text:
Do you have a question idea? Submit yours here.
- Macy's — 15% off beauty purchase with code VIP, ends 9/25.
- Neiman Marcus — Receive up to a $300 gift card with $250+ purchase with code SEPTGC (beauty/fragrance included!), ends 9/20.
- Saks — Earn a Gift Card up to $700* with code SEPT2017, ends 9/21.
Do you have a question idea? Submit yours here.
51 Comments
Discussion and debate are highly encouraged, and we expect community members to participate respectfully. When asking a question, please check the post above for information regarding pricing, availability, dupes, and availability, and keep discussion on-topic. If you have general feedback, an off-topic question, or need technical support, please contact us.
Comments that include advertisements, self-promotion, insults, etc. may be in violation of our comment policy and subject to deletion. Please see our comment policy for more information.
Comments on this post are closed.
I'm not as good as I should be, I can think of at least three times in my life when I fell asleep without removing makeup.
I take my makeup off 99% of the time… I think there have been three times I've gone to bed with it on in the last few years, and all involved heavy amounts of alcohol and exhaustion! I don't always go through a full cleansing routine but at the very least I use makeup remover on some cotton pads.
Always!! I can count on one hand the times I haven't since I was 12 years old. It's my favorite part of the day.
I am HORRIBLE at taking off my makeup. Sooooo bad. Making sure I remove it every night is one of my current short term goals.
I think I'm alot better at removing my makeup than I used to be..
The fact that if I don't, my skin really does suffer.. and so that's what makes me keep up my routine
I'm very thorough, even if I happen to fall into bed at 3 or 4 am. I just can't stand the feeling of makeup that has been sitting on my face all day and all night long, it makes me feel grimy. I also happen to own almost only white and ivory sheets and bedclothes, and I know that taking off my makeup at night is still less works than having to wash them in the morning.
I just wrote a blog post about this! I am really good most of the time but sometimes when I've been out drinking and I get home all I want to do is eat a kebab and roll into bed…
Absolutely terrible! I go to bed without taking off my makeup off far too often.
Worse than I should be. Two to three times a week I do before bed. I do clean, tone and moisturize every day, and I get complements on my skin in the store when I shop for products. No one ever tries to sell me on skin care, because I am lucky enough not to have issues, even though I regularly fall asleep with makeup on.
Always!! Most days I wash my face the instant I get home from work.
Always, without fail, no matter how drunk I am. Can't STAND the feeling of it, so despite the fact I've worn it everyday for the last 7 years, still haven't gotten used to it.
I am TERRIBLE about it, especially now that I'm pregnant and have a bad habit of falling asleep on the couch every night around 8:30. I've gotten bad about taking my contacts out, too. I need to work on just taking my makeup off, moisturizing, and putting my glasses on as soon as I get home from work!
Hi Christine!
I am pretty good about it! There's maybe a night or two that I can recall where I didn't take any makeup off (*ducks*), but those are usually days I wear none or minimal makeup. I usually at least use a cleansing wipe. I am to do my full skincare routine with wash, tone, treatment, moisturizer. I do that the majority of the time 🙂 I will say I've consistently followed a morning and night skincare routine for about 10 years now, since I was a teenager!
I'd say very good as well. I can only recall 2 times I've ever fallen asleep with my makeup on (not on purpose!). I try to keep makeup wipes nearby to avoid that happening. At the very least, I'll use a makeup wipe to remove makeup when I'm super tired. For the most part, I use cleansing oil and warm water at the end of the night.
I'd say I'm quite good, but there were occasions where I'd fall asleep unintentionally. Like, curl up with a magazine or a book on the sofa to unwind for half an hour — come to senses five hours later closer to waking time than bed time.
Oh my…I religiously remove my makeup at night! In my 20's I was not as good about this but now that i NEED skincare products at night I absolutely remove makeup and apply skincare products.
I have never gone to bed with makeup on.
I /always/ take off all my makeup I get home. For some reason the minute I step into my house it feels like I'm wearing a mask and it's very uncomfortable. I'm not wearing a ton of makeup either. It won't feel like that when I put it on or at any point when I'm out. I guess it's like wanting to rip off the bra when you set foot in your door lol.
I'm always sure to remove my makeup because back in high school, I had a classmate who slept in her makeup and woke up with a nasty eye infection that had her wearing an eye patch to school for days. It was the juiciest gossip at the time, quite sadly. I've no desire to end up with an eye infection like that!
I've become very ritualistic at night before bed and I can't stand the feel of makeup on my face after a certain period of time. If I'm really feeling overly tired or sick, I will still grab one of those quickie towelette thingees to take the worst of it off at a minimum. But that is rarely enough for me either. If I do not do my full skincare regimen, I can really see the difference the next morning. You get to 50 and you will understand. You wake up with skin creases that seem to stick! So not worth skipping that extra 5 minutes.
99.9% of the time my makeup gets removed. The remaining 0.01% is usually a result of excessive alcohol and staying up REALLY late – I have done it once that I can think of this past year and that was at my best friends birthday party, I woke up at dawn with my beautiful green glittery eye makeup completely intact (impressive, but beside the point!).
I nap during the day about once a week and I never take my makeup off for that… but if it isn't at the end of the day, does it even count? 😉
I am so compulsive, I have never missed a day. I use Lumene oil to dissolve and remove my eye makeup and then use baby shampoo to wash my face. Then I put on a moisturizer and an oil over that and that's my nightly routine every night.
I have to no matter how tired I am. It's not even a choice. Otherwise I'll breakout the next day.
Very good, every night! Cant stand the feeling of makeup left on your skin when you wake up, and its also terrible for your skin. I'm 26, and glad I got in the habit of being good about it now cause I know a lot of people older who never did and that regret it. I'm the same, will slip up the odd time but very rare!
I think I'm very good about it now. In the past when I didn't remove my eye makeup, I would get styes. I hate those little buggers, so I started getting really diligent about cleaning it all off. I also have always had bad skin, so I figured it's the least I can do. Now on the days I don't wear makeup, it's a different story – I don't always wash my face…but I love my skincare regime (Paula's Choice), so I'm getting much better!
I remove all my makeup before bed. It's a total must.
I am all about skincare, and I have sensitive skin, so sleeping in makeup is not an option at all for me. I typically opt to remove my make up once I'm in for the day. If I don't remove it when arriving home, I do so during my nightly shower. On the rare occasion that I arrive home at a late hour and I'm exhausted, I just use a make up remover wipe and call it a day.
I am STRICT about it! Even if it's 3am and alcohol flushed. Not that I can't stand my face with with makeup on when I sleep, it's just that my PILLOW IS SACRED and who wouldn't want to sleep with a freshly washed face?
I used to sleep in my makeup all the time until I realized that was one of the most damaging things to skin. Now I'm so used to taking it off that it feels pretty gross when I have forgotten so I'll do it even if I'm about to drop. I just think of my dirty face, the dirty pillow and the break out that follows.
I always wash off my make-up because I don't like how crud gets in my eyes and makes them uncomfortable. If it weren't for that, I probably would get lazy….
I just try to tell myself that it is helping me relax for bed.
I always remove my makeup. First I use a removing wipe, then a cleansing water. After that I wash my face and it's ready for my night cream!
Im terrible!!!!
I always remove my face makeup, but I sometimes leave my eye makeup on and use it as a smudged smokey look the next day.
If you don't see your comment yet, it's still in our moderation queue. We try our best to approve comments within 24 hours (and reply to them within 72 hours) but can sometimes get behind. Thank you for your patience!


