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Accountability. Efficiency. Service.
Investment and Fee Structure Changes Coming The Illinois State Board of investment (ISBI), is making some important Plan changes as a result of an in-depth review of the Deferred Compensation Plan's performance and structure. The main goal is to provide investment excellence and empower participants with the very best retirement planning solutions. A letter from ISBI along with a detailed brochure was mailed to all participants in the Plan. The changes will take effect beginning March 30, 2017, 3p.m. CT.
Click the following links to view the new fund fact sheets for the Vanguard Retirement Trust or the Vanguard Institutional Index 500 Trust. Fund Fact sheets for the remaining funds in the Plan may be found under the Fund Descriptions link below.
Deferral limits will remain the same for 2017. The IRS announced that pension limitations governing the 457 plans will remain the same for 2017 as the increase in the Consumer Price Index was not sufficient to meet the statutory thresholds for its adjustment. Therefore, the elective deferral (contribution) limit for employees who participate in deferred compensation will stay at $18,000. The catch-up contribution limit for employees aged 50 and over will remain at $6,000, and the Special Catch Limit is $36,000.
The State of Illinois Employees' Deferred Compensation Plan is a supplemental tax-deferred retirement plan for state employees. In 2017, employees will be allowed to defer up to $18,000 and employees over age 50 can defer $24,000. The deferrals, together with any earnings, accumulate tax-deferred until the employee terminates service, dies, or incurs unforeseeable financial hardship. Once distributions begin, the distributed monies are fully taxable as ordinary income for federal tax purposes. The funds are never taxed by the State of Illinois.
To review your account or make investment changes, you can access your account via the T. Rowe Price website.
Enroll Now and Build for Your Financial Future. Contact your Agency Liaison or the Deferred Compensation Office at the address and numbers below.
Questions and answers to address the most common inquiries about the Deferred Compensation Plan.
Examine descriptions of the funds available in the Deferred Compensation Plan with an illustration of their share price stability versus their return potential.
Access up-to-date, personal account information, including current share prices for all funds, via the T. Rowe Price website. Fund prices are shown under the 'Investments' tab. Registration required for first-time users.
Determine the investment results for each of the funds and their appropriate benchmark indexes.
Download a Deferred Compensation form or newsletter now using Acrobat Reader.
Reference Manual for Deferred Compensation Liaisons
Review a comprehensive, easy to understand guide for the Deferred Compensation Plan or the Summary Plan Description.
Whether retiring, moving to a job outside state government or leaving state service to pursue family or educational goals, you may decide how you want your Deferred Compensation account distributed. You can now contact T. Rowe Price at 888-457-5770 and request a distribution over the phone. Download a distribution booklet [PDF, 33k], W-4P Withholding Allowance Certificate, Safe Harbor Notice 2014-74, or the Direct Deposit Form [PDF, 7k]using Acrobat Reader.
CMS Deferred Compensation Office
801 S. 7th Street
P.O. Box 19208
Springfield, Illinois 62794-9208
(217) 782-7006 (Voice)
(800) 442-1300 (Toll Free)
Updated 03/09/17


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The American Museum of Natural History in Central Park, NYC. Photo Credit: worldbestspot.com
Opening Hours: Monday – Sunday 10AM – 5:45PM Closed: Thanksgiving and Christmas
Admission: Adults $20 Children $13 Seniors/ Student $16 All prices inclusive of $4 Booking Fee.
Phone: (212) 769-5100
Website: http://www.amnh.org
With an extensive collection of over 6000 prehistoric fossils, bones and specimens, the A.M.N.H is one of the largest Museums of Natural History in the world. Experience the creation of the universe within the spectacular spheric Rose Centre for Earth and Space; step into the heart of an American Rainforest; see the most unique examples of prehistoric species on Earth – you'll need a whole day to accomplish this fascinating Museum which takes visitors on a wild ride through the ages of land, sea and space exploration. Don't forget to secure your seat within the Big Bang Theater to experience the birth of Earth, and take a wander through the Butterfly Conservatory to experience the colorful living species from all over America.

subset: commoncrawl


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Jacksonville Chamber of Commerce Wall ...
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PALESTINE, May 25, 2014— Did you know that the national cost of diabetes in the U.S. well exceeds $174 billion? This figure has increased by 32 per cent since 2002. This estimate includes $116 billion in excess medical expenditures attributed to diabetes, as well as $58 billion in reduced national productivity.
People with diagnosed diabetes, on average, have medical expenditures that are approximately 2.3 times higher than the expenditures would be in the absence of diabetes. Approximately $1 in $10 health care dollars is attributed to diabetes. Indirect costs include increased factors such as absenteeism, reduced productivity, and lost productive capacity due to early mortality.
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For more information, contact Palestine Resource Center for Independent Living (PRCIL) locally at 903-729-7505 or long distance at 1-888-326-5166.
© 2017 Tomlinson-Leis Communications L.P.


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Xbox One
Looking at this popular guy's PC specs just made feel like PS4/X1 is outdated..!
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My family and I stayed here while taking part of a dance competition 20 minutes away. What a value for hotel that reminded me of a upscale hotel that I paid double for on Lake Shore Drive. QUIET!!! I know there were other guest there the lot was full. I didn't hear one toilet flush, shower, or hall walkers. Clean and fresh. Called ahead for extra pillows and towels. Didn't need to, each bed had four nice pillows and four nice sized towels.
The front desk help were friendly and helpful.
Loved that my husband made me waffles with the make you own waffles!
I will be back next time we are competing and hoping just to come for a get away.
Completely NO SMOKING, great!
- Official Description (provided by the hotel):
- At Wingate by Wyndham, we know you mean business. That's why we are dedicated to making your stay with us as comfortable and productive as possible. Each hotel is proud to offer a host of valuable amenities at no extra cost to you. Our all-inclusive pricing model gives you access to services and benefits you won't find at other mid-market hotel chains. At Wingate by Wyndham, we'll have you working hard and resting easy. From check-in to check-out, we've got it covered. Amenities include: High speed wired and wireless Internet access in all guest rooms and public spaces, Local calls and long distance access, Delicious hot breakfast with a variety of choices, Wyn-Tunes clock radio that allows you to connect your MP3 player and personalize your stay Cable TV with remote control, and in-room interactive services, Single-cup coffeemaker with Wolfgang Puck coffee, Faxes, copies and printing in the 24-hour business center, Access to a modern fitness room and relaxing whirlpool, Copy of USA Today newspaper delivered to your room Monday through Friday throughout the U.S., Convenient and easy express checkout. Comfortable, oversized guest rooms with a separate, well-lit work area Two-line speakerphone with voice mail and conference call capabilities Refreshing new bathroom amenities from well-known brands like Judith Jackson or Neutrogena Fluffier washcloths and towels Luxurious shower experience with Moen Revolution showerhead, curved shower rod and hookless shower curtain Relaxing bed experience focused on quality and comfort that includes a top of the line, comfortable new mattress and box spring as well as softer, more plush pillows and linens In-room iron, ironing board and safe Ability to earn valuable Wyndham Rewards® points, air miles or rail points Children 17 and under stay free with parents. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Hotels.com, Booking.com, Expedia, TripOnline SA and HotelQuickly so you can book your Wingate by Wyndham Tinley Park reservations with confidence. We help millions of travellers each month to find the perfect hotel for both holiday and business trips, always with the best discounts and special offers.
- Also Known As:
- Wingate By Wyndham Tinley Park Hotel Tinley Park
- Wingate Tinley Park
- Wingate Hotel Tinley Park


text: Another year, another retired list by Jen Guarino // April 30, 2014 // No Comments I used to get so sad when it was time for the retired list to come out. But now I look forward to it. Not because I want to see so many amazing products go away but because I realize... Continue reading →


