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Peru's Interior Ministry has launched a radio program to help combat human trafficking. The program, called #QueNoTeEncuentren (So they don't find you) is aired in three languages – Spanish Quechua Full Article »
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T-Mobile G-Slate Android 3.1 Honeycomb Update Rolling Out Now!
T-Mobile has provided a dose of good news for T-Mobile G-Slate owners as the Android 3.1 Honeycomb update is rolling out now! Bringing with a host of enhancements the update will appear on your tablet when it is available for install. T-Mobile of course insists that representatives cannot "expedite" the delivery of the update or push the update to tablets by request." So what kind of new goodies will you find … [read full article]

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Embryonic development is a physical process during which masses of cells are sculpted into functional organs. The mechanical properties of tissues and the forces exerted on them serve as epigenetic regulators of morphogenesis. Understanding these mechanobiological effects in the embryo requires new experimental approaches. Here we focus on branching of the lung airways and bending of the heart tube to describe examples of mechanical and physical cues that guide cell fate decisions and organogenesis. We highlight recent technological advances to measure tissue elasticity and endogenous mechanical stresses in real time during organ development. We also discuss recent progress in manipulating forces in intact embryos.
Embryonic development follows instructions prescribed by the genetic material of the embryo and maternally-derived cues deposited in the egg prior to fertilization. Analysis of the spatial and temporal expression of genes has enabled developmental biologists to describe the choreographed motions of embryogenesis as gene regulatory networks – blueprints of the chemical signaling interactions required for tissue specification (Li and Davidson, 2009). However, development is also a physical process wherein tissues bend, fold, and twist into the elaborate structures of mature organs, and the mechanical stresses associated with these alterations have long been recognized as potentially conveying information to the developing tissues (Beloussov and Grabovsky, 2006; His, 1874; Mammoto and Ingber, 2010). Mechanical properties relevant to developing embryos include isometric tension (that is, contraction of the cytoskeleton), stiffness (elasticity of the cells and their surrounding substratum), pressure (stress perpendicular to a surface resulting from differences in fluid volume), and shear (stress parallel to a surface by friction from fluid flow). Here we review how mechanical stresses instruct organogenesis, and describe novel strategies to measure and manipulate these physical properties in the embryo.
Endogenous cytoskeletal tension and exogenous mechanical stresses regulate a large number of cellular behaviors, including cell turnover (proliferation and death) and various modes of differentiation. Tension transmitted from the extracellular matrix (ECM) through transmembrane integrin receptors to the cytoskeleton cooperates with cyclins to control transit through the G1 phase of the cell cycle (Assoian and Klein, 2008; Chen et al., 1997; Huang et al., 1998; Huang and Ingber, 2002; Klein et al., 2009; Mammoto et al., 2004). Endogenous mechanical stress is mediated in part by signaling through the small GTPase Rho, its effectors Rho kinase (ROCK) and mDia, and subsequent effects on myosin phosphorylation, actin polymerization, and actomyosin contraction. Tension-mediated increases in RhoA activity lead to ubiquitin-mediated degradation of the cyclin-dependent kinase inhibitor p27kip1, which then permits G1 phase progression (Mammoto et al., 2004). As described below, tension is thought to play a key role in regulating cell proliferation during development of the lung airway epithelium. Endogenous mechanical stresses also regulate programmed cell death. When the ECM scaffold is depleted or removed such that cells no longer have sufficient resistive adhesive contacts, the resulting decrease in cytoskeletal tension and cell rounding leads to apoptotic cell death (Chen et al., 1997). Such death is commonly seen concomitant with the loss of basement membrane during physiological involution of the mammary gland (Lund et al., 1996; Wicha et al., 1980) and tissue remodeling during amphibian metamorphosis (Mathew et al., 2010).
In addition to increases in cell number and localized apoptosis, embryonic development requires refinement of phenotypes as multipotent stem and progenitor cells differentiate down the various lineages that comprise the mature organism. Recent studies of mesenchymal stem cells (MSCs) have revealed a strict regulation of differentiation by the mechanical properties of the microenvironment and endogenous cytoskeletal tension (Engler et al., 2006; McBeath et al., 2004). Indeed, it appears that the differentiation of multipotent MSCs is tuned by the stiffness of the surrounding ECM – a soft microenvironment promotes neuronal differentiation, whereas a stiff microenvironment promotes osteogenic differentiation (Engler et al., 2006). Differentiated function is also regulated by cytoskeletal tension, as the rhythmic contractility of embryonic cardiomyocytes is optimal on ECM with mechanical properties matching that of the heart in vivo (Engler et al., 2008).
Differentiation is not always a terminal process, however. Epithelial-mesenchymal transition (EMT) is a phenotypic transformation by which epithelial cells detach from their neighbors and become motile and invasive. These phenotypic changes are driven by alterations in gene expression: downregulation of epithelial markers including E-cadherin and epithelial keratins, and upregulation of mesenchymal markers including N-cadherin, vimentin, and α-smooth muscle actin (αSMA) (Kalluri and Neilson, 2003). Embryonic EMTs are responsible for gastrulation and creation of the neural crest; pathological EMTs are thought to be involved in cancer progression (Thiery et al., 2009). Depending on the cell type and environmental conditions, EMT can be induced by a number of soluble stimuli, including cytokines and proteases. In transgenic mice, overexpression of MMP3 (also known as stromelysin-1) causes EMT, fibrosis, and eventual formation of frank tumors in the mammary gland (Sternlicht et al., 1999). In culture, treating mouse mammary epithelial cells with MMP3 leads to EMT through induced expression of Rac1b, a highly active splice variant of Rac1 (Radisky et al., 2005). Here, the EMT process is accompanied by striking changes in cellular morphology, specifically an increase in cell spreading against the underlying substratum. This increased cell spreading is necessary for MMP3-induced alterations in gene expression, as forced cell rounding prevents MMP3-induced EMT (Nelson et al., 2008b). These observations suggest that endogenous mechanical stresses may regulate EMT signaling. Indeed, in two-dimensional epithelial tissues in culture, TGFβ-mediated EMT is restricted to regions of high endogenous mechanical stress (Gomez et al., 2010). Elevated mechanical stress causes enhanced nuclear localization of the serum response factor (SRF) cofactor myocardin-related transcription factor (MRTF)-A, which itself regulates the transcription of a wide variety of genes associated with proliferation and actin cytoskeletal dynamics (Olson and Nordheim, 2010). As will be covered below, mechanical stresses accompany regions of EMT during heart valve formation, further suggesting that embryonic EMTs may be regulated by mechanical stress.
The adult human lung is comprised of a volume-filling tree of approximately 17 million branches that conduct air to 300 million alveolar gas-exchange units with a total surface area of 70 m2. Lung development proceeds via two sequential processes: branching morphogenesis of the epithelial tree during the pseudoglandular and canalicular stages (~5–24 weeks in human; embryonic days 12.5–17.5 in mouse), followed by development of the air sacs and alveoli during the saccular and alveolar stages (~24 weeks to postnatal in human; embryonic days 17.5-postnatal in mouse) (Hilfer, 1996). The development of the larger bronchial airways of the mouse has been mapped using immunofluorescence analysis of fixed specimens, and was found to be identical between individuals, suggesting that early branching of this organ is stereotyped (Metzger et al., 2008). The airway epithelium is separated from the lung mesenchyme by a basement membrane layer containing laminins and type IV collagen. Electron microscopy analysis (Bluemink et al., 1976) and immunostaining (Mollard and Dziadek, 1998; Moore et al., 2005) of embryonic lungs revealed that the basement membrane thins out adjacent to growing buds, suggesting that the ECM is either physically stretched or proteolytically degraded at these regions.
Cellular contractility is critical for branching morphogenesis of the lung. Inhibiting actomyosin-mediated contractility in whole lung explants decreases branching (Moore et al., 2005), whereas activating contractility increases branching (Moore et al., 2002). On a larger scale, lung development depends on mechanical distention, which is controlled in part by fetal breathing movements and fluid secretion into the lumen of the airways. These processes may cause local distentions at growing buds which stretch out the surrounding basement membrane. Several MMPs are expressed during lung development, and treatment with a broad-spectrum MMP inhibitor blocks airway branching (Kheradmand et al., 2002). Furthermore, knockout of tissue inhibitor of metalloproteinase (TIMP)-3 function disrupts lung branching morphogenesis (Gill et al., 2003). However, the mechanism of action is unclear as a large majority of MMP knockout mice develop normally without any apparent lung phenotype (Parks and Shapiro, 2001). An exception is the MT1-MMP/MMP14 knockout mouse, which shows normal lung branching morphogenesis (Oblander et al., 2005) but has defective alveolar development (Atkinson et al., 2005).
Defects in lung development can result in a hypoplastic lung (with too few branches) or abnormal alveolar structures, both of which reduce the surface area for oxygen transport and thereby disrupt lung function. Many of these defects arise from alterations in the mechanical environment of the thoracic cavity. Normal development of the lung requires fetal breathing movements, an adequate volume of space within the chest cavity, and sufficient volumes of fluid within and surrounding the growing lungs. Transecting the spinal cord at the level of the phrenic nerve prohibits fetal breathing and disrupts lung development in rabbits and sheep (Fewell et al., 1981; Wigglesworth and Desai, 1979). During fetal breathing, the diaphragm contracts and increases the volume of the thoracic cavity, which stretches the developing lungs; this stretching may influence lung development by increasing cell proliferation (Fewell et al., 1981; Liu et al., 1992). Combined with upper airway resistance, fetal breathing movements maintain transpulmonary pressure by controlling the volume of fluid within the lungs. Decreasing fluid or pressure leads to pulmonary hypoplasia, whereas increasing fluid leads to hyperplasia (Alcorn et al., 1977; Kizilcan et al., 1995; Moessinger et al., 1990).
In humans, there are several conditions that can lead to fetal pulmonary hypoplasia, a major cause of respiratory insufficiency of the newborn (Harding and Hooper, 1996). Congenital diaphragmatic hernia (CDH) is a malformation of the thoracic diaphragm which permits organs of the abdominal cavity to bulge into the chest cavity. The herniated organs are thought to compress the developing lung. CDH affects approximately one in every 2500 births, with mortality rates of 10–35% for live births and possibly higher when accounting for fetal demise (van den Hout et al., 2009). Patients with CDH present with pulmonary hypertension shortly after birth. Fetal pulmonary hypoplasia can also result from abnormally low levels of amniotic fluid (oligohydramnios) resulting from premature rupture of membranes (PROM). Conversely, fetal defects characterized by accumulation of fluid, including pleural effusions or fetal hydrops, result in pulmonary hypoplasia as well, presumably from the increased pressure on the growing lungs.
The cardiovascular system is the major transport system of the body, conducting oxygen, cells, nutrients, and waste products to their final destinations. The heart is the first organ to develop; severe defects in heart development are one major cause of embryonic lethality. In the human embryo, the heart begins beating at ~21 days post-conception. The heart pumps throughout its morphogenesis, and its constituent cells are exposed to multiple mechanical stimuli, including wall shear stress, transmural pressure, and stretch. The development of the heart proceeds via sequential processes of cardiomyocyte specification (day 15 in the human embryo; HH stages 5–9 in the chicken (Hamburger and Hamilton, 1951)), formation and looping of the heart tube (days 20–28 in the human; HH stages 10–24 in the chicken), development and growth of the chambers (days 28–32 in the human; HH stages 19–24 in the chicken), and development of cardiac cushions, valves, and septae (days 32-birth in the human; HH stages 25–34 in the chicken)(Bruneau, 2008; Manner, 2009).
The mechanical forces accompanying heart development have been most closely examined in chicken and zebrafish embryos. Cardiac looping morphogenesis in the chicken closely resembles that in the human embryo (Kirby, 2007). During looping, the initially straight heart tube undergoes bending and torsion to form the basic topographical pattern of the multi-chambered heart (Figure 1). During the first stage of looping, c-looping, the heart tube bends ventrally and twists toward the right (dextrally) to form a c-shaped tube. During s-looping, the c-shaped tube shortens and twists into an s-shaped tube. Bending can occur normally even in embryonic chicken hearts isolated in vitro (Manning and McLachlan, 1990), and is thought to result from actomyosin-mediated changes in cell shape (Latacha et al., 2005). As the heart tube bends, myocardial cells flatten at the outer convex curvature, whereas others elongate and thicken at the inner concave curvature (Manasek et al., 1972). Inhibiting actin polymerization blocks bending in vivo and in isolated hearts (Latacha et al., 2005), consistent with a cell shape-driven process.
Changes in cell shape also appear to be responsible for the ballooning process that sculpts the chambers of the heart. Ballooning involves expansion and bulging of the linear walls of the looped heart tube into bean-shaped chambers, again with a convex outer curvature and a concave inner curvature. High-resolution imaging of the developing zebrafish heart revealed that cells of the outer curvature flatten and elongate relative to those of the inner curvature, which remain cuboidal (Auman et al., 2007). Physical forces resulting from normal blood flow were shown to be required for cell shape change, as disrupting flow either genetically or pharmacologically inhibited the flattening and elongation of cells in the outer curvature (Auman et al., 2007). It is still unclear how the cells are sensing these forces and transducing them into alterations in cell shape.
Forces from blood flow also regulate development of the heart valves. After looping is completed in the zebrafish embryo, cardiac cushions form at the atrioventricular (AV) boundary, the separation between the ventricle and atrium chambers (Eisenberg and Markwald, 1995). Communication between the myocardial and endocardial layers at the AV boundary leads to an EMT of the endocardial cells, forming cushions that then differentiate into the flaps of the AV valve. Quantitative in vivo imaging of hemodynamics in the developing zebrafish heart revealed the presence of high-shear (>1 dyn/cm2) vortical flow during and after valvulogenesis (Hove et al., 2003). Valves fail to form in mutant embryos that lack a heartbeat (Bartman et al., 2004), and mechanically blocking blood flow disrupted looping of the heart tube and valve formation (Hove et al., 2003). Fraser's group recently showed that disturbed flow patterns, rather than shear stress per se, are responsible for activating the flow-responsive genes that direct valvulogenesis (Vermot et al., 2009). Specifically, reversing flows activated Kruppel-like factor (Klf)-2a in the endothelium in valve-forming regions, and morpholino-mediated knockdown of Klf2a disrupted heart valve formation (Vermot et al., 2009). How endocardial cells sense and respond to disturbed flow is unclear, but extensive analysis of hemodynamic signaling in the endothelium of adult vessels yields several promising clues. Oscillatory flow patterns with recirculation tend to form at branch points within the vascular bed and distal to stenoses, and these hemodynamic forces have been implicated in the local development of atherosclerosis (White and Frangos, 2007). Endothelial cells in regions of disturbed flow in vivo and in culture exhibit increased proliferation, apoptosis, junctional permeability, and oxidative stress (Hahn and Schwartz, 2008) resulting from sustained production of reactive oxygen species (ROS), which themselves act as mediators of EMT (Radisky et al., 2005). It remains to be determined whether and how disturbed flows signal to endocardium through ROS.
