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BRUCE NAUMAN / mindfuck
Bruce Nauman has reached an almost mythic status as a contemporary visual artist. He is one of the few living artists whose is revered as a Great. Hauser & Wirth present a new show of work by the Great artist: Bruce Nauman / mindfuck brings together a selection of works from Nauman's career whose central concern is the convergence of mind, body, language, sex, death and language. You know, only all the important things of life.
The first question that pops into this writers head is, 'What gives Nauman the right to make work like this?' Well, he's an artist. His job isn't to explain, it's to present a view of the world that is developed uniquely from his own (admittedly very wide) knowledge and experience. It's a point of view that has been developed from the ovum of his studio, the sancturary of that womb and the recognition of his own intellectual nascency. The display is predominantly comprised of installation and Nauman's trademark neon sculptures. The accompanying text and catalogue make much of the language of metapsychology that Nauman explores. It's a new reading of Nauman's work, and at points feels stretched, but for those of you with an interest in post-Freudian self-analysis, it's interesting stuff.
Because a show by Nauman is a show worth visiting. Take the themes of the work and take its interpretations. These are not things to be sniffed at. Nauman is taking the very fundamentals of what makes us human and both reduces and highlights its absurdity, contradiction, difficulty and -- ultimately -- its sheer power. Influential is not the right word to describe Nauman; it feels inadequate. Whether your interest in art is spiritual, corporeal, political or visceral, Nauman's work has something for you, and its something to mull over and keep with you. It's Great art.
Bruce Nauman / mindfuck is at Hauser & Wirth until 09 March.
header image:
'sex and death/double '69'', 1985
neon tubing on aluminium monolith
227 x 134.8 x 34 cm / 89 3/8 x 53 1/8 x 13 3/8 in
image © 2012 bruce nauman / artists rights society (ARS), new york / DACS london
private collection. courtesy hauser & wirth
photo: stefan altenburger photography zürich
second image
'run from fear, fun from rear', 1972 by bruce nauman
neon tubing with clear glass tubing suspension frame
two parts:
20.3 x 116.8 x 5.7 cm / 8 x 46 x 2 1/4 in
18.4 x 113 x 5.7 cm / 7 1/4 x 44 1/2 x 2 1/4 in
image © 2012 bruce nauman / artists rights society (ARS), new york / DACS london
private collection


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Four years after the North Texas Tollway Authority paid billions in the contentious State Highway 121 toll-road deal, the one-time cash infusion is being felt, seemingly, everywhere in Collin County.
Road projects totaling some $400 million are under way along U.S. Highway 75 in McKinney north into Melissa. Work zones dot U.S. Highway 380 from eastern McKinney into Frisco. Intersections and hike and bike trails are slated for improvement in Plano and Richardson.
Major roadwork also is planned or under way in Dallas and Denton counties. And $250 million in toll-road money helped pay for the A-Train project between Denton and Carrollton.
On the political front, the long-term wisdom of the NTTA's toll-road agreement continues to provoke debate and, in some quarters, even resentment. The agency gained control of the road project in exchange for an instant cash payment of some $3.2 billion. Toll-road drivers will face decades of escalating fees.
"When the money runs out, what then?" asked Frisco Mayor Maher Maso, a critic of the deal.
Yet in the short term, at least, the windfall is fueling one of the largest and most widespread road expansions in Collin County's history.
"I have to think, without a doubt, that this is one of the busiest times," said Robert Daake, executive director of McKinney's development division.
A large portion of the work is pegged for U.S. 75 in McKinney, a community where highways originally designed for rural traffic have become strained by suburban growth. Collin County's population has more than tripled since 1990 to about 800,000.
The Texas Department of Transportation has launched six expansion projects in a roughly 12-mile stretch of U.S. 75 from 121 to north of Melissa.
Endeavors include rebuilt interchanges at U.S. 380 and Melissa Road, a widened highway and frontage roads and other upgrades. Collectively, the projects will cost nearly $400 million and finish in various stages through 2015.
Money sprung from the toll-road deal also is paying for the completion of 121.
A new interchange has been completed on 75 at 121 in McKinney. And workers are well on their way to finishing a gargantuan ramp system at 121 and the Dallas North Tollway whose height rivals that of nearby office towers.
Almost $200 million in widening projects and other upgrades also have launched along U.S. 380, which has emerged as a bustling outer beltway serving some of Dallas' farthest-flung northern suburbs.
Slow traffic ahead
For the next few years, many suburban commuters are all but guaranteed to witness construction-related traffic tie-ups.
Toni Young, head of school at the North Texas Christian Academy, which faces southbound 75 in McKinney, said the roadwork has added about 10 minutes to her commute from Allen. That, she says, is a small price to pay for better roads.
But the real test will come when classes start Aug. 22 and parents have to navigate the traffic cones. With its multiple work zones, 75 and its frontage roads routinely back up during morning and evening rush hours.
"I grew up in the area, and I've never known any part of it without construction," Young said. "For me, it's all just a part of the norm. But other people that move in sometimes are just shocked at all the [road work]."
'Get it over with'
Like Young, Plano Mayor Phil Dyer takes a long view of all the road work. In Dyer's city, the toll road-funded projects include a $20 million overhaul of the interchange at 75 and Parker Road that opened in December.
"It causes some inconvenience, but let's go through it and get it over with," Dyer said.
Still, as Maso points out, the money from the toll-road deal will not last forever. While the bulldozers are out in force now, regional policymakers will soon face another round of funding choices.
"It's good to see these roads under construction," Maso said. "Frankly, the projects under way are badly needed. But we have to figure out what's next when the money runs out."
These are being funded by the State Highway 121 toll-road deal:
1. A series of widening and expansion projects totaling almost $400 million on U.S. Highway 75. Completion: In stages through 2015.
2. Interchange construction at Dallas North Tollway and State Highway 121. Completion: Sometime in 2012.
3. and 4. Sections of U.S. Highway 380 in Frisco and McKinney are getting upgrades. Completion: In stages through 2014.
5. Interchange overhaul at 75 and Parker Road. Completion: Finished late 2010.
SOURCE: Texas Department of Transportation; city of McKinney; North Central Texas Council of Governments

subset: commoncrawl


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Explosion in China Mine Kills 20
More than 30 workers remain trapped underground in Henan province.
An explosion in a Chinese coal mine killed 20 and trapped more than 30 workers underground on Saturday in the country's central region, Chinese state media reported.
The blast comes shortly after the world was riveted by Chile's dramatic rescue of 33 trapped miners after they spent more than two months underground.
China Central Television said the blast happened Saturday morning in Henan province. An official surnamed Wu with the province's coal mine safety bureau confirmed the accident but could provide no further details.
The state-run Xinhua News Agency cited mine officials in Yuzhou city as saying Saturday's 6 a.m. blast happened in a pit owned by Pingyu Coal & Electric Co. Ltd.
A man answering phones at the mine said he had not heard anything about an accident.
China's mining industry is the most dangerous in the world, and more than 2,600 people died in mining accidents last year.
The state-backed People's Daily newspaper reported Thursday that China has shut down more than 1,600 small, illegal coal mines this year as part of an effort to improve safety standards.
China mining fatalities have decreased in recent years as the government closed many illegal mines, but the death toll jumped again in the first half of this year.
In October, the Chinese State Administration of Work Safety said mine managers and bosses who do not accompany workers down into mine shafts would be severely punished.
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- WildEarth Guardians take aim at CPW predator managementtheheraldtimes.com
- Colowyo Mine gets go-ahead for expansionThe Daily Sentinel
- Utilities: Local input key when power plants closeThe Daily SentinelFront Range , Colorado , Montrose , West Slope , Palisade
- Trapper coal mine near Craig will stay openThe Daily Sentinel
- Review ruling allows Trapper coal mine near Craig to keep operatingThe Daily Sentinel
- Carbon conflictThe Daily Sentinel
- Trapper coal mine's environmental assessment releasedsteamboattoday.com
- Trapper coal mine's environmental assessment releasedcraigdailypress.com
- Moffat County coal meeting slated for Wednesdaycraigdailypress.com
- Comment period approaching for two Northwest Colorado coal minescraigdailypress.com
- The campaign against coalhcn.org
- Bowie Mine legal target of WildEarth GuardiansThe Daily Sentinel
- Trapper coal mine has until April to complete environmental assessmentsteamboattoday.com
- Tamale Town ends short time in Craig business scenecraigdailypress.com
- Colowyo review completed ahead of deadlineThe Daily Sentinel
- SOSMRE releases Colowyo environmental assessment ahead of deadlinesteamboatpilot.com
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Use the tools below to refine your search by only displaying reviews with a certain number of star ratings or to only show reviews from a certain time period. Eg click on '1 star' to just display the reviews we have which received a 1 star rating click or 'Within the last month' to display just reviews posted over the last month.
Written on: 23/02/2013 by johnHug (1 review written)
I am a rep and use the Astra doe business, I do about 45,000 miles per year, I bought the car with 63,000 on the clock for 1800 pounds. I have had a new timing belt,water pump and plugs fitted at 80,000 and thats it cost 150 ponds to have it done with a local mechanic. MPG is incredible for a petrol engine around 10 pence a mile aound town and long journeys I can get over 450 out of a tank full. Handling is good for its class I have the bigger wheels on mine ,tyres do about 20,000 on the front… Read Full Review
Written on: 10/04/2006 by 163567433378 (4 reviews written)
The Vauxhall Astra, a really good family car, good access, nice seats easy to find driving position. Visiblity all round good if you use the mirrors, quiet at motorway speed, alittle harsh in sport mode but quite lively. Around town lovely torquey engine makes for relaxed traveling. On the road 46 mpg is attainable, around town expect 34/36 mpg. Buy with confidence, and enjoy… Read Full Review
Written on: 17/09/2002 by Smiley. (1 review written)
The Vauxhall Astra is a good overall car - comfortable and stylish upholstery, with all the extras including air con, radio functions on steering wheel, lcd display for temp. Good on pickup with a lot of poke… Read Full Review
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text: Hey guys I had a question about California insurance I want to get an ballpark idea of what I'm gonna be paying. The main thing I'm worried about is auto insurance but I want to get an idea of what you guys in California are paying for auto, liability, and workman's comp. I called ins companies and they give me the runaround since I have not started up yet. Thanks for your input