text:
Browse Directory
Airlines (57)
Destination Guides (55)
Hospitality (7)
Hotels and Accomodation (369)
Online Reservations (176)
Preparation (7)
Property Rentals (22)
Specialty Travel (34)
Transportation (113)
Travel Agents (133)
Vacation Packages (109)
Links Sort by: PageRank | Hits | Alphabetical
|Mauritius Honeymoon Packages - http://www.mauritiushoneymoonpackages.org/
Mauritius Honeymoon Package a perfect way to start the journey of a married life. Mauritius Honeymoon Packages offer Honeymoon Packages in Mauritius, Honeymoon Tours to Mauritius, Mauritius Honeymoon, Honeymoon in Mauritius, Honeymoon Package Mauritius, Mauritius Honeymoon Tour Travel Packages.
|Mauritius Tour Packages, Mauritius Package Tours - http://www.mauritiustourpackage.com/
Mauritius Tours is renowned for its water skiing, scuba diving, sailing and for its beaches. We offers Tour Packages to Mauritius to enjoy its heavenly ambiance, cool amiable climate, fascinating charm and marvelous attractions have made it a honeymooner's paradise.
|Flights to Ghana - http://www.flightstoghana.co.uk/
Plan your holidays to Ghana and book cheap flights with us. traveling with a reliable operator with years of experience in getting UK travelers flying to Ghana. There's exotic scenery, unique cultures and a wide variety of game parks, natural highlights. Also get Direct and indirect flights to from Accra to UK.
|Flights to Lagos - http://www.flightstolagos.net/
Find discount airline flights to Lagos from UK. Flights to Lagos has unlimited discounted flights. Call us on 0207 871 4545 and get the cheapest available flights.
|Andaman Nicobar Tours - http://www.andamanandnicobartour.com/
andaman Nicobar Tours offer natural beauty, azure sea with gleaming sandy beaches dotted with palm trees, rich flora and fauna, wildlife parks & sanctuaries, coral reefs, rich marine life and scores of fascinating tourist attractions.
|Abuja Flights from UK - http://www.flightstoabuja.com/
Abuja is the capital city of Nigeria. Here you can get cheap air tickets. Feel free for contact us dial 0207 871 4545.Online Flights booking provide you fully cheap rates.
|Cheap Flights to Lagos from Uk - http://www.cheapflights2lagos.com/
Fly to destinations across the World with Us. Find a great deal on cheap flights to Lagos with our Experts dealing with travel and holidays worldwide. Flights to lagos understand that our customer enjoy the feelings of a home away from home. Compare the best cheap holiday deals and cheap holiday offers.
|Taxis in Oakleigh Park - http://oakleighparkcarsminicabs.co.uk/
Call now 0208 449 0000 London Heathrow fr £35,Gatwick fr £65,LUTON fr £32,Stansted fr £38,kings cross/ St Pancras,£25.00,Euston £25.00
|:San Carlos Taxi Cabs & Limousines - http://www.sancarlostaxicab.com/
San Carlos Taxi Cabs & Limousines provide taxicabs and Limousine services for dedicated use to business executives and travelers in the California Bay Area. We offer the highest quality transportation services, at reasonable rates, without sacrificing your comfort or safety. Our customers can depend on us to provide them excellence in customer service while maintaining high quality and safety standards. Taxi cab Limousines San Carlos also provides Napa Valley wine tour by limousine, San Francisco city tours and other Northern California limo tours. Charter Limousine service services such as wedding, bachelor/ette parties, prom packages and nights on the town in San Francisco are also available! San Carlos Taxi Cabs & Limousines
|Niagara Fall Tours Taxi - http://www.jetlimousine.ca
Jet Limousine serve as most affordable taxi service at Burlington airport, Milton airport, Oakville airport, Hamilton airport and Niagara falls taxi service
|Solimar Holiday Villas - http://www.solimarvillas.com
Solimar Villas - Holiday Villas and Apartments on the Costa Blanca. We are registered with the Valencian Tourist Board so you can choose one of our holiday properties with confidence.
|Kyrenia - http://www.directtraveller.com/
When you arrive in Kyrenia North Cyprus the amazing views of the Five Finger Mountain range will appear from every location in Kyrenia.
|Taxi in Hadley wood - http://hadleywoodcarsminicabs.co.uk/
Call now 0208 449 0000 London Heathrow fr £35,Gatwick fr £70,LUTON fr £32,Stansted fr £38,kings cross/ St Pancras,£26.00,Euston £26.00
|Airport Transfers | Airport Bus - http://www.eairporttransfers.com.au/
A website dedicated to airport transfers and airport bus transfers.
|Cabs in Woodside Park - http://woodsideparkcarsminicabs.co.uk/
Call now 0208 449 0000 London Heathrow fr £33,Gatwick fr £65,LUTON fr £34,Stansted fr £40,kings cross/ St Pancras £20.00,Euston £20.00
|Tour Mongolia - http://www.selenatravel.com
Selena Travel is the best tourism company offering very affordable Mongolia Tours and Travel services. Contact www.selenatravel.com and make your travel to Mongolia more enjoyable and memorable.
|Egypt Tours - http://www.travelband.net
We Offer a range of Egypt tours and Egypt Travel Packages, Egypt Nile cruises. Travel to Egypt and Jordan, Egypt excursions and Egypt Safari.
|Philippine Culture, Nature and Leisure - http://www.banaue-tours.com
Presents the best tours for mountain province area. We are proud to say that we have years of experience with tours all over the Philippines, and specializing in Northern Luzon, Where banaue rice terraces can be found
|London party boats - http://www.kingcruises.com
King Cruises Thames Boat Hire are Londons leading company for Wedding boat parties, Wedding boat parties London, Corporate boat parties, Corporate boat parties London, Thames party boats, party boat, Thames party boat discos, Boat Hire Thames
|Chennai Car Rentals - http://www.chennaicarrentals.in/
Chennai Car Rental - Make a car online reservation at a cheap price from Chennaicarrentals. We are the No.1 car rental agency in Chennai with over several locations and competitive car hire rates.

subset: commoncrawl


text:
I've heard some crazy things over the years and I often wondered what percentage of investment managers and advisors have an element of "astrology" to their decision-making process. Nonetheless, I never actually thought someone would use "relationships(s) between astronomical phenomena and events in the human world" to make investment decisions.
Turns out that Gurudeo Persaud, formerly an employee of Money Concepts Capital Corporation, did use astrology to make investment decisions and the SEC is busting him for it. Well, actually they are busting him for not disclosing that he was using insane concepts to pick stocks–bravo SEC!
The Commission's complaint alleged that, while associated with Money Concepts, Persaud started his own company, White Elephant Trading Company LLC. The complaint further alleged that from no later than July 2007 until at least January 2011, Persaud raised more than $1 million from investors by promising them 6 to 18 percent annual returns and a risk-free investment in White Elephant's private equity fund, which would invest in the futures and other markets. Persaud made numerous misrepresentations and omissions to investors, including guaranteeing their investments were secure, failing to disclose his trading strategies were based on lunar cycles and the gravitational pull between the Earth and the moon, and that he misappropriated nearly half the investors' contributions for personal use.
source: http://www.sec.gov/litigation/admin/2014/34-72145.pdf
What will they think of next?
- The views and opinions expressed herein are those of the author and do not necessarily reflect the views of Alpha Architect, its affiliates or its employees. Our full disclosures are available here. Definitions of common statistics used in our analysis are available here (towards the bottom).
- Join thousands of other readers and subscribe to our blog.
- This site provides NO information on our value ETFs or our momentum ETFs. Please refer to this site.


text:
A good buffet display is an immediate sign of quality and at Adams Catering Hire we can supply you with everything you need to show off your professional buffet. So if you're looking for professional catering or event hire in London then we'll be able to supply you with every buffet essential you could need from cooking implements to tableware and even china.
- Sort by Default Order
- Display 15 Products per page


text:
Cross Promoting Social Media Platforms to Build Your Audience
Everyone wants more followers, and sometimes getting them is easier than you think! Cross-promoting your business's social media accounts is a simple way to boost your fanbase across the board without spending a dime. Here's how to get started: Make cross promotion easy by creating an online document to list your business's social media platforms and a link to each: Create a document in your phone, on your computer, in Google Drive, or wherever else is convenient for you. Having all of your links in one location, and in a social media friendly format, will make promotion on the go incredibly easy. For each social media account: Jot down your number of followers. Copy the link to your public profile. Go to Google's URL shortener Paste your link directly into the box at the top, Click "Shorten URL" Voila! Your link is magically super-short. When you're trying to squeeze your


text:
Price: $97 You must be an active subscriber to view this premium content. Please register
You must be an active subscriber to view this premium content. Please register
Can You Flip A Coin And Pull A Winner At Least ONCE Every Six Tries?
If You Said YES – YOU Just Discovered Ron's Secret To Making Profitable Trades Over 86% Of The Time. … .. … Now THAT's Money Management!
I know – you've probably seen BOATLOADS of robots recently hit the markets and promise the world. . .. ..
LITERALLY every other day lately – I get another request to visit a site with just stupid claims at this point – like 'perfect strategies' and 'precision accuracy'
^ YEAH – RIGHT! Accurately aimed to take your money, and lose it…..Funny thing is – each one I visit, I know it's not going to make my breakfast and fill my mattress with $100's.. .. . But I still buy pretty much all of them – even if it looks fluffy or full of hype (or not enough info). .. ..
Why? To test against our in-house system and see if it outperforms it. Guess what – each time I do this – within a few weeks the 'perfect EA' that promised me bazillions – finally BOMBS


text:
South Central College physical chemistry
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Get info about South Central College physical chemistry, and registering for elective seminars and lectures. Which college degrees give you the best chances of finding employment after graduation? Accredited engineering programs, combined with a brief internship or prior work-experience, offer a great return on your engineering education. Concentrating on South Central College physical chemistry, for example, you may gain an advantage in job recruiting on-campus. Check with the chemical engineering department for course registration deadlines, and organic chemistry lab program requirements. Please use the form above to request admissions info for South Central College.
Chemical engineers apply the principles of chemistry and biology to solve problems in the production of chemicals, fuel, pharmaceuticals, and many other products. They design processes and equipment for large-scale manufacturing, plan and test production methods and byproducts treatment, and direct facility operations. Most chemical engineers have a bachelors degree in chemistry, as well as internship experience. They may spend a large portion of their work day at chemical plants and petrolueum refineries, directing operations or solving chemical reaction problems. The average salary for chemical engineers was $97,360 as reported the US Bureau of Labor Statistics in May 2017.
Biomedical engineers design equipment, devices, computer systems, and software used in healthcare. Bachelors degree programs in bioengineering include extensive organic chemistry lab work, and offer courses such as fluid mechanics, computer programming, chemical reactivity, and biomaterials. Accredited programs also include technical training in engineering design, and practical internships in pharmaceutical manufacturing. The median annual wage for biomedical engineers was $86,220 in May 2017. Further, employment of biochemical engineers is projected to grow 23% in the next decade, much faster than the average for all occupations. Beyond major requirements within the chemical engineering department, you may consider University of Evansville geography courses online as an adjunct to your primary course of study.
Telecom Engineer I - INF001427
XPO Logistics Less-Than-Truckload
Though not required to engineer the product, theumbent should understand the concept of a call center and call flow arrangements needed to run this..
3 days ago STUDENT TRAINEE (INTERDISCIPLINARY ENGINEER)
Department of the Navy
From pipefitters to accountants, scientists to engineers, doctors to nurses-the careers and opportunities to make a difference are endless..
3 days ago Engineer III
3t Systems
Additionally the Systems Engineer III will be responsible for Citrix and/or Microsoft RDS user. The Systems Engineer III will also act as a subject matter..
3 days ago Manufacturing Engineer
Human Resources Professional Search
Hands on manufacturing engineer. Manufacturing assembly processes experience..
4 days ago Mechanical Engineer
Advatech Pacific
Advatech Pacific is seeking a Aerospace or Mechanical Engineer to support upcoming engineering activities at our Tempe location..
23 hours ago Production Flight Line Crew Chief
We are engineers and technicians. Troubleshoots anomalies encountered in aircraft electrical and mechanical subsystems..
1 day ago


text:
In the wake of quarterback JEFF GEORGE's suspension, the Atlanta Falcons re-signed BROWNING NAGLE yesterday. BOBBY HEBERT, who had been playing behind George, will start Sunday when the Falcons face the 49ers in San Francisco.
Nagle, 28, is a five-year veteran who played three seasons with the Jets and one with the Indianapolis Colts before signing with the Falcons last season. He was released in Atlanta's final cut this year. To make room for him, the Falcons released running back LINCOLN COLEMAN.
George was suspended Monday after a sideline confrontation with Coach JUNE JONES during Sunday's 33-18 loss to Philadelphia. (AP)Continue reading the main story


text:
You may have noticed a few changes going around here. I've been fidgeting with the overall look and layout of this blog for awhile, but pre-made WordPress themes allow you to only do so much. I had finally found a theme that I could live with, but I wanted to make sure the header was unique to this blog. Cut to this:
That's right. Soak in the radiance of stick figure art. Needless to say it was a nice surprise when an email from the infamous Dan Solera popped up in my inbox attached with the artwork you see here:
Out of pure generosity and a slow day at work, Dan sketched out the rotting zombies chasing the Lil' Glenn character. Maybe he's just really observant, or maybe I should start to switch up my race day wardrobe, but Dan got all of the details of Lil' Glenn down to the color of shorts/shirt/buff I wear. But he made a few mistakes. He gave me a nice forefoot strike and some biceps. In real life, neither of those things exists. Thanks anyway, D.
So since Mr. Solera decided to do something so nice without an incentive and since we're in the season of giving, I've decided to follow his lead and pay it forward. Leave a comment on this post, and next Friday (December 14th), I'll randomly draw a winner for – wait for it – a FREE PAIR OF BALEGA SOCKS. Everybody loves socks, and this just proves I'll do anything for a comment.


