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Contribution of translocon peptide channels to the permeation of low molecular mass anions was investigated in rat liver microsomes. Puromycin, which purges translocon pores of nascent polypeptides, creating additional empty pores, raised the microsomal uptake of radiolabeled UDP-glucuronic acid, while it did not increase the uptake of glucose-6-phosphate or glutathione. The role of translocon pores in the transport of small anions was also investigated by measuring the effect of puromycin on the activity of microsomal enzymes with intraluminal active sites. The mannose-6-phosphatase activity of glucose-6-phosphatase and the activity of UDP-glucuronosyltransferase were elevated upon addition of puromycin, but glucose-6-phosphatase and β-glucuronidase activities were not changed. The increase in enzyme activities was due to a better access of the substrates to the luminal compartment rather than to activation of the enzymes. Antibody against Sec61 translocon component decreased the activity of UDP-glucuronosyltransferase and antagonized the effect of puromycin. Similarly, the addition of the puromycin antagonist anisomycin or treatments of microsomes, resulting in the release of attached ribosomes, prevented the puromycin-dependent increase in the activity. Mannose-6-phosphatase and UDP-glucuronosyltransferase activities of smooth microsomal vesicles showed higher basal latencies that were not affected by puromycin. In conclusion, translationally inactive, ribosome-bound translocons allow small anions to cross the endoplasmic reticulum membrane. This pathway can contribute to the nonspecific substrate supply of enzymes with intraluminal active centers.
- puromycin
- UDP-glucuronosyltransferase
- glucose-6-phosphatase
- β-glucuronidase
several intraluminal enzymes of the endoplasmic reticulum (ER) gain their substrate supply from the cytosol. The substrates are often anions, which cannot cross the membrane by simple diffusion. Specific transporters may facilitate their movement across the membrane, although only a few ER transporters have been characterized at the molecular level (6, 20, 22). It has been repeatedly observed that microsomal vesicles derived from the ER exhibit a basal permeability toward various compounds, including xenogenous molecules, which presumably do not have strictly specific transporters. This nonspecific permeability is usually attributed to the damage or to the improper orientation of the membrane. However, recent observations indicate that translocon protein channels may play a role in the permeation of small molecules across the ER membrane.
Co-translational protein translocation and integration into the membrane of the ER occur at sites termed translocons (24, 28, 35–37). The translocon is composed of several ER membrane proteins that are associated to form an aqueous pore. This pore must maintain a permeability barrier because calcium ion is stored in the ER and the unregulated release of this potent second messenger would abolish a fundamental signaling mechanism of the cell. During co-translational translocation, the aqueous translocon pore is sealed at its cytoplasmic end by the growing polypeptide chain (35). The permeability of the translocon has been proposed to be regulated at its luminal side by the prominent ER chaperone BiP, a protein released from the translocon shortly after the completion of ribosome nascent chain targeting. In its empty state, when the translocon pore is ribosome-bound but unoccupied by polypeptide, the complex seems to allow the passage of small molecules (18, 33, 35).
Lomax et al. (27) studied the Ca2+ leak pathways in the ER of pancreatic acinar cells by directly measuring [Ca2+] in the ER. It was demonstrated that the leak was not blocked by either the IP3 receptor antagonist heparin or the ryanodine receptor antagonist ruthenium red, but they found a puromycin-induced Ca2+ efflux from the ER. Puromycin is an antibiotic that selectively terminates ribosomal translation by releasing the nascent polypeptide from the protein channel of the ribosome (32, 35, 38).
Wonderline and coworkers (18, 33) tested the permeation of a small neutral molecule, 4-methyl-umbelliferyl-α-d-glucopyranoside, by measuring its hydrolysis by an ER resident α-glucosidase, which is dependent on its entry into the ER. They found that translocons are permeable to this neutral molecule, as long as empty ribosomes remain bound to them.
It has been observed that low molecular weight anions, which presumably do not have specific ER transporters, e.g., mannose-6-phosphate, can cross the ER membrane, although with a moderate speed. Therefore, the aim of the present study was to investigate the permeation of small anions through translocon channels in rat liver microsomal vesicles. The transport was studied by two methods: 1) by comparing the activity of enzymes with intraluminal active sites in intact and permeabilized microsomes and 2) by directly examining the transport of substrates using the light scattering and rapid filtration techniques. The results confirmed that translocon channels can contribute to the permeation of small anions across the ER membrane.
Preparation of rat liver microsomes.
Liver microsomes were prepared from fed male Sprague-Dawley rats (180–230 g body wt; Charles River Hungary) as reported earlier (3). The livers were homogenized in sucrose-HEPES buffer (0.3 M sucrose and 0.02 M HEPES, pH 7.2) with a glass-Teflon homogenizer. The microsomal fraction was then isolated using fractional centrifugation. After centrifugation of liver homogenates for 10 min at 1,000 g, the supernatant was spun for 10 min at 12,000 g. The 12,000 g supernatant was centrifuged for 60 min at 100,000 g; the sediment was resuspended in buffer A containing (in mM) 100 KCl, 20 NaCl, 1 MgCl2, and 20 4-morpholinepropanesulfonic acid, pH 7.2, and was centrifuged again for 60 min at 100,000 g. The final pellet (microsomes) was resuspended in buffer A to give ∼50 mg/ml protein concentration, then immediately frozen in liquid nitrogen and kept in liquid nitrogen until use (within 2 mo). The microsomal fraction was characterized by measuring marker enzyme activities (5). The activity of cytochrome c oxidase, glucose-6-phosphatase, and 5′-nucleotidase in the microsomal fraction was 1.2 ± 0.3, 30.1 ± 5.0, and 6.0 ± 1.1, respectively, expressed as percents of activities measured in total homogenate (means ± SD, n = 3).
Rough and smooth subfractions of liver microsomes were prepared according to (5). The purity of the subfractions was evaluated by immunoblot analysis of Sec61α. Compared with the total microsomal fraction, 1.5-fold enrichment in the rough and fivefold impoverishment in the smooth subfraction was observed by the densitometry of the corresponding bands.
Integrity of microsomal vesicles was assessed by measuring the latency of mannose-6-phosphatase activity (8). Protein concentration of microsomes was determined using Bio-Rad protein assay with bovine serum albumin as a standard, according to the manufacturer's instructions.
Transport measurements by light-scattering technique.
The permeability of the microsomal membranes to sucrose was also measured by continuous detection of the osmotically induced changes in size and shape of microsomal vesicles (3, 25, 29). Briefly, ER vesicles (50 μg/ml protein) were equilibrated for 2 h in a hypotonic medium (5 mM 1,4-piperazine diethanesulfonic acid potassium salt, pH 7.0). Light scattering of the microsomal suspensions was then monitored at 400 nm with the use of a fluorimeter (Hitachi F-4500) equipped with a temperature-controlled cuvette holder (22°C) and magnetic stirrer. The addition of a small volume (<5% of the total incubation volume) of a concentrated osmolyte solution results in a rapid increase in light scattering (due to the shrinkage of the vesicles). This peak is followed by a gradual decrease in light scattering (which reflects vesicle swelling due to progressive equilibration of the osmolyte concentration between the extra- and intravesicular spaces). The relative height of the peak and the slope of the curve depend on the permeability of the vesicular membrane. Light-scattering registrations were terminated by the addition of the pore-forming compound alamethicin. The channels formed by alamethicin have been estimated to be 10 Å in diameter with no real selectivity between univalent ions. Alamethicin channels allow the passage of low-molecular-mass compounds such as dicarboxylates and nicotinamide nucleotides, but exclude folded proteins (Ref. 23 and references therein). Upon alamethicin addition, the signal returned to the basal level, indicating that the osmotic effects did not alter the vesicular structure of the microsomes.
Rapid filtration experiments.
For the measurement of the uptake and the accumulation of radiolabeled compounds, liver microsomes (1 mg protein/ml) were incubated in buffer A containing 0.02 mM UDP-glucuronic acid, sucrose, glucose-6-phosphate, or 0.4 mM glutathione plus their radiolabeled analogues (1 μCi/ml [14C]UDP-glucuronic acid, 1 μCi/ml [14C]sucrose, 10 μCi/ml [3H]glutathione, 2 μCi/ml d-[14C]glucose-6-phosphate) at 22°C. At the indicated time points, samples (0.1 ml) were rapidly filtered through cellulose acetate/nitrate filter membranes (pore size: 0.22 μm) and were washed with ice-cold buffer A containing 1 mM 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid. The radioactivity associated with microsomes retained by filters was measured by liquid scintillation counting. In each experiment, alamethicin (0.05 mg/mg protein) was added to parallel incubates to distinguish the intravesicular and the bound radioactivity (3). The alamethicin-permeabilized microsomes were recovered on filters and washed as above. More than 95% of microsomal protein was retained by filters in cases of both native and alamethicin-permeabilized vesicles, indicating that the alamethicin treatment did not affect the vesicular structure of microsomes. The alamethicin-permeabilized microsomes retained amounts of radioactivity <20% of that associated with untreated microsomes. Intravesicular radioactive compounds were bona fide lost during the washing procedure because the alamethicin nonreleasable portion did not further decrease even after extensive washing of filters (and microsomes). This allowed us to regard the alamethicin-releasable portion of radioactivity as intravesicular.
Enzyme activities.
All enzymatic assays were performed in buffer A containing 1 mg/ml microsomal protein at 37°C. Permeabilized microsomal vesicles were treated with the pore-forming alamethicin (0.05 mg/mg protein) immediately before the assay. UDP-glucuronosyltransferase activity was measured by using p-nitrophenol as substrate (0.5 mM) in the presence of 3 mM UDP-glucuronic acid. Absorbance of p-nitrophenol was measured spectrophotometrically at 400 nm, at time 0 and 10 min of incubations. Activity was calculated on the basis of p-nitrophenol disappearance. Glucose-6-phosphatase activity was measured in the presence of 1 mM glucose-6-phosphate or mannose-6-phosphate for 30 min. The phosphate released by the enzymes was measured with the malachite green reagent (31). Absorbance at 660 nm was measured and compared with sodium phosphate standards. β-Glucuronidase activity was assayed by using either p-nitrophenyl glucuronide or phenolphthalein glucuronide (1–1 mM) as substrate (10). Incubation time was 30 min; the corresponding deliberated aglycones were measured after alkalinization of samples at 400 and 540 nm, respectively.
Immunoblot analysis.
Microsomal proteins were separated by 11% SDS-PAGE and transferred to PVDF filter membranes by electroblotting. Lowfat milk (5%) in PBS containing 0.5% (vol/vol) Tween 20 solution was used for blocking and for dissolving the primary antibody, which was applied for 1 h at room temperature. Anti-Sec61 (catalog no. PA3-014, Affinity Bioreagents) rabbit polyclonal antibody was detected using a fluorescein-conjugated anti-rabbit Ig-specific secondary antibody. To amplify the signal an anti-fluorescein alkaline phosphatase-conjugated tertiary antibody was also used (all antibodies were purchased from Amersham Biosciences). Blots were overlaid with the fluorescent ECF substrate (Amersham Biosciences). Fluorescence was detected using a fluorescence imaging system (Typhoon, Molecular Dynamics). The primary antibody labeled a band of the expected molecular weight (38 kDa).
Statistical analyses.
Statistical comparisons were made by ANOVA, followed by a Dunnett's test (Table 1) or by a Tukey's test (Table 3). In the case of Tables 2 and 4 and Fig. 1, paired two-tailed Student's t-test was used.
Glucose-6-phosphate (monosodium salt), mannose-6-phosphate (disodium salt), glutathione, UDP-glucuronic acid, p-nitrophenyl glucuronide, phenolphthalein glucuronide, alamethicin, puromycin, anisomycin, and 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid were obtained from Sigma. d-[14C]Glucose-6-phosphate (300 mCi/mmol) and [14C]sucrose (500 mCi/mmol) were from American Radiolabeled Chemicals (St. Louis, MO). [14C]UDP-glucuronic acid (2.2 mCi/mmol) was purchased from UVVVR (Prague, Czech Republic). [3H]Glutathione was obtained from NEN Life Science Products (Boston, MA). Dithiothreitol was removed from the [3H]glutathione solution by extraction as described by Butler et al. (9). Cellulose acetate/nitrate filter membranes (pore size 0.22 μm) were from Millipore. All other chemicals were of analytical grade.
Puromycin increases permeability of rat liver microsomes to citrate and sucrose.
In the first set of experiments, the effect of puromycin was validated by using membrane nonpermeant compounds. Sucrose and citrate are not metabolized inside the ER; therefore, presumably they do not have specific membrane transporters. The permeation of these compounds was checked by the light scattering method. Addition of sucrose or citrate to microsomal suspensions resulted in a rapid increase in the light scattering signal reflecting a shrinkage of vesicles, which was followed by a very slow decrease in the signal reflecting a the swelling phase due to the influx (Fig. 1). The slope of the second phase was steeper in microsomes preincubated with puromycin (0.5 mM), indicating that puromycin increased the permeability of the vesicles towards both sucrose and citrate (Fig. 1). The calculated slope values upon addition of sucrose were −2.26 ± 0.17 (control) and −4.23 ± 0.30 (puromycin) upon addition of citrate were −0.41 ± 0.28 (control) and −0.78 ± 0.19 (puromycin). Values are expressed as arbitrary units per minute, means ± SD. In both cases, the slope in puromycin-treated samples differed significantly from that of controls (P < 0.01). Puromycin concentrations >0.5 mM did not result in a more pronounced effect (data not shown).
To control whether this increase in permeability can really be attributed to the translocon pore (and not to a nonspecific effect on the membrane), the effect of puromycin was evaluated either in total (nonfractionated) and smooth microsomes, in which translocons are underrepresented. The uptake was measured with rapid filtration technique, by using radiolabeled sucrose. The basal rate of sucrose uptake was higher in total compared with smooth microsomes, calculated either on protein (Table 1) or on phospholipid basis (data not shown). Puromycin (0.5 mM) caused an increase in the rate of sucrose uptake of total microsomes only (Table 1).
Puromycin increases uptake of UDP-glucuronic acid in rat liver microsomes.
The uptake of various anions by rat liver microsomes was detected by using radiolabeled compounds by rapid filtration. The control time course of UDP-glucuronate (0.02 mM) influx showed the characteristic initial overshoot, followed by a decline, as it has been reported by others (7). A completely different curve was gained in the presence of puromycin (0.5 mM); both the overshoot and the declination phases disappeared and an equilibrating uptake was detected (Fig. 2). Puromycin was ineffective in case of glucose-6-phosphate and glutathione transport (Table 1). In case of glutathione a higher (0.4 mM) concentration should had to be used; at comparable (0.02 mM) concentration uptake could not be properly detected due to a high background caused by binding.
Puromycin promotes substrate access to intraluminal enzymes of ER.
UDP-glucuronosyltransferases (13), glucose-6-phosphatase (41), and β-glucuronidase (42) are ER enzymes with active sites located in the lumen. Hence, the ER membrane represents a barrier for their substrates; consequently, the activity of these enzymes is reduced in native microsomal vesicles. Agents that disrupt the integrity or increase the permeability of the membrane cause an increase in the activity of these enzymes.
Microsomal UDP-glucuronosyltransferase activity was measured by using p-nitrophenol and UDP-glucuronic acid as substrates. The activity in native microsomal vesicles was less than 10% of the total activity measured in permeabilized vesicles (Table 2). The addition of puromycin resulted in a concentration-dependent increase in the activity (Fig. 3); 30% activation was observed at 0.5 mM puromycin concentration. Puromycin concentrations >0.5 mM did not cause a more pronounced effect (data not shown). However, puromycin was ineffective in alamethicin-permeabilized microsomes (Table 2), which indicates that the effect was likely due to the improved access of UDP-glucuronic acid the enzyme to the enzyme and not a direct activation of the enzyme itself.
Microsomal β-glucuronidase activity was measured with p-nitrophenyl glucuronide and phenolphthalein glucuronide as substrates. In accordance with previous results (10), the enzyme had a moderate latency in control vesicles. Puromycin did not increase β-glucuronidase activity (Table 2).
The catalytic subunit of glucose-6-phosphatase is able to hydrolyze hexose phosphates other than glucose-6-phosphate (41). The specificity of glucose-6-phosphatase activity is guaranteed by an ER transport protein, which is strictly specific to glucose-6-phosphate (14, 15, 19). Consequently, the latency of the enzyme is lower with glucose-6-phosphate as substrate than with other hexose phosphates, e.g., with mannose-6-phosphate (8). Therefore, changes in hexose-6-phosphatase activities upon puromycin addition were monitored by using both substrates.
The addition of puromycin resulted in a concentration-dependent increase in mannose-6-phosphatase activity (Fig. 3). A maximal activation of ∼15% was observed. Puromycin was ineffective in alamethicin-permeabilized microsomes (Table 2), which argues against a direct activation of the enzyme. Puromycin was unable to increase the activity when glucose-6-phosphate was used as substrate (Table 2 and Fig. 3).
Effect of puromycin on UDP-glucuronosyltransferase activity can be prevented by a translocon-binding antibody, by puromycin antagonist anisomycin, and by release of ribosomes.
It has been reported that an antibody (or its proteolytic fragment) binding to the Sec61 protein of the translocon occludes the pore of the channel (17). Therefore, the translocon-specific effect of puromycin was controlled by investigating the effect of this antibody on UDP-glucuronosyltransferase activity. Specific binding of the antibody was checked by immunoblot analysis of the microsomal proteins (data not shown). As expected, the addition of the antibody to microsomes counteracted the effect of puromycin. Moreover, it decreased the activity of UDP-glucuronosyltransferase even in the absence of puromycin (Table 3). The inhibition was specific, since neither the heat-denatured antibody, nor an anti-rabbit secondary antibody, was effective (Table 3).
Anisomycin is a known antagonist of puromycin (21), which is effective only if added beforehand. Anisomycin has been successfully used in microsomal experiments to prevent various translocon-dependent effects of puromycin (27, 33). In our experiments, puromycin failed to increase UDP-glucuronosyltransferase activity when added after anisomycin. If anisomycin was added after puromycin, it was ineffective (Table 3).
The release of ribosomes from the surface of microsomal vesicles also prevented the effect of puromycin. Pretreatment with EDTA (5 mM), which is known to detach ribosomes (12), decreased UDP-glucuronosyltransferase activity by 37%, and completely abolished the effect of puromycin. As shown in Table 3, puromycin was also ineffective in microsomes pretreated with a high-salt buffer, another maneuver that also removes ribosomes from the microsomal membrane (30). Treatment of native microsomes with high-salt buffer did not affect UDP-glucuronosyltransferase activity; in permeabilized microsomes the buffer slightly increased the activity (data not shown).
Different membrane permeability in smooth and rough microsomes.
Because recent studies (30, 34), have indicated that nontranslating empty ribosomes are present on the surface of the ER, this condition may therefore contribute to the permeability of the ER membrane. By using the above approach, we compared the latency of UDP-glucuronosyltransferase, glucose-, and mannose-6-phosphatase activities in rough (i.e., translocon rich) and smooth microsomal fractions. All of these enzyme activities were present in both microsomal fractions (Table 4). The latency of UDP-glucuronosyltransferase activity was much greater in smooth vesicles. The latency of glucose-6-phosphatase enzyme measured with glucose-6-phosphate was similar in the two fractions. However, when mannose-6-phosphate was added as substrate, a higher latency was found in smooth microsomes (Table 4).
The present findings show that translocon protein channels can mediate the permeation of small molecular weight anions (besides calcium ions and neutral polar molecules) across the ER membrane. As reported previously (35, 36), the translocon may permit the transmembrane flux of small anions, such as glutamate and HEPES. In the present study, the membrane permeation of six anions of different size and shape (glucose-6-phosphate, mannose-6-phosphate, glutathione, UDP-glucuronic acid, phenolphthalein glucuronide, and p-nitrophenyl glucuronide) was examined. All of these compounds are (or can be) metabolized by enzymes with an intraluminal activity, therefore, a possible contribution of the translocon channel to their membrane transport has a physiological relevance.
Puromycin was used to empty the translocon channel by releasing the incomplete nascent polypeptide from the ribosome. The effect of puromycin was specific because 1) it was absent in the smooth microsomal subfraction poor in translocons (Table 1.); 2) it did not cause a generalized permeability of the vesicles (Fig. 3 and Table 2); 3) it was ineffective in permeabilized vesicles (Table 2); and 4) it could be antagonized by anti-Sec61 antibody, by anisomycin, and by the release of ribosomes from the membrane (Table 3).
The puromycin concentrations used in our study were similar to those in previous studies, and the effective concentrations were in good agreement with other observations. In a recent study, Van Coppenolle et al. (40) applied 200 μM puromycin. Others have used similar concentrations (100–1,000 μM) to estimate the puromycin effect on ER permeability (18, 27, 33).
Although the pore of the translocon complex in the ER is large enough to be permeated by small molecules, it is generally believed that permeation is prevented by a barrier constituted by BiP at the luminal end of the pore (17). A recent study (18) rejected this assumption and concluded that a small, neutral molecule can permeate the empty pore of a translationally inactive, ribosome-bound translocon even in the presence of luminal proteins. Our findings support this observation and affirm that the translocon could provide a pathway for small neutral and anionic molecules to cross (although slowly) the ER membrane.
The effect of puromycin on anion transport is controversial at first sight. The permeation of mannose-6-phosphate and UDP-glucuronic acid was increased upon puromycin addition (Figs. 2 and 3 and Table 2), whereas in case of glucose-6-phosphate, glutathione, p-nitrophenyl glucuronide and phenolphthalein glucuronide, no effect could be demonstrated (Fig. 3, Tables 1 and 2). The discrepancy cannot be explained by the different size or charge of the indicated compounds; e.g., mannose-6-phosphate and glucose-6-phosphate are epimers. A more reasonable explanation is that the contribution of the translocon to the transmembrane flux is remarkable in cases when other possibilities for membrane transport are absent (or have a very limited capacity). Indeed, a high capacity ER transporter has been already characterized for glucose-6-phosphate (14, 15, 19). Glutathione (2) and glucuronide (1, 4, 11) transporters are also known, although they have been explored only functionally. On the other hand, no transporter for mannose-6-phosphate has been even hypothesized in the ER, and the existence and functioning of the ER UDP-glucuronic acid transporter is controversial (1, 7).
This explanation is supported by the finding that puromycin exerted only a slight increase even in cases when it was effective. Effects of similar magnitude have been found in previous studies on calcium (27, 40) and 4-methylumbelliferyl-glucoside permeation (18). However, it does not necessarily mean that translocon channel plays a minor role in the permeation across the ER membrane, because the basal rate of transport (i.e., in the absence of puromycin) observed in microsomal vesicles might well be due to translocons unoccupied by polypeptide chains. The difference in permeability between smooth and rough microsomal vesicles (Table 4) seems to favor this hypothesis.
The flux of low molecular weight compounds through translocon channels can be regarded as a structural imperfection which alters the barrier function of the ER membrane. Nevertheless, it might have a physiological function as a low-affinity, low-capacity transporter with low selectivity. A variety of structurally unrelated compounds are (or should be) able to cross the ER membrane, but only a few transporters have been reported so far (26). For example, various xenobiotics should reach the luminal compartment of the ER to be glucuronidated. Translocon channels may have a role in their transport. Moreover, nucleotides and nucleotide sugars are usually transported by antiport mechanisms in the ER (6, 22). The counter ion in these cases can be supplied by translocon. The role of the translocon has been also hypothesized in the transport of such a bulky anion like FAD (39) or in the permeation of exogenous biotin derivatives (26).
In summary, the translocon channel mediates the permeation of low molecular weight anions in the ER. The phenomenon may play a role in the substrate supply and/or product elimination of some intraluminal enzymatic activities in the ER.
This work was supported by the Italian Ministry of University and Research Grant RBAU014PJA, the Hungarian Ministry of Health Grants ETT 090/2003 and 613/2003, National Scientific Research Fund Grants OTKA F37484, F46740, T48939, T38312, and TS49851, and a grant from University of Siena (quota progetti; to A. Benedetti).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2006 the American Physiological Society


