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About Canada
Canada ranks as the best place on earth and we're proud!
Compared with other industrialized countries, Canada is the clear winner in many areas. Not only is Canada the world's most diverse and tolerant society, but we're a uniquely privileged nation wealthier, healthier, and happier than practically any other place on Earth. Canada offers the highest quality of life and abundant opportunities for all. Canada is truly a great country for immigrants and offers spectacular natural beauty, a healthy environment, cultural diversity, lively communities and a strong economy.
"We're wealthier than Americans, we live longer than the Swedes, we're more industrious than the Germans, we have more lovers than the Italians, we eat better than the French and we have more TVs than the Japanese. In so many areas—the economy, health, education, public safety, and living standard—the numbers, it seems, back up what we've always quietly believed deep in our patriotic hearts. Sorry to brag, but it looks like Canada is the best place on earth."
- Maclean's magazine, July 6/09
Canada is #1
- Nearly 100% of Canadians feel positive about immigration/newcomers (Ipsos-Reid)
- Canada has the highest per capita immigration rates in the world. While most Canadians accept newcomers, Canadians believe that different groups belong here and that interaction between those groups is a good thing.
- 84% of Canadian residents are happy to be Canadian (Maclean's)
- Canada offers the highest quality of life in the world (United Nations Human Development Index)
- A 2008 Haver Analytics report ranks Canada no. 2 among the top industrialized countries in the world, with a financial net worth per household of US$154,100.
- Canadians have the highest home ownership rates and live in spacious comfort – 77% of Canadian homes have five or more rooms
- The World Economic Forum in 2009 confirmed that Canada boasts the world's strongest financial system
- Canada is the seventh-largest oil producing nation in the world
- Canada has one of the highest life expectancies (average 81.23 yrs)
- Canada provides a free world-class healthcare system to all its citizens
- Canada is one of the safest countries in the world
- HSBC Bank International reported Canada is the friendliest country
- Forbes magazine ranked Canada as the fourth hardest-working country in the developed world
- Canadians are fun and don't take themselves too seriously
- Men's Health magazine (2007) proclaimed Canada as the second-fittest and second most-relaxed country in the world
- Canada has the most educated people per capita in the world. A full 47% of the population proceed to post-secondary school compared with 39% in the US, and 30% in UK.
- Canadians are honest. While corruption is prevalent in other parts of the word, it is practically non-existent in Canada. Internationally, Canadians are recognized for our honesty and integrity.
The Economist magazine pronounced that Canada had become rather cool (hip and open-minded). Canadians are friendly, tolerant and modern with 89% of us fulfilled with our lives as a whole. Canadians are happy, and we can help you enjoy the same benefits and opportunities we have.
About Major Canadian Cities
Welcome to the Province of Ontario
Ontario is home to more than 12 million people and is Canada's most multicultural province where people from 200 countries speaking as many as 130 languages call home. Ontario is a land of opportunity, has one-third of the world's fresh water, and is a hub for manufacturing, finance, tourism and many other industries.
Moving to Toronto
Province: Ontario
- Population of Greater Toronto: about 6 million
- Home to more than 200 ethnicities! Designated world's most ethnically-diverse city (-The United Nations)
- World's fourth-largest capital market and North America's second largest stock exchange
- Ranked safest large metropolitan area in North America (Places Rated Almanac)
- Followed by Vancouver and Montreal, Toronto attracts the highest number of immigrants
- The Toronto Stock Exchange (TSX) is a global leader in the mining, oil and gas sectors, and is the second largest technology market in the world
Moving to British Columbia
The province of British Columbia (BC) is located on Canada's west coast, between the Pacific Ocean and the Rocky Mountains. BC is home to the 2010 Winter Olympics and enjoys spectacular scenery, a robust economy, an educated and skilled population, and an exciting future. Each year BC welcomes more than 40,000 immigrants.
Moving to Vancouver
- Population of Greater Vancouver Area: 2.1 million
- Rated the world's most livable city (The Economist)
- Third-largest metropolitan area in Canada
- The most moderate climate of Canada's major cities
Moving to the province of Alberta
Alberta's booming economy and quality of life is attracting people from across Canada and around the world. Alberta is home to the majestic Rocky Mountains, vast prairies, modern cities and the world's second largest oil reserve. Its education system ranks among the best in the world. Alberta also enjoys low taxes, a driven entrepreneurial spirit and abundant natural resources.
Moving to Calgary
- Population of Calgary: 1.1 million
- Largest city in Alberta, fifth largest city in Canada
- Second largest centre for head offices in Canada
- Alberta has the second largest oil reserves in the world
Moving to the province of Quebec
Quebec is the largest Canadian province and the only one where French is the first language! Quebecers are known for their charisma and joie de vivre. There are over 7 million people, including 700,000 newcomers. Quebec boasts a diversified economy and abundant natural resources. With its own immigration system, Quebec is a place that welcomes immigrants.
Moving to Montreal
- Population of Greater Montreal Area: 3.6 million
- Second largest French-speaking city in the world
- Tenth cleanest city in the world (Forbes)
- Home to Underground City – a climate-controlled maze of 2,000 shops covering 18 miles
Moving to the province of Nova Scotia
Today there are close to 1 million people who call Nova Scotia home. Nova Scotia has one of the fastest-growing economies in Canada based on natural resources, tourism and hospitality, health care and research, manufacturing, and information and computer technology. Immigrants have helped make Nova Scotia a vibrant, dynamic society.
Moving to Halifax
- Atlantic Canada's largest city with a population of 360,000
- One of the world's great natural harbours
- Halifax is a busy Atlantic seaport, and is the economic and cultural hub of Eastern Canada
- Nova Scotia is a remarkably scenic province, renown for its hospitality


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Making FUT Coins Using the 1, 2, 3 Method
The next in our series of posts about making more coins in Fifa Ultimate Team is what we're calling the 1, 2, 3 method. This is a method that is regularly used in Youtube "Road to Glory" videos and is used in order to make coins, attain top quality players and for some, achieve the amazing teams you see in some of their videos.
Step 1:
When searching in the transfer market, You set the minimum bid to 20,000 and then the maximum bid to however many coins you are willing to spend; For this example we'll say 50,000. After that you will set the minimum buy it now to something like 100,000… Doing this will get rid of the cheap players that you're not going to make any profit of. This will also highlight the players that are currently selling for under their buy it now price which means you could pick up a serious bargain.
Step 2:
Next, search through the market to about 2-3 minutes left on the auctions and see if you can spot any bargains or anyone you can potentially make a profit on. You can then add that player to your watch list. Keep doing this until you have about 5-6 players on the list.
Step 3:
Finally, manually search for other people selling that player on the transfer market, and find the lowest available buy it now price. Make a note of what it is, and if the player you have on your watchlist has a lower buy it now price than the other ones on the market, buy it. This is a method that is guaranteed to work every time because you know that you are making profit on every single player you buy. When using this technique, remember to take into account that EA take 5% on every sale in the transfer market and that could be the difference between a profit and a loss.
Getting the search right at the start is the key to this, it will highlight all the top, ordinarily most expensive players selling for a bit less than what they should. This is arguably the best way to turn a profit on the marketplaces built in to FUT, but it is the most time consuming. If you stick with it your team will soon start to benefit from it, and you can sit back and watch the reward.

subset: commoncrawl


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For most of the animal lovers we just don't treat animals as animals as they are more than just pets—they're members of the family. When they pass on, we grieve our deceased pets intensely and their absence leaves a huge hole in our hearts. While they may …Read More »
Reaching Out To The Loved Ones Who Passed Away
Have you ever thought about having a psychic medium perform a reading or afterlife séance for you? Have you felt a bit skeptical about the whole scenario? If you have wanted to connect with someone who is already in the afterlife or hear what is in store for your future …Read More »
How To Move On After Losing Someone
Being cheated on is difficult, having someone leave you is heart breaking but losing a loved one is incredibly painful and moving on from the pain is often even harder. The grieving process does and should take time, and there is no set timetable for getting through it, …Read More »
There Could Be An Angel Near You
Who doesn't want to see angels? Who doesn't want to be guided by angels? Angels have been described in spiritual texts throughout time as strong energy forces that watch over us and guide us when we need a helping hand. When we think of angels, …Read More »
Is It High Time To Contact Your Deceased Loved Ones?
One of our clients, Angela came to our reading with the desire to reach out to her recently deceased mother. Her mother had gone into a coma after a stroke and Angela had sat with her in those final days making amends over some past hurts but her mom never …Read More »
A Paranormal Psychic
As we are living in the modern times, the words "psychic" and "the paranormal" would stirs up all kinds of scary imaginings like ghosts, levitating chairs, things that go bump in the night, etc. However, from a psychic's perspective, it's actually not the least bit unsettling to place the two terms side-by-side. …Read More »
Why Does A Poltergeist Exist?
A Poltergeist or a bad ghost as translated from German is a term used for spiritual being that usually appears to attach themselves to a particular person. In most cases, the person will be either a child or adolescent who is entering or going through puberty but there …Read More »
The Real Reason Why Your Deceased Loved Ones Occur In Your Dream
Losing your loved one can be the hardest experience in life and maybe one of the most traumatic event that someone can go through. After the loss, there can be an enormous feeling of sadness, guilt, and shame around their passing, especially if their death was unexpected …Read More »
Let's The Ghosts Be Gone From Home
Have you ever felt like you're never truly home alone? Did you ever feel that someone is watching you inside your room? Well, you may be sharing your home with spirits. Whether they're compassionate or unscrupulous spirits, you can take steps to set them free. Try these methods …Read More »
Love Beyond Death
"The love of a half dead heart will keep you half alive" We all must pass away sometime—but when we lose someone we love, the pain and sadness we experience are very difficult to bear…. It seems like we are just living for the sake of living but …Read More »


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Free trial Go
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If you require assistance, please call 1-800-513-5778 or email [email protected].
