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The following information on the double rifle comes from Rifles and Rifle Shooting by Charles Askins. Rifles & Rifle Shooting is also available to purchase in print.
The double express rifle has never been in much demand in this country, but any American who has occasion to shoot in India or Africa will find it wise to include one or more of these weapons in his armory. Men who hunt game of the nature of elephants, rhinos, lions, and tigers, have long pinned their faith to the double rifle, and we have no grounds for disputing their good judgment. Perhaps in course of time some form of magazine rifle will replace the double express, but up to the present date no other weapon has been found quite so deadly and reliable upon a charging beast at close quarters. More emphatically is this true where the English manner of stalking is followed, with the sportsman carrying one rifle and his shikari the second. If any rifle ever supersedes the double it seems now that it must be an automatic.
The highly finished English double rifle has long been considered the finest example of the art of gun building. Beyond question it requires more gunsmith's skill to put together two rifled tubes and insure that they will shoot to the same center at all distances from short range to the maximum than to construct any other style of gun. Not every double rifle will do this either, and the sportsman who finds himself in need of one of these arms should purchase only from the very best maker, and even then should carefully target and test his weapon.
The trouble with the double rifle is that both barrels may not shoot on exactly the same elevation, or they may shoot apart, or cross their missiles. Indeed it is quite impossible to make a double rifle quite as accurate at all ranges as a single barrel, but for practical purposes, at sporting ranges, it is just as effective.
There is no disputing the claim that a double hammerless, self-ejecting rifle of high grade is a splendid weapon. It is quicker with its two shots than any repeating rifle with the exception of the automatic; there is no such thing as balking or jamming it for the two shots it contains, whatever the excitement, and the arm is chambered for the most powerful sporting cartridges in use. Rifles of this description, when constructed of moderate weights to use medium cartridges, should have much the feel and balance of a shotgun which especially fits them for the sort of instinctive snap work that must sometimes stop a charging beast.
A double rifle can be obtained suitable for shooting any species of game from rooks and rabbits to elephants, in weights from five pounds to twenty. If it were worth while to purchase one of these weapons for American shooting, the .333 as made by Jeffery, using a 250 grain bullet driven with a velocity of 2,600 feet should certainly prove a killer. This cartridge is more powerful than anything we have and might appeal to the man who is looking for striking force and deadliness.
For service in India and Africa the double rifle can be obtained in various bores and cartridges. Some of the best liked are the .375, .400, .404, .450, .475, .500, .577, and .600 cordite. The latter is charged with one hundred grains of powder and a nine hundred grain bullet and is warranted to kill anything before it—perhaps behind as well.
No less an authority than W. W. Greener maintains that the recoil of these great rifles is unbearable, and that they never should have been built in such calibers. Probably the best double rifles for African game would be the .333, .400, .450, and .475. They appear to be quite powerful enough for elephants, have velocities and trajectories equal to the ordinary military small bores, and the recoil is to be preferred to the swing of an elephant's trunk.
In purchasing a double rifle economy should not be considered. A first rate rifle can hardly be secured under three hundred dollars and five hundred might well be expended for one. These rifles are made after the same design as the ordinary double shotgun by some builders, hammerless, ejector locks, top lever, and single trigger, where desired. The most favored mechanism for the more powerful cartridges, however, is the under lever, in which the lever which withdraws the lug bolts lies under the guard. It is stated that the under lever has ten times the withdrawing force at the top and that it entirely obviates jams from the tremendous pressure which sometimes occurs and disables the gun temporarily where the top lever is in use. The one excuse for the great double express rifle is its danger and it would not do to add to this by a mechanism that might possibly render the weapon useless in a time of peril.
The Shotgun and Rifle
Double barrel guns with one barrel for shot and the other rifled have never been manufactured in America so far as the writer is aware. Some of them have been imported, chiefly in the hands of immigrants from Germany and France. I think not a great many of them are now being made even in Europe.
This sort of weapon has its uses in a country of mixed small and large game. It is well adapted to driven deer shooting after the German fashion especially for small deer like the roe. One barrel loaded with buckshot could then be used for running animals and the rifle cartridge for still shots. The arm once saw a certain amount of service in the south where chasing deer with hounds, heading them off, and shooting from horseback was the accepted style. It is a handy gun for mixed turkey and small game, but for this purpose is not so good as the next model we have to consider, the three-barrel.
The Three Barrel Gun
The three-barrel gun as now constructed has two shotgun barrels lying side by side as usual with a rifle barrel beneath. But two locks are used to fire the three barrels, the shift being made from one of the shot barrels to the rifle by means of a lever. These weapons are still being built in this country, also numbers of them are imported.
Formerly they were manufactured in different styles, some with the rifle barrel on top in place of the top rib, and the late D. Kirkwood, of Boston, has made them with four barrels two shot and two rifle. He was an old time expert gunsmith of a kind now becoming rare.
The three-barrel gun is an attractive arm for certain purposes. For the mixed shooting such as can be found in Florida or Texas, principally quail and wildfowl but occasionally deer or turkey, it is a combination not to be excelled in a single weapon. It is, of course, a good double shotgun with the added advantage of an accurate rifle always at the sportsman's command. In a country like Texas, Mexico, or Central America where the game may be turkey, deer, bobcats, wildfowl, or quail, I do not know of a more useful weapon than the three barrel.
These arms are now being chambered for modern high power cartridges such as the .25-35, .30-30, .32-40, and .38-55, as well as for lighter ammunition, including the .25-20-86. Probably the .30-30 is the most favored cartridge, with 16 or 20 gauge shot barrels, the entire arm to weigh not exceeding 7 1/4, pounds.
Even the man who shoots in a well settled country can get quite a bit of sport out of his three barrel that he would otherwise miss. With it crows, hawks, foxes, and woodchucks can be killed, while it is a more sportsmanlike weapon for shooting rabbits and squirrels than any shotgun. Cracking the head of a squirrel in the top of a hundred foot hickory is a worthy feat, but shooting him with shot is mere pot hunting.
The use of a three-barreled arm is pretty well confined to America, North and South. In Europe a different weapon replaces it, the muzzle rifled shotgun.
As its name implies, this is a shotgun with its muzzle having a shallow rifling, calculated to spin a cylindrical, pointed bullet of moderate length. Usually the ball is hollow at point or base, sometimes both, so as to permit the greatest length of missile without undue weight.
The most popular sizes of "ball-and-shot-guns " are the 20 and 28 gauges. The 28 drives a rifle bullet of 290 grains, the 20, one of 380. The velocities are from fourteen to sixteen hundred feet, and the striking force from seventeen hundred to twenty-four hundred foot pounds. The accuracy is said to be nearly equal to a double rifle at ranges up to two hundred yards. The arm weighs in these lighter gauges from 5 1/4 to 7 pounds. With a shot charge the weapon is about as effective as any other cylinder bored gun.