Formation of the heart valves proceeds similarly in amniotes. In the chicken embryo, the AV and outflow tract cushions start to emerge as expansions of cardiac jelly, the ECM in between the myocardium and endocardium that is rich in hyaluronan and chondroitin sulfate proteoglycans. The myocardium then induces an EMT of the endocardium, causing the latter cell population to migrate into the cushions and form the mitral and tricuspid valves as well as the atrial and ventricular septae (Martinsen, 2005). The Rho GTPase effector, Rho kinase (ROCK), is expressed in the migrating population and treatment with the ROCK inhibitor Y27632 blocks valve formation in the chicken embryonic heart (Sakabe et al., 2006). Furthermore, the basic helix-loop-helix (bHLH) transcription factor Twist1 is expressed in the mesenchyme of the developing heart cushions (Ma et al., 2005), and induces proliferation and migration of endocardial cushion cells (Shelton and Yutzey, 2008). Mechanical stress activates ROCK (Chiquet et al., 2009; Wozniak and Chen, 2008) and induces expression of Twist in Drosophila embryos (Desprat et al., 2008a; Farge, 2003), suggesting that hemodynamic forces may induce EMT and heart valve formation through Rho GTPases and Twist.
Congenital heart defects affect approximately one percent of newborn infants (Hoffman, 1995a), making them the most common type of birth defect and a major cause of infant mortality in the first year of life. Abnormalities in the early stages of heart development result in embryonic lethality (Hoffman, 1995a), so the majority of heart defects in infants that make it to term result from abnormal development of the valves and septae (Hoffman, 1995b). Members of the Notch family have emerged as key regulators of valve development, and disruptions in Notch or Jagged signaling can lead to bicuspid aortic valve (BAV) (Garg et al., 2005), a defect in which the aortic valve has two leaflets instead of three. Notch is thought to act by promoting EMT in the cushions during valve formation (Timmerman et al., 2004). It will be interesting to determine the links, if any, between mechanical stress, Notch, and EMT in cardiac morphogenesis.
The study of mechanobiology requires understanding of the three main aspects of cell and tissue mechanics: rheological properties, endogenous generation of force and response to exogenous mechanical perturbations. As a consequence of the dynamic nature of cells and tissues, however, these three aspects are often coupled (Bissell et al., 1982; Davidson et al., 2009; Ingber, 2003a; b), which poses a great challenge in precisely isolating the mechanical stimuli which cells sense and to which they are able to respond. To rigorously define the role of mechanics in developmental processes, the existing techniques must be refined and new sophisticated techniques introduced.
The vast majority of our current knowledge in developmental mechanobiology comes from studies performed in culture. Cell-generated mechanical forces were visualized for the first time nearly three decades ago, when Harris and coworkers demonstrated that fibroblasts locomoting on two-dimensional (2D) silicon membranes pull on their substratum, creating wrinkles (Harris et al., 1980). Monitoring the distribution and size of the wrinkles allowed semi-quantitative characterization of endogenous cellular forces. Approximately two decades later, Pelham and Wang introduced the use of bioinert polyacrylamide (PAA) gels with tunable stiffnesses to study the effect of external mechanical perturbations on cellular behavior (Pelham and Wang, 1998). These two techniques later evolved into traction force microscopy (TFM), which incorporates fluorescent beads into the PAA substrata to visualize displacements due to cell-generated traction forces. TFM allows study of both cellular response to external mechanical changes and measurement of endogenous forces, producing force maps with subcellular resolution (Dembo and Wang, 1999; Pelham and Wang, 1999). An alternative technique involves plating cells on top of an array of elastomeric micrometer-scale posts. Here, the cell-generated forces are computed from the deflection of the elastic posts, which reduces the mathematical complexity of TFM (Tan et al., 2003).
Sophisticated lithography-based microfabrication methods developed in the past decade have enabled researchers to control the magnitudes of mechanical stress experienced by isolated single cells by restricting cell spreading (Figure 2)(Chen et al., 1997). Furthermore, these methods were critical in demonstrating that tissue geometry controls the distribution of mechanical stress over cellular monolayers (Nelson et al., 2005). This discovery inspired the use of microfabrication as a means to precisely control tissue geometry, and consequently spatial patterns of stress within cell monolayers, allowing further insight into the role of mechanics as a potential regulator of biological processes. Indeed, microfabrication was instrumental in demonstrating that mechanical stress spatially patterns important developmental processes such as apoptosis (Chen et al., 1997), proliferation (Nelson et al., 2005), differentiation (Kilian et al., 2010; McBeath et al., 2004) and epithelial-mesenchymal transition (Gomez et al., 2010; Nelson et al., 2008b), and unveiling some of the underlying mechanisms of mechanotransduction (Gomez et al., 2010).
Several recently-developed techniques for microfabricating three-dimensional (3D) epithelial tissues hold promise in addressing questions in developmental mechanobiology with a higher level of physiological and histological realism (Nelson and Tien, 2006). We recently developed an approach that relies upon replica molding of biomimetic hydrogels around elastomeric stamps to generate cavities of micrometer-precision size and geometry (Nelson et al., 2008a; Nelson et al., 2006). Cells subsequently inserted into the cavities self-organize into 3D tissues. The main advantages of engineered 3D epithelial tissues include the accessibility and ease with which mechanical modulations can be performed and endogenous mechanical characteristics interrogated quantitatively. The concentration of the hydrogels or the extent of crosslinking can be varied to modulate matrix stiffness, thereby altering the mechanical signals originating outside the cells. Conversely, molecular or pharmacological approaches can be taken to perturb mechanical elements within the cells, including integrins, intercellular adhesion and cytoskeletal proteins. In a fashion similar to the microfabricated 2D tissues, the patterns of mechanical stress can be controlled by the 3D geometry, which would in this case allow researchers to draw correlative and causative links between the spatial profile and magnitudes of mechanical forces, on one hand, and sites and extents of 3D morphogenetic processes within the tissues, on the other. Furthermore, matrix deformations due to tissue morphogenesis can be readily measured and used to quantify morphogenetic forces in a spatially and temporally resolved manner.
Although studies in culture have elucidated the mechanical control of basic cellular processes, a comprehensive understanding of developmental mechanobiology will not be secured until the contribution of mechanics is confirmed in vivo. A first step in that direction could be to confirm that mechanical forces of magnitudes correspondent to those required for cellular response in culture also exist in vivo. Such an effort, nevertheless, requires precisely defined lower and upper limits of force which cells can sense, and information of that nature is generally lacking in the field. The typical approaches in mechanobiology modulate the magnitude of endogenous cellular force by varying the compliance of the substratum (Alcaraz et al., 2008; Engler et al., 2006; Paszek et al., 2005; Wozniak et al., 2003) or the geometry of the tissue (Gomez et al., 2010; Nelson et al., 2005; Nelson et al., 2008b). The magnitudes of the forces that accompany or are needed to elicit various cellular responses are summarized in Table 1, where available. Future work should focus on defining the range in which these forces have developmental relevance in culture and determining whether such forces and the corresponding biological effects are also present in vivo.
As argued above, a comprehensive understanding of the mechanical control of development requires techniques to apply and measure mechanical force in vivo. The large plastic deformations, fragility and inaccessibility of developing tissues, however, further complicate the already challenging task of studying developmental mechanobiology in culture. In the following sections we describe some of the techniques used to measure and perturb the mechanical properties of and forces generated within embryos and embryonic tissues.
The mechanical properties of cells and tissues are determined by the properties of individual molecules within the cells, their organization into subcellular structures and the organization of cells and the ECM (Davidson et al., 2009). Measuring the mechanical properties of materials generally involves application of a known force and assessing the resulting deformation. Micro-indentation techniques, which rely on the same fundamental principles as atomic force microscopy, apply nano-Newton force via a thin cantilever beam on small fragments of embryonic tissue and measure the resulting tissue displacement. Such methods have been successfully used to measure the mechanical properties of chicken embryonic heart tissues (Zamir and Taber, 2004a; b). More specifically, Taber and coworkers fitted force-displacement and surface displacement experimental data to the strain-energy density function to determine the material parameters of the myocardium and cardiac jelly of the chicken heart during cardiac looping at the c-shaped stage. In addition, they found that the myocardium at this stage experiences substantial residual stresses, which may be of morphogenetic relevance.
Micro-aspiration methods are non-destructive approaches which apply known negative suction pressure to aspirate small portions of embryonic tissue into microchannels. The elastic modulus of the tissue is calculated from its displacement into the channel, the radius of the channel and the applied pressure (von Dassow and Davidson, 2009). Micro-aspiration techniques have been used to monitor changes in stiffness during the gastrulation of whole Xenopus laevis embryos (von Dassow and Davidson, 2009). Embryonic tissue stiffness has also been extracted from uniaxial and parallel plate compression studies. One such study measured the resistive force that arises upon a given compressive deformation and uses the force, the compressive strain and the cross-sectional area of the spherical explant to compute the stiffness of the Xenopus laevis embryo during key stages of its development (Zhou et al., 2009). Similar studies have described the mechanical properties of cellular aggregates from chicken and sea urchin blastula (Davidson et al., 1999; Forgacs et al., 1998). The main limitation of these methods is the requirement of direct contact between the cells and the physical probe at the time of measurement. However, in their physiological environment cells are typically inaccessible to physical probes, as they are often embedded in ECM or surrounded by other tissues. Microrheological approaches circumvent these problems by injecting nanoparticles into individual cells of interest and tracking the particles as the embryo develops. The Brownian motions recorded are informative of the micromechanical environment surrounding the particles, allowing quantification of the viscoelastic properties of the cells with high spatial and temporal resolution (Daniels et al., 2006).
Defining the spatial and temporal profiles of mechanical forces within the developing embryo and correlating them with the spatiotemporal dynamics of developmental processes arising therein would likely shed light on the role of mechanical signals in development. In addition, quantitative knowledge of embryonic forces would help confirm the in vivo relevance of mechanically-controlled biological phenomena reported by culture studies. Therefore, methods to precisely quantify mechanical forces in vivo are required.
An indirect method to examine the mechanical profile of developing embryos is to visualize molecular markers of mechanical tension. Researchers have commonly monitored the spatiotemporal distribution of ROCK (Daley et al., 2009), phosphorylated myosin light chain kinase (Daley et al., 2009; Wang and Riechmann, 2007) and myosin II (Martin et al., 2009), all associated with cellular contraction, as means to map the mechanical forces within the embryo. A more direct method to estimate mechanical force in vivo is laser ablation. Here, a tightly focused laser beam is used to disrupt presumably tensed subcellular structures (Hutson et al., 2003; Rauzi et al., 2008). Shortly after the ablation, the structures of interest and their surroundings relax and displace at a given rate. The relaxation rates of distinct locations within the embryo provide a relative measure of the mechanical tension present at each location before the ablation. The main limitation of this technique is the fact that, while allowing comparison of mechanical force between different spatial and temporal points in the embryo, it does not furnish an absolute measure of force, which necessitates knowledge of the generally elusive local mechanical properties. Optical coherence tomography (OCT) is a technique that measures backscattered light as a function of depth, allowing 3D subsurface imaging of biological samples with high resolution and sensitivity (Filas et al, 2007). Filas et al have used OCT to determine the spatial and temporal variations of myocardial strain of the embryonic chicken heart during the first phase of looping, i.e. as it forms a c-shaped tube. More specifically, microbeads placed on the myocardium were tracked during the process and their displacements used to calculate the longitudinal, circumferential and shear strains in seven different regions of the heart. The study, however, does not calculate the corresponding forces, most likely due to the complex geometries and constitutive relations of the morphogenetic tissue.
Measurement of hemodynamic forces in vivo has been considerably more successful. Characterization of hemodynamic stress generally involves determination of the velocity profile of blood within the vessel of interest and subsequent calculation of the corresponding shear stress by using Newtonian fluid mechanics theory (Friedman and Giddens, 2005). Researchers have developed methods for rapid (440–1000 frames s−1) 3D imaging of the embryonic mouse and zebrafish heart using laser slit-scanning and line-scanning confocal microscopy (Hove et al., 2003; Jones et al., 2004; Liebling et al., 2006). These techniques have been instrumental in demonstrating that both the early (37 hours post-fertilization) and later (4.5 days post-fertilization) embryonic zebrafish heart experiences shear forces above the threshold necessary to induce gene expression and cytoskeletal changes in cultured endothelial cells (Davies et al., 1986; Hove et al., 2003; Olesen et al., 1988). The optical inaccessibility of large blood vessels makes confocal imaging approaches unfeasible for hemodynamic measurements in adult animals and humans. The blood velocity profile here is either directly measured using phase-contrast magnetic resonance imaging (MRI) (Barker et al.; Hollnagel et al., 2007) or is modeled numerically by performing computational fluid dynamics on vessel geometries reconstructed from MRI or ultrasound images (reviewed in (Steinman, 2002)).
In addition to methods to measure mechanical force, establishing causative links between the mechanical environment and developmental processes in vivo requires methods for mechanical manipulations. A simple approach to impose mechanical stress globally is uniaxial compression. Submitting the Drosophila embryo to a 10% lateral uniaxial deformation has been used to demonstrate mechanically-driven expression of Twist, an important morphogenetic protein (Farge, 2003). More localized application of mechanical stress is possible with microinjection of magnetic nanoparticles in specific regions of the embryo. The particles are manipulated by magnetic tweezers, causing them to move and impart mechanical stress on the host cells (Desprat et al., 2008b). Endogeneous mechanical stresses within embryos and stress arising due to morphogenetic movements are routinely ablated with subcellular resolution using laser microsurgery (Desprat et al., 2008b; Supatto et al., 2005).
Understanding how developing embryos generate and respond to force, as well as their mechanical properties will undoubtedly shed light on the mechanisms whereby physical cues regulate developmental processes. Comprehensive description of embryo mechanics, however, is hindered by a number of technical limitations, but perhaps most strongly by the dynamic nature of living cells and tissues, which are both influenced by the environment and in turn themselves influence their surroundings. Therefore, developing sophisticated methods that address questions in mechanobiology in a targeted and controlled manner is of great importance.
Work from the authors' laboratory is supported in part by grants from the NIH (GM083997 and CA128660), Susan G. Komen for the Cure, and the David & Lucile Packard Foundation. C.M.N. holds a Career Award at the Scientific Interface from the Burroughs Wellcome Fund.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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A slightly detached, short-sleeve button-down top that allows room for a tie closure adds unique character to this knee-length dress. Cut in a soft, lightweight cotton, it features a full skirt with soft pleats and hidden side pockets. Worn with Gimli Slide and Soiree Crossbody Cirapp 100% Cotton 63.5cm / 25" from natural waist Style #: 10364DVF Color ID: WHITE
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DVF's nude Zarita dress offers a youthful take on ladylike lace. The pretty sheer fabric is layered over a solid underlayer - crafted from sculpting silk-jersey -and cut with a flattering boat neck V-shaped back cropped sleeves and a thigh-grazing hem. Wear it to weddings and parties with simple same-tone pumps.


