text:
Have you ever purchased from PartSelect.com before?
Thank you for helping make our site better.
Part Number WP99002780 replaces: 903095, 99002780.
PartSelect Number PS11747717
Manufacturer Part Number WP99002780
Manufactured by Whirlpool
This lower dishrack roller is less than two inches in diameter. It is made entirely of white plastic. If you find that your dishwasher is making loud noises or the lower rack is getting stuck when you are moving it in or out, it could be that the lower wheel assembly needs to be replaced. The dishrack wheels allow for easy movement of the dishrack in and out of the dishwasher. These rollers tend to wear down at the same speed, so it is recommended to replace both pairs at the same time. Open your dishwasher and remove the bottom rack, locate the locking tab, and rotate until it easily slides out of place. Slide the new wheel over the shaft and locate the locking tab to snap back into place.
This part works with the following brands: Maytag, Amana, Jenn-Air, Admiral, Magic Chef, Crosley.
This part works with the following products: Dishwasher.
This part fixes the following symptoms:
Part Number WP99002780 replaces: 903095, 99002780.
The hardest thing was figuring out that you only need to purchase the latch handle because it only comes as a complete latch assembly, why they even list the latch assembly as a separate part in the parts list stupid. (you get the handle and latch already put together and it cost less than the latch assembly without the handle). Anyway all you need to do is disconect the power from the dishwasher (unless you like to live dangerously like me) take your drill with the correct size torx bit and remove all the screws on the inside door pannel (the top center two screws actually hold the latch assembly in place). Disconect the 4 wires from the old part and reconect them to the new part and reinstall all the torx screws being careful not to strip the plastic threads that they screw into if you are using a drill. Whole process takes less than 5 minutes. I am surprised that Maytag used such a flimsy handle assembly in their dishwashers since they are supposed to have the repair men with the most time on their hands. They probably sell just as many handle assemblies as they do dishwashers that have this particular part in it, probably more since you will probably have to change it 2 or 3 times during the life of the unit.
Help other customers find the most helpful instructions.
Were these instructions helpful?
unwrapped wheels ,pulled out drawer and easily put on new wheels...as we live way out in the boonies it was great to be able to order parts and have them delivered so fast...easy to fix....
It took awhile to figure out how to install the peg clips; I still have my manual, but there is no diagram or picture to show how they are installed (and not on the internet either - not even Amana's site), but I figured it out... so here are the instructions I have created.On the rear part of the lower rack, you will notice that there is a row of larger spaces between the bars; we are only concerned with the 3 farthest right spaces & the 6 bars that create them.Install both clips with the 'flange' that wraps around the bar inside the square; this will position the clamps that hold the clip in place on the outside edge of the bar.Place one clip on the far right bottom bar; place another clip on the left hand side of the 3rd space (4th bar from the bar the other clip was attached to on the right).There are small 'legs' that protrude from the folding tines on one end of each; place the tines so that the 2 large tines on both are on the outside, the 4 straight tines are on the inside of the squares when they are folded down, which will put the little 'legs' down by the far left square.Snap the tines into the small clamps on the ends of the clips, so that the loops on the ends will rotate up into the larger clamps when the straight tines are folded down, which will hold them in place.
The upper arm was held in by a torx screw. The shoulder on the screw held the arm in place. Once removed I simply swapped parts and installed screw. The lower arm was held on by a plastic nut with knurled edges that you can remove by hand. The arm simply lifted off and I swapped arms again. The rollers are held on by a curled plastic lock that grips the wire basket.
Inside the Dishwasher on the bottom of unit, I removed the dish cart. The removed the sprayer arm with an adjustable wrench on the plastic nut on the center top. Then removed the top three screws with a Torx 20 screw driver and removed the plastic cover, then removed the single screw above the black plastic water pump piece and took it off. Then removed the six additional screws that were then displated after the previous cover was exposed. Finally I unscrewed the ivory colored plastic shaft which holds the food processor blade from the metal motor shaft with a adjustable wrench. Upon inspecting the ivory plastic shaft I noticed that the threads were stripped inside it. Which means it didn't turn when the motor would turn and wouldn't drive the water pump.I ordered the replacements and noticed that the replacement for the plastic ivory colored shaft was a metal shaft. It had new pieces as the food processor blade had notiches in new places. The kid had all the replacement parts and a good set of instructions for what to replace and where it would go.Then I assembled the washer as I had taken it apart. And it worked perfectly the first time. It has been repaired and working for a few weeks now without incident.
All I had to do was to snap the parts into place. I also had someone come here to find a leak in my dishwasher. It was in the latch and seal that kept the soap dispenser closed. They wanted to charge me over $50 when the part only cost less than $9 with shipping. I just ordered it and installed it myself. Piece of cake!
Slipped into place the two wheels on lower basket that had gone missing; removed brackets for adjustable section that were broken and easily snapped knew ones into place; connected handle to flatware basket and replaced the old one. Why we didn't do this years ago!
Snapped on new wheels
Stuck new wheel on, time to find and order part on partselect 2 min, time to open package and install part 1 min. Total job 3min.
Orderd the part's from your web site (recived my order in a timley fasion thanks) the parts just sliped on and clipped' basket rolls great. Mike Schaffer
Very easy after watching the video. thanks
A really easy and simple repair that required no tools. The parts were delivered within a matter of days. Completely satisfied with transaction and results.
Pretty much just snapped things into place. The new spray arms solved the problem. The old ones were clogged, and there's no way to clean them from the inside (dumb design, in my opinion). Happy times!
All I had to do was litterally snap the wheels into place..it was so easy and so cheap!
Removed screws. New Part does not come as an assy. Need to order handle. Other sights offer full assy for less.
All brand logos are trademarks of their respective owners.
The PartSelect logo is a Registered Trademark of Atlantic Laundry Centres, Ltd.
Copyright © 1999-2017 , Eldis Group Partnership. All rights reserved.


text:
NDSU contingent shores up the barn at Welk Homestead
by Allan Burke
Photographs by Michael Black
Emmons County Record, Linton, North Dakota, May 1, 2014, pages 1 & 3.
For a man who didn't attend high school, Lawrence Welk surely would have been proud. A contingent of 13 volunteers from North Dakota State University, including a professor, a Ph.D. candidate and 11 undergraduate students, spent the weekend repairing the barn on the farm where he was born and raised.
It's not the same barn where Welk practiced his accordion, but it's been on the farm since the 1930s and is part of the historic site that will be operated by the State Historical Society of North Dakota beginning July 1, 2015. Pioneer Heritage, Inc. and the Tri-County Tourism Alliance will manage it until then.
Leading the team of "service learning students" was Tom Isern, University Distinguished Professor of History at North Dakota State University.
Prof. Isern's students are taking his class, History of the Great Plains 431, this semester, and one of the options is helping with a service project. About half of his class made the trip to Strasburg, and the other half will be volunteering at the historic Hutmacher Farm near Killdeer.
The crew's foreman is Clarence Herz, a Linton native and Ph.D. candidate, and assisting Isern at the Center for Heritage Renewal and driver of the truck that brought lumber from Fargo is Michael Black, 62, an NDSU junior and former resident of Sonoma, Calif., where he had a career in sales and marketing.
Hosting the group were Pioneer Heritage and Tri-County Tourism, and they stayed two nights in the Strasburg School where they had breakfast both mornings in the teachers' lounge. John and Bonnie Wiskus of Lehr brought stew in an antique cast iron kettle on Saturday, and on Sunday NDSU Extension Agent Acacia Stuckle of Linton and her mother, Lourie Jonas, of Wishek served knoepfle soup, borscht, homemade bread from Sue Balcom of Mandan, sausage and dessert by Angie Ibarra.
"We found the barn to need more stabilization than we thought based on earlier visits to the site, so we got a little nervous as we started pulling things apart," Isern said. "Some of the cross beams were no longer attached to the outside walls and were floating free."
They replaced the loft floor with sheets of three-quarter-inch plywood, reinforced the pillars by doubling them with new wood, doubled the lateral beams and shored up the cross beams under the loft decking.
"We have one corner of the floor to finish, and we hope to do that and other work later this summer," Isern said.
Isern said the tentative plan is to organize a work party in July or August after harvesting is completed, and local people as well as volunteers from around the state will be invited to participate as part of the state's 125th anniversary.
"We're thinking about calling it 'Proving Up the Welk Homestead,' and there is talk of having a sausage contest and polka music," Isern said.
He also plans to bring another student group to the site this fall to do cleanup work.
"Once the barn is finished, the rest that needs to be done at the site is mostly cosmetic work," he said.
Isern said he is excited about involving students in historical preservation and other service projects.
"We need to tap the power of 14,000 students to do good things in the state," Isern said.
At the closing lunch at the school, Isern thanked the students for their "good-hearted and talented service" and said he was glad they had the opportunity to experience "German-Russian Country."
The foreman
Clarence Anthony Herz, Jr. is known to his Linton friends and high school classmates (LHS 1985) as Tony but is Clarence at NDSU. His parents are Peggy Herz, who recently retired as the Elementary Principal at New Town, and the late Clarence Herz.
After high school, Herz suffered a broken neck in a car accident while serving in the U.S. Marine Corps and retired from the Marines.
His varied career includes graduating from NDSU with a degree in economics, serving on a crab boat in the Bering Sea, driving a semi-truck and time as an economist in the U.S. Bureau of Labor Statistics in Washington, D.C. He had his own construction company in D.C. before becoming a petroleum landman in the Bakken in western North Dakota.
Herz returned to NDSU in the fall of 2009 and earned his Master's Degree in American History. He is in his first year of a multi-year Ph.D. program in Great Plains History. His Master's thesis is titled, "Petroleum Exploration History in North Dakota to 1951."
"We had a great crew at the Welk Homestead, and they accomplished a lot in a day and a half," Herz said. "I look forward to coming back to my home area to do more work at the site."
He said the back of the barn needs some work, including the replacement of footers. There is also some concrete work to do.
The crew
Members of the crew, in addition to Isern, Herz and Black, are:
- Theresa Daly is from Las Vegas, Nev., and is a sophomore majoring in History.
- Melinda Deugan of Vining, Minn., is a senior studying Music with an Equine Science minor.
- Kayla Haas is a native of Lemmon, S.D., and is a junior with a major in Criminal Justice and Psychology.
- Mitch Herzog of Osakis, Minn., is a sophomore majoring in Crop and Weed Sciences.
- Justin Kiesow of Goodrich, Minn., is a freshman majoring in Agricultural Systems Management.
- Keaton Leymon of Warren, Minn., is studying Natural Resource Management and is a senior.
- David Nigon of LaCrosse, Wis., is a freshman majoring in Mechanical Engineering.
- Senior Duane Powell is from Clearwater, Neb., and is a French major.
- Ethan Rostvedt of Minot is a senior in Criminal Justice.
- Junior Typhanie Schafer is a junior in Public History and is from Audubon, Minn.
Sheets of plywood for the new loft floor are lifted up by hand.
Courtesy of Emmons County Record

subset: commoncrawl


text:
Experts estimate fewer than 50% of card-issuing credit unions will have EMV-equipped credit and debit cards ready by October 2015.
Payment processors say CUs are behind pace to upgrade to EMV chips by October 2015 and may face fraud loss liability.
PULSE survey finds 17% of credit unions said they'll face the risk.
If banks can beat retailers in the race to adopt EMV technology, they may have a fraud liability advantage.
A new report from Aite Group says 70% of credit and debit cards will have EMV chips by December 2015.
The big box retailer will launch the proprietary credit cards June 23.
Chip-and-PIN cards will work at retailer terminals.
The EMV Migration Forum expects another 100 million EMV-enabled cards will be issued over the next year.
Confusion over what chip and PIN technology can and can't do has experts questioning if EMV implementation is a good idea.
Competing choices for chip-and-PIN emerging as liability deadline nears.


