text:
Upstream / Downstream
Explore pathways related to this product.
To Purchase # 14993S
|100 µl (10 western blots)
Find answers on our FAQs page.
PhosphoSitePlus® Resource
- Additional protein information
- Analytical tools
MLKL (D2I6N) Rabbit mAb #14993
Western blot analysis of extracts from various cell lines using MLKL (D2I6N) Rabbit mAb. KARPAS cell Line source: Dr. Abraham Karpas at the University of Cambridge.Learn more about how we get our images
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human MLKL protein (hMLKL-Myc/DDK; +), using MLKL (D2I6N) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).Learn more about how we get our images
Gallery: MLKL (D2I6N) Rabbit mAb #14993
Western Blotting Protocol
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
A. Solutions and Reagents
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
- 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
- 10X Tris Buffered Saline (TBS): (#12498) To prepare 1 L 1X TBS: add 100 ml 10X to 900 ml dH2O, mix.
- 1X SDS Sample Buffer: Blue Loading Pack (#7722) or Red Loading Pack (#7723) Prepare fresh 3X reducing loading buffer by adding 1/10 volume 30X DTT to 1 volume of 3X SDS loading buffer. Dilute to 1X with dH2O.
- 10X Tris-Glycine SDS Running Buffer: (#4050) To prepare 1 L 1X running buffer: add 100 ml 10X running buffer to 900 ml dH2O, mix.
- 10X Tris-Glycine Transfer Buffer: (#12539) To prepare 1 L 1X Transfer Buffer: add 100 ml 10X Transfer Buffer to 200 ml methanol + 700 ml dH2O, mix.
- 10X Tris Buffered Saline with Tween® 20 (TBST): (#9997) To prepare 1 L 1X TBST: add 100 ml 10X TBST to 900 ml dH2O, mix.
- Nonfat Dry Milk: (#9999).
- Blocking Buffer: 1X TBST with 5% w/v nonfat dry milk; for 150 ml, add 7.5 g nonfat dry milk to 150 ml 1X TBST and mix well.
- Wash Buffer: (#9997) 1X TBST.
- Bovine Serum Albumin (BSA): (#9998).
- Primary Antibody Dilution Buffer: 1X TBST with 5% BSA; for 20 ml, add 1.0 g BSA to 20 ml 1X TBST and mix well.
- Biotinylated Protein Ladder Detection Pack: (#7727).
- Prestained Protein Marker, Broad Range (11-190 kDa): (#13953).
- Blotting Membrane and Paper: (#12369) This protocol has been optimized for nitrocellulose membranes. Pore size 0.2 µm is generally recommended.
- Secondary Antibody Conjugated to HRP: Anti-rabbit IgG, HRP-linked Antibody (#7074).
- Detection Reagent: SignalFire™ ECL Reagent (#6883).
B. Protein Blotting
A general protocol for sample preparation.
- Treat cells by adding fresh media containing regulator for desired time.
- Aspirate media from cultures; wash cells with 1X PBS; aspirate.
- Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice.
- Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).
- Heat a 20 µl sample to 95–100°C for 5 min; cool on ice.
- Microcentrifuge for 5 min.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#13953, 5 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
- Electrotransfer to nitrocellulose membrane (#12369).
C. Membrane Blocking and Antibody Incubations
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
I. Membrane Blocking
- (Optional) After transfer, wash nitrocellulose membrane with 25 ml TBS for 5 min at room temperature.
- Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
II. Primary Antibody Incubation
- Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the product datasheet) in 10 ml primary antibody dilution buffer with gentle agitation overnight at 4°C.
- Wash three times for 5 min each with 15 ml of TBST.
- Incubate membrane with Anti-rabbit IgG, HRP-linked Antibody (#7074 at 1:2000) and anti-biotin, HRP-linked Antibody (#7075 at 1:1000–1:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
- Proceed with detection (Section D).
D. Detection of Proteins
Directions for Use:
- Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST.
- Prepare 1X SignalFire™ ECL Reagent (#6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.
- Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Western Blot Reprobing Protocol
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
- Wash Buffer: Tris Buffered Saline with Tween® 20 (TBST-10X) (#9997)
- Stripping Buffer: To prepare 100 ml, mix 6.25 ml of 1M Tris-HCl pH 6.8, 10 ml of 20% SDS and 700 μl β-mercaptoethanol. Bring to 100 ml with deionized H20. Make buffer fresh just prior to use.
B. Protocol
- After film exposure, wash membrane four times for 5 min each in TBST. Best results are obtained if the membrane is not allowed to dry.
- Incubate membrane for 30 min at 50°C in stripping buffer (with slight agitation).
- Wash membrane six times for 5 min each in TBST.
- (Optional) To assure that the original signal is removed, wash membrane twice for 5 min each with 10 ml of TBST. Incubate membrane with LumiGLO® with gentle agitation for 1 min at room temperature. Drain membrane of excess developing solution. Do not let dry. Wrap in plastic wrap and expose to x-ray film.
- Wash membrane again four times for 5 min each in TBST.
- The membrane is now ready to reuse. Start detection at the "Membrane Blocking and Antibody Incubations" step in the Western Immunoblotting Protocol.
posted June 2005
revised October 2016
|Western Blotting
MLKL (D2I6N) Rabbit mAb recognizes endogenous levels of total MLKL protein. This antibody also cross-reacts with an unidentified protein of 130 kDa in some cell lines.Species Reactivity: Human
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLKL protein.
Necroptosis, a regulated pathway for necrotic cell death, is triggered by a number of inflammatory signals including cytokines in the tumor necrosis factor (TNF) family, pathogen sensors such as toll-like receptors (TLRs), and ischemic injury (1,2). The process is negatively regulated by caspases and is initiated through a complex containing the RIP1 and RIP3 kinases, typically referred to as the necrosome. Mixed lineage kinase domain-like protein (MLKL) is a pseudokinase that was identified as downstream target of RIP3 in the necroptosis pathway (3,4). During necroptosis RIP3 is phosphorylated at Ser227, which recruits MLKL and leads to its phosphorylation at Thr357 and Ser358 (3). Knockdown of MLKL through multiple mechanisms results in inhibition of necroptosis (3-5). While the precise mechanism for MLKL-induced necroptosis is unclear, some studies have shown that necroptosis leads to oligomerization of MLKL and translocation to the plasma membrane, where it effects membrane integrity (6-9).
1. Christofferson, D.E. and Yuan, J. (2010) Curr Opin Cell Biol 22, 263-8.
2. Kaczmarek, A. et al. (2013) Immunity 38, 209-23.
3. Sun, L. et al. (2012) Cell 148, 213-27.
4. Wang, Z. et al. (2012) Cell 148, 228-43.
5. Wu, J. et al. (2013) Cell Res 23, 994-1006.
6. Cai, Z. et al. (2014) Nat Cell Biol 16, 55-65.
7. Chen, X. et al. (2014) Cell Res 24, 105-21.
8. Wang, H. et al. (2014) Mol Cell 54, 133-46.
9. Dondelinger, Y. et al. (2014) Cell Rep 7, 971-81.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Tween is a registered trademark of ICI Americas, Inc.


text:
Garland Brushed Grey Iron Sun Mirror
Stylish and elegant, those are two words that best describe this Garland Brushed Grey Iron Mirror. Perfect for your living room space, kitchen, hallway or bedroom, the mirror features a circular shape with leaves made from gorgeous iron surrounding it.
It has a very luxurious vintage look to it so you can easily use it as a focal point for your room or space. The design itself is also easy to pair with existing home decor and furniture. There's no need to worry about it looking out of place at all. The best part is that the subtle greyish colour also makes this easy to blend in with your existing interior colours.
Materials Used: MDF, Iron, Mirror
High quality construction
Beautiful design
Stylish and elegant
Modern/Classical design
Height: 70 cm
Width: 70 cm
Depth: 7 cm
Weight: 2.8 kg
Product information
- 4331
- Mirrors
- Viva Lagoon
Delivery and returns
This item is sold and dispatched directly by Viva Lagoon.
|Delivery cost
|Mainland UK - standard delivery
We offer a 14 day no-quibble money-back guarantee, so if you're not delighted with your purchase you can return it for a refund. Note that bespoke and made-to-order items are excluded.
About the seller
Viva Lagoon was started by Khira Benchekor and Edmund Eldergill and is committed to offering contemporary furniture that exhibits the best in modern design. More about Viva Lagoon...
Guaranteed and secure
- 14 day no-quibble returns *
- 100% safe and secure payments

subset: commoncrawl


text:
Contact Info / Websites
Recent Movie Reviews
Rated 5 / 5 stars
I enjoyed it and read the page on the book :3
LinkTCOne responds:
Ragville - Ep 1-1
Rated 4 / 5 stars
The opening really drew me in. However it should be noted that the music seemed a bit too loud, to the point that it went over the voices. The music itself also felt a tad awkward at times. Really liked the opening though.


text:
Mar 17 2017 - 5:45am
My husband is currently receiving SSA benefits. Started in January at age 66. I am 65 years of age. I was told by the SSA in Chicago that I should wait until I am 66 and claim under spousal and my benefits will grow until I am 70. Is this true? I thought SSA did away with this provision. Thanks
Yes, it's true. People born prior to January 2 1954 can still file for spousal benefits only at age 66 and let their own retirement benefit rate grow until age 70 (https://www.ssa.gov/planners/retire/deemedfaq.html). This may very well be the best option for you, but you might want to run the maximization software available on this website to be sure.
Best, Jerry


text:
Browse by Month
Extreme Events
- Department:August 1, 2017
Sure it's hot, but is it record hot? This map shows the hottest summer daytime high temperature recorded at thousands of U.S. stations prior to this summer.
- July 20, 2017
Heavy summer monsoon thunderstorms caused deadly flash floods in Arizona.
- Department:July 17, 2017
Heat waves bring some level of discomfort to nearly everyone. When excessive heat catches vulnerable populations off guard, that discomfort can advance to illness and even death. Public health officials in Minnesota are working to protect people in both rural and urban settings.
- July 13, 2017
An early summer heat wave in Europe broke records and helped spur wildfires across the region.
- June 28, 2017
Scorching heat impacted the western United States during the middle of June with record-breaking heat occurring from Arizona to the Pacific Northwest.
- Department:June 26, 2017
April showers bring May flowers, but what does June bring? History says mid-to-late June brings a higher probability of severe weather across much of the contiguous United States.
- Department:June 21, 2017
How do this year's temperatures on the first day of summer compare to historical highs and lows? Compare your local conditions to these maps showing the warmest and coldest first days of summer for more than 4,000 U.S. locations.
- June 12, 2017
Heavy rain in the first part of June has put a significant dent in the recent drought conditions in Florida.
- June 9, 2017
In late May, cyclone Mora made landfall across Bangladesh, a country vulnerable to cyclones and sea level rise.
- Department:May 31, 2017
Between the weekly forecast and a seasonal outlook—and often less accurate than both—sit "sub-seasonal" weather predictions. In this profile, atmospheric scientist Libby Barnes talks about her work leading a NOAA task force whose members are trying to overcome the special challenges of forecasting over the 2-week to 2-month horizon.


