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Discussion in 'News and Articles' started by Boanerges(Inactive), Aug 21, 2008.
spider art
Dark Roasted Blend: Spider Webs Glamour & Architecture
Cool webs .
ewwwwwwwwwww yicky nasty i HATE spiders... they are unnatural and creepy... well ok some of them looked nice but the pictures of those really big ones were just grose and i hope i NEVER actually see somthing like that... now im gonna have night mares about being eaten by spiders lol
Hey..Spiders are as natural as mankind! haha..
nice webs!
Indeed! Without spiders pests would over run us quickly- the Lord knew what He was doing.
For the evolutionists- that mindless blob of protoplasm had it all worked out- lol.
Those are incredible pictures.
One thing I found interesting was the point when they noted that a spider uses it's own body to measure the sections of its web.
It reminded me of the units of measurement man used to use, cubits, digits, units, etc.
Maybe if we went back to hand crafting things that way, my hat wouldn't rub on the roof of a car when I drive one! :cap:
They must have used short people cubits!
Bill Cosby:
"Whats a Cubit??"
(One of my all time favorites.)
yes i know lol im glad they are there but i can do with out having to see them lol
-stares..- careful with the short =X
you have one short person here..!
lol lol lol..
take a closer look at them next time..
they're really beautiful creatures!


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Stone, Dirty Sand, Dark Beige Remo Dress
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Price: $538.50
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The dress has no sign of wear in front. It only has two small holes in the tulle section at the bottom of the dress in the back which is not noticeable because that's where there is lot of tulle that gather together.
Style: Remo
Color: Stone
It was purchased from Spain for 1,190.00 Euros.
Size 40 European.
From Pronovias Fiesta Collection of 2014.
I still have the tag with the price on it.
It comes with a tulle shall and a satin belt.
It comes with Pronovias dust bag.
Long beautiful dress. It has stunning embroidery and beads all over the dress, and some more beads and a embroidery flower on one shoulder. The dress is satin underneath and covered all over with a layer of tulle at the top and lots of tulle at the bottom.
Style Tags:
Remo, Remo Stone, Embroidered dress, formal dress, prom, mother of the bride, mother of the groom, long dress, wedding guest, Ball Gown, tulle, beaded, beads,
Mon 11/27/17 - Thu 11/30/17 (4-7 days)
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Another great betting pick yesterday, when AGF beat FC Nordsjælland with 3-1 in Aarhus. It looked well from the beginning but shortly before the break FC Nordsjælland managed to get in the lead after a counter attack, but in the second half AGF showed quality and they could have won even bigger.
Today we look at the Champions League match between FC Barcelona and Celtic. We predict a big win for FC Barcelona and that could seem weird after they just lost at home to Alavez.
Click here to bet --->
But that is exactly the reason why we believe that Barcelona will crush Celtic today. They have something to make up for and a 1-0 victory against a "poor" Celtic team will just not do it. Barcelona will have to show, that the defeat was a one time thing, so they will run over Celtic.
Bet on FC Barcelona with -2.5. Odds 1.77 at bet365.


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We can also arrange a phone or video meeting.
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My next gig required a ride on an airplane.
This artist decided to start a new venture in her career, in Las Vegas, of all places, but that will be the subject of the next blog post. ;-)
The night before that, though, I did a outdoor caricature gig, alongside my dear colleague, Celestia...in the summer....near LAS VEGAS...HOT HOT HOTTTTT....and I survived!!!
Friday, March 3, 2017
Thursday, March 2, 2017
Campground Caricatures!
How about a gig in THIS tranquil locale? Beautiful, isn't it?
However, my gig wasn't on the river bank, but in the campground's air-conditioned clubhouse overlooking the pool:
...and it's one I enjoy doing every year through Entertaining ConXections---and the caricature-loving campground owners (who have their own drawings framed and hanging up in the concessions area:)
Here's a few of the faces from near and far who come to this idyllic peaceful place. :-)
Wednesday, March 1, 2017
Pokemon Birthday Party Caricatures
Check out this backyard: this is a lucky boy with a summer birthday and a mom who's into incredibly fun parties!
She even provided custom paper for me to draw on! What a blast! She also bribed a tween girl to supervise the "pokemon card sign-up sheet" for my caricatures.
Below: the birthday boy with Pikachu!
This was another huuuuge party with dozens of kids and activity, (water slide!!!!!!) so I kept busy drawing and not photographing. Couldn't resist getting a shot of this little one, though!
Thanks to the-Nose.com!
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Carrie Underwood's toughest audience is living in her belly. The pregnant singer, 31, told Jimmy Fallon on Monday, Dec. 8, that she performs for her yet-to-be-born son, and feels pressure to make it "good."
"I feel like he kind of feels… he hears me singing whether he would like that or not," Underwood said on The Tonight Show. "I mean, that's what I do, he doesn't have a choice."
"I do feel like I have to sing good all the time," the "Something in the Water" performer continued. "Like, if I'm just in my car driving, I feel like I have to make it good because he's listening. He's always in there."
Underwood is expecting her first child with husband Mike Fisher. The pair, who wed in July 2010 after getting engaged in December 2009, announced their happy news this past September. The country crooner isn't the only one taking the stage in the couple's Nashville home, though; hockey player Fisher, 34, also exercises his pipes around his talented wife.
"It's always surprising," the American Idol alum told Fallon of Fisher. "He doesn't actually know the real lyrics to any song, except for like, 'Whoomp! (There It Is)' will come on the radio and he knows every word to that. Yet, I'll ask him what my current single is and he sings all the wrong words. He has no clue."
Due to welcome a son next year, Underwood also dished to Fallon about how her family members took the news of her pregnancy. See photos of Carrie's adorable baby bump.
"I have lots of nieces and nephews so my parents were obviously very happy, but they've heard it all before," the Oklahoma-born star explained. "They have great grandchildren. I'm slow."
"Mike's mom shed a few tears…" Underwood added of the Canadian hunk's family. "She was that kind of happy."
Watch Underwood talk pregnancy and performances in the video above!
