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Eat Taste Heal
Thomas Yarema, MD, Daniel Rhoda, and Johnny Brannigan
Suggested Reading for Ayurvedic Yoga Specialist Training with Kathryn Templeton
Eat Taste Heal is a groundbreaking work within the field of holistic health and nutrition, written by a medical doctor, a patient, and an acclaimed chef. A complete guidebook and cookbook in one, it demystifies the ancient healing science of Ayurveda, humankind's most ancient system of healthy living.
The aim of the book is simple: to create harmony within through using common foods and healthy lifestyle practices as medicine. The collection contains over 150 cross-cultural recipes and received a Maverick Award for excellence in photography.
Product Details
Publisher: Five Elements Press
Copyright: 2006
352 pages
8.8" x 10.5"
The authors are a unique trio
Physician, patient, and chef, Dr. Thomas Yarema MD, director of the Kauai Center for Holistic Medicine and Research, created for his patients an understandable dietary program rooted in common sense.
Daniel Rhoda, a patient who found healing through Ayurveda, demystifies the ancient science that helped restore his health.
Chef Johnny Brannigan, an internationally trained Ayurvedic chef, has created more than 150 recipes to empower individual healing through the exploration of taste.Purchase for Wholesale
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By : Mani
On : 2012-12-07 13:19:01
A remake of tamil film Subramanyapura in kannada Prem Adda is nothing in special.
Prem Enterts da scene by Banging Thugs. Melkote song at 55 minute.
It is power monger vengeance three unemployed youths turun murderer of Krishnappa. It is narrated in rugged style with village backdrop.
Two songs... Paduvarally Jathrenaga... , Kalli Ivalu... are well sung. The film is not pleasant to the eyes. It is sweet only when Kriti Kharbanda appears on screen. Nothing big so far and nothing new to the kannada audience.
Buy Movie Tickets
Related News

subset: commoncrawl


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Laptop and iPad case-maker Booq is introducing its Cobra line of bags, meant to offer a luxury feel and a fresh design. The lineup includes the Cobra Case L (a full size briefcase; shown here; $345), Cobra Brief L (a mid-sized briefcase; $255), Cobra Slim L (a slim briefcase; $155), and Cobra Courier XS (an iPad messenger bag; $145).
Each of the bags is made of 1680 denier ballistic nylon with water-repellent coating, then trimmed with Nappa leather inside and out. All buckles and loopholes are emphasized with durable gunmetal hardware, giving each Cobra bag a professional look. Additionally, each bag is equipped with an abrasion-resistant and water-resistant rubberized bottom that provides rugged protection.
All Cobra cases are tagged with a unique Terralinq serial number. Register your bag and you'll have a better chance of being reunited if you ever lose it. The Cobra line is available now.


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Posts Tagged: seder Results 9
Make Your Own Haggadah
On Monday night, the first night of Pesach, many of us will sit around a table telling stories. The primary narrative of the evening — the exodus of the Jews from Egypt — is pretty much the same at every gathering, as is the basic framework of the haggadah from which we'll tell the story. Drink wine, dip greens, break matzah, eat bitter herbs, make Hillel sandwich, drink wine, drink wine. Dayenu! And so on.
Passover At YOUR House?
Every Seder has its own story. There was Passover circa 1960, when Uncle Buddy stood in for Elijah. Or the Passover of 1985, when we started the Seder as we got up from sitting shiva for my father. I will never forget the Passover Seders when each of our children recited the Four Questions for the first time. Or the Passover when our first grandson made his appearance, and we all were transformed in our familial relationships and identities.
I Refuse To Be A Slave During Passover
Passover. The word itself makes me shudder. If this holiday is about celebrating the redemption of the Jewish people and the renewal of a nation, why, year after year, do I feel shackled by its very presence?
Seder Plate Orange: Now At Pottery Barn
If ever there was proof positive that a once-marginal feminist Jewish ritual is now mainstream, this is it: The latest Pottery Barn catalog touts a new seder plate — holding an orange.
Marking the Exodus With Kukush and Calamari
What does the path to freedom look like?


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Love Gift Tag w/ Monogram Cutout
by mortinus, published
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The holidays aren't just about snowmen and reindeer! For some of us, it's about letting those we care about know we love them. This is true for me especially as my girlfriends' birthday is on Christmas :)
Complete (English) Alphabet available! If you don't see your initial, please message me I'd be glad to customize this further for you.
Another entry into my Negative Cutout series, show someone special you care with this red heart-shaped gift-tag!
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Facial Lymphatic Drainage
What is the lymphatic system?
The human body has many systems in place to aid in detoxification, protection and overall health. Two of these systems work directly with the skin (in addition to internal organs) to maintain health and balance.
The circulatory system brings oxygen and nutrients to the skin and pulls waste product from the skin back out to be processed.
The lymphatic system works throughout the entire body, and moves fluid called lymph between the skin and the muscles. It consists of organs, ducts, and nodes that work to assist the circulatory system, transporting a watery clear fluid called 'lymph' that distributes immune cells throughout the body and drains excess fluid and waste proteins from the skin (as well as all the body's cells and tissue.) The lymphatic system helps the skin in it's protection of the body, as the second line of defense from invading viruses and bacteria.
We are like human water beds in a way. When our lymphatic system is healthy, fluid travels up and down, back and forth from one area to another all day long. When lymph gets blocked due to poor lymphatic circulation, we are more vulnerable to illness and infection, and waste proteins start to build up.
One of the skin's favorite waste proteins to use is called melanin (i.e. freckles, hyper-pigmentation, sun spots.) Our skin produces this protein to protect us from UV radiation and 'environmental stress.' If our lymphatic system is not processing the excess protein out efficiently, it can get "stuck."
Another example of "stuck" lymph is puffiness. Usually seen under the eyes, puffiness is a build-up of excess fluid under the skin and on top of the muscle.
What is facial lymphatic drainage, and how does it benefit the skin?
Facial Lymphatic Drainage is a very relaxing form of massage that gently unclogs certain lymph nodes and stimulates natural lymph movement. Using the pads of the fingers to lightly manipulate the fluid under the skin down pathways leading to large lymph nodes on the face, neck and chest, this technique helps to drain excess fluid and waste protein.
When performed on a regular basis (as frequently as weekly for extreme conditions), facial lymphatic drainage can greatly reduce puffiness, hyper-pigmentation, and acne break outs as well as decrease down time and bruising following surgery. It is not only non-invasive, but completely holistic – working with the body's own natural processes and bringing balance to the skin.
What can you do from home?
While manual lymphatic drainage is the most effective way to unclog the nodes and get results, there are ways to maintain healthy lymph activity daily.
Stay active! Especially for those of you who work in front of the computer all day. Walking, running, hiking, swimming and yoga all provide the body with constant movement that can help keep the lymph flowing freely.
Dry brush your skin daily with a soft bristled body brush (link to example) using upward circular movements toward your heart. In order to get the best results, your skin must be completely dry and medium pressure needs to be used.
Also, believe it or not, jumping on a mini trampoline for as little as 10 minutes per day can massage the entire lymphatic system (plus it can be really fun, too.) To answer my client's recent question, unfortunately jumping on the bed does not have the same effect.
bien-être skincare studio offers facial lymphatic drainage that can be incorporated into any treatment for optimal results! For more information, visit the skincare studio


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HI guys!
Just wondering if you guys/gals have xindice ver. 1.1b4 runnning? I have installed the war successfully in tomcat and have added the appropriate env variables. My OS is FC3.
I try to add a new collection by issueing the following command:
./xindice.sh ac -c /db -n test
The error message is:
XMLDB Exception 0: Cannot communicate with the server: http://127.0.0.1:8888/xindice/
The url should be http://localhost:8080/xindice/.
Is there a way to configure that?
Thanks in advace for any info..
Xindice Startup URL (1 messages)
- Posted by: Charles Lee
- Posted on: May 04 2005 05:05 EDT
Threaded Messages (1)
- Changing the server port by Robert Simpson on July 05 2005 16:31 EDT
Changing the server port[ Go to top ]
In the command line tool, you can specify that the server is running on port 8080 rather than 8888. See http://wiki.apache.org/xindice/XindiceFAQ/XindicePort . Better yet, you can set up the Tomcat service on the server to listen on both ports 8080 and 8888. Edit $TOMCAT_HOME/conf/server.xml, copy the "Connector" for port 8080 to add another "Connector" under the "Service", and simply change the port number from 8080 to 8888 in the copy.
- Posted by: Robert Simpson
- Posted on: July 05 2005 16:31 EDT
- in response to Charles Lee


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Just wanted to test Twitter's new tweet embedding feature, y'know.
It's National Pastry Day: a time to remember the millions of brave Danish who gave their lives so I might get a little fatter.
— Homer J. Simpson (@HomerJSimpson) December 10, 2011
Hmm. Not as good as I imagined. :/
It works! Madhawa pointed me to the short codes which enables embedding tweets in wordpress.com sites. Woot!
[tweet https://twitter.com/HomerJSimpson/status/145310559158538240]
The way short codes work is explained here. Now even the previous code is working.