text:
in association with
in association with
he has saved the world more times than you've unclogged your toilet and is possibly the most dangerous man on the planet. but you're safe, providing you don't try and end civilisation any time soon... new Bond for a new era – this is how many see Daniel Craig's 007: a brutal, tough spy that faces real threats and real problems. But he is just the latest in a long line of actors, all of whom have portrayed the most famous secret agent in the world and helped create the second longest-running film series in history (the honor for longest, dubiously, sits with the Carry On series). This creates a long and rich history, so here is our quick guide to the history of the man with a licence to kill...
Words>walt pretorius
Shaken, 034
nov 2008
Nov 2008
in association with
in association with
bond girls
Casino Royale was a remake. Not many people know that, but the original film was made in the late 1950s, and was a comedy. It's best to ignore it, because it was a truly awful film. Instead, a journey through the history of James Bond (in film, at least) should begin where most people think it did. "Bond, James Bond." 1962 first heard those words uttered on the silver screen when a relatively unknown Sean Connery appeared as super-agent James Bond. The film, Dr. No, was a success, and started the longest running series of feature films ever known, on their long journey. But, arguably, it wasn't until 1963's From Russia with Love that Bond's character became solidified. It was this film that defined Connery within the role, taking a deeper look at his background. It also defined a theme that would stay with the films for around twenty years – the Cold War. That said, Goldfinger (1964) didn't have much to do with fighting Communism. Instead, it brought the focus onto extreme capitalism, and pitted Bond against a villain bent on ruining the world economy and enriching himself. 1965's Thunderball similarly ignored the Cold War, and
007 loves the ladies
Honey Ryder
The real question is: considering the amount of cars Bond has destroyed, wouldn't it be cheaper just to let one of the villains win?
Bond is VERY protective of his garden, so leave the plants alone!
introduced another theme that would last through many Bond films – a global organisation, bent on wrong-doing, called SPECTRE. You Only Live Twice, which came out in 1967, threw a little suspicion at the Russians, but it was the same evil group behind the whole plot. This ambitious film is notable for making use (at the time) of the biggest ever sound stage construction. Still the Cold War lurked in the background, but the conflict between East and West would never materialize into anything more than saber-rattling. Connery's Bond was a hero of the time; a man's man, hard drinking, hard fighting and with woman dying to drape themselves over him. His sophistication was a thin veneer over the brutality that he ascribed to, something that wouldn't be seen in another Bond actor until 2006 in Casino Royale. "This never happened to the other fellow" After four films, Connery had had
nov 2008
enough. Worried about being typecast, the now-famous actor decided to try other projects, and the producers of the Bond films were left in a quandary. Who would replace him as the world's greatest secret agent? Their choice was an Australian model named George Lazenby, and he made his first (and only) appearance as Bond in 1969's On Her Majesty's Secret Service. This film is one of the most important in the series, as it deals with Bond's marriage to Teresa, and is formative in his character (because Teresa is killed by SPECTRE.) Yet, despite the importance of this plot-line, the public didn't take well to Lazenby as the super-spy, partially thanks to the producers dubbing a different voice over many of Lazenby's scenes. Lazenby also had notorious difficulties with the film's director, Peter Hunt, so refused to talk to the actor directly. He also was reported as considering the Bond character as out of touch with the liberated Seventies. Although Lazenby was signed for
seven Bond films, he quit – as he was contractually tied to star in Diamonds Are Forever, he received a partial payment for the film anyway, but he refunded this. The producers all but begged Connery to return. He did (though at an astronomical salary, plus the producers had to pay actor John Gavin as well, as he was initially contracted to play the next Bond), appearing for last time officially as James Bond in 1971's Diamonds Are Forever. The film saw Bond's reaction to the death of his wife, perfectly pulled off by the more physical Connery. The wise-cracking, suave Scotsman put the series back on track, but he wasn't going to stay. "Just be disarming, darling" The new Bond was to be an already famous actor. He had been made so by his part in The Saint television series. He brought with him a new suaveness to counterpoint Connery's charming brutality, and a sense of distaste at
violence. He wore double breasted suits and never drank martini, preferring bourbon. His name was Roger Moore. Moore would go on to be the most prolific Bond actor, making more films in the role than any other: seven, beating Connery's six official (not counting one unofficial) cracks at the part. Live and Let Die (1973) was his debut in the role, and he charmed audiences with his upper class manners from the word go. His next attempt at the part came in the part of 1974's Man With The Golden Gun, but it wasn't until the release of The Spy Who Loved Me that he truly became James Bond. Over the next eight years, Moore would reprise the role another four times: Moonraker (1979), For Your Eyes Only (1981), Octopussy (1983) and A View to a Kill (1985). Many Bond fans cite the very science-fiction Moonraker as the worst Bond film ever made, beating even Lazenby's attempt at being poor. During this period, Orion pictures made an unofficial Bond film (the official ones are made by United Artists). It brought Sean Connery back as an ageing Bond in a remake of Thunderball, and was released in 1983. It was called Never Say Never Again. Moore's Bond performances took a swing or two at the still-continuing Cold War, but these adversaries were relegated to the position of "quiet threat," leaving Bond to deal with more
He can fight the world's most evil people, but Bond needs help taking a bath and someone to hold his gun...
(Dr No) Who could forget the Aphroditelike emergence of Honey Ryder from the waves lapping at the shores of Dr No's dangerous island? Her character was very strong for a film of the time, although she had a very poor education. Ryder is easily considered the iconic Bond girl.
Solitaire (Live and Let Die) Kananga's captive tarot card reader, Solitaire was a reluctant Bond girl. Her ability to foretell the future was tied to her virginity (which Bond takes care of in fairly short order) and her habit of attracting voodootypes just caused trouble. She didn't do much to save the day, but for pure beauty few came close.
Pussy Galore (Goldfinger) Although her name was very controversial at the time, Pussy Galore is one of the world's favourite Bond girls. The leader of an elite, all-girl flying team, she was in the employ of the dastardly Auric Goldfinger at the time of meeting Bond. He changed her loyalties in his usual fashion.
Major Anya Amasova (The Spy Who Loved Me) This gorgeous femme fatale sought revenge on the British agent who killed her lover (Bond, of course) but fell for his charms and was instrumental in helping Bond put paid to the plans of Stromberg. She was a Major working for the KGB at the time of their meeting – one of many to fall for Bond's wit and ways.
Nov 2008
in association with
in association with
the bad guys they want it all
Ernst Stavro If there is an iconic Bond villain, it is the head of SPECTRE, Ernst Stavro Blofeld. Blofeld was a foil to Bond in many movies, but his organisation was always bested by the secret agent. His last appearance was in For Your Eyes Only, and ends with Bond dropping the now wheelchair ridden bad-guy down an industrial chimney stack.
immediate dangers. While the Russians were sometimes behind plots, they were never really the ones that Bond pursued. He also brought a new take on the character. He most certainly did not want to emulate Connery in any way, preferring to be a less violent, more sophisticated agent. In fact, the only one true act of willful violence, not committed in self defense, is seen in For Your Eyes Only, when Bond tips a car containing a helpless enemy over the edge of a cliff. By the end of View to a Kill, it was plain that Moore was getting too old for
Auric Goldfinger The German businessman planned to steal all the gold from Fort Knox, crippling the US economy. His henchman, Oddjob, is the famous bladed hat throwing mute Korean. But Goldfinger himself deserves a mention as being one of Bond's toughest adversaries.
Now that we think about it, why doesn't MI6 ever give Bond a 4x4?
Le Chiffre Cornering a vicious creature is a dangerous thing to do, but that's exactly what Bond does to financier Le Chiffre. By sabotaging a number of his business deals and then beating him at a now-famous game of cards, Bond turns Le Chiffre into a desperate man who will stop at nothing. This is one of the few villains Bond doesn't kill himself.
Alec Trevelyan What makes Alec Trevelyan so dangerous is that he once had a double-0 number, just like Bond. His alliance with the remnants of the KGB allows him to take control of a very dangerous satellite. A thrilling fight on a massive dish sees Bond finally beat the man who was once his best friend.
nov 2008
the physically demanding role (he was 58), and a campaign around who the new Bond would be was started as a marketing ploy by the series' producers. Their initial choice was Pierce Brosnan, but his commitment to Remington Steel prevented him from taking the part. And so the new Bond became Timothy Dalton. "I've had a few optional extras installed" Dalton was not happy portraying Bond as a violent womaniser. Well, not as a womaniser at least, and he was responsible for a trend of stronger female characters that have lasted to this day. Although he proved to be a popular choice, Dalton only made two films: The Living Daylights (1987) and License to Kill (1989). The latter was an emotionally charged film which saw Bond go rogue after a drug dealer tried to kill his dear friend, CIA agent Felix Leiter (who had been a character in the films since the Connery days.) But Dalton couldn't handle the pressure that came with the part, and so absconded the throne, making way for the now available Pierce Brosnan.
"James Bond, stiff-ass Brit" The Brosnan era held many highs and lows for Bond, but mainly in terms of the films rather than the persona. Goldeneye, which saw the light in 1995, is counted by many as being one of the best Bonds ever made. The subsequent Tomorrow Never Dies (1997) reached less critical acclaim. Brosnan announced his retirement after the third film, The World is Not Enough (1999) but when it became the highest grossing Bond film ever, he agreed to do one more. 2002's Die Another Day was his final attempt at the role, but the comparatively poor reception the film received ended his Bond career on something of a low note. But Brosnan wasn't all bad as the character, and put in convincing performances for some very difficult times that Bond faced. His incarceration and torture at the beginning of the last film was well handled. However, Bond was no longer faced with SPECTRE, and no longer had to worry about the Soviet Union. The hero was floating in a sea of uncertainty, at least in terms of scripts. Brosnan's Bond was a mix and match of Connery's brutality and Moore's genteel nature, giving the actor a wide appeal.
"Does it look like I give a damn?" Daniel Craig entered the picture with Casino Royale, released in 2006. The film takes the viewer back to the start of Bond's career as 007, creating a chronological anomaly in the series. This, along with the changing face of Bond, hasn't dissuaded either the producers or the fans, though Craig is back in the newly released Quantum of Solace, and has been tipped to play the agent again in what is currently being called Bond 23. According to MGM head Harry Slaon, the actor will appear in two more bonds after that (Bond 24 and 25) but Craig has declined comment. If he does, he will beat Brosnan's four, and be two away from beating Moore's record. The Daniel Craig era, as long as it might last, has brought a more human Bond to the fore. The almost invincible super-agent now has a very identifiable streak. He is weak in some ways, and uncontrolled in others. Gone are the gadgets of yesteryear, too, leaving this new, physically aware version of the character with his wits and his fists to get him through sticky situations. The series shows a new maturity with this fine actor, and a growth that is very obvious – although Craig's nod
to Connery in his portrayal of 007 is undeniable. He is not suave and genteel, but rather brutal and physical, much like the original actor. The scripts are changing, too: the original films were works of fantasy, designed to distract a public wary of an unknown enemy in the East. These days the threats to the world are different, and the popular hero that is James Bond needs to be different too. And Daniel Craig is just the man for the job.
He promised the car they'd go hit the slopes – such a nice guy.
the henchmen
every bad guy needs a right hand (man)
Jaws There is no henchman more iconic to the series than Jaws, the mountain of a man who has a pair of teeth that will bite through anything. He also has the unique honour of appearing in two Bond films and he was never actually killed (but escapes into space with a new girlfriend). He also only said one line throughout all the movies.
Oddjob Jaws may be number one, but Oddjob was the first real henchman. The mute Korean strongman had a knack for throwing his steel-rimmed hat to kill people (and decapitate statues) and he could pack a punch as well. When not killing, he drove Goldfinger's car. Alas, he meets his end with an electrified gate in Ford Knox.
Mr. Kidd and Mr. Wint Often speculated to have been gay lovers, these two assassins were still dangerous and did a lot of killing for Blofeld. They figure out creative ways to kill people (involving scorpions, coffins, etc.) and often had a one-liner to go with it. But they went up in flames on a cruise ship.
Mayday She has the distinction of being the only primary henchwoman in the Bond films (though Goldeneye's Xenia Onnatop deserves a nod) and was really scary, very strong and truly lethal. Then her boss, Max Zorin, made the mistake of betraying her and she meets her end by wheeling his bomb out of harm's way.
Boris Grishenko Boris wasn't really a henchman, but who can forget the crazy and loathsome hacker that betrayed his colleagues and joined forces with Alec Trevelyan? Skills: singlehanded typing while playing with a pen. He would often proclaimed himself invincible, but liquid nitrogen proved him wrong.
Nov 2008
nov 2008
Nov 2008
in association with
Bond VsBond
It is an argument older than time. Well, it sure does feel like it: who is the best James bond ever?
in association with
Sure, Daniel Craig is almost there, but he is hardly the icon that Connery is. Besides which, it is an undeniable fact that Sean Connery defined the role of James Bond in the first four films. Moore was too posh, Dalton too soft and Brosnan too unfocussed. Connery got the formula just right, knowing when to be a brutal killer and when to be a suave lover. He came across as charming and good looking, but there was never a moment that anyone would doubt Connery's Bond would kill you just as soon as look at you. And then let's take
a look at the films he acted in, and the villains he bested. Auric Goldfinger, Rosa Klebb, Dr No, Red Grant, Oddjob, Emilio Largo, Mr Wint & Mr kidd and, of course, Ernst Stavro Blofeld. These are the most notorious villains that Bond has ever faced, except maybe for Jaws, and Connery beat them all. There can be no argument: Bond, as Fleming intended him to be and as the world received him best, was and always will be Sean Connery. There would be no Bond without him, because he contributed to the success of the original films.
Written by Shahzad Sheikh Roger Moore (1973-1985) Recently Roger Moore is reported to have conceded that: 'I'm the worst Bond. Generally hated! I was too funny, too light. Didn't take it seriously enough.' But as he points out, 'this is a man who is supposed to be a spy. And yet he turns up in bars and hotels around the world, and everyone says, "Ah, Mr Bond, we've been expecting you."' Moore gave us an action hero perfectly in sync with the fantasy world of the big screen superspy. There were laser-guns and space shuttle dog-fights in Moonraker for goodness sakes, who else could have pulled that off? But let's look at the facts. Moore did seven Bond movies in 12 years making him the longest-serving Bond. When he finally stopped in 1985 with A View to a Kill he was 58. He wanted to quit after For Your Eyes Only in 1981, but was recalled for two more outings as 007. And it took ten years to find a good enough replacement when Pierce Brosnan starred in 1995's Goldeneye. By comparison Sean Connery only did six,
Aston Martin DB V
Grappling Hook Watch
Attache Case
X-Ray Glasses
The iconic Bond car and gadget, the Aston Martin DB V appeared in several Bond movies and featured bulletproof front and rear panels, oil slick sprayers, a smoke screen, machine guns, rotating licence plates, telescoping tire slashers, tracer receiving console and the famous passenger ejector seat.
Bond has had his fair share of cool wrist watches, but this one was a real lifesaver. Armed with a mini grappling hook and winch system, this watch allowed Bond to escape certain death from a mine.
This case was based on a simplified version from World War II. It contained a 3-piece sniper rifle, a knife in a sprung loaded compartment, ammunition, fifty gold sovereigns and, of course, a highly explosive tin of "talcum powder".
The suave Bond got more than an eyeful when donning these modified spexs. The glasses revealed concealed weapons to Bond but, in true fashion, he also got to check out some very lovely ladies sporting sexy lingerie.
Written by Walt Pretorius Sean Connery (1962-1971) You cannot beat an original. The concept of new and improved is just silly, particularly when it comes to a character as iconic as James Bond. When reading Fleming's books, Bond is a sophisticated killer, a hard drinking womaniser, a brutal near-psycho that does what it takes to get the job done.
No agent should leave home without these
nov 2008
if you don't count the aberrant Never Say Never, which wasn't an official production. It was released against Octopussy in 1983, but Moore's movie made more money. Ian Fleming's James Bond is English and apart from Daniel Craig, Moore is the only Englishman to have played him, and along with Timothy Dalton, is also the only RADAtrained actor. What's more it's rumoured that Fleming originally wanted Moore for Dr No, having been impressed with his portrayal of a Bond-like latter-day Robin Hood, from his hit 1960's TV series, The Saint. Good looking, tongue firmly in cheek and armed with deadly wit, it was Moore's Bond that set the template for the wise-cracking action.
Written by Matt Pomroy Timothy Dalton (1987-1989) Coming straight after a run of seven films with Roger Moore (and his safari suit) it was something of a shock when Timothy Dalton played Bond seriously. While podgy Rog looked like a lecherous lounge lizard, so Dalton looked and acted like the professional killer that James Bond is meant to be. This was as near to Fleming's creation as we've seen and indeed, Dalton read all the books to get a proper feel for the character and his traits. The ruthless realism was shunned by some cinema-goers who preferred to see the MI6 agent played for laughs with daft gadgets and corny lines, but those who appreciate the role, appreciated Dalton's performance. Likewise, a lot of the glamour had been taken out of his two films with his first outing seeing him battle through Bratislava, Gibraltar and Afghanistan rather than casinos and beaches, but he pulled it off. You could really imagine Dalton as a hit man: clinical, professional and ruthless in achieving his mission. Timothy Dalton achieved all this despite obstacles such as producers altering the tone and format, the public not being ready for his version of the character, a weak
script for his second film and the fact he was actually Welsh. Dalton was the right man – and a great Bond – just at the wrong time.
Written by James Francis Pierce Brosnan (1995-2002) When Pierce Brosnan arrived on the scene, Bond fans held their collective breaths. It had been six years since the last Bond movie – an unprecedented gap to this very day – all for good reason. There are many things that you can praise Dalton's Bond for, but he didn't have the magic touch to bring a new Bond in a post-Cold War world to life (And forget the schlock adventures of mad super-villains and secret criminal groups that characterized the eras before that). But Brosnan, with the equally-suave Remington Steele role under his belt, rose to the occasion. Goldeneye is still the movie that saved Bond, introducing a new kind of villain (the practical and deadly Alec Trevelyan) and a more sensible plot (even though it hearkened to the usual steal-the-money motives that drove movies like Goldfinger). It also introduced Dame Judi Dench as the new M – a fitting exclamation to the stronger feminine roles that appeared in the series during and just before the Dalton years. Tomorrow Never Dies dipped its toes back in the 'rich megalomaniac' bad guy pond, but with a more realistic flair, while The World Is Not Enough went straight for the implications of a spy's role in real-world affairs. The Bond plots were more realistic and yet more entertaining – Brosnan really drove the point home with a great blend of gentleman-meets-psychopath. Die Another Day, arguably one of the most disliked Bond films, was a clear throwback to the original cheesy plots and could be seen as a farewell to that style of Bond – opening the door for Daniel Craig's new version. Without Brosnan there would be no Bond today. It is as simple as that. Nov 2008
Published on Oct 7, 2010
A feature I wrote and produced about the James Bond franchise.


