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Conceived and designed the experiments: RG SK JGV. Performed the experiments: CRT RG NC DR RJ FO AS DEE. Analyzed the data: CRT RG SK JGV. Contributed reagents/materials/analysis tools: NC DR RJ FO AS DEE AC APdS CIB CIdO IM CHNC PV AB. Wrote the paper: CRT RG SK JGV.
Sand flies deliver Leishmania parasites to a host alongside salivary molecules that affect infection outcomes. Though some proteins are immunogenic and have potential as markers of vector exposure, their identity and vector specificity remain elusive.
We screened human, dog, and fox sera from endemic areas of visceral leishmaniasis to identify potential markers of specific exposure to saliva of Lutzomyia longipalpis. Human and dog sera were further tested against additional sand fly species. Recombinant proteins of nine transcripts encoding secreted salivary molecules of Lu. longipalpis were produced, purified, and tested for antigenicity and specificity. Use of recombinant proteins corresponding to immunogenic molecules in Lu. longipalpis saliva identified LJM17 and LJM11 as potential markers of exposure. LJM17 was recognized by human, dog, and fox sera; LJM11 by humans and dogs. Notably, LJM17 and LJM11 were specifically recognized by humans exposed to Lu. longipalpis but not by individuals exposed to Lu. intermedia.
Salivary recombinant proteins are of value as markers of vector exposure. In humans, LJM17 and LJM11 emerged as potential markers of specific exposure to Lu. longipalpis, the vector of Leishmania infantum chagasi in Latin America. In dogs, LJM17, LJM11, LJL13, LJL23, and LJL143 emerged as potential markers of sand fly exposure. Testing these recombinant proteins in large scale studies will validate their usefulness as specific markers of Lu. longipalpis exposure in humans and of sand fly exposure in dogs.
Leishmania parasites are transmitted by the bite of an infected vector sand fly that injects salivary molecules into the host skin during feeding. Certain salivary molecules can produce antibodies and can be used as an indicator of exposure to a vector sand fly and potentially the disease it transmits. Here we identified potential markers of specific exposure to the sand fly Lutzomyia longipalpis, the vector of visceral leishmaniasis in Latin America. Initially, we determined which of the salivary proteins produce antibodies in humans, dogs, and foxes from areas endemic for the disease. To identify potential specific markers of vector exposure, we produced nine different recombinant salivary proteins from Lu. longipalpis and tested for their recognition by individuals exposed to another human-biting sand fly, Lu. intermedia, that transmits cutaneous leishmaniasis and commonly occurs in the same endemic areas as Lu. longipalpis. Two of the nine salivary proteins were recognized only by humans exposed to Lu. longipalpis, suggesting they are immunogenic proteins and may be useful in epidemiological studies. The identification of specific salivary proteins as potential markers of exposure to vector sand flies will increase our understanding of vector–human interaction, bring new insights to vector control, and in some instances act as an indicator for risk of acquiring disease.
Sand fly salivary proteins play a major role in blood feeding and Leishmania transmission [1]–[3]. Exposure to sand fly salivary proteins induces both cellular immunity and specific antibodies [3],[4]. A relationship between the level of specific antibodies to saliva, vector exposure and risk of contracting disease has been demonstrated for different vector-host models [5]–[9]. Production of antibodies against mosquito and tick saliva not only contributed to development of host allergic reactions but was strongly related to risk of disease development [5],[10]. Similarly, in an endemic area of Senegal, production of antibodies against Anopheles gambiae salivary proteins was identified as an indicator of the risk of malaria [10]. This correlation was also observed for tick exposure, where antibody production against tick saliva was associated with self-reported tick exposure and Lyme disease [11]. Recently, saliva of Triatoma infestans was shown to be a potential marker for vector infestation in domestic animals [12]. Therefore, the detection of antibodies against the saliva of hematophagous insect vectors could be used as an indicator of vector exposure and in some instances as an indicator for risk of contracting disease.
Previous work shows that humans and animals exposed to sand fly bites or immunized with saliva can develop antibodies that recognize specific salivary proteins [4], [7], [13]–[15]. In São Luis, an area of endemic visceral leishmaniasis (VL) in Maranhão, Brazil, the presence of anti-saliva antibodies in humans strongly correlated with protection and the development of anti-Leishmania delayed-type hypersensitivity response [7]. Furthermore, individuals that poorly recognized salivary proteins developed anti-Leishmania antibodies associated with disease progression [7]. In contrast, in areas endemic for cutaneous leishmaniasis (CL)—such as Canoa (Bahia, Brazil) and Sanliurfa (Turkey)—the presence of anti-saliva antibodies correlated with risk of contracting disease [16],[17].
The presence of antibodies to sand fly salivary proteins has also been demonstrated in animal reservoirs of leishmaniasis. In canines, two sand fly salivary proteins were recognized by sera of infected dogs from an endemic VL area in Brazil [18]. Hostomska et al. [14] reported the presence of anti-saliva antibodies to six different sand fly proteins in dogs experimentally exposed to Lutzomyia longipalpis bites. Importantly, foxes captured in Teresina, an endemic VL area in Brazil, also showed high levels of anti-saliva antibodies, particularly to a 44-kDa salivary protein from Lu. longipalpis, suggesting exposure to bites of this vector [19]. Hence, vector salivary proteins also represent a potential tool as markers of exposure to important reservoirs of disease.
Identification of the sand fly salivary proteins recognized by the mammalian host will not only increase our understanding of vector-host interactions but will also aid in developing new epidemiological tools to correlate host exposure to vector sand flies with immunity or susceptibility to leishmaniasis. It will also help identify potential reservoirs of Leishmania. Here we describe a practical functional transcriptomic approach for the identification of the Lu. longipalpis salivary proteins most recognized by humans and canids (dogs and foxes) using sera from São Luis and Teresina, endemic areas for VL in Brazil [15],[20].
Lu. longipalpis (Jacobina strain) were reared at LMVR, NIAID, USA; Lu. verrucarum (Peru strain) and Phlebotomus perniciosus (Italy strain) at WRAIR, USA; Lu. intermedia (Corte de Pedra strain) were obtained from CPqGM (FIOCRUZ, Bahia, Brazil). Females were used for dissection of salivary glands 5–8 days post-eclosion; SGH was prepared as described elsewhere [21]. Briefly, salivary glands were dissected and stored in sterile PBS (pH 7.4) at −70°C. To obtain the homogenate, salivary glands were disrupted by ultrasonication and the supernatant collected after centrifugation at 15,000g for 2 minutes.
A total of 14 human sera from from a VL-endemic region in São Luis (Maranhão, Brazil) [15] and 6 from a CL-endemic region in Canoa (Bahia, Brazil) [22] were used in this study. Informed written consent was obtained from parents or legal guardians of minors. The project was approved by the institutional review board from the Federal University of Bahia (1993) and the Federal University of Maranhao (1996). Dog and fox (Cerdocyon thous) sera (total of 8 and 11, respectively) were from animals captured in a VL-endemic area around Teresina (Piaui, Brazil) [19]. Fox and dog studies were approved in 2000 by the Brazilian agency for protection of the wildlife (IBAMA/PI) and in 2005 by the Federal University of Piaui. Sera were also obtained from dogs (total of 6) experimentally exposed to Lu. longipalpis [14]. Dog studies were approved by Bayer Health Care AG (Leverkusen, Germany) and handled in accordance with the European guidelines for animal husbandry.
DNA was amplified by polymerase chain reaction (PCR) using a forward primer deduced from the amino-terminus and a reverse primer encoding a hexhistidine motif. PCR amplification conditions were: one hold of 94°C 5 min, two cycles of 94°C 30 s, 48°C 1 min, 72°C 1 min, 23 cycles of 94°C 30 s, 58°C 1 min, 72°C 1 min, and one hold of 72°C 7 min. The PCR product was cloned into the VR2001-TOPO vector and sequenced [23].
A plasmid encoding a distinct salivary protein (1 µg/µl) was injected intradermally into female Swiss Webster mice in 10 µl, three times at two-week intervals to generate polyclonal antibodies for each of the nine selected candidates [23].
Recombinant proteins were produced by transfecting 293-F cells (Invitrogen) with plasmid following the manufacturer's recommendations. After 72 h, the supernatant was recovered, filtered and concentrated to 30 ml in an Amicon concentrator device (Millipore) in the presence of Buffer A (20 mM NaH2PO4, 20 mM Na2HPO4, pH 7.4, 500 mM NaCl). A HiTrap chelating HP column (GE Healthcare) was charged with 5 ml of 0.1M Ni2SO4. The concentrated protein was added to the HiTrap chelating HP column that was then connected to a Summit station HPLC system (Dionex, Sunnyvale, CA) consisting of a P680 HPLC pump and a PDA-100 detector. The column was equilibrated for 30 min with Buffer A at 1 ml/min. Elution conditions were: 0-5 min, 100% Buffer A; 5-15 min, a gradient of 0% to 100% Buffer B ( Buffer A+50 mM imidazole); 15-20 min, a gradient of 0% C (Buffer A+500 mM imidazole) to 10% C (90% B); 20-25 min, 90% B and 10% C; min 25-30, a gradient of 10% C to 20% C (80% B); 30-35 min, 80% B and 20% C; 35-40 min, a gradient of 20% C to 100%C; and 40-50 min, 100% C. Eluted proteins were detected at 280 nm and collected every minute on a 96-well microtiter plate using a Foxy 200 fraction collector (Teledyne ISCO).
Five-microliter aliquots of all fractions were blotted on nitrocellulose and blocked with TBS-tween 3% non-fat milk for 1 h and then incubated for 1 h with anti-saliva antibodies, washed, and incubated for 1 h with an anti-mouse IgG (H+L) alkaline phosphatase-conjugated secondary antibody (Promega). Positive fractions were developed with Western Blue stabilized substrate for alkaline phosphatase (Promega). Positive fractions were run on sodium dodecyl sulfate (SDS-PAGE) and silver stained using SilverQuest (Invitrogen). Imidazole was removed from positive fractions by dialysis overnight against PBS, pH 7.4.
Salivary glands (40 pairs approximately equivalent to 40 µg total protein) or soluble recombinant sand fly salivary proteins (20 µg) were run on a 4–20% Tris-glycine gel or on a 4–12% NuPAGE gel. After transfer to a nitrocellulose membrane using the iBlot device (Invitrogen), the membrane was blocked with 3% (w/v) nonfat dry milk in Tris-buffered saline (TBS)-0.05% Tween, pH 8.0, overnight at 4°C. After washing with TBS-T, pH 8.0, the membrane was placed on a mini-protean II multiscreen apparatus (Bio-Rad, Hercules, CA), and different lanes were incubated with various sera (180 dilution, human and dog sera; 150 dilution, fox sera) for 3 h at room temperature. After washing with TBS-T, pH 8.0, three times for 5 min, the membrane was incubated with either anti-dog IgG (H+L) alkaline phosphate-conjugated antibody (110,000) (Jackson Immuno Research) for 1 h at room temperature for dog and fox sera or with anti-human IgG alkaline phosphate-conjugated antibody (18,000) (Sigma) for human sera. Membranes were developed by addition of Western Blue stabilized substrate for alkaline phosphatase (Promega), and the reaction was stopped by washing the membrane with deionized water.
Lu. longipalpis salivary glands contain a large number of secreted proteins (figure 1A). Fourteen human sera from individuals living in São Luis, an area where Lu. longipalpis predominates, recognized a considerable number of these proteins, mainly between 15 and 65 kDa (figure 1B). Eight dog sera from Teresina recognized a large number of salivary proteins, many of the same size as human sera as well as several proteins of different sizes (figure 1B). As for foxes, 11 sera collected in the same endemic area as dogs recognized only a few salivary proteins and only one strongly of approximately 50 kDa (figure 1B).
To determine the specificity of human and dog sera for Lu. longipalpis salivary proteins, we tested the most reactive human (two) and dog (one) sera against salivary proteins from other sand fly species including Lu. intermedia which transmits CL in South America [24], Lu. verrucarum which transmits CL in Central and South America [25], and Phlebotomus perniciosus which transmits VL in Mediterranean countries [26]. Human sera recognized multiple bands of Lu. longipalpis saliva (figure 2A). One of the 2 human sera also recognized two salivary proteins from Lu. intermedia (figure 2A). We cannot exclude the possibility that this individual was weakly exposed to Lu. intermedia bites, as this species is also present, albeit rare, in São Luis [20] or to other non-abundant species in the area. All tested sera recognized proteins between 28 and 50 kDa from Lu. verrucarum saliva and a protein of approximately 40 kDa from P. perniciosus saliva (figure 2A); both species do not overlap with Lu. longipalpis in their geographical distribution.
Given that Lu. longipalpis and Lu. intermedia are sympatric in several areas of Brazil [27] we decided to further investigate the possibility of antibody cross-reactivity between these two species. To address this, we tested six human sera from Canoa (an area in Brazil endemic for CL where Lu. intermedia predominates) against Lu. longipalpis saliva. These sera did not recognize any of the salivary proteins of Lu. longipalpis but recognized those of Lu. intermedia (figure 2B). Together, these results suggest an overall low level of cross-reactivity between Lu. longipalpis and Lu. intermedia salivary proteins.
Because in an endemic area there is no control of the diversity and intensity of exposure of hosts to sand fly bites—both of which can influence antibody response [14]—we compared dogs from Teresina, where Lu. longipalpis is prevalent, with dogs experimentally exposed to Lu. longipalpis bites. Overall, the reactivity of sera from experimentally exposed dogs was considerably lower than that of dogs from Teresina. Both dogs from Teresina and experimentally exposed dogs recognized proteins between 15 to 65 kDa (figure 3). Both groups recognized multiple proteins in Lu. longipalpis saliva but also a few in the saliva of Lu. verrucarum and P. perniciosus. This, together with results from human sera, suggests that antibodies against these proteins may be cross reactive for these two species. Additionally, while proteins from 28 to 50 kDa from Lu. intermedia were recognized by sera of dogs from Teresina, only one protein was poorly recognized by sera from experimentally exposed dogs. Sera from foxes were also tested but showed no cross-reactivity with the other species (data not shown).
Nine abundant transcripts corresponding to the predicted molecular weight of the most antigenic salivary proteins recognized by human, dog, and fox sera within the range of 15 to 65 kDa (figure 1) were selected for expression (Table 1). Figure 4 shows a flow diagram of the approach used to express and purify the nine chosen recombinant salivary proteins. Notably, the same DNA plasmid is used for recombinant protein expression and antibody production. Nine different salivary proteins were expressed and a high level of purification was achieved by HPLC. Purification of recombinant salivary protein LJM17 resulted in a well separated peak eluting at 35–40 min (figure 4B). Aliquots of eluted fractions were recognized by sera of mice immunized with LJM17 DNA plasmid (figure 4C). SDS-PAGE of positive fractions shows a single band of approximately 50 kDa (figure 4D), the expected size predicted by the LJM17 transcript. Similar results were obtained with the other eight expressed proteins: LJM111, LJM11, LJL143, LJL13, LJL23, LJM04, LJL138, and LJL11 (data not shown).
