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Hello Middleton family! Welcome to the dank, foetid world of celebritidom! In this new realm which you now find yourselves in, you'll find that people have no qualms about digging up old photos of you in states of undress or looking drunk, or indeed, sticking SLRs up your skirt to snap your gussets!
Hurray for that.
And with that, Kate Middleton, her mother Carol, and sister Pippa (or, as you'll know her, 'The One With The Nice Arse') have filed an official complaint against newspapers who published pictures of them in their bikinis.
Now, if you haven't seen the pictures in question, feel free to check for them online because, despite this complaint, they're still absolutely everywhere.
Are you back with us now? Cleaned your hairy little guts of sputum? Good.
These photographs are five-years-old photos and were taken when the Middletons holidayed on a yacht in Ibiza. That's right – a yacht. Presumably, it was a 'commoners' yacht.
And now, despite the fact these photos were published ages ago, now that the Middleton family are down with Prince William & Co, they've lodged a complaint with the Press Complaints Commission because they think they're privacy has been breached.
Not unlike the wedding day which wasn't a mass of invaded privacy.
A press watchdog spokesman said, in a voice as high as a green fly with his balls in a vice, said:
"I can confirm that the PCC has now received complaints from representatives of the Middleton family concerning photographs published in the News of the World, Daily Mail, Mail on Sunday and Daily Mirror. We will now consider those complaints."
Clarence House don't want to comment on the whole thing it seems.
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The Palestinian Uprising Was Written on Hollywood's Red Carpet
While neither local documentary won an Oscar this year, the hoopla surrounding the films helped wake Israelis up to the ugly reality of the occupation.
There is nothing further from the reality in the West Bank than the Academy Awards ceremony in Los Angeles. Although they are a 16-hour flight apart, the...
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Quote of the Day
"Why are you keeping that a secret from your audience? In the 21st century we have this thing called the Internet. People in Swaziland know a no-hitter is going on."-- Dodgers radio annoucer Charley Steiner, on the Angels' television crew not mentioning Jered Weaver was in the middle of a no-hitter during Wednesday's game against the Twins. (L.A. TIMES, 5/4)
Top Stories
Numbers Never Lie*
The Red Sox, who have sold out 723 consecutive home games through Wednesday, "embrace an alternate definition that permits them to declare a game sold out even if hundreds of tickets go unsold but others are distributed for free," according to a front-page piece by Hohler & Lakso of the BOSTON GLOBE. Red Sox Exec VP & COO Sam Kennedy said, "I can understand the confusion. But we operate by a definition that is commonly practiced throughout Major League Baseball and professional sports." Hohler & Lakso note the Red Sox count the "total number of tickets they distribute, including an average of 800 complimentary tickets each game to charities and others, as the basis for a sellout." They also count "standing room tickets toward th ...
Big Blows
Will Seau's death, concussion suits force NFL to take a harder look at safety in the game?
Sky Walker
Sky facing more competition for EPL domestic TV rights.
Taking The Air Out Of The Ball
College basketball loses perhaps its highest-profile non-conference rivalry in Indiana-Kentucky.
Not In My House
Nats hope Take Back The Park promo will mean fewer Phillies fans head to DC this weekend.
Changing The Channel
Cablevision's Q1 profits down 45% due to weak cable earnings.
Prized Fighter
Floyd Mayweather covers the costs, but also reaps the rewards when he headlines a PPV bout.
Spinning Their Wheels?
IndyCar CEO: Edmonton Indy needs a successful event this year to remain on the series schedule.
Safe Bet
Bill Simmons withdraws MVP vote to avoid conflict of interest.


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My Nonprofit Reviews
Review for Animal Services and Assistance Programs Inc, Marengo, IL, USA
ASAP is a wonderful organization that serves both local and national animal rescue organizations, as well as providing many services to local people through their TNR program. They also have a wonderful Guardian Angels program that helps provide medical care to ill or injured animals in shelters, then finds them placement in a no-kill shelter until they are adopted. I've been blessed to get to participate in several of their pet food distributions for animal rescue groups, as well as fostering kittens and cats in need of a temporary home & helping them get adopted. Love this organization, and the great things they do for animal rescue groups!
More Feedback
Would you volunteer for this group again?
For the time you spent, how much of an impact did you feel your work or activity had?
Did the organization use your time wisely?
Very Well
Would you recommend this group to a friend?
When was your last experience with this nonprofit?


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|XP is just a number
Re: CTRL+C Handling in Perl Threadsby zentara (Archbishop)
|on Nov 27, 2012 at 10:46 UTC ( #1005836=note: print w/replies, xml )
|Need Help??
See using the thread->kill() feature on linux. When it comes to threads, you must put the signal handler into the thread code block. The main thread receives the signal first, then internally passes it on to each thread, so each thread must have a handler.
I'm not really a human, but I play one on earth.
Old Perl Programmer Haiku ................... flash japh
In Section Seekers of Perl Wisdom


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CARRABELLE (AP) A corrections officer was fired after zapping children with 50,000 volts of electricity during a "Take Our Daughters and Sons to Work Day" tour, authorities have said.
Sgt. Walter Schmidt says the parents gave him permission to shock the children with a handheld stun device.
Warden Duffie Harrison of the Franklin Correctional Institution said Schmidt zapped "at least two kids."
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With Cancer, You Must Accept the Things You Cannot Change
The second line of the serenity prayer is "to accept the things we cannot change." This sounds really easy but in reality can be quite difficult. A prime example of this is cancer. For those of us who contract cancer, we cannot change that fact. Once cancer is accepted, our attitude toward it changes.


