text:
|Publication number
|Publication type
|Application number
|Publication date
|Jan 13, 1981
|Filing date
|Sep 5, 1978
|Priority date
|Sep 5, 1978
|Also published as
|Publication number
|Henry J. Jeong, Judith I. Blakemore, Nathan Lewin
|Original Assignee
|Bio-Rad Laboratories, Inc.
|Export Citation
|BiBTeX, EndNote, RefMan
|Patent Citations (6), Non-Patent Citations (3), Referenced by (85), Classifications (12)
|External Links: USPTO, USPTO Assignment, Espacenet
1. Field of the Invention
This invention relates to a novel method for a two-site immunoassay.
2. Description of the Prior Art
Two-site immunoradiometric assays for antigens which can bind to at least two antibodies are known. Those known two-site immunoradiometric assays involve a first incubation of unlabeled antibody coupled to an insoluble matrix with the fluid containing the antigen having more than one antigenic determinant, followed by a second incubation with a labeled antibody. Woodhead, Addison and Hales, Br. Med. Bull., Vol. 39, No. 1, 44-49 (1974); Abraham (ed.), Handbook of Radioimmunoassay, Ch. 4 (Marcel Dekker, Inc., 1977).
Such two-site immunoradiometric assays are discussed in Radioimmunoassay And Related Procedures In Medicine, Vol. 1, 131-47, 149-64 (International Atomic Energy Agency, Vienna 1974).
Corning Medical Diagnostics has marketed an assay for determination of human thyroid stimulating hormone (hereinafter referred to as "TSH"). See Corning, TSH (125 I) Radioimmunoassay Test System (March, 1977). In that assay as described by Corning, the radioactive, 125 I antibody, specific for TSH, is added to a known amount of TSH standard or to a patient serum sample. The radioactive, 125 I antibody will bind to any TSH present and form a labeled complex. After a first incubation of equilibration period, another antibody, also raised against TSH, is added to the reaction mixture. This antibody has been covalently bonded to microscopic glass particles and serves to bind the labeled complex formed in the first step. After binding separation can occur by simple centrifugation. The precipitated complex is counted for 125 I radioactivity. A standard curve is constructed in which radioactivity bound to glass is plotted against TSH concentration. The counts bound in the presence of unknown patient samples are compared to the standard curve and the appropriate TSH concentration is determined by interpolation.
Corning also markets an assay for the determination of human thyroxine-binding globulin ("TBG"). See Corning, TBG 125 I Radioimmunoassay Test System (December, 1977). In that assay, an immobilized antibody specific for TBG is added to a known amount of TBG standard or to a patient serum sample. After vortexing, radioactive 125 I labeled thyroxine ("125 I T4") is added to the reaction mixture. According to Corning, the 125 I T4 will then partition between the binding sites on TBG and bovine serum albumin molecules present in the buffer. The TBG can thus become bound both to the immobilized antibody and to the 125 I T4.
The use of antibodies covalently bound to a solid-phase in radioimmunoassays for proteins and polypeptides is shown by U.S. Pat. No. 3,555,143, issued Jan. 12, 1971, to Axen and Wide. Pharmacia has applied that method in a commercial radioimmunoassay for TSH. In this procedure, the antibody is covalently bound to a polydextran. The sample to be determined and the solid-phase antibody are incubated at room temperature for 18-24 hours followed by addition of radioactively labeled TSH and further incubation with agitation for 18-24 hours. The solid phase is separated, washed three times and its radioactivity measured.
The patent application by Monthony et al. Ser. No. 621,197, filed Oct. 9, 1975, describes an immunofluorescent assay method in which the immune reactants are covalently bound to water insoluble hydrophilic polymeric particles of about 0.1-10 microns in size. The solid phase antibody is mixed with the sample containing antigen (or hapten) to be determined and corresponding antigen (or hapten) which has been fluorescently labeled, so as to bind a quantity of labeled and unlabeled antigen. The solid phase is separated and the fluorescence measured by optical spectroscopy, the concentration of the unknown immune reactant being a function of the value of the fluorescence. This application also describes a two-site immunoassay method in which an excess of solid phase antibody is first reacted with the sample containing antigen (or hapten) to be determined, and the complex is subsequently reacted with an excess of fluorescently labeled antibody.
The present invention in an improved two-site immunoassay method in which the sample containing the multivalent ligand to be determined, a labeled receptor for said ligand, and an unlabeled receptor for said ligand covalently bound to a solid-phase support (hereinafter referred to as "solid-phase receptor") are brought together in an aqueous medium to form a substantially stable suspension and in a single incubation mode or step to produce a two-phase system, wherein the solid phase contains the solid-phase receptor, a portion of which has become bound to ligand which in turn has become bound to a portion of the labeled receptor, and the liquid phase contains the unbound portion of the labeled receptor. The solid and liquid phases are separated and either phase analyzed for the labeled receptor, the concentration of which is a function of the concentration of ligand in the sample.
A two-site immunoassay employing a single incubation mode or step provides a significant advantage over assay procedures involving more than one incubation by simplifying, shortening, and rendering more convenient the performance of the assay. This improvement in assay procedure is accomplished while maintaining acceptable assay characteristics such as precision, specificity, and stability, and in addition, is less subject to errors in timing, additions, and other manipulations.
The novel two-site immunoassay of this invention can be applied to any ligand which can simultaneously become bound by two receptors. This group of ligands includes, but is not limited to placental, pituitary, calcium regulating, and adrenal medullary polypeptide hormones, protein and protein fragments; immunoglobulins (antibodies) of various classes; viral, bacterial, and protozoal organisms or particles; enzymes; and tumor-associated antigens. In a preferred example, an assay for human thyroid stimulating hormone is described.
This assay method utilizes a receptor for the ligand, which is any substance which binds the ligand with acceptable specificity and affinity. Accordingly, the receptor may be, for example, an antibody against the ligand. In a preferred example, antibodies are used as the receptor.
The labeled receptor may be labeled with any of a number of known tracers, including, for example, radioactive tags, such as iodine 125 , or fluorescent labels. In a preferred example, radioactively labeled antibodies are employed.
An important component of this assay is the solid-phase support for unlabeled receptor which can form substantially stable suspensions in aqueous media, can be covalently bound to the unlabeled receptor, can be handled conveniently during manipulations such as pipetting and centrifuging, and exhibits low non-specific adsorption properties, or can be treated so that it exhibits such adsorption properties. The suspendability of the solid-phase support on which the unlabeled receptor is immobilized allows for the addition of an excess of solid-phase receptor, which binds the ligand in the sample, and yet permits further participation of the solid phase receptor-ligand complex in reactions with the labeled receptor. A suitable solid-phase support is derivatized polyacrylamide particles in a size range of 0.10 to 10 microns, which provides a substantially stable suspension during the single incubation period.
A preferred embodiment of this two-site immunoassay having a single incubation mode provides high specificity for TSH by using highly purified antibodies. The hormones TSH, HCG, FSH, and LH are each composed of similar alpha and distinct beta polypeptide chains. The hormones FSH, LH, and HCG may interfere with the assay for TSH due to the cross reactivity of the antibodies to those hormones. Improved specificity for TSH of this assay is achieved by a two step purification procedure for the labeled antibody and, if needed, purification of the unlabeled antibody to obtain antibodies of high affinity for TSH and low affinity for other hormones, for example HCG. Prepurification of receptor by these means constitutes an approach in dealing with inherent cross-reactivity due to common antigenic determinants in various subclasses of ligands and constitutes an improvement over prior art. In addition, prepurification of antibody makes it possible to introduce 125 I into a higher proportion of specific antibody molecules (subclass of the IgG fraction), and thus more of the radioactive label is usable in the assay.
The purification procedure for antibody to be labeled involves known principles of affinity chromatography to an immunoglobulin cut of rabbit anti-serum to TSH. In a two-step procedure, the immunoglobulins are first passed over an HCG column to remove anti-alpha chain antibodies, and then over a TSH column. The specific anti-beta TSH antibodies bind to the TSH column, and they are eluted off in stages so that the final antibody for labeling consists of essentially pure anti-beta TSH antiboidies of high affinity. In addition to purification of the labeled antibody, the unlabeled antibody may be evaluated for TSH specificity and, if necessary, either passed over an HCG column prior to coupling to the solid phase support or absorbed with HCG after coupling to the solid phase support in order to provide a specific system. The assay utilizing these purified antibodies does not cross react with HCG, FSH, or LH at normal levels of these hormones, improving the specificity of the assay for TSH.
The FIGURE shows a standard curve.
The assay method of this invention can be applied to the assay of TSH in serum.
The reagents for a single-incubation two-site immunoradiometric assay for TSH are typically prepared as follows:
The antibodies to TSH are produced by injection of TSH into rabbits, according to usual procedures. They are purified according to the principles of affinity chromatography, above. The tracer is prepared by iodinating purified antibody with the isotope 125 I to a specific activity of about 1 to 100 μCi/μg, preferably about 5 to 60 μCi/μg. However, other specific activities may be used. The antibody may be iodinated by conventional methods described in the literature. The tracer may be lyophilized from a solution containing phosphate buffer and carrier protein.
The hydrolysis of polyacrylamide beads and the coupling of antibody to these beads is essentially as described in U.S. Pat. No. 4,088,746, although the hydrolysis may also be carried out at room temperature. The antibody which is coupled is preferably a globulin cut of the antiserum, although whole serum can be used. Usually from 0.25 to 2.0 ml of antibody is coupled to each gram of beads, preferably 0.75 to 1.5 ml per gram. The washing of the coupled beads is accomplished by use of phosphate buffer and 1M ammonium thiocyanate. The beads may be lyophilized from a solution containing phosphate buffer, protein, and HCG, although they can also be left in liquid suspension.
The solid-phase receptor is used at a concentration such that under the assay conditions, the precipitation of TSH is essentially complete. The amount used per assay tube is approximately 1.0 to 5.0 mg, although other amounts may be used.
The standards contain human TSH in human serum-based solution. The serum is stripped of endogenous TSH by charcoal and filtration prior to use. Alternatively, the protein environment of serum may be simulated by the use of dissolved serum or other proteins from human or animal origin. Preferably preservatives are added to the serum, conveniently sodium azide at 0.1% by weight. The human TSH contained in the standards are calibrated against the international reference preparation of human TSH supplied by the World Health Organization (Medical Research Council, Holly Hill, London). However, other standardizations may be used. Preferably the standards are lyophilized and they may be prepared to contain 0, 2.5, 5.0, 10, 25, 50 and 100 μIU/ml TSH, although other levels could be used.
The reaction parameters are typically as described below:
Reaction volumes are kept reasonably small, although larger volumes can be used and the concentrations adjusted accordingly. Typically, 50 μl of tracer with about 50,000 to 300,000 dpm, preferably 100,000 to 200,000 dpm, is added to each assay tube. The volume of solid-phase receptor added to each tube may vary from about 100 μl to 500 μl, preferably 150 μl to 400 μl. The tracer and solid-phase receptor are preferably mixed and a single addition step is employed, but they may be added in two steps with the solid-phase receptor added first.
The incubation temperature is preferably in the range of 2�-50� C., preferably 35�-40� C., the incubation time is conveniently as specified in Example 1, although times of from about one hour to about three hours could be used and acceptable standard curves obtained. Prior to incubation, mixing of the tubes is conveniently done with a laboratory Vortex mixer, although the tubes could also be mixed by manual agitation.
The tracer is conveniently supplied in lyophilized form, although liquid tracer could also be used and appears to give similar stability characteristics. The solid-phase receptor is conveniently bottled and supplied as a suspension, although lyophilized preparations of solid-phase receptor appear to work equally well. The amounts of tracer and solid-phase receptor in each vial are adjusted so that prior to the assay, one vial of tracer could be reconstituted and mixed with one vial of solid-phase receptor. Alternatively, the number of tubes in a particular assay could be determined, and the appropriate amounts of tracer and solid-phase receptor could be mixed in a separate vial prior to the assay. The standards are conveniently supplied in lyophilized form.
Approximately thirty minutes before the assay is to be run:
1. Reconstitute 125 I-antibody to human TSH (tracer) with 2.5 ml distilled water.
2. Reconstitute Zero Standard with 5.0 ml distilled water.
3. Reconstitute standards containing 2.5-100 μIU/ml human TSH with 2.0 ml distilled water.
4. Prepare a normal saline solution (0.9% or 0.154 molar sodium chloride) in distilled water and store at room temperature.
5. Prepare tracer-bead reagent by combining one vial of 125 I-antibody with one vial of solid-phase receptor.
Each reagent must be thoroughly dissolved and mixed with the added water before use. All reagents and specimens should be allowed to come to room temperature before use. The tracer-bead reagent will be a fine suspension of polymeric particles and will appear cloudy. If more than 50 tubes are being run at one time, two vials of tracer should be reconstituted and mixed with two vials of solid-phase receptor in one vial, prior to use in the assay.
1. Label sixteen tubes in duplicate as follows: TC, zero, 2.5, 5.0, 10, 25, 50, and 100 μIU/ml. Label two tubes in duplicate for each patient serum and sample and for each commercial control serum.
2. Add 200 μl Standards zero through 100 to the appropriate tubes.
3. Add 200 μl of each patient's serum or commercial control serum to the appropriate tubes.
4. Mix tracer-bead reagent thoroughly and add 200 μl to all tubes. Set aside TC tubes.
5. Vortex tubes and incubate for two hours at 37� C. (except TC tubes). This step comprises the single incubation mode or step of this invention.
6. Add 3.0 ml saline to all tubes (except TC) and centrifuge for 10 minutes at 1500�g. Immediately after the centrifuge has stopped, decant tubes and blot the tube against filter paper or plastic backed absorbent paper.
7. Count all tubes (including TC) for one minute.
The concentrations of human thyroid stimulating hormone in the patient's samples and commercial control serum are determined from a standard curve. Standard curves may be obtained by several methods, for example, by plotting cpm vs. concentration. FIG. 1 shows a sample standard curve where cpm is plotted vs. concentration. The standard curve must be constructed for each assay, as the actual numbers will vary with the age of the reagents.
Sample data generated using this assay are shown below. These numbers were used to plot the standard curve in FIG. 1.
______________________________________Sample cpm TSH Value______________________________________TC 144062,143313Zero 6094,5464 0 μIU/ml2.5 μIU/ml Standard 9318,9116 2.5 μIU/ml5.0 μIU/ml Standard 11859,11745 5.0 μIU/ml10 μIU/ml Standard 17992,15301 10 μIU/ml25 μIU/ml Standard 25203,25241 25 μIU/ml50 μIU/ml Standard 37266,35871 50 μIU/ml100 μIU/ml Standard 49535,48363 100 μIU/mlCommercial Control I 13187,12766 6.2 μ IU/mlCommercial Control II 19773,19413 14.9 μIU/mlCommercial Control III 24519,25028 23.5 μIU/mlPatient 1 7009,6139 0.8 μIU/mlPatient 2 23466,24102 21.6 μIU/mlPatient 3 8373,7694 1.4 μIU/ml______________________________________
The major use of the present invention from a practical standpoint takes place in clinical laboratories. Typically such laboratories obtain commercial kits containing the various reagents required to perform the assay. Accordingly, the present invention contemplates kits for assaying a specimen for a multivalent ligand having improved reagents permitting the use of a single incubation mode as discussed above. Such kits will comprise containers of: (a) labeled receptor for said multivalent ligand; and (b) an unlabeled receptor for said multivalent ligand covalently bound to a solid-phase support which forms a substantially stable aqueous suspension. Usually the kits will also comprise containers of standards for the multivalent ligand to be assayed. The kits in general may also comprise containers having the various other reagents previously described including those utilized in the preferred embodiment.
|Cited Patent
|Filing date
|Publication date
|Jun 2, 1967
|Jan 12, 1971
|Pharmacia Ab
|Method for the determination of proteins and polypeptides
|Sep 1, 1971
|Dec 10, 1974
|W Dreyer
|Immunological reagent and radioimmuno assay
|Mar 4, 1975
|Nov 30, 1976
|Baxter Travenol Laboratories, Inc.
|Diagnostic reagent system
|Oct 30, 1974
|Apr 12, 1977
|Monsanto Company
|Unitized solid phase immunoassay kit and method
|Mar 28, 1975
|Jul 5, 1977
|Corning Glass Works
|Composite for biased solid phase radioimmunoassay of triiodothyronine and thyroxine
|Nov 11, 1976
|May 9, 1978
|Bio-Rad Laboratories, Inc.
|Radioimmunoassay for thyroid-stimulating hormone (TSH)
|Abraham, Ed, Handbook of Radioimmuno Assay, Marcel Dekker, Inc., N.Y., 1977, pp. 131-134.
|Radioimmuno Assay and Related Procedures in Medicine, International Atomic Energy Agency, Vienna, 1974, pp. 131-147, 149-164.
|Woodhead et al., Br. Med. Bull., vol. 30, No. 1, 1974, pp. 44-49.
|Citing Patent
|Filing date
|Publication date
|Jul 26, 1982
|Sep 11, 1984
|Board Of Trustees Operating Michigan State University
|Method for the detection and/or determination of a polyvalent antigen using at least two different monoclonal antibodies
|Feb 19, 1982
|Nov 6, 1984
|Amf Incorporated
|Pre-precipitated double antibody immunoassay method
|Jun 24, 1981
|Dec 4, 1984
|Hybritech Incorporated
|Immunometric assays using monoclonal antibodies
|Oct 14, 1981
|Jan 29, 1985
|Takeda Chemical Industries, Ltd.
|Method for enzyme immunoassay and production of antibody
|May 21, 1982
|Apr 30, 1985
|The Trustees Of Columbia University In The City Of New York
|Monoclonal antibody mixtures and use thereof for enhanced sensitivity immunoassays
|Nov 9, 1981
|Apr 30, 1985
|Secher David S
|Assay for interferon
|Apr 16, 1982
|Jun 11, 1985
|Jose Carro
|Soluble assay
|May 23, 1984
|Jun 24, 1986
|Research Corporation
|Monoclonal antibodies to vitamin B-6 and immunossay method
|Apr 28, 1983
|Mar 24, 1987
|Pandex Laboratories, Inc.
|Method of solid phase immunoassay incorporating a luminescent label
|Feb 19, 1987
|Dec 20, 1988
|The Trustees Of Columbia University In The City Of New York
|Methods for obtaining monoclonal antibodies useful in enhanced sensitivity immunoassays
|Apr 24, 1984
|Jun 6, 1989
|Centocor, Inc.
|Immunoassay for multi-determinant antigens using high-affinity
|Jun 1, 1987
|Dec 26, 1989
|Unilever Patent Holdings B.V.
|Process and apparatus for carrying out specific binding assays
|May 7, 1985
|Jun 19, 1990
|Nichols Institute Diagnostics
|Delayed solid phase immunologic assay
|Sep 30, 1983
|Apr 23, 1991
|International Immunoassay Laboratories, Inc.
|Two site cross-reaction immunometric sandwich assay method
|Jan 30, 1985
|Apr 23, 1991
|Shah Vipin D
|Two site cross-reaction immunometric sandwich assay method
|May 20, 1986
|Sep 10, 1991
|Agri-Diagnostics Associates
|Kit for diagnosing plant diseases
|May 10, 1988
|Aug 11, 1992
|E. I. Du Pont De Nemours And Company
|Assay device and immunoassay
|Apr 27, 1993
|Dec 20, 1994
|E. I. Du Pont De Nemours And Company
|Solution sandwich hybridization, capture and detection of amplified nucleic acids
|Feb 21, 1992
|Feb 21, 1995
|E. I. Du Pont De Nemours And Company
|Assay device and immunoassay
|Oct 29, 1992
|Feb 21, 1995
|E. I. Du Pont De Nemours And Company
|Method for determining total analyte concentration in a sample having both free and bound analyte
|May 15, 1987
|Jun 6, 1995
|General Hospital Corporation
|Immunoassay utilizing monoclonal high affinity IgM antibodies
|Jul 8, 1993
|Jul 18, 1995
|E. I. Du Pont De Nemours And Company
|Assay with signal detection in the presence of a suspended solid support
|Mar 17, 1993
|Sep 12, 1995
|Ciba Corning Diagnostics Corp.
|Method for detection of an analyte using acridinium esters and liposomes
|Nov 24, 1993
|Dec 5, 1995
|Boehringer Ingelheim Pharmaceuticals, Inc.
|Method for detecting inflammation
|Aug 18, 1994
|Jul 23, 1996
|Ciba Corning Diagnostics Corp.
|Nucleophilic polysubstituted aryl acridinium ester conjugates uses thereof
|Oct 19, 1994
|Jan 21, 1997
|Ciba Corning Diagnostics Corp.
|Method for detection of an analyte using acridinium ester and liposomes
|May 12, 1994
|Feb 11, 1997
|Unilever Patent Holdings B.V.
|Jul 19, 1994
|Mar 11, 1997
|Unilever Patent Holdings B.V.
|Processes and apparatus for carrying out specific binding assays
|Jul 15, 1993
|Apr 22, 1997
|Unilever Patent Holdings B.V.
|Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents
|May 12, 1995
|Aug 5, 1997
|Dade International Inc.
|Assay with signal detection in the presence of a suspended solid support
|May 12, 1995
|Aug 12, 1997
|Chiron Diagnostics Corporation
|Luminescent conjugates for use in luminescent assays
|Sep 15, 1994
|Aug 12, 1997
|Unilever Patent Holdings B.V.
|Test device for detecting analytes in biological samples
|Mar 17, 1993
|Sep 2, 1997
|Chiron Diagnostics Corporation
|Nucleophilic polysubstituted aryl acridinium ester conjugates and syntheses thereof
|Jan 10, 1995
|Mar 10, 1998
|Vanderbilt University
|Method to determine metastatic potential of tumor cells
|Dec 23, 1994
|Apr 14, 1998
|Sinvent As
|Method of assay
|Aug 8, 1997
|Jul 27, 1999
|Dade Behring Marburg Gmbh
|Polysaccharide conjugates of biomolecules
|Aug 29, 1997
|Jun 27, 2000
|Bayer Corporation
|Nucleophilic polysubstituted aryl acridinium ester conjugates and syntheses thereof
|May 20, 1998
|Nov 28, 2000
|Dade Behring Inc.
|Reagents and methods for specific binding assays
|Jun 7, 1995
|Feb 13, 2001
|Conopco Inc.
|Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents
|Jan 13, 1993
|Mar 13, 2001
|Dade Behring Marburg Gmbh
|Assay for bone alkaline phosphatase
|Jun 7, 1995
|May 8, 2001
|Conopco Inc.
|Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents
|Jun 9, 1997
|Mar 5, 2002
|Unilever Patent Holdings B.V.
|Analytical test device for imuno assays and methods of using same
|Aug 7, 1996
|Jun 18, 2002
|Inverness Medical Switzerland Gmbh
|Processes and apparatus for carrying out specific binding assays
|Oct 10, 1996
|Sep 10, 2002
|Luminex Corporation
|Multiplexed analysis of clinical specimens apparatus and method
|May 18, 1999
|Dec 3, 2002
|Dade Behring Marburg Gmbh
|Polysaccharide conjugates of biomolecules
|Jun 18, 1999
|Feb 25, 2003
|Luminex Corporation
|Multiplexed analysis of clinical specimens apparatus and method
|Feb 28, 2001
|Nov 16, 2004
|Inverness Medical Switzerland Gmbh
|Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents
|Jan 31, 2000
|Jan 18, 2005
|Rsr Limited
|Assays for TSH receptor autoantibodies
|Dec 27, 2002
|Sep 6, 2005
|Luminex Corporation
|Multiplexed analysis of clinical specimens apparatus and method
|Jul 2, 2002
|Sep 27, 2005
|Dade Behring Marburg Gmbh
|Polysaccharide conjugates of biomolecules
|Feb 12, 2001
|Sep 19, 2006
|Inverness Medical Switzerland Gmbh
|Sep 4, 2001
|Jul 3, 2007
|Inverness Medical Switzerland Gmbh
|Dec 23, 2002
|Jun 10, 2008
|Inverness Medical Switzerland Gmbh
|Dec 23, 2002
|Aug 5, 2008
|Inverness Medical Switzerland Gmbh
|May 20, 2005
|May 19, 2009
|Bayer Schering Pharma Aktiengesellschaft
|Estradiol prodrugs
|Dec 31, 2004
|Apr 3, 2012
|Luminex Corporation
|Multiplexed analysis of clinical specimens apparatus and methods
|Aug 21, 2002
|Oct 30, 2012
|Rsr Limited
|Epitope regions of a thyrotrophin (TSH) receptor, uses thereof and antibodies thereto
|Dec 12, 2008
|Oct 30, 2012
|Rsr Limited
|Epitope regions of a thyrotrophin (TSH) receptor, uses thereof and antibodies thereto
|Dec 12, 2008
|Nov 13, 2012
|Rsr Limited
|Epitope regions of a thyrotrophin (TSH) receptor, uses thereof and antibodies thereto
|Feb 17, 2015
|Aug 22, 2017
|David Gordon Bermudes
|Compositions and methods for determining successful immunization by one or more vaccines
|Nov 9, 2012
|Sep 5, 2017
|Rsr Limited
|Epitope regions of a thyrotrophin (TSH) receptor, uses thereof and antibodies thereto
|Dec 23, 2002
|Jul 31, 2003
|Davis Paul James
|Dec 23, 2002
|Nov 27, 2003
|Davis Paul James
|May 8, 2001
|Jan 22, 2004
|Walter Elger
|Compounds with sulphonamide group and pharmaceutical compositions containing these compounds
|Dec 27, 2002
|Jun 2, 2005
|Luminex Corporation
|Multiplexed analysis of clinical specimens apparatus and method
|Dec 31, 2004
|Jul 28, 2005
|Chandler Don J.
|Multiplexed analysis of clinical specimens apparatus and methods
|Mar 24, 2005
|Sep 29, 2005
|Ez Bio Inc.
|Reagents, methods and kits for the universal rapid immuno-detection
|May 23, 2005
|Dec 15, 2005
|Ralf Wyrwa
|Estriol and estetrol prodrugs
|Jul 13, 2005
|Dec 22, 2005
|Dade Behring Marburg Gmbh
|Polysaccharide conjugates of biomolecules
|May 20, 2005
|Dec 29, 2005
|Ralf Wyrwa
|Estradiol prodrugs
|Nov 28, 2006
|May 31, 2007
|Gerd Mueller
|Prodrugs of ERbeta-selective substances, processes for their preparation and pharmaceutical compositions comprising these compounds
|Nov 29, 2006
|Jun 14, 2007
|Ralf Wyrwa
|Sulfamoyl sulfonate prodrugs
|Nov 29, 2006
|Jun 14, 2007
|Ralf Wyrwa
|Heteroaromatic sulphonamide prodrugs
|Feb 22, 2007
|Jun 28, 2007
|Scopp Richard L
|Methods and kits for decreasing interferences in plasma or serum containing assay samples of specific binding assays
|Nov 29, 2006
|Aug 23, 2007
|Olaf Peters
|Prodrugs of ERbeta-selective substances, process for their production, and pharmaceutical compositions that contain these compounds
|Dec 12, 2008
|Aug 13, 2009
|Rsr Limited
|Epitope regions of a thyrotrophin (tsh) receptor, uses thereof and antibodies thereto
|Aug 12, 2008
|Feb 18, 2010
|Ez Bio Inc.
|Reagents, Methods and Kits for the Universal Rapid Immuno-Detection
|Jun 10, 2010
|Jun 9, 2011
|Abbott Laboratories
|Methods and kits for decreasing interferences in plasma or serum containing assay samples of specific binding assays
|Nov 12, 1987
|Oct 12, 1993
|Abbott Laboratories
|Determination of analytes in particle-containing medium
|Mar 25, 2005
|Jun 6, 2012
|Reagents, methods and kits for the universal rapid immuno-detection
|Sep 28, 1983
|Apr 18, 1984
|Serono Diagnostics Limited
|Immunoassay of antigens
|Feb 22, 1984
|Aug 30, 1984
|Covalent Technology Corp
|Insoluble surfaces treated to inhibit non-specific protein binding
|Apr 13, 1984
|Nov 22, 1984
|Univ Columbia
|Immunoassay for human chorionic gonadotropin
|Jul 29, 1985
|Feb 13, 1986
|Shah Vipin D
|Two site enzyme labeled cross reaction immunometric sandwich assay method
|Mar 4, 2016
|Oct 27, 2016
|Sanaria Inc.
|Serum antibody assay for determining protection from malaria, and pre-erythrocytic subunit vaccines
|U.S. Classification
|436/500, 436/533, 436/534, 436/808, 436/532, 436/804, 422/400
|International Classification
|Cooperative Classification
|European Classification


