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Tea Party America doesn't look much like the base of the big-business Republican Party.
Last night, after weeks of the most literal government inaction, the Senate stepped in and brokered a deal to reopen the U.S. government and raise the debt ceiling. But despite the last-minute deal, the deeper fissures that produced the Republican standoff and the government shutdown are not going away any time soon. They reflect fundamental divides in America's economic and political landscape that have only deepened over time.
Over the past few weeks, politicians and commentators – from President Obama to a whole host of news outlets – have referred to the shutdown as the work of a few Republicans holding Congress and the economy "hostage." One particularly striking map (immediately below) by Ryan Lizza of the New Yorker has circulated around the internet for the last few weeks. It charts the districts of the 79 members of Congress credited as the instigators of the shutdown crisis, after they signed a letter to House Speaker John Boehner this summer demanding action on "de-funding" the Affordable Care Act.
The geography of Tea Party conservatives is largely what you'd expect. Half are in the South, and a quarter are in the Midwest. Not a single one is in the Northeast or the along the Pacific coast. All voted for Romney over Obama. As Lizza points out, using data from Cook Political Report's David Wasserman, the average district in this "suicide caucus" was three-quarters white, compared to 63 percent white in the average House district.
To underscore how different the Tea Party base is, my colleague Charlotta Mellander at the Martin Prosperity Institute ran a simple correlation analysis looking at the share of a state's congressional delegation that had signed the August letter to Boehner (and thereby made it into Lizza's "suicide caucus") and key economic and demographic characteristics of the state. As usual, we point out that this is only a preliminary analysis, and that correlation does not equal causation. But a number of interesting patterns appear, in light of both the maps in this post and what others have written about these diehard anti-Obamacare Republicans in the past few weeks.
First off, Mellander found states with higher shares of suicide caucus districts to be less advantaged, less affluent, and less educated. The percentage of suicide caucus districts was negatively correlated with wages (-.30), incomes (-.33) and college graduates (-.36). States with higher shares of suicide-caucus districts were also less diverse – both in terms of immigrant and gay and lesbian shares of the populations (with correlations of -.33 and -.32 respectively). States with more suicide caucus district are also markedly less urban. The correlation between the share of members in the suicide caucus and levels of urbanization was significantly negative (-.5). Mellander also found a positive correlation between the share of uninsured residents and proportion of suicide cause districts, a fact that National Journal's Ron Brownstein has noted.
Next, compare Lizza's map to the ones that Brownstein put together before last fall's election. These maps (below) chart the creative class vote (including scientists and technologists, business and management professionals, and arts, design, entertainment and media workers) by county in the 2008 Obama-McCain contest. (Keep in mind these maps use older data, gathered before the Tea Party even became a major force in American politics and they chart counties, not congressional districts).
Still, the basic pattern is clear. Notice how much of eastern Tennessee – a block of Tea Party dark red on Lizza's map – shows up in the bottom third of counties for creative class membership. Tea Party areas like those in southern Missouri and northern Indiana also show up in pockets of red on the bottom map of low creative-class density. And some of the darker red regions that appear on the first map below, representing the nearly 43 percent of high creative-class counties that went for McCain, tend not to overlap with the 79 members of Lizza's "suicide caucus" – through Nebraska and the Dakotas especially.
Now compare Lizza's map to the next map (below), which shows the share of Obama and Romney votes by metro area. The deep red metros are largely in oil and gas-dependent metros of Texas, though places like Salt Lake City unsurprisingly make the list as well. Apart from these larger Energy Belt metros, comparing this map to Lizza's shows how the Tea Party caucus represents the less urban, less dense places – the metros (and in general places that don't even meet the definition of a metro) that are well below the densities required for innovation and real economic growth.
The last map gets at the deep economic fissures underlying Tea Party politics. It's a map of America's centers of innovation, which I wrote about on Cities last week (and which my Atlantic colleague James Fallows used to make a similar point earlier this week).
Comparing this map to Lizza's shows that the Tea Party gets it stronghold in places that have been left behind by the knowledge economy. As a reader wrote to Fallows, the lack of overlap in this map of patents and map of Tea Party strongholds is revealing: "A minority of Americans seems committed to a past that never quite existed and hopes for a future that seems rather unlikely."
• • • • •
Taken together, these maps illustrate the deep economic fault lines underlying America's dysfunctional politics. The Tea Party represents the lagging sectors of the economy, and, increasingly, the politics of those left behind by America's transition to a new, knowledge-oriented economic future. As Colin Woodard writes over at Washington Monthly, the Tea Party's strength is concentrated in the Deep South, Appalachia (stretching to West Texas), and a region he calls the Far West. He explains:
There are tea party supporters everywhere, but only in these three cultural regions have they managed to achieve real and lasting political success. This is because their platform — to slash taxes, labor protections, environmental regulations, social programs, and the reach and authority of the federal government — is in accord with the centuries-old cultural ethos of each of these regions, and anathema to those elsewhere.
Writing in Salon, New America Foundation's Michael Lind notes that the Tea Party is a new version of old southern elite, no longer content with their place in an increasingly fractured Republican coalition. The Tea Party, he writes, reflects "a coherent and rational strategy for maximizing the relative power of provincial white elites at a time when their numbers are in decline and history has turned against them."
The Tea Party's tirade is not some passing fad, but a political outgrowth of the winners and losers of America's new economic landscape.
Top Image: U.S. Senator Ted Cruz (R-TX) and Senator Mike Lee (R-UT) depart the Senate floor after their speeches before the night-time budget vote at the U.S. Capitol on Wednesday. REUTERS/Jonathan Ernst


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Riots in Zaatari refugee camp [2] in the north of Jordan have lead to the army being called in, reports from the camp suggest.
The move came just hours after aid workers were attacked, as refugees protested against poor living conditions, Sky News reported. The camp has faced heavy rain and strong winds since Sunday evening.
A state of emergency was declared due to the weather, with 50 tents reportedly underwater and several swept away. Refugees in the flooded areas are being move to the on-site school and container 'caravans'.
The camp, situated near Mafraq, is home to 60,000 Syrians and has seen frequent protests over living conditions. On January 2, refugees rioted when the Jordan Hashemite Charity Organiastion handed out blankets.
In the first six days of 2013, 9,000 people crossed from Syria in to Jordan [3] and were placed in the camp, with over 22,000 crossing since December 1. Agencies dealing with the refugees were forced to increase staff numbers to keep up with the influx.
Bad weather has lead to a decrease in the numbers crossing, last night 427 crossed, compared to 923 the previous night. Yesterday 157 people returned voluntarily to Syria [4].
Snow is forecast for the end of the week in Jordan and despite a process of "winterization", refugees still fear the onset of colder nights.
Bad weather has been affecting the Kingdom since Sunday, with heavy rain causing problems in the capital [5], Amman.
A state of emergency has been issued in the city by the municipal authority who are compiling a list of tunnels to be avoided due to flooding and have all their staff working on clearing drains.
Jordan maintains an open border policy, allowing Syrians to cross freely and seek refugee. It is thought that 290,000 are now in the Kingdom, with the vast majority of the refugees residing in the villages and towns along the border.
[1] http://www.syndigate.info
[2] http://en.wikipedia.org/wiki/Zaatari_refugee_camp
[3] http://www.albawaba.com/news/syria-refugees-jordan-461684
[4] http://www.albawaba.com/news/syria-refugees-jordan-442321
[5] http://www.albawaba.com/editorchoice/jordan-election-461930
[6] http://www.albawaba.com

subset: commoncrawl


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The American Academy of Pediatrics is sending a message to parents today. They're urging everyone to have their children immunized against influenza as soon as the vaccine is available. Flu season can last anywhere from October through March, but because the flu virus can strike at any time, and because of international travel, health care professionals are asking parents to be proactive about immunization.
Recommendations of the AAP this season is that all children starting at six months get the flu vaccine as soon as its available and, there are choices.
Of the vaccines available, one protects against three strains and the other protects against four, but the AAP doesn't favor one over the other despite the added protection
Some parents with kids older than two prefer the nasal mist spray because its less painful and threatening to the kids.
The nasal spray is for children that don't have respiratory conditions or congestion, are allergy free, and not sick. Latest research finds those with egg allergies can receive the inactivated vaccine. The AAP does recommend pediatricians consult an allergist before vaccinating children with previous history of severe reactions.
To avoid a shortage this year the CDC is pledging to produce between 135 and 139 million flu vaccine doses.


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Source: Greentech Media After a year of negotiations, Public Service Electric and Gas Company reached a $1.2 billion settlement with the New Jersey Board of Public Utilities for its Energy Strong proposal. The approval of $1.2 billion over five years is far…
grid security
PSE&G Wins Approval for Pared-Down $1.2B 'Energy Strong' Plan
Arizona Commission Opens Benefit/Cost docket for DG
The Arizona Corporation Commission (ACC) has opened a docket and issued a memo on January 27, requesting comments on the process and methodology for establishing the costs and benefits of distributed generation (DG), including net metering systems. A number of DG costs and benefits were identified in an Arizona Public Service Net Metering Cost Shift Solutions docket that concluded late last year.


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Lenovo and IBM
I seem to recall that Lenovo are licensees of the Think Pad brand, not owners.
I seem to recall they signed a ten year licensing deal with IBM. Am I wrong ?
If so, this suggests confidence that IBM will re-license the brand out to the Chinese, or, given the growth in the market that Think Pad is enjoying, would IBM pull it back in house and enjoy the fruits of their own designs.... ?


