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September 21, 2017
Swiss Imams Charged For Jihadtube Activities
I've seen these videos, while they appear at first to be informational, they are really to provide a supporting platform for terrorists but without the al-Furqan watermark.
Three senior leaders of one of Switzerland's most visible Islamic organizations were indicted today after a nearly two year investigation into videos that one of the leaders made in Syria, including interviews with senior al-Qaeda leaders.
The charges were announced today by the Office of the Attorney General, which will be heard by the Federal Criminal Court...The OAG alleges that the accused offered the leading al-Qaeda member in question a prominent multilingual multimedia platform from which to advantageously portray and promote both himself and the ideology of al-Qaeda, the terrorist organisation he represents.
The OAG claims to have proof that this increased the appeal of al-Qaeda to existing and potential members around the world, thus promoting the organisations criminal activities.
Dear Rasmea Odeh
Don't let the door hit you where Allah split you.
Today was the day. Convicted terrorist murderer and immigration fraudster Rasmea Odehs last day on U.S. soil.
Shes been stripped of her citizenship and put on a plane out of OHare for Jordan, where shell join convicted terrorist murderer Ahlam Tamimi, the mastermind of the Sbarro Pizzeria Massacre.
September 20, 2017
Feed Good Video of The Day: Kilroy Drones ISIS Execution
Look at all the little Daeshbags flee!
September 19, 2017
Meanwhile In Mexico City (104 Killed)
Terrible earthquake in Mexico City.
Images from #earthquake in #MexicoCity #mexicoearthquake pic.twitter.com/x9EqKuX4og— Claudia Nardi (@ClaudiaNardi84) September 19, 2017
Xochimilco, Mexico City during the earthquake pic.twitter.com/2urUXK85ES— Danny Konstantinovic (@Dannykons) September 19, 2017
Devastating images from Mexico City. pic.twitter.com/RpF7sUq31s— Jorge Guajardo (@jorge_guajardo) September 19, 2017
Update: Its horrible... And on the Anniversary of THE big Mexican quake.
We pray for Mexico City. Despite, you know, things. We love you.
Here is some late coverage at Dallas Morning News' Website:
MEXICO CITY At least 104 people are believed dead after a magnitude 7.1 earthquake rocked central Mexico on Tuesday, collapsing buildings and scattering rubble on streets less than two weeks after another quake left 90 dead in the country's south.
Mexican media broadcast images of multiple downed buildings in densely populated parts of Mexico City and nearby Cuernavaca to the south. To the east, in Cholula, the towers of some of the city's famous churches had fallen, Interior Secretary Diodoro Carrasco of Puebla State said.
And Now a Message of Religious Extortion From the Religion of Peace
CNN Documentary on Sharia4Belgium Losers
Especially Fouad, he needs a one way ticket to hell.
Hey Fouad, remember back when I obliterated all your websites? Good times. Good times.


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Have Medical Questions? Ask a Doctor Online!
Hi, I'm Dr Chaithanya, an experienced General Physician attending you today.
The condition of excessive sweating is called Hyperhydrosis. It can be primary without any causes, called Idiopathic or primary hyperhidrosis or it can be secondary due to hormonal conditions like hyperthyroid or few vitamin deficiencies.
There can be more sweating at places that are termed as selective hyperhidrosis or primary focal hyperhidrosis.
The yellow color is due to the sweat interacting with bacteria that is present on the skin, and after interaction it can leave an odor and a yellow stain.
Possible treatment options are,
1. Wearing loose cotton clothes and maintaining cleanliness
2. Using mild antiperspirants
3. Periodic Botulinum toxin injection
4. Destruction od exocrine sweat glands at the places of excessive sweat using microwave energy/laser
You need to discuss the options with the dermatologist so that he can suggest you the right treatment based on your body conditions.
Hope the information helps!
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subset: commoncrawl


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La Cucina (international title: With Wine and Lovers)
written by Allison W. Gryphon
directed by Allison Hebble & Zed B. Starkovich
starring: Christina Hendricks, Joaquim de Almeida, Leisha Hailey and Rachel Hunter
BEST PICTURE Backlot Film Festival BEST SCREENPLAY Bragacine International Festival of Independent Cinema de Braga BEST PICTURE Beloit International Film Festival BEST ACTORBragacine International Festival of Independent Cinema de Braga OFFICIAL SELECTION La Femme Film Festival OFFICIAL SELECTION Portocine International Film Festival OFFICIAL SELECTION Hollywood Film Festival PRODUCERS AWARD Bragacine International Festival of Independent Cinema de Braga
Screenwriter A.W. Gryphon with Executive Producer Starlotte Dawn Smith and Director/Editor Zed B. Starkovich.
Entertainment Weekly – Move over Julia Child, Christina Hendricks is in the kitchen!
La Cucina is described as "a romantic drama about life, love and the joy of cooking." But what you really need to know is that it marks the film debut of Mad Men's beloved Joanie, Christina Hendricks.
Glamour – Want To Know What Lip Color Christina Hendricks Is Wearing Here? Or What's Making Her Skin Look Extra Glowy? The Makeup Artist Spills All!
In honor of the movie La Cucina starring Christina Hendricks (and best-feature-winner at the Los Angeles Backlot Film Festival) hitting DVD this week, I got the makeup artist, Noreen Wilkie to spill all the details behind Christina's gorgeous look in the film.
AfterEllen – Leisha Hailey and Christina Hendricks gather in "La Cucina"
What would it take for you to see a movie most of you have never heard about before? What if I told you it involved food? My Top Chef people are already there. How about if I mentioned it starred Leisha Hailey? Now I've hooked a bunch of you. And what if I added it also starred Christina Hendricks. More of you, good. And, finally, what if I were to casually say supermodel Rachel Hunter played a lesbian. And, we have a winner.
LA Splash – La Cucina: An Alison Hebble/Zed B. Starkovich Film
La Cucina, starring Rachel Hunter, Christina Hendricks and Joaquim de Almeida and directed by Alison Hebble, deals with the relationship complexities of three different couples and how they cope with the reality of impending events.
About.com – La Cucina – Lesbian Movie Starring Leisha Hailey
A Slice of Life. Three women in their 30s struggle with love and life, all while cooking and eating in various kitchens in an apartment complex that resembles a Tuscan villa.Somewhere in Hollywood, these women are cooking (or not) and discussing their (and others') relationships. Lily (Christina Hendricks), a vivacious redhead, is an aspiring writer; Shelly (Leisha Hailey) is a pregnant wife with a dull husband and raging hormones; Jude (Rachel Hunter), is a fabulous cook and very sensible lesbian.


