text:
Grateful Dead
so sad...turn that frown upside down :)
Nothing left today but smile smile smile.....
I hope your migraine's gone!!
my head is throbing
my head is pounding
I try to smile
but can only frown
like being a sad clown
I can not shake this frown
as my head keeps pounding
I continue my frowning
and the twinkle in my eye`s
starts to slowley die
drownding in my oun tears
Sunlight splatters
Nothing matters
Without Love
Take me and awake me
Don't forsake me
With your Love
And I'll try, to do the best I can
To get by, and give ourselves a chance
It's time to make a plan
And leave it up to Love
Reason tattered
I've been captured
By your Love
Give your dreams
A set of wings
And give yourself a shove
And you'll find, that the world was made for you
If you try, there's nothing you can't do
So to your heart be true
And let Love see you through
a dream is only a dream
or is it
for life is the aftermath of our dreams
and our dreams keep us in the fullest of life
a man must dream or he will never achieve
almost all we do
are the reflections of a dream
There is day, and there is night
There is wrong, and there is right
Some find peace, while others fight, oh yeah
In the cities, and in the towns
There are smiles, and there are frowns
Some see the priest, I'll take the clowns, oh yeah
You'll be up, and you'll be down
You'll be lost, and you'll be found
Look for Love, it's all around you, yeah
There is sunshine, and there is rain
There is laughter, and there is pain
May your love, always remain, oh yeah
All I see is illusion, the hands of man have built
Don't give in to confusion, or be haunted by guilt, oh yeah
in the shadows
of the moon
I heard a great boom
like the sounds of thunder
coming from down under
I stand and wonder
what kind of blunder
could posibley come from
down under
could this just be thunder ?
do`es this make you wonder ?
I have long hair
and I just don`t care
watch out for that bear
I must say
do you see the tree`s sway
we better stay away from here
is what I hear
for the great beast
has no fear
don`t you hear what i say
look at the tree`s sway
we better stay away from here
for the great beast has no fear.
Plenty of room for jammin too. Thanks for trying it. And a big thanks to Stuman for suggesting this forum. My favorite.
Two times through each verse, right? Sorted! Fun and bouncy!
Conversation is always more interesting than recitation, so speak your mind and not someone else's.


text:
Multiple insulin injections each day are a way of life for those with type 1 diabetes. But did you know that where you inject the insulin makes a big difference in the absorption level and effectiveness of the insulin?

Here are 5 tips that will help ensure that your injections are working hard for you.

1. Give injections in the abdomen, thighs and back of the upper arm whenever possible.

Insulin is most rapidly absorbed when injected in the abdomen, followed by the upper arm and thigh area. Injections in your hip and buttock areas are more slowly absorbed. Never inject within two inches of your navel.

2. Choose a slightly new location for each injection.

This is called site rotation. For example, if you give all of your injections in the abdomen, take note of where your last injection was given and move the next one about an inch to one side or the other. Continue to move the injection site until you've covered all the available sites before starting a new area.

3. Always inject insulin into fatty tissue instead of muscle.

That's why the abdomen, upper back of the arms and outer thigh are preferred. These areas are easy to reach and have ample amounts of fatty tissue (called subcutaneous fat). These areas also reduce the risk of injecting insulin too close to a large blood vessel or nerve.

4. Give your injections in the same general area at the same time each day.

For example, take your morning insulin in your abdomen and your afternoon or evening insulin in your arm. This consistency helps your body better absorb the insulin over random injections.

5. Keep accurate records of your site rotation.

This will help you avoid injecting the same area repeatedly. Doing so is likely to result in the development fat deposits that can make your skin look lumpy and delay the absorption of insulin. The Cleveland Clinic offers a helpful site rotation map for each area of the body that you can print and use to check off where you last injected.

Source:

Insulin Routines. American Diabetes Association. http://www.diabetes.org/living-with-diabetes/treatment-and-care/medication/insulin/insulin-routines.html

subset: commoncrawl


text:
NEW YORK -- Maybe Casper Wells' problems at the plate are as simple as this:
He can't see.
Wells went 0 for 7 with four strikeouts in Saturday night's 18-inning loss to Arizona. He also took the loss on the mound when the Phillies ran out of pitchers.
But the troubling part of Wells' short time with the Phillies is this: He is 1 for 23 with eight strikeouts.
On Sunday, Wells went to manager Ryne Sandberg and admitted that he's having trouble seeing the ball -- not just at the plate, everywhere, even in the outfield.
"The ball looks blurry to him," Sandberg said.
The Phils placed Wells on the disabled list Monday and brought up utility man Pete Orr from Triple A Lehigh Valley.
Wells will be examined by eye specialists this week in Philadelphia.
GM Ruben Amaro Jr. said Wells had LASIK surgery last fall and has had some complications.
"He really hadn't told anybody about it until now," Amaro said. "He went to Ryne and said that he was fearful that he was going to get injured because he wasn't able to pick up the ball, and he wasn't able to play at an optimal level because he couldn't see.
"You don't know if he was struggling or not seeing the ball well, but obviously he was having those issues, so that could be part of it. He wasn't making excuses. He just wants to get to the bottom of it."
Overall this season, Wells has played for three teams and is hitting .128
(12 for 94) with 31 strikeouts.
Brown getting close
Domonic Brown was out of the starting lineup for a second straight game Monday night. He has battled some soreness in his right Achilles tendon since hitting the first base bag awkwardly Friday night.
Sandberg said the Phillies were not considering the disabled list for Brown.
"Just day to day," Sandberg said.
Brown was available for pinch-hitting duty.
Volquez, maybe?
Far out of contention, the Phillies over the final weeks of the season are evaluating a number of players and their possible roles in the future. Roger Bernadina is getting looks in the outfield along with Darin Ruf. Cody Asche is being evaluated at third base. Roy Halladay's health and effectiveness are being gauged. Even free-agent-to-be Carlos Ruiz is under the microscope.
Could Edinson Volquez be next? The right-hander has been released by San Diego.
"We've talked about it internally," Amaro said.
Volquez went 17-6 with a 3.21 ERA for the Reds in 2008. He had Tommy John surgery the next year and has struggled to replicate that success. He had a 6.01 ERA in 27 starts for the Padres this season.
In 2010, Volquez was found in violation of MLB's policy against performance-enhancing drugs and served a 50-game suspension.
Harvey shut down
Speaking of Tommy John surgery, the Mets shut down hard-throwing phenom Matt Harvey with a partially torn ulna collateral ligament on Monday (see story). Rest has been prescribed, but surgery is a possibility. If Harvey needs ligament-replacement surgery (Tommy John), he will likely miss next season.
The 24-year-old right-hander is 4-0 with a 1.08 ERA in five career starts against the Phillies. He has allowed just 15 hits and eight walks while striking out 38 in 33 1/3 innings against the Phillies.
Harvey was supposed to face the Phillies on Thursday. Right-hander Carlos Torres will fill that spot.
In the fraternity of baseball, no one is ever happy to hear of an injury, even when it happens to an opponent.
"He's a bright star, one of the best we've seen this year," Sandberg said. "You hate to see that. It's a shame."