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Vrije Universiteit Amsterdam
VU University Amsterdam
Parallel and Distributed Computer Systems
Program Information
Degrees Offered:
Parallel and Distributed Computer Systems
Format: Campus
Program Description:
Parallel and Distributed Computer Systems emphasizes experimental computer science rather than theory, and typically involves the design, implementation, testing and performance analysis of complex systems software. The curriculum includes many software lab courses, which are aimed at training you to carry out scientific software experiments that solve challenging research problems. For some projects, you have to cooperate in small teams to train you in collaborative research.


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Financial Intelligence is part of the Business Intelligence Division of Informa PLC
This is operated by a business or businesses owned by Informa PLC and all copyright resides with them. Informa PLC's registered office is 5 Howick Place, London SW1P 1WG. Registered in England and Wales. Number 8860726.
For a pdf version of this report, click here.
Snapshot - Valuation of 1Q2017 APAC USD new issues
A snapshot of valuations as of March 31 for all USD deals/tranches issued by APAC issuers in 1Q2017 reflect a strong preference for higher yielding issues. Chinese HY, Indonesian, Indian and Financials HY Subordinated USD bonds issued in 1Q2017 were arguably amongst the better performers for the quarter. Of course, there will be certain bonds within the other groups that would also have performed well but those were the four distinct groups that we thought saw strong performance collectively at the end of 1Q2017 based on the data studied below.
Quick notes on the data:
It is unsurprising to see new issues from Indonesia and India performing well by the end of 1Q2017. Both economies have performed well YTD underpinned by its strong domestic consumption aiding growth while the recovery in the global economy stoked an export rebound. The Jakarta Composite Index and the SENSEX index had set all-time highs recently at 5,680.239 on April 6 and 29,620.50 on March 31 respectively.
Chinese USD bonds have seen good demand and strong performance particularly for the new Corporate high yield issues although as with all things, caveat emptor should not be taken lightly
Looking Ahead:
1Q2017 saw total notable USD issuance of USD122.18bn. In terms of APAC USD bond issuances for 2Q2017, June is typically a slow month while French elections coming up in late April/early May could cause some volatility which may lead to issuers preferring to wait on the sidelines. However with China having a total USD1.4tr debt maturing in 2017, most of which will require refinancing over the coming months, we may see more issuances coming from the mainland. Certain issuers (i.e. the better performing sub-groups in this study such as Indian, Indonesian corporates for example) may take advantage of the good demand and performance of their peer group and opportunistically tap the USD market in the next quarter.
If you have questions about how Informa Financial Intelligence can assist your business, please fill out the form below and we'll get back to you shortly.
If you prefer to get in touch by phone, please refer to the About section of our website for a list of our offices and contact details.
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Danil was a male Benthan active as an officer in the Benthan Guard in the early 25th century.
In 2410 Danil held the rank of captain. Following the arrival of Alpha Quadrant forces in the Delta Quadrant, Danil attempted to arrest Ferengi DaiMon Qwen in the Kendren system for smuggling, but his Benthan assault cruiser was disabled by Hazari and Hierarchy vessels also after Qwen. An Alpha Quadrant Alliance starship drove off the attackers and repaired his ship and Qwen's. (STO - "With Friends Like These..." mission: "A Deal is a Deal")
External link Edit
- Danil article at The Star Trek Online Wiki.


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Happy Thanksgiving! This week, the Thanksgiving holiday propels us into another holiday season. All around us, people seem to be planning and celebrating. Friends and family express thanks for their many blessings; Christmas music plays on the radio; holiday cards arrive in the mail; decorations are hung; and plans are made to reconnect with friends and family. But, amidst the joy, laughter and sparkle, for those grieving the loss of a loved one, this time of year is fraught with anxiety, stress and maybe even dread.
Over the years, Hospice of the Red River Valley has offered various articles and tips for coping with grief. And, this year, we are pleased to share grief information with you in our upcoming Confidence in Care webinar series. Please join us for this informational webinar series where you'll learn about:
Anticipatory Grief
Tuesday, November 27, 2-3 p.m.
This webinar will define anticipatory grief and dispel myths associated with this type of grief. Participants will explore different types of anticipatory loss and identify challenges and issues faced by individuals and families dealing with anticipatory grief. Click here to register.
Grief and Loss Awareness
Tuesday, December 4, 2-3 p.m.
Participants in this webinar will learn about various types of grief, factors that influence grief and suggestions for successfully handling grief. They will also learn how to identify warning signs of complicated grief, and how to seek help for those dealing with complicated grief issues. Click here to register.
Professional Caregiver Grief and Grief in the Workplace
Tuesday, December 11, 2-3 p.m.
This webinar will provide a way for health care professionals to explore issues of grief and loss as it relates to their roles as professional caregivers. Participants will also learn strategies to help grieving co-workers. Click here to register.
Please note: While these webinars are intended for health care professionals (nurses, certified nursing assistants, social workers, chaplains, physicians, etc.), the series is free and open to anyone who might find value in this information.
This Thanksgiving, we are so thankful for you—our readers, supporters, advocates and partners in care. We are truly blessed to live and work in the communities we call home. Happy Thanksgiving!
For more information about Hospice of the Red River Valley, please visit our website or contact us at 1-800-237-4629.


text: I Wish My Life Was Better Me Too Personal Stories, Advice, and Support 29 People Report Group Sort popular recent I wish my life were better. I wish that I was smarter in my youth.I wish that I had made different choices. carrie71 46-50, F 1 Response 1 Feb 27, 2016 More Stories


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Fr. Ron Rolheiser, omi
I work and move within Church circles and find that most of the people I meet there are honest, committed and for the most part radiate their faith positively. Most churchgoers aren't hypocrites. What I do find disturbing though is that too many of us can be bitter, angry, mean-spirited and judgmental, especially in terms of the values that we hold most dear. more . . .
Read the rest of entry »
If you can keep your head when all about you
Are losing theirs and blaming it on you.
You will recognize these words as the opening lines of Rudyard Kipling's famous poem, If, and they, as much as any scriptural commentary, provide the key to understand the story of Noah and the Ark. more . . .
