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In hindsight, the bedazzled briefcases signaled the debacle ahead. Let's set aside the Best Picture envelope nestled inside that caused the ruckus. The topsy-turvy spectacle was triggered by these black attachès—adorned with the golden logos of the Oscars and PriceWaterhouseCooper. These swag-clad beauties were designed to draw attention to the power couple as the accounting partners sashayed down the red carpet. And therein lies the root cause of the problem. When your accountant walks the runway, leaders should run the other direction.
I am pro-influence and anti-manipulation. If you are, too, I hope you'll stand up and cheer for Tyler Schultz as you read his story. Tyler voiced his concerns about troubling practices at blood-testing start-up Theranos. So far, it has cost the now 26-year old (and his parents) $400,000 in legal fees and his once-close bond with his famous grandfather. He's living proof that influencing with integrity trumps manipulation.
The CEO firing was direct and dismissive. Barnes & Noble didn't stick to the bland yet reliable "we're moving in a different direction" script when it booted Chief Executive Officer Ron Boire. They didn't give him a gentle heave-ho with the old standby "he's stepping down to pursue different opportunities" tale. Instead, Barnes & Noble whacked their CEO in bold fashion. The core issue is trust. And trust is an endless dance.
His greatness — and crossover appeal — lived at the intersection of humanity, athleticism, and activism. Even in death, his presence is felt everywhere. Muhammad Ali was by far the most influential presence in sports. How did he shake up the world and rise from reviled to revered? And what can you do to build your own influential presence? Here are five lessons from Ali's epic presence that you can use to maximize your own greatness:
You only needed to scroll through the photos of iconic buildings bathed in purple on the days following Price's death to see evidence of his presence. From the Eiffel Tower to the Superdome, the world glowed his signature color. As we ponder his death and the outpouring of grief and tributes, I offer this thought: your presence is what remains in your absence. Not only when you leave the planet, but just as importantly to your leadership, every time you leave the room. Nearly every leader underestimates the impact that their presence has upon their ability to lead. They don't understand what presence is. They don't realize that it follows them everywhere and directly influences how whether their leadership will be successful or fail miserably. They think it's an undefined "X" factor and leave it to the rumor mill to decide how others define them. Crucial mistake. I'd like to offer five qualities of an influential presence that I've culled from Prince's legacy:
This quick read pinpoints why Mary Barra's presence is influential and what you can learn from her growth. Senior executive advisor Connie Dieken demystifies how Barra sharpened her presence and gained gravitas to deliver messages that reverberate around the globe.
It's time to think outside the in-box. Email is inefficient. It's bloated and broken. Today's in-boxes are clogged with cover-your-butt documentation, unread heads-ups and countless attempts to win eyeballs and influence decision-making. How can you break through this clutter to ensure that your e-mails are the ones that people look for, read and respond to? Follow the presence building Connect–Convey–Convince® framework to influence.
Repeat after me: "I won't spread what I've heard before confirming it." Say it loudly, three times, please. Then implement. As a young journalist, my news directors drilled into me the responsibility of accuracy, accuracy, accuracy. They made all of us reporters confirm information with three sources before we were allowed to broadcast it on TV. There was hell to pay if we aired a lie. Lives were damaged. Lawyers were called. Heads rolled. Here's the rub: everyone is a broadcaster today. Yet our social media accounts don't come with ethics training. We are not required to check sources or accuracy before we spread a fire hose of misinformation through Twitter, Facebook or any other format.
You can now witness your worst nightmare if you deliver presentations: embarrassing yourself on stage. In this case, it happened to famed film director Michael Bay – ironically, a man who directs others to deliver their best performances on camera. After an introduction exalting his superhuman status, Bay strode on stage at a Samsung event in Las Vegas. His role? Simple. To promote the company's new curved TV by reading a few prepared lines from a teleprompter. Instead, he threw everyone a curve ball by reading the wrong lines. Bay blew it and he knew it. Humiliated by his faux pas, he fled from the stage. Awkward, right?
GQ Magazine has released its list of the "25 Least Influential People." Here's the problem: The list measures the wrong thing. Before you say, "Lighten up, Connie," please consider that I get it. While I've spent the past decade as an executive coach, my previous career was in – gasp – journalism. I understand that the "Least Influential" list is meant to be provocative, even profane, to win attention in a media-saturated world. Being GQ, the list is clever and targets the interests of its male audience. But even writer Drew Magary concedes that the premise of his list is dead wrong. For example as he notes about his #6 pick, foam finger-toting Miley Cyrus, "What's sad is that it totally worked."
Dr. Martin Luther King's gravitas was cemented 50 years ago. The queen of comfort food, Paula Deen, became persona non grata in many business circles this year. I was thinking about the parallel this morning, beyond the obvious racial context. There's a leadership lesson here that's crucial to YOUR ability to have a positive, lasting impact on others. Forget the fame factor. Set aside the race aspect. This is deeply personal.
He's so chill that he has a zoned-out meme. A photo of golfer Jason Dufner slumped against a classroom wall caught fire in March. His golf game caught up this month when he won the PGA Championship – his laid-back style still on display. How did Dufner stay so cool under intense pressure to win a major championship? More important, what can YOU learn from Dufner's transformation from a guy struggling to retain his PGA Tour card to a champion hoisting the coveted Wanamaker Trophy?
The news is full of people who are full of themselves: Mayoral wanna-be and serial sexter Anthony Weiner, San Diego mayor and alleged serial pervert Bob Filner, sports legend and alleged performance enhancer Alex Rodriguez ... and former bus driver-turned abuser Ariel Castro. A bus driver? We've come to expect narcissism from high profile politicians and athletes, but a former school bus driver from a run-down home?
Who's the real deal and who's not? The mismatch between what people want us to believe and what we learn to be true can be disappointing. We are living in an almost excessively "faux" world. This post explores why it matters to you in the business world.
Think about the paradoxes in your life. You want to exude executive presence—yet still be genuine. You want to hone your speaking and influence skills—but not appear manipulative. You want to inspire top performance from others—yet not drive away top talent with your demands. You want to be—and to be seen as—the real deal. How do you balance the polarities of perfection and authenticity to lead at your peak level?
"Just be yourself." As a piece of advice, it's common—even clichéd. But it's far more demanding than it sounds, isn't it? If you're a leader, you're on the receiving end of relentless and often conflicting advice about how to present yourself to the world. Perhaps you're urged to be active on social media in order to...
As a leader, you shape the culture of your team. You have the opportunity – and responsibility – to transform a collection of individuals into a high-performing group. As much as you may want to, you know that you can't mandate teamwork. Plotters and schemers can undermine your efforts, unraveling not just your team's performance, but your customer relationships as well. The reality is that every organization is inherently a political entity. Some team members will vie for attention, resources, and positions. That's why culture is crucial – and why it begins with you.
Senator Marco Rubio chugged a tiny bottle of water during his State of the Union Republic response and instantly became comic fodder. In a swig heard 'round the world, he demonstrated why every executive must learn how to effectively communicate when Teleprompted. Lesson one: Always have water within arm's length – not off-camera. Here are a few other crucial tips I learned to master the prompter from my twenty years as a news anchor.
Membership on a senior leadership team gives you immense cachet. You're in a circle of highly visible, powerful leaders. Your role is complex. You simultaneously manage your own business unit, function, or division while serving on a senior team that creates the organization's future. To boot, you may be vying with those peers for a higher spot in the succession plan. As an executive coach, I've noticed that many of you are grappling with some loaded questions: Do I have influence with my peers? How do I add value? And how do I handle the conflicts that arise when strong-willed leaders butt heads? Here are a few ideas to ponder:
Much has been made of the interruptions during the recent Vice Presidential debate. I believe it's indicative of "communication interruptus" that's been breaking out everywhere – from boardrooms and meeting rooms to lunch rooms and chat rooms. We're living in an impatient, impulsive, instant gratification world. Interrupting, interjecting, and talking over others has become the new norm. Seems everybody wants to get a word in edgewise.sn't it frustrating to be plowed over by someone who thinks the only voice worth listening to is his own? I'd like to offer a few reasons why more people are cutting you off, how to prevent it, and how to handle a relentless, habitual interrupter.
You're the Chief Executive and your company is mired in a major public outcry. Let's call it the "Map Flap." Hiccups in your mapping software have marred your newest product release, which was first heralded by a frenzy of excitement worldwide. While you may not be Tim Cook leading Apple Inc. through an uproar, the old saying "There but for the grace of God, go I" may come to mind. Every executive can learn from Cook's corporate contrition. An effective executive apology can influence others, mitigate damage and maybe even bolster your credibility in the long run. The trick is to understand the art of the apology and follow the right steps. Here are three steps to generate goodwill through contrition...
As an executive, you're called upon to deliver important messages to your organization and the marketplace. People look to you to set the tone in public speaking. You want to come across as a strong leader, but natural. To complicate matters, your busy schedule doesn't allow a minute to spare. That's why you need to master the Teleprompter. Man and the machine are converging in today's media-centric world. If you're a leader, you're now a broadcaster. Or at least a narrowcaster with consequences. Soon, you'll be called upon to record crisp messages for both your organization's intranet and the broader Internet.
I want to share this quick video with you because it's influence in its purest form. Pure joy. Pure triumph. Pure heart. Gabby Giffords proudly reciting the Pledge of Allegiance makes me smile and tear up simultaneously. This should influence you to never give up.
As you watched the London Olympics, you may have wondered, "Why does one athlete reach her golden goal while another with equal talent falters?" Sports psychologists tell us the athlete who hauls in the hardware has learned to manage her anxiety. Same with influential leaders and presenters. After a decade of coaching executives, I've learned the key is to manage your anxiety, not try to control it. Managing and controlling are two different mindsets. If you manage your anxiety, you'll stay focused, which radically reduces your stress and improves the outcome. If you try to control it, you give the anxiety too much power. Trying to control your nerves while delivering a presentation makes you vulnerable because...
The U.S. women's gymnastics team soared, flipped and vaulted their way to the top of the medal stand at the London Olympics. They delivered the most resounding win in modern gymnastics and marked the first time a U.S. women's team has clinched gold outside of the United States. What you may not know is that these five teenagers also provided a winning template for any group who delivers presentations. Think about the last group presentation you attended. I'll bet it felt disjointed and sloppy. There was overlap or, even worse....
By now, you've heard about Olympian Ryan Lochte's face off with Michael Phelps in the 400-meter medley. You've heard how he dominated the race to win the gold. But you may be wondering, "What does a sports rivalry have to do with my presentation skills?" Everything. When you present, you are up against a competitor who's been on your tail for years. A competitor who can get inside your head and bring you down. You are competing against – YOU. Is it finally time to conquer your own demon? Let's take a lesson from Lochte with three tips you can apply right now...
Girl power, meet your new heroine. A young teen from Maine, ticked off by flagrant Photoshopping in magazines, has spun her criticism into a crusade. As a result, she's led an influential, industry-altering crusade. 14 –year old Julia Bluhm started a petition on Change.org against altered photos in Seventeen magazine. Within days, her petition had more than 84,000 supporters. Within a month, Julia and her mom were invited to New York to meet with Seventeen's editor in chief. Within reason, a commitment to change is now in place.
She's a smart cookie. Medical student Priscilla Chan inspired her famously private boyfriend, Facebook co-founder Mark Zuckerberg, to lead a public health revolution. Over dinnertime chat, Priscilla shared stories of how patients she interacted with that day were impacted by organ donations. Those who failed to find donors were dying. Those lucky enough to be on the receiving end of donations lived. Priscilla lit up like a firecracker when she shared stories about lives saved. "Why don't more people donate?" she wondered, "If only people knew about the critical shortage of organs for people who are desperately in need." That's where dinner talk turned to into action.
Let's set aside the color featured in her saucy book trilogy for a moment. The shade that's dominating the New York Times bestseller list. Literary sensation E.L. James is tickled pink. The author of Fifty Shades of Grey is simultaneously revving the engines of women and upending the publishing world. Originally a self-published author – and a first-timer, at that – James is stunned that she's influencing the way books are bought and sold. In a statement to the BBC, James calls it "an extraordinary and wholly unexpected adventure." Fifty Shades began as a free e-book, allowing for discrete buying for those who didn't want others to know they were reading erotica. After eager book club members started recommending it, the trilogy exploded and went viral. (Not to be confused with the naughty acts she writes about, of course.)
I proudly select a Harvard scholar as my Influencer of the Week. A scholar who serves as a global ambassador for YouthAIDS. A dedicated individual who has testified before the U.S. Senate Foreign Relations Committee. One who has presented to the National Press Club. A scholar who's also been lambasted by that very press this week for - wait for it - a puffy face. Ashley Judd has taken it on the chin. Make that the cheeks. If you haven't heard of the controversy, just type "Ashley Judd" into Google search and see the viral frenzy surrounding her "puffy face." You'll read elegantly descriptive words like "cow" and "pig." Who's oinking now? Ashley.
"Just do what's right," Apple's co-founder Steve Jobs reportedly advised Tim Cook, his successor as CEO of Apple Inc. That's how shift happens. Cook proved his inner boldness this week in a major shift with the late Jobs' philosophy. He announced the company's new dividend and buyback plan, essentially bringing sexy back to the musty old dividend. The quarterly check, one seen as taboo for tech companies, is now cool again.
