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(Diablo Eyelash Sage) Small, eyelash-like hairs on the edge of its leaves give this Mexican native part of its name. It earns "Diablo," which means "devil" in Spanish, from the two yellow stamens that stand up out of each flower like horns.
This compact, gently mounding Salvia spreads gradually by underground stolons. Its rich red flowers are darker in full sun and paler in partial shade. It blooms from early summer to late fall and is a delightful addition to a mixed border.
These are species whose stems never develop a woody character and that either die to the ground or loose leaves and become unsightly at the end of a growing season. This group includes both hardy and tender types. Many of the tender forms are grown as annuals in cold winter areas.
Pruning is both an art and a science. It takes practice, experience and learning from your mistakes to become a proficient pruner. The pruning information about this plant should be considered as a guideline for getting started. Your particular climate, soils, watering and fertility schedules, sun exposure, space requirements and weather are all factors that influence how and when you choose to prune. We're providing a starting place for you, and over time you will learn the particularities of this plant in your garden. Don't be afraid to get started – Salvias, in general, are quick to rebound if inappropriately pruned.
Deadheading – the removal of spent flowers, is a practice that will always benefit the plant's health and appearance. This can be done at any time. Pruning involves removal of entire stems of spent growth. Becoming "spent" means that flowering stems stop blooming and begin going to seed.
During the spring and summer, you can completely cut to the ground any stems that have finished blooming and are becoming unsightly.
In mild climate areas, growth can be so rapid that the entire plant becomes messy and spent mid-way through the season. In this case, it can be cut back close to the ground – given a short "haircut". The result usually is fresh, vigorous new growth and another round of flowering.
(Eyelash Sage) Small, eyelash-like hairs on the edge of its leaves give this Mexican native part of its name. A compact, gently mounding Salvia, it spreads gradually by underground stolons.
'Old Form' was collected in the wild and is a vigorous variety. All clones of this species vary in color depending on the light in which they grow as well as variations in ambient temperature and time of year. It is, at times, a rich reddish-orange as the photo indicates. However, instead of trying to describe all its color variations and compare them to the flowers of the other Eyelash Sages we offer --'Painted Lady' and Diablo' -- we suggest that you try all three!
(Painted Lady Eyelash Sage) Small, eyelash-like hairs on the edge of its leaves give this Mexican native part of its name. A compact, gently mounding Salvia, it spreads gradually by underground stolons.
Similar to Diablo Eyelash Sage, the richly colored flowers of this variety are darker in full sun and paler in partial shade. It's red-orange flowers are brighter than those of "Diablo' and often cover the plant in large clusters from early summer to late fall. Enjoy it in a mixed border.
(Red Michoacán Sage) No other Salvia has flowers that are such a deep blood red. The 3-to-4 inch long tubular blossoms of this shade-loving shrub are displayed in clusters at the ends of the stems, which have light green, textured leaves that are almost round.
This sage is a beauty in containers and shrubby borders or as a groundcover. Although it can tolerate full sun, Red Michoacán prefers partial shade. So, if necessary, compromise by choosing a location with morning sun and afternoon shade. Sun or shade, hummingbirds will find their way to its nectar. Deer most likely will munch elsewhere.
Confusion surrounds the scientific name of this plant. At various times, It has been improperly identified as Salvia tubiflora and as Salvia tubifera, which both are orange-flowered, Peruvian species. However, the foliage and growth habit of Salvia longistyla is much different. This is the true, fall-blooming species.
(Orange Mountain Sage) Coahuila, Mexico, is home to many fine Salvias, including the smallest variety of Salvia regla that we grow. This one averages about 3 feet tall and wide.
This fragrant, compact Salvia regla has tidy foliage and large, orange flowers that bloom from summer into fall in USDA Zones 7 to 10. The absolutely unique characteristic of this variety is its bright orange bracts that even turn the heads of longtime Salvia enthusiasts.
A native of the Chisos Mountains in Southwestern Texas and of Mexico from Coahuila to Oaxaca, Salvia regla is powerfully heat tolerant and fragrant. Although it appreciates average watering based on local conditions, the species does well in waterwise gardens. Give it full sun and well-drained soil. Grow it as a screen, shrub border or background plant. This is a favorite in native gardens and dry gardens.
Hummingbirds love this species, which has become an important nectar source for their southbound, autumn migration to the tropics. Butterflies also visit. So it's almost impossible to keep this plant in stock when in bloom.
NOTE: Theses are slow to propagate, and generally take at least 8 weeks for an appropriate plant to be grown.
(Venezuelan Red Sage) Purple stems and calyxes so dark that they almost look black contrast dramatically with the deep red-orange flowers of this South American beauty. This tall, spectacular sage has been in cultivation for decades but is still rare in gardens. We'd like to see that change.
The heavily textured leaves are large, growing about 4 inches long and 3 inches wide. They are hairy, which gives the foliage a silvery appearance. The flower spikes grow up to 2 feet tall.
This is a robust shrub that requires full sun to partial shade, average watering and rich, well-drained soil. Plant it in a prominent place so you can enjoy its long bloom throughout fall as well as the hummingbirds who use it regularly. At our farm its flowering synchronizes with the Dahlias. Garden uses include shrub borders, cut-flower gardens, background plantings and patio containers.
