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Detoxification of wood hydrolysates with laccase and peroxidase from the white-rot fungus Trametes versicolor
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Fermentation of wood hydrolysates to desirable products, such as fuel ethanol, is made difficult by the presence of inhibitory compounds in the hydrolysates. Here we present a novel method to increase the fermentability of lignocellulosic hydrolysates: enzymatic detoxification. Besides the detoxification effect, treatment with purified enzymes provides a new way to identify inhibitors by assaying the effect of enzymatic attack on specific compounds in the hydrolysate. Laccase, a phenol oxidase, and lignin peroxidase purified from the ligninolytic basidiomycete fungus Trametes versicolor were studied using a lignocellulosic hydrolysate from willow pretreated with steam and SO2. Saccharomyces cerevisiae was employed for ethanolic fermentation of the hydrolysates. The results show more rapid consumption of glucose and increased ethanol productivity for samples treated with laccase. Treatment of the hydrolysate with lignin peroxidase also resulted in improved fermentability. Analyses by GC-MS indicated that the mechanism of laccase detoxification involves removal of monoaromatic phenolic compounds present in the hydrolysate. The results support the suggestion that phenolic compounds are important inhibitors of the fermentation process.
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The Edit | Style Inspiration: Wonderful Wicker Part 3
Thursday 13th July, 2017
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Edwards Lifesciences Corporation, a leader in the science of heart valves and hemodynamic monitoring, recently announced the U.S. and European launches of its Carpentier-Edwards PERIMOUNT Magna Mitral Ease valve, designed to enhance implantation in the mitral position.
The Magna Mitral Ease valve incorporates new features to facilitate access, placement and suturing during both conventional and minimally invasive heart valve surgeries, especially those using a thoracotomy. Its product enhancements, such as the tri-colored holder, suture guide line, saddle-shaped cuff and new accessories are all designed for easier implantation. Magna Mitral Ease is based on the clinically proven and durable Edwards PERIMOUNT mitral valve design, which has more than 250,000 patient years of experience worldwide. Patients with tissue valves such as Magna Mitral Ease can hopefully minimize the need for the lifelong prescription of blood thinners and its associated risks.
Dr. Malakh Shrestha, Hannover Medical School, provided a preliminary, single-center case presentation on Edwards' investigational, minimally invasive, rapid deployment Odyssey aortic valve platform. This initial series of aortic valve replacement (AVR) procedures at Hannover using the Odyssey platform demonstrated an approximately 50 percent reduction in bypass and cross-clamp times when compared to conventional, isolated aortic valve surgery. The Odyssey platform is currently being studied in Europe as part of the TRITON trial, and additional data are expected to be announced after enrollment and patient follow-up is complete.
Dr. Shrestha provides paid consulting services to Edwards Lifesciences for educational programs.


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As I have shared on my blog before, I love going to Zumba. I really love working out, but it is something I do not get the chance to do all the time. With a family of six, dad working full time as a youth pastor, and mom going to school full time, our time is limited. Luckily, I have found a Zumba class in town that meets twice a week in the evenings. I have been able to go the last month or so, and it has helped my anxiety and stress level recede tremendously, which I am thankful for.
Although I love going, I have noticed some attitudes with the other ladies in class about my presence. It is not that they have said anything about my weight before (at least, I would not know because half of them speak in Spanish), but it is the way they look at me. Many of the other "big" women in the class stand in the back corners, all in black, hoping that no one see's them. They love coming, and they are great dancers, but the majority of them are trying to hide.
If you know me at all, you know that is not how I operate. I am not a hider, and I have been on stage since the age of nine as a singer. I have always loved being the center of attention (sad but true), so being up front does not bother me at all. I can see the instructor better, see myself in the mirror and see if I am doing the moves correctly, and I just like up front better. It is just who I am, who I have always been.
The problem comes when other women in the class expect me to hide like the other women. They seem completely confused as to why I am confident in my body and willing to look like a fool no matter what I am doing. Both gyms I have attended were this way. There was just a general sense of them not really getting why I could care less about being in front of other people as a big girl. Big girls in movies are funny when they want to be up front (Big Amy in Pitch Perfect) but people are not really sure what to do with that type of person in real life. They are not sure how to respond, since most of our self confidence and body assurance tends to come from what we look like.
I pray that at some point, I can point out that my confidence does not come from myself. I cannot and do not do what I do of my own accord, because without the help of a Father who loves me no matter what size I am, I know that I would completely avoid the gym forever. When I go to Zumba and shake it, I am doing it to look good in front of everyone else there. I am going because I love to dance, I love feeling good, and I love to work out. No matter what size I am, those things are true. I thoroughly enjoy living outside their stereotypes, and being the woman that God created me to be: fearless, sure of myself because of whose I am, and am willing to look like an idiot because it is fun. I will continue to do these things whether or not I am 500 pounds or 100 pounds, because I am fearfully and wonderfully made. I know whose I am, and that's all that counts.