text:
Livonia, Michigan (PressExposure) September 28, 2009 -- "Aliaga Foundation" is having a fundraiser to help us raise money for the poorest children of Peru and Latin-America. This fun filled event is to bring together Hispanic people to mix and meet with Community Leaders from South Eastern Michigan at Laurel Manor in Livonia.
$50. Per person donation at the door / cash bar / food provided
ALIAGA FOUNDATION'S goal in Latin America and the Caribbean is to reduce extreme poverty, particularly in rural areas, by providing its microfinance institutions and other poverty-focused organizations with funding, technology support and management tools and strategies. This allows these organizations to expand their outreach to more families and increase their impact. Aliaga Foundation is working with Andeas, native communities in Peru and Latin America to improve education and their serious needs.
Our foundation has started a plan for sponsoring scholarship programs at universities and educational institutions in Latin-America, as we introduce American Universities to Latin-American Universities to become partners in international education. Aliaga Foundation, has agreements with Andina University of Cuzco, Continental University of Huancayo, Universidad Villarreal that we will support with books, educational supplies and we also introduced them to U of M - Flint and Wayne State University and have agreements with benefits for both countries and their students.
This year Jose Aliaga went to rural places in the Jungle and drove for over 14 hours to get into some of the poverty stricken areas where they don't have educational support. Aliaga Foundation bought all their educational supplies for 400 children, mostly pencils, paper and books. We then signed agreements with the poor communities that don't have modern systems of life that we will help with.


text:
Final configuration:
The SR 99 tunnel will be approximately two miles long. It will feature two 11-foot travel lanes in each direction, bordered by an eight-foot safety shoulder and a two-foot shoulder. Long, gentle curves will allow for safe sight distances. The southbound lanes are stacked above the northbound lanes, so drivers will not see oncoming traffic.
The tunnel will be built to modern earthquake standards. There will be no on- or off-ramps between the two tunnel portals. In the event of an emergency, exits every 650 feet provide shelter and escape routes, while a state-of-the-art safety and ventilation system assists first responders, and fights fire and fumes. (Read more about the tunnel's safety systems in the program library.)
Current progress
Last updated: Dec. 29, 2016
At the time crews stopped for the holidays in December 2016, Bertha had tunneled 6,787 feet of her 9,270-foot journey. The machine had placed 1,035 of the 1,426 rings that constitute the new SR 99 tunnel. You can track Bertha's progress on the Follow Bertha page.
Behind Bertha, crews build the interior structures that will carry northbound and southbound traffic. The walls and decks are built in sections, which allows crews space to work, provides concrete with time to cure, and increases the overall efficiency of the effort.


text:
The DM tip jar has runneth over. You guys have been far more generous than we dared to hope. We've used this bounty to pick up non-AL adventures, other content from the GM's guild, digital versions of maps, content for Fantasy Grounds that the store can use, and replaced some of the ungodly amount of printer ink AL consumes. However, since most of that doesn't benefit you guys directly, we wanted to make sure that you got something back too. To that end, we're making up cards to slide into some plastic name tents we have found.
If you are interested in one (or possibly more depending on how many people want one) then fill out this form. You can also share some ideas for other rewards that we can give out in the future.


text:
In-home visits for D'Angelo Harrison started this week and the four-star shooting guard from Fort Bend (Texas) Dulles hosted two major schools and two more will come in next week.
Marquette and Oklahoma State stopped by to visit Harrison in the last few days and Franchize All-Stars AAU coach Darrell Bogan said things have been going well. Baylor and St. John's have in-home visits set up for next week and then Harrison will have a better understanding of which program he likes most.
One of the most important things, Bogan said, is that Harrison would like at least a chance to play early so that's something he'll be looking for when talking to the coaching staffs.
Article Continues Below
"From what I gathered, they were really impressed with (Marquette) and they liked what Marquette was talking about," Bogan said.
"Dealing with D'Angelo, it's going to be a thing where he's looking for a place that fits him. It went well but at the end of the day he has to say is this the place where I can play as a true freshman? Will I get to play 25-30 minutes or am I going to be a role player if so-and-so gets hurt?"
Rivals.com rates Harrison, a 6-foot-3, 186-pound four-star prospect, as the ninth-best shooting guard in the class and No. 41 in the 2011 class.
Alabama, Arizona State, Arkansas, Houston, SMU, UTEP and Virginia have also offered but Harrison, who was fantastic at times in Las Vegas this summer, likes his chances with the four programs that have set up in-home visits.
"He's a scoring two-guard," Bogan said. "He may look undersized but he knows how to get to the basket. He's not the quickest guy but he knows how to get his shot off. He has unbelievable range and I think he can play anywhere he chooses to go. It just depends on the situation."


text:
The Magnetic RF Bottle Tag provides secure anti-theft protection for a wide variety of bottled wine and spirits. This versatile hard tag features an adjustable multi-strand stainless-steel reinforced strap that attaches to assorted bottle neck sizes for a strong visual deterrent. Its small form factor does not interfere with brand promotion or product merchandising, while its intuitive design is quickly and easily applied and removed, helping improve in-store operations.
Copyright 2015 || Barcode Vault


text:
Stanley Whitney
Stanley Whitney
Stanley Whitney: Six Paintings
First Minute of a Dream, 2009
Whitney's paintings are geometric in nature, but reflect a deeper understanding about color and touch and are highly concerned about the human relationship to the nature of paint and color theory itself. Being extremely sensitive to the method of how paint is applied to the canvas and whether or not a specific color is repeated twice throughout a painting, Whitney's work is extremely intuitive. He sees color as magical, placed on the canas based on feeling and emotion. His painting background is based in drawings of abstract landscapes, and moved towards color-field esque style paintings after a trip to Egypt in 1994. Whitney's work also relates to his identity as an African-American man living among the white-dominated art world.
"Red is dominant. Red, yellow, blue, green. The palette just comes. I follow my work wherever it goes—out the door, around the corner. I gotta follow the work. It's high key now because I'm focusing on intensity. It's kind of a heavy beat—it comes from the West African drumbeat. Music in the African-American community is what saves people. I grew up and music was everything. That's a big part of my painting."
- Stanley Whitney, 2008
Stanley Whitney was born in Philadelphia, PA in 1946. He received his BFA from the Kansas City Art Institute, and later received his MFA from Yale University in 1972. He has shown his paintings in galleries and institutions throughout the world, some including the Albert Baronian Gallery in Brussels, Christine König Galerie in Vienna, Jack Shainman Gallery in NY, and Barbara Greene Gallery in Miami, FL to name a few. He has won the First Robert De Niro Sr. Prize in Painting in 2011, and the Academy Award from the New York Academy of Arts and Letters in 2010. He lives and works in New York and Parma, Italy.
Installation view, from left to right: Agean (2009), Blue Meets Yellow (2011), and Osawatomie Brown (2010).
First Minute of a Dream, 2009
Installation view, from left to right: Champagne and Lion (2010) and Cezanne's Mountain (2010).
Installation view, from left to right: First Minute of a Dream (2009), Champagne and Lion (2010), and Cezanne's Mountain (2010).


text:
Mundial Ham Slicer
Item Added to Cart
Loading Description..
Get everything you need in one go
Loading...Store pick up is not available for this product. Please order online to have this delivered.
Delivery estimate to (change):
Please select a product option to continue.
Usually ships in 3 - 5 business days
Bring the power of a fully forged knife into your kitchen with the Mundial 26cm Ham Slicer. The Mundial Knife offers a quality blade, well built handle and the expertise of more than 70 years of gourmet knife experience.
Quality Blade
The blade of the Mundial Slicer is made from high carbon stainless steel, and will easily and cleanly slice through your ham.
Well Built Handle
The Mundial Slicer has a full tang, polyacetal handle that is not only functional and comfortable to hold, but also looks wonderfully modern with three rivets.
|Product Type
If you feel your product has failed a Consumer Guarantee (as defined by Australian Consumer Law) then don't worry you can easily return it to your local Harvey Norman store. We'll even pay for the postage if you live in an area where there is no store. Our returns policy covers any purchased item (conditions apply).
Either complete the details below or chat to a staff member online.
Like this? Share it Share this on Facebook Share this on Google+ Share this on Twitter Share this on Pinterest


