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Archive for March, 2011
Really? Hydrogen peroxide for yeast infection
When you're learning about something new, it's easy to feel overwhelmed by the sheer amount of relevant information available. This informative article should help you focus on the central points.
Whether you believe it or not, the use of hydrogen peroxide for yeast infection treatment has been proven to be true and quite effective. Vaginal yeast infection is among the more common conditions that women experience. In fact, according to statistics, about 75% of all women will be affected by the infection at some point. It may already be considered as an ordinary condition so don't be alarmed. You're not the only one who has the infection and certainly you will not be the last one.
Microorganisms that live on human skin and in the digestive track are the primary cause of yeast infection. Called Candida albicans, these microorganisms are naturally occurring and lives on the skin and inside your vagina. They are usually in small numbers, however some stimuli would trigger an overgrowth of Candida causeing the body to react resulting to an infection accompanied by itching, redness, irritations, and white or yellowish discharge.
Vaginal yeast infection is highly treatable so you don't have to be overly concern. There are already a number of commercially available drugs which are quite effective in treating yeast infection. Home remedies also are quite good if you know what you're doing. Hydrogen peroxide in particular is an easy home made solution to your yeast infection problems.
Just like Candida albicans hydrogen peroxide is already present in the vaginal areas. It's already there working its best in putting the yeast infectious microorganism at bay. However, due to certain conditions, the environment in the area becomes suddenly favorable to the growth of Candida. Hydrogen peroxide generally helps eliminate bacteria and fungal infections. It is a natural enemy of Candida and thus can be utilized fully as a treatment procedure for the infection.
Sometimes the most important aspects of a subject are not immediately obvious. Keep reading to get the complete picture.
Since our body produces only very little hydrogen peroxide, you can help by purchasing commercially available ones in the local supermarket or in your neighborhood pharmacy. What you can do is mix a teaspoon of ordinary 3% hydrogen peroxide to a cup of water and apply it on the affected areas. Because hydrogen peroxide is produced naturally by the body, the chemical does not harm your vagina or the general health of your body.
What makes hydrogen peroxide very effective against Candida is the fact that peroxides breaks down into water and oxygen upon application. The oxygen helps the chemical application kill the fungal yeast infection. You need to be careful, however, when creating your own mix of hydrogen peroxide treatment solution. Too much hydrogen peroxide in the solution can cause a burning sensation in the affected areas. This burning sensation may likely to occur especially if the area has been inflamed or the infection is already quite serious.
A teaspoon of hydrogen peroxide on a cup of water seems to be an okay and safe mix. So far no bad reactions or complaints have been noted against this mixture of hydrogen peroxide and water for treating yeast infection.
You need to understand, however, the using the mixture of hydrogen peroxide and water is not meant to be a sole treatment solution for yeast infection. Natural remedies like this one is more effective when combined with other forms of treatment like proper diet and the use of other treatment techniques like the use of garlic paste, yogurt, and cider vinegar and water mix.
Other times, the condition might have reached an already critical stage that hydrogen peroxide for yeast infection treatment is no longer advisable. You need to consult a doctor and employ several other methods of treatment to cure your yeast infections.
Knowing enough about Yeast Infection to make solid, informed choices cuts down on the fear factor. If you apply what you've just learned about Yeast Infection, you should have nothing to worry about.
About the Author
By Anders Eriksson, feel free to visit his top ranked GVO affiliate site: GVO
Learn To Draw Fairies
The following article lists some simple, informative tips that will help you have a better experience with Learn To Draw.
Fairies are considered to be very whimsical and magical. They are usually depicted to be beautiful, delicate and mysterious. They also bring an aura with them that makes you feel as if you were in some enchanted forest. Although they are mythical creatures, a lot of artists love drawing them. If you want to learn to draw fairies, here are some things that you should consider and try.
Your Fairy Your Rules
Since fairies are mythical creatures, there is no fixed parameters in which you would be limited to in drawing them. All those beautiful fairy pictures, drawings and paintings you have seen are all products of their respective artists' imagination. This is why, as an artist that wants to draw fairies, you would have all the power to do so.
All you need to do is imagine. Create a vision of your own fairy in your mind and this would be your guide. If you want your fairy to be a boy or girl, it all depends on you. However, for starters it is recommended that you start out with a female fairy, since this is the common.
The Traditional Fairy Look
Most of the time, fairies are depicted to be women in small sizes that have wings. They are also usually beautiful. They also glow at times or has a very colourful aura. They have the ability to fly. Their wings are most of the time depicted to be similar to a dragonfly's, which is transparent and slender. However, in some other renditions, fairies are seen to have butterfly like colourful wings that matches their colourful clothes. Of course, there is also the ever present magic wand.
Is everything making sense so far? If not, I'm sure that with just a little more reading, all the facts will fall into place.
Basic Body Parts
Fairies have two major body parts. One would be the human like body, which is generally similar to a human's figure. The second would be the wings. In some cases, artists add a little twist with their fairies. They do this by giving them pointed ears. Some would even make the head shape a little pointed too. It still depends on you whichever style you want to go for.
So basically, you would just have to draw a woman. Then, make her whimsical by adding a pair of wigs on her back. For the hair, you could either give her extremely long hair or extremely short hair with a pointed head. The hair color could also vary depending on your imagination. Almond shaped eyes are common among fairies too.
Clothing And Accessories
For clothing, your fairy can wear something that is close to nature, especially if the type of fairy that you want to draw is the kind that lives in the forest. Most fairies appear to be wearing a miniskirt that has asymmetrical edges. Their long slender legs are also prominent with their clothing. For accessories, you can draw a flower garland around their head of around their waist in a way that it would look like a belt hanging loosely. You can also add a pouch to their belt, just like the one where Tinkerbell keeps her fairy dust.
The Environment
For your fairy to appear more magical you should draw her in her environment. Again, this would depend on you. She can be playing in a pond, beside a flower or sitting at a rock. Whatever the environment is, make it appear magical and out of this world.
I hope that reading the above information was both enjoyable and educational for you. Your learning process should be ongoing–the more you understand about any subject, the more you will be able to share with others.
About the Author
By Anders Eriksson, feel free to visit his top ranked GVO affiliate site: GVO
Wine Gift Baskets: Great Wine Gift Ideas for Any Occasion
When it comes to giving gifts, you can never go wrong with wine gift baskets. Why? Well for starters, wine gift baskets are elegant and they are full of goodies that the recipient of the gift will surely love. Almost everyone loves wine and by giving them a wine gift basket, you can be sure that your gift will truly be appreciated and it will also be a gift that they won't soon forget.
There are so many wine gift basket ideas that you can apply. For starters, you can try making one from scratch and give it a personal touch or you can purchase a ready-made one that is perfect for the occasion.
You need to remember that choosing the wine basket is one thing. However, choosing the wine that you will include in the basket is another. Depending on the wine you choose, the entire wine basket may turn out to be quite expensive especially if you don't know anything about wines.
Today, there are places where you will be able to go to get the full package. This particular option is perfect for people who don't know anything about wines and wine gift baskets and for people who don't want to put their own wine gift basket together from scratch.
The best time to learn about Wine Gift Baskets is before you're in the thick of things. Wise readers will keep reading to earn some valuable Wine Gift Baskets experience while it's still free.
There are wineries today that offer wine gift baskets that are ready to go. And, you will find that you will have a wide selection to choose from. There are wine gift baskets for all sorts of special occasions, such as birthdays, anniversaries, weddings, baby showers, and even for simple occasions, such as by conveying special messages, and get well wishes, as well as congratulating someone for a job well done.
There are some specialty wine gift basket shops that offer more expensive options. These are perfect for people who are planning to give a wine gift basket to someone who is a wine connoisseur and this is also perfect as a gift for your boss on his or her special day.
When choosing a wine gift basket, make sure that the wine included is a mid range wine. Never go for cheap bottles of wine, especially if you are going to give it to a wine connoisseur or expert. This will only lead to embarrassment in your part as well as on the part of the person receiving the gift basket.
You may need to spend some money on the bottle of wine itself but it will surely be worth it. To make it even more special, include two wine glasses in the basket alone with a wine bottle opener as well as other goodies that goes great with wine, such as cheese and chocolates.
These are the things that you need to think about if you are going to give a wine gift basket to someone. By choosing the right wine and the right basket along with the right goodies to be included in the basket, you can be sure that you will be able to give that special person a gift that they won't soon forget.
When word gets around about your command of Wine Gift Baskets facts, others who need to know about Wine Gift Baskets will start to actively seek you out.
About the Author
By Anders Eriksson, proud owner of this top ranked web hosting reseller site: GVO
Getting it right, diagnosing yeast infection
Knowing what you have is always part of the battle. In case of medical conditions it is highly important to get the right diagnosis to get receive the proper treatments. In some cases, a wrong assessment of a condition or illness will result to more complications and can even lead to death. The same is true with diagnosing yeast infection. More often than not, people will not consult their doctors because they believe that what they have read on the internet suffice to make an accurate diagnosis of the condition that they have. Unfortunately, that is not always the case.
Yeast infection has been one of the more common infections that are often misdiagnosed. Thinking that what they have is yeast infection, women will often buy and apply various treatments in their attempt to cure a condition that they do not have in the first place. So instead of being cured of a simple condition, these women will end up with a more complicated condition requiring full treatment procedures. They will then have to spend twice the time and twice the money for their treatment. It's totally a waste.