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Panduit Opti-Core Push-Pull - Patch cable - LC multi-mode (M) to LC multi-mode (M) - 10 ft - fiber optic - 50 / 125 micron - OM3 - booted, riser - aquaItem#: 13739972 | Model#: FX2ERQ1Q1ONM003
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Panduit Opti-Core Push-Pull - patch cable - 10 ft Product Details
Network Cable Type: Patch cable - OM3
Technology: Fiber optic
Type of Fiber Optics: Multimode
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If you have a business where there are a significant number of valuable items that you would prefer not having stolen, Perfect Connections Security Systems said it's a good idea to have some type of security system installed. If you don't, there is always the possibility that vandals can break in, and take everything that you own. There are many different types that you can install, and modern technology provides us with many benefits. Let's take a look at some of the latest technology implemented into alarms and security systems to show you why this is so essential for your company.
Modern Alarm Systems
Alarm systems used to be very basic. There would actually be some type of alarm that would sound off, alerting people that an intruder had broken in. This later advanced into alarm systems that would notify the police, and then third party providers stepped in. They are actually able to come out to your location, provide you with all of the high-tech equipment, and you simply pay them a monthly fee to have access to not only their equipment but the services that they provide. Alarms are constantly monitored, and they will immediately notify the authorities once it is triggered, giving you the fastest response possible from these state-of-the-art fully monitored alarm systems.
Remote Access Capabilities
The capabilities that are now included with many alarm systems, whether you install this yourself or have a company do it for you, include the use of remote access, direct to your smart phone, allowing you to see what is going on. This can be beneficial as the alarm may actually be triggered by someone that you know, allowing you to make the decision as to whether or not to call the police. It also gives you a secondary way of recording what is going on in case you need to use this video in the prosecution of those that break-in.
Technology really has made alarm systems very beneficial. Both residential and business alarms have come a long way. If you would like to gain access to state-of-the-art security systems that use all of this modern technology, even including infrared cameras that can capture images and video at night, you should consider having one of these installed in the near future. If you decide to do this yourself, or have a company provide this for you, either one is going to give you the protection that you need. It will keep all of your belongings safe at your place of business using these modern alarm systems that currently protect millions of people and businesses.


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Difference between Cigarette and Electronic Cigarette
Hon Lik invented the electronic cigarette because of his father – who died due to lung cancer. Lung cancer is the most common type of disease that kills millions of people every year all over the world. Is it really an effective alternative to the traditional cigarette?
The difference of cigarette and e-cigarette
Aside from the physical difference, these two greatly varies on how the smoke is produced. In traditional cigarette, the lighter is used to burn the solid nicotine and other associated dangerous chemical then turn these into smoke. The resulting smoke can harm the smoker's body due to the cancer-causing elements and other harmful chemical compounds that it contains. On the other hand, electronic cigarette only uses a liquid solution that contains nicotine, flavorings, and other chemicals. When the user puffs the electronic cigarette, the heating device starts to work by vaporizing the liquid solution. As a result, it will produce an aerosol or vapor. This smoke is only a water vapor and won't cause any health problems to the users.
The traditional cigarette contains up to 4000 harmful chemical products and 43 of these are cancer-causing elements. While the electronic cigarette doesn't use tobacco and has only two carcinogens: diethylglycol and TSNA. In terms of usage, cigarette needs heat so you can use it, while you only need a rechargeable battery for the electronic cigarette.
Another essential difference of these two is the pollution that they cause. The cigarette is known to cause lung cancer to secondhand smokers. The secondhand smokers are people who inhale smoke given off by burning tobacco product or exhaled by the direct smokers. Moreover, the environment is also affected due to the carcinogens and toxic carbon dioxide gas found in cigarettes.
Due to the positive aspects of electronic cigarettes, many people and organizations are encouraging people to use this. They impart essential information regarding its usage through meetings and events. They even give coupon codes to people who participated containing discounts and freebies.
Can electronic cigarette help smokers quit?
It is still unclear whether electronic cigarette can help smokers quit or not. There are still not enough data and research studies to evaluate its usage. Some people believe that it can lower nicotine cravings and encourage smokers to discontinue smoking cigarettes. However, others think that it's possible for the product to affect nicotine addiction.


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Achieving mitigation and adaptation to climate change through sustainable agroforestry practices in Africa
Agroforestry is one of the most conspicuous land use systems across landscapes and agroecological zones in Africa. With food shortages and increased threats of climate change, interest in agroforestry is gathering for its potential to address various on-farm adaptation needs, and fulfil many roles in AFOLU-related mitigation pathways. Agroforestry provides assets and income from carbon, wood energy, improved soil fertility and enhancement of local climate conditions; it provides ecosystem services and reduces human impacts on natural forests. Most of these benefits have direct benefits for local adaptation while contributing to global efforts to control atmospheric greenhouse gas concentrations. This paper presents recent findings on how agroforestry as a sustainable practice helps to achieve both mitigation and adaptation objectives while remaining relevant to the livelihoods of the poor smallholder farmers in Africa.
The Cavaliers handled their
The Cavaliers handled their business last night as they easily took care of the Celtics. LeBron and Love has great performances while Kyrie had a quite 11. Kyrie may not have been on his Kobe Shoes "A" game, but his kick game, that's another story.Above you will get a KD 9 Shoes first look at what is known as the Nike Kyrie 3 School Bus. This unreleased colorway of the Kyrie 3 rocks the same these as the School Bus colorway we say on the Kyrie 2. It comes with a Yellow upper with details such as the "STOP" sign on the Kobe 5 tongue, and "School Bus" branded on the heel. Black and White detailing found throughout give the shoe some sharp contrast. No release date yet but if/when they do, Adidas Harden Vol 1 expect them to be a GS-exclusive.The Nike Air Shake NDestrukt is releasing in a triple white colorway that will be a perfect pair to rock this summer. Kobe 11 Dennis Rodman's classic silhouette from the '90s is colored in all-white and features a mix of leather and mesh with perforated detailing on the toecap for its upper. Lebron 14 Shoes The shoe's signature details are its asymmetrical lacing system, oversized swooshes on the tongue, and ankle strap, while sitting on top of a matching rubber sole.Retailing for a Lebron 13 price tag of $140, look for the Nike Air Shake NDestrukt Triple White at select Nike stores and online