text:
Jimmy Jameswrote a review of Skullcandy Fix In-Ear Headphones on December 27, 2011
Overall, pretty cool.
So far so good on these. We'll see how they hold up. High and low end sound great. Bass response is much better than I thought, so that's quite pleasing to my ears. The only thing I don't really like about these is that the volume is adjusted by pressing down/clicking on the control as opposed to having a slider control and the mic/volume control is not compatable with my Droid phone so it's pretty much functionless with my cell. Worked just fine on my five mile run yesterday and the volume/search remote worked well with my Ipod Nano. They do stay pretty much fixed in my ears, but they aren't like superglue...they will still move if bumped just right or the cord snags on something. That's why a guy always tucks in the cord yes?
They aren't noise cancelling phones, but they pretty much block out all extraneous noise once you put on the correct size gel bud covers. The cord length is a little longer than my Sony buds, but I like that. Comes with a sweet case, but it will only hold my new Fix buds, not big enough to hold my Nano as well...who needs a case for buds only? Not me. These are sweet enought that I'm going to look into another set for my wife.


text:
The 14-mile stretch of the new Interstate 495 Express Lanes are on track to be open by the end of the year.
And on Thursday, VDOT officials gave an update on the look of the Express Lanes, which are 90 percent completed, and how they will operate.
There will be no gates on the lanes, which go from Springfield to the Dulles Toll Road. Instead, E-ZPass transponders will calculate tolls on the road as you enter and exit. Those tolls will vary depending on how far drivers go.
Starting this summer, those who carpool will be able to turn in their current E-ZPass transponders for a new E-ZPass Flex transponder. That will allow drivers to use the transponder in both HOV-3 mode and non-HOV mode with the flip of a switch. HOV-3 qualifies for toll-free travel.
Officials estimated that by using the Express Lanes, commutes from Springfield to Tysons Corner would be cut in half. However, officials believe that most users will only take the new road for about three miles. They said most people are not expected to use it for a daily commute, since that three-mile trip could cost $5 or $6. Sensors will monitor traffic 24 hours a day and the system will adjust toll prices as necessary.
In order to patrol the road and enforce HOV restrictions, 11 state troopers will be hired, with a private contractor footing the bill. The Express Lanes themselves are a public-private partnership between VDOT, the Virginia Department of Rail and Public Transportation, Federal Highway Administration and Transurban-Fluor.
For more information on the new road, visit 495ExpressLanes.com.


text:
Oklahoma was able to have first-half success moving the ball on the Texas defense through the air last week. Defensive backs coach Duane Akina said the task this week against Oklahoma State will be...
Premium Content
Subscribe today to read the full article and get everything Rivals has to offer.
- Member-only message boards
- Exclusive highlights and recruiting interviews
- Exclusive coverage of Rivals Camp Series
- Breaking recruiting news


text:
Maserati has finally pulled the wraps of the Ghibli, its new smaller sedan that will be fully revealed later this month at the Shanghai Motor Show. The Ghibli is positioned as a "sports executive" sedan that will take on the likes of the Audi A6, BMW 5 Series, and Mercedes-Benz E-Class.
As our earlier spy photos showed, the Maserati Ghibli looks like a scaled-down version of the new Quattroporte. A wide grille with vertical slats dominates the car's nose, the slopes down dramatically into squinty headlights, and the fenders rise dramatically over the front wheels. Each front fender sports a trio of small vents; the car's tail has a stubby trunk with curved character lines that follow from the rear fenders. The interior of the Maserati Ghibli is highlighted by a large touchscreen on the center stack, topped by an analog clock, as well as red leather dashboard accents, a chunky shifter for the automatic transmission, a three-spoke steering wheel, and a simple two-binnacle instrument cluster with a central LCD display.
Maserati says the Ghibli will offer two gasoline turbocharged 3.0-liter V-6 engines, and a 3.0-liter turbo-diesel V-6. An eight-speed automatic is standard, and buyers get a choice between rear- and "Q4" all-wheel drive. The Ghibli is the first-ever Maserati production car with a diesel engine. There is a chance the Quattroporte's 3.8-liter twin-turbocharged V-8 could join the Ghibli range at a later date.
The company claims the Ghibli will attract, "premium executive car buyers [who] are looking to make an individual statement through a car that is distinctive, elegant and luxurious." More seriously, the car is intended to steal sales from the German luxury brands.
The Ghibli is a key part of Maserati's bold plan to expand its range, broaden its appeal, and ramp annual sales up to 50,000 units globally by 2015. In 2012, Maserati sold 6307 cars worldwide.
The Maserati Ghibli will make its public debut later this month at the Shanghai Motor Show in China.
Source: Maserati


text:
So can we just pretend that it hasn't been FOREVER since I've made something with bacon? Great, thanks! Moving on. I'm sure everyone is familiar with the concept of the Elvis sandwich: pb, banana and bacon. What a stellar combo. So I thought I'd do my own take on the King's favorite food as a cookie. As per usual, Alton Brown's The Chewy recipe is my go-to basic dough, but then I added in the extra goodness.
Elvis Cookies
For the full recipe that will have you "all shook up"...yes, I went there, visit Foodbeast.


text:
2017 Wisin Y Yandel Tickets
|Jan 20, 2018
Sat 8:00PM
|Calibash: Jennifer Lopez, J Balvin, Ozuna & Bad Bunny
Staples Center - Los Angeles, CA
If Anyone is searching for Wisin Y Yandel tickets 2017 then www.FindTicketsFast.com is your only online source. FindTicketsFast has Wisin Y Yandel Concert tickets 2017 for any day or concert performance nationwide , and also in Canada. FindTicketsFast.com has one of the most elaborate group of tickets for all the Wisin Y Yandel 2017 tour dates for any time and place. Our Wisin Y Yandel tour 2017 tickets can be organized via sector, time, dollar amount, including quantity. Or if you want support locating the particular tickets you desire FindTicketsFast client service staff can sort through the bulky catalog of tickets FindTicketsFast has collected for you. Our company also can give you explicit facts about Wisin Y Yandel tickets 2017 , in addition to a explicit listing of the Wisin Y Yandel 2017 tour dates.
After you pick out your Wisin Y Yandel 2017 tickets and you are ready to purchase, please don't forget to discover their whereabouts using www.FindTicketsFast.com's color coordinated stadium maps. Each of Find Tickets, LLC's maps exactly illustrates the seat location for each arena where the concert is being put on. If you are having a hard time locating Wisin Y Yandel Tickets 2017 , then feel free to touch base our consumer help dept. our employees' will be eagar to help you with discovering the seats that you desire to book on the Map. While you have a representative on the phone please don't forget to ask The representative about Wisin Y Yandel Concert tickets 2017 as well as any exact concern you should have about the Wisin Y Yandel 2017 tour dates schedule. A customer service rep will be able to assist you in answering whatever that you might not know about the Wisin Y Yandel tour dates 2017 .
After all the hunting you have done to select the seats you want and verifying the seats location on the arena maps that we provide you should be prepared to secure your Wisin Y Yandel Tickets 2017 . www.FindTicketsFast.com urges our patrons to secure the tickets fast once they decide on the Wisin Y Yandel Concert Tickets 2017 they desire. Sometimes while our clients are still deciding because Wisin Y Yandel 2017 tour dates are so popular that their tickets end up being sold to someone else while they make their choice. So Once you make your pick to purchase precise Wisin Y Yandel 2017 Tickets, please phone our guest services department to submit your order. You can also fill in your order via the internet using our secure online ordering system. As with buying anything please check that the Wisin Y Yandel tickets 2017 that you are buying are the ones that you want. Make certain that it is the precise Wisin Y Yandel tour dates 2017 schedule. Compare your ticket order before you hit the buy button on the website with the Wisin Y Yandel 2017 tour dates that we have on our website. It has to match up perfectly with the tickets that you picked with the Wisin Y Yandel tour dates 2017 that you want to attend.
- Wisin Y Yandel Ordering Information -
- Wisin Y Yandel Schedules Information -
- For More Concert Tickets And Events -
Thank you for the great experience. We truly enjoyed the show. Thank you for making it all happen.
Great customer service + excellant prcing = very happy customer!