text:
Intention to designate the old MATIC building (Dewan Tunku Abdul Rahman) as a heritage site must be fulfilled
Media Statement by Dr. Ong Kian Ming, Member of Parliament for Serdang, on the 19th of March, 2017
Intention to designate the old MATIC building (Dewan Tunku Abdul Rahman) as a heritage site must be fulfilled
On the 8th of December, 2016, the Commissioner of Heritage published a public notice to de-gazette the national heritage status of three plots of land on the Malaysian Tourism Center (MATIC) which is located along Jalan Ampang in Wilayah Persekutuan (See Appendix 1 below). This public notice raised many concerns including the president of the Badan Warisan Negara, Elizabeth Cardosa.[1] Some feared that the Dewan Tunku Abdul Rahman (currently known as the MATIC building), where Malaysia's first Agong was installed in 1959 and where the Malaysian parliament first met in 1959, would be demolished and go the way of the Bok House.[2]
I put in a question to the special chamber of parliament during the ongoing parliament sitting, specifically asking about the status of the MATIC building and I received an official reply from Datuk Seri Nazri Aziz, the Minister of Tourism. In his reply, the Minister stated that the reason why the three plots of land were degazetted as heritage sites was because only one of the plots namely Lot 139 Section 58 has heritage value, namely the Dewan Tunku Abdul Rahman building. In his reply, the Minister also stated that the public notice to state the government's intention to designate this building as a heritage site has already been published on the 26th of January 2017 (See Appendix 2 below).
The Minister also gave me his verbal assurance that any construction that will take place in the plot of land adjacent to the Dewan Tunku Abdul Rahman would be in line with the conditions stipulated in the National Heritage Act 2005 including having the necessary buffer zone as a protection against the heritage site.
I welcome this decision by the Minister to ensure that an important building in Malaysia's history in the heart of Kuala Lumpur will be preserved and protected under the National Heritage Act 2005. As citizens who care about the history and heritage of our nation, we must continue our efforts to protect important heritage sites not just in Wilayah Persekutuan but all over the country as well. In the meantime, we will be waiting for the publication of the public notice confirming that Dewan Tunku Abdul Rahman has been officially been designated as a heritage site (since the public notice on the 26th of January only states the intention of declaring this building as a heritage site).
Dr. Ong Kian Ming
Member of Parliament for Serdang
Appendix 1: Public Notice degazetting three plots of land on the Malaysian Tourism Center (MATIC) as a heritage site
Appendix 2: Federal Government Gazette stating the intention of designating the Dewan Tunku Abdul Rahman as a heritage site
Further References:
- Parliamentary Reply on degazetting of heritage sites: 1, 2 and 3
- P.U. (B) 57 Notice to Designate MATIC as a Heritage Site
[1] http://www.nst.com.my/news/2017/01/202296/status-revocation-not-provided-law?d=1
[2] https://cilisos.my/for-the-first-time-in-history-malaysian-heritage-buildings-are-going-to-be-de-heritaged/
Why is Menara Warisan allowed to be built on the site of Taman Merdeka Negara when the Tourism Minister has confirmed that it is within the buffer zone of Stadium Merdeka and Negara and is protected under Section 25 (1) of the National Heritage Act 2005?
Media Statement by Dr. Ong Kian Ming, MP for Serdang and Fong Kui Lun, MP for Bukit Bintang on the 10th of October, 2013 in Kuala Lumpur
Why is Menara Warisan allowed to be built on the site of Taman Merdeka Negara when the Tourism Minister has confirmed that it is within the buffer zone of Stadium Merdeka and Negara and is protected under Section 25 (1) of the National Heritage Act 2005?
Dr. Ong asked a question in the recent parliamentary sitting on why Section 25 (1) of the National Heritage Act 2005 was not used to gazette Taman Merdeka Negara / Tunku Park, which is next to Stadium Merdeka and Stadium Negara, as part of the national heritage site.
Tourism Minister Dato' Seri Mohamed Nazri Abdul Aziz's reply, on the 30th of September, was that Taman Merdeka is already under the buffer zone for Stadium Merdeka and Stadium Negara under Section 40(3)(a) of the National Heritage Act. In addition, even though Taman Merdeka Negara is not gazetted as a national heritage site because it does not meet the conditions under Section 67(2), it is already protected under Section 25(1). We quote his reply in BM:
'Untuk Makluman Yang Berhomat Serdang, Taman Merdeka Negara telah pun termasuk sebagai Zon Penampan kepada Stadium Merdeka dan Stadium Negara di bawah Section 40(3)(a), Akta Warisan Kebangsaan 2005. Sungguhpun Taman Merdeka Negara tidak diwartakan sebagai tapak warisan kerana tidak menepati kreteria di bawah Seksyen 67 (2), ia sudah pun dilindungi di bawah Seksyen 25 (1).'
Section 40 of the National Heritage Act states that:
(1) The Commissioner shall coordinate and advise the local planning authority before any planning permission or development order is granted involving a heritage site.
(2) (c) measures that have been taken to secure the safety of the heritage site and the neighbouring land; and
(3) (a) any land adjoining within a distance of two hundred metres from the boundary of the land to which an application under this section relates;
Section 25 (1) of the National Heritage Act states that:
Where a site has no natural heritage or cultural heritage significance but the Commissioner is satisfied that it should be designated as a heritage site because of its proximity to and for the protection and enhancement of another site designated as a heritage site under section 24, the Commissioner may so designate such site as a heritage site.
Since Taman Merdeka Negara is within 200 meters of Stadium Merdeka and Negara, we want to know if the Commissioner of Heritage, Professor Emeritus Datin Paduk Siti Zuraina Bt Abdul Majid, has consulted DBKL on the possible negative impact of the building of Menara Warisan on these two National Heritage Sites. We urge the Commissioner to request that DBKL not proceed with the development order that reports indicate have already been given so that construction can begin on Menara Warisan.
We also urge the Commissioner, with the full support of the Minister of Tourism to designate Taman Merdeka Negara as a Heritage Site in order to protect and enhance Stadium Merdeka and Negara under Section 25 of the National Heritage Act.
Dr. Ong Kian Ming, MP for Serdang
Fong Kui Lun, MP for Bukit Bintang
Question for the Minister of Tourism and Culture
Answer from the Minister of Tourism and Culture, Dato Seri Mohamed Nazri Abdul Aziz (Taman Merdeka Negara)


text: The nation's capital has a mass transit system plagued with problems of nearly every kind. And now comes the revival (very expensive) of the streetcar system. Reason TV provides a brief survey of the history of DC streetcars: By: Redactor Video: How Today's Fascists Do It Video: Less Oppression, Not Equal Oppression Leave a Reply Cancel reply Your email address will not be published. Required fields are marked *Comment Name * Email * Website − = Notify me of follow-up comments by email. Notify me of new posts by email.


text:
Fully Licensed & Bonded
This 2 star hotel is located in the city centre of Paris. It is close to the Sacre-Coeur and the nearest station is Porte de Clignancourt. The Hotel has a restaurant, a bar, a conference room and a coffee shop. All 49 rooms are equipped with hairdryer and safe.


text:
Wednesday, March 31, 2010
Got up for work this morning, dazed, in bathroom I notice it is still dark. Stagger back to bedroom, examine clock again. 3AM. *sigh deeply*
Monday, March 29, 2010
Is it just me or is it BLOODY FREEZING?
Sunday, March 28, 2010
Take away curry on a Sunday evening, this is a wonderful thing.
Friday, March 26, 2010
Bit of rain...
Still amazed how drunk I can get purely by drinking bitter.
Thursday, March 25, 2010
Wednesday, March 24, 2010
Wondering how I can get a job proofing scam emails. 'I hereby wish to crave your indulgence'. Now, personally, I'd phrase that differently.
Tuesday, March 23, 2010
Stilton breath. Come 'ere and give us a kiss darlin'...
Discovery of the day: Taking 15mg of Dihydrocodeine before a 10 minute nap actually results in a 1 hour hallucinatory sleep. With snoop dog.
Monday, March 22, 2010
Oh no, run out of shower gel. Rummages in cupboard. Blows dust off old box: DAVIDOFF Cool Water Shower Gel... Thanks and RIP Nan.
Sunday, March 21, 2010
Wondering if Guinness is the answer?
Eggs ingested, stage #2: journey to the tip commences... The beer stage (#3) seems somewhat remote.
Saturday, March 20, 2010
Friday, March 19, 2010
Waiting for my future, time-travelling self to appear and whisk me six hours forward... I appear to be late.
Uuuurgh, need... more... sleep...
Thursday, March 18, 2010
Kaeng kua bamboo shoot curry
Very hot! Bit heavy on the bamboo. The recipe calls for a lot of chicken, I used mushroom instead, but perhaps need more or something different next time - tofu or mock meat perhaps?
Sauce is coconut based.
Posted by Ralph Capper at 4:51 AM No comments:
What a day! I actually have the back door open.
Wednesday, March 17, 2010
Considering how nice a pint of Guinness would taste right about now...
Tuesday, March 16, 2010
Monday, March 15, 2010
Back causing considerable pain. A long swig of cool, refreshing milk turns out to be off - spat over kitchen floor. This day starts badly.
Sunday, March 14, 2010
I've felt better.
Saturday, March 13, 2010
Friday, March 12, 2010
Typical. I find a top I like in Tkmaxx and it turns out it isn't theirs, it's a shoplifter-swap!
Just rescued Enki the cat from the Quarry. Again. Silly beast.
Thursday, March 11, 2010
Wednesday, March 10, 2010
Tuesday, March 09, 2010
If what you want is a hot bath, then there's little worse than a cold one.
Monday, March 08, 2010
Trying to fix an error I can't reproduce. Such challenges often appear on Mondays.
I still don't think Mondays are very funny.
Sunday, March 07, 2010
Feeling the guilt that is brought on by sitting inside your house, looking out the window at the bright sunshine.
Amazing alcohol drunk several hours ago is making me sigh, as I review the evening.
Saturday, March 06, 2010
Found myself back at the beer festival...
Up and at em!
Friday, March 05, 2010
Uuuuurrrgh, brains.... I need brains....
Thursday, March 04, 2010
It's Beer Festival Time!! :D Anyone going to the Lancaster Beer Fest tonight, tomorrow, Sat? 7pm onwards... Oooh, I love a beer festival....
Tuesday, March 02, 2010
Monday, March 01, 2010
I need a holiday. In the sun. For six months. Or more, I'm not fussy.
Subscribe to: Posts (Atom)


text:
Real Estate in Allenhurst, GA
Allenhurst is located in Georgia. Allenhurst, Georgia has a population of 665. Allenhurst is less family-centric than the surrounding county with 30.18% of the households containing married families with children. The county average for households married with children is 35.55%.
The median household income in Allenhurst, Georgia is $46,250. The median household income for the surrounding county is $43,209 compared to the national median of $53,482. The median age of people living in Allenhurst is 39.5 years.
Allenhurst Environment
The average high temperature in July is 92.4 degrees, with an average low temperature in January of 40.3 degrees. The average rainfall is approximately 48.2 inches per year, with 0.1 inches of snow per year.
Allenhurst Map
Allenhurst Real Estate offices
Real Estate near Allenhurst
Nearby Cities
Allenhurst Zip Codes
Nearby Cities Homes for Sale


text:
My Nonprofit Reviews
Review for Toledo Community Foundation, Toledo, OH, USA
Great Foundation for the community. Everyone should work with the Toledo Community Foundation. It is a netural body that sets about solving the community's problem through grants as well as working donor to create funds that give back to the community.
More Feedback
I've personally experienced the results of this organization in...
programs through out the community that the Foundation help to start or keep going over the years
What I've enjoyed the most about my experience with this nonprofit is...
Open to helping in any way they can. A true leader in the community.
The kinds of staff and volunteers that I met were...
Everyone from their President to the staff representatives in the community.
If this organization had 10 million bucks, it could...
Make it group to 100 million and support the community for many lifetimes to come.
When was your last experience with this nonprofit?


text:
SONAR is a technique used for detecting objects through sound propagation. There are two types of SONAR systems: active SONAR systems and passive SONAR systems.
In an active sonar system, a pulse of sound is created using a SONAR projector consisting of an electroacoustic transducer or array, a power amplifier, and a signal generator that propagates to the target and produces an echo. The time taken by the echo to return is then measured to determine the location of the object.
In a passive SONAR system, the target is a source of energy that creates a pulse of sound to produce an echo, which is then analyzed to determine the location of the object.
The analysts forecast global sonar system market to grow at a CAGR of 7.31% during the period 2016-2020.
Covered in this report
The report covers the present scenario and the growth prospects of the global SONAR system market for 2016-2020. To calculate the market size, the report considers the revenue generated from the sales of SONAR systems to individual customers, enterprises, and government organizations.
The market is divided into the following segments based on geography:
- Americas
The report, Global SONAR System Market 2016-2020, has been prepared based on an in-depth market analysis with inputs from industry experts. The report covers the market landscape and its growth prospects over the coming years. The report also includes a discussion of the Key vendors operating in this market.
Key vendors
- Kraken Sonar
- Kongsberg Maritime
- Raytheon
- Thales
- Teledyne Reson
Other prominent vendors
- Harris
- Northrop Grumman
- R2Sonic
- Sound Metrics
- Neptune SONAR
- Garmin
- Furuno Electronic
- Johnson Outdoors
- Navico
- FLIR Systems
Market driver
- Focus on improving safety of marine transportation
- For a full, detailed list, view our report
Market challenge
- Adverse effect on marine life
- For a full, detailed list, view our report
Market trend
- Mergers and acquisitions
- For a full, detailed list, view our report
Key questions answered in this report
- What will the market size be in 2020 and what will the growth rate be?
- What are the key market trends?
- What is driving this market?
- What are the challenges to market growth?
- Who are the Key vendors in this market space?
- What are the market opportunities and threats faced by the key vendors?
- What are the strengths and weaknesses of the key vendors?