text:
GraphicRiver Me Coffee Logo 5429429
Logo Template \ Food
Coffee \ Professional \ Vector \ Shapes \ Details \ Caviar \ Illustriotor \ Dreams
Logo Template - Me Coffee Logo 5429429 by Windows-Vin @ GraphicRiver
Logo Template \ Food
Me Coffee Logo is a Professional logo for you or your company. It's easy to replace the text with your own and then your ready to Print.
- CMYK 300dpi for print and web.
- 100% vector shapes.
- Free font used: Caviar-Dreams
- Link : Font Link Include Text File in Download Folder
- Files.
- Illustriotor Ai,EPS 10 file.
- 1 text help file.
Layered: No; Graphics Files Included: Vector EPS, AI Illustrator; Resolution: Resizable; Minimum Adobe CS Version: CS.
Aivuner Transport Logo; Neo Real Estate Logo; Airline Travel Transport Logo; Writers Rocket Logo:.
Keywords: bakery, black, brown, business, cafe, coffee, coffee logo, cold, creative, cup, drink, food, hot, hotel, me, modern, personal, professional, restaurant, service, shop, simple, vector, web, white.

subset: commoncrawl


text:
100% Wool. This warm yet light wool batting is perfect for projects and garments. It quilts magnificently by hand or machine and allows for quilting up to 3 inches apart. Washable, breathable, and resin bonded, it was developed by Harriet Hargrave for the discriminating quilter. The Tuscany Collection is all washable, resin bonded, breathes naturally.
Cut dimensions: 205 cm x 244 cm
Read the post on this product in our blog
There are no reviews at the moment. Write your review[STOLEG-TW-81 #64620]


text:
Drifting School Trailer (1995)
"Can their adventure into the future change the mistakes of the past?" 01 January 1995 Action, Sci-Fi 84 mins
A spy satellite has malfunctioned and is going to crash into the Earth. The US government bring on line a power laser. When the laser strikes the satellite, it causes a rip and time and sends Monroe High school 200 years into the future, including teachers, students and an escaping criminal. The students find themselves in a barren world, where they are set upon by the escaped criminal and a fierce monster.
Watch the official Drifting School 1995 trailer in HD below or find more Drifting School videos on Vidimovie.


text:
Results 1 to 1 of 1
Thread: automated capavity scripts
09-04-02, 10:20 #1Registered User
- Join Date
- Sep 2002
- Posts
- 3
Unanswered: automated capavity scripts
Hi all,
My company wanted me to write and automate the scripts to report the capacity planning on the tablespace as well as on the OS side. We run Oracle 8.1.7 on NT
any advises???


text:
School Committee Text Size
Event takes place on February 07, 2013 at 5:30 PM until 6:30 PM
1045 Main Street Holyoke, MA 01040
No action to be taken by School Committee. Holyoke's Early Literacy Coordinator, Andrew Melendez, will provide information to City officials on the city-wide literacy initiative.
The listing of matters are those reasonably anticipated by the Chair which may be discussed at the meeting. Not all items listed may in fact be discussed and other items not listed may also be brought up for discussion to the extent permitted by law.
Posted on February 3, 2013 by EileenP


text:
Carbon, the sixth element – this is the fourth teaser released by Lamborghini, describing its upcoming concept which will be unveiled on September 30, at the Paris Motor Show.
What we do know until now, is that the new car which will be unveiled by the Italian supercar manufacturer, is a technology demonstrator.
This new concept will preview the upcoming cars from Lamborghini and will be manufactured using carbon fiber in order to be lighter and faster than the current models.
Still, there are rumors saying that Lamborghini already showed two new concepts to the dealers, during a private meeting, dubbed LB715-5 and 83X. The LB715-5 is rumored to be the Lamborghini Murcielago successor, dubbed Jota, which will use a V12 engine delivering 715 hp.
The other car could be a replacement for the Gallardo (which was introduced 7 years ago), but other sources say that it could also be the production version of the Estoque Concept, a Lamborghini sedan.
Regarding the concept which will be unveiled by Lamborghini this week, it appears that it will be powered by a V10 engine, because a smaller engine and a lighter body will be the new Lamborghini philosophy. More details to be announced this week, stay tuned!