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To not let my 1 year old breastfeed despite her crying in hunger?(96 Posts)
She's not eaten ANY food today, tried to give her porridge, apple puree, strawberries, 'organic' crisps, sweet potato and lasagne so far. She hasn't had a single mouthful, I last let her breastfeed about an hour after she refused breakfast, and she had a big drink then at about 10.
This is a very regular occurrence but she usually breastfeeds on demand a lot.
She's taken a tiny bit of water but is pushing away snacks still, and clawing at my top for milk, pretty upset.
Should I just give in or should I wait out and hope she takes real food when she's hungry enough?
Maybe she's not quite ready for the variety of solids? My two dc developed their solids at massively different rates. While ds1 very quickly moved onto lots of different textures and loved it, ds2 was a much more reluctant eater and had to be weaned on pureed baby food, introducing only one flavour every few days (or even weeks) until he was used to it.
Interestingly ds2 eats more 'extreme' flavours (chutneys, spices, etc) than ds1 does now that they are older.
I feel for you, it's really exhausting when you feel that you have to be the 24 hour diner even though they aren't babies any more.
Both my DSs had a patch like this around their first birthday, (and they were both great eaters before too) but they both then got back to "normal" within a few weeks and dropped back down to a few feeds. DS1 then fed til 18m and DS2 still is now. Developmental thing maybe??
I didnt want to wean, so grit my teeth and got through it, but it was a bloody pita when they were attached all day and night, so you are allowed to be annoyed.
are you just annoyed for now, or do you want help weaning?
I'm sorry but I'm just gobsmacked at the 2.4 year old who feeds every 30-69 minutes! Have you not gone anywhere or done anything for the last two and a half years??
Maybe she's ill. Or teething.
Or, a small chance, that she's intolerant to some foods or has tongue tie or reflux so doesn't get on with solids.
I would feed her. When it comes to meals, sit her down while you eat and have some bits of food which are simple - plain strips of meat, plain veg, plain pasta etc so she can try from your plate.
I was a bit surprised by that too Rhonda.
The 2.4 year old had multiple allergies - that's not surprising they BF so much.
My 24 month old feeds frequently still. Sometimes for comfort. Sometimes for food.
If he is refusing food it is for a reason and he will breastfeeders more. I let him, because it refuse him feels utterly wrong.
But, I am shattered. And touched out. It's hard
Thank you for all the helpful replies, I'm more worried about her not eating than the breastfeeding, I would like to cut it down a little bit, but realistically I know she's going to need it for a fair while longer.
It's just how little she eats, I'm sure I must be doing something wrong, she eats less than some 4 month olds I know!
If she had a couple of mouthfuls, eg porridge, for breakfast, a couple of mouthfuls of fruit/snack and maybe 1 mouthful each of lunch and tea, I would class that as one of her best days. She regularly eats nothing, or spits everything out so it's near to nothing.
I've seen 2 doctors, at 9 months when she had refused everything for 3 months, and then at 11 months when she'd been having tiny amounts but still not much. Both said not to worry as long as she's getting milk.
I don't think she's picking up on me being tense, during meal times I'm pretty relaxed as it's been so long of her not eating I just expect it, but I'm just running out of ideas. I felt awful not letting her feed earlier, and even that didn't work.
If I hand her my food, she will hand it straight back like she thinks it's a game, if I try to spoon feed her she will close her mouth tight and swing her head from side to side, then try to grab the spoon and shake the food off, if I put food infront of her on the highchair she will drop it off the side piece by piece like she thinks its a game, and if she does eat anything she usually looks disgusted then doesn't want any more.
I'm going to get flamed for this but have you considered some form of night weaning and sleep training? The frequent might feeding could explain lack of appetite. And without proper rest she won't be receptive to anything.
Oh and another thing that can help - for a few months I basically fed DD like a cat - plate of snacky food out to graze at will but no set meals. So sliced apples, cubes of cheese, breadsticks etc. I let her breast feed as much as she liked daytime as it reduced night time.
It passed. It's a phase
DD now at 2.4 eats solids well at mealtimes, has self night weaned and normally only breast feeds about 4 times a day
Message withdrawn at poster's request.
Ah but your previous post sounds like you give her the food. She doesn't have control.
Don't feed her and just put stuff on her tray. Eat at the table when she does and make sure it's in grabbing distance. occasionally get up from the table or look away or something, don't watch her.
Also offer snacks instead of a feed. Or better yet you have a snack (just reduce your meals at other times) and let her grab some.
Basically adopting a breezy attitude - because I'm wondering if she feels under pressure eg in a Highchair with you watching her.
If you're anxious about whether she is eating or not, she will pick up on this and be less likely to eat - it's a control thing.
I know that just saying "don't be anxious" isn't helpful - but you could try doing something else while she eats, or try to change your mindset. The book I recommended earlier could help with this. This is a great information sheet from LLL as well, your local group may have them to give out or for a nominal 10p donation.
Another thing I read recently is about how it's your responsibility as the parent to provide enough food, tasty food, healthy food, but it's their responsibility as a person to choose to eat it or not, and really and honestly they won't starve themselves. There is no point feeling anxious about something you can't control, so try to let go, keep doing the thing you do have control over (to provide the food) and don't worry whether she eats it or not.
Another thing you might find helpful is this:
In the second year (12-23 months), 448 mL of breastmilk provides:
29% of energy requirements
43% of protein requirements
36% of calcium requirements
75% of vitamin A requirements
76% of folate requirements
94% of vitamin B12 requirements
60% of vitamin C requirements
– Dewey 2001
(Full article: kellymom.com/ages/older-infant/ebf-benefits/)
I agree it would be worth checking her mouth BTW, but DS was exactly like this and didn't have anything wrong, he was just reacting to a stressful situation. Is there anything big going on for your family currently?
DD breastfed like mad until 12 months old, then switched to bottles of cows milk. Around 16 mo she was still having about 5 bottles per day and very little solids, then all of a sudden she didn't want it anymore and started eating everything in sight. Now she has the occasional cup of milk but really isn't fussed by it. So just presenting the alternative viewpoint that sometimes they will just get there in their own time
Ds went through a phase of refusing anything that I tried to give to him but put a bowl of scrambled eggs and a spoon in front of him and he would go at it.
I know you said it just gets dropped over the sides but if you give her a few tries without stressing, just clear up the mess, she may just sneak the odd bit into her mouth.
I'd feed her. Breast milk is full of good stuff. Can you feed her lying down with a book/ your phone so it's a rest for you? I basically used it as an excuse for a lie down when I was bf
I don't think yabu at all, at one my dd was eating everything and anything. I can totally understand why you are worried, does she eat with you at the table? Dd liked to be sat at the table with us and her own food to eat in front of her.
I would at this age bf max 4 times a day and stop feeding at night. You will prob need to do some cc in terms that she will have to learn another way to settle herself but in the long term this will improve her appetite during the day and obv help her and you in that she will be getting a decent nights kip.
I would also go back to the dr, maybe keep a record of what she eats for a few days to show them.
What would freak me out about this is what would happen if something happened to you? If you were ill or had to go away for a couple of days what would she do?!
please do not panic things change with DC very quickly its us who sees a behaviour then thinks its going to be like that forever, we also had food issues with DD1, she loooked so skinny compared to other babies, we saw a pead and everything. She was and is fine.
Relax, I worried more with first as she was not BF, so I was really worried about getting fresh food into her.
with no2, she is BF, on demand and just under a year we are taking it easy, offer her food off spoon, also little sarnies she can eat herself, a mixture, I do not stress if she hasn't had much.
I am looking to wean her off breast, but not yet, but very soon.
Your lucky you are BF she is getting as people have said - lots of amazing things from it. Yes babies start to need more at one, but not immediately at one.
Also try distraction, tv, in park....
By the way, my PEAD told me he knew of one child who would eat nothing but satsumas for a few years! said the child was fine and healthy!¬
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text:
Winter magic Achensee
Those of you who want to leave your skis at home during a winter holiday, will find numerous alternatives to the classic skiing holiday in Achensee and get to experience the snow-covered mountain landscape away from the ski pistes in a gentle manner.
Winter hiking
With every step the new snow crunches under your feet and thousands of ice crystals glisten in the cold winter air. There is hardly any nicer way to experience the magic of winter in Achensee than while winter hiking. The enchanting landscape around the lake is enticing, with more than 150 kilometres of the loveliest winter hiking paths which invite you to discover the natural beauty of Tirol 'step by step' and in complete tranquillity.
To the winter hiking routes in the region
Snow-shoe hiking
Away from the winter hiking trails there is the opportunity to discover completely pristine winter landscapes on snow shoes. Those who want to learn more about the natural surroundings and be safe out on the terrain can be accompanied by the region's trained mountain and hiking guides or participate in the original trapper tour into the Karwendel valleys from Pertisau.
To the snowshoe tour providers
To snowshoe hire
To the snowshoe tours in the region
Ice-skating and Curling
Whether Achenkirch, Wiesing or Pertisau – in the villages in the holiday region the ice rinks are open throughout winter. Here children and adults have fun while ice-skating and curling.
To the ice-rinks
To the curling rinks


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Just in time for Memorial Day, President Barack Obama has signed a law restoring paid leave time to Minnesota National Guard soldiers.
Getting a bill to the president was a bipartisan accomplishment for Minnesota's congressional delegation.
In the middle of a deployment to Kuwait and Afghanistan last year, members of Minnesota's Red Bull Brigade were surprised to learn that the Pentagon had changed the formula for how much leave the soldiers earned, effective immediately. Some of the soldiers were set to lose as much as 27 days of leave.
While the policy change affected nearly 50,000 Guard troops nationwide, it was a pair of Minnesota lawmakers who took the lead to alter it.
Led by Republican Rep. John Kline in the House and Democrat Sen. Amy Klobuchar in the Senate, they succeeded with overwhelming support in both chambers.
The bill that's now been signed into law means that any National Guard soldier deployed under the old leave policy will get to keep the time off he or she earned.


text:
Health workers and hospitals in war zones around the world are being actively targeted for attack, says the International Committee of the Red Cross (ICRC) which is launching a four-year global campaign to raise awareness of the issue.
"In Sri Lanka and Somalia, hospitals have been shelled; in Libya and Lebanon, ambulances have been shot at; in Bahrain, medical personnel who treated protesters are on trial; and in Afghanistan, the wounded languish for hours in vehicles held up in checkpoint queues," the ICRC says in its report 'Health care in danger: making the case'.
The attacks are not limited to particular conflict zones, countries or continents but are widespread, deliberate and on the increase.
"From Colombia to Gaza, and from the Democratic Republic of the Congo to Nepal, there is a lack of respect for the neutrality of health-care facilities and personnel, and medical vehicles, among both those attacking them and those who misuse them for military gain," says the ICRC.
The effects are reductions in people willing to undertake the increasingly dangerous, life-saving work in warzones as well as knock-on effects that reach into war-affected communities where vaccination programmes grind to a halt or routine healthcare cannot be given.
The ICRC is calling for an end to the "common" practice of targeting health workers and hospitals in war zones.