text:
Uzbekistan, Tashkent, Feb.29 / Trend, D. Azizov /
President of Uzbekistan Islam Karimov received a member of parliament, British Secretary of State for Defence Philip Hammond on Tuesday, who arrived in Tashkent on an official visit on Monday, Uzbekistan National News Agency (UzA) reported.
President Karimov stressed that this meeting provides a good opportunity for extensive exchange of views on the state of the Uzbek-British relations, further cooperation priorities and assessment of the situation in the region.
A pragmatic approach to building bilateral relations allows outlining broad prospects for deepening and enhancing dialogue, strengthening mutual understanding and thereby creating a safe environment and conditions for cooperation development.
During the conversation, the sides discussed issues of regional security and stability, settlement of the situation in Afghanistan, as well as opportunities for development of bilateral military and military-technical cooperation.
At present, Uzbekistan and the United Kingdom cooperate in the international arena, inter-parliamentary contacts, consultations of the foreign ministries, joint work at the level of ministries and departments became regular.
The UK is one of the most important economic partners of Uzbekistan in Europe. Some 419 enterprises with the participation of the British capital successfully operate in the country, also representatives of 95 companies are accredited to the country. Westminster International University in Tashkent plays an important role in strengthening the human resources of Uzbekistan. Together with the University of Cambridge work is carried out to create the Centre of High Technology in Tashkent.
According to official Uzbek media outlets, British Secretary of State for Defence held talks in Uzbek Defense Ministry, where the current state and prospects of bilateral military and military-technical cooperation were discussed. Both sides also exchanged views on the existing military-political situation in the region.
Hammond stressed the positive dynamics of development of bilateral relations with the Defense Ministry of Uzbekistan, as well as the British side's commitment to their gradual expansion on a wide range of issues.
During the visit, the UK delegation visited the Senate of Oliy Majlis (upper house of parliament) of Uzbekistan, where it held a meeting with members of the Foreign Affairs Committee.
Visitors from the UK visited the Independence Square and laid flowers at the Monument of Independence and Humanism.
Do you have any feedback? Contact our journalist at firstname.lastname@example.com
Follow us on Twitter @TRENDNewsAgency
Follow Trend on Telegram. Only most interesting and important news


text:
Nu Online News Service, Nov. 9, 2:57 p.m. EST
Thailand flooding that has forced evacuation of 650,000 people, killed more than 500 and destroyed homes has shut down manufacturing plants that provide supplies to companies in the United States, particularly in the automobile and computer industries, according to AIR Worldwide.
Two of the world's largest car manufacturers, Honda and Toyota, have had severe disruption to their manufacturing processes, not just in Thailand but worldwide due to a lack of parts.
Thailand is responsible for the manufacturing of about a quarter of all hard disk drives produced for the global-computing industry. This round of flooding has raised fears of global supply shortages of hard disk drives for the lucrative Christmas season, AIR notes. Already prices for hard drives are reported to have increased by up to 180 percent.
According to the modeling company, the recent flooding is being described as among the worst in Thailand's history, with some 6 million hectares (more than 14 million acres) of land affected. In the north and central plains, the damage has been predominantly to residential properties and to agriculture.
Flooding was at first confined to the north and northeast of the country but has steadily moved further into the central plains.
As of Nov. 7, 12 of the 50 districts of the capital were on the evacuation list with a further seven sub-districts on partial notice, AIR says. The commercial heart of the city, however, remains dry at present. Officials estimate that flood conditions in the Bangkok area are likely to continue for several more weeks.


text:
Plugins Tab in the About Dialog Box: This screen capture shows the Plugins tab of the About dialog box. The tab contains a table with four columns. The column headings are Name, Version, Status, and Description. In the Name column are the names of the plug-ins, which are the following:
Cube Viewer Plug-in
Level Viewer Plug-in
Measure Viewer Plug-in
Delete Dimension Plug-in
Edit Dimension Plug-in
View XML Plug-in
The version of each plug-in is 1.0, except for the Edit Dimension Plug-in, for which the version number is 2.0. The status of each plug-in is loaded. The Description column contains the following:
Displays the name of a cube.
Displays the name of a level.
Displays the name of a measure.
Deletes a dimension in the MyDims folder.
Edits the short description of a dimension.
Displays the XML for an OLAP measure.


text:
2017 Celebration of Aging
Our 2017 Celebration of Aging was Thursday, March 30 at the Convention Center with Jack Hanna.
"I have loved animals since I was a young boy growing up in Tennessee. I could never have imagined where my life would take me – and it's only getting wilder! My hope is that together we can appreciate, value, and protect wildlife for generations to come."
Use the link below to register and pay securely online.
Submit a nomination for the Eli Rudin Second Half Hero Award.
Click here to register and make a secure online payment.
Submit a nomination for the Carter Catlett Williams Award for Excellence in Aging or Long-term Care Services
We thank our generous sponsors of the 2017 Celebration of Aging.
SuperHero Sponsor
Hero Sponsors
Laborers' Local 435/LECET
Rochester Business Journal
Role Model Sponsors
EFPR Group, LLP and RHCC – Healthcare Consulting
Legacy Senior Living Communities
Rochester Regional Health
Take It On! Sponsors
Ames Amzalak Memorial Trust/Bond Schoeneck & King PLLC/Jay R. Gelb & Company, P.C.
The Bonadio Group
Doyle Security Systems Medical Monitoring
Harter Secrest & Emery LLP
Health Direct Pharmacy
Highland Hospital
Jewish Senior Life
Omnicare, a CVS Health Company
Ontario Center for Rehabilitation and Healthcare
Seneca Park Zoo Society
Touching Hearts At Home
Woods Oviatt Gilman LLP
Circle of Friends Sponsors
Alzheimer's Association
Atria Senior Living
Bond Financial Network
Boylan Code LLP
Brookdale Senior Living
Christopher Communications
CMI Communications
Crimson Ridge Senior Living
Episcopal SeniorLife Communities
Fairport Savings Bank
Friendly Senior Living
Genesee Health Facilities Association
HCR Home Care
Heather Heights of Pittsford
Heritage Christian Home Care
Holy Sepulchre Cemetery & Ascension Garden
Law Offices of Pullano & Farrow PLLC
Legacy Financial Planning
Lifetime Care Home Health and Hospice
Manning & Napier Benefits
Mason Marketing
Panther Graphics
PeerPlace Networks
Pittsford Insurance Agency, LLC
Ron and Kelly Ricotta
Rochester Management, Inc
Rochester Mobile X-Ray
Rochester Regional Joint Board
Special Care Systems
Spectrum Sports
St. Ann's Community
Thomson Reuters
Tipping Point Communications
Tompkins Financial Advisors
Toshiba Business Solutions
Underberg & Kessler LLP
University of Rochester Medical Center
USA Payroll
YourCare Health Plan, A Monroe Plan Company


text:
The Value of Embedded Real Options: Evidence from Consumer Automobile Lease Contracts
- Abstract
- Full Text PDF
- Full Text HTML
- Author(s): CARMELO GIACCOTTO, GERSON M. GOLDBERG, SHANTARAM P. HEGDE
- Published: Jan 11, 2007
- Pages: 411-445
- DOI: 10.1111/j.1540-6261.2007.01211.x
Under the common assumption of constant interest rates, we show that penalties for early termination of a lease are often structured in such a way that the cancellation option embedded in consumer automotive leases has little value. Furthermore, our estimates drawn from a sample of three popular car models over 1990 to 2000 indicate that the stand‐alone value of the lease‐end purchase option is, on average, about 16% of the market value of underlying used vehicles, or about $1,462 per contract. Finally, we examine the sensitivity of our option value estimates to model parameters and default risk.


text:
Landmark Renovation of Macy's Herald Square Debuts the Herald Square Cafe on 2
Exclusive coffee, champagne and chocolate to be served and savored while shopping The World's Largest Shoe Floor
Sep 04, 2012, 12:00 ET from Macy's
NEW YORK, Sept. 4, 2012 /PRNewswire/ -- As part of its landmark multi-year, 400 million dollar renovation of Macy's Herald Square, the iconic retailer debuts the Herald Square Cafe this week, just in time for the fall fashion season. Nestled within The World's Largest Shoe Floor on 2, the Herald Square Cafe is a welcome addition to New York's newest and premier shoe shopping destination. Tempting all five senses with exotic coffees, artisan chocolates and fine champagne, the Herald Square Cafe offers shoppers the ultimate trifecta of indulgence after browsing the "must-shop" destination of the fall 2012 season.
Providing the ideal shopping respite, the Herald Square Cafe will offer coffee connoisseurs an elevated coffee experience with rare varieties of Starbucks Reserve™ coffees brewed using the innovative Clover® brewing system, as well as Starbucks signature espresso beverages. In addition, a special selection of champagne from the celebrated cellars of Moet & Chandon will be available, as well as exquisite hand-crafted chocolates by Vosges Haut-Chocolat. A special assortment of savory and sweet menu items, along with a selection of red and white wines by the glass will round out the experience.
Visitors to the intimate 45 seat, 1,434 square foot custom-designed cafe will step into a sleek and chic environment of heritage marble with modern wood accents and dramatic lighting bringing a touch of warmth to the space. Curved banquettes upholstered in cream and gun-metal gray elegantly define the perimeter of the dining space providing inviting seating vignettes. A grand clock serves as a striking focal point overlooking the communal "champagne tasting table." This clock has been fashioned in the mirror image of the original timepiece which overlooks Broadway Boulevard and has been keeping time for busy New Yorkers since the filming of "Miracle on 34th Street." Natural light will illuminate the second floor space through the majestic historic windows which have been renovated and made visible to the public for the first time in decades. The cafe will operate during normal store hours.
According to Patti Lee, District Vice President, General Manager for Macy's Herald Square, "Our goal in developing The World's Largest Shoe Floor was to create the ultimate shoe shopping experience for the Macy's customer. The Herald Square Cafe plays an integral role in this vision, elevating the shopping experience and resulting in a virtual wonderland of indulgence for our customers with amenities never before offered in the same setting. An incomparable assortment of the season's hottest shoes, combined with the finest chocolates, coffee and champagne, all within arm's reach--is there a shopping experience that is more indulgent, luxurious and fantasy-filled than that?"
About Macy's
Macy's, the largest retail brand of Macy's, Inc., delivers fashion and affordable luxury to customers at more than 800 locations in 45 states, the District of Columbia, Puerto Rico and Guam. Macy's stores and macys.com offer distinctive assortments including the most desired family of exclusive and fashion brands for him, her and home.
About Macy's Herald Square
Macy's Herald Square is New York City's most iconic department store, welcoming more than 20 million visitors from around the world each year. The world-famous flagship is a retail institution known for its fashion and epic events -- most notably the Annual Macy's Flower Show®; Macy's 4th of July Fireworks® and the Macy's Thanksgiving Day Parade®. Built in 1902, the "World's Largest Store" currently houses more than 1 million square-feet of retail space. In 2011, Macy's Inc. announced that the store will increase its selling space to 1.2 million square-feet following an extensive four-year, 400 million dollar renovation. The renovation will transform the store into one of the most technologically advanced, fashion-forward and exciting destinations in the world with new designer departments, epicurean experiences and multi-level luxury shops.
For Macy's media materials, images and contacts, please visit our online pressroom at www.macys.com/pressroom.
SOURCE Macy's


