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|Property type
The Jewel of Cabo Pulmo is a one-of-a-kind, hand-built and hand-crafted home that features beautiful Mexican tiles and bright colors throughout. It captures the free spirit of Mexico, and the laid-back comfort of Latin America.
There are 3 ways to enjoy this beautiful property:
1) Rent the main house - sleeps up to 4
2) Rent the garden house - sleeps 2
3) Rent both - sleeps up to 6
- The main house has a queen bed in the master bedroom, and full bathroom.
- A fold-out futon in the living area accommodates 1 adult or 2 small children. A cot is also available.
- The second bedroom with a queen bed and bath are in the Garden House (or 'casita')
House amenities include:
- A comfortable, well-equipped chef's kitchen with gas range and oven, coffee maker, blender, refrigerator, and everything you need to prepare and share wonderful meals.
- A gas fired grill
- Two dining areas: one indoors, one outdoors
- A stereo system that accommodates your iPod
- Speakers indoors and on veranda
- A TV set for watching DVDs
- A library of movies and TV shows
- A satellite system for internet access (for web browsing and email, not sufficient for streaming or large files)
- The house is entirely solar powered, and has ample power for most everything - except electric hair dryers...
- Beach gear (cooler, towels, boogie boards, some snorkel equipment, and beach toys)
- Ceiling fans throughout
The second bedroom is in the Garden House.
- Located just a few steps away from the main house.
- Separate and private, with a queen bed, full bathroom and porch.
- Kitchenette with stove, refrigerator, coffee maker, everything you need for meal preparation
- Beautiful garden view.
- You may rent the Garden House as part of the main house or separately.
This is a large, private property, located in the center of the small resort village of Cabo Pulmo, just steps away from the dive shop, the beach, and all the restaurants.
The nearest beach is a calm one, suitable for children and marvelous for a dip at the beginning or the end of the day. It is only a 2-minute walk from the house. There are numerous other beaches within a short drive north or south of Cabo Pulmo.
The house is surrounded by a half-acre garden that offers privacy, beautiful views and abundant birds and wildlife. A solid, 6 foot privacy fence encloses the house and garden.
There is internet at the house suitable for email and surfing the web, a great sound system, and a library of DVD's.
The best way to check availability is by dropping us an email. We'll reply as soon as possible! We're also available by phone. We'd love a chance to chat with you about the property, Cabo Pulmo, and Baja in general.
We welcome you to our one-of-a-kind gem, hidden away in a little corner of Mexico.
Scott & Janning Kennedy
P.S. A hearty 'thank you' to Bruce Lyon, who visited with his family in 2013, for the beautiful bird photos he allowed us to use!
Keywords: House, casita, villa
My husband and I both work in agriculture. He is involved with vegetable seed production and I with organic certification. We raised our family Monterey/Salinas area of California, and currently live and work in Chile.
Janning Kennedy purchased this house in 2006
You have to see it to believe how special this place is. It is the most appealing, most comfortable place we've ever been. There's world-class surfing for my husband and a huge, beautiful, native garden for me. It's everything we could want. The house is both artful and designed for comfort. It's like living outdoors, only with all the comforts of a modern home. It's simply perfect.
We both love Mexico, especially the Baja, for its wonderful people, warm oceans and beautiful landscapes. When we crest the hill overlooking Cabo Pulmo we are looking down on our dream home. As we enter the property, we let go of pressures and stress, take a deep breath, and just relax. It is the perfect home: beautiful but unpretentious, warm, unique, and inviting. We love sitting on the porch and identifying birds and wildlife in the garden, and going up to the roof to watch the brilliant stars at night. We think you'll find it one of your favorite places on earth, and want to return again and again, just like we do.
This beautiful, half-acre property boasts a delightful garden of native & tropical plants; bird-watching is superb right from the porch. Restaurants & activity centers are 1 to 2 minutes walking distance. World-class deep sea fishing available outside the Marine Reserve, just a short drive away.
The Cabo Pulmo Marine Sanctuary is a world-class preserve that has rebounded spectacularly in the past 10 years. Diving and snorkeling are excellent, as is surfing, beach combing and hiking in the surrounding hills.
Cabo Pulmo is known for it's quiet, laid-back, relaxing lifestyle.
Beautiful,I thoroughly enjoyed my stay here at the Jewel. I had 3 people in my party, so my daughter had the little castia on the property and my husband and I had the main house and, my friends were next door at el encanto. it was perfect for a big group, everyone had there space within walking distance and the locals in town were very nice. It was so nice to cook and eat at home and enjoy the beautiful veranda. we also went out twice and had some very nice meals within walking distance. The only time we drove was to go snorking at the near by beaches.There was so much bird life and I felt safe having it gated in for are cars and are dog. Karl was very nice man and very helpful when we arrived. There was plenty of towels and snorkeling gear boogie boards chairs etc, which was nice. I hope to come back next year for sure.
The only thing I have to say is the stove needs some work, its not bad it just takes forever to heat stuff, its probably clogged a little. And for anyone that needs a bathroom in the middle of the night its not in the bedroom, its by the kitchen, which did not madder to me because its all gated in and very private.
I'm still here , sitting on the verandah and watching the birds while I drink my morning coffee.
It couldn't be more perfect! Thank you
What a delightful find and a marvellous introduction to Cabo Pulmo National Park. We rented the whole enchilada and well worth it in every way.
Based in La Paz with a three day break in Cabo Pulmo.
Arranged booking from UK & Janning the owner so prompt and accommodating with our request.
Loved the Santana music as backdrop to
U Tube property advert!
The drive down from La Paz was good and no problem with dirt roaIt as you hit C.P. Wonderful wildlife en route.
Spectacular scenery all around the property and so private and peaceful.
Karl had an airport run so he left everything just perfect for us and we met up later.
The Mexican artistry in main casa is amazing with interesting books to read from art to surfing. Looking at build plans one can see it has been a labour of love by the owners.
Off grid: big fridge and all mod cons. Just bring food and drink- although La Cabellera Cantina opposite has extra large water containers for sale - useful as weather absolutely roasting.
It was the only one of two open at the time as very few tourists. Food was delicious and plentiful with friendly staff.
My casita was well equally stocked and did not want for anything.Bed was most comfy! I kept all the windows open at night as insect screens in place. Felt very safe - no need for locks here.
My daughter & partner soon got used to their new bedfellows: giant owl butterflies and a bat that demised on the outside wall!
We spent hours watching and photographing the birds with Karl topping up the Hummingbird water bottles and bringing bird seed. Red Cardinals/ / Mexican Jays / Cactus Wrens/ varieties of Hummingbirds / Greater Roadrunners / Crested Caracaras / Hooded Orioles / Flickers and woodpeckers were amazing. The Hummingbirds have big personalities and were remarkably used to humans. Those tiny feet!!
The beach is near but steep with huge waves at the time. I'd researched best snorkelling spots and Los Arbolitos about 15 mins away was truly wonderful. Swam right out and amazing coral and fish variety. Safe and easy access into sea. Busy at weekends with local families - quieter in the week. Even when busy it's light years from English beaches re: amount of people!
Beach towels : parasol & chairs provided with cooler.
The roof top main casa offers panoramic view of the hills behind and sea in front: fantastic sunrises and sunsets. Minimal mozzies as too hot.
If you want a taste of paradise even for a few nights you won't regret booking up!
We spent a week at the beautiful "Jewel"! After taking nine days to drive down the Baja, camping along the way, it was like heaven to arrive in Cabo Pulmo and stay put for a while! Everything about the house, village, and area were as good or better than we expected! It really is a magical place. While at the house we were either on the roof or playing cards on the porch. It was also fun to make wonderful, fresh meals in the kitchen. Everything is just beautiful from the garden, to the tile, to the decor and furniture. The activities in Cabo Pulmo were the things we like..... beach walking, swimming, scuba, snorkeling, and hiking. Sunrises and sunsets were spectacular. We can't wait to return!
My husband and 8 year old son stayed at the villa for a week and cannot say enough good things about this stunning place. The entire experience from booking to checking out was excellent. The Jannings responded to our request immediately, and our communications with Karl, the caretaker were very helpful. Karl is a wonderful person and checked in on us regularly to make sure we had everything we needed. The home is really beautiful, a perfect place to take breaks from the beautiful beaches and snorkeling. Well furnished kitchen, comfortable beds, beautiful deck to lounge on. It has a beautiful and large garden that is full of local birds and creatures. The town of Pulmo is remote and is about 90 minutes from Los Cabos Airport, the last 30 minutes of which you are on potholed and dirt roads. The roads are totally manageable, you just need to take your time and just enjoy the ride. Totally worth the trek out there, though, and we're already planning our return trip for next year.
My wife and I recently had a 3 night stay in the villa and truly loved the experience. The owners were prompt with e-mail responces and even a phone call during the booking process. Booking was simple and clean and Jannings information package supplied with the contract was great. Directions were exact and very helpful. Quite the experience living for a few days of the grid but once we settled in it really was not an isssue. The villa was equipped with everything you need. We would highly recomend this villa for anyone who wants a unique experience in the Cabo Pulmo area
|Pet Fee
VRBO quote applies to 1 bedroom only! For rates for 2 bedrooms (main house and Garden Casita), contact owner.
$200.00 security deposit *authorization* on credit card required. Not deducted unless there is a problem.
Cleaning fee $50 applies to stays of 1 or 2 nights.
Futon or cot set-up fee $15.00 each.
10% discount for stays of 10 nights or more.
Contact owner to confirm VRBO price quotation!
For reservations > 30 days in advance, 50% due at booking, 50% due 30 days before arrival.
For reservations < 30 days in advance, total rent is due at booking.
Major credit cards (VISA, MC, Discover) and PayPal accepted.
Rates are subject to change until reservation is confirmed.


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NEW YORK DOLLS - One Day It Will Please Us To Remember Even This (CD)
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The legacy and influence of The New York Dolls is arguably greater than the immediate recollection of one of their revered songs, and after 30 years the band are back with a new studio album, Hailing from the early 70's glam rock scene, the irreverent Dolls mixed the glam of Bowie, the swagger of the Stones, and the raw power of the Stooges to create a gateway which saw the rise of legends such as The Ramones, KISS and eventually the Sex Pistols, which became Malcolm McLaren's next project after his 'failure' with the Dolls, Having been invited to reform for the Meltdown Festival by former fanclub President Morrissey in 2004, the band signed to Roadrunner in 2005 and now deliver a brand new studio album sure to pique curiosity in those who recall and acknowledge their well deserved legend.
Cat No: RRD6180452
Barcode: 16861804527
Packaging: CD Regular
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subset: commoncrawl


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Lesbain Vegans
I had a dream last night that being a lesbian vegan for too long would turn you into a vampire, and all these lesbian vegans were growing wings and teeth. I have nothing against lesbians or vegans, it was just my subconscious creating some sort of surrealist plot. What do you think it means?