Arms of this description would no doubt be very convenient where the sportsman was obliged to travel light, as in long canoe voyages, where the limit of weight was one gun, and the game of a mixed character. As a rifle the power would be sufficient for anything up to moose.
The rifled shotgun is also made in heavier bores, 16, 12, 10, and 8, with charges running as high as ten drams of powder and a thousand grain ball. While such weapons are used in India for cover shooting there is little about them to attract an American sportsman.


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Essays on fathers rights
Patriotic Essays. Presented here are a some essays that I have assembled for all of you with a patriotic heart. If you have a favorite essay that you would like to. Northwest Nazarene University. Founded in 1913, the university now serves over 1,900 undergraduate and graduate students, more than 10,000 continuing education.
Essays and criticism on William Shakespeare - Critical Essays Custody rights for gay men and lesbians have improved since the 1970s, but differential treatment persists. The Federalist (later known as The Federalist Papers) is a collection of 85 articles and essays written (under the pseudonym Publius) by Alexander Hamilton, James.
Essays on fathers rights
Fathers' Fight. What every mother should know about the fathers' rights movement. By Sarah Werthan Buttenwieser | print | I grew up as a child of divorce, shuttled. State law rather than federal law governed women's rights in the early republic. The authority of state law meant that much depended upon where a woman lived and.
Featured Exhibits. Amending America Highlighting the remarkably American story of how we have amended, or attempted to amend, the Constitution in order to form a. Non-Canonical Literature Disclaimer. Can any who spend several years in those seats of learning, be excused if they do not add to that reading of the Fathers the most.
The Founding Fathers on Arms "Firearms stand next in importance to the constitution itself. They are the American people's liberty teeth and keystone under. Fathers For Good Father's Day index page. My father helped us see better physically and spiritually. While I was still in Rome on graduate studies in Scripture in. You are in a correct place to find collection of sample essays and English speeches for kids. All these sample essays will help kids to improve essay writing skill.
Welcome to our Federalist Papers e-text. The Federalist Papers were written and published during the years 1787 and 1788 in several New York State newspapers to.

subset: commoncrawl


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Hey guys i want to hook this ignition switch up to my wr450 and want to know how i would do it as my manual i have doesnt say what wire is what so i am stuffed.
That is the switch i am going to buy as i cant seem to find a wr450 one.
I just got my bike registered and i have a 2 wire toggle on/off switch hooked up so anyone can jump on and ride off on my bike while i am in the shop.
Thanks guys hope to have someone help me out.Edited by SEDUCE


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Free download AVS Disc Creator for Windows
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Different markets have different types of price behavior and this carries over to the volatility of their option markets. Join Russell and Jill as they discuss the behavior of volatility for the S&P 500, emerging markets, oil and gold based on the behavior of the underlying markets.
When: Thursday, April 19
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You will be able to pre-register but the presentation WILL NOT be live until 15 minutes before the webinar.
You will be required to provide your name, email address and phone number to gain access to the presentation. Course material and replay link will be made available Monday, April 23.
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text:
"Arya 2" releasing on November 27
Allu Arjun's "Arya-2" not exactly a sequel to the earlier successful film "Arya" is jointly produced by V Aditya Babu and Bhogavalli Prasad under the banner of Aditya Arts and directed by Sukumar, who helmed the original.
Kajal Agarwal, who earned fame with "Magadheera" plays the female lead while Navadeep and Shraddha Das play parallel roles. The music for the film has been composed by Devi Sri Prasad.
Allu Arjun looks in a more stylistic personality in this film. The promos of this film are very promising and alluring. Let us hope the film lives up to the hype.


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It's Never Finished
If they'd just give us time to finish it...
It's never finished.
Read that again. You're not writing TeX. You may have a bold vision for how this API will develop, and that's fantastic. But you still need to deliver on that vision one step at a time, and every iteration needs to be useful and maintainable.
"This will be painful in the short term, but it'll get better when we deliver version 2."
— Developer Destined for Disappointment
It's hard to find the time to deliver version 2 if you spend all your time putting out the fires of version 1. Always assume that the next milestone you deliver is going to be the last.
Sometimes that's a tradeoff you're willing to make. Sometimes the market opportunity is too important and the company ceases to exist if you don't cut that corner and create a maintenance nightmare. But understand the costs and don't kid yourself that you'll get time to go back and fix it. If your company is on a knife's edge when you make that tradeoff, it's quite likely that you'll still be in crisis mode next month.
The best you can do is question very hard whether you really need that one feature, and whether there are any more maintainable alternatives that are almost as good.


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To test the hypothesis that cortisol and epinephrine have direct regulatory roles in muscle glycogen metabolism and to determine what those roles might be, we developed an in vitro white muscle slice preparation from rainbow trout (Oncorhynchus mykiss Walbaum). In the absence of hormones, glycogen-depleted muscle slices obtained from exercised trout were capable of significant glycogen synthesis, and the amount of glycogen synthesized was inversely correlated with the initial postexercise glycogen content. When postexercise glycogen levels were <5 μmol/g, about 4.3 μmol/g of glycogen were synthesized, but when postexercise glycogen levels were >5 μmol/g, only about 1.7 μmol/g of glycogen was synthesized. This difference in the amount of glycogen synthesized was reflected in the degree of activation of glycogen synthase. Postexercise glycogen content also influenced the response of the muscle to 10−8 M epinephrine and 10−8 M dexamethasone (a glucocorticoid analog). At high glycogen levels (>5 μmol/g), epinephrine and dexamethasone stimulated glycogen phosphorylase activity and net glycogenolysis, whereas at low (<5 μmol/g) glycogen levels, glycogenesis and activation of glycogen synthase activity prevailed. These data clearly indicate not only is trout muscle capable of in situ glycogenesis, but the amount of glycogen synthesized is a function of initial glycogen content. Furthermore, whereas dexamethasone and epinephrine directly stimulate muscle glycogen metabolism, the net effect is dependent on initial glycogen content.
- cortisol
- glycogen phosphorylase
- glycogen synthase
most of the muscle mass in teleost fish is white or "anaerobic" muscle, consisting of fast-twitch, glycolytic muscle fibers. These fibers are responsible for powering more intensive bursts of exercise and use the breakdown of glycogen (glycogenolysis) as an energy source (24, 25, 30). During high-speed swimming in trout, glycogenolysis is rapidly activated in white muscle (42) and, when exhausted, reserves of glycogen are reduced and lactate accumulates.
