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"It is most definitely expensive, it definitely causes a problem and would be great if it were addressed in some way," said Richard Stiennon, vice president of threat research at anti-spam, anti-spyware vendor Webroot Software.
Lost productivity; additional hardware; additional software licenses (including anti-spam and Exchange servers) to handle the massive amounts of unsolicited bulk emails that inundate network email gateways; the cost of IT staff needed to provision, maintain and monitor all that extra hardware and software; helpdesk calls from employees who really thought they were "Winners" ... it all adds up to a substantial number.
Based on these factors, said Richi Jennings, an analyst with Ferris, "[o]ne can reasonably argue that our cost estimates err on the conservative side."
If you add in the cost to network carriers, Ferris' numbers may indeed be even higher.
According to Stiennon, worms, viruses, spam and other unwanted Internet traffic like phishing attempts and Trojans account for up to 50% of carriers' network traffic. And this is forcing many to lay new lines, which, in turn, costs money, which, in turn, raises customers' prices.
Then there is the cost of regulatory compliance that requires companies to archive email. The more useless emails that are not blocked by anti-spam technology, the more time-consuming and expensive it is to store them, said Chris Miller, director of product marketing for anti-virus vendor Symantec.
"I have to archive all communications and any mail I accept, technically, has to be archived," he said, referring to financial regulations such as Sarbanes-Oxley.
Finally, there are the costs associated with the lost productivity of your IT staff, said Ted Anglace, senior security analyst with anti-virus/anti-spam vendor Sophos. What other projects could they be working on instead of baby-sitting extra Exchange servers (currently the most popular email server for business)? Would you even need as many staff if there were less spam?
This includes helpdesk calls and clean-up costs from employees who may, however unwittingly, by-pass all of your expensive and thorough precautions by bringing to work some portable storage device and launching a spam-based worm/virus/trojan/spyware into the network.
"Another way to look at it would be to say, 'Well, what things are not getting done that companies would like to get done?'" said Anglace. "It's this whole ecosystem where you tug on one little node of it and it ripples through the whole (company)."


text:
Jermain Taylor arrested in shooting investigation ahead of world title fight
Last Updated: 27/08/14 8:22am
Former world champion Jermain Taylor has been arrested following a shooting at his home in Arkansas.
The 36-year-old American, nicknamed 'Bad Intentions', remains in custody on charges of aggravated assault and first-degree domestic battery after shooting his cousin and shooting at the cousin's friend.
Reports have suggested that Taylor's cousin is in a serious condition in hospital, but the other man was not injured.
Lt Carl Minden, a spokesman for the Pulaski County Sheriff's Department, said that an altercation broke out after the two men paid a visit to Taylor's home in Little Rock on Tuesday afternoon.
"After talking to Mr Taylor, who was very cooperative with the investigation, based on the circumstances, it was decided to charge him," Lt Minden told USA TODAY Sports.
"His lawyers were present. The prosecutor was consulted."
The former undisputed middleweight champion had been due to challenge Sam Soliman for the IBF title on October 8.
Taylor will appear before a judge on Wednesday.

subset: commoncrawl


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The Coalition / No Mutiny - for Now
After a tiring day of running around, and of courting and lobbying people, Prime Minister Ehud Olmert's associates were able to report to the boss last night, cautiously, that things are under control on the political battlefield. Unless the Winograd report on the Second Lebanon War, which is being released today, packs a surprising punch that changes everything, no one in Kadima will raise the banner of mutiny and demand Olmert's dismissal.
Foreign Minister Tzipi Livni is worried about getting sullied; Shaul Mofaz, the former defense minister and current transportation minister, is carrying around the weight of six years of neglecting the army; Public Security Minister Avi Dichter is loyal; and even if Housing and Construction Minister Meir Sheetrit says something - so what? With rivals like these, who needs friends?
But Olmert knows that they are just biding their time - not because of the failure of the war, but because they see him as a burden, an albatross, a political weight that is dragging them down to the depths.
The prime minister is liable to survive the draft report not just because of the confusion and helplessness pervading Kadima, but also because of the alternative: Benjamin Netanyahu. The Likud chairman is Olmert's life-saving drug.
No one in Kadima - or the coalition at large, at this stage - wants to shake up the system and bring about elections in which Netanyahu would be voted in as prime minister. Even Ehud Barak and Ami Ayalon, the leading candidates in the Labor primaries, won't take such a responsibility upon themselves. As long as Olmert survives, they will be with him - and if not him, then with Livni or Shimon Peres. Paradoxically, Barak and Ayalon prefer Olmert; it's better to be a strong defense minister serving under a weak prime minister.
If the Kadima revolt has indeed ended before it even began, that leaves the public at the center of attention. Will "the street" force the politicians to do something? There are such high expectations of a wave of public demonstrations that anything less than a mass protest of the kind held in the summer of 1982 will be deemed apathetic. The rally planned for Thursday at Rabin Square in Tel Aviv is meant to light the torch that will burn Olmert's seat out from under him. But here, too, there are problems.
Sources connected to the rally have complained about difficulties in getting people to attend and in raising money. In the meantime, the two most prominent politicians planning to participate in the event are Netanyahu - after all, it's basically his party - and Meretz-Yachad chairman Yossi Beilin. Many of Beilin's colleagues oppose his participation alongside Netanyahu.
Barak is also leaning toward not attending; he doesn't see himself as a sidekick. Today or tomorrow he is due to consult with the ministers who support him. Even if he doesn't show up at the demonstration, he'll make his position known about the report, about those responsible for the war, and most importantly: about what he plans to do if he wins the May 28 primaries.
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The popular author shares her Christmas twist on a classic treat.
- Posted on Nov 8, 2012
This cranberry-based dessert has the look and flavor of Christmas.
|2 ½ c. fresh or frozen cranberries
|12 Tbsp. (1 ½ sticks) unsalted butter, melted
|¾ c. chopped walnuts
|¼ tsp. almond extract
|½ c. plus ¾ c. granulated sugar
|1 c. all-purpose flour
|2 large eggs
|Pinch of salt
1. Preheat oven to 350°F.
2. In a 9-inch pie pan combine cranberries, walnuts and ½ cup of sugar; toss until coated.
3. In a medium bowl, whisk eggs, melted butter, remaining sugar and almond extract until blended. Fold in flour and salt until combined.
4. Pour the batter over the cranberry mixture. Bake for 40 minutes, until crust is golden and fruit bubbles.
5. Transfer to a wire rack to cool.
Serves 8.
Nutritional Information: Calories: 390; Fat: 25g; Cholestrol: 95mg; Sodium: 200mg; Total Carbohydrates: 39g; Dietary Fiber: 3g; Sugars: 24g; Protein: 5g.


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The monarchy of Bhutan is a landlocked state situated in the eastern Himalayas between China and India. Area: 47,000 sq km (18,150 sq mi). Pop. (1996 est.): 842,000 (excluding Nepalese residents declared stateless by the Bhutanese government in late 1990, nearly 100,000 of whom are now refugees in Nepal). Cap.: Thimphu. Monetary unit: ngultrum, at par with the Indian rupee (which is also in use), with (Oct. 11, 1996) a free rate of 35.65 ngultrums to U.S. $1 (56.16 ngultrums = £ 1 sterling). Druk gyalpo (king) in 1996, Jigme Singye Wangchuk.
In April 1996 Bhutan and Nepal held their seventh round of ministerial talks on the repatriation of nearly 100,000 Bhutanese refugees of Nepalese origin who had taken shelter in eight UN-monitored refugee camps in eastern Nepal. As with the previous talks, the participants failed to reach an agreement, particularly on the criteria for determining Bhutanese citizenship. The refugee problem developed after Bhutan launched in 1988 a national policy demanding that everyone adhere completely to Bhutanese Buddhist traditions. Bhutanese of Nepalese origin claimed that this policy was an attempt to suppress Nepalese culture, and, accordingly, thousands fled to Nepal.


text:
PRS S2 Standard 24 Satin Electric Guitar Charcoal Satin
(Charcoal Satin)
Sku #J31861000001000
Solid Body Electric Guitars
- Write a Review
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A new approach to a timeless classic.
The original PRS Standard, introduced in 1987 evolved from Paul Smith's pre-factory all-mahogany guitars. The S2 Standard 24 Satin takes that classic platform and reimagines it for the modern player. While the sleek satin finish and pickguard-mounted electronics will have you looking good, our HFS and Vintage Bass pickups will have you covered sonically. On stage or in the studio, the resonance of the all-mahogany satin body will shine through your amp or pedalboard. Includes gig bag.
Body shape: Double cutaway
Body type: Solid body
Body material: Solid wood
Top wood: Not applicable
Body wood: Mahogany
Body finish: Satin
Orientation: Right handed
Neck shape: Pattern regular
Neck wood: Mahogany
Joint: Set-in
Scale length: 25 in.
Truss rod: Standard
Neck finish: Satin
Material: Rosewood
Radius: 10 in.
Fret size: Medium
Number of frets: 24
Inlays: Dot
Nut width: 1.656 in. (42 mm)
Configuration: HH
Neck: S2 HFS Vintage bass
Middle: Not applicable
Bridge: S2 HFS treble
Brand: PRS
Active or passive pickups: Passive
Series or parallel: Switchable
Piezo: No
Active EQ: No
Special electronics: Push/pull tone control
Control layout: Master volume, tone
Pickup switch: 3-way
Coil tap or split: Coil tap
Kill switch: No
Bridge type: Tremolo/Vibrato
Bridge design: PRS S2 tremolo
Tailpiece: Not applicable
Tuning machines: PRS S2 locking
Color: Nickel
Number of strings: 6-string
Special features: Pickups
Case: Gig bag
Accessories: None
Country of origin: United States
Grab this affordable PRS with all the right stuff. Order today.