Why does this keep happening? There are a number of reasons really but one of them is the fact that we think we're better than the doctors we come to for help. Some of us think that we are paying these doctors too much for simple diagnosis that we can do ourselves. Or we think we are paying too much for the medicine that they prescribe.
Other times we find our selves in a position as experts in a filed few, if there's one at all, of us really know about. Just because we have experienced having an itch or infection once or twice we think that we already know everything, from causes to treatments. When faced with an itching predicament, first thing you should do is wash the area with soap and warm water. This way you can eliminate some of the other causes of itchiness in the affected area.
It seems like new information is discovered about something every day. And the topic of Yeast Infection is no exception. Keep reading to get more fresh news about Yeast Infection.
Now in terms of cleaning the affected region, one should avoid using various products like douches and hygiene washes since these can easily irritate more the vaginal area. Also, keep in mind that antibiotics can aggravate yeast infection so it is recommended that one should not take any antibiotic pills when one has yeast infection. In fact, even taking a single antibiotic pill can worsen the infection.
Also, it is best not to panic in situations that you think you have yeast infection. Sometimes the whitish discharge is just part of your menstrual cycle. The same goes with pain and discomfort. However, if you are feeling pain that seems to quite unusual, then consulting your doctor would be the best action you should take.
Recurring infections should also be looked at by your doctor. If despite several medications, the infection keeps coming back bringing with it the other symptoms of the condition, then checking with your doctor is an absolute necessity. There may be some underlying conditions behind which you failed to notice or failed to consider.
Diagnosing yeast infection should not be done by amateurs like you and me. Getting a right diagnosis is imperative to have a treatment schedule that would efficiently help you solve your existing problems.
There's no doubt that the topic of Yeast Infection can be fascinating. If you still have unanswered questions about Yeast Infection, you may find what you're looking for in the next article.
About the Author
By Anders Eriksson, proud owner of this top ranked web hosting reseller site: GVO
Learn To Draw Animals
The following article presents the very latest information on Learn To Draw. If you have a particular interest in Learn To Draw, then this informative article is required reading.
Animals are one of the most common things that you can see, regardless of where you come from. Due to this, they have been a favourite subject to be drawn by artists of all kind. This is very evident with the accumulated works throughout history that showcased various animals from all over the world coming from different periods in time. Thus, animal drawing is one of the most popular kinds of art.
A Complicated Art
If you want to learn to draw animals, you should understand that this is a complicated kind of art. Remember that your subject here is a living being. In fact, the complexity of this form of art is comparable to how hard drawing a human figure is. You do not only have to depict the form of the animal but even capture its essence too. However, the problem here is that unlike people, you cannot really tell an animal to sit still forever until you finish your drawing. Thus, more imagination is needed.
Here are some of the other things that you should know in drawing animals.
Be Creative Because They Are Unique!
It is important for you to become extra creative when drawing an animal. This is because they are not the same. Each one of them is unique. Thus, you have to approach each in a different manner. This difference of approach is important in all aspects.
For instance, the way you'd approach them physically can be different. You can watch them from afar without being noticed or have a personal close-up encounter or even a one-on-one play session with them. Various ways of encountering animals can have a very obvious on the outcome of your drawing.
It seems like new information is discovered about something every day. And the topic of Learn To Draw is no exception. Keep reading to get more fresh news about Learn To Draw.
The way you draw an animal can be different too. They can be drawn in various ways. You can choose to depict them as cartoons. In fact they can even come out as a mere line or swirl. You can also opt for a much realistic portrait. It all depends on you.
Observe First Then Draw!
Before you can start drawing, it is imperative that you first observe your subject. It is best to see them in their natural environment. If you can't find a live encounter then you can always opt for videos. You can also look at photos or books. As you do this, you would find that there are plenty of styles that one kind of animal could be drawn. Although it is possible to master a lot of styles, it is still best that you concentrate on one style and make this your niche.
To find your niche, gather a variety of materials. By seeing animals in their different forms, you are now being exposed to their basic shapes. If you want to know precisely the basic shapes of your chosen animal, the best way to do this is by photocopying its picture. Once you have a copy, start drawing out basic shapes which you see. Observe how its body's outer lines manage to bring all these shapes together and form the subject.
Start Out With The Basics!
It is best that you start basic. After drawing the basic shapes, you could then build on it by the use of layers. Start adding details by shading and knowing important animal muscles that have become your subject's trademark. Keep on practicing and you'll be able to perfect your drawing in no time!
About the Author
By Anders Eriksson, feel free to visit his top ranked GVO affiliate site: GVO
Learn To Draw The Human Figure
Imagine the next time you join a discussion about Learn To Draw. When you start sharing the fascinating Learn To Draw facts below, your friends will be absolutely amazed.
The human figure is considered to be one of the most difficult subjects to draw. One of the most commonly encountered problem of aspiring artists would be drawing it in proportion. So, if you want to learn to draw the human figure in the right proportion, here are some basic steps in doing so.
What you'll need would be paper, pencil, a good eraser, ruler, and one good dose of imagination! Once you have all these, you are ready to start drawing.
Start With The Head
One of the easiest portion to start with would be the head. Draw the head. Create an oval for the head. This is because most heads are said to be oval in shape. Place this oval at the top portion of your paper. Try not to put too much pressure with your pencil stroke just yet. Draw lightly, and consider this to be a sketch.
Get The Body Size
Use your ruler to determine the body size. Get the measurement of the rest of your subject's body. The ideal measurement can be taken from the principle that the human head's size is approximately 1/7 of the total body height. So, measure the height of the oval that you just drew. Compute for the height of six more oval heads, and you would get the height of your body. It is best that you put marks on your paper so that it would be easy for you to see the measurement marks.
Hopefully the information presented so far has been applicable. You might also want to consider the following:
Start Having Some Figure
Once you've determined the height, you can now start giving your drawing some figure. Start by drawing the hips. Generally, you can find the widest portion of the hips located halfway down of the body. Draw a basic trapezoid shape. Put the widest or the base line of your trapezoid approximately at the position where the hips and waist should be, according to your measurements. The normal space starting from the hips up to the waist would be approximately 2/3 of the head's length.
Add The Lower Extremities And The Upper Extremities
Add more form by drawing the knees and feet. Your figure's knees should be halfway in between the feet and hips. Obviously, the feet would be the bottom part of the body. Create 2 small circles for the knees, place 2 small ellipses for the feet.
Now draw the upper extremities of the body by adding the shoulders, arms, elbows, and hands. Generally, the elbows should fall at waist level. The shoulders should be just right below the bottom of the head on both sides. Just like with knees, you can draw small circles for the elbows. To put the shoulders, draw two more heads lightly. Place 1 on each side of the head. You can draw circles to for the shoulders below each imaginary head. For the hands, they usually fall halfway down your figure's thighs. Create small ellipsis for the hands.
Get Connected!
Now, it's time for you to connect the different body parts that you drew. Use ellipses so that you can connect the body part. As soon as this is done, you could start adding some shading and details. Add some clothing and some other essentials. Do not forget to get a light source so that you'd know where the shadows should be placed.
About the Author
By Anders Eriksson, proud owner of this top ranked web hosting reseller site: GVO
Grooming Your Pet Rabbit
Like cats rabbits pride themselves on cleanliness, that is why it is so easy to teach them to use the litter box. They spend several hours a day keeping grooming themselves, maintaining the quality of their skin and coat. Just because your pet rabbit spends a large amount of its day grooming itself that you are absolved from pet grooming. Rabbits, like all pets rely on their owners to set a grooming plan and then stick with it.
One of the most important things to do is to brush your rabbit at least once a day. The time you spend brushing your pet bunny will go along way in establishing a lifelong bond. The health benefits of brushing your pet rabbit include the removal of any dead hairs that your rabbit might other wise swallow. Once swallowed these hairs could form a hairball that can compromise your rabbit's digestive system. If a hairball creates a blockage, your pet rabbit will have to go to the veterinarian where the resulting surgery could cost several hundred dollars. When you are brushing your rabbit bear in mind that the average rabbit sheds its old coat approximately every three months, during this period of time increase the frequency of your brushing, and prepare to vacuum your house twice as often, you'll be amazed at how much hair comes off the tiny body. Rabbits have very delicate skin so look for a brush that has soft bristles and wont tear your pet bunnies skin. Some rabbit owners prefer a plastic wide toothed comb for their grooming.
See how much you can learn about Pet Grooming when you take a little time to read a well-researched article? Don't miss out on the rest of this great information.
When properly groomed and cared for long haired rabbits, like Angora's, are gorgeous. The problem is keeping all that hair combed, clean, and free of mats is quite a chore. If a rabbit owner isn't showing their long haired rabbit, they typically trim the hair so that it is only one inch long. This help keep the rabbit's coat clean and free of mats but also keeps the rabbit more comfortable during the hot summer months. If you have never trimmed a rabbit's long hair before, you might want to take your rabbit to a professional pet groomer and watch how it's done before you try it yourself. When using the clippers or scissors, be very careful that you don't damage the rabbits skin.
If you find a spot where the rabbit's hair has become so tangled that it has turned into a mat, refrain from using a pair of scissors to remove the mat. It is better if you use a comb and patiently work the mat loose, a few hairs at a time. If you find that this is to trying on your patience, you might want to take your pet rabbit to a professional pet groomer and have them remove the mats with a pair of electric clippers.
Every few weeks, check and make sure that your rabbit's nails haven't gotten to long. If you think they are looking long, or notice that they are starting to catch on your carpeting it is time to trim them. If you have never done this before take your rabbit to a pet groomer or veterinarian and watch how it is done first. If you are comfortable on your own be careful that you don't trim the nails so short that they bleed and your rabbit is to uncomfortable to move around.