Whereas there is increasing evidence that muscle glycogen is resynthesized in situ with lactate as the main substrate (32, 39, 51), little is known how this process is regulated. Glycogen phosphorylase (GPase, E.C. 220.127.116.11) and glycogen synthase (GSase, E.C. 220.127.116.11) are likely to be key control points. The rate of net muscle glycogenesis is a function of the relative activities of GSase and GPase. The activity of these enzymes is regulated by a complex phosphorylation-dephosphorylation cycle. Both GPase and GSase exist in two interconvertible forms, active (a) and inactive (b) (nominally). Phosphorylation of GPase by phosphorylase kinase, which converts GPase b to GPase a, leads to an increase in activity, whereas phosphorylation of GSase, converting GSase I (active form) to GSase D (inactive form), decreases its activity (see Refs. 11 and 23 for reviews). These transformations are regulated hormonally (e.g., epinephrine and glucagon) via cAMP-mediated activation of kinases and by contraction-induced changes in intracellular Ca2+ (11, 14, 23). Net mobilization of glycogen then depends not only on activation of GPase but also on the concomitant inhibition of GSase. In trout muscle at the end of 5 min of exhaustive exercise, there is a two- to threefold increase in GPase a activity (42) while GSase activity remains at or below basal levels (31).
The rate-limiting step for glycogenesis in fish muscle is less clear. In mammalian muscle, it believed that the control points for glycogenesis are shared between glucose transport and GSase (2, 15, 23). However, others (10, 45, 46) argue that the activities of hexokinase and glucose transport control the glycogenic rate, with the activity of GSase adapting to accommodate the change in flux. There are reasons to believe that regulation of glycogen synthesis is different in fish muscle from that in mammalian muscle. A major difference between trout and mammalian muscle lies in their abilities to utilize blood-borne glucose as a glycogenic substrate. Trout white muscle poorly utilizes blood glucose (39, 51) and has low hexokinase activity (0.06 μmol·min−1·g−1 at 15°C; 26, 47) compared with human skeletal muscle of mixed fiber type (∼3 μmol·min−1·g−1 at 37°C; 7). Furthermore, whereas glucose transporters have been found in trout muscle membranes, their expression is extremely low and their physiological role unclear (9, 28, 48). Given this low glucose use by trout white muscle, neither glucose transport nor hexokinase activities are likely important regulatory sites for glycogenesis in trout muscle, rather regulation of GSase activity is likely more important.
Muscle GSase activity, like that of GPase, is sensitive to hormonal stimulation. For example, Ottolenghi et al. (37) showed that in catfish (Ictalurus punctatus) muscle, epinephrine stimulates GPase a activity and net glycogenolysis while inhibiting GSase I activity. Epinephrine is presumably acting via a β-adrenergic receptor-mediated cAMP cascade (14, 29). Cortisol has also been implicated as a regulatory agent for glycogen synthesis, because, when elevated, cortisol inhibits GSase I activity and net glycogen synthesis (31). After glycogen-depleting exercise, there is a period during which both epinephrine and cortisol are elevated, and during this period there is no net glycogen synthesis. It is not until levels of these hormones return toward resting levels that muscle glycogen resynthesis begins (38). Therefore, the hypothesis has been put forward suggesting that cortisol, alone or in conjunction with catecholamines, exerts an inhibitory effect on glycogenesis via inhibition of GSase activity.
To test the hypothesis that cortisol and epinephrine are acting directly at the muscle to regulate glycogen metabolism, an in vitro white muscle slice preparation has been developed. In vivo studies (13, 31) have demonstrated a regulatory role of cortisol in muscle glycogen metabolism, but it is not clear whether the effect is directly at the muscle or an indirect effect. Furthermore, it is not clear whether cortisol is acting alone or in conjunction with catecholamines, in a permissive-type role (e.g., 19). With the use of an isolated white muscle tissue slice preparation, the direct effects of cortisol, epinephrine, and the two in combination at the level of the muscle can be readily observed. Because a white muscle slice preparation from rainbow trout has not been used in this context before, it was necessary to ensure that the preparation was viable and responsive to hormones. To ascertain the former, O2 consumption and metabolite levels were monitored throughout the incubation period, and hormonal responsiveness was determined by incubating the slices with glucagon, which is known to act on fish muscle (34).
Experimental Animals
Male and female rainbow trout (Oncorhynchus mykiss Walbaum; 150–250 g) were purchased from Rainbow Springs Trout Farm (Thamesford, Ontario, Canada) throughout the year. The trout were held in a large circular tank (1,000 liters) and provided with a continuous flow of dechlorinated London, Ontario city water kept at 14 ± 1°C on a 12:12-h light-dark light cycle. The fish were fed daily to satiation with commercial trout pellets. Twenty-four hours before experimentation, fish were isolated in black, acrylic, 4-liter boxes continuously aerated and supplied with dechlorinated London, Ontario city water at 14 ± 1°C. Isolation of fish in individual boxes before experimentation facilitated sampling of fish at rest and transfer of individuals to the swimming tank. During this holding period fish were not fed. A total of 50 fish were used in these experiments. The University of Western Ontario Animal Use Subcommittee in accordance with the Canada Council on Animal Care guidelines approved these experiments.
Experimental Protocol
On the day of the experiment, fish were individually transferred from their holding boxes to a 300-liter circular tank and manually chased for 5 min, at which point they were unresponsive to further stimuli and considered exhausted. This method of exhausting fish has been used extensively and results in consistent metabolic disturbances (25, 30). Immediately before the tissue sampling, fish were killed with an overdose of tricaine methane sulfonate (0.1 g/l, MS-222, Syndel Labs, Vancouver, Canada) buffered to pH 7.8 with NaHCO3. Resting fish were treated in a similar manner, except they were not exercised.
A block of muscle tissue (∼1.5 cm3) was rapidly excised from the dorsal epaxial muscle mass and placed in ice-cold modified Cortland's saline. The saline contained (in mM) 124 NaCl, 5.1 KCl, 1.9 MgSO4, 2.9 Na2HPO4, 11.9 NaHCO3, 1.4 MgCl2, 5 glucose, 5 l-lactate, and 10 sodium pyruvate; pH was adjusted to 7.8 with 1 mM NaOH and equilibrated with 99.5% O2-0.5% CO2 (22). The muscle block was cut along a longitudinal axis using a Stadie-Riggs microtome (Thomas Scientific; Swedesboro, NJ) into slices with an average thickness of 1.32 ± 0.02 mm (n = 21) and mass of 0.235 ± 0.011 g (n = 27). Immediately after slices were obtained, one slice from each fish was blotted dry and frozen between aluminum blocks precooled with liquid nitrogen. This slice was used to determine the metabolic status of the muscle at the time of sampling and referred to as the "time 0 slice." The remaining slices were incubated individually in 3.5 ml of modified Cortland's saline (as above) in a 25-ml Erlenmeyer flask and continuously aerated with humidified 99.5% O2-0.5% CO2 for 1 h at 15°C in a shaking water bath. At the end of 1 h, tissue slices were removed, blotted dry, and freeze-clamped as described. Frozen tissue was then ground into a fine powder with a pestle and mortar cooled with liquid nitrogen, and the powder was stored at −80°C until analyzed for metabolites and enzyme activities.