text:
Professor Admits to Harassing Student, Keeps Job Anyway
Even though Jeffry Mathis, an adjunct professor in the Department of Kinesiology at San Jose State University, admitted that he had acted inappropriately towards a female undergraduate nine months ago, he has not been disciplined by the university and is still listed on the faculty. The student, who remains anonymous as she is still studying at SJSU, tells KNTV that she feels that the university has indeed "failed her."
As the news station reports, the student had asked to meet with Mathis last August to discuss why she had received a D+ grade in his class. Mathis said that her grade was due to plagiarism; the student disputed this. But then, as the student details, this occurred:
He looked at me and touched me and said, 'How do you want to better your grade?" He kept coming closer to me and my body completely shut down. He continued to touch me and try to talk about the ways that I could better my grade.
According to the student, Mathis continued his advances and blocked the stairs when she attempted to leave. She was able to do so, after he had made more advances. Within hours, the student filed an incident report with the university police.
Authorities investigated Mathis for false imprisonment and sexual battery. He claimed that the student had consented to his actions and denied any criminal wrongdoing; no charges were filed against him on the grounds that there was "insufficient evidence," according to a copy of the confidential investigation report written by SJSU and obtained by KNTV. However, an email that Mathis sent the student the day after the incident (which was also obtained by KNTV) suggests a different version of events. In the email, Mathis wrote:
"I've been thinking about last night and I have come to the conclusion that I made a terrible mistake in how I handled that situation….I will change your grade to a B- for free, because it is the right way to handle this."
SJSU has declined to comment about the case except to confirm that Mathis had not gone "through a formal disciplinary hearing and that the university did not discipline Mathis for what happened in his office."
KNTV Chief Investigative Reporter, Tony Kavaleski, attempted to speak to Mathis after a class in March and was informed by the professor that "Well, unfortunately, because of what I was told by both HR and the [police department] here, I'm actually not allowed to talk about it. So, I follow directions."
SJSU Says Professor "Did Violate His Professional Responsibility"
What seems to be insufficiently acknowledged in this case is that Mathis, as a professor, was in a position of power in relation to the student. She had gone to speak to him about her grade; as a professor, he was in charge of assigning her a grade in his class. SJSU's investigation report indeed states that a faculty member has a "duty not to allow situations to develop where a student could feel compelled to 'consent' to activities they would not otherwise agree to in order to be successful in his class."
Beth De Lima, a human resources expert interviewed by KNTV, emphasizes that the fact that Mathis has not been disciplined is "surprising, simply because we have acknowledgment by the instructor." She also notes that, because the university has not taken any action against Mathis, it is "in essence … condoning the behavior." SJSU is also potentially out of compliance with its own professional code of conduct policy, which states that faculty members should "assure that their evaluations of students reflect only matters relevant to the students' academic performance" and "insure that their professional contacts with students are free from exploitation, harassment, or discrimination."
Of the incident, the student herself says that "This is something I have to go through that I never thought I would have to go through. Especially in my college career."
While SJSU seems to be withholding judgment about Mathis' inappropriate behavior towards the student, it has exercised its authority in one area. The university overruled Mathis about the grade change and the student still has a D+ in the class. As other reports about sexual harassment and assault on campuses public and private in the U.S. show, American colleges and universities have a long way to go before they get truly serious about the safety and well-being of their students.
Photo from Thinkstock


text:
Matuidi set for St Etienne
By Patrick Haond & Laurent Picard
Last Updated: 01/01/70 1:00am
Troyes midfielder Blaise Matuidi is being tipped to join St Etienne.Troyes midfielder Blaise Matuidi is being tipped to join St Etienne.
Matuidi has been strongly linked with moves to Auxerre and Bordeaux, but is reportedly set for Les Verts.
According to L'Equipe, St Etienne will sign the 20-year-old for ?4-5million (£2.7-3.4million).
The France Under 21 international is understood to be signing a four-year deal with Laurent Roussey's side.
Meanwhile, Loic Perrin has been confirmed as St Etienne's new captain, replacing Julien Sable.


text:
Why See Eminem & Rihanna?
Eminem and Rihanna - two of the biggest names in music today - join forces for the Monster Tour. As one of the most talked-about double-headliner tours of the year, this six-date tour will feature the two fiery artists collaborating on stage in some of their most popular hits, both as solo artists and ones they've created together.
The tour will include stops in Detroit, Mr Mathers' hometown, and New York City as well as epic after parties with performances by some of Riri and Eminem's muso friends (you'll need a separate ticket for those sadly!). With an exclusive range of merchandise developed by indie label Trapstar, this is one sold-out summer tour you won't want to miss - and we have tickets!
Additional Information
Performance date: 16 August 2014
Additional Information
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Keep up to date!
Straight to your inbox
Please note: The term MetLife Stadium and/or Eminem & Rihanna as well as all associated graphics, logos, and/or other tradermarks, tradenames or copyrights are the property of the MetLife Stadium and/or Eminem & Rihanna and are used herein for factual descriptive purposes only. We are in no way associated with or authorized by the MetLife Stadium and/or Eminem & Rihanna and neither that entity nor any of its affiliates have licensed or endorsed us to sell tickets, goods and or services in conjunction with their events.


text:
Just a few years ago, Mary-Chapin Carpenter was a staple on the respected Washington, D.C., folk scene, the launching pad for Emmylou Harris, among others. Freshly graduated from Brown University with a degree in American civilization, she found herself in the early Eighties rattling around the capital mope scene, strumming covers ranging from Billie Holiday to Fats Waller to James Taylor in smoky Washington clubs like the Birchmere, Gallagher's, and Food for Thought, where she played for tips. A daytime gig as a grants consultant for an arts organization kept her bills paid.
When CBS signed her in 1987 and issued her debut Hometown Girl, the serious-minded Carpenter paid heed to the old showbiz caveat: She didn't give up her day job.
"I was scared at quitting," Carpenter concedes in a phone interview from her D.C. home. "There's no guarantee that you will succeed. A record deal doesn't mean you are set for life. People still register shock at that reality."
Certainly, Hometown Girl wasn't the jackpot that would liberate her from the grind of working days and playing nights. The ballad-laden LP, while widely lauded by critics, National Public Radio stations and college kids, sold just 20,000 units. It did, however, accomplish two things. In addition to gaining her a spot as an opening act for such out-of-the-mainstream country stars as Emmylou Harris, the Desert Rose Band, and Merle Haggard, the album exposed the folksinger's elegantly earthy songwriting abilities. Shortly after the release of her second album State of the Heart and the three chartmakers from the LP--"How Do," "Never Had It So Good," and "Quittin' Time"--the giant songwriting factory SBK signed the Princeton, New Jersey, native.
"It was the greatest feeling, pure validation," the normally reticent Carpenter exclaims. "It was somebody telling you, `We believe in what you're doing.' I'm very proud of the writing deal."
The compact allowed Carpenter to finally--albeit grudgingly--give up her day job.
On the other hand, Carpenter admits, it is reassuring to have that steady job. And it doesn't bother the brainy artist that her bread is amply buttered by Nashville.
"I'm flabbergasted and pleased to be embraced by country radio. The thing about country music is that it is composed of really good songs, not just the pedal steel or a cowboy hat. It is a tradition of songs that mean something. [Songwriter] Guy Clark is a poet. Do me a favor," says Carpenter, warming to the subject, "put on a Marshall Crenshaw album, then segue into a Foster and Lloyd. We're talking a state of mind, not just accessories."
But Carpenter's stubbornly individualistic brand of balladry does stick out among country's traditional-oriented masses. And how long the honeymoon lasts before bowing to irreconcilable differences could be the subject for an office pool. Like the eccentric k.d. lang, there's the possibility she might sing herself off country radio's conservative playlists. Even her resounding success at the Country Music Association awards hootenanny, where she stole the show with "You Don't Know Me, I'm the Opening Act," might have miffed some pompadour'd Nashville power brokers. A roundhouse punch at the business' puffed and haughty headliners, the number produced a standing ovation. It wasn't the sort of "get along, go along" thing they're used to down 'round Tennessee. Yet Carpenter wasn't concerned with ruffling feathers at the time, just with battling a nagging case of stage fright.
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"I was scared to death. I thought I was going to crumble. It wasn't my choice of song. It was the producers'. I was nothing short of astounded at the response."
Carpenter would seem to need all the positive exposure she can scrape up for the country-music long run. Her new album, the splendid and sultry Shooting Straight in the Dark, is a far cry from the sort of stuff spinning on C&W stations. Carpenter possesses a cool and creamy alto that can produce the purest lament, like the lovely "What You Didn't Say," or boil up the bayou, as on the Cajunesque "Down at the Twist and Shout." But don't expect the independent-minded Carpenter to kowtow to the bola-tied brass along Music Row. With her day job an ever-fading memory, Carpenter concludes, "Nobody makes me do anything I don't want to do."
Mary-Chapin Carpenter will perform at Scottsdale Center for the Arts on Sunday, December 2. Showtime is 7 p.m.
"Country music is composed of really good songs, not just a cowboy hat. We're talking a state of mind, not just accessories.