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The new Olympic Channel brings you news, highlights, exclusive behind the scenes, live events and original programming, 24 hours a day, 365 days per year.
The Session of the International Olympic Committee (IOC) meeting in Lima, Peru, elected Paris as host city of the Games of the XXXIII Olympiad 2024 and Los Angeles as the host city of the Games of the XXXIV Olympiad 2028. "Congratulations to Paris 2024 and Los Angeles 2028! This historic double allocation is a 'win-win-win' situation for the city of Paris, the city of Los Angeles and the IOC," said the IOC President, Thomas Bach.


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Welcome to WebmasterWorld Guest from 220.127.116.11
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votes: 15
I also use Kiwipages, NZpages - there are many specialised travel directories too, but it would be hard to list them all. The main test for me is whether a site: command shows listings and TPR. Then I check its backlinks as far as possible.
[edited by: bill at 4:41 am (utc) on Mar. 15, 2007]
[edit reason] see sticky [/edit]
Strangely not linked from Yahoo!Xtra.


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What would you do if you discover the love of your life is an hermaphrodite and she kept you in the dark.
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Obstacle Chronicles 7/9
Dynisha spent the night with us last night. He woke up in the early morning hours calling for her, but then went back to sleep on his own.
The first part of the day went smooth as silk. The Dynisha and Jamie played with minimal arguing. M arrived a little before noon.
The first real meltdown happened after 1pm. M was playing with Jamie's shape sorter box and Jamie tried to take it away from her. Dynisha intervened and made him give it back. He cried, "It's my shapes! It's MY shapes." He stomped his feet. He put his hands on his face. He went for the sorter again and M smacked his hand. He ran down the hallway crying, "Kalah hurt me!" He didn't want comfort. A few minutes late he calmed down and the shape sorter was forgotten by all.
A few minutes later M got the board to a game that has numbers on it. Jamie again tried to take it from her, "It's my numbers!!" This skirmish was very short lived because M wasn't really interested in the board. Moments later a new skirmish... This time Jamie got M's doll stroller and she tried to take it from him.
Everything was dumped out in the playroom. The disaster was unbelievable. I told the kids they better get the room cleaned up. When M put a pony into the changing table she and Jamie argued because they both wanted to shut the door. Nobody wanted to pick up. Jamie kept running and getting into my bed. I made him go back and help. Every few minutes there was some arguing. Usually because Jamie was trying to take toys away from M or Dynisha because he wanted to put them away himself. Despite this he kept yelling at M to "pick up". He pitched a fit when Dynisha put his yellow car in the car basket. He had a mini meltdown and kicked the floor a minute. He yelled at her a few times, "That's my yellow car!" Several times he told the girls, "Don't touch me!" When I investigated, they had touched his hand or something.
Jamie got irritated at M for making clicking noises with her tongue. He kept telling her, "Don't say that." I had to ask him what he was talking about because I didn't hear anything but silence. Of course, since he told her not to she had to double her efforts to make the noise, which aggravated him a lot. I told her to stop to restore peace.
Hours later the room is a worse mess than what it started. Dynisha says there's too many toys and we should just throw them away. I say, fine and get her a garbage bag. She starts filling the bag quickly. I tell her, "Throw Mr. Bear in there, too. He's laying in the floor." Mr. Bear is her lovey. She begins to cry. I said, "See, it was really easy to throw their toys away, but when you have to throw away just one of yours it's not so easy." I told her that cleaning up the room is just as fast as throwing toys away. You pick it up and put in in the bin or on the dresser instead of in the bag. There's no difficulty difference.
I might have gotten through to her, but Jamie was another story. My little lesson backfired and had an unexpected result. I went outside for a minute and came back in to M screaming. Jamie was throwing everything in the trash. I told him to stop and he just kept on doing it. I told him, "No" and it wasn't sinking in. I walked out to think for a minute and decided to best plan of action was to make them take a break and come talk to me. I tried to explain to Jamie about cleaning up. Then I went into the room and began directing them what to do. If I left for even a second things melted down again. With three very agitated kids, it was not going smoothly at all.
At the end of the day, the room is not clean. I gave up.
Other than that we just had our normal bathroom arguments. I don't know why he doesn't want to go. He has very good control. He'll hold it forever and a day.