text:
Sushi comes in many forms—the grab n' go package, the dodgy half price buffet and, of course, the classy over-priced restaurant with the open kitchen so you can admire the chef's handy work. Out of my experience in all three types of establishments, I always come away with utter admiration for the sushi chef. Even […]Continue reading
'Tis the Season: Restaurant Week Summer 2010
I feel like Restaurant Week is an unofficial holiday in New York City. This bi-annual event unlocks the door to restaurants otherwise reserved for bankers and ballers, and instead lets the average, aspiring foodie enjoy an affordable $25 lunch or $35 dinner. Restaurant Week(s) is actually a period of two weeks, organized by NYC & […]Continue reading
New Location, Same Great Margs
Mmmm margarita season is in full swing and luckily my favorite margarita eatery,Cowgirl Seahorse, just opened a second location on the South Street Seaport. Awhile ago I wrote a post detailing my unyielding love for Cowgirl's margaritas, a love that is luckily shared among my friends! Last Friday I sounded the sirens and we met at Cowgirl […]Continue reading
Green Food Delivery Initiatives Spark Utensil Shortage
Ah, the joys of NYC food delivery. One of the luxuries of living in this fine town is that any cuisine is just a couple mouse clicks and less than an hour away from delivery to your doorstep. The website SeamlessWeb, which aggregates all the restaurants that deliver to your particular building for online ordering, […]Continue reading
Chinatown Night Out
I often forget about Chinatown. Not because it is easy to miss, but rather because I subconsciously avoid it the way most New Yorkers avoid the Times Square hustle. Daytime Chinatown calls to mind a crowded mess of tourists shopping for I Heart NY t-shirts and fake Ray Bans and being followed by some man […]Continue reading
Grocery Shopping Relief with FreshDirect
Grocery shopping in NYC is not what I would classify as a fun experience. The Midwest grocery stores of my youth are roughly the size of one city block in this town, which meant I had to adjust to smaller stores, a reduced selection and sharpen my bumper cart skills. The store closest to my […]Continue reading
Best Margs in Town
On St. Patrick's Day most people opt for Guinness or Jameson, but in the interest of avoiding the parade and not replicating Hobo St. Paddy's Day, my friends and I went out for margaritas. As a result, I have crowned my new favorite margarita! This is no small task. New Yorkers pride themselves on where […]Continue reading


text:
Spring is in full force here in sunny SoCal, but that doesn't mean we're ready to give up on donning our black apparel. While everyone is all about their spring whites and pastel colors, we're staying true to our #1 color and making sure it doesn't end up at the backs of our closets. Need help being convinced that black is still totally appropriate for spring? Here are a few reasons why black is necessary ALL YEAR ROUND:
1. It's slimming. Black looks great on everyone and there's no denying the slimming power it has as soon as you slip it on. Some kind of wonderful, wonderful sorcery.
2. It's sexy. The perfect LBD will help you achieve that mysterious and alluring look.
3. It's a great canvas for accessories. An all black 'fit is just what your colorful pops of accessories needs.
4. It's transitional. Black in the day is great and then somehow it transforms into something even more awesome at night!
5. It's classic. Let's not forget how perfect black attire is for any and all spring occasions. Whenever you're in a rush and in need of a classy 'fit for an event, black will be there to save the day.
So let's not turn our backs on black. There's just so many reasons to love it.


text:
NOTE: Neither Vita includes a memory card in the listed price, but you basically need one. Vita prices are a lower to compensate.
1 x 8GB Memory Card - $25 shipped, or $20 included with any Vita console!
1 x 16GB Memory Card - $45 shipped, or $30 included with any Vita console!
Japanese launch unit Vita: $175 shipped. PSVita is REGION FREE, works with US PSN accounts, has English language, all that hot stuff. It's basically a US Vita. Includes the box, manuals, and charger.
US Vita: $175 shipped. Has a very tiny abrasion on the top portion of both shoulder triggers, not very noticeable, caused by using the PDP trigger grips. Couldn't get a good picture of it, sorry. Box, manuals, and charger included.
Deathsmiles (360): $20 shipped. Case and manual included, no soundtrack.
Paypal address is email@example.com. Reply here or send a PM with questions! All items ship from the Florida panhandle, shipping to the continental US only (unless you really want to pay for some high shipping costs).
Edited by Miyomei, 27 November 2012 - 03:39 AM.


text:
Habitat GO Chapters are made up of a group of passionate, engaged volunteers committed to Habitat for Humanity's goal of providing safe, decent and affordable housing for families in need. Chapters are in charge of fundraising for the build, leading construction and finding and supporting families for Habitat homes.
We currently have Chapters located in Carleton Place and Perth.
Interested in volunteering?
The Carleton Place Chapter is currently looking for volunteers to assist with fundraising for their next build.
The Perth Chapter is currently looking for volunteers with build experience, particularly those with a background in construction management to oversee the remainder of the build starting May 2017.
Both Chapters always welcome local volunteers interested in continuing to develop the Habitat Chapter in these communities. Join a Chapter if you have one or more of the following skills or experience in:
- Team leadership
- Volunteer coordination
- Construction
- Land acquisition
- Accounting or finance
- Project management
- Communications and marketing
- Fundraising
- Administration
- Social Work
Volunteers who may not have the above skills, but possess enthusiasm and a willingness to learn can also join!
If you're interested in becoming a committee member for either the Carleton Place or Perth Chapter please email:
Start a Habitat GO Chapter in your community
Habitat for Humanity Greater Ottawa's territory extends west to Petawawa and east to Hawkesbury.
In order to become a Habitat Greater Ottawa Chapter, communities must demonstrate their interest by showing support from community institutions such as town council, chamber of commerce, and also by establishing a volunteer commitment with strong leadership.
Please click here to read Habitat GO's Chapter Manual.


text:
Professional use of social media refers to the communication practices you engage in in an official capacity, posting content as an administrator of a page or in a similar institutional capacity. See best practices.
- Be Yourself – Make clear that you are an employee of UALR and never try to pretend otherwise in order to promote UALR's interests. Users expect transparency, so be upfront about your role. Remember, however, that you are not an official spokesperson for the university. That capacity belongs to the professionals in the Office of Communications and the Department of Digital Strategy.
- Show affiliation – If you've been authorized to create a social web presence for your department, office, or organization, be consistent with the university branding (logos, images), and include links back to the ualr.edu domain.
- Respect others – Always take the high road and be thoughtful in your response to negative feedback. Many times, it's better to let the community come to your defense rather than engaging in an unproductive back and forth. Resist the urge to delete criticism unless it is clearly offensive.
- Use a friendly voice – The institutional voice can sometimes be interpreted as cold and unfeeling. Be casual and don't take yourself too seriously. Through social media, you have the opportunity for a human voice to come through.
- Respond quickly – If someone posts a question, comment, or sends you a tweet, provide an answer as soon as possible. There is an increased expectation of instant gratification on social networks, so check in often.
- Be credible – Part of being authentic means you'll make mistakes. When you do, don't remove the post, simply make the correction. Your followers will appreciate your honesty and your humanity.
- Maintain confidentiality – Do not disclose confidential information about UALR, students, or employees. If you describe a situation, use good judgment and make sure the individuals cannot be identified. Avoid sharing identification information (SSN or T numbers), passwords, or other personal data through social networks.
These guidelines assume compliance with branding standards set forth by the Office of Communications, the Information Technology Acceptable Use Policy established by Information Technology Services, and any other existing or future UALR policies regarding the use of technology, computers, internet, and electronic communications.
The best practices for personal use can also be helpful.


text:
Ali Yakubov, a nine-month-old baby boy from Russia left doctors stupefied after the word Allah allegedly showed up on his chin when he was only a few weeks old.
Since then, verses from the Koran have emerged consistently on the infantâs back, arms, legs and stomach, before apparently fading away and being replaced with new ones.
Ali was born in an ordinary family with no major inclination towards religion. His parents initially concealed the fact about the strange writings but when a phrase âShow these signs to peopleâ appeared the news spread all around the province of Dagestan.
Amen. I had a godly, transparent pastor tell me once that preaching is the easy part and that vigilant prayer is the difficult part.
Two different people, who were being bothered by evil images at night,asked me to pray and they both said that when I prayed the evil images left immediately. There is no magic or verbally claiming the blood, etc., simply by using His powerful Word and having faith in it's promises. One verse that I mentioned was: "Greater is He who is in you, than he who is in the world."
Sometimes we react like the jews and greeks who became fearful after hearing about the evil spirit episode with the sons of Sceva.
God's full armor consists of truth, righteousness, the gospel, faith, the Word of God, and of course, praying at all times in the Spirit.
Lord, please help us to walk in your laws and not to forget your deeds for usso that we do not become cowardly and instead to remember that we have all that we need.
"The sons of Ephraim were archers equipped with bows, Yet they turned back in the day of battle." PSALMS 78:9
As the man said, "Bunkum". Or plain B.S. Yes Satan is real and can play tricks, some pretty impressive ones. But this one here is a real cheap B-grade attempt by Ali's uncle & friends. Especially when the writings exhort âShow these signs to peopleâ. Yeah right!
I think Allgogs pumpkin stands a better chance on, "..the unusual conditions that appear mysterious, perplexing medical experts and defying all logic."
p.s.On the other hand, Ali's lucky that these weren't Mohammad cartoons that his friends painted. WHEW!!!! Safe.
Tony Cisnerros, where are you
Goggles appear mysteriously on yellow skin
Mike wrote:I be he likes cheese sandwiches, too.
Bruce Lantto wrote:satan at work again!!


text:
Expert: Wild animals should not be pets
There's a reason they're called "wild animals" — they were born in the wild and should stay there and people should not try to make them pets or the consequences can be deadly, said an animal behavior expert at Texas A&M University.
Dr. Bonnie Beaver, a former president of the American Veterinary Medical Association and a professor in the College of Veterinary Medicine & Biomedical Sciences, contends that exotic animals, such as lions, tigers, cougars, bears and chimps are best left to be cared for by zoos, not individuals hoping to make them pets.
"Wild animals do not adapt well outside of their native habitat," she said.
"They tend to retain their basic characteristics and their wild nature, and people who try to make pets out of them often get hurt, some very badly. I would strongly urge anyone considering buying an exotic animal to think twice about the idea. There is not one good reason to own one."
Beaver added that there are some amazing stories of individuals who have owned a wild animal for many years, and for no reason at all, the animal turns on them and attacks, sometimes with fatal consequences.
"Something usually triggers the aggressive response in the animal. Either it didn't get what it wanted, or it perceives something or someone as a threat, or it even has a medical condition that is causing it pain — any of these can make the animal turn on its owner quickly and violently.
Another big reason not to own such animals: cost.
The animals often have hefty price tags, some of them costing thousands of dollars, and they often need a special diet that can be very expensive.
Beaver said that even animals that have been declawed or have had their teeth filed down can still cause significant damage to a human. And because exotic animals are often kept outdoors, it means they must be placed in cages, and very often there are numerous problems about such living arrangements, she notes.