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Get in the Christmas Holiday spirit with these fun Disney Coloring Pages. Click the image to open in a new window and save to your computer. Check out these fun Disney coloring books on Amazon. By clicking these links you will be using my affiliate code and I will receive a small kick back on your purchase which helps with running my blog.
Disney Christmas Coloring Pages
Mickey's Very Merry Christmas Party
Advertisement Can you feel the chill in the air? The excitement? Christmas will be here before you know it, and I can't think of a better place to spend it than in the Walt Disney World Resort in Orlando, FL. Walt Disney World has always been a magical place, but they really outdo themselves every year during the holidays. Strands of garland hang above Main Street USA, a festive tree adorns the entrance to every park, carols can be heard {Read More}
Disneyland During and After Christmas
The Happiest Place on Earth Just Got is Still Merrier. Did you miss out on going to the Disneyland Resort before or during Christmas? No need to worry, there is still time to visit the park and experience all the holiday decor and fun. Cars Land, Buena Vista Street and Main Street will be decorated until January 6th. Sleeping Beauty's Winter Castle is decorated with 80,000 LED lights to create that special magical sparkle at night. The 60 foot Christmas {Read More}
Disney ¡Viva Navidad!
The Happiest Place on Earth just gets merrier this holiday season with the newest celebration at the Disneyland Resort – "Disney ¡Viva Navidad!" Guests will enjoy this festive holiday presentation now through Jan. 6, 2014 in Disney California Adventure Park. "Disney ¡Viva Navidad!" is a merry celebration inspired by the warmth and joyous spirit of the Latino culture, filled with holiday traditions, jubilant dancing and exciting music. Based in and around the Paradise Garden area of Disney California Adventure, the {Read More}
Christmas at the Carthay Circle Restaurant
Welcome to those of you joining me from Disney on Wheels and those of you just hopping aboard. I am the 3rd stop on our Magical Blogorail. This month we are blogging about the restaurant in the Disney Parks that feels most like a Christmas dinner. I am on the West Coast and Disneyland Resort is where I frequent the most. If I was at the Disneyland Resort for Christmas I would dine at the Carthay Circle Restaurant inside {Read More}