A sales consultant will contact you within a few days to see if you have any questions or are ready to move forward.
Looking for more information now?
Why not learn more about GoCodes features & affordable pricing.
Watch short YouTube videos that demonstrate some of the amazing, easy to use features.
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"Easy-to-use software. Affordable price. Secure, tamper-proof physical stickers with owner information. Locates powered-down assets online or from mobile devices. Tracks any asset, even non-networked assets."
PC Magazine Review, Oct 2015
"GoCodes had the right combination of price and functionality. Having a web portal in which to track our assets is a great convenience."
Marc Mangus, Vice President of Technology, EmLogis
"The system is very user-friendly with an easy-to-understand interface. GoCodes makes it easy to protect our assets in an industry where we have a high risk of losing expensive equipment."
Gary Merrill, EMS Director
"I have fully run through the website and have used the demo version of the software to run a test and see if this is something that might work for my company. I have to say that it's pretty awesome and it's something that can actually grow with a company rather than just being a "one off" sort of thing."
Tom Gilman, The Railroad Group


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Sbb14-Tae Won Chung
- 1 Project Outline
- 2 Organism
- 3 Construction Files
- 4 May 1st and Result
- 5 April 29th and 30th
- 6 April 24th
- 7 April 22nd
- 8 April 17th
- 9 April 15th
- 10 April 10th
- 11 April 8th
- 12 April 3rd
- 13 April 1st, Restarting
- 14 March 13-20th
- 15 March 11th
- 16 March 6th
- 17 March 4th
Project Outline
The purpose of the project is to transform thermostable aminoacyl-tRNA synthetase genes from thermophilic bacteria into E. coli. This involves following steps:
- PCR Cloning of desired genes from bacterial genome.
- Removal of Internal restriction site in case there is internal restriction site.
This is particularly done by splicing with overlap extension (SOE)
- Digesting the PCR product and ligating on to a vector.
- Transforming the plasmid into host cell
- Mini-preping sample colonies of the cell to analyze whether transformation was successful or not.
prolyl-tRNA synthetase
locus tag = TM_0514 length = 1734 bp
>gb|AE000512.1|:540171-541904 Thermotoga maritima MSB8, complete genome TCACCTGACAGCCTCCTTGGGATCGTACTCCCGCTTCATCTTTTCCAGTGTCTCCAGAAGCGCACCGAAA CCGTTTTTGATGTTCACCTTGACGAGTTCTTTCGAATAGCGTTTCTTCAGTTCAACAACACCTTCCTTGA GGGATCTTCCCACGTTTATTCTTATGGGAAAGCCTATGAGATCGGCGTCTTTGAATTTGAAACCAGGCGA GACTTCTCTGTCATCCAGAACAACCTCTTCTCCTTTTTCTGAGAGAACCTGGTAAATCTTCTCACCCACC TGCTTCTGTTCAGCGTCGTTCATGTTCAGAATGTCCACCACAACGGTGTAAGGGGCGATCGAAAGGGGCC AGATCATACCGTTCTCATCGTGGAAATGTTCCACAACAGCCGCCATCGTTCTGGAAACTCCCCAGCCATA ACAGCCCATGATGAAAGGCTTCATCTCACCGTTTTCATCCATGAAGTAGGCCTTCATGGCTTCGGAGTAT TTTGTGCCGAGTTTGAAGATGTGACCAAGCTCTATCCCTTTTGTCGCCTTGAGAGGCTCACCACAGACGG GACAGGGATCACCCTCCACCACCGTTCTCAGATCGTACCACTCGTCCACCTTGAAATCTCTGGGGTGGTT TGCGTTTACGTAGTGCGTATCCTCTTCCATACCCCCAACGACGAAGTTTTTCAGTCCTCTGACGCTGTGA TCGGCCACTTTCTTCACATCCACACCGATGGGTCCAATGAATCCGACGGGAACTCCGAAGTCCTTCAGAA TTTCTTCTGGGGTTGCCATTCTCAGCGACTGATCTTTCAAATGTGCTTTGAGCTTCGCTTCGTTGAGCTC CAGGTCTCCCCTTATAAGTACCATTACGTATCCTTCTCTTCCTTTGTAAAGAAGGGACTTCACAATCTTC GACGGAGGAACACCGAGAAACTCTGAAACTTCCTCGATCGTCTTCACCCCGGGTGTGGGAACCTTCTTGA GGGGTTTCTCTTCTTCCTGTTCCTGAGTGTATTCACCTTTGTATTCGGCTTTTTCGTCGCTTGCCTGGTA GCCGCACTTTTCACAGAAGAGTACGTTCGTCTCTCCTATTTTCGCAGGAACGACGAACTCGTGGGAAGCG TTCCCTCCGATGGCACCCGTTTCTGCCTCTATGATCATGTACCGCACACCAAGCCTTTCCATGATTCGGG AGTACGCTTTTTTGAACTGTTCGTACGTCTCGTCCAGAGATTCCCAGCTTGCGTGAAAGCTGTAAGCGTC CTTCATGATGAATTCTCTCGCCCTGAGAAGGCCGAAGCGTGGTCTGATTTCGTCTCTGTACTTGTTCGCT ATCTGATACAGAGTGAGAGGAAGCTGTTTGTATGAACGAAGTTCGTTCTTCACAAGGTCCGTGACGATCT CCTCGTGCGTGGGACCGAGTGTGAAGTCTCTTTCGTGCCTGTCTTTGAGTTTCATCATTTCGGGACCGTA ATCGTCCCACCTTCCTGACTGTTTCCAGAGTTCCGCAGGTTGAAGGATGGGCATCAGAATTTCCTGTGCC CCTATCCTGTTCATCTCTTCTCGAACTATGTTCTCTATTTTGAGAAGCACTCTTCTTCCTAATGGGAGGT AGGTGTATATACCGGCGGCTACTTTTCTTATGAAGCCTCCTCGAAGAAGATACTCGTGGCTTACTGTCTC AACATCGGAAGGGGTTTCTTTGAGAGTAGGAGCATAGAGGTCTTTCATCCGCAA
glutamyl tRNA-Gln amidotransferase, subunit A
locus tag = TM_1272 length = 1428 bp
>gb|AE000512.1|:1297318-1298745 Thermotoga maritima MSB8, complete genome GTGATCGATTTGGACTTCAGAAAACTCACAATAGAAGAGTGTCTGAAACTTTCCGAAGAAGAAAGGGAGA AACTCCCACAACTTTCCCTGGAAACCATCAAAAGACTCGATCCACACGTGAAGGCGTTCATCTCCGTAAG AGAGAATGTTTCCGTTGAGAAGAAGGGAAAATTCTGGGGAATTCCAGTTGCGATCAAGGACAACATTCTC ACACTGGGCATGAGAACGACCTGTGCCTCCAGGATACTCGAAAACTACGAATCCGTTTTCGATGCCACAG TGGTGAAGAAGATGAAGGAAGCAGGCTTTGTCGTGGTTGGGAAGGCAAACCTCGATGAGTTTGCGATGGG CTCGAGCACCGAAAGATCCGCATTCTTTCCCACAAGAAACCCCTGGGACCTGGAAAGGGTTCCCGGCGGA AGCAGTGGGGGTTCTGCCGCGGCCGTTTCTGCAGGAATGGTGGTGGCAGCACTCGGAAGCGACACGGGAG GCTCTGTGAGACAGCCCGCTTCCCTGTGCGGTGTTGTTGGGTACAAGCCCACCTACGGTCTCGTCTCAAG GTACGGTCTTGTAGCGTTTGCAAGTTCTCTGGACCAGATCGGACCCATCACAAAGACCGTAAGAGACGCT GCAATCCTCATGGAGATCATCTCGGGCCGGGACGAAAACGATGCCACGACGGTGAACAGGAAGGTGGACT TCCTCTCGGAGATAGAAGAGGGAGTCTCCGGCATGAAGTTTGCCGTTCCTGAAGAGATCTACGAACACGA TATAGAAGAAGGAGTTTCGGAAAGATTCGAAGAGGCCCTGAAACTTCTCGAGAGACTCGGTGCAAAGGTG GAGAGAGTGAAGATACCCCACATCAAGTACTCTGTTGCCACCTACTACGTCATCGCTCCAGCCGAGGCGA GTTCAAACCTTGCCAGATTCGACGGTGTGAAGTACGGCCTCAGGATAAAAGAGAAAGGCCTCAGGGAGAT GTACATGAAGACAAGAAACGTGGGCTTCGGTGAGGAAGTGAGAAGAAGGATCATGATCGGAACCTTCACC CTCAGCGCTGCCTACTACGAAGCCTATTTCAACAAGGCCATGAAAGTGAGGAGAAAGATCTCCGATGAGC TCAACGAGGTGCTCTCGCAGTACGACGCGATCCTCACTCCCACCTCCCCGGTGACGGCCTTCAAGATCGG AGAGATAAAAGATCCTCTCACCTACTACCTCATGGACATCTTCACGATACCCGCAAACCTCGCGGGACTT CCCGCGATCAGTGTGCCGTTCGGGTTCTCAAACAATCTCCCCGTTGGTGTTCAGGTGATAGGCAGAAGGT TCGCGGACGGGAAAGTCTTCAGAATAGCGAGGGCCATAGAGAAGAACTCCCCATACAACGAAAACGGCAT GTTCCCGCTCCCGGAGGTGAAAGCATGA
glutamyl tRNA-Gln amidotransferase, subunit B
locus tag = TM_1273 length = 1449 bp