Don't limit yourself by refusing to learn the details about Pet Grooming. The more you know, the easier it will be to focus on what's important.
About the Author
By Anders Eriksson, feel free to visit his top ranked GVO affiliate site: GVO
The History of Pet Grooming
The best course of action to take sometimes isn't clear until you've listed and considered your alternatives. The following paragraphs should help clue you in to what the experts think is significant.
Pet grooming is a common practice in our society. People don't even think twice of spending hundreds of dollars having their pets groomed and bathed by a pet grooming professional. It is such a common connivance that many people forget the history of what made this profession what it is. If you look at the paintings of the Elizabethan era you will see pets portrayed in paintings that are well groomed and clean. Who did the grooming? Was grooming a pet an actual profession back then? Was the local barber responsible for pet care as well as cutting hair and medical responsibilities? At the local Lord or Lady have their hair cut alongside their pet There are actually paintings from that era that depict a dog being sheared while sitting on ladies lap.
As breeds became popular in the 17th century, poodles inFrance received recognition for being the dog of the court. This entry also records that professional grooming parlors were open for dogs. In 1879 Shaw wrote a book called The Book of the Dog. This book made references concerning dog grooming in England and gave helpful hints of how to clean, groom, and keep your dog healthy.
It seems like new information is discovered about something every day. And the topic of Pet Grooming is no exception. Keep reading to get more fresh news about Pet Grooming.
As animals became more a part of society, pets and work animals became more important dictated on how they looked.. The animals were groomed, either by their owners or professional pet groomers. Animals such as horses were groomed by stable boys or their owners as a kind of the embellishing of the owners taste and wealth. As animal labor was replaced by automobiles and machines, the grooming of the animal took on a more personal reflection of the owner, particularly in high society. A well groomed and clean animal was a sign of wealth and prosperity.
In today's perception of pet grooming, the animal is considered part of the family or something to show as a source of pride. Organizations such as 4H incorporate pet grooming into their program so that not only the health of the animals is displayed but also the owner's attention to the animal. Special events are organized to present current animal grooming techniques and the products of the pet grooming industry. Horses and other livestock are adorned during parades and rodeos making the animals a showpiece to the event. There are even pet grooming conventions that meet periodically around the United States and the world to present the newest techniques and equipment used in the pet grooming industry.
There are thousands of web pages dedicated to the art and fashion of grooming and as with many long-standing professions a rich history of pet grooming dictates pride and self achievement in the professional. So next time you visit your local pet grooming expert, remember that they are not just a service, but they have been backed with centuries of expertise, refinement, and dedication to the art. The next time you drop off your animal to a pet grooming expert, remember your animal will be taken care of with pride and integrity.
About the Author
By Anders Eriksson, proud owner of this top ranked web hosting reseller site: GVO
Finding Yeast Infection Treatments
Yeast infection can strike at anyone at any age. It's a disease triggered by a fungus called Candida Albicans that causes the body to produce abnormal amounts of yeast. Although yeast is found everywhere in the body, too much of it can be dangerous. The most common types of yeast infection are vaginal yeast infection, thrush, diaper rash and nail bed infection. Yeast infection is a highly treatable disease that virtually goes away without any complications. There are a lot of available yeast infection treatments for each type of infection and here are some of them.
Treating Vaginal Yeast Infections
Vaginal yeast infection is easily treated with over the counter antifungal creams like Miconazole, Tioconazole, Butoconazole, or Clotrimazole which should be applied to the infected area for one to seven days. Using birth control methods like a condom or a diaphragm is not advisable during this period. For pregnant women it's best to consult a physician for a remedy.
To prevent vaginal yeast infection from occurring, avoid using douches, feminine sprays, scented toilet paper and deodorant tampons. It is also best to wear cotton underwear and avoid wearing tight pants or panty hoses. When wearing a wet suit or a bathing suit, it is recommended to change out of it right away.
Treating Thrush
The yeast infection of the mouth called thrush can be treated with medicated mouthwashes or lozenges that dissolve in the mouth. A recommended mouthwash to use should contain nystatin that can kill the yeast fungus.
The best prevention for thrush is maintaining good oral hygiene by brushing the teeth at least twice a day, flossing at least once a day and using a mouthwash. For those using dentures, clean them thoroughly before using it. For breastfeeding mothers, it's best to have a breast check up for yeast infections.
You may not consider everything you just read to be crucial information about Yeast Infection. But don't be surprised if you find yourself recalling and using this very information in the next few days.
Treating Diaper Rashes
Diaper rashes caused by yeast infection can be treated with over the counter antifungal creams that contain nystatin or through supplements like acidophilus.
Protecting infants or adults from diaper rashes means keeping the covered area clean at all times. It also helps to allow the skin to breathe by not using a diaper at least an hour a day. It's also recommended to use cotton diapers more often than the disposable ones since cotton allows the skin to breathe more easily.
Treating Nail Bed Infections
Yeast infections of the nail bed are more difficult to treat. A prescription for an antifungal pill is needed but it can also be treated with antifungal laquer which is only proven to work in mild to moderate infections when the nails have not yet been infected by the fungus.
To prevent this infection from occurring and recurring is to regularly keep the skin dry and clean. It is also advised to avoid contact with anyone who is infected with this disease. Wearing gloves when gardening also lowers the chance of nail bed yeast infection from occurring. Another way to go about this is to avoid sharing personal items such as nail clippers or socks.
Yeast infections and its different types are highly treatable and preventable diseases which should last an average of one to two weeks and any time exceeding this means a trip to the doctor. Information on yeast infection treatments and preventions are available through online medical journals and medical sites but for highly advanced states of yeast infection it's always best to consult a physician.
Of course, it's impossible to put everything about Yeast Infection into just one article. But you can't deny that you've just added to your understanding about Yeast Infection, and that's time well spent.
About the Author
By Anders Eriksson, feel free to visit his top ranked GVO affiliate site: GVO
Wine and Cheese: The Perfect Combination for Your Wine Gift Basket
Giving gifts is already a part of human culture. Wherever you go, you will find people giving gifts to family, friends, and loved ones during special occasions, such as birthdays and anniversaries. However, no matter how old this tradition is, people still has a problem about gift giving, which is deciding what type of gift to give a certain person.
Surely, as the person giving the gift, you want your gift to be one that the receiver will truly appreciate and remember for a very long time. You want to give something that they will be able to use and also have fun with at the same time.
If this is the type of gift you want to give someone close to you, then you should definitely consider giving them a wine gift basket. Why?
For starters, you can never go wrong with a wine gift basket. It is the perfect gift for almost every occasion and it will also enable you to send the message you want to give the person receiving the gift.
Wine gift baskets are very elegant and it also screams out sophistication. It's a very tasteful gift that the receiver will truly appreciate. In addition to that, you will be able to give it to almost any type of person. Whether it may be your boss, your co-worker, your significant other, or a family member, wine gift baskets will do its job in pleasing the receiver.
Aside from wine, you have to remember that a wine gift basket should contain other types of goodies. So, what goes great with wine?
The information about Wine Gift Baskets presented here will do one of two things: either it will reinforce what you know about Wine Gift Baskets or it will teach you something new. Both are good outcomes.
Well, you can consider including fine cheese in the wine gift basket. You have to remember that wine and cheese go great together. They are perfectly matched. In fact, they go like peanut butter and jelly.
If the person you are giving the wine gift basket to enjoys wine, then adding slices of assorted fine cheeses will definitely make the gift basket a lot more special. However, you have to keep in mind that whenever you are going to give away wine and cheese, make sure that the wine you choose is perfect for cheese.
The correct cheese with the right brand of wine is very important. The rule here is to get a get cheese from the same region or country where the wine was made. Acidic cheese goes will with acidic wines.
For example, blue cheese goes well with Cabernet Sauvignon, Zinfandel, and Shiraz. Chardonnay and Pinot Noir go well with the Brie variety of cheese. If you are going for a bottle of Reisling, Shiraz or Merlot, cheddar cheese is a great choice for it. For Swiss cheese, you should go for a bottle of Cabernet Sauvignon, Pinot Noir, or Sauvignon Blanc.
These are the examples of great wine and cheese pairs. As you can see, by choosing the right cheese for the bottle of wine you have in your wine gift basket, you can be sure that the receiver will truly enjoy your tasteful gift.
With the proper combination of cheese and wine, you will see that both will complement each other's flavor, which the person drinking the wine and eating the cheese will truly appreciate and remember for a very long time.
As your knowledge about Wine Gift Baskets continues to grow, you will begin to see how Wine Gift Baskets fits into the overall scheme of things. Knowing how something relates to the rest of the world is important too.
About the Author
By Anders Eriksson, feel free to visit his top ranked GVO affiliate site: GVO


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Monday, 1 August, is the first day for South Africans who applied for special votes to cast their votes for the 2016 Local Government Elections.
Maureen Valsecchi of Dainfern in Johannesburg says this is a golden opportunity for all eligible voters to exercise their democratic rights and that those who don't vote have no say and can't complain about not getting services from government.
Valsecchi who exercised her right to vote alongside her husband Phillip at station at Dainfern College, in Fourway, said voting is very important because she needed to have a say in how the country is governed.
"If one does not vote, they actually have no say and cannot complain if they don't get what they want. We knew that we would not be able to vote on 3 August so we applied for special votes," she said, illustrating how seriously she takes elections.