To determine whether the nine expressed salivary recombinant proteins were recognized by sera from humans, dogs, and foxes, we chose those that recognized a considerable number of proteins (from total sand fly saliva) with some degree of variability for further analysis by western blot. Of the nine recombinant proteins tested, LJM17, a yellow-related protein of 45 kDa, was the only protein recognized by sera from the 3 different hosts (figure 5; data for foxes not shown). LJM11, a 43-kDa protein also of the yellow family of proteins, was recognized by human and dog sera, while a third yellow-related protein, LJM111 (43 kDa) was only recognized by human sera (figure 5). LJL23, LJL13, and LJM04 proteins were recognized only by dog sera; LJL143 was recognized by dog sera and weakly recognized by human sera (figure 5). LJL11 and LJL138 were not recognized by any of the sera tested (data not shown).
To confirm the specificity of LJM17 and LJM11 as potential markers of Lu. longipalpis exposure, we tested human sera from São Luis and Canoa where Lu. longipalpis and Lu. intermedia predominate, respectively. Both LJM17 and LJM11 were recognized specifically by human sera from São Luis but not from Canoa (figure 6).
Among parasitic diseases, leishmaniasis has one of the most complex epidemiologies. There are numerous Leishmania species and some cause a wide range of clinical manifestations and involve a large number of proven and potential reservoir hosts. In most cases, each form of leishmaniasis is transmitted by a sand fly species that acts as a principal vector; a few Leishmania species have multiple vector species [28]. In addition, endemic areas of leishmaniasis support several sand fly species other than the vector species responsible for Leishmania transmission. Finding tools to measure exposure of humans and reservoir hosts to specific vectors would provide valuable information regarding their contribution to parasite transmission and would be useful for assessing the risk of contracting disease.
Several studies have demonstrated that anti-saliva antibodies can be used to assess exposure of humans and other Leishmania hosts to sand fly bites and suggested that sand fly salivary proteins represent attractive targets for development of specific markers of vector exposure [4],[14],[16]. To date, none of the salivary proteins of sand flies have been characterized for their immunogenicity and specificity in mammalian hosts, an important prerequisite for their reliability as markers of exposure.
In the present work, we developed a robust method for producing and purifying recombinant salivary proteins. This approach proved highly successful in obtaining pure recombinant proteins that retain recognition epitopes. The purity of the produced recombinant salivary proteins is demonstrated by the presence of a single protein band following SDS-PAGE and silver staining; this level of purity was obtained for all recombinant salivary proteins tested. Soluble and pure recombinant proteins are likely to be properly folded and to better resemble native proteins. This should improve the sensitivity of the detection, enhancing recognition of such proteins by test sera and increasing the specificity of a test by decreasing the chances of false negatives often caused by impurities in the preparation. It is worth noting that the tested salivary recombinant proteins demonstrated specific responses, as two (LJL11 and LJL138) of the nine proteins were not recognized by any of the tested sera.
The seven immunogenic recombinant salivary proteins were differentially recognized by human, dog, and fox sera, the three host species investigated. Some proteins displayed host-specific recognition, reinforcing the importance of testing potential salivary markers for exposure against a variety of hosts to determine their range of applicability. Recombinant proteins LJM17 and LJM11 were strongly recognized by human sera from São Luis, a VL endemic area where Lu. longipalpis predominates, representing 66.4% of captured sand flies [29]. Notably, human sera obtained from Canoa, a CL area where Lu. intermedia represents 94% of the sand fly population [22], did not recognize LJM17 or LJM11. Lu. longipalpis and Lu. intermedia are sympatric species in many endemic areas of Brazil, often representing the two most abundant sand fly species [27]. As such, LJM17 and LJM11 can be considered as potential specific markers of exposure to Lu. longipalpis in areas where other man-biting sand fly species are present in negligible numbers [20],[24],[27]. LJM17 and LJM11 should be tested for specificity against other man-biting sand fly species that are relatively abundant in endemic areas—such as Lutzomyia whitmani [29]—to expand their utility as specific markers of exposure to Lu. longipalpis. Indeed, the faint bands recognized in Lu. intermedia saliva against one reactive human serum from São Luis (figure 2) may have been due to cross reactivity between salivary proteins of this species and those of Lu. whitmani, reported to constitute as much as 24% of the sand fly population in this area [29]. The absolute specificity of LJM17 and LJM11 for Lu. longipalpis exposure therefore requires further confirmation through studies that target more sand fly species. Serum samples from São Luis also recognized salivary proteins from P. perniciosus, a vector of VL in the Old World [30]. This is interesting, as sera from inhabitants of Sanliurfa, Turkey, where P. papatasi and P. sergenti—two established Old World CL vectors—are abundant, did not react with salivary proteins of Lu. longipalpis [16].
Both LJM17 and LJM11 were recognized by dog sera from Teresina, endemic for canine VL [19]. A recent survey of the sand fly population from this area showed that Lu. longipalpis represented 99.7% of the collection [20]. Different from work done previously, here we detected six different salivary proteins as potential specific markers for exposure to Lu. longipalpis by using recombinant proteins for dogs (figure 5). The dog-biting status of other Lutzomyia species needs to be established before the specificity of these proteins as markers of exposure to Lu. longipalpis can be validated. However, this does not detract from their usefulness as potential markers for sand fly exposure for the evaluation of intervention studies in dogs.
Although we did not test potential cross-reactivity of LJM17 and LJM11 with salivary proteins from other common vectors such as mosquitoes, kissing bugs, and black flies, extensive comparative transcriptomic analysis confirm that these two proteins are unique and distinct from those in the saliva of other arthropod vectors [31]–[35].
In conclusion, we have identified two salivary proteins from Lu. longipalpis, LJM17 and LJM11, that were specifically recognized by sera from humans living in an endemic area of VL. Once tested on a wider scale, these proteins could become an important tool for accurate surveillance of this important vector of VL in Latin America.
We thank Dr. Robert Gwadz and Dr. Thomas Wellems for continuous support, Dr. Ribeiro for his discussions on this work and NIAID intramural editor Brenda Rae Marshall for assistance.
The authors have declared that no competing interests exist.
This work was supported by the Intramural Research Program of the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, US National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Following Freeware - September 2015 releases
This month you can go back to the 1920s as an aviatrix in the film industry or forward to the far future as a man trapped on a crippled spaceship. Horror fans can pursue a serial killer through a castle, or uncover supernatural happenings out in the woods. Those into IT can become a hacker breaking into a computer against the clock or a game reviewer who gets too immersed in the latest release. Alternatively, you can get three games in one in a collected trio of little sci-fi tales. All these await in this month's round-up of releases from the freeware scene.
Steve's Selections
The Boogie Man
Detective Keith Baring is obsessed with his job. He has become distant from his wife and has started adopting increasingly harsh methods to bring criminals down. When his latest case attracts unwelcome media attention, the chief of police orders him to take a holiday. Not only that, he provides tickets for Keith and his wife to stay in a new resort at a remote castle. But this vacation proves to be no rest cure for Keith. In the middle of the night, his wife and the other guests are taken by the mysterious Boogie Man. Only by playing this twisted fiend at his own game will Keith see any of them alive again.
Uri's The Boogie Man is a dark tale with new horrors lurking around every corner. This is not a game for the young or faint-hearted, as there is a significant amount of gore and other horrific occurrences. The main graphical view is a top-down retro role-playing game style. The majority of time is spent in the castle, with its rough stone walls and corridors. The decorations vary, from the comparative comfort of the guest rooms with plush seating to a storeroom covered in blood. The action largely takes place in the dark, so only the area immediately around the protagonist is clearly visible whilst indoors. Though small and moderately pixelated, the characters have enough distinct features to be individually identifiable. Occasionally there are still images rendered in a more detailed manga style for important story cutscenes. For much of the game there is no music, with only a soft whispering wind or, where appropriate, running water. The exceptions involve the appearances of the Boogie Man, who likes theatrical music to back him up, and action music for some of the more dramatic sequences. The game is far from silent though, with footsteps echoing on the floor, creaking doors and a multitude of unpleasant sound effects when helping the villain's victims. The game is also fully voiced, with the Boogie Man especially providing a disturbingly effective performance.
Control is done through the keyboard, with arrow keys to move and Z to interact with whatever Keith is facing. X opens a small menu that allows access to the inventory, from which you can select an item to use instead. At the start of the game you will only have a small part of the castle to explore, most of which you will have seen in the relatively gentle introduction. As you progress, you will achieve greater heights and depths as you unlock doors and secret passageways. You will acquire a modest inventory, often in quite unpleasant fashion, and will need to be creative in your use of much of it. There are also secret codes to resolve and strange machines to operate. The latter are often creations of the Boogie Man, set to kill you or others. Whilst you will usually have some advance warning, it is advisable to save your game regularly as sudden death is a frequent possibility. The same is true for your fellow guests, and there are a couple of times when you will be working against the clock to save someone from a gruesome end. The story comes out naturally over the course of the game, with the reason for Keith's obsessive behaviour becoming a key factor. There are five available endings, mostly determined by whether some of the other characters live or die.
The Boogie Man can be downloaded from the developer's website.
Editor's Note: This game was originally credited to the wrong developer. We have since corrected the error. Sorry, Uri!
Jacqueline White: Bad Trouble in the Red Desert
It is the 1920s and the action film industry is booming. For aviatrix Jacqueline White, this is a great opportunity. Not only does she take cameras aloft to capture aerial shots of the action, she even gets to take part herself. Signed up for the latest movie from Two Bad Pennies studio, her work takes her to a popular film location, a desert with an abandoned mine. But unexplained accidents have been putting other crews out of business recently. Fearing they will be targeted next, studio head Michael Edwards has warned Jacqueline to keep an eye out for suspicious behaviour. Soon it becomes apparent that "bad trouble" may not just be part of an upcoming film title.
Grok and HandsFree have very much captured the spirit of the Roaring Twenties in Bad Trouble in the Red Desert. The graphics are largely presented in a watercolour style, though black lines have been used to give important characters and objects definition. You will visit such contrasting locations as the studio's prop-filled storeroom and the barren sands of the titular desert. Jacqueline herself is clad in a sensible period pilot's outfit, complete with scarf. She is smoothly animated, though most of the other characters she meets are static. Backgrounds are full of detail, including many posters for the studio's past successes. The musical accompaniment is very much in keeping with the setting, being a variety of silent film music that changes to suit the current location and action.
Interaction is largely done with a single left mouse click. The cursor is a simple arrow most of the time, but when pointed at a hotspot it changes to a default action (usually look) and right-clicking cycles through the other possible interactions for that hotspot. At the start you will merely be delivering films and props, though locating these is not as easy as it first appears. Later you will investigate the injury of stuntman and uncover a conspiracy that goes beyond the studio. Along the way you will need to crack a written code and gain access to a secret club, a task that requires multiple clicks for each individual action. There is a hint system in the menu bar, which will give you two hints per puzzle, one subtle and one blatant. There are also a couple of arcade sequences, though they come with an option to skip if so desired. You will have extensive conversations with the various characters, ranging from danger-loving stuntmen to an old rich banker who is unhappy that his daughter wants to be an actress. The plot moves along nicely, emulating the adventure films that inspired it.
Jacqueline White: Bad Trouble in the Red Desert can be downloaded from the AGS website.
The Lone Planet
Michael Starmik always felt like there was something missing from his life. When the chance to enlist in a scientific expedition going beyond the solar system came about, he was all too ready to sign up. True, he was only able to achieve a lowly rank, but he was still excited to be travelling out into space. Then one day, whilst relaxing in his quarters, he feels a terrible impact across the ship when it is struck by an asteroid, suffering severe damage across all decks. If Michael is to survive, he must make his way to an escape pod quickly. But first he needs to work out how to open the jammed doors of his quarters.
In The Lone Planet, CNC Acolythe puts the player right in the middle of a major disaster. The graphics are displayed in moderately low-resolution, with both characters and backgrounds being noticeably pixelated. However, good use of colour has been made to distinguish different parts of the scenery, so items are easy to spot. You start off in your one-room quarters, which have survived the impact largely unscathed. Once you have escaped your initial confinement, you find the rest of the ship has not fared so well, with collapsed corridors and some parts open to space. Background noise is mostly a low rumbling as of remote machinery, though some areas have dramatic sci-fi music. There are plentiful sound effects throughout, including the crackling of damaged electronics and the hiss of pneumatic doors.
Control is handled by left-click, with right-click cycling through walk, interact, look and talk options. These same actions can be selected from a menu at the bottom of the screen, where the inventory is also situated. You have two main problems in accessing other areas of the ship. Firstly, many doors are damaged or sealed, requiring you to find alternative ways of getting past them. You are also one of the lowest ranking crew members, thus having only limited access in the first place. Fortunately a robot and another crew member have survived, and both are able to aid you. You also gain a utility kit which contains general tools that are useful in a variety of situations. The overall tone of the adventure is humorous, though given the death and destruction around you this is slightly black in tone. It is also possible to die, though only by taking risky actions, so saving regularly is advisable.
The Lone Planet can be downloaded from the AGS website.Continued on the next page...
The Boogie Man
Platform(s): PC
Jacqueline White: Bad Trouble in the Red Desert
Platform(s): PC
The Lone Planet
Platform(s): PC
Escape the Game
Platform(s): PC
Platform(s): PC
The Darkest Woods
Platform(s): Mac, PC, Linux
Platform(s): PC
Vortex Point: Time of your life Carnival
Platform(s): Mac, PC
Our regular round-up of freeware homebrew adventure games
Oct 29, 2017
Sep 29, 2017
Aug 30, 2017
Jul 31, 2017
Jun 30, 2017

