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Tarun Chakrabarti
January 3, 2015 · Public
Hi Bucky I have question for you.
Let me put in this way, I am very new to web-development. I have learnt Xhtml, html5, css, css3, basic of javascript. I learned all this from Udemy, infinite skills, learnable etc. Basically every time I see any course under five dollars, I buy it and finish it. But most tutorial does not teach how they initiate the design, where they are going to get the contents, there are very less exercise project to even practice before get into the field. I mean i know all the html tags css css3 little bit of javascript, so now what go and tell people that I can build you a web site, of course not, also there are not much tutorial about what is after javascript basic, oh yeah do the advance tutorial but no tutorial on putting every thing like html css js all together making a full website, I mean even I have sit for constant 3 to 4 day to finish the tutorial I will do it but there are none. Can you please help me out, cause I need get better at this and start working as freelancer but right now I am confuse what to do? where to go? what next to learn? please help! I would be grateful to you.


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This was fantastic fun to solve, Sue--thanks so much! 1:08:24.
Have a good time whatnauts.
Oh goody, goody - it's been awhile since you posted one of these!! Thanks, Sue.


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Netanyahu on Mass Budget Cuts: Security Is Israel's Top Priority
After Israel-Gaza fighting, cabinet approves 2% budget cut to all ministries; says will provide IDF with immediate allocation of 1.5 billion shekels to cover some costs of Operation Protective Edge.
Cabinet ministers approved mass budget cuts on Sunday to cover the cost of the recent military operation in Gaza.
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ClamCase releases new iPad Air ClamCase Pro
- Written by Eric Blair
- Published in Tablet/ E-reader news
ClamCase today announced the release of a completely redesigned ClamCase Pro for the new iPad Air. The reimagined all-in-one keyboard case and stand for iPad has been thoughtfully refined to deliver even more enhanced features, protection and productivity
Built with even more features, the iPad Air keyboard case is the thinnest and lightest ClamCase. The 360˚ hinge provides a whole new way to hold the iPad. The bead blasted aluminum and white polycarbonate enclosure creates a durable shield. Users can type faster, more accurately and longer than before with the elevated island style keyboard. The upgraded battery life allows for months of use on a single charge.
ClamCase, a consumer electronics design company based inHollywood, California, manufactures and distributes the iPad keyboard cases and peripheral devices. ClamCase products include features designed to enhance FaceTime, Facebook, YouTube, Netflix, iMessage, online gaming, and many other apps and web-browsing. In addition to the growing popularity with professionals, educational institutes, and other organizations, ClamCase products are used to protect the iPad, while increasing its mobility, efficiency and productivity.
The ClamCase Pro is available now at ClamCase.com.


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Here is the latest Oregon news from The Associated Press
ALMA, Mich. (AP) – Police in Oregon are making another try at solving the deaths of two farmworkers in an arson fire that destroyed a cheap hotel 39 years ago, circulating a photo of a man who claimed to have Michigan ties and who fled after being questioned. Salem police investigator say the man disappeared without a trace after police interviewed him in 1975. It followed a fire at the Bligh Hotel that killed 49-year-old residents Arnold Stover and August Cico (CHEE'-koe).
SALEM, Ore. (AP) – A recent change in the winds could signal the end to a spike in marine trash on Oregon and Washington beaches. Researchers at the National Oceanic and Atmospheric Administration say an annual transition from wet winter lows to dry summer highs has brought the warmer, northwesterly winds that push debris-laden surface water back out to sea.
GRANTS PASS, Ore. (AP) – A new study suggests steelhead trout can have trouble using the Earth's magnetic field to navigate if they were raised in a hatchery, where the field may be distorted by iron pipes. The lead author says the results may explain why so many hatchery fish stray from their native rivers and have a lower rate of ocean survival than wild fish.
KLAMATH FALLS, Ore. (AP) – Drought is again gripping the high-desert Klamath Basin, and the holders of water rights are submitting claims for surface water. It's the second year that the basin has been subject to a new state-level determination of how water gets allocated, based on the age of the claims.
Copyright 2014 The Associated Press.


