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Conceived and designed the experiments: XW AG BC BS. Performed the experiments: XW SB CZ BS. Analyzed the data: XW SB AMW KDW CZ BC BS. Contributed reagents/materials/analysis tools: XW SB AMW PA EK KDW CZ BS. Wrote the paper: XW BC BS. Read and approved the manuscript: XW SB AMW AG PA EK KDW BS.
The SRY (sex determining region Y)-box 11 (SOX11) gene, located on chromosome 2p25, encodes for a transcription factor that is involved in tissue remodeling during embryogenesis and is crucial for neurogenesis. The role for SOX11 in hematopoiesis has not yet been defined. Two genes under direct control of SOX11 are the class- III β-tubulin gene (TUBB3) in neural cells and the transcription factor TEA domain family member 2 (TEAD2) in neural and mesenchymal progenitor cells. Normal, mature lymphocytes lack SOX11 but express SOX4, another member of the same group of SOX transcription factors. We and others recently identified SOX11 as aberrantly expressed in mantle cell lymphoma (MCL). Since SOX11 is variably expressed in MCL it may not be essential for tumorigenesis, but may carry prognostic information. Currently, no specific functional effects have been linked to SOX11 expression in MCL and it is not known which genes are under influence of SOX11 in lymphoma. In this study we found variable expression of SOX11, SOX4 and SOX12 mRNA in mantle cell lymphoma cell lines. Downregulation of SOX11 expression by siRNA verified that SOX11 controlled the expression of the gene TUBB3 in the MCL cell line Granta 519. Furthermore we identified, by global gene expression analysis, 26 new target genes influenced by siRNA SOX11 downmodulation. Among these genes, DBN1, SETMAR and HIG2 were found to be significantly correlated to SOX11 expression in two cohorts of primary mantle cell lymphomas. Chromatin immunoprecipitation (ChIP) analysis showed that these genes are direct targets of the SOX11 protein. In spite of almost complete downregulation of the SOX11 protein no significant effects on Granta 519 cell proliferation or survival in short term in vitro experiments was found. In summary we have identified a number of genes influenced by SOX11 expression in MCL cell lines and primary MCL. Among these genes, DBN1, SETMAR and HIG2 are direct transcriptional targets of the SOX11 protein.
The SRY (sex determining region Y)-box 11 (SOX11) transcription factor was recently discovered as a new marker in mantle cell lymphoma (MCL), expressed in both cyclin D1 positive and negative cases [1], [2], [3], [4]. SOX11, located on chromosome 2p25, is a member of the SOX gene family and was discovered in 1995 [5]. Approximately 20 SOX genes have been identified and they are divided into eight subgroups according to the degree of similarity within and outside the high mobility domain (HMG) [6]. All SOX genes have unique and specific expression patterns and they control cell survival, proliferation and differentiation in numerous processes during embryogenesis (reviewed in [7]). SOX11, together with SOX4 and SOX12, belongs to the SOXC group, sharing a high degree of homology both in the HMG domain and the C-terminal transactivation domain [8]. In the mouse, Sox11 is important for organ development, neurogenesis, neural cell survival and neurite outgrowth [8], [9], [10] [11] while Sox4 is crucial for B lymphocyte differentiation [12]. Upregulated SOX11 has been found in brain tumors and in ovarian carcinoma, where it has been associated to tumorigenesis and clinical outcome [13], [14], [15], [16], [17]. The recently reported aberrant expression of SOX11 in most MCL [1], [2], [3], [4], [18], [19] raises the hypothesis that SOX11 may have a role in the pathogenesis of MCL.
MCL is characterized by enhanced cell proliferation, impaired cell death pathways and reduced response to DNA damaging agents and is therefore difficult to treat [20]. These features can only be partly explained by the t(11;14)(q13;q32) causing dysregulation of cyclin D1 expression. While the t(11;14) is an early event in the pathogenesis of most MCL other genetic events are necessary for lymphoma development [21], [22].
The class-III β-tubulin gene (TUBB3) is up-regulated by Sox11 in neural cells [8]. Using siRNA-mediated knockdown of SOX11 in the MCL cell lines Granta 519 and JeKo, we found that TUBB3 was influenced by SOX11 also in MCL. Additionally, 26 genes were significantly downregulated upon SOX11 silencing in Granta 519. Among these genes, drebrin 1 (DBN1), SET domain and mariner transposase fusion gene (SETMAR) and hypoxia-inducible protein 2 (HIG2) expression correlated to SOX11 expression in a series of SOX11 positive MCL [4]. We found a strong correlation between SOX11 and DBN1, SETMAR and HIG2 expression in a recently published study on SOX11 positive and negative MCL [19]. Furthermore, in MCL cell lines and primary MCL cells, DBN1 significantly correlated to SOX11 expression.
SOX4, SOX11 and SOX12 may have partially overlapping effects on transcriptional regulation [8]. We therefore investigated the expression of the three SOXC transcription factors, SOX4, SOX11 and SOX12 in the t(11;14) positive MCL cell lines Granta 519, Rec1, JeKo and JVM-2 (Figure 1). Granta 519, Rec1 and JeKo expressed SOX11 mRNA and protein while JVM-2 was negative for SOX11 expression (Figure 1). SOX4 and SOX12 protein expression could not be measured due to lack of commercially available specific antibodies. The Granta 519 cell line expressed high levels of SOX11 and very little SOX4 and SOX12 and was therefore chosen for further studies.
The effect of siRNA-inhibition of SOX11 in Granta 519 was investigated using the S13312 siRNA. After 20 hours, the expression of SOX11 mRNA decreased by approximately 85% compared to cells treated with control siRNA (Figure 2A). Downregulation of mRNA was stable between 12–48 hours (data not shown). The SOX11 protein could not be detected at 20 hours after SOX11 knock down (Figure 2B) and the protein levels remained undetectable for 72 hours (data not shown). Similar silencing effects were obtained by another SOX11 siRNA, S224667 (data not shown). TUBB3 has been shown to be regulated by Sox11 in non-hematopoietic cells [8]. We therefore investigated whether SOX11 is able to regulate TUBB3 in Granta 519. 20 hours after transfection with SOX11 siRNA the expression of TUBB3 was reduced by 70% compared to negative control siRNA (Figure 2C).
We next analyzed the effect of knock down of SOX11 on global gene expression in Granta 519 cells. At the chosen time point, 20 hours after transfection, 26 genes were significantly downregulated in SOX11 siRNA treated cells compared to cells treated with control siRNA at the chosen threshold (FDR<0.002, FC≥1.5 or FC≤−1.5) (Table 1). No upregulated genes were found using this threshold.
To test the generality of our findings we investigated how the expression of the 26 downregulated genes correlated to SOX11 expression in 16 primary MCL, all positive for cyclin D1 and SOX11 [4]. In addition to SOX11, nine of the 26 genes were represented on the U133A chip used in this analysis. Of these, HIG2, SETMAR and DBN1 were significantly correlated to SOX11 expression values (Table 2). During the course of our study, Fernandez et al. provided evidence for lack of SOX11 expression in leukemic MCL with indolent clinical course [19]. We therefore investigated possible correlation with SOX11 among these MCL cases (GSE 16455). 19 of our 26 genes defined by SOX11siRNA were represented in this data set. The expression of 15 genes of these genes was significantly correlated to SOX11 expression, including DBN1, SETMAR and HIG2 (Table 3).
To validate the results of the gene expression analysis, SETMAR and DBN1 were analysed by quantitative RT-PCR in Granta 519 after SOX11 siRNA treatment. Both SETMAR and DBN1 mRNA levels were significantly (p<0.001) reduced by SOX11 downregulation in Granta 519 cells (Figure 3A). In another MCL cell line, JeKo, SOX11 downregulation caused similar reduction of TUBB3 and DBN1 levels but not of SETMAR (Figure 3B). We also found a significant correlation between the expression of SOX11 and DBN1 in primary MCL cells (8 samples from 6 patients as described in Materials and Methods and Table 4) and in the MCL cell lines Granta 519, Rec1 and JeKo (Figure 4).
To investigate whether DBN1, SETMAR and HIG2 are direct targets of SOX11 protein, we performed chromatin immunoprecipitation (ChIP) assays on Granta 519 cells using two pairs of primers for each gene as described in Material and Methods. Granta 519 cells were cross-linked, and protein-DNA complexes were immunoprecipitated using antibodies recognizing normal rabbit IgG or anti-SOX11 antibody. We found that SOX11 was significantly recruited to DBN1, SETMAR and HIG2 promoter regions close to the transcription start site (TSS), but not to regions 2 kb distal to the TSS (DT) (Figure 5).