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Anyone use K12 independent study curriculum?
Anyone use k12 independent study curriculum? Anyone know how it compares to their International Academy curriculum? Looking to make a switch next yr to save some money.
Quoting bugayfamilyof4:
Me either. Here is BUMP !!
I have used the K12 curriculum as an independent homeschooler for 10 years. I have a 7th and 9th grader. This year my son, 9th grader, was enrolled full time in K12's International Academy (iCademy). For K-8 the curriculum is the same whether you get it independently or through the iCademy. The level of support is different. As an independent homeschooler, you are not tied to a schedule or a teacher. iCademy has teachers, counselors, advisors, long term planning, aptitude testing, etc. that goes with a fully accredited school, just done at home. Both options offer clubs and a great support community.
I am a new to homeschooling. I will be home schooling my thirteen year old son. Our state uses the K12 program for their "Cyber" Academy. Unfortunately, for right now, he doesn't qualify for the Cyber Academy program (which is an online, tuition free school program) because he was expelled from school for the rest of this year and our school system doesn't offer a good alternative program. This is not cheap by no means, but I want to be able to have lesson plans already done for me versus just buying the subjects and making my own.
Has anyone else started to use this program since the last post last year?


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Search Results
Entries tagged with "
Real World"
December 14, 2010 8:48 PM
The Real World tagged "Real World"
If there's one acronym I hate, it's this one: IRL. IRL stands for: In Real Life. People talk a ton about what they're doing online versus what they're doing in the real world. People often talk about meeting-up in the... Read more
March 27, 2010 8:20 PM
The Truth About Online Reputation Management tagged "Real World"
Here's another dirty little secret about your brand and how you manage your online reputation... There's no difference between what's being said online and in the "real world." In fact, let's call a moratorium on the separation of the two...... Read more
July 12, 2009 9:22 AM
Augmented Reality - A Marketer's Primer tagged "Real World"
If you're looking to stay slightly ahead of the Digital Marketing curve and wondering if virtual worlds like Second Life are going to make a comeback, Augmented Reality could well be "what's next" and a huge skip past virtual worlds.... Read more


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Barking Mad To Run
On Saturday, January 12, 2013, I participated in the Resolve for A Better San Antonio 5K, put on by the Junior League of San Antonio. Below is the link to the photos and report that I did for this event. Please feel free to share the photos and report.
For those that got 'unknown error" sorry about that! I think I forgot to do the little 'linky connect' thing above. I did that now and I just tried the link again after doing that and it seems to work now.
"Do what you can, with what you have, where you are." Theodore Roosevelt
An unknown error has occurred. The site administrator has been notified.
I get this when I click the link
You may be right
Me too.
Unknown error
An unknown error has occurred. The site administrator has been notified.
I get this when I click the link
Don't believe everything you think.
Smaller By The Day
Do you know what I love about your race reports? Not only are you smiling in every picture, but everybody you take a photo of or with is smiling as well. You must be just as awesome in person as you are online.
Weight 100 pounds lost
5K 31:02 Sept. 2012 / 23:36 Sept. 2013 (Same Course)
10K 48:59 April 2013
HM 2:03:56 Nov. 2012 / 1:46:50 March 2013
MARATHON 3:57:33 Nov. 2013
I was able to see it
Another good race Scotty!
Link worked for me. Another great Scottydog RR!
"It's not the mountains we conquer, but ourselves."
~ Sir Edmund Hillary
Congrats on another great race and RR, Scotty!
Upcoming races:
Covenant Health Knoxville 5K 4/7/13 (and cheering on DH for his 1st Marathon!!)
Rock and Roll Chicago Half Marathon 7/21/13
Tower of Terror Ten Miler 10/5/13
Miles to Go
Run Scotty Run !!!
Last:: 8/27 Fort2 Base 10NM, Great Lakes, IL
Next: 10/22 ScotiaBank Waterfront HM
That is great scenery for a race- what a nice course! I see what you mean about the size- too many participants and somebody is going to end up in the river!
It sort of felt like you had "home court advantage" for this one!
That almost makes up for the "clock tower" race last year, right?
Don't call me Buttercup!
You always find races that are pretty and/or interesting to run. I'm jealous! =)
Slow and steady wins the.... wait a second! I've been lied to!
Congratulations on another race, Scotty. What a lovely, historical course!
"We do not become the people who this world needs simply by turning our backs on anyone we don't like, trust, or deem healthy enough to be in our presence. " ---- Shasta Nelson
The link worked for me this time. Great job on the race. I've only read two of your RRs (because I haven't been in this forum very long) and I would like to say they were very interesting. I enjoy the pictures, makes me feel like I was there with you. I look forward to reading many more!
How's the Alamo beer? We don't get it here.
Congrats, Scott, on your 5th race of the year! 5 already, wow! I love when you take pictures of San Antonio, such a beautiful town... Life must be good, there. And great people as well! So now we know that not only do you have a weakness for great running legs, but you also have one for lovely volunteer ladies with beautiful smiles. Old dog you.
PRs: Boston Marathon, 3:27, April 15th 2013
Cornwall Half-Marathon, 1:35, April 27th 2013
18 marathons, 18 BQs since 2010
It is QUITE good. They had a choice of a darker lager and a "light blonde" lager. I chose the light blonde, so I wouldn't have to have a lengthy conversation with it....


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> Career Resources
> Interviews > Industry Updates > Sales / Marketing
English proficiency offers a world of opportunities
In order for Hong Kong to maintain its position as a world city and remain competitive as a regional financial centre, it is essential that people are able to communicate effectively in the English language, particularly in commercial settings.
"Learning a foreign language means learning to communicate with people from other parts of the world. Simply being proficient in the language is therefore not enough. People must learn how to use it in the most effective way," says Sam Cheung, president, Englishtown.
With nearly 15 million users from more than 75 countries, Englishtown is backed by EF Education, the world's largest brick-and-mortar language institute. It is the largest specialist English-language training organisation in the world, with offices in Europe, Asia, North and South America.
The official language training services supplier for the recent Beijing 2008 Olympic Games, Englishtown's method of instruction combines face-to-face lessons with social activities and an interactive iLab, giving students the chance learn and apply the language in a completely English environment.
The organisation has put US$50 million into developing and improving customised course materials and has also partnered with world-class facilities such as the highly regarded Harvard Business School Publishing to ensure outstanding product development.
Development opportunities
With more than 40 years of experience, the institution has more than 450 offices and schools across the globe and employs 27,000 professional teachers worldwide.
Locally, Englishtown has a dedicated team of course consultants to perform the school's sales functions, maintain good customer relations and participate in marketing activities and social events to help drive sales and customer referrals.
"To be a successful course consultant, candidates should be good team players who are proactive and sales-and performance-driven. They must also possess good communication skills and be able to work independently," Mr Cheung points out.
He adds that it is also important for recruits to adhere to Englishtown's core values. The organisation's employees work hard and play hard. This means that they are passionate about everything they do, live life to the full both at work and at home, and always strive for balance.
The organisation places a high premium on a positive attitude and entrepreneurial spirit, which are regarded as forces for good, and staff members are expected to be constructive in times of challenge and change, Mr Cheung notes.
"Detail is important. If you drop the ball, a participant's experience can be ruined. Stay on top of the little things, but never lose sight of the big picture," he advises, adding that the ultimate focus is on results.
The minimum requirement for sales professionals interested in joining the team is a diploma and three to five years of direct or consultative selling experience. Excellent communication and telephone skills plus computer and internet literacy are essential.
Sound financial judgment is an attribute. "Our team members must always think about effective cost management. This is not the same as cutting costs, because sometimes cost-consciousness actually means spending more on the right ideas," Mr Cheung stresses.
Structured careers
Englishtown offers new recruits excellent training opportunities. This includes participation in the EF orientation programme, which equips them with the organisation's core values right from the start. Meanwhile, department heads provide product and sales training to ensure that new employees quickly absorb essential business know-how.
Recruits embark on a structured career path with room to move not only vertically but also across products. A typical promotion route can take a course consultant from senior course consultant, assistant centre manager to centre manager and eventually to the position of sales director.
The right candidates are assured of long-term career development opportunities on a regional and global level. Employees who excel may find themselves working in overseas MBA or university programmes, or in financial or insurance products.
"We offer a strong and unique culture within a truly multinational company, where people can grow," Mr Cheung notes, adding that the organisation has a flat hierarchy with no office politics. "This essentially makes a difference to the working environment and the way employees feel about being part of the team," he concludes.
Free Subscription