text: Dr Emma Robinson In 'Medical and Dental Sciences' College of Medical and Dental SciencesAbout the CollegeUndergraduatePostgraduateMOOCSInternationalOutreach and Widening ParticipationPERCATAlumniContact us Director of Operations BSc (Hons), PhD Dr Emma Robinson qualified with a BSc (Hons) in Biological Sciences from the University of Durham in 1995. She went on to study for a PhD in Molecular Microbiology and to carry out postdoc research at the University of Durham. Subsequent appointments in the Biotech industry and for the Medical Research Council in Oxford confirmed her move out of the laboratory into an academic management role. Dr Robinson was appointed as Research Manager at the University of Birmingham Medical School in 2004, followed by progression to Head of Research & Knowledge Transfer for the College of Medical & Dental Sciences in 2008 before appointment as College Head of Operations in January 2010. Tel: +44 (0)121 414 3179 Email: firstname.lastname@example.com


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may 15
Erik Estrada Yes, CHiPs fans, Ponch is back. He's traded in his Kawasaki and police baton for the suave silken bathrobes of the Mexican telenovela Dos Mujeres, Un Camino. And to celebrate his return to the fringes of celebrity, he's written a tell-all, My Road from Harlem to Hollywood. Tonight, as he signs your copy, you can ask those burning questions about life as a '70s beefcake, fights on the set of CHiPs, his near-death experience and what his former co-hunk Larry Wilcox is doing now. 7 p.m. Borders Books, Music & Cafe, 12788 Fountain Lake Circle, Stafford, (281) 240-6666. Schmoozing is free; the hardback is $22.
Sonny Throckmorton Songwriters Festival Sonny Throckmorton -- the songwriter responsible for the Judds' "Why Not Me?" and a slew of other country hits -- hosts this annual gathering for aspiring hit machines. The seminars and workshops will be led by impressive pals of Throckmorton's, people such as Gretchen Peters, author of Martina McBride's "Independence Day" and "My Baby Loves Me." But with showcase bands such as the Harry Fish String Band and the Hollisters, trainees might find themselves too busy dancing to take notes. Through Sunday. Brazos Bottom Bar & Grill, 7010 FM 762, Richmond, 781-5182. $10 daily; $25, festival pass.
may 16
Greek Festival Need to release some stress? Want to see thin, fragile discs hit hard concrete and shatter into a thousand tiny pieces? Watch local notables indulge these destructive impulses at the St. Basil the Great Greek Orthodox Church Festival's Grand Opening Plate Breaking Ceremony. If they clean their plates of authentic gyros and baklava, they get to smash them with a happy dance and yell "Opa!" Those without Greek heritage are highly encouraged to attend. After all, event coordinators admit, plate-breaking is "an American thing." Festival opens at 10 a.m. with food, dancing, face-painting, moonwalking, games and performances. Plate-breaking at 11 a.m. 915 Eldridge. 866-8883. $2.
World's Largest Movie Theater With 112,000 square feet, 30 wall-to-wall screens, stadium seating with extra legroom and Sony Dynamic Digital Sound, the spanking-new AMC Studio 30 claims the record. You can be among the first to worship at this temple of mass media commercialization: Tonight's "sneak-a-peek" lets you check out not just the theater but 30 flicks ranging from Mission: Impossible to Fargo. Remember, being first is almost as important as being biggest. 6-10 p.m. AMC Studio 30, 2949 Dunvale between Westheimer and Richmond. Call KRBE at 266-1000 for more information on the preview. $.25 -- yes, that's 25 cents.
Student Sale at the Glassell School of Art Through Sunday, the Glassell's professional-level Studio School will offer for sale its artists' best paintings, photographs, sculptures, ceramics and jewelry -- stuff that's much more likely to appreciate in value than those flowery watercolors you'd buy at the Holiday Inn's starving artist blowout. 5-8 p.m today. 10 a.m.-5 p.m. Saturday and 12-5 p.m. Sunday. Glassell School of Art, 5101 Montrose, 639-7500. Free admission.
Houston Met Spring Concert From the ashes of the Delia Stewart Dance Company, Michelle Smith and Dorrell Martin have resurrected the Houston Met. Delia's traditional jazz is mixed with ballet and modern dance, and dancers are picked for their capacity to "emote" rather than for toothpick legs. The Spring Concert features playful, energetic and comedic pieces by nine choreographers who hail from as near as HSPVA and as far as Chicago. 8 p.m. tonight and Saturday. Family Performance 7 p.m. Sunday. Heinen Theater, 3517 Austin at Holman, 522-6375. $5-$15.
String Cheese Incident The Rocky Mountain ski bums of String Cheese Incident are said to make serious use of hula hoops and describe their music as "funkalatino-Afrojazzadelic bluegrass." Think Satellite favorite Leftover Salmon, only less Cajun and more Latin. Best of all, SCI opens for the perennially wacky Banana Blender Surprise. 9 p.m. Fabulous Satellite Lounge, 3616 Washington Ave., 869-COOL. $6.
may 17
Texas Lesbian Conference Rednecks, conservatives and impressionable children, run for your strait-laced lives! Hundreds of lesbians converge on one hotel to celebrate their visibility and empowerment. The weekend-long tenth anniversary conference has everything from inspirational speakers (such as discharged military nurse Colonel Margarethe Cammermeyer) to playshops on hot sex in the '90s ("You'll never look at fruit the same way again!"). Tonight, comedienne Lea DeLaria performs bits from her CD, Bull Dyke in a China Closet, and the Dallas band II Girls, II Guitars, II Much rocks same-sex couples into the wee hours of morning. 7 p.m. Renaissance Houston Hotel, 6 Greenway Plaza East. $15 for tonight's entertainment. $70, conference registration; $55, hard times discount. Call 867-3934 for more information.
Boozoo Chavis The official "King of Zydeco," as elected by the Louisiana Music Hall of Fame, heads an afternoon of down and dirty, hot and sweaty, homegrown musical treats with performances of chanky-chank music by Peggy Scott Adams, Patrick Green, J. Paul Jr., Geno Delafose and Brian Jack. Boozoo, a 67-year-old accordion-wielding Louisiana native, wrote zydeco's first hit single in 1954: Paper in My Shoe. His inspiration? "My feet were cold," he explains. "Didn't have no socks or none of that ...." 2 p.m. Crosby Fairground, FM 2100 Crosby-Lynchburg Rd. in Crosby, (281) 530-9843. $15 pre-event, $18 at the gate, $6 kids under 12.
The Great Monopoly ChallengeThis regulated two-round Parker Brothers tournament is not child's play, so brush up on your money-mongering. (Does Donald Trump have a motivational video? Now's the time to find out.) Top winners can exchange their play money for real trips or jewelry. Arrive early so you can call dibs on the silver car game piece and cruise stylishly past jail and onto Boardwalk. A light dinner will be served before the games begin, and all proceeds go to the Jewish Community Center's special-needs programs for the physically and mentally challenged. 6 p.m. The Houstonian Club, 111 N. Post Oak Lane. Sponsor a table of four to six players for $500 or come alone for $50. Call 729-3200 for information.
The Critical Response Workshop Everybody's a critic, and the tender feelings of sensitive artistes -- choreographers, dancers, actors, directors and playwrights -- are often bruised by indelicate critiques of their works-in-progress. Political activist and dancer Liz Lerman invented a way to keep those egos from being stomped too hard. Her "Critical Response" method, which lets the artist control both the pace and content of a discussion, is designed to create a strong bond between artists and their peers. Houston choreographers Linda Phenix and Sarah Irwin act as guinea pigs for a workshop that's open to the public. If you go, they ask that you leave your "language of judgment" at home. 2-6 p.m. DiverseWorks, 1117 East Freeway, 228-0914. $5 general admission.
Gulf Coast Volleyball Tournament Summer's here, and with it comes the chance to get sand in your bathing suit, paint your nose with zinc oxide and watch men in Speedos grunt and spike volleyballs at each other. If this sounds less than exciting, enter the men's and women's doubles tournament yourself. Check-in is 8-9 a.m; games start at 9:30 a.m. $15 advance registration for players; $25 at the gate. Free to watch. Stewart Beach, Galveston Island, (281) 482-1376.
An Evening of Art Songs and Arias New York-based soprano Camille Zamora, an HSPVA graduate and former Omega House volunteer, has organized a concert to benefit the AIDS hospice. Croatian pianist Dalia Lazar, baritone Randall Scarlata, Lester Lynch, former Houston Grand Opera soloist Thomas Laine, soprano Sharyn Pirtle, German soprano Stefanie Krahnenfeld and Zamora herself will perform songs from works including Don Giovanni, The Barber of Seville and Candide. 8 p.m. The Menil Collection, 1515 Sul Ross, 523-7110. $50 and $150 (includes reception with performers).
may 18
Huehuetl Huehuetl (pronounced something like "whit-whit") is a family affair from Jalisco, Mexico, that uses legend, dance, music and ritual to tell the history of ancient Mayan, Teotihuacana, Olmeca and Aztec musical instruments. The Huehuetl adolescents teach children to make woodwinds, which they can play in the performance. Gates open at 4 p.m. for a festival with food, poetry and the music workshop. Performance 5:30 p.m. Centro Aztlan, 5115 Harrisburg Boulevard. To pre-register for the workshop call 926-8771. Free.
10K Walk 'N' Roll-A-Thon The AIDS charities aren't picky: For their 10K fundraising event, you can propel yourself through Sam Houston Park however you like: walk, pedal, skate, blade, roll, crawl or ride virtually any non-motorized contraption. You'll be encouraged by high school cheerleaders, the Gay Men's Chorus and the HSPVA Jazz Quartet. Proceeds benefit 14 local AIDS service organizations. 9:30 a.m. Sam Houston Park at the corner of Lamar and Bagby. Registration is free; call 629-WALK.
Matisse, Picasso and Friends This traveling show's impressionist masterworks are so fragile and sensitive to light that they've rarely been presented to the public since the Baltimore Museum of Art acquired them in 1949. The 120 selections from the Cone Collection include 50 prints, drawings and watercolors by Matisse and another 50 by Picasso -- plus 20 pieces by Cezanne, Degas, Renoir and van Gogh. Through July 13. Museum hours are 12:15-6 p.m. Museum of Fine Arts, 1001 Bissonnet, 639-7300. $3.
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A Denim Affair Seguin's own Janice Woods Windle wrote a best-selling novel, True Women, concerning the epic stories of Texas frontier women -- a book largely based on her own family's folklore. Tonight, she'll get to watch its metamorphosis into a CBS miniseries starring Dana Delaney of China Beach fame. True fans can join Windle at a watch party for part one of the TV event hosted by the Women's Fund for Health Education and Research. Put on your cowboy boots, pick out your favorite recipes and meet the true woman herself. 7 p.m. Rice Memorial Center, Rice University, 974-5570. $35.
may 19
Scott Gertner's First Anniversary Talent Search Rock star wannabes will have their five seconds of fame and a shot at $1,000. Bring your buddies -- they may rib you incessantly for your on-stage antics, but acts are judged based on audience response, so you'll need all the noisy friends you can round up. Gertner and his band go on at 9:30 p.m. tonight; the talent competition begins at midnight. Talent showcases continue all week. Midnight Tuesday and Wednesday; 10:30 p.m. and midnight Thursday and Friday. Each night's winners advance to Saturday's finals. Cody's in the Village, 2540 University Blvd., 520-5660. $6 to listen, $20 to play. Call (800) 701-2340 to register.
may 20
Houston Audubon Society Sanctuary Seminar Get away from it all without having to go too far: Paul Freed of the Houston Zoo takes you on a slide-show journey through the rain forests of South America. 7 p.m. Edith Moore Sanctuary Cabin, 440 Wilchester Boulevard. Reservations required. $5 donation suggested.
may 21
Picasso at the Lapin Agile If funnyman Steve Martin were to write a play, what would it be about? Of course: Albert Einstein and Pablo Picasso as twentysomethings, swilling beer together in a Parisian bar, commiserating about the nature of genius. 7:30 p.m. Runs through June 15. Alley Theatre, Large Stage, 615 Texas Ave. Call 1-800-259-ALLE for tickets and show times. $31-$43.