subset: commoncrawl


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Owned since new?
Owned for how long?
0.5 year(s)
Buy this make again?
Price and equipment10.0/10
Petrol is not a concern with this car, even when fuel prices are soaring.
Servicing and running costs2.0/10
I'm not sure about this one because I haven't owned the car long enough yet. But they are a popular car so I can't imagine services and running costs would be too extravagant.
Aesthetics and styling10.0/10
It's a very cute little car, great to get around busy car parks and city streets.
Overall verdict8.0/10
This Mazda 121 is everything I wanted in my first car. Fuel and running costs are low and Mazda is a reliable brand. It is great to drive in residential streets, city streets and even on highways. Being such a small car, I was nervous at first when taking it on the highway, but to my surprise it was great! The boot space is very deceiving and actually can fit quite a lot of luggage.


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Google Apps EDU Certification Sessions
At this summit we will be offering a Google Apps EDU Certification track.
Allison Mollica
Allison Mollica has a background in both business and education. As a Google Apps for Education Trainer, Google Apps Certified Administrator , Allison is an international Google Summit featured speaker and global trainer. She is also a Virtual Instructor for NH's Virtual Learning Academy, with M.Ed Technology in Education from Lesley University. She works with school by developing customized approached to deploying and leveraging Google Apps for Education in 'their environment.'


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ogti9g9's Feedback
All right, to start this off, i must say i deserve a minus one...
Why you ask? Well, i recently posted a Murder inc bko up for sale...
I realy liked this marker, but i never was able to get it to work, so i decided
to put it up for sale. Another member and me began to converse about the sale of this marker, he wanted it, and everything was pretty much set in stone. Before getting it packed and ready to be shipped, i went through it and checked all of the internals and parts to make sure they were all fine. I found out that a line was jammed with lube, so i removed it and placed a new internal line there. Put it back together and aired it up, and it works!!! I have had this marker for roughly a year and a half and have had to replace pretty much every internal piece in order to get this marker to work. Ive had to replace the Hpr, lpr, ram, hoses, valve, orings, ive had to ship it up to virgiania to get it checked out, and have spent around $180.00 to get it to work... Upon getting it to work, i contacted the member and told him to cancel paypal payment because of my over joyous moment in getting this marker to work. I am giving myself bad feedback because of this, i apoligize to the other member, and respect his anger towards me, but this is a relief to have this marker working. That is all.
Traded an LCD charger for an LED charger. Smooth as can be and i would not hesitate to deal with again!
we traded regulators and he shipped first and was a great person to deal with A+++
Bought an fs4, came exactly as described.
traded my timmy for 2 angels
Easy to work with, great communication, thanks!!
Traded a psp + games for his angel. Shipped when he said he would & the angel came as described. Would deal with him again without hesitation.
bought a shoebox shocker off of him. Got a great deal. the shipping was fast and it was also well packed. Will do business with again. Thanks buddy
ogti9g9 Feedback
Great experience with this seller. Got a good marker at a good price shipped next day. Nothing but good things with this deal.
Bought a red autocker barrel from me paid fast and waited for it to arrive after it was shipped for 7-10 day shipping. A+++
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Zac General Guide by wolfsfang
Not Updated For Current Season
This guide has not yet been updated for the current season. Please keep this in mind while reading. You can see the most recently updated guides on the browse guides page.
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AP Tanks
Zac Build
start with this
follow with these
to make sure they pay for touching the goods
damage for the killings to be had
Ability Sequence
Not Updated For Current Season
The masteries shown here are not yet updated for the current season, the guide author needs to set up the new masteries. As such, they will be different than the masteries you see in-game.
Welcome To The Crazed Mind That Is Me...
Welcome this is my first guide, I did well with this build if you do well or just like it +1 it if you don't oh well...
Pros / Cons
+he has the greatest mobility
+built in escape mechanic
+an AP with no mana
+excellent sustain
+you have 3 CC's
+you can tower dive like crazy and live
+big cooldown
+moves consume health
+your blobs can be killed
+blobs from cell division can be smited
Cell Division if used correctly can save you from a losing battle fast
-this is also your greatest offence if you plan it correctly you can make what would have been a death a multi-kill.
-if your close to a wall the blobs spawned from it practically appear on top of your corpse and go in in a matter a of seconds.
Stretching Strike is your Q's CC
-it slows so be sure to use it quick to escape champs.
Unstable Matter is an aoe based on your magic damage and their health
Now the meat & potatoes of the skills
Elastic Slingshot your second CC and by far the best.
-this one does knock back, great for interrupt's
-this ability can be used for both quick escapes or diving for a kill
-i know it can be interrupted but don't be to scared let it charge a bit to get out of a predicament
And last but not least Lets Bounce your last CC
-this one does 4 things first you gain MOVEMENT SPEED second it DAMAGES third it SLOWS and last it does KNOCKUP.