text:
@Drakan wrote:
….. claim on a bank guarantee for a breach of contract for say 150.000 Euros, that comes out of the bank's coffer incidentally not the developer's.
Drakan – I stand totally corrected then on that one. Bernard and mg, you were both right 😳
@Drakan wrote:
You seriously cannot expect to walk in a bank and claim on a bank guarantee for a breach of contract ……
Sadly, this isn't the case otherwise why would you need to pay a lawyer to do this when it can be so easily achieved ?
Drakan, with respect – I have never intimated the above as a procedure to claim on a Bank Guarantee.
Having been through it myself, I know very well there is a correct procedure (via one's lawyer), obtaining the correct documentation, making the appropriate requests to developer/bank via burofaxes etc. and the required time-scale that these events involve.
I would never advocate trying to claim on a BG without a lawyer on-side handling the whole legal procedure – it's worth every penny the lawyer's costs involve. 😉


text:
Bow Ties by "Bertie Squillini"
For Gentlemen who "Dare to be Different"
A Range of Bow Ties in stunning and unique colours and designs
So many people have commented that our Doggie Bow Ties are good enough for men! Well now thanks to the inspiration of you and our in house designer Mr "Bertie Squillini" (Yes our Wire Fow Terrier) we are pleased to be able to offer them for sale very soon. We plan to offer them in all our Coloured Designs. Made simply for Men that also "Dare to be Different"
Remember we are a fun brand and if we can va va voom your look on that up coming cruise or Christmas Ball then you will make us very happy indeed.
Coming Soon


text:
|Forex Tips For Beginners
Here's a bitter pill to swallow – to be successful you must accept that risk is part of the game. Yes, you will lose money in Forex, especially when first starting out. However, the goal is to gain sufficient knowledge and experience to help minimise risk and learn to win more often than lose. This can be a tough road and one fraught with pitfalls so be sure do study as much as you can about the Foreign Exchange.
The Forex market is open 24 hours per day starting in Syndey Australia and ending in New York. It's a highly volatile market but also the biggest liquid market in the world. As much as we all would love to find a money making system that requires next to no effort or knowledge, one that will bring in cash on auto-pilot with ease, this isn't the case with Forex so be warned.
Firstly, especially as a beginner, you need to develop a strategy. Learn how to follow trends based on observation and an understanding of the different currencies and markets. Avoid guessing, especially that which is based on emotions and what you think (or hope) the markets "might" do. This can cost you dearly. Seek out reputable experts and find quality software and websites.
Learn to study technical analysis and read up as much as you can about how to read and follow the charts. Take as much time as you need to feel confident about how the market works. Your efforts will pay off dividends if you are properly prepared and develop a strategy based on solid principles, not wild speculation.