text:
Train St Brieuc → RennesFrom HK$0
Find a Train Ticket
Overview The average travel time between St Brieuc and Rennes is 1h39 minutes. The quickest route is 44 minutes. The first train leaving St Brieuc is at 05:00, the last at 22:25. There is an average of 38 trains a day between St Brieuc and Rennes, leaving approximately every 13 minutes.
- Arrival station : Rennes (19, Place de la gare BP 90527 35005 Rennes cedex)
Why choose us?
5.5 milliontravellers in 2015
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Main stations in St Brieuc and Rennes
Arrival station
Rennes Train Station
Address: 19, Place de la gare BP 90527 35005 Rennes cedexOpening hours: Monday to Friday: 5am to 12:45am
Saturday: 5am to 12:45am
Sunday: 6am to 12:45am
Trains operating in and from this station:
High speed trains (TGV), Regional trains (TER), Intercity trains
SNCF rail agents, Assistance for disabled persons, Business lounges & Waiting rooms, Disabled facilities, Currency Exchange, Lost and found, Tourist Information Point, Toilets, Luggage storage, Wi-Fi Internet, ATM and Phone cabins.
Travel inspiration in St Brieuc and Rennes
St Brieuc
Reviews & ratings St Brieuc - Rennes
"Trains in France" Catherine m. 09/10/2015
As usual train on time and good prices if you book well in advance
As usual train on time and good prices if you book well in advance


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Bi-amping and tri-amping
This article needs additional citations for verification. (August 2007) (Learn how and when to remove this template message)
Bi-amping and Tri-amping is the practice of using two or three audio amplifier channels to power a loudspeaker with multiple drivers.
"Bi-amping" is the use of two channels of amplification to power each loudspeaker within an audio system. "Tri-amping" is the practice of connecting three channels of amplification to a loudspeaker unit: one to power the bass driver (woofer), one to power the mid-range and the third to power the treble driver (tweeter). The terms derive from the prefix bi- meaning 'two', tri- meaning 'three', and amp the abbreviation for "amplifier".
It differs from the conventional arrangement in which each channel of amplification powers a complete speaker. Bi-amping typically consists of a crossover network and two or more drivers. With ordinary loudspeakers, a single amplifier can power the woofer, mid-range and tweeter through an audio crossover, which filters the signal into high- medium- and low-frequencies (or high- and low-frequencies in 2-way speakers) – a mechanism that protects each driver from signals outside its frequency range. However, the passive crossover itself is inefficient, so splitting the frequencies electronically before these are amplified is a way to avoid this problem. In such a case, an amplifier each powers a frequency range determined by an active crossover to each of the drive units. The technique is primarily used in large-scale audio applications such as sound amplification for concerts, in portable powered speakers and by hi-fi enthusiasts.
A speaker system has to be wired to accommodate either configuration, typically with two sets of binding posts, one set for the bass and one set for the mid-highs. A single amplifier can usually power a woofer and a tweeter only through a crossover filter, which protects each driver from signals outside its frequency range.
Bi-amping of speakers requires double the channels of amplification and can be accomplished using two ordinary amplifiers in either a vertical or horizontal arrangement.
- Horizontal bi-amping uses one amplifier to power both bass drivers (woofers) and the second amplifier to power both treble drivers (tweeter) or the midrange and treble drivers together. Horizontal bi-amping has the advantage of allowing two different amplifiers that sound better than each other for bass or for treble.
- Vertical bi-amping uses two channels of an amplifier per loudspeaker, with a dedicated channel for the bass driver and a dedicated channel for the treble or the treble and the midrange together. Vertical bi-amping has the advantage of not having to use a single amp to power both bass sections, which can be very taxing on the amplifier, especially at higher volume or if the bass driver has a particularly low impedance at certain frequencies.
Most audible differences are subtle. If at all noticeable, benefits of bi-amping cannot be realized if passive crossover networks of a speaker system are not removed.[1] Benefits include transients are less likely to cause amplifier overload (clipping) and/or speaker damage, and reduced intermodulation distortion, elimination of errors introduced by low frequency passive crossover, reduction of load to the load presented to the power amplifier, better matching of power amplifier and speaker driver and others.[2]
See also[edit]
- ^ An article from Audioholics online A/V magazine, 30 August 2004 , The Difference Between Biamping vs. Biwiring
- ^ Article by Elliot Sound Products, Benefits of Bi-amping, not quite magic but close


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ts bill looks pretty promising for our region late next week...what you thinkin the chances wel get some ill nuggie waves outa this system swellinfo
Results 1 to 10 of 23
Thread: Trop storm Bill
Aug 16, 2009, 03:10 PM #1Junior Member
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- Jan 2009
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Trop storm Bill
Forecasts are shady at best b/c of path predictions 5 days in advance are shaky...I'd check back in three or four days. Golfing I hear is a good sport
golfing isn't a good sport it is a great sport... my mantra is surf & turf... what is ill nuggie??? 8ft+ or mung infested closouts... seriously did you really just type that... one wave at a time my man, one wave at a time
Aug 17, 2009, 01:41 AM #4
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i woodn't call that a 'weak' path'. yea, it IZ veerin' outta sea, but at that strength and position, no doubt we'll c atleest 2dayz uv solid surf, and s**t, that's more than 1 cood ask 4 around this s**thole...
Aug 18, 2009, 02:47 AM #6
its not shady its going to hopefully produce some sorta surf able swell better then what we have been getting I'll tell you that much.
that l8est projection'z puttin' Bill pretteey F&*ckin' closeta wrong island!?
Aug 18, 2009, 01:06 PM #8Junior Member
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- Jan 2009
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triple OH sundayy?!!? .. could any places on LI hold a solid 12 ft long per swell like that
I've never even seen the maps hold that much swell, let alone a beach.
Aug 18, 2009, 05:32 PM #10


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" Brand: Tesco / Type: Other Ready Meals "
My mum brought these ready prepared sprouts from Tesco to go with Xmas dinner, she never goes to the stress of buying and peeling her own because she hates them with a passion and only buys them for my aunt and cousin! lol
These ones come in their own pot and can be microwaved in a few minutes so that makes them ideal when you're cooking a massive meal like Xmas dinner. They don't smell as putrid as boiling sprouts and I was happy to go into the kitchen while these are cooking!
When they were done I told my mum that I'd have a couple on my plate to try because the smell of bacon was lovely and strong on them. I'm not a fan of brussels sprouts but these weren't THAT bad, they were deffo cooked better than the sloppy balls my mum usually dishes out and had a slightly crunchy texture in the centre of each sprout.
The bacon and shallots are just cubes of hot ham and onions, they have been added to the sprouts but had all sunk to the bottom of our bowl of sprouts so I reckon you should give it all a good shake before cooking and a good stir afterwards!
I wasn't mad keen on the texture of the bacon cubes because some of them was proper fatty, they don't taste as strong as they smell but they have given the sprouts a bit of a smokey flavour. The onions just season the sprouts I reckon because they're not strong enough in taste to overwelm the sprouts but they give the whole thing a nice onion aftertaste.
We paid about £1.79 for this bowl of sprouts and that's not a bad price, I know sprouts are cheap anyway but at least with these they're all good to go and they are going to turn out loads nicer than a plate of squishy green mush on your plate!


text: I'm looking into adding a Kenne Bell to my '97 Mustang. The motor is a DSS long block stroked to 5.0 with Twist Wedge heads and Anderson Motorsports cams. So I was trying to decided which would be the best blower (from KB) for this application and noticed that the larger Mammoth models aren't sold for 96-98 2V. I called KB tech line and asked them which unit would work for my car and they said they don't make a model for 96-98 anymore, but people have installed the model for 99-04 with some modifications. This is where I'm looking for help. He hashed over some of the mods that would need to be done, but I'm not sure all of them will be necessary. First of all, the block is not a '97. I don't know exactly what year it is, but I'm trying to find out. My point is that the only thing that is really from a '97 GT on that car is the ignition, wiring harnesses, alternator, brackets and such. He said that the block would have to be ground down somewhere around the "lifter valley," alternator bracket would need to be changed, ignition would need to be swapped to coil-on-plug system, as well as some other mods for sensors and such. So, if anyone has done this kind of install or know of any other threads or sites about it, I'd appreciate your help.


text:
Get to 5760x1200 and we can talk #loveFFX#
Unreal 3 Engine has been the downfall of many games...about 50 percent of all games who use UE3 looks just plain UGLY...I suggest you guys stick to NanoFX.
That was because they were not aware or had the full SDK to work with. The UT3 engine is fantastic, but it has it's limitations. The Evolved engine is far more modular than the UT3 engine, so you can say that the Evolved engine should age extremely well over the next few decades. :wink:
My brother is actually working on that! I hate to say this, but I already seen this about a month ago. Very cool stuff to be sure. DX11 is needed for all of the effects planned for the U3 engine.
The Effects seen in the Video are all DirectX9 tech. Even the High Quallaty DoF is only rendered using the DirectX9 Tech Level. The Lightshafts are simple and inaccurate but very good looking approximations using a Screenspace effect, therefore there is no magic there. The colorgrading by Michael Mittring is fast and effective and has already been used in the Crysis DX9 renderer.
Cry-engine 3 works on XB360 though, and that's DX9c isn't it?


text: DSTGames 124 Report post Posted January 18, 2011 (edited) Do you need music for your game? Are you using copyrighted music, or bad music, or no music at all? Well, I am giving away 100's of tracks of game music to indie game devs for free! This music is all composed by me and all samples/instruments in them are licensed in full, so this music is completely royalty free. You can find the collection at : [url="http://www.nosoapradio.us"]http://www.nosoapradio.us[/url] The tracks are sorted by Genre. Thanks, and enjoy! Edited September 10, 2015 by Gaiiden 2 Share this post Link to post Share on other sites


text:
Despite killing off showrunners almost as quickly as it does castmembers, AMC's The Walking Dead continues lurching to record ratings. Sunday's Season 3 finale drew 12.4 million viewers, edging History Channel's Easter Sunday conclusion of The Bible. The miniseries ended with Jesus' resurrection, pulling in 11.7 million viewers. History has learned the lesson. Documentaries about history equal low ratings. Scripted dramas that take viewers inside the history books can garner record ratings. Fresh off the success of Hatfield & McCoys, the network appears to have a new ratings blueprint.
Speaking of history, the Big Four networks were looking pretty outdated on Sunday. The Walking Dead and The Bible finale clobbered them. Not only that, AMC's recap show Talking Dead got more than five million viewers. That's right, a panel discussion about a hit cable show did a better rating than any show on network TV.
The Walking Dead's TV adaptation has to be different from the comic, since millions have already read the book. The season finale was its biggest departure yet from the source material, as Andrea dies from wounds she sustained battling an undead Milton. Her character is one of the book's longest living survivors. In one interview, Dallas Roberts, who played Milton Mamet, joked that actress Laurie Holden was one of the few not worried about being killed off. He has revealed that they shot Andrea's death scene months ago. He knew while he was giving interviews about his character's fate. Don't feel too bad for Roberts. He has landed a gig on CBS drama Unforgettable. Not to mention, he doesn't have to play coy with his interviews anymore.
Andrea's TV incarnation was never as popular as her comic book counterpart. She developed a penchant for being attracted to the eventual villain the last two seasons. First she and Shane got together. That made her oblivious not only to how evil he was, but that he was still in love with Lori.
As bad as Shane was, The Governor made him look stable and well adjusted. Most women would have explained that it's not you, it's me after finding out he kept a room full of zombie heads. Andrea passed on multiple opportunities to kill him. Before she had to kill herself, she explained that she "just didn't want anyone to die." Andrea's death reinforces that on the TV version of The Walking Dead, no one is safe. Although I still like Rick Grimes' chances to survive all the way until the end.
Speaking of Rick, he's really got a problem child on his hands. It looked like we were going to see a simple redemption storyline for Carl. Last season, he was less popular than Andrea. But he stepped it up in a big way. In embracing Michonne as part of the group, Carl appeared to be maturing. Then he gunned down a boy not much older than him, who was obviously going to surrender. When Rick called him on it, Carl responded with some pretty good reasoning.
Almost every stranger the group has shown any mercy to has tried to kill them. While watching the scene, I wasn't sure the guy was going to surrender. The actor played it perfectly, you could see the fear in his eyes. But in the world of The Walking Dead, it's almost impossible to trust a stranger.