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You can try these similar gifts instead.
Who We Are: Questions to Celebrate the Family Book
Every family is different, but they think that their family is different in a way that makes them the best. This book is a fun conversation starter for every generation that lives beneath their roof.


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An immigrant from County Mayo, Charlie O'Kane is the American Dream come true, as he rises from immigrant Brooklyn beat cop to District Attorney, Brigadier General, Mayor of New York, and then ambassador to Mexico.
In 1941, when Charlie was district attorney, Abe "Kid Twist" Reles was Charlie's star witness against the biggest crime syndicate in New York. While under 24-hour police watch, Reles jumped - or was he pushed? - out the window of the seedy Coney Island motel where he was being hidden, and with him went Charlie's case.
Over a decade later, Charlie's younger brother, Tom, finds himself investigating Charlie, who has been in exile in Mexico with his beautiful young second wife. His reputation in shreds, with accusations of mob connections and corruption stemming from Reles' death, Charlie's only hope of being cleared rests with the adoring Tom, who still believes Charlie is innocent, despite the ever-mounting secrets between them and his growing suspicions about his baffling brother.
©2013 Kevin Baker (P)2013 Recorded Books
"Evocative but a little disappointing"
This is a story of political corruption, intrigue, murder, Irish immigrants, adultery and New York in the 40's and 50's. That's a little too ambitious. Some of the street scenes and crowd scenes are overwritten and transparent attempts to be evocative. Surprisingly, the scenes in Mexico are very evocative of a lost time. Many of the characters, especially the gangsters are vividly described. But at times the book seem to veer into being a romance novel. It is not a bad book, just a little sprawling. The solution to the central mystery was clever but took so long, it was not that surprising.
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A Furthur After-Party/Art Party hosted by Gnomes Grown
with music by Stimulus Clan & Eelko / Gratefully Deadicated (DJ) and The Carnival Parade (DJ)
Thursday, Apr 12, 2012 11:30 PM EDT
Sullivan Hall, New York, NY
18 years and over
Purchase Tickets
Additional Information
Stimulus clan is the solo-side project of Jonny G, guitarist in death-funk glitchtronic trio, "lespecial". With field recorded samples, extensive synthesis exploration, and a keen focus on bangin' drum beats, he explores idioms all over the electronic spectrum. Expect to hear his own take on trip hop, drum n bass, and glitch hop. He cites Flying Lotus and Eskmo as huge influences.
Eelko, born Christopher Carchedi, is a live performing artist hailing from Boston who, with his music, blurs the lines between hip-hop, funk, trance, dubstep, and a plethora of many other influences.
Drawing inspiration from a love of avante garde jazz and free form 'jam' music, Eelko's live performances are always fresh and interesting.
Gratefully Deadicated DJ's Grateful Dead music and mix it in a seamless set. Playing the legendary Grateful Dead songs and jams from memorable concerts woven together to create the ultimate and unique Grateful Dead experience. Tracks are chosen from my high quality collection of cds and dats consisting of over 1500 Grateful Dead concerts.
The Carnival Parade began on December 30th of 2005 @ The Knitting Factory as a Mule/Bisco Afterparty. It featured Steve Urban on Laptop/Guitar/Vox spinning remixes of The Grateful Dead along with other popular acts in the jamscene. Rumor has it that Warren Haynes was even in attendance. Jambands.com called it: "A rather unique one-man show which mixes live guitar, vocals, DJ effects, and computer mash-ups." The Philadelphia Weekly wrote, "While the music ranges from rock to jamband to reggae and funk a commitment to a positive socially conscious message forms a common bond. Good people, great music and positive vibes."
In the Fall of 2008 Urban is introduced to guitarist Rob Varkony of Star'69 at a Phil Lesh & Friends Afterparty and begins sitting in with them at weekly jam sessions in the East Village. In 2010 & 2011 Urban performed at a A NIGHT FOR DAVE NOLAN a Benefit Concert at Brooklyn Bowl along side Chris Barron from The Spin Doctors & The Zen Tricksters. The concert was in the memory of Dave Nolan who was the radio host for WBAI's "Morning Dew" and the DJ of The Wetlands Preserve, a legendary NYC rock club. Dave was instrumental in helping the careers of many artists such as Dave Matthews Band, Spin Doctors, Blues Traveler, moe. and Warren Haynes. He was the first person to ever play many of these artists on the radio. Steve Urban became the last in a long line of artists which Dave brought to recognition among the NYC music scene. In 2010, Urban then met accomplished bassist Greg Koerner of Gent Treadly, a friend of Dave's as well. That's the story of how The Carnival Parade (Live Band) was formed... The rest is rock & roll history!
*Any person entering the venue that is under 21 years old will have to pay an additional $5 " under age fee " on top of the ticket price. The under aged fan that is paying this fee will get 1 free soft drink ticket so they can get 1 free soft drink at the bar.


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You would have to flash a custom 2.1 rom to have apps2sd as well as a 2nd partition on your sd card
Sent from my HTC Hero using Tapatalk
1. Backup your SD Card
2. Download Amon Recovery
3. Format your SD Card (32Mb SWAP, 512Mb EXT2, FAT32 rest)
4. Convert EXT2 to EXT3
5. Flash new ROM with APPS2SD support
XDA Developers was founded by developers, for developers. It is now a valuable resource for people who want to make the most of their mobile devices, from customizing the look and feel to adding new functionality.
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Joined: 02 May 2006
Posts: 9572
The following 2 member(s) liked the above post:
screen22, Captain_Pari,
Proof the girl is blazing like fire
Time indeed flies by.
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text:
A controversial plan by U2 guitarist The Edge to build a cluster of mansions on 156 acres overlooking Malibu could get new life from a bill being promoted in Sacramento.
The California Coastal Commission nixed the development last year, citing concerns that it would irrevocably damage the environment.
But now some of the same lobbyists and lawyers behind the rejected proposal are pushing legislation that goes to the heart of how government agencies determine property ownership, and how they use the findings in deciding whether to approve development, the Los Angeles Times reported Saturday ( http://lat.ms/Ls5oE7 ).
The property owners maintained they are five separate owners each building a single home on separate lots atop a scenic bluff, an argument that would have made it more difficult for the commission to reject their proposal.
The commission, however, determined last June that there was enough evidence to show the project was under common ownership, coordinated and led by The Edge, whose real name is David Evans.
Current rules say the Coastal Commission has authority to approve projects in environmentally sensitive areas, and it takes ownership into account. For example, the agency is more inclined to reject a plan for a multi-home project than a proposal to build a single-family house, according to the Times.
Under the bill, authored by Democratic Assemblyman Ben Hueso, state agencies would have to accept as fact that the person holding the deed is the property owner, the Times said. If the state sought to challenge true ownership, it would be held to the same evidentiary standards that apply in the court system.
The Coastal Commission and others argue that the standards would hinder public agencies because they have none of the court system's tools of discovery: subpoenas, depositions and sworn testimony.
Sarah Christie, legislative director for the Coastal Commission, told the newspaper the law would curb public agencies' abilities to fulfill their missions, giving any developers who game the system more clout and potentially leading to "more fragmented, inappropriate development" along the coast.
Environmentalists slammed the bill too.
"The forces who want to maximize their profit above all else are seeking to rewrite the rules in such a way that ensures the state government has limited ability to oversee and to take care of our public trust resources," said Adam Keats, an attorney with the Center for Biological Diversity, told the Times.
Hueso said he introduced the bill to rein in what he sees as an overzealous bureaucracy that uses arbitrary standards to block development, a notion shared by the California Chamber of Commerce and other business groups.
"If we have every agency in California cherry picking projects they don't like, it's going to create an enormous problem in our ability to do business in California," he told the Times.
The bill's purpose is not to advance the interests of the U2 guitarist, Hueso said. The lawmaker told the newspaper he got the idea for the legislation from Paul Bauer, one of the lobbyists The Edge hired last year to help him before the Coastal Commission.
Fiona Hutton, a spokesperson for The Edge, told The Associated Press the guitarist isn't paying anyone to lobby the bill. However The Edge and the other property owners "are actively working now to achieve a solution through the Coastal Commission process," Hutton said Saturday.
The bill's text echoes language at the heart of lawsuits The Edge and his partners have filed against the commission, according to the Times. Evans' attorney, Stanley Lamport, is also lobbying lawmakers to pass the new legislation.
Lamport told the newspaper his involvement had nothing to do with The Edge and that he was acting in his capacity as the legislative chairman of the California Business Properties Assn., the sponsor of the bill.
Information from: Los Angeles Times, http://www.latimes.com