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MOVIE SYNOPSIS [ General Audiences ] Being a production of USA, this feature film of 1952 has been excellently directed by George Sherman. You can watch Back at the Front online on TV cable or theaters with original audio in English at different dates. This film premiered in theaters in 1952.
Rating: 5 by 37 users./
Runtime: 87 minutes
Tom Ewell, Harvey Lembeck, Mari Blanchard, Richard Long, Gregg Palmer, Barry Kelley, Russell Johnson, Vaughn Taylor, Aram Katcher, Aen-Ling Chow, Benson Fong, Mal Alberts, Howard Banks, Ray Barnes, Lane Bradford and Robert Bray.
87-minute film (full movie) directed by George Sherman.
The original audio of Back at the Front has dialogues in Dolby Digital, it was produced in USA.
It premiered in cinemas or television in 1952.
Screenplay written by Bill Mauldin, Lou Breslow, Don McGuire and Oscar Brodney.
Clifford Stine.
Pat Betz and Leonard Goldstein.
This film was produced and / or financed by Universal International Pictures (UI).
Composed by Herman Stein and Henry Mancini.
Universal Pictures.
Without commercial breaks, the full movie Back at the Front has a duration of 87 minutes; the official trailer can be watched in streaming on the Internet. You can watch this full movie free in EnglishDolby Digital in movie TV channels, renting the DVD or with VoD services (Video On Demand player, Hulu) and PPV (Pay Per View, Netflix).
See the page of this full movie, Back at the Front in FULLTV Movies.


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How's it been for you? Have the words been hotter than Fahrenheit 451, or has The Beast from 20,000 Fathoms gotten in the way? Here's your chance to tell everybody about it, whether you're a weekly or monthly (or anything in between) W1S1 participant.
If you've managed to write a story and submit a story, shout it from the rooftops. Yahoo! If you've had something W1S1-related accepted, tell us so we can add you to the Write1Sub1 Hall of Fame. But if stuff has interfered with your writing and every reply has been a rejection, let us know that too so we can commiserate. We're all in this together.
Also, remember to note your paid publications ($) for our Published Work$ page -- stories published from W1S1 2011 - 2013, and don't forget to post the link. While W1S1 continues to encourage all avenues for publication, we want to especially highlight those stories and markets that help to put protein-rich snacks in a writer's fridge.
Successes and failures, triumphs and disasters, brilliant ideas and insane dreams: we want to read all about them.


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Extended Black Friday Sale Event
Sold Out
Bulova gentlemen's line of time pieces blends durability with fashionable appointment and clever detailing. Designs sport traditional solid face or recently popular exposed mechanism styling. NZ Watch Store offers wide range on Bulova watches at an affordable price.
The sophisticated yet stylish design of this Precisionist watch by Bulova allows this watch to be worn in any professional setting. This versatile timepiece has a silver stainless-steel band that allows for a comfortable fit and a durable mineral crystal that helps protect the watch from excessive damage. This watch having 300 meters / 984 feet / 30 atm water resistance it makes it even more useful. The durability and functionality of this timepiece makes it an ideal watch for the workplace or any professional setting.
Analog Display Grey Dial Gold Hands Gold Applied Indexes Movement
Quartz Date Display Calendar 300 Meters / 984 Feet / 30 ATM Water-resistant Case
Round Shaped Stainless Steel Men's Size 45mm Wide (without crown) 18mm Thick Band
Silver Stainless-Steel Band Fold Over Clasp with Safety 22mm Wide Mens Standard Length Features
Luminous Water-resistant Chronograph Screw Down Crown Measures Seconds
NZ Watch Store offers two shipping methods:
|Shipping Method
|Shipping Cost
|Processing & Delivery Time
|4 - 7 Business Days
|8 - 9 Business Days
All orders are dispatched through NZ Couriers and require signature on Delivery. Tracking information will be sent via email with the tracking link. Customers will be updated every time status of the order is updated.
All Our Products are brand new, authentic, in the original box with manuals.
Your comfort, security and confidence are our utmost concern. All transactions are digitally encrypted using the latest technologies. We never share any of your information. All payments are processed by Paypal and our store's security is certified by McAfee.


text: Hi guys, I have been using the Prince Exo3 Rebel 98 since 2010. I am just 3.5 player. This is my only racquet in the bag, and was looking for a change, a new racquet. I seem to have no power with this one, i have good form, and swing, yet, it doesn't come anywhere close to the baseline. So, looking for a good recommendation on racquet that will give me more power, and less arm hurting. Thanks guys.


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Available for Picture Editor bookings and Photography commissions. Simply get in touch.
Tuesday, 2 April 2013
West Ham UTD v West Bromwich FC.
Went along to see the West Ham UTD on Saturday with the added luxury of spending it in the hospitality of the 66 club. We where given a we look around the ground shortly before being entertained by West Ham legends Martin Peters and Phil Parkes. I managed to get a glimpse of Sir Trevor Brooking too.
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Harnessing the power of water represents one of the oldest forms of energy. Renewable, and comparatively non-polluting, it is a significant factor in meeting green energy targets.
DECC suggested that in 2013 approximately 1.7% of the UK's total generating capacity and approximately 22% of renewable generating capacity in Scotland was hydroelectric.
Small scale hydroelectric projects provide significant opportunities to landowners with suitable sites, providing long term, predictable energy generation with minimal impact on other land uses.
Large-scale hydroelectric power stations are mainly the preserve of leading electricity companies and require significant due diligence exercises to ensure their delivery in almost any setting.
Despite their renewable nature, large hydro schemes can have a significant impact on their setting and therefore the strategy, site selection viability appraisals and the subsequent permitting and instigation of projects must be dealt with appropriately.
Storage of electricity is becoming an increasingly important subject as more of UK's capacity originates from intermittent forms of renewable generation.
Pumped storage hydro is a well proven and reliable form of storage for large amounts of energy, supporting intermittent forms of generation in particular.
Savills is advising a number of landowners about the hydro potential of their property and can guide clients through the entire process as follows:
Nick Green
Energy & Infrastructure
Savills Perth
+44 (0) 1738 477 518
+44 (0) 1738 477 518
Alex Smethers
Rural Surveyor
Savills Margaret Street
+44 (0) 1223 347 275
+44 (0) 1223 347 275


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Curriculum & Content Solutions : English Language Acquisition
There are 15 solutions in this sub-category. Use the filters on the left to refine the list.
Browse the Menu | Search the Menu | Evaluation Process | Student Data Privacy | Usage Reporting | Contact | Admin
©2016 Denver Public Schools


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- select colour
it's an internet thing!
it's an internet thing!
09:44 08 November 2011
Strictly Come Dancing has been plunged into crisis, as it emerged that one of the shows stars has fractured his spine.
Artem Chigvinstev, who is the professional partner of Holly Valance, has been diagnosed with a transverse process fracture of the lumbar spine.
The injury, which was initially diagnosed as a strained muscle, was sustained during rehearsals for last weekends show.
Despite the immense pain caused, he and partner, former Neighbours star Valance, danced their way to a score of 34 out of 40.
A BBC source told the Daily Mail:
"At the moment, Artem is not dancing but he is attending rehearsals and choreographing the new routine. He wants to dance, but his health is our priority. We have a duty of care to him."
Another insider added:
"Heaven knows how Artems going to dance on Saturday. This is a serious injury."
With Chigvinstev undergoing physiotherapy, the Australian actress is now training with the recently eliminated dancer Brendan Cole, who could perform the rumba with her this weekend.
In a statement issued last night, the BBC said:
"Artem injured himself during rehearsals on Friday. Following medical attention he has been diagnosed with a transverse process fracture of the lumbar spine.
"We have been told that this sort of fracture is completely stable and he has been advised that there is no untoward effect from him continuing to dance. We will, of course, continue to review this following medical advice over the course of the week."
Disclaimer: Supanet is not responsible for, and disclaims any and all liability for the content of comments written by contributors to this website


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One in four drivers do not know what type of car insurance they have, research by the Chartered Institute of Insurers (CII) has revealed.
The CII, which polled 2,000 UK motorists, also found that more than a third of drivers believed that comprehensive cover would pay for the damage if they had an accident driving another car.
Comprehensive car insurance usually offers third party coverage for motorists using other vehicles, only paying for the damage to other drivers and their cars.
Meanwhile, 35% thought that if a sneeze caused a crash their insurance wouldn't cover it, 29% believed UK car insurance is not valid in the EU and one in ten thought an excess is a type of premium insurance policy.
However, sneezing behind the wheel will not invalidate a claim, UK car insurance includes the statutory required cover to drive in the EU and an excess, far from being high-quality cover, is the amount the policyholder has to pay in the event of a claim.
Find out more: Which Recommended Providers – best and worst car insurance
Buying car insurance: Finding the right cover at the right price
Car insurance may be a confusing grudge purchase but it doesn't need to leave you out of pocket. Follow our tips to cut the cost of cover.
Set the right excess
If you're willing to pay more for your excess your insurer will lower your premiums. However, make sure you select your excess carefully. Setting the bar too high, especially if it's too close to your claims limit, might make claiming on it pointless and expensive.
Choose your occupation carefully
The job categories used by insurers to help set your premiums can be broad, meaning that many drivers could fit into many different segments. For example, if you're unemployed but are a housewife or househusband, putting yourself down as a homemaker instead can help you save money.
However, remember that you should never lie about your job. Don't say you are a butcher if you are a baker. This is considered fraud and you could be prosecuted.
Pay annually (if you can afford to)
Paying for cover on a monthly basis involves taking out a loan, usually at high interest, from your provider. The APR can vary but it can be as high as 40%. Some insurers will also charge you a fee for setting up a monthly payment plan.
- Claims satisfaction – how the big car insurance brands rate on claims handling
- Car insurance reviews – detailed policy information for all the major car insurance providers
- How to claim on your car insurance – how to submit and follow through on a car insurance claim