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Day camp services namely, activities in the nature of beach trips, nature walks, swimming trips, picnics and recreational camp services for hotel and resort guests
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Bone remodeling is a localized process, but regulated by systemic signals such as hormones, cytokines, and mechanical loading. The mechanism by which bone cells convert these systemic signals into local signals is not completely understood. It is broadly accepted that the "prestress" in cytoskeleton of cells affects the magnitude of cellular responses to mechanical stimuli. Prestress derives from stiff cytoskeletal proteins and their connections within the cell and from cell contractility upon attaching to matrix. In an in vitro model of three-dimensional gel compaction, the relative cellular prestress levels in the same matrix environment were determined by matrix compaction rate: a greater compaction rate resulted in a higher level of prestress. In the present study, the effects of ATP on the prestress of osteoblasts were studied using mouse MC3T3-E1 cells grown in three-dimensional bioartificial tissues (BATs). ATP (≥100 μM) reduced the compaction rate of BATs in a dose-dependent manner. ADP, 2′-(or 3′)-O-(4-benzoylbenzoyl) ATP, and UTP, but not α,β-methylene ATP, also reduced the compaction rate but to a lesser extent. Pyridoxal-phosphate-6-azophenyl-2′,4′-disulfonic acid tetrasodium did not block the effect of ATP on BAT compaction rate. These results indicate that both P2X and P2Y receptors are involved in ATP-induced reduction of BAT compaction rate. Steady fluid flow and RT-PCR results showed that ATP reduced cell attachment on type I collagen by downregulating the expression of integrin α1. These results suggest a potential role for P2 receptors in matrix remodeling and repair and as a potential drug target in treatment of bone diseases.
- adenosine triphosphate
- three-dimensional culture
- purinoceptor
- osteoblast
- extracellular matrix
the adaptive response of bone cells to mechanical strain is a primary determinant of skeletal architecture and bone mass (14, 16, 45). In vivo mechanical loading induces new bone formation and increases bone mineral density, whereas disuse, immobilization, and weightlessness induce bone loss (16). Although the mechanisms defining how bone cells convert mechanical loading into chemical signals are not completely understood, the prestress in the cytoskeleton (residual stress) of cells is well known to affect their response to mechanical loading (40, 50).
ATP release from mechanically stimulated cells (shear stress, stretch, osmotic swelling, and compression) is exhibited by many cell types (7, 33). Purinoceptor pathways intersect those that regulate mechanical load responses (50). Therefore, extracellular ATP could modulate cellular responses to mechanical loading (42). It was hypothesized that extracellular ATP may act as a regulator and/or transducer of mechanosignal transduction by regulating a cell's set point for prestress. Since bone remodeling is a localized process (3), the local extracellular ATP may play a critical role in bone remodeling by converting the broad, systemic mechanical stimuli into local signals. Extracellular ATP, released from cells by lytic or nonlytic mechanisms, plays a number of diverse roles in vivo and in vitro, such as membrane permeability (27), induction of apoptosis (44), organic anion transport (such as bilirubin) (5), calcium mobilization (12), cell proliferation (38), contractility (34), wound healing (8), excitation of sympathetic neurons (25), inhibition of tumors (28), and bone remodeling (3). ATP interacts with P2 receptors that have been divided into two subfamilies: P2X (ligand-gated cation channels) and P2Y (G protein-coupled receptors) (23). To date, seven P2X and eight P2Y receptors have been cloned and characterized (11, 23). P2X receptors are activated exclusively by adenine nucleotides, whereas P2Y receptors are responsive to adenine (P2Y1, P2Y11, P2Y12, and P2Y13) or uridine nucleotides (P2Y6 and P2Y14) or to both (P2Y2 and P2Y4). P2 receptors are expressed ubiquitously, but specific tissue responses are achieved by cell-specific expression profiles (3). P2 receptors are expressed on osteoblasts, and nucleotides are important local signaling molecules in bone (3, 9). However, few studies have investigated the potential roles of extracellular ATP in the regulation of bone remodeling (26).
As a tissue develops, its cells fabricate an extracellular matrix in a given geometry, according to developmental pathway cues (19). During this process, cells utilize a mechanical sensor(s) and feedback control to establish a set point for a basal prestress at the cell level. This strain set point is determined, in part, by the cell's connectivity to the substratum and internal architecture that balance external and internal forces in a tensegrity structure (40). Cells respond to external tension by adjusting their shape, connections to matrix, and other cells and their prestress. Thus cells develop a prestress level for a given external tension and attempt to modulate their cell-matrix contacts, pseudopod lengths, degree and types of cytoskeletal organization, and modulus of elasticity based on the prestress values (1).
In the present study, mouse osteoblast-like cells were used to investigate the effect of extracellular ATP on bone cell prestress by monitoring the rate of collagen gel compaction. MC3T3-E1 cells were grown in a novel three-dimensional (3D) culture system in which cell-generated force and movement compact the gel matrix through connections between cells and matrix (17). Tension in the matrix increases due to tractional structuring (39). This, in turn, causes cellular prestress levels to increase during the compaction of collagen gels (39). It was believed that increase in cell prestress would make cells more sensitive to mechanical stimulation (6). We have reported that ATP blocked a cellular response to mechanical loading (calcium signaling) (42); therefore, it was hypothesized that high-concentration ATP would reduce cellular prestress and thus reduce collagen gel compaction rate and that P2 receptors play an important role in this process. Since bone repair and remodeling are regulated by both chemical and mechanical stimuli (14, 16, 45), the results in the present study will also be helpful in understanding bone repair and remodeling processes.
All nucleotides except the stable analogs of ATP and ADP were obtained from Amersham Biosciences (Piscataway, NJ). Type II collagenase was purchased from Worthington (Lakewood, NJ). 2′-(or-3′)-O-(4-benzoylbenzoyl)ATP, tris(triethylammonium) salt [BzBzATP or BzATP, a P2X4-preferring agonist (10)] was purchased from Invitrogen (Carlsbad, CA). Adenosine 5′-(3-thiotriphosphate) trilithium salt (ATPγS), adenosine-5′-O-(2-thiodiphosphate) trilithium salt (ADPβS), α,β-methylene adenosine 5′-triphosphate lithium salt (mATP, an agonist for P2X receptors), pyridoxal-phosphate-6-azophenyl-2′,4′-disulfonic acid tetrasodium [PPADS, a P2X-preferring inhibitor (30)], and all other chemicals, unless indicated otherwise, were purchased from Sigma (St. Louis, MO).
Cell culture.
The murine osteoblast-like cell line, MC3T3-E1, was derived from calvariae from newborn mice (41). It is an established cell line, but the cells maintain much of the tightly linked controls between proliferation and differentiation that usually are seen only in primary cells (35). MC3T3-E1 cells were maintained in DMEM medium with 10% bovine calf serum (BCS) and 1% penicillin/streptomycin (Gibco, Grand Island, NY). Human osteoblast-like cells, SaOS-2, were maintained in McCOY's 5A medium containing 10% fetal bovine serum (FBS; Hyclone, Logan, UT) and 1% penicillin/streptomycin (Gibco, Grand Island, NY). Primary, murine osteoblast cells were isolated from tibial bones of wild-type (wt) and P2Y1/P2Y2 double-knockout (DKO) mice, according to the method described in Ref. 46. Cells were grown in DMEM medium with 10% FBS (Hyclone) and 1% penicillin/streptomycin (Gibco). Cells from passage 1 were used in this study.
Fabrication of 3D bioartificial tissue cultures in Tissue Train culture plates.
Three days before bioartificial tissues (BATs) were made, cells were transferred onto Surflex II culture plates coated with type I collagen (Flexcell International, Hillsborough, NC) at 5,000 cells/cm2 and grown for 72 h to confluence. Osteoblast-populated 3D collagen gels were formed as linear, tethered constructs in six-well, 35-mm-diameter Tissue Train culture plates (Flexcell International) (15). Four plates were situated in one gasketed baseplate that fits on a CO2 incubator shelf. The surface of each Tissue Train culture plate well has a pair of flexible but inelastic nonwoven nylon mesh anchors bonded at east and west poles (Fig. 1). Anchor stems at opposing poles were treated with type I collagen that mechanically bonded to the gel and allowed cell attachment. In preparation for casting a collagen gel, nylon disks with a planar face and a 25 × 3 × 3 mm central trough (loading trough) were placed beneath each well. The central trough aligned with each pair of anchor stems in the Tissue Train culture plate. Holes in the loading trough communicated with a vacuum port, allowing the flexible membranes to be drawn into the trough, creating a space for the cell-populated gel. Cells were grown to confluence on SurflexII culture plates, trypsinized, and counted (model Z1, Beckman Coulter). The type I collagen gel consisted of 70% vitrogen (3.2 mg/ml; Cohesion, Palo Alto, CA), 20% 5× MEM, and 10% FBS. The gel solution was mixed well and adjusted to pH 7.2 using 1 N sodium hydroxide. The cells at 2 × 105 cells/100 μl were suspended in the gel solution, and 100-μl cell-gel mixtures were transferred by pipette into each loading trough of the Tissue Train culture plates. Plates were incubated at 37°C, 5% CO2, for 1 h to allow gelation. Culture plates were then removed from the baseplate, and 2 ml of DMEM medium with 2% serum (in the case of SaOS-2 cells, 10% FBS was used) were added to each well, and the cultures were incubated at 37°C, 5% CO2, for the indicated times.
Compaction kinetics of BATs.
The 3D BATs were scanned each day using a ScanFlex device, an automated image collection system (Flexcell International). The gel areas were measured using SigmaScan Pro 5.0 (SPSDS, Chicago, IL). The compaction curves were plotted as culture area vs. time. The compaction rate was calculated as follows: υ = (S0 − St)/S0 ∗ 100%/t, where S0 is the gel area at time 0, St is the gel area at time t, and t is culture time (day). At the end of each experiment, cells were released from the BATs by incubating with 2 mg/ml type II collagenase dissolved in serum-free DMEM medium at 37°C, 5% CO2, for 30 min. Cells were sedimented and stained with Trypan blue, and viable cells were counted.
Effects of nucleotides and analogs on the compaction rate of BATs.
Nucleotides, their stable analogs, BzBzATP or mATP, were added at the indicated concentrations to each well in the presence or absence of 100 μM PPADS after gelation, and the gel compaction rates were recorded as described above.
Measurement of intact ATP in the culture medium.
Media were removed at indicated time points, and the ATP concentrations were measured using a luciferin-luciferase assay (42).
Assay of cell attachment on type I collagen.
To determine the strength of cell attachment on type I collagen, MC3T3-E1 cells from the BATs treated with or without ATP were released from the hydrogels, as described above, plated on type I collagen-coated slides (Flexcell International) at 10,000 cells/cm2, and incubated at 37°C, 5% CO2, for 1 h. Then the cells were subjected to a steady fluid flow of 50 dyn/cm2 with a computer-controlled FlexFlow device (Flexcell International). Cell images were recorded each minute using a phase-contrast microscope; the number of cells remaining after application of shear stress was counted. The percentage of adherent cells was calculated as Nrem/Ntotal ∗ 100, where Nrem is the number of remaining cells at the indicated time after flow, and Ntotal is the number of total cells before applying flow.
Semiquantitative RT-PCR.
Cells were collected, and total RNAs were extracted using RNeasy Mini Kits (QIAgen, Valencia, CA). cDNAs were synthesized using SuperScripII (Invitrogen/GIBCO, Carlsbad, CA), and semiquantitative RT-PCR was carried out using QuantumRNA classic 18S kits (Ambion, Austin, TX), according to the manufacturer's protocols. cDNA (40 ng) was used in each 10-μl PCR reaction. PCR conditions were as follows: 94°C for 5 min; 35 cycles of 94°C for 30 s, 65°C (P2 receptors) or 50°C (integrins) for 60 s and 72°C for 30 s; 72°C for 5 min. The ratio of pair to competimer was 1:9. Primers used are listed in Table 1. PCR products were separated on 2% agarose gels and photographed.
All experiments were repeated at least three times. Triplicates were used in each group. Statistical analyses were performed using SigmaStat software (SPSS Scientific, Chicago, IL). Significance (P < 0.05, indicated by asterisk) was determined by a one-way ANOVA with Dunnett's t-tests. The values are expressed as means ± SD.
ATP reduced the compaction rate of MC3T3-E1-populated BATs.
Compaction of the gels proceeded over 5 days (Fig. 2), being greatest within the first 24 h. ATP (≥100 μM) reduced the compaction rate in a dose-dependent manner (Fig. 2B). The compaction rates of control and 10 μM ATP-treated gels were the same, with an overall 14 ± 1.0% per day compaction rate. This rate was reduced to 12 ± 0.4 (P < 0.05) and 8.4 ± 0.2% (P < 0.01) per day in the 100 and 500 μM ATP-treated groups, respectively.
Kinetics of ATP hydrolysis.
Extracellular ATP is rapidly hydrolyzed by ecto-nucleotidases secreted by some cell lines (2, 3). To determine the hydrolysis rate of ATP in the culture medium of MC3T3-E1 cells, the concentrations of ATP in the medium were measured at different time points from 0 to 24 h, after addition of 500 μM ATP. Extracellular ATP was hydrolyzed more slowly in the medium from MC3T3-E1 cells compared with the rate reported for other cell lines (7). Nearly 20% of the ATP was not hydrolyzed after 24-h incubation with MC3T3-E1 cells (Fig. 3). These data indicate that MC3T3-E1 cells may express lower levels of ecto-nucleotidases.
Effects of nucleotides on the contraction rate of BATs.
Nucleotides, in addition to ATP, are secreted by cells or arise as hydrolysis products (29). Therefore, the influence of other nucleotides on BAT compaction rate was also measured (Fig. 4). Both purine and pyrimidine nucleotides reduced the compaction rate [an overall compaction rate of 14 ± 1.0% per day for control, 11 ± 0.1% (P < 0.05) and 11 ± 1.4% (P < 0.05)] per day in the presence of 500 μM ADP or UTP, respectively, but ADP and UTP were less potent. To verify that the reduction of the compaction rate induced by ATP was not due to the hydrolysis of ATP or ADP, the effects of ATPγS and ADPβS, the nonhydrolysable analogs of ATP and ADP, respectively, were tested on the compaction rate. Both ATPγS and ADPβS reduced the gel compaction rate [11 ± 0.1% (P < 0.01) per day for ATPγS and 13 ± 0.3% (P < 0.05) per day for ADPβS], and ATPγS showed greater potency (Fig. 5A). To further confirm that the reduction in gel compaction was induced by ATP, the effects of ATP on the compaction of SaOS-2, a human osteoblast cell line, populated BATs were determined (Fig. 5B). The half-life for ATP with SaOS-2 cells is ∼50 s (3). As expected, the compaction of SaOS-2-populated BATs was reduced by ATPγS but not by ATP.
Cell viability in BATs treated with ATP.
Extracellular ATP reportedly induces cell apoptosis (44, 49). To determine whether the reduction in the compaction rate induced by ATP was due to cell death, the cell viability in the BATs treated with ATP was tested by Trypan blue exclusion (Fig. 6). The viable cell number declined from an initial value of 200,000 (200k) cells per BAT to 115k cells per BAT at day 1, then returned to initial values by day 3, and was stable from day 4 onward at ∼170k cells per BAT. There were no dramatic differences between control and ATP-treated groups with respect to viable cell number within the time range in this study.
Recovery of cell contractility of ATP-treated MC3T3-E1 cells after washout of ATP.
An ATP washout experiment was performed to determine whether the ability of cells to compact their matrix was reversible. Extracellular ATP was washed out at different time points after addition of 500 μM ATP from 24 to 96 h (the remaining ATP was refreshed daily) (Fig. 7). MC3T3-E1 cell-driven BAT compaction recovered rapidly and completely to control levels at day 5, if the ATP was washed out within 48 h after addition. Incubation with ATP for 72 h delayed recovery. Cells incubated with ATP for 96 h did not show any recovery of the BAT compaction during the first 24 h post-ATP washout. These results indicate that ATP treatment for up to 48 h did not permanently damage the cells.
Expression profile of P2 receptors in MC3T3-E1 cells.
Due to the cross-reactivity of nucleotides with P2 receptors (23), the response of cells to extracellular nucleotides may be attributed to more than one P2 receptor or be determined by the P2 receptor expression profile. Osteoblasts reportedly express most of the 15 P2 receptors so far identified (3), but the P2 receptor profile varies between primary cells and established osteoblast cell lines, as well as between different nonosteoblast cell lines. Therefore, the P2 receptor expression profile was examined. Eleven of 14 tested P2 receptors were detected (the sequence of mouse P2Y11 is still not available in GenBank) (Fig. 8). Of the known P2X receptors, the P2X4 receptor showed the greatest expression level, P2X5 and P2X7 were at medium levels, while P2X2 and P2X3 were at very low levels, and P2X1 and P2X6 were not detected. Of the tested P2Y receptors assessed, all receptors were detected except P2Y12. The P2Y2 receptor showed the highest expression level in this class.
Multiple P2 receptors are involved in the regulation of BAT compaction by ATP.
Both ATP and UTP reduced the compaction rate of MC3T3-E1 cell-populated BATs (Fig. 4), suggesting that P2Y receptors are involved in this process. To further investigate the involvement of P2X receptors in this process, specific agonists and antagonist of P2X receptors were used: mATP [an agonist for P2X receptors, but not for P2X4 (30)], BzBzATP [P2X4-preferring agonist at low concentration (10)], and PPADS [an antagonist for P2X receptors, but does not inhibit P2X4 (30)] (Fig. 9). mATP did not alter the compaction rate of BATs, while PPADS reduced the rate during the first 48 h. During the first 24 h, the compaction rate was reduced from 54 ± 2.4% (control) to 43 ± 1.2% (in the presence of PPADS, P < 0.01), 40 ± 1.0% (in the presence of ATPγS, P < 0.01), and 29 ± 1.4% (in the presence of ATPγS and PPADS; P < 0.01), respectively. The differences in compaction rates between control and ATPγS-treated, control and PPADS-treated, and between control and ATPγS/PPADS-treated BATs were 14, 11, and 25%, respectively. An additive effect was demonstrated between PPADS and ATP, which indicates that PPADS and ATP reduced the gel compaction rate through different pathways. It was reported that PPADS at 100 μM does not inhibit the P2X4 receptor (but blocks all of the other P2X receptors) (30); a more specific P2X4 agonist, BzBzATP, was used to determine the involvement of P2X4 receptor in this process (Fig. 9B). The results showed that BzBzATP at 100 μM reduced the compaction rate of MC3T3-E1 cell-populated BATs, indicating the potential involvement of BzBzATP-responsive P2X receptors, such as P2X4, in the ATP-induced regulation of BAT compaction rate.
P2Y1 and P2Y2 receptors are not involved in the reduction of BAT compaction induced by extracellular ATP.
Based on the selectivity of P2 receptors for nucleotides (11, 23), the most likely candidates of the P2Y receptors responsible for the regulation of BAT compaction by extracellular ATP are P2Y2 and P2Y4 receptors. To address the question of whether P2Y1 and P2Y2 receptors were involved in this process, an investigation was carried out to test the effects of nucleotides on the compaction rate of BATs populated with primary mouse osteoblastic cells from wt and P2Y1/P2Y2 DKO mice (Fig. 10). Both the wt and DKO cells showed similar compaction rates and similar sensitivities in the response to ATPγS and ADPβS. The overall compaction rates for wt, DKO, wt + ATPγS, wt + ADPβS, DKO + ATPγS, and DKO + ADPβS were 14 ± 1.4, 14 ± 0.8, 8.8 ± 0.8 (P < 0.01), 11 ± 0.3 (P < 0.05), 9.6 ± 0.8 (P < 0.01), and 11 ± 0.8% (P < 0.05) per day, respectively. ATPγS was more effective than ADPβS. These results were similar to those derived from MC3T3-E1 cells and confirmed that both P2Y1 and P2Y2 receptors are not involved in the reduction of BAT compaction rate induced by extracellular ATP.
Extracellular ATP reduced cell attachment on type I collagen.
The steady flow results showed that ATP reduced cell attachment on type I collagen (Fig. 11A). After a 20-min flow, ∼5% of ATP-treated MC3T3-E1 cells remained adherent on the surface, while 40% of control cells remained adherent. Quantitative RT-PCR showed that ATP reduced the expression of integrin α1 by 60% on day 5; the expression of α2, α3, and β1 was not changed (Fig. 11B). The time course showed that the reduction in the expression of integrin α1 occurred from 24 h after addition of ATP and reached the lowest level on day 3 (Fig. 11C).
Bone is a dynamic, living tissue, whose structure and shape are maintained through a tightly coupled process of bone remodeling: bone resorption and bone formation (37). The timing and sequence of these two processes are tightly regulated by various factors (37). The detailed mechanisms are still not clear. However, bone remodeling is regulated by both cytokines and mechanical loading (3, 13). All of these regulators are systemic signals, but bone remodeling is a localized process. How bone tissue converts these systemic effectors to localized signals is not clear. Bowler et al. (3) have reported that extracellular nucleotides could sensitize the parathyroid hormone receptor by interacting with P2Y receptors and therefore augment local cellular responses to parathyroid stimulation in osteoblastic cells. Ostrom et al. (33) also reported that ATP could act as a key determinant in the set point of signal transduction pathways. These studies indicate a possible mechanism explaining how bone cells convert a systemic signal, such as parathyroid hormone stimulation, into a local cellular response. In a previous study, our laboratory (42) has shown that mechanical loading, another systemic stimulus, may be converted into a local response through local elaboration of extracellular nucleotides. The results in the present study indicate that both P2X and P2Y receptors are responsible for modulating the prestress levels of osteoblasts.
ATP release by mechanical loading (fluid flow, tension, and compression) is a physiological phenomenon found in many cell types (7, 33). The local ATP concentration resulting from a nonlytic mechanism can reach the micromolar range in the vicinity of the cell membrane (2). All cells contain a high concentration of intracellular ATP (1–5 mM), as well as the capacity to release ATP (3). In addition, increased blood flow associated with a general inflammatory response could greatly increase the nucleotide concentration to the millimolar level upon platelet aggregation (3). Therefore, at the sites of tissue injury, wounding, or bone fracture, a high local ATP concentration could be present.
Osteoblasts express purinoceptors and respond to ATP (3, 21). Mechanical stimuli induce release of ATP from bone cells (3). Purines and pyrimidines are clearly involved in osteoblast responses to chemical and physical stimuli and share interacting pathways (3). However, it is difficult to clearly address the role of a specific P2 receptor in a given cellular function due to the overlap of reactivity of the ligands for P2 receptors and lack of specific inhibitors for each individual P2 receptor (4). PPADS is a P2X-preferring antagonist, but it does not inhibit P2X4 receptor (30); mATP is an agonist for P2X receptors, but not for P2X4 (30). The PPADS/mATP-insensitive, ATP-induced reduction in gel compaction indicates that P2X4 receptor may be involved. The sensitivity of gel compaction to BzBzATP at 100 μM further suggests the potential involvement of P2X4 receptor. However, most of the available data about P2 receptors were from the studies on heterologously expressed human or rat proteins (30), and it has been shown that P2 receptors from human and rat may respond differently to ATP, even though they shared high homology in amino acid sequences (24). Therefore, without further direct evidence, we cannot prove that P2X4 is the only P2X receptor that is involved in this process: some other P2X receptors, which are not sensitive to mATP or PPADS, but activated by BzBzATP, may also be involved. The sensitivity of gel compaction to ATP, ADP, and UTP indicates that P2Y receptor(s) is (are) also involved. We were not able to define which P2Y receptors may be involved due to the lack of specific P2Y inhibitors. Since the P2Y2 receptor was not involved, the other ATP/UTP-responsive receptor, P2Y4, may be the most likely candidate. However, it was reported that ATP is an antagonist of the human P2Y4 receptor (24). It was shown in the present study that ATP reduced the compaction rate of BATs populated not only with mouse osteoblasts but also with human osteoblasts. Therefore, it is unlikely that the P2Y4 receptor is involved in gel compaction. None of the known P2Y receptors, except P2Y2 and P2Y4, respond to both UTP and ATP (11, 23). Therefore, these results indicate that there may be a novel, unidentified P2Y receptor involved in this process. It was also observed that PPADS alone reduced the compaction rate during the first 48-h incubation. It has been reported that PPADS acts as an antagonist of inositol 1,4,5-trisphosphate (43) and inhibition of phosphatidylinositol 3-kinase blocked FBS-induced collagen gel compaction (48). Therefore, PPADS may reduce the gel compaction rate by blocking the functions of inositol 1,4,5-trisphosphate. However, PPADS did not affect the compaction rate after 48 h, which indicates that different signaling pathways may be involved at the initial stage vs. later stage of gel compaction. This switch may be due to the changes in cell shape and intracellular tension that occur during gel compaction (18).
It has been reported that a cell strain set point is established in cells during tissue development, depending on the local environment and changes during remodeling (1). The mechanism for regulating the compaction of collagen matrixes is complicated and not completely understood (17, 32). It has been reported that PDGF, transforming growth factor-β, EGF, and lysophosphatidic acid stimulate the compaction of collagen matrix by upregulating the expression of integrins (47). Therefore, regulation of integrin expression may be one of the key modulators for controlling matrix compaction. The results in this study indicate that ATP reduced BAT compaction rate by downregulating the expression of integrin α1. There are three different integrins that are responsible for cell attachment to type I collagen: α1β1, α2β1, and α3β1. Several studies have shown that both integrins α1 and α2 play an important role in collagen gel compaction (36, 47). However, in most of the cases, only one of these two integrin subunits was changed at gene expression levels, depending on the cell types used. The results in the present study indicate that integrin α1β1 may be the key regulator of matrix compaction in osteoblasts and may play an important role in bone metabolism. This was confirmed by the gel compaction rate of MG63 [a human osteoblast mainly expressing α2β1 integrin (20)] populated BATs, which was not affected by ATP or ATPγS (data not shown). By reducing cell attachment to matrix, ATP may also reduce cellular responses to mechanical stimuli.
In conclusion, it was shown here that ATP, ADP, and UTP reduced the compaction rate of 3D collagen gels populated with MC3T3-E1 cells. By using specific agonists and antagonist of P2 receptors and primary osteoblastic cells from P2Y1/P2Y2 DKO mice, we showed here that both P2X and P2Y receptors are responsible for the regulation of cell prestress by extracellular ATP in osteoblasts. ATP sensitizes cellular responses to stimuli at lower concentrations and may reduce cellular responses to stimuli at higher concentrations by reducing cell attachment to extracellular matrix. ATP may act as a protective/survival factor in osteoblasts subjected to excessive mechanical loads (33). Since matrix organization is an important step in bone repair/remodeling, the results in this study further confirmed the importance of P2 receptors in matrix organization and compaction during bone repair and remodeling (26). These receptors may represent a new class of drug targets that could be potentially useful in the treatment of bone diseases where remodeling exceeds bone deposition. Moreover, the reversibility of ATP treatment offers a useful means to modulate bone matrix formation in tissue engineering applications.
This work was supported by Flexcell International Corporation, National Institutes of Health Grants AR38121 (A. J. Banes) and HL62584 (J. Faber), and the Hunt Foundation (A. J. Banes).
A. J. Banes is the president of Flexcell International Corporation and receives compensation as such.
We thank Dr. Mari Tsuzaki for assistance with the luciferin-luciferase assay for determination of ATP concentration. We also thank Dr. Carol Otey for critical reading of the manuscript and thoughtful discussion.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2007 the American Physiological Society