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|News Home | Video News | Early Brief | Forum | Passdown | Discussions | Benefit Updates | Defense Tech
Tillman Cover-Up Investigated
Associated Press | March 07, 2006WASHINGTON - The Defense Department will investigate allegations of an Army coverup in the shooting death in Afghanistan of Cpl. Pat Tillman, in addition to a criminal investigation into the 2004 killing of the former NFL star, the department said Monday.
Gary Comerford, spokesman for the Defense Department's inspector general, said his office's review of three previous Army investigations of Tillman's death - none of which was a criminal probe - "found things that should have been looked at."
The spokesman would not elaborate. Other officials said the inspector general concluded that the earlier Army investigations had produced enough evidence to merit probing possible charges of negligent homicide. The officials would discuss the matter only on condition of anonymity because the probe has not yet begun.
The death investigation will be conducted by the Army's Criminal Investigation Command. Comerford said the Defense Department inspector general is "looking into other issues raised by the Tillman family and by some members of Congress," adding, "It's not like this is over. This is still an ongoing issue here."
Comerford said it would be contrary to inspector general policy to provide more details. But other officials said the "other issues" include allegations of a cover-up, which the Tillman family has made.
In addition, the officials said the department could examine the Army's failure to notify Tillman's parents before his nationally televised memorial service in May 2004 that he was not killed by enemy fire, as the Army had originally told them. Army investigations concluded that he was mistaken for the enemy and gunned down by his own men.
The Army has publicly acknowledged that it erred by not telling the family earlier that Tillman was killed by fellow Soldiers.
Gen. Peter Pace, the chairman of the Joint Chiefs of Staff, said Sunday that while there is no evidence as yet of a crime in the shooting death, Army investigators will want to find out if any of Tillman's fellow Soldiers were "firing a weapon when they should not have been."
Three other U.S. Soldiers were wounded in the gunfight, which occurred April 22, 2004, near the Pakistan border. It was not clear Monday whether the circumstances of those woundings would be included in the Army criminal investigation.
Tillman's parents have been outspoken critics of the Army's handling of the shooting death. They have accused Army officials of lying, concealing relevant facts and failing to adequately punish the Soldiers involved in the shooting. Because of the family's objections, the Army last August asked the Defense Department IG to review its initial investigations.
Some of Tillman's fellow Soldiers were given administrative punishment, including one for dereliction of duty for failure to supervise and control the gunfire that was directed at Tillman during what the Soldiers thought was a gunfight with enemy forces.
Tillman, who played defensive back for the Arizona Cardinals of the National Football League, joined the Army Rangers after the Sept. 11, 2001, terrorist attacks. His enlistment drew wide attention because of the lucrative NFL contract he gave up to join the Army.
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Emails showing blank on Windows 8
I'm having an issue I can't seem to fix or find a solution online.
When sending emails to myself in PHP, using codeigniter's built in email class, my emails are coming up blank in the Windows 8 mail app, however it shows normal on my web based email. I've tried different email accounts but it all does the same thing. I thought part of it was the email being too long, seeing as I used to load most of the site's css into the head of the html, but even when taking that out and using inline styles, it still shows up blank, any ideas? The only external source I'm using is the site logo in the top of the email, but I don't see why that would pose an issue for the mail app, especially considering I remember seeing messages to "click here to reveal images" in the app.
Also, in regards to the logo, is it possible to send it as an attachment instead rather than an external link? I've seen this done before as well. If not, what are the other alternatives besides just typing the website name out? I want to include the logo if possible.


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PSNI detectives investigating the murder of Garda Detective Adrian Donohue have made a specific appeal for information.
Det Garda Donohoe was shot dead two weeks ago during an armed robbery at a credit union in Co Louth.
Police believe the gang involved travelled in a dark coloured Volkswagen Passat from Dundalk to Fews Forest outside Newtownhamilton, Co Armagh.
The car was abandoned and set on fire.
They want to speak to anyone who saw a dark coloured Volkswagen Passat in the area on the night of 25 January after 9.30pm or who may have seen any other vehicles in the area around this time.
They also want to speak to anyone who observed fire, smoke or burning vehicles in the area.


text:
NASA image: Fires in Argentina Sept. 11, 2013September 11, 2013 in Earth / Earth Sciences
Wildfires have broken out in four provinces in Argentina including forest land in Cordoba. The high temperatures and gusty winds have wreaked havoc on the growth of these wildfires and the local meteorologists predict more of the same conditions in the coming days.
According to the Miami Herald: "Cordoba Social Development Minister Daniel Passerini said Tuesday that firefighters are having the toughest time in the central province (near Cordoba) with flames fanned by wind gusts and high temperatures. Passerini says the blaze in Cordoba province has caused the evacuation of 480 people, most of them in the tourist zone of Calamuchita Valley. He says one man is in grave condition after being struck on the head by a tree."
This natural-color satellite image was collected by the Moderate Resolution Imaging Spectroradiometer (MODIS) aboard the Aqua satellite on September 10, 2013. Actively burning areas, detected by MODIS's thermal bands, are outlined in red. NASA image courtesy Jeff Schmaltz, MODIS Rapid Response Team. Caption: NASA/Goddard, Lynn Jenner with information from the Miami Herald
Provided by NASA's Goddard Space Flight Center
"NASA image: Fires in Argentina Sept. 11, 2013" September 11, 2013 https://phys.org/news/2013-09-nasa-image-argentina-sept.html


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Patrol Deputy is open!
We are pleased to announce the opening of Patrol Deputy! Please read below to find out more about this great opportunity!
Patrol Deputy - This is a full time, commissioned position working the Field Services Division of the Ada County Sheriff's Office. General duties include: Patrolling designated areas, determining if a crime has been committed, enforcing laws, writing citations, investigating traffic accidents, making arrests, and transporting arrestees. Candidates for this position must be at least 21 years of age, be a US citizen, and possess a valid Idaho driver's license (by date of hire). Additionally, candidates must have 64 semester credits or an Associates Degree from an accredited college/university; or two years prior full time experience in law enforcement with a recognized public safety agency as a commissioned or licensed law enforcement officer; or have served four years consecutive and full time military experience. Hiring salary is $13.56-$14.42 per hour, depending on experience/education. Patrol Deputy will close on April 2, 2004.
To learn more about this position or other great opportunities at the Ada County Sheriff's Office, please visit our website at www.adasheriff.org. Thanks for your continued interest in employment with our agency!
Kerry Davis
Ada County Sheriff's Office
7200 Barrister Drive
Boise, ID 83704


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Hello Mohit, First go to the page of http://bhulekh.up.nic.in/ here you will have to click on the district option of Ghaziabad. There is two tehsils online land record khata khatooni is available for the tehseel Ghaziabad and second for the tehsil Modi Nagar. As per you details you have your property in Modinagar so click on the option of Modinagar to get the online khata khatoni of Uttar Pradesh Online land record. After that click on the village option of Kasampur and then click the next proceed button. Here You will get the information for following view.
District - Ghaziabad
Tehsil - Modinagar
Village - Kasampur
After that you will have to select the option to get the khata khatoni online bhulekh land record with the Khasara Number, Khata Number and Name.
Select one of them and fill your details. After that you can get the printout of your online land record khata khatoni bhulekh Uttar Pradesh UP State of Ghaziabad District.