>gb|AE000512.1|:1298742-1300190 Thermotoga maritima MSB8, complete genome ATGAGATACAGACCCGTCATAGGTCTTGAGATACACGTTCAGCTTTCAACGAAGACAAAAGCGTTCTGCT CCTGCCCGGCGGATGTCTTCGAGCTTCCTCCGAACACGGCGATCTGTCCCGTCTGCACCGGCCAGCCGGG GGCCCTTCCCGTTCCCAACGAAGAGATGATCAGGTTCGCCGTGAAGACCGCCCTTGCGTTGAACTGCAAG ATTCACAAGTATTCCAGATTCGACAGAAAGAACTACTTCTATCCTGACCTTCCCAAGGGATACCAGATCA GCCAGTACTTCTACCCGATCGCAACCGAGGGTTTCCTGGAGATAGACGGAGACGAAGGAAGGAAAAAAGT CAGAATCAGAAGGCTCCACCTCGAGGAAGACGCTGGGAAACTCGTCCACGAAGGGGACTCCATCACCCGT GCGAGCTACTCTCTGGTCGATATGAACAGATGCGGTGTTCCTCTCATCGAGATCGTCACAGAGCCCGATA TTTCTTCTCCGAGAGAAGCTCGCGTTTTCATGGAGAAGCTGAGATCCATCGTGAGGTACCTCGGCGTGAG CACGGGAGACATGGAAAAGGGAGCGCTCAGATGCGACGCGAACATCTCCGTTGTTGACACAGAAACGGGA AGACAGAGCAACAGGGTGGAAGTGAAGAACATGAACTCTTTCAGGTTCGTCGAAAGGGCTTTGGAGTACG AGTTCGAGCGAATTGTGAAGGCGATGGAGAGAGGAGAAGACGTGGAAAGAGAGACGAGAGGCTGGGACAT GGCCACGAAGATCACCGTTTCCATGAGAGGAAAAGAAGAAGAGAGCGACTACAGGTACTTCCCGGAGCCG GACATACCGCCCGTTGTTCTCTCCGACGAGTATCTCGAAGAGGTGAAAAAGGAACTCCCAGAGCTTCCAG ACGAGAAGGCAGAGCGATTCATGAGAGAGTACGGCCTTCCGGAGTACGACGCGAAGGTGCTCACCTCGAG TAAAGAACTCGCTGAGTTCTTCGAAGAGTGTGTGAAGGTGGTGAACAGACCGAAGGATCTCAGCAACTGG ATCATGACGGAAGTTCTGAGAGAACTCAACGAAAGAAACATCGAAATCACAGAGTCAAAACTGACCCCTC AGCACTTCGCTGATCTCTTCAAATTGATGGACGAAGGAAAAATCTCCATAAAGATCGCAAAGGAGATATT CCCGGAAGTCTTCGAAACAGGAAAGATGCCCTCCCAGATCGTGGAGGAAAAGGGTTTGACTCAGATCAAC GACGAAAAACTCATCGAGGAACTGGTGAAAAAGGCGATGGAACAGAATCCAAAGGCCGTTCAGGATTACA AATCAGGCAAAAAGAAAGCGGCAGGGTTTTTCGTGGGATACGTGATGAGAGAGACAAAAGGAAAAGCGAA TCCGGAACTCACGAACAGGATCATTCAAAAACTCCTCGAGGGGGAGTGA
glutamyl tRNA-Gln amidotransferase, subunit C
locus tag = TM_1273 length = 291 bp
>gb|AE000512.1|:264194-264484 Thermotoga maritima MSB8, complete genome ATGATAAAGGTTACAAAGGACCTAGTGCTTCATCTTGAAAATCTGGCAAGGTTAGAACTCTCCGAAGACC AGAGAGAAAGTCTGATGAAAGATTTTCAGGAGATACTCGATTACGTGGAGCTCCTCAACGAAGTCGACGT GGAGGGTGTGGAGCCAATGTACACACCCGTTGAGGACAGCGCCAAACTCAGAAAAGGAGATCCGAGGTTC TTTGAAATGCGGGACCTCATAAAGAAGAACTTCCCGGAAGAAAAAGACGGTCACATAAAAGTCCCCGGAA TCCACAGATGA
- Note that prolyl-tRNA aa synthetase is backward.
- Subunit A and Subunit B of the Gln amidotransferase are neighbors. There is a 4bp overlap between two genes. How this overlap is transcribed is unknown. I tried submitting the sequences to BLAST to find the actual sequence of the whole enzyme, but I did not retrieve any useful information.
Construction Files
Prolyl-tRNA aa synthetase
PCR ProRS-F/EcoRI-SOE-R on pcrpdt (485 bp, SOE_A) PCR EcoRI-SOE-F/ProRS-R on pcrpdt (1296bp, SOE_B) PCR ProRS-F/ProRS-R on A+B (1755 bp, newpcrpdt) Digest newpcrpdt (NcoI/EcoRI, 1739+10+6, L, pcrdig) Digest pBAD-myc-his-A (NcoI/EcoRI, 4053+41, L, vectdig) Ligate pcrdig and vectdig (pBAD-ProRS) ----------------------------------------- >ProRS-Fwd Forward primer for PCR of ProRS ccagtCCATGGcacggatgaaagacctctatgc >ProRS-Rv Reverse primer for PCR of ProRS ccagtGAATTCtcacctgacagcctccttggg >EcoRI-SOE-F Forward primer for SOEing PCR of ProRS CAGGGCGAGAGAGTTCATCATGAAGG >EcoRI-SOE-R Reverse primer for SOEing PCR of ProRS CCTTCATGATGAACTCTCTCGCCCTG
Glutamyl-tRNA Gln amidotransferase
PCR Gln AB RS-F/EcoRI-SOE-R on pcrpdt (205 bp, A) PCR EcoRI-SOE-F/Gln AB RS-R on pcrpdt (2718bp, B) PCR Gln AB RS-F/Gln AB RS-R on A+B (2894 bp, newpcrpdt) Digest newpcrpdt (NcoI/EcoRI, 2878+10+6, L, pcrdig) Digest pBAD-myc-his-A (NcoI/EcoRI, 4053+41, L, vectdig) Ligate pcrdig and vectdig (pBAD-Gln AB) ----------------------------------------- >Gln AB RS-Fwd Forward primer for PCR of Gln AB RS ccagtCCATGGcaatcgatttggacttcagaaaac >Gln AB RS-Rv Reverse primer for PCR of Gln AB RS ccagtGAATTCtcactccccctcgaggag >EcoRI-SOE-F Forward primer for SOEing PCR of Gln AB RS gaaaattctgggGAATcCcagttgcgatc >EcoRI-SOE-R Reverse primer for SOEing PCR of Gln AB RS gatcgcaactgGgATTCcccagaattttc
Glutamyl-tRNA Gln amidotransferase
PCR GlnC-F/GlnC-R on Thermotoga Maritima genome (312 bp, pcrpdt) Digest pcrpdt (NcoI/EcoRI, 300+10+6, L, pcrdig) Digest pBAD-myc-his-A (NcoI/EcoRI, 4053+41, L, vectdig) Ligate pcrdig and vectdig (pBAD-Gln C) ----------------------------------------- >GlnC-F Forward primer for PCR of Gln C RS ccagtCCATGGcaataaaggttacaaaggacctagtgc >GlnC-R Reverse primer for PCR of Gln C RS ccagtGAATTCtcatctgtggattccgggg
May 1st and Result
This was the last lab period for the semester.
I did mini-prep purification procedure to extract the plasmid DNA from the cells. The DNA was then submitted to DNA sequencing.
- Miniprep purification of DNA
MINIPREP (2mL) Procedure for Plasmid DNA Purification 1. Pellet 2 mL saturated culture by spinning full speed, 30 seconds in a 2mL Microcentrifuge tube. 2. Dump supernatant 3. Add 250uL of P1 buffer into each tube. Resuspend the cells thoroughly 4. Add 250uL of P2 buffer (a base that denatures everything and causes cells to lyse). Gently mix up and down. Solution should become clearer. 5. Add 350uL of N3 buffer (an acid of pH ~5 that causes cell junk - including protein and chromosomal DNA - to precipitate, and leaves plasmids and other small molecules in solution). Slowly invert a few times, then shake. 6. Spin in centrifuge at top speed for 5 minutes. 7. Label blue columns with an alcohol-resistant lab pen. 8. Pour liquid into columns, and place the columns into the centrifuge. Spin at full speed for 15 seconds. 9. Dump liquid out of the collectors under the columns (the DNA should be stuck to the white resin) 10. Wash each column with 500 uL of PB buffer. 11. Spin in centrifuge at full speed for 15 seconds, then flick out the liquid again. 12. Wash with 750uL of PE buffer (washes the salts off the resins). 13. Spin in centrifuge at full speed for 15 seconds and flick out liquid again. 14. Spin in centrifuge at full speed for 90 sec to dry off all water and ethanol. 15. Label new Microcentrifuge tubes and put columns in them. 16. Elute them by adding 50uL of water down the middle of the column (don't let it stick to the sides). 17. Spin in centrifuge at top speed for 30 seconds. 18. Take out columns and cap the tubes.
In this way, I prepared DNA extractions of prolyl-tRNA synthetase and glutamyl tRNA-Gln amidotransferase subunit C, which I then submitted to sequencing.
On May 2nd, the sequencing results came back.
- Pro RS Sample Sequencing Result
- Gln ADT C Sample Sequencing Result
Both results indicate that cloning and transformation of Pro RS and Gln ADT C were successful. The sequences are almost identical to its construction files.
April 29th and 30th
The ligated plasmids from previous week were transformed into the host cell using heat-shock transformation.
- Transformation by heat-shock procedure
1. Thaw a 200 uL aliquot of cells on ice 2. Add 50 uL of water to the cells (if greater volume is desired) 3. Add 30 uL of KCM to the cells 4. Put your ligation mixture on ice, let cool a minute or two (for Miniprep product, dilute by 10, then use 1uL of dilution) 5. Add 70 uL of the cell cocktail to the ligation, stir to mix 6. Let sit on ice for 10 min 7. Heat shock for 90 seconds at 42 (longer incubation may work better) 8. Put back on ice for 1 min 9. Add 100uL of 2YT, let shake in the 37 degree incubator for 1 hour 10. Plate 70+ uL on selective antibiotics, let incubate at 37 degrees overnight
The lab did not have enough plates for the entire class, so I divided a single plate into two parts, each labeled "Pro" for ProRS, and "GlnC" for GlnADT C transformation.