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text: We have fitted our Junior 36 with a 3 section fold away boom equipped with Air Injected tips. We believe these tips give a more effective spray pattern than the Flood Jets we were using, and much less susceptible to wind drift. Each section can be turned on independently, giving a choice of a 2, 4, 6 or 8' spray width. The boom will retro fit to older JR 36 models.


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Redmond, WA (PressExposure) April 25, 2011 -- "We started with one project on iPhone for a bay area customer. It was an application for child education and iPhone was new at the time," said an engineering team member from Harbinger Systems, a leading provider of software engineering services to some of the world's best product companies. "Subsequently, we created 7 applications for the same company that spanned retail, finance, airline and automobile industries, and included technology usage like location awareness, usage of camera, synchronization, push notifications, web services and many more. We also helped the company build similar Android applications. It was a great experience to give wings to the company's vision in the mobile applications space. I would say that our ability to ramp up so fast on these technologies is why we come out with flying colors time and again," he concluded.
The customer in question above went on to refer Harbinger to another company; something that is considered a norm in this company. Harbinger has enjoyed 100% repeat business over many years and through changing technologies. The practice areas that Harbinger specializes in include eLearning, enterprise software, high-tech systems testing, mobile, web 2.0 and gaming.
With the technology industry changing very quickly in areas like mobile, gaming, social media and web 2.0, Harbinger bets on organizational learning and talent management. "One of the main reasons for our success is the continued learning attitude of our team," said Swati Ketkar, Executive Director of Harbinger Systems. "We have been in business for over 20 years, but if you look at our services, you will see that we provide software development services for iPhones and iPads, Android, Web 2.0 and many cutting edge technologies, in addition to ones that are well established."
Ketkar graduated from University of Michigan's Ross School of Business with an Executive MBA in 2010. She is a member of the international honor society, 'Beta Gamma Sigma' for academic excellence during MBA. In addition to being an example of 'the spirit of continuous learning' within Harbinger, the MBA would help her manage the rapid growth, she claims. Harbinger was recognized among the 50 fastest growing Indian companies and 500 fastest growing companies in Asia pacific by Deloitte in 2009.
It is an honor few companies have received. When asked about the key driving factors behind the rapid growth, Ketkar added, "In addition to continuous learning and having an innovative mindset, one of the key factors is building strong trust relationships with customers. We have engagements with our customers that are well into their second decade. So, not only do we have to be on top of the game and lead with the latest trends in the industry, we have to build a working relation that is durable in the face of these changes."
For more information about Harbinger Systems, visit http://www.harbinger-systems.com.