You could hardly miss it this week. The film Kony 2012 is a triumph of marketing. It's a viral video sensation with 76 million YouTube hits and counting since its release just days ago. The Kony film shines a light on the plight of child warfare and atrocities committed by Ugandan warlord Joseph Kony. Filmmaker Jason Russell created a narrative that juxtaposes video of his young son in California with the plight of the hopeless children of Uganda. But what accounts for its influence over so many, so fast? Here's how. Russell tapped into Twitter, Facebook, high schools, colleges, and Hollywood to make it happen. While there are no silly cat tricks, there are....
A tiny town is having a huge impact, schooling the world in how to respond to a nightmare in real time, with real grace, with one heartbeat. Welcome to Chardon, Ohio. Population five thousand, give or take a few. Today there are three fewer, taken out by gunfire in the high school cafeteria. The shots rang out minutes after last Monday opening bell, when a 17 year old approached his fellow students sitting at a cafeteria table and shot them in the head from behind. He killed three and injured two others. What's remarkable about this city is its focus. Despite the sirens, SWAT teams, satellite trucks, and helicopters, they've concentrated on what matters: Relationships. Consoling. Healing.
The Oscar's Red Carpet was the center of the universe Sunday night, featuring wildly expensive couture and jewels, taut bodies and sometimes tauter faces. But turns out, fashion's biggest night was hijacked by J.C Penney. Yes, J.C. Penney. The boring one. The 110-year-old retailer used Hollywood's hottest night to reveal its own facelift. The company, now led by former Apple Inc. executive Ron Johnson, unveiled five clever ads during the Oscar telecast, featuring spokeswoman Ellen DeGeneres.
He's a point guard who's making a point, loud and clear. The New York Knicks' Jeremy Lin proves it's time to ditch two stereotypes: that Asian Americans aren't NBA material – and that brainy Harvard graduates aren't either. Lin's sudden ascent from undrafted benchwarmer to global superstar is crushing records. If you're a person whose talents have been unrecognized or under-appreciated, here are a few tips to help you make the impact you know you're capable of....
In a week that's featured the return of Adele's powerfully emotional voice, the silencing of Whitney Houston's soaring vocals, and the imminent finale of Glen Campbell's crooning to Alzheimer's disease, I feel compelled to share this this truth: Use your voice. It's the center of your influence. Ever lost your voice? I've been silenced by a case of full blown laryngitis this week and am under doctor's orders to stay completely silent for 48 hours. No talking. No whispering. Not so much as a gentle throat clearing. You'd be surprised at how much you learn about your ability to influence when you're suddenly silent – and what others imply by your stillness.
The All American linebacker with the crazy face paint – that's how Mark Herzlich was defined during his stellar football career at Boston College. Then came cancer. Doctors hoped to redefine the 2008 Atlantic Coast Conference Defensive Player of the Year as Mark Herzlich, cancer survivor. Finished with football, yet alive and well. But Mark disagreed. He had a goal. Always ambitious, he was still determined to become Mark Herzlich, NFL linebacker.
Arizona Governor Jan Brewer's tarmac tiff with President Obama influenced a major book bump. Who knew that her Scorpions for Breakfast tome even existed before the photo-op transformed it into a best-seller? Apparently not many. Brewer's book rocketed from No. 343,000 up to No. 7 in Amazon book rankings as a result of tarmac time. That's an astonishing literary leap. She transformed a digit on her right hand into a handy digital sales tool.
Wikipedia went dark for a day and illuminated the world on how to influence boldly in the 21st century. The site's massive one-day protest of the Stop Online Piracy Act (SOPA) stopped something, alright. It stopped the Hollywood-inspired bill in its tracks. The political sponsors quickly turned tail after a tribe of Wikipedia-influenced constituents weighed in. In Wikipedia's words, its supporters "shut down Congress's switchboards and melted their servers." According to the website, 162 million sets of eyeballs viewed...
2011 was a bonfire of the vanities of leaders losing their influence. From sex scandals and financial fiascos to arrogance and morality missteps, the year was packed with one powerful public demise after another. Why did these leaders fall so hard? They confused influence with manipulation. Influence is a balanced approach to changing hearts, minds and results, while manipulation is fueled by a self-centered agenda. Followers quickly turn on leaders when they feel they're being played. Here are my picks for the "Worst Influencers Alive in 2011"...
2011 separated the influencers from the manipulators. One by one, once-admired leaders from the athletic office to the corner suite to Wall Street toppled like dominoes. Turns out they lacked influence. Why? Because they misjudged what influence really means. My list of the "Top Influencers Alive" may surprise you.That's my point. Despite conventional wisdom, influence and persuasion are not the same animal. After decades of advising top leaders, I've concluded that persuasion is a self-centered skill – it's manipulation fueled by a personal agenda run amok. Influence is a balanced approach to change hearts, minds and results. There are three dimensions of a true influencer. Do you measure up?
Do you feel boring? Or fascinating? I promise that no matter how run-of-the-mill you may feel most days, you possess a leadership quality that others find fascinating. I see this unfold every day in my executive coaching practice. Every leader has a unique asset, an inner strength that defines their executive presence.
It was the silence heard around the world: Rick Perry's brain freeze. Perry's mental cramp during the GOP presidential debate stole the show and was quickly hailed as one of the worst memory meltdowns in history. It was a cringe-inducing 53 seconds as Perry scrambled to recall the name of the third federal agency he'd shut down, to no avail.
Here's a thoroughly unexpected, powerful lesson in how to be more influential. Ever fumbled around learning how to use a gadget - and taught the world an important life lesson in the process?
Increasingly, leaders like you are facing language barriers, both within your global organizations and in front of audiences in presentations. With that in mind, I'd like to share three quick lessons that I have learned.
In this edition of "Connie off the Cuff," Connie shares the truth behind what your Executive Presence is - and is not.
Tim Sanders is a confidence guru. A New York Times bestselling author, riveting keynote speaker and former Yahoo executive, Tim has written a compelling new book called Today We Are Rich: Harnessing the Power of Total Confidence that's loaded with insights on how to develop unshakable confidence in a shaky world.
Confidence is more critical now than ever in the economy we're facing today. It's essential to cultivate it so you can seize opportunities and avoid pitfalls. But it's not just recent graduates who crave confidence - every C-suite executive whom I've had the privilege to coach wants a booster shot to help them be more influential and make things happen.
In this quick 2 minute "Connie off the Cuff" video recorded behind the scenes of a keynote speech in Phoenix, you'll discover the one thing you need to deliver a presentation.
Here are some some off-the-cuff thoughts on courage and critisism from her own personal experiences.
You've been tapped to give a presentation. Quick - what are you feeling? Has a sense of dread washed over you?
Les McKeown doesn't buy into the common belief that passion and authenticity can make you influential. It's not that he's a contrarian - but this über-successful business author and serial entrepreneur pinpoints another, more specific reason for why leaders influence others.
Benjamin Zander says his job is to awaken the possibilities in others. He's a motivational speaker. And, oh, yes - he's also the conductor of the Boston Philharmonic.
Strolling along a white sandy beach on a working vacation with my teenage daughter in the Bahamas this week, we spent our mornings searching for shells.
Carol Roth is a leader you should know. Think of her as Suze Orman, one generation removed; she's a financial braintrust who leapfrogged from working class to privileged status through sheer determination and wicked smarts.
Are you an authentic, charismatic leader? Do you need to exude these qualities in order to lead effectively?
Experienced an authentic, impactful moment recently? Two families witnessed one this past weekend when my son, Spencer, proposed to his soul mate, Christine.
Pint-sized presenter Schuyler St. Leger nailed it. At the age of 10, he's already mastering audience-centered speaking.
Interesting question, isn't it? When you were in high school, influence and popularity were one and the same.
It struck me recently that you may associate influence with two things: money or charisma. You may think you need one or both to be a convincing leader who influences others and makes things happen.
When you hear "flight" and "fateful seat" in the same sentence, what comes to mind? An accident, perhaps? Allow me to explain.
An astounding thing happened to this quirky brain researcher - she suffered a massive stroke and studied herself as her brain functions shut down, one by one. In this remarkable TED Talk presentation, Dr. Jill Bolte Taylor reenacts exactly how it feels when your brain fails you.
What if you could: Overcome resistance and influence negative people who won't budge? Bypass those frustrating systems and roadblocks that give you headaches every day?
Ever received an e-mail response that struck you as the communication equivalent of Whac-A-Mole? Maybe you got a curt "see below" when you sent a question to a peer in an e-mail chain. You felt clobbered by your peer's abrupt, dismissive tone.
I recently intereviewed Dr. Condoleezza Rice in front of a live audience gathered for a book signing of her newly-released memoir, "Extraordinary, Ordinary People."
Great communicators aim to gain commitment, not just compliance. That's a key difference between being influential and being a manipulator.
Donald Trump is my new wingman. Alright, I'll be his wingman since he's a lot richer than I am. Let me explain.
Consider the fire hydrant's purpose. Your neighborhood fire plug lets firefighters tap into the municipal water system to extinguish a fire. They attach a hose to the cast iron hydrant, screw open a valve and whoosh, out comes a powerful flow of water. Simple enough.
Have a presentation to deliver? Don't follow Phil Davison's lead as a public speaker.
Being a confident communicator who influences others is a choice you make each and every day.
LeBron James and Dan Gilbert both damaged their global presence with their communications this week. LeBron's theatrics were a narcissistic nightmare. Gilbert's knee-jerk open letter was widely interpreted as an example of a sore loser.
We are all witnesses, indeed. We're all sick of witnessing the LeBron-aThon.
You might be surprised at how many executives say they lack confidence in front of audiences and want to gain the skill.
Goldman Sachs executives were skewered on Capitol Hill this week. They were sach-ed. The men faced blistering cross examination by the Senate on the firm's mortgage market and its role in the country's financial collapse.
It appeared to be an ambush worthy of the Kayne West Seal of Approval. Recently, an Academy Award winner was rudely interrupted mid-acceptance by a woman who appeared to big foot her way into his big moment.
The world's most famous athlete and a two-time presidential contender both torpedoed their careers with their self-destructive narcissism. Tiger Woods referred to his ego- maniacal state as a "sense of entitlement" in his televised mea culpa. John Edwards outed himself as a narcissist in an ABC interview after he was caught cheating on his cancer-stricken wife.
Time is of the essence when you owe someone an apology in the Internet age. As we all know, Tiger Woods certainly didn't rush to retract. By waiting 80 days to speak publicly, many people are convinced that he's arrogant and that he doesn't care.
Did you read the headlines? "Jackson Kids Steal the Show!" the news articles proclaimed, calling the appearance of Michael Jackson's two eldest children the most memorable moment of the 52nd annual Grammy Awards Show.
Kraft Chairman and CEO Irene Rosenfeld is scrambling to persuade shareholders that her company's $17 billion bid to buy British candymaker Cadbury is good for both companies. Her pursuit has drawn poor reactions from both Cadbury's shareholders and Kraft's biggest shareholder, Warren Buffett.
I spent many years anchoring the late news for an NBC-owned station, so I'm keenly aware of why general managers at NBC affiliates across America banded together, morphed into "Stone Cold" Steve Austin, and wrestled the network to the ground.
Top 10 Communi-lutions to Improve How People Respond to You In Our Distraction-Driven Decade
The balloon boy's dad, Richard Heene, thought he'd convinced America that his eccentric family should have its own reality show.
How you communicate a message has a direct impact on your ability to influence opinions.
There sure are a lot of high profile apologies floating around this week - Kayne West, Serena Williams, Rep. Joe Wilson. Which brings me to you.
What a week for high profile heckling. Let's recap:
You've been leading a high priority mission for months. You've pushed. Prodded. You've influenced internally because you believe it's the most vital issue facing your organization. Your team has invested sweat equity and they're counting on you to make it happen publicly.
You heard about the married politician caught trysting with his girlfriend in Argentina. On the day he was caught returning from his fun in the sun, South Carolina Governor Mark Sanford held a hasty, free association press conference at the statehouse to drop the bomb.
Nearly 10 million people tuned in for a record-shattering season premiere of Jon & Kate Plus 8 this week. I'll tie this to you in the workplace in a moment -- I promise there's a business communication connection.
The difference between winning and losing may come down to how people perceive the sound of your voice.
You're a smart person. The axe is falling on leaders everywhere and you want to come across as valuable and indispensable, right? Here's a quick cautionary tale to bullet-proof your credibility, straight from your TV.
Let's say you get 100 e-mails a day. (Everybody wants to "keep you in the loop.") Do you realize how much of your time that's eating up? At 3 minutes a piece, it takes you 5 hours to read and respond.
We're living in a harsh, harsh world. I'm sure you've noticed that you can now get a daily dose of cheap shots here on the Internet. Follow the comment section after most web entries and you'll find boatloads of hyper-critical, snarky comments.
Out with the old, in with the new. As we say goodbye to George Bush and his colorful, sometimes mangled communications, (can you say misunderestimated?) we usher in a new era of oratory.
News reports throughout the weekend referred to Jett Travolta as "the son of John Travolta" and called the boy's death "The John Travolta Tragedy." The boy's mother was an afterthought in the news reports, if she was even mentioned at all.
Game over. Cleveland Browns owner Randy Lerner moved swiftly to fire his front office following the final, crushing loss of the season. He cut general manager Phil Savage loose by phone after Sunday's defeat, and then sacked coach Romeo Crennel the next morning.
I feel sorry for cashiers these days. Not just because their work flow soars during the holiday rush, but because they're on the receiving end of non-stop rude encounters. One by one, customers are now yakking away on cell phones during check-out.
Today is Illinois Governor Rod Blagojevich 52nd birthday. The gift to his state? His arrest. Wiretaps show he's a profanely arrogant, self-absorbed man with a supreme sense of entitlement.
As I watched President-Elect Obama's news conference announcing his national security team nominees this morning, it reminded me of why I sat on a phone book behind the anchor desk.
It's Thanksgiving evening. You're stuffed. Bloated. Lethargic. In a turkey coma.
When I picked up my 15 year old daughter from an activity this week, I saw her standing there, waiting for me, tapping away at her cell phone. "Who were you texting?" I asked cheerfully as she slid into the car. "No one," she replied, "I was just faking it."