text:
Emory & Henry College in Emory, Va., broke ground Friday, April 19, on a $20 million visual and performing arts center that will enhance the College's reputation as a regional leader in the arts and arts education.
The Woodrow W. McGlothlin Center for the Arts honors the memory of a 1937 Emory & Henry graduate and longtime, beloved benefactor of the College. The lead gifts for the McGlothlin Center were provided through the personal generosity of McGlothlin and the McGlothlin Foundation.
The new center will consist of 40,000 square feet of space for a 450-seat theater, a proscenium stage and fly system, a 150-seat black box theater, dressing rooms, production areas, gallery space, a three-dimensional design studio and the College's radio station.
"This is an historic day in the life of Emory & Henry College, a day which many at the College and many in the community have long anticipated." said Dr. Rosalind Reichard, president of the College. "The construction of this important facility marks a new era in arts education at Emory & Henry College, which has long enjoyed a strong reputation for maintaining high standards in the visual and performing arts."
In 1997, Emory & Henry created the Division of Visual & Performing Arts, which brought together existing programs in theatre, music and the visual arts. While pulling these programs under the same division, Emory & Henry also enhanced them to provide a greater number of degrees and to create more opportunities for learning and performance.
The new center is the latest in a series of construction and renovation projects during Reichard's administration. Reichard, who will retire this summer from the College, has presided over approximately $55 million in construction projects, all of which have aimed for high levels of energy efficiency in construction and design.
Woodrow W. McGlothlin served Emory & Henry on the Board of Visitors for 30 years and as president of that board. He taught school and worked in social services before partnering to form what is now The United Company. His belief in the intrinsic value of rural communities and his deep philanthropic and personal commitment to the region and its people drove his generous support of the arts, education and youth programs in the region.
Following the event, the Emory & Henry community celebrated the accomplishments of President Rosalind Reichard, whose era of leadership has been marked by historic levels of campus construction, the development of innovative academic programs, and a dramatic increase in national recognition for the 176-year-old institution.