The main finding of this study is the identification of 26 genes influenced by SOX11 in MCL. SOX11 has critical roles in embryonic neurogenesis and tissue remodeling [5], [9], [23], [24]. It is also required for neuron survival and neurite growth [25] but no defined role in hematopoiesis has yet been shown.
The SOX11 protein belongs to the HMG box super family of DNA-binding proteins which are highly conserved between animals. They are involved in the regulation of such diverse development processes as specification of early embryonic germ layers [26], [27], organ development and neurogenesis [28]. SOX11, together with two other SOX family members SOX4 and SOX12, belong to the same subgroup, SOXC. SOX4 is so far the only gene in this family that has been found to have an important role in lymphopoiesis. SOX4–null hematopoietic cells grafted into wild type mice remain blocked at the pro-B cell stage [12]. Although SOX11 is highly homologous to SOX4, its role in lymphopoiesis remains unclear.
Recently, SOX11 was found to be aberrantly expressed in MCL [1], [2], [3], [4], [18]. Importantly, approximately 10% of cyclin D1 positive MCL lack nuclear expression of SOX11 [2], [4], [19]. SOX11 is also expressed in subsets of Burkitt lymphomas, lymphoblastic leukemias and hairy cell leukemias [1], [3], [18]. Thus, expression of SOX11 in lymphoma seems independent of the t(11;14) cyclin D1 translocation.
The possible role of the aberrant SOX11 expression in lymphoma is unknown. Others have shown that SOX11 is needed for the expression of pan-neuronal genes, including the Class-Ш β-tubulin gene TUBB3 (also named Tuj1). TUBB3 was the first gene to be identified as a potential direct target of SOXC proteins. In this study, we showed that SOX11 can modify the expression of TUBB3 also in MCL.
By gene expression analysis using a very stringent threshold, we identified 26 genes, all significantly downregulated, after SOX11 knockdown in MCL cells. Three of these genes were also found to be significantly correlated to SOX11 expression levels in 16 SOX11 positive primary MCL samples from various tissues. During the course of our study, Fernandez et al. published results on MCL with leukemic, non-nodal presentation and a very indolent clinical course [19]. Many of these tumors had in fact been misdiagnosed as other types of lymphomas. 15 of the 26 SOX11siRNA downregulated genes in Granta 519 were found to be significantly correlated to SOX11 expression in the Fernandez cohort, confirming our observations.
Prior to this study, Tubb3 and Tead2 were the only genes proven to be directly regulated by Sox11 [8], [9]. By chromatin immunoprecipitation we showed direct binding of the SOX11 protein to the promoter regions of the DBN1, SETMAR and HIG2 genes in MCL cells.
DBN1 and three other genes downregulated by SOX11 silencing, TMSB15A, TMSB15B and C5orf13 are actin binding and involved in cell shape and motility. DBN1 encodes for the actin binding protein drebrin 1 (developmentally regulated brain protein), cloned in 1988 [29], [30]. DBN1 was later shown to be is involved in neurite formation and synaptic signalling (reviewed in [31]) and to be downregulated in brains of Alzheimer patients [32], [33]. Drebrin is also expressed in epithelial cells of the stomach, kidney, colon and in cell lines from fibroblasts and astrocytoma [34]. Interestingly, drebrin is cleaved by caspase-6 [35], encoded by another of the SOX11siRNA downregulated genes.TMSB15A and TMSB15B encode for different isoforms of the actin binding protein thymosin beta 15. Thymosin beta 15 promotes cell motility and was discovered as highly upregulated in human breast, prostate and colon cancer [36], [37], [38], [39]. According to NCBI, C5orf13 is coding for the protein P311, highly expressed in glioma and involved in glioma cell migration through the reorganization of the actin cytoskeleton [40]. Thus a number of genes involved in cell shape and motility were downregulated by SOX11 siRNA treatment of Granta 519 cells. We could, however, not detect any significant change in shape or growth pattern of the cells after siRNA treatment (data not shown).
SETMAR, HIG2 and HMGB3 could potentially influence cell division and response to cytostatic treatment. SETMAR, also called METNASE, is cooperating with topoisomerase II alpha in decoiling of chromosomes during mitosis. Recent evidence in acute myeloid leukemia suggests that SETMAR may confer resistance to the topoisomerase II alpha inhibitor VP-16 [41], [42]. The HMGB3 protein belongs to the high mobility group of proteins and may, as shown for other HMG proteins, play a role in DNA replication and transcription. Hmgb3 is highly expressed in hematopoietic stem cells [43]. Recent experiment in mice indicate that Hmgb3 downregulation is associated with increased Wnt-signalling, more rapid renewal of stem cells and fewer lymphoid and myeloid progenitor cells [44]. We therefore investigated a possible influence on cell proliferation or cell survival in vitro after SOX11 knock-down by siRNA. In these short term experiments, we did however not detect any significant changes in cell proliferation or viability after SOX11 downregulation (manuscript in preparation), in contrast to what has been reported for neural cells [25]. However, since the effect on gene expression in siRNA treated cells is transient, we cannot exclude possible effects of SOX11 expression on cell survival or proliferation in other experimental settings.
SOX11 is aberrantly expressed in MCL but the molecular mechanism(s) responsible for its upregulation in lymphoid and hematopoietic cells are not yet defined, in contrast to other cell types. In chicken neural cells overexpression of Ngn2 and Ascl1 upregulates Sox11 while the transcriptional repressors Id1 and REST/NRSF downregulates its expression [11]. In the mouse embryonal neuronal plate Sox11 was upregulated by FoxD5 and Notch signalling [45], [46]. SOX11 mRNA expression was also rapidly (within 3 hours) upregulated after exposure of retinal microglia to 17beta-estradiol [47]. Even though SOX11 is highly expressed in most MCL, expression of SOX11 seems not to be dependent on the t(11;14) since it is a feature of both cyclin D1 positive and cyclin D1 negative cases [3]. Furthermore, SOX11 may be expressed in other aggressive B cell lymphomas that lack cyclin D1.
In summary, we have used selective siRNA targeting and downregulation of SOX11 to identify a set of SOX11 responsive genes. Our results have been validated in primary MCL tumors and freshly isolated lymphoma cells. Three of the identified genes, DBN1, SETMAR and HIG2, were found to be directly targeted by the SOX11 protein in MCL.
This study was approved by the Ethical Committe at the Karolinska Institutet, and performed according to the principles expressed in the Declaration of Helsinki. All the patients gave their written informed consent to participate in the study.
Eight samples of tumor cells were collected from 6 patients diagnosed with cyclin D1+ MCL at the Department of Pathology (Karolinska University Hospital Huddinge, Sweden) (Table 4). Tumor cells were isolated from different tissues (bone marrow, blood, lymph node, spleen, pleural exudate), viability frozen in RPMI 1640 medium containing 10% DMSO and 40% FBS and stored at -135°C.
Granta 519, Rec1, JeKo, JVM2 cell lines and primary cells were obtained and cultured as previously described [48]. In short, cell lines were maintained in culture medium (RPMI 1640, supplemented with 10% fetal bovine serum (FBS) and 50 µg/ml gentamicin (all from Invitrogen, Carlsbad, CA)) under standard conditions (humidified atmosphere, 95% air, 5% CO2, 37°C). The cells were maintained between 3×105 and 1.5×106/ml. Culture medium was changed twice weekly.
Two silence ® select pre-designed SOX11 siRNAs, S13312 and S224667, and one non-targeting silence® select Negative Control #1 siRNA were obtained from Ambion (Ambion, Austin, TX). siRNA S13312, used in most experiments had the sense targeting sequence (5′-3′): GACCUGAUGUUCGACCUGAtt. Results were confirmed using S224667 with the sense targeting sequence (5′-3′): GGAGCUGAGCGAGAUGAUCtt. Freeze-dried siRNAs were suspended in nuclease-free water (Ambion, Applied Biosystem, Austin, TX) in a final concentration of 50 µM. Transient transfection were performed on an Amaxa Nucleofection Device (Lonza Cologne AG, Cologne, Germany) according to the manufacturer's instruction. In brief, Granta 519 cells were split at a density of 5×105/ml in the medium 48 hours before transfection. Thereafter, 4×106 cells were collected and resuspended in 100 µl human cell line nucleofector solution C with 100 pmol of either SOX11 siRNA or control siRNA using the X-01 electroporation program. The two SOX11 siRNAs were tested for their individual ability to knock down SOX11 expression. Both siRNA sequences were able to knock down SOX11 expression by 80%-85% in Granta 519 cells.