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Tuesday, May 1, 2007
My Turbinates for a Quilt
My sister Paula is married to Dennis Thompson, an ear-nose-throat surgeon. When my snoring was bad enough to interfere with John's sleep, Dr. Den was a great resource. He first used Pilar implants in my soft palate, but my snoring persisted. So then he shrank my turbinates with radio frequency. For the first time in years I think I am breathing clearly and well. When Paula and Dennis purchased a building for their practice in St. Joe, MI, Paula of course handled the decorating (as well as everything else) of this new space. There's a wonderful tall wall in a central spot, and Paula asked for a wall hanging. What a great opportunity to begin to repay Dennis for his services!
Armed with some mini-quilts, sample wall hanging, and fabrics in Paula's palette of browns, neutrals, and blues, I drove to St. Joe. They both spotted a design they liked, and Dennis seems to like bold graphic lines. Paula and I then hammered out the specific fabrics. The result is lovely - contemporary, quiet, eye-catching. There will be a better photograph once this is hung in its space.
2 comments:
I love this patchwork! You are best!
Leja from Sweden
- May 18, 2009 at 6:15 AM
Patchwork from Sweden. Leja
- June 3, 2009 at 6:28 AM
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text: I'm not a magician, much less a genius, but only a matter of human understanding within the squad of Milan. I do not think air turns positive for the Shaarawy? As I had already mentioned once that with the arrival of Balotelli, the coach with all the staff have forgotten the man who led them to an excellent level in the standings in the league? The media also on their side continued to advertise more Mario Balotelli. In short, two cocks in the henhouse, they should not continue to live together. El Shaarawy has seriously need a moral support from especially by his coach to be able to return to the levels of before. From the rest is a very simple and modest player and in my opinion deserves to be helped more. If you can not marginalize a young talent! . Congratulations to the young Pharaoh of Milan and the Italian National Team.