soon. Always keep it locked to KicksOnFire for more Kyrie 1 updates and the latest in sneaker news. While its last release to the public consisted of a midnight navy variation, the Nike Kobe A.D. is rendered in Kobe AD Shoes a vibrant color scheme of black and yellow next.With this pair similar to the recently featured Oregon Ducks PE, although in different colors, this silhouette is suggested to Kyrie 3 Shoes be Ducks-inspired as well, with the notable "O" logo prominent on its heel tab. In addition, a feather graphic is seen across its midsole unit.Retailing for $160, this Kevin Durant Shoes latest pair is suggested to make its debut at select Nike retailers starting March 10th.Stay tuned to KicksOnFire for further updates. This year is the 20th anniversary Lebron 14 of the Nike Air More Uptempo and it will be releasing in an all-black colorway soon, as one of its newest iterations. Sporting the large "AIR" branding across Lebron Soldier 9 its sides, that detail features 3M reflective accents while the sneaker's entire upper is made of smooth nubuck. Striped reflective laces and a matching rubber sole unit that Harden Vol 1 Shoes include its signature visible air bubble throughout completes the overall design.Look for the Nike Air More Uptempo Triple Black at select Nike stores and online on April 15 Kd 5 for a retail price tag of $160.Materials: Straight out of the box, the ballistic mesh-like upper feels extremely stiff, but after a standard break-in period the materials KD 9 Elite end up being slightly above average. Nike calls the mesh material they used here "breathable" but the only part of the Kobe A.D. that allows heat to escape Lebron 11 and air to flow is the mesh tongue. The rest off the upper might as well be made of plastic from a breathability standpoint. The good news is Kobe 10 that the mesh upper gets more comfortable every time you slip your foot in them and by the time it's fully broken in, the upper goes unnoticed during Kyrie Shoes use — a good thing, as it won't distract you during playing time. There is a suede overlay in the heel area but this just gives the A.D. James Harden Adidas Shoes a boost in aesthetic appeal and has no effect whatsoever in its performance on-court.Did LeBron James flop on Draymond Green's flagrant foul?Last night the Golden State Warriors kyrie irving shoes blew out the Cleveland Cavaliers in their final regular season matchup. The game had its moments but the Draymond/LeBron incident was one of the more exciting moments in KD 9 the game and we break it all down here on the latest episode of NBA Talk.Let us know what you think about the play in the comments section LeBron Soldier 10 below and don't forget to give this video a like!Sadly, she passed away in 2000, long before he was "KD." When Nike learned of this influential person KD Shoes in Durant's life, the brand agreed to make a colorway of KD's signature shoes every year in her honor. We have seen angel wings, pink roses, and many LeBron Ambassador 9 other details, but this might be the best of the series.The KD9 'Aunt Pearl' features a black Flyknit forefoot with pink threads running through it. The heel portion Kobe 1 is black neoprene with various inlays of the initials "AP." That heel KD logo, sewn in, really gives off a lux feel, while down below, the Max Zoom Lebron 12 bubbles get the pink touch. The Kay Yow tag hits the pull tab with the pink ribbon sewn in, signifying that a portion of the proceeds will go Kyrie 3 to the Kay Yow Cancer foundation, an absolutely worthy cause.The KD9 'Aunt Pearl' hits SNKRS tomorrow but will hit Nike.com and retail shelves next Saturday, January 28, for Kyrie 2 $150 — the same price as the regular version.The latest Black/White Curry 3 colorway, and eight others, are currently on sale for $112 each.To pick up the Lebron 10 Curry 3 at a slight discount, you can head to Finish Line by clicking here. The Trifecta Black, Raw Sugar, TTC, Red Hot Santa, Dub Nation, and Artillery Kobe A.D. NXT Green are just some of the builds available.For performance reviews on the Curry 3: Quick Kicks, Duke4005.Shout to @FlashSole for keeping people up to date on discounted Under Kobe AD Armour product.Under Armour has unveiled the Curry 3Zer0 that will be released via a scavenger hunt with Foot Locker throughout three locations in New York City.Curry has Harden Shoes announced the giveaway via Twitter on Friday. He will be giving clues on where to find the shoes on his Twitter at 10AM EST tomorrow, March 4th. Each Nike Kyrie 3 pair will be autographed by Steph himself.According to Under Armour, the White/Gold Curry 3Zer0 will not release in retailers. But you can expect other colorways to debut for Lebron Shoes the playoffs beginning May 1st. The retail price tag is set at $120 USD.LeBron James debuted the LeBron 14 in a win over the Golden State Warriors Lebron James Shoes on Christmas Day, and even though the Warriors got their revenge on Monday night in a blowout victory, LeBron and the Cavs didn't seem too worried about it. Kyrie 3 BHM Perhaps it's knowing that there's still half of the regular season to be played that made them shrug off the loss so easily – and that only means Harden Vol 1 we'll be treated to more colorways of the Nike LeBron 14 on-court. Today we get a look at official imagery of the first retail release of LeBron's latest Kd 6 signature sneaker with the introduction of the Nike LeBron 14 Black Ice due out on January 28th, 2017 for $175 USD.The Nike LeBron 14 Black Ice is as Kobe A.D. simple a debut colorway as they come thanks to a seamless slip-on black base featuring the latest in Nike's engineered laser perforation. A midfoot strap returns featuring a James Harden Shoes unique texture complete with Swoosh branding while the outsole makes use of maximum volume Zoom Air units. What results is a LeBron signature that sheds any extra weight, Kobe Bryant Shoes skipping out on the usual Posite technology that's been such a strong asset in past LeBron releases. What do you think of the model?Stay tuned for an official Lebron 14 Low unveiling of the LeBron 14 Black Ice in the near future, grab your pair on January 28th, 2017 for $175 USD, and stay tuned for more of the Kd 8 latest LeBron updates right here on Sneaker News.
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The Life of John Locke, with Extracts from His Correspondence, Journals, and Common-Place Books
New Edition. With considerable additions.
King, [Peter, seventh baron King, of Ockham].
London: Henry Colburn, 1830. Second edition. Includes nine letters from van Limborch to Locke and the passage omitted from Limborchs letter in Some Familiar Letters narrating his discussion with the young woman who preferred Judaism to Christianity, and one letter from Locke to Engraved frontisportrait, and engraved facsimile of Locke handwriting. A very good set. Half calf over marbled boards, rebacked in later calf with old burgundy morocco labels preserved. Two volumes, octavo. viii, 447; [2], 375, [1, (Item ID: 11654)
King (1776-1833), a descendant of Lockes cousin, Anne Locke, wrote the second major biography of John Locke, after LeClercs life of Locke, which appeared in the Bibliothèque Choisie in 1705. It added a great deal to what had previously been known about Locke, as King was able to draw upon extensive correspondence, and Lockes personal papers.
Yolton 329.


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Henrotin, Yves ; ; Sanchez, Christelle
Patent (2011)
The present invention relates to a method of producing a hydrogel matrix comprising cartilage-forming cells wherein alginate, chitosan and cartilage- forming cells are mixed and subsequently polymerised ... [more ▼]
The present invention relates to a method of producing a hydrogel matrix comprising cartilage-forming cells wherein alginate, chitosan and cartilage- forming cells are mixed and subsequently polymerised into beads. [less ▲]Detailed reference viewed: 54 (5 ULg)
Cell cycle activation of hematopoietic progenitor cells increases very late antigen-5-mediated adhesion to fibronectin.