A comedian recently quipped that today's information technologies have effectively rendered a number of things obsolete, most notably phonebooks and human courtesy. That's also true for human rest.more . . .
Eighty-five years ago, G. K. Chesterton looked at his society and saw some things that disturbed him. Here's his comment:
"There comes an hour in the afternoon when the child is tired of 'pretending'; when he is weary of being a robber or a noble savage. It is then that he torments the cat. There comes a time in the routine of an ordered civilization when the man is tired at playing at mythology and pretending that a tree is a maiden or that the moon made love to a man. more . . .
The great Jewish prophets, the forerunners of Jesus, coined a mantra which ran something like this: The quality of your faith will be judged by the quality of justice in the land and the quality of justice in the land will be judged by how "widows, orphans and strangers" (biblical code for the three most vulnerable groups in society) fared while you were alive. more . . .
They hadn't understood about the loaves! The Gospels use those words to describe the crowd that Jesus had miraculously fed with five barley loaves and two fish. They ate, but they didn't understand. What didn't they understand? more . . .
The past several weeks have been some of most pressured weeks in my life. I have been trying to balance the pressures of teaching a three-hour-a-day intersession course, my duties as an administrator, a series of emergencies to do with the deaths of a couple of close friends, along with trying to sustain some kind of prayer life, all the time nursing a nasty viral cold. It's been a pressured time. more . . .
Margaret Laurence's novel, A Jest of God, tells the story of two sisters: One of them, Rachel, single still and childless at mid-life, is a gifted, elementary school teacher. The other is a stay-at-home-mother, dedicating herself to full-time caring for her children. more . . .
Mary gave birth to a son, her firstborn. She wrapped him in swaddling clothes and laid him in a manger because there was no room for them in the inn. more . . .
Like a deer yearns for flowing streams, so my soul yearns for you my God." "My soul keeps vigil for you in the night." more . . .


text: Hello, new to the forum. In the market of a new mower....looking at the kubota zd28, but I hear it's on the heavy side, so I need some advice on what would be my best option. Most of the lawns I have are around a lake...wet, soft, and muddy..(don't want to get stuck)..lots of hills too....trees and shrubs. The most popular grass that I'm cutting is Centipede with the occasional Bermuda. Please any suggestions or advice you might have for me I would really appreciate it...my biggest concern is getting stuck in the mud, and tackling them hills.


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|Publication number
|Publication type
|Application number
|Publication date
|Sep 24, 1996
|Filing date
|Jun 7, 1995
|Priority date
|Nov 8, 1989
|Fee status
|Also published as
|Publication number
|Mary K. Estes, Xi Jiang, David Y. Graham
|Original Assignee
|Baylor College Of Medicine
|Export Citation
|BiBTeX, EndNote, RefMan
|Non-Patent Citations (51), Referenced by (41), Classifications (13), Legal Events (5)
|External Links: USPTO, USPTO Assignment, Espacenet
This is a continuation of application Ser. No. 07/433,492, filed Nov. 8, 1989 now abandoned.
The present invention relates generally to synthesizing clones of Norwalk virus and to making probes to Norwalk and related viruses. It also relates to methods of detection and characterization of Norwalk and related viruses.
Norwalk virus is one of the most important viral pathogens causing acute gastroenteritis, the second most common illness in the United States (Dingle et al., 1953; Kapikian and Chanock, 1985). Up to 65% of cases of viral gastroenteritis have been estimated to be caused by Norwalk or Norwalk-like viruses (Kaplan et al., 1982). Both water and foodborne transmission of Norwalk virus has been documented, and particularly large epidemic outbreaks of illness have occurred following consumption of contaminated shellfish including clams, cockles, and oysters (Murphy et al., 1979; Gunn et al., 1982; Wilson et al., 1982; Gill et al., 1983; DuPont 1986; Morse et al., 1986; Sekine et al., 1989). An increase in fish and shellfish-related food poisonings has recently been noted and attributed to increased recognition of these entities by clinicians as well as to increased consumption of seafood (Eastaugh and Shepherd, 1989). Norwalk virus was discovered in 1973, but knowledge about the virus has remained limited because it has failed to grow in cell culture and no suitable animal models have been found for virus cultivation. Therefore, human stool samples obtained from outbreaks and from human volunteer studies are the only source of virus. Moreover, the concentration of the virus in stool is usually so low that virus detection with routine electron microscopy is not possible (Dolin et al., 1972; Kapikian et al., 1972; Thornhill et al., 1975). Current methods of Norwalk virus detection include immune electron microscopy and other immunologic methods such as RIAs or a biotin-avidin ELISAs which utilize acute and convalescent phase serum from humans. To date, no hyperimmune serum from animals has been successfully prepared due either to insufficient quantities or unusual properties of the viral antigen. Preliminary biophysical characterization of virions has indicated particles contain 1 polypeptide (Greenberg et al., 1981), but efforts to characterize the viral genome have failed. Therefore, these viruses have remained unclassified.
1. Dingle J., Badger G., Feller A. et al. 1953. A study of illness in a group of Cleveland families: 1. Plan of study and certain general observations. Am. J. Hyg. 58:16-30.
2. Dolin R., Blacklow N. R., DuPont H., Buscho R. F., Wyatt R. G., Kasel J. A., Hornick R., and Chanock R. M. 1972. Biological properties of Norwalk agent of acute infectious nonbacterial gastroenteritis. Proc. Soc. Exp. Med. and Biol. 140:578-583.
3. Dolin R., Blacklow N. R., DuPont H., Formal S., Buscho R. F., Kasel J. A., Chames R. P., Hornick R., and Chanock R. M. 1971. Transmission of acute infectious nonbacterial gastroenteritis to volunteers by oral administration of stool filtrates. J. Infect. Dis. 123:307-312.
4. DuPont H L. 1986. Consumption of raw shellfish--is the risk now unacceptable? New Engl. J. Med. 314:707-708.
5. Eastaugh J, Shepherd S. 1989. Infectious and toxic syndromes from fish and shellfish consumption. Arch. Intern. Med. 149:1735-1740.