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Short on time but want to experience the top of the park? This trip offers one day unguided walking and one day guided kayak.
Do you have only two days to experience the Abel Tasman and want to see the Northern end of the park? This trip has been designed for those wanting to experience the top of the park. One day unguided walking and one day guided kayak.
We have taken our popular Three Day Tiki and broken it into the first two days. This is 'kiwi tikiing' at its best: one day unguided walking, one day guided kayak. Water taxi to Totaranui and spend the first day walking in the northern section of the park. After crossing the Awaroa Lagoon spend a night at Awaroa Glamping, Awaroa Bay. Day two sees you paddling on our Marine Reserve Tour, after you start the day with a 1hr 10min walk to Onetahuti where you will meet your guide. At the end of the day return to Marahau by Water Taxi.
We normally are able to secure dorm space at Awaroa Glamping but please note at peak times this may not be possible. In the event of this we can discuss alternative accommodation in the National Park.Book This Trip Download Itinerary & Map


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This week's Nintendo Download includes the following featured content:
This Smart News Release features multimedia. View the full release here:
Skillfully pilot the Graceful Explosion Machine, a fighter ship armed with a ludicrously overpowered quad-weapon array. Lost in deep space, you'll shoot, dash and combo your way through jewel-hued alien worlds, fighting crystalline enemies to find a way home. (Photo: Business Wire)
You can also redeem points to get a Fire
Emblem Echoes: Shadows of Valentia digital wallpaper for
your smartphone or PC. For more info, check out the latest My Nintendo
news here.
Also new this week:
In addition to video games available at retail stores, Nintendo also
offers a variety of content that people can download directly to their
systems. Nintendo adds new games weekly to Nintendo eShop on the
Nintendo Switch console, the Wii U console, and the Nintendo 3DS family
of systems.
Nintendo eShop is a cash-based service that features a wide variety of
content, including new and classic games, applications and demos. Users
can add money to their account balances by using a credit card or
purchasing a Nintendo eShop Card at a retail store and entering the code
from the card. All funds from one card must be loaded in Nintendo eShop
on Nintendo Switch, Wii U or the Nintendo 3DS family of systems, but can
be used in any Nintendo eShop if the systems are linked to a single
Nintendo Network account.
The Wii Shop Channel offers games and applications and uses Wii Points™,
which can be purchased via the Wii Shop Channel. The Nintendo DSi™
Shop offers games and applications and uses Nintendo DSi Points™.
Click here
for details about the recent Nintendo DSi Shop service change.
Remember that Nintendo Switch, Wii U, Wii, New Nintendo 3DS, New
Nintendo 3DS XL, Nintendo 3DS, Nintendo 3DS XL, Nintendo 2DS and
Nintendo DSi feature parental controls that let adults manage some of
the content their children can access. Nintendo 3DS players who register
a Nintendo Network ID gain access to free-to-start games and free game
demos from Nintendo eShop, and also get the latest news and information
direct from Nintendo. For more information about this and other
features, visit http://www.nintendo.com/switch,
or http://www.nintendo.com/3ds.
Note to editors: Nintendo press materials are available at http://press.nintendo.com,
a password-protected site. To obtain a login, please register on the
* Note that additional accessories are required for multiplayer mode,
and are sold separately.
View source version on businesswire.com: http://www.businesswire.com/news/home/20170406005332/en/
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ITEXPO: February 14 - 16, 2018 | Fort Lauderdale, Florida
Real Time Web Solutions Conference: February 14 - 16, 2018 | Fort Lauderdale, Florida
IoT Evolution Expo : January 22-25, 2018| Orlando, Florida


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Why Bitcoin will not kill PayPal
To a casual observer, PayPal might seem like a dying payment program. It was onc...
Duke Energy Renewables enters New York, purchasing one of the largest solar projects in the state from Invenergy
Jasper Capital International Becomes Second China-Based Signatory to Hedge Fund Standards Board
Jasper Capital International (''Jasper'') has become the second China-based sign...
International Payments: Remittances From Migrants
See how remittances via international payment solutions by migrant workers have ...
SWIFT Response To Cyber Attacks | International Business Payments
Learn why businesses making international business payments via wire transfer sh...
A Passion for Real Estate and Architecture
McKafka Development Group is a fully integrated real estate group with developme...
EWM - Own the life that fits your style
Founded in 1964, EWM Realty International (EWM) is one of the largest real estat...
Man About Town
Founded by Andrew Heiberger in 2010, Town Residential has cemented its position ...
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By Job Title
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Registered in England and Wales.
No. 07400916
1st Floor, Suite F,
The Maltsters,
1-2 Wetmore Road,
Burton on Trent
Staffordshire, DE14 1LS


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Tours of Ballett Zurich at the Bolshoi theatre: Peter Tchaikovsky "Nutcracker and the Mouse King" (ballet in 2 acts) will take place on 28 and 29 November 2017
Stars of the Stars perfomance. Concert Rimsky-Korsakov. Stravinsky. Performed by National Philharmonic Orchestra of Russia and Grand Choir "Masters of Choral Singing". Conductor – Vladimir Spivakov. The concert will take place at Tchaikovsky Concert Hall on 31 October 2017 (Tue), 19:00
Stars of the Stars performance. Concert Mozart. Mahler. Performed by Tatarstan Symphony Orchestra and Philipp Kopachevsky (piano). Conductor – Alexander Sladkovsky. The concert will take place at Tchaikovsky Concert Hall on 29 October 2017 (Sun), 19:00


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40394 North South Newport Drive, Antioch, Illinois
$389,0003 Beds3 BathsActive
$389,0003 Beds3 BathsActive
40394 North South Newport Drive, Antioch, Illinois 60002 - Much more than a home, it's a whole new lifestyle at Newport Cove! This absolutely gorgeous 3 Bedroom 3 Bath home features an open spacious floor plan with 10-foot ceilings. Spend relaxing afternoons on the deck with views of the water. You'll love this premium location backing to nature. Large living room opens into dining room. Gourmet kitchen has stainless steel appliances, granite counters, breakfast bar plus breakfast room. 1st floor den or bedroom. Master bedroom with spa bath: whirlpool, separate shower, dual vanity & walk in closet with custom built-in's. Easily entertain in walkout lower level: huge family room, another bedroom or home office, 3rd full bath. Storage room plus ample closet space. Luxury finishes include hardwood floors, 8-foot tall doors, oversized trim. Pella brand windows with warranty. Lake rights to Bluff Lake, enjoy Chain O'Lakes boating & fishing. Walking/golf cart pathways to lakefront park with marina, gazebo, patio. HOA covers lawn care, snow removal.
View Virtual Tour
Listing Courtesy of Better Homes and Gardens Real Estate Star Homes