Experimental Series
For each experimental trial, up to 10 muscle slices from a single fish were incubated as described, with each treatment carried out in duplicate. For each experimental series, tissue slices were obtained from 8 to 10 different fish. Therefore, in the figures, n refers to the total number of fish from which tissue slices were obtained.
Preparation viability.
To determine the viability of the tissue slices over time, oxygen consumption was measured at 10-min intervals for 60 min with a Gilson respirometer (Gilson Medical Electronics; Middleton, WI) at 15°C. In addition, at time 0 and after 60 min of incubation, slices were blotted dry and freeze-clamped for measurement of lactate, ATP, phosphocreatine (PCr), and glycogen content.
Effects of glucagon, epinephrine, cortisol, and dexamethasone on muscle glycogen metabolism.
Tissue slices obtained from fish either at rest or immediately following exercise were incubated in modified Cortland's saline containing a final concentration of either 10−8 M epinephrine bitartrate, 10−8 M dexamethasone-21-phosphate, 10−8 M bovine glucagon (Sigma Chemical; Mississauga, ON, Canada), or 10−8 M cortisol (hydrocortisone, gift of Dr. J. P. Wiebe, Dept. of Biology, University of Western Ontario) dissolved in 2% ethanol. Control slices were incubated in saline or saline containing 2% ethanol. Slices incubated in 2% ethanol were not different from those incubated in saline alone, thus all control values were pooled.
At the end of 60 min, the slices were removed, blotted to remove excess saline, and frozen between aluminum blocks precooled with liquid nitrogen and stored at −80°C until later analyzed for glycogen, GSase, and GPase activities.
Analytic Techniques
Muscle glycogen content was assayed by isolating the glycogen (20) and measuring the free glucose after digestion of the glycogen with amyloglucosidase (Worthington Biochemical; Lakewood, NJ; 4). In our laboratory, we typically recover 95–100% of the glycogen with this method. Muscle concentrations of lactate, ATP, and PCr were measured in ∼100 mg of muscle ground to a fine powder in a liquid N2-cooled mortar and vigorously resuspended in 1 ml 8% HClO4. Lactate, ATP, and PCr were then measured in the neutralized supernatant (4).
The relative activities of GPase were measured spectrophotometrically by using a modification of the method outlined by Storey (47). GPase exists in two forms: the more active a and the less active b (47). To determine the amount of GPase a, 40–50 mg of powdered tissue were homogenized on ice for 3 × 10-s bursts with a Tissue Tearor (Biospec Products; Bartlesville, OK) set to speed 6. The homogenization buffer contained (in mM) 50 imidazole HCl (pH 7.0), 100 NaF, 5 EGTA, 2 EDTA, 30 2-mercaptoethanol, and 0.1 phenylmethylsulfonylfluoride. Aliquots of 5 μl were then added to wells in a microplate reader containing 245 μl of assay buffer consisting of 50 mM potassium phosphate (pH 7.0), 2 mg/ml glycogen (oyster muscle; dialyzed), 0.4 mM NAD, 10 μM glucose 1,6 biphosphate, 0.25 mM EDTA, 15 mM MgCl2, 0.5 U glucose-6-phosphate dehydrogenase (Boehringer-Mannheim Chemical, Laval, PQ, Canada), and 1.4 units phosphoglucomutase (Worthington Biochemical). The wells were mixed and allowed to stand for 5 min at 20°C, and then absorbance at 340 nm was measured for 5 min with a Spectromax 340 PC microplate reader. Total activity a + b was measured by adding 4 μl of 5′-AMP to bring the well to a final concentration of 1.6 mM 5′-AMP, and the absorbance was measured for 5 min at 20°C. One unit of enzyme activity is defined as the amount of enzyme that produces 1 μmol of product per minute at 20°C. GPase activity is expressed as the ratio of (a/a + b ) × 100.
GSase also exists in two forms, the more active I and the less active D, and their activities were measured by the method of Schallin-Jantti et al. (44). The assay couples the conversion of UDP-glucose (UDP-G) to UDP and glycogen with the oxidation of NADH catalyzed by two enzymatic steps that involve pyruvate kinase and lactate dehydrogenase. To determine GSase activity, 40–50 mg of frozen, powdered tissue were suspended in buffer containing (in mM) 50 KF, 50 Tris (pH 7.8), 0.5 dithiothrietol, 1.0 EDTA, and 2.0 MgCl2. The homogenate was mixed vigorously (2 min on a vortex mixer set to maximum speed) and briefly centrifuged (1 min at 10,000 g), and the supernatant was assayed for GSase activity. GSase activity measured in samples processed this way was less variable and more consistent with previously published values compared with tissues processed with a mechanical tissue homogenizer. A 100-μl aliquot of the homogenate was added to 820 μl of assay mixture containing 70 mM KCl, 50 mM Tris (pH 7.8), 7 mM MgCl2, 10 mM glycogen, 5 mM phosphoenolpyruvate 0.42 mM NADH, 50 units pyruvate kinase, and 25 units lactate dehydrogenase (both enzymes from Worthington Biochemical). Activity was measured in either the presence or absence of glucose-6-phosphate (GSase I activity), 0.1 mM (GSase fractional activity), or 10 mM (GSase I + D activity) glucose-6-phosphate. To start the reaction, UDP-G was added to a final concentration of 20 mM. Absorbance at 340 nm was measured at 20°C for 20 min with a Shimadzu UV-160 recording spectrophotometer. GSase activity is expressed as the ratio of [I/(I + D)] × 100.
Unless otherwise stated, all biochemicals were purchased from Sigma Chemical. All other reagents were acquired from local suppliers and were of the highest available purity.
Statistical Analyses
Values presented are means ± SE (n). A repeated measures ANOVA was performed on the oxygen consumption data to assess any differences through time. A series of one-way ANOVAs was used for comparison between treatments, followed by a Tukey-Kramer honest significant difference (HSD) analysis. All statistical analyses were done using the software JMP (SAS Institute, Cary, NC), and significant differences were accepted when P < 0.05.