text:
Wukong Build Guide by Endon122
Not Updated For Current Season
This guide has not yet been updated for the current season. Please keep this in mind while reading. You can see the most recently updated guides on the browse guides page.
Thank You!
Your votes and comments encourage our guide authors to continue
creating helpful guides for the League of Legends community.
Wukong Build
Ability Sequence
Not Updated For Current Season
The masteries shown here are not yet updated for the current season, the guide author needs to set up the new masteries. As such, they will be different than the masteries you see in-game.
Hi there u people who wanted to see some ideas on wukong builds!
First of all, Id want to sorry for my bad english and Id want to say that this is my first guide. This build have 3 type wukongs. 1st is AD carry, 2nd dps and 3rd is tanky DPS. In my opinion wukong excels in 3v3 (but i hate 5v5 so take it easy :D) so whole match history is in 3v3.
1st build)I take greater marks of desolation, which is must for almost every meelee champ. Just more dmg with skills is better than just dmg runes, cuz if u have dmg runes, then the armor will be taken in counting of final dmg which results in worse result(lol) than with armr pen, if you understand me :D. For seals i take strength seals which may look as foolishness from me, but trust me, it helps cuz 5 dmg isnt small number. Its like 207 gold at the beginning of the game(half of longsword)! In glyphs i take CDR instead of Ionan boots. With quint and masteries it is 10% CDR which is 2/3 of Ionan boots :D, like 600 gold :D- For quint I take Cdr and some pen which is great.
2nd build)Again desolation, again strength but for glyphs I take mana glyphs cuz Wuk have poor mana pool which forces u go back to base after FB in 3v3. This will help u alot. For quints i take this time only pen runes.
3rd build)There is rune build cpmpletely different: 9 health which provides 50 health(+20 from quint). Seals are ofc armor and glyphs are mag resistance. Quints are combinatin of these 3 types.
in first build i take 21/0/9. Someone might disagree with greed, cuz it gives only 200 gold in 3v3 game, but i like it. Also, some1 might disagree with only 2 points in each of 2 lvl masteries, but both exp and mana cannot be forgotten for wukong carry. In 2nd it is the same except for longer buffs instead of gold. This is due to relatively short duration of Ebonmaw, rabid wolf and ghast(ghast is really short but really useful for your W and R.) 3rd masteries are almost completely different:1/21/9. 1 due to exhaust and def due your tank position in your team. Utility is a mus for almost every champion.
Dorans blade: Dorans blade is good choice for damage champs, but Dorans shield is better choice for tank.
Boots of swiftness:AD carry is just carry so dont expect some use in early game in boots of swiftness, but in mid and late game it REALLY helps your ultimate to chase escaping champs and to keep them in distance and also helps your W to get away faster or to chase stealthed low-health champs. I dont take it for any other build, just cuz in these u are not carry so leave it to some champion with better chase mechanic.
Ionan boots of Lucidity:these boots are good to spam ulti more.
Ninja tabi:Good item for tank.
Sword of the occult:Just ad carry. Ask your teammates to leave you the kills.
Phage:really good:slow and dmg and cures your squishiness.
Sheen:A must for wukong. really good burst dmg in combo described below.
Vampiric scepter:life steal is always nice :P definitely ake it before b f sword.
The brutalizer:really nice. armr pen, CDR and atk dmg. All u need.
Trinitry force:finish it. At this point, your main build is done.
I prefer those items that are seen, but you can of course change your build depending on situation. Buy mercurys treads and cloak and dagger on 1st or 2nd build or buy eleisas miracle on 3rd build. You can also put frozen heart if your opponents have at least two of these: master yi, xin zhao, jax.
TO BE COMPLETED (based on comments so please add some tips :P)
Summoner Spells
First I need to explain you, why I love cleanse for wukong in tanky dps build. You know, whenever you use ulti you can be easily CCed and your ulti is gone. From this point you are probably at the center of enemy team. This means they will probably focus you. So just use cleanse and escape with decoy to your friends :D Also it is very useful in chasing or escaping when you are exhausted, stunned, etc.
Exhaust-my beloved spell. This is spell I use every game. Chasing, escaping, lowering the effectivity in a fight(very useful against champs like Yi or Tryndamereú.
Flash-escape, chase(flash+nimbus strike= chased down, n0ob)
ghost-again escape, chase :D but also(!) movement along the whole map.
No, really, wukong is REALLY good farmer in creep waves, but he cant take ebonmaw alone eveno on level 11. Just use nimbus strike(5th lvl), 2 attacks and front wave is gone. go to the caster wave and decoy if it is on 5th lvl. It will decimate the caster wave almost as good as nimbus strike
Combo is easy:nimbus, autoattack, immediately use crushing blow and if you are only harassing, decoy out. This will procs in(taking blow and nimbus on 5th lvl with build builded to trinitry force:(using math, dont let your brain be destroyed)
1st build:nimbus strike=240+0.8 bonus AD(lets say you have 10 stacks on sword)=240+(50+30+4+3)*0.8=240+84*0.8=240+67.2=307.2 dmg. autoattack=250% of base damage 92.4=231 dmg+4 from runes+80 from items+3 from masteries=318 dmg.crushhing blow= autoattack+150=318+150=468 dmg.
This results in nice dmg=
1093.2(not couning like 30% reduction from armor)
I dont want to count other builds but they will be like 700 dmg or so.
Lane opponents
Best of alll is caitlyn. Just counter her ulti or her slowing net with decoy!
Other good are just opponents with skill shots:amumu, ashe, blitzcrank, brand, corki, Dr.Mundo, Ezreal, Kennen, LeBlanc, Lee Sin, Morgana, Nidalee, Rumble and urgot
I swear it is true, I just dont know how to put images here :P If u say me, Ill put screens here, I have them in word.
15/3/2(triple kill)
ultimate tip for wukong: Dont go alone! Wukong is stronger when he is with other players, cuz he knocks up all members of the team so your team can focus strongest fed carries or casters!


text:
Home & Garden
Home & Garden
Glam up Your Galley (Without Going Broke!)
Trying to dress up your kitchen décor but aren't sure where to start? Take the quiz to test your knowledge about kitchen decorating.
When you're on a budget, the array of kitchen décor choices can seem overwhelming. What's one way to focus yourself?
choose a professional interior designer
choose a décor style
choose a new kitchen color
What's the first thing to do when picking the décor style that will inform your decorating project?
select a style that lends itself to frugality
read magazines to find out what's hot and what's not
consider your own personality
Why is it important to consider the limitations of your kitchen space when you're decorating on a budget?
Blowing out walls may blow the budget.
Standard dimensions on new cabinets are different from standard dimensions on older-style cabinets.
Putting in new windows will almost certainly destabilize load-bearing walls.
One popular décor style for kitchens is the cottage style. What's it characterized by?
weathered finishes, beadboard and colors from the garden
heavy, dark, well-made furnishings and handwoven rugs
mid-century wood textures and low, clean lines
The Art Deco and Arts and Crafts styles differ in which major way?
The Arts and Crafts movement uses modern industrial production techniques, while the Art Deco style is more handcrafted.
The Art Deco movement uses modern industrial production techniques, while the Arts and Crafts style is more handcrafted.
The Arts and Crafts movement is a national extension of Victorian-era style, while Art Deco rejects the ornamentation of the Victorian era.
Contemporary style differs from Modern design in which major way?
Modern design is a subset of Contemporary design and refers only to work created between the 1940s and '50s.
Modern design uses tone-on-tone colors; Contemporary design favors stark whites.
Contemporary style is softer than Modern style, with more tone-on-tone elements than the stark whites of Modern design.
Is Country Kitchen décor an American take on the classic French Country décor style?
Sort of. French Provincial is a substyle of general County Kitchen décor.
No. French Country design features Louis XIV and Louis XV period pieces, while Country Kitchen décor showcases eclectic regional elements.
Yes. Country Kitchen and French Provincial style are synonymous.
What's one great way to save money on a kitchen decorating project?
reface, refinish or replace your existing kitchen cabinet doors
invest in high-quality, new kitchen cabinets that will last a lifetime
wrap your kitchen cabinet doors in seagrass or other natural-fiber fabrics
You can customize your kitchen cabinets without spending a fortune by doing what?
applying faux-stone for a medieval or gnomish look
adding custom molding or glass inserts to your existing cabinet doors
decorating them with photographs and decals
One of the cheapest, easiest ways to transform a space is to transform its ________________.
ergonomic function
A lot of research has been done on the effects of color on mood. According to Psychology Today, blues are more ______________, while reds are more ______________.
calming, energizing
subtle, dramatic
cold, hostile
Different décor styles feature different color palettes. Mediterranean style, for instance, takes its color palette from _________________.
the sea and sky
the browns and golds of rough, inhospitable terrain
the natural fibers woven from native grasses
Colors in the Country decorating style often feature ____________________.
bright glossy finishes
animal inspired textures
milk-paint finishes
No matter how well-dressed your kitchen is, it will never look its best if it's what?
dark or dank
pasted with refrigerator art
In kitchens, there's often a lot of wasted space ________________.
on top of the refrigerator
in the back of the cabinets
in the pantry
What's one great way to organize pantry shelves?
stacking like items on top of each other
placing tall items on lower shelves and short items on higher shelves
using tiered cabinet organizers
Pots and pans take up a ton of space. Pulling them out of the cabinets and hanging them from walls or ceilings is ________________
a great way to free up space
a surefire way to clutter up your kitchen
something that should only be considered if your cookware is of professional quality
Which is the most appropriate type of lighting for a kitchen?
both of the above
more stuff
We'll Give You Three Cities, You Tell Us The State!
You Have One Sentence to Name These Engine Parts. Let's Rev Up and Go!
We'll give you three characters, you give us the '60s TV show
95% of People Can't Name Farm Equipment From an Image. How Will You Do?
89% of people can't name all of these Looney Tunes characters from a single image! Can you?
91% of people can't figure out these famous slogans! Can you?
more stuff
Design a House and We'll Tell You How Old You Are!
81% of People Can't Guess What These Commons Items Are Called in English! Can you?
How Well Do You Know TV Westerns?
Only 1 in 48 People Can Recognize All of These Cursive Letters! Can You?


text:
Australian Small Winemakers' Show 2015
Viognier is delicious with creamy chicken, rich seafood or pork – in fact, almost any dish.
Serve cool not chilled, around 10-13°C. Alcohol:12.9% Cellaring: 2025
Viognier may have originated in Croatia but most people believe its true homeland is the Rhône Valley of France. The world-famous Condrieu region has produced this wonderful, aromatic variety since Roman times and a good Viognier from Condrieu can fetch hundreds of dollars.
This is another variety that was almost lost forever because it is more difficult to cultivate and yields can be unreliable. After the fall of the Roman Empire, Viognier vines almost disappeared. They flourished again from medieval times and then suffered an almost complete wipeout during the World War Two. After the war, only around 40 hectares of Viognier remained, however the variety is again flourishing along the Rhône around Condrieu. Good drainage is essential to the production of excellent Viognier and that is one reason this variety does well on the Granite Belt. The Wild Yeast is the natural bloom on the grapes in the vineyard.
RRP $40 – Inner Circle members: $30


text:
Posts by Jane Adams
George Town, PENANG: Heritage Hotel Capital
Penang has been named as the premier 2017 Asian 'Must See' destination, singled out its street food and art scene. Jane Adams gives her vote to the island's capital George Town, for its wonderfully preserved heritage hotel.Read More
Discover JAPAN's Private Art Collections
Some of Japan's most enthralling art collections are private, and finding them can prove challenging, especially when directions are in Japanese and nailed to lamp posts. But if you like your Picassos, Van Goghs and Renoirs with a little adventure, be sure to visit these off-grid museums.Read More
On the Waterfront, SHANGHAI
Cosmopolitan China is epitomised in Shanghai's Bund riverfront precinct. Jane Adams strolls its boulevards and boardwalk discovering arts, eats and other oriental treats.Read More
CAPITAL CLASS: East Hotel, CANBERRA
Australia's capital city boasts a sleek new hotel packed with soul, style and clever contemporary comforts.Read More
GIBBS FARM: Sculpture by the Sea, New Zealand
Rolling verdant green farmland is the setting for an extraordinary private contemporary sculpture collection just north of Auckland.Read More
A Taste of ORANGE: Regional Food & Wine Trails in Australia
Get the inside track on the best that NSW has to offer visitors keen to experience Australia's local farm produce and vibrant rural communities.Read More
CAMBODIA and Its Culinary Secrets
Award-winning food and travel writer, Jane Adams, shares a fleeting slice of Cambodian life on a plate and place tour through her favorite local hotspots.Read More


text:
Category:Dames Grand Cross of the Order of St John
|This is a category listing, which serves as an index of existing Wikipedia articles about recipients of the Venerable Order of Saint John.
It is not intended to be an exhaustive listing of all recipients.
|Wikimedia Commons has media related to Dames Grand Cross of the Order of St John.
Pages in category "Dames Grand Cross of the Order of St John"
The following 29 pages are in this category, out of 29 total. This list may not reflect recent changes (learn more).