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More than 2.5 million commuters, mostly transients from outlying provinces such as Bulacan, Cavite, Batangas, Rizal and Laguna will be severely affected once the MMDA implements its capricious plan of relocating provincial bus terminals away from their present locations.
Ninety one percent, says Elvira Medina, president of the National Commuters Protection, of commuters is against the bus relocation plan, saying that this will cost them more transport money, more time to go to their workplaces and will probably affect bus fares, since government, thru the MMDA will impose a 350php terminal fee for every bus that uses the terminals.
In an interview, Medina says the MMDA plan is actually okay if Metro Manilans are prepared. Had government built an integrated intra- and inter-city modal system, such schemes would have been possible. In our time right now, such ideas will just bring chaos and confusion.
MMDA plans to implement a unified bus terminal system. The plan says that provincial buses plying the North Luzon route should use the Trinoma terminal. Those coming from Cavite and using Coastal mall, should use the Uniwide bus terminal. The festival mall in Ayala Alabang will serve those coming from the Southern corridor while in the East, there is a plan to rent out the Robinsons East mall.
What the scheme will do is this:
1. If you're a commuter coming from, say Bulacan, you will need to ride a provincial bus going to Trinoma. From Trinoma, you will then ride another public transport, either a city bus or a jeepney or a tricycle. Imagine the chaos this will cause since: extra city buses will then converge in this area aside from additional taxis, mini-buses, jeeps and private vehicles which will all go to this singular area to fetch those commuters.
2. People say, commuters should use the MRT. The MRT can only accommodate 850,000 passengers in a single day. Imagine the long lines and chaos if that extra 2.5 million transient commuters use the MRT line.
3. Of course, several commuters will use taxis. Additional taxis going to this area will definitely add more pressure and vehicular volume. This segment of EDSA will definitely turn into a chokepoint, instead of freeing EDSA of traffic.
4. What about commuters who carry cargo? Small-time vegetable dealers use provincial buses as their primary freight mode of transport. where will they place their cargoes? unto jeepneys and taxis? City buses and MRT do not have cargo bays.
In sum, this MMDA plan is headed for a disaster. MMDA plans to have a dry-run this Thursday and operationalize the Southern bus terminal by August 6.
I admire MMDA Chair Francis Tolentino. Pero, sir, you are creating a potential public riot if you implement this.
Even if this pushes through, this will only de-clog EDSA by 6% since all provincial buses comprise about 6% of the total volume of vehicles plying the highway, once, twice and thrice a day.


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210 16th Street, St Augustine, FL
$259,0003 Beds2 Baths
$259,0003 Beds2 Baths
210 16th Street, St Augustine, FL 32080 - Beautifully updated Anastasia by the Sea condo in the heart of St. Augustine Beach. Corner unit full of natural light offering 3BR's and 2BA's with rear screened lanai to relax and enjoy the ocean breeze. Welcomed by the warm laminate floors, this upgraded unit offers a completely remodeled kitchen that includes granite tops, upgraded cabinetry, stainless appliances, tile back splash and eating bar. Recent bath vanities with granite tops! Community pool with pavers, clubhouse with screen porch! Only a short stroll to sand at the end of 16th Street, walking distance to local restaurants, shops, pier, splash park and Wednesday morning farmer markets! Buildings recently painted and roofs replaced 3 years ago. Association in good standing. Monthly rentals are permitted.
Listing Courtesy of RE/MAX 100 Realty
"We are a locally owned and independently operated real estate business in St. Augustine, Florida, where Realtors shared ideals of exceptional personal service and expertise have joined together to offer a select group of clientele a level of assistance rarely found in the industry."


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The women's 'Catherine' dress from Diane von Furstenberg is crafted from a silk blend with slight sheen, and showcases a soft fluid silhouette with deep V-neck front, tuxedo collar and button up fastening. An all over, multi-coloured print adorns the fabric, and a waist-sculpting, mixed leather and fabric self-tie belt creates a feminine shape. Complete with long chiffon sleeves, gathering at the shoulders and a ruffle to the hem. - K.N.
95% Silk, 5% Spandex


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When you purchase a digital subscription to Cake Central Magazine, you will get an instant and automatic download of the most recent issue.
My 2nd Lightning cake...I like this one better than my 1st one.
Lightning Mcqueen
Cake looks great! Fantanstic job. :roll:
(if this is a private computer)
Yes please, register now!


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Spring semester is winding down for students living at apartments in College station. As a hardworking college kid, you're probably overwhelmed by the amount of writing, typing, charging, and caffeinating you've had to do as of late. Maybe you're thinking of ways you can make life a little easier on yourself next semester. If you're a senior, you've probably spent a few minutes telling an underclassman how they can better prepare themselves so they don't have to work quite as hard as you had to.
2500 Central Park is here to help you make your next semester of college a little easier. After you've worked all summer and saved up some dough, you might want to consider buying some helpful, student-focused technology. Whether you need a better way to wake up, a louder speaker for your study tunes, or simply a better, more functional backpack, the list below can help you out. Summer is fast approaching, and fall semester is probably the last thing on your mind, but it's never too early to start prepping for next year.
Here are the top gadgets to buy this spring to prepare you for next semester, courtesy of 2500 Central Park College Station apartments.
What exactly is an Aurabox, you say? In essence, it's a Bluetooth speaker with an LED front plate that flashes in time to the music. But wait, there's more. This cool little speaker also lights up to give you social media notifications. Plus, the LED display on the front can be customized to show your own pictures. If you're in for a long night of studying at your apartment, you might as well have this little gadget with you to add some fun.
Waking up for 8 a.m. classes is almost impossible, but it doesn't have to be that way. The Lumie Bodyclock is an alarm clock that wakes you up by gradually increasing the light in your room, so you wake up slowly rather than being startled awake by a blaring noise. This is scientifically proven to be a better way to wake up, and couldn't we all use a better start to our day than having an annoying alarm blast us out of bed?
Some people prefer to write notes by hand rather than lugging a laptop to class. This product is for those people, who often end up having to transcribe their notes into a Word document later on. This product automatically moves your handwritten notes to digital storage, saving you precious studying time down the road. All you have to do is make sure to take all your notes on the cool little electronic notepad, which actually lets you write on it with real ink. It sounds crazy, but it might be just what you need to set you apart from your peers in today's ultra-competitive college classrooms.
In college, you have to take every chance you get to charge your phone, laptop and tablet. With this backpack, you can put those worries to the side. You carry your backpack everywhere, so it might as well do everything for you. This backpack has charging ports, a Bluetooth speaker and even a cooler to hold your lunch in. It's not cheap, but it will save you from the dreaded "Battery Low" notification.
Once again, thanks to taking the time to read the latest 2500 Central Park blog. Check back in a few weeks for another post. Until then, be sure to follow 2500 Central Park on Facebook. That way, you'll be the first to know about all the awesome resident events and other updates from the apartments College Station students love coming home to.


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Your game will begin after the following advertisement
Recover a long-lost treasure to appease the sad monkey! Explore the world of Swampville in search of a powerful artifact. Monkey Go Happy Talisman features new hats, characters, and puzzles in the environment. Figure out exactly what to click to advance!
Monkey GO Happy Talisman is one of our selected Monkey Games.


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Please enter the email address associated with your User account. Your username will be emailed to the email address on file.
I would like to make a special donation to the Clallam County Historical Society to help in preserving our Clallam County history. Please use my tax deductible gift of $ for:


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Yokomo YZ2 Dirt/Carpet Side Plate Set
|Low Price:
$1.99 Flat Rate Shipping!
This item is eligible for $1.99 flat rate shipping Learn More
This item earns up to 10 VIP Reward Points Learn More
This is a replacement Yokomo Side Guard Set for use with the YZ-2 Dirt and Carpet Editions.
This product was added to our catalog on June 16, 2016.


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Tonight I had something that cannot be topped. I picked a tomato from my garden. It was warm and red. I sliced it, sprinkled a little sea salt and a twist of pepper on it. Just a drop of extra virgin olive oil.
no chef can do anything to make a better dish. just like all of us here I love going out to see what talented people can create. Nothing beats a great meal, done by a great cook.
However, the point of my post is that the earth gives us the marvelous bounty. We should all pause to enjoy those tastes that we have at our doors for a short time. In February, i would be begging for a cooked meal when only snow and gloom knock at our doors.
But for the next few weeks eat a tomato, find some good sweet corn, have a cantolope, revel n a warm juicy dripping peach. I live in lancaster co. so the bounty is at my door, but even those in the city can find these joyous pleasures now. enjoy it while it lasts


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Brief Biography of Thomas O'Connor
(Return to Biography title page)
Thomas G. OConnor owned the property known as Lot 2, Block 58 in the Prospect Hill area of Nevada City in the 1880s. He resided on the part of the land that fronting on Nimrod Street. It is possible that he also built a dwelling on Clay Street, for the 1883 Birdseye View map shows such a structure.
His wife was Julia and their children were: Thomas Gerald, Jr., Charles Francis, James Clarence, Annie Cecilia and Mary Marcella.
Thomas died as a young man in 1885. His obituary was published under the headline: Death of T. G. OConnor ( Obituary of Thomas OConnor: Nevada Transcript, 20 Oct 1885, page 3, Column 1). It described him as a well-known wood dealer in the county. It states that he had been ill with Brights Disease for a long time. His remains were removed from his home on Nimrod Street and taken to Grass Valley where his funeral was held at the Catholic Church.
Thomas OConnor had come to Nevada City from Grass Valley two years prior. Thomas G. OConnor had supplied wood to the Idaho Mining Company. Nevada County death records show that several of his children also died in Nevada County: Charles F, 31 Oct 1911, Annie C. 12 April 1894 and Thomas Gerald OConnor, Jr., 27 May 1926.
This biography is also available as a PDF file.
Please report broken links to the
This page last updated: 4 July 2015
This Web Page is sponsored by the USGenWeb Project in California, CAGenWeb.
© 1996 - 2015 ~ ALL RIGHTS RESERVED, Coordinator, Nevada County, CAGenWeb
Copyright pertains to all photos, graphics, web design, and the compilation and formatting of data extracted from historic records-- except for narrative and compilations written and performed by others as specifically noted herein.