6. Gill O. N., Cubitt W. D., McSwiggan D. A., Watney B. M. and Bartlett C. L. R. 1983. Epidemic of gastroenteritis caused by oysters contaminated with small round structured viruses. Br. Med. J. 287:1532-1534.
7. Greenberg H. B., Valdesuso J. R., Kalica A. R., Wyatt R. G., McAuliffe V. J., Kapikian A. Z. and Chanock R. M. 1981. Proteins of Norwalk virus. J. Virol. 37: 994-999.
8. Gunn R. A., Janowski H. T., Lieb S., Prather E. C., and Greenberg H. B. 1982. Norwalk virus gastroenteritis following raw oyster consumption. Am. J. Epidemiol. 115:348-351.
9. Jiang X., Estes M. K., and Metcalf T. G. 1989. In situ hybridization for quantitative assay of infectious hepatitis A virus. J. Clin. Microbiol. 27:874-879.
10. Jiang X., Estes M. K., and Metcalf T. G. 1987. Detection of hepatitis A virus by hybridization with single-stranded RNA probes. Appl. Environ. Microbiol. 53:2487-2495.
11. Jiang X., Estes M. K., Metcalf T. G., and Melnick J. L. 1986. Detection of hepatitis A virus in seeded estuarine samples by hybridization with cDNA probes. Appl. Environ. Microbiol. 52:711-717.
12. Kapikian A. Z. and Chanock R. M. 1985. Norwalk group of viruses. In: BN Fields (ed.) Virology, Raven Press, New York, pp. 1495-1517.
13. Kapikian A. Z., Wyatt R. G., Dolin R., Thornhill T. S., Kalica A. R., and Chanock R. M. 1972. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J. Virol. 10:1075-1081.
14. Kaplan J., Feldman R., Campbell D. et al. 1982. The frequency of a Norwalk-like pattern of illness in outbreaks of acute gastroenteritis. Am. J. Public. Health 72:1329-1332.
15. Morse D. L., Guzewich J. J., Hanrahan J. P., Stricof R., Shayegani M., Deibel R., Grabau J. C., Nowak N. A., Herrmann J. E., Cukor G., and Blacklow N. R. 1986. Widespread outbreaks of clam- and oyster-associated gastroenteritis: role of Norwalk virus. New Engl. J. Med. 314:678-681.
16. Murphy A. M., Grohmann G. S., Christopher P. J., Lopez W. A., Davey G. R, and Millsom R. H. 1979. An Australia-wide outbreak of gastroenteritis from oysters caused by Norwalk virus. Med. J. Aust. 2:329-333.
17. Sekine S., Okada S., Hayashi Y., Ando T., Terayama T, Yabuuchi K., Miki T., and Ohashi M. 1989. Prevalence of small round structured virus infections in acute gastroenteritis outbreaks in Tokyo. Microbiol. Immunol. 33:207-217.
18. Thornhill T. S., Kalica A. R., Wyatt R. G., Kapikian A. Z., and Chanock R. M. 1975. Pattern of shedding of the Norwalk particle in stools during experimentally induced gastroenteritis in volunteers as determined by immune electron microscopy. J. Infect. Dis. 132:28-34.
19. Wilson R., Anderson L. J., Holman R. C., Gary G. W., and Greenberg H. B. 1982. Waterborne gastroenteritis due to the Norwalk agent: clinical and epidemiologic investigation. Am. J. Public Health 72:72-74.
FIG. 1. EM picture of Norwalk viruses after CsCl gradient purification.
FIG. 2a Hybridization of stool samples with 32 P labeled plasmid DNA for screening positive Norwalk cDNA clones. Nucleic acids from paired stools [before (b) and after (a) infection with Norwalk virus] from two volunteers (1 and 2) were dotted on Zetabind filters. Replicate strips were prepared and hybridized at 50� C. and 65� C. with each test clone (pUC-27, pUC-593, pUC-13 and pUC-953). One clone (pUCNV-953) which reacted only with stool samples after (but not before) Norwalk infection was considered as a potential positive clone and was chosen for further characterization.
FIG. 2b. Dot blot hybridization of clone pUCNV-953 with another 3 sets of stool samples collected at different times after infection (B=before acute phase of illness; A=acute phase of illness; P=post-acute phase of illness) of 3 volunteers. The nucleic acids were dotted directly or after treatment with RNAse or with DNAse before dotting. Double stranded homologous cDNA (pUCNV-953) was dotted after the same treatments as the stool samples.
FIG. 3. Dot blot hybridization of Norwalk viruses in a CsCl gradient with ssRNA probes made from pGEMNV-953. Aliquots of 50 ul from each fraction in a CsCl gradient were dotted onto a Zetabind filter. Duplicates of filters were made and hybridized with the two ssRNA probes respectively. The two strands were subsequently called cRNA (positive hybridization with the vital nucleic acid) and vRNA (no hybridization with the viral nucleic acid). Graph shows EM counts of Norwalk viruses from each fraction of the same CsCl gradient for the dot blot hybridization. Five squares from each grid were counted and the average of the number of vital particles per square was calculated.
FIG. 4. Hybridization of Norwalk viral RNA with clone pUCNV-953. Nucleic acids extracted from partially purified viruses were electrophoresed in a native agarose gel as described previously (Jiang et al., 1989). The gel was then dried at 80� C. for 1 h and hybridized with 32 P labeled pUCNV-953 insert. Lane 1, 23 S and 16 S rRNA from E. coli (Miles Laboratories Inc., Naperville, Ill. 60566), lanes 2 and 4, total nucleic acids from partially purified stool samples containing Norwalk virus, and lane 3, HAV RNA.
FIG. 5. The nucleotide sequence of the genome sense strand of the Norwalk virus cDNA clone. The deduced amino acid sequence of a long open reading frame in this cDNA is also shown.