Tissue Viability
Tissue slices obtained from both resting and exercised fish remained viable for the entire 60-min incubation period, as indicated by the maintenance of oxygen consumption rates (Fig. 1) and volatile metabolites such as ATP and PCr (Fig. 2). Those slices taken from exercised fish were capable of enhanced oxygen consumption compared with slices from resting fish (Fig. 1) for the first 40 min of incubation. From 40 min onward, the oxygen consumption of muscle slices from exercised fish was not different from the slices obtained from fish at rest.
A second indicator of the viability of the muscle slices is seen in Fig. 2, which demonstrates that not only are ATP, PCr, glycogen, and lactate levels unchanged over the 60-min period in slices from resting fish (Fig. 2A), but more importantly, these metabolites, which were altered as a result of exercise, show recovery (Fig. 2B). Glycogen, ATP, and PCr are all lower at time 0 than at 60 min in exercised fish, whereas lactate levels are reduced at 60 min compared with time 0 levels. This indicates that the muscle slice is a metabolically viable preparation and that trout white muscle is capable of significant glycogenesis.
Hormonal Influences on Muscle Glycogen Metabolism
In the absence of any hormonal stimulation, muscle glycogen levels in muscle slices obtained from resting fish did not change over the 60-min incubation period. Incubation of slices in 10−8 M bovine glucagon stimulated glycogenolysis, reducing glycogen by ∼50% (Fig. 3A). Tissue slices obtained from fish postexercise synthesized glycogen in the absence of any hormone (control), with a net synthesis of about 3.4 μmol/g (Fig. 3B). Incubation of muscle slices from exercised fish with 10−8 M glucagon inhibited glycogenesis compared with control (i.e., no treatment).
In slices obtained from resting fish in which glycogen levels at time 0 were between 7 and 10 μmol/g, 10−8 M dexamethasone-21-phosphate, 10−8 M cortisol, and 10−8 M epinephrine all stimulated net glycogenolysis, with an average decline in glycogen of about 55–60% (Fig. 4A). There was no further stimulation of glycogenolysis in muscle slices incubated with 10−8 M dexamethasone and 10−8 M epinephrine in combination. Coincident with the stimulation of glycogenolysis was a significant threefold increase in the proportion of GPase in the active form (Fig. 4B) and a reduction in the active proportion of GSase (Fig. 4C). The maximal total activities of GPase (0.71 ± 0.05 U/g wet tissue, n = 8) and GSase (1.19 ± 0.07, U/g wet tissue, n = 8) were unaffected by any treatment.
The response of muscle slices isolated from exercised fish differed from that of resting fish and appears to be related to endogenous glycogen levels at time 0. Even though all slices were obtained from fish that had been exercised, the extent of glycogen depletion varied; such that at time 0, muscle slices from some fish had glycogen levels of 5–7 μmol/g, whereas those from other fish were 1–4 μmol/g. The variation in postexercise glycogen levels among the fish may reflect differences in how well they exercised. On the basis of observations during the swimming period, some fish simply did not swim as long or with as much vigor as did others. Nonetheless, it became immediately obvious from the data that the responses of muscle glycogen metabolism to the various treatments were dependent on the initial (i.e., time 0) glycogen level (Figs. 5, 6, and 7). In all muscle slices from exercised fish, in the absence of any hormonal stimulation, there was net glycogen synthesis, a reduction in relative GPase activity (Figs. 5B and 6B), and increase in relative GSase activity (Figs. 5C and 6C). However, the total amount of glycogen synthesized and the relative stimulation of GSase activity over the 60-min period was greater in the fish where time 0 glycogen was <5 μmol/g compared with muscle slices with >5 μmol/g (Fig. 5A vs. Fig. 6A and Fig. 7, A vs. C). Muscle slices with low (<5 μmol/g) time 0, glycogen synthesized, on average, 4.3 μmol/g of glycogen compared with an average synthesis of 1.7 μmol/g in fish with postexercise glycogen >5 μmol/g (high glycogen). This was also reflected in the relative stimulation of GSase activity. In the low-glycogen muscle slices, fractional GSase activity increased to ∼50% compared with ∼35% in the high-glycogen muscle slices (Fig. 5C vs. Fig. 6C; Fig. 7, A vs. C).
Muscle slices with postexercise glycogen levels greater than 5 μmol/g responded to hormonal stimulation in much the same manner as did slices from resting fish (Fig. 5A vs. Fig. 4A). Dexamethasone, cortisol, epinephrine, and epinephrine plus dexamethasone in combination all stimulated net glycogenolysis and the relative activity of GPase (Fig. 5B and Fig. 7D) and inhibited GSase activity, relative to the control (no hormone) slices (Fig. 5C). However, when glycogen levels were <5 μmol/g at time 0, glycogenesis and the relative activity of GSase were stimulated in all slices (Fig. 6 and Fig. 7A). In the low-glycogen slices, hormonally stimulated glycogenesis was about 2.5 times greater than in the absence of hormonal stimulation, and GSase activity increased by about another 15% (Fig. 7A). The total activities of GSase (1.13 ± 0.08 U/g; n = 23) and GPase (0.74 ± 0.06 U/g; n = 23) were not different in any treatment.
The influence of time 0 (or initial) glycogen content on the response of muscle GSase and GPase to hormonal stimulation is best illustrated in Fig. 7, in which the relative GSase (Fig. 7, A and C) and GPase (Fig. 7, B and D) activities are plotted against the corresponding initial glycogen levels for each individual tissue slice. The relationship between relative GSase (Fig. 7, A and C) or GPase activities (Fig. 7, B and D) and initial glycogen content, as defined solely by time 0 and the control values (no hormone treatment; dashed lines) did not differ between those slices with low or high glycogen content. However, when the treatment groups are included (Fig. 7, solid lines), the relationship between GSase and glycogen is not altered in the low-glycogen (<5 μmol/g) group (Fig. 7A), rather it is simply extended. In contrast, in the tissue slices with time 0 glycogen levels >5 μmol/g, the relationship changes significantly (slopes of the two lines are significantly different, P < 0.01). Similarly, when defined solely by the time 0 and control data, the effect of glycogen levels on the relative activity of GPase was independent of initial glycogen content. The lines defining this relationship are not different between slices with low and high glycogen content (Fig. 7, B and D; dashed lines). Inclusion of the treatment groups (Fig. 7, B and D; solid lines) altered the relationship between GPase activity and glycogen content compared with that defined by the time 0 and controls (Fig. 7, B and D; dashed lines significantly different from solid lines) but was independent of initial glycogen content (Fig. 7, B and D; solid lines are not different).
