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Unfortunately, this job is expired as of 1/2/2015.
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Riverside Community College District
Director of Basketball Operations/Head Basketball Coach
Application Deadline: 01/02/2015
Interested candidates must apply by the deadline listed below by 8:00 p.m., Pacific Standard Time.
Department: Athletics (R)
Position Type: Academic Management
College/Campus: Riverside
Physical location of the position: Riverside
Employment Type: Full-Time
Position Length: 12-month
Salary Range: Grade T
Salary Amount: $81,989 - $99,817
Salary Type: Annual
Is this position categorically (grant) funded? No
Under the direction of the Director, Athletics, the Director of Basketball Operations/ Head Basketball Coach is responsible for the development and maintenance of an effective and competitive CCCAA (M&W) Basketball Program. This position will also encompass assistant athletic director duties and serve as a lead administrator in the absence of the Athletic Director. The recruitment and retention of quality students in both men's and women's programs will be integral to this position. This position will also plan and coordinate the Annual RCC Holiday Basketball Tournament, sport community relations and work with different areas in the community while creating volunteer opportunities for the student-athletes. This director will oversee all aspects of recruiting within the sport programs. This includes planning and coordinating recruiting visits, coordinating the recruiting database and mailing system. This position will travel with the team and assist with all logistics as it relates to travel and coordinate team video needs. The Head Basketball Coach will also be expected to demonstrate the ability to promote the physical, intellectual and social growth of student-athletes. The Head Basketball Coach is responsible for directing all aspects of their assigned sport program.
This position will serve as direct supervisor for the head women's basketball coach.
1) Master's degree from an accredited institution in a discipline taught at the community college is required.
2) Master's in physical education, education with an emphasis in physical education, kinesiology, exercise science, physiology of exercise or adaptive physical education OR a California Community College Instructor Credential in the subject matter of physical education is also required.
One year of formal training, internship or leadership experience reasonably related to the administrative assignment. Five to seven years recruiting and coaching experience at the collegiate level is required.
Rules and guidelines set forth by the NCAA and CCCAA, the Orange Empire Conference, and Riverside City College policies and procedures.
Develop sports skills, strategies and philosophy; promote scholar-athlete welfare and provide appropriate mentoring to scholar-athletes and assistant coaches; effectively communicate within the department and with the College community on matters pertaining to the overall basketball program (Men and Women); maintain standards of professional conduct and ethics.
Basketball Operations
• Direct both men's and women's coaching staff with the flow of daily correspondence and day to day operations of running a sport program.
• Work with the Athletic Director to develop short- and long-range planning for overall student success initiative and success of RCC athletic program.
• Assist the Athletic Director with the overall fundraising/sponsorship initiatives, including the Annual RCC Holiday Basketball Tournament and directing staff in his/her absence.
• Make recommendations to Athletic Director to improve/change current policies/procedures as it relates to game operation and overall game environment.
• Responsible for developing a manual for recruiting, academics and athletes, etc.
• Responsible for coordinating the head coaches calendar in regards to recruiting and appearances in the community.
• Responsible for distributing compliance bulletins to staff for each recruiting cycle, informing compliance of coaches' travel schedule, maintaining and turning in phone logs to compliance, proactively identifying and resolving any walk-on eligibility issues, staying up-to-date on all new legislation and inform all staff, preview official and unofficial visit compliance paperwork.
• Prepare reports, records, and other related documents as it relates to matriculation, transfer, and retention.
• Assist with the monitoring of academic progress of student-athletes.
• Establish and maintain an environment conducive to the welfare and academic success of the student-athlete.
• Establish and maintain effective interactions with students, faculty, staff, administrators, alumni, and the public.
• Create, develop, and direct a summer RCC basketball camp initiative.
• Direct staff in order to develop a senior camp, youth camp, team camp, and the logistics of each event.
• Responsible for all recruiting aspects of summer camp, this includes: providing lists of prospects attending camp to coaches, separating prospects from general campers on day of camp, providing recorded times to coaches during camp.
• Identify, hire, train and evaluate assistant coaches, including volunteers; ensure that assistant coach/volunteers adhere to all College, California Community College Athletic Association, Orange Empire Conference, and NCAA guidelines.
Athletics: Head Men's Basketball Coach
• Serve as head coach, advisor, mentor and role model in order to foster the physical, intellectual, and social development of student-athletes.
• Actively engage in campus-wide activities and within the Student Services Division.
• Maximize the physical talent of the basketball team through excellence in coaching, teaching, and the promotion of teamwork.
• Follow all rules and guidelines set forth by the NCAA and CCCAA, the Orange Empire Conference, and Riverside City College policies and procedures.
• Plan, organize and administer all in-season practice sessions for men's basketball, and develop team playing schedule in coordination with the Director of Athletics and scheduling offices.
• Retain student-athletes at RCC within the men's basketball program.
• Supply information in the preparation of the media guides; be available to media for interviews; be available for speaking engagements.
• Maintain inventory of equipment, uniforms, and supplies.
• Carry out team travel according to established policies and procedures.
• Serve as a member of the Management Leadership Association.
• Maintain an understanding of current ideas, research and practices pertaining to the areas of responsibility for this position, through continued study and participation in professional organizations.
• Perform other related duties as may be assigned.
Candidate must have evidence of sensitivity to and understanding of the diverse academic, socioeconomic, cultural, disability and ethnic backgrounds of community college students (E.C. 87360a).
Faculty, students and staff.
Normal office environment.
This is an Academic Management position with twelve-month contracts. Paid vacation, 22 days annually; and paid accruable sick leave, 12 days annually. A pro-rata reduction will be made for employees working less than full-time and less than 12 months. The District provides a health and welfare benefit package for employees and legal qualifying dependents.
The work location and assignment within a job classification is determined by the District and may be subject to change.
All offers of employment will be contingent upon the availability of funds and approval by the Board of Trustees.
The Riverside Community College District is an equal opportunity employer and recognizes the need to provide reasonable accommodations to employees with disabilities. For more information, contact (951) 222-8039.
The Riverside Community College District complies with all federal and state rules and regulations and does not discriminate on the basis of ethnic group identification, national origin, religion, age, gender, gender identity, gender expression, race, color, ancestry, genetic information, sexual orientation, physical or mental disability, or any characteristic listed or defined in Section 11135 of the Government Code or any characteristic that is contained in the prohibition of hate crimes set forth in subdivision (1) of Section 422.6 of the California Penal Code, or any other status protected by law. This holds true for all District employment opportunities. Inquiries regarding compliance and/or grievance procedures may be directed to the District's Title IX Officer/Section 504/ADA Coordinator. Harassment of any employee/student with regard to ethnic group identification, national origin, religion, age, gender, gender identity, gender expression, race, color, ancestry, genetic information, sexual orientation, physical or mental disability, or any characteristic listed or defined in Section 11135 of the Government Code or any characteristic that is contained in the prohibition of hate crimes set forth in subdivision (1) of Section 422.6 of the California Penal Code, or any other status protected by law, is strictly prohibited. The Title IX Officer, Section 504/ADA Coordinator for the District is Ms. Chani Beeman, 450 E. Alessandro Blvd., Riverside, CA 92508. Telephone Number is: (951) 222-8039.
Effective Employment Date: Mutually agreed-upon start date.
To apply, visit: http://apptrkr.com/548863
|Employment Type:
|Administrative Staff
|Degree Required:
|At least 1 years
|Level of Job:
|Not Specified
|Type of School:
|2 - Year Institution / Technical
|Application Requirements:
|Best if Apply By
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Artist description:
Lol XD I love chibis :3 The boy is my oc Mikuo who isn't Mikuo Hatsune okay? I don't own Mikuo Hatsune and I never made him...My oc is just named after him^^
Flash Draw Created Jan 15, 2010 338 x 470px Unless noted Copyright 2010 NARUTO1.
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sooooooo cuteee ^^ 5/5
sooo kyoooooooootttttttt
omg soooo cuuuute :'D
haha thats a cute chibi :3 and the bg is nice too. haha, havent been stalkerinn your draws for a while :P
OMG. Yay. Cute, simple draw~ Me lovies~ X3 Mikuo is soo adorable.
awwwwwwwwwwww he's cute :3! 5/5
awwwwz so cute!! x3 I might enter the chibi comp! ^^ 5h
so cute! awesome bg :D

subset: commoncrawl


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Freebee Friday: Making Visitors Welcome
Track those who can get into your school with PlascoTrac's Visitor Management Software. Aimed at K-through-12 institutions, the system allows staff to more efficiently and quickly track who is on campus as well as classify them by reason for visit. Inappropriate visitors can be flagged and photos are taken of each visitor for an ID badge. The system costs $600, but there's a free trial.