text: I posted the link in the Links and Resources area for www.thequiltpatternshoppe.com a few weeks ago and fell in love with Jambalaya at the Blue Note Cafe quilt. This was the first time I've ever ordered a downloadable pattern and it was super easy. I pulled out the batiks I have and printed out the pattern. I'm ready to cut tomorrow! I love how this pattern looks and I'm hoping I don't botch it up!


text:
Between a rock and a health reform
There is good reason for that preference. When you are changing the way one-sixth of the American economy is organized and altering life for patients, doctors, hospitals and insurers, you need that kind of a strong launch if it is to survive the inevitable vagaries of the shakedown period.
But getting agreement of Democrats and Republicans on such a volatile issue will not be easy. Republicans and their business allies killed the Clinton effort at health care reform. And even the optimists in the White House acknowledge privately that it will be hard to collect more than a handful of GOP votes in the House, where most of their efforts focus on negotiating agreements between liberal and conservative Democrats.
The two senators who can speak with the greatest authority on framing a bipartisan health care bill are Ron Wyden, an Oregon Democrat, and Bob Bennett, a Utah Republican.
In the last Congress, Bennett, a staunch conservative, delighted the more liberal Wyden by volunteering to become the lead co-sponsor of Wyden�s Healthy Americans Act.
Together, they rounded up the largest bipartisan sponsorship of any major health care bill�eight Democrats and six Republicans, among them three members of the GOP Senate leadership.
Their bill�in simplest terms�would have guaranteed portable, affordable health insurance to every American. It would have required individuals to purchase private health care policies, with subsidies as needed from employers and government. Most remarkably, the sponsors obtained an estimate from the Congressional Budget Office and independent auditors that their plan would be self-financing after a short transition period and might save $1 trillion over 10 years.
When I asked Wyden and Bennett in separate interviews this week how Obama might assemble bipartisan support for any health plan, they both said the key would be to find a plausible way to control its costs.
As Wyden put it, �The country is worried about the amount of debt we�re piling up; you hear it in town meetings and see it in the polls. � That�s why we have to show this is affordable.�
Obama has recognized that need and has offered some tentative ideas for offsetting the costs of covering the 46 million uninsured. But the issue will not really be joined until specific legislation emerges�and is scored by the Congressional Budget Office.
Meantime, there is a preliminary but intense debate shaping up about whether a government-sponsored insurance plan should be made an option for people choosing from a menu of health care coverage options. Liberals want that included. The staff of Sen. Ted Kennedy included it in the draft legislation they rolled out last week. And Obama has reiterated his support for it.
But this week, the Republican members of the Senate Finance Committee, where a bill will be written this month, said almost unanimously that the government plan was a nonstarter.
Bennett, who is not on Finance, underlined that determination, telling me �we will fight almost to the last man and woman against a government-run plan, and not a few Democrats will join us.�
Wyden, careful to preserve his credentials within his own party, said he saw this fight as more of a broad philosophical debate about the role and scope of government, but he reminded me that his bill last year did not include a government-sponsored plan.
The time might come�either before or after the House votes on its bill�when Obama might have to demonstrate his flexibility on the issue of a government-run option. Wyden and Bennett are potential allies if he removes what Bennett calls �the rock� blocking a bipartisan bill. And the president couldn�t wish for better partners.
David Broder is a columnist for The Washington Post. Readers may write to him via e-mail at firstname.lastname@example.com.
Last updated: 10:44 am Thursday, December 13, 2012


text:
Welcome to Martial Arts Do!
Miyamoto Musashi, famous samurai,
swordman and author of The Book of
Five Rings.
swordman and author of The Book of
Five Rings.
This site aims to describe the fascinating world of Martial Arts.
A description that covers not the techniques or sports only, but also the spiritual and psychological details.
As the ultimate goal of most martial arts is not to defeat the enemy, but to find the way to spiritual evolution (known by East Asian traditions as 'Do' or 'Tao').


text: Well, many of you asked (at the recent launch events), so, here you go...I'll be doing my first business intelligence webcast for MSDN on March 21 at noon PST. The topic is implementing SSAS 2005 data mining using Excel 2007 as a data mining client. The format for this live webcast (GeekSpeak - with Glen Gordon and Susan Wisowaty as hosts) is Q&A driven, that is, you ask the questions and I provide the answers. Also, I plan to show attendees a number of demos. Use this Link to register. This webcast will be recorded as well.