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14K White Gold Jewelry 25.0 ctw Tanzanite & Diamond Necklace - SKU#U... Lot 3907
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- Sold Date:
- Source: eBay
Gemologically tested Jewelry 14K White Gold Gemstone Necklace ---- Manufacturer's Suggested Retail Price - $20880.00 ---- Diamond: 72 Round cut @ 1.0 ctw ---- Minimum Diamond Clarity: SI3-I1 ---- Minimum Diamond Color: I-J ---- Gemstone: Natural Tanzanite ---- Gemstone color: Purple ---- Gemstone quality: Good-to-Very Good ---- Gemstone Cut & Weight: 25 Oval cut @ 24.0 ctw ---- Metal Purity & Color : 14K Gold-White ---- Gold weight: 30.1 grams ---- Dimension: (inches) Approx. 16 1/4 x 1/4 ---- Shipping: Ships within 10 business days or faster ---- SKU#U268X1- 1140-14K ---- US Jewelry Liquidation specializes in Bridal, Engagement, Certified Diamonds, Gold Coin, Silver Coin, Watches, Antique Collectibles, Memorabilia and Vintage Estate Auctions.Proudly Made in USA Condition Report: NEW Payment: The buyer is responsible for paying the seller directly after winning the item. Details on accepted payment methods and where to send payment are provided in an invoice from the seller. Shipping: The buyer is responsible for paying all shipping costs and arranging for shipping and delivery with the seller. Additional shipping details from the seller: -We offer FREE USA Shipping for United States orders. USA shipping includes Tracking and Insurance. ... read more


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Chelsea, MA Kayaking
Explore Chelsea Kayaking
Find Chelsea Kayaking players, teams, games, leagues, and activity partners. Recruit for your teams, promote your leagues, or just see what's happening in Chelsea Kayaking... and it's all safe, secure, and easy.
Connect around Chelsea Kayaking
Post bulletins and create your Kayaking profile to connect with other Chelsea Kayaking players and teams. Use the Bulletin Boards to help you meet new friends, recruit for your team, promote your league, and get your game on!
Organize Chelsea Kayaking Teams, Leagues, & Events
Manage your Chelsea Kayaking teams online. Sportsvite makes it a cinch, with team email, rosters, RSVPs and more! Start pickup games, manage team schedules, set invite alerts and reminders... even vote for MVPs!
Chelsea Kayaking Partners Find More Kayaking Players Create a Profile
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Chelsea Kayaking Bulletins Find More Bulletins Post a Bulletin
In the mood for Kayaking? not in the past month by Michie
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Chelsea, MA Kayaking on Sportsvite
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Schedule your tank to be pumped by us for a low price and excellent service:
- Schedule your septic tank pumping service online by completing this form or by calling (251) 971-3022
- We currently only pump septic tanks on weekdays
- We do not install, repair or replace septic systems
- BCSS does not provide a formal septic system inspection while pumping the septic tank. The Baldwin County Health Department is an option for a formal inspection, and they can be scheduled by calling (251) 947-3618. Our septic tank pumping crew can arrange to meet inspectors on site.
- If our sanitary sewer service is available for your property, you may be eligible to connect at a discounted rate with our Environmental Enhancement Program. Contact us at (251) 971-1516 to see if we can provide reliable sewer service to your property.
Septic Tank Pumping Pricing
- $275* for one tank
- $200* for each additional tank on the same lot
- *Small franchise fee may apply if in a franchise area
Our Services
- Find out how often your tank should be pumped here.
- Environmental Protection Agency Septic System Tips
- Additional Resources
- Schedule Septic Tank Pumping Today


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One hundred years ago in 1912, a young architect was assigned from Hong Kong to Shanghai to expand business for his agency. With talent and luck, he got a project, executed it superbly and thus won a galaxy of notable projects, most of which are still glistening on the crescent Bund.
The architect was George Leopold Wilson, then 32, from Messrs Palmer & Turner Co. And the first project that brought him good fortune was the Union Building, widely known today as Three on the Bund.
In 1916 the Far Eastern Review newspaper called the six-story building one of the most noteworthy advances in construction and design in Shanghai - it used a steel skeleton, a technological advance that would make skyscrapers possible.
"The lines of the building are beautiful and the structure is graceful in the extreme, forming a noteworthy monument to the skill of the architects, Messrs Palmer and Turner," it said.
Even the blueprint showed a distinctive structure, notes Shanghai Tongji University Professor Qian Zonghao.
"With a corner tower topped by golden argosy weather vane 150 feet (45.72m) above the ground, the edifice is built with skeletal steel framework, a bold new technique which was used in Shanghai for the first time," Qian says.
He defined the style as "free Renaissance," marked by vertical lines expressing the skeleton construction.
According to Lou Chenghao's new book "Shanghai's Architects and Builders 1840-1949," the edifice 46 meters high was the tallest building in the city of then. The previous record was held by the 40-meter-high, Song Dynasty (960-1279) Longhua Pagoda for more than 1,000 years. Before the Union Building, the tallest structure on the Bund was the former Palace Hotel.
Three on the Bund was the first Bund building to be renovated for commercial use - a hub of high-end restaurants, luxury shops, spa and art gallery. Like the Xintiandi project, the renovation that began in 2001 was controversial because it dramatically altered the original interior. Tourists and merchants loved it while preservationists disliked it and believed it opened a kind of Pandora's box bringing "mercantilism" back to the Bund.
In the book "Images and Legends of the Shanghai Bund" (2008), local writer Chen Danyan recalled the only party held on the empty plaza right before renovation began.
"The empty building was full of the smells of cold concrete and lime powder, which mingled with the aroma of fresh basil and melting cheese from a Hilton hotel buffet serving here, and a variety of fragrance from the bodies of guests," she wrote.
"In the mixture of smells, the Bund was awake. Her thirst for money and gold, spirit of adventure and ambition to seize and develop in a heartless way, all woke up."
Dating back to the 1910s, the ground, first, second, third and fourth floors were leased out as offices, part of the first floor being occupied by the Union Insurance Society, the owners of the building.
On the fifth floor were residential flats of the highest class, with roof gardens on the flat concrete roof. These flats command a magnificent view of the river and of Shanghai. The height ensured coolness and quietness, a treasure to enjoy in a city with hot summers and without air-conditioners.
With windows on three sides, the office on the ground floor, which had been taken by an American import and export house, was the largest single office without dividing walls in Shanghai.
The building is also known for one of its former tenants, the Mercantile Bank of India, whose Chinese name You Li was used as the building's name.
In 1941 Japanese invaders occupied the building. The bank restarted business four years later but retreated from China in 1949. In 1953 another legendary architect moved to work in the building, Chen Zhi, director of the Civil Design Institute of Shanghai. A graduate of the University of Pennsylvania from the United States, the man was among the first to record and preserve the city's historical buildings after the 1950s.
"The plinth of the building is of granite, the remainder of the filling walls being of brickwork finished with an artificial stone facing," Professor Qian says. "The principal entrance was flanked by massive granite columns with carved pediments, leading to an imposing entrance hall. It is really the first masterpiece of Palmer & Turner in Shanghai."
Founded by William Salway in 1868 in Hong Kong, Palmer & Turner had a Chinese name "Ba Ma Dan Na," a Cantonese translation of its English name. As soon as the Union Building was completed, the firm moved its Shanghai office in, created a nice Mandarin name "Kung-woo," or "Fair and Harmonious" and developed dramatically in this "Paris on the Wangpoo (Huangpu) River."
Within decades the company created more than 30 excellent structures in Shanghai, including eight of the 23 waterfront Bund buildings, such as the HSBC Building and the Sassoon House. After 60 years' absence from the city since World War II, the company made its way back to Shanghai, having designed new skyscrapers since the 1990s including the Raffles City Shanghai and One Park Avenue.
"When we came here, we introduced ourselves as ?Kung-woo' and people here all were familiar with this name," says architect Lee Kian Siew from Palmer & Turner Consultants (Shanghai) Ltd, who, like Wilson, was sent from Hong Kong to expand business in Shanghai in the early 1990s.
"We have seen the opportunity and the Shanghai office has grown from 20 to 200 employees today," Lee says.
According to Wu Jiang's book "A History of Shanghai Architecture 1840-1949" (2008), Palmer & Turner grew to be the largest and most important architecture design firm in Shanghai of the 1920s and 1930s. Their works were almost a mini collection of the Shanghai architectural scene. And it seems that the history-rich firm had picked the right time to open a Shanghai office twice within 100 years.
NO. 3 on the bund
Yesterday: The Union Building
Present: Three on the Bund
Address: 3 Zhongshan Road E1, by Guangdong Road
Built: 1916
Architectural style: Free Renaissance
Designer: Palmer & Turner
Admission: Visitors can see each commercial floor. I suggest going onto the balcony for a drink where one can look up to see the stunning corner tower and overlook a perfect skyline dotted with three famous roofs of the Bund - the former HSBC Building, the Customs House and the Sassoon House, all of which were designed by Palmer & Turner.
From ¥280
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MOVIE SYNOPSIS [ General Audiences ] Particle Fever follows the story of six brilliant scientists trying to unravel the mysteries of the universe, documenting the successes and failures in the most significant and inspiring the world scientific progress. You can watch Particle Fever online on TV cable or theaters with original audio in English at different dates. This film premiered in theaters in 2013.
Rating: 5 by 2237 users./
Runtime: 99 minutes
Martin Aleksa, Nima Arkani-Hamed, Savas Dimopoulos, Monica Dunford, Fabiola Gianotti, David Kaplan, Mike Lamont, Asmina Arvanitaki, James Beacham, Jed Biesiada, Christophe Clement, Andy Cohen, Katherine Copic, Kyle Cranmer, Albert de Roeck and Georgi Dvali.
99-minute film (full movie) directed by Mark Levinson.
The original audio of Particle Fever has dialogues in Dolby Digital, it was produced in USA.
It premiered in cinemas or television in 2013.
Wolfgang Held and Claudia Raschke.
Brian David Cange, David Kaplan, Andrea Miller, Carla Solomon, Gerry Ohrstrom, Thomas Campbell Jackson, Mark Levinson and Wendy Sax.
This film was produced and / or financed by Anthos Media and Particle Fever.
Composed by Robert Miller.
Madman Entertainment, Netflix, Abramorama, Jupiter Communications and BOND360.
Without commercial breaks, the full movie Particle Fever has a duration of 99 minutes; the official trailer can be watched in streaming on the Internet. You can watch this full movie free in EnglishDolby Digital in movie TV channels, renting the DVD or with VoD services (Video On Demand player, Hulu) and PPV (Pay Per View, Netflix).
See the page of this full movie, Particle Fever in FULLTV Movies.