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Today I voted for the Republican fiscal year 2012 budget proposal, H.Con.Res. 34, to reduce spending, save critical programs for our seniors and future generations, and make the necessary reforms that will get our economy back on track. While no budget is perfect, this one takes important steps to putting our nation on sound financial footing.
The Republican budget for FY12 rolls back federal spending to pre-stimulus and pre-bailout levels and freezes it for five years. These are much needed changes to our former unsustainable spending levels. With no changes to those over 55, this budget also saves Medicare for tomorrow's seniors with patient-centered reforms. Importantly, this proposal provides flexibility to governors on Medicaid to make local decisions on how best to care for their residents.
This budget achieves these important goals by broadening the tax base while decreasing tax rates for both individuals and businesses and helping end corporate welfare. All told, this budget proposal saves $6.2 trillion dollars over the next decade. It cuts spending, grows the economy, creates jobs, and secures the future for our children and grandchildren.
Source: http://heck.house.gov/press-release/heck-votes-pass-fy12-budget


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Alexion Pharmaceuticals (NASDAQ:ALXN) is on a very steep upward trend that has taken the stock from under $10 per share five years ago to up near the $85 per share range of today. The stock is also hitting new all time highs on a weekly basis. The company's two closest competitors in the industry are Amgen Inc. (NASDAQ:AMGN) and Baxter International Inc. (NYSE:BAX). Amgen Inc. presently has a five year expected PEG ratio of 1.27 along with a five year earnings growth forecast of 9.4%. Baxter International Inc. at the moment has a five year expected PEG ratio of 1.46 with a five year earnings growth forecast of 9.2%. Alexion Pharmaceuticals is currently priced mid range by comparison with a five year expected PEG ratio of 1.36 but has a significantly higher five year earnings growth forecast of 31.6%. Let's take a look at why Alexion's number have been so good:
I believe the majority of growth is coming from Alexion Pharmaceutical's blockbuster drug Soliris. The drug was originally approved by the United States Food and Drug Administration in 2007 for the treatment of paroxysmal nocturnal hemoglobinuria, which causes the breakdown of red blood cells and eventually leads to anemia. In September of 2011 the Food and Drug Administration cleared Soliris for treating patients with atypical hemolytic uremic syndrome, which eventually leads to kidney failure and death. The European Union followed in November of 2011 with the approval for Soliris in the treatment of atypical hemolytic uremic syndrome. This multi treatment attribute from a single base drug represents exponential revenue growth for the company in my opinion and it doesn't stop there.
Alexion Pharmaceuticals is also studying Soliris for the treatment of several other medical conditions such as acute humoral rejection, myasthenia gravis as well as other blood and kidney disorders, neurological illnesses and age-related macular degeneration. As early as this year the company is expecting to report the results of a clinical trial for the drug in the treatment of STEC-HUS, which is another very rare type of infection. Alexion Pharmaceuticals also reported a profit rise of 82% and a revenue increased 46% in the final quarter of 2011-- beating analysts' estimates.
The company is not only beating analysts' estimates it is also surprising itself. Alexion Pharmaceuticals raised its guidance four times in 2011 and beat these estimates each time. In the fourth quarter of 2011 the company reported earnings per share of $.36 which beat estimates by $.07 per share and was $.14 per share above the year ago period. In addition to expanding its revenue growth through other applications of Soliris the company is formulating a strategy of growth through acquisitions and signing deals to expand its pipeline.
In December of 2011 Alexion Pharmaceuticals announced its intentions to purchase Enobia Pharma Corp. for about $1 billion. The company is paying $610 million upfront and an additional $470 million pending regulatory approval and estimated sales figures of Enobia's drug Asfotase Alpha. The drug is presently in the second of three trials needed to pass Food and Drug Administration approval and showing strong results in the trials. Enobia furthermore, focuses on treating rare diseases and should integrate well with the Alexion Pharmaceutical's business model. I think the success of this drug will, to an extent, quell the concerns of many analysts that the company is relying on just Soliris for its revenue growth.
Alexion Pharmaceuticals also recently announced that it intends to acquire Taligen Therapeutics. This purchase also comes with contingency payments and an upfront cash payment of $111 million for 100 percent of Taligen Therapeutics' equity interests. The contingency payments are based on clinical efficacy of six products Taligen Therapeutics has in the pipeline and milestone payments in the drug approval processes in both the United States and the European Union. In my opinion these two types of acquisitions (Enobia, Taligen) limit Alexion Pharmaceuticals risk significantly but still represent a nice payoff upon completion of the approval process.
The company is extending this reduced risk, purchasing strategy and intensifying its global outreach with the purchase of patents and assets from Orphatec Pharmaceuticals, in my opinion. Orphatec Pharmaceuticals is a privately held biotechnology company that is based in Cologne, Germany. Alexion Pharmaceuticals is putting about $3 million upfront with contingency payments paid upon certain development, regulatory, and commercial milestones. The two companies will also be collaborating in research to accelerate development of the investigational therapy.
A little charity can go a long way. This is especially true when you are trying to brand your product and your company's reputation in a positive light. Most company's give lip service to charity in general and try to deceive the public into believing it is something that it is not-- this strategy usually backfires when the facts are discovered, in my opinion. Alexion Pharmaceuticals is putting its money where its mouth is, on the other hand, and is proving the fact that a good deed does not go unpaid. Since 2010 the company has been providing Soliris free of charge to E coli victims in Germany. Enterohaemorrhagic Escherichia coli is a rare form of E coli that produces a Shiga toxin that damages the liver by affecting blood cells and is usually fatal. Through its German subsidiary Alexion Pharmaceuticals has been providing victims of this disease with Soliris for free and in return has foregone the costly clinical trials required by regulators to prove the drug safe and effective. This act of good will was further sanctioned by the United States Food and Drug Administration when in June of 2011 the administration put the approval process for Soliris on the fast track for treating patients with atypical hemolytic uremic syndrome.
Atypical hemolytic uremic syndrome is a rare genetic disease that kills more than 50% of the people who have it. The Food and Drug Administration approval of Soliris "… marks the most important advance that has been made for patients and families with this disease" according to the Director of Pediatric Nephrology at Emory University and Children's Healthcare of Atlanta. A little charity can indeed go a long way once you stand back and look at the big picture, in my opinion. For these reasons, I recommend buying shares of Alexion Pharmaceuticals today.
About the author:
I fundamentally analyze every business from the top down.
In my personal life, I have a strong Jewish faith and enjoy playing Scrabble and entrepreneurship.


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Selected as Party of the Day on December 27th!
2013 New Years Mustache Bash
Party Recap
I styled a party for our upcoming New Years Eve 2013 Mustache Bash! The inspiration came about from a saying...... "Keep Calm and Stache On."
I have planned this year's party to be kid friendly so my 8 year old son can be included in the evening festivities.
I love the colors black and silver so I incorporated a little gray to represent silver in my 2013 Mustache Bash Printables.
Wanting a kid friendly snack and not a whole lot of sugar before and after midnight I came up with the idea of the popcorn cups. They are a much healthier option than cupcakes or a cake. I don't want to exclude all sugar goodies so cookies, candy coated sugar sprinkled pretzels, chocolate bars, kisses, miniatures and nuggets will coordinate very well with the saltiness of the popcorn.
I thought the Martinelli's Sparking Cider would be a nice beverage touch to the table and is also non-alcoholic and kid friendly. The plastic champagne glasses detach at the base so I used a mustache straw hugger in between the base and the cup. I also coated the base with a little glitter for that extra sparkly touch.
I used silver and black glitter foam sheets and craft sticks to create the mustache props. These are so much fun and a must have!
I had planned to use noise makers, as I always do every New Years Eve, but this year I have opted for bubbles. My son loves bubbles and noise makers but I thought we could change tradition a little using bubbles....hence the saying that inspired this party idea "Keep Calm and Stache On."


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when i try to run msconfig It tels me it cant find msconfig.
I do not know how or why it dissapeard .
Can i get it back ? How ?
Instead of trying to run it, have you tried to search for it?
Have you actually looked in C:\Windows\System32 folder to determine
whether or not the file (msconfig.exe) is truly missing?
It might simply be that your Path Statement is screwed up, and
lacking the normal entries for WinXP's system folders. Therefore, WinXP
doesn't know how to find the applications.
It should look something like this:
To edit the Path, right-click My Computer > Properties > Advanced >
Environmental Variables. In the System Variables windows, highlight
Bruce Chambers
Help us help you:
They that can give up essential liberty to obtain a little temporary
safety deserve neither liberty nor safety. ~Benjamin Franklin
Many people would rather die than think; in fact, most do. ~Bertrand Russell
The philosopher has never killed any priests, whereas the priest has
killed a great many philosophers.
~ Denis Diderot
Look for the file in: C:\WINDOWS\PCHealth\HelpCtr\Binaries\msconfig.exe if
the file is missing, it may have been deleted by a spyware or trojan.. so
scan the system with an antitrojan-spyware, and get a copy from another
system or google for a download and paste it in the Binaries folder. If you
can't find a copy of the file, someone here can upload one and provide you
with the link to download it.
Skip the previous filehost link
msconfig.exe can be expanded from the original CD. No need for anyone to
upload anything.
Open the command prompt window, insert the XP CD and enter:
Disclaimer: The information has been posted "as is" with no warranties or
guarantees and doesn''t give any rights.
Or you can use Recuva or any other deleted files restore application to
recover the missing file
Thanks for the tip, it is appreciated.. but suppose the OP does not own a CD?
All systems should have some method of restoring the OS, whether it's a
hidden partition or a disk.
If they don't have a disk, they could contact the manufacturer and get one
from them, they may have a hidden partition that they can access to obtain
the file.
Having no disk makes minor repairs almost impossible.
Disclaimer: The information has been posted "as is" with no warranties or
guarantees and doesn''t give any rights.


text: Normally, the shit that goes down between Spencer and Heidi on The Hills seems so fake and contrived, but on last night's episode, when Spencer crashed Heidi's "girls night out," things actually got real, for once, on this "reality" show. Seeing your ex (even planted by television producers) socializing with other girls in a bar right in front of your face is pretty torturous. Heidi's shrilly freak out of, "They're taking shots!?" is totally the new, "He's a sucky person!" or "You know what you did!" Clip above.