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Pheochromocytoma is a tumor. It is made up of special adrenal gland cells. The cells secrete hormones such as epinephrine and norepinephrine. These hormones help to regulate the heart rate and blood pressure. The cells may secrete excessive amounts of the hormones. This results in periods of:
- Very high blood pressure
- Rapid heartbeat or palpitations
- Excessive sweating
- Severe headaches
Most of these tumors are benign and grow on the adrenal glands, which are located on top of the kidneys. Other tumors occur elsewhere in the body.
In most cases, pheochromocytoma has no known cause. In others, it is caused by a genetic mutation.
Risk Factors
Factors that may increase your chance of pheochromocytoma include:
- A family history
- Tumors in other glands of the body
- Hormonal disorders
Genetic diseases associated with pheochromocytoma include:
- Von Hippel-Lindau disease
- Multiple endocrine neoplasia, type 2
- Neurofibromatosis type 1
- Paraganglioma syndromes
Most people don't have symptoms. In people who have them, pheochromocytoma may cause:
- Severe headaches
- Excessive sweating
- Fast heart rate
- Sensation of a panic attack
- Blurred vision
- Nausea, vomiting, constipation
- Pounding heart beat
- Chest pain
- Involuntary trembling
- Pain in the lower chest or upper abdomen
- Warmth, flushing
- Increased appetite
- Weight loss
- Insomnia
- High blood pressure, which can be constant or sporadic
- Tingling, burning, or numbness in the legs and feet
- Shortness of breath
- Muscle weakness
- Anxiety
- Unable to cope with high temperature
- Aggressive or unusual behavior
Your doctor will ask about your symptoms and medical history. A physical exam will be done.
Your doctor may need to test your body fluids. This can be done with:
- Urine testing
- Blood testing
- Clonidine suppression test
Your doctor may need images of your bodily structures. These can be done with:
- CT scan
- Ultrasound
- MRI scan
- MIBG scintiscan, or adrenal medullary imaging
- PET scan
Laparoscopic Adrenalectomy
If the tumor is benign, it is removed. This procedure uses small incisions and special instruments. Sometimes, the adrenal glands are removed as a part of this process.
Prior to surgery, high blood pressure will need to be brought under control using blood pressure lowering medications.
Chemotherapy and Radiation Therapy
Chemotherapy may be used if the tumor is cancerous and has spread outside of the adrenal glands.
Radiation therapy may also be used to help destroy the tumors if they have spread.
Chemotherapy may be used if the tumor is cancerous and has spread outside of the adrenal glands.
Radiation therapy may also be used to help destroy the tumors if they have spread.
Revision Information
- Reviewer: Michael Woods, MD
- Review Date: 09/2016 -
- Update Date: 05/11/2013 -