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Ptak, Noel & Associates LLC
+1 603 672 9514
At Ptak, Noel & Associates we help IT organizations become "solution initiators" in using IT management technology to resolve business problems. We do this by translating vendor strategy & deliverables into a business context that is communicable and actionable by the IT manager, and by helping our clients understand how other IT organizations are effectively implementing solutions with their business counterparts.
Ptak/Noel tracks the IT management market as it evolves from individual silos of systems, network, application and database management tools into a comprehensive solution set that delivers, supports and protects business transactions, services and processes. As a boutique IT analyst firm we're able to strategically focus on these requirements and benefits of Business Service Management (BSM) for both our vendor and consumer clients.
Publications from Ptak, Noel & Associates LLC
Case study


text: ST. LOUIS Marty Havlat scored in overtime, his second of the night, as the Sharks beat the Blues in double-overtime on Thursday night at Scottrade Center, 3-2.After a long shift by the Sharks second line early in the second extra frame, Havlat fired in a slap shot from the high slot at 3:34 on a feed from Ryane Clowe.The Sharks lead the best-of-seven series, one game to none. Game 2 is here on Saturday.It took just 54 seconds into the third period for St. Louis to erase a 1-0 deficit, when Patrik Berglund tipped a floating wrist shot by Kevin Shattenkirk past Antti Niemi.After Havlat tripped Jaroslav Halak behind the St. Louis net, Berglund deposited his second of the night with a wrist shot from the slot at 7:28 on the power play. Berglund trailed a rush up ice after the Sharks got caught too deep on a shorthanded rush of their own.San Jose responded. Tommy Wingels drove around Roman Polak and backhanded the puck to a charging Andrew Desjardins, who slipped it through Halak at 14:44.Niemi kept the Sharks in the game in the first overtime, as the Blues dominated territorially for virtually the entire 20 minutes. His best save came with just 35 seconds to go, when he got just enough of David Perrons laser from the circle.San Jose nearly won the game moments earlier, when TJ Galiardi spotted Torrey Mitchell in front of the net. Halak inadvertently kicked it out to the slot and was looking the wrong direction, but the puck was cleared out just in time before Galiardi could find it, as he charged in from the corner.After a scoreless first, Brent Burns drew a retaliatory slashing penalty on David Perron, putting the Sharks on the power play in the second period.They capitalized at 6:02. Dan Boyle had all day to skate with the puck towards the high slot, and his wrist shot deflected off of Havlats stick and over the shoulder of Halak.It was San Joses first goal at the Scottrade Center in 2011-12, after they were shut out in the two games here in the regular season. The power play had been a combined 0-for-10 in those two losses.San Joses penalty kill was tested twice in the frame, and passed both to keep that lead at the intermission barely.The Sharks impressively killed off a Jason Demers interference call midway through, but things got dicey with TJ Galiardi in the box for interference at 16:54.Niemi made another fantastic save on Alex Pietrangelo at the side of the net, swinging his left pad out to deny the Blues defenseman on the doorstep. Later, Berglund had a look at the net alone in the slot on a rush, but fired high.Alex Steen hit the post on a shorthanded rush with four minutes to go in the second.Odds and ends: Colin White, Jim Vandermeer, Michal Handzus, Brad Winchester and Benn Ferriero were the Sharks scratches. The Sharks started the first round on the road for the first time since 2007 in Nashville. Andrew Desjardins has two goals in four career playoff games. Tommy Wingels recorded an assist in his first career NHL playoff game.


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A Ben Sims setlist is a compilation of all the songs Ben Sims has played live in concert. Many Ben Sims concerts have the same setlist, and sometimes Ben Sims setlists will vary from night to night. We've gathered together 2017 Ben Sims tour setlists as well as past Ben Sims setlists to give you a better idea of what you can expect at your local Ben Sims show. If you have a Ben Sims song list you would like to upload, please check back as that functionality will be added soon, as well as setlists for Ben Sims.


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Watch Lethal Weapon 4 online free spacemov, Martin Riggs and Roger Murtaugh, after escaping death from the previous movies are put on a hit list by The Triads. When blood thirsty mercenaries are on their tail they team up again with Leo Getz and Lorna Cole, a newcomer (Chris Rock) to finally put an end to the Triads for good.
Genre › Action, Crime, Thriller
Release › 8 May / 1998
IMDB Ratings › 6.6
Duration › 2h 07 min
Director by › Richard Donner
Written by › Shane Black
Country › USA
Language › English
Production › Warner Bros.
Starcast › Jack Kehler, Mel Gibson, Danny Glover, Joe Pesci ,Darlene Love


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By Rep. Penny Bernard Schaber
Between the time I write this column and the time you read it, one of the most important milestones in the long saga of the Affordable Care Act (ACA) will have passed. On January 1, insurance plans purchased from the health care exchanges will begin their coverage. For many Americans who have been locked out of health insurance for so long because of exploding costs or preexisting conditions, this will be the first time they've had health insurance coverage. For others, it will be the first time they've had insurance they could actually count on to cover them when they get sick. While anyone who's followed the news over the last few months—or indeed, the last few years—could tell you that the ACA's path has not been smooth, I think many of the pitfalls along the way were avoidable.
Last month, in response to news reports of problems with the federal health care enrollment website, Gov. Walker called a special session of the Legislature to extend the timeline he set in his recent budget for kicking Wisconsinites off BadgerCare. While it is laudable that the governor sees the need to expand access to affordable health care, if only temporarily, the fact is that people will still be losing their health insurance. Governor Walker and the Republicans in the Legislature had the option to make sure nobody would lose their BadgerCare coverage, at no additional cost to taxpayers for at least the next three years, but he chose to play politics with their health instead.
During the budget debate earlier this year, residents across the state asked Governor Walker to accept federal dollars to strengthen BadgerCare and implement a state-run exchange. This funding—from a pot of money that Wisconsin families already paid into with their federal tax dollars—was a win-win for Wisconsin's working families and taxpayers and would have insured 100,000 more Wisconsinites. Strengthening BadgerCare was also poised to save Wisconsin taxpayers $120 million, funding that undoubtedly would have been welcome in efforts to provide worker training or small business grants to create jobs. In fact, states like Kentucky, Washington, and Minnesota that took this deal in the first place were able to build their own health care exchange websites and avoid the problems in the federal website that prompted Gov. Walker to propose changing the BadgerCare deadline.
Now, the governor has proposed to spend even more Wisconsin tax dollars to cover the cost of his health care plan. Under the governor's plan, almost 80,000 Wisconsinites will be kicked off BadgerCare on March 31, 2014, rather than December 31, 2014. Governor Walker is refusing to right his wrong of rejecting funds to strengthen BadgerCare. He is spending Wisconsin's taxpayer dollars to fill in part of the gap rather than laying a new foundation of expanded affordable health care with federal funding.
Wisconsin can do better. The Affordable Care Act was designed to give states flexibility to make our own decisions, and states as diverse as Arkansas, Iowa, and Vermont have taken advantage of that flexibility to offer innovative solutions. At a time when Wisconsin is lagging behind other states in job creation, economic development, and wage growth, we can't afford to fall behind in health care too.
I encourage you to contact the governor and your state representative and senator to let them know how important access to affordable healthcare is to all Wisconsinites. The governor can accept the federal tax dollars we have already paid to make sure that BadgerCare covers all of the people who need it. You can call the legislative hotline and leave a message: the number is 800-362-9472.


text: $93.4M Winner Comes Forward! A master sergeant assigned to the 58th Training Squadron at Kirtland Air Force Base in Albuquerque, New Mexico has claimed a $93.4 million Powerball jackpot. John San Cartier correctly matched all six numbers drawn on August 10. San chose the $52.2 million lump-sum option. After federal withholding taxes of 25 percent and state withholding taxes of six percent, he will receive a one-time wire transfer of approximately $36 million.