Norwalk virus was produced by administration of safety tested Norwalk virus (8FIIa) to adult volunteers. The virus inoculum used in the volunteer study, was kindly supplied by Dr. Albert Kapikian (Laboratory of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md.). This virus originated from an outbreak of acute gastroenteritis in Norwalk, Ohio (Dolin et al., 1971). Two ml of a 1 to 100 dilution of 8FIIa in TBS was administered orally to each individual with 80 ml of milli-Q water (Millipore, Bedford, Mass. 01730). Sodium bicarbonate solution was taken by each person 2 min before and 5 min after virus administration. The volunteer studies were approved by the Institutional Review Board for Human Research at Baylor College of Medicine, at the Methodist Hospital and at the General Clinical Research Center. The virus was administered to the volunteers in the General Clinical Research Center where the volunteers were hospitalized and under extensive medical care for 4 days. All stools were collected and kept at -70� C. for later use.
A 10% solution of stool samples in TBS was first clarified by low speed centrifugation at 3000 rpm for 15 min. The resultant supernate was then extracted 2 to 3 times with genetron in the presence of 0.5% Zwittergent 3-14 detergent (Calbiochem Corp., La Jolla, Calif.). Viruses in the aqueous phase were concentrated by pelleting at 36,00 rpm for 90 min through a 40% sucrose cushion in a 50.2 Ti rotor (Beckman Instruments, Inc., Palo Alto, Calif. 94304). The pellets were suspended in TBS and mixed with CsCl solution (refractive index 1.368) and centrifuged at 35,000 rpm for 24 h in a SW50.1 rotor (Beckman). The CsCl gradient was fractioned by bottom puncture and each fraction was monitored for virus by EM examination. The peak fractions containing Norwalk virus were pooled and CsCl in the samples was diluted with TBS and removed by pelleting the viruses at 35,000 rpm for 1 h. The purified virus was stored at -70� C.
Purified Norwalk virus from CsCl gradients was first treated with proteinase K (400 ug/ml) in proteinase K buffer (0.1M Tris-Cl pH 7.5, 12.5 mM EDTA, 0.15M NaCl, 1% w/v SDS) at 37� C. for 30 min. The samples were then extracted once with phenol-chloroform and once with chloroform. Nucleic acids in the aqueous phase were concentrated by precipitation with 2.5 volumes of ethanol in the presence of 0.2M NaOAc followed by pelleting for 15 min in a microcentrifuge.
Nucleic acids extracted from the purified Norwalk viruses were denatured with 10 mM CH3 HgOH and cDNA was synthesized using the cDNA synthesis kit with the supplied random hexanucleotide primer (Amersham, Arlington Heights, Ill. 60005). After the second strand synthesis, the reaction mixture was extracted once with phenol-chloroform and once with chloroform followed by ethanol precipitation. Amplification of DNA was performed using the random prime kit for DNA labeling (Promega Corp., Madison, Wis. 53711-5305). Eight cycles of denaturation (100� C. for 2 min), reanealing (2 min cooling to room temperature) and elongation (room temperature for 30 min) were performed after addition of Klenow fragment (Promega Corp.). A DNA library was constructed in pUC-13 with blunt-end ligation into the Sma I site.
White colonies from transformed DH5 alpha bacterial cells (BRL) were picked and both a master plate and minipreps of plasmid DNA was prepared for each clone. Clones containing inserts were identified after electrophoresis of the plasmid DNA in an agarose gel. The DNA in the agarose gel was cut out and labeled with 32 P using the prime-a-gene labeling system (Promega Corp.). Nucleic acids extracted from paired stool samples (before and after Norwalk infection) from two volunteers (543 and 544) were dotted onto Zetabind filters (AFM, Cuno, Meriden, Conn.). Replicate filter strips were prepared and hybridized with each labeled plasmid probe individually at 68� C. without formamide. Potential positive clones were judged by their different reactions with the pre- and post-infection stools. Clones which reacted with post-(but not pre-) infection stools of volunteers were considered positive and these clones on the master plates were characterized further.
To permit better diagnosis and molecular characterization of Norwalk virus, we have made a cDNA library derived from nucleic acid extracted from virions purified from stool samples. Norwalk virus was purified with methods used previously for hepatitis A and rotaviruses from stool samples with some modifications (Jiang et al., 1986). Basically stool samples obtained from volunteers administered Norwalk virus were treated with Genetron to remove lipid and water insoluble materials. Virus in the aqueous phase was then pelleted through a 40% sucrose cushion. The resultant pellets were resuspended, sonicated and loaded in a CsCl gradient for isopycnic centrifugation. FIG. 1 shows an electron micrograph of purified Norwalk viruses after CsCl gradient centrifugation. Approximately 109 physical particles were obtained from 500 grams of stools.
A cDNA library was generated from nucleic acids extracted from these purified viruses by proteinase K treatment of the samples followed by phenol-chloroform extraction and ethanol precipitation (Jiang et al., 1986; 1987). Because the nature of the viral genome was unknown, the extracted nucleic acids were denatured with methylmercuric hydroxide before cDNA synthesis. Random primed cDNA was synthesized with the Gubler-Hoffman method (cDNA synthesis system plus, Amersham) and a small amount of cDNA was obtained. Direct cloning of this small amount of cDNA was unsuccessful. Therefore, a step of amplification of the DNA was performed by synthesizing more copies of the DNA with random primers and the Klenow fragment of DNA polymerase before cloning. The procedure involved cycles of denaturation, addition of random primers and the Klenow fragment of DNA polymerase, reannealing and elongation. With this procedure, a linear incorporation of labeled nucleotides into product was observed as the number of cycles of synthesis was increased. The numbers of cycles performed were limited (<10) to avoid the synthesis of an excess of smaller fragments. In the case of Norwalk cDNA, 8 cycles of amplification were performed and approximately 2.5 ug of DNA were obtained, which was at least a 100-fold amplification of the starting template cDNA. This amplified cDNA was cloned into pUC-13 by blunt end ligation and a positive clone (pUCNV-953) was isolated.
To obtain the positive Norwalk virus clone, minipreparations of the plasmid DNAs containing potential inserts were screened by agarose gel electrophoresis. The larger clones in the gel were cut out and probes were made with the DNA in the gel using the prime-a-gene labeling system (Promega Corp.). These probes were hybridized individually with paired stool samples (before and after Norwalk infection) from two volunteers (FIG. 2a). One clone (pUCNV-953) reacted with post- but not pre-infection stool samples from both volunteers.