Giet, Olivier ; ; Beguin, Yves et al
in Experimental hematology (2001), 29(4), 515-24
Recent studies suggested that trafficking of hematopoietic progenitor cells is related to cell cycle status. We studied whether adhesion of progenitor cells to extracellular matrix proteins was modulated ... [more ▼]
Recent studies suggested that trafficking of hematopoietic progenitor cells is related to cell cycle status. We studied whether adhesion of progenitor cells to extracellular matrix proteins was modulated by cell cycle transit.Mobilized peripheral blood CD34+ cells were stimulated ex vivo for 48 hours with stem cell factor, flt-3 ligand, and thrombopoietin and fractionated by adhesion to fibronectin or vascular cell adhesion molecule-1 (VCAM-1). Adherent and nonadherent cells were assayed for cell cycle status, long-term culture-initiating cell frequency, and integrin function. Binding to fibronectin, but not to VCAM-1, displayed a cell cycle selectivity as the adherent fraction to fibronectin was enriched in cycling CD34+ cells and in cycling long-term culture-initiating cells compared to the nonadherent fraction. Combined cell cycle and phenotypic analysis showed that the expression of VLA-5 was upregulated during S/G2+M but that of VLA-4 remained constant. The selective binding of cycling CD34+ cells to fibronectin was reverted by anti-VLA-5 but not by anti-VLA-4 blocking antibodies. Also, cycling CD34+ cells preferentially adhered to the VLA-5 binding domain but not to the VLA-4 binding domain of fibronectin. Adhesion of cycling CD34+ cells to fibronectin was a reversible process modulated by cell cycle progression, because adherent cells could exit the cell cycle and return to a nonadhesive state within an additional 48-hour culture period.The results indicate that the enhanced binding capacity of cycling progenitor cells to fibronectin is mediated by VLA-5. [less ▲]Detailed reference viewed: 46 (4 ULg)
Cell cycle duration at the time of maternal zygotic transition for in vitro produced bovine embryos: effect of oxygen tension and transcription inhibition.
Lequarré, Anne-Sophie ; ; et al
in Biology of Reproduction (2003), 69(5), 1707-13
Early embryonic cleavages are mostly regulated by maternal components then control of development progressively depends on newly synthesized zygotic products. The timing of the first cleavages is a way to ... [more ▼]
Early embryonic cleavages are mostly regulated by maternal components then control of development progressively depends on newly synthesized zygotic products. The timing of the first cleavages is a way to assess embryo quality. The goal of this study was to evaluate the duration of the fourth cell cycle, at the time of maternal-to-zygotic transition (MZT) in in vitro-produced bovine embryos by means of cinematographic analysis. We found that 75% of the embryos displayed a long fourth cycle (43.5 +/- 5.4 h) whereas the remaining embryos had a very short fourth cell cycle (8.9 +/- 2.9 h). Both groups did not differ in cleavage rhythm up to the eight-cell stage and timing of cavitation and blastocyst expansion was identical. However, embryos with a short fourth cell cycle had a better blastocyst rate than embryos with a long cycle (59% versus 38%, P < 0.01). Total cell number, inner cell mass (ICM):total cell ratio, and hatching rate were identical for blastocysts produced from embryos with either a long or a short fourth cell cycle. In a second experiment, we showed that increasing the oxygen tension, from 5% to 20%, decreased the percentage of embryos with a short fourth cell cycle, from 25% to 11% (P < 0.01), indicating that suboptimal culture conditions can influence the length of this cycle. Finally, we investigated whether fourth cell cycle duration could be influenced by transcription inhibition. With alpha-amanitin added at 18 h postinsemination (HPI), cleavage was reduced (66% versus 79%) and, at 70 HPI, the 9- to 16-cell rate increased (50% versus 25%) concomitantly with a 5- to 8-cell rate decrease (16% versus 47%). A similar pattern was observed when the drug was added at 6 HPI or 42 HPI but not at 0 HPI. Cinematographic analysis revealed that alpha-amanitin increased the first cell cycle duration whereas the second and third cell cycles were not affected. With the drug, one third of the embryos could develop up to the 9- to 16-cell stage and they all had a short fourth cell cycle (11.2 +/- 3.7 h) with a good synchrony of cleavage between blastomeres. These results suggest that duration of the fourth cell cycle of bovine embryo, during the MZT, is under a zygotic transcriptional control that can be affected by oxidative conditions. [less ▲]Detailed reference viewed: 40 (2 ULg)
Cell cycle S phase markers are expressed in cerebral neuron nuclei of cats infected by the Feline Panleukopenia Virus
; Garigliany, Mutien-Marie ; et al
in Cell Cycle (Georgetown, Tex.) (2016), 15(24), 3482-3489
The cell cycle-associated neuronal death hypothesis, which has been proposed as a common mechanism for most neurodegenerative diseases, is notably supported by evidencing cell cycle effectors in neurons ... [more ▼]
The cell cycle-associated neuronal death hypothesis, which has been proposed as a common mechanism for most neurodegenerative diseases, is notably supported by evidencing cell cycle effectors in neurons. However, in naturally occurring nervous system diseases, these markers are not expressed in neuron nuclei but in cytoplasmic compartments. In other respects, the Feline Panleukopenia Virus (FPV) is able to complete its cycle in mature brain neurons in the feline species. As a parvovirus, the FPV is strictly dependent on its host cell reaching the cell cycle S phase to start its multiplication. In this retrospective study on the whole brain of 12 cats with naturally-occurring, FPV-associated cerebellar atrophy, VP2 capsid protein expression was detected by immunostaining not only in some brain neuronal nuclei but also in neuronal cytoplasm in 2 cats, suggesting that viral mRNA translation was still occurring. In these cats, double immunostainings demonstrated the expression of cell cycle S phase markers cyclin A, cdk2 and PCNA in neuronal nuclei. Parvoviruses are able to maintain their host cells in S phase by triggering the DNA damage response. S139 phospho H2A1, a key player in the cell cycle arrest, was detected in some neuronal nuclei, supporting that infected neurons were also blocked into the S phase. PCR studies did not support a co-infection with an adeno or herpes virus. ERK1/2 nuclear accumulation was observed in some neurons suggesting that the ERK signaling pathway might be involved as a mechanism driving these neurons far into the cell cycle. [less ▲]Detailed reference viewed: 14 (0 ULg)
Cell cycle-related changes in repopulating capacity of human mobilized peripheral blood CD34(+) cells in non-obese diabetic/severe combined immune-deficient mice.