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William Shatner Confirms Potential Role in Developing 'Star Trek 3'
by Ethan Anderton
September 29, 2014
Source: Trek Movie
Just last week, a rumor was going around the Star Trek 3 might try to get William Shatner back into the franchise he helped make a pop culture icon with some kind of cameo role that would team him up with Leonard Nimoy again reprising his role as Mr. Spock. Director Roberto Orci wouldn't confirm or deny the possibility, but now, thinking that the cat was officially let out of the bag, Shatner himself has gone and confirmed the potential return of Captain James T. Kirk. Speaking at Wizard World Nashville (via Trek Movie), Shatner was asked about his involvement in the new sequel, and explained how the role came up.
Here's a transcript of what Shatner said, referencing a previous attempt at a cameo in 2009's Star Trek:
"The weirdest thing has happened and I think I can tell you because it is so weird. So I'm in Australia a couple of weeks ago…and J.J. Abrams calls me. He called me in Los Angeles and I'm not there, so my assistant calls me and says 'J.J. called and is going to call back.' I said 'great' and hung up the phone 'I wonder what J.J. wants.' Because the last time I talked to J.J. was before the first movie came out and I went to meet him at his office and he said "are you interested in being in the Star Trek movie?" 'And I would play the captain?' 'No, no. Not as Captain Kirk. We have a young, handsome guy to do that.' But he handed a script and I read it, and it stunk…It is like telling people 'You know that little cute baby you got there. It is the ugliest thing I have ever seen!' So I didn't say that to J.J.. This is the worst script I have ever read and it is the next 'Star Trek' movie and the beginning of the whole thing and it was awful! Turns out it wasn't the script at all…He was assessing whether people were interested in being in the movie and handing them a dummy script because the final movie was really good, but it wasn't the script that I read.
So now I get this call and he is going to call me back and a week and a half goes by and he never called me. I wonder if he is going to call me? Maybe it is a trick? Maybe it is TMZ? Could he be that cruel. So he calls and says 'I'm calling, Bill, because the director of the Star Trek movie, the next one, has had an idea where you might be involved in it. So I am calling to see if you would be interested.' I said 'Oh, yeah. If it is meaningful.' When Leonard was Spock in that first movie, I said to Leonard "you know when you go back in time you are still old." So it depends on what you do with the character. I said "I would be delighted." And he said "you can't say anything." And I said "I wont even tell my wife. I wont tell anyone. I wouldn't do that, J.J.."
So I get back from Australia and it is all over the Internet, that the director held an interview and said they wanted Shatner and Nimoy to be in the next movie. I bet J.J. is frothing at the mouth. So the news is out that they have an idea that they want Leonard and myself. They might want Leonard and myself . I don't know what to do with it. How do you get me fifty years later into the movie…I know it's science fiction, but even I couldn't come up with an idea."
So there you go. That's official confirmation from the original Captain Kirk himself that there's a chance for him to reprise his iconic role. It sounds like Shatner will have to see the script first before making a final decision, and we're interested in seeing how the writers bring the old Captain Kirk into the mix. The original Mr. Spock is around in the new timeline because of a wormhole, so it's not hard to imagine that Kirk could end up in this timeline in the same fashion, but how would he end up getting caught up with his old friend and their younger selves? Here's hoping they have a good idea to make this a reality. Thoughts?
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With Its Official Song Released in 2016 and Its Visuals Directed by MEX, Ric Hassani Has gotten Multiple Thumbs up Nigerian Celebrities, Ice Prince & VJ Adams Decided to Spit Bars and Jefak Decided to Make the three Songs One with Some extra 'Efizzy'. Gentleman is the rave for the Movement, Stop Dancing for a Moment, You need to Ponder with this Lovely Track & Listen to the End.
Download & Enjoy.
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Bentley for Sale
Selected Filters:Type of Ad: Vehicles for Sale Make: Bentley
1956 Bentley Gm Conversion Limo
Stratford, NJ
Just arrived 1956 Bentley Silver I Cloud used as a Limo Service for Wedding and Special occasions. Now this is the way to go... More
1962 Bentley S3 Limousine
Stratford, NJ
This Bentley has been religiously maintained by a reputable Palm Beach Art Collector. Service Records ( available with Rolls Royce or Bentley Grill )... More
1958 Bentley S1 S1
Stratford, NJ
Just up on Consignment 1958 Bentley S1 4 door sedan with pull out Pick-nick Tables.....Original tool kit included!! Just 62,186 miles, imported into US... More
2004 Bentley Arnage T Sedan
Delray Beach, FL
This Clean 2004 Bentley Arnage T has a shining SILVER exterior and a very clean GRAY leather interior with 67K miles! The ultimate Autobahn... More
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2002 Bentley Continental R Mulliner "Wide Body"
Montreal, QC
NOTE: All of our prices are listed in US Dollars for clarity. Many pictures are available on our website, but if you need more, or... More
1998 Bentley Continental T
Saint-Leonard, QC
NOTE: All of our prices are listed in US Dollars for clarity. Many pictures are available on our website, but if you need more, or... More
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For over 40 years, John Scotti Automotive has been the reference in the field of exotic, vintage and collector cars in Montreal, Quebec and... More
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2000 Bentley Arnage Red Label This very presentable 2000 Bentley Arnage Red Label shown here in is available in a beautiful navy blue with tan... More
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Marino Performance Motors is thrilled to announce the arrival of this 1 OWNER 2013 Bentley Continental GT Convertible V8 finished in stunning and very... More
Advertiser since 2013


text:
The Regulator Bookshop Presents
John Manuel reads from his celebrated novel of Durham—Hope Valley. Plus Dance to The Bull City Beatniks & The Chit Nasty Band
Fri Apr 7
Doors: 6:30 pm / Show: 7:00 pmMotorco Music Hall
This event is all ageshttps://www.motorcomusic.com/event/1456790/
Chit Nasty
Ecstasy. It fills the faces of fans at the live shows, emanates from the bodies shaking to the powerful rhythms of three guitars, drums, and keyboards. Plain and simple: this band makes people happy. The Chit Nasty Band is the creation of Christian "Chit" Foushee-Green, singer, songwriter, and keyboardist extraordinaire. Raised in the music of the black church, Foushee-Green knows how to inspire an audience through upbeat lyrics, onstage dance moves, and expert musicianship. His trademark sound harkens back to the funk bands of the 70s and 80s, but with a contemporary flair that reflects the vibrant music scene of Chapel Hill/Durham, North Carolina. Foushee-Green has assembled around him a group of expert musicians whose steady, powerful sound gives him the platform on which to freelance. Bassist Michael Bare, a veteran of Lucky Strike, Jessica Long and the New Kind, and The Chris Hendricks Band, creates the foundation with bass lines guaranteed to loosen up the tightest bod. Guitarist Jackson Manuel adds soaring, precise leads, and backing vocals that rival any of today's professional singers. Trading leads and vocals with Manuel is guitarist John Faltass. John's Sphinx-like expression belies a playful soul whose aim is "to put your head in the clouds while keeping one of your feet on the ground." Drummer Evrick Lee, "E" for short, joined the group in 2013. Creative and upbeat, E is described by the band as "the drummer we were always looking for." Together they create a sound that will make you feel good deep down in your soul and lift you up higher and higher. Welcome to the Nasty Nation!
Bull City Beatniks
We are a rock trio consisting of John Manuel, bass and vocals, S.D. Williams, guitar and vocals, and Aisander Duda, drummer. Joining us on stage will be guitarist Gregg Kreizman, guitarist and vocalist Jackson Manuel, vocalist Stacey Anderegg, and keyboardist Chit Nasty.