text: Photo, facebook [ Update On Coheed And Cambria Bassist Michael Todd By Dubba G July 12, 2011 Things are not getting any better for Michael Todd! Read More Category: Music Tags: coheed and cambria, michael todd, rock star arrested Send to a friend! Share on Facebook Share on Twitter Pin it! Reddit This! Share on Tumblr!


text:
|Número de publicación
|Tipo de publicación
|Número de solicitud
|Fecha de publicación
|26 Dic 2000
|Fecha de presentación
|10 Oct 1996
|Fecha de prioridad
|10 Oct 1996
|También publicado como
|Número de publicación
|Donald Elliott Brooks
|Cesionario original
|University Of British Columbia
|Exportar cita
|BiBTeX, EndNote, RefMan
|Citas de patentes (50), Otras citas (26), Citada por (49), Clasificaciones (15), Eventos legales (4)
|Enlaces externos: USPTO, Cesión de USPTO, Espacenet
Quantitative analysis of cells and analytes in fluid samples, particularly bodily fluid samples, often provides critical diagnostic and treatment information for physicians and patients. Immunological testing methods (Kennedy, D. M. and S. J. Challacombe, eds., ELISA and Other Solid Phase Immunoassays: Theoretical and Practical Aspects, John Wiley and Sons, Chichester (1988)), which take advantage of the high specificity of the antigen-antibody reaction, provide one approach to measurement of analytes. Immunoassays which provide a quantitative measurement of the amount of an analyte in a sample often use complex, multistep procedures and expensive analyzers available only in a laboratory setting. Immunochromatographic assays, such as those described in GB 2,204,398A; U.S. Pat. Nos. 5,096,837, 5,238,652, and 5,266,497; Birnbaum, S. et al., Analytical Biochem. 206:168-171 (1992); Roberts, M. A. and R. A. Durst, Analytical Chem. 67:482-491 (1995); and Klimov, A. D. et al., Clinical Chem. 41:1360 (1995), are simpler. Immunochromatographic assays rely for their interpretation on the observation of colored reaction products, usually colored particles, in a particular region of a membrane. To obtain a quantitative measure of the amount of an analyte detected in an immunochromatographic assay, the amount of colored particles is analyzed.
Measurement by optical means of the amount of particles accumulated in one or more regions of a membrane is usually performed by reflectometry or densitometry. In these techniques, a light beam is either reflected or transmitted through the colored portion of the membrane, and the light intensity reaching a detector is measured. Other methods include obtaining, with a video camera, the image of the membrane illuminated with transmitted or reflected light, and performing image analysis on a frame of the video record. Although image analysis software allows considerable manipulation of the image to optimize sensitivity in detection of the particles, these methods suffer from light scattering by the membrane fibers in which the particles are imbedded. The multiple scattering that results obscures the optical signal produced by the particles and dramatically increases the noise level in the measurement.
The current invention pertains to methods for quantifying the amount of an analyte in a membrane, in which the membrane is rendered transparent by a clearing agent, and the amount of analyte is measured using an optical means. The membrane can be rendered transparent by wetting the membrane with a clearing agent that has a refractive index (nD) that is approximately equal to the refractive index of the substance from which the membrane is made. For example, a nitrocellulose membrane can be cleared with polyvinylpyrrolidone, polyethleneimine, or benzyl alcohol. Alternatively, the membrane is rendered transparent by applying to the membrane a dissolving agent that dissolves the membrane to form a clear background. For example, a nitrocellulose membrane can be cleared using polyethylene glycol as a dissolving agent. The amount of analyte in the membrane is measured using an optical sensor, such as a sensor that measures fluorescence or light scattering. The analyte can be labelled with a fluorescent label or with detectable particles, such as inorganic particles, organic molecules, liposomes, or organic polymer latex particles, in order to facilitate quantification of the analyte.
Rendering the membrane transparent facilitates detection of the analyte and enhances accuracy and sensitivity of the quantification of the particles, by eliminating or minimizing light scattering by the membrane fibers, and thereby decreasing the noise level caused by background interference in the measurement. The methods are simple, rapid, and can be used for a wide variety of analytes and membranes.
The FIGURE is a graphic representation of the relative signal intensity for various concentrations of latex particles in cleared and in uncleared nitrocellulose membranes.
The current invention pertains to methods of optically detecting and quantifying analytes in a membrane. As described herein, the accuracy and sensitivity of quantification of analyte in a membrane with a light scattering or fluorescence means is enhanced by rendering the membrane transparent. The term, "analyte," as used herein, refers to the molecule, compound, particle, cell fragment, cell, or biological entity for which the amount will be quantified. Examples of analytes include proteins, such as hormones or enzymes; glycoproteins; peptides; small molecules; polysaccharides; antibodies; nucleic acids; drugs, including drugs of abuse; toxins; inorganic particles such as colloidal gold particles; viruses or virus particles; bacteria; whole cells; portions of bacteria or cells; and other compounds. More than one analyte can be quantified. Also or in addition, a control analyte can be quantified.
To perform the methods of the invention, a membrane of interest is provided, having therein the analyte to be quantified (if any is present). The membrane is made of a substance having the capability to be rendered transparent. The membrane can be translucent (i.e., is capable of allowing diffuse light to pass through), or opaque (i.e., incapable of allowing light to pass through). Examples of membrane substances include: cellulose, cellulose nitrate, cellulose acetate, glass fiber, nylon, polyelectrolyte ion exchange membrane, acrylic copolymer/nylon, and polyethersulfone. In a preferred embodiment, the membrane is made of cellulose nitrate, cellulose acetate, glass fiber, or nylon. The membrane can consist of more than one substance: for example, it may consist of nitrocellulose with a Mylar™ backing. If the membrane is made of more than one substance, each substance should either be transparent, or be capable of being rendered transparent.
Analyte "in" or "held in" the membrane includes analyte present on the surface of the membrane, as well as analyte within the substance of the membrane. The analyte can be held in the membrane by adhering to the membrane (e.g., to membrane fibers). Alternatively, the analyte can be trapped within pores or interstices of the membrane. The analyte can also be held in the membrane by applying the analyte in a fluid to the membrane, and then allowing the membrane to dry.
In another embodiment, the analyte is held in the membrane by binding of the analyte to an analyte-binding agent which is immobilized in the membrane. An analyte is "bound" to an analyte-binding agent if it is physically or chemically attached to, adhered to, or associated with, the analyte-binding agent. Analyte-binding agents include antibodies specific for the analyte, receptors that specifically bind to the analyte, genetically engineered binding reagents, bacterial or viral adhesions, aptimers, chelators, binding reagents generated by combinatorial chemistry, or other molecules that bind to the analyte. For example, the analyte can be bound to the membrane by interaction of the analyte with an antibody that is immobilized in the membrane. The term, "antibody," as used herein, encompasses both polyclonal and monoclonal antibodies, as well as mixtures of more than one antibody reactive with an epitope of interest (e.g., a cocktail of different types of monoclonal antibodies reactive with the peptide). The term antibody is further intended to encompass whole antibodies and/or biologically functional fragments thereof, chimeric antibodies comprising portions from more than one species, humanized antibodies, human-like antibodies, surfaced antibodies, and bifunctional antibodies. Biologically functional antibody fragments which can be used are those fragments sufficient for binding of the antibody fragment to the analyte of interest.
The analyte can be labelled to facilitate detection. Examples of such labels include luminescent labels; phosphorescent labels; colorimetric labels, such as dyes; or fluorescent labels. Representative fluorescent labels include fluorescein, fluorescein isothiocyanate (FITC), rhodamine, tetramethyl rhodamine isothiocyaniate (TRITC), Texas red, phycoerythrin, or other fluorochromes. The analyte can be directly labelled, such as by attachment of a fluorochrome to the analyte. Alternatively, the analyte can be indirectly labelled, such as by labelling of the analyte with a reporter molecule (e.g., biotin, digoxigenin), which can be recognized by a reporter-binding molecule (e.g., avidin, streptavidin, or digoxigenin antibody). The reporter-binding molecule is in turn attached to a fluorescent label. The analyte can also be labelled with an antibody that specifically binds to the analyte. The analyte-binding antibody can be conjugated to a fluorochrome. Alternatively, it can be labelled through the use of a secondary antibody conjugated to a fluorochrome, where the secondary antibody recognizes and is specific for the primary, analyte-binding antibody; or through the use of streptavidin-biotin. conjugates, where the primary, analyte-binding antibody is recognized by a biotinylated secondary antibody, which interacts with a streptavidin fluorochrome.
In another embodiment, the analyte can be labelled with a detectable particle. A "detectable particle," as used herein, is a particle which can be detected using an optical sensor.
An "optical sensor" is a sensor that detects electromagnetic radiation method based on the measurement or estimation of a characteristic of light, such as light intensity, reflection, refraction, polarization, fluorescence lifetime, or light of a particular wavelength. Examples are fluorescence intensity measurements, light scattering intensity measurements, analysis of images from optical or fluorescence microscopy, densitometry, and reflectometry.
The analyte is bound to the detectable particle in an appropriate manner, such as by binding of the analyte to an analyte-specific antibody coated on the surface of the particle. Examples of detectable particles include colloidal gold particles; colloidal sulphur particles; colloidal selenium particles; colloidal barium sulfate particles; colloidal iron sulfate particles; metal iodate particles; silver halide particles; silica particles; colloidal metal (hydrous) oxide particles; colloidal metal sulfide particles; colloidal lead selenide particles; colloidal cadmium selenide particles; colloidal metal phosphate particles; colloidal metal ferrite particles; any of the above-mentioned colloidal particles coated with organic or inorganic layers; protein or peptide molecules; liposomes; or organic polymer latex particles. In a preferred embodiment, the particles are polystyrene latex beads, and particularly, polystyrene latex beads that have been prepared in the absence of surfactant, such as surfactant-free Superactive uniform Aldehyde/Sulfate Latexes (Interfacial Dynamics Corp., Portland, Oreg.). Detectable particles can be additionally labelled to enhance detection: for example, a liposome or a latex bead can be dyed or can be made to incorporate fluorochromes.
The type of label for the analyte will vary, depending on the analyte, the membrane, method of detection used, the results sought, and other factors. If the particles themselves are not transparent (i.e., if the particles can be detected using an optical sensor), it may not be necessary to label the particles.
To conduct the assays of the invention, a clearing agent is used. The clearing agent is an agent that causes the membrane to become transparent; that does not dissolve the analyte or interfere with any label used; and that does not significantly displace the analyte. The term, "transparent," as used herein, indicates that light passes through the membrane with little or no scattering caused by the substance of the membrane.