text:
Who we are
Doxim is a leading provider of customer engagement software for financial service organizations. We operate in an interesting space with a lot of growth potential - for our organization and our people. We partner with financial institutions to help them transform their client experience, communicate more effectively throughout the client lifecycle and improve cross sell and upsell activities that drive increased wallet share.
We promote a performance-oriented culture. And while to some that translates purely as the work done at your desk, here at Doxim, that translates to competitive laser quest tournaments, in-house pancake making breakfasts, and inter-office food bank collection competitions.
Located in Markham, Ontario, we are one of the fastest-growing technology companies in Ontario. We are expanding quickly, and are seeking a Senior Project Manager to help us reach the next level.
The opportunity
We need a Senior Project Manager to join our Professional Services team and lead large and small scale professional service project implementations for diverse clients in the financial services industry. You will be responsible for the full lifecycle of project engagements from scoping, initiation, tracking and reporting to closing and billing.
Who you are
You are part of a PMO or you would like to return to working as part of one. You're an experienced Project Manager with a proven track record of delivering projects on time, on budget and with high client satisfaction. In your career, you've successfully working on six-figure long term projects, for 9 to 18 months. Your experience may be with a service organization or a SaaS provider. If you are at a large-scale organization overseeing and implementing new projects, Doxim is the place to consider.
As our new Senior Project Manager you will:
The successful candidate has:
Why join Doxim
At Doxim, we've grown steadily over the last 15 years and accelerated our growth - we've tripled in size - over the past five. We aren't losing any momentum anytime soon. We work with hundreds of financial service providers to help improve their client communications, streamline document access, meet compliance requirements and reduce the expense, work effort and environmental impact associated with workflow, creation, and delivery of business critical documents.
We know that a happy work culture is an efficient, loyal and innovative team. Our senior leadership team is dedicated to offering mentorship and exposure to everyone that joins us. It's how we retain our team members for so long: we gain loyalty by providing our people the space and support to innovate, to be heard, to get credit.
To Apply:
Apply now, refer a friend, or stay on top of what's new by following us @Doxim_Inc.
Doxim welcomes and encourages applications from people with disabilities. Accommodations are available on request for candidates taking part in all aspects of the selection process.