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Skip to comments.One Pulitzer That Should Shake the World (Moscow Times: New York Times hid communist crimes)
Posted on 06/15/2003 8:51:20 PM PDT by DPB101
WASHINGTON -- America's most coveted journalism award is the Pulitzer Prize, and The New York Times has collected 89 of them. But now one of those Pulitzers is being challenged because the honored reporter was a fraud.
Is this about Jayson Blair, the whiz kid whose faked articles have deeply embarrassed his paper? Yes and no.
The prize in question was won in 1932 by Walter Duranty for "excellence in reporting" out of the Soviet Union. That same year, the Stalin regime sealed the borders of Ukraine, ordered the confiscation of grain, and engineered a mass famine -- one so neatly political that it stopped precisely at the Ukrainian-Russian internal border.
The Soviets called it "collectivization," the forcing of millions of people into collective farms. Ukrainians in America refer to it as the Holodomor -- roughly, the Famine-Genocide -- and they consciously use a capital "H" in imitation of the Holocaust.
The Holocaust -- the killing of about 6 million Jews, along with some 3 million Soviet POWs and thousands of Gypsies -- is woven into the textbooks, the consciousness and the monuments of nations everywhere.
And the Holodomor? It claimed some 7 million innocents. At its height, while the Soviets exported thousands of tons of grain to the West, Ukrainians were dying at a rate of 25,000 per day. Yet no one has heard of it. Every November, the U.S. president sends a short letter to Ukrainians marking the tragedy. Other than that, it passes virtually unmentioned.
To understand how the Holodomor slipped down the memory hole, one has to look back to the 1930s. The Great Depression was on, and in the West communism was admired or feared. That, plus the Soviet practice of deporting critics, soon filled the Moscow foreign press corps with apologists for Stalin.
Duranty was not alone. (Another apologist, Eugene Lyons of UPI, repented and wrote one of my favorite books, "Assignment in Utopia." Check out chapter XV, "The Press Corps Conceals a Famine," at www.colley.co.uk/garethjones/soviet_articles/assignment_in_utopia.htm)
But Duranty was unusually cynical. He would talk about millions of famine deaths, and then add, "But they're only Russians," and, "you can't make an omelette without breaking eggs." And incredibly, he won the Pulitzer for reporting in 1931 on Stalin's Five-Year Plans.
This year marks the 70th anniversary of the Holodomor, and in January the Ukrainian Congress Committee of America launched a campaign to have Duranty's Pulitzer rescinded. The Pulitzer board is formally studying that. But in the past, the board has split hairs, arguing that Duranty's Pulitzer was for reporting that predated the famine and had nothing to do with it, while The New York Times has taken the position that its own pages have since denounced and debunked Duranty's work, and his Pulitzer is displayed with an asterisk to that effect at Times' headquarters. And that's apparently good enough.
So, a cub reporter publishes a string of articles that plagiarize or embellish upon some pretty minor realities -- and this provokes a monster mea culpa on the front page detailing the paper's sins, followed by the resignations of its editors. Meanwhile, another reporter is known to have been a serial liar, someone who actively worked over many years to cover up the equivalent of the Holocaust -- and The New York Times admits as much, yet feels OK holding on to his Pulitzer.
Doesn't that tarnish the other 88?
Matt Bivens, a former editor of The Moscow Times, writes the Daily Outrage for The Nation magazine. [www.thenation.com]
Gareth Jones is the Welsh reporter who first reported on the Holodomor. His great nephew posted on Free Republic earlier today here:
The Jones family is understandably delighted that Gareth is finally receiving the recognition he deserves (Gareth was only 29 when he killed by bandits in Munchukuo while investigation Japanese expansion into Northern China).
A great archive of articles by Gareth Jones, Malcolm Muggeridge and the notorious 1933 "omelet" Moscow dispatch by Walter Duranty is at the Jones website here:
And if history and the recording of truth relied upon the American "mainstream newsmedia", a helluva lot more than that would "slip" down the memory hole!
"Doesn't that tarnish the other 88?"
Yes, of course, but that's far from the only thing.
And so much for that "newspaper of record" schtik.
Truth itself is the only "newspaper of record"!
Yes, and they're still at it.
"Indochina without Americans: for most, a better life."
From Kolyma - Land of Gold and Death, by Stanislav Kovalski:
"One dose of Stalin's "truth" was presented to the American Vice-President, Henry Wallace, when in 1944 he visited Kolyma. After the visit he left the country with the absolute conviction that "no such camps existed," in total agreement with the British philosopher, Bertrand Russell. Whatever was shown to the man who held one of the highest offices in the USA he accepted it as the truth and presented it in his book "Soviet Asia Mission." From this book the American reader learned that the gold miners of Kolyma are "big husky young men who came out to the Far East from European Russia," and who were "pioneers of machine age, builders of cities." He was greatly impressed with Kolyma's director Nikishov and his wife Gridassova, with Magadan's cultural life and with the shops full of Russian goods. The truth was that during his three-day stay the chiefs of Kolyma did their best to conceal the factual reality. The wooden watchtowers were pulled down, the prisoners were not allowed to leave their barracks and not even the least aspect of prison life was exposed to the American visitor. He was taken to the only farm in the region, 23 kilometers from Magadan, where well dressed and well fed girls, (police women disguised as swineherds), gave a false impression of the agricultural endeavor in that part of the country. He was also flown to the North, to the mine Berelakh, where he found the state mining to be an impressive enterprise."
"The miners, according to him, were healthy and well-clad men, and more productive than their counterparts in Alaska's Fairbanks. Being served with delicious fresh fish from Kolyma River he offered his compliments to the "presiding chef of the mining camp." The deception was total and successful. The outside world got the firsthand knowledge about Kolyma from the man who deserved his trust. Who would disbelieve or dispute information from the Vice-President of the United States of America, a force for truth and justice? "
Forget who it was--either Harry Dexter White or Lauchlin Currie (both close to FDR and both Soviet agents) went with Wallace on his tour of the Soviet gulag.
Joe McCarthy was right!!
"Indochina without Americans: for most, a better life."
"These people don't value human life like we do."
"Kill a gook for God!"
"The growing hysteria of the administration's posture on Cambodia, seems to me to reflect a determined refusal to consider what the fall of the existing government in Phnom Penh would actually mean.... We should be able to see that the kind of government which would succeed Lon Nol's forces would most likely be a government ... run by some of the best-educated, most able intellectuals in Cambodia."---Senator George McGovern
"The greatest gift our country can give to the Cambodian people is peace, not guns. And the best way to accomplish that goal is by ending military aid now."--Chris Dodd (D-CT)
I give Senator McGovern credit however. When he learned of what the Khymer Rouge were doing, he wanted to send American troops back in. That took some guts even to suggest back then.
Disclaimer: Opinions posted on Free Republic are those of the individual posters and do not necessarily represent the opinion of Free Republic or its management. All materials posted herein are protected by copyright law and the exemption for fair use of copyrighted works.


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This product is no longer available.
You can try these similar gifts instead.
Shiny Sterling Silver Stacking Rings
Sterling silver has never looked so glamorous! This set of three stacking rings is hammered to the point of illumination, lending each ring a glass-like effect that adds beauty to any ensemble.


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What we do
ideas that help create smart cities and make them more liveable and sustainable. We contribute to expand the work of eco producers based in Europe and all over the world. Working locally, sharing working spaces, dealing with new technology that enhances them to produce their goods and selling them,and making from that their living. We consider ourselves an awareness network. As a digital platform for collaboration, our contents originate from our global contributors. Our main goal is allow the user to also produce content , with an open Access Ecosystem approach that has the potential to empower citizens and increase participation.
Who's involved
Organisations- Smart Volta
This project was last updated 1 year ago


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Vote YES for SJS - CSU Initiative
Expand Messages
- San Jose State-CSU : Vote 'Yes'VOTE "YES" March 20-21 for the SJS/CSU Identity. Online voting via MySJSU is allowed! The Initiative would:
Alumnus Endorses SJS-CSU Identity InitiativeAfter seeing our proposed San Jose State - California State University logo (above), alumnus James Rowen has endorsed the Alternate Identity Initiative on his blog, saying its a step in the right direction. See http://missioncitylantern.blogspot.com/2007/03/vote-yes-on-name-change.htmlIf you have a website, please endorse the Initiative and let us know about it.Join the CSU Students of San JoseThe Initiative is sponsored by the 300-member CSU Students of San Jose. Join us:
- Preserve the "San Jose State" name as the school's primary identity
- Add a secondary identity that includes the words "California State University", such as "CSU San Jose", "CSU Silicon Valley" or "CSU (1862)"
- Restore the rights of San Jose State students to choose to identify with and market themselves under the CSU name
- Restore San Jose State's prestigious and valuable identity as the original and oldest CSU campus
Vote in Gostate.org PollPlease also vote in the www.gostate.org straw poll on the best city-state identity for San Jose State. Vote here: www.gostate.org
- http://groups.yahoo.com/group/CSUstudentsSanJose
- http://groups.myspace.com/CSUStudentsofSanJoseState
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CHRIS Basham and Bob Harris both made welcome returns to action yesterday in a behind-closed doors game at Sunderland.
Basham hasn't played since breaking his cheekbone against Leicester City last month.
The 24-year-old has made a remarkable recovery, only two-and-a-half weeks after having an operation to correct the damage.
He played 90 minutes in a strong looking second string at the Premier League side's training ground.
Blackpool lost 2-1, with Harris scoring Pool's goal.
Harris has not featured since breaking his toe in the 2-1 home defeat to Cardiff in January.
The full-back was on the scoresheet in a Pool line up at Sunderland which included Elliot Grandin, Alberto Noguera, Nathan Eccleston and James Caton.
The previous evening Basham turned his hand at something totally different, cooking dinner for fans!
He was joined by Harris for the first sign and dine evening held by Rowley's Restaurant at Bloomfield Road.
Supporters enjoyed a three course meal cooked by the pair, before meeting them for autographs and pictures.


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Use the form on the right to contact us.
For returns and exchanges please view our returns and exchange page HERE
For wholesale inquiries please write to firstname.lastname@example.com
To Write an encouraging message please go HERE
123 Street Avenue, City Town, 99999
(123) 555-6789
You can set your address, phone number, email and site description in the settings tab.Link to read me page with more information.
Designed for the girl who roams. The Mahlia Maxi won't hold you back, in fact it will encourage you to go further. Its flowy design keeps you cool while you explore. It's center half-moon hem makes this dress work for all sizes, shapes and heights. Features:-V neckline-Two Adjustable straps on each side-Half Moon Hem-Side SlitsOur Heart: All YIREH products are ethically produced in small batch collections. Everything is completely handmade, which gives us the opportunity to employ and work with multiple artisans, and incorporate traditional craftsmanship into our collections. We donate excess fabric to charities in Bali to minimize our textile waste. 10% of our profits are also donated to International Justice Mission, an organization geared towards freeing, and seeking justice for victims of Human Trafficking.


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Skydiving Classifieds
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text:
DUBLIN, May 5, 2016 /PRNewswire/ --
Research and Markets has announced the addition of the "Global Insomnia Market and Competitive Landscape Highlights - 2016" report to their offering.
Global Insomnia Market and Competitive Landscape Highlights 2016, provides comprehensive insights into Insomnia pipeline products, Insomnia epidemiology, Insomnia market valuations and forecast, Insomnia products and competitive landscape.
This report is classified into eleven sections - Insomnia overview including etiology, pathophysiology, and diagnosis, Insomnia treatment options, Insomnia pipeline products, Insomnia market analysis globally and by each country. The market analysis comprises of Insomnia epidemiology, key products marketed for Insomnia, market valuations and forecast, products sales and market shares.
Research Scope:
- Insomnia Pipeline: Find out the products in clinical trials for the treatment of Insomnia by development phase 3, phase 2, and phase 1, by pharmacological class and companies developing the products- Insomnia Epidemiology: Find out the number of patients diagnosed (prevalence) with Insomnia- Insomnia Products: Identify key products marketed and prescribed for Insomnia, including trade name, molecule name, and company- Insomnia Product Sales: Find out the sales revenues of Insomnia products, both branded and generics- Insomnia Market Valuations: Find out the market size for Insomnia products in 2015 for the markets: Find out how the market advanced from 2010 and forecast to 2020- Insomnia Products Market Share: Find out the market shares for key Insomnia products
Key Topics Covered:
1) Disease Overview
2) Insomnia Treatments
3) Insomnia Pipeline
4) Global Insomnia Market Insights
5) US Insomnia Market Insights
6) Germany Insomnia Market Insights
7) France Insomnia Market Insights
8) Italy Insomnia Market Insights
9) Spain Insomnia Market Insights
10) UK Insomnia Market Insights
11) Japan Insomnia Market Insights
12) Methodology
For more information visit http://www.researchandmarkets.com/research/9b698k/global_insomnia
Media Contact:
Research and Markets
Laura Wood, Senior Manager
For E.S.T Office Hours Call +1-917-300-0470
For U.S./CAN Toll Free Call +1-800-526-8630
For GMT Office Hours Call +353-1-416-8900
U.S. Fax: 646-607-1907
Fax (outside U.S.): +353-1-481-1716
To view the original version on PR Newswire, visit:http://www.prnewswire.com/news-releases/global-insomnia-market-and-competitive-landscape-highlights-2016---forecasts-to-2020---research-and-markets-300263433.html
SOURCE Research and Markets