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Although production of reactive oxygen species (ROS) such as superoxide (O2·−) has been implicated in chronic hypoxia-induced pulmonary hypertension (PH) and pulmonary vascular remodeling, the transcription factors and gene targets through which ROS exert their effects have not been completely identified. We used mice overexpressing the extracellular antioxidant enzyme extracellular superoxide dismutase (EC-SOD TG) to test the hypothesis that O2·− generated in the extracellular compartment under hypoxic conditions contributes to PH through the induction of the transcription factor, early growth response-1 (Egr-1), and its downstream gene target, tissue factor (TF). We found that chronic hypoxia decreased lung EC-SOD activity and protein expression in wild-type mice, but that EC-SOD activity remained five to seven times higher in EC-SOD TG mice under hypoxic conditions. EC-SOD overexpression attenuated chronic hypoxic PH, and vascular remodeling, measured by right ventricular systolic pressures, proliferation of cells in the vessel wall, muscularization of small pulmonary vessels, and collagen deposition. EC-SOD overexpression also prevented the early hypoxia-dependent upregulation of the redox-sensitive transcription factor Egr-1 and the procoagulant protein TF. These data provide the first evidence that EC-SOD activity is disrupted in chronic hypoxia, and increased EC-SOD activity can attenuate chronic hypoxic PH by limiting the hypoxic upregulation of redox-sensitive genes.
- extracellular superoxide dismutase
- redox-sensitive transcription factor
- early growth response-1
- tissue factor
- pulmonary hypertension
adult and pediatric patients with lung diseases complicated by alveolar hypoxia are at risk for developing pulmonary hypertension (PH), a process that significantly increases morbidity and mortality (43). Production of reactive oxygen species (ROS) such as superoxide (O2·−) has been implicated in the pathogenesis of PH, including chronic hypoxia-induced PH (7, 17, 26, 28, 35, 41). A subset of these studies implicate a role for O2·− generated specifically in the extracellular compartment (28, 34). Extracellular superoxide dismutase (EC-SOD) is the only extracellular enzymatic scavenger of O2·− and is highly localized to the lung and vascular tissue (30, 36, 37). EC-SOD activity decreases in other models associated with oxidative stress, including hyperoxia and bleomycin-induced lung injury (9, 13, 29, 38, 46), although the impact of hypoxia on EC-SOD expression and activity in the lung is unknown.
Interestingly, Kamezaki et al. (23) recently reported that gene transfer of the antioxidant enzyme EC-SOD protected rats against monocrotaline-induced PH, suggesting that extracellular O2·− is important in the disease process. Mice overexpressing EC-SOD have been utilized in models of vascular and lung diseases to confirm a role for extracellular O2·− in the pathogenesis of a number of disease models associated with fibrosis and tissue remodeling (2, 5, 8, 10, 39). The effects of EC-SOD overexpression on chronic hypoxic PH and pulmonary vascular remodeling have not been previously studied. One important function of ROS is their contribution as signaling molecules to regulate the expression of redox-sensitive genes (14, 27). EC-SOD levels, and thus extracellular O2·−, modulate the classic hypoxia-inducible transcription factor, HIF-1α, as well as its downstream target, erythropoietin, in the hypoxic kidney (44, 53). The contribution of extracellular O2·− and EC-SOD in the regulation of transcription factors and downstream genes in the pulmonary circulation has not been examined. Among many redox-regulated transcription factors, early growth response-1 (Egr-1) is of interest because we and others have shown it increases in the lung and pulmonary vascular cells early in response to hypoxia, and Egr-1 is known to activate a number of downstream targets critical to the vascular remodeling process (3, 12, 22, 24, 49–51). One important Egr-1-regulated downstream gene is the procoagulant molecule, tissue factor (TF), which has been implicated in vascular remodeling in response to a variety of stimuli including hypoxia in both the systemic and pulmonary circulations (47–52).
The overall goals of this study were thus to evaluate the changes in EC-SOD expression and activity in the lung in response to hypoxia; to determine if lung-specific overexpression of EC-SOD would protect against PH and pulmonary vascular remodeling; and, if so, to determine if any of the protective effects might be achieved through suppression of Egr-1/TF pathways. Our approach was to utilize wild-type (WT) and transgenic mice overexpressing human EC-SOD in the lung and assess the effects of hypoxia on physiological, pathological, and biochemical parameters indicative of PH. We present new data demonstrating a loss of EC-SOD activity in the lung after exposure to chronic hypoxia, a protective effect of lung EC-SOD overexpression on chronic hypoxic PH and vascular remodeling, and a novel role for extracellular O2·− in the hypoxic induction of Egr-1 and the downstream target TF in the murine lung.
Mouse strains.
Animal studies were approved by the Institutional Animal Care and Use Committees. We tested WT C57/BL6 mice and heterozygous transgenic mice bred in the C57/BL6 background that overexpress human EC-SOD (TG). The human EC-SOD transgene is driven by the SP-C promoter to generate overexpression specifically in the lung, and, similar to native EC-SOD, contains the sequences that enable extracellular secretion of the protein (10).
Chronic hypoxic PH.
To produce chronic hypoxia, 8-wk-old mice were placed in a hypobaric hypoxia chamber at a simulated altitude of 18,000 ft (395 mmHg) for up to 35 days. In chronically hypoxic mice exposed to 35 days of hypoxia, along with their age-matched normoxic controls, the development of PH was assessed by right ventricular (RV) pressure measurements. Mice were lightly sedated with intramuscular ketamine (50 mg/kg) and xylazine (10 mg/kg), and RV pressure was measured in a closed chest via a direct RV puncture. A 25-gauge needle attached to a pressure transducer was introduced into the RV, and placement was confirmed by a live pressure tracing. Pressures were measured using the Cardiomax III Cardiac Output Computer (Columbus Instruments) connected to a Dell laptop running the Cardiomax III program v2.10. Systolic RV pressures (RVSP) were monitored for 30 s and averaged every 10 s to account for beat to beat variability. The RVSP measurements for each mouse were compared between different experimental conditions. At the end of the experimental protocol, mice were euthanized with intraperitoneal pentobarbital (150 mg/kg).
Lung tissue was harvested at several time points from normoxic and chronically hypoxic mice. Before harvesting lung tissue, lungs were flushed with 10 ml of cold saline. The right lung was flash-frozen for mRNA, protein, and activity assays. The left lung was inflation-fixed at 20 cmH2O pressure in 4% paraformaldehyde for 20 min and then dissected from the chest cavity and placed in 4% paraformaldehyde at 4°C overnight. Lungs were then transferred to 70% ethanol, paraffin embedded, and sectioned for immunohistochemistry.
SOD activity measurements.
In lung homogenates, EC-SOD was separated from intracellular SODs (Cu,Zn SOD and MnSOD) using a concavalin A sepharose column as previously described (33). EC-SOD and intracellular SOD activity levels were measured using a commercially available SOD assay kit (Dojindo Molecular Technologies). Briefly, this kit utilizes a water-soluble tetrazolium salt, WST-1 [2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfo-phenyl)-2H-tetrazolium, monosodium salt], to produce a water-soluble formazan dye upon reduction with a superoxide anion, detectable by a colorimetric assay. The rate of WST-1 reduction by superoxide anion is linearly related to SOD concentrations between 0.1 and 10 U/ml of SOD; thus, activity is calculated based on a standard curve generated with xanthine oxidase and SOD. SOD activity data was expressed as units of SOD activity per gram of tissue.
Protein expression.
Lung protein homogenates (cytosolic and membrane proteins) and nuclear protein extracts were isolated from lung tissue using a commercially available kit (NE-PER, Pierce Biotech). Proteins (10 μg nuclear protein extracts or 30 μg lung protein homogenates) were separated by gel electrophoresis and transferred to PVDF membranes as previously described. Blots were blocked with 5% milk in TBS and probed with primary antibody for 1 h followed by an appropriate HRP-conjugated secondary antibody. Blots from the lung protein homogenates were probed with the following primary antibodies: polyclonal rabbit anti-mouse EC-SOD antibody (1:1,000) to detect endogenous EC-SOD, polyclonal rabbit anti-human EC-SOD antibody (1:5,000) to detect the human EC-SOD transgene (kind gift of Dr. James Crapo, National Jewish Medical and Research Center, Denver, CO), and polyclonal rabbit TF antibody (1:500; American Diagnostics, Stamford, CT). Each blot was stripped (Restore Plus Western Blot Stripping Buffer, Pierce) and reprobed with β-actin antibody (1:6,000) for protein normalization. Blots from nuclear protein extracts were probed with a rabbit polyclonal Egr-1 antibody (1:1,000; Santa Cruz Biotechnology, Santa Cruz, CA) and rabbit polyclonal NAB2 antibody (1:5,000, Active Motif). These blots were stripped and reprobed with the nuclear loading control TATA Binding Protein (Abcam, Cambridge, MA) for normalization. Blots were developed with ECL plus (GE Healthcare) and bands quantified by densitometry. Normalized values were expressed relative to mean normoxic WT protein expression.
Paraformaldehyde-fixed and paraffin-embedded lung sections were immunostained as previously described with the following antibodies: polyclonal rabbit anti-mouse EC-SOD antibody (1:1,000), polyclonal rabbit anti-human EC-SOD (1:500), mouse monoclonal α-smooth muscle actin antibody (α-SMA; 1:100, Clone 1A4), and rabbit monoclonal Ki67 antibody (1:200 Lab Vision NeoMarkers, Ki67 immunoperformed by IHC Services, Aurora, CO). In brief, lung sections were deparaffinized with citrisolve and rehydrated in graded ethanol. Slides were treated with 3% hydrogen peroxide in methanol for 5 min to block endogenous peroxidase activity and treated with 0.01% pronase for 20 min for antigen retrieval. The block, secondary antibody, and ABC reagent were provided in the RTU Universal Elite ABC Kit (Vector Laboratories) for polyclonal rabbit antibodies and the Mouse-on-Mouse kit (Vector Laboratories) for the mouse monoclonal α-SMA antibody. The slides were developed with ImmPact DAB diluent (Vector Laboratories) and counterstained with hematoxylin. Slides were dehydrated with ethanol followed by citrisolve before placement of coverslips. Tissue sections were examined by light microscopy and photographed using a final magnification of ×200. The number of small vessels (<50 μm) with positive α-SMA staining was counted in 10 peripheral high-powered (×20) lung fields to evaluate for muscularized small pulmonary vessels. The number of proliferating Ki67-positive cells within the vessel wall was measured by counting Ki67-positive cells within the intimal and medial layer of muscularized pulmonary arteries between 50 and 150 μm. At least seven vessels were counted for each mouse lung. The analysis of α-SMA and Ki67 staining was evaluated by an investigator blinded to treatment group and genotype.
GSH/GSSG measurements.
Oxidative stress in lung tissue was evaluated using a commercially available glutathione assay kit (Cayman Chemical, Ann Arbor, MI). The kit measures GSH by an enzymatic recycling method using glutathione reductase for the quantification of total tissue glutathione. Fifty milligrams of lung tissue was homogenized in MES buffer (MES, 0.1 M phosphate, and 2 mM EDTA, pH 6.0) and centrifuged at 10,000 g for 15 min at 4°C. The supernatant was removed and deproteinated for analysis (Centrifugal Filter Units, 10,000 MW Cut-Off, R&D Systems, MN). We tested both total glutathione in lung homogenates after reducing GSSG to GSH as well as GSSG alone according to the manual instructions and calculated the ratio of reduced GSH to oxidized GSSG.
Picrosirius red staining of collagen.
The impact of EC-SOD overexpression on chronic hypoxia-induced pulmonary vascular remodeling was further assessed by staining collagen in lung sections with picrosirius red (6). Lung sections were deparaffinized with citrisolve and rehydrated in graded ethanol followed by deionized H2O. Sections were treated with 400 μl of 0.2% phosphomolybdic acid (Electron Microscopy Services, Hatfield, PA) for 5 min followed by 400 μl of 0.1% Sirius Red in saturated picric acid (Electron Microscopy Services) for 90 min at room temperature. The tissue sections were washed with 0.01 N HCl and rinsed with 70% ethanol. The sections were counterstained with hematoxylin. The slides were then dehydrated in graded alcohols followed by citrisolve, and after drying, mounting media and coverslips. Pulmonary arteries adjacent to the junction between the terminal bronchioles and alveolar ducts were photographed by light microscopy to allow visual comparisons between similar sized mid-sized blood vessels.
mRNA expression.
Mouse EC-SOD, Egr-1, and TF mRNA expression was determined by semiquantitative RT-PCR. Briefly, total RNA was isolated from the lung using TRIzol reagent (Invitrogen, Carlsbad, CA). One microgram of RNA was reverse-transcribed using a 2:1 mixture of oligo dT to random hexamer primers and Superscript III enzyme (Invitrogen) according to Invitrogen instructions. mRNA expression was amplified for 30 cycles by the PCR using mouse specific primers for Egr-1 and TF. Primers were EC-SOD forward: 5′-GCACTTCCACAGACC CAGAT-3′, reverse: 5′-GGTGAGGGTGTCAGAGTGGT-3′; Egr-1 forward: 5′-GCA GAT CTC TGA CCC GTT CGG -3′, Egr-1 reverse: 5′-CCG AGT CGT TTG GCT GGG ATA-3′; TF forward: 5′-GCC GGT ACC CAT CAC TCG CTC CCT CCG-3′, TF reverse: 5′-TTC CTC CGT GGG ACA GAG AGG ACC TTT G-3′. Products were run on an agarose gel, and band intensity was measured by densitometry. Egr-1 and TF amplicons were normalized to β-actin amplicons, and normalized values were expressed relative to normoxic WT mRNA expression.
Reagents, unless specified, were obtained from Sigma/Aldrich Chemical (St. Louis, MO).
Data analysis.
Data were expressed as means ± SE. Comparisons were made between normoxic and hypoxic mice and between genotypes by one-way ANOVA followed by Bonferroni post hoc analysis using Prism GraphPad software. Statistical significance was defined as P < 0.05.
Lung EC-SOD activity and expression in chronic hypoxia.
We measured the effect of chronic hypoxia on lung EC-SOD protein activity, expression, and localization in WT mice. The impact of hypoxia on endogenous EC-SOD activity was determined in WT mice at early (1-day) and late (35-day) time points. Lung EC-SOD activity was increased following the 1-day exposure of WT mice. However, by 35 days of hypoxia, lung EC-SOD activity was decreased to 60% of baseline activity (Fig. 1A).
In contrast, the normoxic EC-SOD TG mice showed an approximately five- to sixfold increase in lung EC-SOD activity levels compared with WT mice, similar to our previously published report (10). The EC-SOD activity levels in the EC-SOD TG mice remained elevated and did not significantly change in response to either a 1-day or 35-day exposure to hypoxia. After 35 days of hypoxia, the EC-SOD activity in the EC-SOD TG mice was sevenfold greater than WT mice due to the decreased activity levels measured in WT mice at this late time point (Fig. 1B). Intracellular SOD (SOD1 and SOD2) activity remained similar in both strains of mice under normoxia (521 ± 40 U/g tissue in WT vs. 464 ± 195 U/g tissue in EC-SOD TG, n = 5 each, P > 0.05) with no significant change following exposure to 35 days of hypoxia.
Although we detected a small increase in EC-SOD activity at 1 day of hypoxia in the lungs of WT mice, we measured a decrease in GSH/GSSG ratio after 1 day of hypoxia, indicative of increased oxidative stress in response to hypoxia (Fig. 2). In contrast, in mice overexpressing EC-SOD, there was an increased GSH/GSSG ratio compared with WT mice at baseline that did not significantly decrease in response to hypoxia (Fig. 2).
We evaluated changes in EC-SOD protein expression and localization using antibodies specific either for endogenous mouse EC-SOD or the human EC-SOD transgene. Endogenous EC-SOD protein expression in WT mice did not significantly change after a 1-day exposure to hypobaric hypoxia (Fig. 3A, left). After 35 days of hypoxia, in concordance with the decrease in EC-SOD activity, there was a decrease in EC-SOD protein expression (Fig. 3B, left). We tested EC-SOD mRNA expression in the WT lungs after 1 and 35 days of hypoxia to evaluate the level of regulation. mRNA levels, evaluated by the densitometry of the EC-SOD signal relative to β-actin, did not change after 1 or 35 days of hypoxia (P > 0.05 by 1-way ANOVA, n = 4, data not shown). In the EC-SOD TG mice, baseline protein expression of the mouse EC-SOD was similar to normoxic WT mice. The expression of endogenous mouse EC-SOD in the EC-SOD TG mice did not change after 1 day (Fig. 3A, right) or 35 days of hypoxia (Fig. 3B, right). Protein expression of the human EC-SOD protein in EC-SOD TG mice did not change in response to hypoxic exposure (Fig. 3C).