In one embodiment, the clearing agent is within the membrane itself. For example, the clearing agent can be a solid substance that is incorporated into the membrane. For example, if the membrane is nitrocellulose, the clearing agent can be polyethylene glycol, preferably polyethylene glycol having a molecular weight of 1,000. The clearing agent is activated at an appropriate time using an appropriate means for activation. For example, if the clearing agent is a solid agent that liquifies at a certain temperature, and that clears the membrane when it is liquified, the clearing agent can be activated by heating the membrane containing the clearing agent to an appropriate temperature. When the clearing agent liquifies, it clears the membrane.
In another embodiment, the clearing agent is applied onto the membrane to be assayed for the quantity of analyte. The clearing agent is applied to the membrane using standard techniques known to those of ordinary skill in the art. For example, clearing agent can be coated, sprayed, dropped, wicked, sprinkled, or blotted into the membrane. Alternatively, the membrane can be briefly immersed in the clearing agent. In a preferred embodiment, the clearing agent is a liquid, because of the ease of application of a liquid to the membrane.
In one embodiment, the clearing agent is an agent that, when it is a liquid, has approximately the same refractive index as the membrane. The intensity of scattered light depends on the square of the difference in refractive index between the scattering object and the medium in which it is immersed. Therefore, an agent having approximately the same refractive index as the membrane will eliminate interference. For example, the refractive index of nitrocellulose is nD =1.538 (the subscript refers to the sodium D line, the wavelength of which is 589 nm, which is a common wavelength at which to measure the refractive index). Therefore, a clearing agent for nitrocellulose has a refractive index between approximately 1.530-1.545, and preferably between approximately 1.535-1.540, and more preferably approximately 1.538. Representative clearing agents for nitrocellulose include polyvinylpyrrolidone (nD =1.51-1.54, depending on molecular weight); polyethyleneimine (PEI; nD =1.52-1.53, depending on molecular weight) and benzyl alcohol (nD =1.538). In a preferred embodiment, PEI having a molecular weight of approximately 700 is used as a clearing agent for a nitrocellulose membrane. Other clearing agents include mixtures of water and PEI for glass fiber membranes, the exact composition determined to be that which matches the index of refraction of the glass (nD =1.46-1.52, depending on type). If the clearing agent is an agent that has approximately the same refractive index as the membrane, and if the means for detection of the analyte is light scattering, then any label used for the analyte should have a refractive index that is different from that of the clearing agent and the membrane. If a different label is used (such as fluorescence), than it is not necessary for the clearing agent to have a refractive index different from that of the label.
In a second embodiment of the invention, the clearing agent is a dissolving agent. A "dissolving agent," as described herein, is an agent that dissolves the membrane into a clear substance. The dissolving agent is viscous enough to dissolve the membrane, without significantly displacing the analyte from the position it held prior to application or activation of the dissolving agent. For example, nitrocellulose is dissolved by low molecular weight polymers such as polyethylene glycols (PEGs). A single polymer, or a mixture of different polymers, can be used as the clearing agent.
The membrane is maintained under conditions sufficient to allow clearing of the membrane. The term, "clearing" of the membrane, indicates that the membrane becomes transparent. Clearing of the membrane, as described herein, also refers to dissolving the membrane to a clear (transparent) substance.
For example, if the clearing agent is an agent within the membrane or applied to the membrane that, when liquefied, clears the membrane, the membrane is maintained at an appropriate temperature to liquefy the clearing agent. In another example, enough time should be allowed to elapse to allow clearing to proceed. The clearing time varies from seconds to hours, depending on the viscosity of the agent and the membrane characteristics. Some agents act effectively instantaneously upon application or activation; others require time to clear the membrane.
Following clearing of the membrane, the amount of the analyte can be quantified by an appropriate sensor that detects electromagnetic radiation, such as an optical sensor. For example, if the analyte is labelled with a fluorescent label, the amount of analyte can be quantified by measuring fluorescence intensity. The quantity of analyte can be calculated based on the strength of the fluorescence signal. Alternatively, if the analyte is detectable without a label, or if the analyte is labelled using a detectable particle, the amount of analyte can be quantified by measuring the light scattering at one or more angles from the incident light direction
A representative optical sensor, which could be used for a wide range of different types of membranes and analytes, could include the following: a filter or other means to select an emission wavelength for fluorescence measurements; a single or multichannel photodetector device to measure light intensity; a light source for excitation of fluorescence or measurement of reflectance; a collimated light source for forward light scattering measurement; and/or a diffuse light source for transmittance measurement of concentrated analyte. The light source and/or the filter could be selected for the appropriate mode of measurement desired.
More than one type of analyte can be quantified at the same time: for example, analytes having different fluorescent labels can be detected by measuring the fluorescent intensity of each label. Alternatively, one analyte can be quantified by fluorescence, and another by measuring light scattering. A control analyte can also be used and quantified.
The methods of the invention permit highly sensitive detection and quantification of analyte in a membrane. The methods are particularly useful for quantitative immunochromatographic assays to assess the quantity of analytes in a fluid sample, using a Rapid Antigen Measurement Platform (RAMP™) apparatus. In such a quantitative immunochromatographic assay, a RAMP™ apparatus is used. In one embodiment, the apparatus includes a membrane strip made of cellulose nitrate; the membrane strip has an application point, a detection zone, and a contact region between the application point and the detection zone. Imbedded in the contact region is a population of particles, such as colloidal metal particles, organic molecules, liposomes, or organic polymer latex particles, coated with an antibody to the analyte of interest. A detection reagent, such as antibody to the analyte of interest, or the analyte of interest itself, is immobilized in the detection zone. A fluid sample to be assayed for the analyte of interest is applied to the application point, and the apparatus is maintained under conditions which allow capillary action of fluid to transport analyte of interest through the membrane strip to the contact region, so that when analyte reaches the contact region, it binds to the antibody-coated particles. The antibody-coated particles are mobilized by the fluid and move by capillary action through the membrane to the detection zone. In the detection zone, the detection reagent interacts with the antibody-coated particles, leading to arrest of analyte-bound antibody-coated particles in the detection zone. The amount of analyte-bound antibody-coated particles in the detection zone is quantified. The amount of analyte in the fluid sample can be calculated, based on amount of analyte-bound antibody-coated particles in the detection zone. More detailed teachings concerning quantitative immunochromatographic assays are described in U.S. patent application Ser. No. 08/625,048, entitled "Quantitative Immunochromatographic Assay", filed on Mar. 29, 1996, now U.S. Pat. No. 5,753,517 the entire teachings of which are incorporated herein by reference. The accuracy and sensitivity of this quantitative immunochromatographic assay is greatly enhanced by the methods described herein. After arrest of analyte-bound antibody-coated particles in the detection zone of the membrane, the clearing agent is applied to the RAMP™ apparatus membrane, and apparatus is maintained under conditions sufficient to allow clearing of the membrane. Quantification of the analyte-bound antibody-coated particles in the detection zone is enhanced approximately 20-fold by clearing of the membrane.
The invention is further illustrated by the following Example.
A video image analysis system utilizing a DVC 10 bit digital CCD camera mounted on a Wild APOZOOM lens system, an Imaging Technology IC-PCI-2.0 frame grapper and OPTIMAS 5.2 image analysis software on a 120 MHZ Pentium PC was used to quantitate the signal produced by a population of latex particles imbedded in a Sartorius NC 5 nitrocellulose membrane having a transparent Mylar™ backing. Samples of latex particles, stained navy blue and having a diameter of 0.53 μm, were coated with bovine serum albumin (BSA) to a surface concentration of approximately 3×10-4 mg/cm2 and allowed to travel approximately three cm by capillary action in 0.05 M tris-HCl buffer to a detection zone of the nitrocellulose membrane. The detection zone (the area of the membrane which was analyzed to quantify the analyte) had been prepared by applying 3×5 μl of a polyclonal antibody preparation of rabbit anti-BSA IgG to the membrane, blocking with it polyvinyl alcohol (MW 15,000) for approximately four hours, then washing three times with distilled water. The latex concentration was varied from 1.6×10-5 g/ml to 1.3×10-3 g/ml, and 5 μl of latex suspension was applied to the membrane at the opposite end from the detection zone. No latex was detected beyond the detection zone in any membrane upon completion of migration in the membrane, thus implying that essentially all of the latex had been arrested in the detection zone. Duplicate membrane strips, each pair having a different concentration of latex particles, were prepared.
One of each set was imaged with transmitted light without the use of a clearing agent, while the second was treated with a drop of PEG 600 over the detection zone before analysis. For the untreated membranes, the analysis was performed with the video camera on the vertical axis above the membrane and the light supplied by a diffuse source below the membrane and aimed directly at the camera. The focussed image of the detection zone produced by the transmitted light was then analyzed.
For the membranes treated with a clearing agent, the analysis was performed by recording and analyzing the image obtained when the membrane was illuminated from below with a collimated beam oriented at an angle of about 30 degrees from the vertical, with the camera again located above the sample on the vertical axis. In this case the background in the focussed image of the detection zone appeared dark, with the arrested latex forming a bright band as a result of the forward scattered light collected by the camera.
In each case, the image was optimized by background subtraction, and the final image was integrated to provide the integrated pixel intensity representing the signal from the latex particles.
Results are shown in the FIGURE, where the relative integrated signal intensity is plotted as a function of the relative number of latex particles applied to each membrane. The results demonstrate a large increase in signal provided by the cleared membrane relative to the uncleared membrane.
Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described specifically herein. Such equivalents are intended to be encompassed in the scope of the following claims.
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Owner name: BRITISH COLUMBIA, UNIVERSITY OF, CANADA
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:BROOKS, DONALD ELLIOTT;REEL/FRAME:008286/0213
Effective date: 19961219
|14 Jul 2004
|Maintenance fee reminder mailed
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|Expired due to failure to pay maintenance fee
Effective date: 20041226