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Saki Santorelli, "Heal Thy Self: Lessons on Mindfulness in Medicine"hat is darkest and brightest within each one of us.
2010 | ASIN: B003DYGO8C | ISBN: 0609805045, 060960385X | 290 pages | EPUB | 3 MB
2010 | ASIN: B003DYGO8C | ISBN: 0609805045, 060960385X | 290 pages | EPUB | 3 MB
"Perhaps our real work, whether offering or seeking care, is to recognize that the healing relationship--the field upon which patient and practitioner meet--is, to use the words of the mythologist Joseph Campbell, a 'self-mirroring mystery'--the embodiment of a singular human activity that raises essential questions about self, other, and what it means to heal thy self."
--Saki Santorelli
Today we are experiencing extraordinary technological advances in the diagnosis and treatment of illness while at the same time learning to take more responsibility for our own health and well-being. In this book, Saki Santorelli, director of the nationally acclaimed Stress Reduction Clinic, explores the ancient roots of medicine, and shows us how to introduce mindfulness into the crucible of the healing relationship, so that both patients and caregivers begin to acknowledge that we are all wounded and we are all whole. His approach revolutionizes the dynamics of the patient/practitioner relationship. In describing the classes at the clinic and the transformation that takes place in this alchemical process, he offers insights and effective methods for cultivating mindfulness in our everyday lives. As he reveals the inner landscape of his own life as a health care professional and we join him and those with whom he works on this journey of human suffering and courage, we become aware of and honor what is darkest and brightest within each one of us.
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Magic recharge coupons
Bharti Mobitel has introduced a Rs 350 recharge coupon for pre-paid Magic subscribers. One can avail of talktime worth Rs 263 that will be valid for 15 days. It has also announced another value-added proposition for Magic users by waiving the processing fee of Rs 220 and Rs 300 on the Rs 1,200 and Rs 2,000 recharge coupons, respectively. The offer will be valid for a limited period. The validity period of 60 days and 90 days, respectively remains the same. We have also extended the validity period on the Rs 650 recharge coupons from 30 days to 45 days, says chief operating officer Deepak Gulati.
ACC dispatches
ACC Ltd has recorded a 4.49 per cent rise in its cement despatches at 10.69 lakh tonnes for November 2002 compared with 10.23 lakh tonnes in the same month last fiscal. The cement production, however, was flat at 10.65 lakh tonnes last month, a company release said.
Ivega move
Bangalore-based software company, Ivega Corporation has announced its merger with Amoolya Technologies. As per the terms of agreement, Ivega will assume the support contracts of Amoolyas customers and partners. All trained and skilled manpower will be absorbed into the company.
Canara arm
Canbank Factors Ltd, a subsidiary of Canara Bank, has reported a 8.61 per cent rise in net profit at Rs 4.54 crore for the half year ended September 30, 2002, compared with the corresponding period last year. It has achieved a factoring volume of Rs 568.16 crore for the period, a 30 per cent growth.
HPCL launch
Hindustan Petroleum Corporation Limited (HPCL) has launched its co-branded two-wheeler petrol engine oil in association with Kinetic Engineering Ltd. The oil, Kinetic Genuine Racer is available in two variants for 2-stroke and 4-stroke engines.
Reliance plan
Reliance Capital Mutual Fund will launch Reliance Short Term Fund on December 9 targeted at investors with short-term surpluses. The scheme would invest in short-term debt and money market instruments and does not have any provision to invest in equity related instruments. It would have no entry and exit load and will close for subscription on December 17.
Huawei Tech
Huawei Technologies has announced the signing of strategic agreements with NEC Corporation and Microsoft Corporation to set up joint labs in China.
Wipro Infotech
Wipro Infotech has received the gold certification from Cisco Limited, for relentless pursuit of customer satisfaction, training, support and specialisation requirements set by Cisco.
Mount Shivalik
The Mount Shivalik group will invest about Rs 40 crore for setting up a brewery in Bihar for producing its Thunderbolt brand of beer.


text:
In a new monthly series, menu trend analyst Nancy Kruse and NRN senior food editor Bret Thorn debate current trends in the restaurant industry. This installment takes a look at how restaurants are featuring fruit.
New techniques make for better tasting fruit offerings
NRN senior food editor Bret Thorn presents his take on fruit trends at restaurants, from local-seasonal offerings to cold-pressed juices.
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Communities rally to fight back against heroin
John Charity Spring Back Home in Davis County, UTApril 20, 2014 7:41 p.m.
This article points out the sad fact that a heroin epidemic is destroying America's communities. However, it fails to place blame on one of the major contributors: modern Hollywood.
The irrefutable fact is that modern Hollywood has glamorized heroin and made it the hip drug for America's youth. Indeed, Hollywood has made heroin appear to be the only way to achieve fame, fortune, and sexual conquest.
Hollywood's portrayal of heroin as harmless recreation has ruined literally millions of lives. Shame on Hollywood nod it's liberal agenda.


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The wretched January transfer window has thankfully come and gone but have Blackpool finished the month in a stronger position than they started it?
The farcical and almost slapstick nature of deadline day meant that we had to wait until half an hour after the 11pm cut-off point for Blackpool to complete their business.
There wasn't a great deal the club could have done about that though, with Port Vale waiting until the very last minute to conclude a deal for Blackpool's club captain Danny Pugh.
It was a surprising bit of business that caught many off guard. I have to admit the Neil Danns deal caught me by surprise, but losing your skipper – who only signed during the summer – was fairly baffling too.
For me, it seemed to sum up Pool's transfer policy. For those asking: "what policy?", there lies my point.
Pool's window seemed a bit haphazard, last minute and if I'm being brutally honest, a bit all over the place.
At one point in January, the Seasiders had six senior players out through injury, which seemed to force Gary Bowyer into action.
Deals for Nathan Delfouneso and Sanmi Odelusi were sharply wrapped up but it wasn't until the last two days when the majority of business was done.
Raul Correia looks like an interesting one for the future but it leaves Pool with eight forwards – counting Danny Philliskirk and Odelusi. Isn't that a bit top heavy?
Not only that, Pool were already crying out for defensive reinforcements before Andy Taylor and Eddie Nolan received injuries and Kelvin Mellor was handed a suspension for his red card at Blackburn.
Taylor and Nolan are now back in contention, but if the Seasiders are to persist with the 3-5-2 formation they've adopted in the last few weeks, extra defenders were a must. Yet, curiously, no defenders were brought in.
The main worry was that Kyle Vassell, Brad Potts and Bright Osayi-Samuel could be sold but they're still here, which is a major boost.
One accusation aimed at the club before the window opened was that they – and when I mean "they" I refer to the owners – may look to cash in on their brightest talents to recoup some money.
That didn't happen, but many are still accusing the club of letting senior players go to cut down the wage bill.
The real truth of that may never be known but I'd be surprised if Bowyer was entirely satisfied with Pool's January shopping.


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How to Be a Wicked Woman(2004)
A collection of stories by Susanna Carr, MaryJanice Davidson and Jamie Ann Denton
The Wicked Witch Of The West Side - Mary Janice Davidson New York publisher Karen Desjardin has been driven to fits of temperamental insanity by clueless bean-counters and bad writing. The only person who isn't afraid of Karen is her publishing house's best selling romance star-Hope Desiree LaFrance, a.k.a. hunky writer Steven McCord. And he has a few plans to redeem his wicked editor...he'll start by planting kisses on those full lips and take expert editorial direction from there...Instruction In Seduction - Jamie Denton Eden Matthews is a good cop with a bad reputation...for being a zero between the sheets. Cop-on-the-edge Jackson Hunt has a bad reputation...for being an absolutely legendary lover. Determined to tap into her inner vixen, Eden blackmails Jackson into teaching her what it takes to be a wicked woman...Wicked Ways - Susanna Carr Faking a wild life for the sake of her clients currently has Peyton Lovejoy in the bathroom stall of a male strip joint, making a frantic call to...the 24-hour public library hotline. Mike Ryder is certainly intrigued by Peyton's desperate questions (How does one tip a guy in a thong?) and completely captivated by the idea of answering all her sexually charged questions...
Used availability for Jamie Ann Denton's How to Be a Wicked Woman
Paperback Editions
August 2004 : USA Paperback


text: I have written this guide looking at SEO in 2016 with the goal of being accessible to everybody. You may never have built a website before, you may have had one built for you. You may even be an SEO wizard. My goal was to make this guide different - full of useful, actionable, powerful advice which is objective. Most importantly a lot of this information is unique - you won't find these tactics … [Read more...]


text:
Published: Monday 26th January 2015 by The News Editor
Residents from Hull and East Yorkshire should be bracing themselves for snow this week, new Met Office forecasts suggest.
They have been warned to expect possible travel disruption as the cold snap sees the snow sweep in alongside blustery winds.
People in northern England are being urged to be especially careful when they venture out by the Met Office, whose yellow warning covers the region, including Yorkshire.
Wednesday and Thursday are likely to see the worst of the weather, with Scotland, Northern Ireland, the Midlands as well as areas of eastern England also hit.
Snowfalls of between three and 6cm are expected in higher areas of the north of England.
Public Health England's Angie Bone said that adults of working age are most at risk this week. Dr Bone recommended people wear slip-resistant footwear, which could help stop any falls on the ice.
Copyright Press Association 2015
Published: Monday 26th January 2015 by The News Editor