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#{placeName} is great for:
#{othersAgree}% of others agree
Have your say...Add your opinion
Vienna is beautiful & lovable city. It's one of the most beautiful cities in Europe and the World for that matter. It wonderfully mixes time-aged culture and modern spirit. It has a terrific atmosphere of old culture and buzzing modern life. It's a city of ancient culture with a modern spirit. There is a lot to see and experience in Vienna: its museums and historical buildings. Remember, Vienna was an imperial city. I must add here also that Vienna is a well-governed city. Its public... Read more


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ResourcesHadarah's recommend resources for your spiritual journey
The Scriptures
Get yourself a copy of The Scriptures. I personally have a copy from the Institute for Scripture Research, but there are quite a few Messianic Hebrew scriptures available today. HalleluYah Scriptures is another one. And they have copies available for free/donation basis. The Scriptures come alive when The Names are restored. You'll start to see connections and contexts you never picked up on before. Start by reading the first five books (Genesis – Deuteronomy) The good news or "The Gospel" doesn't begin in the book of John. The first 5 books of what many call the Bible is actually The Turah or Torah, and it's the foundation for everything else you will read in The Scriptures. Everyone who was alive in the "New Testament" era used the "Old Testament" to teach and share the gospel. So study and understand The Turah first. No matter what version of The Scriptures you read, just make sure you read it. Too many of us have a million and one different "translations"of the Bible that sit on our shelves collecting dust. Don't allow yourself to accept a spoon fed religion. Pick up a copy of The Good Book and read it for yourself.
Living Branch Hebrew Assembly
Being an effective Witness
Friends & Family got questions about your beliefs? The video posted below is an excellent resource to help you become an effective witness. You'll go through scriptures and concepts with one of my fave Hebrew Teachers Lamadyahu Yashra'al. He will teach you a humble approach that you can use with any person who is curious or has questions about the truth. This video is courtesy of the Living Branch Hebrew Assembly on youtube. Enjoy the video below. I know it will enrich your life.
The Name of Yahuah. Three Resources
Information regarding the name of Yahuah.
People often ask me, How come you feel so sure about the name of Yahuah? How do you know it's not Yahweh, Jehovah, or one of the other names that people say? I'll start by saying this. There is a lot of confusion out there. There is also a lot of tradition that can often get in the way of the truth. I always say -I'm not a teacher, but if I know that something is true, I'm happy to share why I believe it. So I'll share some resources with you below on why I choose to believe our Heavenly Father's name is Yahuah.This is a blog post I wrote that references 3 resources for you to learn more about the name of Yahuah. Click here to read the post and begin your study.
The Path to Yahuah
Thepathtoyahuah.com is a great resource for studying the scriptures. Their website is dedicated to promoting the truth of Yahuah. They have a very useful article called "The Father's name made simple." They are a Messianic congregation, which means they believe the Mashiyach (Messiah), whose name is Yahusha, has come and will come again. I love studying with them online because The Teacher, Lamadyahu, is patient, humble, knowledgeable, and kind. I personally get a lot out of reading the study guides that he shares to go along with the videos he uploads on his youtube channel. You may also enjoy watching their videos live on Ustream.
This video is courtesy of Living Branch Hebrew Assembly and Medadyahu Yashra'al from living-branch.org . In all honesty the video below made a HUGE impact on my life. I hope it does the same for you. Enjoy.
Recent Blog Posts
music & motivation from setapartheart.com
I Trust in You. New Song. Special Announcement.
Hey, it's Hadarah. I have a new song for you... and a very special announcement. The new song is called "I Trust in You" and I pray that it will minister to your heart. No matter what is happening in life, and no matter how difficult things seem to get, it is our...
After you let go. Life Lessons.
Have you ever made the decision to let go of things in one area of your life? And then Yahuah challenged you to take things to the next level?
Learning to let go and let Yah lead. Life Lessons.
It's not always easy to let go, but if we can truly learn to slow down and let Yahuah lead, surely, He will lead us all the way Home.


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Sale of gold bullion
Category : Other Machines Posted : 31/01/2017 Price : Check with seller Location : Johannesburg(Johannesburg)
|UKBullion has best gold and silver coin offer for you. You can select wide range of Silver, Gold bullion, Sovereign at affordable price. Place your order and call us at 01902 623 256. Visit here:- https://www.ukbullion.com/