EC-SOD was localized predominantly within and around pulmonary arteries of hypoxia-exposed WT mice, similar to the localization described previously in lungs of normoxic mice, although the intensity of staining decreased in the 35-day hypoxic lung. In the EC-SOD TG mice, localization of endogenous mouse EC-SOD was similar after both 1 and 35 days of hypoxia (Fig. 4A). As the human EC-SOD antibody does not recognize mouse EC-SOD, the WT mouse lung did not stain for human EC-SOD. Human EC-SOD protein was distributed through the lung parenchyma as well as around pulmonary arteries in 1-day (not shown) and 35-day hypoxia-exposed EC-SOD TG mice (Fig. 4B).
Overexpression of EC-SOD protects against chronic hypoxic PH.
To establish whether EC-SOD and extracellular O2− contribute to the development of chronic hypoxic PH, we tested whether lung-specific overexpression of EC-SOD imparted protection against increases in RVSP, an indirect measure of pulmonary artery pressures. WT and EC-SOD TG mice had similar baseline RVSP under normoxic conditions. WT mice exposed to 35 days of hypobaric hypoxia developed PH shown by an increase in RVSP. In contrast, although RVSP increased from baseline in EC-SOD TG mice, the degree of PH was significantly less in EC-SOD TG mice exposed to 35 days of hypoxia (Fig. 5).
Overexpression of EC-SOD attenuated chronic hypoxic pulmonary vascular remodeling.
A pathognomonic feature of chronic hypoxic PH is the remodeling of small pulmonary vessels. This process is characterized by several changes including cell proliferation within the pulmonary artery wall, muscularization of small, previously non-muscularized vessels, and deposition of extracellular matrix protein, including collagen in the pulmonary arteries. We found that overexpression of EC-SOD attenuated each of these markers of pulmonary vascular remodeling. We used Ki67 nuclear staining as a marker for cell proliferation. We detected an increase in proliferating cells within the medial and intimal layers of similar sized pulmonary arteries (50–200 μm) of WT mice in response to a 3-day exposure to hypoxia (Fig. 6, A and B). In the EC-SOD TG mice, there was a similar number of proliferating cells in the pulmonary arteries under normoxic conditions and at 1 day of hypoxia compared with WT mice. Following 3 days of hypoxic exposure, an increase in the number of proliferating cells was observed, but the number of proliferating cells remained significantly less than observed in the WT mice (Fig. 6, A and B). Interestingly, we also observed a marked increase in Ki67 immunoreactivity throughout the lung parenchyma in response to 1 and 3 days of hypoxia in WT mice. This early increase in Ki67 staining was significantly decreased in the EC-SOD TG mice. In addition, proliferation in the lung was dramatically decreased at 35 days of hypoxia in both strains of mice (Fig. 6A).
The appearance of newly muscularized small pulmonary vessels was evaluated by immunohistochemical staining for α-SMA. We found, as expected, a significant increase in the number of small (<50 μm) vessels positive for α-SMA in the lungs of 35-day hypoxic WT mice. The number of α-SMA-expressing small (<50 μm) pulmonary vessels measured in WT mice at 35 days of hypoxia significantly decreased in the lungs of hypoxia-exposed EC-SOD TG mice (Fig. 6, C and D).
Another aspect of vascular remodeling is the deposition of collagen around pulmonary arteries, which is most obvious in the mid-sized pulmonary arteries, which normally contain small amounts of collagen. Prominent increased collagen deposition, shown by picrosirius red staining, was noted in the lungs of chronically hypoxia-exposed WT mice. A decrease in staining was observed in similar sized vessels at the terminal bronchiolar/alveolar duct junction in the EC-SOD TG littermates (Fig. 6E).
EC-SOD regulates hypoxia-induced pulmonary Egr-1 mRNA and protein expression.
Once we established that EC-SOD overexpression protected against chronic hypoxic vascular remodeling, we tested whether EC-SOD overexpression could prevent the hypoxic upregulation of a transcription factor (Egr-1) and a downstream target, TF, thought to be critical to the remodeling process. In WT mice, exposure to 1 day of hypoxia increased lung Egr-1 gene expression by 4.4-fold, along with a concomitant increase in nuclear Egr-1 protein by 2-fold (P < 0.05) (Fig. 7, A and B, left). In striking contrast to the WT mice, no upregulation of Egr-1 mRNA or nuclear protein was observed in EC-SOD TG mice (Fig. 7, A and B, right).
We provided further supporting evidence that EC-SOD overexpression blocks Egr-1 expression by testing for the coinduction of the NGF1-A binding protein, NAB2. It has been shown in other injury models that Egr-1 can induce the expression of its own corepressor molecule, NAB2. The binding of NAB2 to Egr-1 in turn represses transcription of Egr-1 target genes, including Egr-1 itself, to establish a negative feedback loop for Egr-1 activity (18, 25, 42, 45). After 1 day of hypoxia, when Egr-1 expression increased in WT mice, we also detected a corresponding increase in nuclear NAB2 protein. In contrast, in the mice overexpressing EC-SOD that did not exhibit hypoxic induction of Egr-1, the corepressor protein NAB2 also remained low (Fig. 7C).
EC-SOD regulates the hypoxic induction of TF, a downstream gene target of Egr-1.
TF is an important downstream gene target of Egr-1 that is upregulated in the lung in response to hypoxia and initiates a procoagulant state that contributes to pulmonary vascular remodeling (51). Published studies in transgenic mice lacking Egr-1 have already demonstrated the critical link between Egr-1 and TF; therefore, the current studies were designed specifically to examine the impact of EC-SOD overexpression on TF expression. Lung TF mRNA and protein both increased in WT mice exposed to 24 h of hypoxia (Fig. 8, A and B, left). Notably, hypoxia did not induce TF mRNA and protein in the lungs of EC-SOD TG mice (Fig. 8, A and B, right).
The purpose of this study was to evaluate whether overexpression of EC-SOD, the only known extracellular enzymatic defense against O2·−, was protective in chronic hypoxic PH. We report the novel findings that: 1) chronic hypoxia alters the expression and activity of EC-SOD in the lung, 2) overexpression of EC-SOD limited the development of chronic hypoxic PH and pulmonary artery remodeling, and 3) overexpression of EC-SOD prevented the early hypoxic induction of lung Egr-1 and its corepressor NAB2 as well as the Egr-1 downstream gene target, the procoagulant regulator TF. To date, this is the first study to report the changes of EC-SOD in the chronically hypoxic lung and to show that overexpression of EC-SOD blunts the development of chronic hypoxic PH and attenuates redox-sensitive gene expression in the lung.
An increasing number of studies support the concept that O2·− generation increases in the lung in response to low oxygen tension and contributes to chronic hypoxic PH and remodeling (11, 20, 21, 28). This study provides additional evidence of hypoxia-induced oxidative stress by demonstrating a decrease in the ratio of reduced glutathione to oxidized glutathione within the lungs of WT mice exposed to hypoxia. Several enzymatic sources of ROS have been identified in hypoxic lung including NOX2 and NOX4 containing NADPH oxidases and xanthine oxidase (17, 21, 28, 31). Studies in models of PH other than chronic hypoxia also demonstrate a role for ROS via NADPH oxidase and uncoupled endothelial nitric oxide synthase in disease pathogenesis (15). Furthermore, in a number of rat studies, treatment with different antioxidant strategies, including dimethylthiourea, allopurinol, N-acetylcysteine, or tempol, attenuated the development of chronic hypoxic PH (7, 21, 26). This study extends these studies by focusing specifically on the impact of hypoxia on EC-SOD and the protective effects of EC-SOD overexpression in chronic hypoxic PH.
In the present study, we measured changes in EC-SOD activity, expression, and localization in response to hypoxia and defined how the EC-SOD overexpressing transgenic mice differ from their WT counterparts under hypoxic conditions. We observed a modest increase in lung EC-SOD activity in WT mice exposed to 1 day of hypoxia without a concomitant increase in protein expression. This posttranslational change in EC-SOD activity is likely an early adaptive response to increased extracellular O2·− production as evidenced again by the change in the redox state of the lung at this early time point. Exposure to hypoxia for 35 days resulted in a significant decrease in EC-SOD activity and protein expression. This finding is consistent with hyperoxic lung injury in adult mice, which also demonstrated impaired EC-SOD activity and protein expression in response to the altered oxygen tension (38). In this study, we detected a corresponding decrease in EC-SOD protein expression in WT mice. EC-SOD mRNA did not decrease following 35 days of hypoxia, suggesting that the decrease in EC-SOD activity is regulated at the translational and posttranslational level. EC-SOD activity may be regulated at the posttranslational level by cleavage of the heparin binding domain, loss of copper incorporation, or protein modification such as nitration (28, 38). Further studies are indicated to better understand the mechanisms responsible for EC-SOD expression and activity. The EC-SOD TG mice exhibited high EC-SOD activity levels that did not change in response to hypoxia, with the human EC-SOD transgene accounting for 75–87% of the lung EC-SOD activity. The activity levels in EC-SOD TG mice were substantially higher than in WT mice at both 1 and 35 days of hypoxia. The SP-C promoter that drives human EC-SOD expression in the transgenic mice is not known to be regulated by hypoxia. These mice had a higher portion of reduced to oxidized glutathione at baseline compared with WT mice with no significant change in the redox state of the lung in response to hypoxia, further demonstrating the protective effects of EC-SOD overexpression. The marked differences in EC-SOD activity without a difference in intracellular SOD activity make the use of these two strains, the WT mice and EC-SOD TG mice, useful to test the contribution of extracellular O2·− to the development of chronic hypoxic PH.
Mice overexpressing or lacking EC-SOD have been utilized in models of vascular and lung diseases associated with fibrosis including bleomycin-induced lung fibrosis, viral pneumonia, and hyperoxic lung injury to demonstrate a role for extracellular O2·− in disease pathogenesis (1, 2, 5, 8, 10). The protective effects of EC-SOD have been attributed both to its ability to limit extracellular reactions of O2·− and to prevent the inactivation of NO by O2·−, thus preserving NO bioactivity. Chronic hypoxia leads to both an increase in O2·− and impaired NO production despite increased eNOS expression, providing a strong rationale to test the impact of EC-SOD on chronic hypoxic PH. We now demonstrate that lung-specific EC-SOD overexpression significantly attenuates the development of PH and pulmonary vascular remodeling in response to chronic hypoxia. Interestingly, in this mouse strain, the human EC-SOD transgene is selectively expressed by type II alveolar epithelial cells, although we still observed protection within the pulmonary circulation. There are several possible explanations for this observation. First, although the human EC-SOD transgene is synthesized within type II cells, we found that it is secreted into the extracellular compartment in the hypoxia-exposed lung and is detectable throughout the lung, including the vessel wall. This enables EC-SOD to directly scavenge extracellular O2·− generated by the pulmonary artery. Second, since other lung cells can influence pulmonary vascular function, it is also possible that EC-SOD protects the pulmonary circulation through its effects on other cells within the lung including both epithelial cells and inflammatory cells. It is known that bronchial tissue can influence vascular tone, and factors such as VEGF produced by the epithelial cells influence adjacent vascular cell proliferation and differentiation (4). Interestingly, we observed that changes in the lungs of hypoxia-exposed mouse, including cell proliferation and collagen deposition, were not exclusively localized to the pulmonary artery, as there were also visible changes within the lung parenchyma. It is possible that specific vascular smooth muscle cell overexpression of EC-SOD would have a different effect on chronic hypoxic PH. While this study focused on the pulmonary circulation, further investigations are needed to more fully understand the entire pulmonary response to chronic hypoxia and the contribution of extracellular O2·− to these processes.
There is a large body of literature that demonstrates the role of ROS in the regulation of gene expression, although few studies have examined the contribution of O2·− generated in the extracellular compartment. Therefore, as the first step to examine how overexpression of EC-SOD might protect the hypoxia-exposed lungs from the development of PH, we tested the impact of EC-SOD overexpression on the hypoxic induction of the redox-sensitive transcription factor Egr-1, along with its downstream gene target, TF. Egr-1 is increasingly recognized as a critically important master regulator of genes that contribute to the diverse cardiovascular and pulmonary diseases, including hypoxic PH (24). Our observation that Egr-1 and TF are increased in the hypoxia-exposed WT mouse is consistent with a series of published studies from our laboratory and others that also report an increase in Egr-1 within days of hypoxic exposures and furthermore confirm a role for Egr-1 and TF in fibrin deposition in the hypoxic pulmonary vasculature (3, 12, 22, 49–51). A key observation in this study was that the hypoxic induction of Egr-1 and TF at 1 day in WT mouse lung did not occur in mice overexpressing EC-SOD. We also reported an increase in NAB2 in the WT lungs, but not EC-SOD TG lungs, in response to hypoxia. The upregulation of NAB2 in response to hypoxia has not been previously reported but is consistent with other models in which NAB2 increases along with Egr-1 (18, 25, 42, 45). As NAB2 is also a downstream gene target of Egr-1, our data indicate that the pathway to limit Egr-1 activity, by upregulating its own corepressor molecule to suppress transcriptional activity, occurs simultaneously with the upregulation of downstream targets that promote vascular remodeling (e.g., TF). The protective effects of EC-SOD on Egr-1 expression could be mediated by either decreased O2·− levels or increased NO bioavailability and are the focus of new studies.
We limited this study to one transcription factor and these two downstream targets, TF and NAB2, to provide proof of principle, although we recognize that other transcription factors as well as other Egr-1-dependent genes are probably also important. Our laboratory previously reported in pulmonary artery adventitial fibroblasts isolated from chronically hypoxic neonatal calves that Egr-1 regulated growth-related genes, cyclin D and EGFR, and that inhibition of Egr-1 in this cell culture model prevented hypoxia-induced fibroblast growth (3). Although we did not measure genes associated with proliferation, we did observe changes in cell proliferation early in response to hypoxia in the WT, but not EC-SOD TG, mice, which could be due in part to the changes in Egr-1. Recently, in other models of lung disease, several notable Egr-1 responsive gene targets have been implicated in lung injury including proinflammatory cytokines, bone morphogenetic protein receptor, and TGF-β (16, 19, 32, 40). These studies are highly interesting due to the link between these particular genes and human PH and provide the rationale for future studies of other downstream gene targets of Egr-1 in hypoxic PH.
In summary, the development of chronic hypoxic pulmonary vascular remodeling and PH was attenuated in mice overexpressing EC-SOD. The early hypoxic induction of a transcription factor, Egr-1, and its downstream target, TF, which contribute to hypoxic pulmonary vascular remodeling, were significantly blocked in the lungs of EC-SOD TG mice. The corepressor protein NAB2 was also upregulated in hypoxia-exposed WT mice but not EC-SOD TG mice. Our new data thus support our hypothesis that the generation of extracellular O2·− in hypoxia contributes to the development of PH and upregulates redox-sensitive genes relevant to pulmonary vascular remodeling. These data provide new insight into the role of ROS on chronic hypoxic PH. These data also provide a foundation for future investigations to test new therapeutic interventions with novel antioxidant strategies that target extracellular O2·− in the lung.
This work was funded by National Heart, Lung, and Blood Institute (NHLBI) Grant HL-086680 and March of Dimes #6-FY06-316 (both to E. Nozik-Grayck) and NHLBI Grant HL-084923-01 (to K. R. Stenmark).
We thank Dr. Sonia Flores for constructive feedback and Sebastian Albu and Erin Bolivar for excellent technical assistance.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2008 the American Physiological Society