The next day, I observed that there were many colonies on both sides of the plate, indicating that transformation of either original Pbad plasmid or our ligated plasmid had occurred.
I picked out 2 colonies for each part (2 for ProRS, and 2 for GlnADT C), and incubated in 4ml LB media overnight, for the mini-prep purification. The rest of the plate was stored in the refrigerator.
April 24th
The vectors (from Karim) were ligated today, following the procedure:
- Ligation procedure:
1. Set up the following reaction: 6.5uL ddH2O 1uL T4 DNA Ligase Buffer 1uL Vector digest 1uL Insert digest 0.5uL T4 DNA Ligase 2. Pound upside down on the bench to mix 3. Give it a quick spin to send it back to the bottom of the tube 4. Incubate on the benchtop for 30min 5. Put on ice and proceed to the transformation
The two ligated products were then labeled after the inserts, ProRS and GlnADT C, and were stored in ther freezer.
April 22nd
No fruitful experiment could be done, as all the gel trays were taken by other groups. I tried digesting my Pbad vector, except I needed the gel purifying step for this, and without the trays, nothing could be done. I decided to use Karim's digested Pbad vector for ligation step.
April 17th
I gel-purified the digestion products from 4/10. I first ran a agarose gel, then cut out the band which were in their approximate location. For a higher resolution, I rant the gel for longer period of time.
April 15th
Purified DNAs from 04/10 were digested with NcoI and EcoRI. (as with our vector)
- Digestion procedure(30ul reaction mixture):
1. Set up the following reaction: 24uL of eluted PCR product 3uL of NEB Buffer 2 1.5uL NcoI 1.5uL EcoRI 2. Incubate at 37 degrees on the thermocycler for 1hr 3. Run an agarose gel, and melt with 600uL ADB buffer at 55 degrees. ****NOTE: If you are running short of time, this is an acceptable stopping point 4. If the DNA is shorter than 300bp, add 250uL of isopropanol and mix prior to loading it on the column
As step 3 says, since I did not have enough time to purify the DNAs, I stopped at mixing the products with ADB, and then stored the products in the freezer
April 10th
I examined my last attempt to SOE the pieces.
ProRS seemed to have been made judging by the size of the band.
GlnAB still failed. At this point I decided to stop working on GlnAB, and there could be some possible errors associated with this.
The first lane is the ProRS, and the next two lanes are their subparts. We see that the two parts have nicely annealed onto a whole product.
The 5th lane is where GlnAB should have been. Yet I do not see any visible trace of the product.
April 8th
April 3rd
I retrieved the PCR products from the freezer. I run an agarose gel to perform gel-purification step. I saved the GlnADT C for digestion step later. For ProRS SOE_A and ProRS SOE_B, the bands were cut and dissolved in a same vial in 650 ml ADB buffer. Similarly,Gln AB SOE_A and Gln AB SOE_B bands were cut and dissolved in a same vial in 650 ml ADB buffer.
These products (except GlnC) were then Zymo-cleaned, each dissolved in 12ul H2O.
So far, I have 12ul solution of ProRS SOE A+B, GlnAB SOE A+B, and 30ul solution of GlnC.
I set up the SOEing reaction, but with DMSO.
The 1st lane is ProRS subparts, and the 2nd lane is Gln AB subparts.
April 1st, Restarting
I restarted the experiment from the scratch again. This time, I replaced all the PCR step with DMSO version for better accuracy.
Following reaction mixture was made, and then put into a cycler.
20.7uL ddH2O 3.3ul DMSO 3.3uL 10x Expand Buffer (labeled 2) 3.3uL dNTPs (2mM in each) 1uL Oligo 1, 10uM 1uL Oligo 2, 10uM 0.5 uL Template DNA (Thermotoga maritima genomic DNA) 0.5uL Expand Polymerase (labeled 1)
Instead of 2K55 and 4K55 cycles, I chose 2K45 and 4K45 this time.
March 13-20th
The SOEing from 03/11 was a messy product. SOE would not anneal into a single product. GlnAB subunits especially demonstrated a large product that seemed to be larger than 4k bp. Over this period, I tried to fix the problem in one way or another, say carrying the SOE reaction in DMSO etc. After some analysis, I concluded that at some point, oligos were switched and the subparts (SOE) of the SOEing were also probably wrong.
March 11th
I gel-purified the PCR products from 03/06, and proceeded to SOEing reaction for ProRS and GlnADT AB.
- Splicing by Overlap Extention procedure:
1. Set up PCR reactions according to your construction file as normal 33uL reactions as described in Cloning by PCR 2. For each PCR, load 6uL of PCR product premixed with 4uL of loading buffer in a single well of a 1% agarose gel 3. Cut out the bands, put them into a single 1.5mL microcentrifuge tube 4. Add 650uL of ADB Buffer 5. Proceed with the Zymo Gel Purification procedure 6. Elute the DNA in 50uL of water 7. Set up your second round of PCR as a normal 33uL reaction using the eluted mixture of fragments as template
March 6th
First set of PCR reactions were set up. Following reaction mixture was made, and then put into a cycler.
24uL ddH2O 3.3uL 10x Expand Buffer (labeled 2) 3.3uL dNTPs (2mM in each) 1uL Oligo 1, 10uM 1uL Oligo 2, 10uM 0.5 uL Template DNA (Thermotoga maritima genomic DNA) 0.5uL Expand Polymerase (labeled 1)
So 3 sets of reactions were carried.
1) Prolyl-tRNA synthetase (ProRS)
2) Gln amidotransferase subunit AB (GlnAB)
3) Gln amidotransferase subunit C (GlnC)
March 4th
Genomic DNA and Oligos arrived. The oligos came in following amounts.
1. Pro RS Fwd: 32.4 nmol
2. Pro RS Rv: 31.0 nmol
3. Pro SOE Fwd: 25.1 nmol
4. Pro SOE Rv: 31.5 nmol
5. Gln subunit A+B Fwd: 36.0 nmol
6. Gln subunit A+B Rv: 29.1 nmol
7. Gln subunit A+B SOE Fwd 34.8 nmol
8. Gln subunit A+B SOE RV: 37.0 nmol
9. Gln subunit C Fwd: 37.0 nmol
10. Gln subunit C Rv: 29.1 nmol
These oligoes were diluted to 10 uM solution by first diluting the oligos into 100uM mixture (for example, for Pro RS Fwd, I diluted it in 324ul water), and then diluted 10-fold again.


text:
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Our first time using airbnb and first time in Ireland. Very pleasant overall! A few things we now know for next time: When catching a bus, figure out which side of the road one needs to wait. Ask the host to be specific. The 'freedom pass' for a few days around Dublin via bus is cost-effective & hassle-free. Not all rooms/areas pictured in an airbnb listing are used. This probably depends on the guest's & host's schedules. We didn't have time for anything except a little sleep and a quick bite. Aisling & her busy family were very accommodating for us rookies! Everything was greatly appreciated!Katrina2013-10-09T00:00:00Z
Ahhh such a lovely stay in Aisling's house. She dropped what she was doing to pick us up when we got off the bus at the wrong stop. She made us her, by now famous, breakfast daily which kept us strong and happy. She ordered us a cab when we were ready to depart - and we got a good hug upon departure - who could ask for more?David2014-10-07T00:00:00Z
Absolutely gorgeous family - made us feel right at home straight away. Only a short bus ride from the city but far enough that you can come 'home' and relax. Great breakfast each morning too and let us hang around waiting for our late flight. Will definitely be staying again 10/10Nicholas2014-11-08T00:00:00Z
It's really a cozy, wonderful place there, and we had great time sharing the our own experience. Aisling and her husband,John, did help me a lot. she's patient,passionate and eloquent. I believe she and her daughters must be good teachers. It's great to have a special experience here in Ireland. Hope to be there soon!!HSU EN2015-07-09T00:00:00Z
Staying with Aisling and John was a terrific introduction to my trip to Ireland, and encapsulated the heart of the Irish people - warm, hospitable, laid back and generous. Conveniently located in between the airport and the city (easily accessible on the bus route), all the facilities you needed for a great stay were provided. Aisling's incredible breakfast offering powered you for a good day's worth of exploration through the streets of Dublin. I'll call again when I next visit!Josh2015-05-03T00:00:00Z
Aisling and John were very gracious and accommodating hosts. The room was comfortable the breakfast was delicious. It was easy to find the place. From the ferry with the directions provided, and getting around with the train and bus was simple. Getting to the airport was 15 euros by taxi.Bernie Dee2015-10-30T00:00:00Z
I had such a lovely time with Aisling! She gave me help and support several time during the stay. (I know I must say thanks to her). It's a family home and you will have a comfortable and clean room. They are from Dublin so I had a chance to enjoy the Irish culture during funny chats with Aisling. The house is in a residential area and there is a near bus that connect to the city center in 20 minutes. She gave me the key and I was independent to leave or come back home when I prefered. Aisling and john are really really nice people, you're going to love them.Luca2014-05-12T00:00:00Z
A warm welcome and the absolutely most convenient stay for Dublin Airport!Anne2013-07-04T00:00:00Z
Room type
Private room
Property Type
Dublin, Dublin, Ireland
I was welcomed by Burak's cousin who was really welcoming and showed me where everything was and gave me the information I needed, from the buses to how to access their wifi. The neigbbourhood is lovely and quiet and very convenient as it's right next to the shopping centre plus 30 mins to city and 10 to airport! Pictures were identical to the house, so you won't be disappointed!Danielle2016-09-29T00:00:00Z
Clean and conveniently located. Recommended if you want to experience the north side of Dublin.Scarlett2016-09-12T00:00:00Z
I very much loved this place. It is right next to a bus stop that goes into Dublin and it is decently close to DUB airport. I never met Barak, but I met his cousin Eda, and she was very nice and always willing to help with anything.Sean2016-08-16T00:00:00Z
I had an amazing stay in Dublin, Ireland . Burak' cousin, Afa, is an adorable, thoughtful, kind, polish host. She helped me a lot. My bedroom was impeccable, I even had some presents (snacks, candys, an apple juice) put on the desk to welcome me. Concerning the morning, the kitchen is super large as well as window. The living room is very relaxing. I would like to thank Afa for pop corn present during my stay. Concerning the transport, it is really complicated for a foreigner despite the good bus traffic in the neighborhood. I would like to indicate that bus stops have numbers and not names, it doesn't exist bus map/plan at the bus stations/stops. I recommend to vistors a GPS (map downloaded before to come on the irish territory because my internet and cellphone network didn't work) and to look, before your arrived on (URL HIDDEN) website.Sebastien2016-10-28T00:00:00Z