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Are scientists in the UK lagging behind their European counterparts in taking advantage of the opportunities to study abroad and learn another language? Mr. John Reilly, director of the UK Socrates-Erasmus programme, has warned that students in the United Kingdom will be unable to compete with their European peers unless more is done to promote the benefits of time spent studying abroad. In 1999/2000 about 100,000 students throughout the European Union participated in Erasmus, of which only 10,000 were from the UK. Universities blame student poverty, the perceived need to get into the job market quickly, as well as a lack of language skills as primary reasons for the apathy toward such schemes--and scientists seem the most apathetic of all.
Socrates-Erasmus is an exchange scheme between European universities, which allows undergraduate students (usually in their 2nd or 3rd year) as well as postgraduates to study at a university in another European country as part of their degree. Under the scheme, the normal student awards operate and, in addition, the EU pays an extra grant toward travel and living expenses. Recent figures show a decline in the numbers of UK undergraduate students taking part in the Erasmus programme, and of those taking part in the scheme, the majority are from language (32%) or business studies (20%) backgrounds with relatively few from the medical (2.7%) or natural (4%) sciences. In 1998/1999 only 387 postgraduate students participated in Socrates/Erasmus, although 10% of those were natural scientists. There is also wide variation between individual institutions in the numbers of students who get involved.
It is unclear exactly why so few science students take part, but it may be more difficult at the undergraduate level to find a course that provides an appropriate reciprocal year of study. Lack of language skills may be another reason. The Nuffield Languages Inquiry, an independent inquiry funded by the Nuffield Foundation, recently published a number of recommendations that it would like to see implemented in order to improve the UK's capability in languages. It puts forward a number of proposals for improving the language curriculum in schools, but it also suggests that a language should be a requirement for university entry, with specific courses tailored for those 16- to 19-year-olds that do not wish to specialise in languages.
All good news for the future if implemented, but not particularly helpful to the current crop of linguistically challenged British scientists, who appear to eschew the allure of European science and head straight across the big pond to the United States. Maggie Harnett of Glasgow University's immunology department believes that there has long been a perception that the best science is carried out in the U.S. or UK. That, coupled with the language barrier, has traditionally deterred British scientists from working in Europe. However, Harnett thinks that some European labs are gaining better reputations and that there may be a shift away from the U.S. as first choice for foreign study. But it seems that most British scientists still don't see any great need to improve their language skills as English is used in many European labs and at all international meetings.
This is not a sentiment shared by Guy Ourisson, president of the Academie des Sciences and Strasbourg University. At a recent European Science Foundation Assembly, he suggested that "teaching should be done in three languages at university level and in research." He claimed that a vital component of pan-European collaboration was greater linguistic versatility, posing the rather controversial question, "Is the universal use of elementary English in science really the only solution?" At the moment it would appear so, though greater efforts could be made by British scientists, if only to narrow the cultural gaps and make discourse with our European counterparts a more productive experience.
Despite the poor numbers of undergraduate scientists heading for Europe, there are significant numbers of Ph.D. students and postdocs taking up the challenge of working abroad. Steven Reid, an immunologist from Glasgow, spent 18 months working in Milan for an academic lab within a pharmaceutical company. Unable to speak a word of Italian, he signed up for a crash course at the Hetherington Language Centre at Glasgow University. Although this proved helpful, he still felt as if he was struggling for the first 6 months. He explains: "Communication in the lab and at meetings was O.K. as English was spoken most of the time, but at coffee and lunch breaks it was difficult to follow the conversation." Luckily, there was another British person in the lab, which made him feel less isolated. His employer also arranged for a private tutor to teach him Italian twice a week, resulting in fluency by the time he headed home.
Steven feels that the challenge of learning a new language and experiencing another culture was ultimately rewarding, and one which could enhance his career prospects. Increasingly employers are looking beyond their immediate regional labour market to a wider European market. Many European students are spending part of their higher education in another European country and will be future competitors in the ever-widening job market. For those who are interested in a work placement in Europe, as opposed to study, help is available through the Leonardo programme, which offers support for work placements and exchanges in science and technology. An exchange under Leonardo is an exchange of experience or know-how between firms, universities, training organisations, instructors, or lecturers. These exchanges generally last between 2 and 12 weeks, but can be longer.
If you are interested in working in a European lab but are worried about lack of linguistic ability, most university language departments have language laboratories and self-teach facilities, so it is worth contacting them to find out what is available. And many universities run extramural adult education classes in languages, which is a good way to meet nonscientists and brush up your language skills at the same time.


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So, I decided that the name New England Rippers reminds me too much of some sort of murderous gang. I wish to be granted permission to exit; however, if you decide to change the name to "Guys that live in New England that are united peacefully to share waves, that don't discriminate on craft style, that understand to be a master of the waves you must actually surf in winter" I will choose to stay, and even upload pictures with a watermark.
k thanks
Results 21 to 30 of 31
Thread: Group For Northeast Surfers!!!!
Feb 28, 2014, 12:26 AM #21Senior Member
- Join Date
- Jul 2011
- Posts
- 337
Feb 28, 2014, 12:51 AM #22
To me "New England Rippers" actually sounds more like an exotic dancers' union. Either way, I'm in.
Feb 28, 2014, 01:06 AM #23
Can we get a banner for this NE surfing group? Every group needs a banner. Where's jackrichard's banner salesman of a third uncle?
Feb 28, 2014, 02:42 AM #24
- Join Date
- Aug 2010
- Location
- Posts
- 3,540
- Images
- 1
Ya seriously I would like to fly a 'Belmar Nj-best waves in the NE' banner... Where is that guy???
Feb 28, 2014, 03:42 AM #25
OMG!!! Another Belmar joke! It gets funnier every single god damn time!!!!! So friggen funny!!! I can't wait to read 1,000,000 more BELMAR jokes!.
Feb 28, 2014, 04:17 AM #26
Bi-stater, it's like what you do with psychopaths, you ignore them and they end up going away. Not that everyone flying the BelMar banner is a psychopath.
Grommet, are you gonna answer the question of what now? You corralled us to this secret spot. All the brahs are here. And then...
Feb 28, 2014, 01:22 PM #27Senior Member
- Join Date
- Aug 2012
- Location
- Turtle Island
- Posts
- 12,783
- Images
- 6
Feb 28, 2014, 01:29 PM #28
NE Rippers reminds me too much of Full House. Worst band ever = Jesse and the Rippers.
Feb 28, 2014, 03:16 PM #29
Feb 28, 2014, 03:17 PM #30
Or like this:


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I know next to nothing about director Daniel Chan's upcoming gangster flick "Triad". That having been said, I've heard this sort of crime-oriented story a few dozens times before. A young guy works his way up through the ranks of a crime syndicate, decides it's time to quit, cleans up his life, and BAM! — everything comes crashing down. This is a gross generalization on my part, especially considering all I have to work with at this point is the nifty poster you see to the left and the generic synopsis embedded below.
And, like magic, here it is:
College-educated Harvard decides to become a gangster. He is promoted through the ranks, then heads his own team. As Hong Kong prepares for its 1997 handover, Harvard works with the police to ensure survival of his gang. His former boss BJ helps him out and they swear friendship. Harvard is able to move along with the times, cashing in on trends just ahead of the authorities. He gets into businesses beyond their jurisdiction, particularly internet porn.
Harvard goes legitimate, getting rid of the violent aspects of gangsterhood, alienating the fighters and those who cannot adapt. Harvard decides to resolve a gangland power struggle by himself – but discovers that the person betraying him is…
What? You didn't think they were going to tell you who betrayed him, did you? For a second, I thought they might. Gee, I certainly hope it isn't the best friend. Doesn't really matter what happens — I'll watch it regardless. "Triad", which stars William Chan, Michelle Wai, Patrick Tam, Irene Wan, Derek Tsang, and Edward Tsui, opens in China later this year.
Via : Film Smash


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KTM Roadbikes at Jennings GP track day
I was in Jennings Fl yesterday at a motorcycle track riding my Ducati. Noticed a group near me that had bicycles. Very common to see scooters and bicycles to get around the paddock. Went over to check them out because they were all on stands like on display. They were 3 beautiful KTM roadbikes. There were 3 or 4 guys from Miami and they are the Eastern distributors for KTM. Never knew they made bikes. They looked really nice and Mack answered any questions we had. He said the frames are made in Austria and they had Shimano components. Anyone know much about them?
They probably didn't make them but if they did, they'd be overpriced and impossible to work on... But fast as hell!
Pshaw. I've ridden KTM motocross and cx motorcycles for years. They're fine to ride and work on. AFAIK, they are a legitimate bike brand (unlike BMW etc.), but very likely like most brands have the bikes build in Asia.
I would bet that they are Taiwanese frames. Probably similar to Nashbar or Performance carbon frames.
Frames are sourced from Asia, but designed in house, and completely assembled in house. First started making bike frames in the 60's, but the full bike line is a relatively new endeavor for them. Just the last few years, if I remember correctly. They've been very successful in other markets, with their bikes being used by National teams. Several 10 star reviews, by Euro & Asian market testers...
I'm a little biased, as I have a 690 Duke & previous 950SM. :thumb:
I have to admit, when i saw a KTM frame over in the UK I was excited -- not enough to hunt one down, though. Somewhere I read they weren't the same KTM, but that may just be my imagining.
(As a current RC8R and former 950SM owner, as well)
|All times are GMT -6. The time now is 07:51 PM.