text:
Angiogenesis and Vasculogenesis Are Impaired in the Precocious-Aging klotho Mouse
Jump to
Background— The effects of aging on angiogenesis (vascular sprouting) and vasculogenesis (endothelial precursor cell [EPC] incorporation into vessels) are not well known. We examined whether ischemia-induced angiogenesis/vasculogenesis is altered in klotho (kl) mutant mice, an animal model of typical aging.
Methods and Results— After unilateral hindlimb ischemia, laser Doppler blood-flow (LDBF) analysis revealed a decreased ischemic-normal LDBF ratio in kl mice. Tissue capillary density was also suppressed in kl mice (+/+>+/kl>kl/kl). Aortic-ring culture assay showed impaired angiogenesis in kl/kl mice, accompanied by reduced endothelium-derived nitric oxide release. Moreover, the rate of transplanted homologous bone marrow cells incorporated into capillaries in ischemic tissues (vasculogenesis) was lower in kl/kl mice than in wild-type (+/+) mice, which was associated with a decrease in the number of c-Kit+CD31+ EPC-like mononuclear cells in bone marrow and in peripheral blood. Finally, the 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor cerivastatin restored the impaired neovascularization in kl/kl mice, accompanied by an increase in c-Kit+CD31+ cells in bone marrow and peripheral blood, and enhanced angiogenesis in the aortic-ring culture.
Conclusions— Angiogenesis and vasculogenesis are impaired in kl mutant mice, a model of typical aging. Moreover, the age-associated impairment of neovascularization might be a new target of statin therapy.
Received August 7, 2002; de novo received January 14, 2004; revision received April 13, 2004; accepted April 14, 2004.
Postnatal neovascularization comprises angiogenesis and vasculogenesis and is regulated by a complex interplay among various growth factors, circulating bone marrow (BM)–derived precursor/stem cells, and inflammatory cells.1–3 Angiogenesis is defined as the formation of new blood vessels by way of sprouting of preexisting mature endothelial cells,1 whereas vasculogenesis is considered the creation of blood vessels by differentiation of endothelial precursor cells (EPCs).3,4
Aging is one of the important risk factors for arteriosclerosis. The occurrence of coronary artery disease and peripheral arterial occlusive disease increases with age, even in a population without other major risk factors.5,6 Recent studies have shown that aging impairs ischemia-induced neovascularization.7 However, little is known as to whether ischemia-induced neovascularization, especially vasculogenesis, becomes impaired with age, probably because of the paucity of appropriate animal models of aging.
Kuro-o et al8 recently developed klotho mutant (kl/kl) mice that show typical age-related phenotypes, such as arteriosclerosis, skin atrophy, osteoporosis, and infertility. The appearance of kl/kl mice is normal until 4 weeks after birth, after which they become inactive and die prematurely at 8 to 9 weeks.8 All of these phenotypes are caused by disruption of the single klotho gene, and interestingly, the klotho protein functions as a humoral factor, or "antiaging" hormone. In the present study, taking advantage of klotho mice, we investigated the effects of aging on ischemia-induced neovascularization, with a special focus on angiogenesis and vasculogenesis.
Heterozygous klotho mutant (+/kl) mice were generated as described previously.8 kl/kl and wild-type (WT; +/+) littermate mice were obtained by crossing +/kl mice.
Mouse Model of Angiogenesis
The Institutional Animal Care and Use Committee of Kurume University School of Medicine approved the experimental protocol. A mouse model of hindlimb ischemia was prepared as described previously.9 WT, +/kl, and kl/kl mice were subjected to unilateral hindlimb ischemia. Systemic blood pressure and heart rate were measured in the conscious state with a tail-cuff blood pressure analyzer (MK2000, Muromachi).
Laser Doppler Blood-Flow Analysis
At 6 time points (before and after surgery and on postoperative days 3, 7, 14, 21, and 28), we measured limb blood flow with a laser Doppler blood flow (LDBF) analyzer (MoorLDI, Moor).9 LDBF data were expressed as the ratio of ischemic-limb to normal-limb LDBF.9
Tissue Capillary Density
Additional mice (n=6 for each group) were euthanized by intracardiac injection of 30 mg pentobarbital sodium on day 28 after limb ischemia. Thigh adductor skeletal muscles of bilateral limbs were isolated, embedded in OCT compound, and snap-frozen in liquid N2. Multiple cryostat sections 5 μm thick were prepared and subjected to alkaline phosphatase staining to identify capillary endothelial cells.10
Aortic-Ring Culture for Angiogenesis Assay
Descending thoracic aortas were isolated from WT and kl/kl mice (n=3 from each group). Under a dissecting microscope, multiple 1-mm-thick aortic rings were prepared. Rings were then placed between 2 layers of growth factor–reduced Matrigel (Becton Dickinson) supplemented with medium 199, 20% fetal bovine serum, 10 U/mL heparin, antibiotics, and bovine pituitary extracts (Life Technologies).11 Microvascular sprouting was characterized by the incorporation of 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate–labeled acetylated LDL (DiI-acLDL).12 We measured the length of endothelial sprouts at 4 randomly selected points at right angles to each other and calculated the mean length.
Analysis of EPCs
We quantified EPC-like mononuclear cells (MNCs) in both peripheral blood (PB) and bone marrow (BM) after induction of hindlimb ischemia because EPCs mobilize from BM.13 WT and kl/kl mice (n=5 each) were subjected to limb ischemia and euthanized on day 14 to collect PB (0.2 mL) and BM cells. PB-MNCs were isolated by centrifugation through a Histopaque-1083 density gradient. BM cells were obtained from the bilateral femurs by flushing the BM cavity with heparinized saline.14 PB-MNCs and BM cells were suspended in phosphate-buffered saline containing 1% bovine serum albumin and were subjected to flow cytometry.
EPC-like MNCs were identified as c-Kit+CD31+ or CD34+CD31+ cells.14 PB-MNCs or BM cells were incubated with a fluorescein isothiocyanate–conjugated anti-mouse c-Kit monoclonal antibody (clone 2B8, Immunotech) or with a fluorescein isothiocyanate–conjugated anti-mouse CD34 monoclonal antibody (PharMingen), followed by phycoerythrin-conjugated anti-mouse CD31 monoclonal antibody (clone MEC13.3, PharMingen). Cells were finally fixed with 1% paraformaldehyde (pH 7.5) in phosphate-buffered saline and analyzed by flow cytometry.
In additional WT and kl/kl mice (n=5 each), BM cells were isolated as described earlier. BM-MNCs were then obtained by centrifugation through a Histopaque density gradient, and cells (7×105) were subjected to culture assay in 2% gelatin–coated, 12-well plastic dishes to quantify the number of differentiated EPC-like attaching cells.15
Homologous BM Cell Transplantation
Donor WT and kl/kl mice (n=10 each) were subjected to hindlimb ischemia and were euthanized on day 14 to collect BM cells. Donor BM cells were labeled with the green fluorescent marker PKH2-GL (Sigma).15 Recipient WT and kl/kl mice (n=10 each) were also subjected to unilateral hindlimb ischemia, and they received BM cell transplantation through the tail vein (1.4 to 1.8×107 cells per mouse) on the day of surgery. Each recipient mouse received PKH2-GL–labeled BM cells isolated from 1 donor mouse. On day 14, the recipient mice were euthanized, and ischemic skeletal muscles were harvested. Tissues were embedded in OCT compound and snap-frozen in liquid N2. Five-micron-thick sections were stained with either an anti–von Willebrand factor (vWF) monoclonal antibody (clone 4F9, Immunotech) or an anti-CD31 monoclonal antibody (clone MEC13.3) followed by a tetramethylrhodamine isothiocyanate–conjugated secondary anti-IgG1 (Jackson ImmunoResearch) or anti-IgG2a (Nordic Immunological Laboratories) antibody, respectively, to detect endothelial cells by fluorescence microscopy. We calculated the percentage of PKH2-GL+CD31+ or PKH2-GL+vWF+ double-positive cells among the population of CD31+ or vWF+ endothelial cells as the BM cell incorporation (BMCI) ratio with the following formula: BMCI ratio (%)=number of PKH2-GL+CD31+ cells (or PKH2-GL+vWF+ cells)/total number of CD31+ cells (or vWF+ cells)×100. Five microscopic fields (×40 magnification) were examined from samples of 6 animals in each group, and the mean BMCI ratio was calculated.
Urine NOx and Tissue cGMP Content
We measured urinary excretion of NOx (nitrite [NO2−] plus nitrate [NO3−]) in WT and kl/kl mice to examine whether systemic nitric oxide (NO) production was decreased in kl/kl mice. NOx was measured by high-performance liquid chromatography after reduction of total NOx to NO2−.16
Thigh skeletal muscles were harvested from ischemic hindlimbs of WT and kl/kl mice (n=5 from each group) on day 14. Samples were weighed, snap-frozen in LN2, and stored at −80°C until biochemical analyses of tissue cGMP were performed.16
Effects of Statin Therapy on Neovascularization in kl/kl Mice
An additional 15 kl/kl mice were treated with cerivastatin (Bayer), a lipophilic 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor (statin).17 These animals were administered cerivastatin (5 mg · kg−1 · d−1) for 28 days by subcutaneous injection on the dorsal skin starting on the day of surgery. The dose of cerivastatin, which had been determined in a previous study, effectively enhanced vascular endothelial NO synthase activity and induced vasodilatation in mice.17 We measured the ischemic-normal hindlimb LDBF ratio and the capillary density. Serum total cholesterol levels were measured with a commercially available kit (Wako). The ex vivo aortic-ring culture assay was also performed to examine whether ex vivo angiogenesis was altered in cerivastatin-treated kl/kl mice. Finally, we determined the number of c-Kit+CD31+ EPC-like MNCs in BM and PB-MNCs in the cerivastatin-treated animals.
Data are expressed as mean±SE. Differences among the 3 groups were analyzed by 1-way ANOVA followed by Scheffe's comparison for 2 groups. Differences between the WT and kl/kl mice were analyzed by unpaired Student's t test. The comparative incidence of limb amputation was evaluated by χ2 test. Probability values <0.05 were considered statistically significant.
Unilateral Hindlimb Ischemia in klotho Mutant Mice
After the left femoral artery and vein were excised, the ratio of ischemic (left) to normal (right) hindlimb blood flow decreased to comparable levels (0.09 to 0.11, P=NS) in all 3 groups, blood pressure and heart rate did not differ among the 3 groups during the experimental period.
Ischemic-Normal Hindlimb Blood Flow Ratio
The data in Figure 1A show the trends in hindlimb LDBF, which disclose a progressive recovery of LDBF in the ischemic limb of WT mice (Figure 1B). In contrast, LDBF in kl/kl mice remained impaired throughout the follow-up period, and the ratio was persistently lower in kl/kl mice than in WT mice (P<0.001, Figure 1B). In heterozygous +/kl mice, the LDBF ratio was lower than that of WT mice; however, it was greater than that of kl/kl mice (n=10 each). Therefore, the degree of impairment of the LDBF ratio was related to the severity of allele mutation of the klotho gene (LDBF ratio: +/+>+/kl>kl/kl; Figure 1B).
Tissue Capillary Density
The results in Figure 1C are photomicrographs of alkaline phosphatase–stained skeletal muscles with or without ischemia. Capillary density in ischemic hindlimbs, as examined on day 28, was significantly reduced in kl/kl mice and in +/kl mice compared with WT mice (Figure 1C and 1D). Quantitative analysis revealed that the degree of reduced capillary density was related to the severity of allele mutation of the klotho gene (capillary density: +/+>+/kl>kl/kl; Figure 1D). In contrast, the capillary density in nonischemic hindlimbs examined on day 28 did not differ among the 3 groups (Figure 1D, n=6 each).
Aortic-Ring Culture Assay for Ex Vivo Angiogenesis
The findings in Figure 2A are photographs representing microvascular sprouting from cultured aortic rings. Sprouted vessels incorporated DiI-acLDL, indicating that the sprouts were endothelial cells.12 On day 7 of culture, quantitative analysis revealed that the mean length of the vessels sprouted from the edge of aortas was significantly shorter in kl/kl mice than in WT mice (n=3 each, Figure 2B and 2C). Thus, ex vivo angiogenesis was impaired in kl/kl mice compared with WT mice.
Tissue cGMP Contents and Urinary NOx Secretion
To examine whether the klotho mutation affected NO production in the ischemic hindlimbs, we examined the tissue contents of cGMP. The tissue cGMP level was significantly lower in kl/kl mice than in WT mice. Also, the amount of urinary NOx secretion, which indirectly represents endothelium-derived NO formation, was significantly lower in kl/kl mice than in WT mice (n=5 each, Figure 2D).
EPCs in BM and in PB-MNCs
On day 14 after hindlimb ischemia, we analyzed EPC-like MNCs in BM and PB by flow cytometry. EPC-like MNCs were identified as c-Kit+CD31+ or CD34+CD31+ MNCs. The numbers of c-Kit+CD31+ cells and CD34+CD31+ cells in both BM and PB were significantly lower in kl/kl mice than in WT mice (n=5 each, Figure 3A and 3B). BM-MNC culture assay further revealed that the number of differentiated EPC-like attaching cells was significantly lower in kl/kl mice than in WT mice (Figure 3C).
BM Transplantation for Vasculogenesis Analysis
We examined whether intravenously transplanted homologous PKH2-GL–labeled BM cells were incorporated into vascular structures in ischemic tissue. Histological analysis on day 14 revealed that PKH2-GL+ BM cells were incorporated into microvascular structures in ischemic tissues, and most of the cells were also stained for either CD31 (Figure 4A) or vWF (Figure 4B), markers of endothelial cells. The number of PKH2-GL+ cells per microscopic field was significantly lower in kl/kl mice (receiving kl/kl BM cells) than in WT mice (receiving WT BM cells, Figure 4C and 4D). The number of CD31+ or vWF+ capillary endothelial cells was also significantly lower in kl/kl mice than in WT mice. The incorporation rate of transplanted BM cells into capillary structures as assessed by the BMCI index was significantly lower in kl/kl mice than in WT mice (Figure 4C and 4D). LDBF analysis showed a decreased ischemic-normal hindlimb blood flow ratio on day 14 in kl/kl mice compared with WT mice (data not shown).
Effects of Cerivastatin on Neovascularization in kl/kl Mice
After hindlimb ischemia, LDBF analysis showed that the ischemic-normal hindlimb LDBF ratio was significantly greater in cerivastatin-treated kl/kl mice than in kl/kl mice (Figure 5A and 5B). On day 28, capillary density was significantly greater in cerivastatin-treated kl/kl mice than in untreated kl/kl mice (Figure 5C and 5D). Microvascular sprouting from aortic rings was also greater in cerivastatin-treated kl/kl mice than in untreated kl/kl mice (Figure 6A and 6B). These changes were accompanied by an increased tissue cGMP content and increased number of c-Kit+CD31+ EPCs in BM and PB after cerivastatin treatment (Figure 6C and 6D). Serum levels of total cholesterol and triglycerides did not differ among the WT, kl/kl, and kl/kl-cerivastatin groups (data not shown), indicating that cerivastatin did not change the ischemia-induced neovascularization by altering the serum lipid profile.
Limb Amputation Ratio
After hindlimb ischemia, spontaneous limb amputation occurred in only 3.1% of 32 WT animals. Among kl/kl mice, however, spontaneous amputation occurred in 24% of 25 animals. Cerivastatin treatment resulted in significant limb salvage in kl/kl mice, and autoamputation of the ischemic limbs occurred in only 6.3% of 48 tested animals (P<0.05 vs untreated kl/kl mice, Figure 7A and 7B).
In the present study, in vivo ischemia-induced neovascularization was impaired in klotho mutant mice, and both ex vivo angiogenesis and in vivo vasculogenesis were also inhibited in klotho mice. Interestingly, cerivastatin treatment rescued the impaired ischemia-induced neovascularization in kl/kl mice.
Because our aortic-ring organ culture assay can avoid the contribution of EPCs to neovascularization, the data indicate that angiogenesis is impaired in kl/kl mice. There may be several mechanisms for the impaired angiogenesis in kl/kl mice. We previously showed that angiogenesis depends on endothelium-derived NO.9 Furthermore, endothelium-derived NO lies downstream of vascular endothelial growth factor–induced angiogenesis and plays a central role in endothelial migration,18,19 an essential process for angiogenesis. Because endothelium-derived NO production declines with age and because endothelium-dependent vasorelaxation is impaired in klotho mice,20,21 the insufficient endothelium-derived NO formation may at least partly account for the impaired angiogenesis in kl/kl mice. Consistently, the tissue cGMP levels, a downstream molecule of NO, and urinary NOx secretion were reduced in kl/kl mice in the present study.
We next examined whether vasculogenesis was impaired in kl/kl mice. Circulating EPCs mobilize from BM and are incorporated into angiogenic foci.13 Because BM function declines with age,22 it is conceivable that the number, mobilization, and/or function of EPCs would be decreased in kl/kl mice. Vasa and coworkers23 recently showed that the migratory property of EPCs was impaired by the presence of coronary risk factors, including aging. However, the effects of aging on the numbers of EPCs have not been well examined previously. In the present study, the numbers of EPC-like c-Kit+CD31+ as well as CD34+CD31+-MNCs were significantly decreased in both BM and PB in kl/kl mice. Thus, EPC differentiation and/or EPC mobilization from BM is likely impaired in kl/kl mice.
The mechanism by which the number of EPCs is decreased in kl/kl mice remains to be determined. A recent study indicated that the klotho protein regulates the BM microenvironment, including macrophages, fibroblasts, endothelial cells, and extracellular matrixes, and the 3-dimensional structure composed of these cells is an important environment for hematopoiesis and EPC differentiation.24 A deficiency of the klotho gene has also been reported to cause osteoporosis,8 which further disturbs the BM microenvironment.24 Taken together, the reduced number of EPCs in kl/kl mice might be caused at least partly by alterations of the BM microenvironment.
We then performed homologous BM cell transplantation studies to further clarify in vivo vasculogenesis. The incorporation rate of BM cells into capillary structures (BMCI ratio) was significantly reduced in kl/kl mice. Therefore, it is possible that ischemia-induced vasculogenesis is also inhibited in kl/kl mice. The mechanism of the reduced BM cell incorporation into capillaries is currently unknown. However, a recent study showed that vascular endothelial growth factor could mobilize EPCs from BM and induce EPC homing to ischemic tissues.25 Because ischemia-mediated vascular endothelial growth factor induction decreases with age,26 the decreased vasculogenesis may be explained in part by a decreased vascular endothelial growth factor formation in kl/kl mice. This issue should be further examined.
Because "antisenescence" is one of the major topics of recent biomedical research,27 we explored a potential therapeutic strategy against the impaired neovascularization in kl/kl mice. In the present study, cerivastatin accelerated recovery of the LDBF ratio and increased capillary density in kl/kl mice. Cerivastatin also increased tissue cGMP levels, indicating recovery of the tissue NO-cGMP pathway. Consistently, ex vivo angiogenesis in aortic-ring culture was restored in cerivastatin-treated kl/kl mice. Cerivastatin also increased the number of c-Kit+CD31+ MNCs within both BM and PB. The latter findings are consistent with the results of recent reports showing that statins not only enhance angiogenesis but also stimulate the mobilization of EPCs from BM.28 Therefore, cerivastatin likely augmented both angiogenesis and vasculogenesis in kl/kl mice, resulting in increased limb blood flow and significant limb salvage in kl/kl mice.
There is a limitation in the present study. It is still unknown whether our findings were caused by the klotho gene mutation per se or secondarily by the aging phenotype of kl/kl mice. Currently, the precise role of the klotho protein in terms of antisenescence efficacy is not well known. Abnormalities in calcium/phosphorus metabolism have been postulated in kl/kl mice,27 but its role in reduced angiogenesis is still unknown. Nevertheless, the present study demonstrates that the klotho gene product may function as a maintenance factor for endothelium-derived NO, EPC differentiation and/or mobilization, and thus, neovascularization in response to tissue ischemia.
In summary, our findings have several clinical implications. First, reduced neovascularization with age could contribute to the increased incidence and severity of ischemic peripheral/heart diseases in the elderly.5,6 Second, age-related impairment of neovascularization could be rescued by statins. Because statins have been reported to inhibit some age-associated disorders (eg, osteoporosis and dementia),29,30 it would be intriguing to test whether statins could rescue impaired neovascularization in elderly patients.
This study was supported by grants from the Ministry of Education, Science and Culture and from the Ministry of Health and Welfare of Japan, the Tokyo Biochemical Research Foundation, and the Terumo Foundation. We thank K. Kimura, K. Moriyama, and M. Aoki for technical assistance.
- ↵
- ↵
- ↵
- ↵
Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997; 275: 965–967.
- ↵
- ↵
- ↵
Rivard A, Fabre J-E, Silver M, et al. Age-dependent impairment of angiogenesis. Circulation. 1999; 99: 111–120.
- ↵
- ↵
- ↵
- ↵
- ↵
Burbridge MF, West DC. Rat aortic ring: 3D model of angiogenesis in vitro. In: Murray JC, ed. Angiogenesis Protocols. Totowa, NJ: Humana Press; 2001: 185–204.
- ↵
- ↵
- ↵
- ↵
- ↵
Sata M, Nishimatsu H, Suzuki E, et al. Endothelial nitric oxide synthase is essential for the HMG-CoA reductase inhibitor cerivastatin to promote collateral growth in response to ischemia. FASEB J. 2001; 13: 2530–2532.
- ↵
- ↵
Murohara T, Witzenbichler B, Spyridopoulos I, et al. Role of endothelial nitric oxide synthase in endothelial cell migration. Arterioscler Thromb Vasc Biol. 1999; 19: 1156–1161.
- ↵
Murohara T, Yasue H, Ohgushi M, et al. Age related attenuation of the endothelium dependent relaxation to noradrenaline in isolated pig coronary arteries. Cardiovasc Res. 1991; 25: 1002–1009.
- ↵
- ↵
- ↵
- ↵
Okada S, Yoshida T, Hong Z, et al. Impairment of B lymphopoiesis in precocious aging (klotho) mice. Int Immunol. 2000; 12: 861–871.
- ↵
- ↵
Rivard A, Berthou-Soulie L, Princepe N, et al. Age-dependent defect in vascular endothelial growth factor expression is associated with reduced hypoxia-inducible factor 1 activity. J Biol Chem. 2000; 275: 29643–29647.
- ↵
- ↵
Vasa M, Fichtlscherer S, Adler K, et al. Increase in circulating endothelial progenitor cells by statin therapy in patients with stable coronary artery disease. Circulation. 2001; 103: 2885–2890.
- ↵
Fassbender K, Simons M, Bergmann C. et al. Simvastatin strongly reduces levels of Alzheimer's disease β-amyloid peptides Aβ42 and Aβ40 in vitro and in vivo. Proc Natl Acad Sci U S A. 2001; 98: 5856–5861.
- ↵
This Issue
Jump to
Article Tools
- Angiogenesis and Vasculogenesis Are Impaired in the Precocious-Aging klotho MouseToshifumi Shimada, Yoshiaki Takeshita, Toyoaki Murohara, Ken-ichiro Sasaki, Kimiyasu Egami, Satoshi Shintani, Yosuke Katsuda, Hisao Ikeda, Yo-ichi Nabeshima and Tsutomu ImaizumiCirculation. 2004;110:1148-1155, originally published August 30, 2004https://doi.org/10.1161/01.CIR.0000139854.74847.99
Citation Manager Formats
Share this Article
- Angiogenesis and Vasculogenesis Are Impaired in the Precocious-Aging klotho MouseToshifumi Shimada, Yoshiaki Takeshita, Toyoaki Murohara, Ken-ichiro Sasaki, Kimiyasu Egami, Satoshi Shintani, Yosuke Katsuda, Hisao Ikeda, Yo-ichi Nabeshima and Tsutomu ImaizumiCirculation. 2004;110:1148-1155, originally published August 30, 2004https://doi.org/10.1161/01.CIR.0000139854.74847.99Permalink:


text:
Re: Upp, Bentz, Hahn,Knertzer, Hartley
In reply to:
Re: Upp, Bentz, Hahn,Knertzer, Hartley
I don't know if any of the following opens doors, but perhaps the Knortzer/Upp and Sprinkle families were associated if not related:
The Knortzer (Knertzer) family resided in the Manchester communities, as did the Upp and Sprenkle families. (source:
The Pennsylvania Original Land Records for York County (Manchester townships)
3--Sprenckle,Maria Elizabethbaptz. 3-9-1760b.Manchester Twn d.>1823
s-Upp,Jacobm.York Co PA c1778 b.York Co PA 1751 moved to Louisville KY
4--Upp,Jacob jr.
s-Roberts, G. H. judge
(source: http://www.geocities.com/charlescuy/sprenkel.htmlhttp://www.geocities.com/charlescuy/sprenkel.html)
Knertzer and Upp listed in Will:
Jacob UPP - will was probated April 18, 1767 by the following: Peter BENTZ of Dover Twp - Tavern
Keeper, Philip BENTZ of York - Town Yeoman, and Michael HAHN of York - Blacksmith.
One Jacob Upp is connected to Knertzer (Balthasar Knortzer).(note Balthasar Knortzer family listed in will)
Jacob UPP's will was written May 19, 1758. Executors of the will were sons Jacob and Peter UPP--------
see also
Do you know which Jacob Upp is in
Isacc Knight Indian Capture lists: John and Jacob Upp's capture?


text:
|1 | 2 | 3 | 4 | 5 | Next >>
|1 of 14
Lot 5/ 4 McCarthy Street, Port Augusta West, SA 5700
3 Bed | 2 Bath | 3 Car
Property ID: SA6837318
This is the second magnificent double storey townhouse we have been given the privilege of selling in this group of 6. The home has generous open style living areas, the kitchen boasts stainless steel Blanco kitchen appliances, timber cupboards and hand crafted glass bi-fold doors open onto a lovely alfresco area with views of Spencer Gulf and the Baxter Ranges. The master bedroom has walk-in robe, the 2nd and 3rd bedrooms both have built-ins and the 2 bathrooms are simply stunning. A cozy living room with great views is located upstairs. Ducted reverse cycle air conditioning, fully paved driveway, stainless steel balustrades, double garage UMR and ample storage space complete this executive home.
View CENTURY 21 Top of the Gulf (Port Augusta)
Contact Agent:
Gregory Edwards
Mobile: 0427092503
Email: Email Me