This is an excellent location, by a big shopping centre and the bus stop to the city is just outside the house. Buses to and from the city centre after 7 pm are unreliable. I paid 15 Euro to the airport in a taxi, but was charged 21 Euro the other way. The room is good, very clean and modern with a small ensuite. There is everything you need. Excellent value.Rose2016-09-05T00:00:00Z
I liked my stay at Burak's place. I never met him, but communication with him was easy and prompt. Eda, his cousin, lives there and looks after the place. This is a large home with 6 separate bedrooms with private bathrooms. So it was nice to meet different travelers during my 4 night stay. The location is good. About a 10 to 15 Euro taxi ride from airport. You can get to/from the airport with city buses. It can take 30 to 60 minutes depending on the time if day and you need to walk a few minutes in between the different buses. There is a taxi stand around the corner. I arranged with a couple of other guests to share a taxi to the airport as it was raining and I didn't want to have to walk in the rain. There is a bus right in front of the house that goes into the city center. It takes about 30 minutes. Dublin city buses are double decker, so I always sat upstairs and enjoyed the scenery along the way. You can also take two city buses and visit the historical towns of Howth and Malahide. The Airbnb itself is very clean and well run. The breakfast items available were great and fresh. Towels and linens clean. Room was as shown. It is at the back of the house so no street noise. It is within a 5 minute walk to grocery stores. This is an Airbnb used mostly by people staying one or two days in between flights. So you will often hear people getting up and leaving early in the morning to get early flights. I liked this place and would stay again.K-Michele2016-08-22T00:00:00Z
The place is very clean and it is very convenient to travel as the bus stop is just right in front of the house. The surrounding is quiet and comfortable. It is a great place to stay for travellers.Wong2017-04-16T00:00:00Z
The welcoming was perfect & very kind & helpful.Ros2017-01-21T00:00:00Z
Room type
Private room
Property Type
Dublin, Dublin, Ireland
Amanda and Paul are amazing. They made everything possible to make our stay pleasant and easygoing. Every time we needed something, they were very helpful. The place itself is cosy and provide everything neccesary for a short stay in Dublin. We highly recommend to stay at their place.Veronika2015-10-17T00:00:00Z
We loved the City of Dublin! Amanda's place was perfect to relax after our long days! We would come back!Laura-Marie2017-05-18T00:00:00Z
Me and my boyfriend had fantastic time in this fantastic flat. We didn't have cold at all and the heating system was absolutely perfect. We cound't look for any better place in Dublin. The place is surrounded by green and is quite =) ! The only strange thing for us was the Dublin Bus Fares, quite axpansive.. to get to the flat from the city centre is 2,30 euro oneway each but in general Dublin and public services is really expansive compared to.. Italy ! I highly recommend this place.. thanks for everything !!!Giulia2012-03-21T00:00:00Z
The overall experience was good. There was a washer and stove you could use. There is no dryer, there is a drying rack though. The beds/covers are decent size. Small for a tall person. The shower could be better. It was quite difficult to enjoy a shower since the water mainly trickled out no mater how you moved the shower head. You can't control the overall heater to much but they provide smaller heaters to use. We also couldn't figure out how to turn the hot water on in the sinks. We had a problem with our toilet and they came right over to help us with it. Depending on where you are wanting to go the bus could be from 6-15 mins away from the house so you can walk. It can be expensive to take a taxi from the house to the city. Would recommend getting a leap pass while at the airport very helpful. It's about an hour bus ride or about 30 mins for taxi.Kat2016-10-19T00:00:00Z
Talking to Amanda before we arrived went well, no problems! Arrival was fine. Nice place to stay for a few days, it was nice having our own little flat. Wifi wasn't very good and a few things we had to learn while staying there. Over all, it was a good experience!Brittany2015-09-16T00:00:00Z
On both of the days I stayed in Dublin I left for the city center early in the morning and returned late so I never actually met Amanda but in talking online she has been very nice and the accomodations were great! Very nice apartment and definitely worth the priceDylan2015-11-10T00:00:00Z
A good place to stay and explore the city of Dublin.Henk2016-11-22T00:00:00Z
Amanda's place was perfect for my requirements - somewhere to stay whilst working in the area. Amanda and her partner were friendly and very helpful, I was well looked after. The Annexe is in a quiet spot and was very clean and well equipped. I would be happy to recommend the place and stay there again if I needed to!maureen2016-08-26T00:00:00Z
Room type
Entire home/apt
Property Type
Dublin, Ireland
First off Brian was very nice, he even picked us up from the DART because it was raining pretty good when we arrived. His place was the perfect way to end our trip; it was comfortable, had a great view and the breakfast stuff was so nice as well! Would love to come back and stay longer! Thank you againMandy2017-06-27T00:00:00Z
We loved staying in Brian's apartment and we really enjoyed our time in Howth. His home was in the perfect location; walking distance from the dart train and everything we wanted to see in Howth. He was so welcoming and went out of his way to make sure we felt at home. It was everything you could want from a host. The apartment was so clean and spacious and we were sorry that we weren't staying longer.Diana2017-06-26T00:00:00Z
Great location. Easy access to central Dublin, great sea coast walks, superb local restaurants. Brian's place works very well for two. Well equipped, very clean, very nice atmosphere and certainly bigger than we expected from the photos. Couldn't be happier.Perry2016-12-11T00:00:00Z
Fantastic stay, amazingly comfortable bed. Best location in Howth and so easy in and out of Dublin. The apartment offers a great Base and Brian has really thought about all of the little things long weekenders might need.Jessica 2016-10-25T00:00:00Z
Perfect place: hooked on Howth and this house feels like home ❤Marieke2016-10-19T00:00:00Z
It was a wonderful stay and in such a beautiful location! Brain was so helpful and available nearly 24/7 to answer any questions or help give directions on how to get from place to place. We will definitely love to stay again!sarah2016-07-11T00:00:00Z
Brian's apartment is lovely, spacious and most comfortable. Its position near the harbour allows proximity to water views and walks, excellent restaurants and the railway station (which makes it easy to commute into Dublin city). Brian's communication before and during our stay was first rate, and he gave us excellent suggestions for local places to eat which also included entertainment of Irish dancing and singing. He provided us with really nice food and coffee etc. and washing and ironing facilities and helped made our stay in Howth most enjoyable. Highly recommended.Warren2016-07-02T00:00:00Z
This is a fantastic place to stay. Aside from how cool the place is inside, the location is central to everything in Howth and close to public transportation. Brian is exactly what you except in a host and then some. He provided great recommendation for what to see in the area and where to eat, especially catering to the fact that we had a little one with us. We had too short of a stay, wish we could have stayed longer. Howth has so much to see (castles, hiking, lighthouses) and would be a great place to stay to explore the city of Dublin as well as being very close to the airport. Stay here, the place is great and Brian is awesome!Jennifer2016-08-15T00:00:00Z
Room type
Entire home/apt
Property Type
Howth, Ireland
A lovely room in a modern, beautiful house. We received a warm welcome with coffee/tea and cake in the garden on a sunny Sunday afternoon.Roger2016-10-10T00:00:00Z
Klaudia and her husband are lovely. Klaudia has assisted us to make our stay easy and enjoyable. The center of Dublin is close with the metro. Merci pour ces échanges lors du petit déjeuner.BERNARD2016-09-12T00:00:00Z
Great and clean place according with description located in a safe neighborhood, near to the airport (15min by car) but not so close to city center. For us it was not a problem because we had a car but I think that a fast way to reach city center is by train, 5 min far away from the place there is a train station. Klaudia and her family were great hosts and for sure we will stay again here if we return to Dublin.alex2017-04-25T00:00:00Z
Klaudia is a super host. Her house is very clean and the loft space very comfortable. The location is very close to the airport and a twenty minute drive to the port. We took a short drive to Howth where you can find the most amazing fish restaurants.Kevin2017-05-14T00:00:00Z
Lovely family! Very friendly and helpful. Treated us like close friends. Close to the train and other wonderful towns to explore. We hope to come back!Kari2017-07-31T00:00:00Z
Klaudia and her family were gentle, friendly, nice and very interesting. We spent our time so good thanks to them. Their house is well-situated: you can go from/to airport by taxi in 15 min and by Dart train to the city centre in 20 min. The house is in a quiet zone. When we arrived Klaudia gave us all the information about the city and places we need to visit. 100% recommended.Marina2017-02-16T00:00:00Z
The place was very nice and quaint. The shower was great! Grace and Laura were there to greet us and they were nice enough to let check in early as our flight got in at 9am. The house is very close to the train, making it easy to get into the city center. We met Klaudia and her husband just before we left and they all offered helpful information. We would definitely recommend staying here to anyone in Dublin!Marriah2017-04-14T00:00:00Z
Lovely room, very close to the airport and Malahide, super comfy bedsPhoebe2017-09-28T00:00:00Z
Room type
Private room
Property Type
Dublin, Dublin, Ireland
Great and affordableSara2017-05-10T00:00:00Z
Very friendly host! Allowed us to leave your luggage earlier than the check-in time. Always responsive pretty instantly at well. Definitely recommend!Tori2017-06-30T00:00:00Z