text:
Sleek meets chic
From gorgeous gowns to outre ensembles, design diversity rules at Mercedes-Benz Fashion Week in New York.
So far, the collections hint at a feminine, flirty Spring 2013. For more Fashion Week news and views, go to bostonherald.com and find:
• Up-to-the-minute posts from our Stylize bloggers.
- Collections from NY Fashion Week:
|The article you requested has been archived


text:
The Son That Former NFL Player Rae Carruth Tried To Kill Turns 18, Still Suffering From Permanent Brain Damage
The former wide receiver, who plotted the murder of his ex, Cherica Adams, is scheduled to be released from prison soon.
Kyle Glover, son of Tameka Foster and beloved stepson of Usher, passed away on Saturday morning. The 11-year-old suffered critical brain injuries following a tragic Jet Ski accident in Georgia on July 8, and had been on life support since.
TMZ reports that the decision to remove Kyle from life support came after doctor's determined his injuries were too severe to overcome. According to sources, Foster struggled tremendously with the decision and is devastated at her son's passing.
Glover was injured while riding in an inner tube on Lake Lanier, just outside of Atlanta, when he was hit by the Jet Ski. Another child, a female friend of Kyle's, was also on the inner tube at the time of the accident, but she managed to escape with minor injuries.
Authorities are investigating the accident, but it has been confirmed that a friend of the family was riding the Jet Ski at the time of the tragedy. No arrests have been made and it is not being treated as a criminal investigation at this time.
Usher has supported his estranged wife throughout this difficult time, and chartered a plane for Tameka to rush to her son's side as soon as she heard of the accident.
"Usher practically raised Kyle as his own. He's known him for seven years," a source said recently. "He loved Kyle. This is a terrible, awful tragedy. Usher is torn up."
Our thoughts and prayers go out to Tameka, Usher and the rest of the family at this difficult time.
BET.com is your #1 source for Black celebrity news, photos, exclusive videos and all the latest in the world of hip hop and R&B music.
Click here to subscribe to our newsletter.
The former wide receiver, who plotted the murder of his ex, Cherica Adams, is scheduled to be released from prison soon.
Each of the men could be served a life sentence in prison.
Presented by


text:
WASHINGTON, Aug. 28 (UPI) -- Florida voters are fed up with spending by the Obama administration, U.S. Senate candidate Marco Rubio said in the weekly Republican radio address.
Rubio said "out-of-control politicians" in Washington had put the future at risk.
"Like most Americans, Floridians are frustrated with arrogance in Washington," Rubio said Saturday. "They have had enough of the failed stimulus, of record deficits, of reckless spending, and of economic policies that are killing jobs."
Rubio, a product of Florida's politically potent Cuban exile community, is running against Democrat Kendrick Meek and Gov. Charlie Christ, who is campaigning as an independent. The Hill described Rubio Saturday as having the backing of the Tea Party crowd.
Rubio, former speaker of the Florida House, said his immigrant parents toiled at blue-collar jobs to provide opportunities for their children.
"Will we remain that type of country? Well that depends on the decisions we make right now," Rubio said.


text:
Two years after it first appeared, the Conficker worm still poses a threat even though it has been relatively quiet as of late. Since it first emerged, the Conficker Working Group, a team of experts who collectively work to fight the Conficker worm, has had its hands full. While the team has done an admirable job of stifling Conficker's impact, Conficker continues to lurk in the background.
Conficker is a botnet type of malware that infects computers by being downloaded from the Internet or from a mass storage device that has a copy. What makes Conficker especially dangerous is its flexibility. Unlike most other malware that just uses a host computer to send spam or steal personal information, Conficker can be asked to do a variety of things.
Thus far, no one has used the Conficker botnet to carry out any massive attacks. However, its author is still unknown, and the mechanism that would enable someone to seize control of the Conficker botnet is still out there somewhere. Given that the Conficker botnet consists of millions of computers, any attack using the botnet could be devastating.
All evidence seems to suggest that Conficker's author is a malware expert. He or she has constantly made improvements to Conficker's code, repeatedly putting obstacles in the way of the Conficker Working Group. Thus far, Conficker exists in several variants and has spread quite rapidly. Seven million government, business, and home computers in over 200 countries have reportedly been under Conficker's control.
Further complicating things, Conficker is subtle. Since it is not the kind of malware that causes immediate problems for its victims, most users with infected computers don't notice that their computers have been compromised, probably by design. As many malware authors have learned, the malware business is often a matter of finding an optimal balance between stealing as many computer resources as possible and not getting reported. Conficker's author has apparently operated under this notion and perhaps was the first to prove the theory on such a large scale.
There is some good news surrounding Conficker though. Some reports suggest that Conficker is fading, having dropped from first to third on one computer security firm's global top ten threats report in January. Also, it's reassuring to know that it's possible to remove Conficker from infected PCs. Running a reliable antivirus program from a CD rather than from the computer's own hard drive typically does the trick. Alternatively, doing a "clean install" of a system may also be effective, but you'll lose data that isn't backed up. Finally, if you first want to find out if your computer is infected, you can perform this simple test.


text:
From November 2004 to March 2005, on the Ross Ice Shelf, Antarctica, an automated "web cam" was operated on the southward facing lip of a large ice-shelf rift to produce a photographic record of processes active in ice-shelf rift systems. Four times each day, the camera took a photograph in four repeating directions.
Get Data
Data can be downloaded through a Web browser or command line via FTP. When using a Web browser, the FTP link first directs you to an Optional Registration Form that if filled out, will allow you to receive notifications about updates or processing changes related to that specific data set. After completing the Optional Registration Form, the FTP directory becomes available. For additional help downloading data through an FTP client, go to the How to access data using an FTP client support page.
Ice Shelf Rift Time-Lapse Photography, Antarctica, Version 1
Geographic Coverage
|Spatial Coverage:
|Spatial Resolution:
|Not Specified
|Temporal Coverage:
|Temporal Resolution:
|Not specified
|Data Format(s):
|Data Contributor(s):
|Douglas MacAyeal, Kelly Brunt
|Metadata XML:
|View Metadata Record
As a condition of using these data, you must cite the use of this data set using the following citation. For more information, see our Use and Copyright Web page.MacAyeal, D. R. and K. Brunt. 2008. Ice Shelf Rift Time-Lapse Photography, Antarctica, Version 1. [Indicate subset used]. Boulder, Colorado USA. NSIDC: National Snow and Ice Data Center. doi: http://dx.doi.org/10.7265/N5QV3JGV. [Date Accessed].
Detailed Data Description
Digital images are in JPEG format.
Files are named according to the following convention:
Data are organized in folders named for specific camera orientation.
File names indicate date and time of day.
|east green
|camera orientation to east
|west red
|camera orientation to west
|camera orientation to south
|camera orientation to battery box
Approximately 163 Megabytes (MB)
Southernmost Latitude: 78 South
Northernmost Latitude: 78 South
Westernmost Longitude: 178 West
Easternmost Longitude: 178 West
11 November 2004 to 19 March 2005
Temporal Resolution
4 times per day
Parameters include: Antarctica, glaciers and ice sheets
Sample Data Record
Each photograph in the collection is a 640 by 480 pixel image
Software and Tools
Data Acquisition and Processing
The controller board switched on both cameras and initiated a program running on the Linux operating system of the NetCam MP to perform image acquisition, storage and ftp transmission to ftp.thistle.org via an Iridium satellite telephone modem made by NAL. The images used in this study were also stored locally, on a 1 gigabyte flash memory card associated with the Sony camera. Power was supplied by three 100 amp hour 12 V batteries maintained by two 20 W solar panels and a Flexcharge PV7D charge controller.
The camera was operated continuously for 136 days (from 03 November, 2004, until 19 March, 2005) to collect images from 3 standard views: (a) east, along the rift toward its wide, open end, (b) south, directly across the rift, and (c) west, along the rift toward the closed, propagating tip. Pictures of these three views were obtained 4 times a day to allow different sun angles to illuminate the features of the rift walls and snowscape covering the rift floor. The camera system shut down in late March, 2005, because photovoltaic power generation could not maintain the 12 v battery system. The system failed to wake up the following austral spring, and was removed from the field (thus recovering data stored on the Sony flash card) in November, 2005.
The system contained two cameras, a Sony SNC RZ30 (giving a 640 by 480 pixel image using a 1/3-inch CCD) and a StarDot NetCam MP (also giving a 640 by 480 pixel image). The system was controlled by a Rabbit microprocessor located on a custom made circuit board.
References and Related Publications
Contacts and Acknowledgments
Douglas MacAyeal
Department of Geophysical Sciences
University of Chicago
5734 S. Ellis Ave.
Chicago, Illinois 60637 USA
Kelly Brunt
Scripps Institution of Oceanography
University of California San Diego
La Jolla, California 92093 USA
This research was supported by National Science Foundation (NSF) Office of Polar Programs (OPP) grant 0229546 awarded to D. MacAyeal.
Document Information
Document Creation Date
November 2008
NSIDC User Services
Phone: 1 303 492-6199
Email: email@example.com


text:
This was another tea from the Traveling Tea Box. It was interesting and I'm honestly not sure if I liked it, even after two cups. It did not taste like honey, but faintly reminded me of the Crunchie bars in the UK – the ones that have the "honeycomb" center. Not entirely unpleasant, but not quite what I was expecting.
Also, the mate never gave me an energy boost, which made me sad. I really could have used it today in the office.