Rubescens refers to the reddish color of the flowers. This native of Venezuela was first described by Reinhard Gustav Paul Kunth in 1817.
Although we list this species for USDA Cold Hardiness Zones 9 to 11, we believe it may be hardy to Zone 8 winters if treated as an herbaceous perennial and well mulched.
Highly recommended.
(John Whittlesey Sage) Hardy, vigorous and long blooming, John Whittlesey Sage is a hybrid of D'Arcy's Sage (Salvia darcyi) -- a native of Mexico -- and Mountain Sage (S. microphylla), which is native to the American Southwest and Mexico.
The long flowering season of this sage makes John Whittlesey Sage a garden favorite; it begins bursting with salmon-red blooms early in the growing season. You can grow it as a bedding plant in areas with winters cooler than those of USDA Zone 7. In warmer zones, this tidy sage is an herbaceous perennial.
In coastal areas, John Whittlesey Sage is a great stand-in for the plethora of little-leaf species -- Mountain Sage, Autumn Sage (S. greggii )and Jame Sage (S. x jamensis) -- that often struggle with humidity.
Hummingbirds love the bright red flowers of this full-sun, heat-tolerant plant that makes a tall but effective groundcover. However, it is generally used in mixed borders.
Horticulturist Mike Thiede of Chico, California, developed this sage and named it for John Whittlesey of Canyon Creek Nursery in Oroville, California.
(Tangerine Pineapple Sage) This citrus-scented cultivar is our smallest variety of Pineapple Sage. Worth growing just for the exotic scent of its leaves, this culinary Salvia is also one of the longest blooming plants in its species.
How is this variety of Pineapple Sage different from Honey Melon? Tangerine's leaves are much smaller (1/2 inch x 1 inch as opposed to 1 inch x 1 1/2 inches), and the plant is shorter (18 inches tall vs. 24 inches). Tangerine also has darker red flowers, foliage with a very different scent and a shrubbier look. Of course, anyone who loves scented plants should have both.
Tangerine Pineapple Sage spreads into a dense clump with underground runners. By cutting back older stems to the ground, new fresh growth keeps it in flower for months. On the Northern California coast, it starts blooming no later than May and sometimes continues until February.
Grow this cultivar in partial shade in warmer zones or in full sun in the coolest part of its range. Along with Honey Melon, Tangerine is easier to grow in most of the country than the larger-growing varieties of Pineapple Sage.
Native to Mexico, Pineapple Sage is found at high elevations in Pine and Oak forests. The species is used as a medicinal herb -- such as in herb tea -- to relieve anxiety and treat hypertension. Just smelling the leaves makes us happier.
Hummingbirds love Salvia (sage) nectar and are attracted to it by the bright colors of tubular sage blossoms. In particular, these little whirlybirds can easily spot flowers in the red spectrum, which is prevalent among sages. Here are some hummingbird gardening tips.
If you live in suburbs or rural areas where deer plunder gardens, Salvias (sages) can be part of your plan for discouraging these hungry visitors. Here are some tips.


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Adding Comments
Much as a garden grows, we hope the seeds planted with this issue will bear fruit and deepen your experience and understanding of pre and perinatal psychology and health.
We begin with a wonderful article from our founder, Thomas Verny, M.D. Dr. Verny's discussion of the pre and perinatal origins of childhood and adult diseases and personality disorders brings you new and comprehensive research establishing those origins. We welcome Dr. Verny back to these pages after much too long an absence and hope he will continue to bring us his insights and knowledge in future articles.
Dr. Tony Madrid, Arauna Morgan, Alanna Taormina, Lorena Laforest, and Elizabeth West present us with eight case studies in support of their approach to bonding therapy, which they call the "fourpart repair." The promise of this approach to healing ruptured bonding is profound. If healing can be accomplished through the mothers, their children will enter into parenthood without the burden of unhealed bonding injuries. Many generations may benefit from this work.
Dr. Michel Odent's exposition on the Waterside hypotheses invites us to consider the obstetrical implications of this alternative look at evolution and begins to make important connections for our consideration. This article continues Dr. Odent's tradition of inviting us to think in new ways and envision new possibilities.
In the book review section, we bring you an outstanding review by William Emerson, APPPAH's esteemed president, of Marcy Axness' long-awaited, extremely well researched treatise on parenting for peace.
One final note, we invite you to become an active part of APPPAH through contributions to this journal. Please contact me at email@example.com for a copy of the author guidelines and prepare to join your esteemed colleagues on these pages.
JOURNAL OF PRENATAL AND PERINATAL PSYCHOLOGY AND HEALTH publishes research and clinical articles from the cutting edge of the science of prenatal and perinatal psychology and health. The journal, published quarterly since 1986, is dedicated to the in-depth exploration human reproduction and pregnancy and the mental and emotional development of the unborn and newborn child.

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Mr. LEAHY. Mr. President, originally we were planning to vote at 5:30 p.m. The distinguished ranking member has no objection. I ask unanimous consent that the time be divided between now and 5:30 p.m. in the normal fashion and the votes be at 5:30 p.m.
The PRESIDING OFFICER. Is there objection?
Without objection, it is so ordered.
Mr. LEAHY. Mr. President, so Senators will know, it is my understanding that the first vote, on the confirmation of Fernando Olguin, of California, will be a voice vote, and the second one for Mr. Durkin will be a rollcall vote, which is what I understand from the Senator from Iowa, which, of course, is perfectly acceptable to the Democratic side.