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Activision confirms Radical staff reduction, Luxoflux closure
[UPDATE] Publisher's "realigning [of] resources" sees Prototype staff culled, Transformers studio shuttered; Guitar Hero rumored to be taken from Neversoft.
[UPDATE] Publisher's "realigning [of] resources" sees Prototype staff culled, Transformers studio shuttered; Guitar Hero rumored to be taken from Neversoft.
Valve cofounder to be honored for creating Steam at March developer conference, as Marble Madness creator is honored by Academy of Interactive Arts and Sciences.
New handheld's debut accompanied by first PSP Minis, debuts of PixelJunk Monsters, Thexder Neo, sales on Puzzle Quest, Prinny, and more; full list and price breakdown inside.
Naughty Dog founders Jason Rubin and Andrew Gavin start Monkey Gods, a new casual game development company specializing in 360, PS3, Wii, iPhone, and Facebook games.
First X-Men: Origins movie tie-in coming to PS3, PS2, PSP, PC, 360, Wii, and DS; will debut alongside film inspiration in May 2009.
Crash of the Titans, Dead Head Fred, The Simpsons, Witcher, and World in Conflict nominated for inaugural 2008 Videogame Writer Awards.
Take two for Assassin's Creed.
You'll never guess who crashed the party.
The week's headliners joined by Namco Bandai's Ace Combat 6, Naruto on the Wii and DS, Eye of Judgment, and Zack & Wiki, among many others.
The Orange Box take two.
Half-Life's Orange Box opens up in Australia.
Headliners joined by Spider-Man: Friend or Foe, FlatOut 2, Crash of the Titans, and Chibi-Robo on retail shelves this week.
In this clip, Crash takes his new suit out for a test drive.
Can Crash collect 5,000 Green Mojo before time runs out?
Crash brushes up on his smashing skills in this movie.
Take a look at Crash as he smashes his way through a cave behind a waterfall in this clip.
No forum topics for Crash of the Titans yet. Want to start us off? Create a new topic.
Use your keyboard!
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text:
It's amazing how much hard work goes into designing products. It starts with an idea or inspiration, then drawings, schematics, material selections, tweaks and changes, and then finally production. I have loved seeing each one of my designs become reality with my partner companies: CR Laine, Duralee, Woodbridge, and Soicher Marin.
But do you know what is REALLY a dream come true? When you see those designs on retail floors all around the country or in people's actual homes! That is AH-MAZING! And I love it even more when retailers and designers share those photos with me on Instagram. With all the variations and customizations available from each of my partners, there are some fabulous looks being created with my designs.
For example, look how amazing my Marguerite sofa looks in my favorite color! And then it looks completely different (and just as FAB) in bright orange below!
It's really special when a retailer gets so excited that they can't wait to show you what they've unwrapped, like when the retailer posted the photo below on Instagram.
And then there are the designers who have put my products into their clients' homes – it is SO exciting to see what they choose and how they use them.
Once my Duralee fabrics are officially on the market next month, I can't wait to see how retailers, designers, and consumers will want to use them for their own projects and designs!
So to everyone who has supported me and my partner companies – thank you for making my dream come true! And keep showing me your fabulous photos on Instagram by tagging me with @tobifairley!


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FERRARI'S FELIPE Massa hassles Lewis Hamilton on the arm, sarcastically saying to Hamilton: 'Well done,Very Good Job'.
During his Post-Qualifying & Race Interview, Massa accused the McLaren Driver of 'not using his mind' after Hamilton attempted to Pass him on the Track in Qualifying whilst on their out lap.
The two later had a collision in the race at Turn 4,which resulted in Hamilton losing his Front Wing & Massa getting a puncture: 'Don't touch me!'


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SCA Tork releases green business survey
PHILADELPHIA — SCA has announced the release of survey results showing an uptick in consumer spending on green products and services, according to a press release.
This year''s results were debuted for World Environment Day, which was held on June 5, a United Nations (UN) observance of environmental issues, the release stated.
According to the release, the survey found that 77 percent of American adults purchase green products/services, including 57 percent who do so because they believe it''s better for the environment.
These results, as well as those from the 2009 and 2010 surveys, showcase sustainability as a new way of life — with both of the previous surveys revealing over seven in 10 adults continued purchasing "green" products and services, even during the down economy, the release noted.
"The results stemming from our annual research continue to confirm that the use of responsible products and services is becoming a core value and a driver of behavior," said Mike Kapalko, SCA Tissue North America sustainability marketing manager.
"People know how important their purchasing decisions are and are ensuring they make the best choices for themselves and the planet while doing day-to-day activities like shopping and dining out," Kapalko added.
Click here to read the complete release.


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Confirmation: Apple Will be Releasing Lower Range Models
BY ETrade Supply | May 6th, 2013 | 5 | 0
BY ETrade Supply | May 6th, 2013 | 5 | 0
They need to make the iPhones different from each other, making them thinner and having a big screen dot make any difference… Look at the iPhone 4 and 4S… Not much difference except built in Siri and no random black line near the earphone jack. When the iPhone 5 was advertised.. It showed a laser keyboard and awesome graphics.. And it's never happened?? The iPhone world will get boring if they can't come up with something that other major phone companies can't do.
they never advertised the iphone 5 having a "laser key board" or "awesome graphics". do you mean consents?
Ok thanks to show this thread to me ;..////………
I hope they offers NFC, otherwise it does'nt matter.
©2016 ETrade Supply Blog All Rights Reserved


text:
Israeli DM: Reaching peace deal with Palestinians soon - "fantasy"
A peace agreement between Israel and the Palestinians will not be reached for at least three to five years, an Israeli newspaper quoted Israel's defense minister as saying. The Israeli daily Yedioth Ahronoth said on Friday that in private conversations, Defense Minister Ehud Barak said the idea of reaching a peace deal with the Palestinians anytime soon is a "fantasy" and that Israel will not withdraw from the occupied West Bank before finding a solution to Palestinian rocket attacks, "which will take between three to five years".
Barak also quoted as saying he would not approve the removal of roadblocks from the West Bank, despite assurances given this week by Israeli Prime Minister Ehud Olmert to Palestinian President Mahmoud Abbas that hundreds of checkpoints would be removed. According to Barak, Olmert's meetings with Abbas will not lead to a final peace accord.
"What will determine the situation in the end is if Abu Mazen (Abbas) and (Palestinian Prime Minister) Salam Fayyad are capable of implementing anything in the West Bank," Barak is quoted as saying.
Top Headlines
- Abed Rabbo: Barak-Arafat Summit Soon, but no Swift Peace Deal
- Olmert: Peace with Arabs and Palestinians could be reached in five years
- Arafat orders to stop attacks against Israeli civilians as hopes to soon end Nativity Church siege dissolve
- Sharon: International peace conference to be held soon; Report: U.S. considering "new ideas"
- Report: Israel, Palestinians in New Effort to Forge Peace Deal


text:
Baylor Regents Approve New Ph.D. Program in School of Education, Announce Major Gift for Getterman Softball Practice Facility
Media contact: Lori Fogleman, director of media communications, (254) 710-6275
- Board also approves Baylor tennis renovation project -
The Baylor University Board of Regents today approved a new doctoral program in curriculum and teaching in the School of Education that will address the growing need for more high quality university-based teacher educators to prepare the next generation of teachers for public, private and Christian school service.
The board also announced a significant naming gift from Baylor alumni Ted and Sue Getterman of Waco, Texas, for the Getterman Softball Practice Facility. In addition, Regents approved the renovation of the Baylor Tennis Center building.
Students in the new Ph.D. program in Baylor's School of Education will be able to specialize in one of several fields within curriculum and teaching, such as mathematics education, social science education, science education, and other high need fields. Once they complete the program, the graduates will serve simultaneously as teacher educators and educational researchers whose work will impact local schools. Students will be able to enroll in the new doctoral program beginning in Fall 2009.
"Baylor's School of Education has a long history of commitment to and recognition for preparing highly effective teachers for Texas schools and beyond," said Dr. Jon M. Engelhardt, dean of Baylor's School of Education. "Logical next steps are to add the preparation of first-class faculty, who will provide leadership for teacher education at other universities, as well as join in Baylor's research efforts during their doctoral studies to study critical factors in high-quality teacher preparation and P-12 schooling. The addition of doctoral studies will not only meet Baylor's mission of providing worldwide leadership in selected areas of expertise, but the presence of doctoral students will add value to our undergraduate teacher education program and work with local schools."
Baylor's School of Education will continue to offer the Ed.D. program, which focuses on the advanced preparation of educators who plan to continue their practitioner-based positions in schools, such as curriculum directors, school principals, superintendents and classroom teachers.
Construction on the two athletics projects approved today by the board will begin in May.
The Getterman Softball Practice Facility will be constructed adjacent to Getterman Stadium, which the Gettermans made possible in 2001 with the largest gift to women's athletics in Baylor history. With the addition of the Getterman Softball Practice Facility, the Gettermans generously affirm their commitment to women's athletics at Baylor.
"The Gettermans are special friends of Baylor athletics and among our most generous supporters," said Ian McCaw, Baylor's director of athletics. "We are excited that this wonderful gift of an indoor training building will further enhance Getterman Stadium, the nation's finest softball facility."
"We extend our deepest thanks to Ted and Sue Getterman for their wonderful, real life example of servant-leadership, and for their desire to see women's athletics prosper, especially Baylor softball," said Dr. David E. Garland, Baylor's interim president and dean of George W. Truett Theological Seminary. "Their dedication to Baylor and to our students is an inspiration to all of us."
The Getterman Softball Practice Facility, a multi-purpose training facility, will feature retractable batting cages, pitching areas and other player development resources that will continue to help shape the Baylor softball program into a premier program in women's collegiate athletics.
"Because of the generosity of Ted and Sue Getterman, the top softball facility in the nation is getting better. We are moving the bar even higher with this generous gift," said Glenn Moore, Baylor's head softball coach. "This facility gives our athletes the opportunity to train year-round, both defensively and offensively. No program in the country will have better training facilities than the Baylor student-athletes will have, thanks to Ted and Sue Getterman."
The Baylor Tennis Center building project will include major renovations to the men's and women's locker rooms and lounges, the visitor's locker room and the video room, as well as other renovations to coaches' offices and the building foyer.
The new facilities and degree program represent the latest examples of Baylor's continued forward momentum in strengthening its leadership position as a top Christian university, said Dr. Howard K. Batson, chair of the Baylor Board of Regents.
"Even as we face a very difficult economy, regents heard multiple reports this week that demonstrate that Baylor is continuing to make remarkable progress on multiple fronts. We are optimistic about our future and have every reason to believe that Baylor's upward trajectory will continue," Batson said.
More News ...