subset: commoncrawl


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Quick Details
- Type: Code Reader
- Voltage: 12V
- Power: 9W
- Certification: CE FCC ROHS
- Applicable Models: universal cars
- Place of Origin: Guangdong, China (Mainland)
- Brand Name: Launch, Launch Creader VII+
- Model Number: Creader vii+
- Warranty: 2 Years
- Item Type: Auto Code Reader scanner
- Language: English,Spanish, French, Russian, German, Italian
- Weight: 0.7KG
- Version: 100% Original and Newest
- Delivery time: 2 working days
- Connection: standard 16 PIN OBD diagnostic interface
- Shipping terms: DHL,EMS,FEDEX,UPS,TNT
- Software Update: One Click Upgrade
- Special Feature: The same function as launch CRP123
Packaging & Delivery
|Packaging Details
|Original packing+carton box
|Delivery Time
|Within 2 working days after payment
Original Auto Code Reader Launch X431 Creader VII+ Creader VII Plus Update Via Offical Website OBDII Scanner Same as CRP123
Launch CReader Professional Creader VII+ is a new generation of core diagnostic product for professional and individual users released by LAUNCH, a DIY diagnostic product whose development is based on DPU platform composed of chip DP431 and JV700 as core components which are independently developed and designed by LAUNCH. The main functions do not only include OBD / EOBD standard protocol diagnosis, but also cover professional diagnostic software for multiple models which can perform comprehensive diagnosis on four main ECU's (ENG\ABS\SRS\AT) of vehicle.
1.3.5'' TFT color display, Higher resolution, sharper colors
2.Works on ALL 1996 and newer vehicles (OBDII& CAN)
3.Retrieves generic (P0, P2,P3 and U0), manufacturer specific (P1, P3 and U1) codes, and pending codes
4.Easily determindes the cause of Check Engine Light
5.Turns off MIL, clears codes and resets monitors
6.Displays DTS definitions on the 320*480 TFT corlor screen
7.Views freeze frame data
8.Displays monitor and I/M rediness status
9.Reads live PCM data stream
10.Displays live O2 sensor test data
11.Performs modules present test
12.Enhanced OBD II Mode 6
13.Graphs Display, Data Record& Replay and DTCs tips guide users to the root cause of trouble code faster, 14.saving diagnosing and repari time
15.Retrieves vehicle information (VIN, CIN and CVN )
16Support large extension memory card
17.Multilingual menus, DTC definitions- English, Spanish and French, etc
18.Extremely easy to use and highly reliable
19Internet upgradeable
20.Prints data via PC
Screen:3.5', 320*480 TFT LCD true color display
Input voltage:9-18V
Connection:standard 16 PIN OBD diagnostic interface
Working temperature:0-50°C
Storage ambient temperature:-20 to 70°C