text:
Police: Auburn flasher also seen at middle school in Marcellus
Tue, 23 Nov 2010 20:45:42 GMT —
Police say a man who flashed high school students in Auburn was also caught on camera at a middle school in Marcellus.
Police say a suspect unlawfully entered Auburn High School on November 4th and exposed himself to a student. Now investigators say they've received information from the Marcellus Police Department that the same suspect also unlawfully entered Driver Middle School at approximately 5:30pm on the same day. At Driver, the suspect was confronted by maintenance staff and left the area. The Onondaga County Sheriff's Department was called to the school, but did not find the suspect.
Surveillance images from Driver show the suspect in both a dark gray hooded sweatshirt, and the lighter colored sweatshirt underneath. The lighter colored sweatshirt has a unique design on the front. In most of the images, the suspect's face is covered.
The suspect left Auburn high School in a dark colored, four-door car.
Auburn Police officers say they are working on a few leads. They're hoping this extra information will help catch the suspect. If you know anything regarding the investigation or the suspect's identity there are several numbers you can call:
Marcellus Police Department: 673-2079Marcellus School District: 673-6200Auburn Police Department: 253-3231
Click here to see video clips of the suspect inside Driver Middle School. Scroll down to the bottom of the list.


text:
Technology Poems. Below are examples of poems about technology. This list of poetry about technology is made of PoetrySoup member poems. PoetrySoup is a great resource for examples of technology poems or a list of technology poetry. These examples show what technology poems look like. There is also a link below to the definition of technology and a page where you can discuss these types of poems.
The technology poem sub-topics listed below include many popular topics of technologypoetry.
The poem(s) are below...
machinery, mechanics, automation, computers, robotics, telecommunications, mechanization, applied science, electronic components, hi tech, high tech, industrial science, scientific know-how, scientific knowledge, technical knowledge,
Note: The forms for these new poems were selected by the poet. Often poems are assigned the wrong form. Please confirm the accuracy of the poetic form before referencing the poem.
Don't see what poems you want? Try our Advanced Poem Search for more results!