Cells were harvested 20 hours after siRNA transfection. Total RNA from individual transfection experiments in Granta 519 cells (n=4) or patient samples (n=8) was extracted with Qiagen RNeasy Plus Mini kit according to the instructions of the manufacturer (Qiagen, Valencia, CA). RNA quantity was measured using a NanoDrop (NanoDrop Technologies, Wilmington, DE) set for RNA measurement (A260/A280 ratio). RNA quality was assessed using an Agilent Bioanalyzer 2100 (Agilent Technologies, Palo Alto, CA) and samples with high-quality RNA were hybridized to Affymetrix GeneChip Human Gene 1.0 ST arrays (Affymetrix Inc. Santa Clara, CA) in accordance with the Affymetrix standard protocol at the Bioinformatics and Expression Analysis Core facility (BEA, Department of Biosciences and Nutrition, Karolinska Institutet).
The microarray image data were processed in the BEA core facility according to standard procedures. CEL data was analyzed with Partek Genomic Suite vs 6.5 (Partek Inc., St. Louis, MO) which can normalize and process multiple datasets simultaneously. Data normalization was performed by Robust Multiarray Analysis algorithm (RMA). Statistical differences were examined using one way ANOVA. Significant probesets were defined with a p-value cutoff significant with a False Discovery Rate (FDR) of <0.002, and a fold-change (FC) equal or greater than 1.5(FC≥1.5) for upregulated genes and equal or less than −1.5(FC≤1.5) for downregulated genes, respectively.
Gene expression values for the selected genes were retrieved from two primary array databases. Our previously published gene expression analysis included 16 MCL tumor samples, all expressing cyclin D1 and SOX11 [49]. We also retrieved data from a recently published study from [19] which included 7 indolent, SOX11 negative MCL and 15 conventional, SOX11 positive MCL (http://www.ncbi.nlm.nih.gov/geo/with the GSE accession number GSE16455).
cDNA was generated according to the protocol for Omniscript Reverse Transcription (Qiagen, GmbH, Hilden, Germany). 2 µg of RNA was used in conjunction with Oligo(dT) primer and 20 µl of cDNA was generated. 1 µl of cDNA was added to qPCR Kit Platinum SYBR Green qPCR SuperMix-UGD with FITC (Invitrogen) according to the manufacturer's instructions, run in triplicate on a CFX96 Real-time System (C1000 Thermal Cycler, BIO-RAD, city, CA). The following primers were used: SOX11, 5′-CATGTAGACTAATGCAGCCATTGG-3′ and 5′-CACGGAGCACGTGTCAATTG-3′; SOX4, 5′- CAGCCCCTAATTTCTCCATGTT and 5′- GGTGGCAGGTTAAGGGATACTG; SOX12, 5′- CCCAGGTCCACCCTCAGTAC and 5′- CGAGAGTCTTCCTGCCATCAC; SETMAR, 5′-GCGGAAGCGGCAAAGAC-3′ and 5′-GCCTCAGGCTTCTCCTTAAACTC-3′; DBN1, 5′-GCCCCACCTGCTAACCAA-3′ and 5′-GTGATTGACTGAAGTACCCCTCACT-3′; TUBB3,5′- GGAGCGGATCAGCGTCTACT-3′ and 5′-GCTCGAGGCACGTACTTGTG-3′; β-actin, 5′-AAAGACCTGTACGCCAACACA-3′ and 5′-AGTACTTGCGCTCAGGAGGA-3′. The cycle parameters used was: 50°C 2 min, 95°C 2 min, followed by 40 cycles of 95°C for 15 seconds and 59°C for 30 seconds. Ct values (Threshold cycles) were obtained from amplification of SOX11, SOX4, SOX12, SETMAR, DBN1, TUBB3 and β-actin. The ΔCt value was calculated by subtracting the Ct value of β-actin from the Ct value of target gene. A ΔΔCt value was then calculated by subtracting the ΔCt value for siRNA condition from the ΔCt value for control condition with relative fold increase (RFI) reported as 2−ΔΔCt.
Western blotting was performed as described previously [4]. The blotted membranes were probed with rabbit anti-human SOX11 (HPA000536, Atlas Antibodies, Uppsala, Sweden), and antibodies to tubulin (SC 8035, Santa Cruz Biotechnology, Santa Cruz, CA) or actin (A4700, Sigma, Saint Louis, MO) was used as loading controls. Antibody binding was detected by enhanced chemoluminescence using SupersignalWest Pico (Pierce Biotechnology, Shelton) CTchemiluminescent substrate.
Granta 519 cells were split at a density of 5×105/ml in the medium and cultured for 72 hours. Thereafter, 2×107 cells were collected and crosslinked with 1% formaldehyde for 10 min. Cross-linking was quenched by adding 125 mM glycine and cells were washed with cold PBS, harvested and resuspended in lysis buffer containing protease inhibitors cocktail (Roche, Mannheim, Germany) and sonicated 15 min for three times. The soluble chromatin was collected by centrifugation and the supernatants were incubated with 30 µl protein A/G Sepharose (50% slurry; GE Healthcare Bio-Sciences Corp., Uppsala, Sweden) under gentle agitation over night at 4°C. The supernatant was transferred to a new microcentrifuge tube, followed by immunoprecipitation with 1 µg of anti-SOX11 antibody and non-immune rabbit IgG (Santa Cruz Biotechnology, Santa Cruz, CA) as control at 4 C overnight. Protein A/G Sepharose (20 µl of a 50% slurry) was then added and incubated for 1.5–2 h. The pellets were successively washed with different buffer as previously described [50]. Protein-DNA crosslinks were reversed by overnight incubation at 65°C in 120 µl elution buffer [TE; 1% SDS]. DNA was purified using a PCR purification kit (QIAGEN, Valencia, CA) and eluted in 50 µl of elution buffer. The immunoprecipitated DNA was amplified by real-time PCR using Fast SYBR Green Master Mix (Applied Biosystems, Warrington,UK) Two pairs of primers were designed for DBN1, SETMAR and HIG2 with one primer designed close to transcription start site (TSS) and one distal (~2 kb) to the TSS (DT).
The primer pairs used are as follows:
DBN1 TSS forward: 5′-TGAGGTGGAAGGATGTTTGCT-3′; Reverse: 5′-CGGCGGTAAGGGAGTCACT-3′.
DBN1 DT forward: 5′-CCCTGCCGTGGGAGTCT-3′; Reverse: 5′-TCCCAGAGGAGTCCCAAGTAGA-3′.
SETMAR TSS forward: 5′-GGGAGCCAGACCCAAAAAGT-3′; Reverse: 5′-TTCTCAGGAGTGGCCTGGAA-3′.
SETMAR DT forward: 5′-AGAAAGATACAGAGAAGGGACTACTTGAG-3′; Reverse: 5′-TTCTTGTATCTCCAGTGCCTTTACC-3′.
HIG2 TSS forward: 5′-TCACTCCAGAACACAATGACTCAA-3′; Reverse: 5′-CGCGGAGCTGTTTCCAAA-3′.
HIG2 DT forward: 5′-TTTCCAACTGGCATGACCTTT-3′; Reverse: 5′-GCACCTCTCCAACAATTCTTTCTC-3′.
Quantitative RT-PCR data were statistically analyzed using paired t -test with P values <0.05 as statistical significance. The correlation between SOX11 and other genes as well as the expression differences among MCL were analyzed with the Spearman's rank correlation coefficient test or Pearson correlation coefficient for qRT-PCR results using Origin 8 software (OriginLab, MA USA) using Origin 8 software. 2-tailed test for significance was used.
We are grateful to the personnel at the Flow Cytometry Unit at Karolinska University Hospital Huddinge for the help with collection of patients' samples and flow cytometry. We also thank Jianguang Ji at Lund University for statistical advice and David Brodin at BEA core facility for help with gene expression analysis.
Competing Interests: The authors have declared that no competing interests exist.
Funding: This work was funded by a grant from the Swedish Cancer Society, the Swedish Research Council, the Cancer Society in Stockholm, the Karolinska Institutet Funds, and the Stockholm Country Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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"The Kindness of Strangers - The Whittington Hospital"
About: The Whittington Hospital / General surgery The Whittington Hospital General surgery N19 5NF The Whittington Hospital / Gynaecology The Whittington Hospital Gynaecology N19 5NF
Posted by hawley (as ),
I went in for minor surgery (laparoscopy and hysteroscopy) in the Day Treatment Centre at the Whittington Hospital and was amazed by the care I received while there - the staff were wonderful and good humoured.
I also really appreciated the kindness and attentiveness of the nurse(s) in Recovery, the competence and clear communication of the anaesthetist and gynaecologist and their teams, the gentleness of the orderlies and the thorough cleaning that was going on around me.
Going to see a doctor - let alone being in hospital - has always been something I've dreaded. Like most people, I don't relish the idea of being vulnerable. In fact, my fear of dying has largely been born out of this dread.
But while I was in the care of the staff at the Whittington, I felt a weight lifting. The dread has disappeared, and while I hope that I'm not a patient again for some time, I don't mind now if I am.
My recovery has been easy - no doubt because I was treated so well.