text:
Choose Language
+386 1 563 69 59
Home Airline tickets Middle East Egypt offer from ROM, Rome
Flights Rome - EgyptSpecial offer
Did you know?
- There are 16 countries in the same zone as Egypt.
- The currency symbol for EGP is EGP.
- There are 3 carriers with direct flights from Rome to Egypt.
- There are 118 cities with flights to Egypt.
- Egyptair has the most flights to Egypt.
Hotels in Egypt
Traveling on the route and need hotel in Egypt? Choose from more than 45,000 hotels.


text:
The Maine Department of Inland Fisheries and Wildlife currently is accepting applications online for the Any-Deer Permit Lottery.
The Department will be issuing 45,385 permits this year to residents, non-residents, landowners, Superpack holders in these Wildlife Management District only: 15-17; 20-26, 29. It is "Bucks Only" in any Wildlife Management District not listed.
In an effort to reduce our costs, the Department no longer will mail paper applications beginning this year. Interested customers are strongly encouraged to apply online. The process is quick and easy, and applicants will get instant confirmation of their entry into the lottery.
The application deadline for persons applying at the Department or mailing in a paper application is July 31 at 5 p.m. Applications must be postmarked by July 31 or hand-delivered to us by 5 p.m. on July 31.
The application deadline for ONLINE applicants only is 11:59 p.m. on August 17.
The drawing will be held in September.
Applicants must possess a valid Maine license to hunt big game in order to apply or a valid Superpack license to apply for a Superpack Deer Permit. (This applies to Maine residents only.)
If an applicant does not have access to a computer or the Internet at home, we offer the following suggestions:
If those options are not available, please visit one of our 840 licensing agents and they will assist you in applying online. There is an additional $2 processing fee if you apply online through an agent.
If for any reason an applicant cannot apply online, we offer these options:
The address is:
Any-Deer Permit Application Request
Maine Department of Inland Fisheries and Wildlife
41 State House Station
284 State St.
Augusta, ME 04333-0041
If you applied for an Any-Deer permit last year, all of your information is pre-filled into this year's online application. To start, type in your first name, last name and date of birth as you printed it on last year's application. We'll look up your information. Please review your personal data and make any necessary changes. It's easy!
If you fill out an application this year - and happen to move or change your phone number before the application deadline - you can go online and edit your previously submitted Any-Deer permit application.
Once you've filled out your application, you'll be able to print out a confirmation page. Also, a confirmation will be e-mailed to you. This way you have a printed record of the day/time of your application as well as all of the information you provided to us.
Bonus Deer permits ONLY will be awarded in Wildlife Management Districts where there are more permits available than applications received. Last year, no Bonus Deer permits were available.
If you do not want to apply for an Any-Deer permit but would like to apply for a Bonus Deer should permits become available, make sure you designate a Wildlife Management District choice. No WMD changes can be made after the drawing.
For more information about the Any-Deer Permit Lottery, visit our website at www.mefishwildlife.com [2] and click on "2009 Any-Deer Permit Permit Lottery Apply Now" in the center area of the screen.
[1] http://www.addtoany.com/share_save?linkurl=http%3A%2F%2Fwww.biggamehunt.net%2Fnews%2Fmaine-any-deer-permit-lottery-deadline-august-17th&linkname=Maine%20Any-Deer%20Permit%20Lottery%20Deadline%20August%2017th%20%7C%20Big%20Game%20Hunt
[2] http://www.mefishwildlife.com
[3] http://www.biggamehunt.net/sections/Maine
[4] http://www.biggamehunt.net/sections/Whitetail_Deer


text:
orbitography or·bi·tog·ra·phy (ôr'bĭ-tŏg'rə-fē)
A diagnostic technique for radiographic evaluation in suspected blow-out fracture of the orbit.
Tagged: o
Read Also:
- Orbitonasal
orbitonasal or·bi·to·na·sal (ôr'bĭ-tō-nā'zəl) adj. Relating to the orbit and the nose or nasal cavity.
- Orbitonometer
orbitonometer or·bi·to·nom·e·ter (ôr'bĭ-tə-nŏm'ĭ-tər) n. An instrument for measuring the resistance of the eyeball as it is pressed backwards into its socket. or'bi·to·nom'e·try n.
- Orbitotomy
orbitotomy or·bi·tot·o·my (ôr'bĭ-tŏt'ə-mē) n. An incision into the orbit.
- Orbits
[awr-bit] /ˈɔr bɪt/ noun 1. the curved path, usually elliptical, described by a planet, satellite, spaceship, etc., around a celestial body, as the sun. 2. the usual course of one's life or range of one's activities. 3. the sphere of power or influence, as of a nation or person: a small nation in the Russian […]


text:
Stainless Steel 2.2mm 20" Round Box Chain Necklace
In Stock
Sold Out
We're sorry. This item is not available at this time.
Pre Order Possible .
Low Stock
A classy complement to any pendant, this round box chain necklace is also a smooth and stylish accessory on its own. It's handsomely crafted from stainless steel for an incredible look. From Steel by Design(R) Jewelry.
- Stainless steel
- 2.2mm round box chain
- Lobster claw clasp
- Measures approximately 20"L x 1/16"W
- Made in China
Delivery Date Estimate
Reviews & Community QA
Stainless Steel 2.2mm 20" Round Box Chain Necklace is rated out of 5 by 4.
Rated 3 out of 5 by jbolton from Not 20" long I ordered this stainless chain because I needed a sturdy yet low cost chain for a pendant I wear daily. I ordered 20" so that it would fit down in my uniform top, yet when I received the chain it was not 20" Yes, I measured it to be sure. So back to QVC it goes.
Date published: 2016-08-08
Rated 3 out of 5 by areece from unexpected I ordered the 20", but when I received the necklace it was very short. It is a little chunkier that I was expecting. Not sure if I will keep it or not. If I do, I'll give it to one of my daughters. Too short for me and I have had 20" necklaces before.
Date published: 2015-01-07
Rated 5 out of 5 by battitude65 from love it bought this for a cross I own, but the link on the chain will not fit through the bezel on the cross. But i really like the look of the chain and because it was inexpensive I have decided to keep it and look for another. My first steel by design purchase.
Date published: 2014-10-30
Rated 5 out of 5 by Binky 7 from Fantastic!!!! I've always loved the strength of a box chain, but the square chain doesn't look good when it twists on the neck. This has the strength of a box link, but because it is round it hangs perfectly! I've just ordered a second one . I wish they'd use different metals in this link...it is fantastic!!!!!
Date published: 2014-01-22


text:
LaSalle College geography department ranking
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Think about what career is best suited for you, and where you want to study. Request more information now.
Info concerning LaSalle College geography department ranking, and registering for elective degree programs. Which college degrees give you the best chances of finding employment after graduation? To earn a higher salary, compare accredited engineering concentrations, and research technical certificate programs in your chosen field. Concentrating on LaSalle College geography department ranking, for example, you may gain an advantage in job recruiting on-campus. Check with the urban planning department for course registration deadlines, and geography degree program requirements. Please use the form above to request admissions info for LaSalle College
Urban planning covers everything from ensuring water-supply networks, to designing municipal solid-waste management and disposal. In the case of underground utility networks, it also includes maintaining conduits and access of electrical and telecommunications cables. Municipal engineers may also design civil projects including large buildings, industrial plants, or treatment facilities. Transportation engineering focuses on the construction and maintainence of streets, highways, and mass transit systems. Urban planners averaged $80,500 annually in 2017, much higher than in many other occupations.
Surveyors may be called in to lay out the routes of railways, roads, and canals before construction begins. New advances in Global Positioning Systems (GPS) allow high-precision surveys down to the square foot, a greater resolution than was previously available. Although surveying is a distinct profession with separate qualifications and licensing arrangements, civil engineers must be trained in the basics of mapping, as well as geographic information system (GIS). Beyond major requirements within the urban planning department, you may consider Minot State thermodynamics as an adjunct to your primary course of study.
Field Process Engineer
Plasma-Therm LLC
Provide technical support and trouble shooting on Etch and Deposition products in the field to customers, field service engineers, and the process engineering..
2 days ago Automation Engineer
Integrated Resources
Ability to read and understand electrical drawings, particularly controls portions. Supports equipment automation, information collection and reporting, and..
2 days ago Electrical Engineer
Louisville Gas & Electric
Bachelor degree in Electrical Engineering. Assists other engineers with large and/or complex projects, as required.
5 days ago Construction Project Engineer
Top Notch Talent Management
Project Engineer Job Duties. Develops project objectives by reviewing project proposals and plans..
2 days ago Experienced Electrical Designer
Hawkins Design Group
We are seeking a skilled/experienced electrical designer knowledgeable in power, lighting and HVAC electrical system design for producing building construction.
5 days ago PreSales Solutions Engineer (Remote / Telecommute)
Is seeking a seasoned PreSales Solutions Engineer with experience within the supply chain or retail arenas to do the following..
4 days ago