text:
My debt collection company agreed to accept three hundred dollars a month for 3 months as a payment plan. I agreed to allow them to take the money via electronic withdrawal on 3-29-06 and they did. They sent me a letter asking to withdraw another $300 on 4-30-06. I did not agree and called them to let them know there ...Read More »
Bank Accounts
What can I do Now? Some years ago, My ID was stolen
Some years ago, My ID was stolen, Subsequently, they acquired a credit card in my name. I didn't find out about any of this until I went to refine my home several years later. When I was told I had a default judgment for $11, 283.44 against me for the card. I called the company but all they wanted to ...Read More »
What about unauthorized Electronic Funds Transfers (EFT)?
Answer: The Electronic Fund Transfer Act protects you against unauthorized electronic transfers and may entitle you to monetary damages and free attorney representation. You can receive up to $1,000 statutory damages plus any actual losses if your EFTA rights have been violated. Learn more. Additionally, if you do not report an unauthorized transfer that appears on your statement within 60 ...Read More »
Can collectors withdraw funds from my bank account without my approval?
Answer: Although unethical collectors (yes, there are ethical collectors out there) threaten to take your money, doing so without your permission requires a court order! However, it happens more often than people realize. When it does happen, you're dealing with an unethical collector who uses a variety of methods to steal your money. Some of their favorite methods include getting ...Read More »


text:
The UFC will look to keep the momentum rolling from a stellar Kansas City card when they head to Nashville on Saturday for the first time since 2015.
If you're hoping to turn the event into an opportunity to build your DFS bankroll, DraftKings.com has you covered with a full slate of contests. Players get a $50,000 budget to select five fighters, and the scoring is distributed as follows:
(Please note that DraftKings has altered their scoring system. The new point values are listed below and the rosters will now expand from 5 to 6 fighters).
Moves Scoring
Significant Strikes (SS): +0.5 PTS
Advance (ADVC): +3 PT
Takedown (TD): +5 PTS
Reversal/Sweep (REV): +5 PTS
Knockdown (KD): +10 PTS
Fight Conclusion Bonuses
1st Round Win (1rW+): +90 PTS
2nd Round Win (2rW+): +70 PTS
3rd Round Win (3rW+): +45 PTS
4th Round Win (4rW+): +40 PTS
5th Round Win (5rW+): +40 PTS
Decision Win (WBD+): +30 PTS
Scoring Notes
Significant Strikes are any Distance Strike or Clinch/Ground Strikes that are considered "Power Strikes" by official scorers.
Advances include: To Half Guard, To Side Control, To Mount, To Back Control
Now, on to the fights...


text: I know a few lads on here run them. Just to see what needs to be done to do this. As i have been told they cause problems, but i would doubt you would still ahve them on your car if they did. I have a non-DBW 1.8t '97, was looking at getting one as there ain't no better noise than hearing a car DUMP. Looking for some heads up on what needs to be done or modified to stop errors. And who supplys them Or if it can even be put on a car as old as mine. /ubbthreads/images/graemlins/tongue.gif


text:
this tea tastes like incredibly juicy chocolate covered strawberries. There is a delicious black tea taste underneath though that makes this amazing all the way down. I generally can't handle rose petals in tea but this one I don't even taste them. I suspect they might come out more when it's iced. A great late morning, early afternoon pick me up!
4 min, 30 sec


text:
MULTNOMAH, Wash., Aug. 9 (UPI) -- A Washington state outbreak of E. coli in strawberries has caused at least one death and made at least 16 people sick, public health officials say.
Oregon Public Health officials linked the outbreak to Jaquith Strawberry Farm in rural Washington County, The (Portland) Oregonian reported.
The medium-sized farm had sold strawberries to buyers who then distributed them to roadside stands and farmers markets in Multnomah, Washington, Clackamas, Yamhill and Clatsop counties. Farm production of the potentially tainted strawberries was halted July 29 and the last of the berries thought to be contaminated with E. coli were sold on August 1.
Of the four people who have been hospitalized due to E. coli, one has died of kidney failure.
The last illness from the bacteria was reported July 29, but as E. coli has a 10 day incubation period before symptoms surface, health officials are expecting a few more cases to be reported.
"We're likely to see a few more cases trickle in for a bit, but the stuff is gone from the shelves," Dr. Paul Cieslak of Oregon Public Health said. "If you haven't become sick by 10 days, you're not going to."
William Keene, senior epidemiologist with Oregon Public Health, says deer he saw roaming through the fields may have been the source of the outbreak.
"The guy was unlucky enough to have an animal with the wrong bacteria wandering through his fields," Cieslak said.