text:
1993 Volvo 960 - Consumer Review
Build Quality
Reliability Value
Build Quality
Reliability Value
One ole tank
By Dr. Psy.D on
Vehicle1993 Volvo 960 4dr Sedan
ReviewI bought the car with 84,150 miles when it was 16 years old. No problems other than minor: check engine light, sunroof is stuck, and other minor details mostly due to age. With premium gas and adhering to speed limits driving steadily even with a/c on I get nearly 26mpg. Power is good despite the weight and age and car insurance rates are pure joy. The car feels safe and predictable at all times, comfort and noise level are superb, and so are the turning radius, oversight, and trunk space. Due to extreme UV the leather seats are a bit dried out but I am definitely sold on Volvo and hope it will last much longer.
Best FeaturesVery comfortable seats, great overall sight and turning radius, quiet, safe, inexpensive to insure, and very solid
Worst FeaturesMore durable leather materials, should have built them much longer :)
Recommend (0) (0)
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text:
How To Make A Beautiful Lace Crown
I've always loved crowns, they're my absolute favorite! Maybe it's because the name Candace "was the title for queens and queen mothers of the ancient African Kingdom of Kush, also known as Nubia and Ethiopia?" Or perhaps I watched far too many Disney movies as a little girl! Whatever the case may be, I adore crowns and I can't wait to make this gorgeous lace crown DIY expert Tanya Memme created on Hallmark's "Home & Family" this week. Isn't it absolutely gorgeous? This crown would be such a fun craft project for girls of all ages (Tanya's adorable three year old daughter, Ava created one as well) and is the perfect decor in every regal inspired home or party!
Lace Crown DIY Tutorial
You Will Need:
- Lace 12-15″ each
- Fabric stiffener or modpodge
- Silver, bronze, gold, pink spray paint or acrylic paints (whatever color you wish)
- Pen
- Glue/glue gun
- Thin metal hair band
- Wax paper
- Choose your lace. You can use synthetic, cotton or whatever you wish. Cut them into 12-15″ pieces, depending on how you want them to sit on the head.
- Put on latex gloves and saturate each piece of lace in fabric stiffener. Then, wipe off excess, and make sure clear all bubbles or "windows" formed in the holes of the lace by the glue. Blot off excess glue.
- Set each piece out on a sheet of wax paper, turning over a few times to help them dry faster. Repeat this process 2-4 times depending on how well the type of lace takes to the stiffening effect.
- Let dry until it isn't tacky to the touch, after the fabric stiffener is dry then spray paint or paint with a brush or sponge or use a paint pen to bring color to both sides of the lace. Let the paint dry.
- Bring the two edges together matching up the pattern and glue with a glue gun into a crown shape. Place on the head with bobby pins to secure!
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Dear Josh,
You are ALL boy and find the biggest joys in this life to be when you are getting into something you aren't supposed to. Jules tends to mind us "pretty well", and she will grow up to be a people pleaser one day. You? You are a sweet, loving, kind hearted little boy who likes getting into trouble. You test the boundaries we set and think it's hysterical. These pictures are from where you snuck into the living room and decided to climb up on the couch and jump up and down - a big no-no. However, you were the happiest little boy in the world, so I had to grab my camera and take a few shots of your sweet giggly face. Sometimes its ok to be bad. After all, "If it makes you happy...it's can't be that bad" - right???


text:
GraphicRiver Infographic Businessman Hand Hold Business Bag 7549096
Category: Infographic | Comment: ( 0 )
GraphicRiver Infographic Businessman Hand Hold Business Bag 7549096
Presentation \ Object \ Modern \ Infographic \ Information \ Sign \ Statistics
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ZIP file contains original AI file, EPS file,Files well organized and layered.
The font used is free for commercial use and you can find them here: Fonts: Text and Titles – PT Sans " http://www.fontsquirrel.com/fonts/PT-Sans "
Concept Design
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Infographic Business World Job Template Design; Businessman Up the Head Ladder Paper Cut; Infographics Vector Apple Design Diagram Line; Businessman Up the Arrow Ladder Paper Cut Style; Infographic Template Coffee Bean Jigsaw banner; Infographic Soccer Ball Elements Design Template; Infographic Technology Elements Design Template.
Keywords: abstract, background, bag, business, chart, concept, creative, data, design, education, elements, graph, graphic, hand, icons, illustration, infographic, information, modern, object, pie, presentation, sign, statistics, symbol, tag, technology, template, vector.


text: We need to repaint our wood fence once it warms up out there and I'm just wondering what brand of outdoor paint lasts long but isn't too expensive? we have A LOT of fence to paint so I know it will be costly. (It was painted 10 years ago by the previous owners). We also want to paint the old aluminum siding and shutters on the front of our house. '


text:
Unexpressed feelings can pile up and poison the relationship. When you repress your anger, it will always come out later, usually after something minor has upset you.
You may find that it's difficult to have a complete conversation without you or your spouse leaving the room before the conflict is resolved. The emotional buttons that your spouse pushes in you can make you want to bolt and get away from your uncomfortable feelings and reactions.
Learning to communicate more effectively with your spouse requires that you be fully present and attentive. You have to be committed to really listening and hearing, not only with your ears but also with your heart. You want to eliminate any communication blocks that prevent you and your partner from growing in understanding and intimacy.
Communication blocks are anything that you do, verbally or non-verbally, to keep you from connecting deeply with another person. Some examples of communication blocks in marriage are:
- Rolling your eyes and looking resigned or exasperated when your spouse is talking;
- Sighing deeply and loudly when your spouse is sharing his/her viewpoint;
- Looking at your watch or a clock repeatedly;
- Not stopping what you're doing when your spouse is trying to have a serious talk with you;
- Not making eye contact and not giving your partner your undivided attention;
- Using the time when your spouse is talking to think about other things unrelated to the conversation;
- Tuning your spouse out because you've heard the same thing repeatedly and are convinced it's the same old speech;
- Becoming defensive and angry immediately instead of showing your partner the respect of hearing him/her out;
- Belittling your spouse, name calling, cursing, shaking or pointing a finger, or getting in his/ her face.
- Interrupting your partner before he/she is finished talking.
If you truly love your partner, you will not want to rip him/her to shreds verbally, or to ignore or discount differing opinions and beliefs. You will want to do everything you can to insure that you have quality communication in your relationship and that you are communicating your caring, love, and respect to your spouse.
Eleanor Roosevelt once said, "The most important thing in any relationship is not what you get but what you give." While you cannot control how someone else will react to your efforts, you can commit to doing all you can to create a safe environment where intimacy can flourish.


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|Conventional (equatorial) Mercator map arbitrarily clipped between parallels 85°N and 85°S
The great Flemish cartographer Gerhard Kremer became famous with the Latinized name Gerardus Mercator. A revolutionary invention, the cylindrical projection bearing his name has a remarkable property: any straight line between two points is a loxodrome, or line of constant course on the sphere. In the common equatorial aspect, the Mercator loxodrome bears the same angle from all meridians. In other words, if a straight line is drawn on a Mercator map connecting a journey's starting and ending points, that line's slope yields the journey's unchanging direction; keeping a constant bearing is enough to arrive at the destination.
This projection is almost always presented in a tangent case, with the Equator as a standard parallel free of distortion. When using a secant case, two parallels symmetrically opposite the Equator become standard lines; the resulting map is nearly identical after a change in aspect ratio, much like variations of the equal-area cylindrical projection. In this context, the words "tangent" and "secant" are only conceptual, since the Mercator projection is not defined by a perspective process on a developing cylinder.
|Transverse Mercator map, central meridian 30°W. This projection is not intended for small-scale or whole world maps, as the scale and areal distortion is obvious even moderately far from the central meridian. On the other hand, it is usually the best choice for large-scale precision mapping.
The only conformal cylindrical projection, Mercator's device was a boon to navigators from the 16th-century until the present, despite suffering from extreme area distortion near the poles: in order to keep shapes undistorted, Antarctica is enormously stretched, and Greenland is rendered about nine times larger than actual size. Indeed, stretching grows steadily towards the top and bottom of the map (in the equatorial form, in higher latitudes; the poles would be actually placed infinitely far away). Mercator maps seldom extend above the 80°N parallel or below 75°S. Both this apparent shift of the Equator southwards and the areal exaggeration of intermediate latitudes, which mostly coincide with developed nations, have repeatedly incited disapproval about its supposed bias against the Third World (it was even claimed to aid racial discrimination by promoting a supposed superiority of Europe, the U.S.S.R. or the United States); misguided or naïve controversies and proposals to fix the wrong problem include the "Peters" projection, which (like many others) preserves areas, but strongly distorts shapes and has no especially interesting property to compensate for. Although historically several maps have been enlisted in political propaganda, this is no fault of the projections themselvels: like all conformal projections, Mercator's was never intended for world wall maps. Nevertheless, it was once common in textbooks. More recently, the spherical case was chosen for the world view of Google Maps, clipped between the 85°3'4" latitudes, which yield a square map, convenient for efficient storage and retrieval.
Although fundamental, a Mercator map is not the only one used by navigators, as the loxodrome does not usually coincides with the geodesic, except in short travels. The geodesic may be plotted on a gnomonic map, and later transferred to a Mercator map and split in loxodromes piecewise.
This projection was possibly first used by Etzlaub ca. 1511; however, it was for sure only widely known after Mercator's atlas of 1569. Since a rigorous underlying mathematics was not available at the time, Mercator probably defined the graticule by geometric construction; E.Wright formally presented equations in 1599.
More commonly applied to large-scale maps, the transverse aspect preserves every property of Mercator's projection, but since meridians are not straight lines, it is better suited for large-scale topographic maps than navigation. It does map a single central meridian (two when secant) with no distortion.
|No aspect of the Mercator projection is appropriate for global or small-scale maps. Finding an oblique aspect which shows all major landmasses with no interruptions is a simple exercise for the spherical case — visually interesting but of little practical value; ellipsoidal versions for small regions pose a much more useful and difficult problem.
As usual, equatorial, transverse and oblique versions of Mercator's projection offer exactly the same distortion pattern.
The transverse aspect, with equations for the spherical case, was presented by Lambert in his seminal paper (1772). The ellipsoidal case was developed, among others, by the great mathematician Carl Gauss (ca. 1822) and by Louis Krüger (ca. 1912); it is frequently called the Gauss conformal or Gauss-Krüger projection.
A mathematical challenge, oblique aspects of the Mercator projection in the ellipsoidal case have attracted professional interest for large-scale local or regional maps. Several approaches have been suggested, usually based on intermediate projection surfaces (if successive conformal mappings are applied in sequence, the end result remains conformal) and differing in details like the range of scale distortion; in general the constant scale along parallel lines of the spherical version is not retained.
Jean Laborde's version (1926), applied to Madagascar, first transformed the ellipsoid into a conformal sphere by appropriately shifting parallels, then used an ordinary transverse Mercator projection, followed by a rotation to align Madagascar's longest dimension with what would be the map's central meridian.
Martin Hotine's better-known method — sometimes called the Hotine projection — was first used (ca. 1946) for Southeast Asia, then other areas including in the United States. Instead of a sphere, its intermediate surface is an aposphere, a parametric surface of constant total curvature.
The best known use of the transverse Mercator projection is the specialized form called Universal Transverse Mercator (UTM) projection system.
The UTM defines a grid covering the world between parallels 84°N and 80°S. The grid is divided in sixty narrow zones, each centered on a meridian. Zones are identified by consecutive numbers, increasing from west to east (the first zone, immediately east of the 180° meridian, is numbered 1; zone 31 lies just east of the Greenwich meridian). A set of parallels divides the grid in rows, labeled by letters from C to X (I and O are not used) starting south. Therefore each zone comprises 20 quadrangles, identified by a number-letter pair. Quadrangles are in turn further subdivided in squares 100 km-wide, identified by double letter combinations.
|Although no Mercator map is created by a perspective process, the cylinder is a useful visualization aid. The blue strip is zone 13 of the UTM grid; it is part of a cylindrical slice, approximating a spherical lune 6° wide at the equator and clipped by the 84°N and 80°S parallels. This is not the shape of UTM maps; rather, each UTM zone is covered by many partly juxtaposed rectangular large-scale sheets.
Each zone is separately projected using the ellipsoidal form of the transverse Mercator projection with a secant case: scale of the central meridian is reduced by 0.04%, so two lines about 1°37" east and west of it have true scale. The UTM grid was designed for large-scale topographic mapping in separate sheets, not for world or regional maps. In particular, sheets from different zones don't juxtapose exactly.
Within each quadrangle, any point may be located by two distances in meters: the easting, east from the central meridian and the northing, north from the Equator. The central meridian's coordinate is always 500,000; the Equator's coordinate is designated 0 for quadrangles in the northern hemisphere, and 10,000,000 for quadrangles in the southern hemisphere. Since the distance from poles to Equator is approximately 10,000 km, such offset origins ensure coordinates (the false eastings and false northings) are always positive.
The UTM grid is fairly regular, with a few exceptions:
The original UTM system was adopted by the U.S. Army in 1949, and variations afterwards by several agencies throughout the world. Despite the name, it is not actually "universal" in the sense that each grid may be based on a different datum, so sheets from different grid sets may or may not be compatible. UTM maps are of course conformal, and distance and area distortion are limited by the large scale of individual sheets.
The azimuthal equidistant projection is trivially easy to draw in the polar aspect and, like all azimuthal designs, it features some special properties for the central point alone: all straight lines touching it are geodesics, and the angle between any two of those lines is the same as on Earth. Hence, oblique azimuthal equidistant maps must be tailor-made for each specific location.
The projection's modern name is due to Antonio Cagnoli, who reinvented it in 1799; earlier it had been mentioned by J.Lambert in his seminal paper of 1772, Guillaume Postel (1581), who is often credited as the original author, and Glareanus (ca. 1510), among others.
As a rule, azimuthal projections make straightforward finding true directions from a single point (the reference straight line is, of course, the local meridian): a short wave radio operator whose hardware is stationed at the map's center can use it in order to orient its antenna for maximum gain towards anywhere on Earth.
Furthermore, since of all azimuthal projections the equidistant alone preserves radial distances from the central point, the operator may estimate how much power is required for ensuring stable communication. Likewise, the captain of a nuclear submarine could use this projection to check which cities lie inside its destructive range. Other projections simply are not appropriate.