text:
This audiobook contains powerful Christian influences, love, family, and other related themes meant to make you smile!
Christian Lumberjack In The Old West, is a wonderful and emotional story about one man's journey in life and the old west, as he heads to Oregon to become a lumberjack. His story is told through a contemporary woman, a librarian in New York City, who discovers most of the man's journal in the dusty reaches of the library basement. Through his story - one of inspiration and hope - he lives on. His words end up playing a significant role in her own life, convincing her to become both a better Christian and a better human being.
©2013 Quietly Blessed and Loved Press (P)2013 Quietly Blessed and Loved Press
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text:
hi my name paul and i am new to this
This is what I have.
*San disk 8GB Micro SD HC MicroSD Memory Card 8 GB new - eBay (item 220442367167 end time Jun-26-09 14:07:23 PDT)
I've tried using the usb adapter for usb loader gx but it gives me the known error:wbfs partition not found.My lexar 2gb pen drive works fine.
I've also tried using the microSDHC alone with the SD adapter for usb/sd loader v1.5.However, it gives the same error.I've tried both cios38rev13a and cios36rev10.
Steps peformed:
formated card to fat32(only option available)
format to wbfs
transfered over iso's fine
error:wbfs partition not found for gx loader and loader v1.5
Any suggestions will be great.
hi my name paul and i am new to this
a suggestion?... Google it. (as i am unsure )
Last edited by Breakell20; 07-14-2009 at 04:18 AM.
Here to help in anyway shape and or form (or at least try, ive been out of the wii scene for quite a while)
If You Like What I Say, THANK ME!
No Mod Chip/Clip
4.0E With StartPatch 4.06 PAL
v386 (v24) Wii
LEH1 Serial! THE BEST!
Mario Kart : 0216-1986-4790
Wii Console : 6009-4439-3108-6643
PM me with yours if you add me
DE BLOB escaped and attacked my Signature!
Any more info on this. I want to buy an 8gb microSDHC for doing game backup ISOs. I currently have a 2gb but you have to then combine the multiple ISOs into one to burn it to a backup dvd. I would rather use a large enough memory card to just rip one ISO.


text:
How We Develop Preferences?29 Jun 2005 (Wed)
From Elearningpost, June 27, 2005:
"This is a wonderful explanation for why we prefer some items over others. This can have some learning design implications. The finding: We prefer items that we 'remember', rather than that we 'know'. So, what's the difference between 'remembering' and 'knowing'?
"When we 'know' something, we're completely aware of its existence, but we don't recall the specific instance when we learned of it. When we 'remember,' we're recalling a particular occasion."
"To put it simply, if we have story to tell about the item, we are inclined to prefer it. Not a bad marketing mantra if you think about it."
Strange. Wouldn't it be the other way round? When we like something, we are impressed by it and so we remember it? Could the researchers be inverting the symptom (remembering) and the cause (preference)? Read the rest of this entry »
- 5 Ways we consume info in Web 2.0 world
- Roundup#1: Microformats, 32GB SSD, UCLA-Moodle…
- Extreme Hallucinatory Daydreaming
- Permission Marketing – revisited
- Yay! First Lap Done!
- Virtual Conferencing Not Here Yet?
- Chinese Art Values Imitation?
Posted by J.K. in Cognitive, Problems, Psychology, Research | View Comments |


text:
Search continues to bolster the roster
FOXBOROUGH - Help could be on the way for the Revolution.
"We've made two offers over the weekend,'' coach Steve Nicol said after a 3-0 loss to Philadelphia last night. "One we thought was done, then at the final hour we find out the player's signed with someone else. That's happened on two or three occasions already, we've made offers, they've agreed to it, and at the final minute they go somewhere else. So, we're certainly trying.''
The Revolution have lost four foreign players (Ousmane Dabo, Didier Domi, Marko Perovic, and Ilija Stolica) since the start of the season. Dabo, limited to three appearances because of injuries, is expected to announce his retirement this week.
Still skidding The Revolution, 0-6-3 since May 14, matched a team record nine-game winless streak. In 1997 and '98, they compiled nine-game losing streaks (three matches ended in ties, but the Revolution lost in shootouts). In 2003, the team had a nine-game winless streak (0-5-4).
The Revolution had goal differentials of 6-22 in 1997 and 12-28 in '98 during the losing streaks. They were outscored, 12-19, during the '03 skein, rallying in the playoffs to reach the Eastern Conference final. They have a 7-15 differential on the current skein.
The result against the Union was the Revolution's worst home defeat of the season.
"We were flat before the game, we were flat to start out the game,'' goalkeeper Matt Reis said. "We never really got into the game. Towards the end, we were just pumping the ball up the field, and that's not necessarily going to work. We've got to figure out something and soon.
"I think we definitely have the guys to do it, it's just having everybody on the same page. Guys think we should be doing other stuff and guys think we should be doing this and that, and Stevie says, 'We're going to be doing this,' then we all have to do it. And, we all have to believe in it and we're not getting that.''
Reaching his goal Philadelphia defender Sheanon Williams scored on a breakaway for his first MLS goal. "Definitely a dream come true,'' he said. "Score in the 90th minute, home crowd, I couldn't ask for anything more.'' Williams grew up in Dorchester and played for the Greater Boston Bolts and Rivers School, and for the US Under 20 national team. He joined the Union after performing as a forward for the Harrisburg City Islanders in the USL. "I grew up here but I was really looking for any team to give me a chance,'' Williams said. "I was here but couldn't sign for [the Revolution] for MLS reasons. The coaching staff here in Philly has given me a great opportunity. It's really great they had the belief in me to give me the starting job and I'm not looking back, I'm going to keep moving forward.'' . . . Revolution supporters' groups in The Fort section behind the North goal staged a protest during the game. The groups, dressed in black, failed to produce their usual chants, then walked out of the stadium in the 17th minute. The supporters were protesting the arrest and detention of members during the Revolution-Chicago game last month.
Frank Dell'Apa can be reached at email@example.com.