First of all, we didn't meet Burak but only her cousin. Although Burak's cousin was polite and helpful the room was without linens and bath towels because the washing machine had a problem and it was out of order. The first day, cousin of Burak told us that we will have towels the next day. We asked her one towel because we hadn't brought ours and we wanted to have a shower of course( in airbnb it was as an offer and we didn't get any information that the washing machine was off ). She gave us ONE towel but it was dirty. Moreover, there were spiders on the ceiling in the bedroom and in the bathroom. The mattress of the bed was a little bit noisy. The location was ok. Unfortunately, we weren't satisfied with our accommodation especially because they knew our reservation more than a month and I think that they should be more organised.Stelios2017-05-28T00:00:00Z
Good place if you have to go to the airport the next morning.marie2017-04-02T00:00:00Z
Close to the airport for an early flight out. Nothing too fancy but everything was clean and as described. It can get a little loud with guests coming/going in the middle of the night.Joanna2017-04-19T00:00:00Z
It was a chilly room, but the warm bed made up for it. The private bathroom and sweet breakfast contributed to a nice stay. I must admit I was surprised to learn how many rooms were available at Burak's home.Ali2017-03-23T00:00:00Z
We booked the accommodation for one night since it is close to the airport. We never met Burak in person. His cousin checked us in after some troubles with the doorbell. Finally we managed to check in and the room was as described. It is HIGHLY recommended to wear flipflops or house-shoes inside otherwise you will wipe the dirty floor clean with your socks... Since there were five other guests in the house, it was kind of noisy (especially one guest checked in after 11pm and woke us up). If you don't mind hostel feeling this is a good and cheap place near the airport. Mattress is old and uncomfortable. Positive is the shopping center next door, the bus stop in front of the house and the free parking spot on site. Won't stay again there.Peter2016-11-01T00:00:00Z
We really only used this as a place to crash before leaving to the airport early in the morning. This is definitely a business vibe airbnb with lots of other guests. There were a few things such as how we received our keys which left us feeling a little uneasy at first but all in all we felt safe, and we're grateful for an affordable option before our long flight home. We never met the host, but the communication was quick and efficient.Andrew2017-05-15T00:00:00Z
Room type
Private room
Property Type
Dublin, Dublin, Ireland
Donna's place is spotlessly clean and very spacious. We would definitely come back when we visit Dublin again. She was also very responsive and accommodative to our questions and requests - we arrived late at night and had no problem finding the listing and getting settled in. We felt at home. Thank you.Sanghyun2015-12-20T00:00:00Z
Donna was so friendly and helpful. She recommended a nearby pub where we had a fantastic meal and met even more wonderful people! Our rooms were spacious and tidy. It was a quiet location that was easily located. Thanks, Donna!Cynthia2016-06-10T00:00:00Z
Donna and her family were very welcoming and accommodating! We just needed a place to lay our heads before leaving for the airport the next morning, and this was perfect, since it's very near the airport. The beds were nice and comfortable! Thanks Donna!Anne2015-10-19T00:00:00Z
Donna was SO incredibly lovely, and so was her family. I don't think we could have asked for a better place to stay for our last stop before we headed back to the U.S. Both bedrooms were incredibly spacious and comfortable. She was even kind enough to let me hang out in the bedroom on our last day when I got sick at the end of our trip. It truly felt like family we were staying with, and we highly recommend her!Shelby2016-03-25T00:00:00Z
A perfect place for a short or long stay. Very comfortable and private.Jackie2015-08-20T00:00:00Z
So, if you don't have your own car and you can't afford a taxi everyday, this place can be your WORST NIGHTMARE ever. On top of it, if you're a girl travelling alone, please spare yourself the horror. I have traveled alone a lot and have taken a lot many airbnbs but this was my worst experience ever. I made a mistake of relying on some 200 reviews, most of which looked good. But what I didn't notice was how most of them complemented the parking space and the rest said that they just had to spend a night before taking a flight and it's good because it's close to the airport, which is true. So if you don't fall in either of the two sets of people, please conveniently avoid this place. This is going to be lengthy but it's important that you know this. So, I am going to be honest here, Donna otherwise seemed to be a sweet lady, but what I absolutely don't appreciate is the fact how the description of the property here doesn't reveal certain things correctly. I reached around 7.30 pm and was hungry for dinner when I was told by Donna that there are no restaurants close by but there's a 24/7 convenience store which is a 12 minutes walk from her house. Which is completely incorrect. The store is a 25 minute walk(even according to (SENSITIVE CONTENTS HIDDEN) maps) which normally wouldn't have been an issue but what Donna fails to mention here is that her house is in middle of nowhere. It's an extremely isolated and secluded area. She says it's safe and may be that's true, but you will not FEEL safe (again specially if you're a girl or even a bunch of girls travelling, sadly so). So, there are about 10-15 houses in a row, including hers and the walk till the closest bus stop (which is again about 25 minutes) and the one and only convenience store, is a walk along a path where you won't find a single person or a dog or any other being, or a house or any habitation WHATSOEVER and I am not exaggerating this at all. There are only bushes and endless fields behind those bushes on both the sides. So it's almost like a road in the middle of a jungle/ large field. And because of such a stretch, an otherwise almost half an hour walk seems to be endless. What's scarier is, at least I found it so, that some cars would pass you, which is scarier for a girl walking alone when the destination is almost half an hour away. I mean I'd like to be in an area where there's some habitation so that I can at least call for help, God forbid, something happens. So, it's the worst location ever if you don't have a car. Second, you're a girl alone, in a house middle of nowhere, with public conveyance 25 minutes away and the house doesn't support...Pragya2017-06-23T00:00:00Z
Donna was very friendly and helpful. Thank younadege2017-05-21T00:00:00Z
Convenient location to Dublin airport.Rose2017-08-19T00:00:00Z
Room type
Private room
Property Type
Swords, Dublin, Ireland
They room was great. Enough floor and desk space to hold my bags and belongings. The door between the room and the rest of the house can be locked if that's important to you, but they're totally trustworthy in my opinion. There are also a kettle/mug/selection of tea in the room for whenever you want. The mattress was comfortable and the blanket was warm. There is also a universal travel adapter plugged into the wall in case you forget to bring one, but be warned! It only adapts the plug shape, not the voltage or frequency so make sure your devices are compatible with 230 V 50 Hz outlets before using them. Breakfast was a pretty skookum omelette, very satisfying. The only thing I could possibly consider as a downside is that every 15 minutes or so you can hear a train drive by in the distance, but that is the price you pay for easy access to transport. It isn't even loud and never bothered me, but it is something to be aware of if you are a light sleeper. The trains stop after about midnight. Overall, an excellent place to stay with easy access to both Howth and Dublin.Duncan2017-09-16T00:00:00Z
Bernice's place is in a wonderful part of Dublin and close to the sea with beautiful sunsets and good Thai food and Guinness close by. Most importantly, the breakfast is just superb!Alan2017-08-31T00:00:00Z
This was my first Airbnb experience and it exceeded my expectations! Bernice & the family were so friendly, helpful and respectful of my space and odd dietary needs. The room was very clean, well equipped and comfortable, and the location ideal for easy access to Dublin and Howth. Highly recommended!Suzanne2017-06-11T00:00:00Z
Highly recommend staying here :)Jenni2017-07-18T00:00:00Z
Bernice was kind a super-host!!! Super nice! Well communication before arrive, gave all kind of info and help during my (short) stay... gave a hand when needed... she always tried to make my stay as confortable as possible... Thanks a Lot! Her place is very nice, ultra clean and comfy room with all that you´ll need and also very close to Sutton Station (8´min walking to... and 25´min DART ride to Dublin). Highly Recommended!!!Juantxo2017-08-24T00:00:00Z
Great Location, very friendly host ! Delicious irish breakfast, the room was very clean. I would come again !Gabi2017-07-25T00:00:00Z
Excellent says it all!Elma2017-08-23T00:00:00Z
Great room on a private street. Firm bed, clean bathroom and breakfast was awesome. Close to DART station which makes it super easy to catch the train into Dublin or a bus to the airport. Bernice has maps and guides to local pubs and sites, and made good recommendations. Great host.marr2017-09-22T00:00:00Z
Room type
Private room
Property Type
Sutton, Dublin 13, Ireland
Eileen and her husband were wonderful hosts! The room is small, quiet, clean, and very comfortable. Their home is a very short walk away from the public transportation which will get you into Dublin in only a few minutes. Eileen provided maps, great sightseeing advice, and even walked me to the train to make sure that I knew where I was going.Lauren2017-07-10T00:00:00Z
Small room but just as described. Parking space by house. Breakfast was very good with homemade bread. To travel into the city takes about 20 min by train from Bayside to Tara station. 3 day ticket costs about 17 EUR. Eileen was a fab host and delivered all information needed. I really liked our talks and her hospitality.Britta2017-03-19T00:00:00Z
I had a great stay. Eileen was nice and helpful. The room was not big, but it had a private toilet and a shower. The location is good, within walking distance of a bus stop and a train station. There is also a pizzeria nearby. Every morning, Eileen served a breakfast: bread, toasts, tea, coffee, juice, milk and such. When I was departing, she drove me to a bus stop. There were some minor problems. For example, their water system was making noise whenever anyone in the house was using water. Nevertheless, I would highly recommend Eileen's house, and I'd like to encounter hosts like her more often.Evgeny2017-08-16T00:00:00Z