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Fired Morgan Stanley banker Galen Marsh avoids jail for stealing data on 730,000 of the bank's clients data, later put online.
Tag: banker
Banker Who Was Pimping Out his Wife to Close Business Deals Resigns
Scandalous Jefferies Banker Sage Kelly, Known for Coke and Molly Fuled Sex Romps and for Pimping Out his Wife to Close Business Deals Resigns.
Banker Accused of Recording His Sex Romps Adds Victim on Facebook
Former Morgan Stanley Banker John C. Kelly Accused of Recording His Sex Romps friend requests Victim on Facebook.
British Banker Goes 'American Psycho' and Brutally Kills 2 Butannas During Wild Sex Romp
Brutal banker Rurik Jutting charged after killing 2 prostitutes in a luxury apartment in Hong Kong.
Banker Sued over Molly and Coke Fuled Sex Romps Settles Divorce
Banker Sued over Molly and Coke Fuled Sex Romps Settles Divorce
Another Deutsche Banker Commits Suicide in NYC
Another Deutsche Exec. Calogero Gambino Commits Suicide in NYC
Pimping Wars – Ex IMF Chief DSK Sues Sex Club for Using DSK as name of a Brothel
Ex IMF Chief Dominique Strauss Kahn, more famously known for his frequent sex romp scandals where he's been accused of aggravated pimping charges and beating up some of the talent is back in the media. This time, he's suing a brothel for using his initials DSK in the venues marketing. The self branded bad ass party boy
Female Banker Arrested for Hitting a Guy with a Bottle in NYC Nightclub
Female banker Julia Kadioglu was arrested for allegedly hitting the guy with a bottle in The Electric Room at the Dream Downtown in Manhattan.
JP Morgan Agrees to Pay $1.5 Million to Settle Sex Discrimination Lawsuit
JP Morgan Agrees to Pay $1.5 Million to Settle Sex Discrimination Lawsuit Against the Bank
DAVOS: Banker Says Letting Banks Fail is Essential to Rebuilding Public Trust
Banker Says Letting Banks Fail is Essential to Rebuilding Public Trust.
Banker Films Having Sex on Camera – Blames Sex Tapes on Dog
Banker John C. Kelly Films Sexual Encounters and Blames Sex Tapes on Dog.
Sex Drugs and Bad Banking – Minister Turned Banker in Sex Drugs Scandals
Sex Drugs and Bad Banking Paul Flowers, the former chairman of Britain's Co-operative Bank. Two Years ago Paul Flowers, chairman of the Co-operative Bank, was having a jolly time of it. After he'd enjoyed the hospitality of the directors' box…
Banker Labeled "Crazy Miss Cokehead" Wins Sexual Harassment Set to Win Millions From Bank
Banker Labeled "Crazy Miss Cokehead" Wins Sexual Harassment Claim – Set to Win Millions From Bank. A 32-year-old female banker London bank says she was smeared as "chemically dependent minigarch daughter" and "Crazy Miss Cokehead" when she blew the whistle…
Banker Dies After Sex Romp With Mistress
45- Year Old Banker Dies After Sex Romp With His Undergraduate Mistress
Banker Arrested for Raping a Woman During Naked Pool Party in Hamptons
Goldman Sachs banker Jason Lee arrested for 'raping 20-year-old woman during naked pool party' at $33K-a-month house he rented with his wife in the Hamptons Jason Lee, 37, denied the charges and posted $20,000 bail in cash. The banker, Jason…
Police Looking For Hitman of High Profile Banker
Police looking for a hitman of a founder of Arab-Malaysian Development Bank Hussain Ahmad Najadi, after he was shot dead in a parking lot in Kuala Lumpur.
Big Banker and Big Pimping – DSK Still Facing Aggravated Pimping Charges
Big Banker and Big Pimping – DSK Still Facing Aggravated Pimping Charges
Banker Playboy Robert Wheeler Introduced Strippers to His Kid – Lawyer Claims
Party Loving Banker Playboy Robert Wheeler Introduced Strippers to His Kid – His Former Divore Lawyer Claims.
Swiss Banker Goes Jail for Sex With Underage Prostitute
Juerg Buergin, former Swiss Banker sentenced 3 months in jail for sex with underage Singaporean prostitute.
City Banker Fired Over Sex-Bias Case Against Another Bank
When it comes to City bank – they have previous experience from sexy banker chicks suing them for sex related lawsuits- like Debrahlee Lorenzana, who sued Citibank for being too sexy.
banks-funnyNow City banker Latifa Bouabdillah sacked over sex-bias case against former bank.
Latifa Bouabdillah claims Commerzbank dismissed her when it found out she was suing Deutsche Bank for discrimination
Another Ex Banker Stripped for Playboy – Hedgeho Wild on Wall Street
Another Sexy Ex Banker Quit Her Job And Stripped For Playboy – Hedgeho Playboy can convince the sexiest women in all industries to strip. Since 2009 Playboy started convincing women on Wall Street to strip – take all their clothes…
Bankers To Bangers – Porn Star Banker
Porn for bankers Banking and banging In a unconventional but fun way, international retail specialist Hans Eysink Smeets describes how porn and other sectors had to drastically reinvent their retail models, managed to survive, and created a whole new set…
Angry Banker Quit Because Didn't Get Laid In The Office
No Models No Sex and No Strippers In The Office For This Finance Guy Some offices have all the fun stuff and strippers which can make an average guy very mad. That for sure is the case for this angry…
Find out The Outcome Of Too Sexy Fired Citi Banker and Hot Photos
Too Hot Fired Banker Said No To Playboy But Check Out These Sexy Photos More Women Who Were Fired For Being Too Hot
Hot Fired Citi Banker Warnes NJ Woman About Her Lawyer
Banker warns NJ Woman who fired for to big boobs that her lawyer is using her for media exposure Gloria Allred's former clients, too-hot-for-work banker Debrahlee Lorenzana, said of the high-profile celebrity litigator left as soon as the cameras. Lorenzana,…
Egyptian Banker Who Sexually Assaulted a Maid at Pierre Hotel – Judge Recommended Today to Pay $350K
Manhattan federal judge recommended today that an Egyptian banker who sexually assaulted a maid inside his $900-a-night room at The Pierre pay the woman $350,000 An Egyptian banker who used Strauss-Kahn's sexual hijinks with a hotel maid should cough up…
Sex Addicted Banker Was Banging a Co-worker at Work for 5 years
Sex Banker who had Sex at work numerous times wants his job back A former Bank of Montreal manager who was fired for having sex several times with a female employee during work during 5 year period hours wants his…
City Banker Convicted Serial Rapist
"It's your lucky day", Citi Financier's words to his sex attack victim. Brian Witty, 41, a former captain in the Parachute Regiment, is convicted in three rapes and a sexual assault over a 16-year period. Despite being arrested after each…
Murder, Sex and Insanity-Ex-GE Manager Claims Insanity in Atlanta Killing of Former JPMorgan Banker
Hemy Neuman, a GE Energy manager, put on a fake beard, drove a rented minivan to a suburban Atlanta day care center and shot an ex-JPMorgan Chase & Co. (JPM) banker dropping off his son, defense lawyers said. Neuman's lawyers…
Goldmans Banker Screwing Hookers and Takes Regular Trips Strip Clubs and Brothel
A City banker who boasts of sleeping with prostitutes worked on the deal at the centre of the Goldman Sachs 'fraud' scandal. Eton and Oxford-educated Tetsuya Ishikawa regularly travelled abroad and admitted: 'The first thing I asked on arriving was,…
A laid off Deutsche banker died after a kinky fantasy role play with two hookers. Last year, Colin Birch, a recently laid off assistant vice president at Deutsche, hired a bunch or hookers to fulfill a kinky torture fantasy. The…