To further confirm the vital origin of the clone pUCNV-953, 6 more paired stool samples were tested and the same results were obtained. FIG. 2b shows a dot blot hybridization of the clone with stool samples collected at different times post-infection of the disease. Strong signals were observed only with stools from acute phase, but not before and after the illness. This result was consistent with previous RIA assays for viral antigen detection using convalescent sera from volunteers with Norwalk diarrhea and immune electron microscopy (IEM) studies of the samples for viral particle examination. This result also agrees with the patterns of virus shedding in stool in the course of the disease (Thornhill et al., 1975 ). When the clone was hybridized with fractions of a CsCl gradient from the Norwalk virus purification scheme, a correlation between hybridization and EM vital particle counts was observed (FIG. 3). The peaks of the hybridization signals and viral particle counts both were at fractions with a density of 1.38 g/cm3 which agrees with 9revious reports of the biophysical properties of Norwalk virus. Finally, the clone was tested by hybridization with highly purified Norwalk virus electrophoresed on an agarose gel. A single hybridization band was observed with Norwalk virus but not with HAV and rotavirus (FIG. 4). Sequence analysis of the pUCNV-953 cDNA showed this clone is 511 bp (FIG. 5); this partial genomic cDNA encodes a potential open reading frame (FIG. 5). No significant nucleotide or deduced amino acid sequence homology was found by comparison with other sequences in the Gen Bank (Molecular Biology Information Resource, Eugene Software, Baylot College of Medicine).
We also used this first Norwalk virus cDNA to begin to characterize the viral genome. The pUCNV-953 cDNA was subcloned into the transcription vector pGEM-3Zf(+) and ssRNA probes were generated by in vitro transcription using SP6 and T7 polymerases (Promega). When two opposite sense ssRNA probes were hybridized with the vital nucleic acid separately, only one strand reacted with the virus, indicating the viral genome is single stranded. As shown in FIG. 2b, the hybridization signals were removed by treatment of the viral nucleic acid with RNAse (but not with DNAse) before loading them onto the filters, indicating the virus genome contains ssRNA. A long open reading frame was found in one of the two strands of the inserted DNA by the computer analysis of the sequences of pUCNV-953. The ssRNA probe with the same sequence as this coding strand did not react with the vital nucleic acid, but the complementary ssRNA probe did react in the hybridization tests. Therefore, Norwalk virus contains a positive stranded RNA genome. The size of the genome of Norwalk virus was estimated to be about 8 kb based on comparisons of the migration rate of the purified vital RNA in agarose gels with molecular weight markers. This size is slightly bigger than that of the picornaviruses [HAV and poliovirus; (FIG. 4)].
This cDNA or fragments thereof can be used in assays to detect the genome of Norwalk and other related viruses. The detection assays can include labeled cDNA or ssRNA probes for direct detection of the Norwalk virus genome. Alternatively, small oligonucleotide probes and polymerase chain reaction amplification can also be used to detect the Norwalk and related virus genomes. Expression of the open reading frame in the cDNA can be used to make hyperimmune or monoclonal antibodies for use in diagnostic products and vaccines.
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text: Can you please help me? I love 3d animations and im totally new to 3d animations.I m searching for some EASY 3D animation program that i can learn easy.i know all animation program Blender..Maya..Carrara...3DS Max..Can you reccomend me EASIEST that you know...easy for learning?i want to make short 3d cartoon. Thanks for Help.


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raj varma
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indian greenhorn
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Hello, it is not possible to put the image directly on the JFrame or on a JPanel. One way to make it is to set the image (should have good size)on a JLabel and when you add the JLabel it will appear as it is a background image. You can also add components over this image. This way you can achieve your requirement of background image to the JFrame.
Nathan Pruett
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You can't draw it to the JFrame itself (just like the previous poster said), but instead of using JLabel, I would create a custom panel to display the image so I could add components on top of it easily. Then you can set that panel as the content pane of the frame. Below is some code I had that shows how to do this:
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|Episode 6456
|Production code
|ITV transmission date
| 27th December 2006
|Script editor
| Sarah Mann
Angela Sinden
|Story associates
| Martin Sterling
Hamish Wright
Kathryn Pugsley
John Kerr
Kate Rogerson
|Damon Rochefort
|Alan Davis
|Tim O'Mara
|Executive Producer
|Kieran Roberts
|Steve Frost
|Previous episode
|25th December 2006
|Next episode
|29th December 2006
Jason tries to talk Sarah round but she's upset that he's let her down again. Shirley Wheeton from Social Services call at No.11. She promises to try and track down the baby's mother but in the meantime it's decided it would be best for her to stay with Eileen. Audrey tries to apologise to Maureen for her affair with Bill but a row ensues and Sally has to take control calling Bill, Audrey and Maureen a bunch of randy geriatrics. Molly continues to train Jack like she would a dog. Vera's amazed at the results. Bill thanks Audrey for the fun they had together but explains that he has to try and win Maureen back. Audrey's disappointed. Les and Yana proudly present Cilla with £600 which they raised. The regulars clap and Cilla's touched. She swans out saying she's going to book her trip to Florida. Tracy suggests to Charlie that in order for her to get her own back she should sleep with Liam. Charlie thinks she's joking. Eileen forces Jason to accept some responsibility and leaves him looking after his baby although Jason still tries to deny it's his.