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STARmeterSEE RANK
Up 137,277 this week
Emily Cranz
Emily Cranz was born on September 21, 1942 in Tucson, Arizona, USA as Emma Cranz Cantillano. She is an actress, known for Báñame mi amor (1968), Con licencia para matar (1969) and Pasión oculta (1967). See full bio »
Born:in Tucson, Arizona, USA


text:
I'm using an animation to change the alpha channel of a material to get a fade effect.
When you add the color property of the material in the animation it adds all 4 rgba channels. You can then remove rgb and only have the alpha channel to animate. When this animation runs it will set the rgb values all to 0, turning the material black.
You can just not remove rgb to fix this issue but then there is another problem. It will set rgb to the initial values of the material color. If you change the material color afterwards, the animation will be unchanged and still contain that initial color. So I would have to update all those animations by hand.
I'm using the standard shader in transparent rending mode and Unity 5.5.0f3.
Edit: Made a test project to demonstrate this.
Up to 2 attachments (including images) can be used with a maximum of 524.3 kB each and 1.0 MB total.
The best place to ask and answer questions about development with Unity.
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Answers and Comments
5 People are following this question.
send csv colour values to multiple meshes
Can I make animations snap to a frame?
Adjust tint color of particles/additive?
How can I control all the meshes' materials in an object?
Switch between random colors


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Roberts County District Attorney 414 3rd Avenue East Sisseton SD 57262 6.8 miles from Goodwill 605-698-7071
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Marshall County District Attorney PO Box 107 Britton SD 57430 37.1 miles from Goodwill 605-448-2775
Codington County District Attorney 14 1st Avenue Southeast Watertown SD 57201 46.2 miles from Goodwill 605-882-6276
Sargent County State Attorney PO Box 125 Forman ND 58032 46.5 miles from Goodwill 701-724-3888
A district attorney prosecutes cases on behalf of the public. District attorneys manage the prosecutor's office and play a central role in investigating charges, deciding whether to charge someone with a crime, offering plea bargains, and litigating appeals. They also advise parole boards on inmate requests for early release.
District attorneys maintain records on current and former cases. Many of these records are available online and open to the public. This site can help you access the records you need from a district attorney.


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Download Bali Travel Guide apk 1.1.4 free for Android smartphone
Posted by Application Nexus in Travel & Local | Nov. 16, 2015 | 21 Comments
Apk file size: 30.0 MB
With detailed information of around 20 museums , 10 parks and 100 featured attractions along with 1000s of other Points of Interest carefully integrated with every single travel activity, Bali travel is power packed with utilities and information. From Facebook Check-Ins to local event information, offline map to intuitive Augmented Reality experience, we have brought most of the travel activities and application needs to one single app. Save precious travel time rather than switching between several apps and utilities!
• Practical travel guide with detailed information like attractions, getting around for each district of Bali City.
• Travel utilities like hotel booking, currency converter, weather forecast, world clocks, reminder, alarm and many more available within the app.
• Intuitive, easy-to-use one tap pane interface with tight social network integration for Check-Ins, photo sharing and places review.
• Just one app for all your devices with dedicated UI and data sync capabilities.
• Plan your itinerary and port your activity data between different devices and social network using our pangeaguides.com website.
• Community sharing experience for your photos, reviews and travel memories with your friends and other fellow travelers.
Premium features of Bali Travel Guide bring exclusive travel utilities that reduce travel planning hassles and make the experience fun and memorable. Premium features consists of -
• A next generation modern marvel Augmented Reality experience that brings surrounded by wonders feeling.
• Search for cafes, restaurants, theatres and even hospitals with carefully organized location data. Find more than 1000 Points of Interest around you and get directions to places of your interest.
• Extremely detailed Bali offline map for no connectivity areas or internet availability (Connection required on first time use).
• Comprehensive set of utilities and tools that will help you plan your trip. Organize checklist, send postcards, bringing a collection of useful travel apps within a single app.
• Discover people, places and culture with information about upcoming local events.
Pangea Guides are Designed and developed by the travelers, for the travelers that works in all parallel universe!
Whats new
• Introducing Social Sharing. Now share places you are visiting using Facebook and Twitter.
• Improved Offline capability from the moment it is installed.
• Tightly integrated with Facebook for places Check-Ins.
• Improved Photo Albums and sharing capabilities.
• One touch localization for currency weather and measurement units.
• Emergency phone numbers data updated.
• Improved social features for group travel.
Application Nexus part of our Travel & Local and have average installs from 10000 to 50000. Last Update Nov. 16, 2015. Google play rating is 78.2692. Current verison is 1.1.4. Actual size 30.0 MB.
Similar applications
BALI GUIDE JP Tour Travel info28 Comments
21 Comments
Michael El
BUYBUYBUY Why releasing a free version with absolutely NO features, if you need to buy it anyway to try it out?? Bit silly, isn't it? I wont buy before I am able to check functionality.
Hilman Hillzx
Dislike the design The ui is not easy to use
Isabel Newton
Very informative.. Multiple utilities in the single app. Superb data collection for museums, attractions, park, restaurants etc. Love the offline map feature.. You have pay to get access for some features but they offer lots of stuff in no cost..
Meg W
Nice tour guide!!! It's more of a local tour guide in your pocket. I'm out of town at least once a month, for business mostly. This app relieves me of my travel hassles...
Анна Орлова
Very useful Sufficient information, up-to-date prices, accurate directions. Thanks a lot!
Simon Derbaix
Don't waste your time! The app will not full function without in app purchases (offline map,...) and the currency converter does not work. Mind that most reviews before me are fake.
Laszlo Takacs
cool Great app, Recommend it! 5 stars
Dragos Cristi
Very useful I was searching for something like this for a long time
Prasanna Venkatesh
Hey Bali is the place i wanted to visit Cool app with most of the places covered good travel guide.
Jaimin Patel
bluelex awesome!! feels like using windows 8.1 features..cooooooolll..!! worth downloading..<3 <3
Prasaad Joshi
Awesome Really beautiful application guys. I'm expecting something very much close to me i.e. Coastal Regions around India. I've seen your application about Nashik wanna suggest something about Pune n Konkan...
anand kumar Kurapati
Great Too useful travel guide and very nice UI
Ryan Shaw
Dont bother Unless you're prepared to pay $5 for something you can't even preview don't bother. The concept appears good however you can't preview the content properly until you've paid. My experience with these apps is they only provide a small amount of information anyway.
mob jax
Very Nice App Excellent App for knowing everything in detail.
Pranjal Jeswani
Great App Amazing work by developers Keep it up
Lee Broadstock
Nice app Very informative. And very helpful. Nice work
nabeel zafar
informative outstanding informative app. I will must recommend this app to my friends
Mia Wilson
:) It's great!
Jenny Barton
awesome ,, very useful app..
Neo Anderson
very useful & nicely implemented :)
Nice app very useful app


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Tuesday, August 2nd, 2011 at
11:45 am , filed under
Celebrities by
Many men will probably not able to get out of their heads when the thought of Nichole Scherzinger is in the shower. An image of Nicole has been released with a very hot look ahead of the release of her new single wet and appears to show her in the shower.
Nicole Scherzinger is clutching a net round her body and she looked sensational in her taupe two pieces and she stared wistfully off into the distance. Unfortunately, for her male admirers, Nicole was not actually taking a shower at all but has teased her fans by standing behind a wall of water. However, the still was taken from a video for her new single which is due in release on 28th August.
Scherzinger has managed to film the video during a rare day off from her busy X-Factor schedule that has been now on hold until the show starts next month. Initially, Nicole has announced at one of the two co-hosts of the show alongside Britain's Steve Jones but has ended up replacing Cheryl Cole on the judging panel alongside Paula Abdul Cowell and L.A. Reid.
Last night, her boyfriend Lewis Hamilton has helped to celebrate McLaren teammate Jenson Button's Hungary Grand Prix in Nicole has went dancing in Los Angeles. She has been photographed with a male friend as she left a nightclub.
Tags: Nicole Scherzinger, Nicole Scherzinger shower photo, Nicole Scherzinger wet photo


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Thomas Jefferson tight end/defensive end Brock DeCicco (6' 4" 210) treated his visit to the U.S. Army All-American combine like another day at the office.
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Tyler Farr
Tyler Farr's Jason Aldean Impersonation Includes Moves and Jabs [Watch]
At Taste of Country Festival in early June, Tyler Farr shared the best impersonation he's got, and it happened to be his friend and fellow country superstar Jason Aldean.
Watch a Trailer for Tyler Farr's Insanely Redneck New Reality Show
In his upcoming reality show, A Little Too Farr, Tyler Farr reveals just how much shenanigans a backroad country star can get into.
Tyler Farr's 'A Little Too Farr' Reality Show Launching June 2
Tyler Farr's new reality series, 'A Little Too Farr,' is set to launch online on June 2.
Lady Antebellum, Tyler Farr Set for 2017 Super Bowl Pre-Game Party
Lady Antebellum and Tyler Farr are both getting in on the action at Super Bowl LI. The country stars will perform at an exclusive pre-game party prior to the Super Bowl on Feb. 5 at NRG Stadium in Houston.
Lady Antebellum, Tyler Farr to Perform at 2017 Super Bowl Pre-Game Party
Lady Antebellum and Tyler Farr will perform at a pre-game party for the 2017 Super Bowl.
Country Stars Who Got Engaged or Married in 2016
A number of country stars have celebrated love this year with an engagement or a wedding.
Tyler Farr Ready to Go 'Too Farr' in New Reality TV Show
Tyler Farr is set to become a reality television star. The singer says a new show called A Little Too Farr will air in 2017 on a new television network.
Brad Paisley Serenades Tyler Farr and New Wife During First Dance [Watch]
Tyler Farr's wedding day was no doubt a very special occasion, but Brad Paisley made the day even more memorable.
Tyler Farr Marries Hannah Freeman
Congratulations to Tyler Farr and his fiancee Hannah Freeman -- they're now husband and wife!
Jason Aldean Is Producing Tyler Farr's Next Album
Tyler Farr has revealed that the producer for his third studio album is none other than fellow country star Jason Aldean.
What Did Your Favorite Country Stars Look Like as Children?
Have you ever wondered what your favorite country singer looked like when he or she was pint-sized?