text:
I need an american flag to use it as a link on my homepage of my website (www.eduardlid.net)! what´s the best place to get it?
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Brazilian Journal of Plant Physiology
On-line version ISSN 1677-9452
Braz. J. Plant Physiol. vol.14 no.3 Londrina Sept./Dec. 2002
Evidence of an endogenous lectin receptor in seeds of the legume Cratylia floribunda
Evidências para um receptor endógeno da lectina em sementes de Cratylia floribunda
Márcio Viana RamosI; Liezelotte Rezende BomfimI; Glaís de Paiva BandeiraI; Henri DebrayII
IDepartamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Campus do Pici, CP 6033, CEP 60.451-970, Fortaleza, CE, Brasil
IIUniversité des Sciences et Technologies de Lille , Laboratoire de Chimie Biologique et UMR n°. 8576 du CNRS, 59655 Villeneuve D'Ascq Cedex, France
Cratylia floribunda seeds were ground and the clean crude saline extract was fractionated into albumin, globulin, prolamin, acidic and basic glutelin protein fractions. These protein fractions were examined for the presence of an endogenous lectin receptor by SDS-polyacrylamide gel electrophoresis, western blot, affinity chromatography on a Sepharose 4B-Cratylia floribunda (CFL) lectin column and kinetic analysis in real time by surface plasmon resonance (SPR). Prolamin was the richest protein fraction although very poor in haemagglutinating activity. Basic glutelin was far the less interesting fraction for lectin activity and protein content, even though this fraction contains considerable amounts of carbohydrates. Lectin was present in all protein fractions as estimated by haemagglutinating assays but basic glutelins were almost devoid of lectin activity. Except for prolamins, protein bands were detected by SDS-PAGE in all other fractions. Western blot using digoxigenin labelled Con A revealed a single band in the albumin, globulin, acidic and basic glutelin fractions, which specifically interacted with ConA. This band migrated exactly at the same position in such fractions and seemed to be more important in the globulins. Affinity chromatography of the protein fractions on a Sepharose-CFL column yielded a peak, which was only recovered after elution with acidic buffered solution or with an a-D-mannose solution and the monosaccharide was recognized by the lectin. These results were fully corroborated by real time interaction of immobilized CFL with the different soluble protein fractions suggesting the presence of a lectin receptor within albumins, globulins and basic glutelins. As a whole, the results suggest that the lectin from Cratylia floribunda recognizes a soluble endogenous glycosylated receptor through an interaction mediated by its carbohydrate-binding site.
Key words: protein-carbohydrate interaction, surface plasmon resonance, Western blot.
Sementes de Cratylia floribunda foram moídas e o seu extrato salino solúvel foi fracionado para obter-se as frações protéicas albumina, globulina, prolamina, glutelina ácida e glutelina básica. Estas frações protéicas foram examinadas quanto a presença de algum receptor endógeno para a lectina da semente através de eletroforese em gel de poliacrilamida em presença de SDS, western blot, cromatografia de afinidade em coluna de Sepharose 4B contendo a lectina de Cratylia floribunda (CFL) imobilizada e análise cinética em tempo real baseada em ressonância plasmônica de superfície. A fração prolamina foi a mais rica em proteínas, embora pobre em atividade hemaglutinante. Glutelina básica foi a fração menos interessante quanto ao teor protéico e atividade hemaglutinante, embora tenha considerável teor de carboidratos. A lectina estava presente em todas as frações protéicas de acordo com os testes de atividade hemaglutinante, porém a fração de glutelinas básicas quase não apresentou atividade. Exceto as prolaminas, bandas protéicas foram detectadas em todas as outras frações protéicas por SDS-PAGE. A técnica de western blot usando Con-A conjugada a digoxigenina revelou uma única banda nas frações albumina, globulina, prolamina, glutelinas ácidas e básicas, que interagiu especificamente com a ConA. Esta banda apresentou exatamente a mesma localização nestas frações e pareceu mais evidente nas globulinas. Cromatografia de afinidade das frações protéicas sobre a coluna de Sepharose 4B-CFL produziu um pico que foi eluído da coluna apenas após a adição de tampão glicina ou solução de um açúcar inibidor da lectina (a-D-manose). Estes resultados foram completamente confirmados através dos estudos de interação em tempo real das mesmas frações com a lectina CFL imobilizada, também sugerindo a presença de um receptor endógeno nas frações albumina, globulina e glutelinas básicas. Como um todo, os resultados sugerem que a lectina de sementes de Cratylia floribunda reconhece um receptor específico e que esta interação deveria ser mediada pelo sítio de interação a carboidratos.
Palavras-chave: interação proteína-carboidrato, ressonância plasmônica de superfície, Western blot.
Lectins are carbohydrate-binding proteins widely distributed in nature (Van Damme et al., 1998; Rudiger et al., 2000). Their ability to specifically recognize and reversibly bind to soluble or cell bound complex carbohydrate structures make them special candidates to play different roles in many biological processes such as cell to cell interaction, ligand-receptor signalizing or cellular activation, among other important biological events (Wu et al., 1995; Gorocica et al., 1998; Sáez et al., 1999). However, the precise functions of plant lectins are still depending on more conclusive experimental evidence. Although many reports focusing on their role in nature have given important insights in this matter, endogenous lectin receptors in plants have not been fully described or characterized. Most of the obtained results suggest that plant lectins may take part in the plant defence system together with chitinase, glucosidase and protease inhibitors (Etzler, 1998). Legume lectins are also expected to participate in specific symbiosis involving roots and bacteria Rhizobia (Diaz et al., 1989; Naeem et al., 2001). Together, these hypotheses support the idea that the functions of plant lectins are addressed to foreign organisms and thus the hypothesis for the occurrence of endogenous receptors is currently unattractive (Peumans et al., 2000).
In the 1980s and early 1990s, a German group devoted some attention to the possible occurrence of endogenous lectin binding compounds following the idea that legume lectins which are vacuolar proteins stored in the protein bodies in seeds, could be the natural ligands for other glycocompounds located in the protein bodies, including their membranes (Einhoff et al., 1986; Rudiger and Schercher, 1993; Gers-Barlag et al., 1993; Schercher and Rudiger, 1994; Wenzel and Rudiger, 1995). Although their results did point to the interaction of the assayed lectins with some endogenous compounds, differences in the carbohydrate binding specificity of the lectins and evidence of a complex of lectins and stored proteins or membrane components mediated by ionic strength prevented more conclusive results. Furthermore, no endogenous ligand was isolated or characterized. Despite these results, it is of great importance to document the interaction of lectins with any endogenous compound, as it will certainly contribute to clarify the role of these proteins in plants.
Cratylia floribunda is a legume tree whose seeds possess a Con A-like lectin (Oliveira et al., 1991). ConA, the lectin isolated from Canavalia ensiformis seeds and Cratylia floribunda lectin, named CFL, can be isolated in a single step by affinity chromatography on a Sephadex G-50 matrix and share many biochemical and structural characteristics including their identical carbohydrate-binding specificity (Ramos et al., 1996a; Ramos et al., 1996b; Dam et al., 1998; Dam et al., 2000). In this report we present evidence for the presence of a Cratylia floribunda lectin (CFL) glycosylated receptor within the seed flour protein fractions, which may be recognized by the Cratylia floribunda lectin and by Con A, the lectin from Canavalia ensiformis seeds.
Material and methods
Lectin isolation and immobilisation: Cratylia floribunda seeds were collected in the state of Ceará, Brazil. The purified lectin was obtained by affinity chromatography on a Sephadex G-50 column as described by Oliveira et al. (1991). Material purity was checked by 12.5 % SDS-PAGE according to Laemmli (1970). The purified lectin (30 mg) was coupled to CNBr-activated Sepharose 4B (Pharmacia) (10 mL) according to the procedure described by March et al. (1974). After immobilization, the amount of soluble lectin still remaining in the supernatant (uncoupled) was estimated by spectroscopy at 280 nm as 4.02 mg. From the difference, the amount of bound lectin was considered to be close to 2.5 mg per mL of gel.
Preparation of protein fractions from the crude extract: Hulled seeds were ground and the fine flour was suspended in 500 mM NaCl (1:10, w/v) for two hours under shaking, followed by centrifugation at 20.000 gn at 4 oC for 20 min. The pellet was extracted again under the same conditions and the new supernatant joined to the previous one. This fraction, named crude extract, was dialysed against distilled water and centrifuged again under the same conditions. The supernatant, named albumins, was lyophilized. The precipitated fraction was dissolved in 500 mM NaCl, followed by dialyse against water and lyophilized. This fraction was named globulins. Prolamins were obtained by extraction with 70 % (v/v) ethanol of the pellet obtained after centrifugation of the crude extract. The new fraction was centrifuged as above and the supernatant submitted to dialyse and lyophilization. The new pellet was then extracted with 100 mM HCl and centrifuged. The supernatant was named acidic glutelins and was lyophilized after dialysis. Finally, the pellet resulting from this centrifugation was suspended and extracted in 100 mM NaOH and centrifuged. The new supernatant was dialysed and lyophilized and named basic glutelins. The pellet was discarded.
Haemagglutinating activity: The haemagglutinating activity of protein fractions was determined according to Ramos et al. (1996a,b) using 2 % (v/v) rabbit erythrocyte cell suspension in NaCl 150 mM. Analyses were done in duplicate and haemagglutinating potency was expressed as haemagglutinating units (HU).
Protein estimation: Protein in soluble fractions was estimated by the Bradford (1976) method using bovine serum albumin as standard.
Carbohydrate detection: The presence of carbohydrate in fractions was estimated according to Dubois et al. (1956) using glucose as a standard.
Gel electrophoresis: The purified CFL and protein fractions obtained by fractioning the crude saline extract of the Cratylia floribunda seed flour were examined by 15 % polyacrylamide gel electrophoresis under denaturing and reduction conditions in the presence of sodium dodecyl sulphate (SDS) and 2-mercaptoethanol according to Laemmli (1970). Samples of albumins, globulins, acidic and basic glutelins (2 mg.mL-1), prolamins (4 mg.mL-1) and lectin (1 mg.mL-1) were treated with tris buffer pH 6.8 containing 2 % SDS and 5 % b-mercaptoethanol at 100 oC for 4 min. and analysed. Run was performed at 40 mA at room temperature. Gels were stained with coomassie brilliant blue solution in water: acetic acid: methanol (8:1:3.5) and destained with the same solution without the dye or used for western blotting analysis.
Western blotting: Polyacrylamide gel electrophoresis of CFL and protein fractions prepared as above, was electrotransfered on a nitrocellulose membrane (Life Technologies, USA) at 40 mV overnight at 4 oC. The remaining gel was further stained by coomassie brilliant blue solution to confirm the transfer of all the protein bands onto the nitrocellulose membrane. The nitrocelullose membrane was revealed with 0.2 % ponceau S in 3 % trifluoroacetic acid (v/v) and destained with PBS. The nitrocellulose membrane was blocked with 2% polyvinylpyrrolidone for 1 h at room temperature followed by five washes with PBS. The membrane was then incubated with a solution of digoxigenin- labelled ConA (Calbiochem, USA) in PBS for 3 h at room temperature and further washed with PBS. Then incubation with rabbit anti-digoxigenin antibodies coupled to horseradish peroxidase (Calbiochem, USA) was performed for 1 hour followed by washing with PBS. The membrane was revealed by incubation with 15 mL of diaminobenzidine (Calbiochem, USA) in urea/H2O2 solution until the appearance of bands.
Affinity chromatography on a Cratylia floribunda lectin-Sepharose column: To perform the affinity chromatography of albumin, globulin, prolamin and acidic and basic glutelin fractions on the lectin column, the lyophilized fractions solubilized in 0.15 M NaCl (1 mg.mL-1) were applied to the column and eluted first in 150 mM NaCl and then with 50 mM glycine-HCl buffer pH 2.6 in 150 mM NaCl. Fractions of 1.5 mL were recovered using a flow rate of 10 mL.h-1. Absorbance of fractions was followed at 280 nm with a Ultrospec 1000 spectrophotometer (Pharmacia).
Kinetic interaction: Kinetic studies of soluble protein fractions with immobilized CFL were measured in real time based on surface plasmon resonance technology using a BiaCore 3000 instrument (Pharmacia Biosensor) (Zeng et al., 1998; Satoh and Matsumoto, 1999; Canziani et al., 1999). The purified CFL (200 mg.mL-1) was bound to the dextran layer of the CM-5 sensor chip using the amino coupling kit following the manufacturer´s instructions. For coupling, the lectin (1 mg.mL-1) was dissolved in 10 mM acetate buffer pH 4.0. Non-reacting remaining groups were blocked with ethanolamine. The protein fractions (1 mg.mL-1) (albumins, globulins, prolamins, acidic and basic glutelins) were injected on the cell surface of the sensor at a flow rate of 5 µl.min-1 for 300 s (association phase) and allowed to dissociate in equal time in HBS buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005 % (v/v) surfactant P20, BiaCore). Interaction intensity was considered as the difference between values of arbitrary resonance units (RU) at the moment of injection and the beginning and the end of the dissociation phase reducing the bulk contribution caused by passage of the sample under the cell surface. For inhibition assays, 100 mM a-methylmannoside was injected at the end of dissociation phase and allowed to interact for 300 sec, followed by the running buffer (HBS). All experiments were conducted at 25 °C. Flow cell regeneration was achieved by two successive injections of 10 mM HCl and 10 mM NaOH to remove the remaining material still bound to the sensor surface. To confirm performance of lectin immobilization, interaction with the glycoprotein soybean agglutinin, known to be a very potent ligand to CFL and inhibition of this interaction was performed under the same conditions.
Protein fractions obtained from the crude saline extract of the Cratylia floribunda seed flour were characterized for protein and carbohydrate content and analysed by polyacrylamide gel electrophoresis under dissociating conditions. These fractions were also examined for interaction with the purified seed lectin CFL by affinity chromatography on a Cratylia floribunda lectin-Sepharose column and kinetic analysis based in surface plasmon resonance technology (RPS).
Table 1 summarises the protein content of the albumin, globulin, prolamin, acidic and basic glutelin fractions. Prolamin was the richest protein fraction but very poor in haemagglutinating activity. Basic glutelin was the less interesting fraction for lectin activity and protein content, even though it contained a considerable amount of carbohydrates. Lectin was present in all protein fractions as estimated by haemagglutinating assays but basic glutelins were almost devoid of lectin activity. The presence of CFL in the fractions was further confirmed by SDS-PAGE (figure 1). The alpha chain and both beta and gamma fragments of CFL (Ramos et al., 1996a; Grangeiro et al., 1997) were present as the main protein band in the albumin fraction and also as an important component of globulins, acidic and basic glutelins. In spite of the amount of protein estimated by the Bradford (1976) method in the prolamin fraction, no protein bands were detected in this fraction by SDS-PAGE. This fraction was strongly coloured and protein content might be over estimated by the interference of organic compounds other than proteins. The same phenomenon seemed to occur when this fraction was examined at 280 nm after affinity chromatography.
Affinity chromatography profiles of the protein fractions on the Cratylia floribunda lectin-Sepharose column are shown in figure 2. After washing the column with the starting solution (150 mM NaCl), glycine-HCl buffer was used to elute possible lectin ligands from the column. Intensity of absorbances at 280 nm during washing of the column matched exactly with the protein content previously determined by the Bradford method (Table 1). It is noteworthy that the prolamin absorbance peak confirmed the estimation of protein content within the whole fraction. Nevertheless, it did not show any protein band stained by coomassie in SDS-PAGE (figure 1). Passage of glycine buffer through the column yielded changes in albumins, globulins and acidic glutelins absorbance, giving rise to a small peak while no peak was obtained from the prolamin and basic glutelin fractions (figure 2). The fractions recovered after the addition of glycine-HCl buffered solution were assayed for carbohydrate by the Dubois (1956) methodbut this method was not sensitive enough to detect carbohydrates in the fractions.
Western blotting analysis was a good strategy to search for lectin interacting material in the fractions. For this purpose, digoxigenin-labelled ConA was chosen. ConA-digoxigenin was used because it is commercially available and because ConA has very similar carbohydrate-binding specificity to CFL (Ramos et al., 1996a,b). Accordingly, ConA and CFL recognize the same carbohydrate structures already tested by different methods although the magnitude of interaction may vary for more complex carbohydrate structures (Dam et al., 1998; Ramos et al., 2002). According to western blotting analysis, ConA recognized a high molecular mass component in all protein fractions from the Cratylia floribunda seed flour. Furthermore, it appears exactly at the same position in all protein fractions (figure 3). The band was more evident in the globulin fraction but seed flour also present to a lesser extent in albumin, acidic and basic glutelin and prolamin fractions. No other additional bands were detected within the protein fractions, CFL or in the lines corresponding to the molecular mass markers (see figure 3). This supports the hypothesis that binding of ConA was carbohydrate specific. These results support those obtained by affinity chromatography of CFL with albumins, globulins and acidic glutelins even if they differ for prolamin and basic glutelin fractions. Positive results in western blotting obtained with the latter fractions may be due to the higher sensitivity of the method used rather than in differences in the specificity of CFL and ConA.
In order to extend the analysis of lectin binding to protein fractions, the kinetic interaction of immobilized CFL and soluble proteins from albumin, globulin, prolamin and acidic and basic glutelin fractions was measured in real time by surface plasmon resonance technology. First, CFL was covalently bound to a carboxymethyl dextran layer of the sensor ship. Kinetic analysis of the interaction between CFL and soluble soybean agglutinin was performed to test the lectin activity (figure 4A). CFL, which is known to interact strongly with high mannose oligosaccharides, bound tightly to soybean, a glycoprotein possessing a high mannose oligosaccharide (Dorland et al., 1981) (curve A) and this interaction was partially inhibited by the presence of 100 mM a-methylmannoside (curve B). Kinetic analysis of individual protein fractions provided interesting results. No interaction was observed between CFL and prolamin or basic glutelin fractions while albumin, globulin and acidic glutelin fractions were more or less recognized by the lectin (figure 4B). Profiles yielded with prolamins and basic glutelins were typical of a lack of interaction. Curves obtained with albumin, globulin and acidic glutelins are typical of a weak interaction and this could be explained by a very low concentration of the soluble ligand in the assayed samples. During the association phase, increase in resonance units occurs progressively while in the dissociation phase, decrease in resonance units is more expressive. Despite the performance of the SPR methodology, results obtained by western blotting analysis were not reproduced with the prolamin and basic glutelin fractions. This may be interpreted by differences of sensitivity in the two approaches. The sensitivity of the western blotting analysis was improved by washing of non-specific ligands. RPS is a very sensitive method for bio-interaction analysis but the dissociation is highly favoured if ligand concentration is not ideal or if affinity is not strong. Furthermore, prolonging the dissociation phase with running buffer induces natural dissociation and thus does not allow inhibition assays to be performed. Accordingly, inhibition of the interaction of CFL with albumin fraction with 100 mM a-methylmannoside did not change the kinetic profile at all (figure 4C). It should be noted that kinetic interaction results based in surface plasmon resonance corroborated with those of affinity chromatography, where prolamin and basic glutelin fractions did not bind to immobilized CFL while albumin, globulin and acidic glutelin proteins did.
Because many strategies have failed to detect endogenous lectin receptors, it is expected that lectin ligands should occur at very low concentrations if it is a soluble compound. Detection of such molecules based in their lectin reactivity is heavily dependent on the sensitivity of the method used. In addition to well-explored affinity chromatography principles, combined western blotting and RPS analysis of the protein fractions were used successfully here. Kinetic curves for albumin, globulin and acidic glutelin fractions did seem to reflect the presence of a lectin ligand. Even at very low concentration, some soluble component in these fractions seems to be an endogenous CFL ligand. Accordingly, dissociation is very fast and addition of inhibiting sugar could not improve dissociation (figure 4C). It should be expected that crude extract did not work as well as protein fractions because the later represent more purified preparations. This is why the crude extract was first fractionated before analysis. Alltogether, results from SDS-PAGE, western blotting, affinity chromatography and RPS analysis emphasized the presence of an endogenous lectin receptor within the Cratylia floribunda seed.
Despite the great number of reports describing many biochemical and structural aspect of plant lectins and their increasing utilization as biotechnological tools, many questions concerning their role in the plant kingdom still remain open. As a starting point, lectin distribution in plants and their ultra structural localization addressed the first hypothesis regarding their functions. Accordingly, earlier investigations explored the role of lectins in plant defence against seed predators (insects or fungi) or during seedling development (Etzler, 1998; Naeem et al., 2001). Legumes are still considered as the most important source of lectins. In seeds, considerable amounts of lectin accumulates in the protein bodies forming a package together with well-characterized storage proteins, although lectins are also found in the root hair as in pea plants (Diaz et al., 1989). Otherwise, the carbohydrate binding specificity and the molecular basis of lectin-carbohydrate interaction are now well established mainly for legume lectins (Van Damme et al., 1998). The great gap in lectin research is the detection and characterization of endogenous receptors. As a consequence, some authors have defended the hypothesis that higher plants developed structurally different motifs to recognize foreign glycans based on lectin binding properties (Peumans et al., 2000). References to research on endogenous lectin binding partners are rare in the literature. As a whole, they provided evidences for the interaction of seed lectins with some components of the protein body membranes or with the storage proteins themselves. The fact that these interactions seem to be mediated by ionic strength more than governed by the carbohydrate-binding site of the lectins or by both interactions deserves more detailed investigations.
We have investigated here the presence of an endogenous receptor for the Cratylia floribunda seed lectin. It should contribute to extend results showing that lectins may recognize endogenous compounds through interactions either mediated by their carbohydrate-binding site or by the whole molecule. Assuming that the researched ligand(s) occur(s) at very low concentration, the crude saline extract of the seed flour was first fractionated into albumin, globulin, prolamin and acidic and basic glutelin fractions. As presented here, CFL seemed to bind to some components of the protein fractions. Albumin, globulin and acidic glutelin fractions were shown to contain protein(s) cross reacting with immobilized CFL as explored by affinity chromatography. Displacement of the bound material by changing the pH conditions in affinity chromatography does not clarify whether this interaction is mediated by the carbohydrate binding-site, sustained by salt bridges between the lectin and the protein fraction, or by both mechanisms. However, it is worth noting that the experiments were conducted in 150 mM NaCl solution, which avoids or strongly minimizes electrostatic effects. Furthermore, when eluted with 100 mM mannose instead of the glycine-HCl buffer, albumin fraction was shown to yield a similar absorbance peak (data not shown). Thus, one can assume that interaction of a minor component of this fraction with the lectin column is carbohydrate-mediated.
The nature of the lectin interaction with endogenous molecules is obviously of great interest as these proteins are grouped together based in their most prominent characteristic that is the ability to specifically bind carbohydrates. Evidences that plant lectins interact with classical storage proteins or membrane components of the protein bodies by ionic forces as shown by Rudiger and co-workers could reflect the highly compacted structure of the protein bodies. This would induce natural contacts among the stored proteins, rather than represent a specific characteristic of lectins or the fact that the lectins exist to accomplish this role. Indeed, results from Rudiger and co-workers, cited previously, gave insights into the idea that legume lectins do participate in the maintenance of the protein bodies membrane and most importantly that the assayed lectins were able to bind to some endogeneous glycoconjugate(s), even if ionic strength also seemed to be involved. Furthermore it is not plausible to consider that lectins could be an extraneous molecule in this sub-cellular structure. Chemical affinity among proteins stored in protein bodies ought to be a sine qua non condition to make possible the package of the organelle.
The aim of our work was to get evidence that plant lectins have endogenous receptor(s) for their carbohydrate-binding site. To accomplish this goal, Cratylia floribunda seed flourwas investigated because it possesses a well-characterized glucose/mannose binding lectin. The lectin was shown to bind to different protein fractions prepared from the crude saline seed flour extract and the closely related lectin ConA was shown also to bind to the same soluble molecule(s) present in the C. floribunda protein fractions by western blot analysis. In summary, the results presented here give evidence on the interaction of the seed lectin from Cratylia floribunda with soluble molecules present in the protein fractions obtained from the seed flour and show that this interaction may be mediated by the lectin carbohydrate-binding site.
Acknowledgements: The authors thank IFS (MVR), FUNCAP, CNPq, CAPES/COFECUB program for the financial support. We are indebted to the service commun d'imagerie cellulaire de l'Université de Lille II for providing access to the BiaCore Apparatus.
Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254. [ Links ]
Canziani G, Zhang W, Cines D, Rux A, Willis S, Cohen, G, Eisenberg, R, Chaiken, I (1999) Exploring biomolecular recognition using optical biosensors. Methods 19:253-259. [ Links ]
Dam TK, Cavada BS, Grangeiro TB, Santos CF, Sousa FAM, Oscarson S, Brewer CF (1998) Diocleinae lectins are a group of proteins with conserved binding sites for the core trimannoside of asparagine-linked oligosaccharides and differential specificities for complex carbohydrates. J. Biol. Chem. 273:12082-12088. [ Links ]
Dam TK, Cavada BS, Grangeiro TB, Santos CF, Ceccatto VM, Sousa FAM, Oscarson S, Brewer CF (2000) Thermodynamic binding studies of legume lectins from Diocleinae subtribe to deoxy analogs of the trimannoside core of asparagine-linked oligosaccharides. J. Biol. Chem. 275: 16119-16126. [ Links ]
Diaz CL, Melchers LS, Hooykaas OJJ, Lugtenberg BJJ, Kijne JW (1989) Root lectin as a determinant of host-plant specificity in the Rhizobium-legume symbiosis. Nature 338:579-581. [ Links ]
Dorland L, Van Halbeek H, Vliegenthart JFG, Lis H, Sharon N (1981) Primary structure of the carbohydrate chain of soybean agglutinin. J. Biol. Chem. 256:7708-7711. [ Links ]
Dubois M, Gilles KA, Hamilton JK (1956) Calorimetric method for determination of sugars and related substances. Anal. Chem. 28:350-356. [ Links ]
Einhoff W, Fleischmann G, Freier T, Kummer H, Rudiger H (1986) Interaction of leguminous seed lectins with seed proteins ¾ lectins as packing aids of storage proteins. In: Bog-Hansen TC (ed), Lectins: Biology, Biochemistry, Clinical Biochemistry v. 5, pp.45-52. Textop, Hellerup (Denmark). [ Links ]
Etzler ME (1998) From structure to activity: new insights into the functions of legume lectins. Trends Glycosci. Glycotechnol. 10:247-255. [ Links ]
Gers-Barlag H, Schecher G, Kumar NS, Rudiger H (1993) Protein body membranes as binding partners of lectins. In: Van Driessche E, Franz H, Beeckmans S, Pfuller U, Kallikorm A, Bog-Hansen TC (eds.), Lectins: Biology, Biochemistry, Clinical Biochemistry v. 8, pp.97-100. Textop, Hellerup (Denmark). [ Links ]
Gorocica P, Lascurain R, Hemandez P, Porras F, Bouquelet S, Vázquez L, Zenteno E (1998) Isolation of the receptor for Amaranthus leucocarpus lectin from marine peritoneal macrophages. Glycoconjugate J. 15:809-814. [ Links ]
Laemmli UK (1970) Cleavage of structural proteins during the assemble of the bacteriophage T4. Nature 227:680-685. [ Links ]
March SC, Parikh I, Cuatrecasas P (1974) A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Anal. Biochem. 60:149-152. [ Links ]
Naeem A, Khan RH, Vikram H, Akif M (2001) Purification of Cajanus cajan root lectin and its interaction with rhizobial lipopolysaccharide as studied by different spectroscopic techniques. Arch. Biochem. Biophys. 396:99-105. [ Links ]
Oliveira JTA, Cavada BS and Moreira RA (1991) Isolation and partial characterization of a lectin from Cratylia floribunda Mart. seeds. Ver.. Bras. Bot. 14:61-66. [ Links ]
Peumans WJ, Barre A, Qiang H, Rougé P, Van Damme EJM (2000) Higher plants developed structurally different motifs to recognize foreign glycans. Trends Glycosci. Glycotechnol. 12:83-101. [ Links ]
Ramos MV, Moreira RA, Cavada BS, Oliveira JTA, Rougé P (1996a) Interaction of lectins from the sub-tribe Diocleinae with specific ligands. Rev. Bras. Fisiol. Veg. 8:193-199. [ Links ]
Ramos MV, Moreira RA, Oliveira JTA, Cavada BS, Rougé P (1996b) Structural similarities among Diocleinae lectins. In: Van Driessche E, Franz H, Beeckmans S, Pfuller U, Kallikorm A, Bog-Hansen TC (eds), v.11, pp.44-49, Textop, Hellerup (Denmark). [ Links ]
Ramos MV, Cavada BS, Mazard AM, Rougé P (2002). Interaction of Diocleinae lectins with glycoproteins based in surface plasmon resonance. Mem. Inst. Oswaldo Cruz 97:275-279. [ Links ]
Rudiger H, Schecher (1993) The protein body membrane of soybean seeds as a possible lectin-binding component. In: Van Driessche E, Franz H, Beeckmans S, Pfuller U, Kallikorm A, Bog-Hansen TC (eds) Lectins: Biology, Biochemistry, Clinical Biochemistry v. 8, pp.101-104. Textop, Hellerup (Denmark). [ Links ]
Rudiger H, Siebert HS, Solís D, Barbero JJ, Romero A, Von der Lieth CW, Maurino TD, Gabius HJ (2000) Medical chemistry based on the sugar code: fundamentals of lectinology and experimental strategies with lectins as targets. Curr. Med. Chem. 7:389-416. [ Links ]
Sáez FJ, Madrid JF, Aparicio R, Leis O, Oporto B (1999) Lectin histochemical localization of N- and O-linked oligosaccharides during the spermiogenesis of the urodele amphibian Pleurodeles waltl. Glycoconjugate J. 16:639-648. [ Links ]
Satoh A, Matsumoto I (1999) Analysis of interaction between lectin and carbohydrate by surface plasmon resonance. Anal. Biochem. 275:268-270. [ Links ]
Schercher G, Rudiger H (1994) Interaction of the soybean (Glycine max) seed lectin with components of the soybean protein body membrane. Biol. Chem. Hoppe-seyler 375:829-232. [ Links ]
Van Damme EJM, Peumans WJ, Barre A, Rougé P (1998). Plant lectins: a composite of several distinct families of structurally and evolutionary related proteins with diverse biological roles. Crit. Rev. Plant Sci. 17:575-692. [ Links ]
Wenzel M, Rudiger H (1995) Interaction of pea (Pisum sativum) lectin with pea storage proteins. J. Plant Physiol. 145:191-194. [ Links ]
Wu AM, Herp A, Song SC, Wu JH, Chang KSS (1995) Interaction of native and asialo rat sublingual glycoproteins with lectins. Life Sci. 57:1841-1852. [ Links ]
Zeng X, Murata T, Kawagishi H, Usui T, Kobayashi, K (1998) Analysis of specific interactions of synthetic glycopeptides carrying N-acetyllactosamine and related compounds with lectins. Carbohydr. Res. 312:209-217. [ Links ]
Address to correspondence
Márcio Viana Ramos
Received: 7/10/2002
Accepted: 21/11/2002