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Moderators: Krisi, Vega, EC, Dixie
Oriani wrote:Hello!! Do you mean Chocolate?? Well I'm giving you this, If it's not what you're asking for, let me (us) know.
Try this and
You will need :
8 cups nonfat dry milk powder
1 package (15 ounces) instant chocolate drink mix
1-1/2 cups powdered nondairy creamer
1-1/4 cups confectioners' sugar
3 tablespoons instant coffee granules
1/4 teaspoon ground cinnamon
1 envelope unsweetened orange or raspberry soft drink mix
Chocolate Cups
1/2 cup semisweet chocolate chips
1 teaspoon shortening
Whipped cream in a can
What to Do
1. In a large bowl, combine the first seven ingredients; mix well. Store in an airtight container for up to 6 months.
2. For chocolate cups, in a microwave, melt chips and shortening; stir until smooth. With a small pastry brush or 1/2 teaspoon measure, spread chocolate mixture on the inside of 1-in. foil or paper candy cups. Place on a baking sheet. Refrigerate for 45 minutes or until firm. Just before serving, remove foil or paper cups and add whipped cream.
3. To prepare hot chocolate: For each serving, combine 1/3 cup mix and 1 cup boiling water; stir to dissolve. Place one filled chocolate cup in each mug; stir until melted.
Yield: 11 cups hot chocolate mix and 16 chocolate cups
I think this is what you're asking for, but I gave you more clues, even I'm not good at cooking or preparing drinks..!
serenella wrote:Well, in most Italian coffee bars you can find different kinds of hot chocolate (with cinnamon, coffee, bitter cocoa, caramel, nuts, etc.) but they are prepared using a powder mixture ready for use.
Personally, I prefer the real hot chocolate, made with pieces of chocolate, melted in hot milk and with a lot of whipped cream on it...
Yes, coffee with milk is very good too: every morning for breakfast I have a 'cappuccino'
Users browsing this forum: No registered users and 7 guests


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Prague, nice, luxury, furnished apartment 3+1 (140m²) for rent, Betlémská, Staré Město
Beautiful, luxury apartment 3 +1 for rent on the street Betlémská in Prague 1 – Old Town. This apartment with total area of 140 square meters is located in the 3rd floor of a brick building with an elevator. The apartment is furnished, the furniture is antique. In the living room is a sofa. The apartment has two bedrooms. One bedroom has a double bed, the other two single beds. The kitchen is fully equipped – fridge, freezer, ceramic hob and dishwasher. There are two bathrooms – one bathroom with tub and toilet, in the second bathroom is a shower, toilet and there is a washing machine. The apartment has television, satellite, Internet connection. Possible to rent a garage for an extra fee. The house is situated in a beautiful location, close is famous Charles Bridge. Not far from the house is a metro Národní třída and tram stops.
80 000 CZKper month
|+ utilities 7000,-
|Offer ID
|Betlémská, Praha 1 - Staré Město
|very good
|4th floor
|Usable area
|140 m²
Contact a broker
Markéta Svitáková
broker - long term rentals
Office: Prague central
Mobile phone: +420 775 666 414
Thank you for your interest
We are currently processing your request. Our real estate agent will contact you within 24 hours. You can also use our free line 800 800 505.
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In the couple of last months, I have got to know tens of real estate brokers and real estate agencies, maybe even more, and you are the only one who can be .... as a "really good real estate broker".More info
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- sophisticated advertising all around the world, your real properties will be seen
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- large database of inquirers
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- free customer line 800 800 505
Frequently asked questions
- Does the price of the rental include service charges?
- May I make the rented flat my permanent residence?
- For how long period is the lease contract signed, is it possible to enter into a lease for an indefinite period and how long is the period of notice?
- Why should we enter into a reservation contract when buying a real property?
- What comprises the brokers´ fee of Home Sweet Home in the process of selling your real property?
Ask us
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We will contact you at the earliest convenience.


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Media Myth: Networks Stick to Warming Theme Despite Avalanche of Chilling News
Its cold outside. Just ask residents from Florida to New England and they will tell you its been a brutal winter. Despite bone-chilling records for cold and snow, the networks continue to warn about global warming and ignore scientific claims that the Earth is facing a cool spell.
A Letter from a Working Person
Dear Chris Matthews, build your own business and see if it feels like work.
Here Lies a New Administration - Again and Again
Media ignore the excesses of Pinocchio politics.


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New to America
HeadBed is the all new, unique and truly innovative Head and Neck Support Cushion, finally solving the problem of client discomfort at the shampoo bowl.
The HeadBed head and neck support cushion will provide ultimate comfort and safety for your client at the shampoo bowl. HeadBed USA is new to market and has been designed to fit the standard USA shampoo bowl.
Suitable for USA shampoo and backwash bowls.
HeadBed is a small platform that is built into a rubber cushion that sits on a shampoo bowl. It assists in placing clients in the optimal position for all shampoo bowl services as it is designed to support the head at its heaviest point, the occipital bone.
As a result your clients can lay back, relax and enjoy pre-service shampoos, treatments and post-treatments without discomfort or pain.
HeadBed comes in 2 models. One to specifically fit the USA style shampoo bowl, and one designed for the backwash shampoo bowl.
Key Benefits:
- Medically endorsed
- Hygienic, soft and durable
- It will provide your clients with comfort and safety
- Our design supports and protects the neck from injury
- Assists with hairdressers safety and well-being
- Meets Salon Occupational Health and Safety Regulations
- It will compliment salon procedures and safe work practices at the shampoo bowl
- Ensures comfort and safety whilst applying a range of scalp massage movements
- Makes colour removal very easy
- Your clients, of all ages, will finally be comfortable and safe at your shampoo bowl!


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Get Free Weekly Newsletter
Living with Chronic Pain
Store Featured Item
How healthy is your Relationship?
Answer each of these 10 questions to get an idea on the state of your relationship. It is not a comprehensive assessment or diagnostic instrument but a bit like a "report card" of how you may be doing. The good news is that if you and your partner want to improve any of these skills you will be able to. After you complete the questions you will see the score and what it means.


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PORTLAND, Ore. – From learning he'd start his first MLS game on opening night, to nearly giving up an own goal and then finally scoring one of his own, Portland Timbers rookie defender Andrew Jean-Baptiste had quite an evening.
To top it off, Jean-Baptiste's mother flew in from New York to see her son make his pro debut. She ended up watching him help lead the Timbers past the Philadelphia Union in a 3-1 victory in front of a rambunctious, sold-out crowd on a rainy Monday night at JELD-WEN Field.
"It's really exciting," Jean-Baptiste said. "I'm not going to lie, it's really exciting. Knowing that 20,000 people saw my first MLS goal is very exciting for me."
HIGHLIGHTS: POR vs PHI
Jean-Baptiste, drafted by Portland in the first round out of UConn in this year's SuperDraft, started in place of newly acquired Colombian Hanyer Mosquera, who was unavailable because of a delay in his international transfer certificate.
And for a few minutes, it looked like the rookie's inexperience would cost the Timbers.
A free kick by Philadelphia's Gabriel Gomez sailed through the defense and nicked off Jean-Baptiste before landing in the back of the net to give the Union a 1-0 lead in the 51st minute.
It was originally ruled an own goal, but later credited to Gomez. Either way, Jean-Baptiste was quick to own the defensive lapse.
"You're going to make mistakes," Jean-Baptiste said. "Every defender has probably scored an own goal on their team, and the first thing they want to do is get it back for their team. And that was the first thing I thought."
The sting was quickly alleviated when the big central defender found himself on the end of a set piece by midfielder and team captain Jack Jewsbury.
Jean-Baptiste slid under the curling pass and headed it past Union goalkeeper Zac MacMath to even the score in the 54th minute.
"It's pretty heavy," Jean-Baptiste said of his equalizer. "It's very heavy actually. It feels great. I just hope that all my friends and family back home were watching the game."
Timbers head coach John Spencer said he didn't hesitate when penciling Jean-Baptiste's name in the starting 11.
"I've got no problems with putting him in," Spencer said. "I think you've got to look at it and say you've got to worry about the things you can control. And we could control putting him in to start, and he performed. We don't expect to put him in to play badly. And players don't try to play badly, and they try to play well every week. And, as I say, I thought he played particularly well."
Newly acquired Designated Player Kris Boyd and second-year midfielder Kalif Alhassan added the remaining goals. But Jean-Baptiste's night was sealed as one he'll never forget, starting with the low of allowing a goal and then the high of celebrating with the Timbers Army after notching his first professional tally.
"It's a good experience to have now," he said. "To know what crowd I'm going to play in front of and what the teams are like around the league. It's good for my comfort level."
Dan Itel covers the Timbers for MLSsoccer.com. Email him at email@example.com
Spencer celebrates 3 goal 2nd half