Also See: IEC Disqualifies EFF Candidate After He Said Whites Should Be Hacked And Killed
Special voters are given until tomorrow 2nd August to exercise their right to vote while others will be voting on Wednesday, 3 August. Valsecchi said she and her husband encourage their children to vote and they are all registered to vote.
Similarly, Lidia Rauch, one of more than 50 000 people expected to cast special votes reiterated Valsecchi's view about the importance of voting by saying : "I believe that it's a democratic right I will practice. I want to see the party I voted for last back in government,"
The 27-year-old from Kloof Street in Cape Town said she chose to cast her vote at Jan Van Riebeeck Primary School because she wanted the right to choose her local government.
Rauch said it was important for every South African to vote since the event only happens every five years.
"Especially with service delivery issues you can vote out the local government who is not doing anything of benefit to your area. Local government immediately affects you, if you don't use your right to vote it's the same as saying that you don't care."
Valsecchi however says she is not convinced that youth understand the importance of election talk less of participating in it and she puts the responsibility on parents to educate their wards on the need to participate in elections not as an obligation, but as their right.
Also See: 'Watch Out For Those ANC Thieves At The Polls' – UDM
Meanwhile, the IEC spokeswoman Kate Bapela said on Monday that it is possible that the opening of some voting stations will be delayed. Though she was yet to confirm and name such places, Bapela said the voting stations she had visited in Fourways and the south of Johannesburg were all ready to go.
"I haven't received detailed feedback on where the problems are at the moment," said Bapela.
The commission received over 740 000 applications for special votes which is three times more than those received in 2011. About 44% of the applications were for home visits and 56% from voters who will vote at the various voting stations around the country.

subset: commoncrawl


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Baltimore Basilica
BBB's Master Plan for The Baltimore Basilica sets restoration goals for the oldest Roman Catholic Cathedral in the United States.
The Baltimore Basilica, designed by Benjamin Henry Latrobe and consecrated in 1821, is listed on the National Register of Historic Places. At the direction of his eminence Cardinal Keeler, BBB developed a comprehensive historic survey, a facilities assessment and a long range Master Plan for the landmark building and its prominent site on Cathedral Hill in downtown Baltimore. BBB's study sets the course for the Basilica's next 200 years, positively impacting the Parish, the historic Mount Vernon neighborhood, and the city of Baltimore at large. The Master Plan also identifies the need for additional space, including a museum and visitor's center to document and interpret the significance of the Mother Church of Catholicism in the United States, and the evolution of Catholicism in the "New World."
Construction Cost
Partner, Director of the DC Office
Associate Partner
08 May 2003The Catholic Review—Let There Be Light
01 Aug 2002Achitecture—Latrobes Salvation/Faith Restored


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NCR Corp. (NCR) Sets New 52-Week High at $34.99
NCR Corp. (NYSE:NCR)'s share price hit a new 52-week high during trading on Tuesday . The stock traded as high as $34.99 and last traded at $34.63, with a volume of 287,126 shares trading hands. The stock had previously closed at $34.39.
Several equities analysts recently commented on the stock. JPMorgan Chase & Co. boosted their price objective on shares of NCR Corp. from $37.00 to $41.00 and gave the company an "overweight" rating in a research note on Monday, August 15th. Zacks Investment Research raised shares of NCR Corp. from a "hold" rating to a "buy" rating and set a $37.00 price objective for the company in a research note on Friday, July 29th. TheStreet raised shares of NCR Corp. from a "hold" rating to a "buy" rating in a research note on Wednesday, July 27th. Finally, Wedbush boosted their price target on shares of NCR Corp. from $20.00 to $25.00 and gave the stock a "neutral" rating in a research note on Wednesday, July 27th. Four analysts have rated the stock with a hold rating and seven have issued a buy rating to the company's stock. The stock currently has a consensus rating of "Buy" and an average price target of $31.88.
The firm has a 50 day moving average price of $32.38 and a 200 day moving average price of $29.26. The firm has a market capitalization of $4.21 billion and a PE ratio of 24.24.
NCR Corp. (NYSE:NCR) last issued its quarterly earnings data on Tuesday, July 26th. The company reported $0.72 earnings per share for the quarter, topping the Thomson Reuters' consensus estimate of $0.64 by $0.08. The business earned $1.62 billion during the quarter, compared to the consensus estimate of $1.56 billion. NCR Corp.'s revenue for the quarter was up 1.0% on a year-over-year basis. During the same quarter last year, the company earned $0.66 EPS. Equities research analysts predict that NCR Corp. will post $2.95 earnings per share for the current year.
In other NCR Corp. news, EVP Andrea Ledford sold 10,000 shares of the firm's stock in a transaction that occurred on Monday, August 15th. The shares were sold at an average price of $33.40, for a total transaction of $334,000.00. Following the sale, the executive vice president now owns 98,285 shares in the company, valued at approximately $3,282,719. The transaction was disclosed in a document filed with the SEC, which is available through this hyperlink. Also, SVP Andrew S. Heyman sold 16,119 shares of the firm's stock in a transaction that occurred on Wednesday, July 20th. The stock was sold at an average price of $31.17, for a total transaction of $502,429.23. Following the sale, the senior vice president now owns 24,396 shares in the company, valued at approximately $760,423.32. The disclosure for this sale can be found here.
A hedge fund recently raised its stake in NCR Corp. stock. BNP Paribas Arbitrage SA boosted its position in NCR Corp. (NYSE:NCR) by 549.4% during the fourth quarter, according to its most recent filing with the SEC. The firm owned 209,076 shares of the company's stock after buying an additional 176,882 shares during the period. BNP Paribas Arbitrage SA owned 0.12% of NCR Corp. worth $5,114,000 at the end of the most recent quarter.
NCR Corporation is a technology company that provides products and services that enable businesses to connect, interact and transact with their customers. The Company operates through four segments: Financial Services, Retail Solutions, Hospitality and Emerging Industries. Its portfolio of self-service and assisted-service solutions serve customers in the financial services, retail, hospitality, travel and telecommunications and technology industries.
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Ronald McDonald House of Dallas © 2017 | All Rights Reserved
8687 N Central Expy, Dallas, TX 75225, United States +1.214.631.7354 firstname.lastname@example.com
The 2017 exhibit will open on November 18 and run through January 7th. Tickets will be available for purchase online in November.
All proceeds from ticket sales go to helping families at Ronald McDonald House of Dallas. Admission prices listed below:
There are multiple ways to purchase tickets to the exhibit:


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Howard College student accounts
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Learn about Howard College student accounts, and registering for elective seminars and discussion sections. As a student, your first stop should be the registrar's office, where you can pick up a free copy of both the academic calendar and course catalog, in order to find available credit classes that you may be interested in taking. Consult with an academic advisor, to ensure that your chosen classes constitute a full-time course load, and fulfills major requirements. Finally, check with your major department for assistance with prerequisites, tuition payment deadlines, and Howard College student life information. Please use the form above to request admissions info for Howard College.
Don't imagine that college is all about getting good grades and finding a job after graduation. A large part of the university growth experience is about participating in extracurricular activities such as student government, writing for the school newspaper, or joining one of the many student clubs on campus.
It isn't necessary to take a leadership position in a student organization on campus, in order to enjoy contributing to student life at your school. Sure, you have to live somewhere, but spending your freshman year in the dorms can give you the added benefit of making friends and establishing a social circle. Consider signing up for a meal plan, and gaining 10 pounds in the dining hall. Again, the purpose is to socialize and meet other students outside the classroom. Beyond consideration for Howard College student accounts, you may think about Kankakee Community College academic calendar 2017 for complementary electives to your primary course of study.
Admissions Representative
Aviation Institute of Maintenance
Prefer prior admissions experience at a private for profit college or technical school. Henderson, Campus Director of Admissions.
8 hours ago Academic Program Manager
Towson University
Prior experience in college or university Admissions and/or Academic Program Advising preferred. Participate in high school college fairs.
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Florida International University
Collaborates and assist the Admissions staff. College fairs) of assigned territories, including targeted international territories..
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Familiarity with the college admissions process. Assist families through the college selection and admissions process by determining ideal colleges for students.
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Austin Community College
Serves on high school, college, and community committees, as assigned. Resolves concerns for program participants/students, faculty, Austin Community College..
4 days ago


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Compact size
Compact size and low weight make this unit preferred solution for existing machines with limited space
Powerful strobe illumination enables successfull high accuracy code reading with small mark size on fastest machines in the world
Code marks placed on the web in an upstream process usually indicate a warning to a downstream operator. With a mark reader the marks can be automatically detected and defect map can be synchronized on spot. A mark reader is closing the loop on your defect detection and removal program.


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I tried to find information about this work, but Poolside's explanation is perfect. I've just copied their own description from soundcloud. Read it below:
This is a cover version of "Magic Table" by Spike. It was originally released in 1983, but was then re-released by Golf Channel Recordings in 2011. Our friend Ryan showed us the song cause it freaked him out that Spike's voice sounds exactly like Filip's voice. When we heard it we were blown away with the similarity and decided to do a cover just to see how similar the voices are (also cause it's a damn good song). We were happy with the result and decided to put it on our last mixtape and you can download it here for free.


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SmartPipe™ - Just Hang-it, Connect-it and Use-it!