Mr. LEAHY. Mr. President, today the Senate is finally being allowed to vote to confirm two consensus judicial nominees who should have been voted on months ago. Both Judge Fernando Olguin and Thomas Durkin were voted out of the Judiciary Committee before the August recess. Both will finally fill judicial emergency vacancies in the Central District of California and the Northern District of Illinois that were needlessly held vacant since this summer by partisan delay tactics. Their service to the American people has been unnecessarily delayed by over four months.
In the Central District of California, there are over 12,000 cases pending before its judges, and in the Northern District of Illinois there are close to 11,000 cases pending before its judges. Every single judge in each district has approximately 450 or more cases pending on their dockets. This enormous backlog of cases exists in many of our Federal courts in this country and it means that the American people are not able to receive speedy justice.
More than twice the number of judicial vacancies exists compared to the vacancies left at the end of President Bush's first term. The Senate should be voting on all 16 of the judicial nominees reported to the Senate by the Judiciary Committee. I have also been urging Republicans to expedite consideration of the four judicial nominees who participated in hearings last Wednesday. That would lead to 20 more confirmations before the Senate adjourns later this month.
Historically, the Senate has confirmed hundreds of judicial nominees within 14 days of their Judiciary Committee hearings, including more than 600 confirmed since World War II within just one week of their hearings. In contrast, obstruction by Senate Republicans has caused President Obama's district court nominees to wait an average of 103 days for a Senate vote after being reported by the Judiciary Committee, which Committee consideration has itself often been delayed 30 days or more after their hearings. This destructive practice of delaying for no good reason should be abandoned.
Republican filibusters and pocket filibusters are also preventing votes on circuit court nominees who should be confirmed by consensus before the Senate adjourns for the year. For example, one of the nominations Senate Republicans are holding up is that of Judge Robert Bacharach of Oklahoma to the Tenth Circuit, who they filibustered earlier this year. Senator Coburn, one of his home state Senators, said: ``He has no opposition in the Senate. . . . There's no reason why he shouldn't be confirmed.'' That also applies to Richard Taranto, who was reported more than eight months ago to a vacancy on the Federal Circuit. That applies to William Kayatta of Maine, who was reported nearly eight months ago and has the support of his two home state Republican Senators.
After today's votes, there will still be nine judicial nominees stalled on the Senate Executive Calendar who were reported before the August recess, and who should have been confirmed months ago. Most are consensus nominees. All have the support of both their home state Senators, including their home state Republican Senators. The Senate should be voting to confirm all these nominees before the Senate adjourns for the year.
When George W. Bush was President, Senate Democrats cooperated in moving judicial nominees quickly through the Committee and to a confirmation vote at the end of the year. I did so whether I was Chairman or the ranking member. By way of example, in 2008 we confirmed five of President Bush's nominees just three days after their hearing. We have often been able to do this at the end of a Congress, and this year should be no exception especially given the high level of judicial vacancies plaguing our Federal courts.
Judge Fernando Olguin is nominated to fill a judicial emergency vacancy on the U.S. District Court for the Central District of California, where he has been serving as a Magistrate Judge for over a decade. He was the first Latino-American to serve as a Magistrate Judge in that District. Prior to that, Judge Olguin was in private practice for several years and also served as a Trial Attorney at the Civil Rights Division of the U.S. Department of Justice. He earned his law degree from the University of California at Berkley. After law school, he clerked for the Honorable C.A. Muecke of the U.S. District Court for the District of Arizona. His nomination has the support of both his home state Senators. Judge Olguin was approved by the Judiciary Committee nearly five months ago by voice vote.
Thomas Durkin is nominated to fill a judicial emergency vacancy on the U.S. District Court for the Northern District of Illinois. Currently a partner at the Chicago office of Mayer Brown LLP, he also served as a Federal prosecutor in the Northern District of Illinois for 13 years. During his time as a Federal prosecutor, he rose to become the Chief of the Criminal Receiving and Appellate Division as well as the Chief of the Special Prosecutions Division. From 1991 to 1993, he served as the First Assistant United States Attorney of that District. Upon graduation from law school, he served as a law clerk for the Honorable Stanley J. Roszkowski of the U.S. District Court for the Northern District of Illinois. The ABA Standing Committee on the Federal Judiciary unanimously gave him its highest possible rating of ``Well Qualified.'' His nomination has the bipartisan support of his home state Senators. He was approved by the Judiciary Committee more than four months ago by voice vote.
The Senate should finally confirm these two nominees today and proceed to vote on all the other judicial nominees stalled on the Senate Executive Calendar. We can fill 10 more judicial emergency vacancies before adjourning this year. We can help our Federal courts uphold their constitutional responsibility to provide speedy justice.
I yield the floor.
Source: http://thomas.loc.gov


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While we work on our update for iOS 7. PLEASE download our new product eKneeBoard. create a free user account and get the chart subscription for the ...