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Running Hyper-V on Windows 7!
General discussion
All replies
No but it is possible to run JUST the Hyper-v server (the command line only server) and use the RSAT tools on your Windows 7 box to create and admin your vm's which are running on this box. So there are a couple of caveats here:
1. You need Hyper-V server 2008 (or 2008 R2) running on a true VT enabled server (IT'S FREE So download it.)
2. The RSAT tools on your Windows 7 box
You could also use Vmware Workstation to load the Hyper-v 2008 server as a proof of concept so you can see what it looks like.
It works pretty well in that fashion.
Hope this helps.
Windows 7 doesn't have feature to install Hyper-V, but if you have Server2008 or 2008 R2, on the top of that you can even install Windows 7 as virtual machine.
You need to changes BIOS to enabled Virtualization Technology. and then open Server Manager and add new roles select Hyper-V.
After reboot, click new on server manager, select virtual machine. will be good if you have .iso images of ghost OS.
I feel its not the comprehensive but definitely help you.
Yes. Is possible. I'll have to download and install this update Windows6.1-KB958830-x64-RefreshPkg.msu. When you finish to install, go to control pannel -> programs and features -> active and desactive system features. Then check Hyper-V tools. It is all.
If your processor doesn't support the virtualization requirements of Hyper-V then you won't be able to install it on that system. You could try Virtual PC instead, its not as robust as Hyper-V but for some people its all they need.
Kurt Dillard http://www.kurtdillard.com
If you really wanted to play around with Hyper-V from Windows 7, you will need to run or install VMware Player and give it whatever is available in your system, When done with the installation create the VMs from it. Remember this might slow down your computer if you are not running Intel i5 or i7 or AMD Opteron 12 or 18-Core. I suggesting getting AMD 18-Core CPU. Hope this helps you.
that KB is for RSAT . when you Install that you just mange administrator Tools that Run on other Server such As DNS Server , Hyper-v and so on . you can't Use this KB to install hyper-V lpcally
you can install Hyper-v on windows 8
Just a little more detail to this answer: On windows 7 the menu items you have to select are:
control panel
programs and features
Turn windows features on or off
Remote Server Administration Tools
Role Administration Tools
Hyper-V Tools
Easy huh! :)
Just a little more detail to this answer: On windows 7 the menu items you have to select are:
control panel
programs and features
Turn windows features on or off
Remote Server Administration Tools
Role Administration Tools
Hyper-V Tools
Easy huh! :)
Thanks a lot,
You have NOT stated the very major thing, which is that in Windows 7 Ultimate and may be likely in Windows 7 professional, the Hyper-V is available, however, in the Widows 7 Home premium isn't possible since the options above are missed.
PS. I was notified by TechSupportForum.com newslette as a Tech Support Forum Member to download Free Backup for Hyper-V & VMware , do you think it is good to go ahead as long as I am only using Oracle VM VirtualBox.
A man should convert his anger and sadness into strength to continue living in this life.
- Edited by cooperator Wednesday, September 30, 2015 11:23 PM


text:
Ask the Doctor About Lymphoma
Lymphoma Research Foundation's (LRF) Ask the Doctor About Lymphoma programs are designed specifically for people affected by lymphoma. These programs provide the latest information about lymphoma and its treatments to local communities across the United States. Ask the Doctor programs are two-hour topic-specific seminars that feature a presentation led by a leading lymphoma specialist followed by an extensive question and answer session. Ask the Doctor programs are available free-of-charge for members of the lymphoma community.
Topics vary, but often include:
Upcoming Programs
The information provided at any "Ask the Doctor" program is for general information only. The information and opinions provided by the medical doctors are general in nature and should not be used in place of appropriate, one-on-one, medical consultation. Should you have any questions about this program, please contact the LRF Helpline.