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The euro zone had a much smaller than expected trade gap in December as imports shrank more quickly than exports, data showed, pointing to falling domestic demand as the euro area sinks deeper into recession.
The European Union's statistics office, Eurostat, said the unadjusted trade deficit in the 15 countries using the euro in December was 700 million euros against a 3.9 billion gap the year before and market expectations of a seven billion shortfall.
Year-on-year imports fell five percent in December while exports shrank two percent, Eurostat said.
"Exports and imports are slowing very sharply, and it's consistent with ... demand from import and export markets plunging," said Martin van Vliet, economist at ING.
"I'd expected the trade balance to widen a bit, but the dramatic import figure suggests that aggregate demands in the euro zone is very weak, and that's not a positive sign at all. The euro zone is in a deep recession," he said.
For the whole 2008, the single currency area had a trade gap of 32.1 billion euros against a surplus of 15.8 billion in 2007. "Looking ahead, I think things are set to go from bad to worse, given that the surveys of export orders just point to absolutely huge falls in annual export growths over the coming months," said Ben May, economist at Capital Economics.
"It looks very likely that exports will be a sharp drag on the economy this year," he said.
Seasonally adjusted, the December trade deficit was 300 million euros against a four billion gap in November as imports fell 3.9 percent month-on-month and exports fell 0.9%.
Detailed data for December was not yet available, but figures for the January-November period showed the biggest driving force behind the deficit was still the shortfall in energy trade which widened to 286 billion euros from 210.9 billion in the 11 months of 2007.
"At least though, the sharp retreat in oil prices from a record high of $147 per barrel in July to recently trade under $40 per barrel will help the euro zone's trade balance," said Howard Archer, economist at IHS Global Insight.
The euro zone's trade deficit with energy exporter Russia widened in January-November to 34.3 billion euros from 26.9 billion a year earlier. (Reuters)


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Members of Youngstown State University sororities and fraternities gathered Monday evening to remember Jamail Johnson and those wounded in weekend shootings near campus.
More than 50 students met at Peaberry's Caf in YSU's Kilcawley Center to make purple and gold ribbons to pass out and wear. Purple and gold are Omega Psi Phi fraternity colors, and the shootings took place in a house where many of the brothers lived.
"He was just real cool," sophomore Lelia Clark said of Johnson. "I'm surprised it even happened at that house. I always felt safe there."
Clark and others found out about Sunday's events through e-mail and text alerts from the university as well as word of mouth.
"I really appreciated how fast they got the e-mail out," said senior Melania Vlad. "I think it did a lot to clarify things."
Nearby in the Chestnut Room of the Kilcawley Center, dozens more students gathered for a prayer service sponsored by the Collegiate, Black and Christian organization, or CBC.
CBC administrator and senior Sasha Hill said she felt students needed prayer after the weekend's events.
"People need to know that in the midst of tragedy, God is still present," she said. "We just want to offer something to the students."
Hill had students pray with one another during the hour event. They stood in small circles with their eyes closed, holding hands and whispering prayers.
Hill said CBC will likely have more events in response to the shooting throughout the week.
Sign up for our newsletter to receive daily news directly in your inbox.


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Rush hour traffic heading into Sleaford was at a standstill this morning (Friday) after firefighters were called out to assist railway workers clean the tracks at Southgate level crossing.
Apparently dirt and grit, especially salt from gritters, had interfered with the electrical sensors on the line, confusing the system into thinking a train may be on the crossing when it wasn't.
In turn this caused the crossing gates to fail and traffic was tailed back out of town.
A firecrew was called to assist Network Rail staff flush out the dirt from between the rails with a high pressure hose.
This was quite a common occurrence according to one Network Rail official.
The track was finally cleared just after 9am.


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Obama continues economic push in Missouri
President Barack Obama delivered his second round of remarks on the economy at the University of Central Missouri on Wednesday. Obama pledged his commitment to the American dream and pushed for Republicans to work with him moving forward. Watch his full remarks in the video above.


text:
Originally Posted by titus
Sound: great sound and together with the dark environment it creates a creepy feeling.
leveling: every level you get 10 points wich you can use for buying a new spell or/and improving your stats.
encountered a first puzzle. ony solved a part. needed to find a code for opening a gate and the code was written in a book you find if you clear the dungeon. But the gate next to that one was impossible to open for me
VPeric were you able to open it?


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Motorists are being advised of continuing road works in Redhill.
Traffic lights will be in operation at the works, ongoing from Monday, May 12, in Brambletye Park Road and Knighton Road.
The works are part of Sutton and East Surrey Water's ongoing multi-million pound mains renewal and replacement rolling programme.


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The Palo Alto City Council is poised Monday to enact water-use restrictions to comply with a new drought-related mandate.
At its July 15 meeting, the State Water Resources Control Board approved emergency regulations to ensure that water agencies, their customers and residents increase conservation in urban settings, with a specific emphasis on outdoor irrigation, according to a city staff report.
The decision prohibits certain actions regarding water use and also requires suppliers, such as the city of Palo Alto, to implement portions of their water shortage contingency plans that apply to outdoor irrigation.
The city's plan covers two of the prohibited actions: irrigating landscaped areas to the point of runoff and using hoses without shut-off valves. To fully comply with the mandate, a resolution the council is set to vote on would also bar the washing of sidewalks and driveways, as well as require a re-circulating system if potable water is used in an ornamental fountain.
Violations could result in fines of up to $500 per day for individuals, according to the report. Cities could also be penalized $10,000 per day if they don't implement the relevant sections of their water shortage contingency plans.
Palo Alto gets all of its potable water from the San Francisco Public Utilities Commission. To date, the utility has asked its wholesale customers to voluntarily curtail water consumption by 10 percent. The city responded by cutting usage by about 17 percent between February and June, compared with the same period in 2013, according to the report.
However, amid one of the worst droughts in recent memory, water use in May actually rose by 1 percent statewide, compared with a 2011-13 May average, according to recent survey.
The City Council meeting is scheduled to begin at 7 p.m. in the Council Chambers of City Hall, 250 Hamilton Ave.
Email Jason Green at email@example.com; follow him at twitter.com/jgreendailynews.


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Boys Bedroom Accessories
If you are shopping for a boy's bedroom here is our range of accessories neatly sorted in to one place for you.
Showing page 1 of 1 - Show 28 products per page
The allkids.co.uk range of boy's bedroom accessories allow you to easily turn your kid's bedroom into the ultimate lad pad. These products have been specifically chosen so you can customise your boy's bedroom quickly and easily, at a very low price point, taking the expense away from keeping up with the latest trends.
Furthermore, our boy's accessories range is only made up of products we know boys love to use, and these products are available in a wide variety of themes, colours and styles. The range encompasses bean bags, blankets, cushions, lighting, rugs and wall art – meaning your boy and his friends can enjoy socialising, chilling out, playing and maybe even studying in the bedroom! As an added benefit, all of our accessories can be matched to our wide range of boys bedding, allowing you to easily co-ordinate the room.
One of the most popular categories of products in the range has to be our collection of bean bags. Popular with boys from 18 months to early teens, these beanbags make an excellent addition to a kid's bedroom, allowing them and their friends extra seating space which looks great. If you have any questions about the range, or need a hand finding the right bedroom accessory, just get in touch.


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Neoseeker : Videos : Walkthroughs : Maisonette Management 6
Grand Theft Auto IV: The Ballad of Gay Tony
- By Rockstar North for PC, PS3, Xbox 360
Maisonette Management 6
Register as a member to subscribe comments.COMMENTS
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Trailer Info
|From: KnightRazor
|Apr 6, 2014
|Category: Walkthroughs