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While we work on our update for iOS 7. PLEASE download our new product eKneeBoard. create a free user account and get the chart subscription for the state of your choice for free, for life time. (no US pick a state you may train in) Checkout eKneeBoard, in the App Store, for the BEST iEFB with Integrated Interactive Training Courses and a National VFR/IFR GeoReferenced Yearly Data Plan for UNDER 25 bucks. Our AIRPLANE MULTI-ENGINE GROUND SCHOOL course will prepare you to become a multi engine pilot. Whether you are adding a rating or applying for a new pilot certificate level this course will give you all you need to know about flying a twin in normal and emergency situations covering both academic content and flight maneuvers videos. You'll enjoy revealing animations of the airplane systems and multi engine aerodynamics and you'll see all the maneuvers, takeoffs, landings, go arounds and emergency procedures demonstrated for you in a special three view display that lets you see an outside view, instrument view and chase plane view for all private and commercial multi engine maneuvers. TRY before you buy: There are several free lessons included in this download. Unlock the full content with a simple in-app purchase, or through a participating flight school, or on our website. The complete course includes over 3 hours of solid training on the following topics: Introduction to flying multi engine airplanes General considerations Principles of flight with one engine inoperative Understanding Vmc Fuel system Electrical system Environmental systems Landing gear system Engine and propeller systems Aircraft controls, trim and braking systems Understanding Performance charts and calculations Weight and balance Multi engine procedures - Maneuvers at altitude Multi engine procedures - Takeoffs, landings and go arounds Multi engine procedures - Emergency procedures This course can be used with any multi engine training syllabus at either the Private or Commercial pilot levels; note however that the aircraft systems and performance sections are based upon the Piper Seminole. Each lesson contains a quiz at the end to test your knowledge of what you have learned. The Multi engine procedures let you watch the maneuvers and procedures performed as they are supposed to be and are completely narrated with annotations of important things to remember and do. All content is resident on your iPad/iPhone. You don't need an Internet connection to view the lessons, take the quizzes, or read the handbooks. This is one or many aviation education programs offered by AnywhereEducation and MS Aviation (MS Aviation is now "AnywhereEducation Aviation Group") * Over 330,000 users of our aviation series courses. * Several Major US Universities use our courseware in their Aeronautics Programs. * Many large flight schools use our courseware in their Career Pilot Training Programs. * Dozens of small to mid sized flight schools use our courseware. * Ask your flight school today to join our approved schools program. Our email support is available 24 hours x 7 days a week. Most support questions are resolved within 4 hours. email Support at: firstname.lastname@example.com
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The Port of Dover's leading project, the Dover Western Docks Revival (DWDR), is quite literally laying the foundations for future opportunities as reclaimed concrete from local building works form the foundation of the Western Docks.
Over the past 10 months 46,000 tonnes of reclaimed concrete (aggregate) from the Prince of Wales Pier enabling works and demolition material from the St James development has been stockpiled for the DWDR project, creating a sustainable legacy of material processing and recycling.
Jack Goodhew, General Manager – Special Projects of the Port of Dover, said: "We make every effort to send materials from old structures for recycling, rather than to landfill.
"The DWDR project is delivering sustainable development by reducing the volume of aggregate required and we have been obtaining suitable material from local demolition works in order to minimise our requirements from other sources.
"The height reduction and enabling works along the Prince of Wales Pier have resulted in 20,000 tonnes of recycled concrete crush added to the 26,000 tonnes already recycled from the demolition of the former 1970's office block, hotel and multi-storey car park from Dover District Council's development.
"This resulted in a reduction of 46,000 tonnes of embodied carbon associated with the extraction, manufacture and delivery of aggregate.
"The DWDR project will make best use of recycled materials wherever possible from local regeneration projects, and this also includes a further 7,000 tonnes from the multi-storey car park in the Eastern Docks which is due to be demolished next year."
Search and find the perfect commercial property in Kent for your business. Locate in Kent gives advice and relocation support to international and UK companies looking to move or expand. Call +44 (0)1732 520700 or email email@example.com
Locate in Kent would like to thank - Canterbury Christ Church University, Discovery Park, Frogmore, Kent County Council, Liberty Property Trust UK, London Array, Medway Council, Thanet Earth, University of Kent and Visit Kent - for the use of their images on this website.


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by Staff Writers
Beijing (AFP) Jan 3, 2016
Beijing has rejected a protest from Vietnam after a Chinese plane landed on a contested reef in the South China Sea, saying the operation took place within Chinese territory.
A Chinese "test flight" landed on Fiery Cross reef, foreign ministry spokeswoman Hua Chunying said in an online statement late Saturday. Vietnam also claims the reef.
China has asserted its claim to almost all of the South China Sea by rapidly building artificial islands including airstrips said to be capable of hosting military jets.
It began work in 2014 on a 3,000-metre (9,842 feet) runway on Fiery Cross reef in the Spratlys island group, around 1,000 kilometres (620 miles) from China's island province of Hainan.
Hua said the test flight was civilian in nature, adding that the "relevant activity falls completely within China's sovereignty".
Hanoi earlier strongly protested at the flight, labelling it a violation of sovereignty which "influences peace and stability in the South China Sea".
"Vietnam resolutely protests China's above-mentioned action, asking China to immediately end while not repeating similar move," said foreign ministry spokesman Le Hai Binh in Hanoi.
Vietnamese officials also said they had asked Beijing to investigate the ramming and sinking of a Vietnamese fishing boat by a suspected Chinese boat.
The fishermen were around 60 nautical miles from Con Co Island in central Quang Tri province on Friday when a foreign boat crashed into their craft.
The 11 crew members were rescued but the boat sank, the fishermen told the VNExpress news site.