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The Scientific World Journal
Volume 2014 (2014), Article ID 863049, 9 pages
Interaction between DNA and Trimethyl-Ammonium Bromides with Different Alkyl Chain Lengths
Department of Physics, Wenzhou University, Wenzhou 325035, China
Received 31 August 2013; Accepted 29 October 2013; Published 15 January 2014
Academic Editors: I. W. Kuo and P. Orea
Copyright © 2014 Chao Cheng and Shi-Yong Ran. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
The interaction between λ—DNA and cationic surfactants with varying alkyl chain lengths was investigated. By dynamic light scattering method, the trimethyl-ammonium bromides-DNA complex formation was shown to be dependent on the length of the surfactant's alkyl chain. For surfactants with sufficient long alkyl chain (CTAB, TTAB, DTAB), the compacted particles exist with a size of ~60–110 nm at low surfactant concentrations. In contrast, high concentration of surfactants leads to aggregates with increased sizes. Atomic force microscope scanning also supports the above observation. Zeta potential measurements show that the potential of the particles decreases with the increase of surfactant concentration (CTAB, TTAB, DTAB), which contributes much to the coagulation of the particles. For OTAB, the surfactant with the shortest chain in this study, it cannot fully neutralize the charges of DNA molecules; consequently, the complex is looser than other surfactant-DNA structures.
1. Introduction
Cationic surfactants, a kind of agent to induce DNA condensation [1], have been used in DNA extraction [2, 3], purification [4], enhancing the fluorescence intensity [5], and have potential uses in gene delivery [6, 7]. Because of its technological and biomedical importance, extensive studies have been carried out to elucidate the mechanism of DNA-surfactant interaction [8, 9]. It is commonly believed that hydrophobic force contributes much to the binding of surfactant molecules to the DNA molecules. The induced DNA compaction can occur well below the surfactant's critical micelle concentration because the DNA chain can act as a backbone for surfactants to aggregate. The process was displayed to be a discrete all-or-none type transition at the single DNA molecule level and to be a continuous transition at the DNA ensemble level [10]. Many factors, including surfactant concentration, incubation time, salt, temperature, DNA conformation, surfactant's chain length, and surfactant headgroup, affect the interaction between surfactant and DNA [11–16].
With the advantage of imaging DNA molecules directly, atomic force microscopy (AFM) [13, 17], fluorescence microscopy (FM) [10, 18–21], and electronic microscopy (EM) [22, 23] have been used to study the conformation of DNA-surfactant complex. Single molecule methods, which can monitor the process of DNA-surfactant interaction, are also useful tools to reveal the mechanism and the complex's structure [13, 24].
Dynamic light scattering (DLS) method [25], another useful technique, can detect the changes of size distribution of DNA-surfactant complexes by measuring their Brownian motions. In this way, the coil-globule transition of DNA molecules can be monitored in bulk directly. Several studies have used DLS to study the interaction between DNA and cationic surfactants [26–30] or other agents [31–34]. For example, Dias et al. studied the compaction and aggregation of DNA induced by CTAB using DLS [27]. Using this method, the compacted DNA molecules could be monitored by the appearance of a band with low hydrodynamic radius and by the decrease in the intensity of the peak corresponding to extended DNA molecules. They also found that at intermediate surfactant concentrations, compacted conformation coexists with extended one, which agrees well with the fluorescence microscopy observation. Marchetti et al. found that the complexation process reaches a maximum when the number of surfactant (CTAB or DDAO) and DNA phosphate groups is equal. The compacted DNA molecules were also found to have a higher thermal stability than the elongated ones [28].
Despite the extensive literature to investigate the DNA-surfactant interaction, this issue still needs more investigation. For example, though previous studies have concluded that high concentrations of cationic surfactants lead to aggregation, less experimental research elucidated why the complexes tend to aggregate and finally have larger sizes. Moreover, how the length of the surfactant affects the DNA conformation needs to be clarified further.
In the present study, we aim to provide more clues on the previous problems by studying the interaction between a long-chain DNA (λ-DNA) and cationic surfactants with varying lengths [octyl-trimethyl-ammonium bromide (OTAB), dodecyl-trimethyl-ammonium bromide (DTAB), tetradecyl-trimethyl-ammonium bromide (TTAB), and cetyl-trimethyl-ammonium bromide (CTAB), Figure 1(a)]. DLS method was used to measure the size distribution. The zeta potentials of the condensed DNA particles were characterized under varying surfactant concentrations and chain lengths. As a complementary method, AFM was used to obtain the morphologies of the corresponding particles.
2. Material and Methods
2.1. Materials
OTAB, DTAB, TTAB, and CTAB (Figure 1(a)) were purchased from sigma and used without further purification. Bacterial λ-DNA (0.5 μg/μL, 48 500 bp) was purchased from New England Biolabs. 1x TE buffer (10 mM Tris-Cl + 1 mM EDTA, PH 8.0) was used to dissolve the surfactants. Distilled water obtained from a Milli-Q system was used in all sample preparations.
2.2. DLS and Zeta Potential Measurements
A Malvern Zetasizer Nano-ZS90 instrument was used for particle size and zeta potential measurements. The light source is a He-Ne laser with a wavelength of 633 nm. The laser power is automatically attenuated in order to make the count rate from the sample is within acceptable limits. Clear disposable capillary cells were used. Firstly, the cell was loaded with 0.8 mL diluted DNA solution (1 ng/μL in 1x TE buffer). The concentrated surfactant solutions (10 mM for CTAB, 50 mM for TTAB, and 100 mM for DTAB and OTAB) were injected to the above DNA solutions to reach the final surfactant concentrations for study (Figure 1(b)). Every sample was incubated overnight to make sure the system reached a thermodynamically stable state. The sample was placed in a thermostated bath maintained at 25°C. The scattered light was detected at an angle of 90°.
2.3. AFM Imaging
A SPM-9600 system (Shimadzu, Kyoto, Japan) was used for scanning. A probe with a spring constant of 42 N/m and a resonance frequency of 320 kHz was used. All samples were scanned in the tapping mode at an ambient condition. All presented images were flattened to improve the contrast grade. The imaging was performed at a scan size of 5 × 5 μm. All displayed images are height ones. For sample preparation, 10 μL incubated solution was dropped onto a freshly cleaved mica surface and incubated for 3 min at room temperature. The surface was then dried with a gentle nitrogen gas flow. After drying, the samples were kept in a desiccator ready for scan (Figure 1(c)).
3. Results
3.1. Size Distribution and Particle Size Measurements
Firstly, we performed DLS measurements of the DNA solution. The concentration of the DNA solution was 1 ng/μL. A representative intensity weighted size distribution of the DNA solution is shown in the top curve of Figure 2(a) with a peak showing a typical hydrodynamics radius of around 300 nm. This peak reflects the translational diffusion of DNA. However, it should be noted that the DLS measurement of the DNA solution in our study was not repeatable enough. Sometimes it exhibited more peaks with smaller radius, which may correspond to the internal dynamics of the DNA molecules. This should be attributed to the DNA molecule exhibiting a random coil conformation instead of a compacted spherical particle conformation.
The DNA samples with varying concentrations of surfactants added and incubated were then measured by DLS to obtain their size distribution curves. Typical intensity weighted distribution curves in the presence of different concentrations of surfactants are shown in Figure 2. For a CTAB concentration of 5 μM, two peaks with a hydrodynamics radius of 42.6 nm and the other 223.4 nm appeared; this observation suggests the compacted DNA molecules coexist with DNA coils, as revealed by previous studies. Increasing the CTAB concentration leads to the disappearance of the larger-radius peak. However, as the concentration of CTAB increased to 60 μM, a peak with a hydrodynamic radius of 384.1 mm is observed. Meanwhile, the peak with smaller radius is still detectable. With further increasing of the CTAB concentration, the magnitude of the peak with larger radius increases, while the intensity of the smaller-radius peak decreases. At 1 mM CTAB, only one peak with a radius of 203.2 nm is presented (Figure 2(a)).
The appearance of larger particles at CTAB concentrations above 60 μM indicates the aggregation of CTAB-DNA particles. The coexistence of small compacted particles and larger aggregates, similar to the concomitance of coil chains and compacted particles elucidated by FM study, has not revealed before as far as we know. Then, whether decreasing the length of the surfactant chain leads to similar results is of concern.
As shorter-chain surfactants, TTAB and DTAB, can also cause DNA compaction and larger particle aggregates. It can be seen that increasing concentration also leads to peaks with increased sizes. In our study, the surfactant concentrations at which a considerable increase of the particle size occurred are 1 mM for TTAB and 8 mM for DTAB. However, no curves reflect the coexistence of two peaks indicating the smaller and larger particles (Figures 2(b) and 2(c)).
Interestingly, the bimodal size distributions appear again for the surfactant with the shortest chain, OTAB. One peak has a smaller radius, while the other shows a larger size (Figure 2(d)). Nevertheless, both cannot be attributed to the presence of compacted particles similar to that observed in the CTAB-DNA system, which can be verified by the following AFM characterization.
Figure 3 shows the hydrodynamic radius of the particles obtained from the size distribution curves as a function of surfactant concentration. It can be concluded that the longer the surfactant's chain, the less surfactant molecules are needed to induce DNA condensation, so does the critical concentration at which a considerable increase of the particle size occurs.
3.2. Zeta Potential Measurements
Figure 4 shows the zeta potential as a function of surfactant concentration. For the CTAB-DNA solution, the zeta potential increases sharply from the original −22 mV to −1.45 mV with the increasing CTAB concentration from 5 μM to 100 μM. Above 100 μM, it remains to be around 0 mV with no significant changes. For the TTAB-DNA solution, the considerable change of the zeta potential occurs in the region between 50 μM and 1 mM, with an increase from −30.7 mV to −1.3 mV. For the DTAB-DNA solution, the zeta potential increases smoothly from −23.2 mV to 4.84 mV as the concentration increases from 0.5 mM to 9 mM. Surprisingly, the OTAB-DNA solution shows no significant change of the zeta potential. Although the concentration increased up to 9 mM, the zeta potential remains to be around −23 mV, which is far below 0 mV.
3.3. AFM Observation
The DLS results indicate that the surfactant-DNA particles have smaller sizes under low surfactant concentrations, while have larger ones under high surfactant concentrations. To give visual verification, AFM scanning was performed.
The representative results are shown in Figure 5. The deduced particles with smaller and larger sizes in DLS study can be found in the corresponding representative AFM characterizations. Generally, all the condensed particles except OTAB-DNA complexes exhibit typical globular conformations. The OTAB-DNA complexes have lower heights (<6 nm) compared with the conformations of other surfactant-DNA particles, as shown by the morphology analysis in Figure 5(h). This indicates that the OTAB-DNA structure is loose and OTAB cannot compact DNA with high efficiency. Moreover, the AFM scanning of the OTAB-DNA complexes suggests the coexistence of particles with large sizes and little ones, which is consistent with the bimodal size distribution in DLS measurements.
4. Discussion
4.1. The Condensing Ability of the Surfactants
The DLS results and zeta potential measurements both suggest that the compaction efficiency of surfactant increases with the surfactant chain length. To be more specific, achieving a zeta potential of ~10 mV needs the addition of ~60 μM CTAB, ~0.5 mM TTAB and ~3 mM DTAB, respectively. While for OTAB, increasing the concentration does not alter the potential significantly. AFM scanning also concludes that OTAB cannot cause a compact complex structure like other surfactants.
Though TTAB and DTAB can cause DNA compaction like CTAB, they cannot produce particles with bimodal distributed sizes. Such phenomenon may result from the high compacting efficiency of CTAB. The increase of particle size occurs at a relatively low CTAB concentration, which indicates that less surfactant molecules contribute to aggregation compared with the cases of TTAB and DTAB. Due to the lack of sufficient CTAB molecules associating with smaller-size particles, the aggregation process may stop with a considerable amount of particles do not change in their sizes. Consequently, the size distribution curves were shown to have double peaks. And in the presence of enough CTAB molecules, the bimodal distribution would disappear, as shown in the previous section.
Why OTAB cannot compact DNA into a tightly condensed structure need to be elucidated further. It is known that elastic, hydrophobic, and electrostatic factors contribute to DNA packaging. As to cationic surfactants, its hydrophobic forces and screening effects are the main terms to drive DNA to condense, while the elastic rigidity and the electrostatic repulsion between the DNA chain resist DNA compaction. The competition between the driving terms and the resisting ones determines the final conformation of DNA-surfactant complexes. If hydrophobic force overcomes the resisting terms, spherical compacted particles are the favorable conformations, which are the cases for the surfactants with sufficient long chain (CTAB, TTAB, and DTAB). For short-chain surfactants such as OTAB, the hydrophobic force is not enough to surmount the resisting ones and consequently leads to less compact structures.
The results of the present work are consistent with the observation by Husale et al. [24]. They suggest different binding modes depending on the length of the surfactant chain based on the mechanical behaviors of the DNA-surfactant complexes. Short-chain surfactants (OTAB) could lie down on the DNA surface and do not cause DNA condensation. In contrast, long-chain surfactants could have their aliphatic tails pointing away from the DNA surface, which facilitate intermolecular interactions between surfactant molecules and then induce DNA condensation. Based on the present work, it can be concluded that the association between DNA and OTAB molecules is loose and cannot compact DNA efficiently. Despite that, OTAB do change the DNA conformation according to the AFM results. With the addition of OTAB molecules, DNA molecules adopt semi-compact structures (but not densely compact structures) instead of coil conformations.
4.2. Assessment of the Aggregation at High Surfactant Concentrations and the Zeta Potential Measurements
The aggregation between the particles, both revealed by the particle size measurements and AFM characterization, can be discussed in the field of colloid science. The magnitude of the zeta potential indicates the stability of the colloidal system. In the absence of surfactants, the DNA solution used in this study has a negative zeta potential of about −35 mV. With the addition of surfactants, the charges of the DNA are neutralized step by step, along with the decrease of the absolute value of the zeta potential. When DNA molecules are compacted into particles with small sizes under low surfactant concentrations, there exist considerable negative charges on them, which can prevent other charged particles from associating. In contrast, increasing surfactant concentration decreases the amount of the charges in the particles. Consequently, the electrostatic repelling forces between them are weakened and aggregation occurs.
The size distribution and zeta potential measurements suggest that the size of a particle depends on its charges. Next, we follow the procedures described in textbook to acquire the relation [35]. The net charge of a particle with a radius of is equal and opposite to the total charge in the double layer. If the particle is deemed as spherical, its charges can be evaluated by calculating the charges of the double layer:
Substituting Poission's Equation into it gives where is the charge density, is the potential, is the distance of any point in the double layer from the center of the particle, and is the radius of the particle. The solution of the linearized Poission-Boltzmann Equation gives the relation between the potential and the zeta potential :
Combining (3) and (4) leads to the result where is the Debye-Hükel parameter and can be deemed as the "thickness" (imprecisely) of the double layer with a unit of length. In a 0.01 M solution of 1 : 1 electrolyte, has a typical value of 3.04 nm. The particle sizes in this study are generally larger than 50 nm; hence, and . If the dielectric constant of the solution and being approximately invariant in all DNA-surfactant systems, we can plot as a function of surfactant concentration to infer the variation tendency of the charges carried by the particles. Figure 6 shows the trend of with the increase of DTAB concentration. The occurrence the circled plateau reflects the approximate invariance of the charges. Such a coarse analysis seems to indicate a delicate equilibrium between the zeta potential and the particle size. In other words, the particle tends to increase its size to counterbalance the increasing screening effects of the surfactant molecules.
On the other hand, the aggregation of the particles under high compacting agent concentration might be troublesome in gene delivery, because of the increased size leading to a high steric hindrance when passing through the cell membrane. In addition, it would be more difficult to release the DNA for therapy from such particles. Therefore, it is necessary to control the size of the particles if used in this field. In comparison, the aggregation will be helpful in DNA extraction or purification, because it facilitates the sedimentation process. Based on our investigation, it is shown that measuring the zeta potential of the particles is a convenient method to judge whether they will aggregate.
5. Conclusion
The surfactant-induced DNA condensation has been shown to be dependent on the length of the surfactant's alkyl chain. Surfactants with longer alkyl chain compact DNA molecules with fewer surfactant molecules. For surfactants with sufficient long alkyl chain (CTAB, TTAB, DTAB), the particles exist with a size of 60–110 nm at low surfactant concentrations. In contrast, high concentration of surfactants leads to larger aggregates. Zeta potential measurements show that the charges carried by the particles decreases with increasing surfactant concentration. It is the reduced electrostatic repulsion that caused the large aggregates. For OTAB, the surfactant with the shortest chain in this study, it was shown to be a low-efficiency compact agent, which cannot fully neutralize the charges of DNA molecules.
Conflict of Interests
The authors declare that there is no conflict of interests regarding the publication of this paper.
This work was financially supported by the National Natural Science Foundation of China (Grant nos. 21204065, 20934004) and Zhejiang Provincial Natural Science Foundation of China (Grant no. Y4110357).
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Artist description:
just a lil something ^__^
Java Draw Created Jul 01, 2011 640 x 460px Unless noted Copyright 2011 kealani808.
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nice! col.
I love drawing dragons :3 this one is beautiful 5*'s :D hope to see more ^^
It's a shame the thumbnail isn't brilliant- this actually has a lot of detail in it that you can't see on the TN. Brilliant job 5/5 :)