Preparation Viability
The muscle slice preparation is a viable and useful system in which to study the regulation of glycogen metabolism in trout muscle. Evidence of viability includes the observations that slices from resting fish maintained oxygen consumption at a steady rate and slices from exercised fish showed an enhanced oxygen consumption, which probably reflects the considerable synthetic activity occurring in these slices. Exercise reduced ATP, PCr, and glycogen levels and elevated lactate levels in muscle, and within 60 min of incubation, all had returned to levels typical of a resting fish (e.g., Fig. 2, B vs. A). Furthermore, and most importantly from the point of view from this study, muscle slices were responsive to hormonal stimulation, as indicated by the increase in glycogenesis in response to glucagon (Fig. 3A).
Resynthesis of Muscle Glycogen After Exercise
Glycogen-depleting contractile activity, in the absence of any hormones, stimulated glycogen synthase activity and net glycogenesis, with the amount of glycogen synthesized and the relative increase in glycogen synthase activity dependent on the glycogen levels at time 0 (Fig. 7, A and C). The variation in postexercise muscle glycogen levels most likely reflects a difference in the amount depleted, because it was observed during the exercise protocol that some fish did not exercise to the same extent as others. Such individual variation in swimming performance is not unusual in domestic rainbow trout (e.g., 25). Muscle slices with an average postexercise glycogen level of 2.8 μmol/g synthesized, on average, 3.4 μmol/g of glycogen (Figs. 3 and 6A) compared with only 1.3 μmol/g synthesized when postexercise glycogen was higher, 6.5 μmol/g (Fig. 5A). The relative activity of GSase I was significantly correlated with initial glycogen content; the lower the postexercise glycogen, the greater the relative activity of GSase, the greater the amount of glycogen synthesized (Fig. 7, A and C). The relative activity of GPase in the absence of hormonal stimulation was significantly correlated with glycogen content, but the relationship between GPase activity and glycogen levels was independent of initial glycogen content. These data suggest that in trout muscle, endogenous glycogen regulates its own synthesis via alterations in the relative activities of GSase, such that at lower glycogen contents, there is greater synthesis.
Glycogen autoregulation, the ability of glycogen to regulate it own synthesis, has been previously observed in human and rat muscle (5, 21, 33, 36) and is thought to be due to a combination of glycogen feedback inhibition on glucose transport, hexokinase activity, and GSase activity (e.g., 15, 27, 41). Glucose transport and hexokinase activities are probably not important regulatory sites for glycogen synthesis in trout white muscle because, even though muscle does have glucose transporters, (9, 28, 48), glucose is only poorly utilized by muscle (e.g., 39, 40, 51) and hexokinase activity is extremely low (26, 35, 47). Rather, the major regulatory site for trout white muscle glycogenesis is most likely glycogen synthase.
There are several factors that can regulate GSase activity, including covalent modification (via phosphorylation-dephosphorylation) and allosteric regulation (e.g., changes in glucose-6-phoshpate, Pi, ATP, and AMP; for reviews, see Refs. 1, 11, 23). Allosteric effects on GSase activity could not be detected by the assay methods used in the present study, so only covalent modifications (i.e., phosphorylation-dephosphorylation) could cause the observed changes in GSase activity. How the actual phosphorylation state of GSase is regulated in fish muscle remains to be determined.
In mammalian skeletal muscle, the relative activity of GSase is a function of its phosphorylation state; with dephosphorylation activating GSase, thus promoting glycogen synthesis. Mammalian muscle GSase has multiple phosphorylation sites, which are dephosphorylated in a hierarchical manner (1, 43), setting the stage for differential regulation of GSase activity. In mammalian skeletal muscle, glucose-6-phosphate can increase the relative activity of GSase by binding to it and exposing phosphorylated sites to protein phosphatase action (49). However, the influence of glucose-6-phosphate on GSase activity varies depending on the extent of glycogen depletion. For example, in glycogen-depleted human muscle cells in culture, the initial insulin-independent phase of glycogenesis is a function of the amount of glycogen depletion rather than the level of glucose-6-phosphate (33). In muscle cells overexpressing GPase activity, glycogen depletion was greater than in controls when incubated in glucose-free media and showed greater stimulation of GSase activity despite similar glucose-6-phosphate levels (33). Thus the argument has been made that the rapid, insulin-independent phase of glycogenesis commonly seen in mammalian skeletal muscle is due to the stimulating effect of glycogen-depletion on GSase activity (6, 33). A similar mechanism may be operating in glycogen-depleted trout muscle, because in vivo there is a significant increase in GSase activity in the absence of changes in glucose-6-phosphate levels (31). It has been suggested that because GSase and GSase phosphatase are bound together in a glycogen-glycogenin protein complex, depletion of glycogen may increase exposure of the phosphorylated site of GSase to phosphatase activity, an effect that may be enhanced by glucose-6-phosphate (e.g., 23). Certainly the data from the present study (Fig. 7) are consistent with the notion that glycogen depletion per se is a driving force for glycogenesis, because the greater the exercise-induced glycogen depletion, the greater the relative activity of GSase and amount of glycogen synthesized.
Hormonal Regulation of Muscle Glycogen Resynthesis
Both glycogenesis and glycogenolysis were responsive to hormonal stimulation. In slices from resting fish, glucagon stimulated net glycogenolysis (Fig. 3A) but did not further stimulate glycogenolysis in slices from exercised fish with already depressed glycogen levels (Fig. 3B). Rather, in slices from exercised fish, glucagon inhibited net glycogenesis (compare glucagon treated with control, Fig. 3B). This response of muscle to glucagon is consistent with what is known about the physiological role of glucagon in fish, which is primarily that of a hyperglycemic hormone (34). This inhibition of net glycogenesis by glucagon in muscle from exercised fish may reflect glycogen cycling. Whereas GSase activity may increase because of glycogen depletion (see Resynthesis of Muscle Glycogen After Exercise), the presence of glucagon would continue the activation of GPase, thus a situation may arise in which any glycogen synthesized is rapidly broken down. Evidence for such glycogen cycling in fish muscle comes from the study of Pagnotta and Milligan (39), in which muscle incorporated radiolabeled carbon from the blood into glycogen in the absence of any net synthesis.
The most interesting and novel finding of this study is the "dual" effect of epinephrine and cortisol on muscle glycogen metabolism. The response of muscle glycogen metabolism to epinephrine, cortisol, and its analog dexamethasone-21-phosphate was dependent on initial glycogen levels (Figs. 4–7). In muscle slices from resting fish in which glycogen levels are "high," the predominant response to epinephrine, cortisol, and dexamethasone-21-phosphate was stimulation of GPase a activity and net glycogenolysis. This is the expected response of muscle to at least epinephrine, which probably stimulates glycogenolysis via β-adrenergic activation of the cAMP-signaling pathway (29). The mechanism underlying the stimulatory effect of cortisol on muscle glycogenolysis is less well understood but could be due to a direct effect of cortisol on GPase a activity, because there was no effect on total GPase activity. Cortisol has been shown to activate GPase a activity in rat hepatocytes in the absence of protein synthesis or any change in total GPase activity, arguing for a direct, nongenomic effect (3, 18). A similar mode of action has been proposed for the action of cortisol on trout muscle in vivo (31).