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Hi there dear Dreamstimers
I am writing from an hotel room in Seville in Spain and once i came back from my shooting day in this BEAUTIFUL town, i found out that the first picture i uploaded on this very site, just got level 3 with its 10th DL. :)
And wanted to celebrate with you all.
Have a good week tomorrow
Antonio Jodice
Firenze, IT
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Auto Source Service Center
ReScore Reviews™ (2)
99% Would Recommend
Latest Review 3 days ago
Review for Auto Source Service Center
Lucy L. on 07/23/2015
Lucy said she has been going to this business for several years. They always take good care of her vehicle. She is very happy with their service. They have good chats and share some goodies.
Vehicle: Mercury Grand Marquis
Category: Service
Service Date: 07/21/2015
Review Created: 07/23/2015
This review was collected via phone interview by Customer Research Inc.


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- Please select a date to see the proper prices!
- Meal can be requested on the spot
Best offer
50 € / person / night, no meal
Description of the accommodation:
Rooms, services Guests looking for accommodation in Vienna are welcome to book 27 nicely furnished rooms at Hotel-Pension Bleckmann Vienna. Should you be arriving by car, parking will be available nearby. Car rental service and organized city tours can also help you discover all important attractions nearby. You can also bring your laptop for the holiday, for there is wireless Internet connection (WiFi) available at the pension. You can find the exact list of services provided by Hotel-Pension Bleckmann Vienna (including those for extra fees) and the exact equipment of the rooms on the information sheet below.
Payment In order to finalize the reservation you may be required to provide credit card guarantee or advance payment by credit card, depending on the chosen room, date and price.
Arrival, opening hours The earliest arrival time is 14:00, the latest time to leave the rooms is 11:00. English and German languages are spoken by the personnel.
Hotel-Pension Bleckmann Vienna rooms Rooms
- Please select a date to see the proper prices!
Accommodation services
Parking (18 €/night)
Free WiFi in shared places
Organized sightseeing nearby, Car rental nearby
Pet friendly (15 €/night), Elevator
Hairdryer, Desk in the room


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So about that resolution? I confess, I didn't make them this year. I never make them, at least not for New Years. I am one of those weird ones that think your birthday is the start of your New Year and if you're going to make resolutions you should do it then.
But let's face it, my last two birthdays have sucked mule eggs ( HAD to use that one, a Belle's grandmother said it and it cracked me up).
On my birthday in 2015 my niece Caylee was murdered. To say I didn't have sense enough to even think of anything other than not losing my mind would be an understatement. I've decided not to celebrate my birthday on that date and if I could legally change the date of my birth I would. Probably not what Caylee would want me to do, but there is nothing about that day that will ever make me want to celebrate anything.
If you follow this page at all, then you know on my last birthday (2016) I was sitting in SICU at Duke University while fighting for my husband's life. Rather he was fighting and I was sending him every ounce of energy I had.
The ONLY resolution I've made for the last two years is NOT to give up. Just keep hanging on and putting one foot in front of the other and remember to breathe once in a while. That's all I've been able to do. I'm not sure if it even qualifies as a resolution. It's just the only way I know how to handle the hand I've been dealt.
Did you make any resolutions for the New Year?
Welcome to a Secret Subject Swap. This week 14 brave bloggers picked a secret subject for someone else and were assigned a secret subject to interpret in their own style. Today we are all simultaneously divulging our topics and submitting our posts.
My prompt was:
So . . . about that resolution . . .
It was submitted by: http://notthatsarahmichelle.blogspot.com
Sit back, relax and check out what everyone else is writing about this week.
The Diary of an Alzheimer's Caregiver
Confessions of a part time working mom
COPYRIGHT© 2017– Southern Belle Charm~ Unauthorized use of this material without express written consent from this blog's author's and/or owner's is strictly prohibited. Excerpts and links may be used, provided full credit is given to Southern Belle Charm and/or it's owners with appropriate and specific direction to the original content.