text:
Caged Bib Neck Crop Top
Standard Price $9.99 Sales Price $2.99
Select size
Details content below
Product Model Size:Model is 5'9" tall
Thin straps stack up the sides, to make this crop top extra edgy and extra caged! Soft knit stretches into a figure-flattering fit, with a bib neckline to top it off. Add some more edgy style with any of our caged pencil skirts or destroyed denim shorts!
Returns content below
Return or exchange in any Charlotte Russe store within 30 days of your ship date, or use the mailing label included in your package to send it back right away.
We do not accept returns on jewelry, swim, intimates, nail polish, fragrance, gift cards, opened beauty products or anything marked as final sale.
You Might Like These Too
Style: 302366521


text:
blood and it is all clear of hydrocodone
When u stop taking hydrocodone how long does it take before it is out of your system to take your?
- Asked
- 1 Sep 2010 by dkopach
- Active
- 27 Oct 2012
- Topics
- hydrocodone, blood disorders
Responses (1)
Further Information
- Hydrocodone Information for Consumers
- Hydrocodone Information for Healthcare Professionals (includes dosage details)
- Side Effects of Hydrocodone (detailed)
Search for questions
Still looking for answers? Try searching for what you seek or ask your own question.
Similar Questions
Stop taking hydrocodone, How long after will it take to leave my system?
Posted 16 Nov 2010 • 1 answer
How long does Metoprolol stay in your system after you stop taking it?
Posted 14 Jul 2011 • 1 answer
How long does hydrocodone stay in your system for a blood test?
Posted 1 Oct 2011 • 5 answers
ExeFen-IR - How long does it take for exefen ir to get out of your system once you stop taking the?
Posted 28 Jan 2013 • 1 answer
Menopausal Disorders - stop taking estridol..what can I expect?
Posted 1 Nov 2014 • 1 answer


text:
The cashier with blonde hair didn't give me back my change. A few cents would be fine but it was 82 cents. At least ask the customer if they do or do not want the change, don't assume. Will not be...
3696 SW Topeka Blvd
Topeka KS 66611
Topeka KS 66611
(785) 266-4520 Website
See Availability
Sponsored Topics
Opening Hours
- Mon: 8am-10pm
- Tue: 8am-10pm
- Wed: 8am-10pm
- Thu: 8am-10pm
- Fri: 8am-10pm
- Sat: 8am-10pm
- Sun: 8am-10pm
See a problem? Let us know!


text:
JCM Global has signed some significant orders for its iVizion bill validator solution.
Three casinos will now take advantage of the product. The locations are in Bilbao, Ceuta on the north African mainland, and the island of Mallorca.
The casinos, all from the Luckia Group, will replace their existing solutions with iVizion. Said JCM's general manager EMEA: "These casinos will now experience unparalleled levels of protection against counterfeiting and will receive high-speed service for their customers."
IVizion has strong defences against counterfeits and "stringing". Its Contact Image Sensing technology scans 9.5 mllion data points on every bill – twice that of its nearest competitor.


text:
Essay writing an outline
College Essay Outline Format Ideas and tips to help students outline their college admission essays.Sample Scholarship Essay Outline. I. Introduction a. A basic guide on how to make a good essay outline. Learn how an essay outline can help you structure a great essay. Just follow this simple but effective descriptive essay outline AceWriters - excellent solution for writing your essay! Organization Basic Essay Format.PDF. Learn about essay outline format and essay outline structure, review essay outline template, essay outline sample and reserch outline sample. We provide cheapest essay writing service for all learners. 100% confident and high quality service. Order essays from us and you will see how much we appreciate our. Essay Outline Writing Service Lookingistake every "write my essay" request seriously and do the best job on your essay, term paper, or research papers. This resource begins with a general description of essay writing and moves to a discussion of common essay genres students may encounter across the curriculum.
Writing An Outline For An Essay Writing an Essay Outline Purpose. Writing an essay outline helps organize your information. This will help you build a rough idea of. Blank Essay Outline The Basic Outline of a Paper The following outline shows a basic format for most academic papers Free writing tips for outline writing. Our essay help online service really believes in successful meeting the most strict deadlines our clients have every student day! Rely upon our talented team. Definition. An outline is a plan for--or a summary of--a writing project or speech. An outline is usually in the form of a list divided into headings and. Writing a Compare/Contrast Essay The following example contains an element of the author's opinion, but not all compare/contrast assignments allow for that. Term Paper Writing Outline Writing an essay takes a lot of time. Students are not able to cope even with the essay outline, which is the first step in writing an essay. Basic Essay Help With Writing: Writing Guides Techniques, Prompts, Citation Samples and Tips. We help students improve their basic essay writing skills. Essay writing assistance is now available. Buy essay – any topics and subjects, for all academic levels are written by professional and experienced writers. Writing Essays by Eleanor Wakefield There are several vital elements to any successful college essay. This handout will define those elements and show you how to put.
Essay writing an outline
Writing research paper outline examples is an effective and. Critical Literary Essay Example.help writing a research paper outline. Essay Celebrating. Don't worry—consider me your architect. I'm here to give you an analytical essay outline that'll make writing the final draft (relatively) painless. Help Writing An Outline For An Essay Essay Writing Help and Essay Basics. Our web resource offers essay help to those who are looking into improving their writing skills. It's more easier to ask for paper writing help from our custom paper writing service when trying to meet all assignment strict requirements and deadlines. Essay Writing Outline Template SampleSubject: Essay Writing Keywords: Thesis, Introduction, Body Paragraphs, Conclusion Created DateEssay Outline Template I.
Outline for Essay. Writing an essay outline can be hard. In truth, creating the outline for your essay can be done easily and in a few minutes. The essay writing industry is a source of interesting statistical data. California, New York and Texas are the most popular regions where orders were. Information on all Essay Types, Stages of Writing, Common Essay Subjects, all Academic Levels and Formatting Styles. Writing tips, common mistakes and problems in. Outline Of An Essay 5-Paragraph Essay and Outline The Hazard of Moviegoing Essay Sample. The Hazards of Moviegoing. By John Langan From College Writing Skills with. Developing the outline for your essay helps your organize your ideas to tell a story. The outline is a fast and easy way to get your thoughts on paper. Are you in High School, College, Masters, Bachelors or Ph.D and need someone to help write your essay?Outline for Essay. Writing an essay outline can be hard.
Developing Outline; Writing Drafts; Editing and Proofreading;. In this section you will find samples of essays belonging to various essay types and styles of. Help Write Essay offers more than just essay help online. We deals with all kinds of student tasks from simple essays and case studies to research and term. Often people, who are coming to ask "write my essay for me" for the first time, are not really aware of how to do this properly and how to avoid the misunderstandings. Our essay guidelines will help you to become a super writer! More tips and instructions inside. Order your essay on Guidetoessay.com. Trying to devise a structure for your essay can be one of the most difficult parts of the writing process. Making a detailed outline before you begin writing is a. Outline Structure for Literary Analysis Essay I. Catchy Title II body of your essay, use examples and fully developed logic to prove that the literary. Cause and effect essay outline - Fast and reliable services from industry best agency. Get an A+ help even for the most urgent writings. confide your dissertation to.
Creating Outlines. Outlines can be a helpful tool when you're trying to organize your thoughts for an essay or. What is my main point or purpose in writing this. Once you break it down, writing an argumentative essay outline isn't that daunting. Download this skeleton Argumentative Essay Outline to get started. Here you can find information on how to write an essay outline. Aldo you can get help at ProfEssays Essay Outline help team. Outline Persuasive Essay Persuasive essay on immigration outline. There are criminals bullying. Youve decided to tackle your stance and natural solution arthur d. Essay writing in elementary, middle and high school is easy if you know the steps to follow: create an outline, thesis, introduction, body and conclusion.
Essay Map - ReadWriteThink. Outline Essay Writing These sample outlines will help students organize their ideas before writing an essay, a good way to ensure less time is spent in revision. Writing a good introduction to an essay can hook the reader, creating a desire for more. That's why it is important to craft the best introduction possible. Every essay outline follows the same basic formula and learning how to structure and write an essay can be easy if you follow the outline formula. Help with essay writing uk essay on laziness never pays analysis homework help United States Writing Agency. We will. Division Business Plan Outline.
Essay My Mother 5th ClassHow To Write Essay In Ielts Exam Outline For Writing A Five Paragraph Essay Gre Essay Prompts List. Essay On Progressive Reform Movement. This is the most common type of outline and. The sample PDF in the Media Box above is an example of an outline that a student might create before writing an essay. A classic format for compositions is the five-paragraph essay. It is not the only format for writing an essay, of course, but it is a useful model for you to keep in. 5-Paragraph Essay and Outline "The Hazard of Moviegoing" Essay Sample. The Hazards of Moviegoing. By John Langan From College Writing Skills with Readings. Writing and essay outline - The Leading Student Writing and Editing Help - Get Professional Help With Custom Written Paper Assignments Quick Cheap Essay And Research. Writing A Essay Outline Open education resources for academic writing Writing a Paper Outlining Writing a Paper: Outlining. making an outline of each.


text:
"When we were young us girls practiced kissing each other because we wanted to be good at it if we ever got boyfriends. Now I know instead of kissing we should have been practicing saying goodbye."
No, this isn't an entry on one of those secret confession websites. It's one of the titles of the paintings by self-taught artist Andrea Heimer, which will be featured in mid-June at an exhibition at Shooting Gallery.
Called "Sex in Suburbia," the pieces in the show are influenced by Heimer's childhood in the suburbs of Montana.
"I got this show by sending Shooting Gallery a post card saying I'd always wanted to work with them," said Heimer in an email to SF Weekly.
As Heimer explains on her website, she "paints the weird/dark/hidden sides of the suburban landscape." The titles of her paintings aren't so much titles as they are stories, such as "The Block Party of 1984 Went Down in Neighborhood History Because Otto Johnson Tried to Get Lilly Watson Pregnant Without Her Permission and Nobody Noticed." As the name of the show suggests, many of the titles of the featured paintings include references to sex, and also suicide, family surnames, street names, sex, and something called "The Sunshine Cult."
In addition to paintings, Heimer also makes sculptures of people and animals, most of which have a quilted appearance while still being as dark as her paintings.
"Sex in Suburbia" opens June 14 at 6 p.m. and runs until July 5.
Comments are closed.