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Upstream / Downstream
Explore pathways related to this product.
To Purchase # 15102S
|20 µl (2 western blots)
|100 µl (10 western blots)
Find answers on our FAQs page.
PhosphoSitePlus® Resource
- Additional protein information
- Analytical tools
cGAS (D1D3G) Rabbit mAb #15102
Western blot analysis of extracts from various cell lines using cGAS (D1D3G) Rabbit mAb.Learn more about how we get our images
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human cGAS protein (hcGAS-Myc; +), using cGAS (D1D3G) Rabbit mAb.Learn more about how we get our images
Gallery: cGAS (D1D3G) Rabbit mAb #15102
Western Blotting Protocol
For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
- 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
- 10X Tris Buffered Saline (TBS): (#12498) To prepare 1 L 1X TBS: add 100 ml 10X to 900 ml dH2O, mix.
- 1X SDS Sample Buffer: Blue Loading Pack (#7722) or Red Loading Pack (#7723) Prepare fresh 3X reducing loading buffer by adding 1/10 volume 30X DTT to 1 volume of 3X SDS loading buffer. Dilute to 1X with dH2O.
- 10X Tris-Glycine SDS Running Buffer: (#4050) To prepare 1 L 1X running buffer: add 100 ml 10X running buffer to 900 ml dH2O, mix.
- 10X Tris-Glycine Transfer Buffer: (#12539) To prepare 1 L 1X Transfer Buffer: add 100 ml 10X Transfer Buffer to 200 ml methanol + 700 ml dH2O, mix.
- 10X Tris Buffered Saline with Tween® 20 (TBST): (#9997) To prepare 1 L 1X TBST: add 100 ml 10X TBST to 900 ml dH2O, mix.
- Nonfat Dry Milk: (#9999).
- Blocking Buffer: 1X TBST with 5% w/v nonfat dry milk; for 150 ml, add 7.5 g nonfat dry milk to 150 ml 1X TBST and mix well.
- Wash Buffer: (#9997) 1X TBST.
- Primary Antibody Dilution Buffer: 1X TBST with 5% nonfat dry milk; for 20 ml, add 1.0 g nonfat dry milk to 20 ml 1X TBST and mix well.
- Biotinylated Protein Ladder Detection Pack: (#7727).
- Prestained Protein Marker, Broad Range (11-190 kDa): (#13953).
- Blotting Membrane and Paper: (#12369) This protocol has been optimized for nitrocellulose membranes. Pore size 0.2 µm is generally recommended.
- Secondary Antibody Conjugated to HRP: Anti-rabbit IgG, HRP-linked Antibody (#7074).
- Detection Reagent: SignalFire™ ECL Reagent (#6883).
B. Protein Blotting
A general protocol for sample preparation.
- Treat cells by adding fresh media containing regulator for desired time.
- Aspirate media from cultures; wash cells with 1X PBS; aspirate.
- Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice.
- Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).
- Heat a 20 µl sample to 95–100°C for 5 min; cool on ice.
- Microcentrifuge for 5 min.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#13953, 10 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
- Electrotransfer to nitrocellulose membrane (#12369).
C. Membrane Blocking and Antibody Incubations
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
I. Membrane Blocking
- (Optional) After transfer, wash nitrocellulose membrane with 25 ml TBS for 5 min at room temperature.
- Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
II. Primary Antibody Incubation
- Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the product datasheet) in 10 ml primary antibody dilution buffer with gentle agitation overnight at 4°C.
- Wash three times for 5 min each with 15 ml of TBST.
- Incubate membrane with Anti-rabbit IgG, HRP-linked Antibody (#7074 at 1:2000) and Anti-biotin, HRP-linked Antibody (#7075 at 1:1000–1:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
- Proceed with detection (Section D).
D. Detection of Proteins
Directions for Use:
- Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST.
- Prepare 1X SignalFire™ ECL Reagent (#6883)by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.
- Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Western Blot Reprobing Protocol
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
- Wash Buffer: Tris Buffered Saline with Tween® 20 (TBST-10X) (#9997)
- Stripping Buffer: To prepare 100 ml, mix 6.25 ml of 1M Tris-HCl pH 6.8, 10 ml of 20% SDS and 700 μl β-mercaptoethanol. Bring to 100 ml with deionized H20. Make buffer fresh just prior to use.
B. Protocol
- After film exposure, wash membrane four times for 5 min each in TBST. Best results are obtained if the membrane is not allowed to dry.
- Incubate membrane for 30 min at 50°C in stripping buffer (with slight agitation).
- Wash membrane six times for 5 min each in TBST.
- (Optional) To assure that the original signal is removed, wash membrane twice for 5 min each with 10 ml of TBST. Incubate membrane with LumiGLO® with gentle agitation for 1 min at room temperature. Drain membrane of excess developing solution. Do not let dry. Wrap in plastic wrap and expose to x-ray film.
- Wash membrane again four times for 5 min each in TBST.
- The membrane is now ready to reuse. Start detection at the "Membrane Blocking and Antibody Incubations" step in the Western Immunoblotting Protocol.
posted June 2005
revised June 2016
|Western Blotting
cGAS (D1D3G) Rabbit mAb recognizes endogenous levels of total cGAS protein.Species Reactivity: Human
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala19 of human cGAS protein.
Cyclic GMP-AMP synthase (cGAS, MB21D1) is an antiviral enzyme that produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (1,2). The cGAS protein acts as a cytosolic DNA sensor that binds DNA and produces the cGAMP second messenger from ATP and GTP (1,2). cGAMP binds to and activates STING, a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1-4). STING is ubiquitously expressed and found predominantly in the ER (3). Following activation, STING translocates with TBK1 to perinuclear endosomes (5). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (3-5).
1. Sun, L. et al. (2013) Science 339, 786-91.
2. Wu, J. et al. (2013) Science 339, 826-30.
3. Ishikawa, H. and Barber, G.N. (2008) Nature 455, 674-8.
4. Zhong, B. et al. (2008) Immunity 29, 538-50.
5. Ishikawa, H. et al. (2009) Nature 461, 788-92.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Tween is a registered trademark of ICI Americas, Inc.


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For the state legislature's 2016/2017 session, Senator Sweeney was unanimously confirmed and sworn in as President of the New Jersey Senate, the highest-ranking position in the state's legislature. As the
Senate President, Senator Sweeney plays a key role in setting the Senate's agenda and building coalitions.
Assemblyman Burzichelli was sworn in as the Deputy Speaker of the New Jersey Assembly and is the chair of the Assembly Appropriations committee, vice chair of the Assembly Budget committee, a member of the Tourism, Gaming, and the Arts committee and the Legislative Services Commission, and he serves on the Joint Budget Oversight Committee.
Assemblyman Taliaferro was sworn into the New Jersey Legislature in January of 2015. He serves as vice chair of the Assembly Agriculture and Natural Resources Committee and is a member of the Assembly Education Committee.
- Agriculture & Environment
- Consumer Affairs
- Economic Development
- Education
- Health
- Labor
- Property Tax Relief & Government Reform
- Public Safety
- Seniors
- Transportation
Please click here to view legislation sponsored by Senator Sweeney, Assemblyman Burzichelli, and Assemblyman Taliaferro during the most recent legislative session.


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Shawn Marion: This is painful
While being competitive and losing close games like the Mavericks have done four times in a row now is far preferable to the 30-point blowouts they were suffering 10 days ago, the frustration level continues to grow.
Working hard and getting no payoff is getting old fast.
"Words can't even express it right now," said Shawn Marion after the 99-93 setback to the Clippers Wednesday night. "This is painful to be losing like this when we're so close. When we get right there, we usually do something to mess it up. We played a solid game."
The Mavericks had chances even after they lost their 10-point lead by sleepwalking through the final 90 seconds of the third quarter and falling behind by seven with under five minutes to play.
They cut it to three, then two and were still only down 97-93 when Darren Collison got a terrific open 3-pointer from the corner. When that one missed with 1:35 to play, the Clippers got two tough offensive rebounds by former Maverick Caron Butler to sseal the deal.
"We knew they were going to make a run," Nowitzki said. "Then we fought back. I liked how we responded to their run and came back and had our chances. We were down four, I got double-teamed and swung the ball and DC had a great look at a three to cut it to one. And then down the stretch, you got to get a rebound. You can't give two offensive rebounds up in the last minute when you get good stops.
"Frustrating, there's no other word. But we're not quitters. The coaching staff is not quitters. So we're going to keep on pushing and they'll keep on teaching."


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Brown Sugar
Flavored Rolling Papers
Natural Brown in Color,
Imported from Spain, Brown Sugar Cigarette Rolling Papers
provide a sweet, rich taste to your smoke.
Available in 6 Flavors:
Peach, Chocolate-Mint, Strawberry,
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and Island Blend (sweet coconut)
Each packet contains 20 papers.
Any Brown Sugar Six-Pack (Your Choice of 6 Flavors): $12.00
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Contention Resolution and Channel Estimation in Satellite Random Access Channels
The authors consider the Random Access CHannel (RACH) of a multi-beam satellite system with universal frequency reuse. They benefit from the spatial diversity and the strong directivity of multiple antennas at the satellite to resolve contentions and perform channel estimation. They propose two approaches to detect active Satellite Terminals (ST) and estimate the channel coefficients of some or all the colliding STs: Grid Reduction (GR) and Successive Channel Cancelation (SCC) algorithm. The performance of the proposed approaches is assessed by numerical results and compared with the conventional satellite RACH system.
Provided by: Institute of Electrical & Electronic Engineers Topic: Networking Date Added: Aug 2012 Format: PDF


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Our Building
Philadelphia is enriched by centuries-old tradition and history, looking to the past as a guide to the future, whose footsteps we'd like to follow as modern-day stewards and keepers of this ever-evolving city. And part of this evolution is Rittenhouse 1715 Square, where the city's past, present and future merge. The original structure was built in 1903 as the carriage house for the Alfred C. Harrison residence, for which he commissioned Philadelphia's then-most prestigious architectural firm, Cope & Stewardson, to design the house along with his residence and the Alfred C. Harrison building. Notably distinguishing the carriage house from the other buildings are the numbers above the stable doors at the entrance and the balconies with wrought iron decals. An amazing structure - quite refined!
Yes, we're small, but that works for us. We're relatively old as well - 110 to be exact. Sure, we're not as old as London, Athens or Rome. But old enough in modern history, a history we cherish, along with the wisdom of our country's revolutionaries. And we're a little bit quirky, too. After all, we did transform a turn-of-the-twentieth-century carriage house into a four-star boutique hotel - and every inch with five-heart service!
Life-long friends beginning in childhood, Walter Cope and John Stewardson were destined for immortality as they embarked on their architectural venture. Among their designs still extant are the University of Pennsylvania Museum (done in collaboration with the architectural firm Miles and Day); the quadrangle dormitories at the University of Pennsylvania; Blair Hall at Princeton University; and Rockefeller Hall at Bryn Mawr College. Their designs were influenced by three architectural styles: Collegiate Gothic for university buildings and Italian Renaissance and Romanesque for residential and industrial buildings.