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Every family has secrets, but some are more dangerous than others...
Rebecca Desjardin isn't exactly looking forward to meeting Brandon's parents. The wealthy Hanovers won't approve of Rebecca's blue-collar P.I. lifestyle - and they certainly wouldn't approve if they knew she was a cat shifter.
Rebecca's Felis senses go on high alert when Michael, Brandon's father, strong-arms her into taking a quick job for one of his employees. If she doesn't agree, Michael will dig into Rebecca's past to prove she's not fit for his son.
When Rebecca discovers a dead body, she knows Brandon's father is hiding something. And she must decide if the truth is worth risking Brandon's love.
©2013 Sheryl Nantus (P)2013 Audible, Inc.
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Carmel Restaurant Reviews
Welcome to our Carmel restaurant reviews guide where you'll find top restaurants off the beaten path, both locally owned and chain. In fact, some of the best restaurants in the Carmel just might be the little mom and pop operations flying under the radar. For some of the best picks in Carmel, you've come to the right place.
Our Carmel restaurant reviews guide can help you make sense of the thousands of options out there. Happy eating!
Also check out our Best Restaurants in Carmel 2016 Guide
Displaying 1 - 20 of 30
Donatos Pizzeria - 106th & Michigan(04/02/2013)
"I work here and we have a mouse problem. I often find fecal matter throughout the restaurant. The manager encourages changing the dates on foods to save money and throws away pizzas that are perfectly edible instead of giving them to the firefighters right down the block. Overpriced, corporately own... "
Baskin Robins Thirty-One Merchant Square(01/26/2013)
"have gotten my daughter's birthday cake at BR for a couple decades - always the same - mint chocolate chip. Nothing that remarkable but she likes it. I understand the price increases. Everyone is facing the same pressures and I'm ok with coughing up over $30 for a special occasion. BUT THEY CHARG... "
Zacky's Hot Dogs(04/15/2011)
"Awesome food. Zacky and family get to know you. It's a very fun experience."
Glass Chimney(12/12/2010)
"Love, love, love this place! The bar special is $14.00 which includes a glass of wine or draft beer, and is a huge meal. They have a $30.00 special in the main dining room with a soup or salad, entree, and dessert! The food is quality ingredients and prepared perfectly. I grew up going to this e... "
China Garden(08/17/2010)
"Excellent food. The new owners are much better at their craft than the previous owners (Lin's garden). Great portions also."
Cancun Mexican Restaurant(02/05/2010)
"Food was excellent. Service was fast. I will definitely go there again"
China Garden(01/26/2010)
"A good Chinese take-out place. Ignore the review of Lin' Gardens from 2002, this is a new owner. The food is as good as you get around here. Eat-in motif is a bit sparse."
Mandarin House Chinese Cuisine(09/27/2009)
"What a pleasant surprise to stop in at Mandarin House and get such wonderful food. It will now be one our regulars. I'm only sorry it took us so long to find it."
La Hacienda Mexican(03/01/2009)
"Friendly staff, good well-priced food. Not particularly exciting but a solid, consistent experience. Try the Enchiladas Verdes or the Chicken Quesadilla."
"enjoyed time with friend; meal was huge for the money and delicious. Majors also offers Free Kids Meal with each adult and Free Facepainting and Balloons on Mondays 5:00 - 9:00 p.m."
Mitchell's Fish Market(07/16/2008)
"We went to Mitchells and were extremely disapointed. When I opened my silverware, the forks had old food crusted to it. The server's name was J**** and she was the worst server I have ever had in my life. The food was cold and it all came out at the same time (appetizers, salad, dinner). I would not... "
Mangia Italian Restaurant(04/26/2008)
"I have been here twice now. Food is excellent and always made fresh. They use the best ingredients. Service is great. Wonderful relaxing setting. Never crowded. Quiet and relaxing. I consider myself a discerning diner who has been around. Don't miss this gem."
Mandarin House Chinese Cuisine(03/29/2008)
"This is the best Chinese restaurant in Hamilton County. The food is excellent, the service is superb, the wait staff is friendly, and the value and portions are right on. My kids love the food and the lack of MSG makes your dining experience all the more better. I would recommend them to anyone wh... "
Mitchell's Fish Market(01/27/2008)
"Expensive for average food marketed as "fresh". One would hope so. Took forever to obtain a cocktail. French fries were lukewarm. Server was excellent when he finally showed up. This restaurant seems to be exploiting the expensive fish trend. I had a super cod fish fry for $12.95 at another pla... "
Abuelo's Mexican Food Embassy(01/23/2008)
"food is very bland. better than alot of the mom and pop mexican restaurants. but at about three time the price... not worth it."
Beijing City CO(01/06/2008)
"Excellent food, very clean dining area and kitchen. The employees are always friendly and love kids and show it. We eat in or carry out from Beijing once a week and have never been disappointed. Highly recommended for food quality and value."
Mitchell's Fish Market(12/28/2007)
"We love Mitchell's. The food, servers, bar, etc is just perfect. A great place to eat!!!"
Baskin Robins Thirty-One Merchant Square(12/13/2007)
"Very friendly staff, and excellent service. They know what I am going to get everytime and I just love that they care so much about the customer. I love getting my cakes from this Baskin Robbins because they make it look exactly how I want it."
Abuelo's Mexican Food Embassy(12/07/2007)
"This place is great and by far the best mexican food I have had in years. I really enjoy the chips they are so light and crispy and then the salsa was so fresh. Dont show up there without an appetite or you will be taking a ton of food home with you."
Cancun Mexican Restaurant(06/14/2007)
"We have gone to this restaurant since it opened and always enjoyed the food. Service excellant as was the food. However, for the first time I used a coupon. It was $6 off an order of $25 or more. The very tiny print stated that it cannot be used on specials. So I saved a dollar on the fish tacos but... "


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Jim Gaffigan: Mr. Universe
Once again Jim Gaffigan leads us down the road of guilty pleasures in this live smorgasbord of laughter. Selections from the menu include answers to such questions as why are whales so fat, even though they swim all the time? Do hotel rooms really bring out our worst behavior? And who is eating all that fast food? One of the country's most popular stand-up comedians, this laid-back guy next door is no stranger to your TV, including My Boys, That '70s Show, films such as It's Kind Of A Funny Story and many more. In his latest comedy special, Mr. Universe, Jim proves that he's in top comedy shape and he'll lead your whole family in a killer laugh work out!
- Length
- Rating NR
- Released:
- Added Jul 16 2012
- Production Year 2012
- Empire SKU 1621520
- UPC Code 014381827620
- Studio Image Ent.
- Packaging Keep
- Number of Discs 1
- Disc SS-DL
Features Not Specified
- Audio
English Dolby Digital Stereo
- Video
Widescreen 1.78:1 Color (Anamorphic)
- Jay Chapman


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For 10 years now, Wayne Lumgair has made a successful career for himself by being a wrench in the motocross industry. With tremendous knowledge and years of experience, he helped Rattray win the MX2 World Championship in 2008. Now, the successful duo is making their presence known here in the United States. We caught up with the Monster Energy/Pro Circuit/Kawasaki mechanic, and this is what he had to say...
What was the whole experience like coming from South Africa to the United States? Was the transition hard?
When I first left South Africa, I spent eight years in Europe competing at the Gran Prix as a mechanic. After I started getting some experience doing that, I started to work on some engines, too. Once I came over to America, though, that transition was a lot easier than Europe because South Africa is a lot similar to America. So, it's has been pretty easy because of that.
A lot of people always say that the riders from Europe are fast, but there have been a few top riders that have come out of South Africa, too. Is the motocross scene pretty big down there?
It's crazy how small the motocross scene is down in South Africa. If you saw it, you wouldn't even believe that you could get some good riders from there. For instance, at a National, you'll be lucky if you have like 12 guys on the starting line. And that's at a National! You'll probably get about 12 riders in the 250 class, and maybe about 10 in the 450 class. It's pretty crazy for sure. It's pretty crazy how they can produce such good riders because of the size. I don't really get it. Literally, the tracks are so beat up, and they are so small. There's not a lot of anything over there.
So, do a lot of the riders from South Africa head up to Europe to race the Grand Prix over there?
Umm, a lot of them try. First, Greg Albertyn set the record by becoming successful over there. After him, Grant Langston had some success, then Tyla Rattray, and now guys are trying to somewhat follow in their footsteps. So, it almost seems like it's a lot easier for South African riders to go up to Europe first than to come straight over to America. A lot of the riders do try, but not everyone is able to make it.
After you spent some time over in Europe, you finally made the move to the States. Has that been hard being so far away from your family and friends?
Well, when I first went to Europe, that was when it was really hard for me because that was the first time that I had been away from my family. So, when I came over to America from Europe, I was kind of already used to being away from home. So, it wasn't that bad, I guess. Plus, when I first came over here, everyone spoke English, and that made it really easy for me. The weather over here is also like South Africa, so there are definitely a lot of similarities between the two.
How has it been working with Tyla?
It's cool. I don't know what to say. He's really easy to work with, that's for sure. When we go out, he just gets on the bike and rides, and he doesn't complain a lot. He's not too fussy about things. As long as everything is straight and works, he's pretty much happy.
Now, did you guys live pretty close to each other in South Africa?
Actually, we lived together in South Africa. When we went up to Europe, we lived together for two years, but as he got older, he got his own place and I got mine. Everything just moved on from there.
For you, that has probably made your job a lot easier, wouldn't you think? I mean, you've probably got to know him really well over the years by living with him, and you probably know what it takes to get him motivated to win.
Yeah, pretty much because I was right with him from the time he left South Africa to now. I think he was 14 or 15 then. Whatever it was, I've been pretty much involved with him in some shape or form. Whether it was working on his bike, or whatever else, I was there. So, I pretty much know what works for him, and what doesn't work for him. I can pick up on how he is feeling, and how he's going to do. So, it's pretty cool.
How has it been wrenching for such an elite team like Pro Circuit?
It's really cool, and I enjoy it a lot. You're proud to be here, and you feel like you can't do things any better because they have the best. After my job, it's all up to the rider. So, it's quite a bit of everything.
How long have you been wrenching for now?
Well, I did it for eight years in Europe, and I've done it for two years over here. So, I've been a mechanic for 10 years altogether.
Has it been a pretty good experience?
I love it, and it's my life. I love the industry, I love the racing, and I just, I love it.


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50 Years of Smoking Warnings
On Friday, Acting Surgeon General, Boris Lushniak, released the 32nd report. Data in the document points to nearly 21-million premature deaths caused by smoking and exposure to secondhand smoke since that first report. The causes include cancers, heart disease, pregnancy-related issues and residential fires. Even in the face of such staggering numbers, Mary Gillett, tobacco prevention coordinator for Guilford Co. Dept. of Health. says there have been dramatic changes since 1964.
"People, both youth and adults, are very much more aware of the dangers of tobacco," she says, "although I find that most people are not completely aware of all of the dangers. There's a host of cancers, not only lung cancers, associated with smoking, heart and cardiovascular disease, and sudden infant death syndrome, so I think the awareness is broadening. The second thing is there are half the number of adult and youth smokers than there used to be, but there's still a tremendous amount of work to do."
Gillette says the rate of youth smoking is holding steady, although its at the lowest rate ever in North Carolina. The tobacco prevention program in Guilford Co. is funded by the Centers for Disease Control and Prevention, and has been going for more than 20 years.
One of the smoking cessation programs is Quitline , which Gillette says is available 24/7 in both English and Spanish. "You can sign up for a forward call program, and when you do that, it's really going to more than double your chance of quitting," she says.