text:
The Moscow summit between the U.S. and USSR in Moscow, Russia. Wyeth family artwork being packed in boxes for a visit to the Soviet Union. Various paintings being packed up. In exchange, some Russian paintings and artwork seen that will be displayed in America. General Secretary of the Communist Party of Soviet Union Mikhail Gorbachev visits the United States during Glasnost efforts. People on the streets in what appears to be New York or possibly Washington DC are surprised when he exits his motorcade to shake hands directly with American citizens gathered on the sidewalks.The U.S. Secretary of State George Shultz and his wife visiting Soviet Georgia and being greeted by Soviet citizens. Gorbachev and U.S. President Ronald Reagan side by side at a summit in Washington DC. They stand together on the White House lawn as a band plays. Animation depicts the sharing of cultures between the two nations and the exchange of magazines. View of American rock star Billy Joel and his band performing at a concert in the Soviet Union on New Year's eve. A Moiseyev Dance Company folk troupe from Russia performing a dance in the United States. Cultural exchange between the two countries includes views of a piano performance by Vladimir Horowitz in Moscow and a ballet performance in the United States by the Bolshoi Ballet. Several Russian school children pose for pictures on the steps of the Lincoln Memorial in Washington DC. Soviet teenage children enjoy a elevated ropes course in an outdoor program. People sit in an amphitheater with officials at a Chautaqua Talk in 1987. A group of Soviet citizens view a touring "Information USA" exhibit in the Soviet Union that presents American culture. They view the program on an array of television monitors. A young Russian boy speaks in Russian about his interest in learning about American culture. People watch a science exhibition. Astronauts of the USA and Soviet Union in a spacecraft. The astronauts open a connecting hatch and shake hands. Narrator describes collaboration of the two nations to monitor and study the depleting ozone layer of the earth and how to protect it. A man is shown sunbathing. Officials of the America and the Soviet Union sign an agreement relating to sharing information about safety of nuclear power plants. Soviet representatives and George Shultz, the U.S. secretary of State, inaugurate a new communication system that communicates nuclear dangers between the two countries. A woman in the communication room. Food grain pouring from a harvester, part of what is supplied to the Soviets. Soviet Union tanks and soldiers in Afghanistan. Soviet soldiers walking on a street in Afghanistan in front of a shop. Arrested American journalist Nicholas Daniloff disembarks an aircraft after his release. View of the exterior of the American Embassy in Russia. U.S. marine Sgt. Clayton J. Lonetree being escorted under guard after having been accused of collaborating in a Soviet spy mission at the U.S. embassy in Moscow. Scene inside a Voice of America radio broadcast studio. Sign indicates it is broadcasting in Russian. Close up of meter moving on a radio instrument during the broadcast. Dancers from the Dance Theatre of Harlem performing before their visit to the Soviet Union. A group of Soviet citizens including men, women and children walking outdoors. Mikhail Gorbachev with U.S. President Ronald Reagan at a ceremony on the White House grounds on December 8, 1987.
The US-USSR Moscow Summit on Human rights in Moscow, Soviet Union. U.S. president Ronald Reagan speaks on issues relating to Human rights. Other officials seated in front. He speaks about the right to immigrate and travel. View of the Kremlin in Moscow. U.S. Secretory of State George Pratt Shultz speaks about strategic arms reductions in light of the weapons race. The White house press secretary Marlin Fitzwater at a press conference. The board reads 'Department of State'. Exteriors of the building. View of protesters permitted to demonstrate in the Soviet Union indicating some improvement in conditions. In Washington DC, citizens march on streets demonstrating for the freedom of Soviet Jews. The soviet scholar Peter Reddaway speaks about the reforms and highlights reforms and changing Soviet policy on open-ended internment of mental health patients. People pray inside a church in the Soviet Union. George Weigel, the president of the James Madison Foundation, speaks of importance of religious freedom in the Soviet union on a news panel. Weigel states that religious freedom is a key test of Glasnost and Perestroika. Exterior view of the Ukrainian Catholic National Shrine of the Holy Family under construction in Washington DC, built by Ukrainian American Catholics. People in the Soviet Union protest, marching on a street and holding signs and pickets. A large military parade in the Soviet Union in Red Square.
37th anniversary of Soviet Army and Navy celebrated on February 22nd at Central Soviet Army theater in Moscow. Exterior of 2nd Soviet Army theater. Soviet Army and Navy Marshals, Generals and Admirals attend this event and are seated in the theater. Presiding committee include Nikolai Bulganin, Nikita Khrushchev , Marshal Vasilevsky and other ministers. The meeting is opened by USSR Defense Minister, Marshal Georgy Zhukov, who congratulates Soviet Army and Navy on their 37th anniversary. Soviet Army and Navy were established by Congress party. The audience applaud. Speeches of Secretary of the Moscow City Committee of the communist party, Ivan Marchenko; by the Secretary of the Central Committee of the Young Communist League, and by Hero of Socialist Labor and chairman of the Lenin Collective Farm, Ivan Yubanov. At the end of the event, Marshal Zhukov delivers thanksgiving speech.
A crowd enjoys fireworks in Red Square, Moscow, Soviet Union, at night. Past events show Soviet Premier Joseph Stalin, U.S. President Franklin D. Roosevelt and Prime Minister Winston Churchill of the United Kingdom in Tehran, Iran for the Tehran Conference of 1943. U.S. President Harry S. Truman in Germany during the Potsdam Conference of 1945. The Grand Kremlin Palace in Moscow, Soviet Union. Soviet people along a street in Moscow. Foreign Minister of the Soviet Union, Vyacheslav Molotov with others applauding at the Bolshoi theater, in Moscow. Anti-soviet disorders. Street cars being overturned. (Hungary?).
Poland aids the Soviet Union during German-Russian Combat in the Union of Soviet Socialist Republics (USSR) during World War II. General Sikorsky of Poland is greeted by Vyacheslav Mikhailovich Molotov, Chairman of the Council of the People's Commissars of the Soviet Union at Stalingrad. General Sikorsky and the General Secretary of the Communist Party of the Soviet Union Joseph Stalin plan an agreement for mutual defense. Two flags flying from flag poles. Polish troops stand at attention. Soviet or Polish troops march. Soviet troops at battle stations in a field. Soviet tank corps men run out and jump into their tanks. A tank goes out through a city near Moscow. Battle action during winter shows Soviet troops advancing to meet on coming Germans. A large gun mounted on a railroad (RR) car. A Soviet officer gives a signal to fire guns. All types of artillery-guns mounted on RR cars, armored trains, tanks field artillery, large cannon fire. Several buildings being demolished by shell blasts. Tanks advance. The Soviets advance across snow covered terrain. Soviet civilians gather up German equipment and pile it around in the area. Dead Germans on the ground. Destroyed German mechanized equipment. Observation towers and lookout posts commanded by the Soviets.
Goosestepping German troops on the march and German Panzer I tanks moving in formation across a field in World War 2. A formation of German Fieseler Fi 156 Storch airplanes in flight overhead, accompanied by two He-111 bombers. German paratroopers jumping from Ju-52 aircraft. Grand Admiral Erich Johann Albert Raeder head of the German Navy, making a statement. Bucolic scenes of European farm country and vineyards, with local people harvesting crops. Soviet troops marching on parade to a military band on the sidelines in Red Square, Moscow, soviet Union. Soviet motorcycle troops armed with light machine guns. Soviet T-26 light tanks in formation. Glimpse of Red Square, with St. Basil's Cathedral and other famous buildings. Soviet troops crossing a river on inflated boats. A long column of T-26 tanks moving along a dirt road. Sky filled with variety of Soviet warplanes, including four-engine bombers (with fixed landing gear) to bi-wing aircraft. Soviet paratroopers jumping from a Tupolev ANT-6 bomber. They climb out on the huge corrigated wing and slide off. View out past the tail of an ANT-6 bomber. Soviet Union and Japan conclude a neutrality pact. Views of the pact being signed by Foreign Minister Vyacheslav Mikhailovich Molotov for the Soviet Union and by Japanese Foreign Minister Matsuoka and Ambassador Yoshitsugu, for the Japanese, in Moscow on April 13, 1941. Joseph Stalin is observing in the center of the scene.


text:
Join us in Columbus, Ohio for our 2014 region 7 NODA conference, ColumBUS: Your Ticket to Great Ideas! Conference highlights include educational sessions, keynote speakers, a community service project, case study competitions, and our renowned Closing Brunch Fashion Show. Climb aboard for your next great orientation and transition programs adventure!
NODA Region VII ConferenceColumBUS: Your ticket to great ideasJoin us in Columbus, Ohio for our 2014 region 7 NODA
conference, ColumBUS: Your Ticket to Great Ideas! We hope our
participants learn, engage, and network through our diverse educational
sessions for undergraduates, graduates, and professionals; through
participation in our community service project and case study
competitions for undergraduate and graduate students; and, through our
opportunities unique to region 7, including our Regional Orientation
Leader Institute (ROLI) and our Closing Brunch Fashion Show featuring
orientation attire and swag across our region. Attendees should indicate
their intent to participate in these experiences on their conference
registration form.
11/27/2017Indiana State Drive-In
12/8/2017New Jersey State Drive-In
12/13/2017Webinar: Writing for Publication as a Practitioner
12/15/2017Pennsylvania State Drive-In
12/15/2017Washington D.C. Drive-In
10/24/2017ACUI's Women's Leadership Institute
6/15/2017Where Respect Happens
2/10/20172015-2016 NODA Annual Report
1/19/20172016 Global Summit on Student Affairs and Services
1/4/2017NODA Annotated Orientation, Transition, and Retention Bibliography
2829 University Avenue, Suite 415 Minneapolis, MN 55414
Telephone: 612.301.6632 Toll free: 1.866.521.NODA (6632)
Fax: 612.624.2628 | Email Us >


text:
Read the latest buzz about Electrify Magazine:
"New York City–based Electrify Mag prove[s] that sometimes the best way to be transported to a new destination without actually going there is not by scrolling through hashtags or clicking on blog posts. [Electrify] hopes to attract a new kind of traveler: young, curious and looking for an adventure (even, or especially, if they don't have the resources to stay in five-star accommodations)." READ ›
"Something tells us, if we were to stand on top of a volcano – we still wouldn't look as smoking hot as Ashley Smith. The newly launched Electrify Magazine tapped the gap-toothed gal-next-door to grace the cover of Volume 01. Naturally, Electrify threw a party to celebrate the launch, where Smith and fellow Sports Illustrated model Hayley Clauson got down to beats provided by DJ Tennessee Thomas as they sipped on tequila." READ ›
"Thursday was action packed, beginning with the arrival of Ashley Smith's latest cover for the newly launched quarterly travel mag, Electrify. The supermodel flashed her signature gap-toothed smile with pals Hailey Clauson and the magazine's founder Amanda Ho at their blowout launch helf at Loft 29 in Chelsea." READ ›
"For the modern day social slayer, being a girl-about-town just won't cut it; we all want to be a girl-about-the-globe. Electrify Magazine is what we're reading to achieve that. The luxury quarterly's 'Global Generation' issue takes readers from a volcano in Nicaragua to a temple in Laos, inspiring hungry creatives to travel off the beaten path while staying true to a cultivated aesthetic." READ ›
"Electrify Magazine launched 'Global Generation' Volume 01 featuring model Ashley Smith. Electrify Magazine is a new luxury quarterly travel publication tailored for creative millennials who love to travel and embody a global perspective. Guests gathered at Loft 29 including Sports Illustrated model Hayley Clauson, DJ Jasmine Solano, rapper Leaf, singer Maad Moiselle, Rox Brown, Ian Mellencamp and more." READ ›


text:
Home page not displaying
Hi. I just installed WordPress 3.4.2 and am using the 2011 theme. I can create new pages and display them, but when I edit the home page and click the View Page button, it does not display. What does display is the old home page from prior to my WordPress Installation. Even if I use shortlink I'm taken to the old home page, and not the one created with WordPress. Also, if I view one of the new pages I created, and click the link to the Home page, it too takes me to the old page.
Thank you for any insight you can provide.
- The topic 'Home page not displaying' is closed to new replies.


text:
DINOSAUR ADVENTURE is essential for one Theme/Amusement Park. Looking below to see our dinosaur projects in different Theme/Amusement Patks around the world.
ONLYDINOSAURS PRODUCTION is deserved for cooperation by Theme/Amusement Parks in the world!
Do you want our Dinosaurs Alive to decorate your Theme/Amusement Parks? We customize for you only!
Customizing more Dinosaurs Alive for Theme/Amusement Parks!


text:
What is Chatbot and How It Could Transform Travel Industry?
What is Chatbot and How It Could Transform Travel Industry?
2016 has been the year we all came to know about chatbot. With the Facebook's announcement at its F8 developer conference in April 2016 that stated businesses now will be able to use chatbots to deliver automated customer support via 'Messenger' service, the word 'chatbot' gained international attention. Earlier in March, Microsoft introduced its chatbot called Tay. Let's see what is chatbot and how it works?
What is Chatbot?
Chatbots are micro applications that live inside chat app platforms like Facebook Messenger, Slack, and Telegram. Powered by artificial intelligence and machine learning capabilities, they can read and write message. The chatbot is essentially a user interface which can be plugged into a number of data sources via APIs, so it can deliver information for user queries in real time through automated appropriate text or voice support. Chatbot is capable for effectively mimic human conversation and react to spoken or written prompts.
How Chatbot Works?
Chatterbots recognize keywords in the users input and then access a database to give a predefined response. Let's say the user is giving the bot sentence that contains the word "dog". The chatbot is most likely to ask something like: "How your dog looks like?" or "Tell me more about the dog". Combining pre-set scripts and deep learning neural networks, bots can predict an accurate response to an asked question in a way that skips phases of conversation to mimic regular chat. The same question can be asked via voice and the answer will be delivered in the same manner.
How Chatbot Could Benefit Travel Industry?
As a service based industry, the travel industry achieves its success on the experience level of customers. Travel companies can leverage chatbots to enhance customers experience, through advanced and quicken customer support. Bots can be used to provide travellers a simple overview of flights and hotels available based on their criteria and booking option. A bot that can have both the travel data and destination content would become a perfect travel companion. The travel bot would provide travellers information about their journey step by step.
On the journey date, the bot would retrieve all the boarding information like time of the flight, boarding time, check point, terminal number and even more information about the airport.
The bot can also help travellers in knowing the following things:
• Nearest business lounge
• Baggage allowance
• Time taken to reach the airport
A travel bot not only consists of travel and accommodation information but also gives suggestion on most famous places to watch. This is very useful for a person who is not familiar with the destination. With these transformational benefits, bots have grabbed the attention of thousands of travel companies who want to achieve first-mover advantage in their field.
Chatbots are called next-generation technology, which are helpful not just in customer support or not just for travel industry, businesses of any sort can leverage chatbots for internal support, sales and advice strategies. By choosing a right mobile app development partner, travel companies can develop a great chatbot that can help them achieve a desired success.
Want to know how chatbot can help your business?
Please reach us at firstname.lastname@example.com or fill up the form to develop a chatbot. Let us provide you a free consultation.