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Lake Shore Marketing
Lake Shore Marketing
Helpful (2)
I worked at Lake Shore Marketing full-time (More than a year)
I worked with Lake Shore Marketing last year for a couple months. I was in transition trying to figure out what I wanted to do with my career, and I found an opportunity with Lake Shore to develop and hone my marketing/promotions/teamwork skills. I learned a lot of things that have really helped in my current job: how to keep a positive attitude (even when I'm feeling down), managing my time and work ethic, how to read and work with different people, how to carry a, effective sales conversation with a customer, etc. If you are looking for a short term job that will give you a good kick in the pants to get started in what you really want, this is a great opportunity. You will learn a lot about yourself and how to be more successful in your life. I have my perfect job now because of some of the things I learned at Lake Short Marketing.
If you are not willing to give up your life and spend 10+ hours a day with minimum wage pay (unless you are able to make a ton of sales in the field EVERY DAY you are working) then this is not the job for you. While I learned a lot and got a lot of experience, I basically had to put my life on hold for the couple months I was there. I worked 6 (sometimes 7 or 8) days straight before having one day off, and each day I was working 10+ hours. On rare occasion I did work shorter days (6-8 hours). Those are just the hours I was paid for. You are also expected (you'll lose your job if you don't participate) to give a couple hours of your day for one-on-ones and team building activities that are outside of paid working hours once you are "promoted" to Trainer/Event Manager. You are told that your progression is completely dependent on your own work, but that's not quite true. You can't get into management until you build a team of several people that make it through several months of this kind of work schedule and all individually make it into the training position themselves. It's possible, but goodness, you have to give up a lot. You do get the "opportunity" to travel, but that usually means going to random town by yourself or one other team member (possibly even a stranger) in a different state and staying in a run down motel in the not so pretty part of town (oh, and the only thing paid for is your motel stay and some of your travel expenses). Overall, you do learn a lot and get some neat life opportunities, but unless you are willing to have this type of lifestyle, I wouldn't recommend this job for anything long term. I was always told "Not everyone can do this job" and that there were people who could do it and people who couldn't. It's not that I couldn't do the job; I wouldn't. My job was not and is not the most important thing in my life and not the measure of my success.
Advice to Management
Teaching and offering experience in self-managing and work ethic is great, but sending any human being out to stand in an aisle in a store for 8+ hours a day for 6-7 days straight and expecting a happy/productive/excited attitude the whole time is not the best way to do it. You would be able to build stronger teams and keep more people if you broke up the shifts more....like you say you are going to do during the interview process. Morning meetings are very motivating. They could be more productive though by being longer; less of a pump up session and more of a class-like setting. Since one-on-ones are required outside of work hours without pay, you could probably build these into the morning meetings more. You do a great job at teaching and motivating people. You don't do a good job at keeping people around. I know the purpose of long days and a rough schedule is to make people work hard for the sake of teaching them HOW to work hard...but there is only so much people can do. If you want to build stronger teams of people who want to stick around, start catering more to the "worker bees" and less to management, and start building company morale and not just work ethic.
Helpful (1)
Flexible hours, good company events, competitive salary
Bad management, lack of direction, project cancellations
I have been working at Lake Shore Marketing full-time (Less than a year)
It's not one person that makes this place a heck of an enjoyable place to work, it's every one's caliber and long term goals that make us work as a team for one another.
I work most of the weekends, but I sure make a lot of money on the weekends. At the management position though, I'm excited to have ore schedule flexibilities. My bosses hardly ever work weekends and they are always doing fun stuff!
Advice to Management
You guys are AWESOME!! Thanks for taking the time to get to know me personally and professionally. I've never had a "leader" as a boss; only a BOSS.
Helpful (3)
Marketing and Sales Techniques. Long term business plan
Where do I begin. After thinking I was one of the "few selected" for a second round interview, I quickly realized my mistake. After working 50 hours a week at a booth that we set up at Sams Club selling bamboo pillows(they are compfy but not worth the$) I realized I was getting paid minimum wage hourly and it was 100% commission based. I recently graduated and money wasn't the thing I was after, more of a reputable company to begin to work at and grow with. I worked my butt off for the owner and at the end of the day, the hours and standing around selling a product to strangers wasn't my idea of an "event coordinator" or even a "public relations assistant". If you are looking for a job in sales and marketing, you are better off selling cars at a used car lot...that might pay more!
Advice to Management
Be honest about not being 100& commisioned based. Also, the hours to work are ridiculous. Seven days a week on a rotating schedule with a day off once a month is ridiculous! and an event coordinator is not a person who goes and sets up a booth at Sams club!
Helpful (3)
It taught me a lot very quickly. And there was plenty of opportunity for me to grow in the business. As long as I was selling, I was making good money. A lot of high-energy, positive people around you shouting things like, "JUICE" at you. Juice is an acronym for Join Us In Creating Excitement. It's a good opportunity to gain sales experience.
Although it's a sales job, the trainers and management do everything to convince you that it isn't. They do this by making everyone memorize "marketing strategies" that repeat, "We're not sales people, we're promoters." You have to know this verbatim and repeat it evey day before heading into the field. The field is a Sam's Club, Kroger, or Meijer where you set up a booth and sell the product to the shoppers in the store. If you don't sell, you make minimum wage. If that happens you have to talk about what happened with your manager the next day and how to get better. What I disliked the most was being told that I'm self-managing, but then I was constantly micromanaged. I had to check in with my manager every day and break down my day every night, which meant sometimes being on the phone until 10pm. The work days are long, sometimes over 12 hours and weekends are a must. Absolutely no work-like balance.
Advice to Management
If it's a sales job, call it a sales job.
Helpful (17)
I worked at Lake Shore Marketing full-time (Less than a year)
Not a desk job, you talk to new people daily, get to be on road for a little time a day.
Horrible hours, they tell you get paid one amount when its really not even close. You have to meet at there office every mourning where you are greeted by four or five "trainers" who all give you a high five to try and boost your moral for this horrible day that is about to happen. Then you leave to go to your store where your booth is set up and play Billy Mays all day long, basically selling way overprice junk to random shoppers.
Advice to Management
Be upfront about the position when hiring, you tell people they are applying for event management (which is true after working at least 8 months) just say you are going to be in a booth for most of your employment.
Helpful (16)
I have been working at Lake Shore Marketing full-time (More than a year)
Lake Shore Marketing has an awesome team environment. All of the co-workers spend a ton of time together and everyone is friends. Upper management genuinely cares about the staff, and that really encourages a fun place to work every single day.
But if you have to work (which most of us do) Lake Shore is the place to be because it is so much fun.
Advice to Management
Work to keep the office fun and engaged. It is really nice to come into an environment that is not stagnant.
Helpful (28)
Lake Shore offers a unique opportunity for energetic and outgoing folks because the work involves constantly meeting new people. It is always great to connect with the clients and customers, and since the team is a very social group there is always something fun going on.
Due to the nature of the space, the work is somewhat demanding. However, if you are willing to stick to it then the hard work will pay off. Your co-workers will always be there to help you and train you if there is anything that you need.
Advice to Management
Do more community service or team building events so that everyone has an opportunity to hang out outside of work!
Helpful (27)
I have been working at Lake Shore Marketing full-time (Less than a year)
This is not a huge company with tons of politics and red tape that will prevent you from getting promoted quickly. Many people have moved from entry-level to management in less than a year (think 6 to 8 months), and that same opportunity is literally available to every single applicant that walks through the door.
Hard work is required to excel at this job. If you are expecting a company that allows you to screw around on the Internet all day then this is certainly not the place for you. However, if you are a tenacious individual then this is a huge opportunity for you.
Advice to Management
You are awesome! Thank you so much for all of your support!
Helpful (21)
I worked at Lake Shore Marketing full-time
You are desperate for money.
I applied for the position because the advertisement said the position was for event planning. I showed up for the interview and realized that the position was really just a sleazy sales positions. You have to travel to SAMs clubs across Indiana without getting money back. They also tell us that we are payed on commission which is True only if you sell a ridiculous amount of product. They would teach us sleazy sales techniques.The people who worked around me fell for all the stupid stuff they say like, "people who go to SAMs club are rich because they can pay for a SAMs club membership so you will obviously be able to sell to them". This company does not have any values. They lie to their employees and customers.
Advice to Management
Please get some values.
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Lomachenko vs Marriaga live stream.Vasyl Lomachenko vs. Miguel Marriaga fight prediction, preview, boxing odds.Date: Saturday, Aug. 5,Location: Microsoft Theater, Los Angeles, California,Time: 10 p.m.TV: ESPN.watch free now.
A free night of boxing will be streamed and broadcast by ESPN and the ESPN App, all culminating in the televised bout between Vasyl Lomachenko and Miguel Marriaga on Saturday. Lomachenko is one of the best super featherweights in the world, and Marriaga is a high-level gatekeeper who will determine which direction Lomachenko's career goes at this important juncture.
Saturday's undercard features several high-level bouts. The ESPN-televised card begins at 10 p.m. ET and includes the Lomachenko fight as well as a matchup between Ray Beltran and Bryan Vasquez in the lightweight division. Other undercard bouts are live-streamed via ESPN3 and the ESPN App beginning at 7 p.m.
Lomachenko will be defending his WBO title, and is hoping to improve to 9-1 on his career. He has six knockouts, and one split-decision loss to Orlando Salido back in 2014. Lomachenko has wanted a rematch with Salido, especially because many thought the decision was controversial. That fight couldn't come together.
Many want Lomachenko to step up in competition and he's doing that with Marriaga, even if it wasn't his first choice of fight. Marriaga is still one of the more dangerous opponents out there for Lomachenko, as he's had some impressive showings. In a unanimous-decision loss to Oscar Valdez, Marriaga looked good, and has some quality wins in recent bouts


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Market Overview
Safe haven plays have been performing well after weaker US data, more political woes for Trump and this morning's disappointment in Chinese PMIs. With sluggish US growth in the first half of the year, lower US employment costs and a constant stream of concerning newsflow out of the White House, traders have moves back into US Treasuries. This has pulled yields lower again on Monday morning and any intraday rebounds on the US dollar are likely to be seen as another chance to sell. With Donald Trump's Chief of Staff leaving his job being the latest stumbling block for a stumbling President, in addition to the news that Russia has expelled 755 US diplomats, the safe haven plays are benefiting. Despite slight dips this morning, gold and the yen continue to strengthen whilst the negative trends on the Dollar Index remain in place. The move out of risk will not have been helped by the China Manufacturing PMI data overnight which missed estimates and dropped back slightly. Although both services and manufacturing were still above 50 (signalling expansion) the data is touch sluggish. This flip-side argument is that this could induce looser monetary policy from the PBoC.
Wall Street again closed mixed on Friday with the Dow higher, but the broader S&P 500 -0.1% at 2472. Asian markets have been mixed today with the Nikkei -0.2%, although European markets are mildly higher as earnings from banking giant HSBC help to support the FTSE 100. In forex, the dollar has looked to unwind some of Friday's losses, however the yen is still outperforming. Gold is around flat, whilst the recovery in oil continues to make ground.
The big focus for today will be the Eurozone flash CPI at 1000BST. The expectation is that the headline will stay at +1.3% whilst the core CPI will stay at +1.1%. The euro and German Bunds will certainly be reactive to the data. US Pending Home Sales are at 1500BST and are expected to improve by 1.1%.
Chart of the Day – FSE 100
A sharp bearish candle posted on Friday has broken an 8 month uptrend and puts the positive outlook in jeopardy. Will the market now drop away to test the key June/July lows around 7300? An intraday breach of the support at 7358 was a 12 day low which was confirmed on the RSI, but was also a 12 day closing low too. However the concern comes with the MACD lines roiling over at neutral and the Stochastics beginning to find downside traction. Early on Monday the market has rebounded strongly (on the back of HSBC earnings) but the technicals are beginning to look more corrective. This means the reaction to the rebound will be key. another lower high and failure below 7447 would begin to put pressure back on the downside and rallies are being seen as a chance to sell. Exactly the medium to longer term implications of the waning technicals will be seen this week, with the 144 day moving average having previously been a key basis of support for the corrections since December, currently in at 7341. The hourly chart shows that the momentum has picked up again, but there is still more of a corrective slip to the market on an overall basis. Support is now in at Friday's low of 7340 with 7300 being key.
The euro continues to climb higher as the dollar remains weak. The technical outlook remains strong with the RSI continuing to hover around 70, the MACD lines rising and Stochastics solidly above 80. Corrections are being bought into as old breakouts are becoming a basis of support. Breakouts at $1.1300 and $1.1490 have been used previously and now $1.1615. The strong bull candle on Friday was another move into multi-year closing highs and there is little reason to back against the rally for now. The hourly chart shows there is a near term "buy zone" $1.1650/$1.1710, with the important support coming in initially at $1.1615.
The bulls are in control of the now six week uptrend as the market continues to pull higher. Cable has found support in the past couple of sessions above $1.3050 and is pushing higher to test the recent multi-month high at $1.3157. Momentum is strong and the RSI is holding well above 60, with the Stochastics in strong configuration and MACD lines pulling higher. The support of the uptrend currently comes in at $1.3030 and any minor retreat back to it is a chance to buy. The hourly chart shows rising moving averages and positively configured momentum that shows a market continuing to buy into weakness. There is resistance around $1.3150 from the past few sessions but a decisive breakout once more opens the upside. The next resistance is $1.3280 and then $1.3345.
Once more a consolidation candle has been followed by a strong bear candle that has encouraged a downside break in the early moves this morning. This breach of 110.60 simply continues the corrective move which should pull the market below 110.00 and towards the 50% Fibonacci retracement of 100.07/118.65 at 109.35. Intraday rallies remain a chance to sell with bullish candles incredibly rare still and negative momentum indicators configured to sell into strength. The hourly chart shows that the rally from Wednesday last week was a one off and the sellers quickly resumed control. There is now a band of overhead resistance 110.60/111.70, with the hourly RSI failing between 50/60 and hourly MACD lines failing below neutral. The June lows around 109.00 are increasingly likely to be tested.
After a degree of consolidation around $1260, the gold bulls have broken free once more and the move has re-opened the upper limits of the medium term range once more at $1295. The momentum indicators show strength but also upside potential, with the RSI in the mid-50s (tends to push to 70) and the MACD lines only just now rising above neutral, whilst the Stochastics are solidly above 80. The $1260 pivot now turns into a basis of support for intraday corrections, whilst the hourly chart shows a near term band of support $1260/$1265 for any unwinding moves. The early dip back this morning should give another chance to buy and the daily hart shows that there is room to correct within the uptrend. The next resistance above the session high of $1271is at $1280 and then the key high at $1296.
WTI Oil
And yet again the rally continues. Momentum is strong and the bulls have pushed the market above the 76.4% Fib retracement of $52.00/$42.05 at $49.65, a level which has been a historic pivot. The continued breakout is also above $50 and the reaction around this price will be key. The momentum in the rally is strong with the RSI in the mid-60s and the MACD lines accelerating into strong positive territory. The run of higher daily lows continues and this means that support to watch on a correction is in at $48.85 now. Corrections remain a chance to buy and if the market starts to pull clear of $49.65 this has openned the way for a full rally back to $52.00 which is the May high.
Dow Jones Industrial Average
The bulls remain on track as the Dow continues to push into new all-time high ground. The breakout above 21,681 opened a 210 tick upside target to 21,890 and this is now well within touching distance of today's session with the average true range at 113 ticks today. Momentum is strong with the RSI pushing towards 70 and Stochastics positively configured. Old breakouts remain supportive (the latest at 21,681) and today the bulls will look for building support above 21,756 which was Friday's low.