subset: commoncrawl


text:
- 1-603-942-6032
- firstname.lastname@example.com
Featured Products
Best Sellers
World Wide Chocolate
Welcome to World Wide Chocolate, an Internet only fine chocolate enterprise dedicated to providing our customers with the best imported gourmet chocolate from around the world. We serve both bulk chocolate purchases as well as singular purchases. Our number one goal is to present you, the customer, with the best service, reasonable prices and the best chocolate available online. Our website has been designed so that you can easily find artisan chocolates from truffles to hot chocolate to gourmet baking chocolate and we have product from specific countries and manufacturers. We also offer organic chocolates, specialty foods, fine chocolate gift baskets, gift certificates and wholesale chocolate!
Copyright © World Wide Chocolate All Rights Reserved
Designed & Developed by Colorado Web Solutions


text:
Vibease, the world's first wearable smart vibrator, has been named a finalist for the 2013 Crunchies Awards, a show co-hosted by GigaOM, TechCrunch and VentureBeat to celebrate the best tech accomplishments of 2013. Vibease and it's Co-Founder, Dema Tio, are Graduates of the Singapore Founder Institute.
Vibease was selected as one of five finalists in the "Can't Stop, Won't Stop Category", alongside tech giants like Netflix, Tinder, Vine, and Candy Crush Saga.
Vote for Vibease now!
Vibease has been on quite a run recently. This past summer they crushed their $15,000 fundraising goal on Indiegogo by raising $130,425, and they were featured across a ton of major media channels as well. Anthony Ha of TechCrunch wrote about them in an article titled "Crowdfunding Reaches Its Terrible, Glorious Climaz With 'Smart Vibrator' Vibease".
The Crunchies Awards Ceremony be held on Monday February 10, 2014 at 7:30 pm at the Louise M Davies Symphony Hall in San Francisco.
Learn more about Vibease at Vibease.com.


text:
If you cannot find your particular model/engine size this indicates that either the
manufacturer hasn't recommended an interval for that particular model or that it
has a timing chain and not a timing belt. Check with your manufacturer to clarify
your interval or if your vehicle has a timing chain. If your vehicle does have a timing
belt/cambelt and there is no recommended change interval in place, a decision to
change can be based upon mileage covered and/or the condition of the current belt
by a qualified mechanic.


text:
Wine, Cheese, & Chocolate
Organizer / Host
Saturday, February 16, 2013 from 7:00 PM to 9:15 PM (PST)
When Three Worlds Collide... Join LearnAboutWine for a special class bringing together three divine creations... wine, cheese and chocolate.You will explore the sensual contrasts and delights that each element brings to the experience -- the crisp acidity, mouth-filling robustness or smooth viscosity of great wine; the sharp, subtly sweet or complexity of fine cheese; and the rich luxuriousness of superior chocolate ranging from creamy milk to intensely dark.
Ticket: $80 per person
In order to purchase these tickets in installments, you'll need an Eventbrite account. Log in or sign up for a free account to continue.


text:
Iran, 1000–1400 A.D.
Learn more about the curatorial staff and the scientists and conservators who collaborate to study, exhibit, and care for the objects in The Met collection.
MetCollects celebrates works of art new to the Museum's collection through the fresh eyes of photographers and the enthusiastic voices of leading scholars and artists.
Read articles about the Museum's exhibitions, collection, and events written by hundreds of authors—including curators, conservators, librarians, digital professionals, and senior staff.
Explore publications and products inspired by The Met collection, which spans 5,000 years of culture from around the world.


text:
Registration on Wamba is closed. But you can install our different app Mamba and register there for free. Millions of users are already waiting for you there!
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By continuing, you agree with the terms of the Agreement
Post a personal
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Date mit einem Mann in Kandahar, Afghanistan


text:
The Founder Institute has helped launch over 750 new technology companies across 40 cities and five continents.
Below is a roundup of Founder Institute Graduate news over the past two weeks:
- Silicon Valley grad Udemy releases new mobile app (TechCrunch); Udemy is a leading online learning platform.
- San Diego grad CityBot was named the overall best startup in VentureBeat's #WinSXSW Contest (VentureBeat); CityBot is a mobile application that acts as an intelligent city planning guide.
- Hanoi grad Appota teamed up with Japan's Metaps to monetize apps in Vietnam (Tech In Asia); Appota is an iOS and Android app monetization and distribution platform.
- Seattle grad PetHub raises a $375k round (GeekWire); PetHub uses technology to help lost pets get home safe.
To follow Graduate News in real-time and get insights from the people building great startups, follow us on Twitter or subscribe to our weekly newsletter here.
Like this article?
Subscribe to our Weekly NewsletterAttend a Free Startup Event