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This study reports development of a novel method for high-resolution in vivo imaging of the function of individual mouse retinal ganglion cells (RGCs) that overcomes many limitations of available methods for recording RGC physiology. The technique combines insertion of a genetically encoded calcium indicator into RGCs with imaging of calcium responses over many days with FACILE (functional adaptive optics cellular imaging in the living eye). FACILE extends the most common method for RGC physiology, in vitro physiology, by allowing repeated imaging of the function of each cell over many sessions and by avoiding damage to the retina during removal from the eye. This makes it possible to track changes in the response of individual cells during morphological development or degeneration. FACILE also overcomes limitations of existing in vivo imaging methods, providing fine spatial and temporal detail, structure-function comparison, and simultaneous analysis of multiple cells.
- retinal ganglion cells
- in vivo adaptive optics imaging
- calcium imaging
available methods for examining the physiology of retinal ganglion cells (RGCs) have marked limitations, which are resolved by the novel in vivo imaging method described here. Existing in vivo methods study cells in a relatively unperturbed state, but the physical challenge of recording within the eye often limits recording to a small number of cells (e.g., Martin et al. 2001), and repeated study of individual cells is not possible. In vitro recording is a highly efficient approach, imaging or recording electrophysiological responses from many cells simultaneously (e.g., Borghuis et al. 2011; Chichilnisky and Kalmar 2002), but it has two major limitations. It does not permit long-term study of the same retinal neurons and circuitry over time, for example, the study of how neurons degenerate or how they change during development. Also, in vitro methods do not avoid possible artifacts due to retina removal itself, for example, the effect of cutting the axons of ganglion cells (Lukas et al. 2009; Mandolesi et al. 2004).
Here we demonstrate an in vivo imaging method, FACILE (functional adaptive optics cellular imaging in the living eye), that can repeatedly image the light response of ganglion cells in the eyes of living mice. This method shares the advantages of other in vivo techniques in studying retinal cells in an intact state, and it can track the response of individual cells over days or weeks. Like other imaging methods, FACILE permits the study of many cells simultaneously, but unlike low-resolution imaging (Prilloff et al. 2010; Sabel et al. 1997), it provides both subcellular resolution and high sensitivity. Fluorescent adaptive optics (AO) imaging has previously been used to image the morphology of retinal ganglion cells expressing fluorescent protein in living rodent eyes and characterize their dendritic stratification (Geng et al. 2009, 2012). Here, to study ganglion cell physiology, we expressed the genetically encoded calcium indicator (GECI) G-CaMP3 (Tian et al. 2009) in retinal ganglion cells and tracked the calcium response of individual cells to visual stimuli. A challenge of in vivo fluorescent imaging is that it uses visible light that strongly light adapts the retina. For example, in this study the excitation wavelength was 488 nm, which is close to the peak sensitivity of mouse middle-wavelength sensitive (M) cone opsin and rhodopsin (Jacobs et al. 2004; Lyubarsky et al. 1999, 2004). To separate visual activation from response to the imaging light, we used ultraviolet (UV) light (365 nm) to stimulate the retina thus selectively activating the short-wavelength sensitive (S) cone opsin, which activates most retinal ganglion cells (Wang et al. 2011).
Because in vivo imaging is intrinsically photon limited, we used a viral-vector-mediated gene delivery that leads to a high level of G-CaMP3 expression in cells without damaging the cells. The rabies vector produced intense G-CaMP3 expression in individual ganglion cells without nonspecific background expression, which improved the signal-to-noise ratio of imaging. As described below, rabies mediated G-CaMP3 expression produced stable physiological responses in cells over the first 4–8 days of imaging, which then declined as the cells began to degenerate.
Intracranial injection of rabies vector.
The rabies vector was produced in the laboratory of Dr. Edward M. Callaway (Salk Institute for Biological Study, San Diego, CA). In brief, G-deleted rabies viruses are generated in B7GG cells, concentrated by two rounds of centrifugation and titrated in HEK293t cells as described previously (Osakada et al. 2011). The titers of the viruses used in this study were 4.8–8.0 × 108 infectious units/ml. The injection procedure was as described previously (Cetin et al. 2006) and followed the guidelines of Biosafety Level 2. Typical coordinates of injections into superior colliculus were 3.8-mm posterior to Bregma, 1.0-mm lateral, at two depths of 1.4 and 1.7 mm (Keith and Franklin 2007), and the injection volume was 0.5 μl at each depth. During injections, the cornea was lubricated with GenTeal (Bausch & Lomb, Rochester, NY) to prevent cataract formation. AO imaging began 4 days after injection to allow recovery from surgery, as well as ganglion cell expression of G-CaMP3. Wild-type C57BL/6J mice of both sexes were used. All animal procedures were conducted according to the Association for Research in Vision and Ophthalmology Statement for the Use of Animals and the Guidelines of the Office of Laboratory Animal Care at the University of Rochester. The protocol was approved by the University Committee on Animal Resources of the University of Rochester.
AO imaging.
Before AO imaging, the retinal distribution of transduced ganglion cells was identified on low-resolution fluorescent images of the mouse retina, obtained with a fundus camera (Micron III, Phoenix Research Laboratories, Pleasanton, CA) or confocal scanning laser ophthalmoscope (SLO; Spectralis HRA, Heidelberg Engineering, Heidelberg, Germany). FACILE was done with the mouse fluorescence AO SLO built at the University of Rochester. A detailed description of this system and characterization of its performance for imaging various retinal structures have been reported elsewhere (Geng et al. 2012). Briefly, during imaging, we captured two channels of images simultaneously: a reflectance image of the retinal structure (e.g., vessels in the inner retina), using infrared light with center wavelength 790 nm and a fluorescence image of the G-CaMP3 expressing ganglion cells (excitation: 488 nm and emission: 525 ± 17.5 nm; FF01–520/35–25; Semrock, Rochester, NY). Infrared light of 850 nm was also used for wavefront sensing to correct the optical aberrations of the mouse eye. All imaging lights, 488, 790, and 850 nm, were scanned over a 6 × 5° rectangular region on the retina, and AO images were taken over a central 5 × 5° square, where illumination was more uniform. The typical intensities of the imaging lights at pupil were ∼100 μW (488 nm), ∼150 μW (790 nm), and ∼25 μW (850 nm).
Eye motion was calculated from high signal-to-noise ratio reflectance images of retinal structure (e.g., vasculature), and the motion correction applied to both channels. Images of both channels were captured at 25 Hz. The gain of the fluorescence channel was set to fully utilize the dynamic range of the system, with only a small percentage of pixels being saturated. For navigation across the retina, the retinal vasculature visible in AO reflectance was compared with low-resolution, large field fundus images.
Presentation of UV light.
The primary visual stimulus was 365-nm UV light. The UV light was generated by an LED that produced a peak wavelength of 365 nm (M365L2-UV; Thorlabs, Newton, NJ) and was presented to the eye in Maxwellian view over a circular region of ∼24° diameter on the retina. The intensity of the UV light at pupil was 20 μW.
Visual impact of imaging and visual stimulation lights.
To compare the visual impact on photoreceptors of the various imaging and stimulation lights, we calculated isomerization rates for mouse M and S pigments and rhodopsin using methods described by Naarendorp (2010) (see also, Wang et al. 2011) (Fig. 1, A and B). The spectral sensitivities followed the template developed by Govardovskii et al. (2000) using λmaxs of 508, 360, and 498 nm (Jacobs et al. 2004; Lyubarsky et al. 1999, 2004). Pigment self-screening was calculated based on inner segment lengths of 13.4 um for cones and 24 um for rods measured by Carter-Dawson and LaVail (1979) and an axial specific density of 0.015 um−1 for cones and 0.019 um−1 for rods (Naarendorp et al. 2010). The end-on collecting area of mouse rods and cones were 0.85 and 1 μm2 (Naarendorp et al. 2010). Preretinal absorption of the mouse eye was estimated from Henriksson et al. (2010). To simplify the calculation, all light sources were treated as monochromatic light, with all the power at the spectral peak. To estimate the bleaching effect of imaging lights, we assumed a total 7.4 log10 pigments in a M cone (Fu 2011), estimated from cone outer segment volume of 14 μm3 (Carter-Dawson and LaVail 1979) and a pigment concentration of ∼3 mM (Harosi 1975). Psychophysics Toolbox extensions (Brainard 1997; Pelli 1997) were used for calculating isomerization rates. Although the UV stimulus light produced visible autofluorescence at the contact lens surface, the confocal AO system eliminated this fluorescence signal.
Experimental procedure.
The primary stimulus used to activate ganglion cells was an 8-s steady presentation of 365-nm (UV) light, which alternated with 8 s of no UV light. For a few measures other stimuli were used; 1-s steady presentation of UV light, alternating with 15 s of no UV light or onset of the 488-nm imaging field.
To minimize retinal exposure to blue light, the 488-nm imaging light was extinguished between each imaging block. At each retinal location, an imaging block was of an ∼3-min duration, with at least 1 min between recording blocks. Baseline fluorescence generally decreased over the first 30 s of each block; thus data analysis excluded the initial 30 s of each block.
Measurement of fluorescence intensity of ganglion cell somas.
Fluorescent measurements were made from motion-corrected (registered) fluorescent videos, using Fiji (Schindelin et al. 2012), a distribution package of ImageJ (W. S. Rasband, ImageJ, U. S. National Institutes of Health, Bethesda, MD; http://imagej.nih.gov/ij/, 1997–2011). For each registered fluorescent video, round or ellipsoidal regions of interests (ROIs) were placed on each ganglion cell soma identified in the summed image of that video (see Fig. 2). As a measure of background, a rectangular ROI larger than a typical ganglion cell soma was placed at a location where there were no visible ganglion cell somas. Fluorescence intensity was measured within ROIs for each frame of the video, generating a temporal sequence of fluorescence intensity for each identified ganglion cell soma.
To improve the signal-noise-ratio of the measurement (as illustrated in Fig. 2B, left), we binned 10 successive samples, reducing the temporal sampling rate from 25 to 2.5 Hz, a rate that is fast relative to the calcium response of ganglion cells. A time stamp was recorded for every video frame during image capture to correctly align each frame with the timing of stimulus presentation.
Measurement of peak response from normalized fluorescence.
We calculated fluorescence response (ratio F/F0) by normalizing measured fluorescence (F) of each stimulus cycles to the mean baseline value (F0) obtained from the time preceding the onset of the UV flash (2–0.4 s before onset). Time of the peak response was identified from smoothed data with a three-sample moving boxcar average, and the peak response amplitude was determined as the mean of three consutive time points statistically different from baseline (F/F0 = 1), using a one-tailed t-test. All data analysis used MATLAB (MathWorks, Natick, MA).
Evaluation of light safety with repeated imaging.
Total light exposure included the imaging lights, primarily 488 and 790 nm, and the visual stimulation light, 365 nm. Exposure to the imaging lights did not exceed 1.7 times the American National Standards Institute (American National Standard for the Safe Use of Lasers ANSI Z136.1–2007) maximum permissible exposure (MPE) for human retina (Delori et al. 2007) scaled by the ratio of the numerical aperture (NA) of human to mouse eyes (NAHuman2/NAMouse2 = 0.2) (Geng et al. 2012). This exposure level was considered unlikely to be damaging, given that the MPE is set 10-fold below minimum damage thresholds to ensure safety. Maximum radiant exposure to UV light for each eye, each day was 0.18–1.02 J/cm2. This value cannot be compared with human safety limits since there is no MPE standard for UV light, but this light level is of concern, given that it approaches the value that has been shown to produce retinal damage in rat (Busch et al. 1999; Gorgels and van Norren 1995; van Norren and Gorgels 2011). Despite this concern, no photoreceptor alteration or loss was found in AO imaging at any retinal location on any of the imaging sessions over 7 days nor was there any visible change in cornea or lens. Our previous studies have demonstrated that AO imaging is a highly sensitive indicator of light damage, showing retinal changes in monkey retina at light levels well below ANSI MPE standards for light safety (Morgan et al. 2008).
Combining calcium imaging with in vivo fluorescent AO imaging.
This study combined a previously described method for high-resolution in vivo AO imaging of reflectance and fluorescence signals from mouse retina (Geng et al. 2012), with insertion of the genetically encoded calcium indicator G-CaMP3 into RGCs (Tian et al. 2009). G-CaMP3 was expressed in mouse RGCs by injecting glycoprotein-deleted rabies vector (Osakada et al. 2011), carrying the G-CaMP3 gene, intracranially into mouse superior colliculus, producing RGC transduction in a few days. The apparatus and method for AO imaging were as previously described (Geng et al. 2012) with the following exceptions. The imaging laser in the present study was 488 nm rather than 514 nm, chosen to match the excitation of G-CaMP3, and lower laser intensities were used in this study to maintain safe light levels during the extended imaging needed to characterize physiological responses (see materials and methods).
Visual stimuli were 488 nm (blue) or 365 nm (UV).
To excite retinal neurons we initially used the onset of the 488-nm (blue) imaging field, a wavelength near the peak sensitivities of both mouse M cones and rods (Figs. 1A and 2A). However, to minimize interactions between the stimulation and imaging light, most measurements in this study were made of responses to 365 nm (UV) light (Figs. 1A and 2B), obtained in the presence of constant 488-nm illumination. As shown in Fig. 1A, 365-nm light is near the peak sensitivity of mouse S cones (Jacobs and Rowe 2004; Lyubarsky et al. 1999; Nikonov et al. 2006) and activates both S cones and the many cones that coexpress M and S opsins (Applebury et al. 2000; Haverkamp et al. 2005; Jacobs and Williams 2007; Rohlich et al. 1994; Wang et al. 2011). To reduce the visual impact of the 488-nm imaging light on S cones, the intensity of the UV stimulus was set ∼3 log10 units above that of the 488-nm fluorescence imaging light (Fig. 1B).
Calcium responses to brief stimuli.
We examined the time course of calcium responses to brief stimuli, initially to onset of the 488-nm imaging field. Figure 2A illustrates the G-CaMP3 response of a ganglion cell in the living mouse eye to onset of the 488-nm imaging light. The response (blue curve) showed a quick rise to peak, followed by a slower decay to baseline within 10 s. This response demonstrates that the 488-nm imaging light activates retinal neurons, suggesting that calcium responses might best be understood if the 488-nm light was held constant. Figure 2A, right, shows the method used to determine calcium responses on a summed fluorescence image of ganglion cells expressing G-CaMP3. To determine the time course of G-CaMP3 fluorescence, we measured mean fluorescence intensity within oval ROIs at the selected ganglion cell soma (Fig. 2A, white oval at right).
To separate the effects of visual stimulation from activation by the imaging field, we kept the 488 imaging field constant and measured the calcium response to a brief pulse of UV light. Figure 2B shows the time course of the calcium response to a 1-s pulse of UV light, measured from the ganglion cell soma within the white oval ROI at right. The fluorescence intensity (Fig. 2B, red curve) rises slowly, peaking at ∼2 s and returning to baseline ∼4 s after stimulus onset.
Calcium responses to UV stimuli of longer duration.
Following the above measurement of responses to brief stimuli, the remaining data in this study were collected using an 8-s presentation of the UV stimulus, to reveal both transient and sustained calcium responses. Figure 3 shows examples of fluorescent responses to UV stimuli measured on day 6 following rabies injections. The temporal profile of responses varied across cells, increasing in some and decreasing in others. For cells having increased responses, the responses of some cells continued to increase or plateaued as long as the UV stimulus was present (e.g., Fig. 3, A, top, and B, top row), while responses of other cells peaked early during the 8-s UV stimulus and then declined (e.g., Fig. 3B, 1st, 3rd, and 4th panels from left in the 2nd row). For all cells with a decreased response, responses generally continued to decrease as long as the UV stimulus was present. However, after UV stimulus offset, the response of some cells recovered to baseline level rapidly (e.g., Fig. 3A, bottom, and Fig. 3B, middle in the 3rd row and the 1st panel from left in the 4th row), while the response of other cells recovered more slowly (e.g., Fig. 3B, 1st and 3rd panels from left in the 3rd row). To estimate the main peak response amplitude across ganglion cell population, we quantified peak responses of 60 imaged cells measured on day 6 (see appendix). Eleven out of the 60 cells showed statistically significant increase in fluorescence; 41 out of the 60 cells showed statistically significant decrease in fluorescence. The mean amplitude of increased fluorescence was 0.4 ± 0.4 (SD), which was approximately threefold greater than that of decreased fluorescence: 0.15 ± 0.04 (SD).
Calcium responses of ganglion cells across time after rabies injection.
To evaluate the value of FACILE methodology for chronic study of neuronal responses, we examined the calcium response of individual ganglion cells across multiple days during which the cells degenerated. Figure 4A shows repeated imaging on days 4, 6, 8, and 10 of two clusters of G-CaMP3 expressing ganglion cells in the retinas of two different mice. The total number of transduced ganglion cells was highest around day 6 (Fig. 4A, bottom). To assess the consistency of cell responses over multiple days, normalized responses of selected cells were shown in Figure 4B (see Fig. 3 also, for cells identified with same numbers). A transduced ganglion cell was typically visible for at least three of the time points, and different cells initially became visible on different days. For example, cells 1–4 were visible on days 4–8, whereas cell 5 was visible on days 6–10 (Fig. 4A). For cells showing increased fluorescence to visual stimulation, responses were typically stable for two time points and then greatly decreased at the third time point (e.g., cells 1–3 in Fig. 4B), whereas those showing decreased fluorescence often remained relatively stable until they could no longer be imaged (cells 7–12 in Fig. 4B).
This study demonstrated a novel method for the repeated in vivo imaging of calcium responses of individual retinal ganglion cells in the mouse eye. It utilized an AO imaging system (Geng et al. 2012; Gray et al. 2006, 2008) to obtain high-resolution images of fluorescing cells in retina despite the challenges of low fluorescence levels and eye movements. We employed an UV light source that activates the S-cone opsin to measure ganglion cell responses in the presence of the blue imaging laser, which also excites M-cone opsin and rhodopsin. Correction of optical aberrations by the AO system permitted imaging of the subcellular structure (soma, proximal dendrites) of ganglion cells (Fig. 2, A and B, right). Finally, the level of both imaging and stimulation light were kept low to avoid light damage that would compromise the repeated imaging of cells. This is the first report of high-resolution in vivo imaging of the light responses of individual retinal ganglion cells and shows imaging of calcium responses of ganglion cells at temporal and spatial resolutions comparable to those used in similar in vitro studies (Borghuis et al. 2011; Briggman and Euler 2011; Weitz et al. 2013; Zariwala et al. 2012). As described above, important advantages of the FACILE method are simultaneous, high-resolution in vivo study of numerous cells, repeated imaging of single retinal cells over a long time, and the ability to relate the physiology of recorded cells to their morphology. The high axial resolution of FACILE can distinguish RGC cell types on the basis of their branching level within the inner plexiform layer (Geng et al. 2012). A major limitation of FACILE imaging of calcium is poor temporal resolution compared with electrophysiology. However, in the future, we could avoid the inherently slow calcium response by using voltage-sensitive indicators (Mutoh et al. 2012). Other limitations of the present method can be reduced by incorporating methodologies described below in future directions.
Comparison with prior in vitro calcium imaging studies of RGCs.
The calcium response of ganglion cells to light stimulation has previously been recorded with in vitro preparations (Borghuis et al. 2011; Briggman and Euler 2011; Zariwala et al. 2012). These studies used either a calcium indicator dye, Oregon Green BAPTA, or a genetically encoded calcium indicator, G-CaMP3, imaged with a two-photon laser. Briggman and Euler (2011) studied the calcium response of mouse ganglion cells to a 1.5-s presentation of a 420- and 580-nm spot that ranged in diameter from 50 to 800 μm. Responses to a 800-μm spot (their Fig. 2A), the condition most similar to that used in the present study, showed increased or decreased fluorescent response similar to that seen in Fig. 3 in the present study. Borghuis et al. (2011) studied the calcium responses of mouse ganglion cell to a 2-s presentation of a full-field 458-nm stimulus (their Fig. 6B) and also showed increased or decreased responses to light in some cells similar to that seen in Fig. 3 of this study.
Both Borghuis et al. (2011) and Briggman and Euler (2011) showed cells with clear OFF responses, i.e., fluorescence increase above baseline level to light off. While many cells in our study showed decreased responses during the 8-s UV flash presentation (Fig. 3), none showed increased fluorescence above baseline after the 8-s UV flash was extinguished. The ∼24° UV spot used in our study was much larger than the optimal 10° spot diameter for mouse ganglion cells (Schmucker and Schaeffel 2004; Stone and Pinto 1993), which can greatly reduce OFF responses of OFF-center ganglion cells (Sagdullaev and McCall 2005). Thus, although our responses appear consistent with those reported by Briggman and Euler (2011) for spot diameter of 800 μm, smaller spot sizes will be needed to demonstrate unequivocal OFF responses.
Future directions for FACILE.
The FACILE method described here can be extended to additional applications by slight changes in calcium indicators, visual stimuli, imaging methods, and even different species. Long-term retinal changes could be examined over weeks to months by the use of less damaging methods for inserting GECIs into retinal cells than the rabies virus used here. Precisely focused patterned stimuli, generated with the AO system, can be used to map receptive fields with high precision. The method is currently being extended to nonhuman primates, which offer an animal model of retinal function and dysfunction that is closely related to human retina. Different imaging modalities will greatly extend the capabilities of the FACILE method. For example, the use of fluorescence resonance energy transfer (FRET)-based sensors can provide both ratiometric calcium imaging and structural imaging and extend the method towards studying fine scale molecular interactions. Furthermore, multiphoton methods (Hunter et al. 2011) will permit noninvasive FACILE imaging of many compounds involved in the retinoid cycle, thus potentially extending the approach to the human retina.
This work was supported by National Eye Institute Research Grants EY-019375, EY-021166, EY-004367, BRP-EY-014375, and NDC-5PN2EY-018241; National Eye Institute Training Grant EY-07125; and National Eye Institute Core Grant-EY-001319.
D. R. Williams has license agreements for AO technology with Canon and Physical Sciences and consults for GlaxoSmithKelin and Pfizer. He also has a Small Business Innovation Research grant on development of novel AO technology with Polgenix. All other authors declare no competing financial interests.
Author contributions: L.Y., E.M.C., D.R.W., and W.H.M. conception and design of research; L.Y., Y.G., F.O., R.S., A.H.C., and W.H.M. performed experiments; L.Y., D.R.W., and W.H.M. analyzed data; L.Y., D.R.W., and W.H.M. interpreted results of experiments; L.Y. and W.H.M. prepared figures; L.Y., D.R.W., and W.H.M. drafted manuscript; L.Y., Y.G., F.O., R.S., A.H.C., E.M.C., D.R.W., and W.H.M. edited and revised manuscript; L.Y., Y.G., F.O., R.S., A.H.C., E.M.C., D.R.W., and W.H.M. approved final version of manuscript.
We thank Jennifer Strazzeri and Stacy Ruvio for assistance with surgery; Jennifer Norris for assistance with data processing; William Fischer, Rachel Hollar, and Lee Anne Schery for assistance with cSLO or AO imaging; Kamran Ahmad for assistance with imaging software programming and hardware interface; Jennifer Hunter for assistance with light safety calculations; and Tracy Bubel for assistance with histology. Loren Looger supplied the G-CaMP3 plasmid and provided advice on its use.
Quantification of calcium responses across the RGC population.
On day 6 after rabies injection, we imaged the calcium responses of total 60 transduced cells from 6 retinal locations in 5 mouse eyes. Figure A1 summarizes the measured peak response (F/F0) and time to peak across population.
- Copyright © 2013 the American Physiological Society