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English - French - Dutch - Italian - German - Russian
Furthermore we take care to keep the groups small and the atmosphere easy-going.
Our classrooms are equipped with air-con for the hot summer months, TV, video and all the teaching materials are available in the different languages.
The diving equipment we use is new and we take care to maintain it in a proper state. We offer special rates for instructors who bring their own students and they can also make use of the diving center facilities to give their courses. Below, we have made an overview of the different beginning, advanced and specialist courses with some specific information per course.
If you're 8 or 9 years old and want to take a look underwater without having to hold your breath all the time, there is the Bubblemaker program for you. You will get a set of equipment and go under water, accompanied by an instructor, from the beach or swimming pool. Afterwards we'll give you a wall certificate, a bag, a tattoo, a sticker...
Prerequisites: - min. 8 years
- Maximum depth: - 2 meters
Prerequisites: - min. 10 years
- Maximum depth: - 12 meters
PADI (Junior) SCUBA DIVER This pre-entry level course is perfect for people having only one week holiday and who plan to do other things outside diving. It is a quick and easy program which is perfect if you only want to dive a couple of times a year. With the Scuba Diver certification you can dive afterwards everywhere in the world under the supervision of a PADI Divemaster, Assistant Instructor or Instructor. In this way you will explore the underwater world with the comfort and safety of having an experienced guide with you all the time. During 2 days, you will get some theory including videos, you will learn some skills in the swimming pool or from the beach and you will make 2 dives in the open water.
Prerequisites: - being able to swim - min. (10) 15 years Maximum depth: 12 meters
Duration: - 2 - 3 days
PADI (Junior) OPEN WATER COURSE Upon completion of this entry-level course you will receive a PADI Open Water Diver license which is recognized in every diving center and allows you to go diving all over the world. The course normally takes 4 days, in which you will go through 5 academic lessons and 5 confined water sessions during the first 2 days. The 4 open water training dives will be made on the final 2 days. Knowledge of basic diving theory is covered by video, manual and instructor presentations. Diving skills are developed in the swimming pool or in the confined water from the beach. During the open water dives you will get the opportunity to apply the knowledge and skills you learned before, always together with your instructor.
Prerequisites: - being able to swim - min. (10) 15 years Maximum depth: - (12-18) 18 meters Duration: - 4 - 5 days
Referral Courses The referral Course is meant for those who are certified as Scuba Divers and want to upgrade to Open Water Diver, or for those who have completed their theory and confined water in one place, but want to do their open water dives in the Red Sea, or for those who want to finish their course. What exactly you will need to do, to complete the Open Water course, depends on the type of referral you have. You can contact us for more details and information.
Private Open Water Course During 4 days you will have a private instructor who, in this way, will give you all the attention and time you need to complete the course.
Duration: - 1 day
PADI (Junior) ADVANCED OPEN WATER COURSE
This course focuses on expanding your diving skills during an organized and supervised experience which takes 2 days minimum. You will complete 5 dives of which there is a mandatory deep and a navigation dive to do + 3 more dives of your choice. The most popular dives are: drift, wreck, night, photo, scooter and naturalist. There is some reading to do and you will get some extended pre-dive briefings but there are no classroom sessions. The Advanced Course can also be done during live aboards. Prerequisites: - min. (12) 15 years - having an entry-level diving certification from any organization Maximum depth: - (21) 30 meters Duration: - 2 - 3 days
PADI SPECIALTY DIVER COURSES These courses are designed to further your knowledge and skills in specific diving activities which interest you. You will get hands on practice and some theory sessions. All specialties can be done either as a complete course or dive by dive. The dives you did during your PADI Advanced course can be counted as the first dive of the related specialty. We offer following Specialty courses: 2 DIVES: Photo / Boat / Drift / Dry Suit / Multilevel / Naturalist / Peak Performance Buoyancy / Enriched Air (Nitrox) / Scooter / AWARE Fish Identification / Full Face Mask 3 DIVES: Night / Underwater Navigator / Video / Ray Rebreather 4 DIVES: Deep / Wreck / Search and Recovery 1-2 days: Equipment Specialist: in the diving center
PADI E.F.R. (Emergency First Response) During this course you will get basic training in CPR and First Aid procedures, which you will after be able to use in any incident. There is a video to watch, we'll do some practical sessions and apply the learnt techniques in scenarios. The course is based on current medical guidelines within the US and UK and is subject to changes. The course meets the requirements to enroll in the PADI Rescue Diver, Divemaster, Assistant Instructor and Instructor as well as in the Dan Oxygen Provider course. Prerequisites: - none Duration: - one day or 2 half days
PADI (Junior) RESCUE DIVER COURSE The Rescue Diver course is designed to develop and expand your knowledge of your own and other divers safety. The Dan Oxygen Provider Course is recommended in conjunction with the course to give you a better understanding on how to correctly apply oxygen in an emergency.
Prerequisites: - min. (12) 15 years Duration: - 3 - 4 days
PADI (Junior) MASTER SCUBA DIVER Master Scuba Diver is the highest rating you can get as a recreational, non-professional diver. To reach this level you have to be an Advanced Diver and Rescue Diver with 5 PADI specialties. Prerequisites: - min. (12) 15 years
Scuba Review consists of repeating the basic diving theory and a supervised confined water session with a PADI Instructor who will help you tune up important diving skills such as buoyancy control, breathing from an alternate air source, equipment assembly... Afterwards you can decide to take an optional supervised open water dive to get reacquainted with the underwater world. Prerequisites: - be a certified diver Duration: - App. 2 hours
PADI DIVEMASTER COURSE The Divemaster rating is the first step on the professional career path. The course is designed to elaborate your diving knowledge and skills to a professional level. It will enable you to guide certified divers and assist with any training activities conducted by a PADI instructor. The course includes academic knowledge development on 12 topics. You will learn this theory partly by self study and partly through instructor presentations. There is an exam on 8 topics. We advise you to get the Divemaster manual, Encyclopedia and Knowledge Workbook before you start the course. This allows you to begin studying and to prepare at your own pace. There are also some watermanship requirements to fulfill, like a 400m swim, 800m snorkel swim, 15 min. tread and a 100m tired diver tow. We will asses your rescue skills and look how you manage a stressful situation. You will learn how to make underwater maps and develop your knowledge on being a dive guide by going through all the aspects of the job. Another thing we will teach you is how to assist with student divers in training. This will enable you to actively take part during the courses, conducted by your instructor. As a Divemaster you can enroll in the PADI E.F.R. Instructor course. Prerequisites: - proof of 20 logged dives (to obtain certification, a total of 60 logged dives is needed) - min. 18 years - medical statement not older than 12 months, signed by a physician Duration: - min. 10 - 12 days (also possible as a internship)
PADI ASSISTANT INSTRUCTOR COURSE This course is the following step on the professional career path. We will show you how to teach and we will keep your diving knowledge and skills at professional level. After the course you will be able to assist a PADI instructor with all training activities. Further more you will be allowed to independently teach and certify non-diving specialties and conduct Bubblemaker and Discover Scuba programs and the Peak Performance Buoyancy Specialty course. Under the indirect supervision of an instructor, you may give academic elaborations for any PADI course. During the course you will assist in 3 PADI certification courses in a way similar to the Divemaster course. Further more you will go through the same subjects and assignments as during the Instructor Development Course. Many people choose to take this course as a preparation for the IDC. To obtain the certification, you will need to successfully complete the 5 subjects theory exam and a Standards and Procedures exam at the end of the course. Prerequisites: - be a PADI Divemaster - min. 18 years - proof of 60 logged dives - medical statement not older than 12 months, signed by a physician Duration: - 5 days minimum


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Torikoe Shrine was established over 1,360 years ago in Torigoe in the Taito ward, where you can still find a good old Tokyo shitamachi (physically lower area) atmosphere. Imperial Prince Yamato Takeru no Mikoto is enshrined as its main god. On the 8th of January every year, Tondo Yaki, where people burn used paper amulets to send off the god they welcomed on the new year day and wish for their well-being for the year, is held. At the Torikoe Festival in June, people parade carrying the Sengan Mikoshi portable shrine, which is believed to be the heaviest mikoshi in Tokyo. Many people come to see this festival because people with a tengu (a supernatural mountain spirit with red skin and a long nose) mask, Tekomairen dance groups, and children waving five-colored flags joins the parade. Many other traditional events such as Chinowa-kuguri (a rite to dispel the source of disease and defilement) and the Suijosai water festival are also organized. Everyone can visit the shrine, and it is believed that the enshrined gods protect you from and fend off evils. An 8-minute walk from the west exit of Asakusabashi Station on the JR Line or a 5-minute walk from Kuramae Station on the Toei Asakusa Line.
Location Information
- Address
2-4-1, Torigoe, Taitou-ku, Tokyo, 111-0054
- Nearest Station
Asakusabashi Station
・ JR Sobu Main Line
・ Toei Asakusa Line
West Exit
8 minutes on foot
- Phone Number
- 03-3851-5033
Our staff may only be able to communicate in Japanese.
- Hours
- 9:00am - 5:00pm


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MOVIE SYNOPSIS [ General Audiences ] With the actor direction of Nelson Pena, Carlos Vasquez heads the cast of this feature film originally of USA, Dominican Republic and Puerto Rico, with original script in English (with subtitles), whose premiere was scheduled for 2013. You can watch A South Bronx Family online on TV cable or theaters with original audio in English at different dates. This film premiered in theaters in 2013.
Rating: 5 by 10 users./
Runtime: 90 minutes
Carlos Vasquez, Wendy Perez, Keath Perez, Maria Ortiz, Xiomara Perez, Tania Morales, Brittany Morales, Joesph 'Chuckie' Suarez, Elba Perez and Louis Lugo.
Documental, Drama.
90-minute film (full movie) directed by Nelson Pena.
The original audio of A South Bronx Family has dialogues in Dolby Digital, it was produced in USA, Dominican Republic, Puerto Rico.
It premiered in cinemas or television in 2013.
Nelson Pena.
Nelson Pena, John P. Rigores, Craig Blair, Alci Enrique Medina, Damon Middleton and Armando Guareno.
This film was produced and / or financed by A South Bronx Family.
Composed by Robert Julian.
Without commercial breaks, the full movie A South Bronx Family has a duration of 90 minutes; the official trailer can be watched in streaming on the Internet. You can watch this full movie free in EnglishDolby Digital in movie TV channels, renting the DVD or with VoD services (Video On Demand player, Hulu) and PPV (Pay Per View, Netflix).
See the page of this full movie, A South Bronx Family in FULLTV Movies.


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Volunteering With ESAMA
ESAMA is seeking volunteers to assist us in carrying out our mission to provide information, education, and awareness about cultic/high demand groups. Time commitments may vary depending on the volunteer position.
We are currently looking for volunteers for the following capacities:
- Board Members with prior experience in non Profit boards, legal work or accounting background.
- Grant writing/fund rising-with some prior experience in one of these areas.
- Public speaking which involves assisting with community presentations.
- Special events which could involve assisting with the event organization, publicity, setting up, registrations etc.
- Office work which could involve organizing material from the internet or data entry of names etc. Knowledge of basic computer skills is needed.
Please contact our office at (780) 452-1830 if you would like more information on the above, or fill out the inquiry form below.