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"The beautiful spring came; and when nature resumes her loveliness, the human soul is apt to revive also."
-Harriet Ann Jacobs
There are only a few more weeks of spring. With the joys of summer approaching, it is easy to skip over these last weeks and jump to the good times ahead. But there is still time to get done all of the items on the Spring to-do list. Tackle the spring-cleaning projects. Plant flowers. Change out the front door wreath. Eat plenty of fresh strawberries.
{ Photo 1 from Sweet Onida's Facebook last weekend; Photo 2 by Pamela Claytor at Sweet Ila Mae's }
Coming up next week on the blog, you will have a chance to win a colorful Corkcicle tumbler and learn a recipe for peanut butter cookie dough cheesecake. Don't forget, this weekend is Til The Cows Come Home. You can also visit The Downtown Green Market Saturday morning. Happy Mothers Day!


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Ireland is well known for a lot of things. Guinness (The Irish consume more beer per head than any other country in the world); leprechauns; passion; sport; fighting and a lot more. Those are ordinarily what people think of when they think or Ireland right? Well every country and culture has hidden gems as well, and that is what I'm hoping the review today will turn out to be- it's Cadbury Ireland's Golden Crisp bar. Without even a drop of Guinness.
I've seen the Golden Crisp before from Cadbury Ireland as I've got a mother who grew up there and adores their Tiffin bar which is from the same 54g range. I can even remember trying their Caramello bar a long, long time ago whilst in Ireland…although that was far before my reviewing started so I couldn't tell you what it was like. It did have caramel in it though- you can have that info for free!
The Golden Crisp is 'milk chocolate with golden honeycombed granules'. Or in other words, it is a bar of their Dairy Milk chocolate with crumbled up bits of Crunchie in it by the sounds of it.
I can now add Cadbury Ireland to the growing list of varied Cadbury companies that I've tried- UK, U.S, Canada, Australia, South Africa and (most bizarrely) Egypt. Currently it is the Egyptian chocolate, found on their SMS bar just before Christmas, which leads the way from our very own British stuff. This Cadbury Ireland product was sent to me by CyberCandy– so thanks for that guys!
The shape of the Golden Crisp is probably the most notable visual thing about it. It is a 54g bar and a single serving size (50-60g is the norm for individual chocolate bars) but rather than the long and thin rectangular shape, Cadbury Ireland have gone for a shorter and fatter approach with 8 individual squares to it. And it's a bit of an optical illusion- it looks quite small in your hand initially, more like a child sized bar. Yet when you get into it, the 8 pieces are plenty and just as filling as a normal shaped bar.
I actually like the change to be honest- as a non-Irish customer, this has got some novelty value and the change up in shape makes the Golden Crisp that little more interesting. The wrapper itself isn't all that inspiring…it is aesthetically as you'd expect from Cadbury. ("What have we got boys?" "Urgh, a Dairy Milk Golden Crisp, boss." "Okey dokey, throw in some Cadbury's purple, throw in some gold and we're sorted.") It is tidy without being remarkable.
Taking photos (I'm like a fashonista/photographer trying to make my subject look as pretty as possible!) I broke into the pieces for the first time and was astonished at how crisp the honeycomb chunks were. Well, 'chunks' is the wrong word, they're sort of like Rice Krispies cereal in size but ridiculously crunchy. You really have to work to crack open the firm chocolate, but when you do, you can actually feel the bar breaking around the honeycomb nibs- they're so strong that they don't crack in the half, the chocolate just breaks around them!
I'd expected it to smell like a Crunchie bar. My reasoning? Well, Cadbury's honeycomb plus Cadbury's milk chocolate = the same smell. I was wrong. This is much more chocolate heavy; it's milk chocolate with a little honeycomb rather than vice versa like a Crunchie is. Cadbury Ireland's Dairy Milk is totally different to our UK version- the Golden Crisp is covered by more chocolatey and less milky stuff.
The chocolate here is mouth-watering and lusciously creamy. I'd describe it as thicker than any other Cadbury's chocolate I've tried before- when it melts, it is almost like having double cream in your mouth rather than a glass of milk. And I absolutely love it- the melt is thick but never too gloopy and the sweetness is toned down from British Dairy Milk with a tad more richness to accompany the honeycomb. Not beating around the bush, this is my favourite Dairy Milk recipe I've tried from around the world, usurping Cadbury Egypt's stuff which is knocked down into the number two spot.
The honeycomb though, is a fail. As I wrote earlier, there are plenty of golden honeycomb nibs and they're all cool and crispy and fun to chew on. But they don't taste of anything. Cadbury's Crunchie bar has a cracking toffee flavour to the honeycomb, but these little bits are just about tasteless. Not bad, not offensive…but just bland and dull.
So, my final thoughts of Cadbury 'Oiland's' (say it aloud, you'll get it!) Golden Crisp? Well, this bar was all about the Dairy Milk chocolate which was cracking. Shame about the rather weak honeycomb aspect, but the milk chocolate was tasty enough to cover the shortfalls of the honeycomb. It is ridiculously heavy and creamy, and it might even be too much for some people…but I laaaaaav it!
Would I eat it again? Absolutely yes. But not before I try some of the other bars from the same Cadbury Ireland 54g range in the form of their Tiffin and Caramello bars…if you're reading this Tiffin and Caramello…I'm coming for you boys. Don't even try and run- you're mine.
Rating- 8.0/10. (Chocolate would be higher…but it's supposed to be a honeycomb bar!)


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An exclusive today and something a little different from OhYeah!?. Not an edit but a really accomplished cover of the Evelyn Champagne King classic 'Love Come Down'. We loved this one from the off, the original is a firm favourite but this version from duo OhYeah!? is laden with instrumental work (the real kind) and a fantastic vocal performance from vocalist Fleur. It's really refreshing to hear a cover of a track that doesn't just slice up the original and instead opts to rebuild in an interesting way in a studio. I'm pretty sure this would put a smile on Evelyn King's face.
If you HAVE twitter or facebook then here's a free download


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These lyrics that promise
Unconditional Love,
Merciful Healing,
Eternal Bliss.
They latch onto my soul –
Stirring it until I am swept away
by waves of curiosity;
Spinning it until I am lost in
currents seeking truth.
Eventually, I am relinquished by the cold splash of realism.
It is as if a storm never even existed.


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Charcoal and acrylic on pages of the Gospels | 140x120cm | SOLD
Excited to be starting a new role as International Programs Manager with UNICEF Australia this week. Here's to a n…… https://t.co/CP2WKklGzU
Tonight at 9pm I'll be talking about art and portrait painting with Hope 103.2's The Big Picture. Here is a second…… https://t.co/CK9HIEQUnR
About a year ago, Nelson woke up and he was blind. Just like that. The doctors couldn't cure him. His wife had to …… https://t.co/NrrL3jHAfp


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The space
"Caciaia" is a small apartment of Podere Baccano (Montozzi - Arezzo) (2+1 persons). Inside Caciaia, you will find the kitchen with fireplace, table, television, bedroom with double bed and easy-chair (second bed in case of third person), bathroom with shower.
2+1 bed, 1 bathroom, 1 kitchen with fireplace.
Holyday in Podere Baccano offers you the chance to stay in beautiful apartment if you want to spend a couple of weeks in the Tuscan countryside. "Caciaia" is cosy and warm, which is typical of Tuscan country homes.
The apartment tucked away in silence and that is why it can offer peace and quiet as well as maximum privacy.
"Caciaia" apartment is furnished according to the typical country style, with a kitchen and fireplace, private space with table and chairs, where you can have lunch, dinner or simply look out on to the landscape.
A small swimming pool (10x5 m.) inide 500 olive trees, and 20 hectares of woodland, where it's possible to meet some native animals, like roe deer, fallow deer, wild boar...
Guest access
Guests have access to the patio in front of the apartment and around the garden of the property, the use of the swimming pool in the olive grove (shared with second apartment) and a whole forest of 20 hectares in which you can walk, look for mushrooms and observe closely the animals that populate it (foxes, wild boars, cats, deer, wild sheep, deer, wolves).
Interaction with guests
During your staying you can count on our daily presence, and if we were not present, you will have 24 hours 24 our phone numbers. We will be glad to help you to solve any kind of problem.
Other things to note
Piero, my dad, has an organic garden witch in summer offers delicious vegetables: tomatoes, zucchini, eggplant/aubergine, carrots, potatoes, celery, onions, garlic. Fruit trees such as plums, apples, pears. The two olive groves protect 500 olive trees that in November give the olives from which we get the best oil in the world. In the garden in front of the apartment you will find herbs you can use in the kitchen: basil, sage, fennel, nipitella, rosemary and mint. Ripe figs can capture directly from 12 fig trees spread throughout the grounds.
Pets are allowed. It could be better Smocked outside.
5 Reviews
This host has 1 review for other properties.View other reviews