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Written on: 18/11/2012
Looks to me like you have a rogue one there
the dealers have told me Kimco have a better quality control system in place which is the envy of other chinese scooter manufacturers and on par with the european brands now being produced in china
I am glad i have read your review before making up my mind
Written on: 10/11/2013
I have a 2009 and its a challenge to start up as well. The mechanic calls it very cold natured...I think its a pain and would never purchase another one.
Written on: 29/03/2012
my dad bought me mine for £850 and it seemed ok at first although it was a bit sluggish. it runs much better on hot days than cold and its top speed was 38mph. then i replaced the spark plug and oil and it now does 35-36mph which is strange according to all my friends. was de-restricted with a variator kit when it was bought so not sure how fast it was before. i like that it has the backrest because it is good support on long journeys which is good although it is not good on hills because i… Read Full Review
Written on: 04/01/2012 by james550 (1 review written)
i have recently got a kymco agility and was wondering if i was to add a 80cc bore kit would it make much of a difference to the speed ?… Read Full Review
Written on: 22/03/2012
will give you a top speed of 42mph with good acceleration, even in strong wind.
Written on: 28/04/2011
Right done my cbt on this moped and found it very nippy with plenty of acceleration, but restriction is a problem on lo jorneys, killer.
got ot home after my first jorney on this 09 bike and woossh! there goes the key, snapped in the ignition, this was a real bother with time and money that i didnt need at the time.
so the company need to make stronger keys otherwise just be gentle ;)
anyone else experinced this problem?… Read Full Review
Written on: 04/01/2012
strongly disagree i use mine for opening paint cans and doing other stuff your just heavy handed and you shouldnt judge a moped on the strength of its key
Written on: 09/01/2011
I bought my Kymco Agility 50 in October 2010. It is my first ever scooter and being a 25 year old female, I am probably not the typical scooter rider!
It cost me £1300 on the road. It was the cheapest scooter I could find with such good reviews. The dealer I bought it from was super helpful and the bike was all set to go after I completed my CBT.
I noticed at first that it is a little high. I am 5"4 and I can't put both feet down together because of my short legs. But once I got used to it… Read Full Review
Written on: 28/11/2010
I have had this bike for a month and within that time it has proved itself to be a well built, reliable and sturdy machine.
Bought directly from the dealer and although the restriction is in place got a steady, decent speed with a good quiet engine.
After owning a previous scooter which wasn't as well built as this, I am extremely satisfied with my purchase and would strongly recommend the Agility… Read Full Review
Written on: 06/05/2010
I bought the Kymco Agility 50 for £1000 on the road about 3 months ago. It's the latest model with the front indicators built in to the front fairing. I am a 6'1" 13 stone man and the seating position fits me well even though I can't put the back rest up and feel comfortable. The front light on the scooter is amazingly bright and performs well in even total darkness.
I'm just using it to travel 5 miles to work each day and its performing well. I get about 80mpg with it running flat out at 31mph… Read Full Review
Written on: 26/03/2010
I have had my 2009 Kymco Agility 50 a little over a week now, for my first moped and I like it so far,I haven't had any problems except trying to start it when it's cold out but when it's warm out it starts without a problem.If you need a moped just to get around town I reccomend the Kymco Agility 50 it's a great moped for someone who is a first time rider of a moped like I am and for the price… Read Full Review
Written on: 24/10/2009
it is good byke… Read Full Review
Written on: 20/10/2009 by granty3000 (1 review written)
hi i have a kymco agility 50cc 4 stroke i have had it for about 3 weeks i have brought it off ebay for £250.when i got it he had brought a new battrey for it because he said that the other one is dead. when i got home i put the battrey on charge for about 10 hours.i put it on the bike n i tryed to start it but it wouldnt start.so i go get the jump leads and i link them up with the car then put it the the scooter.then i start the scooter then it firers. so then i let it warm up then i turn it… Read Full Review
Written on: 28/09/2009
I have the 08 reg version of the Kymco Agility 50 and i think that it is a fatastic scooter 0-40mph in about 30 seconds the pick up at the traffic lights is fab and yeah its let me down a couple times but i have done 8,000 miles in the 6 months i have owned it and i cant really fault it. There isnt alot of space in the storage trunk under the seat though but im buying a top box which will happily accomidate all my stuff all in all if you ever want or are getting a moped get a kymco agility as… Read Full Review
Written on: 09/06/2009
400 miles on my new Agility 50; MPG about 90. Bike accelerates well, stops well. Will do 40 MPH on flats easily. Warranty from Kymco and dealer service is outstanding (have collected on warranty when gas tank float fell off). Performs well on any surface from pavement to sandy two-tracks. Breaking in like I mean to drive it and its performing well. Comfortable ride considering its low weight. Can store a full face helmet or a twelve pack of longnecks under the seat.
When I buy my 150… Read Full Review
Written on: 08/04/2009
The Kymco Agility 50 is very realiable never had a problem with it after 10kmiles so i cant fault it.. Only hink that went wrong was one of the indicators stopped working, but had it fixed in a day… Read Full Review
Written on: 13/01/2009
this bike can easily keep up with 2 stroke 50 cc bikes and is often better at accelerating. Its an awesome ride.
great acceleration for a 4 stroke
economical tyres slippy in the wet
the front doesnt look too tasty… Read Full Review
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text:
Fines, debt and money
Are you in debt, considering bankruptcy or does someone owe you money? Do you have unpaid fines?
liac resource legend
External link
fines debit and money resource tiles
This chapter of the Law Handbook deals with social security payments, including pensions, allowances and payments, and appeals.
This chapter of the Law handbook discusses superannuation, including superannuation funds, regulation, benefits, taxation, superannuation guarantee contributions, and dispute resolution.
This chapter of the Law handbook deals with income tax, including tax returns, PAYG tax, tax file numbers, deductions, capital gains tax, fringe benefits tax, and the GST.
This chapter of the Law handbook deals with bank accounts, electronic funds transfer, codes of practice, and disputes.
This chapter of the Law handbook discusses alternatives to bankruptcy, entering bankruptcy, the effects of bankruptcy, obligations, restrictions and rights, and ending bankruptcy.
This chapter of the Law Handbook gives an overview of consumer credit law, including responsible lending, fees and interest, disputes, pawnbrokers, and debt collection.
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text:
Catius, Titus
M. T. Griffin
Christopher Rowe
Cebes of *Thebes (1), like his compatriot *Simmias (1), is a main character in *Plato (1)'s Phaedo; he probably also shared with him long-standing membership of the Socratic circle (see Cri. 45b; Xen. Mem. 1. 2. 49, 3. 11. 17; see
Celsus, Roman author of The True Doctrine, late 2nd cent. CE
William David Ross and David Potter
Christopher Pelling
Chamaeleon, of *Heraclea (3) Pontica (c.350–after 281
Gisela Striker
Charmadas (168/7–some time after 107
Chrysippus, of Soli in Cilicia, c. 280–207 BCE
Julia Annas and Malcolm Schofield
Claudius Aristocles, Tiberius
M. B. Trapp
Tiberius Claudius Aristocles of *Pergamum (2nd cent.
Cleanthes, of Assos, 331–232 BCE
Julia Annas and Malcolm Schofield
Student of *Zeno (2) and his successor as head of the Stoa (see


