30 to 50% of the liver lobule (Fig. 1,D–F), which most likely accounted for the difference in the magnitude of DNA synthesis compared with that resulting from a two-thirds partial hepatectomy. The hepatic susceptibility to CCl4 is determined by the activity of specific cytochrome P-450 isoforms in individual cells, with perivenous hepatocytes being most sensitive (21).
After hHGF infusion in the animals, we analyzed DNA synthesis rates at 60 h after commencing the infusion, which has previously been shown to be effective in intact animals (32). Studies were performed in three mice per group and showed significantly increased BrdU incorporation compared with untreated control animals (Fig. 1,G andH). The overall hepatocyte labeling index approached 42 ± 6/1,000 cells, which was less than that observed with either a two-thirds partial hepatectomy or CCl4 treatment, although this compared favorably with DNA synthesis after a 35% partial hepatectomy in a previous study (38).
Effect of CCl4-induced liver regeneration on transplanted cell proliferation.
The strategy was based on the fact that intrasplenic transplantation deposits cells in periportal locations (28). The hypothesis was that the susceptibility of perivenous hepatocytes to CCl4 would not extend to transplanted cells located in periportal areas and that transplanted cells would participate in the ensuing proliferative response of host hepatocytes in nonablated areas.
The experimental strategy was to let transplanted cells become fully integrated into the liver parenchyma over a period of several weeks (Fig. 2). Although cell engraftment may require less time, we wished to ensure that transplanted cells, which were not fractionated to exclude cytochromeP-450-expressing cells, were resistant to CCl4 injury, and the time required for this was unknown. The animals were then given three doses of CCl4 at 4-wk intervals to permit complete recovery from the preceding injury. Subsequently, the animals were observed for a total of 36 wk from the time of cell transplantation.
The first experiment utilized the transgenic G26 HBV system because serum HBsAg levels provide a simple and convenient measure of the transplanted hepatocyte mass (11). The hepatocytes were transplanted into 15 congenic C57BL/6J mice, of which 9 mice underwent CCl4 treatment and 6 mice served as untreated controls. The data showed that serum HBsAg levels increased incrementally after CCl4 treatment (Fig.3 A). In situ hybridization showed that HBsAg mRNA containing transplanted cells were present in the liver of the recipients, with an increase in the overall number of cells at the end of the 36-wk period (Fig. 3,B andC). When data were further analyzed with respect to the various time periods defined above, it became clear that serum HBsAg levels increased in each period after CCl4 treatment and were 2.7-fold greater in the final period after all three CCl4 treatments (Table1). Interestingly, serum HBsAg levels showed a gradual increase from baseline to the final period in control normal mice, which was in agreement with some spontaneous increase in the transplanted cell number without CCl4 treatment, although tissues were not analyzed to verify this.
In further studies, we performed experiments in DPPIV− F-344 rats for additional analysis of CCl4-induced increases in cell proliferation. The studies verified that transplanted cells integrated into the liver parenchyma during the baseline period of 8 wk and formed hybrid bile canaliculi by joining adjacent host hepatocytes (Fig.4, A andB). Moreover, when tissues were analyzed serially with multiple individual animals (3 rats/time point) undergoing wedge liver biopsies before each CCl4 treatment, it became clear that the number of transplanted cells in the liver increased significantly after CCl4 treatment (Fig. 4,C–E). To determine whether transplanted cells underwent DNA synthesis in this situation, three additional rats were treated with CCl4 after 8 wk of cell transplantation and were given BrdU before being killed 48 h later. BrdU incorporation was apparent in both transplanted and host hepatocytes at this time (Fig. 4 F). The proportion of cells undergoing DNA synthesis was the same in both host and transplanted hepatocytes (15 ± 4 vs. 13 ± 8%;P = not significant), indicating that the proliferative activity of transplanted cells was similar to that of the host cells.
Cumulative analysis of tissues showed 6 ± 3 transplanted cells/portal area before CCl4treatment and 15 ± 7 cells/portal area at 36 wk, subsequent to all three CCl4treatments, which represented a 2.5-fold mean increase (P < 0.001 byt-test). The analysis of cell cluster sizes was further indicative of changes in the overall cell number (Table 2). Before CCl4 treatments, the overall number of cells per cluster was limited, whereas, subsequent to CCl4 treatments, the number of cells per cluster increased markedly and large clusters containing >50–100 transplanted cells were also observed, albeit less frequently (∼1%). In addition, analysis of the position of transplanted cells in the liver lobule showed that, after CCl4 treatments, cells progressively migrated toward perivenous areas (zone 3) such that after all three CCl4 treatments, a number of transplanted cell clusters were clearly adjacent to the perivenous areas of the hepatic lobule. However, in normal control rats that did not receive CCl4, such a change in the position of transplanted cells was not observed. Also, there was no significant change in the number of transplanted cells between early or late times in control rats that did not receive CCl4. In this situation, the number of transplanted cells and cluster sizes were similar throughout to those observed at 4 wk before CCl4 administration in treated rats.
Induction of cell proliferation immediately after hepatocyte transplantation. If mechanisms could be identified for rapid amplification of transplanted hepatocyte mass, clinical applications should be facilitated. To determine this, studies were performed by transplanting cells in DPPIV− rats subsequent to CCl4 or hHGF administration. In view of preliminary data derived from pilot studies, these experiments were restricted to 2-wk durations.
Hepatocytes were transplanted into 6 DPPIV− rats (2 × 107 cells; control group), and, in 12 additional rats, CCl4 was administered 24 h before transplantation of 2 × 107 cells. Two control animals and three animals that were subjected to CCl4 treatment were each killed 1, 2, and 14 days after cell transplantation. At 24 or 48 h after transplantation in normal rats, transplanted cells appeared healthy, whereas in animals treated with CCl4 transplanted cells showed significant fatty change and vacuolization (Fig.5 A). At 14 days after transplantation, the number of transplanted cells was less in animals treated with CCl4(1 ± 3 cells/portal area) compared with normal control animals (4 ± 5 cells/portal area; P < 0.05 by ANOVA; Fig. 5, B andC). These findings suggested that residual CCl4 was harmful to transplanted cells. CCl4-induced injury to transplanted hepatocytes in portal and periportal areas was different from the absence of such an injury in fully engrafted hepatocytes. This may have been due to variable CCl4 utilization by cells because hepatocytes were not fractionated before transplantation to exclude cytochrome P-450-expressing perivenous cells.
Because a partial hepatectomy and CCl4 cause a coordinated release of a variety of growth factors including HGF (20, 21), we also investigated whether growth factor infusion at the time of cell transplantation could increase the transplanted hepatocyte mass. For this purpose, we first implanted primed osmotic pumps into three DPPIV− rats to infuse hHGF and into three additional rats to infuse saline as a control. After 24 h, F-344 rat hepatocytes were transplanted via the spleen followed by determination of transplanted cell numbers 14 days later. The animals were given 50 mg/kg body wt of BrdU intraperitoneally daily to determine whether hHGF infusion induced hepatic DNA synthesis. Although hepatocytes showed BrdU incorporation throughout the liver lobule in recipients of hHGF, morphometric analysis showed that the number of transplanted cells was not greater than that in saline-treated control animals (P = not significant).
To determine whether transplanted cells could be induced to proliferate by exogenous administration of growth factors, we infused hHGF into three mice, with a group of four control animals. The infusion was begun 24 h before transplantation of G26 HBV hepatocytes and continued for 7 days. However, there was no significant increase in the transplanted hepatocyte mass (Fig. 6).
Our findings provide novel insights into mechanisms regulating proliferation of transplanted hepatocytes in the healthy liver. The ability of transplanted hepatocytes to integrate in the liver parenchyma and assume normal polarity in the liver plate suggested to us that the cells should respond appropriately to physiological stimuli (12). We were impressed by the capacity of transplanted hepatocytes to undergo repeated proliferation in the setting of recurrent liver injury, although cell proliferation appeared to be highly regulated each time and occurred in a manner sufficient to replace the lost hepatocyte mass.
The results of our CCl4 treatment studies were particularly instructive and revealed several important features. Previous studies (27, 30) documented that transplanted hepatocytes can proliferate extensively in the setting of chronic depletion of host hepatocytes, such as in the Alb-uPA transgenic and FAH-deficient mice. Serial transplantation studies in FAH-deficient mice showed that transplanted hepatocytes can undergo multiple cell divisions while repopulating the chronically diseased liver (27). However, because transplanted cells did not previously undergo significant proliferation in the normal liver (10), it was unclear as to what mechanisms could be useful in amplifying the transplanted hepatocyte mass in the healthy liver. This consideration is especially relevant in cell therapy strategies concerning metabolic deficiency states such as familial hypercholesterolemia and Criggler-Najjar syndrome, which cause little or no liver injury and will benefit from amplification of the transplanted hepatocyte mass for superior results (1a, 7, 15, 17, 26).
Proliferation in transplanted cells commensurate with healing needed to restore liver mass after CCl4suggests that targeted hepatic ablation should be effective in applications of hepatocyte transplantation. Of course, CCl4 has extensive general toxicities as well as a relatively long half-life, precluding application, as also indicated in our studies, by injury to hepatocytes transplanted 24 h after CCl4administration. However, additional strategies might well become available in the future to ablate portions of the hepatic lobule safely. One such approach concerned transient expression ofAlb-uPA gene with an adenoviral vector in host hepatocytes before cell transplantation (39). These studies were reported subsequent to the initiation and completion of our experiments.
The migration of transplanted hepatocytes toward perivenous areas of the liver after CCl4 treatment was in agreement with shifts in host liver plates during recovery and offer further evidence for the potential of hepatocyte transplantation systems for addressing fundamental questions in liver physiology. For instance, such systems could be helpful in addressing mechanisms underlying position-specific regulation of gene expression and other events in the liver lobule that are incompletely resolved (8). We interpret migration of transplanted hepatocytes toward perivenous areas to represent a "wound repair" mechanism because such an event was not observed in the uninjured normal liver. The ability of transplanted hepatocytes to repopulate the entire liver in Alb-uPA and FAH-deficient mice, with transplanted hepatocytes shown to be present in perivenous areas (27), also illustrates replacement of injured hepatocytes and not necessarily "streaming" of hepatocytes from periportal to perivenous areas in the liver lobule, in agreement with similar interpretations by others using different systems (19).
Repeated proliferation in hepatocytes isolated from the unperturbed adult rat liver in our studies extend recent findings of extensive stem cell-type proliferative capacity in serially transplanted mouse hepatocytes (27). However, in contrast with our findings in CCl4-induced liver injury, we were intrigued to learn that transplanted hepatocytes did not proliferate in the liver after growth factor infusion, even though this induced significant hepatic DNA synthesis. Our data here are in agreement with a different proliferative response of transplanted hepatocytes after a partial hepatectomy compared with that after CCl4 treatment. Although hHGF infusion was ineffective in increasing the number of transplanted cells, we do not know whether more sustained hHGF treatment might have been effective in this regard. Similarly, whether simultaneous stimulation with additional growth factors such as transforming growth factor-α (40) or cell priming with other manipulations will improve results requires further analysis.
In conclusion, our data demonstrate that transplanted hepatocytes can be induced to proliferate significantly in the normal liver after selective injury to host hepatocytes, such as with CCl4. The role of hepatic ablative stimuli is particularly noteworthy in this respect compared with induction of DNA synthesis with growth factor stimulation. Finally, liver repopulation with genetically marked cells will permit the use of transplanted hepatocytes as powerful reporters for analyzing physiological mechanisms in liver regeneration.
We thank Dr. Ralph Schwall (Genentech, South San Francisco, CA) for the kind gift of human heptocyte growth factor.
Address for reprint requests and other correspondence: S. Gupta, Ullmann 625, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461 (E-mail: sanjvgupta{at}pol.net).
This work was supported in part by National Institute of Diabetes and Digestive and Kidney Diseases Grants K08-DK-01909 and R01-DK-46952 (to S. Gupta) and P33-DK-41296 (to D. A. Shafritz, Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, NY) and the Irma T. Hirschl Trust.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
- Copyright © 1999 the American Physiological Society