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Results 1 to 5 of 32
April 9th, 2013 01:54 AM #1
Unlicensed Midwife Epidemic in my town
I live in a very "granola" town and midwifery/natural birth is a HUGE deal here. The biggest local Women's clinic has almost as many midwives on staff than doctors. Anyway, one of these unlicensed "midwives" was arrested for murder recently after a baby she was delivering died en route to the hospital. Local licensed midwiferies are "saddened" and are saying it could have been prevented if she were properly trained. But the community as a whole is actually rallying behind this woman and trying to raise $15k for her bail. http://projectavalon.net/forum4/show...needs-our-help
I don't think murder/life in prison is appropriate, but this woman (and others like her) should be stopped, and she should be held accountable. Here's a link to the original news story: http://www.wyff4.com/news/north-caro...n/-/index.html
What are your thoughts on this?My cherished daughter, Rowan Jane. ~b. 10/2011~
Sawyer ~ Aven ~ Elowen ~ Sage ~ Eilonwy ~ Eleanor
Morgan ~ Asher ~ ___ ~ ___ ~ Currently trying to fill the blanks...
Trying for #2 in January 2014.
April 9th, 2013 02:13 AM #3
My thoughts on this could fill oceans. I have been following the Rowan Bailey scenario with mute horror (even by pathetic CPM standards the woman was completely untrained, and she also worked as a prostitute). Succinctly, I hope they are prosecuted into oblivion. Nurse-midwifery, college degrees, hospital experience, prescribing privileges, malpractice insurance, transfer protocols and accountability or bust!Blade, MD
XY: Antoine Raphael; Julian Victor
XX: Cassia Viviane Noor
Allaire * Emmanuelle * Honora * Lysandra * Marina * Rosamond * Serena * Sylvie * Thea * Verity / Blaise * Cyprian * Evander * Jules * Laurence * Lucian * Marius * Quentin * Rainier * Silvan
Hayat _ Qamar _ Sahar _ / Altair _ Faraj _ Tariq
April 9th, 2013 02:27 AM #5Senior Member
- Join Date
- Mar 2013
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In the interest of full disclosure for my bias, I am a student midwife with intentions of beginning a homebirth practice after my certification and licensure.
There are several states in the US that full-on prohibit direct-entry (read: non-nurse) midwives from practice. North Carolina is among the most notorious. Direct-entry midwives are illegal there, and as such, no regulations or statutes are in place to ensure that women seeking the care of a DEM are getting any kind of quality care. In other words, even after I'm done with five years of midwifery school, assist at 150 births, and pass certifying exams by the North American Registry of Midwives, I will still be deemed to have inadequate training to receive a license to practice midwifery in North Carolina because I am not a registered nurse.
Any midwife worth her salt, DEM or nurse-midwife, must have appropriate training, certification, and licensure. Non-negotiable. You do not screw around with the lives of mothers and babies. Any midwife who doesn't think that's necessary is, frankly, an idiot. But if legislation is not passed that (a) legalizes the practice of direct-entry midwifery in the first place, and (b) provides oversight of DEMs training, credentials, certification, and licensure, then inadequately trained, unlicensed DEMs are going to keep practicing underground. We have ample evidence from history to support that, when women are desperate enough to keep within their control anything relating to their reproduction, they're going to seek out whatever avenues they can to get it, legal or not. If a woman wants badly enough to have her baby with a midwife at home, she's going to, and if that means she's got to use an unlicensed midwife, many will. The fewer trained hands there are to catch these babies, the more this is going to happen.
I don't know enough about the circumstances of this birth to make a judgment call on whether the midwife acted improperly or if this was a circumstance that would not have been able to be helped no matter what. There are more scenarios that point to the midwife's culpability in this death than not, but I don't have enough data to say so or not. If she did contribute to the baby's death in any way, I would love nothing more than for her to fry for it. The death of any newborn is horrific, though, no matter the cause.
At an absolute minimum, this woman needs to go down for unlicensed practice of midwifery and never. Lay. Her. Hands. On. Another. Laboring. Mother. Or. Infant. Again.
Last edited by shyshutterbug; April 9th, 2013 at 02:35 AM.
April 9th, 2013 02:31 AM #7
Rowan (well, not her real name)
It's apparently a huge issue here... and it's very frustrating how many people are rallying behind her and acting like it wasn't her fault. I love my city, but sometimes I feel like I'm living in a bad Portlandia episode.My cherished daughter, Rowan Jane. ~b. 10/2011~
Sawyer ~ Aven ~ Elowen ~ Sage ~ Eilonwy ~ Eleanor
Morgan ~ Asher ~ ___ ~ ___ ~ Currently trying to fill the blanks...
Trying for #2 in January 2014.
April 9th, 2013 03:09 AM #9
We had a similar situation in Australia recently (in Adelaide, where I live). A midwife named Lisa Barrett was present at three home births where babies died. As far as I'm aware, she has never been charged and she claimed to be working as a "birth advocate" rather than a midwife. Coronial inquests found that all three deaths were preventable. Two of the births were high risk - one was breech and the other was twins (one of the twins survived).
Here's a link to the most recent news headline (from February 1, 2013):
http://www.adelaidenow.com.au/news/s...-1226566712748~ Violet Elizabeth Rose ~