text:
This product is no longer available.
You can try these similar gifts instead.
Skull Punch Bowl Set
We have a bone to pick. Why can't Halloween be for grown-ups, too? Enter this set that will help you kill it at your next Halloween bash. Punch bowl, six cups, and ladle included.


text:
The summer of Ilya Kovalchuk
September, 7, 2010
By E.J. Hradek
After 65 days (and a roughly 15 additional hours), Ilya Kovalchuk can finally settle into life in northern New Jersey as a true member of the New Jersey Devils.
For his part, stoic Devils GM Lou Lamoriello figures it was all just business. "I really have no thoughts about it," Lamoriello said on Labor Day. "It's behind us now."
Maybe it's behind you Lou, but I'm still thinking about it.
So, as I sit here at my laptop wondering where another summer went -- I'm already writing ESPN The Magazine NHL preview stories -- I need a little closure. I just have to get it out of my system. To that end, I took out my calendar and I tried to sketch the road traveled in the summer of Kovy.
I hope I didn't miss anything (although I probably did).


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I had no idea what to expect when I was asked to checked out the satin & silk fetish galleries. It took me about six seconds of browsing the pictures for memories of staring at catalogue underwear models to flood my head. Remember those sexy, seductive women posing in long, silky nightgowns that fell perfectly on their beautiful bodies? Those were the days when you depended on flyers from your local department store as your source for softcore. Remember how you used to wish you could slide of the models' satin clothing to see what lies beneath? In these pictures, the babes do just that.
All Rights Reserved © 2005-2017, FetishFish.com design and reviews are copyrighted.


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Written on: 18/06/2012 by MummaJones (1 review written)
Wonderful review website. Very easy to navigate. I have a dog and a young family and the website allows me to see very quickly if both are welcome at certain locations.
I will definatly be using this website in future and recommending to friends and family.


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LYONS, MARY Swartz Creek
Mary Lyons, age 81, of Swartz Creek, died September 1, 2013. Funeral services will be held 12 Noon Wednesday, September 4 at Sharp Funeral Homes, Miller Road Chapel, 8138 Miller Road, Swartz Creek, Pastor Michael Brown officiating. Interment 2 PM Wednesday at Great Lakes National Cemetery, Holly. Visitation will be held 1-4 and 6-8 PM Tuesday, 9 AM - 12 Noon Wednesday. Mary was born July 2, 1932 in Flint the daughter of Anthony and Frances (Reno) Salerno. She was married to Gene Lyons on September 30, 1958. She loved gardening, cooking, and canning. Surviving are: husband of 54 years, Gene; 5 children, John (Donna) of Swartz Creek, Frances (Jeff) Matherly of Beaumont, TX, Anita Miller of Swartz Creek, Gene Jr. (Nancy) of Grand Haven, MI, Tony (Amy) of Rockford, MI; 15 grandchildren; 5 great-grandchildren; sister, Anita (Albert "Chuck") Patella of Ohio; half brother, Vito Salerno; sister-in-law, Mary Salerno; many nieces and nephews. Mary was preceded in death by her parents; sister, Annette (Joe) Scaffidi; brother, Ignazio "Chuck" Salerno. Mary's family extends a special thank you to Caretel of Linden for their care and kindness. Tributes may be shared on the obituaries page of www.sharpfuneralhomes.com
The My Memorials Facebook app allows you to:


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WICHITA FALLS — The contempt charge against Dallas County District Attorney Craig Watkins now rests in the hands of a Republican judge known here as a hard-nosed but fair operator.
Outside the courtroom, Bob Brotherton is a fan of fast cars, hot sports and hunting.
"Judge Brotherton is well-respected, fair and intelligent," Wichita County District Attorney Maureen Shelton said. "He's very professional and runs an orderly court."
Attention has now fallen on Brotherton, 62, who has been a state district judge since 1989, after being picked to hear the case against Watkins, a Democrat.
Watkins was held in contempt by state District Judge Lena Levario in Dallas for refusing to testify at a hearing looking into allegations of prosecutorial misconduct. If Brotherton upholds the charge, Levario can then decide the punishment.
Brotherton has declined to talk to reporters other than providing a copy of his résumé and a general framework of the upcoming hearing.
Those who know him and have appeared in his court say that outsiders should expect the proceedings to be run strictly by the book — perhaps leavened by a splash of humor.
Edward Lane, a lawyer and publisher of the online Wichita Falls Law Enforcement Examiner, said Brotherton has a reputation for being "very fair."
Brotherton was prosecutor in the early 1980s in Wichita County, giving him experience "on both sides of the bench," Lane said.
He also said the judge "has a good sense of humor, in and out of the courtroom."
In his judicial website photo, he appears in black robes, smiling, with wire-rimmed glasses and a graying mustache.
During his tenure, Brotherton has handled several high-profile cases, according to the online publication that listed some of his rulings. Among them:
This month, he sentenced a Wichita County man to 40 years in prison for child sexual abuse and 20 years for aggravated sexual assault in a case that involved the abuse of two girls, ages 8 and 5, while their mother was at work. Both sentences will run concurrently, and the man has no possibility of parole.
In February, Brotherton sentenced a Wichita Falls day care operator to the maximum two years in state jail after she pleaded guilty to drunken driving with three toddlers in her van. Her attorney had sought probation.
He gave a local man with a prior assault conviction eight years in prison for slashing his wife with a knife during an argument last year. The husband was mad because she brought home the wrong kind of pizza.
He also sentenced a Wichita Falls father to prison for life without parole for killing his 23-month-old son with a claw hammer.
Lane, who has been a prosecutor before Brotherton, said the judge also is known for his love of hunting and NASCAR.
"He has been to a bunch of races," and is a fan of Jeff Gordon, Lane said. He also was a friend of the late Lloyd Ruby, a champion driver from Wichita Falls.
Sports runs in the family.
Lane, a classmate of Brotherton's at Rider High School in Wichita Falls, said he was an outstanding tennis player there.
The judge's father, Paul P. Brotherton, was a minor-league baseball player. His parents moved to the area in the 1940s so Paul Brotherton could play for the Wichita Falls Spudders.
Brotherton is a past volunteer and former participant in the Hotter'N Hell Hundred, the popular bicycle ride held each August in Wichita Falls.
After high school, he earned an undergraduate degree in marketing from the University of North Texas, and graduated from the University of Texas Law School in 1979. He was a lawyer in private practice before becoming a municipal court judge and later a district judge.
"He's a fantastic person," said Donna Long, head of the Wichita County Republican Party. She said he and his wife, Mitizi, a court administrator, are well respected and active in the community.
Brotherton ran unopposed for office last time in the Republican-heavy county, and he faces re-election in 2014.
"He's a good choice for the case in Dallas," Long said.


text:
How do I return to the original iTunes store of my country from that of a foreign one?
I had to change to the Italian itunes store to purchase something and now I need to go back to the Maltese one because I cant neither puchase nor update applications.
- Asked by Rosanne S
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text:
Panthers' quarterback Cam Newton made it clear that he wasn't happy following the team's 19-14 home loss to the Dallas Cowboys on Sunday. It was Carolina's fourth loss in a row and their fifth on the season, dropping them to a woeful 1-5 record. It's not that Cam & co. were blown out on Sunday--rather, Newton has become frustrated with the team's ability to keep games close, paired with their inability to pull out victories.
Not counting the 36-7 drubbing at the hands of the Giants in week three, the Panthers have lost four games by a combined 17 points.
"This taste, this vibe--I'm not buying it, man," Newton said afterward, per Yahoo! Sports. "And I don't know what it is, but something's going to have to change. Something's going to have to change real fast. … We just find a way to keep the game close just to see what happens at the end. I'm getting tired of it, and that's not a formula to win. Domination is a formula to win."
For what it's worth, the Panthers did make a change after the loss to Dallas, firing general manager Marty Hurney on Monday. While we kind of doubt that, as some have suggested, the move was made in response to Newton's remarks, we'll see if it makes any noticeable difference going forward--or if Newton will be left to continue airing his grievances to the eager ears of the media as he did on Sunday.
"I'm going to bring in a suggestion box," Newton said. "And I want your suggestions in that suggestion box, because I sure don't know. I really don't."
Stay on top of the latest Dallas Cowboys news with NBCDFW.com. Follow NBC DFW on Facebook or have breaking Cowboys news sent directly to your phone with Blue Star SMS alerts. Text "bluestar" to 622339 (NBCDFW) to subscribe, text HELP for more info, and text STOP to end the subscription. Message and data rates may apply.Read more about it here.


text:
Chelsea rocked by Terry injury blow
Chelsea captain John Terry will miss Tuesday's Champions League tie at Napoli amid reports he could be out for two months with an ongoing knee problem.
• Cavani: We'll end English hopes
Chelsea were hoping to have Terry back for the daunting trip to the Stadio San Paolo and the centre-back pushed himself through a pre-match training session on Monday, despite clearly struggling.
According to the Daily Mirror, Terry woke in agony at the team hotel as his knee problem worsened overnight, instantly ruling him out of contention for a game that could define manager Andre Villas-Boas' reign.
Chelsea have failed to keep a clean sheet in Terry's absence and needed a defensive boost as they prepare to face Napoli's highly-rated attacking trio of Ezequiel Lavezzi, Marek Hamsik and Edinson Cavani.
Terry has missed the last four games after being forced to give way to the knee injury he sustained when he collided with the post making a goalline clearance in the FA Cup win over Portsmouth on January 8.
It was hoped that rest and recuperation would cure the problem of bruising on the bone, which Terry had initially played through, but the defender will now undergo what was described as a "small procedure" by club medics when Chelsea return to London.
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text: Should get there in 2 months. Started the Christmas cheese today. 5# colby 5# mild cheddar and a few pieces of extra sharp. Trying these with peach, I haven't done peach before but there and so many rave reviews here on the site I'm giving it a shot. I normally use hickory or apple. Outside temp is 63 this afternoon so I shouldn't have much issue with heat. I go 4 hours with smoke then let rest a little then vac packed for at least 2 months. The little bit of sharp that I have left in the fridge is from march. Most of this is for Christmas presents so it will get a 2 month rest. Should be really good. John


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Finally it's here, the 1st event for the club, The Mass Firebirds spring kick-off get together. Our club will met for breakfast at 830am, Denny's 111 Enfield st., Enfield Ct. then cruise (approx. 1hr) to the event in Spencer Ma.. If you would like to just cruise with the club we will depart Denny's by 10am.
Route to Spencer Ma.
https://www.google.com/maps/dir/133+W+M ... 208566!3e0


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by Alyssa Oursler | August 3, 2012 6:30 am
There's going out of fashion, then there's getting thrown off the runway — and it's the latter that has happened to apparel retailer Abercrombie & Fitch (NYSE:ANF[1]).
ANF — which is made up of Abercrombie & Fitch, A&F Kids and Hollister Co. stores — was a perpetual growth story coming out of its 1996 IPO, going from around $10 per share to above $80 before the 2008-09 crisis. Like almost everyone else, Abercrombie took its dive, but it has soared from its crisis lows, roughly quadrupling by late 2011.
But things have gone from good to bad pretty quickly.
In a battle to bolster profitability, Abercrombie closed around 130 of its namesake locations in the past couple years. Then in June, amid shrinking earnings and after a roughly 60% slide from its 2011 highs, it announced plans to close around 185[2] by 2015.
Thing went from bad to worse Thursday, when ANF said its second-quarter earnings — to be reported Aug. 15 — would come in between 15 and 18 cents per share[3], or about half of what Wall Street analysts were forecasting. Investors battered shares by about 14%, erasing the gains Abercrombie had made since bottoming in June.
Considering ANF's rapid movement, you can be excused for being left a little dazed. So let's look at just how we got here.
To start, if Abercrombie & Fitch thought it could avoid the world's economic struggles by dimming the lights and hiding behind half-naked mannequins, it was wrong.
Europe has hit many U.S. companies hard, and A&F is no exception. The company gets about 20% of its sales from Europe, and the market has understandably dried up amid the eurozone's sputtering. Growth abroad originally helped spur ANF's revenue growth, but things have slowed down to say the least. In the most recent quarter, same-store sales dropped 26% outside the U.S.
However, the impact of increasingly cost-conscious consumers at home is weighing heavily on ANF's business, too. Shoppers are strapped for cash, and they're looking for deals wherever they can find them.
That means customers that usually would've been fine forking over $50 for an Abercrombie's hoodie now are considering the Aeropostale (NYSE:ARO[4]) version that's half the cost. The same price difference holds true for tanks and tees as well — Aeropostale generally has twofer deals that run around the same price as a single A&F item.
ANF does have a cheaper selection at its Hollister stores, but it has failed to differentiate the two brands enough to avoid having Hollister simply steal customers from Abercrombie. The only differences in the Hollister brand, besides slightly cheaper pricing, is that it tries to give off a more Californian vibe and switched out the moose logo for a seagull.
Even the approach of slashing prices to keep up doesn't solve much — it just means pressuring the company's already middle-of-the-road margins.
So, as I said above, the atmosphere Abercrombie has created doesn't prevent typical retailing woes. Actually, it makes things worse.
See, the store isn't meant to appeal to older shoppers[5] — and by "older," I just mean out of their mid-twenties (and even then, mid-twenties is pushing it). This is evident by the low lighting, loud music and overall wannabe nightclub feel of the store that most adults can't tolerate. Same goes for the small sizes and teenage-style apparel.
Compare it to competitors like American Eagle (NYSE:AEO[6]) or Aeropostale, and the differences are stark. Sure, they also target a younger audience, but they have friendlier, more family-oriented atmospheres (with no scantily clad models).
AE and Aero, for example, rarely if ever feature graphic tees that could be seen as racy or inappropriate, while ANF has faced countless boycott calls[7] for "selling sex" to children and for offensive graphic tee slogans.
Plus, ANF's competitors aren't so hostile to the slightly older customer that might stumble into their stores. At Aeropostale, for one, camisoles are a perfect example of an item with the ability to bring in customers outside of the teen demographic. They are sold in every color and size, are always part of some kind of sale and are a basic staple of most women's wardrobes.
Not that Aeropostale is without its own problems. The retailer still is pretty teen-oriented, and its own struggles with lower traffic[8] and flat same-store sales have led to lower guidance, too — and a subsequent Thursday flogging.
American Eagle, on the other hand, is by far the most mainstream and versatile of the three, and it shows. AEO has forecast stronger-than-expected sales[9] thanks to an increase in store traffic last semester.
The bottom line is that Abercrombie lacks the ability to attract customers — whether because of high prices or low lighting — and that tends to be an important quality in retail.
For the nail in the coffin, just look at the details of Abercrombie's aforementioned lowered forecast: A full-year earnings outlook of $2.50 to $2.75 per share — a whole dollar lower than previous forecasts and much lower than analyst calls for $3.35 — and same-store sales expected to drop in double digits for the year.
Between its off-putting atmosphere and overpriced apparel, it's clear that ANF is a fashion fad that has more than flopped. Even after its involuntary price cut, stay away from the stock — even if you feel the need to wander through one of its subpar stores.
As of writing this, Alyssa Oursler did not own a position in any of the aforementioned securities.
Source URL: https://investorplace.com/2012/08/abercrombie-a-downward-fashion-trend/
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text:
Plastic Surgeon On A Mission To Help Elephant Man-Like Deformity
(PR Newswire) Dr. Oleh Slupchynskyj, Director of The Aesthetic Institute of New York and New Jersey, has begun a humanitarian effort to help a Kenyan man afflicted with massive, painful facial tumors known as keloid scars (see images here). Due to his impoverished situation and the lack of qualified medical care for this condition in Kenya, this patient has been unable to find help. Late last year, he reached out to Dr. Slupchynskyj, a Manhattan-based facial plastic and reconstructive surgeon with extensive experience in correcting facial and body deformities resulting from keloids.
Dr. Slupchynskyj reports, "Keloids are tumor-like scars growing out of the regular confines of a skin injury. They can grow to sizes where they are extremely debilitating to a patient both physically and emotionally." Keloid scars predominantly affect patients of African descent but are also seen in patients of Indian and Asian descent, but less commonly in Caucasians. Keloids can become chronically infected, cause pain and persistent bleeding. "In rare instances, keloids can degenerate into malignancies and their appearance can cause significant psychological stress and social stigma. I've seen it first-hand," states Dr. Slupchynskyj. This patient has been unable to provide for his family due to the ongoing debilitation caused by his scars. "Unfortunately, few surgeons are experienced in treating keloid scars and/or are willing to tackle the issue," continued Dr. Slupchynskyj.
Dr. Slupchynskyj has agreed to treat this patient free of charge. However, due to additional expenses associated with his care, including travel, lodging and some hospital facility fees, the doctor is reaching out to others who can provide financial assistance to help bring this patient here and make his surgery a reality. Interested donors can contact the Patient Care Coordinator Cara at firstname.lastname@example.com.