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The Iowa Republican Party scheduled a second debate for GOP presidential candidates the week before Iowa's caucuses next February.
The Iowa GOP said it will co-host a debate with Fox News set for early February before the caucuses. The party has already scheduled a debate in August as a precursor to the Ames Straw Poll, an early indicator of voters' preferences among candidates before the Feb. 6 Iowa caucuses.
"Iowa is once again taking center stage as Republican voters embrace their responsibility of starting the nomination process, and I am proud to bring an historic piece of that process to Siouxland," state GOP Chairman Matt Strawn said in a statement. "No longer will Republicans in Northwest Iowa have to settle for watching high-profile caucus debates hosted hundreds of miles away, they will be active participants in the process."
While many potential Republican presidential candidates have been reluctant to formally declare their candidacies so far, by August the 2012 field should largely be settled on the GOP side by the first debate. Moreover, participation in the debate could send signals about which candidates intend to participate in the Iowa caucuses.
The second debate, scheduled right before the caucuses, could be the last major opportunities for GOP candidates to showcase themselves before caucusgoers.
Updated 1:00 p.m.


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Makkan Peda/Jamun (Pack Of 3)
Order Before : 12pm
Get Deliver After : 4.30pm (Same Day)
Serviceable Areas :
Abhiramapuram, Alwarpet, Anna Salai, Broadway, Egmoe, Foreshore Estate, Gopalapuram, MRC Nagar, Mandhaiveli, Mannadi, Mount Road, Mylapore, Paris, Broadway, RA Puram, Royapet, Santhome, Sowcarpet, Triplicane.
West Mambalam, T Nagar, Teynampet, Nandanam, Saidapet, Kotturpuram, Adyar, Besant Nagar, Thiruvanmiyur.
Anna Nagar West, Anna Nagar East, Mogappair, Koyambedu, Chinmaya Nagar, Virugambakkam, Valasaravakkam, Saligramam, Alwarthirunagar, Vadapalani, Kodambakkam, Valluvar Kottam, Shenoy Nagar, Choolaimedu, Nungambakkam.
Ashok Nagar, K K Nagar, Guindy, Alandur, Adambakkam, Puzhuthivakkam, Madipakkam, Nanganallur, Velachery, Eekkattuthangal.
Ambattur, Korattur, Padi, Kolathur, ICF, Villivakkam, Ayanavaram, Kellys, Purasawakkam, Perambur, Thiru Vi.Ka.Nagar, Agaram, Kilpauk, Chetpet.


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Here's a question: Does the U.S. have a retail crisis — or a small business crisis?
Here's an answer. We have both.
Which is why "fixing" retail must include giving SMB merchants the tools they need to compete in the very dynamic environment that now defines Retail U.S.A.
Let me explain.
Chevrolet may use "the heartbeat of America" as its advertising tagline, but it's small business that everyone believes keeps the heart of the U.S. economy pumping. Twenty-six million strong, employing 50 percent of the workforce and creating 65 percent of all new jobs, SMBs and storefronts that line the side streets and main streets of our cities and towns are the fuel that keeps our economic engines humming, roughly 100 million Americans employed and what feeds a thriving middle class.
Well, that used to be the story line.
The truth is that America's small business engine is sputtering and has been in a gradual decline for the better part of the last four decades.
That somewhat revised storyline has also been somewhat of a well-kept secret.
Between the late 1970s and the mid-2000s, like the sun rising in the East, the U.S. could count on between 500,000 and 600,000 new businesses opening every year. Things went off the rails, not surprisingly, in 2008 — courtesy of the financial crisis.
But unlike the comeback stories of past recessions, small businesses haven't come back this time. After a high of 614,024 SMB openings in 2014, the Census reported that only 452,835 firms were started in the U.S. that year.
Only, you say, but that's not too shabby — and at least it shows forward progress, right?
Economists don't think so.
They're troubled by these numbers because they believe we've had adequate time as an economy to get the SMB growth engine revving again. And they fear that the impact on the vitality of the local economies that this void has created won't be fully felt for a decade or more.
Of course, business openings are just one part of this story: Business exits (AKA failures) are the flip side of that small business coin — and where those same dismal scientists say there's even more cause for concern.
Small businesses are also risky businesses, and the Small Business Administration tells us that 60 percent of them won't make it past their first birthday, never mind reaching their second, third or magic five-year milestones. Only a third, they say, will ever cross into double-digit territory and celebrate a decade of being in business.
Read More
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MOVIE SYNOPSIS [ General Audiences ] With direction of Ed Burke, Beyoncé Knowles and Ilan Y. Benatar, this film in English (original language) was presented in 2013. This film, a creation of Beyoncé Knowles, Ilan Y. Benatar and Ed Burke, was produced in USA. You can watch Beyoncé: Life Is But a Dream online on TV cable or theaters with original audio in English at different dates. This film premiered in theaters in 2013.
Rating: 5 by 1449 users./
Runtime: 88 minutes
Beyoncé Knowles, Ilan Y. Benatar, Ebony Knowles, Solange Knowles, Mathew Knowles, Kelly Rowland, Michelle Williams, Juelz Knowles, Jay Z, Champion, Mario, Tina Knowles, Laurent Bourgeois, Larry Bourgeois, Kimmie Gipson and Amandy Fernandez.
88-minute film (full movie) directed by Ed Burke, Beyoncé Knowles and Ilan Y. Benatar.
The original audio of Beyoncé: Life Is But a Dream has dialogues in Dolby Digital, it was produced in USA.
It premiered in cinemas or television in 2013.
Screenplay written by Beyoncé Knowles, Ilan Y. Benatar and Ed Burke.
Ed Burke.
Beyoncé Knowles, Lee Anne Callahan-Longo and Bill Kirstein.
This film was produced and / or financed by Parkwood Entertainment and ICM Partners.
Composed by Ben Salisbury.
Yleisradio (YLE), Home Box Office (HBO) and BBC Worldwide.
Without commercial breaks, the full movie Beyoncé: Life Is But a Dream has a duration of 88 minutes; the official trailer can be watched in streaming on the Internet. You can watch this full movie free in EnglishDolby Digital in movie TV channels, renting the DVD or with VoD services (Video On Demand player, Hulu) and PPV (Pay Per View, Netflix).
See the page of this full movie, Beyoncé: Life Is But a Dream in FULLTV Movies.


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|the bathroom layout
below are a few photos that inspired the design of the first floor bathroom. this bathroom is going to have an enclosed shower / steam shower, a custom designed vanity (design is in the process), and tons of natural light from three windows so i wanted it to feel light and airy and to reflect the contemporary farmhouse feel that we are going for with this house.
|aqua glass tile and a herringbone marble mosaic floor
(mueller nicholls cabinets and construction via houzz)
|love the large format glass tiles in a grey-green color
(studio m design via houzz)
|white horizontal wood panelling for the walls
i put together this mood board which summarizes my wishlist for this bathroom. björn and i had already decided to run the hardwood cherry floors into this lower bath. we love the look of the rich cherry with beautiful painted cabinetry and with the radiant underfloor heating it will be toasty warm underfoot even on the coldest days!
1. herringbone carrera marble from AKDO
2. glass tile in "whisper" from Dal Tile
3. sherwin williams 6218 tradewinds- ceiling
4. sherwin williams 6168 modern white- trim and walls
5. vanity lighting - from durango light art (manu. unknown)
6. infinity mirror- room & board
7. archer self-rimming sink & archer lavatory- kohler
8. shaker style custom cabinetry
9. cherry hardwood floor
10. persuade two-piece elongated toilet- kohler
11. artwork
i really would love to put horizontal wood panelling painted white on all of the walls, i think it looks clean and contemporary but definitely give some farmhouse charm to a space. it will also make the walls have a ton of character so that i can hang minimal artwork. speaking of artwork i would love to incorporate profile silhouettes of björn and i as children somewhere in the house and i think the bathroom would be a great place!
my dreams for the steam shower include soft grey-green glass subway tile walls and ceiling, and a beautiful herringbone marble mosaic floor. i love the luxury of the marble paired with the simplicity of the glass tiles. because we have to tile the entire height of the walls and the ceiling due to the steam shower aspect i may need to add an accent stripe of white to break up the grey-green tiles. all of our fixtures for the shower are from kohler in a chrome finish.
the vanity is being designed by moi and will be built by björn and matt. i am leaning toward a simple shaker style painted the wall color, with a grey concrete or engineered stone top. i will share the design with you as soon as it is more complete. we are using kohler's archer series sink and faucet which i love because it has a very clean look with some traditional styling. and the toilet has actually been decided upon for months. björn fell in love when he saw this toilet and its sleek "spaceship" like design. (his words) i love it because it will be so easy to clean and has a fun dual flush button on the top.
i would love to paint the ceiling of this space a grey-green color like sherwin williams tradewinds, to compliment the glass subway tile. i hope that this first floor bath will