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The closed beta servers are currently offline. They are going to be down for an undisclosed period of time until another phase starts.
Check out this thread for more information:
I'm not sure if it will help or not since the closed beta is offline now, but try clearing your Internet Explorer cache and cookies and then restarting the launcher. You won't be able to log into the game though until another testing phase starts.


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Did I really climax three times?
From the guy who just stopped being a virgin twenty minutes ago?
Ok so maybe it wasn't twenty minutes ago but the guy was definitely inexperienced when it came to sex.
Tyler was a beautiful man but he was quiet and bordering on shy. His close brush cut, business casual attire, and modest build made him fade into the background. If you weren't paying attention you would walk right past him as just another guy in the office.
When had that changed?
Shonda rolled over to face the wall and felt a soreness in her vagina. That good hurt when a man puts in work into the sex like it's his 9-5 paid position.
Her thoughts screaming a mile a minute as Tyler wrapped his arm around her and squeezed. She didn't even care that her freshly pressed hair was now a mess.
Damn. How did they even get here?
About six months ago she started working at XY Corp. It was her dream job in marketing and she got to work with amazing people. She encountered tyler one day in the break room reading the paper and sitting all alone. She's not sure what compelled her to go over there and speak to him but from that day forward they'd do lunch at least three times a week.
He never made a pass at her and she almost decided he might have been gay.
One day the lunch conversation turned a bit more intense as they discussed a scandalous new office romance. Word in the copier room was that the CEO was sleeping with the head of advertising and they'd even gotten it on in his office!
Tyler looked noticeably uncomfortable as Shonda told the story in full animation – hand gestures and little dirty comments included.
"What's wrong?"
"I just, I don't know what to say. I never really had sex more than twice in my life so I'm not sure how to really add to this conversation."
"WHAT?" Shonda sat back in noticeable surprise and crossed her arms,unknowingly accentuating her perky c-cups and stretching the limits of her buttons.
She adjusted in her seat when she noticed Tyler's eyes drop to her bust line.
So maybe he wasn't gay after all.
His innocence was intriguing. She felt a moisture build between her legs and a familiar tightness form.
She's heard her friends brag about "teaching a guy some things!" and she'd never had that opportunity because she always slept with more experienced men. Tyler would be her chance to experiment.
A few days later Shonda lay on her back as Tyler stood over her near the edge of the bed. They'd both devoured a half bottle of wine and it was time for Shonda to really SHOW him what sex was all about.
His penis hung hard and heavy. Apparently under all that nerd exterior he'd been hiding a really nice, girthy, long, package.
She massaged her breasts and ran her fingers through her hair. He just watched her with lust and passion.
Come here, she whispered.
He obediently got into the bed. She turned on her side and reached down to massage his hardness. To her surprise he kissed her on the forehead and began a slow trail down her face…to her breast.
He was sooooo giving? She couldn't really describe it but each kiss was methodical.
He whispered for her to relax.
"Let me show you how your body should be treated…" he slid his hand behind her back and pulled her closer.
His tounge traced down her belly button and he spent what felt like an eternity simply caressing her backside, kissing and sucking her thighs, and brushing past her wetness. She thought she'd scream.
He finally began to taste her. His tongue was strong and slow. She put her head back and tried to relax but soon found herself tugging at his ears. She tried to scoot back because it felt so good and wasn't she supposed to be teaching him? He locked his arms around her waist and pinned her to the bed. Thrusting his tongue in and out, licking all the right spots, she climaxed. He looked up at her and massaged her breast for a bit.
When she her breathing returned to normal he was still down there. Caressing and massaging and…was he licking again? This time her climax included a small puddle that threatened to drown him. He got up, toweled
off, and came back to rest on top of her.
Ok, he'd taken the gold medal in giving head but now it was her turn to show him.
He slid inside her and she took a deep breath in as he looked in her eyes. He called her beautiful and started a slow thrust. Deeper and deeper. She couldn't get away from his grasp and he had her legs by her ears.
Shonda didn't even know her legs could go that far.
He turned her on her belly and lifted her backside. He rubbed down her back and gently pulled her hair as he pressed harder and harder into her. She tried to push back but he was giving her so much that she just had to take it. He rubbed her clitoris at the same time and before she knew it she was waking up the neighbors with yet another orgasm.
He finally returned her to her back and kissed her intensely as he finally climaxed.
When he returned from the bathroom she started to get up as well to go clean herself up. But Tyler had already brought out a warm wash cloth for her. He wiped her down in a way that gave her good chills.
Now with his arms wrapped around her she couldn't help but wonder where he learned all this from. I guess when you don't have sex that often you learn to give it all you got when you do.
Shonda had started the night ready to teach him. Instead, the shy quiet guy reading a news paper in the break room, had become her ultimate lover.
And now she was ready to learn.