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Care 4 Cars Inc
Review for Care 4 Cars Inc
Chuck And Marilou J. on 07/30/2015
Chuck said this business takes really good care of his vehicles. He is really close friends with the mechanics and he enjoys the employees.
Vehicle: Chevrolet Corvette
Category: Service
Service Date: 07/16/2015
Review Created: 07/30/2015
This review was collected via phone interview by Customer Research Inc.


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4 Moyle Street, Port Augusta, SA 5700
3 Bed | 1 Bath | 3 Car
Property ID: SA6839178
This lovely brick veneer home has a fantastic rental history and is currently leased at $225pw. There are 3 good sized bedrooms and the bathroom is rather exquisite, featuring a large bath and matching vanity unit. The lounge is of exceptional size, and has a large window that lets the afternoon sun in, great for colder months. A rear alfresco area is ideal for entertaining, and the yards are abundant with trees, shrubs and plants, and a great area to relax in. There is adequate carporting and shedding - ideal for car and boat storage - and the location is perfect as it is within walking distance to the hospital, primary school and the ever popular back beach area.
View CENTURY 21 Top of the Gulf (Port Augusta)
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Gregory Edwards
Mobile: 0427092503
Email: Email Me


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Buoyant U.S. industrial figures helped shore up sentiment in the markets, sending stocks higher Friday while the impact of the Bank of Japan's latest apparent intervention to weaken the yen proved short-lived.
In Europe, the FTSE 100 index of leading British shares closed up 0.9 percent at 5,598.48 while Germany's DAX rose 1.8 percent to 6,298.3. The CAC-40 in France was 1.9 percent higher at 3,782.48.
In the U.S., the Dow Jones industrial average was up 1.7 percent, at 10,843.53 soon in midday trading New York time, while the broader Standard & Poor's 500 index rose 1.9 percent, to 1,146.49.
European markets and Wall Street futures had been trading lower, but upbeat U.S. durables goods data prompted the recovery.
The Commerce Department said orders for durable goods excluding transportation orders jumped 2 percent in August, double the amount expected by economists. It was the biggest jump in orders in five months and helped soothe concerns about the pace of the U.S. economic recovery following a run of disappointing economic data.
Overall orders including transportation did fall 1.3 percent, but that number is often severely impacted by month-to-month swings in orders for new aircraft.
"The devil continues to be in the detail of this report, as large swings in commercial aircraft and defense orders cause the headline to consistently miss consensus expectations," said Michael Woolfolk, an analyst at Bank of New York Mellon. "While the headline number was a modest disappointment, everything else proved positive."
Earlier, the news that German business confidence unexpectedly rose in September, as measured by the Ifo Institute, was diluted by the fact that most businesses in Europe's biggest economy expect tougher conditions in the months ahead as the global economic recovery slows down.
Most activity in the markets Friday centered on an apparent intervention in the currency markets by the Bank of Japan to stem the export-sapping appreciation of the yen.
Indications that the central bank was back in the market buying dollars and selling yen pushed the U.S. currency up from around 84.50 yen to over 85 yen. However, by mid-afternoon London time, the dollar was down 0.1 percent at 84.32 yen.
The dollar has been in retreat since Tuesday when the U.S. Federal Reserve indicated that it was ready to announce fresh measures to boost the flagging U.S. economy.
The markets are now anticipating that the Fed will turn on the taps once again at its next rate-setting meeting in early November, and that the fresh supply of dollars could lead to further weakness in the currency despite the slowdown in economic growth implicit within Thursday's figures.
After falling Thursday following weak euro-zone data and renewed jitters about Ireland's debt problems following news that the country shrank a further 1.2 percent in the second quarter, the euro was up 1.2 percent Friday at $1.3474.
Earlier in Asia, Japan's Nikkei 225 stock average, which was closed Thursday, lost 1 percent to 9,471.67 as the strong yen pressured exporters.
Elsewhere, Hong Kong's Hang Seng index rose 0.3 percent to 22,119.43 while Australia's S&P/ASX 200 fell 0.7 percent to 4,601.90.
The turnaround in stocks boosted oil prices too - benchmark crude for November delivery was up $1.13 at $76.31 a barrel in electronic trading on the New York Mercantile Exchange.
Associated Press Writer Alex Kennedy in Singapore contributed to this report.


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Cuddyer expects to return on Sunday
Cuddyer expects to return on Sunday
DETROIT -- Michael Cuddyer had the stitches removed from his right index finger on Monday and said that he expects to be back on the field on Sunday, the day he's eligible to come off the 15-day disabled list. "If it's not right on that day, then somewhere around that day for sure," Cuddyer said. "I don't foresee this being an extra week or anything like that." Cuddyer dislocated his finger on April 4 when he slid headfirst into third base in a game against Kansas City. He collided awkwardly with Royals third baseman Alex Gordon on the play, and he also suffered a deep laceration on the finger from Gordon's spike.
The finger is still a little swollen, but Cuddyer is planning on trying to do some light throwing and hitting on Tuesday. After those sessions, he said, he'll likely have a better idea of exactly when he will be back in the lineup. "When I come back, it's not going to be normal like it was before I got hurt, as is the case with any injury," he said. "But it will be good enough to play."
Kelly Thesier is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.


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Come join Sonny Clark and Roger Noriega on the call of this Divisional Round of the 2012 IFL Playoffs live from the Allen Event Center in Allen, Texas.
Come catch Monday Night Football IFL style, as the # 3 Wichita Wild take on the #2 Allen Wranglers.
The Wild and Wranglers have played four times this season and have split them 2-2. The winnner moves on to the Conference Championship being held next week.
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- Please select a date to see the proper prices!
- Meal can be requested on the spot
Best offer
58 € / person / night, no meal
Description of the accommodation:
Rooms, services Du Château INTER-HOTEL Pontivy is offering 27 tasfefully furnished, nice rooms for those looking for a place to stay at in Pontivy. Parking is availably nearby for those arriving by car. We recommend trying some of the relaxing wellness services available at the hotel, an indoor pool will also be at your disposal. Car rental service is also available to help you discover all key highlights of the area. The café and the bar provide a nice atmosphere for a romantic evening talk or a personal meeting. Wireless Internet connection (WiFi) is available at the hotel, ensuring that you can stay up-to-date during your holiday. The exact equipment and the exact list of available services of Du Château INTER-HOTEL Pontivy can be read on the information sheet below.
Payment Credit card guarantee or advance bank card payment may be required for the reservation, depending on the chosen room, date and price.
Arrival, opening hours The earliest time to check in to your room is 18:00, the latest time to check out of your room is 11:00. The personnel of the hotel can speak in French and English with you.
Du Château INTER-HOTEL Pontivy rooms Rooms
- Please select a date to see the proper prices!
Accommodation services
Free WiFi in shared places
Car rental nearby
- Indoor pool
Bar, Cafe, Elevator


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Enter to win a VIP seat and a gift bag for you and one guest at our private fashion show!
- Like Le Thrift on Facebook.
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- Le Thrift will select a winner on October 16th. Winner will be notified via Instagram.
Contact us at email@example.com with any questions. Good Luck!


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First Tutors helps you to find a private business related tutor in New Quay and across the UK. Private business related tuition is an excellent way to improve confidence as well as improving grades and doesn't have to be dull! Please use our service to find New Quay business related tutors and select which best fits your needs.
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Rum & Bones
The age of piracy is at hand! Davy Jones is dead, his treasure scattered to the Seven Seas. And whosoever can seize it all, will claim the forbidden power of Davy Jones' Locker itself. Run out the guns, and prepare to board! Accept no quarter, for there's none to give!
Rum & Bones is a miniatures board game where players control fantastic pirate crews engaged in fierce boarding combat.
Inspired by MOBA style video games, Rum & Bones' gameplay is deep, fast-paced, and highly strategic. Each ship's singleminded crew of Dexkhands and Bosuns charge headlong across the gangplanks to attack key deck features on the enemy ship. But the crews are evenly matched, so it's up to the player controlled Heroes to shift the tide of battle!
Each faction has five types of Heroes at their disposal: Captain, Quartermaster, Swashbuckler, Gunner and Brute, each with a different role in the fight. Each Hero is unique, with their own set of attacks, abilities, and reactions to crush the enemy or give their crew the push they need!
The Wellsport Brotherhood and Bone Devils factions included in this box also have access to unique powers via their Tide Decks. Just beware that these startling abilities might just attract the attention of that mythical terror from the depths, the Kraken! Prepare to storm the seas with Rum & Bones!
Game Contents
Players: 2-6
Ages: 14+
Average Game Time: 1 hour
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Sales of rotisserie chicken are soaring, and deli and food-service executives interviewed by SN say that at least for the moment, the sky's the limit.
One reported sales up 25% to 30% in the last six months. Others had strong sales reports to crow about, although some added a note of caution that the popularity of roast chicken from the deli could be a fad.
With busy, health-conscious consumers plucking up the chickens as fast as they come off the rotisserie, retailers are wor


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VideoHive Cubic Transition 1204665
After Effects Project \ Video Displays \ Corporate
After Effects Project - Cubic Transition by LiyaLandDesigns @ VideoHive
After Effects Project \ Video Displays \ Corporate
After Effects Version: CS4, CS5; File Size: 57mb; Requires Plugins: No Resolution: 1280x720
This is my new project "Cubic Transition".
- CS4 , CS5 Project
- 12 placeholders for video/photo and 12 text phrases
- Resolution Hd(1280X720)
- No plugins needed (Optical Flares prerendered)
- Customization in a few clicks
- Video tutorial included
- Duration 1:18
Key Words: business, colour, corporate, family, flare, grunge, music video, photo, photo gallery, photographers, portfolio, slide, slideshow, stylish, wedding.
Download Link


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IBEW Union Members: Defining Retirement Goals
Need help figuring out your retirement goals?
- Help define your retirement goals and expectations
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If your contract offers a retirement plan from The Principal and you're eligible, login to access retirement plan information and planning tools. Click the following link for great tools that can help you determine what your expenses might be in retirement and how to plan accordingly.


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he can't visit ur page?
u won't receive any messages from him?
what exactly does this blocking function do?
he can't visit ur page?
u won't receive any messages from him?
what exactly does this blocking function do?
Hahahahaha ... my guess is that they (the angels) have not fixed it yet?
When you block someone, the fact is that they cant visit your page, but (yes, there are many "but" on this site, because the "angels" have not, and most likely cannot, done it correctly) there is a very simple way for him/her to see and comment your photos ... and oh, you will still receive message from him/her too, provided he/she has figured it out ...
In other words, the "blocking" is a lie ...
I dunno how it works for you but whenever I block someone they just create another anonymous account.
(continued) ... but that's about all "blocking" can do ...
By the way, "blocking" will not block those comments on the "bubble" (front page) ... in other words, as soon as anything about "you" appeared on the front page, the person(s) you have blocked can write/comment and you will see it in your profile ... and this is even NOT the "photo gallery backdoor" that I was talking about ...
(continued) ... I dont know, it's probably a new feature ... Kyle?
That dildo never goes away. Ever.
It's an indelible dildo.
Go on. Say it.
If I do, it's not because you said so. Deal with it. It was just there.
(continued) ... Rob, heehee, did you mean reach or teach?
If you meant "reach", then the answer is "no", if you meant "teach", then the answer is probably "yes" ...
Hahahahaha ... I dont know "why", but then, why should I care?
(continued) ... heehee ... do go on :)
(continued) ... heehee ... do let him/her go on, this site lacks entertainment ...
Hahahahaha ... again, do tell .. please ...
(continued) ... hahahaha ... so you decided that your friends are not worth your trouble?
What message? The show is over? Damn ... and I have rushed back from my lunch too ...
(continued) ... heehee ... because you have been talking about no one besides yours truly ...
I have better things to do than put you back in your place..
You have no idea how much they tried ...
(continued) ... heehee ... I will say the same ... since you have been assuming many things about me ... oh, I meant imagining ... no, I meant fantasizing ... oh, you know what I meant ...
(continued) ... heehee ... talking about judging people ... you have it really bad ...
I'm only basing my judgement on what I see from you, out in the open.
So, I have said or done something "in the open", care to help me refresh my memory, even? Or, again, you made that up?
On the other hand, I am guessing that I have asked one too many girl to block or dump you, so you are just here to let off some steam?