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MOVIE SYNOPSIS [ General Audiences ] Starred by Andrew W.K. (see full cast), this film directed by Nick Box and Chris Hines was premiered in TV or cinema in 2014. The feature film "Andrew W.K. Party Safari" was produced in UK. You can watch Andrew W.K. Party Safari online on TV cable or theaters with original audio in English at different dates. This film premiered in theaters in 2014.
Rating: 5 by 10 users./
Runtime: 100 minutes
Andrew W.K., Erik Payne, Chris Hines, James Balsamo, Alex Dawson, Steve Enders, Jim Hallam, James Matthews and Kris Roe.
100-minute film (full movie) directed by Nick Box and Chris Hines.
The original audio of Andrew W.K. Party Safari has dialogues in Dolby Digital, it was produced in UK.
It premiered in cinemas or television in 2014.
Peter Murfet, Nick Box, Matt Stuart, Chris Hines and Jade Mason.
This film was produced and / or financed by Hit The Floor Magazine and Dead Good Films Like Productions.
Composed by Chan Walrus, Chris Hines and David O'Neil.
Dead Good Films Like Productions.
Without commercial breaks, the full movie Andrew W.K. Party Safari has a duration of 100 minutes; the official trailer can be watched in streaming on the Internet. You can watch this full movie free in EnglishDolby Digital in movie TV channels, renting the DVD or with VoD services (Video On Demand player, Hulu) and PPV (Pay Per View, Netflix).
See the page of this full movie, Andrew W.K. Party Safari in FULLTV Movies.

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I am a former Division I Athlete and native of Portland, Oregon. I currently am a member of the team at Absolute66 Media a full service media production and creative agency that specializes in telling stories through film.
And, recently I've been helping my good friend Pierre Fassbender on his Sportystation website. Because I am also a big fan of pickleball, Pierre and I created a guide which you can find here:
This site was started at the beginning of Summer in 2007 in order to fundraise and share my experiences with the Beavers Without Borders program. You can find out more about this life-changing trip under the Beavers Without Borders (BWB) headers above.
On this site you can find a variety of videos and articles about my time at Oregon State University, my time as a Professional Basketball Player in Denmark, and about me in general.
Facebook: www.facebook.com/KevinMShane19010


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As organizations continue to leverage the Internet for business, there is an increasing demand for platforms that support a variety of different business models – including business-to-consumer, business-to-business, business-to-employee and marketplaces. Organizations are looking for common frameworks to support their interactions with their customers,… (More)
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Take the Kids to Work The Take Our Daughters and Sons to Work Foundation (TODASTW) is holding its annual national event on April 27, offering new toolkits and activity guides based on this year's theme of Dependability at DaughtersAndSonsToWork.org. The group assists businesses, families, schools and organizations throughout the year initiate their own special work day for children and mentees. Each ... Read More »
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A Veggie Leather Alternative Researchers from Iowa State University have developed a new form of synthetic leather using cellulose fibers taken from kombucha tea, along with vinegar and sugar, made in shallow plastic tanks. When a colony of bacteria and yeast is added, the material grows on the top of the liquid's surface, where it can be harvested and dried. ... Read More »
Kids Going Online at Bedtime Sleep Poorly
Issues with Electronic Use at Night A new study from King's College London has found that children's use of electronic devices close to bedtime can reduce their chances of a good night's sleep. Researchers examined 20 existing studies encompassing 125,000 children between the ages of 6 and 19. They found that youngsters using a device within 90 minutes of falling ... Read More »
The Five-Second Rule Debunked
Researchers Disprove the Thought The five-second rule is a belief that food that falls to the floor can be safely eaten as long as it's picked up quickly. Researchers from Rutgers University, in New Brunswick, New Jersey, sought to test its veracity. Four different food items were tested, including watermelon, bread and butter, plain bread and gummy candy, using four ... Read More »


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Category: Stock Vector
GraphicRiver Coffee Time 10128771
Stock Vector \ Miscellaneous
Coffee \ Available \ Shapes \ Because \ Vector \ Format \ Resize \ Resolution
Stock Vector - Coffee Time 10128771 by gokcengulenc @ GraphicRiver
Stock Vector \ Miscellaneous
Easy to edit, customize and resize, because of vector shapes. The file is available in: AI(CC), EPS (10), PSD, and high resolution 6960?×?4920 px JPG format.
Layered: No; Graphics Files Included: Photoshop PSD, JPG Image, Vector EPS, AI Illustrator; Minimum Adobe CS Version: CS.
3D- Flying Balls; Christmas Background with Present Bag; Merry Christmas Card; Happy New Year 3D Text.
Keywords: Choronemeter, break, breakfast, brown, coffee, coffee cup, concepts, creativity, cup, decoration, design, drink, elegance, energy, enjoyment, ideas, illustration, mocha, morning, mug, refreshment, steam, symbol, time, vector.