Regular castEdit
- Eileen Grimshaw - Sue Cleaver
- Violet Wilson - Jenny Platt
- Sean Tully - Antony Cotton
- Jason Grimshaw - Ryan Thomas
- Cilla Battersby-Brown - Wendi Peters
- Gail Platt - Helen Worth
- Sarah Platt - Tina O'Brien
- Les Battersby-Brown - Bruce Jones
- Jack Duckworth - William Tarmey
- Vera Duckworth - Elizabeth Dawn
- Tyrone Dobbs - Alan Halsall
- Molly Compton - Vicky Binns
- Sally Webster - Sally Whittaker
- Kevin Webster - Michael Le Vell
- Bill Webster - Peter Armitage
- Audrey Roberts - Sue Nicholls
- Maureen Webster - Sherrie Hewson
- Blanche Hunt - Maggie Jones
- Deirdre Barlow - Anne Kirkbride
- Liz McDonald - Beverley Callard
- Charlie Stubbs - Bill Ward
- Tracy Barlow - Kate Ford
- Liam Connor - Rob James-Collier
- Ken Barlow - William Roache
Guest castEdit
- Coronation Street exterior
- Rovers Return Inn - Public
- 1 Coronation Street - Back room and kitchen
- 8 Coronation Street - Downstairs rooms
- 9 Coronation Street - Back room/kitchen
- 11 Coronation Street - Back room and kitchen
- 13 Coronation Street - Front room
- Final appearance of Sherrie Hewson as Maureen Webster.
- This episode was transmitted at 8.30pm.
- TV Times synopsis: Tracy steps up her revenge campaign against two-timing boyfriend Charlie by suggesting she have an affair of her own; and Jason is outraged when Eileen decides to keep the baby.
- Viewing Figures: UK broadcast - 9,870,000 viewers (6th place).
|December 2006 episodes
| Fri 1st (1 2) • Sun 3rd • Mon 4th (1 2) • Wed 6th • Fri 8th • Sun 10th • Mon 11th (1 2) • Wed 13th • Fri 15th
Sun 17th • Mon 18th (1 2) • Wed 20th • Fri 22nd • Sun 24th • Mon 25th • Wed 27th • Fri 29th • Sun 31st


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ANOTHER toxic canister has been found on a beach in Queensland.
Canisters of aluminium phosphide - rat poison, which can be fatal if inhaled or ingested - have been washing up on Queensland and NSW beaches over the past 12 months.
The canister was found on Flinders Beach on North Stradbroke Island on Tuesday afternoon.
Police are urging people not to open the canisters or move them but ring triple zero.
Originally published as Another toxic canister found on Qld beach


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The European Union's Enlargement Process and the Collective Identity Formation in Turkey; The interplay of multiple identities
This paper analyzes the impact of the EU enlargement process on Turkish collective identity formation. EU conditionality influences Turkish collective identity by bringing to the forefront issues on democracy, civilian control of the military, impartiality and independence of the judiciary, and minority rights. The paper investigates how different social and political groups in Turkey react to the reform process and argues that there is an ongoing adaptation process in Turkish politics to the EU political criteria and norms parallel to the accession negotiations with the EU. The latter is important at two different levels: First, Turkey increasingly accepts the EU norms in civil-military relations, human rights and recognition of minority rights. Second, these changes and diffusion of norms from the EU into Turkey generate a transformation of the Turkish collective identity.
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text:
I bet she is a laugh a minute
Here's a link to this photo. Just copy and paste!
Here is direct link of this photo
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[URL=https://www.arrse.co.uk/community/gallery/photos/if-you-were-the-only-girl-in-the-world.6837/]If you were the only girl in the world[/URL] by [URL=https://www.arrse.co.uk/community/gallery/authors/naguere.10684/]naguere[/URL] on Army Rumour Service
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Kimberly LeChasseur
Associate Research Professor
Associate Research Professor
Associate Editor, Educational Administration Quarterly
Academic Degrees:
Ph.D. Urban Education, Temple University, 2009.
M.Ed. Urban Education, Temple University, 2008.
A.B., Liberal Arts, College of the Holy Cross, 2004.
Areas of Expertise:
Urban Education and Reform
Social Justice and Educational Equity
Community Partnerships
Data-based Decision Making
Kimberly LeChasseur is an Assistant Research Professor with the Center for Education Policy Analysis at the University of Connecticut. Her current research centers around issues of equity and access in education, with particular attention to school contexts as shaped by instructional quality and leadership practices. She is also deeply interested in data-driven decision-making, both in schools and in other learning communities, such as collaborations across community stakeholders. At UConn, Kimberly is involved in documenting early literacy partnerships across the state, multiple studies of teacher quality across contexts, and examining adult literacy in the Greater Hartford Region.
Selected Publications & Reports:
LeChasseur, K., Welton, A. J., Mayer, A., & Donaldson, M. (in press). Situating teacher inquiry: A micropolitical perspective. School Effectiveness and School Improvement.
Donaldson, M., Woulfin, S., LeChasseur, K., & Cobb, C. (in press). The structure and substance of teachers' opportunities to learn about teacher evaluation reform: Promise or pitfall for equity? Equity & Excellence in Education.
LeChasseur, K. (2014). Critical race theory and the meaning of "community" in district partnerships. Equity and Excellence in Education, 47(3), 305-320.
LeChasseur, K. (2014). Collaboration, performance management, and sustainability: Case studies from Discovery Collaboratives. Report to the William Caspar Graustein Memorial Fund. Storrs, CT: Center for Education Policy Analysis, University of Connecticut.
Donaldson, M., Cobb, C. D., LeChasseur, K., Gabriel, R., Gonzales, R., Woulfin, S., & Makuch, A. (2014). An evaluation of the pilot implementation of Connecticut's System for Educator Evaluation and Development. Report to the Connecticut General Assembly. Storrs, CT: Center for Education Policy Analysis, University of Connecticut.
LeChasseur, K. (2014). The role of legitimacy in collaboration. Discovery Levers of Change Brief. Report to the William Caspar Graustein Memorial Fund. Storrs, CT: Center for Education Policy Analysis, University of Connecticut.
Donaldson, M. L., Mayer, A., LeChasseur, K., Welton, A., & Cobb, C. (2013). New roles for teachers unions?: Reform unionism in school decentralization. Journal of Educational Change, 14(4), 501-525.
Mayer, A., LeChasseur, K., Donaldson, M., & Cobb, C. (2013). Organizational learning as a model for continuous transformation. Journal of Planning and Changing, 44(4).
Mayer, A. P., & LeChasseur, K. (2013). Caught in the middle: Principals' attempts to achieve school autonomy and devolve decision-making. Journal of Urban Learning, Teaching and Research, 9, 33-42.