text:
Gastroenterology Research and Practice
Volume 2013 (2013), Article ID 130927, 8 pages
ppENK Gene Methylation Status in the Development of Pancreatic Carcinoma
1Department of Gastroenterology, Beijing Chaoyang Hospital, Capital University of Medical Sciences, Beijing 100020, China
2Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, China
Received 11 February 2013; Revised 17 April 2013; Accepted 13 May 2013
Academic Editor: Muhammad Wasif Saif
Copyright © 2013 Lixin Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objective. To explore the association of hypermethylation of the proenkephalin gene (ppENK) with pancreatic carcinoma and to identify the effects of a demethylating agent on pancreatic cell lines. Method. Human pancreatic cancer tissues and five pancreatic carcinoma cell lines, as well as normal pancreatic tissue, were used. ppENK methylation status was detected by MS-PCR (methylation-specific PCR). Results. Methylation of ppENK was detected in 90.3% (28/31) of the human pancreatic carcinoma tissues but was not seen in normal pancreatic tissue. There was no correlation between the extent of methylation of ppENK and the clinicopathological features of the pancreatic carcinomas. Methylated ppENK was detected in all the pancreatic cancer cell lines and was associated with loss of mRNA expression in the pancreatic carcinoma cell lines and normal pancreatic tissue. After treatment with 5-aza-dC, methylated ppENK was not detected and the inhibition of ppENK mRNA expression was reversed. Conclusions. Inhibition of ppENK expression by a change in its methylation status plays an important role in pancreatic carcinogenesis. ppENK methylation is thus an important molecular event that distinguishes pancreatic carcinoma tissue from normal pancreatic tissue. Effects on cell growth, apoptosis, and the cell cycle may contribute to changes of ppENK methylation status.
1. Introduction
Pancreatic carcinoma is highly malignant and has a poor prognosis. The overall 5-year survival rate for pancreatic cancer does not exceed 5% in most studies, because it is generally diagnosed too late to allow surgery, which is still considered a curative treatment for pancreatic cancer. The incidence of pancreatic cancer has steadily increased in recent years. Its pathogenesis is not understood, and novel treatments are urgently needed [1].
The human ppENK gene has been localized to chromosome 8, at q23-q24, and consists of four transcribed exons and three introns. It is a neuropeptide transmitter gene and encodes Met-enkephalin, which is a topically active inhibitor of pancreatic cancers that interacts with the opioid growth factor receptor [2, 3].
DNA methylation is an important regulator of gene transcription, and its role in carcinogenesis has been a topic of considerable interest in the last few years. Alterations in DNA methylation are common in a variety of tumors as well as in normal development. Of all the epigenetic mechanisms, hypermethylation, which represses transcription of tumor suppressor genes leading to gene silencing, has been the most extensively studied [4–6].
The aim of this study was to explore the association of hypermethylation of ppENK with pancreatic carcinoma and to examine the effects of a demethylating agent on pancreatic cell lines.
2. Materials and Methods
2.1. Cell Lines and Tissues
The human pancreatic carcinoma cell lines Panc-1, Pupan-1, Aspc-1, PC3, and SW1990 were obtained from the Peking Union Medical College Hospital. Thirty-two pancreata surgically resected at the Peking Union Medical College Hospital were selected. Normal pancreatic tissues were obtained from surgical resections at the Peking Union Medical College Hospital. The institutional review committee for clinical investigation reviewed and approved the collection of tissue samples for genetic analysis. Information on the following was available for all the selected cases: age, sex, diagnosis, CA19-9 levels, liver function (especially jaundice), tumor size, location, and clinical stage. Normal pancreatic tissue was taken from autopsy specimens. We examined the association of the methylation status of ppENK with tumor stage, size, location, sex, age, smoking, drinking, and so forth.
2.2. Microdissection of Tumor Cells and DNA Isolation
Two 20 μm serial sections of formalin-fixed, paraffin-embedded tissue from 9 patients were deparaffinized with xylene for 30 minutes (the xylene was changed every 10 minutes) and dehydrated in an ethanol series. The slides were then stained with eosin to facilitate microdissection. Pancreatic ductal adenocarcinoma was identified under a microscope by a single pathologist, and the areas of interest were manually microdissected. An estimated 10% to 20% of the cells collected were surrounding nonductal cells. Nontumor tissue was collected in a separate tube. The microdissected tissues were transferred to a 1.5 mL microcentrifuge tube containing 50 μL of 1x TK buffer (0.05 mmol/L Tris-HCl, pH 8.9; 2 mmol/L EDTA; 1 mmol/L NaCl; 0.5% Tween 20; and 0.2 mg/mL proteinase K) and incubated at 56°C overnight. The tubes were placed in a 100°C block for 10 minutes to inactivate proteinase K. Genomic DNA was extracted from 5 to 10 mg paraffin-embedded tissue using a Puregene DNA isolation kit following the protocol for DNA isolation provided. DNA was quantified by ultraviolet spectrophotometry and stored at 4°C.
2.3. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR)
RNA from the pancreatic cancer cell lines was isolated with Trizol Reagent (Life Technologies, Rockville, MD, USA). Samples of each RNA were reverse-transcribed using a Superscript II kit (Life Technologies). PCR primers were 5′-CCG AAT GCA GCC AGG ATT G-3′ (sense) and 5′-GTG CTG GTG CCA TCT TGA G-3′ (antisense). A 179 bp PCR product was then amplified along with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the following conditions: 95°C for 5 minutes; 35 cycles of amplification (95°C for 30 seconds, 60°C for 30 seconds, and 72°C for 300 seconds); 10 minutes at 72°C. The PCR reaction products were resolved by electrophoresis on 2% agarose gels and stained with ethidium bromide.
2.4. Methylation-Specific PCR (MSP)
ppENK methylation status was detected by MSP. Genomic DNA was isolated from the cell lines and frozen normal pancreatic samples using a tissue DNA isolation kit. Pancreatic carcinoma cells were microdissected from six paraffin-embedded blocks before DNA isolation. DNA was modified by sodium bisulfite as previously described [7].
The bisulfite treatment was performed on each dissected sample (50 μL containing 500 to 10,000 cells) by incubating the DNA at 50°C for 16 hours, as previously described [8]. The modified DNA was purified using a Wizard DNA Clean-up System (Promega, Madison, WI, USA). After that, the polymerase chain reaction (PCR) was performed without ethanol precipitation so as to decrease the loss of DNA [9].
MSP was performed on samples of bisulfite-modified DNA using primers specific for unmethylated ppENK (sense: 5′-TTGTGTGGGGAGTTATTGAGT-3′; antisense: 5′-CACCTTCACAAAAAAAATCAATC-3′) or methylated ppENK (5′-TGTGGGGAGTTATCGAGC-3′ and 5′-GCCTTCGCGAAAAAAATCG-3′). The PCR product was then amplified along with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the following conditions: 94°C for 13 minutes; 45 cycles of amplification (94°C for 30 seconds, 62°C for 30 seconds, and 72°C for 30 seconds); 3 minutes at 72°C. The PCR reaction products were resolved by electrophoresis on 2% agarose gels and stained with ethidium bromide.
2.5. 5-aza-dC
Cells of two pancreatic carcinoma cell lines (Panc-1, Aspc-1) were treated with a final concentration of 1.0 μM of the demethylating agent 5-aza-dC (Sigma Chemical Co.); the Aspc-1 was treated for 96 h and the Panc-1 cells for 120 h. Cell growth was measured by the MTT method, and apoptosis and the phases of the cell cycle were analyzed by flow cytometry after PI staining.
2.6. Statistical Analysis
Each experiment was repeated at least three times, and the experimental data are reported as means ± standard deviations. Statistical analysis was performed with the SPSS11.0 statistical software package. The chi-square analysis, -test, Fisher's exact test, and calculated OR values were used; indicated significant difference. Fisher's exact test was used to analyze the association of the methylation status of ppENK with tumor stage, size, location, sex, age, smoking, drinking, and so forth.
3. Results
3.1. Pancreatic Cancer Tissue
ppENK was methylated in twenty-eight (90.3%) of the 31 samples of pancreatic cancer tissues, whereas it was not methylated in normal pancreatic tissue. There was no correlation between the extent of methylation of ppENK and clinicopathological features of the tumors.
The size of the ppENK methylation target band (M band) is 96 bp (Figure 1).
3.2. The Relation between Extent of ppENK Methylation and Clinicopathological Features of the Pancreatic Carcinomas
There was no correlation between the extent of methylation of ppENK and clinicopathological features of the pancreatic carcinomas such as tumor stage, size, location, sex, age, smoking, and drinking, (Table 1).
3.3. Pancreatic Carcinoma Cell Lines
In the five pancreatic carcinoma cell lines, methylated ppENK was demonstrated and no ppENK mRNA was detected. Methylated ppENK is associated with absence of ppENK mRNA expression.
3.4. ppENK Gene mRNA Expression in Pancreatic Cancer Cell Lines
We examined expression of ppENK using RT-PCR in the 5 pancreatic cell lines. ppENK mRNA was detected in normal pancreatic tissue but not in the five pancreatic carcinoma cell lines (Panc-1, Pupan-1, Aspc-1, PC3, and SW1990) (Figure 2).
3.5. Analysis of ppENK Gene Methylation in Pancreatic Cancer Cell Lines
DNA was extracted from the five pancreatic cancer cell lines (Panc-1, PC3, Pu-Pan-1, SW1990, and Aspc-1), modified by sulfite and amplified by MS-PCR. The results showed that the target band (M band) could be seen in the five pancreatic cancer cell lines. Thus methylated ppENK was detected in all five pancreatic cancer cell lines. Methylated ppENK is associated with the absence of ppENK mRNA expression in pancreatic carcinoma cell lines and normal pancreatic tissue () (Figure 3).
3.6. The Effect of the Demethylating Agent 5-aza-dC on Pancreatic Cancer Cell Proliferation
Based on the MS-PCR results and the origins of the pancreatic cancer cell lines, we chose the Panc-1 and Aspc-1 to test the effect of 5-aza-dC on proliferation. Samples of 103 cells of the two cell lines (Panc-1 and Aspc-1) were seeded in 96 well plates, cultured 1–5 days, and cell numbers were determined using the MTT assay. The proliferation of Aspc-1 decreased significantly (1.608 ± 0.219) in response to 5-aza-dC (1.0 μM) at 96 h (control: 2.644 ± 0.247) and a similar effect could be seen in Panc-1 at 120 h (Tables 2 and 3; Figures 4 and 5).
3.7. The Effect of the Demethylating Agent 5-aza-dC on Apoptosis and the Cell Cycle of the Pancreatic Cancer Cell Lines Panc-1 and Aspc-1
Flow analysis was used to assess the cell cycle of cells. For flow analysis, Panc-1 and Aspc-1 were stained with propidium iodide (PI, Sigma) as described and were used to estimate proportions of cells in the G1/S and G2/M phases of the cell cycle. The frequencies of apoptosis of of Panc-1 and Aspc-1 induced by 5-aza-dC (1.0 μM) were 31.57 ± 6.76% and 16.6 ± 8.22%. In the absence of 5-aza-dC the frequencies were 3.21 ± 1.43%, 3.82 ± 1.71%. The frequency of cells in G1 phase was increased concomitant with a decrease in the percentage of S phase cells in Panc-1 () but not in Aspc-1.
3.8. The Percentage of Apoptosis Detected by Flow Cytometry (PI Staining)
After treatment with 1 μM 5-aza-dC for 5 days, Panc-1 cells were stained with PI and apoptosis was detected by flow cytometry. The average apoptosis percentage was 31.57 ± 6.76% in the treatment group, higher than the control group (3.21 ± 1.43%, ). The average apoptosis percentage of Aspc-1 cells was 31.57 ± 6.76% in the treatment group and 3.82 ± 1.71% in the control group; the percentage apoptosis increased after treatment, but the effect in this case was not statistically significant () (Figures 6 and 7; Table 4).
3.9. Cell Cycle Distribution Detected by Flow Cytometry
Compared with the control group, the proportion of G1 phase Panc-1 cells was significantly increased in the treatment group, and the proportion in the DNA synthesis phase (S phase) was significantly reduced (), but in the Aspc-1 cells there was no noticeable increase in G1 phase or decrease in S phase (Tables 5 and 6; Figure 8).
3.10. Effect of the Demethylating Agent 5-aza-dC on ppENK Gene Expression
After treatment of the 2 pancreatic carcinoma cell lines with 5-aza-dC, ppENK mRNA expression was reversed. The 180 bp target band was detected in the treatment group and the normal pancreas but not in the control group, while the internal reference using GAPDH could be seen (Figure 9).
Compared with the control group using FluorChem (data analysis software), ppENK gene expression in Panc-1 and AspC-1 cells was significantly increased in the treatment group (). The results show that 5-aza-dC can reverse the inhibition of ppENK gene expression.
3.11. Effects of the Demethylating Agent 5-aza-dC on Panc-1 and Aspc-1 Cell Methylation
After treatment with the demethylating agent 5-aza-dC, extraction of cell DNA and modification by sulfite, MS-PCR, failed to detect the ppENK methylation target band (Figure 10).
4. Discussion
The ppENK gene encodes met-enkephalin, which is a tonically active inhibitory factor that interacts with the opioid growth factor receptor. Zagon and McLaughlin reported that met-enkephalin inhibited the growth of several human tumors including pancreatic cancers [3]. Comb et al. reported that methylation of CpG islands within the ppENK gene inhibited its expression by directly interfering with the binding of a positively acting transcription factor [2]. Therefore, methylation of ppENK during pancreatic carcinogenesis may promote cell growth. Here, our aim was to explore the association between hypermethylation of ppENK and pancreatic carcinoma and to study the effects of a demethylating agent on pancreatic cell lines.
ppENK methylation was confirmed in 90.3% (28 out of 31) of the examined samples of pancreatic cancer tissue, but there was no association between methylation and clinicopathological features. Additionally, methylated ppENK was detected in five pancreatic carcinoma cell lines (SW1990, Panc-1, PC3, Aspc-1, and Pupan-1) that showed no expression of ppENK mRNA, which corroborates the findings of previous studies. Overall, these results confirm that silencing of the ppENK gene due to methylation may be an important event in carcinogenesis and the development of pancreatic cancer. However, animal studies and clinical research are warranted to validate this view.
There are several methods of detecting methylation, and each has its own advantages and a range of applications. Currently the most widely used technique is sodium bisulfite modification of DNA and subsequent methylation-specific PCR 10 (MSP), also used in our study. Using two pairs of highly specific primers, this method is able to amplify specific target sequences and distinguish a methylated template from a nonmethylated background with high sensitivity. In fact, as the most sensitive method of detecting methylation, MSP can be effectively used to analyze paraffin specimens, as well as to trace samples such as blood or stool. Therefore, MSP appears to be a useful method for early diagnosis of pancreatic cancer [8, 10–12]. It should be noted, however, that although tumor cell lines are free of the contamination with normal cells otherwise associated with in vivo samples, and they preserve the molecular abnormalities of the tumors and the malignant phenotype, their biological characteristics may change after long-term cultivation. Hence, in vitro and in vivo results should be interpreted together.
Two of the five pancreatic cancer cell lines, Panc-1 and PC3, were derived from primary tumors in earlier clinical stages, while Aspc-1, SW1990, and Pupan-1 were derived from metastatic tumors in later clinical stages. We therefore selected Aspc-1 and Panc-1 for the purposes of this study. Cell proliferation was found to decrease with increasing dose of 5-aza-dC (0.4–1.0 μM), confirming that 5-aza-dC inhibits the growth of pancreatic cancer cells. There was no significant difference between the responses of the two cell lines at a concentration of 1.0 μM 5-aza-dC ().
Apoptosis is an important aspect of the balance between cell division and cell loss. It plays a role in many diseases such as cancer and autoimmune disorders, among others. The results of PI staining and flow cytometry of the Panc-1 and Aspc-1 cell lines in our study showed that apoptosis was triggered by different levels of 5-aza-dC in these two types of cells. Treatment with the demethylating agent resulted in a significantly higher rate of apoptosis in Panc-1 cells than in untreated controls (). In Aspc-1 cells, apoptosis increased from 3.82 ± 1.71% to 16.6 ± 8.22% after treatment, but this effect was not statistically significant (), possibly due to the small sample size.
The malignant phenotype of tumor cells depends, to a substantial extent, on the rate of cell proliferation, which in turn depends on the duration of the cell cycle and on the ratio of cell proliferation to cell death. Thus, in addition to measuring the rate of apoptosis, we should also be interested in any change to the phases of the cell cycle and the proportion of tumor cells that are involved. In our study, Panc-1 cells treated with 5-aza-dC had a longer G1 phase and a correspondingly shorter S phase than untreated controls (). While the same trend was observed in the Aspc-1 cell line, it did not reach statistical significance. It is, therefore, possible that 5-aza-dC could have a different impact on different types of tumor cells, or it could affect different phases of the division cycle. This hypothesis requires further study [13].
In line with previous studies, our findings showed that 5-aza-dC treatment reversed the expression of ppENK mRNA in the pancreatic cancer cell lines Panc-1 and Aspc-1, though to varying degrees (). ppENK methylation could be seen before the treatment, but after treatment it was below detection levels. However, the nonmethylated primers failed to amplify the target band (U band), suggesting that the amount of 5-aza-dC used in the experiment may not have been enough to achieve complete demethylation. MSP assay primers are typically designed based on all CpG sites within a CpG island, but, in fact, not all CpG sites are fully methylated or unmethylated. Because of this inconsistency, it may be difficult to amplify target fragments, and increasing the levels of 5-aza-dC may yield clearer results. Overall, our study provides more evidence that the ppENK gene is inactivated due to methylation and that changes in methylation status may promote cell apoptosis and inhibit the proliferation of pancreatic cancer cells [14–16].
In conclusion, we show that ppENK inactivation due to changes in methylation plays an important role in pancreatic carcinogenesis. ppENK methylation is an important molecular event that could help to distinguish pancreatic carcinoma from normal tissue. Methylation of ppENK may also be affected by cell growth, cell apoptosis, and the duration of the cell cycle.
- K. Brune, S. M. Hong, A. Li et al., "Genetic and epigenetic alterations of familial pancreatic cancers," Cancer Epidemiology Biomarkers and Prevention, vol. 17, no. 12, pp. 3536–3542, 2008. View at Publisher · View at Google Scholar · View at Scopus
- M. Comb, P. H. Seeburg, and J. Adelman, "Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA," Nature, vol. 295, no. 5851, pp. 663–666, 1982. View at Google Scholar · View at Scopus
- I. S. Zagon and P. J. McLaughlin, "Opioid growth factor (OGF) inhibits anchorage-independent growth in human cancer cells," International Journal of Oncology, vol. 24, no. 6, pp. 1443–1448, 2004. View at Google Scholar · View at Scopus
- A. B. Lowenfels and P. Maisonneuve, "Epidemiology and prevention of pancreatic cancer," Japanese Journal of Clinical Oncology, vol. 34, no. 5, pp. 238–244, 2004. View at Publisher · View at Google Scholar · View at Scopus
- A. P. Wolffe and M. A. Matzke, "Epigenetics: regulation through repression," Science, vol. 286, no. 5439, pp. 481–486, 1999. View at Publisher · View at Google Scholar · View at Scopus
- G. Egger, G. Liang, A. Aparicio, and P. A. Jones, "Epigenetics in human disease and prospects for epigenetic therapy," Nature, vol. 429, no. 6990, pp. 457–463, 2004. View at Publisher · View at Google Scholar · View at Scopus
- J. G. Herman, J. R. Graff, S. Myöhänen, B. D. Nelkin, and S. B. Baylin, "Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands," Proceedings of the National Academy of Sciences of the United States of America, vol. 93, no. 18, pp. 9821–9826, 1996. View at Publisher · View at Google Scholar · View at Scopus
- I. S. Zagon, J. P. Smith, and P. J. McLaughlin, "Human pancreatic cancer cell proliferation in tissue culture is tonically inhibited by opioid growth factor," International Journal of Oncology, vol. 14, no. 3, pp. 577–584, 1999. View at Google Scholar · View at Scopus
- T. Ueki, M. Toyota, T. Sohn et al., "Hypermethylation of multiple genes in pancreatic adenocarcinoma," Cancer Research, vol. 60, no. 7, pp. 1835–1839, 2000. View at Google Scholar · View at Scopus
- S. Derks, M. H. F. M. Lentjes, D. M. E. I. Hellebrekers, A. P. De Bruïne, J. G. Herman, and M. Van Engeland, "Methylation-specific PCR unraveled," Cellular Oncology, vol. 26, no. 5-6, pp. 291–299, 2004. View at Google Scholar · View at Scopus
- I. S. Zagon, S. D. Hytrek, C. M. Lang et al., "Opioid growth factor ([Met5]enkephalin) prevents the incidence and retards the growth of human colon cancer," American Journal of Physiology, vol. 271, no. 3, pp. R780–R786, 1996. View at Google Scholar · View at Scopus
- I. S. Zagon, S. D. Hytrek, J. P. Smith, and P. J. McLaughlin, "Opioid growth factor (OGF) inhibits human pancreatic cancer transplanted into nude mice," Cancer Letters, vol. 112, no. 2, pp. 167–175, 1997. View at Publisher · View at Google Scholar · View at Scopus
- R. L. Momparler, "Epigenetic therapy of cancer with 5-aza-2′-deoxycytidine (decitabine)," Seminars in Oncology, vol. 32, no. 5, pp. 443–451, 2005. View at Publisher · View at Google Scholar · View at Scopus
- L. Jiao, J. Zhu, M. M. Hassan, D. B. Evans, J. L. Abbruzzese, and D. Li, "K-ras mutation and p16 and preproenkephalin promoter hypermethylation in plasma DNA of pancreatic cancer patients: in relation to cigarette smoking," Pancreas, vol. 34, no. 1, pp. 55–62, 2007. View at Publisher · View at Google Scholar · View at Scopus
- K. Ohtsubo, H. Watanabe, F. Yao et al., "Preproenkephalin hypermethylation in the pure pancreatic juice compared with p53 mutation in the diagnosis of pancreatic carcinoma," Journal of Gastroenterology, vol. 41, no. 8, pp. 791–797, 2006. View at Publisher · View at Google Scholar · View at Scopus
- H. Matsubayashi, M. Canto, N. Sato et al., "DNA methylation alterations in the pancreatic juice of patients with suspected pancreatic disease," Cancer Research, vol. 66, no. 2, pp. 1208–1217, 2006. View at Publisher · View at Google Scholar · View at Scopus