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"Those who honor me I will honor" 2 Samuel 2:30
Southern Christian Writer's Guild has met for over a decade and welcomes Christian writers of all genres. We offer educational information for writers of all levels as well as training in marketing and professional speaking. The meetings are free and open to the public. Membership is not required to participate. Dues-paying members are given the option to showcase their writings on the SCWGuild's blog and the option of a members page on the guild website to advertise their writing goals, published works, links to personal websites and contact information.
SCWGuild Northshore meets on the second Saturday of the month at Mandeville City Hall, 3101 East Causeway Approach, Mandeville LA. 70448 from 10 AM to Noon. The last meeting for 2017 is November 11th, Cherie Rickard speaking.
SCWGuild Westbank meets on the third Saturday of the month at Christian Fellowship Church, 5049 Ehret Road, Marrero LA 70072 from 10 AM to Noon. The last meeting for 2017 is November 18th, Pammela Binnings Ewen is speaking.
Enjoyed the meeting Saturday! Mimi is always a delight plus I learned something!
Looking forward to a year of learning and getting to know all of you!
Treehouse Publishers is sponsoring an author expo in Picayune MS. This is a great opportunity for writers to exhibit and sell their books as well as to get to know other Mississippi and Louisiana authors. Please visit http://msauthorexpo.com for complete details. Dr. June Knight will be live streaming personal interviews with each author.
We are very excited about this rare opportunity for dynamic exposure of talented Mississippi and Louisiana authors. Thank you for sharing this information to all who may have an interest.
So excited about the new Westbank meetings. Looking forward to great experiences.


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Mark & Lisa
Pound for Pound, you can't beat this custom built rock solid fortress of a home offered at new pricing of $105/sqft. Fantastic location with the most conveniences available - walk to the Outlet Mall, Dierbergs or any one of several excellent local restaurants. 221' of LF includes a private launch ramp, a 7' tall seawall with riprap, amazing exposed beam construction with oak doors, walnut kitchen, redwood upper deck, shake shingle roof with copper gutters and double wall stone accents thruout. A two story native stone see-thru wood burning fireplace is the focal point of the huge great room with sunken living conversation pit. A prime point lot location for all to enjoy. Tons of parking, detached garage & shop - so much for so little.


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Teach911 will help your child learn how to dial 911 from an iPhone. Teaching this valuable skill to your child could safe your life! The iPhone's keypad ...
Discontinued App
Education | Android
Educational | iPhone, iPad
Educational | iPhone, iPad
Teach911 will help your child learn how to dial 911 from an iPhone. Teaching this valuable skill to your child could safe your life! The iPhone's keypad is hidden a few clicks down and it isn't obvious to a young child how to pick up an iPhone and dial a number. It's important for every parent to show their child how to get to the key pad, dial 911, and how to recite their name and address for emergency workers. Teach911 has 2 modes: Assistance and Advanced. - Assistance mode highlights the correct sequence of buttons to click from the home page, the phone app, and all the way to dialing and hitting call. A few unsuccessful clicks will then re-show the highlight arrow to pinpoint the correct button again. - Advanced mode has no highlighting and requires your child to get to the phone app, the keypad tab, and then dial 911 and hit call all by themselves. Use this mode as a final challenge to make sure your child understands what to do. Once your child calls correctly Teach911 will ring a couple of times and then a 911 responder will ask for the three things every child should be taught to be able to say in an emergency: - The Emergency - Their Name - Their Address Finally, they'll get a loud hooray and the 911 responder will tell them great job. Other Features: - Contains a red and white border so you can tell the difference between the real phone application and Teach911 - Let's your child practice calling 911 without really calling (911 operators tend to dislike people calling for practice) - Provides a fun way to for you to teach an otherwise dull and scary subject to your child. * Please remember to remove this app once you are done teaching your child so it can't be confused in an emergency.
Version: 1.2
Size: 3.13 MB
Price: 0,00 €
Developed by TAGn Solutions
Day of release: 2010-06-26
Recommended age: 4+


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women screaming while giving birth (this is why I'm not having kids)
Description:Hi Guys!!! This is a montage I created of a bunch of women screaming while giving birth and I'm warning ya those women know how to scream. In this video those women were screaming extremely loud so turn down your speakers. Thank God that I don't have children so I'll never go through that and plus children are brats so no kids for me.
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"A Balance Model of Self-Regulatory Failure"–SAGE Center Lecture by Todd Heatherton
Free Event
Todd Heatherton is the Lincoln Filene Professor in Human Relations in the Department of Psychological and Brain Sciences at Dartmouth College. His research takes a social brain sciences approach, which combines theories and methods of evolutionary psychology, social cognition, and cognitive neuroscience to examine the neural underpinnings of social behavior. He was elected President of the Society of Personality and Social Psychology in 2011 and has served on the executive committees of the Social and Affective Neuroscience Society, the Association of Researchers in Personality, and the International Society of Self & Identity. He is a Fellow of the American Association for the Advancement of Science, the American Psychological Association, the Association for Psychological Science, the Society of Experimental Social Psychology, and the Society for Personality and Social Psychology.


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So, here is the deal.
I have spent labor day setting up my Linux-based network, and have been stuck for a few hours on this:
I have a Window$ PC from which I would like to transfer data to my Linux box. I plan to do this via SSH (using putty from my Window$ machine).
I have connected both ethernet cards together (Linux Box - To - Windows Box), and I am trying to configure them as a LAN.
So, after searching the Internet I have tried:
# ifconfig eth0 192.168.1.2 netmask 255.255.255.0
# route add default gw 192.168.1.1 eth0
And then setting the Window$ Box to:
Subnet Mask: 255.255.255.0
Default Gateway: 192.168.1.1
DNS Server: 192.168.1.1
I know this was probably stupid to do, so if you can spare the time (as I am sure it takes less than 1 min to write a fix for this) I ask for your help in setting this up.
Thanks in advance.
Edit: I forgot to mention, that I do not want to setup DHCP or anything, I just want to transfer files (too big to put in any portable device). Thanks.