text:
Use AnyLogic for simulation of:
- Supply chains and logistics
- Manufacturing and production
- Transportation and warehousing
- Airports, stations, shopping malls
- Markets and competition
- Business processes and service systems
- Healthcare
- More
Upcoming events
30 nov
02 dec
|São Paulo, Brazil
09 apr
11 apr
|Paris, France
Your feedback is much appreciated.
Simulation and modeling are important elements of a science based approach in disaster and emergency management. AnyLogic provides disaster and emergency management professionals with agent based modeling and tools that can simulate hazards and risks, emergency preparedness and response efforts, and post disaster recovery scenarios.Ali Asgary,Disaster & Emergency Management Program, York University
We found AnyLogic to be the best simulation platform on the market in terms of speed, flexibility, programability.Silviu Trebuian, Managing Director,Simando Technology SRL
We have found AnyLogic to be powerful, robust, and suitable for a wide variety of modeling projects. The ability to model using any of the major paradigms (or a combination) allows us to tailor our models appropriately. The user interface is easy to understand for people of all levels of education and experience. AnyLogic's superb technical support has helped us to model very complex systems that we would not have been able to do otherwise.Mark Kazmierczak,Gryphon Scientific
AnyLogic was the clear choice after comprehensive evaluation of multiple systems and further prototyping in those that were shortlisted. Furthermore, it continues to impress the deeper we get. There's no doubt the right simulation and modelling tool was selected for the majority of our current and future needs.Jay Ta'ala, Senior Modeller, Supply Chain Analytics,BHP Billiton Iron Ore
Multimethod modeling is the most important feature of AnyLogic. The biggest advantage for me was the capability for advanced healthcare modelling and simulation.Geoff McDonnell, Director,Adaptive Care Systems and Synergia
AnyLogic is extremely powerful, state of the art simulation software. The multimethod modeling capabilities and the ability to customize with Java are just two of the many reasons this software is so functional.Mike Prince, Senior Operations Research Specialist,BNSF Railway
I have been using Anylogic for quite some time now and have been falling in love with it. I have never seen such a powefull tool easily combing several modeling methods in a true and seemlessly integrated multimethod approach. Besides the functionality provided by the application I find the 'Get support' function very valuable. I have had a few cases where the response on my questions included a good advice or explanation and in one case the reply included the model with the suggested changes already applied. All within a few days. Excellent service helping me to quickly move on.Erwin Spekschoor, Project Manager and Consultant,Lean Six Sigma Company
AnyLogic has demonstrated the capability of going from an academic environment to helping develop realistic tools in the areas of resource optimization. The simulation framework provides the ability to use both agent-based and event-based methods to describe the stochastic behavior of machine-controlled and human-controlled objects. Furthermore, the flexibility of creating customized code and outputting multiple aspects of the simulation permits for developers to test aspects of the tools being developed. Finally, the support of AnyLogic (both in the purchasing and development phases) was tremendous.Moises Sudit, Ph.D., Director, Information Fusion Group,CUBRC
We use AnyLogic to understand better, improve and demonstrate how complex systems work. My personal favorite aspect of AnyLogic is the fact that it compiles the simulation models into a general purpose, fully object-oriented program language, like Java. Which allows us to embed optimization algorithms into simulations without writing unnecessary code.Onur Dulgeroglu, Senior Analytics Engineer,GE Global Research
The most flexible simulation modeling tool on the market - 1) through the Java platform and access to Java programming when needs for tailor-making the model appears 2) through the possibility to mix different modeling methods. Good libraries with predesigned objects and building blocks (with great flexibility in themselves), continuous improvements and enhancements in new versions, and a very good and customer-oriented support function, makes the big picture more or less complete in my view!Stefan Bengtsson,County of Stockholm
AnyLogic helps predict risks and trade offs to support decision making. It allows the users to visualize and validate plant design to gain buy in and ensure future production capacity, and forecast future workforce needs and risks. AnyLogic is very flexible, you can integrate it with many things. The software seems to evolve, and the developers listen to comments.Wenchun Annie Wang, Industrial Engineer – Simulation,CH2M Hill
The benefit of agent based modeling in AnyLogic is to truly model our supply chain. Each of the agents we use in our simulation can correspond to vessels, distribution centers, ports of loading, ports of discharge, our retailers, etc. with their own behaviors. AnyLogic makes it easy for me to give our many agents their own customizable behaviors and let them interact together in a fully integrated software.Beth Tyrie,Fruit of the Loom, Inc
AnyLogic is sufficiently flexible to support systems and policy research, yet robust enough to produce stand-alone applications that can be used by others to support their professional work.Neil McEvoy,HealthEngineer
We chose AnyLogic for three reasons, technology, support and people. I think AnyLogic has the best technology in the simulation software industry, it has the agent based, process based and system dynamics, plus it is written in Java, which means many available resources and ease of finding people who can model in AnyLogic since many know Java code. AnyLogic can be deployed on the internet, you can develop an AnyLogic model and easily share with colleagues, partners, etc.Clark Chang,Norfolk Southern Railway
TSG Consulting has been a long time user of Arena simulation software, and a couple of years back we decided that we needed a more scalable and powerful simulation tool so we surveyed the market and spent around 6 months extensively testing a large number of simulation software packages. AnyLogic was the tool that stood clear of the rest. Our consultants have adopted AnyLogic with enthusiasm and we are seeing gains in their productivity and the quality of the models they produce. To say we are pleased with AnyLogic is understating our enthusiasm for the product and as a company we look forward to a great future working with AnyLogic.Peter Bodon, Technical Director,TSG Consulting
AnyLogic has a nice object-oriented feature that allows you to create a component and reuse it, which is very useful to us, plus, the user interface has many built in charts. The most valuable feature of AnyLogic to me is to export a model, and let anyone run it on their computer without a software license.Victor Chang, Software Engineer,Intel
We were looking at the behavior of our shippers in the oil & gas industry, and we needed to model this without using hundreds of pages of code. AnyLogic was the optimal method, we could create agents, and especially dynamically create multiple agents, plus we used multimethod since there were other processes where we needed to use discrete event and system dynamics.Dumitru Cernelev, CEO,Stream Systems Ltd.
We use simulation tools, including AnyLogic, to analyze alternative capital investment solutions in order to help decide on the best investment decision. AnyLogic has helped with that decision process by providing our company with a tool that is flexible/adaptable to build models in different groups within our company using the team license server. We can easily use and combine multiple simulation methods to create the best modeling approach for each problem. The built-in functionality allows for new users to get up and running quickly and the use of Java means advanced users are able to build and customize more complex models.Jeremiah Dirnberger,CSX
AnyLogic is flexible: I don't feel hemmed in with any approach, and version 7 is a huge leap forward in the multimethod capability. We usually make changes with our eyes wide open thanks to AnyLogic!Keith Stockman, Manager of Projects and Operations Research, General Medicine Program,Monash Health
Although I am still a new user of AnyLogic, I have been very impressed with its flexibility, ease of use, and the multimethod capabilities. AnyLogic's versatility and standard libraries allow us to address a large number of different business process automation questions very quickly and with one software tool. I also believe the ability to customize ours models with Java code is invaluable when modeling the complex interactions in today's real-world systems and operations.Dr. Gregory Kott, Principal Research Scientist,PARC, A Xerox Company
I see modeling and simulation capabilities, especially agent-based modeling techniques, as critical to helping identify breakthroughs with complex phenomena associated with health care delivery. Integrating diverse types of data with multi-method simulation can advance our understanding of biological phenomena in order to deliver higher quality health care. AnyLogic's leadership in agent-based modeling, combined with its unique approach in a Java-based architecture, enables Health Services Consulting to navigate new territory and pursue cutting-edge projects now and in the future. I'm excited about the possibilities.Roger A. Edwards, ScD, Vice-President,Health Services Consulting Corporation
I've been using AnyLogic for about three or four years now, and I found that it's probably one of the better solutions out there for really being able to implement models if you don't necessarily have a very extensive coding background to start with.Joseph Simkins,Battelle Memorial Institute
AnyLogic allows to describe processes and explain them to decision makers. Its advantages are the great flexibility in reproduction of problems and the possibility to use system dynamics, agent based and discrete event simulation in one model.Romeo Placido, Director Hospital Radiology,Azienda Sanitaria Provinciale di Messina
AnyLogic is a tool where a modeler can implement any of his ideas. Easily, fast and with enjoyment. A special thanks to the AnyLogic documentation and Support Team! Their perfect work makes your induction into AL world smooth and happy!Yuriy Pidvalny, ML-IS,Kuehne + Nagel
We chose AnyLogic because we found that traditional techniques did not do just to what we were trying to model. When we saw AnyLogic, it was a great ah-ha moment to say this is the tool I need to solve the problems I am trying to solve. The benefits of a multi-method modeling approach are that you can solve the problem more naturally. If you do not have the ability to do multi-method modeling, you end up creating all types of anomalies in your model, fitting the methodology rather than fitting the problem. Multimethod modeling is a great effective tool.Bipin Chadha, Ph.D., Data Scientist,USAA
I have seen a lot of simulation products and this is the only one that can combine all three methodologies. Plus, the ability to show all the graphic components has a great impact on who I present the models to. This allows AnyLogic to be THE choice for simulation modeling.Kelvin Blyden, Supply Chain Analyst,Campbell's
AnyLogic is proved to be a very useful tool as I can build simulations using different simulation methodologies and create intuitive and appealing interfaces for end-users. Most functionality is available as predefined objects but it is nice to have the flexibility to add your own structures if necessary. Their technical support team is very knowledgeable and responsive; their support has been outstanding in my experience.Principal Informatics Scientist,AstraZeneca Pharmaceuticals
We chose AnyLogic because of its flexibility. We have many customers in many industries that have very different requirements because their supply chains are different, and we've been very easily able to change our models quickly to interact with the customers. The agent based tool is very powerful for us, our automated robots are highly intelligent, so we want to represent those behaviors using the agent based approach inside the AnyLogic tool, and we have very large fleets, up to 500 robots in a system, and AnyLogic is able to handle the complexity of the interactions amongst those 500 robots and able to do computations efficiently.Dr. Larry M. Sweet, CTO,Symbotic LLC
AnyLogic is an easy to learn platform that is able to incorporate various modeling techniques to allow us to create powerful solutions for our clients.Eric Rosenbaum, Experienced Associate,PricewaterhouseCoopers LLP
AnyLogic has been perfect for us because of its flexibility and multimethod approach. We are able to look at a lot of different types of interactions, that you couldn't typically do with, for example, a discrete modeling software. We are able to address many different problems with just one tool, which is fantastic for us.Allan Chegus, President,Stream Systems Ltd.
AnyLogic is a powerful simulation tool combined with Java. What I like about AnyLogic is its velocity, adaptability, and versatility.Mariana Dorigatti,INGAR-CONICET
I would recommend AnyLogic software because it is a mature platform that allows a combination of multimethod modeling paradigms, in a very user friendly and open manner. Plus it takes advantage of Java which is also a technically mature and secure way of building models. The advantages of agent-based modeling with AnyLogic is that you can combine it with other methods and combine existing knowledge with agent based approach. Also, it's fairly user friendly, the facilities in the graphical user interface and development are very good.Artem Parakhine,PricewaterhouseCoopers LLP
I am new to the AnyLogic product, but have gotten up to speed quickly. In part, this reflects the strength of the product itself - the ability to design the simulation logic graphically, using the extensive set of tools provided. However, the AnyLogic staff has also played a large role, through training and support. The technical team even added custom features to the language to better support my specific needs.Roger Baugher,TrAnalytics LLC
One of the most important AnyLogic features is the ability to build our own libraries. We only have to build them once, but can use them multiple times in different models.Gunnar Feldmann, Assistant Operations Research Specialist,Norfolk Southern Corporation
Anylogic is by far one of the most advanced software programs for simulation of complex systems. It can be applied efficiently in the modeling of highly complex systems in various spheres of contemporary economics and teaching, as well as in teaching MBA classes to top managers and corporate decision makers.Dmitry Katalevsky, Ph.D. Economics, lecturer MBA/EMBA classes, Senior Manager,Deloitte & Touche CIS
AnyLogic support team has been very proactive in supporting my projects and meeting my deadlines. I've been working with AnyLogic software less than a month and I'm feeling confident moving forward as I continuously learn a lot from the support team. AnyLogic support team to me is brilliant, fast, energetic and fully equipped with technical knowledge. Fear no more as anyone can easily learn simulation with AnyLogic's perfect support team guidance and user-friendly software.Ching-synn Teo,AMD (Advanced Micro Devices, Inc.)
AnyLogic provides simulation modelling that steps outside the limitations of many traditional evaluation techniques. Which means, when used correctly, it gets around the biases inherent in old school thinking. If you want to find new and innovative ways of doing business, understanding issues, developing policy then you need to have evaluation tools that can keep up. AnyLogic provides that flexibility.Gerard Nelson,RedHot Innovation
We've been using AnyLogic probably for four years now. Most of my work time I was working with another academically based agent based modeling system. We were aware that AnyLogic existed, but only after a while did we think that maybe we should look at it instead of looking at the system we were using, and we found that it was easier for us to learn and to implement.Neil McEvoy, Director,Centre for Research in Healthcare Engineering
We chose AnyLogic to tackle our large complex problem because of the multimethod models you can use, the mix of agent based, discrete event and system dynamics is a very useful combination. My favorite part of AnyLogic is all the dashboard features, the great charts and business intelligence you can get from the agents that are working in the model.Kyle Johnson, Global Business Services, Advanced Analytics and Optimization,IBM
I go back with AnyLogic to the very beginning: over ten years now. Ten years ago, AnyLogic was what I call the only industrial strength product that had a hope of doing both system dynamics, agent based, and discrete event in one package. As best as I can tell, it's still the only package that has that capability. As we do more especially in the realm of agent based models, there are some open-source tools, but none of them has anything close to the functionality that AnyLogic does.Dr. Mark Paich, Director,PricewaterhouseCoopers LLP
AnyLogic is a simulation package that provides value to users at all levels. The visual UI allows entry level users to quickly develop insightful simulation models. The ability to customize models with Java provides the flexibility and freedom for advanced users to develop highly detailed and complex models efficiently. The integration of AnyLogic with such a widely known language such as Java makes it accessible to a very broad talent pool, and eases the learning curve for good programmers to become advanced AnyLogic users.Paul Corry, Lead Simulation Analyst,Aurecon Australia
The competition is constantly getting tougher, and today you have to compete for a one-hundredth of a percent of a market share in many industries. That's why AnyLogic is unique. It allows you to optimize business processes and rise your resource utilization rate up to the level unachievable for most of the competitors.Alexey Mashanov, CTO,Magnat Group
Many specialists take part in plant planning process today, with everybody having their own vision, which has to be considered. AnyLogic is the best tool both for getting the operational data about the future system and an opportunity to visualize the work of it. This allows all the project participants to concretize and detalise their perceptions of how future plant has to be designed, and as a result, avoid mistakes while implementing the project. When huge long-term investments are involved, avoiding bottlenecks allows to save a lot of money during the years of plant operation, and AnyLogic is the way to help us in it.Nikita Shchedrov, Project Engineer,Begarat LLC
We chose AnyLogic because of the following features: flexible pedestrian modeling abilities, intuitive user interface, simplicity of programming, visualization abilities, and the ability to gather a wide range of statistical data for analysis. During our work with the AnyLogic team, we appreciated the efficiency and the quality of the technical support, the expertise of the company engineers, and the willingness of the company to improve the product for the clients.Alexey Pashkevitch,ITS Consulting
AnyLogic offers wide analysis and business process optimization capabilities in comparison to the traditional analytical methods. Support of multiple methods allows the analyst to choose solutions relevant to the goals of simulation and the available statistics. Other important advantages of the system are the optimization module and GIS map integration.Nikolay Begunov, PhD, Project Manager,Ural Bank for Reconstruction and Development
AnyLogic is an extremely powerful software to create complex simulation models. We haven been working on Rough Cut Capacity model, with a large number of SKU`s and production lines, and the diversity of AnyLogic features and methods enable us to model all complex situations along the production process. All along our development we have received an outstanding support, always in time and very clear. The support team even developed some custom examples to fulfil our needs. We feel AnyLogic is a great tool with an excellent support team.Elisa Elena,ITE-CONSULT
AnyLogic is both a user-friendly graphical environment and a multi-functional set of libraries for building simulation models. An ability to customize models with Java grants professional modelers with unlimited capabilities for problem-solving in any practical area.Ruslan Sukhih,Solver Robotics LLC