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Twitter Updates
- @ByErikBrady We live to die. Unfortunately, like a bug on the windshield, we just don't see it coming.... 2 days ago
- Children begin by loving their parents; then they judge them; rarely, do they forgive them. But blame is for G-d… ydrewis.wordpress.com/2017/06/28/chi… 3 months ago
- Imagination was given to man to compensate for what he is not; a sense of humor to console him for what he is. We… ydrewis.wordpress.com/2017/06/07/ima… 4 months ago
- Just as there are doctors who help people who have done bad things, there are lawyers who defend bad people. People… ydrewis.wordpress.com/2017/05/18/jus… 5 months ago
- Mother's Day can be a torment, especially if your mother's been dead less than a year… Men are what their mothers… ydrewis.wordpress.com/2017/05/11/mot… 5 months ago
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- Children begin by loving their parents; then they judge them; rarely, do they forgive them. But blame is for G-d and the small children we must keep from going insane. Leanne discovered she couldn't have them. And I had married into insanity. Indeed, mothers may be fonder than fathers of their children because they are more certain they are their own…
- Imagination was given to man to compensate for what he is not; a sense of humor to console him for what he is. We don't lose our sense of humor because we get old. We get old because we lose our sense of humor. The problem with humor is often that people you use it on aren't in a very good mood. And Joey G was cute when he was being humorless…
- Just as there are doctors who help people who have done bad things, there are lawyers who defend bad people. People do not win fights, lawyers do. Everyone thinks defense lawyers must believe their clients are innocent, but that's seldom true. Most of the time they are guilty as O.J… yet Trev also got his client off — mostly…
- Mother's Day can be a torment, especially if your mother's been dead less than a year… Men are what their mothers made them. And my mother was slightly insane. She told us baked potato skins were bad for us – so she could devour ours. Hmm… I write a mental letter to my mother every day… and apparently so does my brother…
- Any jackass can kick down a barn, but it takes a good carpenter to build one up. In the spring a young man's fancy lightly turns to love… but us 'old men' turn to painting the house. I may not be perfect, but I am avid – about not giving a damn. After all, as I told my mammoth, cigar-puffing, Italian landlord: Look what 'youse' guys did to that last Jewish carpenter…
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Monthly Archives: May 2011
Even with our haircuts in America most of us don't get what we ask for and pay for, we get clipped the way some beautiful woman decides she wants us to look.
Few of us get what we want. The rest of us get what comes. And I got Gary, this handsome Italian cop from South Philly (now that's redundant) deciding I should come with him and get my bushy mangle of … Continue reading
Posted in Uncategorized Tagged altar boy, Asian American, beauty, bin Laden, catholic, coif, computer nerd, congressman, Damn Yankees, geek, hair, haircut, Halloween, Italian, Iwo Jima, locks, Lola, maestro, mirror, mirrors, motorcycle, narcissist, narcissistic, New York, Philadelphia, pretty, red dress, salon, Secretariat, smart, South Philadelphia, South Philly, split ends, stallionsHarley Davidson, styled, tip, tresses, vain, wife, writer, xrays Leave a comment
Why is it that a little man with a plan can't get a loan, but we give Wall Street, oil thieves and foreign potentates more money than they can bury in offshore accounts?…. Because a man with a plan is considered a crank – until his idea succeeds.
At one booth in one of the many festivals and regattas swelling the city last weekend fat folks and thin folks and folks who might as well be naked seeing it didn't really make much difference, all gathered in large … Continue reading
Posted in Uncategorized Tagged Afghanistan, America, athlete, China, cigars, DROP fund, drugs, entrepreneurs, hand rolled, Havana, healthcare, Hollywood, IMF, International Monetary Fund, Iraq, Irishman, ItalianFestival, lawyers, New York, oil companies, Pennsylvania, Philadelphia, politicians, primary election, South Philadelphia, starlet, steroid, tobacco leafs, Vietnam, Wal-Mart Leave a comment
Most everything is a lie. But so what? We've got to believe in something — as long as it makes us happy…. Now ain't that the truth.
A 'happy' couple was visiting me one night. They wondered if I had brought home any of that great vodka from Russia where I was mostly working and living at the time. They chirped like mynah birds as they extolled … Continue reading
Posted in Uncategorized Tagged 1812, aficionado, aperitif, bourbon, caviar, Chianti, China, corn, cyrillic, czar, DeBeers, diamonds, ersatz fur, Europe, happiness, happy, inalienable right, Italy, Japan, King Kong, Medal of Valor, Moscow, Mother Nature, Motherland, Mt.Everest, nectar, Nikolai Vodka, Patton, pigs, posthumously, potato, Rembrandt, Rubicon, Russia, Russians, St Petersburg, Stolichnaya, Summer Palace, tank, Tchaikovsky, tsunami, viagra, vodka Leave a comment
I hate that my cruel, unfair, brutal, mean father was obviously correct about how to raise children. Afterall, look at my brother……and me
One afternoon, many full moons ago, my older brother telephoned our dear ol' Pappy. Brian wanted to impart, to our my-way-or-the-highway father, that June, his wife, was less than nine months away from bequeathing the in-laws its first grandchild. More … Continue reading
Posted in Uncategorized Tagged ADC, Agent Orange, bin Laden, bourbon, brother, Bush, capitalism, child, Colorado, corn cob pipe, course, DHS, Dick, doctor, eureka, family, father, floats, Freud, gulag, hated, heaven, hell, humor, interventional cardiologist, Jewish, legislation, Ludlow, marriage, miners, National Guard, nose, orphan, paarenting, pappy, parent, parents, Philadelphia Bulletin, Pinocchio, psycho analyzed, River city, sausage, scum, Sodomites, split infinitives, stalag, stick shift, swinging bunt, turbid, Vietnam, wife, WWII Leave a comment
Anyone who has been married with kids knows that we all torture one another. It comes naturally. Hell, just drive down the highway without your finger talking.
When I was growing up, there were two things that were unpopular in my house: One was me. The other was my trumpet. And, by golly, I may not have been any Harry James, or Satchmo or even Al Hirt. … Continue reading
Posted in Uncategorized Tagged Al Hirt, amoebas, beer, bin, black sites, brother, CIA, Darfur, Davy Jones' locker, Gabriel, Harry James, helicopters, human, inhuman, IRS, Japanese, jingoistic, Laden, lesbian, lesbian lovers, lessons of war, Mack Truck, man in the iron mask, Mata Hari, National Security Council, Noriega, Osama bin laden, Panama, Philippines, post traumatic stress, redundacy of history, righteous, righteous country, Satchmo, Tommy Vietor, torture, torture is wrong, touchdown, trumpet, U.S.Attorney General, Viet Cong, violin, water torture, weed Leave a comment
When it comes to unions, Republicans and Democrats everyone seems to forget: Don't use a hatchet to remove a fly from a friend's forehead.
So tell me who is lying. The unions stipulate that at least 12 workers a day across the country are being killed because the government isn't funding OSHA to check on all the dangerous working conditions, especially for immigrant laborers … Continue reading
Posted in Uncategorized Tagged Afghanistan, alice, Blackhawk, Blackhawk down, Blinton, capitalism, Chinese, Democrat, Desert One, fools, healthcare, Home Depot, immigrant, kosher, laborers, madness, Monica, orthodox, Osama bin laden, OSHA, Republicans, rich, truth, unions, Wal-Mart Leave a comment


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The staff is very helpful and friendly. We held a family reunion near this hotel. We had a lot of children. The staff printed off word search and activities for the kids! The pool is small but it was great to keep an eye on...More
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"Higher floors are quieter, but can not be selected using the H.I. phone app."
"get a room on the opposite side of I-84, if available and not sold out."