text:
industrial use of ball mill
industrial ball mills for manufacturing glaze usa,MTM Crusher
Cement Grinding Ball Mill For Sale Price And Manufacturers,stone . 13 May 2014 Email Us,Free Inquiry. The Cement Ball Mill is a high efficiency fine grinding machine that widely used in the industrial production and select mine . especially used in cement , silicate , new grind glass, powder ...Read More
Used Ball Mills,Machinery and Equipment
Machinery & Equipment buy and sells Used Ball Mills, industrial and commercial ball mills including ball mills, pebble mills, SAG mills, rod mills and more. View our online inventory and send your us your quote request.Read More
The grinding media obtained from the industrial ball mill were sized and visually examined for deviation from the initial spherical shape. The 2D plane ball shapes photographed using a camera, were used to represent the shape of the media.Read More
Industrial use ball mill for cement making prodcution line ...
Industrial use ball mill for cement making prodcution line,US $ 5,000,58,000 / Set, New, Ball Mill, 1-50 t/h.Source from Henan Bailing Machinery Co., Ltd. on Alibaba.com.Read More
Used Ball Mills,Sag Mills,Rod Mills
Used Ball Mills, industrial and commercial ball mills including pebble mills, sag mills and more.Read More
Ball mill,Wikipedia, the free encyclopedia
The ball mill is used for grinding materials such as coal, ... The general idea behind the ball mill is an ancient one, but it was not until the industrial revolution and the invention of steam power that an effective ball milling machine could be built.Read More
Ball mills, Ball milling-cutters,All industrial ...
A ball mill crushes solids into a fine powder within a rotating drum, using balls as the grinding medium. Applications. Ball mills are found in settings as diverse as laboratories and mines, among others.Read More
used copper ore processing equipment & ball mill grinder ...
Silver Ore Processing Equipment marblecrusher . it has been common economic practice, although plenty of used copper are deserted.The selection of silver ore processing equipmentRead More
industrial use of ball mill
Ball mill ,Wikipedia, the free encyclopedia HTTPS Preferred® This result has been upgraded by HTTPS Preferred® to an HTTPS link, which protects your privacy against snooping.Read More
salt lake city used industrial equipment ball mill
SHANGHAI NMN MACHINERY CO., LTD is one high-tech enterprise, which involves R&D, production, sales and service as well. In the past 20 years, we devote to producing mining equipments, sand making machines and industrial grinding mills, offering expressway, rail way and water conservancy projects ...Read More
Industrial Mills,Ball Mills Batch Type, Ball Mills ...
Manufacturer and Exporter of Industrial Mills, Ball Mills Batch Type, Trapezium Mill, Ball Mills Continuous Type, Vertical Roller Mill and Pug Mill offered by SRI SAI HEAVY MACHINERIES, Rajapalaiyam, Tamil Nadu, India. Send Email. Send SMS. Search. Home: Profile:Read More
Mill (grinding),Wikipedia, the free encyclopedia
... one example being the Sepro tyre drive Grinding Mill. Industrial ball mills can be as large as 8.5 m (28 ft) ... SAG mills use a ball charge of 8 to 21%. The largest SAG mill is 42' in diameter, powered by a 28 MW (38,000 HP) motor.Read More
kvs ball mills, mill all industrial manufacturers in,SBM ...
kvs ball mills, mill all industrial manufacturers in this iron heritage festival blast furnaces, forges, foundries, and rolling mills new york city to. ... a kvs ball mill is used in the manufacture of lime products at tenn . luttrell. the kvs ball mill is a 450 hp, ...Read More
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industrial ball mill capacity. Daniel Augustus Tompkins, 18511914. Cotton Mill Cotton Mill, Commercial Features. A TextBook for the Use of Textile Schools and Investors.Read More
Ball Mill,Grinding Mills,Ball Grinder,Grinding Ball ...
Ball mill is ore dressing equipment, mainly used for crushing bulk ore materials into ore pulp, ... Ball mill machine or industrial grinding machines can be divided into overflow grinding ball and grid grinding miller according to discharging way.Read More
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3 Lb Capacity Industrial Ball Mill ,Crusher USA » 3 lb capacity industrial ball mill » 6 lb. capacity ball mill australia » 3 lb capacity ball mill » what coal mill, vertical roller mill, raw mill, ball mill etcRead More
used industrial ball mill & hammer crusher equipment
Ball Mills Used Mineral Processing Equipment / Used Ball Mills. Find used milling equipment for sale. Call us at: 7056712100. Ball Mills Featuring: Dominion Engineering, , Allis Chalmers, Gardner Denver, Marcy, DenverRead More
Ball mill grinder|Ball machine|Industrial grinding machines ...
Overview of Ball Mill Grinder. Ball mill or ball mill grinder is one of the most widely used superfineindustrial grinding machines in mining industry.Read More
Grinding media steel balls, hardened steel media, 2 to 12 mm.
This steel grinding media for ball mills, pulverizers and attritors is cold forged and ground. The hardened steel balls (shots, beads) are available in different hardness, as required, in chrome alloy steel. ... Ball Mill used for grinding, milling, dispersionRead More
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Our organization is one of the trusted manufacturers and suppliers of industrial Ball Mills that comply with international standards.Read More
Types of Ball Mills,eHow
Like all ball mills, the speed and ball size have an effect on how the vertical ball mill works. Vertical ball mills often come with a variety of ball sizes (and have variable speed settings) ... What distinguishes the industrial ball mill ...Read More
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Results 1 to 3 of 3
Thread: Create
07-02-09, 00:01 #1Registered User
- Join Date
- Jan 2009
- Posts
- 4
Unanswered: Create
I need to create a UAT environment. Only one server is available to me and I already have the development database on it (say Mydb), so I have to create the UAT database with another name (say Mydb_UAT).
What is the simplest way to do it ?
Can I do it using 'load database' command ? In the manual I cannot find the syntax to load a database with a different name.
Thanks for the help in advance.
07-02-09, 03:34 #2Registered User
Provided Answers: 1
- Join Date
- May 2005
- Location
- South Africa
- Posts
- 1,369
You can load using a dump from any db of the same size
07-03-09, 04:00 #3Registered User
- Join Date
- Jun 2009
- Posts
- 25
U can create a database by using the create database with load option and when u create a database check Mydb size and create one "Mydb_UAT" with similar size and load the database mydb in mydb_UAT.
suppose if your database name dump name is /backup/mydb.dmp then u can load as
1>load database mydb_UAT from "backup/mydb.dmp" 2>go


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Shaun White is spectacular, but the chewing gum mintacular is not. It warms anyone's heart to hear the story of Shaun White overcoming a heart defect and eventually become an Olympic athlete. Mintacular, however, is just ANOTHER spearmint and melon artificially flavored chewing gum. The flavor reminds me of a cleaning compound or a shampoo, it is very cosmetic. The aqua color seems a bit cosmetic as well. A current trend is spearmint gum is to combine it with an artificial melon flavor. Spearmint gums from Stride, Trident and Mentos all have this flavor blend. Wrigley 5 Rain and Ice Breaker Ice Cube spearmint gums have an authentic spearmint flavor. Spearmint and melon flavored gums on the market are reliant on synthetic l-Carvone … [Read more...]
Stride Shaun White mintacular artificially flavored sugar free chewing gum
Trident Layers Juicy Berry & Tangy Tangerine sugar free gum, flavor review
Trident Layers gum is my daughter's favorite gum. She loves the juicy, fruity flavor and the colored layers. She calls it the "sandwich gum" and jumps up and down when I give her a piece. The front label of Trident Layers reads "with real fruit flavor". What does that mean? Sugar free chewing gum is not natural. Sugar free gum is an artificially flavored, sweetened, and colored edible rubber material. Its benefits are dental (does not promote tooth decay) and breathe freshening. The ingredients in Trident Layers are: gum base (rubber) maltitol, maltitol syrup, sorbitol, mannitol(sweeteners that do not promote tooth decay) acesulfame potassium , aspartame, sucralose (artificial high potency sweeteners) Blue 2 … [Read more...]
Wrigley's 5, Cobalt cooling peppermint sugar-free gum now in a jar
Wrigley's 5 Cobalt cooling peppermint sugar-free gum is an extremely popular gum. I had several requests to copy this flavor when I was in the industry. Wrigley decided it was popular enough that it would need a dragee form (candy coated pellet gum) and a jar. Thank you Wrigley! The taste of the candy coated pellet gum is a little different from the stick form of Wrigley's 5 Cobalt. The pellet form has much more initial cooling character, less sweet and slightly oxidized (pine needle character). It's still a great gum to freshen your breath, but harsher than the stick form of Wrigley's 5 Cobalt. I'm interested in seeing Wrigley's 5 Rain (Spearmint) in a jar as well. Rain is one of my very most favorite gums. … [Read more...]
Extra Dessert Delights Sugar Free Gum, Root Beer Float Flavor
Root beer floats are a favorite summer treat in the United States. Believe it or not, root beer is not enjoyed by all cultures and countries. Native Americans introduced root beer and made it using birch bark or wintergreen(characterized by methyl salicylate). http://tiny.cc/mp4ogw. Most of the world associates the taste of wintergreen with medicine or bathroom cleaner. Americans enjoy wintergreen and root beer flavor in their sweets. Personally, it's difficult for me to imagine not enjoying root beer. In order to provide a proper evaluation of Extra Dessert Delights Root Beer Float gum, I decided to start with a home made root beer float gold standard (vanilla ice cream and root beer). Root beer floats are cool, creamy, sweet, tingly, … [Read more...]
Stride Tropical Trance, artificially flavored sugar free gum, Flavor Review
Be careful,because Stride Tropical Trance will hold you captive for a half hour. Tropical Trance has high flavor impact that lasts at least 20-30 minutes. This gum is shocking: high in sour, sweet, tropical flavor and cooling sensation. I'd describe the flavor as a very creamy mango with orange, pineapple, green cucumber and floral notes. The creamy notes are very high and I much prefer this gum after those flavor notes have dissipated. Twenty minutes into chewing Tropical Trance, I find it to be a delicious fruitty berry gum that is cooling. Since I've spent almost 20 years in the flavor business , I do not really like highly flavored food items. I feel I can taste the individual flavor aromatics. In this case it is a lactone, perhaps … [Read more...]
Wrigley's 5 and Orbit new small pack (6 pieces)
Aren't these cute? Recently I found these little cuties on a recent trip to Walmart. I don't think I'd be a regular customer, but it's nice to have an option. … [Read more...]
Extra Dessert Delights Rainbow Sherbet flavor review, limited edition
About six weeks ago, I heard Extra launched a limited edition dessert delights rainbow sherbet flavor; I couldn't find it anywhere and I was frantic. Finally, I saw it at Target in a three pack. Extra has developed a great new line of gum,dessert delights, using the most current flavor technology, sensates. Sensates are flavors that contribute cool sensation without minty or off flavors. Therefore, a flavor scientist can blend sherbet flavor with a sensate and offer the consumer a refreshing, sweet gum that is cool and not minty. Menthol (the characterizing part of peppermint) and sensates affect the body's thermal receptors by stimulating ionic channels. Our body also feels cool through evaporation. Rainbow sherbet flavor reminds me … [Read more...]
Project 7 sugar free Fresh mint gum, flavor review
I'm a sucker for helping others on a global scale. Usually, I consider help writing checks to reputable organizations. Do I think buying gum will help the world? Not really. Especially this gum. There is nothing unique about Project 7 gum and it tastes bad, like it's been a hot car all summer. Will Project 7's goal of one million new fruit tree plantings in Guatemala, El Salvador and Nicaragua change much? Maybe...I'm not certain. I'd rather write a check, though, than chew this gum. When I first pop this natural & artificially flavored, artificially colored and synthetically sweetened gum in my mouth; my impression is pinesol and dirt! I imagine the mint oils sourced for this gum are from foreign countries and unprocessed. Very … [Read more...]
Trident Layers Orchard Peach & Ripe Mango flavor review
As a flavor chemist, I have to applaud the flavorist who made this peach & mango flavor. This is a well-balanced, creamy, tropical, crisp, fruity peach mango flavor and would be very difficult to copy. As a consumer and mom, I am concerned about such high flavor load in a product that children would consume. Typically fruit flavors are a "top note" for beverages or fruit fillings that lose some potency during processing. In confection, though, added fruit flavors are the entire flavor system. Chewing gum in particular has an extremely high flavor load, perhaps 2% vs a piece of candy that has 0.2%. Flavor load is high because it is desired that gum's flavor last 20 minutes. The United States government is very strict about what … [Read more...]
Orbit wildberry remix sugar free chewing gum review
Often I hear "our world is getting smaller". When people say that; they are referring to the ease of travel or transportation. How about food and flavor? When I visited Italy 10 years ago, a very popular confection & beverage flavor was forest fruits. Forest flavors is portrayed with pictures of blackberries, blueberries and raspberries and is always somewhat floral. My first impression of the Orbit wildberry remix is that it's forest fruits flavor from Europe. When I opened the packaged and smelled the gum, though, I changed my mind. Orbit Wildberry Remix smells just like grape soda or grape candy flavor for kids. Initially, the gum was juicy, sweet and flavorful. It reminded me of a berry flavored Starburst, and I love Starbursts. … [Read more...]