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Several studies have suggested that activation of p70 ribosomal S6 kinase (p70 S6 kinase) by insulin may be mediated by the phosphatidylinositol 3-kinase (PI 3-kinase)-Akt pathway. However, by temporal analysis of the activation of each kinase in L6 muscle cells, we report that the activation of the two serine/threonine kinases (Akt and p70 S6 kinase) can be dissociated. Insulin stimulated p70 S6 kinase in intact cells in two phases. The first phase (5 min) of stimulation was fully inhibited by wortmannin (IC50 = 20 nM) and LY-294002 (full inhibition at 5 μM). After this early inhibition, p70 S6 kinase was gradually stimulated by insulin in the presence of 100 nM wortmannin. After 30 min, the stimulation was 65% of the maximum attained in the absence of wortmannin. The IC50 of wortmannin for inhibition of this second phase was ∼150 nM. In contrast, activation of Akt1 by insulin was completely inhibited by 100 nM wortmannin at all time points investigated. Inhibition of mitogen-activated protein kinase/extracellular signal-regulated protein kinase kinase with PD-098059 (10 μM) or treatment with the protein kinase C inhibitor bisindolylmaleimide (10 μM) had no effect on the late phase of insulin stimulation of p70 S6 kinase. We have previously shown that GLUT-1 protein synthesis in these cells is stimulated by insulin via the mTOR-p70 S6 kinase pathway, based on its sensitivity to rapamycin. We therefore investigated whether the signals leading to GLUT-1 synthesis correlated with the early or late phase of stimulation of p70 S6 kinase. GLUT-1 synthesis was not inhibited by wortmannin (100 nM). In summary, insulin activates p70 ribosomal S6 kinase in L6 muscle cells by two mechanisms, one dependent on and one independent of the activation of PI 3-kinase. In addition, activation of Akt1 is fully inhibited by wortmannin, suggesting that Akt1 does not participate in the late activation of p70 S6 kinase. Wortmannin-sensitive PI 3-kinases and Akt1 are not required for insulin stimulation of GLUT-1 protein biosynthesis.
- glucose transporters
- mTOR
- wortmannin
- rapamycin
the ribosomal p70 S6 kinase is a serine/threonine kinase that is activated in response to many growth factors by hierarchical phosphorylation of multiple serine and threonine residues in discrete functional domains (34). In vivo, the kinase controls protein synthesis by increasing the rate of translation of a family of mRNAs that encode essential components of the protein synthetic machinery (32). In addition, we have recently reported that the macrolide immunosuppressant rapamycin, which prevents the activation of p70 S6 kinase, blocks the insulin-induced upregulation of glucose transporter 1 (GLUT-1) expression (39, 40). The molecular mechanisms underlying the activation of p70 S6 kinase by insulin and other growth factors are poorly understood, and some of the intermediate participants that link growth factor receptors to the phosphorylation and activation of this serine/threonine kinase remain elusive.
Phosphatidylinositol 3-kinase (PI 3-kinase) is a lipid kinase that phosphorylates inositol lipids at the D-3 position (7), and several lines of evidence support the view that it is one of the primary signaling molecules linking growth factor receptors to the activation of p70 S6 kinase (8, 10). For example, platelet-derived growth factor (PDGF) failed to stimulate p70 S6 kinase in Chinese hamster ovary (CHO) cells overexpressing a mutant PDGF receptor lacking the binding sites for the p85 regulatory subunit of PI 3-kinase (Y740 and Y751) (10). Moreover, two structurally different inhibitors of PI 3-kinase, wortmannin and LY-294002, blocked the activation of p70 S6 kinase by insulin in 3T3-L1 adipocytes (8) and by PDGF in CHO cells overexpressing wild-type PDGF receptors (10). On the other hand, mutation of Y740 in the PDGF receptor completely abolished activation of PI 3-kinase by PDGF but did not prevent the activation of p70 S6 kinase (27). In addition, insulin can activate p70 S6 kinase in CHO cells and Rat1 fibroblasts, both overexpressing a dominant negative p85α subunit of PI 3-kinase, which lacks the binding site for the p110 catalytic subunit (17). Surprisingly, wortmannin (50 nM) completely inhibited insulin-induced activation of p70 S6 kinase in both cell types expressing dominant negative p85α (17). These results suggest that a PI 3-kinase-independent but wortmannin-sensitive pathway exists for the activation of p70 S6 kinase by insulin.
The molecular mechanism underlying the activation of p70 S6 kinase is complex, but one current model suggests that a PI 3-kinase-dependent kinase phosphorylates Thr389, one of the most critical residues for p70 S6 kinase activation (12). Akt is a serine/threonine kinase that has been demonstrated to be a downstream target of PI 3-kinase in cells stimulated with insulin (23), PDGF, epidermal growth factor (EGF), or basic fibroblast growth factor (4,15), since activation of Akt by these growth factors is blocked by wortmannin and LY-294002. Activation of Akt was also lost in cells overexpressing a dominant negative p85α subunit of PI 3-kinase (4). In addition, Akt could not be activated by PDGF in cells overexpressing the PDGF-receptor mutants described above (4, 15). On the basis of the characteristics of the activation of Akt and the observation that overexpression of a gag fusion of Akt constitutively activates p70 S6 kinase (4), it has been speculated that Akt could be the protein kinase that mediates the PI 3-kinase-dependent phosphorylation of Thr389 of p70 S6 kinase. On the other hand, Akt cannot phosphorylate p70 S6 kinase in vitro (33), and a kinase-deficient mutant of Akt believed to have dominant negative action was unable to block p70 S6 kinase activation in vivo (22).
Given the controversy mentioned above, we have investigated the time course of p70 S6 kinase activation vis-à-vis its sensitivity to inhibition of PI 3-kinase. Surprisingly, two mechanisms of activation were uncovered, one dependent on and one independent of PI 3-kinase, based on sensitivity to wortmannin and LY-294002. We further demonstrate that, in contrast, activation of Akt1 is fully dependent on PI 3-kinase and can therefore be dissociated from the activation of p70 S6 kinase at longer times.
α-MEM, FBS, and other tissue culture reagents were purchased from GIBCO BRL (Burlington, ON, Canada). Human insulin (Humulin) was obtained from Eli Lilly Canada (Toronto, ON, Canada). Protein A-Sepharose was from Pharmacia (Uppsala, Sweden). Polyclonal anti-GLUT-1 glucose transporter antiserum was from East Acres Laboratories (South Bridge, MA). The monoclonal antibody 6H to the α1-subunit of the Na+-K+-ATPase was a kind gift from Dr. M. Caplan (Dept. of Cellular and Molecular Physiology, Yale University). Polyclonal anti-p70 S6 kinase, anti-Akt1, and anti-IRS-1 antibodies, p70 S6 kinase peptide substrate, and protein kinases A (PKA) and C (PKC) inhibitor peptides were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Akt1 substrate peptide, Crosstide, was purchased from Upstate Biotechnology (Lake Placid, NY). [γ-32P]ATP (6,000 Ci/mmol) and enhanced chemiluminescence reagents were purchased from Amersham (Oakville, ON, Canada). Purifiedl-α-phosphatidylinositol (PI) was purchased from Avanti Polar Lipids (Alabaster, AL). Oxalate-treated TLC silica gel H plates (250 μm) were from Analtech (Newark, DE). Wortmannin was from Sigma (St. Louis, MO). PD-098059 was a kind gift from Dr. Alan Saltiel (Parke-Davis, Ann Arbor, MI). LY-294002 and okadaic acid were from Biomol (Plymouth Meeting, PA). Rapamycin and bisindolylmaleimide (BIM) were from Calbiochem (La Jolla, CA). SB-203580 was a generous gift from Dr. John Lee (SmithKline Beecham Pharmaceuticals, King of Prussia, PA). All electrophoresis and immunoblotting reagents were purchased from Bio-Rad (Mississauga, ON, Canada). All other reagents were of the highest analytical grade.
Cell culture and incubations.
A clonal cell line of L6 rat skeletal muscle cells selected for high fusion potential was grown in α-MEM containing 2% FBS; these cells were allowed to fuse and differentiate as previously described (29). Cultures were studied at >90% maximal fusion into fully differentiated myotubes. For total membrane preparation, L6 myotubes were incubated in the presence or absence of insulin (100 nM) for a total of 18 h. Incubation media were replaced every 5 h. Where indicated, the cells were incubated in the presence of 100 nM wortmannin or 20 ng/ml rapamycin for the entire period that insulin was present. For the kinetic analysis of the activation of p70 S6 kinase, the cells were serum deprived in 0.1% FBS-α-MEM for 12 h and stimulated with 100 nM insulin for the indicated time. For PI 3-kinase assay, the serum-deprived cells were stimulated with insulin as indicated in figure legends. All inhibitors were added 30 min before insulin stimulation and were present throughout the incubations.
Isolation of total membranes.
Total membranes of L6 myotubes were prepared as previously described (28). Briefly, cells were rinsed twice with cold homogenization buffer [250 mM sucrose, 20 mM HEPES (pH 7.4), 5 mM NaN3, and 2 mM EGTA]. Cell monolayers were scraped into cold homogenization buffer containing 1 μM leupeptin, 1 μM pepstatin, 10 μM E-64, and 200 μM phenylmethylsulfonyl fluoride (PMSF), homogenized with 20 strokes (Dounce type A homogenizer), and then centrifuged at 700g for 5 min at 4°C. The supernatant from this low-speed spin was then centrifuged for 1 h at 190,000 g to obtain total membranes. Immunoblotting was carried out as previously described (28).
Preparation of cell extracts for p70 S6 kinase or PI 3-kinase assays.
For p70 S6 kinase activity, L6 myotubes grown in 10-cm dishes were washed twice with ice-cold PBS and lysed in 1 ml ofbuffer A [50 mM HEPES (pH 7.5), 150 mM NaCl, 20 mM β-glycerophosphate, 10 mM EDTA, 10 mM sodium pyrophosphate, 100 mM NaF, 1 mM Na3VO4, and 1% (vol/vol) NP-40] containing a mixture of protease inhibitors (1 μM leupeptin, 1 μM pepstatin A, 10 μM E-64, and 200 μM PMSF). After 15 min of slow agitation and centrifugation (15,000g for 15 min), the supernatant was subjected to immunoprecipitation. For PI 3-kinase activity, cell extracts were prepared the same way except for usingbuffer D [20 mM Tris (pH 7.5), 137 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, 100 mM Na3VO4, 1% (vol/vol) NP-40, and 10% (vol/vol) glycerol] and the same mixture of protease inhibitors.
Immunoprecipitation and assay of p70 S6 kinase activity.
p70 S6 kinase was immunoprecipitated by using 0.5 ml of cell extract and 1 μg of a rabbit polyclonal p70 S6 kinase antibody. The p70 S6 kinase immunocomplex was washed three times withbuffer B [50 mM Tris acetate (pH 8), 50 mM NaF, 5 mM sodium pyrophosphate, 5 mM β-glycerophosphate, 1 mM Na3VO4, 1 mM EDTA, 1 mM EGTA, 10 nM okadaic acid, and 0.1% (vol/vol) β-mercaptoethanol], including all the protease inhibitors used above, and twice with buffer C[20 mM MOPS (pH 7.2), 25 mM β-glycerophosphate, 5 mM EGTA, 2 mM EDTA, 20 mM MgCl2, 2 mM Na3VO4, and 1 mM dithiothreitol (DTT)]. p70 S6 kinase activity was assayed essentially as described (24) in a final volume of 50 μl ofbuffer C containing 1 μM PKA and PKC inhibitor peptides, 0.2 mM S6 peptide, and 0.25 mM Mg-[γ-32P]ATP at 30°C for 10 min. Aliquots (30 μl) were transferred onto Whatman p81 filter papers and washed three times for 15 min with 175 mM phosphoric acid (35). 32P incorporated into the S6 peptide was measured by liquid scintillation counting. One unit of protein kinase activity corresponds to 1 μM of32P incorporated into the substrate peptide under the assay conditions.
Immunoprecipitation and assay of PI 3-kinase activity.
PI 3-kinase activity was measured on IRS-1 immunoprecipitates as described (41). Immunoprecipitation was performed as described above but using anti-IRS-1-antibody instead of anti-p70 S6 kinase antibody. The immunoprecipitates were washed three times withwash buffer 1 [PBS containing 1% (vol/vol) NP-40 and 100 μM Na3VO4], three times with wash buffer 2[100 mM Tris (pH 7.5) containing 500 mM LiCl and 100 μM Na3VO4], and twice with wash buffer 3 [10 mM Tris (pH 7.5) containing 100 mM NaCl, 1 mM EDTA, and 100 μM Na3VO4]. The pellets were resuspended in 50 μl of 10 mM Tris (pH 7.5), 100 mM NaCl, 1 mM EDTA, 100 μM Na3VO4, 10 μl of 100 mM MgCl2, and 10 μl of PI (2 mg/ml) in 10 mM Tris (pH 7.5) and 1 mM EGTA. The reaction was initiated by the addition of 5 μl of 440 μM ATP containing 10 μCi of [γ-32P]ATP. After 10 min at 30°C, the reaction was terminated by the addition of 20 μl of 8 M HCl and 180 μl of CHCl3-methanol (1:1 vol/vol). The samples were centrifuged for 5 min at maximal speed in a microcentrifuge, and 50 μl of the lower organic phase were removed and applied to a potassium oxalate (1%)-pretreated silica gel 60 TLC plate that had been prebaked for at least 1 h. Lipids were separated by TLC using CHCl3-CH3OH-H2O-NH4OH (60:47:11:3:2) as the running solvent. The detection and quantitation of [32P]PI3P on the TLC plates were done using a Molecular Dynamics Phosphorimager System (Sunnyvale, CA).
Immunoprecipitation and assay of Akt1 protein kinase activity.
Immunoprecipitation of Akt1 and kinase assay were performed as described (23) with modifications. Anti-Akt1 antibody was precoupled to a mixture of protein A- and protein G-Sepharose beads by incubating 2 μg of antibody per condition with 20 μl of protein A-Sepharose (100 mg/ml) and 20 μl of protein G-Sepharose (100 mg/ml) for a minimum of 2 h. These anti-Akt1-bead complexes were washed twice with ice-cold PBS and once with ice-cold lysis buffer. Akt1 was immunoprecipitated by incubating 200 μg of total cellular protein with the anti-Akt1-bead complex for 2–3 h under constant rotation (4°C). Akt1 immunocomplex was isolated and washed four times with 1 ml of wash buffer [25 mM HEPES (pH 7.8), 10% glycerol (vol/vol), 1% Triton X-100 (vol/vol), 0.1% BSA (vol/vol), 1 M NaCl, 1 mM DTT, 1 mM PMSF, 1 μM microcystin, and 100 nM okadaic acid] and twice with 1 ml kinase buffer [50 mM Tris ⋅ HCl (pH 7.5), 10 mM MgCl2, and 1 mM DTT]. This was then incubated under constant agitation for 10 min at 30°C with 30 μl of reaction mixture (kinase buffer containing 5 μM ATP, 2 μCi of [γ-32P]ATP, and 100 μM Crosstide). After the reaction, 30 μl of the supernatant were transferred onto Whatman p81 filter paper and treated as described above for p70 S6 kinase assay.
Statistical analysis.
Autoradiograms of X-ray films exposed to produce bands within the linear range for quantitation were scanned and quantitated by use of the computer software NIH Image. Statistical analysis was performed using the ANOVA test (Fisher, multiple comparisons). For analysis of PI 3-kinase, Akt1, and p70 S6 kinase activities, the Student's pairedt-test was applied.
Time course of p70 S6 kinase activation by insulin and effect of inhibition of PI 3-kinase.
To understand the relationship between PI 3-kinase and the activation of p70 S6 kinase, we examined the effect of wortmannin on insulin-induced activation of the latter. Previous studies advocating the positioning of PI 3-kinase upstream of p70 S6 kinase measured the activity of p70 S6 kinase after only a 5-min stimulation of cells with growth factors (8). To obtain a more detailed description of the regulation of p70 S6 kinase by insulin, we used an in vitro kinase assay to analyze the effect of wortmannin treatment on p70 S6 kinase catalytic activity in L6 skeletal muscle cells. L6 myotubes were pretreated for 30 min with 100 nM wortmannin before the addition of 100 nM insulin for 5–30 min. p70 S6 kinase was immunoprecipitated, and the kinase activity associated with the immunocomplex was determined by using an exogenous S6 peptide as substrate (a 9-amino acid peptide derived from ribosomal S6 protein). Preliminary experiments confirmed the linearity of the assay within the time frame used (data not shown). As shown in Fig.1 A, insulin increased p70 S6 kinase activity rapidly, reaching a peak in 10 min and remaining elevated for at least 30 min. Although wortmannin (100 nM) completely abrogated insulin-stimulated p70 S6 kinase activity at 5 min, insulin treatment gradually stimulated p70 S6 kinase activity after this time in the continued presence of wortmannin. In two independent experiments, insulin was not able to fully restore the magnitude of p70 S6 kinase activity that was observed in the absence of wortmannin for up to 60 min (data not shown).