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This website is scheduled to undergo routine maintenance on Saturday, 18 November from 11:00 GMT - Sunday, 19 November 05:00 GMT. During this time, you may not be able to schedule, reschedule or cancel test appointments. We apologise for this inconvenience and thank you for your patience.
C++ Institute Certifications
C++ Institute was established with a purpose to promote the C/C++ programming languages, provide a global framework for C/C++ examinations as well as enhance, develop and support professional careers in C/C++ programming and related technologies.
C++ Institute is proud to offer professional certifications designed to provide industry recognition of your knowledge, skills and proficiency in C and C++ programming.
C++ Institute built two paths of education, testing and certification. The first path covers the C programming language and the second one the C++ programming language. Each programming path consists of three levels of qualifications: associate, professional, and senior.
The growing demand for C and C++ programmers has resulted in the necessity for gaining recognition for one's programming skills and expertise. C++ Institute and Pearson VUE have undertaken to provide exclusive delivery of the certification program they have created for the C and C++ programming languages.
Find the right exam
C++ Institute is happy to offer the following certification exams to test candidates:
|CPA – C++ Certified Associate Programmer
|65 minutes (exam) + 10 minutes (NDA/Tutorial)
|CPP – C++ Certified Professional Programmer
|65 minutes (exam) + 10 minutes (NDA/Tutorial)
|CLA – C Programming Language Certified Associate
|65 minutes (exam) + 10 minutes (NDA/Tutorial)
|CLP – C Certified Professional Programmer
|65 minutes (exam) + 10 minutes (NDA/Tutorial)
To gain a higher level certification the candidate must already hold a lower level credential. This means that if a candidate wishes to receive a CPP certificate, they must pass two exams: CPA and CPP. Similarly, if a test candidate wishes to receive a CPS certificate, they must pass the CPA, CPP and CPS exams. The same applies to the C language path.
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C++ Institute exam vouchers are available for purchase through C++ Institute authorized partners or via C++ Institute Voucher Order form. If you have any questions about ordering vouchers, you are welcome to contact C++ Institute at email@example.com.
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You can sign up for C++ Institute's online courses to get prepared for the CPA, CPP and CLA certification exams delivered at Pearson VUE Test Centers. The courses are free of charge. Completing a given course will entitle you to a discount voucher on your exam. To register for C++ Institute's courses, please visit C++ Institute C/C++ Education Platform. To find more study resources, please go to www.cppinstitute.org.
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C++ Institute has created an online system to verify all C++ Institute credentials held by candidates all over the world. Candidates may now have others easily verify their certification status by using a unique certificate verification code. The certificate verification code can be found on all certificates issued by C++ Institute in the bottom right-hand corner.
If you wish to authenticate a certificate issued to an individual, visit our Certificate Verification page and enter the candidate's verification code for each certification they hold. After entering the verification code, you will be presented with authentication details relating to the holder of the certificate.
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When a candidate fails an exam he or she must wait 15 days before being allowed to re-sit that exam. There is no limit to the number of retakes of a failed exam. No retakes of a passed exam are allowed.
Certification News:
C++ Institute is pleased to announce the redesign of the CLA – C Programming Language Associate (CLA) exam. The new version of the CLA exam (2.0) published on November 5, 2015, replaces the old version of the exam (1.0).
C++ Institute is pleased to announce the design of the CLP – C Certified Professional Programmer (CLP) exam. The new exam is planned for publishing in December 2016. Free CLP courses will be made available via C/C++ Institute Education Platform to all testing candidates who hold the CLA certification.
C++ Institute is pleased to announce that all candidates who hold the CPA certification can access the CPP course via C/C++ Institute Education Platform free of charge. The CPP course prepares candidates for the CPP – C++ Certified Professional Programmer certification exam.
Free second shot! Each candidate who has paid the full exam price (USD 295) or has completed the CPA/CPP/CLA course in the self-study mode (50% discount voucher) in the C/C++ Education Platform is eligible for a free retake of the CPA/CPP/CLA exam. A candidate who meets the above criteria and wants to receive a free voucher to re-sit a failed exam must write an email to firstname.lastname@example.com no later than 30 days of failing the exam, and include in the message the following details:
- text in the subject line of an email: CPA exam – retake voucher request (or CLA/CPP exam – retake voucher request if the candidate requests a voucher for the CLA/CPP exam),
- information in the body of an email: CPI ID (C++ Institute Testing ID), date of failed exam (day-month-year), full name, email address linked to the candidate's Pearson VUE account.
A voucher that entitles the candidate to a free retake of exam will be sent within 7 days of sending the request. The voucher will be valid for at least one month.


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Tel:++62 361 286 283
Fax: ++62 361 286 284
U.S.A. Fax:(toll free) 1-800-506-8633
U.K. Fax: ++44-20-7000-1235
Australian Fax: ++61-2-94750419
24h: ++62 812 3819724
Following the sentencing of a Javanese laborer, Mawardi, to 20 years in prison for his central role in the murder of Japanese tourist Hiromi Shimada on Christmas Day 2009 [See: Man Who Killed Japanese Woman Gets 20 Year Sentence], his accomplice in the homicide, Abdurrahman (21), has been sentenced to 10 years of prison.According to Beritabali.com, the sentence was handed down by the Denpasar District Court of Thursday, September 23, 2010, and read by the lead judge Putu Suika. The sentence was three years less than the 13 years requested by prosecutors.Judge Suika, in rendering his sentence, found the young laborer guilty of assisting in the murder of the Japanese woman and for trying to rape the woman. In handing down the lesser sentence, the judge said the polite demeanor of the accused and a clean criminal record prior to the murder warranted some degree of leniency. On the other hand, the judge said that weighing against the man's favor was the consideration that his criminal behavior had done considerable damage to Bali's reputation as a world tourism destination.Mawardi (31), the man found to have played the key role in the murder, was sentenced to 20 years imprisonment in a separate trial that ended in late August 2009.The two men robbed the Japanese woman, killed her and attempted to rape her at the time of her death.


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Is precognition a prescription for happiness or disaster....?
The good things in life are coming together for Kristina Collins. She's found her ideal home, her career is on track for mega success and the man of her dreams has finally come back into her life.
In Fate, the first installment of the Timeless Trilogy, Kris Collins discovers the benefits and risks of having precognitive visions while being stalked by a serial killer. Her friends can't help her, the FBI can't save her; she must save herself.
The Timeless Trilogy heroines, Kristina, Veronica and Cassandra, each deal with paranormal abilities as they discover and rediscover eternal love.
©2011 through 2111 Tallulah Grace (P)2013 Tallulah Grace
"Very entertaining paranormal drama!"
The narration is a little off for me but the the storyline is totally a worthy read!
"Awful "
The story was SO basic & read with all the inflections in the wrong places 😩 don't bother
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Most enjoyable puzzle; thanks, Suffolk
I love the story this painting tells! The new bonnet, the father with the bill looking sooo unhappy, the little sister taking it all in and perhaps a little jealous, the dog sniffing the shoes, the trap door and even the fireplace insert.