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New World Order Real or Conspiracy
Started by Bruceafc, Mar 13 2012 11:51 PM
2 replies to this topic
Posted 13 March 2012 - 11:51 PM Edited by Bruceafc, 13 March 2012 - 11:57 PM.
Could someone please make the youtube videos viewable hear instead of just links
Posted 14 March 2012 - 01:24 AM
I don't understand the question. Are you asking if the new world order is real or if it is a conspiracy by people to make it seem real? The answer is obviously yes.
Posted 14 March 2012 - 03:24 PM
There are minimum standards topics must meet in order for them to qualify for this section of the forum. This topic meets none of them. And that's quite aside from the fact that a) NWO has been discussed extensively already, and b) it's unadulterated bollocks in every way.
1 user(s) are reading this topic
0 members, 1 guests, 0 anonymous users


text:
- Price
- $10.00
- Pages
- 108
- Formats
- PDF EPUB Mobi
- Version
- 3.1
- Updated
- Dec 06, 2007
Take Control of Buying a Digital Camera, Third Edition
Find practical, real-world advice that helps you choose a digital camera!
Rather than bury you with arcane technical details or a myriad of camera models, Larry first helps you determine how much you want to spend, what sort of pictures you're likely to take, and what aspects of using a camera are important to you. With that grounding, you're ready to learn about the different camera features, separated by those that are actually important and those that merely fill up feature checklists. The ebook even contains a camera comparison worksheet you can fill in while shopping. Additional sections give you pointers on how to read camera reviews (plus a list of the best review sites!), advice about evaluating picture quality, suggestions of where to buy your camera, the lowdown on accessories you might want, and tips on working with photos on your computer.
"I wrote this book because I was frustrated that nobody reviews cameras or gives camera buying advice based on how working photographers actually use cameras to create pictures. The camera is ultimately just a tool, one that either helps or hinders the photographic process." —Laurence Chen
- Update Plans
January 2, 2008 – We have no plans to update the text of Take Control of Buying a Digital Camera in the near future.
Posted by Adam S Khan


text:
Back home in Franklin, Mississippi Valley State wide receiver Paul Cox had discipline instilled in his household at an early age.
After all, his dad, Paul Cox Sr., is a master sergeant in the U.S. Army and his mom, Monica Cox, works at the clerk of courts for the St. Mary Parish court house.
"They always talked about discipline, discipline, discipline," Cox said. "When I was younger, I never understood why my family was so hard on me."
It was his family's discipline that molded him into the man and football player he is today, Cox said.
At seven years old, he recalls playing flag football in Morgan City, where his grandfather constantly told him to never quit, never stop, and to be the fastest person on the field.
After playing at Patterson High under Timmy Minton, Cox decided to play his college football outside of Louisiana.
Despite interest from schools like Louisiana Tech, Nicholls State and Louisiana-Lafayette, Cox turned to Mississippi Valley State, in Itta Bena, Miss.
"It was great experience being at a local school," Cox said. "The fans were always great, and the stadium was always packed. But me and my family thought Mississippi was the better option. It allowed me to go out and explore."
Since arriving on-campus for the 2008 season, Cox and the rest of the Delta Devils have struggled to win. Over that span MVSU is 6-29 - with only two Southwestern Athletic Conference wins. They'll try again Saturday as Southern visits.
Last season the team couldn't even play at their stadium after it was deemed unsafe. The Delta Devils had to travel around 45 minutes for every "home" game. Cox said the local support has been tough, especially considering that MVSU is the smallest school in the SWAC.
So far this season at Rice-Totten Stadium, the team has pulled in a combined attendance of 5,989 in two home games.
However, even with the team's struggles, Cox remains a bright spot for the Delta Devils.
"Cox has done a good job for us," Mississippi Valley State coach Karl Morgan said. "He's a talented guy, and he's a good guy off the field."
As a junior last season, Cox finished with 21 receptions for 520 yards and five touchdowns - leading all SWAC receivers with an average of 24.8 yards per catch. Now a senior and offensive team captain, Cox is already closing in on his production from last season in just four games.
Cox is currently second in the SWAC in receiving yards (323), third in yards per catch (17.9) and sixth in receptions (18).
The 6-foot-5, 205-pound receiver - who said he runs a 4.5-second 40-yard dash - had the most complete game of his career last week, Morgan said, in a 43-34 loss at Prairie View. Cox caught six passes for 137 yards and two touchdowns.
"He's the guy that really is our go-to guy on offense," Morgan said. "We definitely will try to get the ball to him, and he makes things happen after he gets it."
With self-described explosiveness, and NFL size and speed, more than half of the NFL's teams have sent scouts to watch Cox practice, including the Buffalo Bills and New Orleans Saints, Morgan said.
The last time a Valley player was drafted by the NFL was 1992, when the Indianapolis Colts selected cornerback Ashley Ambrose in the second round and running back Ronald Humphrey in the eighth round.
It's a long that many college athletes don't experience. But with discipline and skills, Cox may get a shot.
"That's my dream," Cox said. "That's my ultimate dream, but I have a lot of work to do to get there."