GOTHOT, André ; ; et al
in Blood (1998), 92(8), 2641-9
Most primitive hematopoietic progenitor cells reside in vivo within the G0/G1 phase of the cell cycle. By simultaneous DNA/RNA staining it is possible to distinguish G0 and G1 states and to isolate cells ... [more ▼]
Most primitive hematopoietic progenitor cells reside in vivo within the G0/G1 phase of the cell cycle. By simultaneous DNA/RNA staining it is possible to distinguish G0 and G1 states and to isolate cells in defined phases of the cell cycle. We report here the use of cell cycle fractionation to separate human mobilized peripheral blood (MPB) CD34(+) cells capable of repopulating the bone marrow (BM) of non-obese diabetic/severe combined immune-deficient (NOD/SCID) mice. In freshly isolated MPB, repopulating cells were predominant within the G0 phase, because transplantation of CD34(+) cells residing in G0 (G0CD34(+)) resulted on average in a 16.6- +/- 3.2-fold higher BM chimerism than infusion of equal numbers of CD34(+) cells isolated in G1. We then investigated the effect of ex vivo cell cycle progression, in the absence of cell division, on engraftment capacity. Freshly isolated G0CD34(+) cells were activated by interleukin-3 (IL-3), stem cell factor (SCF), and flt3-ligand (FL) for a 36-hour incubation period during which a fraction of cells progressed from G0 into G1 but did not complete a cell cycle. The repopulating capacity of stimulated cells was markedly diminished compared with that of unmanipulated G0CD34(+) cells. Cells that remained in G0 during the 36-hour incubation period and those that traversed into G1 were sorted and assayed separately in NOD/SCID recipients. The repopulating ability of cells remaining in G0 was insignificantly reduced compared with that of unstimulated G0CD34(+) cells. On the contrary, CD34(+) cells traversing from G0 into G1 were largely depleted of repopulating capacity. Similar results were obtained when G0CD34(+) cells were activated by the combination of thrombopoietin-SCF-FL. These studies provide direct evidence of the quiescent nature of cells capable of repopulating the BM of NOD/SCID mice. Furthermore, these data also demonstrate that G0-G1 progression in vitro is associated with a decrease in engraftment capacity. [less ▲]Detailed reference viewed: 17 (0 ULg)
Cell death and growth arrest in response to photodynamic therapy with membrane-bound photosensitizers
Piette, Jacques ; Volanti, Cédric ; et al
in Biochemical Pharmacology (2003), 66(8), 1651-1659
Photodynamic therapy (PDT) is a treatment for cancer and for certain benign conditions that is based on the use of a photosensitizer and light to produce reactive oxygen species in cells. Many of the ... [more ▼]
Photodynamic therapy (PDT) is a treatment for cancer and for certain benign conditions that is based on the use of a photosensitizer and light to produce reactive oxygen species in cells. Many of the photosensitizers currently used in PDT localize in different cell compartments such as mitochondria, lysosomes, endoplasmic reticulum and generate cell death by triggering necrosis and/or apoptosis. Efficient cell death is observed when light, oxygen and the photosensitizer are not limiting ("high dose PDT"). When one of these components is limiting ("low dose PDT"), most of the cells do not immediately undergo apoptosis or necrosis but are growth arrested with several transduction pathways activated. This commentary will review the mechanism of apoptosis and growth arrest mediated by two important PDT agents. i.e. pyropheophorbide and hypericin. (C) 2003 Elsevier Inc. All rights reserved. [less ▲]Detailed reference viewed: 50 (1 ULg)
Cell death signaling in cancer cells treated by Photodynamic Therapy
Fettweis, Grégory
Doctoral thesis (2016)
Glioblastoma multiformes (GBMs) are an extremely aggressive and infiltrating type of brain cancer. Despite heavy therapies and extensive fundamental and applied research, the median survival of patients ... [more ▼]
Glioblastoma multiformes (GBMs) are an extremely aggressive and infiltrating type of brain cancer. Despite heavy therapies and extensive fundamental and applied research, the median survival of patients remains about 15 months after diagnosis for over a decade. Therefore, there is an emergency to find new approaches and therapeutic targets for treating this cancer. Photodynamic therapy (PDT) recently demonstrated a high potential in the treatment of GBM. Moreover, GBMs induced cell death by PDT is dependent of an atypical RIP3-dependent programmed necrosis. In parallel, GBMs activate a pro-survival autophagic pathway in order to recycle PDT-damaged structures and organelles. In our PhD thesis, we investigated the regulation of this autophagic process and found that TSC2 protein had an important role in autophagy activation by PDT. Indeed, PDT treatment quickly activates the kinase MK2, which phosphorylates TSC2 on serine 1254. We then showed that phosphorylation of this serine was crucial for autophagy activation, which makes TSC2 a crucial pro-survival factor in GBMs treated with PDT. Finally, we demonstrated that protein 14-3-3 ζ (YWHAZ) interacts with TSC2 and protects TSC2 serine 1254 phosphorylation from phosphatase actions after PDT. In the same time we conducted a proteomic analysis on RIP3 immunoprecipitate. The major implication of this analysis is the demonstration that RIP3 interacts with TSC2 and YWHAZ. Finally, we showed that RIP3 interacts better with the non-phosphorylated form of TSC2 than with the phosphorylated form, suggesting a RIP3 interference in the TSC2-dependent autophagy activation process. These data were submitted for publication in "Scientific Reports". In the second part of this thesis, we also investigated the influence of RIP3 expression on osteosarcoma (U2OS) cell death. U2OS expressing or not RIP3 were treated with PDT and cell death mechanisms have been investigated. We first demonstrated that in both cell lines, apoptosis was the major cell death mechanism. Secondly, we noticed an over-activation of various caspases in cells expressing RIP3 despite a stronger resistance to PDT. This could be explained by a lower activation of autophagy in cells not expressing RIP3. Thirdly, we showed that in RIP3 expressing cells, residual necrosis was RIP1-dependent. We therefore suggest that RIP3 is able to influence the cell death process. These data were published in "Laser in surgery and medicine." [less ▲]Detailed reference viewed: 40 (9 ULg)
Cell dynamics and immune response to BLV infection: a unifying model
Florins, Arnaud-Francois ; Gillet, Nicolas ; et al
in Frontiers in Bioscience : A Journal and Virtual Library (2007), 12
Bovine Leukemia virus (BLV) is the natural etiological agent of a lymphoproliferative disease in cattle. BLV can also be transmitted experimentally to a related ruminant species, sheep, in which the ... [more ▼]