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Japan Society Winter Staff Picks: Recipes
With the changing of the seasons comes a time for nostalgia, festivities and celebration—what better way to celebrate these changes than with food! From baked oatmeal to hotpot recipes, finger foods to lunchbox ideas, Japan Society staff members share their favorite recipes to accompany the colder months.
Kabocha Eggplant CurryJapanese comfort food at its finest. Leftovers for breakfast with buttered toast remind me of chilly winter mornings with my whole family around. The kabocha and eggplant, particularly with chicken thigh, make a nice change from typical beef curry.
–Japan Society Staff MemberView the recipe here.
Vegetarian Sushi Rice PizzaI'm very fortunate to have a friend whose career as a Personal/Chef Assistant has exposed me to brilliant food recipes. She's such a good friend that she started experimenting with vegetarian recipes when she found out I stopped eating meat. The sushi rice vegetarian pizza that she made for me was one of my favorite food experiences. I am one happy guinea pig.
–Manuel Martinez, FinanceView the recipe here.
Chanko NabeThis delicious hot pot dish is one of my favorite Japanese meals for winter. I first had it for lunch while visiting Ryogoku, Tokyo, an area famous for both sumo and chanko nabe. It's eaten by sumo wrestlers to gain weight, but it's actually quite healthy with lots of vegetables and protein– just don't eat too much!
–Lydia Gulick, DevelopmentView the recipe here.
This dish is famous in Yamanashi Prefecture, and made only during the winter months. Throw everything you have in a big pot or nabe, add thick gooey noodles to the mix and you have a delightful dish that will warm you from the inside. In Japan where most homes are not centrally heated, this is the perfect way to spend time with family and friends–enjoy a regional dish and keep your family warm.
–Lana Kitcher, DevelopmentView the recipe here.
Chicken Teriyaki over RiceThis is of one my kids' favorite lunch boxes. It's easy to make whether for one person or for the entire family. This recipe is easy enough to make even in my very busy morning hours!
–Mie Igarashi, DevelopmentView the recipe here.
Grilled Chicken with Peanut Sauce over RiceEvery time there is a family gathering, my grandfather will buy hundreds of sate ayam for us to feast on. Sate ayam is an Indonesian dish of grilled skewered chicken served in peanut sauce. This recipe is my one–meal take on the dish; easy to reheat and prep in advance. Best eaten with fish crackers or lentil chips over rice.
–Leonie Thebez, DevelopmentView the recipe here.
Goya and Tuna SaladThis is a wonderful and easy dish for those who don't like to cook, want to use seasonal vegetables from Japan, and are hoping to get some healthy vegetables into their diet. Eat it plain or as a sandwich–it is a treat for all! (Be sure to soak the slices in salt beforehand. Goya is known to be very bitter!)
–Lana Kitcher, DevelopmentView the recipe here.
Goya ChanpuruGoya chanpuru is a distinctive dish from Okinawa, Japan's tropical islands. It's made with the bright green goya, an Okinawan gourd that is very healthy but can be bitter on its own. When mixed in to a stir fry like this, all the flavors meld together beautifully. I make this dish without pork for a vegetarian version and it's still delicious!
–Anna Cabasso, Talks+View the recipe here.
Tonjiru Soup (Miso Soup with Pork and Vegetables)Fall and winter are the perfect seasons to eat tonjiru soup. It's chock-full of healthy vegetables and pork, and it will warm you up from the inside out. I usually make a big pot of it for easy weekend meals. It's also my go-to dish when we have a small gathering of friends at our home. Typically it is made with a regular potato, but I like to use one regular potato and one sweet potato to balance out the flavors. For me, this dish conjures up memories of visiting a mountain temple in Nagano on a cold and snowy New Year's eve, and eating tonjiru afterwards at one of the many food vendors that line the streets.
–Maureen Kamata, Toyota Language CenterView the recipe here.
Finger Food: Miso-Sriracha Deviled EggsWhen I'm not planning events for Japan Society members, I enjoy entertaining at home. These two Asian-influenced dishes (miso-sriracha deviled eggs and matcha marshmallows) are always a hit among my Japan-centric circle of friends.
Nobody likes making deviled eggs, but everyone likes to eat them! This variation on the classic retro dish may be better than the original.
–Christy Jones, DevelopmentView the recipe here.
Finger Food: Matcha MarshmallowsAlthough making homemade marshmallows can be a little intimidating (and messy), the result will be fluffy, delicious and better than any store-bought confection! Serve them on their own or in hot chocolate. Note: this recipe requires a candy thermometer and a standing mixer.
–Christy Jones, Development
View the recipe here.
Matcha Mille CrepeThe matcha mille crepe is a hybrid style with French and Japanese elements. The smooth cream, bitter matcha, and soft crepe combined together perfectly creates its unique flavor. When I was an undergraduate student in Japan, I could find this type of cake in almost every convenience store in Tokyo. Since they were cheap and delicious, I bought them often as a snack when I got hungry.
–Zhewen Jiang, Business & PolicyView the recipe here.
Roasted Mango and Coconut Rice Pudding (Sombi)In 2012, Senegalese chef Pierre Thiam graciously took the challenge of creating the menu for Going Global!, a special international feast benefitting Japan Society's Education and Family Programs. He integrated Asian ingredients into ten Senegalese dishes, one of which was the memorable and extraordinarily delicious Sombi (Roasted Mango over Coconut Rice Pudding "Sushi"). Check out his beautifully illustrated cookbook, Yolele! Recipes from the Heart of Senegal.
–Michiko Grasso, DevelopmentView the recipe here.
Baked OatmealThis dish is a favorite around our home in the winter. At times, I will prepare the evening before and place in the fridge for a quick bake in the early morning with the aroma wafting through the apartment, it gets everyone up on those cold winter mornings. At other times, even our teenagers will make it and put their own twist on it with other fruits, nuts and occasionally chocolate or butterscotch chips. This is a crowd pleaser at all times and it's great for leftovers. My favorite way of eating it is hot from the oven with cold milk in a bowl!
–Jeffrey Miller, Education & Family
View the recipe here.
All illustrations © Giulia Venturelli/Japan Society; Chanko nabe photo © Lydia Gulick/Japan Society; Houtou photo © Yuichi Sakuraba; Teriyaki photo © Mie Igarashi/Japan Society; Goya Champuru © Hajime Nakano; Deviled eggs © Christy Jones/Japan Society; Matcha marshmallows © Christy Jones/Japan Society; Matcha mille © Ashley Lim; Roasted mango and coconut rice pudding photo reprinted with permission from "Yolele! Recipes from the Heart of Senegal" by Pierre Thiam, copyright 2008, Lake Isle Press, Inc.


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GraphicRiver Faith Begins Church Flyer Template 8210344
Photoshop \ Print Template \ Flyers \ Church
Church \ Options \ Marketing \ Includes \ Flyers \ Resolution \ Projects
Print Template - Faith Begins Church Flyer Template 8210344 by loswl @ GraphicRiver
Photoshop \ Print Template \ Flyers \ Church
The Faith Begins Church Flyer Template is sold exclusively on graphicriver, it can be used for your Church Events, Gospel Concert etc, or for any other marketing projects. The file includes 1 High Resolution Flyers with several color options for easy editing.
- One 8.5×11 Flyer
- Seven One-Click Color Options
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- CMYK/300 Dpi
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Fonts Used
- Helvetica
Layered: Yes; Graphics Files Included: Photoshop PSD; Minimum Adobe CS Version: CS2; Print Dimensions: 8.5x11.
The Shout Connection Card Template; Seven Messages Church Connection Card Template; The Promise Movie Poster Template; Fido Movie Poster Template; Born 2 Drive Movie Poster Template; Activate Movie Poster Template; No Other God Church Bulletin Template.
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Los Olvidados (The Forgotten Ones) (7/8)Uploaded by Wurdulak. – Classic TV and last night's shows, online. SYNOPSIS: The winner of two Cannes Film Festival awards, Luis Buñuel's Los Olvidados (aka The Forgotten Ones and The Young and the Damned) was the director's first international box-office success. Yet Buñuel showed no signs of curbing the outrageous… Continue Reading
CINE ALTERNATIVO Film Festival by OCCCA starts January 3rd
CINE ALTERNATIVO is a film festival produced by OCCCA, the Orange County Center for Contemporary Art: "Cine Alternativo is a film festival from Mexico, Central America, South America, North America, the Iberian Peninsula, Chicanadas and others Latinos theme. This offers a different perspective and a new look at the popular culture in the USA. We… Continue Reading
Antonin Artaud, A Love Triangle & Chilean Cinema in "DRAMA"
Congratulations to Chile's Matias Lira, who will be screening his directorial debut feature film "DRAMA" (Produced by Lira/ Written by Eliseo Altunaga, Sebastián Arrauin and Lira) in Park City at Slamdance 2011. Indiewire's Sydney Levine of "SydneysBuzz" helps cover the story HERE, but the LFF hopes to gather more information on this film and filmmaker… Continue Reading
ASALTO AL CINE/THE CINEMA HOLD-UP playing at Sundance 2011
Stay tuned for the first feature film by Mexican director Iria Gomez Concheiro, premiering at Sundance Film Festival 2011. The film continues to explore themes of rebellious youth and the "gritty boundary between adolescence and adulthood" that Gomez Concheiro touched on in her successful short film DIME LO QUE SIENTES/TELL ME WHAT YOU FEEL, an… Continue Reading