The captain was quoted as saying that he saw Chinese characters on the foreign boat.
Ha Le, deputy head of the Vietnam Fisheries Surveillance Department, told AFP Chinese officials had offered to check on the report if more details became available.
Relations between the communist neighbours have grown tense in recent years over the disputed Spratly and Paracel island chains.
Rioting broke out in Vietnam after Beijing sent an oil rig into contested waters in 2014, and at least three Chinese people were killed.
Since then the two sides have tried to mend relations. China's President Xi Jinping visited Hanoi in November but that visit also saw anti-Chinese protests.
Hanoi has stepped up cooperation with the US, in what analysts say is a hedge against China's rising power.
Several other claimants have also built facilities in the South China Sea but at a slower pace than China.
The Philippines, Malaysia, Brunei and Taiwan also have claims to parts of the Sea, home to strategic shipping lanes as well as substantial oil and gas reserves.
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Connect with Shane's DJ Service in Myrtle Beach, SC
Shane's DJ Service Tags on Party Blast: Myrtle Beach Area DJ with the knowledge to entertain at an event. My music library contains over 90,000 songs and is updated weekly. Karaoke is also available. DJ /Dancing lights are provided. wedding dj, Bose Premium Sound


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Fletcher Technical Community College basketball news
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Get the facts about Fletcher Technical Community College basketball news, and registering for discussion sections and departmental seminars. Not all recruiting is done like NCAA Division I teams. College coaches want to hear from serious recruits throughout their high school career. Don't be shy to send videos of your best games to college coaching staff or scouts. Finally, check with your major department for assistance with prerequisites, tuition payment deadlines, and Fletcher Technical Community College Basketball information. Please use the form above to request admissions info for Fletcher Technical Community College.
You don't have to quarterback the university football team to a state championship to earn an athletic scholarship. Most major sports that field a team in intercollegiate competition have sports scholarships available. From women's gymnastics to men's track and field, coaches are seeking players to build a team around. Further, that leadership role often is accompanied by a full-scholarship.
The best time to begin searching for scholarships is before your senior year, in order to meet deadlines. Schools confirm that it is important to file financial aid forms such as the CSS Profile early in the school year. There are many sports scholarships that focus on less tangible qualities, such as the ability to lead a team, motivate others, and learn new skills rapidly. Take stock of your athletic abilities as you mature through high school. Cal State San Marcos baseball players drafted may encourage student-athletes to apply via early decision. Students who are considering applying via early decision should be aware of the rules and obligations that this commitment entails. Beyond consideration for Fletcher Technical Community College basketball news, you may think about National Louis University research papers for complementary electives to your primary course of study.
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Results 1 to 11 of 11
May 2nd, 2005, 08:28 PM #1
My 1000th Post - Thanks Everyone!
- Join Date
- January 18th, 2005
- Location
- Small Town in Tennessee
- Posts
- 5,226
There is no way I can thank the folks here for all they've done.
With your help, I have learned BIG things - that have vastly improved my sites (and pocketbook)
And zillions of little things - that combined - improved my sites and pocketbook.
If you look at any of my sites pre-ABW, and compare them to post-ABW, they are in a completely different league!
There's a little piece of you in all my sites.
The commoraderie here is great. Nice to know we're not alone.
The fact that I can come here and vent keeps me off illicit drugs or jumping of the Brooklyn Bridge when dealing with... well... we all know what we have to deal with
I'd thank all the people I learned from individually, but there is literally over 100 of you! Probably more.
I only hope I've given back 1/2 as much as I received.
And to the merchants who hang here:
Thanks for all your help, dedication, ideas, and support. You've proved beyond any doubt that if a program is to succeed, it needs a pro-active, available, supportive AM.
Again, I couldn't have done it without you "nameless horde of professionals"
Lastly, thanks to "H" for this valuable resource.
There! That was my PROFESSIONAL 1000th post!
If you need me, I'll be in the Linkshare forum doing my usual post!
May 2nd, 2005, 08:51 PM #2
- Join Date
- January 18th, 2005
- Location
- The Swamp
- Posts
- 7,503
Congrats on the 1000th post Billy! And thank YOU for being a part of this community.
May 2nd, 2005, 08:59 PM #3or jumping of the Brooklyn Bridge
Why travel all the way to NY to do that?
The Golden Gate Bridge is closer to Vegas and on July 31, 2005 the Golden Gate Bridge roadway will once again be part of the San Francisco Marathon.
Go run the marathon first.
May 2nd, 2005, 09:10 PM #4
1000th post ??? Who is this Billy Kay? Is it male or female?
Have I been fortunate enough to not read any of his posts? LOL
Well done Billy. I bet its not long before your back here posting "My 2000th Post - Thanks Everyone!"One day parasites and their ilk will be made illegal, I bet a few Lawyers will be pissed off when the day comes.
Mr. Spitzer is fetching it nearer
May 2nd, 2005, 10:12 PM #5
My Friend and valued member alllow me to thank you for your participation and dedication in the truth in affiliate marketing via ABW for you and our other members.
Congrats on the 1K posts ... I guess I gotta send you a BWare Pware Tshirt now ... but ... I think I might have do something "extra" special on the back of it (pennies from LS wise) make sure to PM me your size and we'll make your special limited edition shirt.