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Update: Thursday, 9:45 a.m.
Goldens Bridge firefighters were forced to respond to their own house Monday morning, battling flames that severely damaged the firehouse.
Firefighters from at least eight departments responded to the blaze, which started around 9:20 a.m. on Monday, Feb. 24, and tore through the building for more than an hour. The Lewisboro Volunteer Ambulance Corps took one Goldens Bridge firefighter to Northern Westchester Hospital due to smoke inhalation, but no one was injured in the fire.
"It was incredibly scary," said Eloise Goldman, who lives behind the firehouse on Bradys Farm Road and called 911 after seeing the flames.
Route 138 was closed for the day as volunteers had to fetch more water at hydrants on Fairmount Road and at the corner of Todd Road and Increase Miller Road to douse the fire, which started on the first floor and rapidly spread to the second floor, leaving a gaping hole in the roof.
The flames were brought under control by firefighters from Goldens Bridge, South Salem, Vista, Croton Falls, Bedford Hills, Katonah, Somers, and Brewster. The Westchester County Cause and Origin Team and the state police determined Monday afternoon that the fire "was accidental in nature. At this time, the fire is believed to have originated from a faulty electrical connection to a fire truck while parked inside of the firehouse."
Goldens Bridge fire commissioner Ed Brancati and firefighter Mike Melillo were the only people inside the building when the blaze started.
Mr. Brancati said he was upstairs when he smelled smoke around a heating vent, and that the two men then checked the boiler room, which was clear.
"When I turned back and looked down the length of the building, two-thirds of the way down there was a wall of smoke," he said.
Mr. Brancati said that Mr. Melillo threw on his gear and rushed to get the department's vehicles out of the firehouse, but that a rescue truck and two Chevy Suburbans were damaged. One Suburban was destroyed, he said, while the other Suburban and the rescue truck were towed away to have the damage assessed. Mr. Melillo was able to get four trucks safely out of the house.
Mr. Brancati said he was not sure if the cause of the fire was something that could have been prevented.
"I have no idea because I don't know the nature of it yet," he said. "I'm sure at some point we'll have a little more clarity as to what happened."
Ms. Goldman said she was leaving her house when her sister-in-law, Robin Kern of Katonah, pulled into the driveway and asked if she saw the smoke coming out of the firehouse.
"About five minutes prior I heard the alarm at the firehouse," Ms. Goldman said. "Obviously I didn't think it had to do with the firehouse, per se. She [Ms. Kern] said to me, 'Turn around, do you see the smoke coming out of the firehouse?' And there was this terrible black smoke. I quickly called 911, and I said 'you're not going to believe this but the Goldens Bridge firehouse is on fire.'"
"We watched the whole building, we saw the fire start on the first floor, and then the whole building was engulfed on that whole side," Ms. Kern said. "Smoke was just pouring out of the building. It's just so bizarre that the firehouse would catch on fire."
"This fire was a testimony to how fast a fire can grow," said Goldens Bridge Fire Department Chief Albert Melillo. "They were alerted to the fire by a hard-wired alarm system that sounded. As well the rest of the membership was notified through a central alarm company, which notified our county-operated dispatch center. Edward Brancati, a district member, ran around the building, found the fire and alerted Mike Melillo that the engine room was on fire. Ed continued to search the building for any personnel as Mike ran into the engine room and was able to get four trucks out to safety before being overcome with smoke.
"I would like to thank all for their support during our time of need. If not for the efforts of some brave firefighters there could have much more damage or perhaps worse."
Next steps
Town Supervisor Peter Parsons said that officials were moving quickly to make temporary accommodations for the department and the residents that it serves.
Fire departments in South Salem, Katonah, Croton Falls, and Somers will help cover Goldens Bridge, he said, in addition to Goldens Bridge, which will have a temporary structure at its site on Route 138.
"The immediate concern is to ensure coverage right now," Mr. Parsons said, emphasizing that community safety is the town's top priority. "I am very impressed with the way the Goldens Bridge department planned the immediate coverage, is right into the short-term things that need doing, and I think that's pretty damn good."
Offices for the Goldens Bridge Fire Department will likely move into space at the Goldens Bridge Community House on Old Bedford Road. Lewisboro's Parks and Recreation Department currently occupies that building, but is scheduled to move to Onatru Farm Park in Lewisboro hamlet. Mr. Parsons said it could be a year or so before Goldens Bridge returns to a permanent firehouse.


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Perham FD eyes $250,000 fire truck
An upgrade from a 1968 to a 2003--that's what the Perham Volunteer Fire Department wants to accomplish with a new, quarter-million dollar fire truck.
Delivering the pitch for a new rig at the April 29 city council meeting were Fire Chief Mark Schmidt and officer Jon Ebeling.
Not only did the firefighters show a "power point" presentation in their sales pitch--they also rolled the rig into the parking lot, and invited city council members to come out, examine the truck, and "kick the tires." The rig was driven to Perham all the way from South Dakota.
Even though the department has focused in on the 2003 rig as its favorite, the law requires that the truck must go out for competitive bids. The unit was a demonstration unit for the Crimson fire equipment company in South Dakota.
Advertising for bids was approved by the council at the meeting. Bids will be reviewed by the council at the June 8 meeting.
Combined with the new water tower that will be constructed this year, providing the city a boost in firefighting water supply, the truck may help the department move up from a five "ISO" rating to four. The better the rating, the better the insurance premium rates to Perham businesses and residents.
The estimated value of the truck is $250,000. It is equiped with a 1,000 gallon tank, and has pumping capacity of 1,500 gallons per minute. The six person cab of the truck is also attractive to the Perham department.
If the truck were ordered today, it would cost up to $410,000, according to the Perham firefighters. Chief Schmidt noted that Detroit Lakes firefighters recently spent $408,000 on a rig; and Fergus Falls $350,000.
The 41-year-old truck presently in the fleet is worth an estimated $5,000. The tank leaks and it is a manual transmission. The 1968 rig has a 500 gallon tank, with a pumping capacity of 1,000 gallons per minute. To top it off, the unit hasn't passed a truck certification in two years.
"Engine number six needs to be replaced; we just can't put it off any more," said Chief Schmidt at the council meeting.
The $250,000 truck includes the base price of $220,000, plus specialized equipment requested by the Perham department at about $30,000.
The rig comes with a number of options included in the price, including a deck water gun, ladder rack, tire chains, a generator and other specialized equipment totalling an estimated value of nearly $60,000.
The truck will also improve firefighting capabilities for much of the county, said Schmidt, as Perham is one of 17 departments in the area operating on a "mutual aid" arrangement.
Firefighters ran on 106 calls in 2008
It has been a busy year for the Perham Volunteer Fire Department--with firefighters answering more than 100 calls plus assisting with flood duty.
A review of 2008 was presented to the Perham City Council April 29.
Firefighters ran on a total of 106 calls in 2008. Already in 2009, there have been 34 calls.
Perhaps the most intense fire of last year was the blaze that destroyed a mobile home and four vehicles in Richville.
That fire occurred late in the year.
The Perham department trained extensively, and many departments in the area also participated in the drills. Drills in 2008 included:
---A dramatic, Columbine-style school shooting drill--which involved nearly every emergency worker or volunteer in the area.
---A simulated school bus crash, near the Perham elementary and middle schools.
---A seminar titled "The Art of Smoke Reading," which attracted firefighters from throughout the region.
---Three separate house burn drills.
Perham Fire Chief Mark Schmidt also presented an outline of grants awarded over the past several years.
In 2007, the department received $18,500 from the Federal Emergency Management Agency, which was used to purchase a thermal imager device.
In 2008, $111,800 in FEMA money went to an air compressor and other equipment.
For 2009, Perham will be part of a county-wide grant of $1.2 million to upgrade the radio system. Departments also will contribute to the project, and Perham's share will be $19,000.


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Conservative pundit Ann Coulter sat down with "Fox and Friends" Sunday morning and gave her own scathing take on Newt Gingrich's big win over Mitt Romney in the South Carolina primary.
Coulter let it rip from the top of the segment, leading off with "Apparently, South Carolinians would rather have the emotional satisfaction of a snotty remark toward the president than to beat Obama in the fall."
She didn't stop there. Coulter continued on to make the case that Gingrich is the "least conservative, least electable" candidate who draws his support from the desire of the right wing to hear Obama get called a socialist.
Mediaite transcribed Coulter's comments as follows:
"This is not going to be the electorate in the fall. I am pretty sure we'll get everyone who voted for John McCain, since no one voted for John McCain, because we liked McCain, it was to stop Obama. We have those voters and now you have to get people who voted for Obama and having a candidate who calls Obama a Kenyan colonialist or socialist, that is not what you need, and at the same time, with Newt Gingrich you get the name calling for the president, very popular with the tea party crowd, not so popular with independents, and, he won't put a fence on the border but wants amnesty for illegals and took 1.6 million from Freddie Mac and he attacked Paul Ryan's plan on Social Security and with Newt Gingrich you throw out the baby and keep the bath water!"
Below is the clip of Coulter's fiery comments, courtesy of Fox and Friends:
(H/T: mediaite)


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Husky has recently come out with a 45-piece set of little truncated tools, lovingly named the Stubby Set. They were nice enough to send us one and it didn't take long before we realized that, although it's small and has a humiliating name, it's a really great collection of useful tools.
The contents of the Stubby Set include a ratcheting screwdriver, a dual-drive ratchet (able to accept 1/4" sockets on one side and 3/8" sockets on the other) and an adjustable wrench. For accessories and bits, the Stubby Set comes with:
The tools are all solid in the hands and the wrench has almost no wiggle to the lower jaw (a good thing). The head of the ratchet pivots 90 degrees each direction, giving the tool the ability to fit just about anywhere, particularly those tight spaces under the hood of your car.
But these tools aren't just for motorheads. It would be a great set for around the house and also would be valuable as a compact set for any tradesman. Perfect for those of you who run into bolts from time to time, but not often enough to justify lugging around a larger 100+ piece set.
And the best part of it all is that the Stubby Set only costs $15. Yep, that's right. 15 bucks for the entire set. It might be a little early to start thinking about Father's Day, but…
The Stubby Set comes with a carrying case and is available only at Home Depot.
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I bought a similar set also with a wide range of sizes, only to find out the reason they have such a range is they don't include every size, they skip every other size... for the price I guess that is not a huge deal until I realized that they skipped 1/2" what good is a starter type kit without a half inch? I think they need to re-think which sizes are left out.
just bought that stubby set a few weeks ago (minus of course the angle ratchet head) for 10 bucks from home depot and it has been perfect for small jobs. compact and varied its a good buy for the price for sure.
I went to home depot yesterday and they sell it now but the socket wrench doesnt angle like the one you reviewed. that was a big part of me wanting that.
It doesn't surprise that what is advertised isn't really there. The internet is full of crap, and for-profit companies are responsible for that. I just trust the store in my neighborhood as in the old times. Vendors on the internet are just too much heat, very little light.
We did some research on the Stubby Set and it appears that it was a limited time offer for Father's Day and that it is indeed starting to sell out in some stores. There is word that it may be back later in the year. We'll keep you posted.
--Tool Snob
home depot does not know of any huskey stubby $15 set .also 1-888-434-8759 (huskey tools )said the same when i called them .Is this the mystery item of the year ??????????????????