text:
Vox Populi Trailer (2010)
San Cristobal mayoralty candidate Connie de Gracia (Irma Adlawan) is hearing mass, in the hopes that her fealty with the Catholic God translates to votes. After all, the voice of the people is the voice of God. If one is assured of God's undying support because of her utmost religiosity and sincerity, then the votes will simply follow. However, politics in the Philippines is not as divinely blessed as one would wish.
Watch the official Vox Populi 2010 trailer in HD below or find more Vox Populi videos on Vidimovie.


text:
FREE SHIPPING on orders of $85 or more
An excerpt from www.HouseOfNames.com archives copyright © 2000 - 2017
Silesia is the glorious birthplace of the distinguished surname Von hoffman. This name is derived from the German word "hof" meaning "settlement" or "farm" and "mann" meaning "man." The name may have originally been born by someone who owned their land as opposed to working someone else's land, or perhaps by the manager, or steward of such an estate.
Von hoffman Early Origins
Silesia, where the family became noted for its many branches within the region. Ancient chronicles first mention Otten Buzwoys Hoveman of Liegnitz, Silesia (1383,) Hans Kosche Hoffman of Liegnitz (1558,) and Nickel Hofeman the belt-maker of Goerlitz (1425).
Von hoffman Spelling Variations
Von hoffman Spelling Variations
Spelling variations of this family name include: Hoffmann, Hofmann, Hoffman, Hofman, Hoeffmann, Hoefmann, Hoeffman, Hoefman, Hofemann, Hofeman, Hovemann, Hoveman and many more.
Von hoffman Early History
Von hoffman Early History
This web page shows only a small excerpt of our Von hoffman research. Another 745 words (53 lines of text) covering the years 1712, 1540, 1627, 1778, 1845, 1827, 1803, 1789, 1616, 1679, 1640, 1692, 1653, 1719, 1683, 1750, 1685, 1715, 1669 and 1716 are included under the topic Early Von hoffman History in all our PDF Extended History products and printed products wherever possible.
Von hoffman Early Notables (pre 1700)
Von hoffman Early Notables (pre 1700)
Notables of the period with the surname Von hoffman were Christian Hoffmann von Hoffmannswaldau (1616-1679), a German poet of the Baroque era; Pieter Hofman (1640-1692), a Flemish Baroque painter; Martin Hoffmann (1653-1719) a German luthier, based in Leipzig; and his son, Johann Christian Hoffmann (1683-1750)...
Another 43 words (3 lines of text) are included under the topic Early Von hoffman Notables in all our PDF Extended History products and printed products wherever possible.
The Great Migration
The Great Migration
Some of the first settlers of this family name were:
Von hoffman Settlers in United States in the 19th Century
Von hoffman Settlers in United States in the 20th Century
Contemporary Notables of the name Von hoffman (post 1700)
Contemporary Notables of the name Von hoffman (post 1700)
Von hoffman Family Crest Products
Von hoffman Family Crest Products
See Also
See Also
Other References
The Von hoffman Family Crest was acquired from the Houseofnames.com archives. The Von hoffman Family Crest was drawn according to heraldic standards based on published blazons. We generally include the oldest published family crest once associated with each surname.
This page was last modified on 28 February 2014 at 08:10.
Sign Up
FREE SHIPPING on orders of $85 or more


text: All students who are planning for their junior and senior years are invited to apply for admission to the Public Affairs Conversation, regardless of major or intended major. The program strives for the broadest possible mix of backgrounds and interests. Applications will be read by faculty members of the program's steering committee, and the cohort of 20 students for each academic year will be notified of their acceptance during the period of web registration.


text:
Brand Ball Grinding Mill
Marcy Ball Mill Manufacturers,Crusher Mills, Cone Crusher ...
ball mill manufacturers « Professional Crusher Manufacturer. is a ball mill manufacturers in China great selection of used ball mills for mining sites from top manufacturers including Allis Chalmers, Denver and Marcy.:::::Read More
Ball Grinding Mill,eBay
Find great deals on eBay for Ball Grinding Mill in Metalworking End Mills. Shop with confidence.Read More
Make Black Powder Without a Ball Mill,Easy 1 Hour Method
Make your own black powder-aka gun powder-without expensive tools or a ball mill. Homemade blackpowder for a fraction of the cost of buying commercial BP.Read More
India Ball Mill, India Ball Mill Manufacturers and Suppliers ...
India Ball Mill, India Ball Mill Suppliers and Manufacturers Directory,Source a Large Selection of Ball Mill Products at Mine Mill, Grinding Equipment from India Alibaba.comRead More
2040 Ball Mills,
A ball mill grinds ores and other materials to a typi- ... Another stage of grinding follows the mill Reduction ratio is small ... 22 ft. x 36 ft.- 12,500 HP Ball Mill 18 ft. x 22 ft.- 5,500 HP Ball Mills . Title: 2040 Ball Mills Author:Read More
grinding mill
You can obtain all the information about the name, brand, type, ... Jaw Crusher is the most common equipment of the stone crushing, ... Ball mill is an efficient tool for grinding many materials into fine powder.Read More
grinding ball mill Price,AliExpress.com
Grinding Ball Mill Price Comparison, Price Trends for Grinding Ball Mill as Your Reference. Buy Grinding Ball Mill at Low Prices on Aliexpress.com now. Other Price Comparison, such as Industry & Science Price, Home ImprovementRead More
China ball mill grinding balls Manufacturers, China ball mill ...
China ball mill grinding balls manufacturers, China ball mill grinding balls manufacturers & suppliers list, find qualified Chinese ball mill grinding balls manufacturers, suppliers, factories, traders & wholesalers quickly on Made-in-China.com.Read More
Tube Style with Ball Grinding Mill, View Ball Mill, ZK Brand ...
Tube Style with Ball Grinding Mill,US $ 50,000,980,000 / Set, New, Ball Mill, AC Motor.Source from Henan Zhengzhou Mining Machinery Co., Ltd. on Alibaba.com.Read More
Ball Mill Grinding and Mining Used Steel Grinding Ball,China
TS100 TS China, Specification of Steel Ball for Ball Mill Grinding and Mining 1.Reliable High Quality . 2.Reasonable Price. 3.Timely Capable Service. 4.Diameter:13mm-150mm 5.High HardnessRead More
Mills from Cole-Parmer
Brand: Cole-Parmer (7) Retsch (39) IKA (13) Show more (5) ... The lab grinding mill is essential to grind, pulverize, ... Jar mills also require separate purchase of jars and grinding media. Analytical and hand mills are ideal for hard and brittle samples.Read More
Brand Ball Grinding Mill
Ball mill,ball mills,grinding ball mill,grinding millshammer ball mill is crucial equipment for grinding after mater.Read More
Popular Ball Grinder Mill-Buy Cheap Ball Grinder Mill lots ...
Buy Ball Grinder Mill from Reliable China Ball Grinder Mill suppliers.Find Quality Ball Grinder Mill Industry & Science,Home Improvement,Grinder,Home & Garden, and more on Aliexpress.comRead More
Top Brand Ball Grinding Mill, Top Brand Ball Grinding Mill ...
Top Brand Ball Grinding Mill, Buy Various High Quality Top Brand Ball Grinding Mill Products from Global Top Brand Ball Grinding Mill Suppliers and Top Brand Ball Grinding Mill Manufacturers at Alibaba.com.Read More
Used Ball Mills: Used Mining, Grinding & Crushing Equipment ...
When you need high quality grinding equipment for mining sites, dig sites and industrial grinding check out our huge selection of used ball mills.Read More
ball mill grinder, ball mill grinder Products, ball mill ...
ball mill grinder, Search tradeKorea.com for ball mill grinder products, manufacturers, suppliers, exportersRead More
rod ball mill, rod ball mill Products, rod ball mill ...
rod ball mill, Search tradeKorea.com for rod ball mill products, manufacturers, suppliers, exportersRead More
Ball mill for sale UK,grinding ball mill manufacturer,price
Zenith ball mill in UK is created for grinding of clinker, gypsum and dry or moist additives to any type of cement. The mill may perhaps operate in either open or closed circuit and with or without a pregrinder to attain optimum overall grinding efficiency and high flexibility in terms of item ...Read More
,Fuller-Traylor Ball Mills
Fuller-Traylor Ball Mills are the most robust design in the industryRead More
2015 Hot Sell ball grinding mill, View grinding ball mill ...
2015 Hot Sell ball grinding mill,US $ 101,9,999 / Set, New, Flotation Separator, Ball Mill, in details.Source from Zhengzhou General Mining Machinery Co., Ltd. on Alibaba.com.Read More
Grinding Mills,
grinding mills. Grinding mills supplied by ... leading supplier of grinding mills under the Traylor brand name since 1902 and many of the largest plants operating today have these world renowned SAG and Ball Mills operating in some of the most ... Ball mills up to 8.23m in diameterRead More
Next Post: vertical svedala crusher prices


text:
Pokemon Pinball: Ruby & Sapphire
Search Questions
|Answered Questions
|How do you erase your save file?
|How do I solve (team aqua cave)?
|Can I choose to play in English in the German version?
|Johto Starters - are they available?
|My Hi-Score and PokeDex data is missing?
- Total Answered Questions: 6
Visit your Answers Home Page to ask or answer a question for another game.
GameFAQs Answers Expert
You have been recognized as either due to having written a complete FAQ for this game or for having demonstrated an expert level of understanding for this game by answering questions in GameFAQs Answers (or both). The following will now apply when you use GameFAQs Answers for this game:
- Your responses will be highlighted with a label describing your status ("Expert", "FAQ Author", or "Expert / FAQ Author" if you are both).
- You now have the ability to accept an Answer as the correct Answer for any Unresolved Question. You can either choose from an existing Answer or provide a new one if necessary. To do so, simply click the "Accept this Answer" button found below the proper Answer.


text: My Droid has been connected and running fine on my home network since I got it 3 days ago. However, now for the past couple of days I have noticed that the phone wont do anything when connected. When connected to other networks its fine and other devices are fine on my home network, but with the phone, nothing. Is this common? Is there a fix? Running stock 2.2 with a Chevy kernel.