Continued Success and Postings,Continued Success,
The secret of success is constancy of purpose ~ Disraeli
May 3rd, 2005, 01:26 AM #6
- Join Date
- January 18th, 2005
- Location
- St Clair Shores MI.
- Posts
- 17,328
Congrats Billy Kay on hanging in there. Are you related to Mary Kay? If so request a pink Pware T-shirt like the one Gordo got by padding a 3000 pageview thread. You must be a Haiko groupee with some LS pull getting him to fawn over you like that. I strive to achieve stature here like an Howard Stern type icon.... but end up a bad imitation of Rodney Dangerfield. I do enjoy your posts and don't forget to put some bait on those hooks.Webmaster's... Mike and Charlie
"What have you done today to put real value into a referral click...from a shoppers viewpoint!"
May 3rd, 2005, 01:55 AM #7
May 3rd, 2005, 09:19 AM #8
Congrats Billy for contributing to the community and for the affiliate marketing industry in general.....
Let's see you get to the 5K mark by this time next year....Andy Rodriguez Consulting, Affiliate Program Management and Consulting Services, Since 2001
www.andyrodriguez.com | E: firstname.lastname@example.com | P: (888) 931-ANDY (2639) | Skype: affiliatedoctor | AIM & MSN: AffiliateDoctor | Subscribe To Our ABW Forum Posts | Follow me on Twitter | Join Our Affiliate Programs
May 3rd, 2005, 10:41 AM #9
I do more reading than posting..
I keep hearing that small chineese man (confusios, I think) telling me to keep my mouth shut and be thought a fool....Marty Ogelvie
Dallas Cowboys fan | New York Yankees fan
May 5th, 2005, 07:57 PM #10
Congrats Billy
You have helped me a few times without knowing
Btw I will be out your neck of the woods next week 5/8 to 5/14 Hopefully I will be richVinny O'Hare - OPM - Contact Info email: vinny at teamloxly.com ~ 702-582-6742 Twitter
May 5th, 2005, 11:08 PM #11
- Join Date
- January 18th, 2005
- Location
- Small Town in Tennessee
- Posts
- 5,226
I went to NYC for St patrick's day.
MY calendar said it was spring. Looking out my window said it was spring.
It was friggin' snowin! There was a sign on the window of my room at the Hyatt "Don't open the window. Pipes will freeze and burst. Cause damage"
And since I smoke, I had to go stand in the snow to light up!
Ahhh. welcome home!!
Bring a pretzel! Your comin BEFORE the convention?
I'll PM my cell.
PS to AndyR - thanks for havin' thick skin!
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- We will support our own members going to the field as a first priority.
- We will make sure our own current missionaries /partnerships financial needs are met prior to adding new missionaries/partnerships to support.
- Recognizing that nationals sharing the gospel within their own people group have a distinct advantage, we will give priority to the support of national missionaries prior to giving to expatriate missionaries.
- We will give priority to support missionary activity to people groups where there is the greatest population and the least amount of missionary activity.
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Knowing care for the environment is one of our most important missions.
These are our ECOprácticas that make us be the first hotel in Easter Island working in a friendly and environmentally sustainable.
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Uses less water
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Saving energy
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News from the Bench
Discover what's going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.
MeDIP Kits and Reagents
Immunoenrich for regions of the genome with modified nucleotides
To Purchase # 74178S
|100 µl (10 Dot Blots)
Find answers on our FAQs page.
PhosphoSitePlus® Resource
- Additional protein information
- Analytical tools
5-Formylcytosine (5-fC) (D5D4K) Rabbit mAb #74178
Confocal immunofluorescent analysis of 293T cells transfected with a construct expressing DDK-tagged TET1 catalytic domain (TET1-CD) using 5-Formylcytosine (5-fC) (D5D4K) Rabbit mAb (green) and DYKDDDDK Tag (9A3) Mouse mAb #8146 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). As expected, 293T cells expressing TET1-CD (red) exhibit increased levels of 5-formylcytosine (green).Learn more about how we get our images
The specificity of 5-Formylcytosine (5-fC) (D5D4K) Rabbit mAb was determined by dot blot. The same sequence of a 387-base pair DNA fragment was generated by PCR using exclusively unmodified cytosine, 5-methylcytosine (5-mC), 5-hydroxymethylcytosine (5-hmC), 5-carboxylcytosine (5-caC), or 5-formylcytosine (5-fC). The respective DNA fragments were blotted onto a nylon membrane, UV cross-linked, and probed with 5-Formylcytosine (5-fC) (D5D4K) Rabbit mAb. The upper panel shows the antibody only binding to the DNA fragment containing 5-fC, while the lower panel shows the membrane stained with methylene blue.Learn more about how we get our images
5-Formylcytosine (5-fC) (D5D4K) Rabbit mAb specificity was determined by ELISA. The antibody was titrated against a single-stranded DNA oligo containing either unmodified cytosine or differentially modified cytosine (5-mC, 5-hmC, 5-caC, 5-fC). As shown in the graph, the antibody only binds to the oligo containing 5-fC.Learn more about how we get our images
Gallery: 5-Formylcytosine (5-fC) (D5D4K) Rabbit mAb #74178
Immunofluorescence (Immunocytochemistry)
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
Stock Solutions
- 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix. Adjust pH to 8.0.
- Ethanol, 70% solution, deionized.