text:
Quote of the Day
"I just don't really
understand how you
can see it as a bad thing."
-- Rangers and Sweden G Henrik Lundqvist, on the NHL participating in the Olympics (N.Y. DAILY NEWS, 3/2).
Top Stories
Getting Some Fresh Air
Jordan Set To Become
New Bobcats Owner
While most of the sporting world is transfixed over the weekend by the conclusion of the Vancouver Games and the scintillating Canada-U.S. hockey game, Bobcats Owner Bob Johnson agrees to sell the team to Michael Jordan. The price Jordan and his team of investors are paying is up to some debate, but it seems certain that Johnson will see a loss from the $300M he spent for the team in '03. Jordan's increased involvement could provide a sense of stability for the young franchise, which has struggled to gain a foothold in the Charlotte market. There already are calls that Jordan must maintain a larger presence around the team in this new role. Meanwhile, at least one local columnist is saying the team should take the ownership change as an opportunity to get rid of the "Bobcats" moniker, which has been "irreversibly tainted by its association with Johnson, who more or less named the team for himself."
The Final Score
NBC earns a healthy 13.8 final primetime Nielsen rating for Vancouver Olympic coverage.
Time Is On Our Side
While Gary Bettman says "there's plenty of time" to make a decision on Sochi, pundits say NHL would be "incredibly stupid to not play."
Gone But Not Forgotten
David Freeman steps down as Chair of Predators amid growing scrutiny about his tax problems, but he plans to keep the largest stake in the club.
Having Pitch Problems
MLS delays the roster compliance date as it continues to operate without a CBA.
A Friend Of The Devils
A strongly funded group of ManU supporters discuss $1.5B bid to buy out Glazers.
The Joy Of Six
LeBron lets the NBA know his plan to switch from No. 23 to No. 6 next season is for real.
Basketball & Taxes?
The Nets' latest unique sponsorship effort will offer free tax return preparation to fans.
Big Whiskey And The UK King
Maker's Mark releasing limited-edition bottle featuring the likeness of John Calipari.
- NBC Averages 13.8 Rating For Olympics
- Bettman Patient About '14 Decision
- U.S. Medal Haul Sign Of Power Shift
- Vancouver Games Continue To Be Praised
- Reno, Lake Tahoe Want To Host '22 Games
- Olympians Could See Boost After Tiger's ...
- VANOC Sees Windfall From Mitten Sales
- Do Olympic Ads Need Warm Weather?
- Olympic Notes


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What is Chye Koey?
Chey Koey is basically cook jullian turnips encase in rice dough which is similar to those use in Har Kau a type of shrimp tim sum, another variation is with Ku Chai, a type of green vege similar to spring onions.
In a small town of Sungai Bakap (a 2 street town) on Penang Mainland (Seberang Prai Selatan), there are two restaurants where this simple dish is serve. So well know is this dish here that patrons from Bukit Mertajam and Butterworth (a distance of 50km one way) will travel here to have this dish for breakfast.
Well due to this recommendation, I have to give this dish a try. As inform there are two restaurant. On Wednesday one of them was close as such which restaurant i am to try was already chosen for me.
On the Northern end of the Old Town, along the Main road, (Note : if you see the hospital, reverse to the other end of the town. is the area where the Chye Koey is Located. The Restaurant is Named : Soon Lee Hou. Picture provided, and can be easily identified by the extra tables put out. The other Restaurant is located opposite this one and seen not to have a name.
As it was a wednesday morning, it was quite and my family was quickly attended to on our arrival. Our Tea order was taken, and we were ask if we wanted to order any soup, fish, noodles or not. Mind you this is a 8am in the morning. Looking around we realise that most of the table have indeed order this items along with some tim sum.
I ordered a Hokkian Char, the Penang version, not the KL version and ask them to bring out the Tim Sum, and the chye koey. What came out were not what you will get in big city restaurant. The tim sum here is not dainty pieces. What you get however are tasty pieces of food and limited to only a few dishes.
First off there is the stuff crab, we took 2 plates as my eldest daughter love it.
The there were the Chye Koey, we ended with 4 plates, 2 plates the Ku Chye type of chye koey, 2 plates of siew mai, and 2 plates of fried tim sum.
and one plate of Hokkian Char,
Interior shot of the Restaurant, the cooks were busy preparing more chye koey.
The Food is fresh and tasty. Total bill came up to RM27 for the 3 of us + one baby. The Chye Koey, well It is nice, especially the "Ku Chye" Chye Koey, which to me was excellent and aromatic. My wife prefer the standard chye koey, But I find it too mushy (soft).
Would i want to travel 50km one way for this only? Well no.
Would i want to drop in if i am travelling this way? Yes I would.
Who come here for Breakfast? Well i was inform that businessman from Bukit Mertajam, Butterworth, Local, and Golfers (Playing at Krystal Golf, and Bukit Jawi Golf Resort) will have their breakfast here.
In addition I notice one thing here at Sungai Bakap, of the 10 chinese base restaurant / coffeeshop in the area, 5 were selling roast duck at 8am in the morning. I am sure that there is a story behind that as well.


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Please help, my computer well, Chrome crashed last evening at about 1 am when I was about to begin a Hub after emailing a friend to chat. I am not knowing what to do. I uninstalled it, could not catch the messages I got and reinstalled twice and am not sure about all the sync things with Google, I had it all set up until this crash and burn...had 1 hour sleep in 24...going on fumes now!!!! This barge is sinking quick!!!
sort by best latest
I'm not a computer geek--you should know that first off. However, my husband and I each switch to using Chrome last year, from IE. In the last month or two, the function of Chrome deteriorated. It got bad enough that we both switched back to using IE. I was pleased to find IE can now do some things it could not do and had caused me to swtich to Chrome in the first place. Also, after serveral days and a couple reboots of the computer, my IE internet windows function so much better than in Chrome. They are not slow, like Chrome had gotten to be, nor are they getting hung up very much--not near as much as in Chrome. This does not address your actual question, but just in case it applies, I'm sharing our experience. Hope you get the help you need and catch up on the lost sleep!
You can help the HubPages community highlight top quality content by ranking this answer up or down.
Thank you for your concern and suggestions, especially experiences. I am still not certain, I know I took the browser off and it was almost an immediate reaction after the crash since Flash player had been acting up. Will keep you posted:)
Chrome works fine in my system. I just don't like it creating too much processes when you open new tabs. Hard to compute RAM usage.
Anyway, the answer is system restore. restore your computer to an earlier point/time where Chrome was still working. Unless there are no recent restore points or the others are too far way back. It's worth trying. It can be reversed anyway.
What is your OS?
on XP type "rstrui" on run
on 7 type "restore" the start menu
P.S. I'm not a computer geek
You can help the HubPages community highlight top quality content by ranking this answer up or down.
Operating system is Windows Internet Explorer 7+9 64-bit, and I hate to tell you but at around 4 a.m. I was doing a system restore and it would not go, could not find a restore point when the chrome was still installed. aw snap, I was warned!!
that's bad. can you tell us more on how it crashed? error messages if any? The best thing you can do in the meantime is use another browser.
Using registry clean up software might do the trick. it could be errors in the registry.


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The Death's Head Chess Club tells the story of an unlikely relationship between a Jewish Auschwitz survivor and an SS officer. The officer, Paul Meissner, sent to Auschwitz after being badly wounded, is put in charge of SS morale. He starts a chess club, which thrives because of gambling on the games. The survivor, Emil Clément, is a gifted chess player.
In Auschwitz, Emil's children are sent to the gas chamber and he is forced to become a slave labourer. Each day he endures starvation, indignity and brutality under the constant threat of the gas chamber.
In a chance conversation, Meissner learns that chess is played by the prisoners, and that one of them is said to be unbeatable. The idea of an unbeatable Jew is an affront to SS sensibilities, and a challenge is issued.
At first, Emil refuses to play for the gratification of the SS. Eventually, he agrees to play because, if he wins, a prisoner's life will be spared from the gas chamber. In the games that follow, Emil's victories infuriate the SS.
The story moves between 1944 Auschwitz and 1962 Amsterdam, where Emil is participating in an international chess tournament. Here, Emil encounters Meissner, now a Catholic priest, who has terminal cancer. Meissner carries his SS culpability endlessly. He is determined to become reconciled with Emil before he dies.


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About Our Yoga Classes
Hatha yoga weaves together body, mind, and breath into a practice that teaches us to live from the depth of our own wisdom; in harmony with our total environment. Its aim is a healthier body, an adaptable mind, emotional clarity, and a connection to the stillness at the heart of our being.
The Movement Center's yoga studio offers a core yoga curriculum where asana, breath, inner awareness, and meditation are equally important. Students are encouraged to use breath and awareness to release tensions from their mind and body so that the body may open to the deeper source of healing and wisdom from within.
We work to honor the natural intelligence of the practice, the body and the spirit of each individual. Classes are inclusive and refined. All ages, body-types, and abilities will find a class on our schedule suitable for them. We also offer several high-quality $5 yoga classes for those with limited budgets. No matter where you come from, you will find something here for you.
Our classes are unique and unlike anything else offered in town. Set in the profoundly nourishing environment that is our very special grounds - it is no small exaggeration to say that each class is a deeply healing experience for each student - every time.
Choosing a Class
If you are just beginning your yoga practice, we'll help you get started with our 2-week Beginners Series. If you have an established practice, a good way to choose a class is to read its description in the schedule and then the teacher's bio page. And, in the time-honored tradition of hatha yoga, we offer individual instruction for anyone at any point in their practice.
Anytime you want to be in touch about a class you can email us at firstname.lastname@example.com
Give time and devotion to your yoga practice and it will transform your life into an unimaginable possibility!