text:
Sales & Marketing Intern
Shaastr- Where Gurus Go OnlineMumbai
Internship DescriptionWe are a Powai based startup founded by IIT Bombay almunus. Disrupting the education space is our passion. Learning and adapting with the changes across mobility and education technology is in our blood. We are Shaastr - an IITian online test prep platform. Come, be a part of our successful startup journey with our test prep platform that helps small teaching institutions experience the power of the internet! Internship Description: 1. Getting in touch with coaching classes of as little as 30 students and convincing them to try Shaastr out for a 2 month free trial period. 2. Conducting the product demo for the client. 3. Getting the client to send us their email ids and question banks once convinced. 4. Updating your Google sheet everyday on the status of your progress.
About Company
Shaastr is an effortless way to take your test prep online, a new age platform where
Gurus go online- A startup byIITians.
Whether you're a teacher, home tutor or freelance trainer, you can seamlessly conduct your tests with our one step solution designed to simplify and empower at the same time.
Come, build your own Shaastr and upgrade your teaching experience today!


text:
Spellbinders Paper Arts contest on this blog has ended.
Don't forget that you still have time to enter
at the blogs listed below. Thank you!
• Be eligible to win the Grand Prize by entering each day (see schedule below)!
• One entry per person, per blog please, on the designated days!
• Each blog will accept entries for 48 hours!
• You have from 12:01 a.m. Eastern Standard Time July 9, 2011 to 12:01 a.m. July 11, 2011 enter my contest. See the Official Rules here.
• Today you can enter my contest and Gloria Stengel's contest.
I just knew when I saw these fabulous box templates, the possibilities were endless. Wedding favors, birthday gifts, party favors, ADVENT calendars!
Here are the dies I introduced:
LF-010 Square Bracket Edge Box
Bottom: 5 1/2 x 5 1/2"
Top: 4 x 4"
Flowers: 1"
S5-067 Anemone Flower Topper
Center: 1 3/8"
1: 2 3/8"
2: 2 3/4"
3: 3 1/4"
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text:
By Bonnie Coblentz
MSU Ag Communications
STARKVILLE, Miss. — Some of Mississippi's future medical professionals demonstrated their dedication by taking college-level classes the summer before their senior year in high school.
This year, 23 academically gifted high school students participated in the five-week Rural Medical Scholars program at Mississippi State University. Since the program began in 1998, 317 students have participated, experiencing college life and shadowing doctors and other medical professionals for an on-the-job view of their professional lives.
Bonnie Carew, MSU Extension Service rural health program leader, coordinates the unique and challenging program.
"As a whole, Mississippi lacks an adequate number of medical professionals to serve the needs of the population," Carew said. "This program was started to encourage the best and brightest of our high school students to consider the field of medicine for a career. We designed this program to give them valuable information to help as they make decisions for their future."
The program's primary purpose is to create more physicians for Mississippi. It houses students in residence halls, enrolls them in two college classes and makes them responsible for time management.
This year's students took freshman biology and sociology. They were in class four hours every morning and had lab four afternoons a week. On Tuesday afternoons, the students shadowed practicing physicians as they met with patients and performed their duties.
"I felt like it would really help me decide if I wanted to be a doctor," Jason Carter of Horn Lake said of why he chose to participate in the program.
Sabrina Micha of Starkville was considering both engineering and pediatrics as possible careers when she signed up for the program.
"I figured I'd get a firsthand experience of what it would be like in a health-care job," Micha said. "After getting to shadow a pediatrician, I decided I want to do pediatrics."
In addition to the classes and the on-the-job experience, scholars also spent a day in Jackson at the University of Mississippi Medical Center. They got to talk to the dean of admissions and speak with current residents at the school.
"The dean told us how to get into medical school," said Elizabeth Tedford of Clarksdale. "I probably would have failed otherwise if I had tried to apply on my own."
The Rural Medical Scholars program is operated by the MSU Extension Service. Funding for the program comes primarily from the Extension Service with assistance from the State Office of Rural Health at the Mississippi Department of Health.
On July 7, Rural Medical Scholars will graduate its 15th class of students. Overall, 70 percent of previous classes pursued health-related careers. Since the program began, 34 scholars went to medical school and 24 are practicing physicians. Fourteen of these physicians chose to stay and practice in Mississippi.
Dr. Douglas "Deke" Barron is a former Rural Medical Scholar who is now in family medicine residency at the North Mississippi Medical Center in Tupelo. The Hernando native credits the program with giving him an early idea of what college life was like and allowing him to see that he really liked medicine as a career.
"I would say Rural Medical Scholars' exposure to the medical setting via the shadowing experiences and the trip to UMMC were what cemented my decision that I wanted to pursue a career in medicine," Barron said. "It also showed me that in Mississippi in particular, a career in medicine would be of great use to both myself and the state."


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|low graphics version | feedback | help
|You are in: World: Europe
Wednesday, 13 September, 2000, 15:47 GMT 16:47 UK
Europe fuel protests escalate
Belgian lorry drivers have stepped up their protests against high fuel prices by blockading the port of Antwerp, the second largest in Europe.
All major roads to the port are reported blocked and dock officials are warning that if the blockade lasts for a couple of days, the effects could be "catastrophic".
Roadblocks have been set up on motorways across the country, and the streets of the Belgian capital, Brussels, remained virtually empty on Wednesday as truckers took their protests there into a fourth day.
The blockades, which have now spread to several other European countries, follow the action of French road hauliers who last week forced their government to reduce fuel taxes after a week of action.
A European Union delegation is due to meet foreign ministers from six oil producing countries on Wednesday to discuss ways of resolving the crisis.
In the Netherlands, truck drivers are continuing to block several major routes following the rejection of fuel price cuts by the Dutch Finance Minister Gerrit Zalm.
And German truck drivers and farmers are staging fresh protests around Munich.
Other action is beginning to extend as far as Poland, where truck drivers and fishermen have warned they intend to block oil refineries and ports.
Drivers angry
Despite Germany having some of the lowest fuel prices in Europe, drivers are angry at Chancellor Gerhard Schroeder's policy of additional fuel "ecology taxes".
In Belgium, talks are continuing between union leaders and the government amid signs the two sides may be nearing an agreement, which could lead to the lifting of the blockade.
However, the government has so far refused to consider lowering high fuel prices, the trucker's central demand, and is instead offering a series of measures designed to soften their impact.
Belgian protesters say they will keep their barricades in place for as long as it takes to force their government to climb down.
"One week, two weeks, three weeks - no problem," said one protesting driver.
The Belgian Government is due to decide what to do about the protests at a meeting on Wednesday.
The Commission fears the blockades are restricting the movement of goods, and that German truckers' and farmers' threats to step up their action could worsen the situation.
Protests spread
The protests also seem set to spread further, with truckers and farmers in Spain and Ireland threatening similar action over high fuel prices.
France is also calling for a meeting of European transport ministers next week to discuss the possibility of harmonising fuel prices and taxes.
"People must understand that these problems are complicated, and have to be solved together," said Prime Minister Lionel Jospin.
French authorities are also working on a comprehensive energy saving plans in response to the surge in world oil prices.
See also:
13 Sep 00 | Europe
Bruxelloise find their feet
13 Sep 00 | Europe
German fuel row turns political
12 Sep 00 | Europe
In pictures: Going nowhere fast
13 Sep 00 | Europe
European press review
11 Sep 00 | Europe
France looks for energy savings
11 Sep 00 | UK
Fuel price cut ruled out
05 Sep 00 | Europe
Europe faces fuel dilemma
11 Sep 00 | Business
Oil price bounces back
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text: Birded Lake Puckaway by boat this morning. Only unusual gull amongst the 500 or so ring-billed gulls was a juvenile Greater Black-backed Gull. Something flushed the whole gull flock, but could not see what it was although I saw a suspected Peregrine later in the morning. Was hoping for a Jaeger, but no dice. Other birds included: 1 Common Tern 1 Forster's Tern 2 dozen Tree Swallows 1,000 or more Double-crested Cormorants (scanned for Neotropics) 3 American Golden Plovers 1 Spotted Sandpiper 1 possible Peregrine 3 dozen Lesser Scaup Daryl & Sherry Christensen www.darylchristensen.com "There is symbolic as well as actual beauty in the migration of birds. There is something infinitely healing in the repeated refrains of nature - the assurance that dawn comes after night, and spring after the winter." -Rachel Carson #################### You received this email because you are subscribed to the Wisconsin Birding Network (Wisbirdn). To UNSUBSCRIBE or SUBSCRIBE, use the Wisbirdn web interface at: http://www.freelists.org/list/wisbirdn. To set DIGEST or VACATION modes, use the Wisbirdn web interface at: http://www.freelists.org/list/wisbirdn. Visit Wisbirdn ARCHIVES at: http://www.freelists.org/archives/wisbirdn.