text:
my november guest
My Sorrow, when she's here with me,
Thinks these dark days of autumn rain
Are beautiful as days can be;
She loves the bare, the withered tree;
She walked the sodden pasture lane.
Her pleasure will not let me stay.
She talks and I am fain to list:
She's glad the birds are gone away,
She's glad her simple worsted gray
Is silver now with clinging mist.
The desolate, deserted trees,
The faded earth, the heavy sky,
The beauties she so truly sees,
She thinks I have no eye for these,
And vexes me for reason why.
Not yesterday I learned to know
The love of bare November days
Before the coming of the snow,
But it were vain to tell her so,
And they are better for her praise.
Robert Frost
by Dakota at 5:05 PM 8 comments:
Hope you had a spellbinding All Hallows Eve !
I was Baby Jane Hudson with a dash 'o' haunted doll ✯ ☽ ✯
by Dakota at 10:43 PM 7 comments:
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A Bosnian court on Thursday convicted a man who opened fire on the U.S. embassy of terrorism and sentenced him to 18 years in prison. Mevlid Jasarevic shot at the embassy in Sarajevo for 50 minutes with an automatic rifle, injuring a local policeman before authorities shot him in the leg and arrested him. He said in a video made before the Oct. 28, 2011, shooting that he wanted to force NATO to pull out of Afghanistan and for Bosnia to stop harassing his Islamic sect.
Alleged accomplices Emrah Fojnica and Munib Ahmetspahic were acquitted. The court said the prosecution did not prove the three were an organized terrorist group. The prosecutor's office said it will appeal the acquittal. (continue reading...)(...more)