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Anderson Hart Jr. tends to the flower beds outside the Capitol building in Atlanta. The General Assembly will begin its new session today, while Gov.-elect Sonny Perdue will start a new tradition of "inspecting the troops" in which he reviews an honor contingent of state troopers at the Capitol grounds. AP Photo
ATLANTA -- When Sonny Perdue takes the oath of office today as Georgia's first Republican governor since 1872, he will find new allies among governors in adjoining Deep South states.
On the same rainy November night that Perdue ousted Democrat Roy Barnes from office, voters in Alabama and South Carolina also threw out incumbent Democratic governors in favor of GOP candidates.
The overwhelming success of the Southern Republicans served as further testimony to the GOP's nationwide sweep of the mid-term elections.
In few places was the change as dramatic as Georgia, the only state not to elect a Republican governor during the entire 20th century. Georgia voters also sent home the Democratic leader of the state House and the Senate majority leader, as well as the junior Democratic U.S. Senator, Max Cleland.
However, some say it's much too early for the legislatures of these Southern states to adopt the Republican elephant as their permanent mascot.
''In 2002, it was a good year for Republicans,'' said Charles Bullock, a political scientist at the University of Georgia. ''However, the GOP is not in as nearly a secure position in these states as the Democrats were historically. The Democrats have a strong base of support, and could take back any one of these governorships in four years.''
Still, Democrats suffered great wounds in the 2002 campaigns.
''Democrats blew it,'' said Alabama political scientist Carl Grafton, a professor at the University of Auburn-Montgomery. ''There was historically every reason that Democrats would have picked up seats and they blew it.''
Problems in Georgia
Among the problems plaguing the Democratic campaigns of 2002 were the lack of a unified party message and the appearance that Democrats didn't stand for any particular issue.
Robby River, clerk of the Georgia House, tests voting buttons on each desk Friday in preparation for today.AP Photo
On election day, Barnes was still thought by expert pollsters to have a slight lead over Perdue. But when the returns came in, Perdue bested the incumbent by more than 100,000 votes.
Observers say Barnes was tied to too many negative issues, such as redistricting, Atlanta sprawl and changing the state flag, reducing the size of the Confederate battle emblem on the banner.
In 1998, Barnes won 118 of the 159 counties in Georgia. In 2002, the numbers flip-flopped, with Barnes capturing a mere 41 counties. His regional support disappeared in southern and rural Georgia, where anti-Atlanta and anti-flag-change sentiments ran high.
The battle for partisan control of Georgia's legislature didn't end on election night. Republicans quickly went after centrist Democrats in an effort to steal their support from the Democratic Party.
Perdue's surprise victory led four Democratic senators to jump ship and join the GOP, giving the Republicans a 30-26 majority in the state Senate and the ability to strip Democratic Lt. Gov. Mark Taylor of much of his power as Senate president.
In the House, two Democrats are battling to be elected as speaker, the ruling office of that chamber. One candidate, Rep. Terry Coleman of Eastman, is considered a loyal Democrat. The other, Rep. Larry Walker, holds a more centrist ideology and would be considered a Perdue ally if elected to lead his colleagues.
On the fence
In Alabama, the future of the Republicans as agenda-setters is uncertain.
Voters have flip-flopped five times since 1986 over which political party they want in the governor's mansion.
In 1994, a mere 262 votes separated the candidates for state chief justice, leading to a year-long trial before the Republican candidate claimed the office.
Last year, incumbent Gov. Don Siegelman, a Democrat, narrowly lost his re-election bid by a razor-thin margin of 3,117 ballots among a total of nearly 1.36 million votes. He is now succeeded by Bob Riley, a former state legislator who drew heavy support from big business and agriculture groups.
However, both chambers of the state legislature rest firmly in Democratic control.
Siegelman's close loss was typical of a state where neither political party has a firm toehold over the electorate, Grafton said.
''Over the last 25 years, we've seen certainly a long shift to the Republicans in Alabama,'' he said. ''That shift is about over. Roughly speaking, in Alabama ... about 40 percent of the electorate is hard-core Democrat, about 40 percent is hard-core Republican ... and 20 percent will split their tickets depending on the issues.''
Grafton says that despite Riley's marginal win, how he handles the state's impending budget crisis looms large over his administration and his ability to ask for another term in 2006.
''Democrats will win in four years because in Alabama our financial condition is so awful that Bob Riley's reputation will be in shambles,'' he said. ''I don't think any person from either party could handle it.''
GOP stronghold
Among the trio of Southern states seeing a Republican governor rise to power, none has become more progressively Republican in recent years than South Carolina.
The only of the three states where the GOP now controls the House, the Senate and the governor's mansion, South Carolina now offers the Republican Party an unfettered chance to address state issues.
Such a lop-sided collection of power could prove troublesome for Republicans, said Susan MacManus, a political science professor at the University of South Florida in Tampa.
''The difficulty is the wider the margin of control by one party, the more independent individual members become, so it becomes a intra-party fight,'' she said. ''The governor ... would be on Easy Street, but it's just not going to happen.''
Still, in a state where Republicans won nearly every major election in 2002, there was often little ideological difference between the two candidates for governor, said Blaise Graham, a University of South Carolina political scientist.
''There didn't seem to be any major issue that separated the candidates,'' he said.
For Democrats to make inroads at winning the governor's race in 2006, they will need a candidate who can reach out to minority voters, as well as the middle class, with a strong message, such as Hodge's proposal to create a South Carolina lottery.
''It's going to be difficult for the Democrats,'' Graham said. ''This is such a heavy (President) Bush state.''
Published in the Athens Banner-Herald on Monday, January 13, 2003.
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text:
Revotech Reaches Agreement to Represent BEJED Fiber Optic Communication Equipment in Southern Califo
Revotech Reaches Agreement to Represent BEJED Fiber Optic Communication Equipment in Southern California
Revotech and BEJED reached an agreement that will allow Revotech to begin representing BEJED's line of fiber optic distribution equipment in Southern California. BEJED is the leader in fiber optic technology and is proud to have been manufacturing high quality communication equipment in the USA for over 30 years. "The addition of BEJED's line provides a wonderful compliment to Revotech's current RJAX coaxial line, said Revotech's Steve Rochman, and allows Revotech to provide electrical contractors, installers and structured wiring manufacturers with creative, unique solutions for their customers and clients."
About Revotech
Founded in 2001, Revotech is a Los Angeles, California based company, and a leader in sales and distribution of technology, communication and distribution products. Revotech products are used around the world for a wide variety of consumer and commercial customers including UBS Financial Services Group, Tatung Company, Matsushita Taiwan, Koyo Bearing and Honda Indonesia.
About BEJED
BEJED has manufactured high quality communications equipment since 1972. BEJED equipment is deployed in extensive mission -critical applications worldwide, and has provided OEM solutions to some of the leading equipment providers in the fiber and communications industry. BEJED is proud to be one of just a few major equipment providers in the industry, who still manufacture and assemble in the USA


text:
Rashard Mendenhall
October 9, 2011 by staff
Rashard Mendenhall, Steelers RB Rashard Mendenhall (hamstring) is questionable, but was limited on Friday. If you're not practicing, it is safer to make adjustments for the week. Mendenhall, who has been underperforming, faces a defense allowing 81.3 yards rushing in the position. Look elsewhere if you, such as Isaac Redman gets to see and start Mendenhall, if you can not go. Redman is an injury bye week filler or a No. 3 back in the big leagues. With Mewelde Moore (ankle) he would see most of the carries.
Look for Texans RB Chris Ogbonnaya to serve as the backup Arian Foster. Ben Tate (groin) and Derrick Ward (ankle / shoulder) both will be ready, but only one is active, to seek a decision on game day. Foster is likely to carry the load Ogbonnaya as the No. 2, so that Tate and Ward have no value this week. You can grab Ogbonnaya and hope he sees a dozen touches against a minor run defenses in the league.
Texans WR Andre Johnson (hamstring) had hamstring surgery and plans to return in a couple of weeks. Look for Jacoby Jones (knee), who practiced this week to take over. However, he has disappointed all season and is nothing more than a flex player. Kevin Walter is a worse option, as their coach this year was just a fluke. It's just a bye week or injury fill.
Cardinals RB Beanie Wells (hamstring) is questionable and was limited in practice all week. Find a game-time decision and make backup plans in case. If he plays, inserts it into the lineup after 27-138-3 performance last week.
Steelers QB Ben Roethlisberger (foot) practiced this week and the game will be. However, with only three touchdown passes in four games against a tough Titans' secondary, this is not a week to play with a bad foot.
Chargers WR Vincent Jackson (hamstring) practiced Friday and is probable. Look for him to play. It's a No. 1 receiver in fantasy.
Patriots TE Aaron Hernandez (knee) practiced on a limited basis Thursday and Friday, but remember, these are the Patriots and injuries that could go either way. I hope I can play and should be in all lineups.
49ers RB Frank Gore (ankle) was limited in practice but is probable and should play. However, get back Kendall Hunter to see his share of touches. Gore is a No. 2 back and Hunter is goodbye, week or injury fill.
Falcons WR Roddy White (hamstring) is probable and has a nagging injury more than anything else. It is a must-start with a great matchup.
Packers RB Ryan Grant (kidney) is good to go and there was never any doubt. It is a farewell, weeks or injury fill-in player this week.
Giants WR Mario Manningham is still the starter despite the great work of Victor Cruz the last two weeks. I guess it could be any week or Tails Manningham. Mannigham is a No. 3 receiver for fantasy with a flex or bye week Cruz filler.
49ers WR Michael Crabtree was limited in practice, but offensive coordinator Greg Romano, said Crabtree looks like a 100 per cent. It's time to put it in their lineups with great mourning and bye week set in.
Raiders WR Denarius Moore will continue to see playing time even though the rest of the receivers back to health. However, because there are so many people and Moore is a player or boom and bust is a flexible position player.
Jets WR Plaxico Burress (shoulder) is good to go and is a No. 3 receiver for fantasy this week.
Saints WR Devery Henderson (calf) was limited and, with a full group of receptors, which are more appropriate to leave it on the bench as he is questionable.
Giants RB Brandon Jacobs (knee) is doubtful for a slight MCL sprain. Find a game day decision, but do not expect to leave. It has been underperforming anyway. Look for Ahmad Bradshaw to see most of the carries. Bradshaw is a fantasy No. 1 again as the primary ballcarrier.
Chargers TE Antonio Gates (foot) is out and there is no timetable for his return. Randy McMichael will start, but despite the tight end role in San Diego's offense, not worth picking up. He's just a watch-list player, it is not a great fantasy tight end.
The "B" team …
Raiders QB Jason Campbell (foot) is more of a nagging injury that nothing serious and will be good to go. He remains a quarterback fantasy No. 2.
Buccaneers RB Earnest Graham (hamstring) is good to go this week and will continue its role of functions change of pace and third down. Their only value is in the big leagues.
Seahawks WR Mike Williams (concussion) is out this week. Ben Obomanu will get the start, but this is a great opportunity for rookie Doug Baldwin to see an increase in goals, making him a bye week or injury fill.
Panthers TE Jeremy Shockey (concussion) is good to go and is a No. 2 tight end, but you can play with him as a starter in the big leagues, because of byes and injuries.
Cardinals TE Todd Heap (hamstring) is questionable and a decision will be game time. It has been practiced this week and if not 100 percent that is not worth starting.
Colts QB Curtis Painter brought hope to the owners of the positions of the Colts another skill. He will start his second game and watch to see if you can show any improvement after two consecutive weeks, taking first team reps, probably for the first time in it. Kerry Collins (concussion) returned to practice, but he is No. 2 and the backup if anything is likely painter shows this week.
Chiefs RB Jackie Battle to see more details this week, so keep an eye on it, but does not guarantee a fit of fantasy or a truck yet.
Cardinals WR Early Doucet (hamstring) is good to go, but it's a bench player, but you have to see. Someone has to step in and help relieve pressure on Larry Fitzgerald.
Patriots RB Danny Woodhead (ankle) is questionable, but has become a nonfactor, giving way to RB Stevan Ridley, drop it.
Falcons RB Jason Snelling (concussion) practiced this week and the game will be. The value falls Jacquizz Rodgers. Both are just backups and depth fantasy lists.
Bills RB CJ Spiller (knee) practiced on Friday and the game will be, but not worth a roster spot out of the big leagues. It belongs on your bench until he shows something.
Packers WR Donald Driver (shin) practiced on Friday and the game will be, but it's just a break in the week or injury fill-in the major leagues since the torch has passed to Jordy Nelson.
Raiders WR Louis Murphy (groin) was limited in practice this week and is questionable.
Seahawks TE Zach Miller (knee, ankle) and is likely to play but has no fantasy value.
Please feel free to send if you have any questions regarding this post , you can contact on
Disclaimer: The views expressed on this site are that of the authors and not necessarily that of U.S.S.POST.