- 1.5 M Hydrochloric acid.
Blocking Buffer (1X PBS / 5% normal serum / 0.3% Triton™ X-100):
To prepare 10 ml, add 0.5 ml normal serum from the same species as the secondary antibody (e.g., Normal Goat Serum (#5425)) and 0.5 mL 20X PBS to 9.0 mL dH2O, mix well. While stirring, add 30 µl Triton™ X-100.
- Antibody Dilution Buffer (1X PBS / 1% BSA / 0.3% Triton™ X-100): To prepare 10 ml, add 30 µl Triton™ X-100 and 0.5 mL 20X PBS to 9.5 mL dH2O. Mix well then add 0.1 g BSA (#9998), mix.
Recommended Fluorochrome-conjugated Anti-Rabbit secondary antibodies:
- Prolong® Gold AntiFade Reagent (#9071), Prolong® Gold AntiFade Reagent with DAPI (#8961).
B. Specimen Preparation - Cultured Cell Lines (IF-IC)
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
- Aspirate media, cover cells completely with ice-cold 70% ethanol.
- Allow cells to fix for 5 minutes at room temperature.
- Aspirate fixative, rinse three times in 1X PBS for 5 minutes each.
- Add 1.5 M HCl and incubate for 30 minutes at room temperature.
- Aspirate HCl and rinse two times in 1X PBS for 5 minutes each.
- Proceed with Immunostaining section C.
C. Immunostaining
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
- Block specimen in Blocking Buffer for 60 minutes.
- While blocking, prepare primary antibody by diluting as indicated on datasheet in Antibody Dilution Buffer.
- Aspirate blocking solution, apply diluted primary antibody.
- Incubate overnight at 4°C.
- Rinse three times in 1X PBS for 5 minutes each.
- Incubate specimen in fluorochrome-conjugated secondary antibody diluted in Antibody Dilution Buffer for 1–2 hours at room temperature in dark.
- Rinse three times in 1X PBS for 5 minutes each.
- Mount samples in an appropriate antifade reagent such as Prolong® Gold Antifade Reagent (#9071) or Prolong® Gold AntiFade Reagent with DAPI (#8961).
- For best results, allow mountant to cure overnight at room temperature. For long-term storage, store slides flat at 4°C protected from light.
posted December 2015
DNA Dot Blot Protocol
A. Buffers and Reagents
- 20X Saline Sodium Citrate (SSC) Buffer: 3.0 M NaCl, 0.3 M Sodium Citrate, pH to 7.0.
- 10X SSC Buffer: Dilute 20X SSC buffer 1:2.
- 2X DNA Denaturing Buffer: 200 mM NaOH, 20 mM EDTA.
- Nuclease-Free Water: (#12931)
- Blotting Membrane: This protocol has been optimized for positively charged nylon membranes.
- 96-Well Dot Blot Apparatus
- 10X Tris Buffered Saline with Tween® 20 (TBST): (#9997) To prepare 1 L 1X TBST: add 100 ml 10X TBST to 900 ml dH2 0, mix.
- Nonfat Dry Milk: (#9999)
- Blocking Buffer: 1x TBST with 5% w/v nonfat dry milk; for 150 ml, add 7.5 g nonfat dry milk to 150 ml 1X TBST and mix well.
- Bovine Serum Albumin (BSA): (#9998)
- Primary Antibody Dilution Buffer: 1X TBST with 5% BSA; for 20 ml, add 1.0 g BSA to 20 ml 1X TBST and mix well.
- Secondary Antibody Conjugated to HRP: anti-rabbit (#7074); anti-mouse (#7076).
- Detection Reagent LumiGLO® chemiluminescent reagent and peroxide (#7003) or SignalFire™ ECL Reagent (#6883)
B. Dot Blot
Note: This protocol is written for spotting fragmented, purified genomic DNA (titration of 1000 ng, 500 ng, 250 ng, 125 ng, 62.5 ng, 31.25 ng, and 15.625 ng) onto a positively charged nylon membrane using a 96-well dot blotting apparatus. Depending on the source and type of DNA, more or less DNA may be required for detection with the antibody.
Before Starting:
• Purify genomic DNA using a genomic DNA purification protocol or kit and sonicate
genomic DNA to generate fragments between 200 and 500 bp. DNA fragment size
can be analyzed by gel electrophoresis on a 1% agarose gel with a 100 bp DNA
• Cut a piece of nylon membrane to the size of the dot blot manifold.
• Wet nylon membrane with 10x SSC Buffer.
• Dry membrane by placing it in 96-well dot blot apparatus and applying vacuum.
Dilute fragmented genomic DNA to 100 ng/μl in 100 ul of nuclease-free water.
Then denature DNA by adding 100 μl of 2X DNA Denaturing Buffer and incubating at 95°C for 10 min.
- Add 200 μl of 20X SSC buffer and immediately chill on ice for 5 min.
- Add 100 μl of nuclease-free water to bring DNA solution to a final volume of 500 μl with a DNA concentration of 20 ng/μl.
- Set up a series of six 2-fold dilutions by adding 250 μl of the DNA solution, starting with the DNA solution in Step 3, to 250 μl of nuclease-free water. This will generate seven DNA samples containing 250 μl DNA at concentrations of 20 ng/μl, 10 ng/μl, 5 ng/μl, 2.5 ng/μl, 1.25 ng/μl, 0.625 ng/μl, and 0.3125 ng/μl.