SmartPipe™ is Kaeser's new modular aluminum piping system. This versatile piping system features lightweight materials and push-to-fit connectors that can be installed without threading, welding or sweating. There are no special tools or trades required to install SmartPipe™ - and the simple to use connecting and mounting hardware will get everything up and running in no time.
SmartPipe™ is exceptionally versatile and can be easily integrated into existing systems made from other pipe materials. It can also be modified to accommodate changing needs. The smooth, calibrated aluminum provides the best possible flow, and will not rust or corrode over time. Full bore fittings minimize pressure drop and leak-free connectors prevent costly compressed air loss.
Easy Installation, Better Air Quality Available in multiple sizes from ½ to 4 inches, SmartPipe is ideal for headers and branch lines all the way down to the point of use. Simple mounting and connecting hardware make installation a breeze. Let us show you just how smart Kaeser's SmartPipe can be!


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Bye-Bye, Barriers
By Thomas J. Bray, a staff columnist at the Detroit News. His OpinionJournal.com column appears Tuesdays.
H aving moved to Detroit in 1983, I found last year's political campaigning particularly remarkable for the dog that didn't bark: the fight over free trade.
Al Gore refused to turn his back on his record as a free trader despite the prodding of big labor. George W. Bush cited the North American Free Trade Agreement as an...


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This paper describes a method for local heating of discrete micro-liter scale liquid droplets. The droplets are covered with magnetic porous Si microparticles, and heating is achieved by application of an external alternating electromagnetic field. The magnetic porous Si microparticles consist of two layers: the top layer contains a photonic code and it is hydrophobic, with surface-grafted dodecyl moieties. The bottom layer consists of a hydrophilic Si oxide host layer that is infused with Fe3O4 nanoparticles. The amphiphilic microparticles spontaneously align at the interface of a water droplet immersed in mineral oil, allowing manipulation of the droplets by application of a magnetic field. Application of an oscillating magnetic field (338 kHz, 18A RMS current in a coil surrounding the experiment) generates heat in the superparamagnetic particles that can raise the temperature of the enclosed water droplet to >80 °C within 5 min.
A simple microfluidics application is demonstrated: combining complementary DNA strands contained in separate droplets and then thermally inducing dehybridization of the conjugate. The complementary oligonucleotides were conjugated with the cyanine dye fluorophores Cy3 and Cy5 to quantify the melting/re-binding reaction by fluorescence resonance energy transfer (FRET). The magnetic porous Si microparticles were prepared as photonic crystals, containing spectral codes that allowed the identification of the droplets by reflectivity spectroscopy. The technique demonstrates the feasibility of tagging, manipulating, and heating small volumes of liquids without the use of conventional microfluidic channel and heating systems.
Much of the work on microfluidics to date has involved production of patterned fluidic circuits consisting of microchannels.1–7 An alternative approach involves the manipulation of discrete droplets rather than continuous liquid streams.8–13 Through independent micro-manipulation of discrete droplets, complex procedures can be carried out in a manner that directly mimics traditional bench-top protocols. Because each droplet can be independently controlled, highly integrated, scalable and flexible architectures can be implemented.10 A number of techniques have been described for the actuation of droplets on solid surfaces including the use of thermocapillary effects,14 photochemical effects,15 electrochemical gradients,16 surface tension gradients,17 temperature gradients,18 air pressure,19 structured surfaces,20 dielectrophoresis,21 and electrostatic methods.8
An extension of this approach is a liquid-liquid microfluidic system for manipulating freely suspended microliter or nanoliter droplets. Only few such systems have been reported to date and new manipulation methods continue to emerge.22 Pollack et al.8 have demonstrated the electrowetting-based transport of aqueous electrolyte droplets in a silicone oil media using a two-sided open-channel planar microactuator structure. Velev et al.9,23 described a system in which water or dodecene microdroplets float freely on a surface of fluorinated oil and electric fields are applied through an array of electrodes below the surface of the oil phase to manipulate the droplets. Kotz et al.7,24 have demonstrated that asymmetric laser heating of the liquid/liquid interface between an aqueous droplet and its surrounding immiscible fluid can induce thermal Marangoni flows to move the droplet, and the utility of the method was demonstrated with a protein assay. Recently, we demonstrated the manipulation and identification of liquid droplets using amphiphilic, magnetic, one-dimensional photonic crystal chaperones, made from porous Si.25 The chaperones align themselves at the interface of an aqueous droplet immersed in an organic medium and application of an external magnetic field allows transport of the droplet. Furthermore, the optical reflectivity spectrum of the photonic crystal displays a peak that serves to identify the droplet. The coated droplets can be coalesced with other droplets under the influence of a magnetic field to perform simple microreactions.
A key requirement for many biological and chemical reactions is efficient heating of the sample. For example, the ability to perform the polymerase chain reaction (PCR) with high efficiency in microfluidic environments is critically dependent on rapid and precise heat transfer.2 Non-zonal heating is generally accomplished using a Peltier device,26 a thin film heater27 or a laboratory hotplate.28 Accurate zonal heating may be achieved through the use of complex on-chip resistive heater networks, requiring additional fabrication steps.29 The primary restriction associated with these heating methods is the thermal properties and mass of the heating block and the reaction chamber, which ultimately limit the rate at which the sample can be heated and cooled.30,31 The thermal mass problem can be eliminated by using non-contact heating methods to specifically heat the sample or the reaction medium.30,32 All the heating techniques described above have been developed for microchannel-based microfluidics and also have been implemented in droplet-based microfluidics. However, advanced droplet-based microfludics requires efficient localized heating of the individual droplets with minimal heat transfer to the surroundings.
The present paper describes a method for precise local heating of small liquid volumes using magnetic amphiphilic porous Si microparticles. The magnetic properties of the magentite particles infused within one layer of the bifunctional porous Si microparticles are utilized for heat generation by application of an alternating radio frequency (RF) electromagnetic field. The feasibility of this heating concept in performing microreactions is demonstrated by melting complementary oligonucleotides conjugated with dye molecules, while monitoring the dehybridization in real time using fluorescence resonance energy transfer (FRET).
All reagents were used as received. Aqueous HF (48%) and ethanol (99.9%) were supplied by Fisher Scientific and AAper, respectively. 1-dodecene (95%) was supplied by Sigma-Aldrich. Porous Si samples were prepared with single-crystalline highly-doped p-type Si wafers from Siltronix Corp. (0.0008 Ω-cm resistivity, <100> oriented, B-doped).
Porous Si samples were prepared by anodization of a highly-doped p-type Si wafer in ethanolic HF solution (3:1 (v/v) 48% aqueous HF:ethanol) using a platinum mesh counter-electrode. CAUTION: hydrofluoric acid is toxic and highly irritating to the skin; prompt medical attention should be obtained if the liquid contacts the skin or if the vapors are inhaled. Si wafers with an exposed area of 1.33 cm2 were contacted on the back side with a strip of aluminum foil and mounted in a Teflon etching cell. Two layers were prepared in each sample; one is referred to as the "encoding" layer and the other is the "host" layer, intended to contain magnetite nanoparticles. The "encoding" layer was prepared as a one-dimensional photonic crystal using the waveform superposition method previously described.33 Two types of samples were prepared for encoding purposes: (1) a "one peak" rugate filter was etched using a sinusoidal current waveform oscillating between 11.3 and 56.8 mA/cm2, the frequency of the waveform was 0.18 s−1, repeated for 400 s in a 3:1 (v/v) ethanolic HF solution; (2) a "three peak" sample was etched using a current waveform composed of three sine waves with frequencies of 1.5, 1.6, and 1.7 s−1 for 400 s in 3:1 (v/v) ethanolic HF solution. After etching, the sample was rinsed with ethanol several times and then dried under a stream of nitrogen. Thermal hydrosilylation was accomplished by placing the porous Si sample in liquid 1-dodecene in a Schlenk flask, degassing the liquid with three successive freeze-pump-thaw cycles, and then heating at 140 °C under nitrogen for two hours 25,34. After removal of the solution, samples were rinsed with ethanol and acetone several times and dried under a stream of nitrogen. The "host" layer (for magnetite nanoparticles) was then etched into the substrate, immediately beneath the encoding layer, using a constant current density of 303 mA/cm2 for 200 s in the same ethanolic HF etching solution. The double-layered porous Si film was then removed from the crystalline Si substrate by applying a current pulse of ca. 15 mA/cm2 in a 3.3% (v/v) ethanolic HF solution. After removal of the etching solution, the freestanding porous Si film was rinsed with ethanol and acetone several times and then dried under a stream of nitrogen.
A magnetite (Fe3O4) colloidal suspension with a mean particle size of 10 nm (measured by Transmission Electron Microscopy) was prepared using a previously described method.35 The magnetite suspension was mixed with acetone at a ratio of 1:3 (v/v). The concentration of the final magnetite suspension was approx. 1 mg/mL. The porous Si film (with its host layer side facing up) was placed in the center of an aluminum weighing dish and a strong magnet was located under the dish. A volume of 0.5 mL of the magnetite nanoparticle suspension was added on top of the film. When the solution evaporated, the magnetized film was rinsed thoroughly with ethanol and acetone several times and then dried in air. The film was then thermally oxidized at 100 °C overnight. The thermally oxidized film was fractured to micron-sized particles by ultrasonication (20 s) in acetone. The resulting magnetic porous Si microparticles were rinsed with ethanol and acetone several times and then filtered using a 100 μm filter to remove free magnetite nanoparticles and small aggregates. The magnetized porous Si particles were separated from the non-magnetized particles with a NdFeB rare earth magnet (National Imports NSN0550, 3.18 mm diameter and 25.40 mm length, grade N40). The final size of the magnetic porous Si microparticles was 150~200 μm.