text:
|17 Jan 2014
Windows XP backup with Live One Care
I don't know if this is the place to ask, but I have some old Windows Live One Care backup discs that I could never get Live One Care to open because it wouldn't install on my XP computer, but if anybody has any ideas on where to look for information, I would appreciate it.
|My System Specs
|17 Jan 2014
Windows Live One Care has been superseded by Microsoft Security Essentials, so it is probably a waste of time trying to use it as there are no new updates. You can download Microsoft Security Essentials free from Microsoft & install it.
Refer to this website for more info. Windows Live OneCare - Wikipedia, the free encyclopedia
Hope this helps.
|My System Specs
|27 Jan 2014
Windows Live One Care
The reason I am looking for a way to decipher a Windows Live One Care backup is that I lost some files in a computer crash and some of them are on Windows Live One Care backup discs that I can't access now. I don't think Microsoft Security Essentials would address those. Thanks for the suggestion though.
|My System Specs
|27 Jan 2014
Have a look at this website which may help you recover those files. There is a lot of info on the site so read through it to find ones on One Care, that seem to apply to your problem.
Microsoft forums - Windows Live OneCare Backup and Restore
Hope you get lucky.
|My System Specs
|Thread Tools
|Similar help and support threads
Windows Live Mail: Backup and Restore Question
I plan on formatting and reinstalling W7 this weekend. Important to me is that my emails aren't lost in the process. It's probably an easy process, but no one has ever accused me of being a wise techie. :o I found this thread:...
|Backup and Restore
using contacts from backup folder in Windows Live Mail
I just had my Windows 7 reinstalled and have installed Windows Live Mail. Prior to doing so I did a full backup to an external hard drive. I did not do any special export for Windows Live Mail. I have copied contacts from the Contacts file on the external hard drive file into the Contacts file...
|Browsers & Mail
Windows Live Mail email backup software
I uninstalled my Acronis TIH 2012 and now have no software to back up my email accounts, personally added email account folders and contacts. I would appreciate it very much if anyone knows of any free email backup software that I can use. It doesn't have to have the ability to schedule backups....
|General Discussion
backup IMAP Windows Live Mail
I would like to backup my Windows Live Mail. I am using IMAP. Now, I do know the distinction between POP3 and IMAP. However, I have something that bother me. If I backup my windows live mail (all my gmail emails) and after that I will erase all my emails in gmail. What will happen after...
|Backup and Restore
Windows Live email backup?
I had a save folder and deleted it somehow so what would be a good way to backup your saved email?
|Backup and Restore
Live One Care safty scanner
I ran the complete scan and it found 17 registry errors. I checked the boxes to create a restore point and fix, but it did not fix them. It came back "user skipped"? Any idea what I need to do to repair these registry errors (if anything). 15 of them were file extension something? CCleaner...
|Performance & Maintenance
|Our Sites
|Site Links
|About Us
|Find Us
Windows 7 Forums is an independent web site and has not been authorized, sponsored, or otherwise approved by Microsoft Corporation. "Windows 7" and related materials are trademarks of Microsoft Corp.
© Designer Media Ltd
All times are GMT -5. The time now is 21:07.


text:
payment gateway
luxury beauty brand
Dr. Jart V7 VitaLaser Skin Perfecting Revitalizer is formulated with a 7-vitamin complex to fend off the effects of time while smoothing wrinkles and brightening your complexion. The advanced regenerating ingredients encourage renewal while restoring firmness and improving elasticity. Antioxidants combat free radicals that may lead to premature aging as whitening agent AA2G helps lighten discolorations and freckles.
|Shipping Weight
|3.8 ounces
Disclaimer: All products sold on Zabiva are brand new. We tend to provide accurate information, yet certain information may vary as actual information on the product may differ from the content available on our website. So when you are doubtful, we advise you to read the details mentioned on the product before using or consuming the product. If you require further information on the product we recommend you to directly contact the manufacturer. All the content provided at Zabiva is for informational purpose only and is not intended to diagnose or treat problems. The skin care products are not to cure, and while buying skin care products, customers are advised to check the suitability of the product with their skin type before using the product. We suggest you to consult from the perfect guide before utilizing the product. Hair care treatment products are not intended to entirely diagnose, treat or prevent hair related problems. Zabiva.com undertakes no responsibility for incorrectness of the product. Buy ordering from Zabiva.com, buyers have confirmed to have read this disclaimer.


text:
London, United Kingdom (PressExposure) February 01, 2012 -- TeePee Games, the innovative discovery platform pioneer for social, smartphone, MMO and online Flash games, is pleased to announce that it has partnered with Catching Games to help launch Innebula, a real time strategy MMO game set in a futuristic space setting. The game is currently in open beta and can be discovered through the TeePee Games web portal and Facebook app.
Catching Games is a young, fast growing development studio based in Holland. They formed in 2008 in order to create their first project, the futuristic space MMO Innebula. As a young and ambitious studio, Catching Games' focus is on developing and publishing software that delivers on innovation and creativity, driven by their core values to bring people together and provide a great experience for users.
Innebula is a free-to-play, browser based MMO set in the near future, in which mankind has grown beyond the current borders of Earth and has resorted to space colonisation in order to survive. Players have the choice to become a colonist, and colonise abandoned planets, or a space pirate and assemble the largest fleet in the galaxy.
Innebula's gameplay focuses on strategic elements such as building mines, developing fleets and creating infrastructure for your colony. Players will be provided with missions to help progress in the game, and work towards building flourishing civilisations, with the ultimate goal to become the strongest emperor of space and time.
Anke Kuipers, CEO, Catching Games, Comments, "We're hugely impressed with the TeePee Games discovery platform and we believe that, with their help, Innebula can become a popular and successful MMO that stands out in an extremely competitive market."
Darren Newnham, Head of Content at TeePee Games says, "Innebula is a brilliant addition to TeePee Games' portfolio, and adds to our ever growing collection of quality and enjoyable MMO games. We look forward to having a close working relationship with Catching Games, and can't wait to see what the future holds for them and Innebula."
Innebula can be discovered through TeePee Games at: [http://bit.ly/wMSf0L]
Individuals who would like to find and play fun free Facebook, MMO, online Flash and Android games can visit the TeePee Games website or Facebook application. Developers and publishers interested in having their games featured on TeePee Games can contact Darren Newnham.
For more information contact:
Tony Pearce, CEO
Tel: +44 (0)7775 500847 / @: firstname.lastname@example.com
Darren Newnham, Head of Content
Tel: +44 (0)7919 891451 / @: firstname.lastname@example.com
Quoc Truong, Peppermint P
Tel: +44 (0)207 240 2645 / @: firstname.lastname@example.com


text:
This package is based on the libradius of FreeBSD, with some modifications and
This PECL provides full support for RADIUS authentication (RFC 2865) and
RADIUS accounting (RFC 2866), works on Unix and on Windows. Its an easy way to
authenticate your users against the user-database of your OS (for example
against Windows Active-Directory via IAS).
WWW: https://pecl.php.net/package/radius
make generate-plist
To install the port: cd /usr/ports/net/pecl-radius/ && make install cleanTo add the package: pkg install pecl-radius
cd /usr/ports/net/pecl-radius/ && make install clean
pkg install pecl-radius
PKGNAME: pecl-radius
SHA256 (PECL/radius-1.4.0b1.tgz) = 97dcf2e497131a20804cc508873a123012b773f42d077af398334f3caf86ee54
SIZE (PECL/radius-1.4.0b1.tgz) = 50953
NOTE: FreshPorts displays only information on required and default dependencies. Optional dependencies are not covered.
This port is required by:
No options to configure
Number of commits found: 25
- Update maintainer address
Reviewed by: miwi (mentor)
Differential Revision: https://reviews.freebsd.org/D10755
Convert all PECL ports to USES=php:pecl.
Sponsored by: Absolight
Differential Revision: https://reviews.freebsd.org/D10281
NMU to version 1.4.0b1, adding PHP 7 support.
Submitted by: Franco Fichtner <firstname.lastname@example.com>
Approved by: Jochen Neumeister (maintainer)
Sponsored by: OPNsense
- Pass maintainership to submitter
Submitted by: email@example.com
- Update WWW: pecl.php.net uses https://
Approved by: portmgr (blanket)
Don't use extension.ini any more, and have each extension install in its
file, so the order remains the same.
Every PHP (or Zend) extension now installs its own .ini file in
/usr/local/etc/php. A PHP extension will be automatically activated
when installed. The order into which extensions are loaded is
automatically guessed. In some very rare cases, the guess will be
wrong, and PHP_MOD_PRIO will need to be set. Refer to the USES=php
section of the Porter's Handbook for more information.
Convert ports touching etc/php/extensions.ini manually, or telling the
OP to do it.
And finally, bump PORTREVISION for all php extensions.
Submitted by: mat
Sponsored by: Absolight
Differential Revision: https://reviews.freebsd.org/D7022
Reset miwi@'s ports, he stepped down from the Ports Team.
With hat: portmgr-secretary
Convert USE_PHP*=yes into USES=php*
Submitted by: mat
Exp-run by: antoine
Sponsored by: Absolight
Differential Revision: https://reviews.freebsd.org/D6936
net/pecl-radius: 1.2.7 -> 1.3.0
Submitted by: Franco Fichtner <email@example.com>
Approved by: miwi (maintainer)
- Ignore php70
- While here remove old php gruft
- Take Maintainership
Approved by: mat (mentor)
1: add LICENSE.
2: switch to USES=tar:tgz
Cleanup plist
Reset miwi's maintainership per his demand
Hope to see you back! Thank for all the work!
- Update to 1.2.7
- Pet portlint
Submitted by: Bartek Rutkowski <email@example.com>
- Stage support
Fix NO_STAGE attribution
Add NO_STAGE all over the place in preparation for the staging support (cat:
Fix for php 5.4.
Set IGNORE_WITH_PHP and DEFAULT_PHP_VER.
- Get Rid MD5 support
- Update to 1.2.5
- Take maintainership
Reset maintainership of ports assigned to firstname.lastname@example.com after numerous
maintainer-timeouts and no response to email. We hope to see him back.
Hat: portmgr
Approved by: krion@
New port: security/pecl-radius Radius client library
This package is based on the libradius of FreeBSD, with some modifications and
This PECL provides full support for RADIUS authentication (RFC 2865) and
RADIUS accounting (RFC 2866), works on Unix and on Windows. Its an easy way to
authenticate your users against the user-database of your OS (for example
against Windows Active-Directory via IAS).
PR: ports/78638
Submitted by: Antonio Carlos Venancio Junior <email@example.com>
Servers and bandwidth provided byNew York Internet, SuperNews, and RootBSD
No vulnerabilities have been reported in the past 14 days
* - modified, not new
All vulnerabilities


text:
Yongguk oppa: even if the fan sign has ended, please tell me the most sexy thing that will keep my heart fluttering! <3
BYG: Oppa's hoodie feels warm
BAP Bang Yongguk
he makes me feel reminiscent of a real life crush
he kinda has that sunbae crush feel to him
if he says so, I will go inside Yongguk oppa's hoodie
source: here
김남준(Rap Monster)
wow.. I can imagine myself going in his hoodie..
when I have boobs padding, it feels pretty warm too!
ha........this is the real heart fluttering
omg oppa let me jump into your arms
ha Yongguk oppa ILY!!!!!ILY!!!!!!I told you ILY!!!KYAHHHHHHHHHHHHH
oppa I'm cold...
my heart hurts....ㅠㅠㅠㅠㅠㅠㅠㅠㅠbooh
he sounds pretty casual but that's what makes it so heart fluttering..
I'm warm now since I went in your hoodie a while ago ^0^


text:
Category: Stock Graphic, Objects
Stock Graphic >> Object - Office Desk with Personal Accessorie 328287 by robuart @ CreativeMarket
File Size: 2.27 MB; Layered: Yes; Vector: Yes.
Work process concept with top view of office desk with smartphone, paper documents and personal accessories of businessman in flat design.
Concepts are easy to customize and resize. Vector illustration. All design elements included in EPS file. You can render any resolution JPG or PNG image from vector file.
Zip file contains: EPS10 and high resolution JPG format files.
Buy Now ~ $5
Office Desk with Personal Accessorie
by robuart
Keywords: work process, office, desk, workflow, vector, concept, flat, symbol, analysis, document, clipboard, mobile phone, smartphone, report, book, pen, analytics, bar graph, paper, process.