Great position to visit Howth and Malahide. There is also a bus to the airport from there only €3.30 ... saves going back into the city. Eileen is very helpful and friendly and the room is clean n comfortable with ensuite.Cath2017-07-03T00:00:00Z
This is the right place for those who are used to commute every day at least 30 minutes with public transport in big cities. For the people, like me, not too into night life, it's the best place to be. Public train (DART) just at 3-4 minutes walking, and 20 minutes from Dublin Center. Two stops only from Howth with its incredible cliff walking paths and glorious fish and seafood restaurants.BARTOLOMEO2017-06-15T00:00:00Z
Clean and comfortable accommodation within easy reach of either the city centre or the beautiful northern Dublin coastal areas. Great value for money (plus a continental breakfast included) with extremely friendly and convivial hosts. Irish hospitality at its best .Nigel2017-08-06T00:00:00Z
Great hosts! Friendly and helpful. Eileen's bread is the highlight of my dublin trip!Eddie2016-11-13T00:00:00Z
Exactly as described. Eileen's a helpful and friendly host. Quiet, residential part of the city. Easy to get to the centre on the DART. All good.David2017-07-13T00:00:00Z
Room type
Private room
Property Type
Sutton, County Dublin, Ireland
Very nice ensuite room with own entrance. The host is the nicest lady. The location is very close to Dublin Airport.Monique2017-09-16T00:00:00Z
Arrived late and Lorraine had the room warm and the takeaway menus ready. Comfortable and clean room. Would recommend.Ken2017-09-14T00:00:00Z
Lorraine is a lovely helpful host and the room is very tastefully put together. I thought the choice of snacks and drink in the room was a nice touch. Would highly recommend a stay.arif2017-09-13T00:00:00Z
A cosy and quiet room with private entrance and bathroom. The city can be reached easily by bus or train. Lorraine and her husband were very helpful and friendly. I had a short and busy stay but I'd gladly visit again!Jænne2017-09-24T00:00:00Z
It is a really nice place to stay. Hosts are great and really helpful. City center is easy to go with a bus 1 minute away from the place. I recommendThomas2017-09-17T00:00:00Z
Perfect location for me! Great public transport to the city if you need. Great, quiet and everything you need. Nice to have on suite, and facilities to eat and drink without disturbing the host! Lorraine couldn't be nicer and more helpfulMatthew2017-09-21T00:00:00Z
Room type
Private room
Property Type
Dublin 13, County Dublin, Ireland
Howth is lovely. Great food. Cliff walk. Scenic view. Pier activity. You can't go wrong.Maggie2017-09-04T00:00:00Z
It is a good place to stay because it is near the harbour and the village!Cassandra2017-09-11T00:00:00Z
The room is realy good. The only thing I do not feel satisfied is that I did not have breakfast which is on the list.ZHEHAN2017-09-05T00:00:00Z
Gorgeous view from Daragh's home. He will give you precious suggestions about where you can eat tasty sea food. At Daragh's pub, you can drink great beers and special alcoholic drinks. Really recommended.Evaristo2017-07-16T00:00:00Z
Daragh's helpful and his place is quite yet also close to the most characteristic place of the city: the harbour and its sealsDavide2017-08-22T00:00:00Z
Good place to crash for the night . Daragh place was a bit hard to find . Very clean and privateCheryl2017-08-27T00:00:00Z
Good location near the harbor and things to do (beautiful view, too). Small room, but clean and has a private bathroom. Daragh was flexible with our check in time which was especially nice.Megan2017-09-25T00:00:00Z
Nice simple room with ensuite shower. Perfect location just 5 mins from East pier and great pub, The Harbour Way 5 mins away with music on.Kevin2017-07-24T00:00:00Z
Room type
Private room
Property Type
Howth, County Dublin, Ireland
My room asnd private bath were clean comfy and warm. I had plenty if space and all needs met for my 10 days. Bernice made amazing breakfasts and offered helpful tips. it eas only a few minute waslk along a paved pathway to the dart or bus or to the start if a lovely coast beachwalk...sutton has good food and a pretty big market too. I would highly recommend staying and getting to know sutton howth area and your lovely host!Annastasia2017-09-29T00:00:00Z
Really nice place to stay, close to a really nice restaurant and to the beach. We had a good time here and loads of information from our host. We highly recommend it. Fabienne and Philippe from FranceFabienne2017-08-08T00:00:00Z
We had just arrived in Dublin and were fairly exhausted from our travels. We absolutely enjoyed our stay here. It felt so good to have a mother in Ireland! :) The hearty traditional Irish Breakfast kept us going all day. The room was very clean and cozy, with our own private bathroom. Our hostess was kind enough to share all sorts of recommendations for a successful journey across Ireland. Would definitely stay here again!Brandon2017-09-08T00:00:00Z
Highly recommendedJames2017-05-13T00:00:00Z
Lovely hosts, great location highly recommended.Jerome2017-04-25T00:00:00Z
We loved the location. Free parking for our car. Si close to the Dart, so easy to travel in/out of the city. centre. We would stay here again if visiting DublinJenny2017-07-16T00:00:00Z
Shauna is very friendly welcoming host. She was great at giving advice/directions. The room was very nice, comfortable bed, with tv and tea/coffee facilities and even a bottle of fresh water and glass by the bedside, lots of tourist brochures/leaflets to look at, a tv and my own bathroom. all the house was very clean and pleasing. Breakfast was lovely, full fry, orange juice and all lovely cooked. it was a pleasant stay. thanks shaunaSara2017-04-02T00:00:00Z
Beautiful place and right near the sea, amazing welcome.. stay here you won't regret it :)James2017-08-29T00:00:00Z
Room type
Private room
Property Type
Sutton, County Dublin, Ireland
Toni had a wonderful location on the waterfront. We would recommend to anyone. It's a quaint town with great food nearby and the cottage is wonderful! The pictures don't do it justice. It's more than we could have asked for.Meghan2017-09-05T00:00:00Z
Excellent location, Toni is a lovely host and the cabin is very modern. Beautiful view would stay again.Marcus2017-07-31T00:00:00Z
I stumbled across this advert and had to stay here as soon as I saw the pictures. The photos don't do this lovely cabin justice. It's newly built, well thought out, comfortable, clean, the fridge came stocked with breakfast and snacks, very nice strawberries were left out for us... Our favourite airbnb yet! Toni is a love - she tended to all our needs and thought of every detail, including gathering various menus from restaurants in town for us! Nice shower soaps were left for use. The linens smelled faintly of lemon. The grass is so soft and green... Town is a 10 min walk via beach or 15 min walk via road. Or you can sit in the chair and watch the tide roll in and out. We will be back for a longer stay. Stunning place, loving people!Jenna2017-06-21T00:00:00Z
We had a really nice stay in the Cabin. We're 3 people, but anyway I could sleep in the couch which it becomes a double bed and it was fantastic. The area is awesome, the cabin is in front of the sea and the scenery couldn't be better. It's a quiet zone but near to Dublin. They left food for in the first day and always were very kind with us. We had some problems the first day because of the car, and they helped us with everything. Thank you so much Toni, I hope see you soon. You're such a nice people.Judit2017-08-24T00:00:00Z
Toni is a superb host. Very welcoming and very happy to offer lifts into town and advice at any time. The cabin has amazing views over to Irelands eye and the mountains in Northern Island. After our trips to Dublin it was wonderful to walk back to the cabin along the beach from Howth station - the best commute for me ever! The cabin was extremely clean and brand new. The fridge was very well stocked for 2 great breakfasts and a beer for watching the sunset at the end of the day. Thank you Toni, we will be back:)Stuart2017-07-13T00:00:00Z
Gorgeous accommodations with beach access perfect for a getaway. Very kind and friendly hosts who were happy to offer suggestions or help figuring out transit. Easy train ride to Dublin but the beach walk to Howth can't be missed! A definite must-stay.nicole2017-07-16T00:00:00Z
The cabin is just as the photos show--charming, comfortable, and perfect for a getaway to Ireland. The cabin has everything you need to feel comfortable away from home. It's also a short walk to the town of Howth with wonderful restaurants and a train station that will take you to Dublin within 20 mins. I can't say enough how much I enjoyed my stay here. Toni ensured I had everything I needed during my stay and was very helpful at helping me navigate to where I wanted to go.morgan2017-07-10T00:00:00Z
This is the one, do not even think about booking anywhere else in Howth. This is the sweetest spot near Dublin, literally steps from the ocean. The entire cabin is newly constructed and outfitted with the best of everything. Perfect place to get away from hustle and bustle of Dublin. Wonderful hikes along massive cliffs, great restaurants, and Toni and Leanord are absolute top quality people. Please book here if you get the chance.Gunnar2017-08-26T00:00:00Z
Room type
Entire home/apt
Property Type
Howth, Co Dublin, Ireland
Mary is the perfect host, we really enjoyed our stay, loved every minute of it! Beautiful room, nice bathroom, the breakfast was delicious, and the house is close to the beach and to Dublin city. Everything you could wish for during your holliday!Monique2017-07-12T00:00:00Z
Although this was a short visit (just an afternoon and evening) it was one of our favorite stays on a 2 1/2 week visit to England & Ireland. Mary is a wonderful host and we thoroughly enjoyed our time chatting with her about life, music, dogs, & family. Her house is lovely - we had a floor to ourselves and she had a full breakfast ready for us before we headed to the airport. She recommended Howth for our evening dinner and it was one of the best recommendations of the whole trip. Would definitely stay there again.Rick2017-07-19T00:00:00Z
Everything was perfect! With navigation it was realy easy to find and we had a very warm welcome. The breakfast was even better than in some hotels! We loved it and would recommend to stay there any time.Stefanie2017-06-11T00:00:00Z
We very much enjoyed staying at Mary's place. It was a perfect choice for our trip to Dublin. We found the room cozy and well-equipped. We particularly appreciated the on-suite bathroom and the sense of privacy that came from being at the top of the house. Mary was a generous and warm host, who we enjoyed talking to. We would be happy to stay again at Mary's place if we were to visit Dublin again.Alíz2017-05-22T00:00:00Z