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While the city of St. Paul warns fossil hunters at Lilydale Regional Park about potentially hazardous and unsafe conditions in the park, some of the parents whose children were injured and killed in a May 22 landslide during a school field trip at the park say the St. Louis Park school didn't tell them about the dangers.
Four days after his 9-year-old son Haysem Sani was killed by a landslide, Mohamed Muse visited Lilydale Regional Park with his cell phone camera in hand.
"Look at here where my son is dying," says Muse. "This is a dangerous place. How you gonna bring kids here?"
The trail in Lilydale Park remains closed.
Haysem's parents, along with the parents of 10-year-old Mohamed Fofana, who was also killed, and 10-year-old Devin Meldahl, who was buried up to his hair but survived, all say they wish they had known more about Lilydale, about the bluffs and the steep, winding and rocky terrain.
"We trusted the school like our family," says Muse. "That's a very, very dangerous place. Why they allowed to go over there?"
A permit from the St. Paul Parks and Recreation Department is required to fossil hunt at Lilydale. The applicant has to sign a waiver, essentially agreeing that the city isn't responsible for anything that might happen.
The waiver specifically states that some of the "conditions and locations" in Lilydale are "hazardous," even "unsafe."
In April, a fourth grade teacher applied for the permit on behalf of Peter Hobart School in St. Louis Park and electronically signed the waiver, "certifying" and accepting "responsibility" for making the conditions of the permit and the waiver "known to all participants ... or in the case of minors, to their parents/guardians."
But the parents whose children were killed and injured say no one told them about the waiver or the hazards at the park.
In fact, parents say they signed a blanket field trip permission slip at the start of the school year. The slip applies to all field trips and does not mention anything about Lilydale, stating only that "teachers" will provide parents with "other relevant information" about each trip.
The school did provide some additional information in a flier given to students before the field trip.
Haysem Sani told his mother, Sartu Nagayo, about the field trip the night before, but she didn't see the flier until two weeks after his death, when she found it in his backpack.
"He said, 'We're going to go look for fossils.' That's the only thing I know," says Sartu.
The flier states that the students would be taking a "fossil-finding class" at Lilydale, mentions lunch and the need for sturdy shoes, but includes nothing about unsafe conditions at Lilydale. The field trip flier states that kids should be prepared for hiking, climbing and getting muddy. At the bottom of the page, the flier references the permission slip parents signed at the beginning of the year.
"I was going to play football this year," says Devin Meldahl, who was severely injured in the landslide. "I like to play football. I like to go outdoors and hunt and fish. I like to play basketball, baseball. I like to ride my bike."
Devin faces a long recovery. His mother Danielle says she saw the Lilydale flier before the field trip, but didn't think anything of it.
"It didn't say anything that stuck out to me that it was going to be not a safe place to go," says Danielle.
The district field trip policy in St. Louis Park states that field trips will be conducted in a "safe environment."
"You don't think that the school might bring them in a place that's not exactly safe or they'll notify you first," says Danielle.
Yet many schools go fossil hunting at Lilydale Park.
KARE 11 and Minnesota Public Radio News have learned that the parents of students at Buffalo Community Middle School were notified about potential hazards before their kids visited Lilydale that very same day. The permit waiver language was laid out in Buffalo's permission slip.
Danielle Meldahl wishes she had the same warning.
"Because I don't think I would have let Devin go," she says.
Lilydale is not unlike many wild nature areas in Minnesota. City officials describe it as largely natural and unmanaged.
St. Paul issues about 400 fossil hunting permits every year. Even Peter Hobart Elementary students had been there before without incident.
"It's a great experience for those kids," says parent Kasey Shipp.
Shipp's daughter Kylie was on this year's fossil hunting trip when the landslide happened. Her two older kids have been on the field trip as well. She says the school gives plenty of information ahead of time.
"There's no way that you would have been able to know that that was going to happen," says Shipp. "It's just a crazy accident."
It's something that could ultimately play a role in any potential legal fight. Minneapolis attorney Bob King is a veteran trial lawyer who once won a settlement from Roseville Schools after a student drowned on a field trip. He says winning a case like that can be difficult.
"If there is a lawsuit, it's going to turn on what a reasonably prudent school district would've, should've done," says King, who isn't representing any of the families from Peter Hobart.
The St. Louis Park school district turned down repeated requests from KARE 11 and Minnesota Public Radio News for an interview about what happened that day and what parents were told about the field trip. It said in a statement that it cannot provide any specific information about the incident, citing the privacy rights of the people involved.
The district did, however, answer some questions.
According to St. Louis Park Public Schools, there were 46 children on the field trip, supervised by two teachers, two paraprofessionals, 7 adult chaperones and a paleontology guide.
An adult who was there the day of the landslide told KARE 11 and Minnesota Public Radio News that the children were supervised at all times and not simply allowed to go anywhere they wanted in the park, including the kids who were injured and killed.
An attorney for the district denied our other data requests, citing the potential for a civil lawsuit, although no lawsuit has been filed. Some of the families, however, are considering their legal options.
The city of St. Paul hired an engineering firm to study the area after the landslide and then hired an attorney to conduct an independent investigation.
A fourth child, Lucas Lee, also suffered a broken ankle in the landslide. His parents say he is recovering physically, but is still struggling emotionally with what happened.


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Grow Your Business
Your Business Expansion Begins Here
Helping businesses achieve their goals is the backbone of the La Plata County Economic Development Alliance and the key to successful economic growth. If you're looking to grow your business or are considering a corporate relocation, we provide the tools to simplify the process. Through our strong network of dedicated individuals and organizations, we are your first stop for accessing the local, county and statewide information and resources to further your objectives. We can help with any question you have, whether it is about capital, permitting, local business services, government contracting, workforce recruitment or more. Our services even include small business advice through our extensive mentoring and consulting.
Take a more direct route to your next level of success by contacting us today.
Find your Workforce in Durango.
- Business Resources
- For Entrepreneurs
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- Site Selection 101
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Everything you need to know about growing your business is right here! Find out more about business planning assistance from the SBDC, loans and state programs from Region 9, networking and business training from the Chamber, and statewide programs that are offered to help with procurement, trade, contracting, and new market opportunities.
With a "think local" philosophy and outstanding resources, Durango, Colorado is truly an entrepreneurial mecca. At the Economic Alliance, we know difficult questions arise on how to open a business successfully. That's why we offer guidance and expertise that entrepreneurs need to feel confident in their direction. Buying or starting a business is a heroic feat and with proper support can lead to many future celebrations. Make us part of this exciting journey and together we'll turn your ideas into viable results.
Learn More
There are hundreds of events throughout the year to help with the challenges you face everyday in running your business. Whether you want to find out more about the local economy, meet with Alliance investors, attend conferences and workshops, network with fellow business owners, or go to seminars on specific topics, there is something happening almost every day to help you out. Find out more about upcoming events that might be relevant for you on the Alliance calendar.
In today's marketplace, attracting top talent can be challenging for any business. The Alliance stands ready to assist your firm in telling "the story" of La Plata County to your top prospects- not just the story of the great outdoors that so many people know and love, but about our top quality schools, friendly neighborhoods, health care, and arts and culture. The Alliance and its investors can also help integrate the whole family into the community by promoting professional, entrepreneurial, volunteer, and extra-curricular opportunities.
Having access to a talented workforce is the heart of a successful business. The resources here in La Plata County will make sure you can find, train, and bring in the best and brightest for your business needs. Home to Fort Lewis College, Southwest Colorado Community College and neighboring San Juan College in New Mexico, educational opportunities both for degree programs and continuing education are many.
Are you ready for finding new space for your business, but unsure of what to expect or look for in your search? The site planning process can seem confusing and difficult… but it doesn't have to be! The Alliance's "Site Planning 101" page can help you better understand where you are in your business planning process, and what you need to consider when thinking about leasing, buying, or building your next facility.
Outgrowing your old space? Searching for a new location closer to your home and workforce? La Plata County has a large region to accommodate different types of businesses. The Economic Development Alliance can assist you wherever you plan to have a business and the rules associated with each area and with each type of business. Whether you are thinking within the City limits of Durango, Bayfield or Ignacio, the Alliance can assist with finding the right location to meet your needs. Call us today to find out more about more about how we can assist!
The La Plata Economic Development Alliance works closely with the governing bodies in La Plata County to make sure that all businesses get the assistance they need in order to succeed here. Here you will find more information on the local governing bodies, how to contact them, and key information for buying, building, and permitting your business.
Contact us today to see how we can help you.