Scanning electron microscope (SEM) images of the cross section of the magnetized porous Si film were obtained using a FEI Quanta 600 environmental scanning electron microscope equipped with an EDS Oxford ELX II apparatus and a four-sector back-scattered electron detector.
Magnetic properties were investigated using a Quantum Design MPMS2 SQUID magnetometer with fields up to 5000 Oe at 300 K. Measurements were performed on magnetite nanoparticles, porous Si particles, and magnetite-infused porous Si particles. The amount of magnetite nanoparticles infused into the porous Si host layer was quantified by dissolving the host matrix in an aqueous solution containing 5M NaOH and ~10% ethanol at room temperature overnight. The residue was filtered, dried, and weighed. It was assumed that the residue is composed of an agglomeration of magnetite nanoparticles. Three samples were used for the dissolution tests and the measurements were averaged. These numbers were then compared with the magnetometer results.
Interferometric reflectance spectra of encoded magnetic porous Si microparticles surrounding an aqueous droplet immersed in oil were collected using an Ocean Optics CCD S-2000 spectrometer fitted with a microscope objective lens coupled to a bifurcated fiber optic cable. A tungsten light source was focused (spot size approx. 1 mm2) onto the magnetic porous Si microparticles located at the droplet/oil interface. Reflectivity data were recorded in the wavelength range 400–1000 nm, with a spectral acquisition time of 100 ms. Both illumination of the surface and detection of the reflected light were performed along an axis coincident with a vector normal to the droplet/oil interface.
Alternating electromagnetic fields were generated by applying an alternating current to a coil with 2 turns and a cross section of ~ 70 mm2. The coils were wrapped around a Petri dish containing the aqueous droplets suspended in oil. The Petri dish had a depression in its center in order to fix the droplet in one place. A power supply (Ameritherm Nova 3) was used to apply alternating electromagnetic fields resonating at 338 kHz. Maximum power (3kW) was used for most experiments (equivalent to 18 Amps RMS through the coil). During the experiments, cooling water (15 °C) was circulated through the coil to avoid overheating of the coil. The temperature within the droplet was measured using a metallic K-type thermocouple probe, inserted immediately after the coil power was switched off.
All fluorophore-conjugated DNA oligonucleotides for the FRET assay were obtained from Integrated DNA Technologies, Inc. The sequence of the Cy3-conjugated oligonucleotide was 5′ Cy3 TGA TTC AAG CCG ACT 3′, and the Cy5-conjugated oligonucleotide was 5′ AGT CGG CTT GAA TCA Cy5 3′. The melting temperature for the pair was calculated to be 46.8°C in 50 mM NaCl.
Droplet #1 (D1) contained 0.6 nmole of Cy3-oligonucleotides in a volume of 25 μL of buffer solution and was partially covered with the "one peak"-encoded magnetic porous Si microparticles (~2 mg). Droplet #2 (D2) contained 0.6 nmole of Cy5-oligonucleotides in a volume of 25 μL of buffer and was covered with the "three peak"-encoded magnetic porous Si microparticles (~2 mg). The aqueous buffer used in the droplets contained ultra-pure water (Sigma-Aldrich) and Dulbecco's phosphate buffered saline (DPBS without calcium and magnesium, Sigma-Aldrich) with a composition of 54.4 mM NaCl, 1.1 mM KCl, 3.2 mM Na2HPO4 and 0.6 mM KH2PO4. These encoded droplets were placed in mineral oil (Sigma-Aldrich) within the experimental dish. Using a magnet, the Cy3 droplet was moved and merged with the Cy5 droplet located in the center of the experimental dish. The mixed droplet, partially covered with the magnetic porous Si microparticles (~4 mg), was heated in 0.5 mL of mineral oil with an alternating electromagnetic field (3 kW) while the photoluminescence spectrum was monitored for FRET.
The steady-state photoluminescence (PL) spectra of FRET from the droplet were obtained with an Acton 0.275 m monochromator, 500 nm-cutoff filter, and a UV-enhanced liquid nitrogen-cooled, charge-coupled device (CCD) detector (Princeton Instruments) fitted with a microscope objective lens coupled to fiber optic cable. Blue light with an emission wavelength range of 470 ± 20 nm (150 watts, Illumatool Bright Light System LT-9900, Lightools Research) was used as the excitation source and routed through two flexible fiber-optic cables. The light source and detector lens were located at a distance of ~12 cm from the top of the droplet to prevent any heat generation in the metallic materials of the detector and light source. Fluorescence measurements were obtained normal to the droplet/dish (with the two excitation sources at ~45 degrees from normal), and integrated for 2 s.
The relationship between temperature and fluorescence measured using RF field-induced heating was compared with that observed when the droplet/oil apparatus was heated with a laboratory hotplate. The experimental dish containing the FRET probe-loaded droplet described above was placed on top of a 100 mL beaker of water and heated to 60 °C. The hotplate was removed and fluorescence spectra were obtained at 1 °C intervals as the droplet cooled to 35 °C. The beaker containing 100 mL of water was used as a heat reservoir to slow the cooling rate to approximately 0.5 °C per minute. Temperature of the droplet was monitored continuously with a thermocouple probe.
The synthesis scheme followed to produce magnetic amphiphilic porous Si microparticles is outlined in Fig. 1. A multilayered porous Si dielectric mirror, referred to as the encoding layer, is first electrochemically etched into the (100) face of a single-crystal, p-type Si wafer using a temporally modulated current waveform. The variation in refractive index resulting from the modulated etch produces sharp resonance features in the optical reflectivity spectrum of the film, whose wavelengths are determined by the characteristic frequency components of the modulated current waveform.33 The film is then thermally hydrosilylated with 1-dodecene, following published procedures,25,34 to generate a chemically stable hydrophobic layer. A second porous layer, to act as a host for magnetite nanoparticles, is etched into the substrate immediately beneath the encoding layer. The etching conditions for the host layer were optimized to create relatively large and cylindrically-shaped pores to accommodate the magnetite nanoparticles with a mean size of 10 nm. The electron microscope image of a porous Si microparticle (Fig. 2a) reveals an average pore size of ~50 nm for the host layer while the pore size of the encoding layer is less than 10 nm.
After etching, the double-layered structure is removed from the Si substrate by application of a current pulse. The host layer is then infused with magnetite (Fe3O4) nanoparticles. The magnetite concentration within the porous Si particles is critical not only to allow manipulation of the droplets25 but also to allow efficient heat generation when subjected to an alternating electromagnetic field. A highly concentrated acetone dispersion of magnetite nanoparticles (mean particle diameter of 10 nm35) is infiltrated into the host layer using a strong rare-earth magnet located under the porous Si film. The solvent is then evaporated while the sample is maintained in the magnetic field. It is suggested that the magnetic field and the interfacial capillary forces generated during evaporation of the solvent36 results in enhanced infusion of the magnetic nanoparticles into the porous structure. Moreover, the surfactant tetramethyl ammonium hydroxide (TMAOH) present in the magnetite nanoparticle dispersion is often used for anisotropic etching of Si.37 Thus, the surfactant may further enhance the infusion and entrapment of the magnetite nanoparticles within the structure by slightly etching the porous Si host layer. The encoding layer is protected from chemical attack by the passivating dodecene surface modification step.
The magnetite infiltration step is followed by thermal oxidation to further trap the nanoparticles in the Si matrix.25 Thermal oxidation preferentially produces oxide on the more reactive, hydrogen-terminated host layer compared with the chemically stable, hydrosilylated encoding layer. The oxide imparts hydrophilic character to the layer, whereas the hydrosilylated side retains its hydrophobic nature, allowing the particles to align at an aqueous/organic liquid interface. The infiltration of magnetite (iron oxide) nanoparticles in the porous Si host layer is confirmed by energy dispersive X-ray spectroscopy (EDS). An elemental line scan for iron and oxygen obtained along the cross-section of a magnetic porous Si micoparticle (shown superimposed over the backscattered-electron image in Fig. 2b) reveals a sharp concentration gradient of iron and oxygen, with a maximum concentration close to the outer surface of the host layer. The thickness of the porous Si layer containing iron is approx. 10 μm. This layer is observed as a bright region in the backscattered-electron image (Fig. 2b), due to the large difference in atomic number between iron and the other constituents of the film (silicon, oxygen, carbon, hydrogen, and the boron dopant for p-type silicon). Thus, the magnetite nanoparticles are solely infused into the large pores of the host layer. The backscattered-electron image of the encoding layer displays an alternating contrast pattern, arising from the density modulation produced by the periodic current waveform used in its preparation.
The magnetic properties of the porous Si microparticles were studied at room temperature by SQUID magnetometry (Fig. 3). The magnetization curve of magnetite-infused porous Si microparticles shows superparamagnetism similar to pure magnetite nanoparticles. The saturation magnetization of the magnetic porous Si microparticles is lower than that of the pure magnetite nanoparticles due to the lower concentration of magnetite nanoparticles in the diamagnetic porous Si matrix. To test this assertion, magnetite loading within the porous Si particles was determined by dissolving the porous Si host particles in ethanolic 5 M NaOH solution and weighing the residue. This gravimetric determination yields a weight percent of magnetite nanoparticles in the microparticles of ~32 %. This result is consistent with the SQUID data, which indicate magnetite content of ~29 wt % (calculated from the magnetization saturation values, Fig. 3).