text:
Dear Newtown:
I must confess that I'm glad to be leaving you — though you're probably even happier to see me, and other people like me, go. As the week goes on and Christmas gets closer, there will likely be fewer members of my profession driving around your town, down Main St., and through Sandy Hook.
You've been incredibly kind. And I would guess that not every one of us has deserved that. But I want you to know that I am very grateful for the patience you displayed when answering my questions, and will always admire the grace with which you handled the terrible events that took place in your pretty town.
I arrived here on Friday night — hours after a troubled man who carried a scary rifle burst into the elementary school and killed 26 of your neighbours, 20 of them children — and went straight to the Catholic church.
Hundreds of you were huddled on the lawn. Originally, I thought the service was over, and people were just waiting for friends or whatever, and were going to head home. Wrong. People were staying. And talking. To each other, to members of the clergy — and to reporters. Tons of reporters. We represented media outlets from around the world. Norway. Spain. Korea, too, I later found out. Canadians, obviously. And from all over the U.S.
And you talked to us. To me. About how you felt about your kids, and how you worried for your friends, and how you hoped your town would eventually be OK. No one was angry at the assailant, not at that point. People were just terribly sad. And even as you cried, and hugged, and sang, only one out of the many people I approached said he'd rather not talk — and he said that with a polite, sad smile. And then he said to me, "But thanks for asking."
I think you wanted to tell your stories, and those of your community, and I believe you did that beautifully.
I've never lived in the United States, but I've spent a lot of time here. For a couple of years I travelled here almost weekly — I was covering professional sports for the Star — and then, when I moved overseas, I worked for an American news organization, with many American colleagues.
I love the U.S., and I love Americans, and I always felt like I knew and understood this place. But here, in Newtown, I was reminded of the differences between our two countries.
It's not just your gun laws, though those are one obvious difference. Put it this way: If I had gone to Newmarket, Ont. — or New Westminster, B.C., or pretty much any other Canadian community — I think things would have been different.
This isn't to say that Canadians aren't just as thoughtful, or as welcoming. But I think we're more reticent when it comes to talking to the press — and nowhere is that difference more obvious than when it comes to public officials.
Just look at the remarkable news briefing held by Dr. H. Wayne Carver, your state's chief medical examiner, on Saturday afternoon. True, there is no prosecution in this case so he doesn't have to be careful about what he says, but I don't think there's any way any Canadian official would get up and speak as frankly as he did.
He said how many times the victims he saw had been shot. He described what the bullets did to their flesh. He gave the kind of detail that sometimes we don't even hear spoken in courtrooms. I was astonished.
And then, I have to confess, I was also taken aback Sunday afternoon when I heard a smart, pretty 21-year-old girl — who was setting up to take donations for the families in your town — make the "guns don't kill people, people kill people" argument.
I'm not naive, but I was surprised when she and her two friends all said, sure, they know people with guns. I don't think I know anyone with a gun (cops notwithstanding). Look around, one of them said to me, waving her arm. Newtown is surrounded by woods. People hunt. Of course they have guns.
Zoe told me her boyfriend's mom and her best friend's mom were in Sandy Hook Elementary when the shooting took place. So I thought she might now think that people don't need weapons.
"Some people are saying this is about gun control," she said. "I don't believe that. This is about one sick person.
"I don't forgive him," she said. "I really don't, at all."
That was about the only anger I heard during the days I spent in your town. Mostly, people talked about love.
I haven't cried yet. I've been close, but when you're working, you just kind of keep on going. I've tried not to look too closely at the pictures of the little girls — they remind me too much of people who are important to me. And I grew up surrounded by amazing women who are teachers.
So I am glad to be leaving you. Because I get to go home and see those people. I'll get to hug them on Christmas Eve, and I'll get to laugh with my girlfriends, and sit at a favourite bar, and leave some of what I heard and saw in Newtown behind.
I won't forget you, though. And when I stop and remember, that's probably when I'll cry.
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|February 16, 2012, 05:39 PM
Senior Member
Join Date: December 5, 2009
Location: Valles Mines, Missouri
Posts: 142
Could this be my 45acp AH-HA moment?
I've been hitting the target fairly good with my GP-100 with homeload 38 specials for quite a while at 10 yards (157 gn home-cast bullet, 3.5 gn Bullseye powder) but with my SA 45, I was all over paper plate size with 230 gn LRN home-cast and 5.0 gn of Bullseye, same awful pattern with store-bought (a lot low-left) and many 'flyers'.
WELL, I ran out of Bullseye and had a can of Clays, so I loaded five 45's with 3.9 grains, they cycled good and had VERY noticeable less recoil and noise,
so I loaded and shot 50 more today, and to my surprise, I'm shooting these 'weak' loads almost as accurate as with the 38spl's. The chrony says they're travelling about 75fps slower than the Bullseye loads (avg 768 fps compared to 843 for Bullseye) but I feel so much more in control.
ALSO, after 50 rounds, all it took was a triple pass of a bore snake to make the barrel all nice and shiny again, NO lead residue and very little powder residue.
Anyone else tried this load? I am a small framed older guy with torn rotator cuffs in both shoulders, so my stance and strength is probably not the best for powerful loads.
Circa 1964: Rocky to Bullwinkle:
"But that trick never works!"
But Bullwinkle tries it anyway...again and again
|February 16, 2012, 06:28 PM
Senior Member
Join Date: May 3, 2009
Location: Central Texas
Posts: 3,560
5.0 grains of Bulll's Eye sounds a bit warm to me. I push the 200 grain LSWC with 4.2 grains of it with exelent results.
No matter how many times you do it and nothing happens it only takes something going wrong one time to kill you.
|February 16, 2012, 06:31 PM
Senior Member
Join Date: December 9, 2011
Posts: 187
^ what he said. if i remember correct im using 4.2 for mine as well.
|February 16, 2012, 08:54 PM
Senior Member
Join Date: February 27, 2011
Location: Dutchess County, NY
Posts: 450
230 gr. LRN, 4.7 gr of Bullseye is my happy place.
|February 16, 2012, 10:23 PM
Senior Member
Join Date: November 6, 2007
Location: Pell City, AL
Posts: 123
4.7 Bullseye 200grn SWC here as well.
NREMT-P AL State EMT-Paramedic ACLS, BTLS, PHTLS, 1991-2006
|February 17, 2012, 08:37 AM
Senior Member
Join Date: December 5, 2009
Location: Valles Mines, Missouri
Posts: 142
My Alliant Reloaders guide lists 230 gn Speer TMJ-RN @ 5.7 max, and a 200 gn. LSWC @ 4.6 max.
Guess my 5.0 is a little hot for the lead bullets, but not jacketed...would explain a lot of scrubbing to clean the barrel, huh?
Circa 1964: Rocky to Bullwinkle:
"But that trick never works!"
But Bullwinkle tries it anyway...again and again
|February 17, 2012, 09:45 AM
Senior Member
Join Date: February 12, 2009
Location: Butte, MT
Posts: 2,010
I found in .45ACP, 5.0g Bullseye under 200g TC bullet very accurate in my Ruger .45 Convertible flattops. It is my go to load in this caliber. No leading either.
5.0g Bullseye, 200g TC, CCI-300, 911fps, 9 SD, 31 ES, 29 shots
A clinger. When guns are outlawed, only outlaws will have guns. Single Action .45 Colt (Sometimes improperly referred to by its alias as the .45 'Long' Colt or .45LC). Don't leave home without it. Ok.... the .44Spec is growing on me ... but the .45 Colt is still king.
|February 18, 2012, 03:39 PM
Senior Member
Join Date: May 21, 2004
Location: Western Penn.
Posts: 377
I've been using 4.grs of Bullseye under a cast 200gr SWC for years. Very mild recoil and accurate to 25yds for me. Some autos may require a softer spring.
For those who've never fought for it, the price of freedom shall never be known...
|February 18, 2012, 05:08 PM
Senior Member
Join Date: May 27, 2007
Posts: 5,106
If you found a load that shoots well stick with it.
If you want to try Bullseye again, cut your loads. A full power load above 800 fps will give me a flinch.
You can cut the 230 lead load way down with Bullseye and the pistol will still function.
I too found the H&G 68 200 LSWC a very accurate load at 4.0 grains Bullseye
Kimber Custom Classic M1911
200 LSWC 4.0 grs Bullseye Mixed cases WLP 21-Jun-06 T = 97 °F Ave Vel = 748.2 Std Dev = 10.86 ES = 41.52 High = 763.2 Low = 721.7 N = 22 Mild recoil, very accurate, excellent target load. 230 LFN Bull-X 3.5 grs Bullseye Mixed Brass WLP OAL 1.20" taper crimp .469" 8-Jan-06 T = 61 °F Ave Vel = 643.6 Std Dev = 14.07 ES = 63.63 High = 679.9 Low = 616.3 N = 32 shot a little high Pistol cycled each shot 230 LFN Bull-X 4.0 grs Bullseye Mixed Brass WLP OAL 1.20" taper crimp .469" 8-Jan-06 T = 61 °F Ave Vel = 715.9 Std Dev = 11.45 ES = 48.32 High = 742.9 Low = 694.8 N = 32 shot a little low 230 gr LRN 4.0 grs Bullseye Mixed Brass WLP OAL 1.250" taper crimp .469" 29-Jan-06 T = 68 °F Ave Vel = 698.8 Std Dev = 10.19 ES = 36.33 High = 713.5 Low = 677.1 N = 28 Little High = put close point of aim V. Accurate 230 gr LRN 4.5 grs Bullseye Mixed Brass WLP 21-Jun-06 T = 97 °F OAL 1.250" taper crimp .469" Ave Vel = 805.2 Std Dev =11.4 ES=54.08 High=836.9 Low=782.8 N =32
If I'm not shooting, I'm reloading.
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I disagree with OP, i think a positive mental attitude does help your physical game. I also think positive attitudes in a particular group can help your game just as really negative attitudes can bring your game down.
Think about the best competitive round of DG you have ever played... How did your cardmates fair? I am guessing they also played relatively well. If for no other reason, I always strive to be friendly and positive so that the people on my card play halfway decent and don't have temper tantrums. ****ty play is almost as contagious as a ****ty attitude.
Even if I am on a card with someone I detest I will help them find their disc or congratulate them on a nice birdie. Outwardly I am continuing my positive attitude for aforementioned reasons, but inwardly I am thinking, sure I will help you find that terrible shot thats costing you strokes, or I am using their good results as motivation to improve my own game.
The bottom line in any tourney I play is that I am there to win, but the only rounds I have ever failed to enjoy were rounds where my cardmates played poorly and I fell into the quicksand of a bad attitude and bad play. If everyone has a good attitude and I play poorly, I chalk it up as a good time and re-focus on practicing and playing well at the next event.
This is for the OP:
Last edited by discmeettree; 05-25-2013 at 08:03 PM.
I think your confusing anger and confidence........ Play confident, not angry.
Every time we step on a teepad to compete we all have 3 battles to fight.
The weather - we have no control over this.
The course - we have no control over this unless you are the one that does maintenance shaping that particular course.
Ourselves - this is the only battle we truly have any control over.
I really like this quote and the insight the author offers below.
"Golfing legend Bobby Jones recognized that being good at golf was not only about having good woods and irons. Said Jones: " Golf is a game that is played on a 5 inch course - the distance between your ears." Bobby Jones had a lot of physical attributes going for him on the course but there is no doubt that he also recognized that golf was in part, a little like chess, and that there was a cerebral element without which the golfer faltered."
The OP here appears to be misguided and somewhat grumpy. The very best players in the world say nice shot when their opponents park a hole and say thank you when their opponents complement their efforts. It all goes back to good ole rule #1 of living a good life: Don't be a d!@k.
It seems like there might be 2 different things here.
You can have a positive attitude, and be a fierce competitor.
Being positive doesn't mean singing cumbaya and wearing flowers in your hair and hoping to lose your card. I'd say that what the OP is talking about is part of having a positive attitude.
But that doesn't mean that you can't also be "professional" about it. I think about McBeth here. The guy's ferocious. I can't think of a worse player to have to face on a final round. But he doesn't seem to have the reputation of being a doosh about it.
Wanting to win is one thing. Wanting your competitors to lose is different. My attitude about that is, I want to win my card against the best possible competition. Better play all around makes me a better player, and if I'm doing my job, I don't need to play head games to win.
I do my best when I look at a competitive round like the blackjack table. Sure, I want to do better than anyone else at the table, but I'm playing against the house, not them, and I can congratulate another winner, if I'm not on the hot streak that night. It's called class, not loser mentality.
I find the best way to play with friends is to let them handle the scorecard and never ask what my score is. I already know how I am playing in my head, but they do not need a reminder that I might be winning, sometimes by a lot. Most of everyone else I play with are of even ability, so I let them fight it out in regards to who is beating who and by how much. I will just play my game and try to beat them quietly; I will let my throws do all the talking for me.
I can still play to the best of my ability but as long as I do not rub in how we are doing overall, we all have fun. Now, rubbing it in how we might have done on a particular hole, that is a different matter.
On a slight side note about being too competitive: Three of us were playing last night at a course we all know very well. We know how fast we can normally play and the course was relatively packed. On a Par 5 where I average 4.5 I was being destroyed by bad luck and just poor technique on my part, luckily limiting the damage to a 7. My friends were circle 7 and double circle 11. The group behind us teed off on us before we cleared the first dogleg and one of them yelled out, "we're playing through on the next hole!" upon seeing my friend splash in the water for the second time.
Even with up to three groups playing this hole at once, there is still a little bit of time after the first group putts out and the second group can approach for a putt. He expected us to wait on the next tee so his group could take another five minutes, hole out, and then tee off in front of us. On our average days we were better players than him and his group, but we were all having a bad round. When his friend temporarily lost a disc on the final approach, he nearly lost his temper because he saw that we were not going to wait for his group to meet us on the next tee. That is being too competitive and not having fun just being out. The weather in Minnesota has been so horrendous recently that any time out is a blessing.
We then strung together three straight good holes, putting us on the final tee box that happens to be next to the tee box that we were supposed to wait for his group to play through. They were just now walking up to that tee box and he was clearly fuming. Why bother playing? If you are taking it that seriously, go to a park where people cannot see you being such an ass.
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Back to New Arcadia, or rather forward to it. New Arcadia is the name of my rather utopian-sounding idea for the 21st Century, We're 14 ½ years into this century already, and don't have much to show for it yet.
But despite the fact that the public has declined thus far to take up the subject for debate and action, time and the forces of history are on my side. The demands for work are shifting inexorably under the relentless and inevitable advance of smart robotics, and we will either adjust to that by creating a beneficial society or a miserable one. Our choice!
Anyway, here's a related proposal, from a somewhat odd source, that at least starts talking about these changes in a forward-looking way. A way to give us back time for pleasurable enterprises. Time for "dreaming up new projects and tinkering." Tinkering! Yes! Is there a sweeter word or a more delightful way to spend time? There is not. But New Arcadia will go far beyond this! It is a whole new way of living life, the one humans have been dreaming of before there were resources enough to achieve it for everyone. Included: a resurrection of locally-based participatory arts, restored ecosystems, and built-environment beauty!
I invite you all to begin tinkering with that.