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At the advice of the Utah School Boards Association, the Morgan County School Board passed a new employee personal firearms policy. Although the district did not condone the use of concealed weapons in the classroom, it did say once they have drawn their weapon, they are responsible for their actions, Superintendent Ken Adams said. The school district is not liable. When you put that weapon on you, you accept the liability, said Robert Kilmer, human resource director. The policy reads: Except for designated school resource officers, possession or use of a firearm is not required for or part of the fulfillment of the job responsibilities of any employee of the Morgan County School District. Possession or use of firearms by district employees is therefore not requested by or endorsed by the district. Any and all liability or harm arising out of an employee's decision to possess or use a firearm will be the sole responsibility of that employee, without any recourse to or liability protection from or through the district. Adams said the policy was based on those in use in other local school districts.
District passes personal employee firearms policy
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Team Valor International is sponsoring the $75,000 Rushaway Stakes at Turfway Park March 23, and as fate would have it, it will also use the 1 1/16-mile Polytrack event as a last-ditch effort to get Crop Report to a prep for the Kentucky Derby Presented by Yum! Brands (gr. I).
Crop Report, a well-regarded 3-year-old son of Medaglia d'Oro out of 1998 Delaware Handicap (gr. III) winner Amarillo, got a late start to his U.S. racing career after two starts at Newmarket. He made his North American debut for trainer Graham Motion on the Gulfstream Park turf, running third in an allowance event there Feb. 23.
"It's been unbelievably frustrating with this horse," Team Valor CEO Barry Irwin remarked. "Very little has gone right. I tried to buy him after he broke his maiden, but the owner wouldn't sell him. I ran into the trainer down at Melbourne but by the time we reached a deal it had taken about five weeks, which cost us a lot of time so the horse got here late. It's been a slow, drawn-out thing."
The connections entered Crop Report in a dirt allowance at Gulfstream March 17, but scratched after the colt drew the outside post. His post for the Rushaway? Outside in a field of nine under 118 pounds including jockey Joel Rosario.
"What are you going to do?" Irwin said. "He's ready to run right now, we don't want to wait any more. Our only shot to make the Derby is to make the (April 13) Blue Grass (gr. I) and if we win this race we might make the Blue Grass. We probably won't have enough earnings, but we might. I don't think we're going to make the Derby with him, but maybe the (May 18) Preakness (gr. I). I also think the (June 1) Belmont (gr. I) would be awesome for him."
Team Valor won the Rushaway in 1995 with Key Guy and finished second in 2011 with Crimson China. Crop Report will be one of two runners getting blinkers for the 1 1/16-mile event; allowance winner Stormin Monarcho is the other.
"We worked him on the dirt with Cerro, who is a pretty nice horse, and Crop Report outworked him pretty good," Irwin said. "First on the dirt, first work with blinkers. With the blinkers, he paid attention. He's a very big horse, he has trouble leaving the gate with the other horses because he's just so damn big, but once he gets going his stride is tremendous. He's got a commanding presence with a long, sweeping stride, and he just gives you the impression there's a lot of power there."
Carrying high weight of 124 pounds in the Rushaway is Mark Casse trainee Star Contender, a son of Henny Hughes making his first start for owner John Oxley since a sixth in the Jan. 1 Gulfstream Park Derby. Casse also has G. G. Racing Stables' Honorable Guest, unraced since winning the Nov. 7 Frost King Stakes going seven furlongs on the Woodbine Polytrack.
Also of interest is Skychai Racing and Sand Dollar Stable's J. J. Got Even, a son of Stephen Got Even who is undefeated in two starts at Turfway. The colt won a one-mile allowance race by two lengths Feb. 15. Rosie Napravnik will ride as part of a 120-pound assignment.
Hall of Fame trainer D. Wayne Lukas will send Red Wings from his deep bench of 3-year-old contenders. The English Channel runner broke his maiden Jan. 26 going a mile at Oaklawn Park and came back to finish third in a 1 1/16-mile allowance Feb. 17 behind Carve and Title Contender.
Divine Ambition, Takin the Sloroad, and Bet a Bundle complete the field.
$75,000 Team Valor Rushaway Stakes (Race 8, approximate post 4:42 p.m. EDT), 3YOs, 1 1/16 miles (All Weather Track)
PP, Horse, Jockey, Weight, Trainer
1. Red Wings (KY), J.K. Court, 118, D W Lukas
2. Divine Ambition (KY), C.J. Lanerie, 118, D Miller
3. Takin the Sloroad (KY), T.L. Pompell, 118, G S Bush
4. Honorable Guest (ON), N. Arroyo, Jr., 122, M E Casse
5. J. J. Got Even (KY), R. Napravnik, 120, M J Maker
6. Star Contender (ON), M. Mena, 124, M E Casse
7. Stormin Monarcho (KY), S.B. Martinez, 118, J Berndt
8. Bet a Bundle (KY), A.T. Tamburello, 118, K Jarvis
9. Crop Report (KY), J. Rosario, 118, H G Motion
Blinkers On: Stormin Monarcho, Crop Report


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Role Link Shape
A Role Link shape contains placeholders for an implements role and a uses role. It can include one of either, or one of each.
You can add port types directly to a Role Link shape, using either existing roles or new roles, and existing or new port types.
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|Identifier property
|Type a unique identifier for this role link.
|Implements Role property
|Click the ellipsis (…) button and use the Role Link Wizard to specify the role that this orchestration will play in the role link.
|Initiating Role property
|From the drop-down list, select the role that initiates this role link.
|Ordered Delivery property
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|Report to Analyst property
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Wisdom from the Monastery: A Program of Spiritual Healing (Paperback)
Usually Ships in 1-5 Days
For almost two millennia, Christian men and women have banded together to live in devotion to God and humanity. Recent studies have shown that, on average, men and women in Holy Orders are healthier and live longer than the rest of us. What timeless solutions to the things that ail us might we have dismissed in our rush into the modern age?
Written by three very modern seekers who visited monastic communities to explore facets of the contemplative life, "Wisdom from the Monastery" reveals what these lay practitioners found so rewarding and deeply relevant to their lives today. Originally published in Germany as three separate volumes, the U.S. edition combines these major aspects of monastic practice: fasting, healing, and meditation. The book's warm, engaging tone presents millennia-tested practices of contemplative Christianity free from Church dogma. One need not be Catholic, nor even Christian, to benefit from fasting, herbal and other natural remedies, and profound approaches to prayer, meditation, and silence. Readers learn what characterizes the best monastic communities and discover a sense of the retreat experience as spiritual adventure. Featuring over 100 photographs, daily exercises, personal anecdotes, and fascinating snippets on monastic experience through the ages, "Wisdom from the Monastery" introduces readers to a cast of remarkable monks and nuns who have chosen lives of renunciation and simplicity. Within these pages, monastic wisdom demonstrates its relevance across millennia and beyond monastery walls.
About the Author
Peter Seewald s books with Cardinal Joseph Ratzinger (now Pope Benedict XVI), Salt of the Earth and God and the World, have been translated into 25 languages. Journalists Lucia Glahn and Simone Kosog live in Munich, and Bernhard Muller, a publisher, lives in Kesslegg, Germany."
Praise For…
"Featuring numerous daily exercises, advice and anecdotes, this compilation of words from monks and nuns who have chosen lives of renunciation and simplicity brings a calm perspective to such diverse endeavors as fasting for purification of the soul, moderation and orderliness. Even a brief read is enough to inspire a break for reflection."
—Whole Life Times


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AIKEN — Thirty-six holes wasn't enough golf to determine the City of Aiken Amateur champion.
Neither was an aggregate four-hole playoff. Brian Quackenbush and Bo McCullough needed eight holes of sudden death.
But with the sun dropping on the horizon, Quackenbush knocked in a four-foot birdie putt on the 12th extra hole – his 30th of the day – to win the title at Aiken Golf Club.
"I finally hit the green," Quackenbush quipped after his birdie on the par-3 18th hole, his fifth time playing the hole Sunday.
Quackenbush and McCullough each finished regulation at 2-under-par 138. A four-hole playoff was the format to determine the overall champion.
The golfers played the four holes in even-par fashion. Then in sudden death, they matched pars and birdies for seven holes.
Finally, Quackenbush's 5-iron shot on the 190-yard par-3 hole started at the flag and never left it. It settled four feet away.
McCullough's tee shot came up about 30 feet short, and he figured he must make it to continue the playoff. His effort just missed and rolled by five feet. When he missed that, Quackenbush tapped in his short birdie putt for the victory.
"I felt like I had to give that one a run," McCullough said.
Quackenbush's victory ended a two-year run as champion by left-handed golfer Patrick Cunning. The former University of South Carolina golfer mounted a solid defense with rounds of 70 and 73.
Quackenbush, 41, is a former professional golfer whose career ended because of an injury. He said he's been in long playoffs before, but none that went 12 holes.
He was familiar with his opponent; both regulars at Aiken Golf Club.
"I knew it was going to be tough," Quackenbush said. "He's so steady and a great putter."
Other winners Sunday were Jimmy Hamilton, seniors; Dick Korzen, super seniors; Jake Carter, juniors; Julia Fountain, ladies; and Hannah Griffith, junior ladies.


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The film is being billed as the South Korean "Ocean's 11", and it's easy to see why. The film, a heist flick involving lots of characters and a big fat diamond, features an incredible ensemble cast, so the correlation seems to make sense in this case. Since learning about the flick last year, I've been anxious to get my hands on new material. I suppose this teaser poster will have to suffice until a proper trailer finds its way into my giddy little hands.
As per usual, here's what the film is all about:
In order to let things cool down from their latest heist, Popeye and his group of thieves go to Macao on a job. But the mastermind behind this job is none other than Popeye's old partner Macao Park, who escaped with 68kg of gold several years ago on their last job together.
Macao Park brought Chinese thieves as well but little did they know what Macao Park planned for each one of them. But his plan takes an unexpected turn when Popeye brings Pepsi, an old flame of Macao Park, to settle the old score. The thieves' target is a $20 million diamond known as 'Tear of the Sun', kept safely away in a casino, brought there to be sold by a notorious Chinese fence. While working together to steal this fabled diamond, they all have their own agenda to keep the diamond for themselves. But who will succeed and live to see another day?
And, yes, you read that correctly: One of the main characters is named Popeye. Crazy! "The Thieves" stars Kim Yun-seok, Jeon Ji-hyeon, Kim Hye-su , Simon Yam, Angelica Lee, Kim Hae-sook, Oh Dal Soo, and Lee Jeong Jae. Look for it to hit South Korean cinemas this summer. The poster, as you can tell, has been embedded below.
Via : Film Smash


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Space available for rental to our MSMEsPopular
BEDCO offers office space to entrepreneurs who would like to rent out offices for their business at affordable prices. If you are looking for office space, please come to BEDCO's offices located at Sebaboleng, near Lakeside Hotel and meet our Estates department, which will give you details about how you go about renting an office space at BEDCO.
For more information call:
Mr Karabelo Maluke +266 58558266
Reception +266 22312094,
Email us at email@example.com
NOTE: If there is no vacant unit currently your application will kept for 24 months and you will be contacted as soon as a unit is available.
Ad ID : 2
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Sebaboleng, Lesotho,
+266 58 558 266
email@example.com (2)


text: Quote: Originally Posted by John Willett The Neumann KM 183 will, almost certainly not work at the frequencies you want. How do you know that from a frequency response graph that only goes to 20kHz? Just because it hasn´t been measured beyond 20k doesn´t mean it´s not picking up anything beyond 20k.


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4 pictures
I wanted to thank you for the wonderful job you did with Dixie (Tigerlilly). She is such a sweet little cat. She adjusted almost instantly to our apartment. She got a catnip toy today and has been playing with it all day, and thankfully has been scratching her scratching post instead of the furniture! We have decided to rename her Tigerlilly, hope thats ok with you, we are big Disney fans. She is such a joy to come home to everyday. She purrs like an engine all the time. Her favorite place to sleep is the box spring! She has a vet appointment next week, will let you know how it goes. I will also be sending you some pictures when I take more of her. Again thanks for saving her life, if you didn't we wouldn't have this awesome new member of our family.
Emily and Alex