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Stock Graphic >> Web Element - pack 85 pictures of Paris 123831 by robcar @ CreativeMarket
File Size: 491.87 MB; DPI: 72 DPI.
The pack consists of 85 photos of the city of lights and Versailles. Photos are perfect for web design or funds are in .jpg format
Keywords: architecture, invalids, city, beauty, blue, sightseeing, invalides, symbol, historic, tall, district, metal, europe, landscape, eiffel tower, international, exterior, vintage, outdoors, beautiful.


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Forgot your password? Enter the email address you signed up with and we will send you a link to create a new password.
Your password reset has been submitted to the address provided.
Design plus words, letters, and/or numbers
Household and kitchen machines and equipment, namely, electric cleaning equipment for household use, namely, vacuum cleaners; parts of all aforementioned goods
Business in Munich, DE


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GraphicRiver Trifold Brochure Mock-Up Pack 7003859
Photoshop \ Stock Graphic \ Product Mock-Ups \ Print \ Brochures
Brochure \ Trifold \ Package \ Object \ Slicing \ Designed \ Standard \ Different
Stock Graphic - Trifold Brochure Mock-Up Pack 7003859 by mariokulas @ GraphicRiver
Photoshop \ Stock Graphic \ Product Mock-Ups \ Print \ Brochures
- 2 .PSD files for easy slicing your pre-designed brochure
- 12 different trifold brochure mock-ups (8 horizontal and 4 vertical view)
- Smart object based for easy editing
- Realistic dimensions and high resolution (3200×2400px / 300 PPI)
- Fully layered with separated shadows and background
- Photoshop CS4 or Later
- Optimized for ISO A4 size, EU standard
(however you can insert any image size via smart object and adjust it to fit)
- HELP FILE with Easy-to-Understand Instructions
ADDITONAL INFORMATION: This set of trifold brochure mock-ups is for standard European A4 size (297×210 mm / 11.7×8.3 inch). If you donЎдt know how to precisely slice your pre-designed image into 3 parts, donЎдt worry. With this package you also get two .PSD files for easy, fast and precise slicing your image into 3 parts to fit all of 12 trifold brochure mock-ups.
Items that you get in the package are.
- 2 .PSD files for easy slicing your pre-designed image
- 12 different .PSD files of trifold brochure mock-up
- Help file with Easy-to-Understand Instructions
* Fonts on this image preview are not included in the package.
IF YOU LIKE THIS ITEM, PLEASE DONЎдT FORGET TO RATE
High Resolution: Yes; Layered: Yes; Graphics Files Included: Photoshop PSD; Minimum Adobe CS Version: CS4; Pixel Dimensions: 3200x2400.
Night Sky Party Flyer; Yoga Flyer; Orthodontist / Dental Trifold Brochure; 12 Dental Icons; Square Brochure Mock-Up Pack ; Trifold Brochure US Standard; Gate Fold Brochure Mock-Up Pack.
Keywords: 300 dpi, 300 ppi, 3d, a4, branding, brochure, brochure design, eu standard, high resolution, mock-up, package, photorealistic, presentation, print mockup, real, render, set, showcase, smart object, template, tri-fold, trifold brochure, visualization.


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This is one of my favorite ways to make cranberry relish that's perfect for Thanksgiving. It's so fresh and vibrant… and so colorful and gorgeous, that I love to place it in mason jars and give it as gifts during the holiday season.
There's no cooking involved, and all the fruit is raw. I love that I can easily whip [Read more…]


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Contact: Lise LeMay-Corlett
Address: 1561 Flos Rd. 8 W
Elmvale, ON
Phone: 705-322-8181
Email: email@example.com
Website: www.sevensummits.ca
Seven Summits is a boarding and lesson barn that provides a calm environment for horses and riders to enjoy each others company. Situated on 89 acres we offer 20+ acres of pasture, a large outdoor jumper ring, full size dressage ring, sand schooling ring, round pen and trails. We take part in many shows but also have many riders who just enjoy the pleasure of equine company. We are a "no pressure" environment.


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Welcome to Hollywood Feed!
Fast Free Shipping on orders over $49 - Learn More
Have a question? Ask a specialist!
Or Call 888-816-8691
You know how a hug just makes you feel better? It turns out there is science to back that up. Studies show moderate to deep pressure reduces anxiety and promotes relaxation in both humans and animals. The Thundershirt applies gentle, constant pressure to your pet similar to swaddling a baby and has an 80 percent success rate in soothing stressed pets in homes and shelters.


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Northwest Olympic Athletes
The Great Pacific Northwest is being represented in Sochi this year by eight athletes and one very...
2014 Olympic Winter Games
The Opening Ceremonies for the 2014 Olympic Winter Games will begin on February 7th in Sochi,...
< Underage Gonzaga Students Trying to Buy Alcohol
CASTLEFORD, Idaho (AP) - A south-central Idaho dairy farmer who
Apr 30, 2012 06:21
Category: General News
Category: General News
Solar Panel Powered Dairy Farm
CASTLEFORD, Idaho (AP) - A south-central Idaho dairy farmer who
put up solar panels to save on energy costs is drawing the
attention of other dairy farmers in the region. Dan De Kruyf,
co-owner of Kowz R Us Dairy, said the 30 solar panels heat more
than 400 gallons of water used to sanitize the dairy's holding
tanks. Most of the $53,000 cost of the solar panels was paid for
through grants.


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Hidalgo Weathered Log & Whitetail Deer Antler Table LampA naturally rustic fit with a log furniture theme, real antler lighting adds decor and practicality to any country cabin or lodge. This Weathered Log & Whitetail Deer Antler Table lamp will make a useful piece of rustic decor to your log cabin office or den. This antler lamp features an antler finial, and a genuine rawhide shade.
All electrical wiring is artfully hidden within the log. All antler lighting and parts are UL listed. All necessary mounting hardware is included.
All of our antler lighting is made from naturally shed antlers collected from the wild - no animals are ever harmed.
Approx. dimensions: Approx 12" at the base and 21" to the top of the shade.
Click the link to view our full selection of rustic handcrafted lamp shades !
Because most antler lighting is handmade upon order, these items generally ships in 4-8 weeks. As with most of our rustic furniture and accessories, this item has free curbside delivery to the lower 48 states!