text: Paul Sturcken was a wonderful vendor to work with. He was extremely personable and professional. Loved the final video. Everyone commented on how much fun he was during the reception. If you're truly looking for a pro. You will not be disappointed. I'm telling all my engaged friends to call Paul.


text:
Unfortunately, this job is expired as of 1/26/2016.
To continue in your search through the appropriate job categories, click either Administrative Staff, Higher Education Executives, Faculty, Post-Doc and Graduate Assistants, . You can also utilize "guess-free" keyword search tools with up to eight pre-defined criteria. Search for and Apply to academic postings directly from the site. Post your background either confidentially or overtly. Stand out and be discovered!
UMass Lowell
Assistant/Associate/Full Professor - Marketing
Marketing, Entrepreneurship & Innovation - Manning School of Business
The Manning School of Business (MSB) at the University of Massachusetts Lowell is seeking tenure-track Assistant, Associate, and Full professors in Marketing. The preferred fields of specialization are: new product development, global marketing, marketing analytics, sustainability marketing, and social media marketing. Also of interest is some combination of marketing research, b2b marketing, e-marketing, services marketing, and entrepreneurship. Faculty members earn teaching load reductions for the publication of high quality research, typically for 2/2 per academic year. Ample opportunities are available to become involved in research and instructional activities and develop productive partnerships with other units of the University, industry and with colleagues from other institutions and countries. Classes are taught on-campus, online and, increasingly, abroad.
Located 30 miles northwest of Boston, UMass Lowell has over 17,000 students and is one of the largest public universities in Massachusetts. The MSB has over 2,500 undergraduate and graduate students and is accredited by the AACSB International. The School strongly supports the faculty to further enhance their research productivity and build program offerings at all degree levels. The successful applicant will have the opportunity to participate in (1) the growth and delivery of new academic programs such as the Ph.D. program with specializations in international business, leadership and organizational behavior, entrepreneurship, finance, accounting and MIS, (2) the growth of our Master of Business Administration, Master of Science in Innovation and Technological Entrepreneurship, and undergraduate Marketing and Entrepreneurship programs, and (3) ongoing initiatives undertaken in research, instruction and outreach.
Preferred Qualifications:
To apply, please submit a resume, cover letter, sample of scholarly work, evidence of teaching and the names and contact information for three professional references to jobs.uml.edu/applicants/Central?quickFind=54517. Review of applications will begin August 1 and will continue until the position is filled. However, the position may close when an adequate number of qualified applications is received.
Names and contact information for three references should include position title, employing organization, mailing and email addresses, ***Do not send recommendation letters
The University of Massachusetts Lowell is an Equal Opportunity/Affirmative Action, Title IX employer. All qualified applicants will receive consideration for employment without regard to race, sex, color, religion, national origin, ancestry, age over 40, protected veteran status, disability, sexual orientation, gender identity/expression, marital status, or other protected class.
|Employment Type:
|Degree Required:
|See Job Description
|Level of Job:
|Associate Professor
|Type of Faculty:
|Tenure Track
|Not Specified
|Type of School:
|4 - Year / Masters Institution
|Application Requirements:
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text:
Prevention and Early Detection is out motto. Go through each of the link and understand Kidney Disease.
- Prevention
- KIdney Patient Care
- Promotion of Organ donation
In case you do not want to download and print send your postal address we will send you hardcopy.
Email us on email@example.com
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For more information about these and other details. Please email us at email@example.com
Every single step towards us will help someone. You can decide the mode.


text:
What's more clich than surfing? The term "surfer" no longer conjures up a single image of the sun-bleached "dude" of yesteryear. Today, a whole new slew of surf characters clogs the lineup. They often travel in packs, sticking closely to their kin, but the daily invasion of your local break wouldn't be complete without this distinct cast of characters:
Sponsor Monster
You know him, he's the kid who gets a few T-shirts, maybe a pair of trunks and a rashguard from his buddy who reps for a company, then proceeds to put an 8″x10″ sticker on the nose of his board. He's the kid who's got stickers from a random surf shop, a no-name start-up surf company, and a small-town restaurant chain plastered all over his stick. He may not need to claim any salary from his sponsors on his IRS-1040, but don't worry, heads turn as he walks down the beach, and that's all that matters.
He may still live with his parents and work construction, but he's got his lifted '97 Tacoma almost paid for and his girlfriend's dad said that he might need someone to start doing their yard work starting next month. After his brief stint on the 'QS, he spent several summers hosting surf camps, until he slowly lost his name recognition and the next ex-pro stepped up (or, uh, down) and took his place. He drinks a few beers with buddies from time to time (that is, morning time to night time), and still loves a good party.
Wait, there are surfers who aren't "artists"? Ever since "art" was redefined to include any half-assed scribble or paint splatter, the surfer/artist category has increased tenfold. Not to be confused with homeless people, Euros, or the color blind, these surfers generally dress in a messy hodge-podge of colors, tight pants, and the occasional odd hat. They make their living by selling their artwork to friends and at local galleries, where they are able to ride the wave of surf hype to the peak of exorbitance, getting far more money for it than they would ever be able to get in the outside world.
This guy has the brand new Ford off-road vehicle with roof racks, a tow hitch, the convertible tent pop-top, and a whopping 5 highway miles per gallon. He just got two new stand-up paddle boards to add to his twenty-board quiver consisting largely of 9'6"-10'4" longboards, all in mint condition and barely ridden. He just got brand new booties and 2008 Rip Curl suits in 4/3, 3/2 and a short-arm-spring for the summer. He's gone on boat trips to the Maldives, where he and his five closest bootie-and-springsuit-wearing friends spend $7000 each for 5 days worth of empty surf. Screw this guy.
God Squad
God has a special place in his heart for surfers who choose Him over Vodka-Red Bull and sex. These saintly surfers straddle the divide between selflessness and self-promotion with an ease that could only be explained by the influence of the divine. In their synchronized attempt to feed the starving children and make cash surfing, they often end up accomplishing neither. But, you gotta love them—every Sunday morning the Church bells ring and they clear the water just for you.
Surfing is all about being one with the ocean, gliding like a dolphin, being in touch with the Earth and letting mother nature embrace you in her loving barrel-y arms, or so the transcendentals would proclaim. Usually amassed in this crowd are also the granola-munchers, hopped up on wheat grass and flax seeds, doing yoga while simultaneously saving the whales and most of the trees, all while singing a lovely acoustic song about it. They generally mind their own business, remaining slightly detached from the surf scene. You'll usually spot them as they pull out of the parking lot in their Biodeisel vans, leaving you in their hazy wake of vegetable oil and marijuana smoke.
Of course none of us fall into any of these stereotypes. We aren't in any designated group. We don't adhere to labels. We are individuals. We are distinct. We create our own identities. Man, how clich.