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I hate '0' messenges Stamp by MephonixWatch
©2011-2017 Mephonix
I bet there are MANY people out there who hate having "0" Messenges....
I know i do... especially when im bored.
I know i do... especially when im bored.
same lol
so me DX
Then you get a +1 feedback... and it's a Llama
I'm bored with no messages.
This is totally me
Soo me!
that's why I keep a few XD
I know.
I know where I belong now.
☆*:.。. o(≧▽≦)o .。.:*☆ i hate when this happens so much
So true. XD
i alway keep one comment, but that specific comment means something to me tough
Awww that's so sweet ^_^
I hate it to >n<
I hate when I'm hating not having any messages...
Then I get one...
And its a picture from a group.
Then I get one...
And its a picture from a group.
Hah! I already stopped watching my groups. There is NO way I am handling more than a 100 deviations with limited time.
Me too! I feel so bored and unnoticed. DX
i hate it toooooooooooooooooooooooooooooooooooo
i do too
Same here..
Tick Tock Tick Tock...
Tick Tock Tick Tock...
Same here....especially when your idol who has a dA doesn't message you back...
*currently has 177* Pffft oh yeah I hate that too. XDDDD
I so very hate that.
I hate that to
i do to! i have 0 also. that just means my friends aren't on...crud.
Jonathanthesmex: is the link supposed to go to my gallery or is there something wrong here?
That link just goes to each persons own gallery. (It tricked me too.) LOL!
ohhhhhhhh...I suspected that, but my artwork's pretty bad, so I wouldn't have benn suprised. Now I'm kind of wondering how that works.
The horror!
Me right now!!! D:
I like comments the most
0 messages= UNACCEPTABLE!!!
I would love to have 0 lmfao. xD
I do... mainly because this is my hangout spot.
I have 0 message.
I have 0 message.


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The goal of the national taimen conservation campaign is to bring about greater awareness of and pride in Hucho hucho taimen (taimen) on a national level in Mongolia, leading to behavioural change to protect taimen and its habitat. With governmental and non-governmental partners, and through generous funding from the Disney Worldwide Conservation Fund we built on taimen conservation efforts in small watersheds (the Eg-Uur, Delger and Onon) by expanding the messaging and conservation practices broadly, using these tools:
· The regular advice and guidance from a project advisory team of American and Mongolian scientists, outfitters, and government and non-government representatives.
· Pre- and post-campaign surveys with anglers and other community stakeholders to measure behavioural change to protect the species.
· A media campaign using a campaign logo, presentations, distribution of educational materials developed during the campaign, and television broadcast. News stories include those in the Mongolian Daily News [national newspaper, September 2013], in Khentiin Medee [local newspaper, July 2013], and Facebook at "The Taimen Fund" and "Mongolian Fly Fishing Association."
· Signage in international airports, and poster distribution in soums (towns)
· Engagement with fishing clubs and distribution of taimen campaign hats with logos
· Building partnerships with local governments, ministries and schools to celebrate taimen.
The campaign hinged on a core campaign statement: "We believe in the conservation and sustainability of taimen, the pride and symbol of Mongolia's pristine nature, and in the people who have potential for conservation wisdom." This slogan, printed outreach materials, and posters were distributed to rangers, local protection agencies, organizations and community members broadly in the following areas:
· Arkh Province: Undur-Ulaan, Jargalant, Chuluum, Ikh Tamir and Tsetserleg soums
· Uvurkhangai Province: Kharkhorin
· Bulgan Province: Teshig, Khutag-Undur, Orkhon, Selenge soums and Khantai bagh
· Khuvsgul Province: Tsagaan-Uur, Renchinlkhubme, Tsagaan-Nuur, Ulaan-Uul, Tumurbulag, Rashaant, Erdenenbulgan, and Bayanzurkh soums
· Dornod Province: Bayan-Uul and Khalkh Gol soums
· Central Province: Mungun Morit and Erdene soums
· Khentii Province: Batshireet, Binder, Bayan-Adragan. Dadal and Norvolin soums
· Selenge Province: Eruu, Orkhon, Mandal, Bauangol and Tushig soums
· Cities: Darkhan, Erdenentm Baga-Nuur, Murun, Bulgan, and Zuunkharaa
The Taimen Fund is currently seeking funds to continue to build this campaign. Click here to view the report of the 2013 campaign outcomes.


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This year Ladbrokes decided to invite a selection of (quite amazing) bloggers from around Doncaster to famous and extravagant day that is, St Legers Ladies Day. I was absolutely ecstatic to get an invite and decided to take my dear Mother along with me as she's just turned 51 (do you like how I slipped that in, mum?) as she has never been to the races! I would love to say that the day was perfection from start to finish, but alas, my curse caught up with me (I'm paranoid) and we ended up having a tyre blow out just as we were ten minutes away. Thankfully, we were driving slow enough for nobody to get hurt, and the RAC man was good looking enough to lift my spirits, yet still embarrass me on twitter (you'll have to see a screen cap to understand that one).When we finally arrived, Victoria and Beth from Branded 3 came over to sit with us and two gentlemen from Ladbrokes, who ended up laughing at my mum and her hilarious comments all day. Not long after arriving we were ordering delicious food and relaxing in the amazing atmosphere. We were treated in the luxurious Hospitality Suite in the Mallard Restaurant where there were several private tables with balconies that had the most incredible view. We were fortunate to be exactly across from the race finish line! The ladies from Branded 3 made my mum and myself feel so comfortable and as if we were amongst friends, whilst the beautiful food and friendly service made the entire experience incredible. Getting ready for Ladies Day isn't as stress free as I would have hoped. However, after reviewing the ChiChi London Green Dolly Dress a couple months ago, I thought it would be the perfect opportunity to get my wear from it, and let it have a day out!
(Credit to Big Fashionista's Handy Boyfriend for this lovely picture, a selfie wasn't quite cutting it for how excited I was to be there!)
My Mum decided to tactfully hint about how good she looked in my new Joanna Hope Jersey Dress from Simply Be, which obviously meant that she had every intent in wearing it….
What we thought was going to be a 'buffet', ended up being a beautiful three course meal that was cooked to absolute perfection and presented like nothing I have ever seen before. I decided to go 'all out' and have pigeon, lamb and then a trio of desserts. However, my inner blogger was hoping my Mum would order something different so I could get a little variety, so the lovely gentlemen from Ladbrokes took some rather Instagram worthy photos of their desserts for me too!
One of my favourite bloggers and people, Kellie from Big Fashionista also attended the day with her hilarious and lovely boyfriend, which made Ladies Day even better. Seen as we were so excited to be seeing each other again after first meeting in January at the House of Fashion event, we of course marked the occasion with a mini photo shoot which resulted in the most hilarious photos. Including one where I was shouting at Kellie for implying she was faking her love for me, it obviously hurt me a lot because I made a rather unattractive face….
Half way through the day, and having lost every race so far, my Mum decided it was time to shake up her tactics and headed off outside to inspect the horses herself. We all laughed at the thought, but she actually ended up coming first that race! It left us all a little gobsmacked and we soon started listening to her expertise, even the gentlemen from Ladbrokes were interested in hearing what she had to say, which left me in fits of giggles. Especially at this one photo….
As the fabulous day was coming to an end, we got back to our tables to find a selection of sweet treats, including scones and little tiny pots of jam (if you're like me, little miniature things excite you immensely).
After admiring a group of Ladies hats from afar for pretty much all day, the ladies from Branded 3 dared me to go get their picture. After a little coaxing I finally got up to find a group of rather intoxicated, but nevertheless, friendly and well dressed women who were more than happy to have their photo taken!
Definitely aiming for my hat to be this fabulous if I ever go again!
The day, after we finally got there safely, was absolute perfection. Even the goodie bags from Ladbrokes and Branded 3 were one of the most thoughtful goodie bags I've ever received, as there were highlighters for the Race Day Bingo, good luck charms, nail varnish, flip flops, a camera, lip balm and even a mini whiteboard! (Below is a picture of the goodie bag from the official Ladbrokes Race Day Blog Post).
A big thank you to Branded 3 and Ladbrokes for inviting my Mum and me and for being such amazing hosts. I cannot recommend the hospitality suite enough as it is truly beautiful and it makes a day to remember.