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For those of you who have been emailing in wondering about the Bella's engagement ring being sold by Twilight Teez, this is the latest info regarding the ring that was supposed to go on sale today.
We will need to push our Bella's Engagement Ring one more time. The new launch date and time is as follows:
July 20, 2008 at 12:00:00 noon (PST).
For your inconvenience, we will be offering a 10% discount on the ring purchases from now until July 27, 2008.
-Brian Schade
Chief Technology Officer
Twilight Teez, LLC
You can read the full explanation here.


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ROME, Oct. 25 (UPI) -- The European Commission said it was waiting to hear from Rome how Italy will manage its financial troubles.
The answer to that may not be right around the corner, ANSA reported Tuesday.
Some say the coalition government of Silvio Berlusconi is on the verge of collapse, as the premier tries to pass austerity measures that eurozone partners have demanded.
A spokesman for European Commission Jose Barroso said, "EU is awaiting a letter from Berlusconi with the specific measures Italy intends to adopt and up to now it has not had any indication."
In Rome, however, an emergency Cabinet meeting ended with no formal agreements.
''We are negotiating but it seems to me that there is this hypothesis,'' said Infrastructure Minister Altero Matteoli, referring to a hypothesis presented to him by a reporter that the Berlusconi's government could collapse.
After blocking Berlusconi's pension reform plan, Northern League party leader Umberto Bossi said, "there is room for negotiations."
Progress, however, may come slower than it did in September, when the government approved a $75 billion austerity package that was expected to put the budget back in balance in 2013, ANSA said.


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At a glanceYour little one will feel like she can take on the world in the Mothercare Superbaby All In One. This lovely piece is pretty and perfect for miniature superheroes.
Features and benefits for Mothercare Superbaby All In One
- 100% soft cotton
- Neck envelope for easy dressing
- Machine washable for easy care
- Nickel free poppers prevents skin irritation
Super-cool girls will love the Mothercare Superbaby All-In-One.
The Mothercare Superbaby All-In-One is made from super soft cotton with a popper opening for easy dressing and nappy changed - all the poppers are also free from nickel, perfect for keeping her comfortable.
The enclosed feet of the all-in-one will keep little toes warm and cosy, whilst the open long sleeves give her plenty of room to stretch and wriggle - just right for superheroes in training!
Don't Forget...
... that superbabies need to travel in style so check out our great range of prams and pushchairs.
Fabric composition: 100% Cotton
Care instructions: Machine Washable
Tumble Dry


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Read the World. Write a Way.
A degree from the Department of English is exceptional not just in terms of the excellence of our work but in the unique experiences fostered by the exploration of literature, rhetoric and writing. Students and faculty are committed to a variety of intellectual and creative pursuits, working together and independently to challenge and exceed expectations.Learn More


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Rebekah Jordan (AKA Kinky, Rebekah Keeley, Kinki, Rebekah, Rebecca, Poppy, Shawna, Karen) is a hot English babe who was born in Scunthorpe on April 11, 1981. The 5'4" vixen started her career in the adult industry as soon as she possibly could, at the age of 18. She took the world by storm, and we weren't ready for what this B-cupped beauty had to show us, especially when it came time for her to get down with some backdoor action. With her bright smile and her figure measuring 34-23-31, she really works her fans into a frenzy whenever they start stroking to her clips.
Rebekah is the type of porno babe who is always looking for something new to try. When she teams up with other adventurous performers, magic happens in between the sheets. She stars with Trent Tesoro and Ashley Blue in a scene for UK Student House 8 that you'll never forget. While she's riding Trent's fat cock, Ashley makes sure that he's pounding her as hard as he possibly can by getting him to push on the hot blonde's shoulders. The hot threesome never gets a moment to breathe from the time that the cameras start rolling, until the pop shot is sprayed all over the set. Rebekah holds her own with the legend of smut. It's no wonder that sometimes she's billed under the name Kinky.


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Neuroscience and the Law
Application for 2 hours of CLE ethics credit pending
Please join us as New York Times best-selling author, Guggenheim Fellow, and neuroscientist David Eagleman, PhD speaks on the practical applications of neuroscience to the law. Advances in brain science provide tremendous insight into decision making, revealing different strategies for lawyers in the civil and criminal contexts. Advances in brain science are also calling into question traditional concepts of culpability. As seen and heard on TED, NPR, PBS, CNN and the Colbert Report, Dr. Eagleman is a dynamic speaker who can translate the profound implications of a complex science for the layman and lawyer alike.
When: TBA 2017
Tickets: $45 in advance, $55 at the door
All proceeds of the event will benefit the Center for Science and Law, which brings together neuroscientists, legal scholars, judges, practitioners and policy makers with the goal of constructing a legal system with higher utility and lower cost.


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Jaspersoft Extends Leadership in Embedded Intelligence
Jaspersoft 5.5 Introduces New Analytics Functionality, Enables Users to Gain Actionable Insight From Data In Minutes
Oct 29, 2013, 09:00 ET from Jaspersoft Corporation
SAN FRANCISCO, Oct. 29, 2013 /PRNewswire/ -- Jaspersoft, the intelligence inside applications and business processes, today announced the general availability of Jaspersoft 5.5, its flagship business intelligence platform. This new version advances the platform's existing embedded analytics functionality with new visualizations and increased performance.
Jaspersoft 5.5 includes new data visualization capabilities for quicker, more interactive insight into key data trends, a new homepage for one-click access to tutorials and samples, pre-built report templates to speed the development and quality of reports, enhanced cluster-aware caching for high availability, and the general availability of Jaspersoft Studio an Eclipse-based, full re-write of the iReport Designer, the company's signature reporting tool.
"This release marks major advancements to the industry's most powerful embedded analytics offering," said Karl Van den Bergh, vice president of product and alliances for Jaspersoft. "It's tailored to the realities of today's application landscape, where developers need to be able to easily embed analytics and rich visualizations in order to differentiate their offering and better satisfy their customers' needs. What's more, allowing developers the option to purchase such a platform on an hourly basis via our AWS partnership enables them to quickly realize value in an extremely cost-effective manner.
"One of the next evolutions in BI is the display of information at the right time and the right place through embedded reporting and analytics. We expect to see more applications improve and update their existing embedded reporting technologies to address new user requirements and provide a competitive edge," said Krishna Roy, BI and analytics analyst with The 451 Group. "Jaspersoft 5.5 has made some key self-service reporting and analytic improvements yet has done so with an embedded architecture and market in mind."
"Jaspersoft has always been a solid foundation for our reporting requirements," said Shabbir Mohammed, Principle BI Architect with Sabre Airline Solutions. " The new functionality in version 5.5 takes that a step further. New advancements in analysis and visualization tools enable me and my team to do more with our data than ever before. This is especially true when it comes to being able to easily visualize complex data in a straightforward manner that can better influence business decisions."
Learn More Today!
- See why more than 130,000 organizations have chosen Jaspersoft
- Visit http://community.jaspersoft.com for more information about the Jaspersoft open source community
- Follow @Jaspersoft on Twitter to stay up-to-date on all of the company's latest news and product information
About Jaspersoft
Jaspersoft empowers millions of people every day to make better decisions faster by bringing them timely, actionable data inside their apps and business processes. Its embeddable, cost-effective reporting and analytics platform allows anyone to quickly self serve and get the answers they need and scales architecturally and economically to reach everyone. Thanks to a community that is hundreds-of-thousands strong, Jaspersoft's commercial open source software has been downloaded millions of times and is used to create the intelligence inside hundreds of thousands of apps and business processes. Jaspersoft is a privately held company with offices around the world. For more information visit http://www.jaspersoft.com and http://community.jaspersoft.com
Media Contacts:
Nolan Necoechea
LEWIS PR for Jaspersoft
+1 415 432 2452
SOURCE Jaspersoft Corporation