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text: Jacobsen Pilot Service Inc., Long Beach Calif. Received a new 54-ft. (16.4-m) welded aluminum pilot boat from Hike Metal Products Ltd. in Wheatley, Ontario, Canada this past July. Hike Metal was approached in the early summer of 2002 after Jacobsen Pilots services owner Tom Jacobsen had conducted a thorough review of the market and read an article about a new Pilot Boat Hike had just built and delivered to Atlantic Canada. While Jacobsen needed a new vessel for the growing demand for Pilot services in Long Beach, he also wanted a modern vessel, specifically designed for function, good visibility, ergonomics , and built to suit their operational requirements out of Long Beach, which is the second largest container vessel port in the U.S. The ships calling the Port of Long Beach today are larger and the pilots have to board further out to sea, also the rush periods now demand a bigger boat with more seating for pilots. After 9/11 Jacobsen Pilots partnered with the U.S. Coast Guard and the Sea Marshals to help deliver them to the incoming ships. The new pilot boat fulfills this mission when required. Hike Metal, now celebrating its' 40th anniversary, provided them with an ideal craft . According to Tom Jacobsen, president of Jacobsen Pilot Service, the new boat was built to meet the growing needs of his company's services to the Port of Long Beach. With their speed and high level of seaworthiness, the new pilot boats are equipped to meet Jacobsen's piloting needs; sometimes dispatching up to eight pilots on the boat during the port's busy mornings. "The business has changed and we're now boarding ships further out at sea," Jacobsen said. The hull form is based on a well proven , standard advanced chine hull design developed years ago by Hike utilized in the construction of its' 30ft to 70ft Patrol boats, currently used on both the Great lakes and inshore coastal operations in North and South America. Final design details and engineering was carried out by EYE Marine Consultants Inc. in Dartmouth, Nova Scotia, Canada. The deep-V hull provides smooth fast and responsive handling while the flattened side chine's afford excellent lateral stability , lift and further help knock down spray. The structural design is based on LRS Special Service craft rules while ABYC rules govern systems and outfit. Careful attention was paid to weight control , to produce a robust and yet sea-kindly craft fit for the demanding role of pilot transfer. The vessel is designed to have a minimum service life of 30 years. Maintenance and service access was of paramount importance to Jacobsen Pilot Service Inc. and these consideration were maintained during all aspects of the design and construction process. The interior fit-out consists of a lower deck accommodation with day berths for four crew, a head , and a small galley area. Access to the engine room is also located there through an oversize bulkhead door allowing easy removal and replacement of large components during future overhauls. The engine room is spacious and well laid out, with ample space over the engines, for easy access and maintenance tasks. It is fitted with a manually operable CO2 system. The propulsion engines can be removed through a soft patch in the pilot cabin floor and cabin deckhead. A single 12 kW Northern Lights generator c/w sound shield provides AC power. All machinery is flexibly mounted for noise and vibration attenuation. In addition the engine room received an insulation system, which resulted in sound levels not exceeding 75 dba anywhere outside the machinery space. The ballast tank system is installed on the vessel centerline forward and aft and can be used for the weight and for trim adjustment in certain conditions. Stern gear consists of Aquamet S.S. propeller shafts , Duramax shaft seals, Vulcan flex couplings and Hall & Stavert five-blade propellers. Steering gear is electro/hydraulic type 4 station provided by Jastram Technologies.A unique portable operating station c/w jog steering and engine controls is fitted inside a watertight station on the stern deck for use in a man overboard emergency. A very functional elevating platform for man overboard retrieval is fitted at the transom. Welded aluminum bollards are fitted both sides along with a fixed towing post on the foredeck and a portable tow post aft. Since the boat was delivered to Jacobsen Pilot Service this past July, according to Tom Jacobsen, it has not left any room for disappointment. "We're very pleased with Hike and were impressed with their commitment to quality boatbuilding," he said.


text: Nathanael Nerode <firstname.lastname@example.com>: > I'm thinking of licensing a program under the GPL, but I dislike the > FSF's overly restrictive concept that 'dynamic linking is modification'. > > I want my program (and any derivative works) to be allowed to use > *accurately documented and published* interfaces to proprietary > (or any other) libraries or programs. I have occasionally searched for the same thing myself, without any great success. The LGPL seems about right in spirit, but it explicitly talks about the work being a "library", which makes it hard to apply it the other way round, though you could design your program to make everything look like a library. Recently someone asked my permission to use in an LGPL library a bit of code that I had contributed to a GPL project. It was easy enough to say yes, but perhaps I should have dual-licensed it in the first place as my file was providing library-like functionality and I might not have been contactable later. Also, other people might have made significant contributions to the file using the same licence. Edmund


text:
You know you have been single too long when...
Is you mam telling you to get back in the saddle? or do you dread the thought of 'getting to know' a new partner? Could be that you've been out of the relationship game too long.
Maeve Higgins
Perhaps when you get used to doing whatever you want, whenever you want, you've been single for too long. The sign of a super-long-term relationship is twitchy little ears and large, darting eyes, constantly seeking approval and permission from their mate.
Take a cinema trip, for example. Do you want to go and see 'Django Unchained'? This afternoon?
First of all, no. It is a disgrace to go to the cinema in the afternoon. You could be doing so many other bits and pieces: cleaning the bathroom, applying for jobs, getting your back waxed.
Second of all, no way. I don't want to see 'Django Unchained' – too violent. Remember when I tried to watch his last one? I nearly got sick. And, the cost of it! The tickets would be €13 each, let alone the popcorn. No. Just an extortionate price altogether.
Aren't we saving for a house? Sometimes I worry that you're just not committed to finding the perfect three-bedroom semi in Drogheda. What we will do is get a DVD this evening instead. Maybe 'Twilight: Breaking Dawn'. It's very well made, apparently; we can both enjoy that.
There's a chance you've been single for too long if you don't see your whole life playing out ahead of you. Maybe you'll still get to live the dream – get married, have children, watch 'The Late Late Show' in a chair until you die.
Or maybe, you'll figure something else out. You'll fall in love a few times. There won't be The One; there'll be The Ones. All Different Ones. Every Single One.
Now, I'm not advocating promiscuity here – more a giddy feeling of potential excitement around every corner. Well, maybe a tiny bit of promiscuity, just a healthy level. I'm not sure what that level is – I will check with the STD clinic and get back to you.
It's handy having a boyfriend or a girlfriend with a great record collection and an even better selection of cool and funny anecdotes. Listen to their favourite songs and make them listen to your favourite songs.
If they wear skinny jeans and check shirts, you can do the same and everyone will think that's just your thing. Insert yourself into their memories and opinions and pass them off as yours. Simply borrow the best parts of their personalities and adopt them as your very own.
You've been single for too long when you begin to develop your own sense of curiosity about the world and strive to experience life on your own terms, so be careful. Latch on to someone, quick, or you won't know who you are.
Una McKevitt
You know you've been single too long when you make a show called 'Singlehood'. The truth is, I've been in and out of relationships for so long that the notion of being single was really scary, so I think the idea was my way of trying to embrace it.
I'm a person of extremes, though, and have gone from being really anxious about being in a relationship to really anxious about giving up being single.
I need to find a happy medium, so maybe a long-distance relationship would work.
You know you've been single too long when your own mother tells you to get back on the saddle.
I've a 'friend' in her mid-30s, let's call her Fiona. She's a single parent and her mother is dying for her to meet someone, settle down and have more kids. She'd quite like that, too – a househusband would suit her nicely, but house-husband material is fairly thin on the ground, unless she goes for someone who can't leave the house for legal reasons.
Anyway, recently she was out there dating and there was one guy she'd made the mistake of telling her mother about. He had texted to ask her out again and she was ignoring the text, which got her mother into a flap – she couldn't understand why Fiona was letting this potential husband slip out of her grasp.
Exasperated, my friend eventually said, "Listen, Mam, there's no point; he's only after one thing and even that he's not much good at."
To which her mother replied, "Fiona, you'll never meet anyone with that attitude."
You know you've been single too long when you'd rather share your bed with an animal. Of the furry variety.
Dogs are great bed companions. I honestly can't think of anything better than spending the weekend babysitting a friend's dog, buying a bag of Haribo for myself and a pig's ear for him/her and taking to the bed at around 9pm for a movie and a few episodes of 'Dexter' or 'Once Upon a Time'.
I get a lot of stick for this. It's sad, I know. It's also very telling that I'd rather 'borrow' a dog than commit to one full- time. I think, well, when I buy a house, I'll get a dog.
But a mortgage is another fairly hefty commitment that requires a certain degree of wealth or naivety, and so borrowing dogs, renting apartments and hoping neither of these shows is ever cancelled is what my future looks like.
You know you've been single too long when you start avoiding people you find attractive.
This happens very occasionally because I'm fairly self-absorbed, so I don't notice people as much as I should, but when I do meet someone I find attractive, I find myself getting nervous – not in a 'Oh, my beating heart' kind of way, but more in a 'I could do without the distraction' kind of way.
Distraction from what, you might ask?
Not a whole lot, to be honest, but recently pottering around on my own, meeting people through work and the odd social occasion, and borrowing dogs for sleepovers, has given me a kind of contentment that honestly none of my previous relationships have.
And I'd like to continue this way for another while.
I'll just have to ignore all the beautiful women throwing themselves at me.
Joanne McNally
You know you've been too single when you begin making enquiries about the third spot in your parent's three-man grave plot they have lying dormant in our local cemetery. I didn't realise until recently that sharing with them was even an option.
I was thrilled to hear that there were an extra few metres up for grabs.
Having been single for six years, any romantic thoughts of meeting my ideal man, convincing him through a cloud of cigarette smoke that I'm wife material and eventually boasting about my own family plot with said husband, had long dissolved away, and now here I was, hurtling towards my 30th year, with no accommodation booked for the afterlife.
My attempt to call shotgun on the third spot was met with boredom from my mother and a look of alarm from my unmarried elderly aunt.
You know you're single too long when you consider fabricating a passing fling to avoid awkward lulls in conversation when friends ask you if you're seeing anyone.
It's a lull you feel obliged to fill with a quick change of topic or some performance art. Stilts are cumbersome, whereas a lie fits easily on the tip of your tongue, regardless of its size.
Friend, tentatively: "Seeing anyone?"
Me: "No."
Friend: "Aaah, sure."
Queue lull.
"Me: "Gotcha!"
Friend: "Sorry?"
Me: "Only messin'. Course I'm seeing someone – yeah, he's deadly, total ride. We're mad about each other, early days but look, no more than yourself and Fergus, when you know, you know."
Maybe now you'll invite me over to dinner, you smug bint.
You know you've been single too long when the thoughts of 'getting to know someone' fills you with nothing but dread. I was sitting opposite a couple on the Luas recently whose stilted conversation and nervous laughter told me they were at that awkward 'getting to know each other' phase.
This is totally grand when you're swinging out of the Globe on George's Street at 2am and your body is 12pc water, 88pc Kahlua. But take away the booze, dim lights and music, and stick us on a quiet Luas carriage in broad daylight, with a 45-minute commute ahead of us and 100 sets of ears beside us? Zero interest.
I watched the couple struggle through the 30-minute journey and – rightly or wrongly – thought to myself, 'Thank God I'm single'.
You know you've been single too long when the director of a show called 'Singlehood' playfully refers to you as her 'muse'. When Una told me she had an idea for a show about single people and asked me would I be interested in getting involved, we were standing outside the dance tent at the Electric Picnic, naturally, we had a few drinks taken, I'm not sure she'd finished her sentence and I was already announcing our collaboration.
Up to this point, Una was the very self-assured, opinionated, mildly terrifying older sister of my best mate and lifelong lady companion Aine. Growing up, I had been largely ignored by Una, except for one heated debate years ago over the Catholic Church. I'm confident we had a drink taken that night also.
Anyway, I knew how good she was, I loved her work and also felt certain that the show would culminate in some love-struck young fella, bewildered by my single status and hypnotised by my glitzy jewellery, storming the stage and sweeping me away.
I had no idea at that stage of the shady secrets I would end up admitting on stage. The term bargepole comes to mind, and not being touched with it.
'Singlehood' plays at Vicar Street, Dublin on March 1 & 2
Irish Independent