LeChasseur, K., Mayer, A., & Woulfin, S. (2013). Collaboration and performance management: The Early Literacy Partnership in six Connecticut communities. Report to the Connecticut Center for School Change. Storrs, CT: Center for Education Policy Analysis, University of Connecticut.
Mayer, A., LeChasseur, K., Donaldson, M. L., Warhol, L., Cobb, C., & Welton, A. (2013). Leading autonomous schools: Negotiating new district and school leadership roles. Educational Administration Quarterly, 49(5), 695-731.
Outstanding Policy Report Award, 2015, Division L of the American Educational Research Association for "An Evaluation of the Pilot Implementation of Connecticut's System for Educator Evaluation and Development"
William J. Davis Award, 2014, University Council for Education Administration
Measures of Effective Teaching Early Career Award, 2013-2014, National Academy of Education
Samuel S. Fels Fund Summer Fellowship, 2006, Philadelphia Youth Network
|860 486 2403
|Mailing Address
|Unit 3093
|Office Location
|Gentry 202


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Lamb and Orzo Stuffed Pepper with Chunky Tomato Sauce
Photo by Romulo Yanes
- 1 small orange bell pepper (6 to 8 oz)
- 1 medium onion
- 1 ½ tablespoons fresh dill
- ¼ teaspoon salt
- ¼ pound ground lamb
- 3 tablespoons orzo
- 2 medium tomatoes
- + 1 more ingredients
- 4 teaspoons extra-virgin olive oil
Put oven rack in middle position and preheat oven to 425°F. Oil a 9-inch glass or ceramic pie plate with 1 teaspoon oil. Cook onion with salt in remaining tablespoon oil in a 10-inch heavy skillet over moderate heat, stirring occasionally, until softened and beginning to brown, 7 to 9 minutes. Wh...
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The Weekly Moviegoer - It's Outdoor Movie Season!
by Christopher Campbell
April 27, 2009
It's outdoor movie season! Well, it is for those of us in the north, where we actually experience seasonal changes, and where we have just finally brushed off one of the coldest winters in years. Over the weekend we greeted our first hot temperatures in New York City, just a couple days after the Tribeca Film Festival kicked off the outdoor moviegoing season with a free showing of Teenage Mutant Ninja Turtles. That same night, Rooftop Films, a non-profit organization that presents tons of great indie film screenings on the tops of buildings throughout the summer, held a comedy show featuring live stand-up and short films. Well, it actually took place indoors, but the event was at least a reminder that the outdoor series is approaching.
I attended the TMNT screening, which Tribeca exhibited as part of its non-automobile-friendly "Drive-In" program, mainly because a friend is a huge fan of the franchise. The setting was appropriate, as I could alternate between watching the film playing on the inflatable screen and looking up at the Manhattan skyscrapers surrounding us. I figured that, for a TMNT fan, seeing the movie in the shadow of the city buildings might be somewhat akin to seeing Close Encounters of the Third Kind at the base of Devil's Tower - an experience I wish I could have had when Alamo Drafthouse Cinema's Rolling Roadshow held such a screening four years ago. The only thing more suitable, obviously, would be to show TMNT down in the sewers. And the fans, some of whom wore costumes or at least Ninja Turtles t-shirts, were loving it. You could tell by their excited screams and continued chatter, which often overpowered the audio of the film.
Fortunately, the movie is really silly and hardly well made, so the noise from the audience wasn't too much of a nuisance, yet all that cheering and talking made me realize that I have a real love-hate relationship with outdoor movies. In theory, the idea of sitting outside, often on an uncomfortable ground, braving insects and cold and damp weather (even in the summer) for two hours doesn't sound very appealing. And I'm not even one of those internet writers who never goes outdoors. I love hikes, beaches, beer gardens and other exterior settings/activities. But when watching movies, my preference is for a comfortable seat in a sheltered auditorium. Of course, in theory, because I like so many other outdoor pastimes, I should also appreciate lying on a blanket and drinking beer outside while a good movie screens before me.
Once I'm having the outdoor movie experience, the reality is similar to what I've theoretically expected. The worst part of the TMNT screening, more frustrating than the talking (which moviegoers seem to believe is more acceptable at outdoor screenings), was the cold temperatures we suffered while sitting in a riverside plaza for so long. Though I constantly defend strong air conditioning in indoor cinemas, since heated auditoriums have a tendency to make people fall asleep, and moviegoers can always just bring a sweater if it gets too frigid, I hypocritically cannot stand when outdoor screenings occur on cold nights. I have to admit, though, that even with cold weather, even with rocky grounds to sit on, even with peripheral distractions and, yes, even with loud conversations going on around me, I've always have at least a generally good time at outdoor movies.
As long as the movie doesn't require too much attention and as long as there are friends and other movie lovers in my vicinity, the pros usually outweigh the cons in most outdoor moviegoing experiences. I just have to appreciate that these experiences just happen to be more casual and communal than those had at regular movie theaters.
That said, I don't really understand the appeal of drive-in theaters, which have a kind of outdoor moviegoing experience, lacking much of the community aspect. I understand that they are a good deal economically, which is why drive-in theater business is increasing during the present recession. And I understand the mythology and nostalgia behind them. But otherwise I don't know that I would enjoy the drive-in theater experience if I had access to one. I admit that I'm not a big fan of cars or the culture that revolves around them, so I'm obviously a bit biased. Still, I can't even picture my younger car-owning self being comfortable watching a two-hour movie from the driver's seat of my old sedan. So if anyone has a non-stereotypical, non-mythological, non-financial reason for enjoying drive-ins, please comment below.
Photo from the Tribeca screening courtesy of adeleray on Flickr.
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Castro Valley Union 76
Review for Castro Valley Union 76
Shashi S. on 11/07/2013
Shashi said she received good service from their gentlemenly staff. They are very dependable.
Vehicle: Jaguar X-Type
Category: Service
Service Date: 11/04/2013
Review Created: 11/07/2013
This review was collected via phone interview by Customer Research Inc.


text:
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text:
"A lot of the older generation are dying off. The younger generation don't really know it. You don't see a lot of 20- or 30-year-olds eating it."
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