text:
Synthesis of copolyfluorenes with high fluorenone contents and its application in electroluminescent device by simple blending
Version of Record online: 9 SEP 2010
DOI: 10.1002/app.32705
Copyright © 2010 Wiley Periodicals, Inc.
Journal of Applied Polymer Science
Volume 119, Issue 5, pages 2576–2583, 5 March 2011
Additional Information
How to Cite
Wu, J.-R., Chen, Y. and Wu, T.-Y. (2011), Synthesis of copolyfluorenes with high fluorenone contents and its application in electroluminescent device by simple blending. J. Appl. Polym. Sci., 119: 2576–2583. doi: 10.1002/app.32705
Publication History
- Issue online: 1 DEC 2010
- Version of Record online: 9 SEP 2010
- Manuscript Accepted: 25 APR 2010
- Manuscript Received: 12 FEB 2010
Options for accessing this content:
- If you are a society or association member and require assistance with obtaining online access instructions please contact our Journal Customer Services team.
- If your institution does not currently subscribe to this content, please recommend the title to your librarian.
- Login via other institutional login options http://onlinelibrary.wiley.com/login-options.
- You can purchase online access to this Article for a 24-hour period (price varies by title)
- If you already have a Wiley Online Library or Wiley InterScience user account: login above and proceed to purchase the article.
- New Users: Please register, then proceed to purchase the article.
Login via OpenAthens
Search for your institution's name below to login via Shibboleth.
Registered Users please login:
- Access your saved publications, articles and searches
- Manage your email alerts, orders and subscriptions
- Change your contact information, including your password
Please register to:
- Save publications, articles and searches
- Get email alerts
- Get all the benefits mentioned below!


text:
Registration for Winter Intro Class
Our Winter Intro class registration is open! Join our Northside Chicago class and for 8 weeks learn a new, dynaminc sport, make friends and have fun burning off those holiday calories!
The class session starts Thursday, January 5 - Youth (8 - 12) at 5:30, Teens & Adults at 7 PM. To reserve a spot in the class or for more information about the club Contact Us.