text:
Oppo R7s Malaysia – Style In A Flash
Dedicated in merging art and science in its products, Oppo has been very successful in designing its smartphones that reflect such statement. Similarly, Oppo R7s is going to transform your mobile experience.
Splendid Display Quality
When Oppo designs Oppo R7s, it utilizes high level of craftsmanship that merges an ultra narrow 2.2mm bezel and 5.5 inch arc edge screen into an amazing smartphone design that is known as Oppo R7s. Such unique craftsmanship makes Oppo R7s to be more stunning than ever as it exudes sophistication vibe. The Oppo R7s is rightly admired for the use of premium metal alloy in its construction. As it turns out, it is not just a simple metal alloy because it has been treated with the state of the art surface coating technology that gives it velvety metallic feel.
As for its 5.5 inch arc edge screen, it certainly completes the overall look of Oppo R7s by making to be more stylish. Not just that, the charming curves of 2.5D arc edge screen add more elegance to its design. Moreover, it transforms Oppo R7s to be comfortable design that you will love to hold.
Snapping Breathtaking Scenes with Oppo R7s Malaysia
Taking pictures with Oppo R7s has never been this easier as its 13MP front camera proves to be more than capable in snapping high quality photos in no time. Each shot that you take will be highly detailed and stunning at the same time. In other words, it is well-suited for capturing your perfect moments that you cherished. As for its 8MP front camera, it is crafted exclusively for you to snap beautiful selfies that are shareworthy across the realm of social media. Besides, you can trust it to show clearer picture quality especially during video chats.
Getting The Ultimate Selfie Effortlessly
Aside from possessing powerful cameras that specialize in capturing perfect moments, Oppo R7s also comes with tons of tools that you can use in order to enhance and transform your pictures into the way you that desire. For even more stunning selfies, you can use the incredible Beautify 3.0 that transforms your selfies to be true works of art. Your mobile photography has been totally different as you can apply numerous filters, Ultra HD, double exposure and other modes in order to get the desired effect. In short, Oppo R7s makes it possible for you to enjoy snapping photos like a true professional photographer.
Dual SIM For Leading Double Lives
If you are in favor of separating work and play lives, Oppo R7s gives you the good news since it has Dual SIM slots so that you can truly enjoy the meaning of work-life balance. In short, Oppo R7s is truly amazing when you use it to make the perfect between home and travel or work and personal lives. Hence, Oppo R7s is the stylish and stunning smartphone with great qualities.