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text:
Arts and Crafts in the Park
On July 31, the Detroit Lakes Chamber of Commerce will host its 33rd annual Arts and Crafts in the Park. The event will take place in the City Park, near the Pavilion, and will run from 9 a.m. to 5 p.m.
"It's a day where crafters from all over the area come and display homemade products that they produced themselves," said Marcus Lacher, who runs the show.
Arts and Crafts in the Park will feature a variety of crafts and arts, including quilts, hand-carved wood decorations, and clothing.
"Everything's required to be homemade," said Lacher.
"That's one of the things that's unique about this particular show, he added, noting that "it's not a juried show, but there are not supposed to be commercial items."
In spite of the abundance of artistry at this year's show, there is sure to be something for everyone -- even people who don't have an interest in art.
"Personally, I always look forward to the food," Lacher said, adding that the show always hosts "quite a few vendors."
Lacher has three words of advice for anyone who decides to check out Arts and Crafts in the Park, no matter the reason: "bring your checkbook."
Admission and parking are free.
For more information, contact the Chamber of Commerce at (218) 847-9202. -- Nathan Kitzmann


text:
Britney Lands in Burbank
Britney Lands in Burbank
We were wondering why all was quiet on the Britney front. Looks like she made a quick trip home to see her mother in Louisiana. Britney was seen exiting the plane with her two sons, Jayden and Sean P. Word as they headed directly to a recording studio in Burbank. Could Britney be hearing our cries? Did her mom talk some sense into her? Will she learn to work hard and be a good mom? Stay tuned....We're hoping to report something positive soon.
UPDATE - Unfortunately we are sad to report that Britney went back home to her aunt's funeral. Our thoughts are with her and her family.
For more images of Britney and her boys just
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The Oconee County Sheriff's Office reported the following incidents:
FRAUD: On Sept. 16, a resident of Lois Lane, Watkinsville, reported she found three different fraudulent transactions on her credit card amounting to about $400.
JEWELRY STOLEN: On Sept. 16, a resident of Moreland Heights reported that she found $6,250 worth of jewelry stolen from her home. The woman said she had surgery recently and the theft may have occurred during that time. Some of the jewelry also has sentimental value.
PILLS STOLEN: On Sept. 17, a former resident of Moreland Drive, Watkinsville, reported he moved from this residence in August, but his medicine from the Veterans Administration was shipped there by UPS. The man said someone may have forged his name to receive the medicine that included 84 pills of Methadone.
THEFT ALLEGED: On Sept. 15, security at Walmart detained Joshua G. Shelton, 17, of Branch Road, Bishop, for shoplifting after he took some window chalk from the store.
MAILBOX DAMAGED: On Sept. 17, a resident of Essex Road reported that during the night someone damaged his mailbox possibly with an object like a baseball bat.
SIBLING DISPUTE: On Sept. 20, a deputy was dispatched to Rankin Road, Watkinsville, where two siblings, both juveniles, were in a dispute and one complained she had a hurt jaw.
BURGLARY REPORTED: On Sept. 20, a deputy was dispatched about 8 a.m. to a home on Hammond Creek Trail, Bogart, after a woman said she went to her ex-husband's home to pick up some money. The ex had asked her to come over, but when she arrived he became argumentative. She decided to leave, but he followed her outside and began screaming at her. The man acknowledged they argued but he denied the dispute became physical.
ID THEFT: On Sept. 20, a Wild Azalea Lane, Watkinsville, man reported someone entered his banking account information on a website, then changed his password and ordered some checks that were mailed to a location in Maryland.
BIKE STOLEN: On Sept. 20, a Woodlands Road, Watkinsville, resident reported that someone entered her carport and stole her son's dirt bike valued at $930.
BURGLARY REPORTED: On Sept. 20, a deputy was dispatched about 11 p.m. to Elder Heights Drive, Bishop, for a possible burglary in progress at a vacant house. When the deputy arrived, he saw a car in the driveway with the driver's side door open. Other deputies arrived and they surrounded the house and found a man inside. A man watching over the house for the owner, who was out of town, said the previous resident had been evicted on Sept. 16. The man inside, had a key to the house, and explained he was the ex-husband of the woman who was evicted and he had gone to the house to pick up some of her belongings. The man was issued a prohibitive entry notice and warned he would be arrested if he returned.
HOME ENTERED: On Sept. 21, Deputy Kevin Nolley was dispatched to the club house at Jennings Mill Country Club, where someone broke into the dining area by breaking out glass in a door. The thieves removed six beer tap handles, along with numerous cases of beer.
ITEMS TAKEN: On Sept. 21, a woman, who is the executor of an estate, reported that some items had been removed from a house she oversees on Welbrook Road, Watkinsville.
DOG KILLED: On Sept. 21, Deputy Fredy Wagoner was dispatched to a farm on Old Farmington Road, where a dog had been found dead. The dog, found in a ditch near the backyard of the farmhouse, had apparently been killed about two days earlier. A wound near the neck and one on the chest indicated the dog may have been shot with an arrow, but if so, the suspect, had removed the arrow as none was found in the vicinity. The owner said the dog had gotten out of its kennel. The prize-winning dog, a trained Walker hound used in hunting competitions, was valued at $8,000. The dog was taken for an examination at the University of Georgia to determine the cause of death. Hunters had leased nearby property for deer hunting, but they denied harming the dog.
The Watkinsville Police Department reported the following incidents:
DUI: On Sept. 17, about 2:35 a.m. police saw a Dodge Ram pickup drive onto the shoulder of Whitehall Road, then cross a double yellow line. The pickup was stopped and Robert W. Bagley Jr., 43, of Athens was charged with DUI. During the week, police responded to 29 calls for assistance in the city.
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text:
Lau, Kim S., Robert W. Grange, Eiji Isotani, Ingrid H. Sarelius, Kristine E. Kamm, Paul L. Huang, and James T. Stull. nNOS and eNOS modulate cGMP formation and vascular response in contracting fast-twitch skeletal muscle. Physiol. Genomics 2: 21–27, 2000.—Nitric oxide (NO) from Ca2+-dependent neuronal nitric oxide synthase (nNOS) in skeletal muscle fibers may modulate vascular tone by a cGMP-dependent pathway similar to NO derived from NOS in endothelial cells (eNOS). In isolated fast-twitch extensor digitorum longus (EDL) muscles from control mice, cGMP formation increased ∼166% with electrical stimulation (30 Hz, 15 s). cGMP levels were not altered in slow-twitch soleus muscles. The NOS inhibitor Nω-nitro-l-arginine abolished the contraction-induced increase in cGMP content in EDL muscles, and the NO donor sodium nitroprusside (SNP) increased cGMP content ∼167% in noncontracting EDL muscles. SNP treatment but not electrical stimulation increased cGMP formation in muscles from nNOS−/− mice. cGMP formation in control and stimulated EDL muscles from eNOS−/− mice was less than that obtained with similarly treated muscles from control mice. Arteriolar relaxation in contracting fast-twitch mouse cremaster muscle was attenuated in muscles from mice lacking either nNOS or eNOS. These findings suggest that increases in cGMP and NO-dependent vascular relaxation in contracting fast-twitch skeletal muscle may require both nNOS and eNOS.
- endothelial nitric oxide synthase
- neuronal nitric oxide synthase
- arteriolar relaxation
nitric oxide synthases (NOS) convert l-arginine to l-citrulline and NO in the presence of molecular oxygen and other cofactors (19). Several isoforms of NOS have been identified, including neuronal NOS (nNOS), endothelial NOS (eNOS), and inducible NOS (iNOS). NO generated in endothelial cells initiates a vasodilatory cascade in vascular smooth muscle tissue by activating soluble guanylyl cyclase (sGC) to promote cGMP formation (18). Elevated cGMP activates cGMP-dependent protein kinase (PKG), leading to decreased intracellular calcium concentration ([Ca2+]i) and subsequent relaxation. The resulting vasodilation increases blood flow in the affected vascular bed (18).
For exercise to be sustained, the necessary increase in blood flow to contracting skeletal muscle can be achieved in part by a decrease in skeletal muscle vascular resistance (1). The exercise-induced hyperemic response of skeletal muscle vasculature or active/functional hyperemia is a well-established phenomenon; however, the precise mechanisms that regulate skeletal muscle perfusion during exercise remain incompletely understood. Factors implicated as vasoactive agents to mediate functional hyperemia include low oxygen tension (27), adenosine (28), potassium, pH, magnesium, and osmolarity. Although these factors all have vasodilatory effects in contracting skeletal muscle, their precise contributions to mediating the exercise-induced hyperemia are not well defined.
NO produced by eNOS and nNOS may also contribute to functional hyperemia (12, 16), because NO is formed in contracting skeletal muscle (2). After stimulation of cell surface receptors or mechanical shear stress in contracting muscle, the vascular endothelium produces NO as increased [Ca2+]i leads to activation of Ca2+/calmodulin-dependent eNOS (24). Additionally, fast-twitch skeletal muscle fibers contain nNOS colocalized to the sarcolemma with dystrophin (4, 5), but the contribution of nNOS-derived NO to functional hyperemia is less clear. We considered the possibility that NO produced from Ca2+/calmodulin-dependent nNOS in contracting fast-twitch skeletal muscle fibers could diffuse to adjacent vascular smooth muscle cells to elicit a localized relaxation response via the NO/cGMP-mediated pathway. To this end, we have previously shown that cGMP formation is increased in contracting extensor digitorum longus muscle (EDL) in vitro from C57Bl6 but not mdx mice (16). In Duchenne's muscular dystrophy (DMD) and in dystrophin-deficient mdx mice, both nNOS expression and activity are greatly reduced (5). These reductions may explain both the decrease in cGMP and the attenuated arteriolar dilation observed in contracting muscles from mdx mice compared with C57Bl6 mice (16). In addition, NO produced by nNOS in skeletal muscle antagonizes sympathetic α-adrenergic vasoconstriction during muscle contraction in C57Bl6 but less so in mdx mice (32). This sympatholytic event appears to be fiber-type specific, because it occurs in contracting fast-twitch, glycolytic but not slow-twitch, oxidative muscles (31). Collectively, these findings (16, 31, 32) suggest that nNOS may play a specific functional role in the exercise-induced hyperemic response of contracting fast-twitch skeletal muscle fibers. However, in addition to the decrease in nNOS expression in mdx mice, the possibility that dystrophin-deficient muscles have coexisting derangements that interfere with the NO/cGMP signaling pathway could not be excluded.
In this report, we test the hypothesis that NO produced specifically by nNOS in contracting skeletal muscle stimulates the formation of cGMP and that this increase in cGMP is associated with a hyperemic response. We used fast-twitch (EDL and cremaster) muscles isolated from C57Bl6 mice as well as nNOS-deficient (nNOS−/−) and eNOS-deficient (eNOS−/−) mice to measure both cGMP formation and arteriolar relaxation in response to electrical stimulation. The use of nNOS−/− and eNOS−/− mice has the advantage of allowing assessment of individual contributions of nNOS or eNOS to cGMP formation and arteriolar responsiveness to skeletal muscle contraction. We also evaluated the potential signaling effects of NO on cGMP formation in slow-twitch soleus muscles, which have more eNOS and less nNOS compared with EDL muscles. We demonstrate that cGMP formation in contracting EDL muscles is nNOS dependent, but lack of eNOS also attenuates the response. We also show that the skeletal muscle vasodilatory response to contraction requires both nNOS and eNOS.
C57Bl6 mice (12–15 wk old) were purchased from Jackson Laboratories. The eNOS−/− (10) and nNOS−/− (11) mice were obtained from our colony, originally established with breeder pairs. Chemicals and biochemicals were purchased from Sigma. Antibody to cGMP was obtained from Calbiochem. 125I-cGMP was a kind gift from Dr. David Garbers at UT Southwestern.
Isolation of muscles.
Mouse EDL and soleus muscles were isolated and mounted on Grass FTO3.C force transducers and bathed in physiological salt solution (PSS) containing (in mM) 120.5 NaCl, 4.8 KCl, 1.2 MgSO4, 1.5 CaCl2, 1.2 Na2PO4, 20.4 NaHCO3, 10.0 dextrose, and 1.0 pyruvate at 30°C. The buffer was continuously gassed with 95% O2-5% CO2, resulting in pH 7.6 at room temperature (16). Muscles were either electrically stimulated at 30 Hz for 15 s, incubated with 1 mM Nω-nitro-l-arginine (NLA) for 30 min or with 10 μM sodium nitroprusside (SNP) for 30 s, or subjected to specific combinations. After specific treatments, muscles were quick-frozen by tongs prechilled in liquid nitrogen and stored at −80°C.
Radioimmunoassay of cGMP in muscle extracts.
Briefly, trichloroacetic acid-extracted muscle samples were applied to individual alumina columns, which were then sequentially washed with 10 ml of water, 5 ml of 0.6 N HCl in 95% ethanol, 5 ml of 50% ethanol, and 2 ml of water. Alumina columns were then piggy-backed over Dowex 50W-X8 columns. Three milliliters of 200 mM ammonium formate was added to the alumina columns to elute nucleotides into the Dowex resin. The piggy-backed columns were placed over vials, and 1 ml of 200 mM ammonium formate was added. After the liquid was passed through the alumina resin, cGMP was further eluted from the Dowex 50W-X8 resin with 3 ml of water, and samples were lyophilized overnight. cGMP standards contained 5, 10, 20, 40, 50, 100, and 200 fmol of cGMP. Lyophilized samples and standards were acetylated by redissolving with 25 μl of 4% triethylamine and 25 μl of 2% acetic anhydride, mixed, and placed in an ice bath. 125I-cGMP in 90 mM sodium acetate buffer, pH 4.75, with 20,000 cpm in 50 μl, and 20 μl of the diluted anti-cGMP antibody in 0.1% γ-globulin were added to each tube and mixed. To estimate nonspecific binding, 50 μl of 0.1% γ-globulin was added without anti-cGMP antibody. Samples were incubated overnight at 4°C. Heat-inactivated fetal bovine serum (50 μl) was added to each tube and immediately followed with 1 ml of 12% polyethylene glycol (PEG-8000) in 50 mM sodium acetate buffer, pH 6.2. Tubes were incubated for 1 h on ice and centrifuged (2,500 g, 30 min at 4°C). Immunoprecipitated complexes were washed with 1 ml of 12% PEG, then further incubated on ice for 20 min. After centrifugation, 125I radioactivity in immunoprecipitated complexes was measured in a gamma spectrometer.
Western blot analysis of nNOS and eNOS in muscle extracts.
Mouse skeletal muscles (EDL, soleus, or cremaster) were isolated from C57Bl6, nNOS−/−, and eNOS−/− mice, weighed, and homogenized in 20 vol of 50 mM Tris · HCl, pH 7.5, 1 mM EDTA, 0.1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride, 1 μg/ml of pepstatin A, 2 μg/ml of leupeptin, 1 μg/ml of aprotinin, 1 mM N-α-p-tosyl-l-lysine chloromethyl ketone (TLCK), 2 μM tetrahydrobiopterin, and 20 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). Protein determination of extracts was performed using a Bio-Rad DC Protein Assay kit. nNOS protein in muscle extracts was analyzed by electrophoresis of 20 μg of total protein from each muscle sample on a 6% SDS-polyacrylamide gel. eNOS protein was analyzed by electrophoresis of 60 μg of soluble protein (17,000 g supernatant fraction) from muscle extracts on a 6% SDS-polyacrylamide gel. Resolved proteins were transferred to nitrocellulose paper and subsequently probed for nNOS and eNOS. A positive nNOS marker prepared from mouse brain and an eNOS marker from mouse heart were also included. A polyclonal antibody raised against the NH2 terminus of rat brain nNOS was used (1:4,000) to detect nNOS, which includes μNOS, the alternatively spliced isoform specific to skeletal muscle (29), and a monoclonal eNOS antibody (Transduction Laboratories) was used (1:1,500) to detect eNOS. Horseradish peroxidase conjugated to goat anti-rabbit (nNOS) or anti-mouse (eNOS) IgG was used as secondary antibody. Immunoreactivity was detected by ECL chemiluminescence (Amersham). Relative nNOS and eNOS amounts were quantified by densitometric scanning of immunoblots. For relative comparisons, muscle samples were analyzed on the same immunoblot.
Vascular response measurements in mouse fast-twitch cremaster muscle.