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Who do we reach? CIOs, ICT managers, procurement managers, IT infrastructure managers, telecoms managers, IT architects, security officers, and more… We focus on their priorities and needs, helping them to maximise the potential of digital technology.
Share the strength of over 1480 members (including 1010+ ICT decision makers).
For a complete overview of the topics we prioritise until September 2018, take a look here for our action plan.
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Complaint Review: Levitz Furniture Company
- Levitz Furniture Company
91 S. Martin I. King
Las Vegas, Nevada
- Phone: 800-445-8318
- Web:
- Category: Furniture & Furnishings
Levitz Furniture Company you won't love it at Levitz. They sold defective furniture and will not honor their merchandise RIPOFF Las Vegas Nevada
When the sectional came, it was damaged on the recliner piece, so I spent several days trying to call them to come out. Finally someone actually answered the phone and we had them out several weeks later to have the thing taken a part and fixed. We thought everything would be alright.
However, 4 months later, I noticed the cushions on this piece were too short in the back and on one side. So I spent several weeks this time trying to get through to there service department. I finally had to write a letter through there website. Meanwhile I visited their las vegas store to see if maybe the cushions were all made like this.
Wrong! The floor sample was not like what we had. What we had was a defective section on this couch, and I started calling at 7:am, on the nose to finally get someone on the phone. They made arrangements for someone to come out in Aug, 2005. The person said the cushions had slipped and open the back of the sectional and fixed the cushions, and then sprayed a sticky spray and assurred me it would now stay and not slip anymore.
I was happy. Who would have thought it had two cushions on each seat. Doesn't look like it, but oh well as long as it was fixed. WRONG! Less then two weeks later it started slipping again and once again I had a hard time trying to get a hold of them to come out. And when I did finally make arrangements again, for Sept, 24th, they never showed up.
So I called again several weeks later. Oct 8th, a no show again, no calls no shows. I finally wrote a very stern letter through there website and the person Claude Vera. They made arrangements for Nov,5th. Oh someone did show up took a picture of the front of the furniture(which wasn't the problem. It is the cushions that is what he should have taken a pic of, where the back cushion meets with the bottom one.
He was rude. He tried to fix it the way the other person did, only he left it short and still crocket amd the material holding the back of the material on the frame is torn thanks to their service people. I tried to explain to him, it was not straight. He had not sprayed it with the sticky stuff either. He just ignored us, stapled it up in back, torn off a tag, would not speak to us nor answer our questions. I asked for a copy of what he was writing. So he gave it to me, and I made a copy, then gave it back to him and he left.
Well I wrote this Claude Vera a letter telling him what his service person acted like and did, on the 5th of Nov. Then waited well after several weeks. I wrote another one to this same person. Today I get an email from them stating and I quote "Thank you for your recent email to Levitz Furniture. According to the service report that we received your merchandise is up to standards.Therefore there is no further action that we are going to take on your account. Although we empathize with you, we regret being unable to resolve this matter to yor satisfaction.
Thank you for choosing Levitz. We appreciate your patronage, and value you as a customer.
Claude Vera
Executive assistant
Written Correspondence.
First off, no one fixed the problem. The cushions are slipping side ways and forward, and the only way to fix it, is to open the back up. I have had nothing but problems with this one piece of the sectional, from the day it arrived.
Now this company wants to blow it off. No I don't think so. I want a replacement for this part. I feel I bought a new sectioanl, not a defective one, and I am very upset with Levitz and how they just pass the buck.
So if anyone has some advice on how to get them to do the right thing,let me know. They shouldn't be allowed to get away with this crap.
You'll love it at levitz. No I don't think so. Ripped off by levitz is more like it.
Now I will file my Consumer Protection complaint,and other companies I should contact folks?
Las Vegas, Nevada
Click here to read other Rip Off Reports on Levitz
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This report was posted on Ripoff Report on 12/12/2005 09:13 PM and is a permanent record located here: http://www.ripoffreport.com/reports/levitz-furniture-company/las-vegas-nevada-89110/levitz-furniture-company-you-wont-love-it-at-levitz-they-sold-defective-furniture-and-wi-167728. The posting time indicated is Arizona local time. Arizona does not observe daylight savings so the post time may be Mountain or Pacific depending on the time of year. Ripoff Report has an exclusive license to this report. It may not be copied without the written permission of Ripoff Report. READ: Foreign websites steal our content
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Fruity hodgepodge
Les Gourmandises de Sophie
Delicious sweets to discover
Composition :
Sugar, corn syrup, gelling agent: gelatin, citric acid, natural and artificial flavors (white / pineapple, yellow / lemon and orange, orange / yellow, pink / cherry and strawberry, red / cherry, plum / purple) dyes. May have an adverse effect on activity and attention in children. Net Weight: 110g
With this product, we recommend :
110g Jar
5.80 €
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9.50 €
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80g Jar
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150g bag
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Combined with state government and external resources, Virginia's Gateway Region communities offer competitive incentive packages. The region has numerous competitive business advantages and a welcoming business environment, not to mention the Commonwealth of Virginia's unparalleled reputation for business success. For the extra financial boost in building a business case, VGR can develop an incentive proposal for you to take advantage of some of the following incentives for new and expanding businesses. Contact us today to take a closer look.
Tax Exemptions and Reductions
Property Taxes
- Virginia does not tax intangible property, manufacturers' inventory, furniture, fixtures, corporate aircraft or certified pollution control facilities and equipment.
- Localities can fully or partially exempt: certified recycling equipment, rehabilitated commercial/industrial real estate for up to 15 years, manufacturers' generating and cogenerating equipment, certified solar energy devices, and environmental restoration sites (eligible real estate in the Virginia Voluntary Remediation Program).
- Localities may elect to tax the following at reduced rates: research and development, tangible personal property, equipment used for biotechnology research, development and production, semiconductor manufacturing machinery and tools, computer hardware and peripherals, aircraft, clean-fuel vehicles, tangible personal property used in the provision of certain Internet services and energy-efficient buildings.
Sales and Use Tax Exemptions
- Manufacturers' purchases used directly in production, including machinery, tools, spare parts, industrial fuels and raw materials
- Items purchased for resale by distributors
- Certified pollution control equipment and facilities
- Custom computer software
- Utilities delivered through lines, pipes or mains
- Purchases used directly and exclusively in research and development
- Most film, video and audio production-related purchases
- Charges for Internet access, related communications services and sales of software via the Internet
- Purchases used directly and exclusively in activities performed in cooperation with the Virginia Commercial Space Flight Authority
- Semiconductor clean rooms or equipment and other tangible personal property used primarily in the integrated process of designing, developing, manufacturing or testing a semiconductor product
- Computer equipment purchased or leased for the processing, storage, retrieval or communication of data in certain circumstances
- Machinery, tools, equipment, and materials used by a licensed brewer in the production of beer and materials such as labels and boxes for use in packaging and shipment for sale
State Corporate Income Tax Credits
The Virginia corporate income tax rate is 6%, with no unitary tax levied on Virginia companies' worldwide profits. For companies with significant payroll and property in Virginia, there is great benefit from a double weighted sales factor in the state's income apportionment formula. Tax credits are available for the following:
- Major business facility jobs – $1,000 per job over a 25- to 50-job threshold
- Recycling equipment –10% of original capitalized cost
- Green job creation – $500 for each annual salary of $50,000 or more, up to 350 jobs
- Day care facility investment – Maximum of $25,000 for expenditures incurred to establish a day-care facility for the children of employees.
- Worker retraining – 30% of all expenditures for eligible worker retraining
- Research and development tax credit – 15% or 10% of the first $167,000 or 20% of the first$175,000 of research done in conjunction with a Virginia college or university
- Port volume increase – companies using Virginia port facilities that increase their cargo volume by at least 5% in a year are eligible for a $50/TEU credit
- International trade facility – $3,500 per new employee or 2% of the amount of capital investment made to facilitate the increased eligible trade or $6,000 per job or 4% of capital investment in a "tobacco-dependent locality"
- Barge and rail usage – $25/TEU moved by barge or rail instead of by truck, for international trade facility companies
Reduced Training/Recruitment Costs
The Virginia Jobs Investment Program offers custom recruiting and training services and direct funding to offset recruitment and training costs. This program is limited to for-profit companies with new full-time jobs paying entry-level wages of at least 1.35 times the federal minimum wage, but this requirement may be waived in areas with very high unemployment. A company qualifies for the programs below based on the outlined criteria.
- New Jobs Program: For companies creating at least 25 net new jobs within 12 months from the date of the first hire and making a new capital investment of at least $1,000,000 in the location/expansion.
- Workforce Retraining Jobs Program: For a for-profit manufacturing operation or distribution center retraining at least 10 full-time employees (or at least 5 if the company has fewer than 250 total employees), and making a new capital investment of at least $500,000 associated with the retraining project.
- Small Business New Jobs Program: For companies with 250 or fewer employees company-wide, creating at least five net new jobs within 12 months from the date of the first hire, and making a new capital investment of at least $100,000 associated with the start up or expansion.
Enterprise Zones
The Virginia Enterprise Zone Program targets special areas in communities throughout the state for business development and expansion. Base-level state incentives are available for companies expanding or locating in these zones (view zone maps here). Additional incentives are available on a community-specific basis.
- Job Creation Grants: Eligibility: Company must create at least four jobs that include health benefits and pay at least 175 percent of the Federal minimum wage. Personal service, retail, food and beverage positions are excluded. High unemployment areas have reduced wage requirements (150 percent of minimum wage) to qualify. Value: Up to $500 per qualifying job over the four-job threshold, per year, up to 5 years. For companies paying twice the minimum wage, up to $800 per job per year is available.
- Real Property Investment Grants: Eligibility: Company or individual must invest at least $100,000 in a rehabilitation/expansion project or $500,000 in new construction. Value: 20 percent of the excess above the minimum investment requirement. For companies investing up to $5 million, the maximum grant is $100,000. For companies investing more than $5 million, up to $200,000 is available.
- Click here to download VGR Community-Specific Enterprise Zone Incentives
Discretionary/Performance Incentives
Compensation value for the below programs varies by project according to investment and job creation. Performance agreements tie a company to its commitments, and requirements may be slightly altered depending on other project factors.
- Commonwealth Opportunity Fund: Deal-closing cash grant requires 100% match from the locality and threshold amounts (varying by locality) of qualifying investment and job creation.
- Tobacco Region Opportunity Fund: performance-based grants for projects that create at least $1 million investment and 10 jobs in Virginia's "Tobacco Region", including Dinwiddie and Sussex Counties in the Gateway Region, and do so within 360 months.
- Virginia Investment Partnership Grant: A performance incentive for existing Virginia companies making at least $25 million capital investment while maintaining stable employment.
- Major Eligible Employer Grant: Must invest at least $100 million and at least 1,000 new jobs.
- Virginia Economic Development Incentive Grant: Targeting headquarters investment with new jobs paying at least 50% over the local average wage and at least $6,500 per job.
- Clean Energy Manufacturing Incentive Grant: Eligible companies engage in manufacturing of equipment/products related to energy production, conservation, storage or efficiency, requires capital investment of at least $50 million and 200 full-time jobs paying at least the average wage. For wind energy manufacturers, the requirements are lower – a minimum of $10 million investment and 30 full-time jobs paying the average wage.
- Governor's Agriculture and Forestry Industries Development Fund (AFID): Targeted at new or expanded facilities producing value-added, Virginia-grown agricultural or forestal products.Grants up to $250,000 or 25% of qualified capital expenditures (whichever is less), vary based on project factors.Requires matching commitment from community applying for the grant on behalf of business beneficiary.
- Port of Virginia Economic & Infrastructure Development Program: For companies engaged in Port activities and located in close proximity to the Port, including all Gateway Region localities. Amount varies per job depending on the number of jobs created, with maximum total grant $500,000.
- Transportation Partnership Opportunity Fund: Assists with transportation features connected to the project. Must meet Governor's Opportunity Fund minimum criteria.
- Economic Development Access Program: Funding to provide adequate road access to a project location. Up to $500,000 in unmatched funds, with up to $150,000 additional funds if matched by other sources ($300,000 total additional).
- Rail Industrial Access Program:Funding for rail improvements related to a project, up to $300,000 in unmatched funds, with up to $150,000 additional funds if matched by the community ($300,000 total additional).
Incentives for Port-Related Investment
- Port of Virginia Development Zone: incentivizes locations and expansions involved in exporting or importing through the Port and creating at least 25 new jobs. The special incentive zone covers the Gateway Region.
- International Trade Facility Tax Credit: Company must increase by at least 10% the amount of cargo transported through a Virginia Port Authority facility and hire new employees or make a new capital investment to facilitate trade.
- Port Volume Increase Tax Credit: Company must be engaged in manufacturing goods or the distribution of manufactured goods, using port facilities in Virginia, and increase its port cargo volume at these facilities by a minimum of 5% in a single calendar year over its base year port cargo volume (requirement may be waived for major facility). The amount of the credit is generally equal to $50 for each TEU above the base year port cargo volume.
- Barge and Rail Usage Tax Credit: Available to international trade facilities, as defined above, that transport cargo through Virginia ports by barge or rail rather than by trucks or other motor vehicles. $25 per TEU or 16 tons of non-containerized cargo moved by barge or rail.
Foreign Trade Zones
A Foreign Trade Zone (FTZ) is land located at or near a U.S. Customs port-of-entry, where foreign and domestic merchandise is considered to be in international commerce (not in U.S. commerce territory). Foreign merchandise may enter and be exported from this area without formal customs entry or the payment of customs duties or government excise taxes. Such fees are only due at the time they leave the FTZ and enter U.S. customs territory. When foreign components are brought into the FTZ and combined into a new product, the user may elect to pay the lower of either the duty applicable to the product or to the component parts, and no duty is paid on the value added to the product by domestic parts or labor within the FTZ. In short, the FTZ offers an extremely flexible method of handling domestic and imported merchandise, allowing users to save time, transportation and handling charges.
Richmond International Airport is FTZ #207, and operates subzones in the region at Southpoint Business Park in Prince George County and just outside Chesterfield County at the Port of Richmond.
Port of Virginia is FTZ #20, and both Surry and Sussex Counties are eligible for the establishment of subzones within this territory.
Two other FTz are within 150 miles of the Gateway Region: Dulles International Airport, Washington, D.C. – FTZ #137 and Culpeper County – FTZ #185