text:
Vodafone's pay as you go Facebook phone has gone onsale in the UK. The Vodafone 555 is unusual in being a keyboard-centric handset, bucking the current trend for touchscreen devices.
The £62.50 handset has a 2.4in QVGA screen, a 2Mp camera with a flash and can also capture video. There's an SD card slot (max capacity 16GB) for photo and music storage, an FM radio and a media player. Connections run to Bluetooth 2.1 and Wi-Fi, so you'll need to log on at a wireless hotspot or use your home Wi-Fi connection whenever you want to update your Facebook or Twitter status. An integrated updates screen lets users keep abreast of friends' statuses, photos and contacts.
The 100g Vodafone 555 handset runs the Opera Mini browser and features staples such email, as a stopwatch and calculator but no fancy extras such as real-time weather updates. Vodafone cites a seven-hour battery life with a standby time of more than 600 hours. The phone is quad-band and, in addition to Wi-Fi and Bluetooth, offers GPRS and Edge connectivity.


text:
Preah Vihear re-visited
A new book entitled Preah Vihear - A guide to the Thai-Cambodian conflict and its solutions will be released at the end of September with a book launch at the FCC of Bangkok on 30 September. The Thai-Cambodian relationship in recent years has been shaped and reshaped by domestic factors both in Thailand and Cambodia and with overlapping territorial claims in the areas surrounding the Preah Vihear temple, or known in Thai as Phra Wihan, this has led to a series of clashes between the two countries' armies, from 2008 to 2011. The border dispute has re-opened the question of ownership of the Temple of Preah Vihear which compelled Cambodia to call on the International Court of Justice (ICJ) to reinterpret the ruling of 1962. The authors of the book are Cambodian Ambassador Pou Sothirak and Thai scholars Ajarn Charnvit Kasetsiri and Dr. Pavin Chachavalpongpun.
Labels: Preah Vihear


text:
Hello all,
I am wondering if anyone knows a good housekeeping gene to use in western blotting from whole cell lysates that is a very low molecular weight? I cannot use actin, as I normally would, because my protein of interest is 45 kD. Preferably, (since I ran out the dye and the lowest band from my ladder is 20 kD) I am interested in something between 20 and 25 kD, give or take a few kD.
Thank you in advance,
Submit your paper to J Biol Methods today!
Low Molecular Weight Housekeeping Gene for Loading Control?
Started by Lauralia34, Oct 19 2011 11:42 AMwestern blot loading control low mol weight housekeeping gene
2 replies to this topic
Posted 19 October 2011 - 11:42 AM
Posted 19 October 2011 - 11:43 AM
I should add that my gel has a number of (as of yet) unspecified bands, one of which shows up around 37 kD, so I'm not sure that even GAPDH would work.
Posted 26 October 2011 - 05:40 AM
Tubulin has a band of aprox 50 KDa, maybe you can use it, or GAPDH if you change the secondary antibody (if your first Ab is made in rabbit, you can buy a GAPDH Ab in mouse)
Hope it helps you
Hope it helps you


text:
Tehran: Iran's supreme leader said today the United States was seeking to influence next month's elections in the Islamic republic but said such efforts would receive a "punch in the mouth".
Ayatollah Ali Khamenei's comments related to parliamentary polls and a ballot for the Assembly of Experts, a powerful committee of clerics who will pick the country's next supreme leader when the incumbent, who is 76, dies. Both elections take place on February 26.
Political moderates and reformists are seeking to wrest back control of the currently conservative-dominated 290 member parliament on the back of President Hassan Rouhani's nuclear deal with world powers last year.
The Assembly of Experts is also heavily made up of conservatives.
Some 12,000 candidates have registered for the parliamentary election but many are expected to be excluded during vetting procedures carried out by another top Iranian committee, the Guardian Council.
Khamenei told a meeting of prayer leaders that attempts by "enemies" to distance Iran from its revolutionary mandate — the Islamic republic was formed in 1979 after the overthrow of a US-backed shah — would fail.
"Americans have set their eyes covetously on elections but the great and vigilant nation of Iran will act contrary to the enemies' will, whether be it in elections or on other issues, and as before will punch them in the mouth," he said.
The supreme leader, whose authority outweighs all politicians including Rouhani, has warned in recent months that July's nuclear deal — which is yet to be implemented — would be followed by US attempts to "infiltrate" Iran.
Reiterating such sentiments today, he said the US goal was to bring the Islamic republic closer to "their own goals rather than Iran's goals of the revolution".
"Those who have access to information know what traps have been laid or are being laid for the country in order (for them) to infiltrate the nation's will and decisions," Khamenei said.
"We must all be vigilant with regards to infiltration," he warned, comparing any such person who managed to find their way into parliament or the Assembly of Experts to "termites who would gnaw and weaken (Iran's) foundations from within.


text:
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Classic Toy Rubber Duckies
Is their yellow Rubber Duckie feeling a little lonely? Bring him home some friends. These Rubber Duckies are made with fun costumes for an updated version to the classic bath toy. Great party favors. 12 count.


text:
President Bush Still Rules After Jan. 20?
He may be a lame-duck president, but George W. Bush is determined to keep quacking away federal regulations before his time is up. Since many of Bush's plans contradict Barack Obama's agenda, why would he waste time issuing rules if they would soon be undone? The answer? It could take years for Obama to rescind some of these rules.
Nonprofit ProPublica has compiled a list of the Bush administration's last-minute rules and regulations. According to the list, some of the proposed changes would let federally funded institutions turn down abortions for moral or religious reasons; give greater surveillance authority to law enforcement; lease public lands for oil; allow construction projects with less concern for protected species; and subject HIV/AIDS prevention groups to new qualifying rules.
Obama's transition team has a close eye on these midnight regulations, but protracted efforts and court battles aimed to undo the rules surely lie ahead.


text:
SALT LAKE CITY, UTAH- The 2010-11 Utah ski and snowboard season got off to a sprinting start with over 96 inches of snow falling in the month of, what shall now be called, SNOWVEMBER. The past month's snow has brought Utah's season snow total to 138 inches with base depths as high as 67 inches.
National Weather Service Hydrologist Brian McInerney reports that as of November 29, 2010 watersheds from Little Cottonwood Canyon north to City Creek Canyon are an average of 133 percent of normal and the Snowbird watershed is 191 percent of normal (graph attached). McInerney also noted, "When we start early, we tend to get better all year long."
In addition to incredible conditions, Utah ski resorts are offering a plethora of deals through Ski Utah's White Sale including:
-Park City Lodging, Inc.- Receive 15 percent off your ski vacation at Deer Valley, Park City or Canyons when you book with Park City Lodging, Inc. by December 5, 2010. No blackout dates. Minimum nights do apply and other restrictions may also apply.
– Park City Marriott Snow Dance Celebration rates starting at $169- Book a weekday stay by December 24, 2010 and receive a rate of $169. Certain restrictions apply. Offer good for Sunday-Wednesday night stays only. Rate is per night and does not include tax. Valid for stays December 1, 2010- April 10, 2011.
-Snowbasin/Powder Mountain Package at Lakeside Resort Properties – $140 per person per night. Includes complimentary shuttle to Snowbasin and Powder Mountain, lift tickets, private hot tubs and much more.
-Snowbird Early Bird Stay and Ski Package from $99*- Package includes a minimum of three nights lodging and a three-day Snowbird only Tram and Chair pass per adult. Additional nights and lift passes can be purchased. *Price is per night, per adult based on two staying on the package. Children 12 and under ski free for every night of stay (kids 7 – 12 will get a voucher at check in). Valid Now through December 22, 2010. Rates will vary based on accommodations and dates selected.
For more information on these deals or to view a complete list of White Sale offers visit: http://www.skiutah.com/whitesale/.


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