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Cameras used to detect speeding in Pennsylvania are not likely legal unless used by the Pennsylvania State Police. Even then, problems abound.
By Attorney Elisabeth K.H. Pasqualini, Traffic Attorney, Harrisburg, PA
I recently had a former client provide me with an article from an Ohio newspaper. The article discussed how the use of a video camera to detect speeders at an intersection was not lawful in Ohio. The Judge in Elmwood, Ohio called it "a scam that motorists can't win." The legal argument that undermines the use of such devices is that the operator (a robot) cannot be cross-examined. The device merely detects an object moving through the intersection at a higher rate of speed than is permitted and issues a ticket based upon the license number. This is a violation of the Confrontation clause of the Constitution, which permits an accused to confront or cross-examine his accuser. Without proper cross-examination, one cannot accurately defend one's case or test the veracity of the accuser's statements.
In Commonwealth v. De Pasquale, 509 Pa. 183, 501 A.2d 626, 1985 Pa. LEXIS 440 (Pa. 1985), the PA Supreme Court ruled that an ESP device (excessive speed preventer) was not permitted to be used by township police. This is because it is a mechanical or an electronic device statutorily only permitted to be used by a Commonwealth police officer (PA State Police or "PSP"). However, not all types of electronic devices are prohibited to be used by non-PSP officials. The relevant section of the statute states as follows:
- (2) Except as otherwise provided in paragraph (3), electronic devices such as radio-microwave devices (commonly referred to as electronic speed meters or radar) may be used only by members of the Pennsylvania State Police.
- (3) Electronic devices which calculate speed by measuring elapsed time between measured road surface points by using two sensors and devices which measure and calculate the average speed of a vehicle between any two points may be used by any police officer.
Not only would there be concerns that the device used in PA on a traffic camera was not permitted to be used by town, city, borough or township police officials, but a similar problem would likewise arise with the Confrontation Clause. If you've been cited for speeding and you think the method they used to determine your speed was inaccurate or inappropriate, contact Shaffer & Engle Law Office, LLC toll free or email us today.