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"11M G.fast subs/year in 2021 predicted by nbn/Ovum" was my original headline. After reading the report, I saw no way to be so precise five years from now. There are too many unknowns. Ovum sees only 330,000 in 2017. My guess for 2017 would be higher. Technicolor has an order in hand for 100,000 modems, probably used in VDSL mode until G.fast is ready. AT&T is passing 3M homes a year with fiber and/or G.fast. They are being coy about G.fast but see large savings likely. BT is passing 140K homes in the first half of the year, then ramping rapidly to 3M/year.
SK in Korea will be in a race with Korea Telecom for the 9M homes in the country that need upgrades. KT is promising to offer G.hn at hundreds of megabits to 95% of the 9M by the end of 2017; SK can't afford not to move quickly. I've 12 countries on the G.fast map with firm deployments. Over 60 more are in trials.
Most of the planned builds will still be ramping late in 2017. A modest delay could easily push subscriber adds into 2018. Data for 2019-2021 are highly speculative.
Broadcom and Sckipio, the leading chipmakers, have incorporated important advances in their chips. Sckipio customers have confirmed to me DTA, 30A, and the fiber extender are working well and are comfortable moving ahead. I haven't yet heard from Broadcom customers so have no way to estimate when their new features will be ready. They don't even have a product sheet for me, much less a reference customer or field trial data.
I have the Ovum data from Petroc Wilson of Commsday. "In 2021 Ovum expects G.fast to be supporting nearly 29 million subscribers, representing 3% of the global fixed broadband market. As an emerging technology the growth in annual subscriber additions is expected to accelerate in each year, rising from 330,000 in 2017 to nearly 11.5 million in 2021." Mistakes in the rest of the article are mine. Petroc is one of the few reporters I'm willing to pick up without checking quotes. He and COMMSDay colleagues are exceptional. Their newsletter requires a professionally priced subscription but is read by everyone interested in Australia and New Zealand telecom. Update 10/17 - the report is out and the summary below.
I'm 12,000 miles away so can't be sure of the reason nbn is placing cabinets close to homes (FTTdp) and then filling them with second-rate VDSL DSLAMs. It's an obvious mistake. Everything about the nbn is very political. My best guess is that they are looking for the government to increase their subsidies and are holding back on G.fast for political reasons.
G.Fast set for nearly 30 million end users by 2021
17 October 2016
New nbn-BT report 'Gigabit Networks' predicts bright future for G.Fast and XG.Fast
A new Ovum report jointly commissioned by nbn and BT has predicted that new ultra-fast copper technology G.fast will be serving nearly 30 million subscriber homes and businesses around the world by 2021. This is just four years after the first commercial G.fast services are due to launch in 2017.
"In 2021, Ovum expects G.fast to be supporting nearly 29 million subscribers, representing 3% of the global fixed broadband market," the Gigabit Networks report says.
"As an emerging technology, the growth in new G.fast subscribers is expected to accelerate in each year, rising from 330,000 in 2017 to nearly 11.5 million in 2021."
nbn held its first G.fast trial in October 2015 and has been conducting additional lab trials of XG.FAST in recent weeks.
nbn recently announced its intention to deploy Fibre-to-the-Curb (FTTC) technology to approximately 700,000 premises – giving an ideal platform for potentially deploying both future G.fast and XG.FAST services.
Ovum is forecasting particularly strong take-up of G.fast in Europe, with operators including BT, Swisscom, Deutsche Telekom, Telekom Austria and Proximus (Belgium) all planning to deploy G.fast technology to deliver affordable ultrafast connectivity quickly and cost-effectively.
"About 11% of broadband services in Western Europe may be delivered via G.fast within five years, with a number of major markets already including the technology in their upgrade plans," the Gigabit Networks report says.
Openreach, BT's local network division, is expected to be one of the first operators in the world to launch commercial G.fast services with a launch expected during 2017.
It is aiming to make ultrafast speeds available to 12 million premises in the UK by the end of 2020 – using a mix of G.fast and Fibre to the Premises (FTTP) technologies.
Openreach is already working to conduct the largest G.fast field trials in the world, delivering speeds of up to 330Mbps. The company recently announced an extension to its pilot which will see around 140,000 homes and businesses across the country given access to G.fast by March 2017.
Gigabit Networks points out that XG.FAST will enable service providers to take DSL-based broadband to an entirely new level.
"Developed by Nokia Bell Labs, XG-FAST is targeting throughput of up to 10Gbps over very short bonded copper lines," the report says.
"This effectively positions XG-FAST as a fibre extension solution that avoids the cost, and often logistical challenges, of accessing the premises."
Dennis Steiger, nbn's Chief Technology Officer, said:
"This report shows the potential that G.fast has for delivering ultra-fast broadband services in the global market.
"It is very challenging to deliver fibre into every home. Having the option to use G.fast in a Fibre-to-the-Building or Fibre-to-the-Curb setting is a great option for any operator.
"With G.fast able to deliver fibre-like speeds at a lower cost and time to deploy, it's little surprise the technology is attracting strong global interest," said Steiger.
Clive Selley, CEO of Openreach, said:
"Our aim is to make ultrafast broadband available to 12 million homes and businesses in the UK by the end of 2020, and we're embracing a mix of technologies with G.fast and FTTP to achieve that.
"We have pioneered G.fast in our labs, driven the global standards, and have been working closely with our communications provider customers on the trials, so we're very excited that it's time to start rolling this technology out nationwide.
"The great thing about G.fast is that allows us to deliver affordable ultrafast speeds to customers quickly and at scale."
Matthew Howett, Practice Leader, Regulation & Policy, Ovum said:
"G.fast is a progressive and logical step for any network operator looking to deliver ultrafast speeds through incremental enhancements to existing infrastructure. It allows them to radically improve the available speeds for large numbers of subscribers in a much shorter timeframe than other fibre based solutions."
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Anti-capitalist activists have occupied an empty office block in London owned by Swiss banking giant UBS, their third protest site in the British capital, the movement said Friday.
The Occupy London Stock Exchange movement said a dozen demonstrators gained access to the building in the Hackney area of east London overnight and secured it, adding that they planned to use it as a "bank of ideas."
The London protesters, inspired by the Occupy Wall Street demonstrators in New York, also have camps outside St Paul's Cathedral in the heart of the fiancial district and in Finsbury Square, also near major banks.
"As banks repossess families' homes, empty bank property needs to be repossessed by the public," Occupy LSX member Jack Holburn said in a statement on the movement's website.
"We hope this is the first in a wave of public repossessions of property belonging to the companies that crashed the global economy."
UBS said it was taking action, amid reports that security guards had turned up outside the building.
"We are aware of the situation and are taking appropriate action," it said in a statement.
London's Metropolitan Police had no immediate comment.
On Thursday night the protesters camped outside historic St Paul's refused to leave despite the expiry of a deadline issued by the Corporation of London, the local authority for the financial district.
The authority has said it will now take court action to evict the protesters, who have been camped outside the church for more than one month.


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- "At least a hundred little elves were standing around the kitchen, beaming, bowing, and curtsying as Dobby led Harry past them. They were all wearing the same uniform: a tea towel stamped with the Hogwarts crest, and tied, as Winky's had been, like a toga."
- —Description[src]
These tea towels were originally used to dry china, although they ended up becoming the serviceable uniform of the Hogwarts house-elves, who often wore it tied like a toga.[1]
- Harry Potter and the Goblet of Fire (First appearance)
- Pottermore
Notes and references
- ↑ Harry Potter and the Goblet of Fire, Chapter 21 (The House-Elf Liberation Front)


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Gehwol Classic Leg Balm 4.22oz
Product DescriptionGehwol Classic Leg Balm, with daily application, helps rough skin become soft, smooth, improves the appearance of the skin and prevents dry scaly skin. Leg Balm is mosturizing, and is quickly absorbed and non-greasy. Gehwol Classic Leg Balm deodorizes and soothes irritation while tightening your skin. 4.22oz
Suitable for Diabetics. For dry or prematurely aged skin and varicose veins.
Allantoin and bisabolene, active ingredients of horse chestnut and chamomile, as well as panthenol have a soothing effect on reddening and uncleanness of the skin. Together with extracts from the Virginian witch hazelnut bush (hamamelis), it has an astringent effect and relieves irritation.


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Kaibutsu-kun: The Movie Trailer (2011)
26 November 2011 Fantasy, Comedy 103 mins
Kaibutsukun is the young prince of Kaibutsu land who decides to go to Earth to prove to his people that he is worthy of becoming king. Kaibutsukun is accompanied by his friends Dracula, Wolfman, and Franken. The Prince decides to go to Japan to visit his friends Utako and Hiroshi. The Prince, Dracula, Wolfman and Franken are in for a surprise.
Watch the official Kaibutsu-kun: The Movie 2011 trailer in HD below or find more Kaibutsu-kun: The Movie videos on Vidimovie.


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Fernando's Win-RAID Forum (Storage Drivers - BIOS Modding) » Important Drivers (AHCI/RAID, NVMe, USB etc.) » Specific: Intel AHCI/RAID Drivers » Intermittent startup freeze due to Intel AHCI driver
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General & Forum related Topics News / Announcements Questions/Suggestions regarding the Forum Off Topic Important Drivers (AHCI/RAID, NVMe, USB etc.) General: Storage Drivers (AHCI/RAID, NVMe and USB) Specific: Intel AHCI/RAID Drivers Specific: NVIDIA nForce Chipset Drivers (incl. AHCI/RAID) Specific: NVMe Drivers (incl. NVMe Support without BIOS Modding) Other Drivers BIOS Modding BIOS Modding Guides and Problems BIOS modules (PCI ROM, EFI and others) BIOS Modding Results Offer: Already modded special BIOSes System Performance RAID Performance AHCI/NVMe Performance Solid State Drives Special Topics Special: Intel Management Engine Special Tools (not BIOS modding related) Special: Win7/Win8/Win10 Special: Windows XP/W2k/W2k3
#1 | Intermittent startup freeze due to Intel AHCI driver Sat Nov 19, 2016 7:59 pm
#2 | RE: Intermittent startup freeze due to Intel AHCI driver Tue Nov 22, 2016 9:01 pm
How to enable AHCI/RAID on x79 in Windows XP 32-bit? » « Confused on which driver to use for laptop chipset
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100% garanti
"If we want things to stay as they are, things will have to change." This sentence from the movie The Leopard was written by Giuseppe Tomasi di Lampedusa. It is representative of the Italian political system which has been in place since the post-1945. In fact, Italy is characterized by a multi-party system with two largest parties. During the 90's the party system experienced a change of the "party landscape" and a new electoral system. I will try to show that despite these changes, the party system has not really been modified.
Despite some changes, the party system has not really been modified.
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Phone: 561 790-1200
Fax: 561-798-0774
600 Sansbury's Way
West Palm Beach, FL 33411
About Manheim Palm Beach
Sale Days
Weekly Consignment Sale Every Thursday, 1500+ units at 9am
Exotic Highline Sale Every four weeks on Wednesday at 9am
Manheim OVE Mondays Weekly sale on OVE.com. Absolute Auction. Pre-sales are listed starting at 8pm every Friday, bidding ends at 2pm ET on Monday.
Tuesday Online Event Sale Every Tuesday from 2-4pm ET. Featuring Mercedes-Benz Financial Services on OVE.com. Pre-sales listed every weekend. Absolute Auction.
Hours of Operation
Main Office
Mon-Thu 9am-5pm
Fri 9am-3pm
Transport/Gate Hours
Mon-Fri 9am–7pm
Sat 10am-7pm
Vehicle Pickup
Sale days 6am-12m
Other days 6am-10pm
Vehicle Drop Off
24 hours, 7 days a week
If the drop off is taking place after normal hours, our Security team will contact the Manager on-call to print missing gate passes.
Sales and Promotions
List of Approved Highline Makes
Manheim Palm Beach is pleased to announce the start of a second sale that will be running Tuesdays at 2pm starting on Jul 19.
Thursday Thunder All powersports units including Jet Skis, Motorcycles, and Boats will be running at 8:30am on Thursdays in Lane 9.
Napleton has amped up their weekly drawing to a continuous progressive pot. At the end of their sale, they're going to draw one random buyer to win. The buyer must be present to win. If the buyer is not present, the progressive pot will carry over to the next week, and continue to increase at the next sale. They are running every Thursday in Lane 4 at 9:15am with 80+ units.
Nissan will now be running 30+ units weekly in Lane 1 at 9:15am on Thursdays. Join us for some of South Florida's front line ready units.
We have special rates with most of the hotels in our area. Please email reservation requests to Shannon Alaimo or feel free to book your own room.
Local Policies
Manheim Cargaritaville 2017 Coupon Promotion Terms and Conditions
Manheim Palm Beach Off Lease Only Inc. June 8 Progressive Jackpot Sweepstakes Official Rules
Manheim Palm Beach Get a Hole in One with Earl Stewart! Sweepstakes Sweepstakes Official Rules
Manheim Palm Beach Porsche West Broward Cruise Around the Block! Sweepstakes Official Rules