text:
Development News & Reviews
More Development News
The terms "app testing," "analytics" and "mobile devices" have rarely been used in the same sentence—maybe never. This might be an actual first for the IT industry.
Quick Base research points to greater diversity among business app builders as compared to traditional programming jobs, with 40 percent identifying as women.
Jaikumar Vijayan | Updated
New Firebase feature will allow mobile app developers to better test different versions of their UI and application functions to determine the ones that perform the best.
Chris Preimesberger | Updated
Plan to join us Nov. 8 for an #eWEEKchat about the hot trend in do-it-yourself application development designed for non-technical line of business users.
The University of South Florida needed a way to improve processes, eliminate waste and enhance the user experience. Here's how Appian's low-code platform helped the university do just that.
Although rivals in the cloud, the technology giants find common ground in helping developers dive into the world of deep learning applications.
The new scholarship partnership with Udacity is part of a broader Grow with Google initiative that CEO Sundar Pichai announced this week.
Chinese company will open seven research labs: China (Beijing and Hangzhou), the U.S. (San Mateo, Calif. and Bellevue, Wash.), Russia (Moscow), Israel (Tel Aviv) and Singapore.
The new SaaS offering aims to make it easier for developers to build, package and deploy container applications to any cloud-native application architecture.
Despite ongoing discussions about the slow, tedious pace of growth in the U.S. economy, software's economic impact is surging, according to a new study by Software.org.
At the JavaOne Conference Oracle highlighted a number of new innovations in Java 9 and where Java 18.3 will be headed in the next six months.
Cloud Firestore, like the existing Realtime Database, is targeted at users of Google's Firebase app development platform.
The long, slow process of building new Java releases is getting a major overhaul in Java 9 as project Jigsaw finally lands.


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JB CYCLING ACADEMYgetting kids on bikes
Come along and take a ride with the Jacques Brink Cycling Academy Knysna
At the cycling academy in Knysna we love cycling and love sharing the joy of the wind in your face ! Discover the sights and smells of the forest. We want the next generation to share our love of cycling, so bring the family – there is nothing more rewarding to see that excitement and enthusiasm on their faces.
Superstore vs Quality MTB for your child
The temptation will always be there to grab a bargain , but you do really get what you pay for ! I know the big superstore bikes seem cheaper and bike store prices seem a bit daunting . But you may be surprised to know you can find an affordable , yet branded ,...
In addition to riding ,
at the Jacques Brink Cycling Academy we will teach your children the techniques for them to get as much out of their cycling experience as possible. We want them to learn how to approach obstacles with confidence
What's more
is that we also just let them be kids… riding through puddles ,breathing in fresh air exploring and have some great adventures. They will also learn how to appreciate and look after their bikes and gain maintenance skills that will keep them and their bike going and going . Send them along for the fun !
Contact us today to arrange
Calling Young Mountain Bikers
For all of you who have young mountain bikers in Knysna please join us on Friday 7 April at 3.30pm @ The Farm We the Jacques Brink Cycling Academy will be hosting an obstacle and skills clinic( ages approx 7 to 13 ) . Fun and getting stronger at the same time . Learn...read more
Knysna Cycle Works Rides and Clinics
We offer the following : 1.Jacques Brink Cycling Academy Friday 4pm for junior cyclists 2. Junior Training Clinics For Sprogs , Juniors and ladies wanting to get a bit fitter and skilled . Will have the occasional "bootcamps" – cycling and hiking...read more
Moonlight Mass in Knysna
We haven't a had Moonlight mass cycle for a while and I wasn't sure who was going to come. It was a Monday night with school the next day . I was pleasantly surprised by the great turn-out . The many children and their parents. Lights were fitted , helmets...read more


text: Miami - A former New Orleans police officer was sentenced to five years in prison on Thursday for helping cover up a deadly shooting in the days after Hurricane Katrina struck New Orleans in 2005.Ten New Orleans police officers have been convicted in a federal probe into a police shooting on September 4 2005 that killed two civilians and seriously injured four others on the city's Danziger Bridge, just after the hurricane.Robert Barrios was the last of five police officers being sentenced who was linked to the shooting and cooperated with investigators.Barrios was in a group of police officers who drove to the bridge after five other police officers had opened fire against the civilians.Admitted to lyingIn April 2010, Barrios admitted to federal prosecutors "that he agreed with other officers to obstruct justice during the investigations that followed the shooting", the Justice Department said in a statement.Barrios also admitted to lying in a formal police statement to help cover for his fellow officers.US District Judge Eldon Fallon imposed the stiffest sentence possible because Barrios was the last officer to cooperate with federal prosecutors and failed to provide any new information, The Times-Picayune reported.For his co-operation, Barrios was only charged with conspiracy, which carries a maximum five-year sentence, the newspaper said, quoting lead federal prosecutor Barbara Bernstein.A jury convicted five of the officers on August 5 over the incident for either being involved in the shooting or in the subsequent cover-up. Four of them were found guilty on 25 counts and could now face life imprisonment.Botched responseThose five officers are scheduled to be sentenced in district court on February 14.Hurricane Katrina smashed through the city's poorly maintained levees on August 29 2005, causing Gulf of Mexico waters to flood in. Eighty percent of the city was under water at the height of the storm, and thousands were stranded on rooftops.Reports of widespread looting and armed gangs roaming the city shifted the government's already botched response to the disaster from humanitarian aid to a military operation.