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Previous studies demonstrated a requirement for multifunctional Ca2+/calmodulin-dependent protein kinase II (CaMKII) in PDGF-stimulated vascular smooth muscle (VSM) cell migration. In the present study, molecular approaches were used specifically to assess the role of the predominant CaMKII isoform (δ2 or δC) on VSM cell migration. Kinase-negative (K43A) and constitutively active (T287D) mutant forms of CaMKIIδ2 were expressed using recombinant adenoviruses. CaMKII activities were evaluated in vitro by using a peptide substrate and in intact cells by assessing the phosphorylation of overexpressed phospholamban on Thr17, a CaMKII-selective phosphorylation site. Expression of kinase-negative CaMKIIδ2 inhibited substrate phosphorylation both in vitro and in the intact cell, indicating a dominant-negative function with respect to exogenous substrate. However, overexpression of the kinase-negative mutant failed to inhibit endogenous CaMKIIδ2 autophosphorylation on Thr287 after activation of cells with ionomycin, and in fact, these subunits served as a substrate for the endogenous kinase. Constitutively active CaMKIIδ2 phosphorylated substrate in vitro without added Ca2+/calmodulin and in the intact cell without added Ca2+-dependent stimuli, but it inhibited autophosphorylation of endogenous CaMKIIδ2 on Thr287. Basal and PDGF-stimulated cell migration was significantly enhanced in cells expressing kinase-negative CaMKIIδ2, an effect opposite that of KN-93, a chemical inhibitor of CaMKII activation. Expression of the constitutively active CaMKIIδ2 mutant inhibited PDGF-stimulated cell migration. These studies point to a role for the CaMKIIδ2 isoform in regulating VSM cell migration. An inclusive interpretation of results using both pharmacological and molecular approaches raises the hypothesis that CaMKIIδ2 autophosphorylation may play an important role in PDGF-stimulated VSM cell migration.
- calcium/calmodulin-dependent protein kinase II
- cell migration
- adenovirus; autophosphorylation; chemotaxis; platelet-derived growth factor
vascular smooth muscle (VSM) cells in the medial walls of blood vessels are normally quiescent and express a differentiated phenotype that serves to generate and maintain vascular tone (18). However, vascular injury may result in dedifferentiation, migration across the internal elastic lamina, and proliferation of VSM cells to form a neointima (18, 33). Although the overall characterization of VSM within the neointima is well documented, the molecular mechanisms and signaling pathways that control VSM cell migration are not well understood.
One regulatory protein implicated in VSM cell migration is Ca2+/calmodulin-dependent protein kinase II (CaMKII) (13, 15, 35). CaMKII is a multifunctional serine/threonine protein kinase with numerous alternatively spliced isoforms arising from four genes (α, β, γ, and δ; see Refs. 10, 29). The isoforms have distinct tissue expression patterns, with the α- and β-isoforms located almost exclusively in brain tissue, whereas the δ- and γ-isoforms are found in both brain and peripheral tissues (10, 29). When free Ca2+ rises within the cell, Ca2+/calmodulin complexes bind to a conserved regulatory domain in CaMKII subunits, resulting in activation and substrate phosphorylation, including rapid autophosphorylation on Thr287 (Thr286 in the α-isoform) in the regulatory domain (9, 19). This autophosphorylation dramatically increases the binding affinity for calmodulin (16, 23) and renders the autoinhibitory domain inactive, providing the kinase with 50–70% autonomous activity in the absence of Ca2+/calmodulin (9). Functionally, this provides the kinase with the capacity to respond to Ca2+ transients in a frequency-dependent manner (6, 8). In addition, phosphorylation of this residue has been shown to be important in targeting the enzyme (26), facilitating the interaction of CaMKII and its substrates (34).
CaMKII is expressed in vivo as a large holoenzyme composed of up to 12 individual kinase subunits (10). Formation of the holoenzyme is dependent on a conserved COOH-terminal association domain (10) and has been shown to be important for autophosphorylation that results from an intersubunit, intraholoenzyme reaction (4). Because the holoenzyme structure can be heteromultimeric (29), a number of investigators are beginning to study how subunit composition affects holoenzyme targeting (2, 5, 27, 30). For example, a short, variable domain conserved in a number of subunits was demonstrated to contain a nuclear localization sequence capable of targeting CaMKII holoenzymes to the nucleus (25). In addition, the β-isoform of CaMKII has been shown to contain domains that provide targeting to the cytoskeleton and F-actin (21, 30). Such findings emphasize the importance of defining the CaMKII isoforms expressed in a specific cell type and considering structural features that may result in distinct protein-protein interactions and unique function.
Previous studies using cultured VSM cells implicate a regulatory role for CaMKII in PDGF-stimulated chemotactic cell migration (13, 15). These studies relied on chemical inhibition of CaMKII (with KN-62 or KN-93) or molecular manipulation of CaMKII activity by expression of a constitutively active CaMKIIα, an isoform not expressed in VSM (29, 35). We (20) previously reported that the δ gene is the main isoform expressed in cultured VSM, with the δ2 splice variant (also called δc in some studies) being the major CaMKII product expressed, along with minor levels of δ3 (δB) detectable by RT-PCR and Western blot analysis. In the present study, we inhibited endogenous CaMKIIδ2 function by using an adenoviral infection approach to introduce a kinase-negative mutant of CaMKIIδ2 that was demonstrated to act as a dominant negative with respect to CaMKII activity. Whereas KN-93 was shown to inhibit PDGF-stimulated VSM cell migration, overexpression of the kinase-negative CaMKIIδ2 potentiated PDGF-induced migration. Furthermore, expression of a constitutively active δ2-isoform inhibited VSM cell migration. The results of these studies necessitate a careful reexamination of the mechanisms by which CaMKII regulates VSM cell migration, cognizant of potential isoform-specific functions and/or functions uniquely related to its autophosphorylation state.
Antibodies and other materials.
Production and specificity of the antibodies used for detection of the δ2-specific isoform of CaMKII and the Thr287 phosphorylated form of CaMKIIδ2 were described previously (20, 31). Polyclonal antibody specific for the hemagglutinin (HA) epitope was purchased from Upstate Biotechnology (Lake Placid, NY) and monoclonal antibody specific for the Flag epitope was obtained from Sigma (St. Louis, MO). Antibody specifically recognizing the phosphorylated form of phospholamban (PLB; PT17), was purchased from Cyclacel (Dundee, UK). All cell culture media and supplies were obtained from Fisher Scientific (Pittsburgh, PA) unless otherwise specified. Ionomycin was purchased from Calbiochem (La Jolla, CA). PDGF-BB and all other chemicals were purchased from Sigma.
Cell culture.
VSM cells were dispersed from thoracic aortas of 200- to 300-g Sprague-Dawley rats as previously described (7). Cells were cultured in combined DMEM/F-12 medium supplemented with 10% fetal bovine serum (FBS) (HyClone, Logan, UT) at 37°C and 5% CO2. Cells from passages 3–10 were used for experiments. Cells were grown to 70% confluence before infection with adenoviral constructs.
Cloning and adenovirus generation.
Mutations of CaMKIIδ2 were engineered using the Transformer Site Directed Mutagenesis kit (Clontech, Palo Alto, CA). Kinase-negative CaMKIIδ2 was generated by replacing Lys43 with an alanine. Amino acid substitution of Thr287 to aspartic acid generated a constitutively active CaMKIIδ2 mutant. M. T. Crow provided adenoviral stocks encoding kinase-negative (Ad-KN:δ2) and constitutively active (Ad-CA:δ2) CaMKII. Adenovirus encoding β-galactosidase (Ad-βGal) was a gift from R. S. Keller. RT-PCR was used (Titan one-tube RT-PCR kit; Roche, Indianapolis, IN) to clone PLB from isolated rat brain RNA. Primers were designed according to the rat PLB cDNA sequence (accession no. L03382.1): upstream, 5′-GAGAGGATCC(814)ATGGAAAAAGTCCAATACCT(833)-3′, and downstream, 5′-GAGAACTCGAG(972)TCACAGAAGCATCACAATG(953)-3′. The amplified product containing the entire coding region was then cloned into pCMV-tag2B (Invitrogen, La Jolla, CA) and subcloned by digesting Flag:PLB cDNA with SnaB1 and Xho1 (New England Biolabs, Beverly, MA) followed by ligation into a pShuttle vector (provided by Dr. Peter A. Vincent, Center for Cardiovascular Sciences, Albany Medical College, Albany, NY) for virus generation. pShuttle:PLB was linearized and introduced by electroporation into BJ5183 cells containing the pAdEasy backbone for recombination. The resulting recombined vector was linearized by Pac1 digestion and transfected into HEK-293 cells for viral packaging.
All adenovirus stocks were propagated by adding small amounts of virus to HEK-293 cells. When cells were ∼50% lysed, cells and media were collected, subjected to freeze-thaw cycles three times, and then aliquoted and stored at −80°C. Titer assays were performed according to the method of O'Carroll et al. (14). All assays were performed using β-galactosidase as an adenoviral control at matching multiplicity of infection (MOI).
Cell lysates, immunoprecipitation, and immunoblotting.
Thirty minutes before experimentation, growth medium was removed from VSM cells and replaced with Hanks' balanced salt solution (HBSS) supplemented with Ca2+/Mg2+ and 10 mM HEPES, pH 7.4. Reactions were stopped by removing HBSS, transferring the dishes to ice, and adding RIPA lysis buffer (4°C, 10 mM Tris, pH 7.4, 100 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1 mM NaF, 20 mM Na4P2O7, 2 mM Na3VO4, 1% Triton X-100, 10% glycerol, 0.1% SDS, 0.5% deoxycholate, 1 mM dithiothreitol, 0.1 mM phenylmethylsulfonyl fluoride, 0.2 U/ml aprotinin) at 0.3 ml/60-mm dish. The lysates and cells were collected into 1.5-ml tubes and then centrifuged at 14,000 rpm at 4°C for 10 min, and the supernatant was collected for Western blot analysis or storage at −20°C.
For immunoprecipitation of PLB, 60-mm dishes infected and treated with an appropriate stimulus were lysed in 500 μl of immunoprecipitate (IP) buffer (500 mM Tris, pH 7.4, 50 mM NaF, 0.1 mM NaVO4, and 0.5% NP-40). Dishes were scraped, and lysates were cleared by centrifugation at 14,000 rpm at 4°C for 10 min. Monoclonal anti-Flag antibody (2 μg) and 40 μl of protein A beads were added to each IP (Pierce, Richmond, CA). After immunoprecipitation overnight at 4°C, they were washed three times the next day in lysis buffer followed by the addition of SDS sample buffer.
Lysates and IPs were resolved by 9 or 15% SDS-PAGE gel and transferred to nitrocellulose. The membranes were blocked in Tris-buffered saline containing 0.2% Tween 20 (TBST) and 5% nonfat dry milk. After blocking, the membranes were incubated in primary antibody for 1 h at room temperature, washed three times with TBST, and incubated with horseradish peroxidase-conjugated secondary antibody (1:1,500 dilution; Amersham) for an additional hour at room temperature, then washed three times with TBST. Membranes were developed using chemiluminescence substrate (Amersham) and exposed to Hyperfilm ECL (Amersham Biosciences, Piscataway, NJ). PLB phosphorylation was quantified by densitometric analysis of the immunoblot enhanced chemiluminescence signals with the use of ImageQuant software (Molecular Dynamics, Sunnyvale, CA).
Additional antibody dilutions used included CaMKIIδ2 antibody at 1:1,000, polyclonal antibody for phosphorylated CaMKII at 1:500, polyclonal HA and monoclonal Flag antibodies at 1:1,000, and phosphorylated PT17 at 1:5,000.
CaMKII activity assay.
Thirty-six to forty-eight hours after infection with adenoviral constructs, 300 μl of lysis buffer (50 mM MOPS, pH 8.6, 100 mM Na4P2O7, 100 mM NaF, 250 mM NaCl, 2 mM Na3VO4, 3 mM EGTA, and 1% NP-40) was added to each 60-mm dish. Lysates were scraped and centrifuged at 14,000 rpm for 10 min at 4°C. Total CaMKII activity in the presence of saturating Ca2+ and calmodulin, as well as autonomous activity, assayed without added activators, was determined as described previously using autocamtide II, a specific peptide substrate (1).
Cell migration assays.
Cultured VSM cells were grown in medium containing 10% FBS (as described in Cell culture) for 36–48 h after infection with adenoviral constructs. All 60-mm dishes were washed once in HBSS, trypsinized, and seeded at 150,000 cells/modified Boyden chamber (PET track-etched, 8-μM pores, 12-well format; Becton Dickinson, San Diego, CA) coated with 3 μg/ml fibronectin (Sigma). Chambers were prepared by adding to the lower chambers medium containing either DMEM with 0.4% FBS (control) or 10 ng/ml PDGF-BB in DMEM with 0.4% FBS. Cells were permitted to migrate for 4 h. The tops of the membranes were swabbed to remove cells, and then cells on the bottom surface of the membrane were fixed for 30 min in PBS containing 4% paraformaldehyde (pH 7.4). Cells were stained for 10 min in Coomassie blue. Filters were removed from the chamber and mounted onto microscope slides on which four identically located fields per membrane were averaged for quantification of cell number.
Adhesion assay.
Thirty-six to forty-eight hours after infection with adenoviral constructs, the ability of the cells to adhere to a fibronectin-coated surface (10 μg/ml; Sigma) was assayed as previously described (3).
Statistical evaluations and comparisons.
All data are expressed as means ± SE. Mean values of groups were compared by ANOVA with post hoc comparisons using the Newman-Keuls test. For all comparisons, P < 0.05 was considered statistically significant.
Expression and characterization of CaMKIIδ2 mutants.
CaMKIIδ2 is the major subunit expressed in cultured rat aortic VSM cells (20). To probe the function of this subunit, two HA-tagged mutants were produced and expressed in VSM cells by using replication-deficient adenoviruses (Fig. 1A). One virus (Ad-KN:δ2) contained a kinase-negative construct as a result of amino acid substitution (K43A) that prevents the binding of ATP (17). The second adenovirus (Ad-CA:δ2) contained a constitutively active construct produced by a charged amino acid substitution at T287D, which mimics autophosphorylation at that site and results in autonomous activity in the absence of bound Ca2+/calmodulin (24).
Preliminary experiments using adenoviruses to introduce green fluorescent protein (GFP) into VSM cells established that infection efficiencies in these cells were essentially 100% (data not shown). Increasing the MOI of both Ad-KN:δ2 and Ad-CA:δ2 resulted in expression of mutant δ2-subunits that resolved slightly larger than the endogenous isoform because of addition of the NH2-terminal HA tag. Infection with adenovirus itself did not significantly alter endogenous levels of CaMKIIδ2, as demonstrated by addition of increasing MOI of Ad-βGal (Fig. 1B).
To determine the effects of expressing these constructs on CaMKII activity, in vitro kinase assays (1) were performed using lysates of control and infected cells (Fig. 2). CaMKII activity was assayed both with and without added Ca2+ and calmodulin to obtain total and autonomous kinase activity, respectively. Infection of cells with 10 MOI of Ad-KN:δ2, which produced approximately fivefold more mutant subunits relative to endogenous subunits (Fig. 1B), decreased total CaMKII specific activity in VSM cell lysates by ∼50%. Basal autonomous activity was not detected in cells infected with 10 MOI of Ad-KN:δ2 (Fig. 2). Conversely, comparable increases in the expression levels of the constitutively active δ2 mutant after infection with 10 MOI of Ad-CA:δ2 significantly increased both autonomous and total CaMKII activity in VSM cell lysates (Fig. 2). The level of autonomous activity observed with this MOI of active δ2 is comparable to the total Ca2+/calmodulin-stimulated activity in control cells. Infection with Ad-βGal did not significantly alter CaMKII specific activity.
To test the actions of mutant constructs on CaMKII activity within intact VSM cells, phosphorylation of a known substrate for CaMKII was evaluated after stimulation of cells with a Ca2+-dependent stimulus (ionomycin). Because endogenous substrates for CaMKII in VSM cells have not yet been well defined, we overexpressed PLB, which is known to be phosphorylated by CaMKII specifically on Thr17 (12, 22) and has been used as a readout of CaMKII activity in the heart (11, 36). PLB is not expressed significantly in these cultured VSM cells (data not shown); therefore, a Flag-tagged PLB cDNA was engineered into an adenovirus (Ad-PLB) for high-efficiency infection and expression. Infection with Ad-PLB resulted in PLB protein expression in the cells, as detected by immunoblotting with an anti-Flag antibody (Fig. 3, A and B). In VSM cells coinfected with control Ad-βGal (MOI = 10) and Ad-PLB (MOI = 160), ionomycin stimulated a significant increase in the phosphorylation of PLB on Thr17 (Fig. 3B), which was inhibited by KN-93 pretreatment (Fig. 3A), validating this as a readout of endogenous CaMKII activity. Introduction of the kinase-negative δ2 mutant resulted in inhibition of ionomycin-stimulated Thr17 phosphorylation by 65 ± 10% (n = 3), indicating a dominant-negative function of the construct with respect to endogenous CaMKIIδ2-dependent substrate phosphorylation. Introduction of Ad-CA:δ2 stimulated PLB Thr17 phosphorylation even in the absence of the ionomycin stimulus, consistent with the constitutive activity of the expressed kinase (Fig. 3B). Addition of a Ca2+ stimulus resulted in further phosphorylation of PLB, consistent with in vitro assays (Fig. 2) indicating that the T287D mutation confers only partial activation of the kinase in the absence of added Ca2+/calmodulin.
Effects of CaMKIIδ2 mutants on Thr287 autophosphorylation.
Autophosphorylation of CaMKII on Thr287 has been shown to be important not only for calmodulin trapping and autonomous activity of the enzyme (9, 23) but also for targeting the holoenzyme (2). To determine the effects of overexpressing the CaMKIIδ2 mutants on Thr287 autophosphorylation, VSM cells were stimulated with ionomycin and the lysates were immunoblotted with an antibody that specifically recognizes phosphorylated Thr287, as previously described (31). In uninfected cells (Fig. 4, A and B) or in cells infected with Ad-βGal (MOI = 10) (Fig. 4B), stimulation with ionomycin resulted in transient Thr287 autophosphorylation, which peaked 45 s–1 min after the addition of ionomycin and fell to near basal levels after 5 min. Pretreatment of VSM cells for 30 min with the pharmacological inhibitor of CaMKII, KN-93, attenuated this autophosphorylation event (Fig. 4A). Conversely, infection with Ad-KN:δ2 (MOI = 10) did not inhibit ionomycin-stimulated Thr287 autophosphorylation. In fact, expressed kinase-negative subunits became phosphorylated on Thr287 in parallel with the endogenous subunits (Fig. 4B). The overexpression of constitutively active CaMKIIδ2 after infection with Ad-CA:δ2 (MOI = 10) blocked ionomycin-stimulated autophosphorylation of Thr287 in the endogenous subunits and, because of the T287D mutation, was not itself a substrate for intersubunit autophosphorylation reactions (Fig. 4B).
Kinase-negative or constitutively active CaMKIIδ2 has no effect on VSM cell adhesion.
To determine whether VSM cell adhesion was affected by the mutant δ2 constructs, cells were infected with either Ad-KN:δ2 or Ad-CA:δ2, and their adherence to fibronectin-coated dishes was assayed (3). As shown in Figure 5, VSM cell adherence increased for 2 h, and overexpression of either the kinase-negative or constitutively active mutant had no significant effects on adherence, suggesting that the effects of each mutant on cell migration in the modified Boyden chamber assay (described below) were not due to changes in adhesion efficiency.
Effects of kinase-negative and constitutively active CaMKIIδ on VSM cell migration.
The effects of overexpressing mutant CaMKIIδ2 constructs on VSM cell migration were evaluated in a modified Boyden chamber assay by using PDGF-BB (Fig. 6A) as a chemotactic stimulus. In control VSM cells infected with 10 MOI of Ad-βGal, addition of PDGF to the lower chamber stimulated a threefold increase in the number of cells migrating to the lower side of the membrane. Expression of the kinase-negative CaMKIIδ2 subunit, which inhibited endogenous CaMKII activity by 65% (Fig. 3), significantly increased the number of cells migrating to the lower chamber under unstimulated conditions and potentiated the response to PDGF (P < 0.05; Fig. 6, A and B). In contrast, pretreatment with KN-93, a purportedly selective CaMKII inhibitor, at concentrations previously shown to maximally inhibit CaMKIIδ2 activity in these cells (1, 31), significantly inhibited PDGF-induced VSM migration in control cells (Fig. 7). Similar results were found with the use of a mixed stimulus (conditioned medium) for chemotaxis (data not shown). Furthermore, acute overexpression of the constitutively active CaMKIIδ2 mutant significantly decreased the number of cells migrating in response to PDGF (P < 0.05; Fig. 6, A and B). The inhibitory effects of constitutively active CaMKIIδ2 and KN-93 pretreatment were nonadditive (Fig. 7).
Although all CaMKII isoforms have conserved catalytic and regulatory domains and therefore have similar kinetic properties, recent studies have indicated significant isoform-dependent differences in subcellular localization and protein targeting (2, 21, 30). Therefore, in attempting to understand CaMKII function in a given cell type, it is important to consider the isoform expression pattern. We (20) previously identified the δ2-isoform of CaMKII as the major subtype within cultured rat aortic VSM cells. To determine specific functions of this subunit, in this study we validated and applied molecular approaches by using CaMKIIδ2 mutants with altered regulatory properties to manipulate cellular CaMKII activity. Because this study, to our knowledge, is the first functional assessment of a kinase-negative CaMKII construct in any cell, the effects of expressing this mutant on endogenous CaMKII activity, including autophosphorylation reactions, were carefully characterized. To provide an unambiguous readout of endogenous or overexpressed kinase activity, PLB Thr17 phosphorylation was used as a specific substrate (11, 36). Our results indicate phosphorylation of PLB by endogenous CaMKII and inhibition of this activity with KN-93 or approximately fivefold overexpression of the kinase-negative CaMKIIδ2 mutant relative to endogenous kinase. Furthermore, this level of kinase-negative mutant expression inhibited CaMKII activity in VSM lysates assayed in vitro. Thus the results of both approaches indicate that overexpression of this construct is dominant negative with respect to CaMKII substrate phosphorylation.
Analysis of CaMKIIδ2 phosphorylation on Thr287, however, indicated that overexpression of the kinase-negative mutant at this level did not block the highly cooperative (17, 23) autophosphorylation reactions and, in fact, that it served as a substrate for these reactions (Fig. 4). Because the kinase-negative subunit is unable to catalyze autophosphorylation, and because these reactions have been demonstrated to be intersubunit within the multimeric holoenzyme (4), these data indicate that the mutant subunit integrates into holoenzymes with endogenous δ2-subunits and suggest proper targeting of the mutant subunits. Conversely, in cells overexpressing the constitutively active mutant, endogenous CaMKIIδ2 Thr287 autophosphorylation was inhibited. This result has a number of potential explanations, including inactivating autophosphorylations catalyzed by the mutant on Thr306/307 in the calmodulin binding domain (19) and/or upregulated phosphatase activities enhancing reversal of the autophosphorylation events. Regardless of the mechanisms involved, overexpressing these mutants in Thr287 autophosphorylation could be an important consideration in interpreting the functional effects of these mutants, as in the present study and in previously published experiments in which acute (13) or stable (15, 35) overexpression approaches were used.
Several previous reports have indicated a role for CaMKII in VSM cell migration (13, 15, 35). A consistent finding confirmed in the present study is that selective pharmacological inhibitors of CaMKII (KN-62 or KN-93) inhibit migration of VSM cells toward chemotactic stimuli (13, 15). These agents act by interfering with Ca2+/calmodulin binding and subsequent activation, including autophosphorylation reactions (Fig. 4A). In the present study, however, introduction of the kinase-negative CaMKIIδ2 mutant into VSM cells enhanced migration toward PDGF (Fig. 6) or conditioned medium. We currently have no definitive explanation for this discrepancy in results with the use of the different approaches. Potential explanations are that KN-93 exerts nonspecific effects on VSM cell migration or that the kinase-negative construct enhances migration through an effect unrelated to CaMKII per se. Another, more interesting explanation that is consistent with both the pharmacological and molecular approaches is that the requirement for CaMKII activation in cell migration necessitates autophosphorylation.
A requirement for CaMKII autophosphorylation in cell migration is also consistent with the results obtained with overexpression of the constitutively active CaMKIIδ2 mutant. In these experiments, infection with constitutively active T287D mutant was titrated to achieve a level of expression that resulted in autonomous activity that was quantitatively comparable to the maximum levels of endogenous Ca2+/calmodulin-stimulated activity expressed in normal cells (Fig. 2). As discussed above, however, the effect of overexpressing this mutant on autophosphorylation of endogenous subunits was inhibitory, and the net effect on VSM cell migration also was inhibitory.
Similar studies using overexpressed constitutively active CaMKII mutants have been published, but with different conclusions. In general, the prior studies found enhanced VSM cell migration (35) or rescue of KN-62 or KN-93 inhibition of PDGF-stimulated cell migration (13, 15). Differences between the current and prior studies may factor into why essentially opposite results were found in our study. In many of the previous studies, the constitutively active construct was stably expressed (15, 35). It could be expected that this approach would result in significant adaptive changes, including the induction of phosphatase activity, as indicated by experiments evaluating CaMKII in VSM cell migration in response to wounding (35). With the use of the more acute adenoviral infection approach, as in the present study, there is less time for such adaptive changes to occur. In addition, in those prior experiments in which constitutively active CaMKII constructs were overexpressed using an adenoviral delivery system, the constructs were mutants of the CaMKIIα isoform (13) and contained a second charged residue substitution (V287D) in addition to the charge substitution at the autophosphorylation site in the α-subunit (T286D). To the extent that unique sequences in the δ2-isoform and/or autophosphorylation levels may be important in holoenzyme targeting, the more densely charged, constitutively active α-subunit may act differently from the constitutively active δ2-construct used in the present study. A potential requirement for CaMKII autophosphorylation in VSM cell migration (discussed above) might be satisfied by the more densely charged mutant and not by the δ2-mutant, which also inhibited endogenous subunit autophosphorylation. Finally, an important limitation of any experiment expressing a constitutively active multifunctional serine/threonine kinase is persistent, unregulated phosphorylation of normal substrates as well as recruitment of nonphysiological substrates.
With the use of new molecular approaches, the present study indicates an important role for CaMKIIδ2 in regulating cultured VSM cell migration. If interpreted strictly in the context of CaMKIIδ2 activity toward substrates other than itself, the results are not consistent with the effects of chemical inhibitors of the kinase. Alternatively, an inclusive interpretation of the results could point to a requirement for CaMKII autophosphorylation in VSM cell chemotactic migration. Although it is speculation at this point, this possibility is consistent with an increasing number of results in other systems indicating the importance of autophosphorylation events in directing CaMKII holoenzyme targeting (2) and enzymatic activity (34). The ultimate cellular mechanisms underlying CaMKII actions on cell migration are also not yet known but could be related to effects of the kinase on the stability and turnover of focal adhesions (32) or on the activity of the contractile apparatus, perhaps by inhibiting myosin light chain kinase activity (28). The present results indicate the need for additional studies to clarify the mechanisms by which CaMKIIδ2 regulates VSM cell migration and whether these results can be extrapolated to other cell systems.
This work was supported by the American Heart Association-Northeast Affiliate Predoctoral Training Grant 0215300T (to P. J. Pfleiderer), National Institutes of Health Predoctoral Training Grant T32-HL-07194, and National Heart, Lung, and Blood Institute Grant R01-HL-49426 (to H. A. Singer).
We acknowledge the technical assistance of Ginny Foster in the preparation and maintenance of cell cultures and thank Wendy Hobb for expert assistance in the preparation of the manuscript.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2004 the American Physiological Society