Bovine Leukemia virus (BLV) is the natural etiological agent of a lymphoproliferative disease in cattle. BLV can also be transmitted experimentally to a related ruminant species, sheep, in which the pathogenesis is more acute. Although both susceptible species develop a strong anti-viral immune response, the virus persists indefinitely throughout life, apparently at a transcriptionally silent stage, at least in a proportion of infected cells. Soon after infection, these humoral and cytotoxic activities very efficiently abolish the viral replicative cycle, permitting only mitotic expansion of provirus-carrying cells. Short term cultures of these infected cells initially indicated that viral expression protects against spontaneous apoptosis, suggesting that leukemia is a process of accumulation of long-lived cells. This conclusion was recently reconsidered following in vivo dynamic studies based on perfusions of nucleoside (bromodeoxyuridine) or fluorescent protein markers (CFSE). In sheep, the turnover rate of infected cells is increased, suggesting that a permanent clearance process is exerted by the immune system. Lymphocyte trafficking from and to the secondary lymphoid organs is a key component in the maintenance of cell homeostasis. The net outcome of the immune selective pressure is that only cells in which the virus is transcriptionally silenced survive and accumulate, ultimately leading to lymphocytosis. Activation of viral and/or cellular expression in this silent reservoir with deacetylase inhibitors causes the collapse of the proviral loads. In other words, modulation of viral expression appears to be curative in lymphocytic sheep, an approach that might also be efficient in patients infected with the related Human T-lymphotropic virus type 1. In summary, a dynamic interplay between BLV and the host immune response modulates a complex equilibrium between (i) viral expression driving (or) favoring proliferation and (ii) viral silencing preventing apoptosis. As conclusion, we propose a hypothetical model unifying all these mechanisms. [less ▲]Detailed reference viewed: 301 (46 ULg)
The cell envelope in Proteus vulgaris P 18 : Isolation and characterization of the peptidoglycan component
; ; et al
in Biochimica et Biophysica Acta - Biomembranes (1971), 223(3), 489-503
Degradation of heat-treated cells of Proteus vulgaris P 18 with a protease preparation from a soil Bacillus subtilis allowed the isolation of a cell envelope structural entity, 60-70 Å thick, consisting ... [more ▼]
Degradation of heat-treated cells of Proteus vulgaris P 18 with a protease preparation from a soil Bacillus subtilis allowed the isolation of a cell envelope structural entity, 60-70 Å thick, consisting of two electron-dense layers separated by a light one and composed, at least in part, of lipopolysaccharide O-antigen and of peptidoglycan. An enriched preparation, of which 55 % of the dry weight consisted of an intact peptidoglycan, was obtained through the sequential action upon lyophilized cells of hot aqueous sodium dodecyl sulfate and B. subtilis protease. Both Chalaropsis B endo-N-acetylmuramidase and Streptomyces DD carboxy-peptidase solubilized the enriched peptidoglycan preparation. The Chalaropsis enzyme completely degraded the glycan moiety into peptide-substituted disaccharide units. The DD carboxypeptidase hydrolyzed the D-alanyl-(D)-meso-diaminopimelic acid linkages involved in peptide cross-linking. In the intact peptidoglycan, about 35 % of the L-alanyl-γ-D-glutamyl-(L)-meso-diaminopimelic acid-(L)-D-alanine residues occurred as uncross-linked monomers, 40 % of them as peptide dimers and 16 % as peptide trimers. It is likely that not all the peptides retained the D-alanine residue at their C-termini. About half of the N-acetylmuramic acid residues in the glycan moiety are O-acetylated, a property which is compatible with the high lysozyme resistance exhibited by the P. vulgaris peptidoglycan. [less ▲]Detailed reference viewed: 30 (1 ULg)
Cell factories for the production of bioactive peptides from Bacillus subtilis and Pseudomonas.
; ; Thonart, Philippe et al
Poster (1997, April)Detailed reference viewed: 31 (0 ULg)
Cell Handling, Membrane-Binding Properties, And Membrane-Penetration Modeling Approaches Of Pivampicillin And Phthalimidomethylampicillin, Two Basic Esters Of Ampicillin, In Comparison With Chloroquine And Azithromycin
; ; et al
in Pharmaceutical Research (2003), 20(4), 624-31
PURPOSE: The purpose of this work was to examine and understand the cellular pharmacokinetics of two basic esters of ampicillin, pivaloyloxymethyl (PIVA) and phthalimidomethyl (PIMA), in comparison with ... [more ▼]
PURPOSE: The purpose of this work was to examine and understand the cellular pharmacokinetics of two basic esters of ampicillin, pivaloyloxymethyl (PIVA) and phthalimidomethyl (PIMA), in comparison with lysosomotropic drugs (chloroquine, azithromycin). METHODS: Cell culture studies (J774 macrophages) were undertaken to study uptake and release kinetics and to assess the influence of concentration, pH, proton ionophore (monensin), and MRP and P-gp inhibitors (probenecid, gemfibrozil, cyclosporin A, GF 120918). Equilibrium dialysis with liposomes were performed to directly asses the extent of drug binding to bilayers. Conformational analysis modeling of the drug penetration in bilayers was conducted to rationalize the experimental observations. RESULTS: PIVA and PIMA showed properties in almost complete contrast with those of chloroquine and azithromycin, i.e., fast apparent accumulation and fast release at 4 degrees C as well as at 37 degrees C, saturation of uptake (apparent Kd 40 microM), no influence of monensin, MRP, or P-gp inhibitors; tight binding to liposomes (Kd approx. 40 microM); and sharp increase in calculated free energy when forced in the hydrophobic domain. CONCLUSIONS: Although they are weak organic bases, PIVA and PIMA show none of the properties of lysosomotropic agents. We hypothesize that they remain locked onto the pericellular membrane and may never penetrate cells as such in significant amounts. [less ▲]Detailed reference viewed: 6 (0 ULg)
Cell invasion in the spheroid sprouting assay: a spatial organisation analysis adaptable to cell behaviour.
Blacher, Silvia ; Erpicum, Charlotte ; et al
in PLoS ONE (2014), 9(5), 97019
The endothelial cell spheroid assay provides a suitable in vitro model to study (lymph) angiogenesis and test pro- and anti-(lymph) angiogenic factors or drugs. Usually, the extent of cell invasion ... [more ▼]
The endothelial cell spheroid assay provides a suitable in vitro model to study (lymph) angiogenesis and test pro- and anti-(lymph) angiogenic factors or drugs. Usually, the extent of cell invasion, observed through optical microscopy, is measured. The present study proposes the spatial distribution of migrated cells as a new descriptor of the (lymph) angiogenic response. The utility of this novel method rests with its capacity to locally characterise spheroid structure, allowing not only the investigation of single and collective cell invasion but also the evolution of the spheroid core itself. Moreover, the proposed method can be applied to 2D-projected spheroid images obtained by optical microscopy, as well as to 3D images acquired by confocal microscopy. To validate the proposed methodology, endothelial cell invasion was evaluated under different experimental conditions. The results were compared with widely used global parameters. The comparison shows that our method prevents local spheroid modifications from being overlooked and leading to the possible misinterpretation of results. [less ▲]Detailed reference viewed: 47 (6 ULg)
Cell localization and redistribution of the 67 kD laminin receptor and alpha 6 beta 1 integrin subunits in response to laminin stimulation: an immunogold electron microscopy study.