text:
I specialise in working with organisations that seek positive change in the world. For over 15 years I've worked on a wide array of digital projects incorporating all aspects of film production to the finer details of web development. Inbetween all that I've also picked up many complimentary skills from simple design work to creating wider digital strategies.
My aim is to use those skills to help people communicate their message effectively while picking up more skills on the way.
Some of the stuff on offer: Directing, Producing, Video Editing, Filming/Shooting, Photography, Sound Recording, Script Writing, Copy writing, Motion Graphics, Photography, Graphic Design, Image editing/manipulation, Web Design & Dev, Digital / Social Strategy,HTML, CSS, PHP Code Hacking
In 2010 I went to work for a international development charity Tearfund and my view of the world was changed entirely. Since then I've sought to work with organisations and on projects that have a positive change on the world.
A guy called Jesus once said that one of the most important things to do is to "Love your neighbour as yourself." This combined with my experience at Tearfund has led me to conclude that my neighbours are anyone in the world and to love them is to do everything in my power to ensure they are not suffering through, war, poverty, abuse, illness or other negative impact.
My faith and my young family drive me to never give up in seeking out this positive change in the world.
Mark is an excellent, creative talent that rises to the challenge of meeting market demands for varied and inspirational media and marketing. I hired Mark for various projects including logo design, web design and development, brochure design, trade stand design - in all cases Mark exceeded our expectations and delivered a competitive service.
Generous. Concrete. Flexible. Mark humbly helped us through the creative process. We were delighted with the end result and hope to partner on future projects.
Mark is able to guide our film projects well, explaining processes as he goes along, and engaging spokespeople to get the best out of them. I've found him a skilled and competent film professional to work with
Thanks to Mark, we can now deal with the growing demands and everyday functions of Babbage Construction much better. We are looking forward to continuing working with him in future. Really recommend his expertise.
Mark is technically excellent and has real creative flair. He was able to assess the project, set realistic goals and deliver on time and within budget. We won't hesitate to use him again.
Mark was a privilege to work with and we look forward to working with him again in the future.
Mark has an eye for seeing what others don't see and an ability to turn that into strong visual communications. His experience has given him a varied, all-round portfolio of skills which enable him to effectively attend to a wide range of digital creative projects. He is also a good guy to work with.
Mark has produced a few great videos for Transform. He's helped us to capture and communicate our passion and vision for what we do in a way that really fits with our audience. His work is of a really high standard and is beautifully creative. He's been great to work with - he's enthusiastic, entertaining and dedicated to producing great work!
I've worked with Mark for a number of years now. Firstly at Christian Aid when he expertly delivered a number of bespoke web projects for us. On each and every occasion, Mark has delivered superb results. His creativity, attention to detail, project oversight and dedication ensure consistently high value work across a range of communications channels.
Mark has been a great asset to Tearfund's digital presence, working on a variety of projects. He's collaborative, brings fresh ideas and insights, and always challenges us to think outside the box. He also has a great ability to mix technical and creative online, which makes him able to work with people across an organisation. Plus he delivers, which is always helpful. Highly recommended.


text:
In the music industry's eyes, a band can originate from two broad areas of America — the East Coast or the West Coast, and preferably within close proximity to a large city. The mass of land between these two areas, with the exception of some cities as Chicago and Detroit, is void of any musical talent. To music executives, that's just the home of cornfields and cows, and that's it. And Ester Drang, the Tulsa, Okla.-based rock band, is just another band from the void that proves that too many music execs are off point.
Ester Drang embraces the melancholy tones of the so-called "avant rock" genre, but with a hopeful, introspective tone instead of the common mopey whine of suburban kids. "No One Could Ever Take Your Face" laments, "Whatever you do, let it get to you, tomorrow's a new day, let it get in your way, everything you do becomes a part of you."
The album sounds like one extended track but not because it drags. Every instrument, from slow-rock guitars to electronic beats of synthesizers, blend together so well that the transition between songs is unnoticeable. Another highly redeeming quality of Ester Drang is the lack of ego, especially on the part of singer Bryce Chambers. Chambers' soft, wispy voice loses itself in the assortment of guitars, bass and piano, allowing each instrument to overpower the vocals at times while they build up to their own emotional climax. Chambers brings his voice to the front at times, but lets it lay low for the majority of the record, demonstrating a rare modesty.
Ester Drang has the achieved the difficult task of inflecting classical sounds with synthesized beats to create a modern brand of classic. Infinite Keys retains the ethereal feel of their first full length, Goldenwest, put out on Burnt Toast in 2002. But their latest effort has a coherent connection that gives the music universal appeal, able to relate to the reality of a quiet farm life in Indiana as well as to the reality of the unforgiving concrete of New York City.


text:
[n] a short verse said or sung by a priest or minister in public worship and followed by a response from the congregation
More below...
poem, sursum corda, verse form
\Ver"si*cle\, n. [L. versiculus, dim. of versus. See {Verse}.] A little verse; especially, a short verse or text said or sung in public worship by the priest or minister, and followed by a response from the people. The psalms were in number fifteen, . . . being digested into versicles. --Strype.


text:
Nuti, A., Orives, R., Borini, G., and Filho, O., "Chevrolet Montana - Handling Preview for a Racecar," SAE Technical Paper 2004-01-3380, 2004, https://doi.org/10.4271/2004-01-3380.
The suspension for a road car focuses in a good compromise between Ride & Handling. When this car is a light pickup, there is also an important compromise between driver and full load condition.For a race car, the suspension system must have a different compromise, were Ride is not considered, but it must be able to keep the tire in contact with the ground, transforming engine power into acceleration, brake power into deceleration and steering inputs in correct corner apex.This paper describes the suspension modification in a production Chevrolet Montana and the methodology used to specify springs, anti roll bars and dampers, which has competed successfully in the 32a Mil Milhas de Interlagos.


text:
Monthly Archives: December 2014
The stories we tell ourselves
Here's one: "I'm too old to make a difference, take a leap, change the game…" (Sometimes, I hear this from people who are 27 years old). This is a seductive story, because it lets us off the hook. Obviously, the … Continue reading
Thank You Very Much…
In 2014, I posted 380 blog posts. My goal for 2014 was to post at least one blog article a day. More than 21000 people across 123 countries read this blog. I would say "unique people," but that's redundant. Each … Continue reading
Maybe next year…
The economy will be going big Your knowledge will reach critical mass Your boss will give you the go ahead (and agree to take the heat if things don't work out) Your family situation will be stable The competition will … Continue reading
Rising sun
Originally posted on 3yetwo:
Some day's are better than others and some days, like today, I just break. Burst into tears for no reason. Run a bath so I can't hear myself cry, sit in the tub with a face…
2014 in review and summay of my blogging
The WordPress.com stats helper monkeys prepared a 2014 annual report for this blog. Here's an excerpt: The concert hall at the Sydney Opera House holds 2,700 people. This blog was viewed about 21,000 times in 2014. If it were a … Continue reading
We Love Loyalty
Loyalty is what we call when someone refuses a momentarily better option. If your offering is always better, you don't have loyal customers, you have smart ones. Don't brag about how loyal your customers are when you are the cheapest … Continue reading
Dissatisfaction guaranteed
Great brands are built on dissatisfaction. After all, if you are satisfied with your Revlon makeup or your Nike sneakers or your iPad, why would you buy another one? Satisfied means done, finished, I don't need any more. In … Continue reading
But it's better than TV
At the local health food store lunch buffet, they offer stir fried tempeh (a traditional soy product originally from Indonesia). I never get it. Not because I don't like it (Okay I'm not a fan), but because there are always … Continue reading
The Economics of Christmas lights
A friend posted on his wall that his neighbors are more advanced having put Christmas lights out. This got me thinking, why bother buying them, putting them up, electrifying them and then taking them down again? After all, the economist wonders, … Continue reading
The places you go to…
Over the weekend I visited one of my favorite places. It didn't matter that I had not been there in a while, or didn't know most of the people I encountered. The second I walked in, heard the noise, saw … Continue reading


text:
The wedding gown. It is one of the most iconic, and memorable pieces from a wedding day. It's timeless, it's beautiful, it's a reflection of you and how you want to look on the most important day of your life. However, choosing the dress may not always be as easy as one would hope. Some brides can try on one dress, others 5 dresses, or possibly 65. Some brides may cry, others may not. Some brides know exactly what they want, when others may choose something completely unexpected. The process is one to cherish; it can be fun, it can be challenging, but embrace your time to find your dress. Although there are different elements to consider before choosing your perfect dress, the infographic below will help you get started! The talented crew at Bridal Book shares 13 wedding gown trends for 2013.
What is your favorite trend of 2013? Do any brides-to-be have a gown that fits into one of the categories above?


text:
Salesforce Developer
We are a health-tech company on a mission to activate the best health outcome possible for every person in the world. We solve hard problems - healthcare is a fragmented industry and we're transforming it by creating an ecosystem through our technology platform that brings together all the players to collectively improve patient outcomes. Our team is entrepreneurial and thrives in ambiguity with a strong commitment to scrappy excellence to drive patient impact. We empower every Outcome Activator to own their decisions and outcomes in service to the mission, while also contributing to a collaborative environment that's communally competitive.
With hubs in Chicago and NYC, we've been growing 100% YoY and will impact half a billion patient visits in 2017, as physicians, life science companies and patients adopt our technology platform to make better health decisions during the most critical moments of care. With 200,000 physicians already utilizing our portfolio of 5 products at their practices, we continue to bring a fresh outlook to our pursuits with humble invincibility, whether you're a new addition or have been hustling with us since the beginning.
Join our family to activate good in the world.
You are accountable for ensuring we're utilizing Salesforce in the most effective way possible – from setup and workflow, to user experience, to integrating additional services and custom development. You will be focused on improving each team's effectiveness and user experience by providing a proactive tool that helps them manage their day. The Salesforce developer position is a highly technical and creative role, focused on developing in our custom Salesforce environment. It's designed to work closely with the other members of the Salesforce team and the Product Management team to understand the business needs, estimate and plan the project work in an Agile environment, collaborate on design and prototype solutions, and develop, test, launch and maintain features that resolve business needs and enable new opportunities.
You have a passion for bringing high quality, unit tested features representing best practices for the platform and solving business needs and thrive in a culture of innovation. Salesforce is the hub of our business process and facilitates the workflow, information and core metrics we need to successfully scale our business.
- 3-7 years experience developing in both Apex and Visualforce. We develop our front end using Visualforce pages as a container, so understanding Visualforce markup is not important.
- Experience in a company environment with JavaScript, HTML, CSS, and integrating SF with web services.
- Experience with the agile software development lifecycle.
- A thorough understanding of SF system limitations and development best practices.
- Experience with a front-end framework is a plus. (react, angular, ember, backbone, etc.)