text:
|29 Sep 2010
Windows 7 x64
New Laptop crashing when going into sleep mode
Hi! First of all, thanks a lot for any help you can provide, I'm having some weird crashes when the computer goes into sleep mode. During normal use, all seems to go fine, I stressed the machine with some 3d renders for a few hours and it stood perfectly, the problem is just when it goes to sleep.
Computer is a Sager 7280 with Windows Ultimate 64 retail, recently installed and just a few programs installed, a RAID 0 of hybrid drives, 12 GB of RAM and an Nvidia 840M with a i7 980
Thanks a lot!
|My System Specs
|01 Oct 2010
Win7 x64 + x86
Also, please remove or update these older drivers that were loaded at the time of the crash. Don't use Windows Update or the Update drivers function of Device Manager.
Please use the following instructions to locate the most currently available drivers to replace the one's that you uninstall OR remove:
How To Find Drivers:
- I have listed links to most of the drivers in the code box below. Please use the links there to see what info I've found about those drivers.
- search Google for the name of the driver
- compare the Google results with what's installed on your system to figure out which device/program it belongs to
- visit the web site of the manufacturer of the hardware/program to get the latest drivers (DON'T use Windows Update or the Update driver function of Device Manager).
- if there are difficulties in locating them, post back with questions and someone will try and help you locate the appropriate program.
- - Driver manufacturer links are on this page: http://www.carrona.org/drvrdown.html
Here's the older drivers (You can look them up here: Driver Reference ).
Please pay particular attention to any dated 2008 or earlier:
wacommousefilter.sys Fri Feb 16 13:12:17 2007 (45D5F401) - Wacom(tablet) Mouse Filter - http://www.carrona.org/dvrref.html#wacommousefilter.sys PdiPorts.sys Thu Nov 16 19:31:16 2006 (455D02D4) - PdiPorts Device Driver - http://www.carrona.org/dvrref.html#PdiPorts.sys
Built by: 7600.16617.amd64fre.win7_gdr.100618-1621 Debug session time: Wed Sep 29 19:35:09.154 2010 (UTC - 4:00) System Uptime: 0 days 5:22:08.137 BugCheck 9F, {3, fffffa800de8e060, fffff80000b9c518, fffffa800c27d380} Probably caused by : usbhub.sys BUGCHECK_STR: 0x9F PROCESS_NAME: System ¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨`` Built by: 7600.16617.amd64fre.win7_gdr.100618-1621 Debug session time: Tue Sep 28 20:10:05.411 2010 (UTC - 4:00) System Uptime: 0 days 0:42:24.394 BugCheck 9F, {3, fffffa800d869440, fffff80000b9c518, fffffa800bea8bd0} Probably caused by : usbhub.sys BUGCHECK_STR: 0x9F PROCESS_NAME: System ¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨`` Built by: 7600.16617.amd64fre.win7_gdr.100618-1621 Debug session time: Mon Sep 27 01:59:40.914 2010 (UTC - 4:00) System Uptime: 0 days 0:20:35.286 BugCheck 7A, {fffff6fc40009f00, ffffffffc0000185, bbcef860, fffff880013e0b30} *** WARNING: Unable to verify timestamp for win32k.sys *** ERROR: Module load completed but symbols could not be loaded for win32k.sys Probably caused by : memory_corruption BUGCHECK_STR: 0x7a_c0000185 PROCESS_NAME: svchost.exe ¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨¨``
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Which of the following example
Which of the following examples were given in support of a bill of rights in Anti-Federalist No. i 84? Check all that apply.
Tutor Answer
Brown University
1271 Tutors
California Institute of Technology
2131 Tutors
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982 Tutors
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1256 Tutors
Dartmouth University
2113 Tutors
Emory University
2279 Tutors
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599 Tutors
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2319 Tutors
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1645 Tutors
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1911 Tutors
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2122 Tutors
Pennsylvania State University
932 Tutors
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My parents live in Jacksonville, and my siblings and I are going to get them some concert tickets for Christmas and would like to get them a gift certificate for a fairly nice dinner as well. Since the concert will be downtown, we figure it would be most convenient to find a restaurant for them around the downtown area as well (or Riverside, Avondale, San Marco, etc.). As for taste, they are not too brave in trying new things and an Italian restaurant, Steak house, etc. type of place would probably be most fitting (vs. say Indian food, Thai, etc.). We are also thinking around $100 for a two-person meal.
Any recommendations would be greatly appreciated! I know Ruth's Chris is downtown, but would like to try something besides that location if possible!