Adult male mice were anesthetized with pentobarbital sodium (70 mg/kg ip), tracheotomized, maintained with supplemental anesthetic as needed via a jugular venous catheter, and kept at body temperature (37°C) by radiant heating. The right cremaster muscle was prepared and the microvasculature viewed as described elsewhere (16); like EDL, cremaster muscle consists predominantly of fast-twitch fibers (unpublished data). The prepared cremaster muscles were superfused with PSS containing (in mM) 131.9 NaCl, 4.7 KCl, 2.0 CaCl2, 1.2 MgSO4, 20 NaHCO3, 1.0 pyruvate, and 10.0 glucose. The buffer was equilibrated with 21% O2-5% CO2, balanced with N2, to give pH 7.4 at 37°C. Electrical field stimulation via silver foil electrodes was used to contract the muscle for 15 s at 30 Hz (0.2 ms duration, 1–3 V). Because flow-dependent mechanisms have been identified as possible contributors to local arteriolar responses, the muscles were clamped across their origins using a miniature arteriolar clamp to produce "no-flow" conditions comparable to those used in isolated EDL or soleus muscles. Following an equilibration period of 20–30 min, baseline arteriolar diameter was measured, and the muscle was then contracted at 30 Hz for 15 s. Arteriolar diameter was measured within 3 s of the completion of the stimulation. The protocol was completed in C57Bl6, nNOS−/−, and eNOS−/− mice, either under control conditions or with 1 mM NLA added to the superfusate. Separate groups of animals were used for each experimental condition. One vessel was selected for observation in each preparation using predetermined criteria to select either a second or third branch distal to the main inflow vessel and to ensure that all were from the same anatomic location in the microvasculature. Arteriolar diameters were measured offline from videotapes using an electronic caliper calibrated against a videotaped stage micrometer. Maximum diameter of each vessel was taken as that produced by the addition of 10−4 M adenosine to the suffusate at the end of the experiment.
cGMP data were analyzed by a two-way ANOVA; simple effects were examined with a one-way ANOVA. Duncan's post hoc test was used to determine differences between means. Changes in arteriolar diameter were compared using a one-way ANOVA and Newman-Keuls post hoc test. For all analyses, differences were considered significant for P < 0.05. Values are means ± SE.
Expression of nNOS and eNOS in mouse muscles.
Figure 1A shows the relative amounts of nNOS in EDL, soleus, and cremaster muscles from control and eNOS−/− mice. No nNOS was detected in muscles from nNOS−/− mice (data not shown). Amounts of nNOS present in muscles from control and eNOS−/− mice, including the higher molecular weight alternatively spliced μ-isoform (29), were not different. Compared with the fast-twitch EDL and cremaster muscles, which had similar amounts of nNOS, the nNOS content of soleus muscle was ∼30% (P < 0.05).
Similar amounts of eNOS were present in muscles isolated from either control or nNOS−/− mice (Fig. 1B). No eNOS was evident in muscles from eNOS−/− mice (data not shown). In contrast to the distribution of nNOS, the highest expression of eNOS was found in slow-twitch soleus muscle. eNOS expression in EDL and cremaster muscles was similar and ∼40% of that found in soleus (P < 0.05). These results show that amounts of nNOS and eNOS are different in mouse fast-twitch and slow-twitch skeletal muscles. They also show that gene ablation of one NOS isoform does not alter expression of the other NOS isoform.
cGMP formation in mouse fast-twitch EDL skeletal muscle.
Under resting conditions, EDL muscles from control mice contained 4.50 ± 0.93 fmol cGMP/mg wet wt tissue (n = 6). Compared with resting values, cGMP levels increased 166% with electrical stimulation (P < 0.05; Fig. 2). The NOS inhibitor NLA did not affect the resting level of cGMP but completely inhibited the increase due to electrical stimulation. Treatment of the muscles with 10 μM SNP significantly increased cGMP content to 167% of resting values (P < 0.05; Fig. 2).
Under resting conditions, cGMP content in EDL muscles from nNOS−/− mice was similar to that of control EDL (4.50 ± 0.59 fmol cGMP/mg wet wt tissue, n = 13). Direct electrical stimulation of nNOS−/− muscles with or without NLA did not significantly increase cGMP production (Fig. 2). However, the muscles were responsive to the NO donor SNP with cGMP levels increasing to 236% of resting values (P < 0.05; Fig. 2). Thus the absence of cGMP formation with stimulation in nNOS−/− EDL did not result from impairment of sGC activity but rather the absence of NO production.
In EDL muscles isolated from eNOS−/− mice, resting levels of cGMP (2.64 ± 0.31 fmol cGMP/mg wet wt tissue, n = 19) were significantly lower than those from control or nNOS−/− mice (P < 0.05). Electrical stimulation did not result in increased cGMP formation in EDL from eNOS−/− mice. The mean absolute cGMP value from stimulated eNOS−/− EDL (3.92 ± 0.38; n = 8) was similar to resting values for C57Bl6 or nNOS−/− muscles. NLA did not significantly alter cGMP content in resting muscles but diminished cGMP content with electrical stimulation (P < 0.05; Fig. 2). The value of cGMP measured in EDL muscles from eNOS−/− mice treated with 10 μM SNP (4.35 ± 0.41; n = 4) was significantly less than the corresponding value from nNOS−/− mice (10.94 ± 1.39; n = 13, P < 0.05). This result raises the possibility that some aspect of the cGMP signaling pathway in EDL muscles from eNOS−/− mice may be compromised.
cGMP formation in mouse slow-twitch soleus muscle.
In resting slow-twitch soleus muscles from control mice, cGMP content was similar to that of EDL muscles (4.46 ± 0.50 fmol cGMP/mg wet wt tissue, n = 10). However, unlike the response of fast-twitch EDL muscles, cGMP formation was not increased in electrically stimulated soleus (Fig. 3). The absence of increased cGMP formation in response to stimulation correlates to the reduced nNOS protein content in these muscles (Fig. 1A). Thus, the contraction-dependent increase in cGMP formation appears to be specific for fast-twitch fibers.
Vascular response to skeletal muscle contraction.
In the three groups of mice, the vessels studied had similar maximal diameters of 35.4 ± 2.2, 33.8 ± 2.0, and 37.7 ± 2.6 μm in control, nNOS−/−, and eNOS−/− mice, respectively. All groups displayed high resting tone. The mean resting diameters without electrical stimulation for muscles from control animals were 8.0 ± 1.5 and 5.5 ± 1.6 μm in the absence and presence of NLA, respectively. The values for nNOS−/− muscles were 4.8 ± 0.8 and 3.5 ± 0.2 μm in the absence and presence of NLA, respectively, and for eNOS−/− muscles, resting diameters were 3.9 ± 0.2 and 4.1 ± 0.2 μm in the absence and presence of NLA, respectively. In control mice (n = 12), muscle contraction induced a significant increase in diameter (12.30 ± 2.05 μm), and the contraction-induced dilation was significantly reduced (2.68 ± 0.90 μm) in the presence of NLA (n = 12). The contraction-induced dilation produced in nNOS−/− muscles (6.29 ± 1.92 μm, n = 12) was significantly less than results obtained with control muscles but greater than control muscles in the presence of NLA. In contrast, results obtained with eNOS−/− muscles in the presence (0.93 ± 0.20 μm, n = 11) or absence (1.27 ± 0.48 μm, n = 11) of NLA were not different from the dilations observed in C57Bl6 mice in the presence of NLA (Fig. 4). In the presence of NLA, stimulation-induced arteriolar dilations were further decreased to 0.27 ± 0.1 μm (n = 12) in the nNOS−/− cremaster muscles.
Functional hyperemia describes the matched increase in blood flow that supports the enhanced metabolism of contracting skeletal muscle. Among the many metabolic factors thought to mediate this response (27, 28), NO may also play a significant role (12, 26). NO is a potent vasodilator, which activates a cGMP-dependent relaxation cascade in vascular smooth muscle (18). Although eNOS is an important source of NO, we considered the possibility that nNOS localized at the sarcolemma of skeletal muscle fibers may also contribute NO to functional hyperemia during contraction. In the present study, we have evaluated the potential contribution of nNOS-derived NO to cGMP formation in contracting muscles in vitro and the potential link to arteriolar dilation in situ.
The significant increase in cGMP formation in electrically stimulated fast-twitch EDL muscles from control mice was totally abolished by the NOS inhibitor NLA, indicating a contraction-dependent activation of NOS (Ref. 16, and present study). In EDL muscles from nNOS−/− mice, cGMP content was not increased by electrical stimulation but was increased significantly by the NO donor SNP; therefore, the absence of cGMP formation with stimulation was due to the lack of nNOS and not an unresponsive sGC. These data support the hypothesis that nNOS activation during contraction is required for an increase in cGMP content.
To further discriminate the contribution of nNOS from that of eNOS in promoting cGMP production in contracting muscles, we employed eNOS−/− mice. Despite the nNOS contents comparable to control, cGMP formation was not significantly increased after stimulation. However, stimulation values were diminished by NLA treatment, indicating partial nNOS activity. Treatment with SNP produced a significant increase in cGMP content compared with the control value; however, the mean absolute cGMP responses of eNOS−/− EDL muscles following either stimulation or SNP treatment were lower compared with similar conditions for C57Bl6 EDL muscles. Moreover, the lower cGMP formation in SNP-treated eNOS−/− compared with nNOS−/− EDL muscles may indicate that the responsiveness of sGC to NO is compromised in fast-twitch muscles of eNOS−/− mice. Collectively, the cGMP data suggest that nNOS is activated during muscle contraction in vitro, but the responsiveness of sGC to NO may depend on the presence of eNOS.
cGMP formation in response to electrical stimulation is specific to fast-twitch and not slow-twitch muscles, because cGMP formation was not increased in stimulated soleus. Slow-twitch soleus muscles contain significantly less nNOS and more eNOS than either fast-twitch EDL or cremaster muscles. The amount of nNOS protein present in skeletal muscle fibers may be a critical determinant of its role in functional hyperemia. This apparent inverse relationship is demonstrated both in the differences between cGMP formation in stimulated EDL compared with soleus in vitro, as well as in the ability of electrically stimulated control vs. mdx hind limb to attenuate α-adrenergic vasoconstriction (sympatholysis) during contraction in situ (32). For example, there is significant sympatholysis in mouse hind limb from control mice, which have fast-twitch muscles that demonstrate abundant nNOS protein, but attenuated sympatholysis from mdx and nNOS−/− mice (32), which have fast-twitch muscles that demonstrate a marked decrease in nNOS content and activity (5).
In agreement with the role of NO in sympatholysis (32), NO contributes a significant component to the dilation in stimulated isolated fast-twitch cremaster muscles. However, the dilation was not completely inhibited by NLA. This result suggests, not surprisingly, that there are both NO-dependent and NO-independent dilatory mechanisms, including metabolic mediators of functional hyperemia (27, 28). The arterioles studied in all three groups of mice were of similar size (maximal diameter), and all displayed considerable resting tone due to the presence of 21% oxygen in the superfusate. Estimated dilatory capacities in these vessels were consequently quite large, being ∼27, 29, and 34 μm in control, nNOS−/−, and eNOS−/− mice, respectively. In stimulated cremaster muscles of control mice, the observed 2.5 times dilation from resting diameter was thus a dilatory response of ∼45% of the maximal dilatory capacity. Although nNOS−/− mice also increased their diameter by a similar 2.3 times, this response constituted approximately only 22% of their dilatory capacity, while in the eNOS−/− group, the response was about 10% of their dilatory capacity. Because we used systematic criteria independent of size to select vessels for observation, the finding that maximal diameters were not different among the groups suggests that there was no significant compensatory change in microvascular anatomy at this level of the microvasculature in the nNOS−/− or eNOS−/− animals. Thus, we cannot attribute differences in arteriolar responses to effects of compensatory differences in the microvascular network.
In stimulated nNOS−/− cremaster muscles, arteriolar diameter increased to a value greater than the NLA-blocked preparation from control mice. This contrasts with the finding in EDL muscle that cGMP did not increase with stimulation in nNOS−/− mice. Assuming that the responsiveness of the cremaster arterioles is similar to the EDL, this prompts several questions: was there an increase in cGMP that we could not detect in our assay due to sensitivity limitations; and, did NO act via a cGMP-independent as well as dependent pathway? In stimulated EDL muscles from nNOS−/− mice, cGMP may have increased to a level which we could not detect, but because of the amplification inherent to signal cascades, this was enough to produce a modest dilation.
It is possible that eNOS in endothelial cells might be activated by the mechanical stress of contraction (e.g., vessel compression) indirectly to produce NO. Other potential activators of eNOS include acetylcholine and adenosine. For example, during stimulation acetylcholine might be released from nerve terminals (14) or from endothelial cells (25), and adenosine may be formed in the interstitium (9). However, cGMP formation was not increased in stimulated nNOS−/− EDL where only eNOS was present, and stimulation was not sufficient to increase cGMP in soleus muscles, despite the presence of significant eNOS protein. In regard to the soleus, we cannot, however, discount that there are distinct differences in the regulation of functional hyperemia relative to fast-twitch muscle.
In C57Bl6 and nNOS−/− mice, a contribution from NO is suggested by the observation that the stimulation-dependent arteriolar dilation was attenuated by NLA. This is in accord with studies on human forearm blood flow during exercise, which showed a significant attenuation of increased forearm blood flow with infusion of a NOS inhibitor during exercise (6). The moderate arteriolar dilation that occurred in response to stimulation in nNOS−/− mouse cremaster may be attributed to both NO-independent and cGMP-independent pathways. Metabolic effectors associated with muscle contraction could account for the NO-independent relaxation (e.g., potassium, pH). However, because dilation was partially attenuated in the stimulated nNOS−/− cremaster by NLA, it is possible that an NO-dependent but cGMP-independent pathway was also activated. A number of studies have reported cGMP-independent vasodilatory responses to NO in several vascular tissues (8, 20). Recently, it was shown that NO blocks the production of 20-hydroxyeicosatetraenoic acid (20-HETE) by inhibiting the enzyme P4504A2. 20-HETE is a potent inhibitor of KCa channels (3), which leads to relaxation by hyperpolarization of smooth muscle cells and decreased [Ca2+]i; thus, inhibition of P4504A2 by NO can result in vasodilation independent of cGMP.
In stimulated eNOS−/− EDL, a modest increase in cGMP was blocked by NLA, yet there was a complete absence of effective dilation in stimulated eNOS−/− cremaster. This result suggests that there was a potential increase in NO with stimulation, but both NO-dependent and -independent relaxation pathways were compromised. nNOS and eNOS appear necessary for the complete contraction-induced NO-dependent arteriolar relaxation seen in control cremaster. This conclusion contrasts with reports indicating compensation by one isoform of NOS in the absence of the other isoform but supports the idea that compensation may depend on the location of the vessel in the circulatory system. For example, an nNOS-dependent mechanism appears to compensate for the absence of eNOS in mounting a vasodilatory response to acetylcholine in pial arterioles in eNOS−/− mice, because the vasodilation is blocked by 7-nitroindazole, an inhibitor of nNOS (21, 22). However, in a separate study with eNOS−/− mice, no nNOS-dependent compensatory relaxation mechanism was observed in carotid arteries treated with acetylcholine (7). In both cases, the sensitivity of carotid arteries and pial arterioles from eNOS−/− mice to SNP was increased (7, 21, 22), but this increased sensitivity was not observed in the eNOS−/− cremaster. Thus, compensatory mechanisms of nNOS or eNOS and related changes in sensitivity of sGC to NO may depend on the type and location of the vessel in the circulation. The attenuated cGMP formation with SNP in the eNOS−/− EDL identifies sGC as a possible candidate protein for decreased amount of activity in eNOS−/− fast-twitch skeletal muscles. Other possibilities in this cGMP signaling cascade should also be considered.
In summary, cGMP formation with muscle activation in vitro is due primarily to NO produced by nNOS and occurs in fast-twitch but not slow-twitch muscles. The cGMP response in fast-twitch muscle is attenuated in the absence of eNOS. In contracting isolated fast-twitch muscles, both nNOS and eNOS appear necessary for arteriolar dilation.
We thank Kimberley Burzynski and Patricia A. Titus for expert technical assistance.
This work was supported by National Heart, Lung, and Blood Institute Grants HL-06296 (to J. T. Stull) and HL-56574 (to I. H. Sarelius) and by a grant from the Muscular Dystrophy Association (to J. T. Stull).
Address for reprint requests and other correspondence: K. S. Lau, Dept. of Physiology, Univ. of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75235-9040 (E-mail: klau{at}mednet.swmed.edu).
Article published online before print. See web site for date of publication (http://physiolgenomics.physiology.org).
- Copyright © 2000 the American Physiological Society