; ; et al
in Cell Adhesion and Communication (1994), 2(3), 201-9
The 67 kDa laminin receptor (67LR), one of several cell surface laminin-binding proteins, is involved in the interactions between cancer cells and laminin during tumor invasion and metastasis. A 37 kDa ... [more ▼]
The 67 kDa laminin receptor (67LR), one of several cell surface laminin-binding proteins, is involved in the interactions between cancer cells and laminin during tumor invasion and metastasis. A 37 kDa polypeptide (37LRP), previously identified as the 67LR precursor, is abundantly present in the cytoplasm and has been implicated in polysome formation. To better understand the cellular localization of the 67LR and its precursor, transmission electron microscopic studies of human melanoma A2058 cells were carried out using immunogold labeling and a variety of antibodies: (a) affinity purified antibodies directed against 37LRP cDNA-derived synthetic peptides; (b) anti-67LR monoclonal antibodies raised against intact human small cell lung carcinoma cells; and (c) monoclonal antibodies against the subunits of the integrin laminin receptor, alpha 6 beta 1. Double-labeling immunocyto-chemistry revealed that anti-67LR monoclonal antibodies as well as anti-37 LRP antibodies recognized antigens that were localized in the cytoplasm in electron dense structures. As expected, cell membrane labeling was also observed. Surprisingly, alpha 6 and beta 1 integrin subunits were detected in the same cytoplasmic structures positive for the 67LR and the 37LRP. After addition of soluble laminin to A2058 cells in suspension, the number of labeled cytoplasmic structures increased especially in the vicinity of the plasma membrane, and were exported onto the cell surface. Neither fibronectin nor BSA induced such an effect. The data demonstrate that the 67 LR and the 37 LRP antibodies detect colocalized antigens that are in cytoplasmic structures with alpha 6 beta 1 integrin.(ABSTRACT TRUNCATED AT 250 WORDS) [less ▲]Detailed reference viewed: 17 (0 ULg)
Cell lysis during coccolithophorid blooms in the Northern Bay of Biscay
; Harlay, Jérôme ; et al
Poster (2009, January 25)
Phytoplankton cell lysis occurs in natural populations and is often associated with viral activity and zooplankton grazing. Cell lysis rates are expected to increase towards the decaying phase of the ... [more ▼]
Phytoplankton cell lysis occurs in natural populations and is often associated with viral activity and zooplankton grazing. Cell lysis rates are expected to increase towards the decaying phase of the bloom and may be associated with enhanced microbial activity and export of particulate matter to the seafloor. Their estimation was based on the measurement of esterase (a cytoplasmic enzyme) activity expected to appear in the water only after cell breakage. Field investigations, supported by remote sensing data, were conducted in recent years during late spring in the Northern Bay of Biscay, where frequent and recurrent coccolithophorid blooms are observed. Results on cell lysis rates determined in surface waters will be presented with relevant biogeochemical parameters (temperature, particulate organic and inorganic carbon, transparent exopolymer particles, nutrients, chlorophyll a) in order to investigate phytoplankton dynamics in relation to coccolithophorid development. The use of this parameter to characterize bloom termination, especially during coccolithophorid blooms will be discussed. [less ▲]Detailed reference viewed: 18 (0 ULg)
Cell membrane destructuration using cyclodextrins: effect on cholesterol content of endothelial cells
Castagne, Delphine ; Delattre, Luc ; Evrard, Brigitte et al
Conference (2007, October)Detailed reference viewed: 15 (0 ULg)
Cell membrane proteomic analysis identifies proteins differentially expressed in osteotropic human breast cancer cells.
Kischel, Philippe ; ; Dumont, Bruno et al
in Neoplasia : An International Journal for Oncology Research (2008), 10(9), 1014-20
Metastatic breast cancer cells are characterized by their high propensity to colonize the skeleton and form bone metastases, causing major morbidity and mortality. Identifying key proteins involved in the ... [more ▼]
Metastatic breast cancer cells are characterized by their high propensity to colonize the skeleton and form bone metastases, causing major morbidity and mortality. Identifying key proteins involved in the osteotropic phenotype would represent a major step toward the development of both new prognostic markers and new effective therapies. Cell surface proteins differentially expressed in cancer cells are preferred potential targets for antibody-based targeted therapies. In this study, using cell surface biotinylation and a mass spectrometric approach, we have compared the profile of accessible cell surface proteins between the human breast cancer cell line MDA-MB-231 and its highly osteotropic B02 subclone. This strategy allowed the identification of several proteins either up- or downregulated in the osteotropic cell line, and differential protein expressions were validated using antibody-based techniques. Class I HLAs were down-regulated in the bone metastatic variant, whereas alpha(v)beta(3) integrins, among others, were consistently up-regulated in this latter cell line. These results show that comprehensive profiling of the cell surface proteome of mother cancerous cell lines and derived organ-specific metastatic cell lines provides an effective approach for the identification of potential accessible marker proteins for both prognosis and antibody-based targeted therapies. [less ▲]Detailed reference viewed: 65 (9 ULg)
Cell Models Adapted to Real-Time Imaging of the Cytoskeleton Dynamics in Altered Gravity
Willems, Jérôme ; Deroanne, Christophe ; Colige, Alain et al
in Microgravity Science and Technology (2014), 26(4), 257-270
Spatial and temporal regulation of cell phenotype by mechanical forces is a growing field of research in health sciences since these stimuli influence cellular functions, such as proliferation, migration ... [more ▼]
Spatial and temporal regulation of cell phenotype by mechanical forces is a growing field of research in health sciences since these stimuli influence cellular functions, such as proliferation, migration, differentiation and gene expression. In the context of the Fluolive project selected by the European Space Agency and aiming at evaluating the impact of gravity alterations on the cell phenotype, we have developed new bone-derived cell lines adapted for live-cell imaging of the cytoskeleton. Osteoblastic cells derived from human osteosarcomas were used as experimental models. U2-OS and SaoS-2 cells stably expressing TagGFP2- β-actin and mCherry- α-tubulin were established and single-cell clonal cultures were characterized in terms of recombinant proteins production and localization, fluorescence intensity, cell proliferation and migration rates. Living fluorescently-tagged cell lines allow real-time fluorescence microscopy of the cytoskeleton dynamics without bleaching and without alteration of cell morphology. U2-OS and SaoS-2 TagGFP2- β-actin and mCherry- α-tubulin clones will be used to monitor the effect of mechanical forces in models of altered gravity on Earth and possibly on the ISS. © 2014, Springer Science+Business Media Dordrecht. [less ▲]Detailed reference viewed: 23 (3 ULg)
Cell proliferation in malignant melanoma: relationship with neoplastic progression.
PIERARD, Gérald
in ISRN Dermatology (2012), 2012(art.828146), 1-12Detailed reference viewed: 15 (3 ULg)
The cell separation step of bacterial cell division
Kerff, Frédéric
Scientific conference (2013, November 26)Detailed reference viewed: 14 (1 ULg)
Cell specific expression of Hsp70 in neurons and glia of the rat hippocampus after hyperthermia and kainic acid-induced seizure activity.