We also tested the ability of 5 μM LY-294002, another inhibitor of PI 3-kinase, to inhibit the first (5 min) and second (30 min) phases of p70 S6 kinase activation by insulin. In cells treated with insulin for 5 min in the presence of 5 μM LY-294002 or 100 nM wortmannin for comparison, p70 S6 kinase activity was completely inhibited by both agents (Fig. 1 B), confirming the involvement of PI 3-kinase. However, in cells treated with insulin for 30 min in the continued presence of 5 μM LY-294002 or 100 nM wortmannin, 34 ± 13 or 65 ± 8%, respectively, of insulin-stimulated p70 S6 kinase activity was still measurable.
To determine whether PI 3-kinase was inhibited by wortmannin under these conditions, cells pretreated for 30 min with or without 100 nM wortmannin were stimulated with insulin for 5 or 30 min. IRS-1-associated PI 3-kinase activity was then determined. Insulin (5 min) stimulated PI 3-kinase activity by 34 ± 6.2-fold above control, and in the presence of wortmannin, insulin-stimulated PI 3-kinase activity was 3.1 ± 1.4-fold above control (P < 0.05,n = 5). Wortmannin also inhibited PI 3-kinase activity stimulated by 30 min of insulin treatment (insulin: 11.2-fold above control; wortmannin + insulin: 0.9-fold above control;n = 1).
Dose-dependent effect of wortmannin on p70 S6 kinase activation.
To confirm the existence of a PI 3-kinase-dependent pathway and a PI 3-kinase-independent pathway leading to the activation of p70 S6 kinase, we examined the dose-dependent inhibition of p70 S6 kinase activity by wortmannin on the first (5 min) and second (30 min) phases of activation by insulin. L6 myotubes were treated with 100 nM insulin for 5 or 30 min in the presence of various concentrations of wortmannin, and p70 S6 kinase activity was then assayed. In cells that were treated with insulin for 5 min, wortmannin inhibited insulin-stimulated p70 S6 kinase activity, with an IC50 of ∼20 nM (Table1). In contrast, the IC50 of wortmannin for the inhibition of the second phase (30 min) of activation of p70 S6 kinase stimulated was ∼150 nM (Table 1). This concentration of wortmannin is 5- to 15-fold higher than that for inhibition of class IA PI 3-kinases (11, 43). These results suggest that insulin stimulated the activation of p70 S6 kinase, with two phases having distinct wortmannin sensitivities.
Time course of Akt1 activation by insulin and inhibition by wortmannin.
Overexpression of Akt was shown to activate p70 S6 kinase independent of any stimulus (4). To determine whether Akt could mediate the second phase (30 min) of insulin-stimulated p70 S6 kinase activation that has a reduced sensitivity to wortmannin, we utilized an in vitro kinase assay to investigate the time course of activation of Akt1 by insulin along with the effect of wortmannin. L6 myotubes were treated for 5–30 min with 100 nM insulin in the absence or presence of 100 nM wortmannin. Akt1 was immunoprecipitated, and the kinase activity associated with the immunocomplex was determined by using an exogenous substrate (Crosstide, a peptide derived from region of GSK-3 phosphorylated by Akt). Insulin rapidly stimulated Akt1 activity within 5 min (Fig. 2), and this level of stimulation remained elevated throughout the entire period investigated. Maximal activation was achieved between 10 and 20 min. Wortmannin pretreatment completely prevented the activation of Akt1 by insulin at all time points investigated (Fig. 2), unlike the results obtained for p70 S6 kinase (Fig. 1). Inhibition of insulin-stimulated Akt1 activity by wortmannin is consistent with Akt1 being a downstream target of PI 3-kinase. Because activation of Akt1 by insulin was prevented by wortmannin, we conclude that Akt1 does not play a role in the second phase (30 min) of activation of p70 S6 kinase.
Analysis of second phase of p70 S6 kinase activation by insulin.
The observation that insulin can activate p70 S6 kinase by a PI 3-kinase-independent pathway prompted us to examine other signaling pathway(s) that may mediate this activation of p70 S6 kinase. L6 myotubes were treated for 30 min with 100 nM insulin in the absence or presence of inhibitors of two insulin-stimulated kinases that have been implicated in the regulation of p70 S6 kinase: mitogen-activated protein kinase (MAPK) (14) and PKC (38). The results are shown in Table2. As expected, rapamycin completely abrogated insulin-stimulated p70 S6 kinase activity (1 ± 0.1% of control, n = 3). In concert with the results in Fig. 1 A, wortmannin only partially inhibited the stimulation of p70 S6 kinase by insulin (allowing 65 ± 8% of maximal stimulation,n = 3). BIM (10 μM), an inhibitor of all PKC isoforms at this concentration (26), had no effect on insulin-stimulated p70 S6 kinase activity (Table 2; 106 ± 17% of control, n = 3). This suggests that insulin does not utilize the PKC pathway to stimulate p70 S6 kinase. Similarly, the MAPK/extracellular signal-regulated protein kinase kinase (MEK) inhibitor PD-098059 (10 μM) had no effect on p70 S6 kinase activity (91 ± 5% of control). p38 MAPK, a stress-activated MAPK, has been shown to be activated by insulin in 3T3-L1 adipocytes and fibroblasts (6), and its phosphorylation is stimulated by insulin in L6 cells (40, 42). The specific inhibitor of p38 MAPK, SB-203580 (25), had no inhibitory effect on insulin-stimulated p70 S6 kinase activity (133 ± 44% of control, n= 3), indicating that p38 MAPK is not necessary for the regulation of p70 S6 kinase by insulin.
Wortmannin does not inhibit insulin-stimulated biosynthesis of GLUT-1.
We have previously demonstrated that insulin-induced synthesis of GLUT-1 protein is dependent on the mTOR-p70 S6 kinase signaling pathway in L6 myotubes (39, 40). We therefore investigated which of the two phases of activation of p70 S6 kinase by insulin participates in this end-point response. L6 myotubes were treated with or without 100 nM insulin in the absence or presence of 100 nM wortmannin or 20 ng/ml rapamycin for a total of 18 h. To ensure the stability and bioavailability of wortmannin during the 18-h incubation period, we replaced the incubation medium with medium containing freshly added wortmannin every 5 h. Total membranes were then prepared as described under materials and methods. As shown in Fig. 3 Aand quantified in Fig. 3 B, insulin elicited a significant increase in GLUT-1 protein immunoreactivity above control (1.8 ± 0.6-fold, n = 3, P < 0.05). Consistent with our previous report (39), this increase was completely abolished by 20 ng/ml rapamycin (data not shown). Here we show that, in the presence of wortmannin, insulin still elicited a significant increase in GLUT-1 protein content above control (1.7 ± 0.2-fold,n = 3,P < 0.05). In the absence of insulin, the levels of GLUT-1 were not altered by either wortmannin or rapamycin treatment. After a 5-h pretreatment of L6 myotubes with 100 nM wortmannin, a 5-min insulin challenge was unable to stimulate PI 3-kinase activity associated with IRS-1 immunoprecipitates (insulin: 6.5-fold above control; insulin + wortmannin: 0.9-fold above control). This underscores that wortmannin was still capable of completely inhibiting PI 3-kinase activity 5 h after its addition to cells. Taken together, these results suggest that wortmannin-sensitive PI 3-kinases are not essential for insulin stimulation of GLUT-1 protein synthesis in L6 skeletal muscle cells.
Two pathways leading to p70 S6 kinase activation.
Here we show that activation of p70 S6 kinase by insulin in muscle cells occurs in two phases, distinguishable by their different sensitivity to inhibitors of PI 3-kinase. The IC50 of inhibition of the first phase by wortmannin (20 nM) is consistent with that for inhibition of class IA PI 3-kinases (11, 44). In vitro, the IC50 of wortmannin for the inhibition of p85/p110 PI 3-kinases is 2 nM (36, 43) and in intact cells is 10–30 nM (43). On the other hand, the higher IC50 value of wortmannin for inhibition of the second phase (150 nM) suggests that class IA PI 3-kinases are not involved and that wortmannin targets another upstream activator, possibly mTOR. This notion is supported by two recent reports that demonstrate that wortmannin irreversibly inhibits the serine-specific autokinase activity (3) and protein kinase activity (2) of mTOR with IC50 of ∼200 nM. The higher sensitivity of the second phase of p70 S6 kinase activation to LY-294002 than to wortmannin also supports this hypothesis, since Brunn et al. (3) demonstrated that the IC50 of LY-294002 for inhibition of both mTOR and PI 3-kinase is 5 μM. The yeast and mammalian TOR proteins are now recognized as members of a growing family of high-molecular-weight kinases whose catalytic domains resemble those of PI 3-kinases (19) and are necessary for the activation of p70 S6 kinase by all known stimuli, including insulin (9, 32, 34). Although mTOR protein is upregulated as 3T3-L1 fibroblasts differentiate into the more insulin-responsive adipocytes (46), it is unknown how insulin regulates the kinase activity of this protein.
The protein kinase activity of mTOR is stimulated by serum in vivo, and this precedes the activation of p70 S6 kinase (5). Full activation of p70 S6 kinase by growth factors is achieved with phosphorylation of three critical sites (Thr229, Thr389, and Ser404) (16, 31), and phosphorylation of these sites is prevented by both wortmannin and rapamycin (16, 31). The high sensitivity of Thr389 phosphorylation to wortmannin suggests that this site is phosphorylated by an as yet unidentified PI 3-kinase-dependent kinase (12, 33). On the other hand, the high sensitivity of Thr389phosphorylation to rapamycin may be explained by the recent observation that mTOR phosphorylates this site in vitro. Taken together, these observations suggest that Thr389could be phosphorylated by two different kinases that may be able to functionally substitute for each other. We therefore propose a model in which insulin stimulates p70 S6 kinase via two pathways. The first is rapid, completely dependent on PI 3-kinase and mTOR. In the absence of functional class IA PI 3-kinases (i.e., in presence of 100 nM wortmannin), mTOR may unilaterally mediate the phosphorylation of Thr389, leading to the second phase activation of p70 S6 kinase, which is the PI 3-kinase-independent pathway. Alternatively, the second phase of activation of p70 S6 kinase could be mediated by PI 3-kinases that are less sensitive to wortmannin, such as the class II PI 3-kinases (11, 44). It is unknown at present whether any of these PI 3-kinases are activated by insulin.
Analysis of second phase of activation of p70 S6 kinase.
To further understand the stimulation of p70 S6 kinase by insulin, we sought to define the signaling mechanism(s) of the PI 3-kinase-independent pathway. We utilized specific pharmacological inhibitors that act on kinases known to be stimulated by insulin and implicated in the regulation of p70 S6 kinase. EGF stimulates p70 S6 kinase in Swiss 3T3 fibroblasts in a biphasic manner (38), with the second phase requiring conventional isoforms of PKC (cPKC) (37, 38). However, PDGF and insulin stimulate p70 S6 kinase in the same cells in a cPKC-independent manner (38). Here, by utilizing BIM at a concentration known to inhibit all isoforms of PKC (26), we demonstrate that none of the known PKC isoforms is involved in the second, PI 3-kinase-independent phase of insulin-stimulated p70 S6 kinase activation. MAPK was originally thought to be the proline-directed kinase that phosphorylates the COOH-terminal autoinhibitory region of p70 S6 kinase (30). However, this remains debatable, since other studies have reported results to the contrary (27). Inhibition of the MAPK pathway with the MEK inhibitor, PD-098059 (1), had no effect on p70 S6 kinase activation by insulin (30-min stimulation). It has also been suggested that the stress-activated MAPK, p38 MAPK, mediates the activation of p70 S6 kinase by sodium arsenite in cardiomyocytes (45). Because this kinase is also activated by insulin (6, 40, 42), we tested its involvement in insulin-stimulated p70 S6 kinase activity. The specific p38 MAPK inhibitor, SB-203580 (25), had no effect on insulin-induced p70 S6 kinase activity (30-min stimulation).
Role of Akt1 in activation of p70 S6 kinase.
Akt has recently been identified as a direct downstream substrate of PI 3-kinase in many growth factor signaling pathways (18). However, Akt could be activated by basic fibroblast growth factor without prior activation of PI 3-kinase (21), whereas overexpression of Akt in cells suggested that it had a role in the activation of p70 S6 kinase (4). To determine whether the kinetics of activation of Akt matched those of the activation of p70 S6 kinase, we first looked at the effect of wortmannin on the activation of Akt1 by insulin. Activation of Akt1 was fully inhibited by 100 nM wortmannin under conditions in which only 35% of insulin-stimulated p70 S6 kinase was inhibited. Thus Akt1 activation by insulin could not support the latent activation of p70 S6 kinase in the face of complete inhibition of PI 3-kinase. Together with the results presented here, this suggests that if Akt1 is involved in the regulation of p70 S6 kinase, it can mediate only the first phase of p70 S6 kinase activation.
Akt2 and Akt3 are two additional isoforms of Akt that have been suggested to be downstream of PI 3-kinase (13). No detailed time course showing activation of these two isoforms has been reported. Hence, we do not know whether the kinetics of Akt2 and Akt3 activation match those for activation of p70 S6 kinase. More detailed studies of Akt2 and Akt3 are required before we can assign a role for either one in the regulation of p70 S6 kinase. However, we can speculate that these two isoforms may participate in the first phase of activation of p70 S6 kinase because their activation is PI 3-kinase dependent.
GLUT-1 protein synthesis occurs via second phase of p70 S6 kinase activation.
Finally, we demonstrate that wortmannin was unable to block insulin-stimulated GLUT-1 protein synthesis in L6 muscle cells, whereas rapamycin did. It is unlikely that degradation of wortmannin during the incubation caused reactivation of PI 3-kinase, since repetitive addition of wortmannin every 5 h was still effective in preventing the rapid stimulation of PI 3-kinase by insulin. A similar protocol for prolonged treatment with wortmannin was shown to inhibit differentiation of L6E9 cells (20). Wortmannin covalently modifies Lys802 in the lipid kinase domain of the p110 catalytic subunit, resulting in the irreversible inhibition of PI 3-kinase activity (47). Thus PI 3-kinase is not required for insulin to upregulate GLUT-1 protein, and it is likely that the second phase of activation of p70 S6 kinase is sufficient to upregulate GLUT-1 protein content.
In summary, the activation of p70 S6 kinase by insulin in L6 skeletal muscle cells is composed of two separable components: an early phase dependent on class IA PI 3-kinase and possibly Akt1, and a second phase independent of class IA PI 3-kinases (Fig.4). The latter phase does not involve PKC, MEK, or p38 MAPK. In addition, insulin upregulates GLUT-1 protein despite complete inhibition of PI 3-kinase and Akt1 activation with wortmannin. Because the second phase of activation of p70 S6 kinase remains sensitive to higher levels of wortmannin, it is possible that PI 3-kinase-related mTOR or another PI 3-kinase enzyme is involved in the maintenance of insulin-stimulated p70 S6 kinase activity and GLUT-1 protein biosynthesis.
We thank Dr. Philip J. Bilan for critical reading of this paper.
Address for reprint requests: A. Klip, Programme in Cell Biology, The Hospital for Sick Children, 555 University Ave., Toronto, ON, Canada M5G 1X8.
We are grateful to Dr. A. R. Saltiel for the supply of PD-098059, Dr. J. C. Lee for the supply of SB-203580, Dr. M. Caplan for the anti-Na+-K+-ATPase α1-antibody, and Dr. R. Roth for advice on the Akt activity assay.
This work was supported by a grant from the Canadian Diabetes Association to A. Klip. R. Somwar and S. Sumitani contributed equally to the present work.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
- Copyright © 1998 the American Physiological Society