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Flex Level Popup Menu
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Flex Level Popup Menu Hosting Promo
Enhance arbitrary links on your page with some multi level powers with Flex Level Popup Menu! It lets you associate a multi level drop down menu to any link on the page, so moving the mouse over the link activates the menu to be shown beside it. Each pop up menu is simply defined as a regular nested UL on the page, making it very intuitive to set up, not to mention the menus search engine friendly!Visit publisher site: Flex Level Popup Menu
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|Johannesburg Tour
The Jo'burg city tour includes scenic views of the City of Gold, historic sites, Top of Africa - Africa's tallest building, Mandela Square, Mandela home, Traditional medicine market specialising in African herbs, Maboneng the newly developed part of Johannesburg & Braamfontein markets, Newtown, Hillbrow - Jozi red light district and a stop at the artsy and multicultural suburb called Mellvile to mingle and engage with various locals and global expats. A stop for lunch on request is optional.
Time:9 00 am - 1.00 pm or 1.00 pm-5.00 pm
Price: ZAR R650.00 pp (incl pick & drop off at selected pick up spots. Please enquire on areas)


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Separate names with a comma.
Sign up and join the fastest growing Yuneec drone community.
I've had the Typhoon 4k for a couple of months now and I love it. Easy to fly and very smooth. This is my first video edit from a...
We just launched part of our drone pilot job board on 9/1 ( www.drones4hire.com ). We want to expand on this platform with the drone pilot in...
My last flight with my Typhoon G - which is now unfortunately sold.
Aerial footage with GoPro Hero4Black.
Appreciate your feedback,...
Few shortcuts from my training flight in my hometown Düsseldorf, Germany.
Comments are appreciated.
Cheers, guys!


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La'Darius Newbold
Position: Cornerback
Ht./Wt.: 6-1, 201
Class-Exp.: So.-1L
High school: Lancaster
Last year: Newbold made his career debut, getting into three games and making one tackle. Tech lost seniors Bruce Jones, Olauluwa Falemi and Derrick Mays, who accounted for 23 starts last year (out of a possible 26) at the the two positions. In the spring, Newbold jumped into the wide-open competition to replace them.
Outlook for 2014: Though Newbold was listed first team coming out of spring, no corner was given any real assurance of being in the two-deep, other than possibly Justis Nelson.
Now nine days from the season opener, coaches have offered little definitive insight about the cornerback spots other than this: Nelson still looks like the No. 1, sophomores Newbold and Thierry Nguema are in the mix and true freshmen Tevin Madison and Jah'Shawn Johnson are trying to seize the opportunity to play.
Newbold was highly recruited before he signed in 2012, but he was raw technique-wise. With his high-cut build, Newbold's more of a long strider than a short-space quickness guy, so defensive coordinator Matt Wallerstedt gave some thought to putting him at Raider outside linebacker.
Newbold has plus size and runs well, but there's more to being a Big 12 cornerback than that. Paying attention to the mechanics of the position and not dropping his head when he gets beat are his keys going forward.
"He's gotten better," corners coach Kevin Curtis said Wednesday. "He's playing with better effort and better technique. For him, like everybody else, just continue to make plays and make more plays."


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First Time Caller, Longtime Listener, It's Dr. Robotnik
Sonic's arch-nemesis took time for a brief call to morning radio jocks Opie & Anthony to talk about evil. It's a short clip but a great impersonation, and the duo's reactions are worthy a chortle or two. They're also NSFW, so keep the volume down if there are tender ears nearby.


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Provides an adapter layer between core routines for converting
to and from UTF8 and other encodings. In essence, a way to give
multiple existing Unicode modules a single common interface so
you don't have to know the underlaying implementations to do
simple UTF8 to-from other character set string conversions.
As such, it wraps the Unicode::String, Unicode::Map8,
Unicode::Map and Jcode modules in a standardized and simple
WWW: http://search.cpan.org/dist/Unicode-MapUTF8/
make generate-plist
To install the port: cd /usr/ports/converters/p5-Unicode-MapUTF8/ && make install cleanTo add the package: pkg install p5-Unicode-MapUTF8
cd /usr/ports/converters/p5-Unicode-MapUTF8/ && make install clean
pkg install p5-Unicode-MapUTF8
PKGNAME: p5-Unicode-MapUTF8
SHA256 (Unicode-MapUTF8-1.11.tar.gz) = db534d74b18b2464743efc6aada51854e0fde88f875d208a154eddb9f2b50e93
SIZE (Unicode-MapUTF8-1.11.tar.gz) = 16402
NOTE: FreshPorts displays only information on required and default dependencies. Optional dependencies are not covered.
This port is required by:
No options to configure
Number of commits found: 23
Remove ${PORTSDIR}/ from dependencies, Mk and categories a, b, and c.
With hat: portmgr
Sponsored by: Absolight
Reassign converters/ perl modules to perl@.
- Sort plists
- Fix p5-Convert-BER's bizarre pkg-descr
- p5-Unicode-Map8 fails make test (and did so before)
- p5-XML-WBXML fails make test (and did so before)
Change the way Perl modules are installed, update the default Perl to 5.18.
Before, we had:
site_perl : lib/perl5/site_perl/5.18
site_perl/perl_arch : lib/perl5/site_perl/5.18/mach
perl_man3 : lib/perl5/5.18/man/man3
Now we have:
site_perl : lib/perl5/site_perl
site_arch : lib/perl5/site_perl/mach/5.18
perl_man3 : lib/perl5/site_perl/man/man3
Modules without any .so will be installed at the same place regardless of the
Cleanup plist
Support STAGEDIR.
Add NO_STAGE all over the place in preparation for the staging support (cat:
- Convert to new perl framework
- fix p5-Jcode issue
- Remove SITE_PERL from *_DEPENDS
At the moment 1385 ports use BUILD_DEPENDS= ${RUN_DEPENDS} and 450
ports use BUILD_DEPENDS:= ${RUN_DEPENDS}. This patch fixes ports that are
currently broken. This is a temporary measure until we organically stop using
:= or someone(s) spend a lot of time changing all the ports over.
Explicit duplication > := > = and this just moves ports one step to the left
Approved by: portmgr
End WWW CPAN URL with a "/"
- Get Rid MD5 support
For ports maintained by email@example.com, remove names and/or
e-mail addresses from the pkg-descr file that could reasonably
be mistaken for maintainer contact information in order to avoid
confusion on the part of users looking for support. As a pleasant
side effect this also avoids confusion and/or frustration for people
who are no longer maintaining those ports.
- lang/perl5.6 is dead, remove PERL_LEVEL/PERL_VERSION < 500801 checks
PR: ports/135398
Tested by: 2 -exp runs by pav
Approved by: portmgr (pav)
- Take advantage of CPAN macro from bsd.sites.mk, change
${MASTER_SITE_PERL_CPAN} to CPAN.
PR: ports/122674
Submitted by: Philip M. Gollucci <email@example.com>
Reworked by: araujo (myself)
Approved by: portmgr (pav)
Reset inactive maintainer (recent timeouts).
Hat: portmgr
- Update from v1.09 to v1.11
- Update pkg-plist
- Add WWW and Authors to pkg-descr
PR: ports/98178
Submitted by: aaron
Approved by: maintainer timeout, tobez (implicit)
Approved by: krion@
Unbreak conditionalized Jcode.pm path.
Reported by: pointyhat via kris
SIZEify (maintainer timeout)
utilize SITE_PERL
Submitted by: Cheng-Lung Sung <firstname.lastname@example.com>
add p5-Unicode-MapUTF8 1.09
Perl class that implements conversion between arbitrary charsets
Submitted by: Ryan T. Dean <email@example.com>
Servers and bandwidth provided byNew York Internet, SuperNews, and RootBSD
No vulnerabilities have been reported in the past 14 days
* - modified, not new
All vulnerabilities


text:
Re: James Brunton, b. 1819 Scotland
In reply to:
Re: James Brunton, b. 1819 Scotland
re inventor and engineer
You may have the wrong person.
William Brunton (26 May 1777 – 5 October 1851) was a Scottish engineer and inventor.
He was the eldest son of Robert Brunton of Dalkeith, where he was born. He studied mechanics in his father's watch and clock making shop, and engineering under his grandfather, who was a colliery viewer in the neighborhood.