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All options are on the table.
This is what Gov. Bill Richardson's deputy chief of staff, Bill Perlman, reportedly told the "high-powered" College of Santa Fe Task Force.
All options except that of a focused and progressive art school working toward a national reputation that would lend meat to Santa Fe's somewhat spurious claim of being a significant art center. That was, after all, the College of Santa Fe's most recent mission before its pretense of being a college at all—let alone one with a commanding sense of purpose—was demolished by a lazy, ineffective board and a succession of presidents more interested in padding their résumés than invigorating the troubled school.
At this point, all the options on the table appear to water down the school's focus on the arts—in fact, it's only lucky happenstance that a school remains at all. What's on the table is allowing private developers to create a retirement community.
Forget the much-trumpeted need to stop the attrition of young people, and to attract entrepreneurial spirit, creative class pioneers and the kind of healthy, youthful people who invigorate a city's coffers and bring business with them in their pursuit of "quality of life. When real money is on the table, the powers that be are all ears for short-term economics and increasing Santa Fe's ever-swelling fogy factor.
Forget about the Santa Fe Art Institute, which leases its facilities from the College of Santa Fe; it appears to be mysteriously unrepresented on the CSF Task Force, let alone mentioned by the media. Host of one of Santa Fe's very few residency programs, and the arts organization most acutely operating at the intersection of art, politics and cultural criticism, the Institute offers the rare gift of proactive programming with a conscience.
Pay no attention, please, to the accolades received by members of CSF's creative writing program, just in the last year, from the New York Times (including Robin Romm landing the cover of the Sunday Book Review)—feats unmatched as far as I know, by any other anything in the entire state.
Disregard, please, that the arts and culture industry is the most significant non-governmental factor in Santa Fe's economy, providing the bulk of revenue, the bulk of jobs and the bulk of the entire region's tourist allure—so recently categorized as cultural tourism.
Instead, let's all focus on how to water down the school and ensure that every numbskull with the governor's phone number and an opinion can carve out a slice of what happens to a prime piece of Santa Fe's physical, intellectual and creative real estate. Back when New Mexico Highlands University was going to be the knight in shining armor, we had to inure ourselves to the prospect that CSF could host Highland's claim to fame: its "education" program—a degree so illustrious that it graces the wall of every middling, tyrannical, status quo school administrator in New Mexico.
Now Santa Fe Community College President Sheila Ortego—not a numbskull—has designed a plan to have CSF become a state "learning center." Under Ortego's plan, CSF would become a kind of educational tasting platter with hors d'ouevres served up from all of the state's educational stakeholders. Ortego helms a responsible, effective educational institution that has become a linchpin in the Santa Fe community, and her plan is smart, proactive and logical. It includes everyone, offends no one—at least no one on the task force—and could be viable under any number of variations. But rarely is the most practical and politically expedient solution the best one.
What we need now is courage, especially after lawmakers were too cowardly to play it straight with students who dedicated so much time and energy to a bill to save their school. The courage needs to begin with the CSF Board of Trustees accepting its fiduciary responsibility. I've yet to accurately gauge the cumulative wealth of the board, but even given that it might be half of what it was a year ago, I know it's plenty. The Peters and Zeckendorf families alone could each write a check for $25 million ($50 million total—just to make sure the debt is covered and then some) and never even feel the hiccup in their bank accounts. We also know that the Peters and Zeckendorfs both enjoy social prominence in Santa Fe because of the arts. History can assign the role of hero or villain, and these are people with the power to choose for themselves and set the table however they like.
Stepping up and solving the problem that occurred on their watch would allow the College of Santa Fe to become something different, distinct and fitting for the future of the city, rather than a mishmash of compromises. After all, "Big Bill" Zeckendorf—originator of the New York-based development fortune—is famous for once saying "to do less than the ultimate is to do nothing."
All options are on the table.


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WRIGHT: Luke makes ICC World Twenty20 Final Squad21-Aug-2012
Sussex's Luke Wright has been named in the final 15-man England Squad for their defence of the ICC World Twenty20 in Sri Lanka in September.
Luke Wright been in fantastic form for Sussex Sharks who have made it into Final's Day of the Friends Life t20.
England are current holders of the ICC World Twenty20 title and will face Afghanistan and India in the group stages beginning on Friday 21 September against Afghanistan in Colombo. The full 15-man squad is as followed:
Stuart Broad (Nottinghamshire) (captain)
Jonny Bairstow (Yorkshire)
Ravi Bopara (Essex)
Tim Bresnan (Yorkshire)
Danny Briggs (Hampshire)
Jos Buttler (Somerset)
Jade Dernbach (Surrey)
Steven Finn (Middlesex)
Alex Hales (Nottinghamshire)
Craig Kieswetter (Somerset)
Michael Lumb (Nottinghamshire)
Eoin Morgan (Middlesex)
Samit Patel (Nottinghamshire)
Graeme Swann (Nottinghamshire)
Luke Wright (Sussex)


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Rush processing speeds the processing time in our warehouses only. You may still need to use expedited shipping methods to receive your order in time. If you have any questions, please contact us.
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Bringing a school back from the brinkOctober 17, 2013
In 2007, the smallest school in a rural Kansas school district was facing closure. Today this same school is a project-based learning charter elementary school – complete with a teaching barn, greenhouse and wind turbine.
The leaders of the school's transformation will speak in Emporia later this month about how Walton Elementary went from an elementary school struggling to remain open to become the Walton 21st Century Rural Life Center. Regarded as a model for struggling rural communities, the school has succeeded beyond the most optimistic vision of those involved with the project.
Dr. John Morton, former superintendent for the Walton school district and now an associate professor in ESU's School Leadership/Middle and Secondary Teacher Education department, will lead the presentation about the school's metamorphosis. He will be joined by Natise Vogt, Principal of the Walton 21st Century Rural Life Center, and Derrick Richling, an Emporia State University graduate and teacher at Walton.
The lecture is free and open to the public. It will be held on Wednesday, Oct. 30, from 3:30-4:30 p.m. in Visser Hall, Room 330 on the Emporia State University campus. The presentation is part of The Teachers College Speaker Series and is sponsored by the Flint Hills Chapter of Phi Delta Kappa.
A live webcast of the lecture will be available via Adobe Connect at https://connect.emporia.edu/tc/.
Morton was recently accepted to speak about the transformation of the school at the Hawaii International Conference on Education in January. The annual conference draws more than 1,000 participants from over 35 countries. The goal of the conference is to provide an opportunity for academicians and professionals from various education related fields from all over the world to come together and learn from each other.
Check out our students' achievements and accomplishments on Emporia State's meritpages.com site