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Getting Well Again: The Bestselling Classic about the Simontons' Revolutionary Lifesaving Self- Awareness Techniques (Mass Market Paperbound)
Based on the Simontons' experience with hundreds of patients at their world-famous Cancer Counseling and Research Center, Getting Well Again introduces the scientific basis for the "will to live." In this revolutionary book the Simontons profile the typical "cancer personality": how an individual's reactions to stress and other emotional factors can contribute to the onset and progress of cancer -- and how positive expectations, self-awareness, and self-care can contribute to survival. This book offers the same self-help techniques the Simonton's patients have used to successfully to reinforce usual medical treatment -- techniques for learning positive attitudes, relaxation, visualization, goal setting, managing pain, exercise, and building an emotional support system.
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Introduction to Cheryl Slavin, Author of 'Anxiety-Shmanxiety'
I'm Cheryl Slavin, the new co-author of Anxiety-Schmanxiety. I have had symptoms of my primary disorder, obsessive-compulsive disorder (OCD), since I was five years old. Generalized anxiety disorder (GAD) was also an early companion. As the sixth grade's favorite dodgeball target, I was bullied in elementary school and developed social anxiety disorder (SAD). I am also afflicted with bipolar disorder.


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(Rebel Belle #1)
Release Date: April 8, 2014
Publisher: Putnam Juvenile; 384 pages
Harper Price, peerless Southern belle, was born ready for a Homecoming tiara. But after a strange run-in at the dance imbues her with incredible abilities, Harper's destiny takes a turn for the seriously weird. She becomes a Paladin, one of an ancient line of guardians with agility, super strength and lethal fighting instincts.
Just when life can't get any more disastrously crazy, Harper finds out who she's charged to protect: David Stark, school reporter, subject of a mysterious prophecy and possibly Harper's least favorite person. But things get complicated when Harper starts falling for him--and discovers that David's own fate could very well be to destroy Earth.
With snappy banter, cotillion dresses, non-stop action and a touch of magic, this new young adult series from bestseller Rachel Hawkins is going to make y'all beg for more.
If you're in the mood for something light and funny, let me tell you something, Rebel Belle is the book for you! Of the books I have read this year, this one has probably been the one that I've had the most fun reading. The book opens full of action. When I read the first chapter, my first thought was, "Now that's how you open a book!" I was instantly hooked.
Rebel Belle is the story of Harper Price. A normal high school girl who is a bit of an overachiever. She's a cheerleader, president of numerous clubs at her school, and she is also in the running to be her class valedictorian. You can't help but love Harper. She's just so bubbly and energetic. Then one day something so normal as going to the restroom to touch up your lip gloss, causes her to enter a world filled with magic and action and adventure. I think this was one of the things that I loved the most. Harper truly was a typical teenager. It wasn't your usual the lead was always destined to have powers, that aren't awakened until a certain age or something along those lines. Harper was at the wrong place at the wrong time.
And to make things interesting we're introduced to David. David is pretty much Harper's nemesis. You know that person you went to school who you rival in pretty much everything. That's what David is to Harper. I seriously loved their relationship. There is just something about a love/hate relationship that really intrigues me. There were times were I was seriously screaming, "Just kiss already!" Watching their relationship evolve was interesting to read as well. Especially since the two of them are put in a pretty interesting position.
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If you're looking for a light and quick read, I would definitely recommend Rebel Belle. Even if you are more of a contemporary reader, I would still recommend it. Rebel Belle has a bit of everything, so fans of multiple genres would enjoy it!