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text: The Clinton Administration today announced its opposition to two Republican legal reform bills. The legislation would make it harder for plaintiffs to win punitive damages in lawsuits concerning harmful products and would limit the amounts they could collect, while in some cases requiring the losers to pay the other side's legal fees. The Administration described the bills as "too extreme" and "unreasonable" because they would discourage ordinary Americans from suing big companies that could run up vast legal tabs defending themselves -- bills that the consumers might have to pay. House Speaker Newt Gingrich said the legislation would not prevent people from bringing legitimate claims, but would stop them from "blackmailing" companies into out-of-court settlements. The House is expected to vote on both bills this week.


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The Bone Luge: Gross, Weird, But More Than Just a Gimmick
There's a growing trend among North American boozers: sucking down shots through beef marrow bones. Yes, the Bone Luge sounds macabre, savage, disgusting, and fratty, but don't let the name fool you. It's a high-end (read: expensive) affair, but all the pomp isn't just for show. It's actually, you know, good.


text:
SEATTLE -- Ken Hansen, who spent three decades trying to convince the US government that the Samish Indian Nation wasn't extinct and deserved treaty fishing rights, has died, tribal officials said.
Mr. Hansen, 53, who was diabetic and had heart problems and other chronic illnesses, died Wednesday.
He was Samish tribal chairman several times and retired from leadership in October because of his failing health, said Thomas Wooten, who succeeded Mr. Hansen as chairman.
``The tribe is noted for its tenacity and doggedness on issues. That's his legacy," Wooten said Thursday.
Mr. Hansen gained attention in the 1980s when he petitioned the Fish and Wildlife Service for listing under the Endangered Species Act, noting that his San Juan Islands-area tribe and several others had been dropped from a tribe list prepared by a Bureau of Indian Affairs clerk in 1969.
``The whole concept of a bureaucrat in Washington, D.C., telling Indian people that they are not Indians or that they are extinct as a tribe is incredible," Mr. Hansen told the Seattle Post-Intelligence in a 1992 interview. ``It's racist and it's political genocide."
``How do you lose an Indian tribe?" he said.
The Samish, which were federally recognized under the 1855 Treaty of Point Elliott, were also excluded in a federal judge's 1974 ruling on allocation of fishing rights.
Thirty years later, in January 2005, a federal appeals court panel helped clear the way for the Samish tribe to acquire a share of the Washington state salmon catch.
``My only regret is that he won't be there to see us prevail on the treaty rights case" and on the tribe's appeal for compensation for the years when it was delisted, Wooten said.
Mr. Hansen also was known for his leadership among the small tribes of Washington state and worked for other tribes as a consultant and policy analyst, Wooten said.
Mr. Hansen grew up in the Seattle area and became active in tribal politics at age 18. He moved about 20 years ago to Anacortes.


text:
Senate Proposes Historic Plan To Fight Medicaid Fraud
Senator Thomas P. Morahan announced that the Senate Republican Conferenceunveiled the toughest, most comprehensive plan to combat Medicaid fraud in the United States.
The Medicaid Fraud Prevention and Recovery Reform Act of 2006 is a 10-point plan that would fight fraud and abuse at every step of the process, from billing and pre-payment review to investigation, civil recovery and criminal prosecution of Medicaid thieves. The bill was reported out of the Senate Health Committee today and will be acted on by the Senate, in budget legislation and as separate legislation, next week.
"This tough, comprehensive plan will take every possible step to root out fraud, bring integrity and accountability to the Medicaid system and ensure that taxpayer funds are being used properly," said Morahan.
The Medicaid Fraud Prevention and Recovery Reform Act of 2006 includes similar reforms enacted in Texas, that provided immediate results. In the first year after enacting Medicaid reform, Texas increased the amount of money recovered from Medicaid fraud by 30 percent, without incurring any additional expense. Texas, now, annually recoups five percent of its total Medicaid expenditures. Applying the results in Texas to New York's $46 billion Medicaid program, would result in an annual savings of $2.3 billion for the program and provide relief for State and local taxpayers.
The Senate anti-Medicaid fraud plan includes:
Ÿ Creating a new, independent, Office of Medicaid Inspector General by consolidating responsibilities and staff from eight agencies into one new office within the Department of Health;
Ÿ Referring fraud cases to local district attorneys if a case is refused by the Medicaid Fraud Control Unit in the Attorney General's office;
Ÿ Allowing local governments and district attorney offices to share in Medicaid fraud recoveries if they provide information or evidence of fraud;
Ÿ Increasing civil and criminal penalties for people who commit Medicaid fraud;
Ÿ Requiring all health care institutions to implement corporate compliance and internal controls programs;
Ÿ Requiring the State Insurance Department to submit an annual report of health insurance fraud cases submitted by health plans;
Ÿ A $500,000 appropriation for the New York Prosecutors Training Institute to conduct an educational program on Medicaid fraud for local district attorneys;
Ÿ Authorizing the Department of Health to upgrade information technology to detect Medicaid fraud;
Ÿ A demonstration project in Chemung County using the latest technology to detect Medicaid fraud; and
Ÿ Adopting a State False Claims Act that would allow the State to collect 10 percent of the federal share of any recoveries made under the Act.
The federal General Accounting Office estimates that 10 percent of Medicaid expenses are diverted through fraud, an amount equal to billions of dollars spent by New York on the program. By some estimates, fraud may account for as much as 30 percent of Medicaid costs.
The comprehensive Senate Medicaid fraud plan was developed after statewide public hearings held by the Senate Medicaid Reform Task Force. At the hearings, the task force received input and suggestions from people in the health care industry and the law enforcement community on what could be done to strengthen the state's efforts to detect and prevent Medicaid fraud.
Among those who testified at the hearings was Texas Health and Human Services Commission Inspector General Brian Flood, who spoke about the remarkable results of Texas Medicaid fraud plan, upon which the Senate plan is modeled.
In 2003 the Senate Medicaid Reform Task Force recommended several important measures that have become law, including the State cap on local Medicaid expenses and the State takeover of the local share of the Family Health Plus program, that have saved local property taxpayers billions of dollars.