It was in muscle slices from exercised fish, however, that the dependence of the effects of epinephrine and cortisol on initial glycogen content became apparent (Fig. 7). From inspection of the data (Fig. 7), it was evident that a postexercise glycogen level of about 5 μmol/g was the response-determining threshold, although it is not at all clear what determines this threshold. In mammalian muscle, Price et al. (41) have also suggested that a glycogen concentration threshold is required for the initial rapid glycogen resynthesis seen after glycogen-depleting exercise.
Trout muscle slices from exercised fish with postexercise glycogen levels <5 μmol/g consistently synthesized anywhere from 8.1 to 9.8 5 μmol/g of glycogen in response to epinephrine, cortisol, and dexamethasone-21-phosphate treatment, compared with a net glycogen synthesis of ∼4.3 μmol/g in the absence of hormones (Fig. 7). This stimulation of glycogenesis was coincident with a greater stimulation of GSase I activity in the treated (∼58–63% of total activity as GSase I; Fig. 7A) compared with control (∼46% of total activity as GSase I; Fig. 7A) slices, without any changes in total GSase activity. The relative activity of GPase was unaffected by hormone treatment in these low muscle glycogen slices (Fig. 7B). When postexercise glycogen levels were >5 μmol/g, in the absence of hormones, there was a low level of glycogenesis (1.7 μmol/g). In the presence of epinephrine, cortisol, and dexamethasone-21-phosphate, however, glycogenesis was inhibited (Fig. 7C). Instead GPase a activity was stimulated over that of GSase I (Fig. 7, C and D) and glycogenolysis dominated.
These data suggest that in trout muscle endogenous glycogen not only regulates it own synthesis, but it can also override other stimuli and influence the direction of glycogen metabolism. This is perhaps best illustrated by the relationships between the relative activities of GSase and glycogen content (Fig. 7, A and C). In the absence of any hormonal treatment, the relationship between GSase and glycogen content in control (i.e., untreated) muscle slices with high and low initial glycogen content is not different. This suggests that the controlling features are similar in the two groups and explained primarily by initial glycogen content as those with higher initial glycogen content experienced less activation of GSase and less net glycogen synthesis. However, with hormone treatment, the relationship in those slices with low initial glycogen did not change compared with that defined by untreated and time 0 alone; rather the line was simply extended, suggesting that glycogen content is likely a key regulatory agent (Fig. 7A). In contrast, the nature of the relationship changed significantly with the inclusion of hormone-treated data in the slices with high initial glycogen content, suggesting that hormonal signaling dominated (Fig. 7C).
The surprising observation was the stimulatory effect of epinephrine and cortisol on glycogenesis and GSase activity in muscle slices from exercised fish with initial glycogen levels less than about 5 μmol glucosyl units/g (Fig. 7, A and B). Low glycogen levels themselves can stimulate glycogenesis by enhancing the exposure of GSase to phosphatase activity, and the lower the glycogen level, the greater the stimulatory effect (8). How cortisol and epinephrine enhance this glycogen-depleting effect observed in this study is unknown. There is only one other study that we know of which shows that epinephrine can stimulate muscle glycogenesis. In both red and white muscle isolated from exercised lizards (Dipsosaurus dorsalis; 17), epinephrine stimulated lactate, but not glucose, incorporation into the muscle glycogen pool. The physiological significance of this response may be to accelerate lactate clearance postexercise when both blood lactate and epinephrine levels are elevated, although the underlying mechanism is not at all clear. Epinephrine usually stimulates net glycogenolysis in skeletal muscle and would be expected to inhibit net glycogenesis. This effect is in fact observed in insulin-stimulated glycogenesis in rat skeletal muscle, but surprisingly, epinephrine did not inhibit glycogenesis stimulated by glycogen-depleting contractile activity (16). As might be expected from the effects on net glycogenesis, epinephrine decreased the relative activity of GSase I in insulin-stimulated muscles but increased it in contracted (or exercised) muscles. It was reported (12) some 40 years ago that muscle contraction can reduce the inhibitory effect of epinephrine on glycogen synthase activity, although the mechanism is still unresolved. Given that the relative activity of GSase was greater in those muscle slices with lowest initial glycogen levels (Fig. 7A), there was likely a difference in the degree of dephosphorylation at low vs. higher glycogen levels. This differential effect of epinephrine on glycogen metabolism at high and low glycogen content may be influenced by the fact that more (and therefore different) sites of GSase are dephosphorylated, though the nature of the influence is not clear. Nonetheless, these data are consistent with the suggestion that glycogen depletion is a major driving force for glycogenesis.
The results of this study clearly demonstrate that muscle is capable of in situ glycogenesis and that the amount of glycogen synthesized is inversely correlated to initial glycogen content and can be modified by epinephrine and cortisol. Glycogenesis in trout muscle in vitro is not a novel observation, because Wang et al. (50) reported significant postexercise glycogen synthesis in a perfused trunk preparation within the first 30 min after exercise. Whereas the absolute amount of glycogen synthesized was greater in the perfused trunk (∼30.5 μmol glucose units/g in 30 min) than in the muscle slices (2–4 μmol glucose units/g in 60 min), glycogenesis was greater in both preparations than in trout muscle in vivo (<0.5 μmol glucose units/g in 60 min; 13, 38). Typically, in vivo, there is no net glycogenesis within the first hour postexercise (30), whereas in vitro, glycogen levels returned to levels typical of resting fish within the hour if postexercise glycogen levels were "low." The differences between in vitro and in vivo glycogenic rates cannot be attributed to differences in postexercise glycogen content alone, because at similarly low postexercise glycogen levels (<5 μmol glucose units/g) there is no net glycogenesis in vivo (30). It has been proposed that in vivo, exercise-induced elevations in plasma cortisol may be inhibitory to muscle glycogenesis (13, 38). In the perfused trout trunk, it was suggested that it is because of the absence of cortisol (and perhaps other hormonal stimuli) that glycogenesis proceeds at a greater rate in vitro than in vivo (51). It is here, however, where inconsistencies between the data of the present study and in vivo observations emerge. In vitro, cortisol elevation stimulates glycogenesis when postexercise glycogen levels were low (<5 μmol glucose units/g), and it stimulates glycogenolysis at higher glycogen levels. In the presence of cortisol in vivo, even when postexercise glycogen levels are <5 μmol glucose units/g, there is no net glycogen synthesis (13, 38). It appears that in vivo, cortisol inhibits glycogenesis, an effect that does not appear to be overridden by the extent of glycogen depletion. This apparent conflict in the role of cortisol in vivo compared with in vitro indicates that regulation of glycogen metabolism is, indeed, complex. Nonetheless, these data indicate that epinephrine and cortisol directly affect muscle glycogen metabolism and that, depending on the extent of glycogen depletion, the nature of the response varies.