text:
Legal Question in Family Law in Georgia
I currently live in Georgia with my husband, son, and daughter. We have been Georgia residents for a year now. My daughter's biological father lives in Indiana, which is where I originally filed the custody case for her. I am originally from Indiana and my daughter was born there. However, my husband is in the military and we were moved to Georgia in 2011. My daughter's biological father was ordered to pay child support in 2009. Since that time he has made only one payment of $65 in December of 2009. He is now as least $7,500 behind. I want to pursue the child support. However, I want to try to have the case moved to the Georgia court system before I do this so that we don't have to keep driving back to Indiana for court. I was previously told that this shouldn't be a problem seeing as how my daughter and I are now Georgia residents. I currently don't have the money available to hire a new lawyer in Georgia. How do I go about filing a petition to have the case moved to Georgia?
1 Answer from Attorneys
Did you know that his failure to pay child support is a court martiable offense and he can be thrown in military prison and dishonorably discharged. That gives you great leverage. Use it. Speak to his commander. Some commanders fail to act, and in such a case go to their superior and report both of them. As a military dependent, you can receive assistance from military lawyers as well. Use that. You need to be more aggressive and proactive and use the leverage you have. Good luck!


text:
Acrylic on Masonite, 30 x 40 cm
Wednesday, March 28, 2007
"Big Time Wrestling"
Posted by Thorsten Hasenkamm at Wednesday, March 28, 2007
3 comments:
- Matt J said...
Hey Thorsten, really like the 60's style girl & the colour palette you used here. Bravo!
- 5/4/07 16:52
- plutonianode said...
I like all the shapes in this one, and I agree with matt j, the color palette is great.
But Lucha Libre is still my favorite!
You should post your work up at MyToons.com. It's an online community for artists/animators and there's so much you can do with it--it'd be cool if your work was on there, too.
Looking forward to seeing more stuff!
- 16/4/07 23:01
- Thorsten Hasenkamm said...
I'm glad you like it!
- 24/1/08 01:25
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Young Alumni Multiply Gifts with the Westminster Young Alumni Challenge
Westminster Young Alumni Reunion Gathering
Westminster Alumni who graduated between 2000 and 2010 can make their money go twice or even three times as far through the Westminster Alumni Council's Young Alumni Challenge.
To encourage recent Westminster graduates to invest in their alma mater, the Alumni Council will match all gifts made by members of the Classes of 2000 through 2010 to The Westminster Fund and The Westminster Scholarship Fund up to a total of $10,000. Gifts from donors who gave last year will be matched 1:1. Gifts from young alumni who haven't given will be matched 2:1. The result is that a $25 gift can become a $50 or even a $75 gift. All gifts must be received by June 30, 2011 to qualify for the match. As of May 26, 2011, $3,600 has been raised in gifts and pledges toward the match.
Gifts to the Westminster Fund and the Westminster Scholarship Fund support many vital projects, such as:
- Providing financial aid to more than 90 percent of students through unrestricted scholarship
- Underwriting travel expenses for tours and run-outs
- Restoring of the Steinway grand piano in The Playhouse and the Praetorius organ
- Repairing to Scheide Hall's Casavant organ
- Installing of Roland Keyboard Lab System in Cottage 7
- Installing audio recording systems for Bristol Chapel, The Playhouse, Talbott Library and Williamson Hall
To make your gift to the Westminster Young Alumni Challenge, go to alumni.rider.edu/wccgive


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This product is no longer available.
You can try these similar gifts instead.
Fly Casting Lesson in Seattle
Learn the basics of fly-casting from the best anglers around with this two hour lesson. Already have the basics covered? Then expand your skills with a lesson in hauling, roll cast, steeple cast, and mending.


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educational and training services for the mortgage industry, namely, conducting seminars and workshops to prepare individuals to enter the industry
Business in LAS VEGAS, NV
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Finding Trinity
Trinity Anglican Church worships at 929 11th Street, Marysville, Ca. This is just across the 10th Street Bridge (Colusa) from Yuba City. We are one block north of 10th Street. From 10th Street, (Hwy 20) take G street north one block to 11th and 11th west (left turn) to 929 11th at the corner of 11th and J.
Contact Us
Church Office: 530-763-2509
929 11th Street, Marysville, CA


text:
8 sounds
Recorded via Line Out 44.1 kHz 16Bit
Slamming big wooden door in very large room. Rec by zoom H2
Woman screams in a state of pain delusion or psychosis Sony ECM-99 stereo microphone to SonyMD (MZ-N707)
This is made from parts of SqueakyGate.wav by acclivity and crash.wav by sagetyrtle, using Goldwave. Thanks ;) Basic editing only: ...
13.06.2011: about 18.00. courtyard ambience (an old tenement on Gorna Wilda street in Poznan/Poland). sounds of an argument between man ...
Our neighbors fought a lot. Usually at around 2am and it would go on for about an hour or two. ...
Inside Terminal 1 at Heathrow airport 1. At least some announcements give the airport feel. Inside microphones of Sony PCM-M10.
8 sounds


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Saturday, February 22, 2014
Pope Francis: 'I will have to pray for Brazil'
Pope Francis holds a soccer jersey, signed by Brazilian soccer star Pelé, which was received as a gift from Brazil's President Dilma Rousseff during a meeting at the Vatican yesterday. The Brazil jersey reads: "For Pope Francis, with respect and admiration. Edson Pelé." Pope Francis joked that he will have to pray for Brazil to become champion after so many gifts but Rousseff just asked him to remain "neutral."


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- Score:
- rated 4.09 / 5 stars
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- 130,652 Views
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- Genre:
- Action
- sprites
- residentevil
- zombies
- metalslug
Credits & Info
- Uploaded
- Sep 10, 2005 | 3:33 PM EDT
If you liked this, check these out!
Plenty more like this here!
Author Comments
Rated 5 / 5 stars
where i dont see it?
i dont see fio getting fuck.or so u mean shes fuck as in, in trouble.which one is it?
Haha you watched the flash just to see someone get fucked? You are a perverted little asian guy. Hahaha.
Rated 4 / 5 stars
dude wow i didnt see that coming haha fio got fucked
Up the ass
Rated 5 / 5 stars
MAN!this was..wo! pls make a sequel if possible!Anyways did the story finish 100%??? i am not really sure...
Rated 5 / 5 stars
Wow i was very impressed, i must say i have seen a few of these now and they are great, this one seems to be the better but it has a certain style, and very smooth animation which i was very impressed with so nice work indeed...
Maybe bringing the filesize down somewhat if you can, and even a mixture of sprite and flash effects for a different twist on it all...
A great enrty to the series if not the best, nice job...
Not the best. Special Edition is the best. That is until ep.5 comes out.
Rated 3 / 5 stars
Good, I liked it, much better.
This was definitely the best in the series, as this one I enjoyed and it was consistently fun to watch throughout, the lack of events as they entered the room with the boss of Umbrella in was rather dissappointing though, as it seemed like you hadn't put much thought into the story. There were a few bad appearance moments, such as when Fio turned sideways on to the screen and when the background wasn't fully on the screen. Also during the scene where two soldiers were talking to eachother within the room, instead of one of their mouth's moving and then the other's, it was just the one's throughout, which didn't look all that great. Aside from those few moments though it was a good movie and I felt you ended it superbly, you left it in a place where you just knew that what happened next would be great, but unfortunately you don't appear to be continuing this.
Lots Of Love, Rebecca Rose
Well uhhhhh you know uhhhh