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Direct admission in CHRIST UNIVERSITY
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25/11/2014 Mobiles & Accessories
Universal Superior Sim With Nano Sim Interposer + Cloud For iPhone 6,6 Plus 5C,5S,5,4S iOS 8.0(Final) to 8.0.1,8.0.2,8.1Unlocking and Activation Model: Universal Superior Sim with Nano+Cloud iPhone 6 IOS 8.0,8.1 with ALL Basebands Supported. Link: http://www.superiorsim.com/sim/index.php?route=product/product&product_id=81 Universal Superior Sim with Nano+CloudiPhone 6,6 Plus,5C,5S,5,4S IOS 8.0(Final) Unlocking and Activation Sim (iNterposer) Works with all IOS`S, ranging from iOS 7.0 to IOS 8.0 (Final),8.0.1,8.0.2,8.1 Universal Superior Sim with Nano+Cloudincludes all benefits currently developed nano-technology, as developers use the cloud to download firmware updates. Above all it has a new unlock algorithm integrated in the new Universal Superior Sim with Nano+CloudAlso update the software and interface
You can choose your cellular provider. Inserting Universal Superior Sim with Nano+Cloudmap, you will see a pop-up window asking you to "take (accept) or "reject (cancel). Regardless of your choice, the phone will receive communication. If you want to choose another operator - click "accept" and the phone will redirect you to a page with available at the moment, operators, where you make the right choice. If you do not need to change the operator, press "reject" and the phone automatically activates the operator you used last time. Three technologies in Universal Superior Sim with Nano+Cloud:
Waterproof Do not bend Antistatic
Download and update Universal Superior Sim with Nano+Cloudmaps are made from clouds at any time and in any place New Universal Superior Sim with Nano+Cloudmap created protected from this problem. Also, added copy function data cellular operator. Ability to use the original SIM card tray, eliminated the need for an unnecessary operations such as cutting or punching SIM cards. Cloud download service has been widely used. Thanks all these favorable conditions, SUPERIOR SIMShas created a brand new unlocking pattern which is Universal Superior Sim with Nano+Cloud card. We newly plan and design the system so you can experience totally different operation interface. We also simplify the operation. Iphone 6,6 Plus,5c/5s was supported to get on the internet after the jailbreak of 3G and 4G SIM. Plug in your SIM, after a while, and then you can get the signal. This card has perfectly combined using default operator with choosing a new one in an intelligent way. We add the new function that this card can restore the repetitive activation bug magically. Superior Simsalways leads the development of the whole unlocking card ( Sim Interposer) industry with the leading technology. Step 2 Putting nano-cloud unlocking card into the original card tray of iphone, then put the SIM upon the Universal Superior Sim with Nano+CloudWhen you finish according to the right order, plug in your phone and then you will seethe interface a Popup Its have 2 choice Accept or Cancel.
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Tips: When you click "Select Carrier", you will return to the situation 2, choosing operator. While clicking "Edit IMSI", you can edit international IMS code with 7 numbers. If you click "Get carrier code", these people who use the original SIM card can copy the operating codes of their operators (this function is aim to use in some small countries, specially for the remote areas), clicking "Restart SIM Card", you can restore the reactivation bug cause the American edit iphone 6,6 Plus ,5s,5c and 5 will jump to the activating page again and again. When you click this option, you can solve this problem. Choosing "Lock Carrier" means to lock the operator. Contact Details: AIM: Superior Sims ICQ: 675858855 Sonork ID:100.1651291:GSM Skype: IPHONE5DEVELOPER (USA Head Office) Skype: REPIARINGIPHONES ( Pakistan Virtual Office) Direct Call: USA Office +1-818-794-9842 Pakistan Virtual Office +923233449842 UNited Kingdom Shipping Detail :+447031890688


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This product is no longer available.
You can try these similar gifts instead.
We Have a Winner Bingo Card Stone Coasters
It can seem that bingo fanatics sometimes need a social program to stop their obsession, but if they aren't ready for an intervention they can practice on this set of 2 stone coasters that already have a game started on them.