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Greens and Independents willing to talk to all parties
Members of the Green Party and Independent Alliance (IA) have said they are willing to talk to any of the main parties about their conditions for supporting a government.
Green Party leader Eamon Ryan said he believed "all parties should talk to each other", while the IA's Michael Fitzmaurice said he was interested in speaking to other Independents to discuss how they could be "constructive" in the new Dáil.
Meanwhile, new Anti Austerity Alliance /People Before Profit (AAA/PBP) TD Gino Kenny said his alliance would wait "until the dust settles" before they met.
Mr Ryan said he and his fellow Green TD Catherine Martin "think all parties should talk to each other to consider how a government might be formed".
He said there was a homeless crisis and problems in health, adding: "If you want to manage that, you have to be willing to maybe compromise a little bit and say 'okay lets see if we could get a government and a programme for government that will address those crises'."
He didn't rule out entering government, despite the party being wiped out in terms of Dáil seats after its coalition with Fianna Fáil.
However, he admitted it was unlikely to happen as the party only had two TDs.
Five of the six Independent Alliance TDs met in Leinster House last night. The meeting was also attended by Tipperary Independent Mattie McGrath, who is not part of the grouping but who took part in discussions about the possibility of forming a technical group to secure more speaking rights in the Dáil.
Roscommon-Galway TD Mr Fitzmaurice said he and his IA colleagues "want to make sure that Independents work constructively to make sure that a government can be formed, and we will be leading the way in my opinion".
AAA/PBP's Mr Kenny ruled out a deal with Fine Gael or Fianna Fáil.
Irish Independent


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EQUESTRIAN: Allen stars for Irish in medals bonanza
Irish riders collected a massive medal haul at the European Pony Championships in Jaskowo, Poland, over the weekend.
The showjumpers and eventers took team silver, 15-year-old Bertram Allen claimed individual jumping silver and eventer Jodie O'Keeffe won individual bronze.
The event organisation was chaotic, with the pure dressage delayed to rectify the poor going in the arena, and there was disappointment for the British who were awarded joint-bronze only to have their medals taken back when a scoring error was discovered.
In the showjumping team competition, however, the British pipped the Irish for gold by just a single fault while Holland took bronze.
Irish pathfinder was 2010 individual gold medallist Allen -- with the wonder-pony Acapella Z -- who kept a clean sheet first time out. And when Max O'Reilly-Hyland (Rock Dee Jay) had one time fault and both Michael Duffy (Kadia Mouche) and Emma O'Dwyer (Jacknell Street) had one fence down, the Irish were trailing by five faults at the halfway stage.
Clears from O'Reilly-Hyland and O'Dwyer second time out saw them threaten for the lead, but the British completed with eight on the board while Ireland's total was nine.
In yesterday's individual final the medals were decided in a three-way jump-off between Britain's Beth Vernon and Jessica Mendoza and Allen. Vernon and her mare Falaza pipped Allen for gold, leaving Mendoza with bronze while Ireland's Emma O'Dwyer finished fourth.
The eventers missed team gold by a smaller margin as Josh Pim, Lucy Latta, Ana O'Brien and Jodie O'Keeffe completed with a combined score of 157.8, just 0.1 behind the victorious French.
O'Keeffe clinched individual bronze with Castleview Rakish Milly.
Irish Independent


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24/7 Comfort Apparel Women's Shirred Front Dress, Size: Small, Raspberry
This lovely, knee-length dress by 24/7 Comfort Apparel features a figure enhancing shirred front topped with a feminine V-neck, giving that show-stopping look to any figure. Slight stretch in construction and capped sleeves finish this pull-on dress with a chic, comfortable fit for any event throughout the year. The variety of colors allow you to express your own sense of style. Size: S. Color: RASPBERRY. Gender: Female. Age Group: Adult.
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Pirates of the Caribbean: At World's End
PG-13 | 148 min | Friday, May 25, 2007
Movie Description
Captain Jack Sparrow and Captain Barbosa are back at it again in this third sequel of the Pirates series. The Pirate Lords fight and conspire to release the captive goddess Calypso from her trap. Full of superior swordsmanship and incredible battle scenes, At World's End is an exciting addition to the pirate collection.
Genre: Action Adventure Blu-ray Mystery
Disney Movie Rating:
82.06 out of 100. Total of 198 votes.
Pirates of the Caribbean: At World's End Trailer