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Well, so far, the results from his urine analysis have come back normal. He does not have any of the metabolic disorders they tested him for.
I still have yet to hear back about the blood work or the kidney ultrasound. I have been trying to get a hold of the doctor to find out when they are going to refer him to a specialist.
i have been off the meds/vitamins/supplements for a few weeks now and actually the smell is clearing up. I am beginning to think that perhaps the smell was a result of something I was taking.
well, other than that, everything seems to be okay...no more long periods of time with out wetting his diapers..
thanks girls!
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Hello and welcome back Blue Hair! I missed you a lot!
Please excuse the vague blog post, I'm writing this as I'm wolfing down a glorious burrito. <3
SUPER glad to have blue hair again. I'm so thankful to have a job that allows mermaid hair! So stoked to dye my hair again all the time!!
By the way, useless thoughts inside my brain RN:
1.) Been obsessed with looking for the perfect nude lipstick. I blame Kylie Jenner and her pretty lips for this, NEED HELP!
2.) Ignoring colored clothes for a while. I blame the blue hair for this.
3.) Bottom ends have turned to mush. So I've been applying hair masks like crazy
4.) PANDAS ON MY SHIRT, Hell yes.
Shirt - Gift from Japan
Skorts & Leather Bagpack c/o Jessica Buurman
Rosary - F21
Sneakers - VANS
XOXO, Marion
, by Forestdoll