It was previously demonstrated that one-dimensional photonic crystals made from porous Si can be imparted with amphiphilic properties. These particles spontaneously assemble and orient at the interface between two immiscible fluids.34 Adding magnetite to the particles endows them with the ability to be manipulated with magnetic fields, providing a means to direct the motion of liquid droplets in microfluidics applications.25 In this study, the superparamagnetic properties of the porous Si particles are utilized not only for manipulation but also for heat generation, by application of an alternating electromagnetic field. Thus, each layer of the amphiphilic magnetic particles has its specific function and the preferential location of these layers at the interface between the aqueous droplet and a surrounding organic liquid is of utmost importance. The particles are designed so that the magnetite host layer of the particles is hydrophilic and the encoding layer is hydrophobic. As a result, the particles align at the interface of an aqueous droplet immersed in oil with their magnetic layer facing the aqueous droplet and the encoding layer facing the organic phase. This self-assembly allows identification of the droplet using the spectrum of light reflected from the encoding layer and efficient heat transfer from the magnetic layer into the droplet.
In this study, different spectral codes are used to identify each of the two droplets containing a component of the FRET probe. Droplet 1 (D1) contains Cy3-conjugated oligonucleotides and the magnetic porous Si particles surrounding this droplet are characterized by a single spectral reflectance peak (Fig. 4a). Droplet 2 (D2) contains the Cy5-labeled oligonucleotides and is coated with particles characterized by a three-peak spectral code (Fig. 4a). The droplets were half-covered with magnetic porous Si particles in order to allow detection of the fluorescence from a FRET probe contained in the droplet using a cooled CCD detector, which will be discussed in the next section. The two droplets are merged by moving droplet 1 into a shallow well containing droplet 2 by means of a small rare-earth permanent magnet (Fig. 4a). After droplet fusion, the spectral code recorded from the particles covering the mixed droplet contains four peaks, resulting from a combination of the spectrum of the particles surrounding droplet 1 (one peak) and 2 (three peaks), Fig. 4b. The spectral 'barcodes' incorporated in the magnetic porous Si microparticles allow different droplets to be interrogated and identified in an ensemble of droplets. This approach is expected to be especially useful for performing complex bioassays containing multiple distinct liquid droplets and may be further developed with programmable control on a patterned magnetic surface.38
The magnetite nanoparticles infused within the porous Si microparticles can be heated by application of a radiofrequency electromagnetic field. A discrete aqueous droplet covered with magnetic porous Si microparticles and suspended in mineral oil was heated using a 338 kHz RF field of 3kW transmitted power. Fig. 5 depicts the temperature of the droplet vs. heating time. The temperature of the droplet increases from room temperature to 65 °C within 60 s, and a maximum temperature of more than 80 °C is reached after 300 s. At temperatures greater than 80 °C, gas bubbles are observed at the surface of the aqueous droplet, suggesting that the temperature in some regions of the droplet has exceeded the boiling point of water. A bare droplet (not covered with magnetic porous Si microparticles) suspended in mineral oil exhibits a temperature increase of only 5 °C after 300 s in the RF field. Similarly, a droplet surrounded by porous Si microparticles that contain little or no magnetite show ~5 °C temperature rise after 300 s in the RF field. These control experiments confirm that the temperature increase in the droplet is largely a result of heat generated in the magnetic porous Si microparticles covering the droplet. The small temperature increase observed in the control experiments can be attributed to resistive (Joule) heating of the coil surrounding the experimental dish and weak inductive heating of water subjected to the alternating electromagnetic field.
The heat generation mechanisms available to magnetic nanoparticles has been described in detail.39–41 In the present case, the physical mechanism by which the magnetic porous Si particles are heated in the presence of the external alternating electromagnetic field is attributed to Néel relaxational losses that occur upon reorientation of the magnetic moments in the magnetite nanoparticles.40 Inductive heating of the magnetic particles via eddy currents may be neglected due to the low frequencies used.42 The amount of heat generated in magnetic materials is strongly dependent on the particle size and microstructure as well as the applied field strength and frequency.40,41,43,44 The amount of heating that can be obtained by hysteresis losses decreases dramatically as the particle radius decreases below 10 nm, whereas heating due to Néel relaxation becomes significant in this size regime.41 The ability of the porous Si host to localize high concentrations of magnetite nanoparticles while maintaining their superparamagnetic properties allows heating at relatively low fields.
Fig. 6 depicts the effect of electromagnetic field strength on the droplet heating rate. At several values of coil current, power was applied for 30 s and the droplet temperature was measured immediately afterwards. As expected, the temperature of the droplet increases as the current in the RF coil (directly related to field strength) increases. This effect is more pronounced in the lower current range, where the temperature gradient between the droplet and the surroundings is small.
The time needed to reach the steady-state temperatures reported here is somewhat longer than desired for many microfluidic applications such as thermal cycling in PCR. The slow time to reach steady state is due to heat transfer to the oil (and room air) surrounding the droplet45—factors derived from the present experimental setup, which was not optimized for rapid cycling. In addition, the thermocouple used to measure temperature in Figs 5 and and66 has a significant thermal mass relative to the droplet being probed, and so the peak temperatures measured represent somewhat of an under-estimate. Additional factors, such as the amount of magnetic porous Si particles and the volume of oil, also affect the rate of temperature rise and thus can be used to tune the rate and the amount of droplet heating in a given experimental configuration.
Fluorescence resonance energy transfer (FRET) relies on the distance-dependent transfer of energy from a donor fluorophore to an acceptor fluorophore. Due to its sensitivity to distance, FRET has been used as a tool for measuring nanometer scale distances and changes in distances, both in vitro and in vivo.46 The FRET technique is also used in biology as a nano-sized thermo-sensor to observe the heating of small areas by conjugating complementary oligonucleotides with FRET pair fluorophores.42,47,48 This technique takes advantage of the temperature dependence of the reversible hybridization of oligonucleotides. In this study, complementary DNA strands were end-labeled with the donor-acceptor pair Cy3-Cy5. When allowed to hybridize, the close proximity of the dye molecules allows for FRET to occur, and upon excitation with blue (470 nm) light, red emission from Cy5 at 670 nm is observed. As the solution is heated and the duplex melts, the dyes separate. Emission from Cy3 is then observed at 570 nm, corresponding to a decrease in efficiency of the resonant energy transfer process. The relationship between the Cy5/Cy3 emission intensity ratio and temperature allows the fluorescence spectrum to be used to measure droplet temperature.
As described in the previous section, two droplets containing Cy3- and Cy5-conjugated complementary oligonucleotides were half-covered with magnetic porous Si microparticles. This half-coverage of the droplets by the particles allows better detection of fluorescence changes with the CCD detector. When the two droplets are merged at room temperature (Fig. 4b), hybridization of complementary oligonucleotides occurs. The merged droplet is then heated by application of an alternating electromagnetic field. The electromagnetic field is switched on for 25 s (5 s elapsed before maximum power was reached), and then off for 95 s. This cycle was repeated five times. The fluorescence spectra of the droplet during the heating process (Fig. 7a) display a change in the Cy5/Cy3 fluorescence intensity ratio with time. The temperature of the droplet was independently monitored by insertion of a thermocouple probe before and after the heating intervals (Fig 7b). Since the calculated melting temperature of the FRET probe is 46.8 °C, the droplet temperature was cycled between 37 °C and 57 °C during the heating/cooling intervals. During heating, the Cy5/Cy3 intensity ratio decreases from a maximum value of ~1.5 (at ~37 °C) to a minimum value of ~0.5 (at ~57 °C). The reproducibility of the results during the five consecutive heating/cooling cycles demonstrates that the temperature of the droplet can be precisely controlled and the DNA duplex is durable enough to allow consistent hybridization and de-hybridization transitions in the presence of the magnetic porous Si microparticles. It is possible that some non-specific adsorption of the analytes to the microparticles occurs under the experimental conditions, although no evidence of this was found in the present study. The reproducibility of Cy5/Cy3 fluorescence intensity ratio over the five consecutive heating/cooling cycles indicates that non-specific adsorption of the DNA probes, if present, does not significantly impair the assay.
The temperature change measured by Cy5/Cy3 fluorescence intensity ratio correlates with the temperature measured with the external thermocouple probe (Fig. 8). Independent measurements on a particle/droplet assembly that was heated with a laboratory hotplate instead of with the RF coil produce the same correlation. With both heating methods, the Cy5/Cy3 fluorescence intensity ratio shows a similar decrease with increasing temperature.
This study demonstrates the feasibility of using encoded magnetic amphiphilic porous Si microparticles to locally heat, manipulate and identify discrete microliter-scale liquid droplets. The droplets in the present system can be heated and moved individually. As the level of heating is related to the amount of microparticles and their degree of magnetization, a group of discrete droplets can be simultaneously heated to different temperatures using a single coil. In addition, the one-dimensional photonic crystal etched into the porous Si particle provides a unique spectral code to identify a droplet. Thus the approach enables assays to be processed in any desired order in parallel fashion; for example in randomized or combinatorial arrays.
Furthermore, this droplet system overcomes a requirement of conventional conductive heating schemes – thermal contact between the heating element and reaction vessel. Thus, a chemical reaction requiring temperature control can be remotely actuated. The scheme potentially can be applied in vivo, using extracorporeal application of electromagnetic fields to activate and/or release therapeutic agents from inside a droplet.