- Apply 50 μl of each of the seven dilution samples into separate wells of the 96-well dot blot apparatus, leaving the last well for nuclease-free water only. The amount of DNA added to each well should then be 1000 ng, 500 ng, 250 ng, 125 ng, 62.5 ng, 31.25 ng, 15.625 ng and 0 ng respectively. Apply gentle vacuum pressure to draw solution through the membrane. Nylon membrane should be mostly dry before step 6.
- Remove nylon membrane from the 96-well dot blot apparatus and wrap in plastic wrap.
- UV cross-link nylon membrane at 1200 J/m2.
C. Membrane Blocking and Antibody Incubation
- Incubate membrane in 25 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
- Wash membrane three times for 5 min each with 15 ml of 1X TBST.
- Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the antibody product datasheet) in 10 ml primary antibody dilution buffer, with gentle agitation overnight at 4°C.
- Wash three times for 5 min each with 15 ml of 1X TBST.
- Incubate membrane with the species appropriate HRP-conjugated secondary antibody (#7074 Anti-rabbit IgG, HRP-linked Antibody or #7076 Anti-mouse IgG, HRP-linked Antibody) at 1:2000 in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
- Wash membrane three times for 5 min each with 15 ml of 1X TBST.
- Proceed with detection (Section D)
D. Detection of DNA
- Incubate membrane with 10 mL of LumiGLO® (0.5 ml 20x LumiGLO® #7003, 0.5 ml 20x Peroxide, and 9.0 ml purified water) or 10 ml SignalFire™ #6883 (5 ml Reagent A, 5 ml Reagent B) with gentle agitation for 1 min at room temperature.
- Drain membrane of excess developing solution (do not let dry), wrap in plastic wrap and expose to x-ray film. An initial 10 sec exposure should indicate the proper exposure time.
NOTE: Due to the kinetics of the detection reaction, signal is most intense immediately following incubation and declines over the following 2 hr
posted November 2015
|Immunofluorescence (Immunocytochemistry)
|DNA Dot Blot
5-Formylcytosine (5-fC) (D5D4K) Rabbit mAb detects 5-fC by IF in cells over-expressing the TET1 catalytic domain and by dot blot using double-stranded PCR fragments containing 5-fC. Many cells and tissues contain very low endogenous levels of 5-fC that may fall below the detection limits of this antibody. This antibody has been validated for specificity using ELISA and dot blot and shows high specificity for 5-fC.Species Reactivity: All Species Expected
Monoclonal antibody is produced by immunizing animals with 5-formyl-2'-deoxycytosine.
Methylation of DNA at cytosine residues is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting, and mammalian development (1,2). 5-methylcytosine is a repressive epigenetic mark established de novo by two enzymes, DNMT3a and DNMT3b, and is maintained by DNMT1 (3, 4). 5-methylcytosine was originally thought to be passively depleted during DNA replication. However, subsequent studies have shown that Ten-Eleven Translocation (TET) proteins TET1, TET2, and TET3 can catalyze the oxidation of methylated cytosine to 5-hydroxymethylcytosine (5-hmC) (5). Additionally, TET proteins can further oxidize 5-hmC to form 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC), both of which are excised by thymine-DNA glycosylase (TDG), effectively linking cytosine oxidation to the base excision repair pathway and supporting active cytosine demethylation (6,7).
TET protein-mediated cytosine hydroxymethylation was initially demonstrated in mouse brain and embryonic stem cells (5, 8). Since then this modification has been discovered in many tissues, with the highest levels found in the brain (9). While 5-fC and 5-caC appear to be short-lived intermediate species, there is mounting evidence showing that 5-hmC is a distinct epigenetic mark with various unique functions (10,11). The modified base itself is stable in vivo and interacts with various readers including MeCP2 (11,12). The global level of 5-hmC increases during brain development, and 5-hmC is enriched at promoter regions and poised enhancers. Furthermore, there is an inverse correlation between levels of 5-hmC and histone H3K9 and H3K27 trimethylation, suggesting a role for 5-hmC in gene activation (12). Lower amounts of 5-hmC have been reported in various cancers including myeloid leukemia and melanoma (13,14).
1. Hermann, A. et al. (2004) Cell Mol Life Sci 61, 2571-87.
2. Turek-Plewa, J. and Jagodziński, P.P. (2005) Cell Mol Biol Lett 10, 631-47.
3. Okano, M. et al. (1999) Cell 99, 247-57.
4. Li, E. et al. (1992) Cell 69, 915-26.
5. Tahiliani, M. et al. (2009) Science 324, 930-5.
6. He, Y.F. et al. (2011) Science 333, 1303-7.
7. Ito, S. et al. (2011) Science 333, 1300-3.
8. Kriaucionis, S. and Heintz, N. (2009) Science 324, 929-30.
9. Globisch, D. et al. (2010) PLoS One 5, e15367.
10. Gao, Y. et al. (2013) Cell Stem Cell 12, 453-69.
11. Mellén, M. et al. (2012) Cell 151, 1417-30.
12. Wen, L. et al. (2014) Genome Biol 15, R49.
13. Delhommeau, F. et al. (2009) N Engl J Med 360, 2289-301.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. DRAQ5 is a registered trademark of Biostatus Limited.


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