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Retrax Pro Tonneau Cover 40372
- Our Price: $1,799.00
- Shipping:FREE SHIPPING
- Part Number: 40372
- Rating: (37)
Pro Tonneau Cover
- Part #: 40372
- Standard Rails without Stake Pocket Cutouts
$1,799.00Free Shipping
Select your vehicle details above to ensure item compatibility.
- The Retrax Pro Retractable Tonneau Cover is crafted from quality aluminum for lightweight, easy-to-handle protection
- Reliable and stylish protection for your truck bed
- Front canister is flush with the retracting cover for a dependable seal and true low-profile looks
- Cover opens and closes smoothly with just one hand
- Virtually zero-maintenance design - no springs or other moving parts to freeze, jam, or fail
- Locking design provides extra security for your gear, and locks in any position
- Compact storage canister measures approximately 11" square, keeping most of your bed space free
- Spiral track system prevents the cover from touching itself as it retracts
- Lifetime Warranty
When it's security, flexibility, and convenience you want, look no further than the Retrax Pro Retractable Tonneau Cover. This cover provides a reliable barrier of protection against weather, wear, and theft, and it's remarkably easy to use. Plus, its storage canister sits flush against the cover for a sleek, ultra-low-profile look.
Retrax Pro Tonneau Covers are crafted from durable, strong, and lightweight aluminum slats. This aluminum is tough enough to resist the hazards of rugged use and stand up to impacts from pelting hail. Automotive-grade weather stripping provides superior protection against rain and debris, and the cover's glossy Black Powder Coat finish adds a smooth finished-off look that lasts.
The Retrax Retractable Tonneau Cover provides instant truck bed access with the push of a button. The cover glides open and closed with one hand, without any snaps, tarps, or springs to fail or maintain. Its secure key lock system offers extra protection against theft. Plus, the cover locks in any position, so you can secure oversize cargo or partially cover your bed while hauling a fifth-wheel trailer.
Cover your truck bed the right way with the reliable, durable, and sleek Retrax Retractable Tonneau Cover. Backed with a Lifetime Warranty.
Retrax 40372 fits:
- 2009-2014 Ford F-150
Retrax Pro Tonneau Cover Customer Reviews
Overall Rating:5.0 of 5
Average Install Time: 1-2 hours
Showing 3 of 37 reviews. See All Reviews.
Great product. Everyone comments on it, often with some jealousy and stating that it's their next cover. I screwed up and ordered the wrong size the first time. The people were excellent to deal with. The day they received the shipment back with the wrong cover (which they paid for) they sent the correct one out. I couldn't be more satisfied with the product and service I received. Now I can lock stuff in the bed without worries.
Install Time: 30-60 minutes
Great product, easy to install, easy to use.
Install Time: Under 30 minutes
Only complaint at all about assembly and installation might be the nonchalant description of drain holes location, would it be that hard to do the research and describe drain holes for model and year and include a note in the model specific hardware box included? I was drilling with the hope of not damaging something and not even sure the area I was drilling was correct, it was, but it was by sheer luck.
Install Time: 1-2 hours
Showing 3 of 37 reviews. See All Reviews.


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It's a continuance of what our buddy Wade said is a "ridiculous Russian-doll situation" at Hummer. Following up on a report earlier this year indicating Hummer was considering a Jeep Wrangler competitor, dubbed H4, Automotive News says the GM brand plans to show off a conceptual version of that very truck at the Detroit show in January (no, the picture is a fakie). Production, AN, says, is assured for the 2010 model year, though there's still no word on which architecture the H4 would be built (no Daewoo-built front-driver, that's for sure). Word is, it'll have more serious off-road capabilities than its H2 and H3 siblings. And by that, we mean "some."
GM will unveil H4 concept at Detroit auto show [AutoWeek]
H4 for 2009: A Smaller Hummer on the Way? [internal]


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I was not planning on posting a traditional "2010 in review" post... until I saw that I had made 99 posts in 2010... and that it would be only appropriate to post once more to make that a round 100. :-)
2010 was most certainly a year to remember. A rollercoaster of emotions - of sorrow and celebration and love. Amidst all of it - I have MUCH MUCH MUCH to be thankful for.
So here it goes...
- I visit with the doctor for the first time to discuss fertility treatments. We agree that due to my outrageously long cycles, clomid is the best bet. I am hopeful/excited for the first time in months!
- Jordan's band, Those Eighty Acres, releases their first CD!
{my rockstar husband}
- My good friend, Mandy, gives birth to Lucy Joan and we are all oh-so in love and have the baby bug big time (as if we didn't already).
- Jordan celebrates his golden birthday - 28 on the 28th!
- I start volunteering at church with what is now called Realtime Crossroads. This turns out to be a much bigger commitment than I anticipated, but I LOVE it!!
- I get very sick... I swear it was the H1N1... and it lasts for 2 months. Not much to speak of in February except that I stayed in bed a lot.
- Then Jordan gets sick too, and it is tons of fun.
- We run tests on Jordan and I to check for possible fertility issues... all looks good with him, so we know the issue is me. One step in the right direction. Clomid it is.
- I need a distraction and get into sewing. I get a new sewing machine and become a sewing maniac.
- Jordan's band, Those Eighty Acres, are live on the radio for the first time - 88.1!
- I start my first round of clomid and it WORKS... except for the fact that we do not get pregnant on it. I am still hopeful though, as I know it can take 3+ months to truly work.
- The Access of West Michigan First Annual Gala happens!! It is a huge success and I continually feel blessed to work for such a great organization.
{the girls at Access}
- Clomid round two begins.
- The month to remember... we find out at the beginning of May that we are indeed pregnant... only to find out that we are miscarrying about 3 weeks later. I am totally devastated... totally and completely. {but of course God is good... this journey has forever changed me} You can read more about it here.
- Realtime Crossroads launches at church and we are now in full swing!
- Jordan and I take a much needed get-away up north and spend a lot of time reflecting, enoying each others company, playing scrabble, and going to Mackinaw Island.
- Jordan's band takes a mini-tour in Philidelphia, PA!
- On July 17 the unthinkable happens... we are pregnant again. Without drugs... completely naturally. I am thrilled and terrified at the same time. More here.
- My good friend Erin gives birth to John David! Yay more babies!
- I am frustrated with my OB... I was experiencing issues that I felt he wasn't paying attention to (especially given my recent miscarriage). So, I switch doctors... and on August 5 I see my babes heartbeat for the first time. Oh so amazing... and a true relief.
- I feel pregnant... sickness, food aversions, the whole she-bang. And I am thrilled about it. Although the rest of the summer is spent sleeping and puking.
- We buy a mini-van!! (this is significant on the road to parenthood)
- My good friend Jess gives birth to Leah Grace! Yippee!
- My friend, Mandy, tells me that she is pregnant again and due just 3 weeks before us! Double Yippee!!
- We reach second trimester... another GREAT sigh of relief!!
- We get a surprise ultrasound and are amazed at God's craftmanship.
- I turn 26! Happy Birthday to meee!
- The Access of West Michigan County Wide Food Drive collects over 87 tons of food! Way to go Grand Rapids!
- We go to Indiana for a family race! Ben did the marathon, and the rest of us did the 5K (Mom and I walked). Tons of fun!!
- I feel the babe move for the first time between 15 and 16 weeks. :-) So special!!
- A wonderful Thanksgiving with the Griffis/DeLooff family!
- Jordan is able to feel the baby move for the first time at 19.5 weeks.
- My good friend Kaylee gives birth to Bella Grace!
- Jordan and I take a little "baby-moon" to Chicago and see Wicked! Tons of fun!
- We are down to less than 100 days before we meet the little guy.
- We have an amazing Christmas week with my family. We are so blessed!!
- And now we are looking forward to an amazing 2011... meeting our little boy and watching our lives completely change.
Happy New Year!!


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Excuses? Unfortunately, with an 11-speed XTR M9000 group hanging off of the World Championship-winning Alma OMR frame, you won't have any, especially when your compatriots see the Fox Float Factory fork and Mavic Crossmax SL hoops adorning your bike. Alma is more than numbers, Alma is Soul. Alma has a racing pedigree, equal parts passion and special sauce that's been the process since 2006. It's a soul that anyone can experience pedaling it in the mountains it calls home. Are you ready for the Alma M-Team to reveal the weakest link - you?
There are currently no reviews for this product.
Frame Orbea Monocoque Race Carbon. Patented 4x4 evo construction. DCR Internal cable routing. XC racing geometry. Tapered Head tube. PF92 Press fit. Direct post mount disc brake. Di2 Ready.
Fork Fox 32 Float Factory 100 FIT4 3-Pos Remote Adjust QR15 Kashima
Crankset Shimano XTR M9000 26x36t
Headset FSA 1-1/8 - 1-1/2" Integrated
Handlebar FSA K-Force Flat 700mm Di2 ready
Stem FSA OS-99 CSI
Shifters Shimano XTR M9000
Brakes Shimano XTR Race M9000 Hydraulic Disc
Rear derailleur Shimano XTR GS Shadow Plus
Front derailleur Shimano XTR M9025 34,9mm
Chain Shimano HG900
Cassette Shimano XTR M9000 11-40t 11-Speed
Wheels Mavic Crossmax SL Pro TL 6-Bolt
Tyres Maxxis Ikon 2.20" TLR 120 TPI 3C Exo
Pedals N/A
Seatpost FSA K-Force Light SB0 27.2x400mm
Saddle Selle Italia SLR XC Gel Flow


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Yellow tea!
The last in the H&S Green Tea Sampler.
Steeping for the time and temp in the Harney note, approximately. The Breville doesn't have settings in 15 second increments, so I'm rounding up.
Beautiful, long fuzzy leaves/buds. They feel soft to the touch and remind me of little caterpillars. They have an interesting, nutty fragrance. It's sort of reminiscent of some oolongs and some darjeelings, but more delicate.
It's funny, I don't know that I would have come up with "spice scented rose", but wow, it's there in the aroma. Which is pretty interesting, since the aroma is very very delicate. It's the kind of thing where if you don't spend time with it, you could miss it.
Very very pale yellow liquor. Almost clear. The flavor has a spicy lilt to it, which I wasn't expecting at all. It is like a ginger, like a ginger that almost isn't there and makes itself known mostly through feel and secondarily through taste. I'm getting some nuttiness on the back end. Very mild nuttiness, like the meat of macademia nuts minus the salt.
This wasn't love at first sight for me, as I was sort of hoping it would be. But it's positive enough that I'm willing to go out on a few more dates and see what comes of it.