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June 3, 2010 BY John Kim
Kevin Durant could not have asked for a better regular season. Leading his Thunder squad to 50 wins while leading the league with and astounding 30.1 points per game, Kevin Durant established himself as one of the league's elite, joining the likes LeBron James, Kobe Bryant, and Dwyane Wade. His rise to the top was largely in part due to his disciplined training regiment and committment to excellence, and that is the message he brought to Tsinghua High School during his tour of China.
A special colorway of the Nike KD II – limited to 100 pairs – was made for the Tsinghua High School basketball team to celebrate and congralate its third consecutive Chinese National Championship. This special pair features a blue upper with a black and orange trim and a translucent sole, with Chinese text outlining Durant's training sequence written under the footbed. Other special touches include KD's PPG printed on the heel through a translucent heel counter as well as encouraging messages on the tongue crest and personalized graphics on the inner tongues. Continue reading for a detailed look at this latest Nike KD II and let us know if this colorway is one of the better KD II colorways you've seen. via Dunk.
Style: 398262-401
Color: Photo Blue/Black-White-Team Orange
Filed under: Basketball Kevin Durant PE
Pages: 1 2


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(BPT) - From spices and oils to gadgets and gizmos, there are always new, trendy items to add to your kitchen. Trying to separate the handy from the hype, however, can be a time-consuming and expensive process.
Professional chefs know not everything is worthy of space in their kitchens, but it's not always easy for home cooks to figure out what they need and what they don't.
That's why Devin Alexander, New York Times Bestselling Author and professional chef, gives a peek inside her kitchen. Find out what's really worth your while as she shares the top items she uses.
Chef Alexander's top kitchen must-haves include:
Reusable oil sprayer: An oil sprayer allows you to cook with fats in a healthful and efficient way. You can fill the sprayer with your choice of oil — whatever suits your current taste preference and cooking needs.
Kitchen scale: You don't have to weigh everything, but a countertop scale is a handy tool that can help you sustain your healthy eating goals. It's great for measuring portions of lean meats, seafood and cheeses, so you can stay in check while whipping up recipes or prepping snacks.
Truvia Nectar: "My favorite liquid sweetener is blended with stevia leaf extract and honey, and has 50 percent fewer calories than sugar," says Alexander. "It's twice as sweet so you only need half as much when you're adding it to tea or drizzling it on yogurt. I also love to use Truvia Nectar in salad dressings, marinades and cocktails." Learn more at www.truvia.com/nectar.
Oatmeal: Oatmeal isn't just a morning food, but a healthy way to add texture to recipes. Use it in meatballs and baked goods for a tasty, whole-grain boost.
Cinnamon: Cinnamon is an extremely versatile pantry essential. Sprinkle the spice here and there to liven up your favorite beverages and recipes without too many added calories.
Cocoa powder: "I'm a huge chocolate lover! Using 100 percent cocoa powder, which has minimal calories and some fiber to boot, is a great way to get your chocolate fix while staying fit," Alexander says. "You can add it to oatmeal and sauces, but the possibilities are endless."
Instant coffee: Did you know instant coffee can be used for more than just your morning jump-start? It's a quick and easy way to give your protein shakes a java-flavored makeover.
Stocking your home kitchen with these essential items is an important first step toward achieving your healthy eating goals.
What do professional chefs keep in their kitchens? Devin Alexander, New York Times Bestselling Author and professional chef on NBC's "The Biggest Loser," share her top kitchen must-haves:
Oil sprayer: An oil sprayer is a useful tool that you fill with your oil of choice — and nothing else.
Kitchen scale: It's great for weighing protein and cheese so you stay in check while whipping up recipes.
Celery: It has all the crunch to satisfy your cravings and goes great with your favorite dips.
Truvia Nectar: This sweetener, blended with stevia leaf extract and honey, has 50 percent fewer calories than regular honey or sugar. Learn more at www.truvia.com/nectar.
Cinnamon: A sprinkle here and there will satisfy your sweet tooth without too many added calories. Try adding to your favorite beverages and sweet or savory recipes.


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3 Answers from Attorneys
You cannot evaluate a settlement for sexual harassment by averaging them out. First of all, settlements are always confidential. Jury or court verdicts are not. So, there is no way to know what an average "settlement" is. There is no place to look for them.
Verdicts are reported, but to get a verdict, you must go to trial and win. Even then, many verdicts are never paid, as awarded because of appeals which may overturn the verdict or a confidential settlement which may occur pending the appeal.
More importantly, one cannot generalize a sexual harassment claim. Each case is different and must be evaluated on many factors, such as the vulnerability of the victim, the egregiousness of the conduct, how severe and pervasive it was, the position of the employee in the company, the size of the company and the degree of damage or harm the harassment caused, just to name a few factors.
Experienced employment law attorneys may be able to give a rough assessment of how to value such cases, once we know all the facts, but until the case is actually litigated or mediated, even we don't know what a case will settle for.
In essence what my colleague is suggesting in his well-reasoned post is, bring your case to an experienced employment attorney for a consultation. Many attorneys might not even charge for the initial consultation, and some might even handle a good case on a contingency basis. Please don't wait to do so, though, as there are strict time limits on filing a claim and a lawsuit.
There is no 'average' or 'normal' or even any rules, guidelines or formula for determining. Every case is different. The range of value is from zero to millions, and high value is tough to achieve. It depends upon all the provable and credible facts, evidence, testimony, damages, etc. The value of the case settlement is determined by the guy that writes the check, or at trial by the jury. If this is in SoCal courts, and if you believe you have a case with merit, value and collectability, and you're serious about getting counsel to represent you, feel free to contact me. I've been doing these for over 20 years.


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Windows 7: Google Founders' Mentor Dies
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Google Founders' Mentor Dies
Techtree.com India > News > Internet > Google Founders' Mentor Rajeev Motwani Dies
Wow. Still more work ahead for him and more potential mentees.
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When President Obama criticized Citizens United two years ago in his State of the Union address, at least three justices came back at him with pitchforks and shovels. In the end, most court watchers scored it a draw. But when a comedian with a huge national platform started ridiculing the court last summer, the stakes changed completely. This is no pointy-headed deconstruction unspooling on the legal blogs. Colbert has spent the past few months making every part of Justice Anthony Kennedy's majority opinion in Citizen United look utterly ridiculous. And the court, which has no access to cameras (by its own choosing), no press arm, and no discernible comedic powers, has had to stand by and take it on the chin.
It all started when Colbert announced that, as permitted by Citizens United, he planned to form a super PAC ("Making a better tomorrow, tomorrow"). As he explained to his viewers, his hope was that "Colbert Nation could have a voice, in the form of my voice, shouted through a megaphone made of cash … the American dream. And that dream is simple. That anyone, no matter who they are, if they are determined, if they are willing to work hard enough, someday they could grow up to create a legal entity which could then receive unlimited corporate funds, which could be used to influence our elections."
Then last June, like a winking, eyebrow-wagging Mr. Smith, Colbert went to Washington and testified before the FEC, which granted him permission to launch his super PAC (over the objections of his parent company Viacom) and accept unlimited contributions from his fans so he might sway elections. (He tweeted before his FEC appearance that PAC stands for "Plastic And/Or Cash.") In recent weeks, Colbert has run several truly insane attack ads (including one accusing Mitt Romney of being a serial killer). Then, with perfect comedic pitch, Colbert handed off control of his super PAC to Jon Stewart (lampooning the FEC rules about coordination between "independent PACS" and candidates with a one-page legal document and a Vulcan mind meld). Colbert then managed to throw his support to non-candidate Herman Cain in the South Carolina primary, placing higher on the ballot than Rick Perry, Jon Huntsman, and Michele Bachmann.
The line between entertainment and the court blurred even further late last month when Colbert had former Justice John Paul Stevens on his show to discuss his dissent in Citizens United. When a 91-year-old former justice is patiently explaining to a comedian that corporations are not people, it's clear that everything about the majority opinion has been reduced to a punch line.


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Geologist Robert Young was exploring Wells Gulch in western Colorado last summer when he picked up what seemed to be an interesting rock. Some rock. It turned out to be part of a cache of what are probably the oldest dinosaur eggs ever discovered. Though now shattered, the 145 million-year-old eggs would have measured approximately 8 in. long and 3 1/2 in. across. Young, whose find was announced last week, has no idea what species of dinosaur produced the eggs, but they may shed new light on nesting habits of the prehistoric beasts. Most dinosaurs, like modern reptiles, probably laid eggs,...
Science: Dinosaur Eggs Unscrambled
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Living in a Material World: The New Information Technology
(Page 2 of 2)
what Mother Nature provided and took matters into our own hands.
—Materials science is also playing a significant role in solar power by helping companies manipulate chemical bonds to bump the rate of photonic energy capture in a given solar cell. Limiting photovoltaic cell construction to naturally occurring materials would have long ago eliminated solar power as an economically viable alternative.
—Seattle-based Modumetal is producing nanolaminate alloys that will soon replace conventional metals and composites in many applications. The company's new materials are stronger and lighter than steel, run longer and hotter than nickel alloys, and are more corrosion resistant and cost less than stainless.
The big issue seems to be making sure that new materials get the proper funding to develop. Fortunately, the federal government understands the role that materials advancements will play in determining our future economic growth and has decided to invest capital in companies and research teams with the best ideas in this space. VCs (like OVP) are also clearly on board, and are adding materials startups (like EnerG2) to their portfolios.
Another significant issue is education. To make the hard sciences the underpinning of the new energy economy will require more than just capital for entrepreneurs and business executives. It will also require a new generation of trained scientists.
It's fascinating what a difference a decade makes. During the heyday of the information technology revolution, everyone seemed to be looking for the dot-com or enterprise software company that could produce untold riches. Today, we need organic chemistry and materials science majors to mine the periodic table for the right compounds that will both leave society richer and generate new levels economic growth. Looking back, we've obviously redefined what it means to live in a material world.
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