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A stress fracture is a tiny crack in the bone from chronic overuse. Most stress fractures occur in the lower leg and foot. They can also occur in the hip and other areas.
A blow to the bone does not cause a stress fracture. Rather, it is typically caused by repeated stress or overuse. Some causes are:
- Increasing the amount or intensity of an activity too quickly (most common)
- Switching to a different playing or running surface
- Wearing improper or old shoes
Stress fractures can worsen by continued physical stress. Smoking can also make stress fractures worse because it interferes with bone healing.
Risk Factors
Stress fractures are more common in women. Other factors that may increase your chance of a stress fracture include:
- Sports that involve running and jumping, such as:
- Tennis
- Track, especially distance running
- Gymnastics
- Dance
- Basketball
- Absence or early stopping of menstrual cycle—amenorrhea
- Reduced bone thickness or density—osteoporosis
- Poor muscle strength or flexibility
- Overweight or underweight
- Poor physical condition
A stress fracture may cause:
- Localized pain on the bone
- Pain when pressure is applied directly over the fracture and the area around it
- Pain when putting stress on the affected leg
- Swelling and warmth at injury site
You will be asked about your symptoms and medical history. The injured area will be examined for localized pain and swelling.
Imaging tests to evaluate your bones include:
Treatment includes:
Nonsteroidal anti-inflammatory drugs (NSAIDs) can relieve pain, but controversy exists about their use for stress fractures. It is possible that NSAIDs adversely affect stress fracture healing.
Rest is the most important thing you can do for a stress fracture. This includes avoiding the activity that caused the fracture and any other activities that cause pain. Rest time required is at least 6-8 weeks.
Shoe Inserts or Braces
Shock absorbing shoe inserts and pneumatic braces may provide comfort and quicken recovery.
Crutches or a Cane
You may need crutches or a walking cane to keep pressure off the leg.
Talk with your doctor about when you can restart activity and how to progress with the amount and type of activity.
To help reduce your chance of a stress fracture:
- Gradually increase the amount and intensity of an activity
- Run on a softer surface, such as grass, dirt, or certain outdoor tracks
- Do not overdo any activity
- Wear proper footwear
- Maintain a proper weight
- Avoid smoking
- Eat a healthy diet rich in calcium and vitamin D.
Revision Information
- Reviewer: Michael Woods, MD, FAAP
- Review Date: 09/2016 -
- Update Date: 09/30/2013 -


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1 Joe West
This is the story of Joe West: the guitar slingin' local hero who takes your preconceived notions of country, folk, gospel and rock, and turns them upside down. No longer will we be forced to lump in country and folk with toothless weirdos or faux cowboys; West is for real, and he is more handsome than you.
West is a beloved fixture and institution in our scene, whether it's The Joe West Show, The Santa Fe All-Stars or the ongoing saga of Xoe Fitzgerald, the Time Traveling Transvestite. Whatever the band and whatever the style, one thing remains constant: West owns you! Big plans are in the works for more chapters in the tale of Xoe Fitzgerald, some Santa Fe All-Stars performances, as well as a variety-style radio show—not even to mention his myriad solo appearances, gospel brunches and whatever other amazing-ass stuff he's got hidden up his sleeve.
West has lived and performed all over the country, most notably in New York and Austin, yet he holds Santa Fe very near and dear to his heart. "There is such an incredible pocket of talent in this town," he says. "It's hard to stay away." True, my friend. And forgive me asking you this favor: Don't go away…or I will cry.
2 Tony C'de Baca
3 Jono Manson
1 Joe West


text: The brightest lights of this big city are found in the Broadway - Times Square neighborhood. Experience live entertainment, such as musicals and plays, or tour Rockefeller Center. The iconic St. Patrick's Cathedral graces the entire block of Fifth and 50th and welcomes more than 5 million visitors each year. This thriving area filled with pedestrians also hosts NBC, ABC, MTV, and NASDAQ.Hide


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It looks like your browser is out of date.
To use all features of KLM.com safely, we recommend that you update your browser, or that you choose a different one. Continuing with this version may result in parts of the website not being displayed properly, if at all. Also, the security of your personal information is better safeguarded with an updated browser.
Red telephone boxes and black cabs characterise London's city streets. Book flights to London and you'll return home with a mind full of cultural experiences and the very latest trends in your shopping bags. Visit Buckingham Palace and meet the Beefeaters at the Tower of London. A stroll through Hyde Park is a wonderful way to begin the day. And if you find yourself in the neighbourhood, pop into Harrods and marvel at the couture and sparkling diamonds. Never a dull moment in London!
Whether you enjoy peering into the windows of the most expensive shops or are on the lookout for a bargain, everyone's a winner in London. Bustling Oxford Street is full of big department stores and shopping chains. If you prefer one-off boutiques or antique shops, just hop in a taxi and head for the colourful streets of Notting Hill. If history is your thing, you can't afford to miss the imposing Tower of London. Take a stroll the iconic Tower Bridge and you're virtually there. London is the ideal destination for a varied city trip and the perfect starting point for a longer holiday in England. Treat yourself and book a flight to London today!
You could easily spend an entire weekend visiting London's many museums. The British Museum is worth a visit for its amazing architecture alone. For modern art, the place to be is the Tate Modern on the South Bank of the Thames. The South Bank is also home to the London Eye, whose glass capsules offer glorious views of the city. And when Big Ben strikes 5 o'clock, the London pubs fill up: it's time for a pint!
Looking for a cheap flight to London? With KLM, your holiday begins the moment you step onboard. Your journey will fly by with your own entertainment set on intercontinental flights: free films, games and music. Enjoy our selection of snacks or a tasty dinner and drinks. Add in our many handy extra services (some available for a supplement) and you'll be in the UK before you know it.
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'Brightest star' Williams honoured
Billy Crystal has paid tribute to his pal and comedy brother Robin Williams at the Emmy Awards, movingly remembering him as "the greatest friend you could ever imagine".
Following the ceremony's in memoriam segment, Billy appeared on stage at Los Angeles' Nokia Theatre to honour Robin, who was found hanged in his Northern California home on August 11. A luminous image of the late comedian hovered overhead.
"He made us laugh, big time," said Billy.
After Robin's apparent suicide, Billy simply wrote on Twitter of his long-time friend and Comic Relief co-host: "No words."
But he found moving words to remember Robin at the Emmys, recalling family gatherings, ball games and nights on stage together.
"I used to think if I could put a saddle on him and stay on him for eight seconds, I was going to do OK," said Billy.
"It's very hard to talk about him in the past because he was so present in all of our lives. He was the brightest star in the comedy galaxy."
Billy concluded by reflecting on the eternal glow Robin would continue to give, long after he was gone.
"And you'll think to yourself, 'Robin Williams, what a concept'," he said.


text:
ŚáļÁČąśó•śúü: 2010-03-10
šĹúŤÄÖÔľö šļļť°ěśôļŚļęÁ∑®ŤľĮťÉ®
Ť£ĚŤ®āÔľö ŚĻ≥Ť£Ě.ŚĖģŤČ≤ŚćįŚą∑.24ť†Ā.
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Credit Cards (Mastercard & Visa)
We currently accept Mastercard and Visa credit cards. Your credit card information is secured and safeguarded when shopping at Youbeli.com. All information are encrypted and sent through secured and protected channels. As we are employing Mobile88 (iPay88) as our payment gateway, Mobile88 will be stated in your credit card statement.
Financial Process Exchange (FPX)
We also accepts FPX payment method as it is an ideal payment solution for customers who does not have a credit card. All that is needed to make an online payment is an online banking account. Payment is directly linked and connected to the customer's specified online banking account, transactions are usually instant and fast. Supported banks includes:
Cash Bank-in / Online Fund Transfer / Cheque
You may bank-in cash or cheque into the selected bank account below:
Bank : PUBLIC BANK
Account Name : YOUBUY ONLINE SDN BHD
Account No. : 3194858935
Bank : MAYBANK
Account Name : YOUBUY ONLINE SDN BHD
Account No. : 514048612629
Once you have successfully bank-in the amount, email the bank in slip to firstname.lastname@example.com with the stated Order No.
PayPal lets you send payments quickly and securely online using a credit card or bank account.
Not valid for cash only items.
|Delivery Fee (RM)
|West Malaysia
|First 2.00 kg
|West Malaysia
|Additional 1.00 kg
|East Malaysia
|First 1.00 kg
|East Malaysia
|Additional 1.00 kg
|First 1.00 kg
|Additional 1.00 kg
|Order Price Range (RM)
|Delivery Fee (RM)
|West Malaysia
|Above 39.00
|East Malaysia
|Above 79.00


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Note : Your Quotation Request will be directed to L-3 Narda-ATM.
The AF085X-20 from L-3 Narda-ATM is a RF Variable Attenuator with Frequency 7.2 to 8.4 GHz, Attenuation Range 20 dB, Power 7 W, Peak Power 3 kW, Insertion Loss 0.5 dB. Tags: Continuously Variable, Rotary. More details for AF085X-20 can be seen below.
DC to 26.5 GHz Programmable Step Attenuator with 1 dB Steps
You can now find similar products from multiple companies on everything RF.


text:
Pregabalin/Thiazolidinedione Antidiabetics
This information is generalized and not intended as specific medical advice. Consult your healthcare professional before taking or discontinuing any drug or commencing any course of treatment.
Medical warning:
Moderate. These medicines may cause some risk when taken together. Contact your healthcare professional (e.g. doctor or pharmacist) for more information.
How the interaction occurs:
Both medicines can fluid to build up in your body. The extra fluid can cause stress on your heart.
What might happen:
Some patients who take these medicines together may develop heart failure. In some patients who already have heart failure, their heart failure may get worse.
What you should do about this interaction:
Let your healthcare professionals (e.g. doctor or pharmacist) know that you are taking these medicines together. If your doctor decides that using these medicines together is the best therapy for you, you will be monitored. Let your doctor know if you notice any swelling, sudden weight gain, trouble breathing, or change in your condition.Your healthcare professionals may already be aware of this interaction and may be monitoring you for it. Do not start, stop, or change the dosage of any medicine before checking with them first.
1.Lyrica (pregabalin) US prescribing information. Pfizer, Inc. August, 2011. .