text:
GraphicRiver Ecology Concept 3159199
Stock Vector \ Conceptual
Vector \ Illustration \ Background \ Package \ Contains \ Ecological \ WindVector
Stock Vector - Ecology Concept 3159199 by PixelEmbargo @ GraphicRiver
Stock Vector \ Conceptual
Vector illustration of Green Ecological Background.
Package contains: EPS (8 version), JPG (5163×3425pixels, RGB ) 100% Vector – Changeable to any size
Layered: No; Graphics Files Included: JPG Image, Vector EPS; Minimum Adobe CS Version: CS.
Cardiogram ; House Layout Planning Concept; Book Icon; Modern Cellphone; Business Concept ; Business Concept; House Layout Planning Concept .
Keywords: alternative, background, bright, color, conservation, design, electricity, energy, environment, environmental, freshness, fuel, generation, green, growth, illustration, landscape, nature, plant, power, recycling, spring, summer, sunlight, symbol, turbine, vector, vibrant, wave, wind.
Download Link


text:
You must register to post or download hacks.
If you are having any issues, shoot us an email, Contact MPGH Support.
As always, have fun and enjoy your stay!
- MPGH Staff
Leave a rep/thanks if i helped.
My one and only skype is Truman.oakes Successfull trades: (14)| Scammed By: (0)I bring the heat.
Originally Posted by uber_tuna
---------- Post added at 07:39 AM ---------- Previous post was at 07:38 AM ----------
Originally Posted by johnny96iv
Originally Posted by Heat V
lol #spamcentral? :P
why did you guys bump this 4year old thread, ffs...
Last edited by HΔSTUR; 08-28-2013 at 12:58 PM.
Because Spam
Originally Posted by -Parabol
why did you guys bump this 4year old thread, ffs...
my post 200º!
bump it for spam.
View Tag Cloud


text: Well I never thought I would be posting on the cruise board, but here I am. After days of begging, I have given in and changed our September 2013 WDW trip to an October 2013 cruise. I really don't like cruises, but going to do it once for the family. I have booked us cabin 8534 for the 5 night Bahama cruise on the Magic. If anyone has any tips I would greatly appreciated.


text:
We are all the by-products of our mistakes and we cannot deny that no matter how much we regret making them, we have indeed learnt a great deal from them. But if you got a chance to go back in time and give your younger self some advice, what would that be? We asked this diverse bunch of young and talented women who've excelled in their profession for one such advice they would like to give their younger self. Their answers were a mix of experience, regret and learning. Read on.
Pallavi Singh, 25, Model
"There is nothing in this world I can't do by myself because the greatest strength I have got from nature is being a woman. I would want my younger self to realise it. The beauty and the blessing of it and carry it with grace and confidence. And to be fearless in this man-centric world."
Krithika Iyer, 25, Actor and Model
"My younger self was a wonderful kid; an enthu cutlet if you will. However, she was incredibly naive and would've been happier if someone were around to tell her the following few things:
i. You're 13, kiddo. Forget about the body hair and the unibrow for now. It'll eventually come off (by some very painful ways at that).
ii. It's perfectly okay to not love/excel at math and science. It doesn't make you a failure. Keep the love for history, languages and the arts alive. You're going to make it big there someday.
iii. People will tell you that you're weird. That's good. Make sure they keep telling you that for the rest of your life.
iv. Your parents are your best friends. Take their advice very seriously and don't be afraid to share things with them.
v. Aspire for bigger and better. Never be satisfied with "good", and never settle for the sake of peace.
vi. When you speak to people, look into their eyes. It doesn't mean you're staring, it only lets them know that you really do care."
Rachel Varghese, 29, Musician
"I would like to tell my younger self to be strong with your held head high, pursue your dreams, have many interests and cultivate them and never stop learning, seeking and creating."
Laila Dalal, 30, Hair and Make-up artist
"I would like to tell my younger self to not sweat the small stuff. Life goes on and whatever happens, happens for the best. Also it will pass so have faith"
Like this article? Also read: Women's Day Special: What CLiQs For These Celebs


text:
Apple's yet-to-be-released iPhone 5 was the second "fastest rising" search term on Google in 2011, while users were also keen to learn about cookies and copyright.
Google has released its annual Zeitgeist report on the most popular search terms of this year.
The royal wedding in April between Prince William and Kate Middleton was the "fastest rising" search term of 2011, followed by "iPhone 5".
Apple was widely expected to launch the fifth generation of the iPhone in October, but the California technology giant instead launched the iPhone 4S, an update on last year's iPhone 4.
There has been further speculation that the iPhone 5 will follow next summer, so it is likely that the term will also feature highly on next year's Google Zeitgeist report.
Other technology terms in the "fastest rising" list included daily deals service Groupon, which is being investigated by the UK's Office of Fair Trading, and Apple's iPad 2, the tablet computer launched in March.
The full list runs as follows:
1. Royal wedding
2. iPhone 5
4. Groupon
5. iPad 2
6. Ryan Dunn
7. Adele
8. Minecraft
9. Rebecca Black
10. Ed Sheeran
Google's top 10 "fastest rising" searches for consumer electronics or gadgets in 2011 was also led by a rumoured Apple product, the iPod Touch 5G.
The fifth generation of the market-leading multimedia player is expected to launch in March 2012, potentially alongside Apple's hotly-anticipated iPad 3.
Also on the list was the HP TouchPad, the tablet computer given a dramatic price cut following sluggish sales, and the ASUS Transformer, a hybrid between a tablet PC and a laptop that has received rave reviews this year.
The full list runs as follows:
1. iPod Touch 5G
2. HP Touchpad
3. Acer Aspire 5742
4. ASUS Transformer
5. Dell Inspiron Duo
6. Acer Iconia
7. Beats Headphones
8. Epson Printers
9. Netgear Router
10. 32-inch TV
Technology also featured in the 'What is..?' list, covering the most popular questions submitted by users.
This included an interest in internet cookies due to the new guidelines on how websites can use them, along with questions on copyright following various legal efforts to fight online and digital piracy.
The list also featured 'What is iCloud?', referring to the new cloud storage service introduced by Apple for iPhone and iPod users in the autumn.
Read the full list below:
1. What is AV?
2. What is scampi?
3. What are truffles?
4. What are piles?
5. What is 4D?
6. What are cookies?
7. What is copyright?
8. What is Zumba?
9. What is iCloud?
10. What is probate?


text:
A Princess in Exile.
Neve Bianchi has always lived in the shadow of the big top. Haunted by her father's death, famed magician Giovanni "The Magnificent" Bianchi, she lives in the background, forgotten and alone. The vanity of the circus' new featured act, Bella Venezia, drags the entire company into decline.
But magic is in the air. Neve makes a discovery that could change not only her fate, but the fortunes of everyone in Lang and Perrault's World Famous Circus. Can she trust Brendan, the charming Prince of Blades? Can she conquer her fears and bring the magic back?
Mirrors and Magic is a steampunk Snow White tale, set in a world of alchemy and mechanical marvels.
We've sent an email with your order details. Order ID #:
Listen on the Audible app or these devices.Launch Audible App
-or-Download Free Audible App
To access this title, visit your library in the app or on the desktop website.
Listen on the Audible app or these devices.Launch Audible App
-or-Download Free Audible App
Audible does not currently have the rights to sell this title in your country.
Please consider another book.Continue Browsing
Please add a payment method to continue.Add a Payment Method
Please edit your payment details or add a new card.
If the problem persists, contact customer service at (888) 283-5051Review My Payment Method
If the problem persists, contact customer service at (888) 283-5051
Please add your billing address to continue.Add My Billing Address
Please select a default credit card to continue.Select Credit Card
It looks like you've already used the specified payment method for a free trial.
Please contact customer service if you think we have made a mistake. (888) 283-5051Start Your $14.95 Membership
If the problem persists, contact customer service at (888) 283-5051
If the problem persists, contact customer service at (888) 283-5051
The Princess Of The Circus And The Evil Queen
Yes! It is an amazing series and I really enjoy each foray into this world. I can't imagine not revisiting each story many times. This was, by far, my favorite installment of this unique series.
Hollie Jackson brought each and every character to life vividly, but I have to say Neve was my favorite character! She grows from a scared little girl, afraid to lose her place with her adopted family, into a strong and vivacious woman at ease with herself and unafraid of anything or anyone :) The accents were truly amazing and the best I have heard so far from any voice actor on Audible! I can't wait to hear what she does next :)
This book made me laugh alot more than I expected it to! The animals were definitely unique characters and good for many laughs.
This series does not need to be listened to (or read) in order as it consists of stories just based in the same world and not necessarily connected to each other via plot or storyline. This is the third book written based in this world. I suggest listening to (or reading) Bitter Cold first though (as I did) because it seems to be the first book chronilogically speaking. I can't wait to listen to more!
Neve has lived with the circus for as long as she can remember. She lost her mother when she was very young and her father died in a tragic accident when she was merely 8. That was ten long years ago and now she is afraid her time with her adopted family is almost up. Her guardian, the owner of the circus, has been avoiding her. She has a secret she is reluctant to share with anyone... They might laugh at her... She might be terrible. And, of course, the reigning Queen of the Circus hates her with a passion. Her father was the greatest Illusionist that ever lived and she is not sure that his tragic life ending accident was truly an accident. She does what she can to keep the Circus running smoothly. Feeding animals, helping people prefect their acts, any odd job she can find, even though nothing is expected of her. Will she be able to stay? Or will her gaurdian kick her out after this season? Is the Circus failing? And if so, why? Can she do anything to bring it back to its former glory? Or is it already a lost cause? What really happened to her father? Could a man that talented really kill himself accident? Can she trust Brendan? Why does the reigning Queen have her so? Will she ever live up to her father's legacy?
***This series is suitable for mature young adult through adult listeners (or readers) who enjoy steampunk fantasy fairytales with a dash of horror, romance & plenty of action and adventure :)
- Nocturnus
Mirror, Mirror On The Wall and the Circus
- Terri


text: So I turn my phone on and it tells me my SD cards might not be any good and I will have to reformat it?? WFT?? Its been in the phone for a year and has like 200 pictures on it. Does this have to do with the 2.1 upgrade and is this the begining of the Eris turning into a piece of CRAP!!! And now anytime I go to try to read the card on my PC it doesn't work... I'm I going to lose all the info on the crad...DAM!!!!!! I have pictures on there I really want... Please help Another strange thing, after I pulled the card to try to read it on my PC, all the pictures for my contacts, and wall papers where still on the phone, all those pictures I was using are stored on the SD card. thanks