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Movies Worth Watching
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The Big Lebowski (1998)
"The Dude" Lebowski, mistaken for a millionaire Lebowski, seeks restitution for his ruined rug and enlists his bowling buddies to help get it. (117 mins.)
Director: Joel Coen
Stars: Jeff Bridges, John Goodman, Julianne Moore, Steve Buscemi
Snatch (2000)
Unscrupulous boxing promoters, violent bookmakers, a Russian gangster, incompetent amateur robbers, and supposedly Jewish jewelers fight to track down a priceless stolen diamond. (104 mins.)
Director: Guy Ritchie
Stars: Jason Statham, Brad Pitt, Benicio Del Toro, Dennis Farina
Hesher (2010)
A young boy has lost his mother and is losing touch with his father and the world around him. Then he meets Hesher who manages to make his life even more chaotic. (106 mins.)
Director: Spencer Susser
Django Unchained (2012)
With the help of a German bounty hunter, a freed slave sets out to rescue his wife from a brutal Mississippi plantation owner. (165 mins.)
American History X (1998)
A former neo-nazi skinhead tries to prevent his younger brother from going down the same wrong path that he did. (119 mins.)
Director: Tony Kaye
Gladiator (2000)
When a Roman General is betrayed, and his family murdered by an emperor's corrupt son, he comes to Rome as a gladiator to seek revenge. (155 mins.)
Director: Ridley Scott
Shutter Island (2010)
In 1954, a U.S. Marshal investigates the disappearance of a murderer, who escaped from a hospital for the criminally insane. (138 mins.)
Director: Martin Scorsese
Requiem for a Dream (2000)
The drug-induced utopias of four Coney Island people are shattered when their addictions run deep. (102 mins.)
Director: Darren Aronofsky
Inglourious Basterds (2009)
In Nazi-occupied France during World War II, a plan to assassinate Nazi leaders by a group of Jewish U.S. soldiers coincides with a theatre owner's vengeful plans for the same. (153 mins.)
Stars: Brad Pitt, Diane Kruger, Eli Roth, Mélanie Laurent
Pulp Fiction (1994)
The lives of two mob hit men, a boxer, a gangster's wife, and a pair of diner bandits intertwine in four tales of violence and redemption. (154 mins.)
Fight Club (1999)
An insomniac office worker, looking for a way to change his life, crosses paths with a devil-may-care soap maker, forming an underground fight club that evolves into something much, much more. (139 mins.)
Director: David Fincher
Stars: Brad Pitt, Edward Norton, Meat Loaf, Zach Grenier
Oldboy (2003)
After being kidnapped and imprisoned for fifteen years, Oh Dae-Su is released, only to find that he must find his captor in five days. (120 mins.)
Director: Chan-wook Park
Stars: Min-sik Choi, Ji-tae Yu, Hye-jeong Kang, Dae-han Ji
Fargo (1996)
Jerry Lundegaard's inept crime falls apart due to his and his henchmen's bungling and the persistent police work of the quite pregnant Marge Gunderson. (98 mins.)
Director: Joel Coen
The Devil's Rejects (2005)
The murderous, backwoods Firefly family take to the road to escape a vengeful police force which is not afraid of being as ruthless as their target. (107 mins.)
Director: Rob Zombie
Donnie Darko (2001)
A troubled teenager is plagued by visions of a man in a large rabbit suit who manipulates him to commit a series of crimes, after he narrowly escapes a bizarre accident. (113 mins.)
Director: Richard Kelly
Office Space (1999)
Three company workers who hate their jobs decide to rebel against their greedy boss. (89 mins.)
Director: Mike Judge
Stars: Ron Livingston, Jennifer Aniston, David Herman, Ajay Naidu
American Beauty (1999)
A sexually frustrated suburban father has a mid-life crisis after becoming infatuated with his daughter's best friend. (122 mins.)
Director: Sam Mendes
Stars: Kevin Spacey, Annette Bening, Thora Birch, Wes Bentley
Kill Bill: Vol. 1 (2003)
The Bride wakens from a four-year coma. The child she carried in her womb is gone. Now she must wreak vengeance on the team of assassins who betrayed her - a team she was once part of. (111 mins.)
Stars: Uma Thurman, David Carradine, Daryl Hannah, Michael Madsen
" and volume 2 " - chrispraill
Man on Fire (2004)
In Mexico City, a former assassin swears vengeance on those who committed an unspeakable act against the family he was hired to protect. (146 mins.)
Director: Tony Scott
The Godfather (1972)
The aging patriarch of an organized crime dynasty transfers control of his clandestine empire to his reluctant son. (175 mins.)
Director: Francis Ford Coppola
Stars: Marlon Brando, Al Pacino, James Caan, Diane Keaton
" and part 2 " - chrispraill
Scarface (1983)
In Miami in 1980, a determined Cuban immigrant takes over a drug cartel and succumbs to greed. (170 mins.)
Director: Brian De Palma
Thank You for Smoking (2005)
Satirical comedy follows the machinations of Big Tobacco's chief spokesman, Nick Naylor, who spins on behalf of cigarettes while trying to remain a role model for his twelve-year-old son. (92 mins.)
Director: Jason Reitman
Stars: Aaron Eckhart, Cameron Bright, Maria Bello, Joan Lunden
American Gangster (2007)
In 1970s America, a detective works to bring down the drug empire of Frank Lucas, a heroin kingpin from Manhattan, who is smuggling the drug into the country from the Far East. (157 mins.)
Director: Ridley Scott
Reservoir Dogs (1992)
After a simple jewelry heist goes terribly wrong, the surviving criminals begin to suspect that one of them is a police informant. (99 mins.)
Stars: Harvey Keitel, Tim Roth, Michael Madsen, Chris Penn
American Psycho (2000)
A wealthy New York investment banking executive, Patrick Bateman, hides his alternate psychopathic ego from his co-workers and friends as he delves deeper into his violent, hedonistic fantasies. (102 mins.)
Director: Mary Harron
Stars: Christian Bale, Justin Theroux, Josh Lucas, Bill Sage
Lawless (2012)
Set in Depression-era Franklin County, Virginia, a trio of bootlegging brothers are threatened by a new special deputy and other authorities angling for a cut of their profits. (116 mins.)
Director: John Hillcoat
Stars: Tom Hardy, Shia LaBeouf, Guy Pearce, Jason Clarke


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'42' ... we are a playoff team. I know that. I felt that way even when we were 1-7 in the preseason. I want more though, and we can get more than just playoffs if we get rid of a few loose ends. David West, Thornton, Green, J. Smith, Okafor, Ariza, and Belinelli made Chris Paul look like the 2nd best PG in the NBA tonight. Deron William's team is just better ... so it's easy for him to rack up assist and have a good game. The league doesn't see it that way. They believe Williams is better by way of stats. I'm not suggesting we get rid of all of the names that I mentioned, but they have to be 'MONSTERS' when they play these Elite teams. I'm tired of people saying our record is a fluke ... but ... we proved them right tonight! I hate proving others right when I truly believe they aren't. Chris Paul still has to work too damn hard against the Jazz, and maybe even the Lakers, Celtics, and Magic still! I'm frustrated because I see something in this team ... they can go deep into the playoffs if they were a 'wee-bit' tougher. David West gets killed by tougher players all the time, and I'm tired of seeing that. I thought it would be different this year, but he is still soft ... to a degree ... and that degree is the Elite Teams! Maybe I'm overreacting about Marcus Thornton ... but atleast stop calling him 'Buckets' ... see, that's just plain-old irritating ... especially when he doesn't hit any. L_Reazy
Some girl who's hot for Thorton came up with the Buckets nickname. Definitely wasn't my first choice, and with the way he's playing now, he wouldn't be my third.
I think we're on the same page about the team. There's are a couple of threads in the forum you may enjoy that are focused on these issues. You may find them more satisfying since they are dedicated to a topic, so the discussion won't be strewn throughout various game day threads here in the posts.