text:
ACParadise Network: American Cosplay Paradise American Cosplay Experience American Cosplay Snapshots
Cosplayer Eve > Costume of Sirius Black (Harry Potter)
- Most Recent Photo
- 11-16-2011
- Series
- Harry Potter
- Character
- Sirius Black
- Special Variation:
- Marauders
- Year Completed:
- Construction Difficulty:
- Costume worn at:
- Yaoi-con 2011
About this Costume
- Construction Details:
Was given a few fan renditions and decided on an "clean mess" kind of interpretation. Laid back style, but not a dirty hobo. I liked the idea of long-ish hair given his nickname and having it look a little wake up and brush fingers through. His movie version didn't have him button his top button a lot, so also used that even if Ello (Snape) criticized it, which was very funny and in character.
- Personal Thoughts:
- Happy Potter and the Chamber of Giggles- wait, no one invited you, Peter.
Add a Comment


text:
Landscapes for Life: A Homeowner's Guide to Sustainable Gardening
Sponsor(s): Sarah P. Duke Gardens
Cost: $180; $145 Gardens members and Duke students/staff.
Learn how to work with nature in your garden, harnessing its power to create a healthy and beautiful home landscape. Landscape architect Jesse Turner will guide you through each step: getting started, assessing your garden soil, hydrology, plant choices, and garden materials. Each stage includes worksheets and complete assessment information. The program materials were developed by the U.S. Botanic Garden and the Lady Bird Johnson Wildflower Center and modeled after the Sustainable Sites Initiative benchmarks. All materials included in the fee. The 5-part class continues on Wednesdays through Feb. 13. Pre-registration required. Parking included. (919) 668-1707.
Type: SUSTAINABILITY, WORKSHOP/SHORT COURSE, and ALUMNI/REUNION
Contact: Sara Smith


text:
Accepting Guests
- Last login 3 months ago
Join Couchsurfing to see Ilia's full profile. It's free!
About Me
don't hope for a better world, make it.
create your own visited country map
or check our Venice travel guide
i am a traveler like we all are i think, even when i dont go somwhere i am on the way to some thing, or my thoughts wonder to e distant corner of the planet. i study anthropology, and african studies, and do many other things like taking photos, writing for some newspaperes, climbing mountains playing chass end ocasionaly interested in almost eny thing, but mostly intrigued about every new person i meet, so meet me and form your own opinion :)
don't watch the news. make them.
Why I'm on Couchsurfing
well by hosting people of course ... but also showing around the city or mitting for a bear :)
well i have housted till now some 5 or five people in the last mounthes...
traveling (well that's a bit obvious isn't it) photography, activism, voluntarily, reading, quality muevees, hanging out, writing, collecting things, climbing.
- writing
- photography
- news
- reading
- traveling
- origami
- rock climbing
- anthropology
- tourism
- volunteering
- mountains
Music, Movies, and Books
music : reggae, folk punk, gypsy music, balkanic
books : hundred years of solitude, hitchhikers guide for the galaxy, 1984, on the road, bugy samurai, master and margarita.
movies: y tu mama tambien, children of men, forest gamp.
One Amazing Thing I've Done
well the most amazing so far was volunteering in an HIV orphanage in ethiopia
Teach, Learn, Share
teach : tarot, astrology, russian, hebrew, spanish, english, origami
learning : i want to learn almost everything !!!
Countries I've Visited
Belarus, Belgium, Cyprus, Denmark, Estonia, Ethiopia, Germany, Greece, Iceland, Latvia, Luxembourg, Mexico, Moldova, Netherlands, Palestine, Romania, Ukraine
Countries I've Lived In
Germany, Israel, Russian Federation


text: Choi is a successful doctor who remains afflicted by the pain he feels over his parents' deaths when he was a child. Kim is a friendly girl who shares Choi's turmoil as an orphan, and has a terminal case of stomach cancer. When these two meet in a bar one night, they make a connection that fills a hole they've both been carrying all their lives, but their time together will be decidedly finite. Now these two kindred spirits must decide how to spend the short but incalculably important time they have together.


text:
Results 11 to 15 of 29
July 6th, 2013 01:43 AM #11Senior Member
- Join Date
- Mar 2013
- Posts
- 849
Hmm, well in general i judge children by how they behave, as another poster said up-thread. But i do sort of assume things about the parents sometimes. Like I've yet to meet a Paisley (and all alternate spellings) whose mom wasn't an airhead whose biggest concern in life is preparing her 2-year old to be a future cheerleader. That isn't to say that every person who chooses the name Paisley is like that, but I've met enough that i sort of assume it with some names.
It wouldn't ever affect how i treat a child or their parents, and I'm always happy to be proven wrong. But sometimes i make those assumptions, and i don't think there's any harm in that if it isn't coming from a mean place. All the moms of Paisleys i know are lovely people and fun to talk to...just have different aspirations for themselves and their kids, and that's fine. Like i said, as long as it doesn't affect how you treat someone, i think having that assumption isn't in itself a bad thingI hope to be a mom one day. For now I enjoy being a name lover.
My apologies for any typos; i post from my mobile phone.
July 6th, 2013 06:15 AM #13
No. You are not your name. Your name could be changed this very moment and you would be the exact same person you are. IMO, Tyler and Brendan are perfectly fine names and any guy berries with those names, right on. She would probably tell me that my name (Alexis) is horribly trashy. This lady needs to calm down. I hope little Maximilian marries a Kylie, and Poppy and India have boyfriends named Tyler and Brendan.Alexis
teen berry
excuse my username
Isla Juno - Elodie Clover - Genevieve Aisling- Juliet Sparrow
Wolfgang Pascal - Evander Hugo - Jasper Fox - Theodore Blaise - Lucian Atlas
July 6th, 2013 06:21 AM #15
Yes. Yes I do. But then I have a conversation with them and reassess the situation.I'm not feeling incredibly profound at the moment. Check back later.
July 6th, 2013 06:27 AM #17Senior Member
- Join Date
- Oct 2012
- Location
- Australia
- Posts
- 256
No judgment to the child....but another story for their parents!Mummy to the gorgeous Alice Heather and the delicious Harry George
Bubba #3 due March 2014! Finalising short list of favourite names!
July 6th, 2013 06:45 AM #19
Katie Hopkins is the most irritating, outrageous woman I've ever seen on national television. Not only did she make a nation full of people hate her, she's made herself look like a complete idiot by saying she hates geographical location names and named her daughter India. She is also judging children by their looks by saying you can't name a child without looking at them and it's stupid to name a child Bella if she's ugly.
This woman is a serial attention seeker and will do anything for publicity, I suggest to anyone to research her background, she's not the higher class 'lady' she thinks she is.
I work in a school filled with children named Tyler, Jayden, Angel and Brandon not all of them come from lower class families. Some of these children have a lot of potential for the future and are bright, beautiful and young individuals who shouldn't be judged just because of their names.
I guess you can change your name but not your ugly personality.Libby Felicity Annalise Isla Phoebe Carina Violet Martha Ariana | Iowan Macsen Henry Isaac Jude Freddie Aneurin Niall Adair