text:
About Sophion Technologies
Sophion is dedicated to providing state of the art products and integrated solutions for automated patch clamping. The patch clamp technique is a laboratory technique in electrophysiology that allows the study of single or multiple ion channels in cells.
Find out more below about the patch clamp technique and our chosen technological path to secure reliable ion channel recordings. Please see below.
Ion Channel Screening for Non-Experts
Sophion provides high tech ion channel screening solutions. You can read more about ion channels below.
Ion channels consist of proteins and are embedded in all cell membranes of all living organisms. They are responsible for electrical signaling within cells and between cells. The signal is carried by ions (i.e., 'salt') that normally cannot pass the cell membrane but can diffuse passively (no energy expenditure) through ion channels, when they open in response to certain conditions. This electrical signaling is crucial for all physiological processes.
Examples of such processes are:
- Generation of electrical activity in nerves
- Control of contractile activity in the heart and muscles
- Nutrient uptake
- Hormone secretion
There are many ion channels
A typical cell has 100-1000 ion channels of different types. Most ion channels allow only one specific ion to permeate through them. This is reflected in the common names of the ion channels, e.g., K+, Na+, Ca2+ and Cl– ion channels. The sequencing of the human genome has recently led to the identification of more than 400 putative ion channels. Only about 100 of these have been cloned and functionally tested so far.
Ion channels play a role in many diseases
A number of human diseases, including pathological pain conditions, epilepsy, cystic fibrosis and a variety of neural and muscular disorders, are caused by defects in the function of ion channels.
Many drugs affect ion channels
The large number of physiological processes regulated by ion channels and their role in many diseases make ion channels highly interesting as targets for new drugs. Today, about 20% of all registered drugs target ion channels. The drugs modulate specific ion channels, resulting in altered cellular behavior.
Ion channels are difficult to explore
Ion channels are important targets in drug discovery, but they are also difficult to study. Despite significant research efforts, there is still limited knowledge about ion channel function and how ion channels are related to specific diseases. This is mainly a result of the limitations and complexity in the technologies that are available to ion channel research. Ion channels are therefore considered to be largely unexploited compared to other target classes.
Methods to explore ion channels
There are two main methods to investigate ion channels: direct and indirect methods. The only direct method is called 'patch clamp'. In brief, patch clamp is very accurate, but also very time consuming (i.e., has low throughput), while the indirect methods are less accurate, but typically much faster.
Patch clamp
The patch clamp technique is considered the gold standard in ion channel research. The technique was developed in the 1970's by Erwin Neher and Bert Sakmann, who received the Nobel Prize in Physiology and Medicine (1991) for their work. In a traditional patch clamp experiment, a tiny glass pipette containing an electrode is attached or 'sealed' tightly to the cell membrane. The cell membrane is ruptured manually by applying suction through the glass pipette. The tiny currents (10-12-10-9 amperes) through the ion channels can be measured via the electrode, which is connected to an amplifier. The typical throughput is three to ten successful patch clamp experiments per day and requires patience and high-level insight in electrophysiology.
Indirect methods to study ion channels
There are several indirect methods for studying ion channels. The most important indirect methods are based on fluorescent dyes that are loaded into cells and then analyzed using specialized plate readers. The readers detect a change in the concentration of certain ions that is the result of ion channel activity or a change in the voltage across the cell membrane, also caused by the flow of ion through the channel. The main benefits of these methods are their high throughput and low cost per data point. The main limitations of the methods are low accuracy and sensitivity, and lack of control of the voltage across the cell membrane, making it possible to study only certain types of ion channels and rather simple ways of drugs interacting with the ion channels.
Automated patch clamp technologies
QPatch is Sophion's product family for medium-throughput automated patch clamp, and Qube is the newest high-throughput automated patch clamp system. With the QPatch and Qube, patch clamp experiments are done on a planar 'glass' surface that creates a connection between the cell and the amplifiers that are very similar to the manual 'gold standard' patch clamping. QPatch and Qube operate automatically and these systems increase patch clamp throughput due to the high degree of parallelism.
QPatch and Qube therefore combine the accuracy of traditional patch clamp with the high throughput of indirect methods.
Pharmaceutical companies developing ion channel-based drugs have a great demand for automated patch clamp systems that can quickly deliver accurate results.
The drug discovery process in short
The drug discovery process in pharmaceutical companies can begin either with a certain target, e.g. a specific ion channel, or a certain disease and a search for relevant therapeutic targets to modulate. One important class of targets comprises ion channels. After a target validation phase, a test system (an assay) is developed to measure interactions with the target. Pharmaceutical companies have huge libraries of chemical compounds, the largest of which contain up to a couple of million compounds. One or more of these compounds may be identified as so-called hits with a desired effect on a specific target. From this hit, chemical modifications can make the identified molecule suitable as a drug. This process is iterative and called lead optimization. For every modification, new patch clamp tests are needed to check that the desired effect is preserved or improved. Further tests in disease relevant animal-models and safety test are carried out before the development in the clinical trials (test in humans) can lead to an approval by health authorities to market a new drug.
Finding the hits is like finding a needle in a haystack! For the last 20 years it has been routine to test either the entire compound library, or part of it, with indirect methods in a so-called High Throughput Screening (HTS) department in the pharmaceutical company that identifies the initial hits.
With Qube and downstream—The drug discovery revolution
The very low throughput of traditional patch clamp technology means that this technology is currently only used in the initial target identification and validation phases and in the final steps of lead optimization.
The process of ion channel drug discovery changes dramatically when automated patch clamp systems, such as QPatch and Qube, are employed systematically. Qube uses the 384-well format, which makes it suitable for the HTS segment, and QPatch has the high flexibility that makes it ideal for lead optimization. Qube performs direct electrophysiological tests and hence identifies more interesting hits with higher validity than the indirect methods. As a result, the hit validation can be shortened significantly or even eliminated and still identify lead compounds of higher quality. The result may be the development of more and also more efficient ion channel-based drugs, with a 20–50% shorter pre-clinical development timeline.
More and better leads—faster. That's why we say that the combination of Qube and QPatch is leading a revolution in drug discovery.
Ion Channels
QPatch and Qube are an automated patch clamp system for high-throughput electrophysiology measurements on ion channels. You can read more about ion channels below.
Ion channels are targets for selective drugs
Ion channels constitute a large and diverse group of membrane proteins that function as electrical signal transducers, and they govern the electrical properties of all living cells. For example, the coordinated activity of several ion channels underlies action potentials in excitable cells, such as those in the heart and brain.
Ion channels are generally heteromultimeric membrane proteins that constitute water filled passageways for ions across the phospholipid bilayer membrane. The physical pore is shaped by an assembly of several subunits, and the pore is lined with hydrophilic amino acid residues. A narrow region of the pore constitutes a 'selectivity-filter' that determines which ions can pass through the pore.
Ion channels may open and close in response to e.g. membrane potential (voltage-gated ion channels) or chemical (ligand-gated ion channels) stimuli.
Classification of Ion Channels
Each ion channel is characterized by its ion selectivity sequence. It may be highly specific for a single ion, or it may be less specific, conducting a few or several different ions. The selectivity is reflected in the common classification of the channels:
- K+ channels
- Na+ channels
- Ca2+ channels
- Cl– channels
- non-selective cation channels
Functionally, ion channels are broadly divided into voltage- and ligand-gated channels, referring to the type of physiological stimulus that activates the channel.
Diseases linked to Ion Channels
A multitude of human and animal diseases are caused by ion-channel dysfunction. This may be genetic, i.e. caused directly by mutations in genes coding for ion channels. Such diseases are called 'channelopathies'. Examples of channelopathies are cystic fibrosis, epilepsy, and cardiac arrhythmias, e.g. the long QT syndrome. Diseases may also result from defects caused by mutations in genes coding for proteins that regulate ion channels.
Alternatively, ion channels may be involved in non-genetic diseases, e.g. diarrhea, which is mediated by toxicological effects on ion channel function.
Ion channels as drug targets
The search for new, potent and selective drugs that interact with specific ion channels is strongly technology driven and focused on high-throughput screening. Active substances from these high-throughput screens are further analyzed in functional studies, such as patch clamp. This development towards screening at the molecular level has been enabled primarily by: 1) the cloning and expression of relevant ion channels in cell lines, and 2) novel biological high-throughput screening techniques.
Technologies for ion channel characterization
The only direct way of validating the effect of a chemical entity on an ion channel is to measure the ionic current through the channel and determine whether the compound causes a change in this current. The patch clamp technique
has proven to be extremely useful in revealing many aspects of ion channel function. However, the traditional patch clamp technique has serious shortcomings in pharmaceutical discovery and screening, because the throughput is low, and it requires highly specialized personnel.
With the advent of automated patch clamp equipment these shortcomings are largely eliminated. QPatch and the Qube allow an operator without prior electrophysiological knowledge to conduct experiments. You can be a patch clamper too, with the help of the highly skilled application scientists at Sophion, or a skilled electrophysiologist in your staff!
Patch Clamping
QPatch and Qube are automated patch clamp system for high-throughput electrophysiology measurements on ion channels. You can read more about the patch clamping technique below
The Patch Clamp Technique
Since its introduction by Professors Bert Sakmann and Erwin Neher in the mid 1970's, patch clamp has been the classic method for studying ion channel function. It allows direct measurement of single channel currents as well as of the total current across the entire cell membrane. A glass micro-pipette, containing an electrolyte and an electrode, is pressed against the cell membrane, and a piece of membrane (the 'patch') is positioned within the pipette orifice. A tight seal of gigaohm electrical resistance (a 'gigaseal') is formed between the pipette rim and the cell membrane. If the patch contains ion channels then movement of ions through these channels is measured as tiny (picoampere) currents. The leak current across the seal is insignificant due to the high resistance of the gigaseal.
Specifically, five configurations may be employed:
- Cell-attached (on-cell): the pipette makes a gigaseal with the intact cell, allowing measurements of single-channel currents.
- Inside-out: cell-attached configuration is achieved and the pipette is withdrawn while the gigaseal is maintained, rupturing the cell. The inside (cytoplasmic) side of the membrane faces the bath fluid. This configuration is used for single-channel recordings with the ability to change the 'intracellular' solution.
- Whole-cell: the cell-attached configuration is achieved and vigorous suction is applied to the pipette, causing the patch to break. The cytoplasm and the pipette solution are subsequently in direct contact. After a short time, diffusion of cytoplasmic constituents (molecules and cell organelles) leads to identical (unphysiological) chemical composition of the fluids in the cell and in the pipette. The activity of all membrane ion channels is measured in this configuration.
- Outside-out: the whole-cell configuration is achieved and the pipette is gently withdrawn. This causes the membrane to break outside the sealing zone. The membrane fragments subsequently flip over, reseal, and constitute an inverted membrane patch exposing the extracellular side to the bath fluid. This configuration is used for single-channel recordings.
- Perforated whole-cell: the cell-attached configuration is achieved with pore-forming compounds (e.g. amphotericin B, nystatin) in the pipette solution. This causes perforation of the patch allowing small molecules and ions, but not larger compounds, to cross the patch. Consequently, larger molecules and cell organelles remain within the cell. The sum of all ion channel currents is measured, as with conventional whole-cell patch clamp.
Patch clamp has proven particularly powerful In combination with molecular biological techniques: specific ion channels may be expressed in cultured cell lines allowing a thorough characterization of their biophysical and pharmacological properties by patch clamp.
Patch clamp technologies for high-thoroughput screening
Unfortunately patch clamp is a low throughput technique. It is time-consuming and demands a skilled operator. The QPatch family and the Qube systems enable automated parallel ion channel screening with throughputs of hundreds or thousands of data points per day, enabling faster, and more accurate drug discovery.
Our Technologies
The sophisticated automated patch clamp systems Sophion provides for high throughput electrophysiology measurements on ion channels is based on three components:
- Patch hole micro technology
- Patch clamp amplifier
- Assay software
These are critical to obtaining high-quality results, under control and with high throughput. You can read more about these technologies below.
Patch hole technology
We develop and manufacture the consumables for our instruments: QPlate, for use with QPatch, and QChip 384, for use with Qube. The heart of the consumables is the silicon-based patch hole, which replaces conventional glass pipettes. This is an advanced and exclusive technology developed and owned by Sophion. The patch hole enables the routine formation of genuine gigaseals, ensuring uncompromised data quality.
A unique feature of QPlate and QChip 384 is the compound application principle, which is based on an advanced microfluidic flow channel that leads compounds and buffers to the cell. Each site has its own flow channel that enables both fast and reproducible liquid exchange, effectively increasing throughput and reducing the cost per data point.
Furthermore, the measurement site is based on a sophisticated combination of glass and silicon that avoids problems with 'sticky' compounds. This makes our consumables ideal for experiments with both ligand-gated and voltage-gated ion channels.
Finally, the embedded silver-chloride electrodes ensure superior voltage control, reduce risk of cross contamination, and completely eliminate the need for maintenance.
Patch clamp amplifier
Sophion develops custom-made patch clamp amplifiers in-house. These feature automatic compensation for series resistance (Rs), cell capacitance (Cslow), and up to 300 nA maximal current measured, depending on amplifier type. This specification contributes to very high quality recordings that are regarded as a hallmark of Sophion products. We believe that amplifiers are so vital in obtaining reliable and reproducible ion channel recordings that we need full control, by in-house development utilizing the accumulated experience of our experts. We are convinced that our in-depth understanding of how amplifiers should be integrated into the patch clamp instrument means that we can offer the best amplifier solution on the market.
From the platform of the screening station, a 'bed-of-nails' connects the patch clamp electrodes directly with the amplifier front ends. This distance is critical and minimized to reduce any noise pick-up from the surroundings.
Several parallel patch clamp amplifiers are implemented on a standard printed circuit board, enabling all amplifiers to be positioned near the patch clamp electrodes. Due to their small size, these amplifiers are ideally suited for extensive parallelism and provide considerable scalability.
Sophion products contain a large amount of software, including robotic control software, embedded software and user-facing application software. Our software is developed by a dedicated team of software developers who are experts in their respective fields, such as signal processing, statistical data analysis, robotics, and user interfaces, and also have an in-depth knowledge of the application field – automated patch clamping. Through daily collaboration with our customers, we have gained valuable insights to the research, protocols, quality parameters, work flows and procedures used in electrophysiology groups and HTS labs. Sophion software is under continuous development, always with the final user in mind.
Advanced data analysis
Large patch clamp data sets are easily obtained with our instruments, necessitating rapid and automated data handling. Our Assay Software leads in the industry as it provides advanced data analysis on hundreds or thousands of experiments simultaneously, and maintains the high information content characteristic of patch clamp experiments.
CiPA – Comprehensive In Vitro Proarrhythmia Assay
Biolin Scientific, a CiPA partner, has a long-standing interest and extensive experience in cardiac ion channels. Our instruments are used for in vitro safety screening by major pharmaceutical companies and CRO's worldwide and we are ready to assist you in setting up your CiPA assays.
Brochure: All this talk about CiPA
Article: CiPA assays on QPatch
Article: MICE Model in Drug safety using QPatch
The Comprehensive in vitro Proarrhythmia Assay (CiPA) is a proposal by the FDA, HESI, CSRC and SPS ultimately aimed at revising the non-clinical ICH-S7B and replacing the clinical ICH-E14 guidelines. The S7B and E14 guidelines were implemented 2005 in response to reports of certain drugs causing TdP and these guidelines have been successful in the respect that no drugs have been withdrawn from market for being proarrhythmic after 2005. However, the connection between proarrhythmia and QTc prolongation is complex and depends on several other factors in addition to drugs blocking the hERG channel and while QTc prolongation is a sensitive marker for proarrhythmia it is also moderately specific (i.e. torsadogenic compounds prolong QTc but not all QTc prolonging drugs are torsadogenic).
The intention of the CiPA proposal is to increase the efficacy of the drug development process by 1) moving the evaluation of proarrhythmic risk to an earlier stage in the drug development process, and 2) enabling compounds with properties that today are considered as problematic to be further developed and 3) provide a stronger scientific foundation for improved future drug labeling. The CiPA proposal is based on a mechanistic understanding of proarrhythmic risk and is built around a three-component process:
- candidate drugs are tested in multiple and standardized ion channels assays using overexpressing cell lines. This proposal includes Nav5 (peak and late currents), Kv4.3 (Ito), hERG (IKr), KvLTQ1/mink (IKs) and Kir2.1 (IK1),
- the data from the ion channel assays are used in a computational model of a cardiomyocyte action potential model to see if the compound yields proarrhythmic markers. This model is calibrated using data from well characterized reference compounds,
- the results from the in silico simulations are verified using iPS derived cardiomyocytes
The FDA has proposed a revision of the S7B by June 2016 but there are still many questions regarding protocols, validation, translation and more that still awaits answers. and it is difficult to say when it is finally implemented.
Biolin Scientific is a CiPA partner and has a long-standing interest and extensive experience in cardiac ion channels. Our instruments are used for in vitro safety screening by major pharmaceutical companies and CRO's worldwide and we are ready to assist you in setting up your CiPA assays.
CSRC Cardiac Safety Research Consortium
FDA Food and Drug Administration
HESI Health and Environmental Science Institute
SPS safety Pharmacology Society
TdP torsade de points
Read more about CiPA on Eureka: The Heart of the Matter and on HESI


text:
Virtu USA KSC700ZG
KSC-700-ZG Dior 18 Modern Side Cabinet in Zebra Grey
The Virtu USA 18" Dior - Zebra Grey - Side Cabinet represents a modern design with clean sleek lines. This side cabinet will complement any Dior series vanity set to provide variable sizes. Available at AppliancesConnection
- Cabinet only dimensions: 18" W x 18.1" D x 29.5" H
- Zebra Grey Finish
- Made with the finest veneer, meticulously crafted to ensure the best edge banding
- Water Resistant Low V.O.C Sealer
- Adjustable hinges and slides
- 4 Doweled Drawers with Concealed Soft Closing Slides
- Polished Chrome Hardware with Satin Nickel Highlights
- No Assembly Required
- 2 Years
- Virtu USA reserves the right to repair, replace or refund any products resulting from a manufacturer's defect.
Be the first to ask a questionAsk A Question
Be the first to review this productWrite A Review
- Brand:Virtu USA
- Stock Id:396605
- Product Number:KSC700ZG
- Finish:Zebra Grey
- Installation Type:Freestanding
- Country of manufacture:China
- Basin Color:White
- Counter Top Category:Stone
- Short Counter Top Material:White Marble
- Weight:90.00 lbs