; ; Plumier, Jean-Christophe et al
in Molecular Brain Research (1999), 71(2), 265-78
In this study we investigated the time course, cell-type and stress-specific expression of hsp70 mRNA and Hsp70 protein in glial cells and neurons in the rat brain following heat shock treatment and ... [more ▼]
In this study we investigated the time course, cell-type and stress-specific expression of hsp70 mRNA and Hsp70 protein in glial cells and neurons in the rat brain following heat shock treatment and kainic acid-induced status epilepticus. Transcripts for hsp70 were detected in hippocampal homogenates from 1.5 to 6 h following hyperthermia and from 3 to 24 h following kainic acid-induced seizures. In situ hybridization revealed hsp70 mRNA to be region specific and time-dependent following hyperthermia and kainic acid-induced seizures. Western analysis indicated that Hsp70 reached maximal levels at 3 h after hyperthermia and 12 h after kainic acid-induced seizures. Immunohistochemistry revealed low level expression of Hsp70 protein in dentate granule cells at 1.5 and 3 h after hyperthermia. No Hsp70 protein was detected in neurons of the pyramidal cell layer or dentate hilus at any time following hyperthermia. Small Hsp70-immunoreactive cells were detected throughout the hippocampus following hyperthermia that, based on cell size, distribution, and double-labeling with vimentin, were considered to be glia. In contrast, high levels of Hsp70 protein were detected in neurons of the pyramidal cell layer and dentate hilus at 24 h after seizure-inducing kainic acid injection. These results suggest that expression of Hsp70 protein is cell-specific depending on the stressor. In addition, finding high levels of Hsp70 mRNA in the dentate granule cells after hyperthermia, but little or no Hsp70 protein, suggests that the synthesis of the protein is also regulated at the post-transcriptional level following hyperthermia. [less ▲]Detailed reference viewed: 34 (2 ULg)


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Foyles War
One of our section members has occasionally supplied historic period motorcycles for TV drama and films and the old cobbled streets of Liverpool have often found themselves the setting for TV advertisements and drama. Colin supplied a Norton Dominator for the John Lennon Liverpool setting of "Nowhere Boy", and has been asked to supply period motorcycles for a new project, part of which is to be filmed on the East coast.
One such, popular series that Colin and his bikes have appeared in is ITV's Foyles War where filming began in early 2014 in Liverpool, in and around the old cobbled streets, namely Faulkner Street, which was also the setting of the interbellum period (time between wars) of the 2008 award winning revival advertisement "Go on Lad" promoting Hovis.
Colin took along his 1928 AJS model K1 and was dressed up as a deliveryman for one of the shots featured in the Liverpool filming of Foyles War in early 2014.
The bike featured is a 1928, 799c V twin manufactured by AJS (A J Stevens) and its acquisition many years ago by Colin is rather interesting. Colin purchased the bike from a Fleetwood trawler man, it had belonged to the trawler man's father and when Colin arrived he didn't find it in a garage – it was actually in the mans bedroom – in pieces!
There were a lot of parts missing which, obviously due to the bikes age were difficult to source, although with persistence they were all eventually located by Colin and the later added feature of the sidecar was eventually found in the depths of the Keilder forest where it was being used as an axe store! The bike now has a new lease of life and, like its owner, has become a real star. You can see more of Colin's film career in our "Girls Night Out" article here.
Here are a few more photos of the sets and vehicles used in the filming of Foyles War in Liverpool.
Michael W George


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Roane State Community College biostatistics classes
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Learn about Roane State Community College biostatistics classes, and registering in a elective technical programs. Which college degrees give you the best chances of finding employment after graduation? Completion of an accredited engineering program can qualify you to enter the workforce as a paid intern immediately. Concentrating on Roane State Community College biostatistics classes, for example, you may gain an advantage in job recruiting on-campus. Check with the biotechnology department for course registration deadlines, and mechanical engineering program requirements. Please use the form above to request admissions info for Roane State Community College.
Biotechnology uses the DNA of living organisms to bioengineer new products artificially. Depending on the tools and applications, it often overlaps with the related fields of biomedicine, pharmacology, and genetic engineering. Modern biotech methods are used to manufacture existing medicines more easily, in order to treat human diseases. Further, biotechnology has enabled emerging techniques like gene therapy. Stem cell research offers new hope in the areas of cancer treatment as well as life extension. The regulation of genetic engineering aims to assess and manage the risks of developing genetically modified organisms (GMO), including commercial food crops and fish.
At the smallest scale, mechanical engineering becomes nanotechnology, one goal of which is to build molecules and materials via microsynthesis. Current areas of mechanical engineering research in biotech and nanotechnology include nanofilters, nanofilms, and nanostructures such as microtubules. Self-assembly of these structures may lead to new breakthroughs in artificial tissue generation. Micron-scale mechanical components such as springs, gears, and heat-transfer devices are fabricated from a variety of substrate materials such as silicon, glass and polymers like SU8. Biomechanics applies traditional engineering methodology to the analysis of biological systems. By copying the efficiency of nature, the study of biomechanics aids in creating prosthetic limbs and artificial organs for medical application. Beyond major requirements within the biotechnology department, you may consider Emory and Henry College research methods as an adjunct to your primary course of study.
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text:
"So You Think You Can Dance" has been great for Utah dancers. Not only has the show come to town for four auditions, but Utahns have had a lot of success on Fox's reality/competition series.
In fact, Utah has been overrepresented. Locals have made the finals in six of the eight seasons, including one in Season 5, two in Season 2, three in Season 6 and four in Season 4. Utahn Sabra Johnson won the title of "America's favorite dancer" in Season 3. The state's most recent finalist was Tadd Gadduang in the 2011 season.
Yet not everyone in the profession is enamored of the dance reality show. At the end of last season, The New York Times gathered a group of professional dancers to evaluate the show. In writer Elaine Stuart's story, they mocked the judges and the dancers, called the competition aspect "obscene," "cringed at the excessive kicks, splits and spins" and insisted, "These dancers wouldn't stand up to dancers in New York."


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"My wedding stationery arrived last week and I'm delighted with everything, they're beautiful and the quality is excellent. I have had comments from the guests saying how pretty they are!"
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text:
Thousands upon thousands have been served at Mavericks Steak & Cocktails, many of them write letters or leave comments. Here are just a few of the many comments and reviews that we have received from our happy customers.
Pros: great atmosphere & food variety
Cons: busy on weekend nights, so get there early
Maverick's is a great place to eat with a good variety of foods to choose from.
We were there for a family member's 50th birthday, they even brought him a huge chocolate cake sundae that we all shared before leaving. Very good location, with easy access in and out from a main road. Prices are very reasonable with the amount of food you get. Will enjoy it many more times I am sure!
When you go in there they are so helpful and the food is to die for so if you are even in town check them out. I'm sure you will love them like I do.


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Stats plugin
For some reason on a non wordpress site, I'm unable to connect to/with FTP, which is why I'm using such an old version. I need a stats plugin, but one that activates automatically as I cant connect to FTP. Are there any out there? My site is http://whatthe.blogetery.com/
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pictures from GarnetHill.com
I want this dress. In pink and/or in blue. (Usually I just want pink, so I must really like it.)
Long, fan-blown model hair would be nice, too.
Another trend I love: ruffles. So feminine and sweet, ruffles upgrade clothes from basic to lovely.
Let's accessorize another imaginary pink outfit:
Let's start with some Vera Bradley in Folkloric.
Purple punch is on sale, so we should add some to our accessories.
How about a purple headband to go with our new purple bag?
Add a crisp watch. . .
. . .and some preppy little flats. . .
and we have spent more imaginary money than we have to spend (imaginarily).
I am in the mood for (and need) some new clothes, but since I have to pay for my pancreas, I am too poor - no- economically minded to buy such pretty things. In the meantime, I just have to pretend-shop. Thanks for coming along with me!