text:
|Ludroth Topics:
Ludroth are Leviathans introduced Monster Hunter 3.
Ludroth are mostly yellow-green in coloration, with long sinewy bodies that enable swift and sweeping underwater movements to confuse their prey. Their claws are stubby but effective, and their strong tails end in reinforced bony segments.
Powerful jaws allow for moderately strong biting attacks.
The majority of Ludroth are female, and form harems around the male Royal Ludroth.
Ludroth are capable of producing globules of mucus that they can spit at enemies. The mucus inflicts Waterblight on Hunters, which slows the speed of stamina recovery.
Ludroth are amphibious, and should not be underestimated on land. They are capable of using their almost serpentine forms to slither and pounce on Hunters. They emit a barking noise when threatened, and although dangerous on land, are doubly so when confronted underwater. Notably more powerful, swifter, and with attacks that may disorient a hunter caught unaware, a Ludroth in her natural habitat can be a challenge; small crowds of Ludroth are also more often found underwater, and can quickly part even an experienced hunter from a good volume of their health.
Ludroth are found in the Flooded Forest and Deserted Island because there is an abundance of food and an agreeable mixture of coastline and aquatic environment for them to use as territory.
Game Appearances
In-Game Description
- Ludroth are also afraid of Torches. While holding a Torch, they won't attack and keep moving backwards. The same effect is induced when Cha-Cha wears the Lamp Mask. Also, if the Ludroth is struck twice with a Torch, it will flee by digging down in the ground.
- If hit by Dung Bomb, Ludroth will again tunnel into the ground.
- Ludroth can be summoned (in only a few Areas) when Royal Ludroth appears.
- Ludroth can be stunned.
- If a Ludroth has targeted a hunter in area 11 of the Deserted Island, and the hunter climbs onto the vines and is above the water surface, Ludroth will swim around a bit, and then lunge out of the water, knocking the hunter off the vines. Ludroth will land back in the water.


text:
First book sale will benefit library
After several years of hard work, the Friends of the Robert Trail Library finally will get to help the library. The group will hold a book sale April 14-16 and the money raised through the event will go towards library needs.
It's been no small task getting the organization up and running but for FORT president Jo Gilbertson the toil has been worth it.
"To see it finally come to fruition is so great," said Gilbertson.
The Friends of the Robert Trail Library received its 501(C)3 designation last year. The volunteer group is dedicated to promoting literacy and lifelong learning through enhancing library services and resources. Gilbertson said the group hopes to do that by providing financial help for things like author visits or equipment costs.
Another cost for the group is to get a web site up and running. Gilbertson said to get a domain name that's easy and effective the group will have to pay.
"We'd love to have help with a web site," said Gilbertson.
The book sale will be the group's first fundraising effort. That too has been a lot of work. Gilbertson said the community has been generous with their donations and sorting and moving all the books for the sale has been a lot of work.
"It's been a laborious process, but I am looking forward to having a great crowd," said Gilbertson.
Leading up to the book sale, FORT collected as many books, CDs, VHS tapes and DVDs as possible. There were drop off boxes at area businesses. In addition, Boy Scouts went through some local neighborhoods collecting.
"We are grateful for the donations," said Gilbertson.
The book sale will be held April 14-16. A preview will be held from 4 to 8 p.m. April 14. Admission will be limited to members of FORT, or with a paid admittance or membership purchase of $5.
Gilbertson said they would be glad to add members to their group through the effort.
The official sale will begin at 10 a.m. April 15 and will run until 5 p.m. both days. Hardcover books will sell for $1 each. Paperbacks will be priced at 50 cents. CDs and DVDs will be $2. VHS will be $1 each.
There will be a bag sale from 3 to 5 p.m. April 16. Shoppers can buy a bag and fill it.
The group is taking on new members. First-year membership is $5. Membership forms can be found at the Robert Trail Library. FORT meets at 6:30 p.m. the second Monday of the month.
For more information email President Jo Gilbertson at firstname.lastname@example.com.


text:
Sheryl Crow, Tim McGraw to play in Sioux Falls
SIOUX FALLS -- It's not going to be a quiet weekend in Sioux Falls.
JazzFest 2013 will feature everything from local acts to superstar Sheryl Crow at Yankton Trails Park. In a separate event, country music star Tim McGraw will perform at the W.H. Lyon Fairgrounds.
According to a press release from the Sioux Falls Jazz and Blues Society, this summer is the 22nd annual JazzFest. The festival kicked off Thursday and will continue today and Saturday with a full schedule of activities and musical performances.
The three-day event will culminate at 10 p.m. Saturday with a Sheryl Crow concert, and fireworks at 11:30 p.m.
According to the event's website, JazzFest is a "free and fun event for the whole family." In addition to music throughout the weekend, the event features a 5K Run/Walk, One-Mile Fun Run/Walk and the Sanford Children's JazzQuest, which provides children opportunities to learn about music, play and create hands-on art projects. The event is a partnership between JazzFest and the Washington Pavilion of Arts & Science.
Also on Saturday, country music star Tim McGraw will perform at 7:30 p.m. at the W.H. Lyon Fairgrounds in Sioux Falls. The concert is sold out, according to the artist's website.
JazzFest schedule:
--Today, main stage
6 p.m. -- Scott Holt Band
8 p.m. -- Chubby Carrier and the Bayou Swamp Band
10 p.m. -- North Mississippi Allstars
--Today, second stage
6 p.m. -- Chris Borchardt
7:30 p.m. -- Mind Machine
9 p.m. -- Blues Bashers
--Saturday, main stage
12 p.m. -- The Hegg Brothers Band
1:30 p.m. -- JazzFest Jazz Camp with Chris Vadala
3 p.m. -- Kris Lager Band
4:30 p.m. -- Mingo Fishtrap
6 p.m. -- BeauSoleil avec Michael Doucet
8 p.m. -- Doyle Bramhall II
10 p.m. Midcontinent Communications Presents: Sheryl Crow
--Saturday, second stage
Noon -- JazzFest Jazz Camp
1:15 p.m. -- The Coopers
2:45 p.m. -- FTX
4:15 p.m. -- Kyle Knutson
5:45 p.m. -- White Dads
7:15 p.m. -- Hegg Brothers
9 p.m. -- Phantom Balance
Sanford Children's JazzQuest schedule:
6 to 8:30 p.m. -- Inflatables open
Noon -- Inflatables open
2 p.m. -- Discovery Mime Theater
3 p.m. -- The Coopers Instrument Petting Zoo
4 p.m. -- Discovery Mime Theater
5:30 p.m. -- "On Baker Street!" Live performance with Phil Baker
9 p.m. -- Inflatables Close


text:
The Nike Air Penny II may have just dropped, but UNDRCRWN wanted to take a moment to honor a different 90's icon. "Long before there were ever Kobe & Lebron muppets – there was one very famous NBA party crasher. To honor this very memorable sneaker release, UNDRCRWN pays homage to basketball's most iconic marionette alongside one of Brooklyn's finest. If you didn't already know this, look up the lyrics to "Can I Live" and the back of the shirt will make sense!" The t-shirt comes in black, white, grey heather and royal blue. Visit UNDRCRWN.com for more information on availability.
A closer look at the UNDRCRWN "M.V.Penny" T-Shirt can been seen after the hop and skip.


text:
You've probably never seen a dating show like this before!
This week, VH1 is launching the new reality program Dating Naked; a show that documents strangers as they go on tropical blind dates.
Oh…did we mention their also completely naked the entire time?!
Here's the show's description courtesy of VH1.com:
"We will follow along as two primitive daters each go on a total of three naked dates, including their first date with each other. At the end of this experience they'll choose which of their naked dates they would like to continue dating back home. Clothing will of course be optional."
Naked Dating premieres this Thursday (July 17)! Will you watch?! Be honest!


text:
|Close Window
Monsanto trapping coyotes to help control population
Kyle Barnett - Feb 07, 2013
In recent years, coyotes have been blamed for a number of missing pets both on the East Bank and West Bank of St. Charles Parish. Now, Monsanto is trying to help control the coyote population by trapping the predators at the Monsanto facility in Luling.
Warren Fremen, co-chair of the plants wildlife committee, said six of the animals have been caught over the past two months.
"We have 1,100 acres of perfect land for them," he said. "We have no control over them at the plant. They breed at will and they have no natural predators."
Fremen said trapping on the property began as a cooperative agreement between the plant and the St. Charles Parish Sheriffs Office after deputies found a coyote that had been run over on a road adjacent to the plant.
"We had rules and there was absolutely no trapping in the site," Fremen said. "We had to talk to the plant manager to get the program approved."
The plant has since been running a coyote relocation program in concert with the Sheriffs Office to remove the predators from its grounds.
Fremen said because no hunting or trapping has been allowed at the plants nature preserve, a community of coyotes has grown over the past decade.
"The problem is the coyotes have gone unchecked for the past ten years," Fremen said. "Weve estimated there are probably 60 to 100 coyotes. So we are trying to get rid of them."
Jeremiah Davis, who works with the K-9 unit of the Sheriff Office, sets up traps for the animals that include motion sensors.
"You have a camera that detects any motion in the trap," Fremen said. "So basically in the off hours or on the weekends if they catch a coyote an email gets sent to his cell phone. Its all very high tech"
Fremen said he is happy with the results so far and he thinks that the relationship between Monsanto and the Sheriffs Office will continue to produce results with Davis doing the trapping.
"We are very pleased with the job he is doing," Fremen said.
Coyotes have been blamed for the disappearance of a number pets in the parish, most notably a Siamese cat owned by Sheriff Greg Champagne. Angie Robert, the animal control director for the parish, said that construction around the parish is forcing coyotes into neighborhoods.
Robert said coyotes are prevalent year round, but that they may appear more common in the summer because the offspring are getting bigger. She added that coyotes can be out at all times of the night and day.
"I have walked in on a coyote in broad daylight that was 20 feet from me," she said.
Robert said the best thing residents can do to avoid their pets becoming a coyotes next meal is keep them inside.
"If you keep (pets) outside they will become a part of the food chain," she said. "People should keep garbage and any potential food source, like pets, inside or protected. When there are no food sources, coyotes will no longer go there."
Read More Stories at HeraldGuide.com!
|heraldguide.com is a supplement to St. Charles Herald Guide.
Copyright © 2001 - 2017 St. Charles Herald Guide, Inc. All rights reserved.
Please contact our WebMaster if you experience problems with the website.