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We were on the radio again yesterday. Here is some of what Marine Biologist, John Mussington had to say as reported today in the Daily Observer:
Over-fishing in Barbuda could affect economy
By By Tameika Malone – Monday, June 25th, 2012.
ST JOHN'S, Antigua – Marine Biologist John Mussington has warned that the over-fishing of the sister isle's marine resources could spell disastrous implications on Barbuda's economy.
Speaking on the Big Issues programme yesterday, Mussington maintained that uncontrolled exploitation of fish could increase its already costly food import bill. The marine biologist said something must be done immediately.
"We have to look at our food security. The average person in Barbuda depends a lot on having cheap, readily available fish for consumption and if this was to be lost, the impact would be more significant than on the average Antiguan.
The marine biologist said if this way of life was to be lost the implications would be immeasurable.
"When you consider because of the transport cost and other difficulties, everything in Barbuda is at least 30 per cent higher in price than in Antigua," Mussington said.
He pointed out that the local fishermen who have already wreaked havoc on Antigua's parrot or chub fish stock are now in Barbuda.
Mussington said while the fisheries there have done things differently than in Antigua, taking into consideration the fisheries resources, livelihoods and cultural lifestyles of its fisher folks, the problem is yet to be addressed.
"In Barbuda the export of lobster is very significant because it puts money into the pockets, directly and indirectly for a large portion of the population, so if anything is to go wrong with the fisheries, there is going to be more hardship on Barbuda's economy and by extension every single Barbudan," Mussington said.
However, the parrotfish is not the only marine species that this over-fishing would impact.
According to Mussington, the parrotfish plays an integral role in ensuring the existence of other species and these fish should be monitored.
"It's not just the chub fish that is going to be affected, the coral reef we know will be affected and that same coral reef is where we find our lobsters so there will be a domino effect," he added.
(More in today's Daily OBSERVER)


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VideoHive Rural Cycling 9095061
Stock Footage \ People
SunsetRiding \ Summer \ Sunset \ Sports \ Silhouette \ Children \ Holiday \ People
Stock Footage - Rural Cycling 9095061 by limette @ VideoHive
Stock Footage \ People
Riding a bike at sunset.
Alpha Channel: No; Looped Video: No; Frame Rate: 25; Resolution: 1920x1080; Video Encoding: H.264; File Size: 252mb; Number of Clips: 1; Total Clip(s) Length: 0:23.
Smartphone; City Map Guide; Billboard; Mobile Phone; Hands; Mobile Phone; Smart Phone.
Keywords: alone, bike, children, field, grass, holiday, kid, man, people, silhouette, sports, summer, sun, sunset.


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Story about lance lambert
My name is lance lambert, I'm male and 33 Years old. My Sign of Zodiac is leo. I live in Havana, Cuba. On Flirtic.com I've been registered for 14.11.2012 years.
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I speak english. My mother tongue is english.
lance lambert
Was online 19.08.2014
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As I predicted yesterday, the clergy and commentators defending Pope Benedict's response to the clergy sex abuse scandal — including a legal defense being planned in the U.S. — has fueled a backlash from victims and their supporters.
After the American bishops affirmed that they "stand with" the pope and the Vatican's handling of the pedophile priest problem, the Survivors Network of those Abused by Priests, aka SNAP, issued an outraged press release:
Instead of exposing predators and helping victims, America's bishops busy themselves with self-congratulations and public relations. They tout their plentiful but largely ineffective policies, panels and procedures which were adopted as public relations moves. Since 2002, they have done little but water down and increasingly ignore their pledges of greater openness, confident that 'the wave has crested' and they have little to fear from more victims, lawsuits, prosecutions or media exposes.
Once again, bishops still refuse to use the words "bishop" or "cover up" when talking about a crisis caused by bishops covering up. This statement, like so many others, tries to minimize this crisis, blaming only the predators and ignoring their corrupt supervisors.
SNAP also announced that a chapter of the Chicago-based group is forming in England, which should make Pope Benedict's September travel plans — the first official visit to Britain by a pope — more interesting.
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