text:
On Sun, 2003-11-02 at 15:45, wsa wrote: > Alvin Oga wrote: > > > /boot is NOT needed ... - /boot was needed in the old days to > > guarantee that the > > boot kernel was occupying the 1st 1024 cylinders > > So where do the kernels go when you don't have a /boot partition? > I'm now using a seperate /boot partition but it's full now. > So is it possible to change this? There are also other reasons to use /boot other than to keep the kernel @1024 or below cylinders. I make my /boot 150MB including any journal-ling. Therefore, I get to only have ~ 25 Kernels in place before I have to start cleaning. There are other reasons as well, like during previous upgrades the entire "/" filesystem goes belly-up... where would you be then? Me, I just boot into Single user mode and fix it. It allows flexibility. It also allows for cleaning up after a simple mistype of a command at an inopportune time. I believe in the build it once regimen. I have transferred Debian Builds from one machine to another to another to another... or even copied those builds... and so on. I hate to install, but if I do, it's always in a chroot'd environment from a bootable CD of Linux some how. -- greg, email@example.com REMEMBER ED CURRY! http://www.iwethey.org/ed_curry Your eyes show as many deep and full shades of blue as a healing bruise upon an injured forelimb.
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Stephani Satterfield and John Major ruefully sipped sodas Saturday on the University of Georgia's nearly deserted North Campus.
"I think it stinks," Satterfield said when asked about tailgating in one of UGA's two alcohol-free "family-friendly zones."
"We have this beautiful campus that alumni support, our taxes support, and people are packed in downtown because we're told we can't drink," the Cumming computer consultant said.
Two football games into UGA's experiment with banning alcohol in two grassy quads on the north and south sides of campus, fan reaction is mixed, and the no-drinking zones haven't caught on.
In contrast to years past, when North Campus was prime real estate for tailgating, the area was practically empty Saturday morning.
Satterfield said a video is circulating on the Web site YouTube of a nearly vacant North Campus before the season opener versus Western Kentucky two weeks ago, set to the Three Dog Night song "One Is the Loneliest Number."
Satterfield's friend John Major, a Cherokee County teacher, said the group only continues to tailgate on North Campus because it's tradition.
"We've always tailgated here the past four or five years, and we decided not to stop," Major said.
Katie Anderson, a teacher's aide in Newnan, also decided to stick with her traditional spot near UGA's main library. But Anderson said the no-alcohol rules are good for her 18-month-old daughter, Sarah Kate.
"It's nice not to be around rowdy people," she said.
Pete Wilbanks, a professor at an evangelical college in Greenville, S.C., is an even bigger fan of the family-friendly zones. Wilbanks, watching his two sons shoot basketballs in a playground area, said he comes to one or two games a year, but is considering coming to more because of the new rules.
"Knowing they have this alcohol-free zone would make me think about getting on the season ticket waiting list," he said.
A few fans admitted to sneaking a bit of the stronger stuff into their cups of cola Saturday, and Tim Stewart, an Athens-Clarke police detective patrolling on campus, said the alcohol rules aren't strictly enforced.
Police aren't checking cups, but if officers see someone who obviously is drinking alcohol from a can or bottle, they will tell him to pour it out or leave the area, he said.
Stewart said he's gotten positive feedback about the family zones, and that fans who've been caught drinking have been cooperative.
"It hasn't been a problem," he said. "Any time somebody has an open container, you tell them and they get out."
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Hi Guyes,
I m using nero 7 esssential (It's came with DVD RW drive when I bought).
1) I've copied 3 movies with all necessary files (.VOB, .IFO, .BUP) from a DVD Disc. The total size 5.64 GB.
But I want to write in a DVD-R/DVD+R (4.7 GB) using nero. How can I compress the files so I can burn in 4.7 GB?
Is it possible with nero 7 or I need to use extra soft? please advice...
2) If I copied only the .VOB files & write in a new disk manually with nero, then the embaded subtitles are lost. But I want to keep it. How can I keep the subtitles?
Any one please advice...
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You can use Nero Recode (essentially DVD Shrink in a new dress) to make it fit, then use Nero to burn. If you don't have Recode, use DVD Shrink (or better yet, DVD Rebuilder) to do all the work, then burn.
Ok Guns1inger, thanks for reply...
I'll try & inform later...
Actually I m really new in this topics, so need to know many info...
Another thing is that: After play DVD movie, we get a link page (in .VOB format). How can I make/Edit this image as per my requirements & make links also to movie (play/chapters...etc) with my .VOB files? Is it possible with DVD rebuilder?
The process you are talking about is called authoring. It is easier to use VOB2MPG to extract the video/audio stream from the VOBs, then author a new disc with something like GUIForDVDAuthor (free) or Tmpgenc DVD Author (not free), that to try and add menus to an existing VOB set. Tmpgenc can probably import the VOBs directly.
DVD Rebuilder is only for re-encoding a movie to make it smaller.
Hello Guns,
Many thans to you for replying...
I downloaded "DVD-RBv0982", install it. Here is the total description of my tasks:
I just copy & paste these DVD files in a separate folder in hard drive named "Test1" - VIDEO_TS.BUP/VIDEO_TS.IFO/VTS_02_0.BUP/VTS_02_0.IFO/VTS_02_1.VOB/VTS_02_2.VOB.
Totaly 1 Movie with all the necessary files from disk - 1.17 GB. I want to compress this files for testing purpose.
But I am getting these 3 errors & I cant understood. please see the error .doc file.
The first one is telling you that you don't need to compress the files because they already fit on a single layer disc. DVD-RB, DVD Shrink and Nero Recode are all designed to shrink a DVD9 down to DVD5 size.
The second one is telling you that your DVD is incorrectly structured. Your original DVD should have had a folder called Video_TS, which has all the files for the DVD in it. This is what you should have either copied (for a non-protected disc) or ripped (for a protected disc) to your HDD, and is what you should have pointed DVD-RB at.
I haven't seen the third error before, but would guess that it is probably related to the second one.
If you want to test these programs, rip a full commercial disc and let them work the way they were designed.
Hi Guns1,
I've installed DVDShrink 3.2. I've a DVD disc with 4 movies & total size 7.81GB. But I need 3 movies from that disc & write in 1 DVD-R disk 4.37GB. So, I've tried to reduce the total disk with DVD shrink (100% ratio, autometic).
But, It reduced to 6.51 GB as back up. So, I delete 1 movie which I dont need. Finally it contains 4.68 GB.
But I need lower then 4.37GB, how can I manage this? So, that I can write in my disc?
Hi Guns1,
No solution???
Because of the way DVD Shrink reduces the size, it has limits. You can run the files through Shrink a second time, however the reduction you are looking for is going to produce low quality.
DVD Rebuilder is by far the best solution for what you are trying to do. However if your computer specs are correct, expect it to run all night.
ya, I tried a movie with rebuilder, but it took few hours just with 1 movie, so i just reject it.
DVD shrink is much better & time saving with limitation. but, I can't reach my target!!! I'll try 2nd time & let u know...
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text:
Sidebottom pegs back Kiwis
Ryan Sidebottom led the way as England restricted New Zealand to 282-6 on the opening day of the series at Seddon Park.
By Graeme Mair
Last Updated: 06/03/08 5:32am
England's bowlers worked hard to restrict New Zealand to 282-6 on the opening day of the series in Hamilton.
New Zealand opted to bat first and looked to be setting the platform for a large total after reaching lunch at 87-1 on a slow pitch that offered no encouragement to bowlers.
The tourists - led by the exemplary Ryan Sidebottom - stuck to their task and gained a foothold in the contest by removing Stephen Fleming (41) and Matthew Sinclair (8) during the afternoon session.
And the Black Caps found themselves in danger of wasting their solid start when Jamie How fell eight short of a century and Jacob Oram followed cheaply to leave them at 191-5 early in the evening session.
However, Brendon McCullum blasted a 55-ball half-century to regain the initiative for his side during an 86-run, sixth-wicket stand with Ross Taylor (54no) that came off just 19 overs.
But Sidebottom had McCullum caught behind during the penultimate over to tilt the momentum of the game just in England's favour.
Bell injury
That scenario had looked unlikely during a wretched opening session in which England wasted the new ball and then had Ian Bell taken to hospital.
Bell, fielding at short-leg, was hit on the right hand while trying to avoid a full-blooded pull by his New Zealand namesake Matthew in the 11th over. He was immediately taken to hospital where initial X-rays showed no broken bones although it is not yet clear if he will be able to bat during the remainder of the match.
It was Steve Harmison (1-64), operating mainly in wayward mode, who made the only breakthrough of the morning in his second over, the 14th of the day. Bell (19), undone by overconfidence having hit the previous two deliveries for four, edged a flat-footed drive to Alastair Cook in the gully.
Once the new ball's shine had begun to wear off, the slow-paced, flat nature of the pitch became increasingly apparent as Fleming dominated a second-wicket stand of 66 with How either side of lunch.
Fleming, who plans to retire at the conclusion of the series, looked in fine touch while racing to 41, including eight boundaries. But his dismissal, brilliantly caught one-handed by a diving Cook in the gully to give Sidebottom just reward for a parsimonious post-lunch spell, opened the door for England.
And when Sinclair chipped the tamest of return catches to Paul Collingwood (1-16), New Zealand were 129-3 and looked to have squandered the advantage of batting first.
However, the hosts' innings was steadied by a fourth-wicket alliance of 47 between How - continuing his steady, unobtrusive progress - and Taylor, who mixed watchful defence with the occasional meaty drive.
The runs dried up after tea as both batsmen fell into an almost exclusively defensive mindset, particularly against the left-arm spin of Monty Panesar.
How was within eight of a maiden century when he was undone by a Panesar delivery that turned and took the outside edge on its way low down to Collingwood at slip.
England's superb catching was another positive to take from the day, Cook completed a personal hat-trick by holding on to a diving chance to dismiss Oram (10) and give Hoggard (1-95) his first wicket.
That brought McCullum to the crease with his side under pressure at 191-5 and more than 20 overs remaining in the day.
What followed was further evidence of his fabulous talent as, for the only time, England lost control in the field.
McCullum carried on from his exploits in the one-day series with a 55-ball cameo that featured two sixes and five fours and swung the momentum back New Zealand's way. Taylor also joined in the fun and reached a half-century of his own - his first in Tests - from 118 balls.
But Sidebottom (2-39), armed with the second new ball, had the final word by tempting McCullum (51) into edging a wide delivery to debutant wicketkeeper Tim Ambrose as New Zealand closed at 282-6.


text:
Moves like Jagger
The supermodel-of-the-moment with genes to make anyone jealous, reveals her secret career ambitions. And it's not on the catwalk.
Supermodel Georgia May Jagger has revealed her secret desire to be a veterinarian. The animal lover said that she grew up wanting to help her four-legged friends and would consider pursuing a career in the industry when she retires from the runway.
"I wanted to be a vet when I was growing up, so I can definitely imagine doing that," she told Vogue.co.uk. "I love animals. I also really enjoy photography, so I would like to do more of that in the future."
Her newest campaign for Hudson jeans is scantily clad as expected, as she shows off her incredible figure.
"I really love the Hudson brand because they have the classic jeans but they have a lot of fun with the denim, too," she said. "I'm really big on colour and I love that they have a lot of great colour options, as well as the black and indigo skinny jeans that I like to wear every day. I have also been working with them for a long time now and they have an incredible team who I really enjoy working with!"
And the daughter of Jerry Hall and Mick Jagger gave posing advice on how to look good in front of the camera. "I always say try and be yourself and do what feels natural," she said. "If it doesn't feel good, it most probably doesn't look good either. I think a smile always helps, too."
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