text:
Final Fantasy XIV: Update 3.4 Soul Surrender Launching September 27
What was revealed:
- Update 3.4 will be released on September 27. New story, side quests, and the final part of the Alexander raid will be added. Besides, there will be two new trial, and two new dungeons
- Xelphatol and Gubal hard version of the Great Library.
- The PvP gets various changes. There will be custom games, dueling system and spectator mode.
- The Grand Companies will receive a new rank (1st Lieutenant)
- The Gold Saucer also recieves changes.
- Houses apartments and aquariums. And orchestrion will be available to the playlist.
- Players using the controllers can see the new variations in hotbar
- The game will attend TGS 2016
- in patch 3.45 Palace of the Dead Deep Dungeon to get new floors (51 to 200). And the new Anima weapon quests will be added.
You! +1


text:
Just In
June 2013 Issue
Eat Right to Fight Cancer
This year, more than 1.6 million men and women will be diagnosed with cancer, but the simple act of eating the right foods can help prevent, or even support your treatment of this devastating disease, according to the Academy of Nutrition and Dietetics. You can help reduce your risk with a few simple lifestyle strategies...
To continue reading this entire article you must be a paid subscriber.
Subscribe to Environmental Nutrition
Get the next year of Environmental Nutrition for just $20. And access all of our online content - how to fight heart disease, cancer, stroke, diabetes, osteoporosis, Alzheimer's, and dozens of other diseases - free of charge.
Already subscribe but haven't registered for all the benefits of the website? Click here.
Subscriber Log In
Forgot your password? Click Here.


text:
There are several different types of materials that are used for building the perfect staircases. Aluminum, wood and even steel are the usual choices for the Steel stair stringers manufacturers Brisbane. It mainly depends on the construction which sort of material will be used, and it is usually a matter of client's choice. Style and design are just two of the crucial factors that help determine which kind of materials should be used.
Steel stairs stringers are generally selected for the strength and durability provided. This is specially true when it is about placing them inside the public buildings as steel can take a great deal of abuse.
Designing options for steel stairs stringers vary between straight up and down or spiral to vertical and circular in style. It is the vertical style that is generally used in the public buildings, but this does not make the spiral design out of question as it gives a grand look and mostly placed at the entrance of plush luxury hotels. The wooden staircases are not to be left out though for the amazing beauty they bring. On the other hand, you need to keep this fact in mind that with the present time's technology even the steel made stair stringers can be easily constructed to look nearly identical to wood. Check this site out
When speaking of the steel stairs stringers, people generally consider that they are very complicated to install, but it is not necessarily so. There are just a few fundamental components used that can help these stringers put together. For industrial purposes you need to believe that steel is the best way to go. This is a fact as unlike the wooden stairs, steel stairs offer better reliability.
The strength that steel offers is incredible as it can bear very heavy loads with ease. The strength not just comes from the steel they are made from. But from the way the different components are bolted together. The engineering that goes into designing of these steel stair stringer structures is just part of what actually makes them so tough.
No stairs of any sort complete without handrails to offer the needed support for those climbing them. Without these safety devices it would be a lot easier for anyone to step right off from a high height. Nearly everyone who makes use of stairs also grasp the handrails for added safety.
With the right materials and designs to build quality steel stair stringers it is a lot easier to build durable, reliable, safe and beautiful way to get one from floor to another or from the pavement or ground into a building.
Views: 21


text:
Linda Kizzire
County Treasurer
p. (316) 660-9110
f. 316.383.7113
525 N. Main
Wichita, KS 67203
Contact the Treasurer's Office
2017 Foreclosure sales have been
held, check back for 2018 sale information or you can
register to receive notifications on the next sale
via email.
Sedgwick County's most recent
interactive mapping application,
Tax Foreclosure Auctions, makes
it easier for prospective bidders to investigate the
properties available for auction. The application can be
accessed on any mobile device.
In the state of Kansas, counties are
allowed to sell real property having delinquent real
estate taxes at the conclusion of the judicial tax
foreclosure procedure via open auction
(no sealed bids). Properties are sold to the highest
qualified bidder.
"Notice of sale" is published in the
officially designated county newspaper, the DERBY
INFORMER. Copies are available in the county treasurer's
office, first floor of the courthouse. When the date of
sale is set, the case can be accessed at on this
website. The Sedgwick County Treasurer's Office should
have Map Books for each sale available at a nominal
The Mission of Sedgwick County is to provide quality
public services to our community so everyone can
pursue freedom and prosperity in a safe, secure, and
healthy environment.
© Copyright 2014
See our Website Privacy Policy & Legal Disclaimer
and Notice of Privacy Practices Regarding Medical Information.


text:
01462 338189
We Believe its all About You
Hush Beauty is located in the market town of Hitchin, Hertfordshire.
We are dedicated to delivering the finest beauty therapies in a warm, friendly and inviting atmosphere where the most important consideration is "you".We are committed to the utmost standards of service and our focus is identifying your individual needs and delivering personalised treatments to ensure you get the most out of your experience. Talk to us now and let us show you what can be achieved with just a little bit of Hush.
Give the Gift of Hush…
Gift Vouchers can be purchased for any of our treatments or for any amount. Please call the salon on 01462 338189
and we can get your Hush Voucher delivered right to your door.
Click here for
Treatment Promotions


text:
[Elite_video_player id="39″]
DENVER — General manager Tim Connelly reaffirmed Thursday that the Nuggets still plan to re-sign restricted free agent Mason Plumlee this summer, despite an inconsistent second half of the season in Denver.
"He's a restricted free agent. We hold all the cards," Connelly said. "And we're looking to forward to getting something done with him this summer."
Plumlee, 27, was acquired from the Portland Trail Blazers for Jusuf Nurkic and the No. 19 pick in this summer's draft on Feb. 12 and appeared in 27 games for the Nuggets over the last three months of the regular season. The 29-year-old averaged 9.1 points and 6.4 rebounds in 23.4 minutes per game for Denver.
"I think Mason did a very good job," Connelly said. "Tough situation going from a starter to being a third big, fourth big at times. And it's always difficult when you're traded midseason."
Plumlee was brought to Denver to provide some consistency and continuity from the Nuggets' starting unit, to their bench. While Denver runs their first unit offense through Jokic, Plumlee gives Denver a center with a similar skill-set to pass and cut off of with their bench.
However, for the Nuggets to play that way and incorporate Plumlee as a Jokic-lite option off the bench, the Duke product had to earn his teammates' trust, something that was difficult to do over just 27 games.
But credit Plumlee, who started all 54 games he appeared in with Portland prior to the trade for taking a decreased role off the bench in stride.
"I think his selflessness, his approach to the game, his verticality at the rim are well needed," Connelly said. "And he really added to that locker room."
Denver's defense was a mess this season and it's hard to put stock their defensive numbers, especially from their bench unit with how inconsistent their rotations were throughout the year. The Nuggets allowed 3.6 points per 100 possessions more with Plumlee on the floor than without him, but the four-year veteran had some moments where he looked like a capable backup center on the defensive end of the floor.
Plumlee also logged a healthy amount of minutes alongside Jokic. Denver was a +11 plus-minus in the 175 minutes they played together and the pair actually held opponents to 108.1 points per 100 possessions when they shared the frontcourt. Compare that number to their cumulative Defensive Rating of 110.5 on the year and you can see why Michael Malone likes the idea of Jokic and Plumlee playing together.
Yet, that's a tough sell with Kenneth Faried as your only other option at the backup five and with how well Jokic has played as the lone big in Nuggets' lineups this season.
Plumlee could garner some significant free agent offers this summer as a smart, high IQ big with good instincts, but the Nuggets will certainly have the money to retain Plumlee, if they want, with his status as a restricted free agent.
Expect Denver to act fast on the big man once the free agent moratorium lifts during the first week of July.


text:
Separate names with a comma.
Discussion in 'Lawn Mowing' started by jtrice11, Sep 22, 2005.
Any of you guys have workers that are better than you are at mowing, trimming etc...?
Nope , they learn from me. Im fussy and expect the best. My own yard is my best advertisement . Some people pick up certain jobs and feel comfortable while others I have employed are never going to get it right .
Most of them......... Its their job to be good at it and my job to get them the work to do.......... I love it when I have guys just as good and better than me then I dont have to worry about sending them to any property
Yea, rite. I wish!
No seriously, my guys are pritty good, but not as good or better than I.
Exactly. A smart business mans trys to hire people that are smarter/better at the job than he is.
Boy I wish...thats exactly what im looking for sombody I don't have to babysit.
Well, I have one who picked up the ZTR in about 2 hours and trimming in and hour. He is only 16 and really racks up the hours. He put over 150 hrs (engine time) one of my ZTR this summer. Now, he is better or quicker than I am on it. I have yet to find anyone who can trim a good and quite as me, but I'm still looking....actually I have on who is getting close, but he can't keep up with me all day. Mabe it could be because he makes $12/hr when working and I make $100+ / hr working or $80/hr when stilling back wathing.
Its good to be the owner.
The reason I asked is because my guys can work circles around me. I'm doing everything in my power to keep them busy and get work for them. I still work with them 50% of the time, but I can send them anywhere, give them instructions, and its done to perfection.
Let's hope they don't have better people/business skills than you,because they won't need you for employment if that be the case
Only BG. Chico runs circles around him!!


text:
Leave a Comment:
2 comments
Thank you, thank you, thank you! You just saved my Arc notebook from death! I have been wanting one of these for a long time because I loved the customization abilities. For a while, I used it as a planner, but let's face it… With smartphones, paper planners are obsolete. So it had been sitting around gathering dust. Now, I can wipe that dust off and repurpose this notebook!Reply
[…] Conceptualizing a Novel: Journaling, Plots, and Characterization […]Reply


text:
Thanks Vector
Vector image word art of verbal expressions of gratitude in different languages: English, French, Italian, Spanish and German. Vector type art, beautiful font, similar to hand writing. Vector "Thank you" expressions for all gratitude, foreign languages, speaking, communication and being polite designs.
Thanks Vector
Add Your Review
Anyone know the name of this font? Just wonderful!