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Cold cases could be solved with new fingerprint tool: RCMP
Local Mounties have got their hands on a new $350,000 fingerprint detector far more sensitive than traditional techniques — it can even find prints on clothing and fabric — that holds the potential to solve cold cases.
According to a report from Surrey police Chief Supt. Bill Fordy, the new technology can also detect prints on "exhibits that have been submerged in water, even if they have been submerged for many years."
It's called vacuum metal deposition and works by using tiny metal filings to stick to residue found on fingerprints.
The process, according to the RCMP, is quick — just 15 minutes, typically — and can be photographed immediately.
"This technology is far more sensitive than traditional methods of fingerprint development and as a result has been extremely successful in recovering fingerprints in a number of cold cases where traditional development techniques have failed," Fordy said in his report.
The equipment needs a dedicated room for housing and it's being proposed by Fordy to be placed at the main Surrey detachment. He used one example where a 17-year-old Alberta homicide was recently solved using an older model of the new system.
"This new fingerprint detection technology will assist RCMP and other law enforcement agencies from across the country with identifying suspects in criminal investigations," Fordy said.


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Eight ways to invest sustainably
Households can use 75% less energy when switching to LED lighting from traditional lighting, plus LEDs last up to 25 times longer. Sengled, for example, creates hybrid LED and home electronics bulbs that can reduce your energy intake. Just go to your hardware or home center store. The new bulbs are more expensive, but use much less energy and last longer.


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From Gayley Camp, traverse the Palisade glacier about a half mile and head east (left) up a chute to Glacier Notch and the saddle between Mt. Gayley and Mt. Sill. See main page for details on the approach. Exercise care climbing loose rock to Glacier Notch.
Route Description
Start at the base of Mt. Gayley, north of the saddle between Mt. Gayley and Mt. Sill. Scramble up the Class 3 rocks that appear red/orange and a darker band than the rest of the mountain (hence the name, but it's more like "Follow the Dark Orange Road"), just right of the ridgeline. In the alternative, and to avoid travel across the glacier, find a steep southeast gully on the west face that will join the southwest ridge. This is a short and straightforward climb and most get to the summit and back in under two hours from Glacier Notch.
Bob Burd adds the following "spice" to the route: The ridge itself can be followed directly with some very enjoyable class 4, easy 5 in places. The granite is solid and the large blocks provide ample fun with chimneys, mantles, and stemming, even a little tunnelling.
Essential Gear
Ice axe and crampons recommended for the approach.
Miscellaneous Info
If you have information about this route that doesn't pertain to any of the other sections, please add it here.