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Upstream / Downstream
Explore pathways related to this product.
To Purchase # 2965S
|100 µl (10 western blots)
|300 µl (30 western blots)
Find answers on our FAQs page.
PhosphoSitePlus® Resource
- Additional protein information
- Analytical tools
Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965
Western blot analysis of extracts from NIH/3T3 and Jurkat cells, untreated, PDGF-treated or LY294002-treated as indicated, using Phospho-Akt (Thr308) (C31E5E) Rabbit mAb (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower).Learn more about how we get our images
Gallery: Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965
Western Blotting Protocol
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
A. Solutions and Reagents
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
- 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
- 10X Tris Buffered Saline (TBS): (#12498) To prepare 1 L 1X TBS: add 100 ml 10X to 900 ml dH2O, mix.
- 1X SDS Sample Buffer: Blue Loading Pack (#7722) or Red Loading Pack (#7723) Prepare fresh 3X reducing loading buffer by adding 1/10 volume 30X DTT to 1 volume of 3X SDS loading buffer. Dilute to 1X with dH2O.
- 10X Tris-Glycine SDS Running Buffer: (#4050) To prepare 1 L 1X running buffer: add 100 ml 10X running buffer to 900 ml dH2O, mix.
- 10X Tris-Glycine Transfer Buffer: (#12539) To prepare 1 L 1X Transfer Buffer: add 100 ml 10X Transfer Buffer to 200 ml methanol + 700 ml dH2O, mix.
- 10X Tris Buffered Saline with Tween® 20 (TBST): (#9997) To prepare 1 L 1X TBST: add 100 ml 10X TBST to 900 ml dH2O, mix.
- Nonfat Dry Milk: (#9999).
- Blocking Buffer: 1X TBST with 5% w/v nonfat dry milk; for 150 ml, add 7.5 g nonfat dry milk to 150 ml 1X TBST and mix well.
- Wash Buffer: (#9997) 1X TBST.
- Bovine Serum Albumin (BSA): (#9998).
- Primary Antibody Dilution Buffer: 1X TBST with 5% BSA; for 20 ml, add 1.0 g BSA to 20 ml 1X TBST and mix well.
- Biotinylated Protein Ladder Detection Pack: (#7727).
- Prestained Protein Marker, Broad Range (11-190 kDa): (#13953).
- Blotting Membrane and Paper: (#12369) This protocol has been optimized for nitrocellulose membranes. Pore size 0.2 µm is generally recommended.
- Secondary Antibody Conjugated to HRP: Anti-rabbit IgG, HRP-linked Antibody (#7074).
- Detection Reagent: SignalFire™ ECL Reagent (#6883).
B. Protein Blotting
A general protocol for sample preparation.
- Treat cells by adding fresh media containing regulator for desired time.
- Aspirate media from cultures; wash cells with 1X PBS; aspirate.
- Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice.
- Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).
- Heat a 20 µl sample to 95–100°C for 5 min; cool on ice.
- Microcentrifuge for 5 min.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#13953, 5 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
- Electrotransfer to nitrocellulose membrane (#12369).
C. Membrane Blocking and Antibody Incubations
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
I. Membrane Blocking
- (Optional) After transfer, wash nitrocellulose membrane with 25 ml TBS for 5 min at room temperature.
- Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
II. Primary Antibody Incubation
- Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the product datasheet) in 10 ml primary antibody dilution buffer with gentle agitation overnight at 4°C.
- Wash three times for 5 min each with 15 ml of TBST.
- Incubate membrane with Anti-rabbit IgG, HRP-linked Antibody (#7074 at 1:2000) and anti-biotin, HRP-linked Antibody (#7075 at 1:1000–1:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
- Proceed with detection (Section D).
D. Detection of Proteins
Directions for Use:
- Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST.
- Prepare 1X SignalFire™ ECL Reagent (#6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.
- Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Western Blot Reprobing Protocol
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
- Wash Buffer: Tris Buffered Saline with Tween® 20 (TBST-10X) (#9997)
- Stripping Buffer: To prepare 100 ml, mix 6.25 ml of 1M Tris-HCl pH 6.8, 10 ml of 20% SDS and 700 μl β-mercaptoethanol. Bring to 100 ml with deionized H20. Make buffer fresh just prior to use.
B. Protocol
- After film exposure, wash membrane four times for 5 min each in TBST. Best results are obtained if the membrane is not allowed to dry.
- Incubate membrane for 30 min at 50°C in stripping buffer (with slight agitation).
- Wash membrane six times for 5 min each in TBST.
- (Optional) To assure that the original signal is removed, wash membrane twice for 5 min each with 10 ml of TBST. Incubate membrane with LumiGLO® with gentle agitation for 1 min at room temperature. Drain membrane of excess developing solution. Do not let dry. Wrap in plastic wrap and expose to x-ray film.
- Wash membrane again four times for 5 min each in TBST.
- The membrane is now ready to reuse. Start detection at the "Membrane Blocking and Antibody Incubations" step in the Western Immunoblotting Protocol.
posted June 2005
revised October 2016
|Western Blotting
Phospho-Akt (Thr308) (C31E5E) Rabbit mAb detects endogenous levels of Akt only when phosphorylated at Thr308.Species Reactivity: Human, Mouse, Rat, Hamster, Monkey
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr308 of mouse Akt.
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).
1. Franke, T.F. et al. (1997) Cell 88, 435-7.
2. Burgering, B.M. and Coffer, P.J. (1995) Nature 376, 599-602.
3. Franke, T.F. et al. (1995) Cell 81, 727-36.
4. Alessi, D.R. et al. (1996) EMBO J 15, 6541-51.
5. Sarbassov, D.D. et al. (2005) Science 307, 1098-101.
6. Jacinto, E. et al. (2006) Cell 127, 125-37.
7. Cardone, M.H. et al. (1998) Science 282, 1318-21.
8. Brunet, A. et al. (1999) Cell 96, 857-68.
9. Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-4.
10. Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5.
11. Vlahos, C.J. et al. (1994) J Biol Chem 269, 5241-8.
12. Hajduch, E. et al. (2001) FEBS Lett 492, 199-203.
13. Cross, D.A. et al. (1995) Nature 378, 785-9.
14. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.
15. Gesbert, F. et al. (2000) J Biol Chem 275, 39223-30.
16. Zhou, B.P. et al. (2001) Nat Cell Biol 3, 245-52.
17. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
18. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. DRAQ5 is a registered trademark of Biostatus Limited. U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.


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Not all risk factors for asthma can be prevented. However, environmental factors can be changed.
Current evidence for asthma prevention supports eliminating maternal smoking during pregnancy. There is also concern that if pregnant mothers breathe second-hand smoke, their children may also be at increased risk for asthma.
There is some evidence that reducing exposure to common allergy producing substances during pregnancy and during the first 2 years of life might reduce the risk of developing asthma.
While we do not know for sure that taking acetaminophen during pregnancy increases asthma risk, pregnant women should discuss this and other risk factors with their doctors.
Revision Information
- Reviewer: Michael Woods, MD
- Review Date: 09/2016 -
- Update Date: 08/14/2015 -


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Full Details
Keep your hands dry as you maintain your tank and rearrange your plants with Plant Tongs from Eheim.
- Spring-action with trimmer
- Use for general maintenance
- Great for moving and placing corals
- Use for feeding bottom dwelling fish
- Available in 400mm (16") or 600mm (24")
Moves things By Dave on 4/20/2017Dave would recommend this product to a friendIt moves small things on the bottom of my tank. Works as described.
Works as expected By Lisa on 2/9/2017Lisa would recommend this product to a friendPerforms well. Would have given 5 stars but for almost $20 I expected stronger construction.
Good Clamp By Jason on 1/14/2017Jason would recommend this product to a friendGreat to get same items out of the tank without having to get your hands wet. Also, works to move item around. The clamp trigger works well.
Will keep looking By RVH on 3/4/2016RVH wouldn't recommend this product to a friendDoesn't open wide enough to even easily pick up a frag plug. Difficult to operate. Ended up putting the tongs down and just reaching in with my hand. Can't see these being of much use at all in a reef tank. Disappointing product.
Hopes for more By Zach on 12/28/2015Zach wouldn't recommend this product to a friendThe product arrived broken. It was an easy fix with some BPA free epoxy. I found the range of movement to be rather narrow. I do not know if the pair I got was defective, but they did not work well for me. Going by the other reviews, I seemed to have drawn the short stick?
Good product By Tony on 6/10/2015Tony would recommend this product to a friendNice product to get wandering snails out of the tight overflows.
Great product By Guy16601 on 12/30/2014Guy16601 would recommend this product to a friendGreat item helps me from having to take my shirt off to reach in my 200 gallon aquarium as for something as siimple as turning over a turbo snail in trouble . And as always fast shipping
good product, easy to use By Dan on 5/23/2014Dan would recommend this product to a friendI have a 31" deep tank, these work well getting to small
items in the deeper areas. Easy to use.
Performs as expected By Curt on 5/20/2013Curt would recommend this product to a friendI purchased the 600mm Tongs, they provide a good amount of reach and keep my hands dry for some of the simpler task in the tank i need to perform, such as moving frags or some rubble sized rocks. The only downside I've had is that the jaws are limited to a small range and the plastic cutter/trimmer use is limited at best.
Simple and easy to use By Cody on 4/25/2013Cody would recommend this product to a friendI simply use this to do minor jobs in my aquarium and it allows me to keep my hands dry. It is affordable and gets the job done for me.
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