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Jimmy Dean® Skillets, Southwest Style: Customizable Breakfast Burritos
1 package Jimmy Dean® Skillets Southwest Style
6 eggs
8 oz. salsa
8 oz. cheese
8 oz. sour cream
16 tortillas.
Cook Jimmy Dean Skillets according to package instructions.
Spoon egg mixture into warm tortilla and top with shredded cheese, salsa and sour cream
Pairs Well With
Jake's friends spend the night and you have four hungry boys in your kitchen looking for breakfast. Customize these easy breakfast burritos for a simple solution without the hassle.
Disney's Tinker Bell Secret of the Wings CookbookBy BakeSpace Fairies
6 Recipes450 Downloads


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Number 31 *****
31 Leeson Close, Dublin 2 (Ireland) Route
Welcome to Ireland
City Centre Location
Number 31 is located in the south city centre within a 5 minute walk of St. Stephen's Green, a 10 minute walk to Grafton Street which is the main shoppping street and a 10 to 15 minute walk to the National Gallery, National Library, National Museum and the Boolk of Kells at Trinity College.
Download Map & Directions (PDF)
- Start out from Dublin Airport
- At Airport Roundabout take the 2nd exit onto the M1 (signposted Dublin). At roundabout take the 2nd exit, and then join the M1 motorway (signposted Dublin).
- Continue forward onto Swords Road - N1 (signposted M1 City Centre).
Entering Dublin at traffic signals continue forward onto Drumcondra Road Lower. Continue forward onto Dorset Street Lower - N1 (signposted City Centre)
- Turn left onto Gardiner Street. Continue forward to the last set of traffic signals. At traffic signals continue forward; follow traffic veering left onto Memorial Road. Cross the Talbot Memorial Bridge.
- Turn right onto George's Quay. Continue forward onto Burgh Quay.
Turn left at O'Connell Bridge onto D'Olier Street. Continue forward veering right to College Street and College Green.
- Continue forward veering left around Trinity College (on your left). Turn left onto Nassau Street. Turn right onto Kildare Street. Continue forward along Merrion Row.
- Turn right onto Pembroke Street Lower. Continue forward through Fitzwilliam Square and onto Pembroke Street Upper. Turn left onto Leeson Street Lower. Turn left onto Leeson Close at Starbucks.


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This Week's KidVentures {May 17}
The weather has been PERFECT for some easy KidVentures this week. We even got to break out the sprinkler for the first time! Which is always a hit for about seven minutes….anyone else experience this? I'm not sure if it's because the water is too cold, or the sprinkler isn't that exciting, or what the non-draw is! {Vent it out with me in the comment section!}
There were melted marshmallow and chocolate mouths that come from the pure pleasure of ingesting s'mores…
There was a team effort bug hunt for butterflies, dragonflies, and spiders (eeeek!),
And we even a "magic" tree house discovered in the woods.
Hello, Simple Outdoor Pleasures of Summer! Welcome back!
If you need some simple ideas for this upcoming season – KidVentures is chock-full of them! From building a homemade sprinkler from PVC pipe, to how to skip rocks, to making chalk sidewalk people, to geocaching… great ideas that you can pick up and go!
As a fellow mom of four boys, I'm always looking for ways to keep my wild bunch active. When my boys get too wiggly and wild in the house, we say, "Are you acting like an animal? Where do animals live?". They always run to the closet and grab their shoes and answer, "Outside!". Kidventures is so comprehensive and detailed, Jen makes it too easy not to head out and play. I'm looking forward to putting KidVentures to use this summer, making sweet memories with my boys while keeping my house and sanity intact.
– Hayley Morgan,TheTinyTwig.com, Founder of The Influence Network
Oh and good news! KidVentures is now available on Nook! And I've had a handful of people who are new to ebooks asking if they could get a copy without a reader or tablet. ABSOLUTELY! You can download the PDF and read it on your computer, copy it off, or read it from an app on your phone!
I was going to spend a few hours planning summer fun for my 3 kids, now I don't have to. Jen's book is PACKED with fun, simple experiences that are very well laid out and clearly explained. Honestly? I'm just as excited as my kids are about doing these activities!
-Kat Lee, InspiredToAction.com
So what KidVentures are YOU conquering first?


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From buns to bids: Bake Off inspires eBay shoppers
The Great British Bake Off is one of the most influential television programmes when it comes to viewers shopping online, eBay said.
The hit show is thought to have inspired a sharp rise in shoppers clicking on baking product listings when it returned for a seventh season, the auction site found.
The data is part of eBay's first ever UK Retail Report, released to mark the online auction house's 21st birthday.
Birmingham-based gangster drama Peaky Blinders also appeared to have an influence on shopping habits, the report suggested, with sales of flat caps tripling after the season three premiere earlier this year.
Game of Thrones and the Olympic Games were also thought to be triggers for so-called dual-screen shopping, where viewers surf the net while watching television.
EBay said it analysed user activity minute-by-minute on various devices both during live programming and in the weeks following box set releases.
Since its launch in 1995 eBay has gained 164 million active buyers and one billion listings worldwide.
Murray Lambell, director of UK trading at eBay, said the site had built itself on the theory of supply and demand.
"Over eBay's lifetime, technology and connectivity have accelerated our pace of life and opened up the world around us. We now have information at our fingertips, supercomputers in our pockets, and are part of a global community that's always open for business.
"In Britain, consumers are among the savviest in the world. They're always on the hunt for great value, quality and a price that suits them, and they are willing to shop around for the best deal.
"This simple story of supply and demand has built eBay in to an incredibly vibrant marketplace, where Brits love to shop."
Press Association


text:
Training Day Trailer (2001)
"The only thing more dangerous than the line being crossed, is the cop who will cross it." 05 October 2001 Drama, Action, Thriller 122 mins
On his first day on the job as a narcotics officer, a rookie cop works with a rogue detective who isn't what he appears.
Watch the official Training Day 2001 trailer in HD below or find more Training Day videos on Vidimovie.


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Our catering packages are currently being updated. For information on catering, please call (915) 242-5789.
When it comes to setting a truly exotic stage for your special event, no place compares to the El Paso Zoo. Enjoy the Zoo during or after-hours in an extraordinary setting unlike anywhere else in town.
Join the pride and join the fun! Whether you're planning a birthday party for a herd of kids, a reunion or wedding for your fine-feathered friends or a business luncheon or company picnic for a pack of co-workers - think outside of the banquet hall box and join us at the El Paso Zoo.
Your wild experience begins once you enter the main gate into the Zoo's expansive green space! When you combine our "one-of-a-kind" atmosphere, first-class facilities and mouthwatering menus, you've got an unbeatable combination for a celebration! The Zoo has an executive chef on staff and the catering department takes care of all the details so you can enjoy celebrating with your party. The El Paso Zoo offers an inspiring, energizing and decidedly unique venue to create a memorable event your guests will talk about for a long time to come. Best of all, by choosing to host an event at the Zoo, you're helping us secure a better world for animals worldwide!
Start planning your next event here at the El Paso Zoo. Where else can you gaze at exotic animals as you dine?
Our catering packages are currently being updated. For information on catering, please call (915) 242-5789.
Make memories with your family at El Paso's Most-Visited Family Attraction! Whether you enjoy the Zoo during the day or come to play after hours, the El Paso Zoo is tons of fun for everyone! The El Paso Zoo can help you host family reunions, picnics, private playground parties and more!
Tree House Playground Party Package - Enjoy the ultimate after-hours adventure!
Zoo-per Birthday Party Package - Enjoy the WILDEST birthday party in town, without the hassle!
Or you may Zoo It Yourself and host a party.
Wedding Packages - Coming Soon!
Group events must be planned through Taste of the Wild Catering. Groups are defined as any organized group of more than 20 people such as family reunions, corporate outings and nonprofit organizations.
Group events include a private facility and seating area. Public shaded and seating spaces are provided as a courtesy to general zoo guests and groups shall not occupy or hold these areas. Groups occupying or holding those spaces will be asked to vacate. Please call us for more information regarding renting any Zoo space or facility for your group.
Educational groups planning a field trip should make their reservation at: http://www.elpasozoo.org/education-and-tours/field-trips.


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My gpa is in a facility in Rancho Mirage. I drive 700 all at once to see him about every 30 days. I am tired when i get there and look forward to my stop at Hilton first! Sometimes I get in right away and...More
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"We had a pool view on the second floor. I recommend the third floor."
"3rd floor would be the quietest. Would stay away from elevator and the stairs"
"For three adults, the suite had two q. beds, large sofa, large desk area plus kitchenette. Would be nice..."