A Discovery Grant to C. L. Milligan from the Natural Sciences and Engineering Research Council of Canada provided financial support for the research presented in this paper.
We thank C. Johnston for excellent technical assistance and the labs of Drs. D. G. McDonald and C. M. Wood for assistance with the enzyme assays.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2004 the American Physiological Society


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The inaugural Oscar Peterson International Jazz Festival is set to take place in the picturesque Niagara region during the weekend of February 16-18, 2018. As the world's first (and only!) jazz festival bearing the name of Canada's greatest jazz artist, it will honour and celebrate his legacy of excellence. The Festival is led by Artistic Producer Kelly Peterson, Artistic Director Renee Rosnes, Executive Producer Bravo Niagara! Festival of the Arts, and Public Relations & Social Media Director Céline Peterson. This festival is not intended to simply present concerts in tribute to Oscar Peterson, but rather to truly present jazz, showcasing the rich history and the future of the music Oscar loved.
The Oscar Peterson International Jazz Festival gratefully acknowledges the financial support of Presenting Sponsor TD Bank, the Government of Canada, and the Government of Ontario. This event has also been financially assisted by the Ontario Cultural Attractions Fund, a program of the Government of Ontario through the Ministry of Tourism, Culture and Sport, administered by the Ontario Cultural Attractions Fund Corporation.
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Premises liability law is the body of law which makes the person who is in possession of land or premises responsible for certain injuries suffered by persons who are present on the premises. Cases involving people who have suffered an injury may include slip and fall accidents, injuries from inadequate maintenance, dangerous conditions that are not repaired or warned of, or injuries from inadequate security on a property.
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I was just going to ignore this, but…
As you probably know, Pat Roberston called for the assassination of Hugo Chavez, the semi-democratically elected leader of Venezuala, and he is getting pilloried by both the right and the left on this…
Many are calling his remarks 'un-Christian.' I don't know that that criticism is appropriate. Certainly it is not clear to me that assassination would be precluded by the Christian faith.
This was my response:
That is a really scary comment. What is more scary, is that it can be true – depending on your interpretation of the Bible. If you follow J.C.'s version of Christianity – then no. Killing is simply not condoned at all.
If you read the Old Testament – then heck yeah – kill all of the folks that you do not like, that live in your land, that look askance at your goats. This is what Abortion bombers and radical Irish Christians use to justify their deadly practices.
I applaud the Christians that have actually come out and said that Robertsons comment was way out of line. To have a Religious leader from the US talking about an act of Terror like assassinating someone? That is what we are supposedly fighting against.
Now, I do not advocate any legal action against Mr Robertson. He is well within his 1st Amendment rights to say such a thing. However, I feel that it is on our shoulders as fellow Americans to show the world that we do not agree with an extremist view such as that.
If the people of Venezuela disagree with what their leader is espousing, then it is their job to make this thing right. America does not have the resources, nor the right to topple every government that we disagree with, and replace it with one of our liking.
I do feel that democracy is probably one of the better forms of government that is out there, however it is not our place to force the rest of the world to follow our lead. When they are ready, They will do it. We have seen it in the former USSR and other places. People are generally smart creatures. They can usually see what is up, and make choices to take action.
Dave went further in a comment:
Particularly that no dictorship has a right to exist, and any free nation wishing to topple a dictorship has the moral right to do so. Protecting another people from aggression, even agression from their own government, is allowable under just war theory.
I follow the 'Do not start it, but finish it' theory. There is no such thing as a 'just' aggressive act. The only time that violence can be justified in any way is in self defense. That includes Governments – the only time that we can justify war is when we are attacked.
The 'war on terror' is justifiable. WW II was justified. Iraq was not. Toppling Governments because we disagree with them is certainly not justifiable.
However, since we are in Iraq, we need to make sure that the job is 100 percent done before we leave. If we just leave now, the Iraqi people will be left in quite a lurch. No stable government, no stable anything. Many would die, and more then likely, the country would be worse off then when we started over there.
That is unacceptable. We created this mess – we need to fix it. I fully support the troops, and Bush in saying that we have to stay the course.


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For all those that have yet to fall off the New Year, New You wagon get your veggie fix Scandi style with KuPP's delicious Veggie Bord. Packed full of nutritious ingredients, the Veggie Bord is piled high into a marvellous medley of: quinoa, kale and butternut bites; earthy roasted beetroot and smoked red onions; Swedish Västerbotten and Danish blue cheeses as well as delightful dips to dunk hunks of sourdough and crispbread into.
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Imagine… taking the hard work out of writing effective copy for your website, your social media posts, blogs and LinkedIn.
Imagine… customers telling you that finally they feel like they have been heard, that they feel understood and how do they pay you – NOW!?
Finally, you will begin writing copy that WORKS!
Persuasive, effective copy has the power to transform your business.
Join Paul Counsel and Nicole Ashby for The Science, Art And Psychology Of Persuasive Copywriting Strategies Used By The World's Greatest Influencers To Produce Higher Conversions, More Buyers and Fewer Rejections.
Please note this is not an introductory session. You must already be actively writing for your business, whether it be social media posts, media articles, blogs or copy for your website.
Date and Time
Bendat Community Centre, Telethon Speech & Hearing
Dodd St
Wembley, WA 6014
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This property has been removed by the agent. It may be let or temporarily removed from the market
Property Description
Letting information:
|Added on Rightmove:
|02 November 2016 (322 days ago)
Key features
- Sought after location
- Long Term
Full description
* Bulmer Estates are pleased to offer this NEWLY decorated mid terrace house.* Smartly presented throughout, with BRAND NEW carpets. * Fitted kitchen with integrated oven/hob. Spacious lounge. Downstairs wc. * Two double bedrooms. Jack and Jill bathroom, white suite and shower over bath.* Enclosed private rear garden with patio area and shed. Off street parking. Band B.
* In addition to the rent and deposit, application fees are payable - £199 for up to two adults and £50 for each additional adult* NO FEES are charged for guarantor applications, tenancy renewals, house visits, check outs or for supplying references for outgoing tenants.* Please download our `How to apply` form for full terms and conditions from our website www.bulmerestates.co.uk or phone us on 0115 969 6111.
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