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Another fisticuffs situation today...UGH! I heard it but I didn't see it. I was under my blanket practicing morning prayers so I decided to stay with something positive and not turn over to watch the action. When I heard what I thought was a fist hitting a skull, it was difficult to focus yet I persisted. There was lots of grunting, extremely vulgar language and the sound of slapping. "The tank" woke up and began voicing encouragement to "hit him again" and "he's down, kick'im". I heard someone say "The guard is just standin' there watchin'." At some point over the loudspeaker came "Code Blue, Code Blue...seven south, upper B". That's us! Both tribesmen were taken to the hole and their bunks are now ready for new tenants. Welcome to my world...a world that would have once terrified me into a dark place now becomes a movie of sorts, where I am amazed at "man's inhumanity to man". No longer a small boy living in fear of the man who might beat me, I am a good man who is saddened by the world in which I find myself.
I think I have an addiction (tongue in cheek here!). This morning the commissary brought coffee to all who had the coins to order it! I was one of the fortunate. Haven't had a cuppa for 10 days...count 'em...TEN DAYS! After my first cup (made with the hottest tap water I can find at 6AM ...from the bathroom pipes!), I was amazingly content. After the second mug, my body was completely satisfied. Is coffee the REAL MEANING OF LIFE?? :) Of course not...but "the tank" seems lighthearted at the moment....that counts for a meaningful HOORAY!
Just saw an ad for HnR Block. Felt like I was kicked in the gut. I won't be helping taxpayers this year...or ever again. I'm an Enrolled Agent (EA), licensed by the Department of the Treasury. (Google that one...EA!) I have had forty years of experience representing clients before the IRS. When I am sentenced, that license will disappear! I'm aware of how much my self-esteem is connected to being a "professional" and it does hurt to lose that EA designation. I don't have the vocabulary to describe that pain that the HnR Block ad caused me. About twenty minutes later there was a similar ad for Jackson Hewitt. Tis the season. I'll be seeing many of these TV spots in the weeks to come. I hope I get used to them.
MAIL!! I love receiving cards and letters....wink, wink, nod, nod! Got two letters this afternoon...call me Happy Camper! :) I also received two books yet I am uncertain about the source of these gifts!! By the time they get to me, books have all traces of mailing and all reference to the sender destroyed. SO I will list the author and title and say THANK YOU right here! It's such a challenge to be stimulated intellectually in "the tank". Reading and writing gives me a chance to get into my head so MANY THANKS FOR ALL THE BOOKS!! Received: Lullaby Town by Robert Craig and Mirage by Clive Cussler....for now, if you're reading this, you know who you are...and I THANK YOU FOR THE GREAT GIFTS!! What I really miss is a lively conversation...oh well, must be part of the punishment!
One more 2014 sunrise tomorrow...I'll be there to watch it! AND HAPPY BIRTHDAY for anyone born on December 31. Call your parents and remind them of the tax deduction you game them...HAH!
Next post will be next year...2015...an 8 year...no idea what that means?!@# Anybody out there know??


text: The 700 series burner sequence controllers are housed in an ABS plastic enclosure and has 70mmx110mmx105mm (L*B*H) overall size. The enclosure has a base and plug in controller module for easy replacement at sites. The models 710 - PR - MA (equivalent to MA-81O controllers) sensing by LDR, 712 - PR - 24 - PI4 (equivalent to LOA - 21 & LOA - 24 controllers) are used for Oil Fired Burners. Similarly the following models are used for Gas Fired Burners: 722 - FR - P5, where the flame sensing is done by using flame rod, 732 - UV - P5, where flame sensing is by UV sensor.


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favorite hummus
1 piece of flat bread (spinch is the best)
1/2 red pepper
salad greens
any other veggies that cook well with peppers (onions, mushrooms, etc)
1 tablespoon olive oil
1. Core And Dice Red Peppers and other veggies being used
2. Stir Fry veggies with olive oil until skins of peppers look slightly chared let veggies cool
3. Spread Hummus over flat vegan bread
4. When veggies are cool, evenly spread the mixure over hummus, leaving 2 section at the end of flat vegan bread w/ just hummus (this is so the roll will stick).
5. Add Salad Greens
6. Tightly roll the wrap, use tooth picks if needed to keep it together
7. Refrigerate for approx 1 hr to let flavors mix, but not too long or else it will be soggy!
8. Cut into 4s and enjoy!


text:
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Discussion in '1911 Forums' started by lsbbigdog, Jul 29, 2011.
$2800 isn't that a little high for a used gun. I think someone here (lawdog) got quoted something a little cheaper for a new build. (Pro Compact). It is very nice no doubt about that though.
Isn't that GJ's CC that wouldn't run?
I believe so.
That must be why he states it had an issue with the slide stop.
This exact build would cost more than $2800 to duplicate now, trust me. Unless he was after the same exact build, one quote doesn't equal the next.
Nope, the SS is fixed, I'm just done with SA.
Glad to hear it . It's a great looking gun and one to be proud of!
I second that, its a beautiful gun
That is a super sexy weapon!!
Nice gun for sure
Hope you didn't take that the wrong way. I was just asking.
I understand, I was just saying is all. The price jumps on just the "Radical Carry Bevel" now is crazy, I think it went up $150.
When I priced out a Custom Carry with SACS earlier this year....it was just north of $2,800.
5" Custom Carry Operator .45acp
flush crowned
ball cuts
Harrison trigger
10-8 rear/tritium front
flush slide stop
30lpi FS and MSH
Armory Kote
Wow, that's a pretty plain build for the price. I know they raised their prices back in 2010, must have been more than I thought.
Yeah, honestly I was shocked. George was a few hundy cheaper for it, but I was like $2,600.....yeah, no thanks. Would be just as happy with another Brown or Baer for $400 less.
Crazy, I know my FFL's price for a base CC was $1400 at the time. I would think your specs should have been around $2200.
I wish I would have kept to the simple, entry level 1911's now, but, live and learn.
Base CC was $1,800 I think. I was hoping to keep it close to your as mentioned $2,200. That's where I could get a railed Kobra with my KC build specs or a Baer Ultimate Recon for. Above that, I would consider a Guncrafter American or a Pro, but that CC price was absurd.
The sad thing about the CC is you don't even get checkering/front strap treatment standard. I never understood that. It is definitely crazy on the price you got for the options.
I went stupid when I ordered mine, and I have a feeling I'll either be stuck with them or take a bigger loss than I want.
Yeah no kidding. No FS done at $1,800 is just silly. With the selection of Browns/Baers available for less, that doesn't make any sense at all, and once you get the FS done you are at $2k, and that whole "diminshing returns" thing sort of slaps you in the face....and that doesn't include any other adds.
Yep, I have a feeling I learn this. I knew it when I ordered if I ever thought about selling one it would be difficult. I'm just testing the waters with mine for now.


text:
617 Route 9W
Glenmont, NY 12077-3705
Sales: (518) 463-3141
2014 BMW 3 Series 328i xDrive in Glacier Silver Metallic vehicle highlights include, *CLEAN CARFAX*, *ONE OWNER*, and *AWD*. BMW Certified and AWD. There isn't a cleaner 2014 BMW 3 Series than this well-appointed gem. BMW Certified Pre-Owned means you not only get the reassurance of 1 year with unlimited miles from the expiration of the 4 year/50,000-mile (whichever comes first) BMW New Vehicle/SAV Limited Warranty, but also a multipoint inspection/reconditioning, 24/7 roadside assistance, trip-interruption services, and a vehicle history report. A deal like this on such a refined car does not come up for grabs very often, so you better act fast.
617 Route 9W
Glenmont, NY 12077-3705
(0 Miles)
Sales: (518) 463-3141
Year: 2014
Make: BMW
Model: 328xi
Price: $24,480
Mileage: 34,625
Engine: 2.0L 4-Cylinder DOHC 16V Turbocharged
Exterior Color: Glacier Silver Metallic
Interior Color: Black Dakota Leather
Fuel Type: Gasoline
Stock #: BU00864
City MPG: 22
Hwy MPG: 33
Featured Options
Cold Weather Package
Premium Package
17" Alloy Wheel A/S
Dark Burl Walnut wood trim
Highlight trim finishers pearl
Navigation system w/ touchpad
Included Packages
Cold Weather Package
Premium Package
Additional Options
17" Alloy Wheel A/S
Dark Burl Walnut wood trim
Highlight trim finishers pearl
Navigation system w/ touchpad
CO2 relevant vehicles
Sport leather steering wheel
Tire pressure monitor
Universal garage-door opener
Floor mats
Auto-dimming mirrors
Auto-dimming rearview mirror
Power Front Seats
Split fold-down-rear seat
Storage package
Rain sensor and auto headlight
Automatic climate control
Ambient Lighting
Additional 12-V power sockets
Hi-fi sound system
DVD Area coding, North America
BMW Assist eCall
BMW TeleServices
BMW Online and BMW Apps
Advanced RTTI
Remote Services
BMW Apps - iOS and Android
Enhanced B/T and Smartphone In
Instrument cluster
Oil Chg 10,000 mls/12 months
Daytime driving lights
Shipping package
Transport protection
STEPTRONIC automatic trans.