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How do I reserve a study room?
Customers can reserve the private study rooms on the second floor of the Lee Road.
- Reservations can be made by phone at (216) 932-3600 or in person at the Lee Road branch, 2345 Lee Road in Cleveland Heights.
- Reservations can be made for 2 hours at a time, up to two weeks in advance.
- Reservations are limited to one reservation per day per person.
- Reservations will be held for 15 minutes. After 15 minutes, the customer forfeits the reservation and the room will be released.
- Rooms that are not reserved will be available on a first come, first served basis.
- Customers may stay beyond their reserved time if there is no one else waiting to use the room.
- No more than five people are permitted in a room at a time.


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Arthritis health centre
Coping with psoriatic arthritis
The aching joints and draining fatigue of psoriatic arthritis can affect all aspects of a person's everyday life.
Get tips on coping with psoriatic arthritis.
Psoriatic arthritis: Symptoms can change
Around one in five people with psoriasis develop psoriatic arthritis. Like other forms of arthritis this condition results in painful, swollen joints and, if not treated early, can lead to severe joint damage.
Psoriatic arthritis symptoms can vary in intensity among people or even in the same person over time. Symptoms can come and go, sometimes seemingly on a whim. This aspect of the disease can make it incredibly difficult to manage.
Experiencing at unpredictable moments not being able to walk or finding that your hands just don't work in the middle of something is hard.
Psoriatic arthritis triggers
There is no doubt about it: psoriatic arthritis can take a toll on your emotional and physical health, from the embarrassment you might feel about your skin lesions to your swollen joints and other painful symptoms.
Although much about psoriatic arthritis remains unknown, studies have discovered that there are some possible triggers behind flare-ups, including:
- Too much alcohol
- Smoking
- Stress
Stress definitely seems to play an important role in the onset and severity of both psoriasis and psoriatic arthritis symptoms. Although doctors aren't sure why some people with psoriasis get psoriatic arthritis, evidence suggests that the more severe the psoriasis, the more likely you are to develop psoriatic arthritis.
However, don't be discouraged, with the right treatment the vast majority of people with psoriatic arthritis do find relief and a reduction of their symptoms
Coping with psoriatic arthritis: Get medical help
If you have psoriasis and also have any of the symptoms of psoriatic arthritis, one of the most important first steps is to get the medical help you need.
Start by talking to your GP. They can then identify the right treatments to address your unique needs, and refer you to a specialist such as a rheumatologist or physiotherapist if necessary.
Coping with psoriatic arthritis: Healthy lifestyle
Though exercise might be the last thing you feel like doing with your sore and swollen joints, regular physical activity may actually make it easier to cope with psoriatic arthritis symptoms.
It is a common misconception that patients think they will hurt their joints if they exercise. Many studies regarding exercise and arthritis show positive results.
A regular exercise programme may help alleviate stiffness and strengthen the muscles that surround your inflamed or damaged joints. Stretching and resistance exercises can help improve the range of movement of affected joints.
There are exceptions, of course, like running hard on an extremely inflamed knee or ankle. In general, consistent aerobic conditioning yields positive results, leading to stronger muscles around joints, emotional wellbeing, and less disability. Yoga and tai chi may be helpful.
Finally, exercising and eating a healthy diet also helps you maintain a healthy body weight, reducing joint stress.
Yet even with the healthiest intentions, you may sometimes find your psoriatic arthritis symptoms get in the way of eating healthily, and exercising. Don't be deterred, try these tips:
- If your swollen joints don't fit in traditional exercise trainers, look for shoes with extra room in the toe for added comfort. Shoe inserts, including orthotics or heel pads, may also make exercising more comfortable
- If you can't find a way to exercise that doesn't hurt, ask your GP whether physiotherapy is right for you. A physiotherapist can help you design an exercise program that works with, not against, your psoriatic arthritis
- If your psoriatic arthritis is making your hands stiff and painful, or if you're feeling extremely fatigued, the idea of making a healthy meal can be overwhelming. Make sure you have healthy options, like healthy frozen meals or pre-cut fruit and veg, on hand, so your diet won't suffer when your psoriatic arthritis symptoms get you down


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April 9, 2012 at 6:20 pm #11258
First of all – the custom widget areas – there is no way to have entire custom sidebars per page, right? Because a custom widget area is being ADDED to the existing global widgets, I don't want that – I don't want to add all global widgets to the custom widgets!! But anyway…
Now, my issue is with the custom widget areas. I've added a custom area, for 1 page, and added 2 text widgets there. The first text widget is displayed on http://emeastudio.co.uk/wordpress-web-design/ – it's the first sidebar widget…
The problem?
http://emeastudio.co.uk/wordpress-web-design/ (Purchase code hidden if logged out) / – look at the sidebar on that page.
Why is it a link to itself?April 9, 2012 at 7:30 pm #71161
Hi xorred,
If I understand your first paragraph correctly, you're asking if you can have a custom sidebar for each page. So for example, http://emeastudio.co.uk/wordpress-web-design/ would have it's own sidebar with its own widgets and http://emeastudio.co.uk/elearning-lms/ would have its own sidebar with its own widgets. Is this correct? If so, the answer to your question is yes.
In order to setup a custom sidebar, you would go to Angular > Theme Optoins > Sidebar then select the page that should receive a new widget area and click the + icon. After doing that you would go to Appearance > Widgets > Page: Whatever Your Page Name Is and your widgets.
As you noted above, by default you have some widgets that are displayed on every page. If you prefer not to have the same widgets displayed on every page you can simply delete those from under the "Displayed Everywhere" sidebar. Once you do that then the global widgets will disappear and you'll have only the widgets from the custom sidebar you created.
As far as the widget being a link to itself, I wouldn't be able to tell you the cause unless I saw the back-end. From the front-end, it appears you used the Custom Menu widget?
Hope this helps!
MyaApril 11, 2012 at 3:36 pm #71162
The problem is that this page links to itself, yes. There should NOT be a link to page X on page X, right? That is a bug. I can't post the wp admin credentials here and I think the theme dev should look into it.
To replicate: Have a parent page, link to a child page of it in the sidebar (text widget link).
The child page links to itself on the sidebar. That's a problem. It should not happen. Google will enter into a loop and punish that page!April 11, 2012 at 9:32 pm #71163
Hi Xorred,
Okay, I see now. Sorry, the "text widget" aspect was throwing me off.
The theme has a built-in sub-navigation that automatically appears on the parent page as well as any child pages as soon as you create a child page. The fact it links to itself even though you're on that page is not a bug. If you notice your main navigation behaves the sames.
Example from the demo: http://kriesi.at/themes/angular/template-files/
That URL takes you to the Template Files page but you still click the Template Files link in the main navigation.
As far as Google goes, I know nothing about pages linking to themselves causing Google to enter into a loop and punish the page. Mind you I'm not a SEO expert I only go with what Google's Webmaster Guidelines say.
MyaApril 11, 2012 at 10:44 pm #71164
I am using the Woo plugin they released recently to manage sidebars now…April 11, 2012 at 10:57 pm #71165
Hi Xorred,
Okay. Just be aware that the WooDojo was just released and Kriesi has yet to test. You may experience some strangeness. If you want to keep up-to-date there's already a thread started here: https://kriesi.at/support/topic/ (Purchase code hidden if logged out)
The topic 'Strange sidebar behavior' is closed to new replies.


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Maple Spices & Seasonings
Here are quite possibly the best seasoning and spices you've ever tasted. Imagine, if you can, a medley of salts, peppers and other spices blended with maple sugar – giving your dishes just a hint of pure maple flavor. Whether it be for steaks on the grill or an apple pie, you'll find these medleys to be a wonderful pantry item for anyone who enjoys cooking or baking.
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Lawn and yard maintenance machinery, namely, lawnmowers, commercial mowing equipment, namely lawn mowers and grass collectors
Business in WAUWATOSA, WI
Business in Munnsville, NY