Mary's place is wonderful and Mary is a great host. The room is very private and quiet as it is located upstairs. The room is decoratly tastfully and Mary even put a little welcome chocalate on the pillow. Breakfast was delicious with coffee, tea, toast, jam, fresh berries and juice being beautifully presented. I would recommend Mary's place highly.Theresa2017-04-02T00:00:00Z
Mary was so very helpful!We Hand some Problems with the Motorway fee, she completed it in the Internet. We asked for a nice pub, she drove us there! Thank you very much! We will Come back!Rainer2017-05-28T00:00:00Z
Mary is by every standard the perfect host. Her home is immaculately kept and everything about our short stay was exceedingly comfortable and accommodating. We highly recommend staying with Mary if you are traveling to the area. Lovely neighborhood with a gorgeous walk by the sea just steps away! Perfect experience!!Colee2017-09-30T00:00:00Z
Staying with Mary was a wonderful experience. SHe was easy to communicate with and went far out of her way to make sure we were comfortable! Easy and close transport to the city center and right next to the beautiful coast.Douglas2017-08-14T00:00:00Z
Room type
Private room
Property Type
Kilbarrack, County Dublin, Ireland
good place for arriving late or departure early in the morning at the airport. they are very kind and the offer a pick up and drop off to th e airport for a little bit. recommend at all timesRaphael2017-06-15T00:00:00Z
Nice family, charming, warm welcome. Good communication, good value for price, near to airport. Recommended. Thank you very much!Oliver2017-08-09T00:00:00Z
It was awesome stay at Flore's house with her family. Flore give us a ride from and to airport, which was really helpfull. Flore and her husband were very kind, always ready to help us in every way we need and give us some practical and sightseeing hints. Thanks to them, our stay in Dublin gets another great experience - it was like being "home", not on vacation. I can only recommend to choose Flore, also the location is good - there is big supermarket, restaurants and bus stations nearby. It takes max. 30 minutes by bus to the Dublin center.Robert2017-07-31T00:00:00Z
Flore was a very gracious host. She picked us up from the airport, gave us perfect directions how to get into the city and back and even drove us back to the airport for our morning flight. I'd highly recommend Flore's place for us proximity to the airport.Tracy2017-07-23T00:00:00Z
Great place to stay. Close to the airport, but a bus stop is close by if you wish to go into the city centre. Flore has a lovely family & they were all very welcoming. Flexible check-in time too. Just remember - the address is Rd NOT Avenue. (SENSITIVE CONTENTS HIDDEN) maps from Airbnb directed us to Ave, even though it says Rd. (Flore is trying to get this corrected, so it may no longer be a problem.)Nancy2017-09-15T00:00:00Z
Lovely stay at Flores, her husband picked us up from airport late at night and Flore was very friendly & accomodating. House was spotless. Highly recommendAnn2017-08-06T00:00:00Z
Great place! Flore's family will receive you with arms wide open. The house is clean and of course well equipped. You won't be wrong here.MATIAS2017-09-03T00:00:00Z
Flore and her husband are super friendly, was very nice to converse with them. Very close to airport, public transport right around the corner. Nice quiet family neighborhood, clean room, clean bathroom.Jamal2017-09-17T00:00:00Z
Room type
Private room
Property Type
Belmayne, County Dublin, Ireland
Sandy was a wonderful host, and we loved our time at her place! Everything was perfect-- best Airbnb experience yet! We'd recommend going to the town of Howth and doing the cliff hike there; Sandy recommended it to us and we were not disappointed!Sophie2017-07-19T00:00:00Z
The place is secluded...she is not accommodateNERINA2017-06-10T00:00:00Z
Very nice room, clean and cosy and Sandy was very kind ! Be careful if you are tall though, ceiling is low :)Claire-Maha2017-08-26T00:00:00Z
It was a nice stay at Sandy's! We had a good time and she even dropped us off at a bus station, when we had to go back to the airport!Magdalena2017-09-17T00:00:00Z
It was a short but nice stay. we knew that the space wasn't big en high, so that was fine. There's everything you need in the room. The bed was a little small for us...Eefje2017-07-01T00:00:00Z
This was the best airbnb we have done. It was a perfect distance from the airport, and in between some amazing countryside and the city of Dublin. Sandy was everything you could ask for in a host. The room was very clean and very comfortable!Taylor2017-06-17T00:00:00Z
Very comfy! She was very friendly. Easy to talk to, which made us feel comfortable and welcomed. Room was spacious and clean. She provided us with some fruits, and teas. Would definitely recommend!Jose2017-06-14T00:00:00Z
Sandy is a wonderful host and her son is adorable!! Had the best sleep and definitely will be back again! Thanks againMaire Antoinette2017-06-22T00:00:00Z
Room type
Private room
Property Type
Sutton, County Dublin, Ireland
The space is very clean and caters specifically to AirBnb'ers. It's close to public transportation (just take bus 14 to Marlborough). Adrienne is very prompt in her responses and very accommodating. The only downside is that you have to share a bathroom. Other then that the place is spectacular! Would recommend and would absolutely stay there again.Pablo2017-08-16T00:00:00Z
Every room is labeled for different guests to use, it even has basic products if you forget them home. She was very helpful and called Airbnb to fix a problem with the bookingMarcos2017-09-27T00:00:00Z
Very clean and cozy!Andrea2017-08-20T00:00:00Z
Adrienne was a great host, providing lots of useful information in advance as well as after I arrived. The house and my room and bed were really nice and comfortable with all facilities well organised including labelling in the kitchen. The range of cereals was brilliant. Wifi in the house worked well. I liked the fact that Adrienne is a keen recycler and enabled that in the house. It really appreciated being in such a quiet house and neighbourhood too. Adrienne was friendly, helpful and responsive to requests and made my stay a very pleasant one(URL HIDDEN)Michael 2016-11-28T00:00:00Z
Great place to stay, every detail is more than taken care of and you can tell the host cares a lot..Jivesh2017-08-22T00:00:00Z
Decided to stay at Adrienne's place again for a reason. The location cannot get any better and the house is very well organised, very clean and Adrianne is putting a lot of effort to make the guest's stay the most comfortable and enjoyable. Would definitely stay again.Manmohan2017-03-22T00:00:00Z
We appreciated the detailed instructions prior to our arrival. The kitchen was well stocked with cooking equipment and condiments. The downside was noise from other guests and the wait to use the bathroom in the morning.Craig2016-09-13T00:00:00Z
Wonderful experience, Adrienne runs a great BnB and the whole thing went smoothly. Highly recommend staying here. Very clean and accommodating.Grace2017-06-06T00:00:00Z
Room type
Private room
Property Type
Dublin, Dublin, Ireland
Very nice room. Comfortable bed. Close to the airport. Private bathroom was a nice touch. I enjoyed meeting and spending some time talking to the family. Perfect for my needs.Dale2017-08-22T00:00:00Z
My best experience during my trip to dublin. Warm-welcoming ready to help me, they immediatly make me feel very secure when i was about to go out they even give me their phone number in case of trouble. They really take care about my comfort they also allow me to leave my bag before the room was avaible. They help me a lot to reach the first bus to dublin. The bathroom and the bed was clean and agreable, accompanied with luxuous towel i feel very comfortable. And finally my night was very good i feel isolated from the noise coming from inside or outside. I have good memories of my stay, they are the people you enjoy to met when you travel in a foreign country. Ma meilleure expérience durant mon voyage à Dublin. Ils sont extrêmement chaleureux très accueillant, prêt à aider, ils m'ont immédiatement fait sentir que j'étais en sécurité lorsque j'allais dans la capitale il m'ont donné leurs numéros en cas de problème. Ils étaient vraiment très attentifs à mon confort ils m'ont permis de laisser mes bagages avant l'emménagement pour que je puisse visiter la capitale ils m'ont également beaucoup aidée atteindre le premier bus pour aller à Dublin. La salle de bain et le lit étaient très propre accompagnés de serviette de toilette de luxe. C'est une famille extrêmement gentille ils font les choses correctement. Ma nuit de sommeil a été agréable je me suis sentie complètement isolé de tous les bruits venant de l'intérieur ou de l'extérieur de la maison. En tout cas je garde un bon souvenirs de mon séjour se sont des personnes que l'on adore rencontrer lorsque l'on voyage à l'étranger.Sia2017-09-17T00:00:00Z
A great place to stay and very clean. Siobhan and her family were friendly and helpful. 15 to 20 minute walk to a grocery store, an hour long walk to the nearest castle and a 10 minute walk to the nearest bus that takes you to the city. The room is also baby/ family friend. Would stay again.Veronica2017-08-31T00:00:00Z
Good communication, Jooe met us and made us feel welcome. Only stayed one night as it was near the airport and convenient for us as we left Ireland and went onto the next part of our tri. Joe helped us with directions as we were leaving early in the morning. Sorry we had trouble with the key in the morning. Hope it was ok.Brian2016-08-22T00:00:00Z
The room was very pleasant and everything seems to be very comfortable! Including the Bathroom with a great shower. Siobhan and Joe are lovely they made us our stay perfect and we had very good conversations!! If we ever traveling to Dublin their Room is definitely an adress to stay. Thank you both!Angelique2016-07-18T00:00:00Z
Siobhan and Joe were absolutely lovely and really laid back. The room was gorgeous and the neighbourhood was really nice! Train station about 20 min walk away through a really nice park and bus stop 10 min away. Would 100% recommend.Leah2016-09-04T00:00:00Z
Great night before we flew out of Dublin. Joe and Siobhan were very friendly and helpful. Very close to the airport so it was an easy morning to catch our flight. Great place for coming into or going out of Dublin.Rachel2016-10-31T00:00:00Z
beautiful apartment, VERY comfortable bed and pleasant hostНикита2017-05-07T00:00:00Z
Room type
Private room
Property Type
Dublin, Dublin, Ireland


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