This project has been funded in part with Federal funds from the National Cancer Institute of the National Institutes of Health (Contract No. N01-C0-37117) and the Air Force Office of Scientific Research (Grant# F49620-02-1-0288). M.J.S. and S.N.B. are members of the Moores UCSD Cancer Center and the UCSD NanoTUMOR Center under which this research was conducted and partially supported by NIH grant U54 CA 119335. J.P. thanks the Korea Science and Engineering Foundation (KOSEF) for a Graduate Study Abroad Scholarship. A.M.D. thanks the Graduate Research and Education in Adaptive Bio-Technology (GREAT) Training Program of the University of California Biotechnology Research and Education Program for a graduate fellowship. The authors thank Jason Dorvee, Sara Alvarez, and Dr. Greg Underhill for helpful discussions, and Evelyn York of the Scripps Institute of Oceanography, Analytical Instrument Facility, for assistance with the scanning electron microscopy measurements.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text: Ten Gaff Cards That Anyone Can Do, (Magic, Tutorial) by kilemc in magic-tricks Download Share Favorite I Made it! Collection IntroIntroduction: Ten Gaff Cards That Anyone Can Do, (Magic, Tutorial)10 gaff cards that you can do in home. CommentsLord_Lucas (author)Reply2017-02-24it would be great if you could do a video in englishLord_Lucas (author)Reply2017-02-24I don't know spanishMore CommentsAbout This Instructable 575views2favoritesLicense:kilemcFollow62More by kilemc:Add instructable to: Contest GroupRelated5 Ways to control a card 2.0 (Magic trick)by kilemcPuzzle (David blaine Card trick) Tutorial, performance.by kilemc5 Cortes falsos fáciles de hacer (Magia tutorial)by kilemc5 magic tricks easy to do. (Part 3)by kilemc5 magic tricks easy to do.by kilemc


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Examples of poultry in a Sentence
This wine goes well with poultry.
Recent Examples of poultry from the Web
The 365-branded poultry at Whole Foods is often rated Step 2, since GAP doesn't certify chickens at a lower level.
Restaurant operators can also throw different types of wood, such as apple, cherry, hickory or mesquite, on top of the coal to add flavors when roasting meat, poultry or vegetables in the oven, Hofmann explained.
Pig racing, farm animal petting farm, livestock and poultry displays, blacksmith demonstration, remote control car racing, wood carving display, big top circus, demolition derbies and much more.
His letter aims at protected Canadian sectors such as telecoms and financial services (and, less explicitly, dairy and poultry farming).
Their scenario begins with impoverished residents watching their pigs and poultry fall ill, suffering high fevers and bleeding from the mucous membranes in their mouths and noses.
Harold's Chicken Shack and Sports Bar is coming to Hollywood Boulevard, bringing along 67 years of poultry-frying expertise, and a deliciously brutal logo featuring a cleaver-wielding chef chasing a chicken.
Since it was signed, Canada and Mexico have become by far America's largest export markets for products like corn, pork, beef, dairy, soybeans and poultry, and overall total exports have grown from $9 billion in 1993 to $41 billion by 2014.
Under a U.S. Department of Agriculture Food Safety and Inspection Service (FSIS) program, thousands of samples of meat and poultry products are analyzed each year, looking for residues of certain drugs, pesticides and other contaminants.
These example sentences are selected automatically from various online news sources to reflect current usage of the word 'poultry.' Views expressed in the examples do not represent the opinion of Merriam-Webster or its editors. Send us feedback.
Origin and Etymology of poultry
First Known Use: 14th centurySee Words from the same year
POULTRY Defined for English Language Learners
POULTRY Defined for Kids
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Spanish Central: Translation of poultry Nglish: Translation of poultry for Spanish speakers Britannica English: Translation of poultry for Arabic speakers Britannica.com: Encyclopedia article about poultry
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The 24 Coolest Military Birds In Flight Right Now
Wikimedia CommonsEvery now and then it's necessary to put a quick list of cool things together.
Today, it's military birds from around the globe.
And they could be cool for whatever reason: stealth, long range, armaments, maneuverability or ... Rule #1: Looking Cool.
Whether it's a sleek design, or pure unadulterated deadliness, these are the best and baddest in the air.
Click here to see who made the list »
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Florida Polytechnic University Chief Operating Officer Ava Parker has hired three key employees to help create the 12th state university.
LAKELAND | Florida Polytechnic University Chief Operating Officer Ava Parker has hired three key employees to help create the 12th state university.
Coming on board soon will be two communications specialists and a vice president of administration.
Parker began her job as interim head of the school in late December. Until now, the multi-faceted job of starting the school has been addressed mostly by a volunteer board of trustees and contracted help, including University of Florida staff.
Parker said it will be great to have people on board to work on communication with board members and the media, keeping up the university's website, reaching out to people in the community and letting people know what the university will offer.
The new staff members are:
Joanne Chive, vice president of communication and marketing. She will be paid $90,000 annually. Chive has been director of communications for the University of Central Florida College of Business since 1999. She has a degree in communications from Niagara University, a master's and a doctor's of education from UCF.
Regina "Gina" Deiulio will be vice president of admissions; assistant corporate secretary. She will earn $140,000 a year. She has been associate general counsel for the Office of the General Counsel at Florida International University since 2005. Because the polytechnic has its own general counsel. Deiulio will use her legal expertise in her role but not be the school's official legal representative, Parker said.
Maggie Mariucci will be director of external affairs and interim director of the foundation. Her compensation will be $92,500 a year. Mariucci, who has a journalism and communications degree from the University of Florida, was the director of development, government and community affairs at the University of South Florida Polytechnic. She is the sister of Florida House Budget Chairman Rep. Seth McKeel, whose role could impact the polytechnic's future in the upcoming session.


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June 7 Service: 7:15pm at Grace Baptist Church
Please join us for a special service on June 7 at 7:15pm at Grace Baptist Church, located at 484 East San Fernando Street (10th and San Fernando). We are considering moving to Grace Baptist, and this service will help us get a feel for what worship there would be like.
We will also have a tour of the Grace Baptist facilities starting at 6:15pm. Please join us to explore the space together.
Grace Baptist has a parking lot, which you can enter off of 10th Street. On evenings and weekends, you can park in any spaces, whether they are marked as "Church," "Visitor" or "Community Center." Please click the following link for a map showing Grace Baptist.
If you'd like to learn more about Grace Baptist, you can click the following link to see a comparison of our current location and Grace Baptist. The categories in this comparison are based on a congregational survey we did in 2008. You can also click the following link to download a copy of the 2008 survey results.


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Created to provide your Dyna with the attitude and rear suspension travel that a proper corner eating machine deserves. The main spring coil absorbs big hits and prevents bottoming, while the secondary coil smooths out minor road imperfections.
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A huge congratulations to ECS teacher Laura Selby, who was just named Mercer Island Preschool Association's Exceptional Educator 2016!
Each year, MIPA honors a teacher, assistant, or administrator with the Exceptional Educator Award. The award is presented to an individual who has made extraordinary contributions to our preschool community. Teacher Laura, as so many children and families know her, has taught preschool for more than 20 years and has been at ECS since 1995 – and we're so lucky to have her.
MIPA received passionate and remarkable nomination letters from both parents and colleagues. The letters described the positive impact Teacher Laura has had on the families, children, and workplace of the J's Early Childhood School. Laura is a respectful, knowledgeable, innovative, and caring teacher.
One parent wrote, "Laura's passion for early childhood education and the belief that children are capable learners with the ability to take on responsibility and tackle hard problems has allowed the children in the class to mature and feel confident far beyond what I, even as an educator myself, would expect from two- to three-year-olds." Another person remarked, "She's so good, I worry we'll never have a teacher we like as much and will always be a tad bit disappointed."
On Friday, May 13, Laura was surprised by ECS children, parents, colleagues, and MIPA Board members that had gathered to celebrate her big achievement. The award was presented by MIPA Vice Presidents Jessica Apodaca and Amanda Colburn (pictured above with Laura, center).
Learn more about our amazing Early Childhood School, where teachers like Laura Selby are changing kids' lives every day!


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Category: Voluntaryist Sutra
Is it REALLY Selfish to be Selfish, even if You're a Christian?
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Hillary Clinton Calls for Positive Gangs- Google Voluntaryism
We do a little video mashup on Hillary Clinton's recent statement calling for "positive gangs." In her mind, those positive gangs might be the police. We beg to differ. The ONLY positive gangs are Voluntaryist ones.
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The Dose | Your Top 5 Stories of Statism Run Amuck- March 29th, 2016
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Fake State Senator? You Da Man!
The Dose | Your Top 5 Stories of Statism Run Amuck- March 29th, 2016
Fake News Anti-Assad Journalist Gets Owned
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From Freeqo- Thanks to pressure from outside groups, like the pro-zombie Gaystapo, aka the Gay...
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The Soft Control Human Hack of the Private Market Rise of "Chip Culture"
Have you ever heard of the 'Standing in Waiting Room" experiment? If you haven't, here's the video of what I'm talking about, though I'll also give you a brief summary of the video: In this video, the test subject does not know...
Hey Facebook, You Better Answer to this US Senator
This reaction from a Republican Senator to Facebook shows just how oppressive both the left AND the right really are.
Google Voluntaryism
Voluntaryist Sutra- We take the challenge to Google Voluntarism. You've seen all the memes...
The Church of State Moves To Take Over Gender Definitions…for the kids…
My brief thoughts on what's really going on with Barack Obama's latest 'decree' ordering public schools to allow transgenders to choose which bathrooms and lockers they use: