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Men's New Utilizer™ Polo
Detailshttp://www.columbia.com/mens-new-utilizer-polo-AM6215.html AM6215
$34.99 $50.00
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Style# 1441431
Greater Rewards members earn 350 points with purchase
Uses: Travel
Size & Fit Guide | Reviewers rated this product True to Size | Fit Modern Classic
- 1. Select Color:
- White Out of stock
- Black
- Graphite Heather
- New Cinder Heather
- Surplus Green Heather
- Phoenix Blue Heather
- White
- Collegiate Navy
- Red Velvet
Product Actions
Style# 1441431
Uses Travel
This stalwart performance classic features a clean design with long, streamlined cut and a super-wicking, UPF 30 fabric that feels great against the skin and has a lovely hanging power.
- Omni-Shade UPF 30 protects you during long hours in the sun
- Omni-Wick fabric actively breathes and pulls moisture away from the skin
- Underarm gusseting for comfort and mobility
- Modern Classic fit
- Imported
- 100% Omni-Shade UPF 30, Omni-Wick polyester two-tone pique
High-Performance Wicking Fabric
High-Performance Wicking Fabric
Sun Protection
Sun Protection
Technology Videos
Pulls moisture away from the body so sweat can evaporate quickly.
Blocks UVA and UVB rays to help prevent sunburn and long-term skin damage.
Men's New Utilizer™ Polo is rated 4.4 out of 5 by 73.
Rated 5 out of 5 by Hose from Great Polo These are the best polos I've ever bought. They are moisture wicking and are keeping me cool while working outside. The material is soft and come perfectly wrinkle free out of the dryer.
Date published: 2017-09-14
Rated 5 out of 5 by PastorScott from Love my polos The polos are some of the most light and comfortable shirts I have ever owned.
Date published: 2017-09-02
Rated 5 out of 5 by Rahasyamayi from True Fit and perfect for travel or leisure I have had this polo for about a month and I am completely satisfied. This is perfect fit and functions well indoor and outdoors, a great wear for summer especially
Date published: 2017-08-02
Rated 5 out of 5 by Outdoorsmostly from polo shirt light weight, wicking, very comfortable to wear all day.
Date published: 2017-07-24
Rated 5 out of 5 by WhatMine from Impressed Very nice!! Just as it was described.
Date published: 2017-06-28
Rated 2 out of 5 by lava from Utilizer Polo Good shirt and liked the color. The only fault is a defect in the sewing on the shoulder. Contacted the company with an option to send it back, but I find that is always a pain to do. (This review was collected as part of a promotion.)
Date published: 2017-06-19
Rated 5 out of 5 by Lotse153 from Great Polo I love these polo shirts. They do not stain like the all cotton shirts and they seem to wick moisture away from the body. I hope to add to my wardrobe again soon.
Date published: 2017-05-28
Rated 5 out of 5 by Nueton from Very nice This product is great for the price. It's easily wicks away sweat on hot days and it's simple design goes with anything. I highly recommend it.
Date published: 2017-05-03
Rated 1 out of 5 by HersonB from disappointing it doesnt look how it is shown
Date published: 2017-04-19
Rated 5 out of 5 by Lili85 from Excelen quality Nice quality and nice design. Happy with this purchase (This review was collected as part of a promotion.)
Date published: 2017-04-18
Rated 5 out of 5 by zach23 from great shirt Bought this shirt for work fits perfectly and is very comfortable.
Date published: 2017-04-12
Rated 5 out of 5 by IsleSeat from Comfortable, durable, good looking shirt Love Columbia Utilizer Polos. They are comfortable, fit and wear extremely well. Because they are light and resist wrinkles, they are particularly well suited to travel.
Date published: 2017-04-08
Rated 5 out of 5 by Cosby from Best Polo Columbia makes Normally wear a Large in shirts, bought an XL in this polo and it fits great. The polo is a great all around shirt. Highly recommend this polo, I wish Columbia would make them in the same colors as the PFG shirts though.
Date published: 2017-03-11
Rated 5 out of 5 by DapperedNY from Like a second skin Purchased 1 shirt originally never having any other experience with the Columbia brand .Have to say this garment far exceeded my expectations , fit quality and appel,since I have purchased several more items from Columbia
Date published: 2017-01-22
Rated 5 out of 5 by Barreli32 from Men's New Utilizer Polo This shirt looks good and is made of high quality materials with good craftsmanship, but the size indicated seems to be a little too small. The color options are decent, but a good value nonetheless.
Date published: 2016-11-27
Rated 5 out of 5 by Mooney324 from Great shirt! I am 6'2" and 185 poundsn with a athletic / dad-bod build- I ordered XL and it fits great. Definitely something that will be going into high rotation.
Date published: 2016-05-07
Rated 4 out of 5 by Bobby8411 from Fits well and feels great! This shirt is a cool color and it is breathable. I am a pretty skinny guy, so this medium fits me perfectly.
Date published: 2016-02-26
Rated 5 out of 5 by Sunny194728 from great! This is a great product and I would definitely recommend it!]
Date published: 2015-12-30
Rated 4 out of 5 by IslandBobby from Great Polo! Nice shirt! Great fit & feel. True to size and good quality. Bought the Graphite color...will order at least 3 more in different colors.
Date published: 2015-11-07
Rated 5 out of 5 by TexanTraveller from The perfect travel shirt? I bought 5 of these and went to SE Asia (Vietnam & Cambodia) for 2 weeks in August. Nothing short of air-conditioning is going to keep you from sweating, even when you're just standing there, but these shirts stayed comfortable all day. Even when wet, whether from sweat or water from a cooling towel around my neck, the shirts held their shape and didn't stick to my body the way pure cotton does. I like the mesh weave of this fabric better than the silky-smooth fabric of other "performance" brands and the polo style (i.e. collar) is appropriate for more activities other than exercise. The BEST feature, however, was that I could wash these shirts in the sink, hang them up on the shower rod or closet, and they'd be dry by morning and ready to go again. I brought a total of 10 shirts on vacation, but used these 5 over and over.
Date published: 2015-08-27
Rated 2 out of 5 by JasonsNickname from Too hot The cut and fit of these shirts is nice, and I'm a fan of the colors. Unfortunately, they're too hot to be tolerable. I feel like I'm wearing a jacket every time I step outside.
Date published: 2015-08-15
Rated 5 out of 5 by LTCHankster from Great For Golf Very happy with this shirt. Has enough room in the chest and shoulders to enable me to get a full swing with a golf club without the feeling of binding or tightness. These are my favorite golf shirts. Wish it was available in a dark hunter green.
Date published: 2015-08-09
Rated 5 out of 5 by Gregels from Polo Very light and breathable.
Date published: 2015-06-28
Rated 5 out of 5 by rfoot from Golf Shirt These shirts have excellent appearance and texture. The fit is perfect. The only thing that I would change is to increase the SPF rating to 50.
Date published: 2015-06-22
Rated 5 out of 5 by eculizar from Another great product from Columbia! I just recently purchased this shirt. It was very comfortable and cool. The only issue I had was when my dachshund climbed up my chest, the shirt piqued from his nails very easily. Other than that, great shirt!
Date published: 2015-06-18
Rated 1 out of 5 by Matt81 from Scratchy Neck The blue silver materials that band around the inseam of the neck are stiff and sharp. They scratch at the neck creating an uncomfortable experience. Not even an undershirt prevents this effect. I am quite satisfied with the shirt apart from this problem, but it is a big enough issue that I do not where this shirt whenever I have an alternative option.
Date published: 2015-06-14
Rated 5 out of 5 by 1Patriot1 from Great shirt Excellent quality. Very light and comfortable. Runs true to size.
Date published: 2015-04-30
Rated 1 out of 5 by CB75 from Nice, light but snags First off I have to say that I love the moisture wicking properties of this shirt. Working in South Forida, a moisture wicking shirt is a must and they got it right with this shirt. The major problem is with the construction and material used. Half way through the first day wearing my shirt, it was completely ruined with 4 huge snags right in the front of the shirt. U also noticed multiple small snags all over the shirt. I bought 2 of these shirts and have not worn the other one in fear the same will happen to it as well. I expected a higher quality product for the price.
Date published: 2015-04-21
Rated 4 out of 5 by crbishop3 from Very Comfortable Shirt runs a little small but still very comfortable and cool. Will go for the XL next purchase.
Date published: 2015-04-09
Rated 5 out of 5 by Ukintheusa from Great fit, low maintenance I own 3 different colors of this shirt. They are great. Low maintenance. They dont wrinkle. You can pull them out of the closet or drawer or suicase and throw it on. I can wear it 3 or so times before it needs washing as well which comes in handy when you travel. I am 5'10, 170lb and i wear the medium. Its not too tight but doesnt hang off baggy like. Slim fitting.
Date published: 2015-03-11
Rated 5 out of 5 by JasonTR from Great Thats good and breathable t-shirt. good or size fit for me
Date published: 2015-01-21
Rated 5 out of 5 by Jigsaw48 from Works for me Medium fit me as I expected - very comfortable. Spent time in Asia and it wicked well. My only concern is possible snagging due to weave although I have not had this happen. I have most colors and all have same look and feel.
Date published: 2014-12-28
Rated 2 out of 5 by Jago from Disappointed by website reviews Words cannot describe how disappointed I was not only by this shirt, but my entire order from Columbia. Of the five items (four shirts and one pair of shorts) I ordered specifically in time for a trip to Phoenix, only two were wearable. This shirt does not run "slightly small", it runs almost very small. I thought Columbia was selling to Outdoorsmen and athletes? Why are these shirts so small across the shoulders and chest?
Date published: 2014-07-11
Rated 4 out of 5 by slimduff from sleeves sleeves too short and too wide
Date published: 2014-02-11
Rated 5 out of 5 by Rator from Love this Shirt I am very particular when it comes to my polos; it has to fit properly and the buttons have to lay in a certain way. It seems as though not all mediums are created equal; most mediums are actually large. This shirt fits perfectly, meaning that the sleeve seam sits on the shoulder, and it is structured so it does not look like I am wearing a tent. It is also very comfortable and light. I like the shirt so much, that I plan on buying every color.
Date published: 2014-01-21
Rated 4 out of 5 by SteveP from Nice Polo so far Very nice fitting Polo shirt. Hopefully the material holds up well after a few washes, but so long as it does I will be ording more.
Date published: 2013-10-26
Rated 5 out of 5 by Jeffers52 from This product has an athletic fit. This is an excellent product that has an athletic fit. Most products of this nature always seem to run large; even for a men's small. This product is the perfect fit. It is neither too loose or too tight. The color is excellent. I have received many compliments on this item.
Date published: 2013-10-22
Rated 5 out of 5 by DMoe from Love this Shirt! Glad that I was able to get a pass to visit the ES and try on this shirt prior to ordering; I usually wear an XL in just about everything, sometimes a L depending on the brand. I noticed that the XL in this shirt runs small for my liking, So reluctantly I tried on the XXL and it was a great fit. Maybe someone in the factory got the sizing mixed up :) The shirt moves well, breathes well with a great feel and look. I ended up buying 2 in the store and 1 on-line.
Date published: 2013-10-01
Rated 1 out of 5 by Visitor from poor fit Poor Fit Very tight under armpits. Sleeves very short
Date published: 2013-09-26
Rated 5 out of 5 by Instructorj5 from Utilizer Polo I originally bought one of these in the store as a gift. I ended up going back and getting one for myself then ordered two more online. I really like the material and the weight of the fabric. Quality product.
Date published: 2013-09-25
Rated 5 out of 5 by Live2Fish from Great These shirts a comfy and breath. I wear them for casual dress at work and golfing. I love them!
Date published: 2013-09-24
Rated 5 out of 5 by Dani8 from You will love this shirt This was one of 4 shirts in my order. The other three were the Skiff III T-shirts and the fit was so different and had to be returned. Washes great without creases. Ideal for the traveller.
Date published: 2013-09-07
Rated 5 out of 5 by Zebra from Comfortable, wicking material. At age 59, I prefer to NOT wear a cotton t-shirt underneath my casual clothing. This shirt fits the bill, the wicking material absorbs moisture, and keeps me dry. I had skin cancer removed from my back in January, and am intrigued by the SPF attributes that Columbia integrates into its shirt design. Great colors, comfortable in warm weather. I am 6'0", 200 lb. Large fits me well.
Date published: 2013-08-05
Rated 5 out of 5 by Troutman from Great shirt Mountain color is great. Shirt very cool with good mobility. Will probably get more in other colors.
Date published: 2013-07-10
Rated 4 out of 5 by Burley355 from Good to Excellent but Columbia Logo Black I'm currently wearing the white Utilizer. I'm very impressed with the way it fits. One of the best shirts I've ever owned in that regard. Can wear it tucked or untucked just fine. My only gripe might be that Columbia's logo - a fine logo - is not inconspicuous. The white shirt appears to have a white or even gray logo, but it's actually black. It really sticks out. It's a good shirt for someone who doesn't mind advertising for Columbia. On the other hand, the quality is so good, I don't mind bragging.
Date published: 2013-07-05
Rated 4 out of 5 by CJ2013 from Cool shirt - snags easy Nice shirt, cool fabric but gets small snags easy
Date published: 2013-06-04
Rated 5 out of 5 by KeysCaptain from Great comfort and style These shirts are very comfortable and stylish enough for a dinner or night out.
Date published: 2013-06-03
Rated 5 out of 5 by gjesse from Love these shirts! These are great shirts, and I liked them so much I bought a few in different colors. This is a nice casual shirt that goes with jeans or khakis so it can be worn on casual Fridays, relaxing around the house, or out on the trail. Made from a super comfy fabric, that's sure to please. I hope Columbia continues to make this product line for a long time!
Date published: 2013-03-23
Rated 5 out of 5 by BigEd from versatile shirt This is a very versatile shirt. I can wear it to our grandson's sporting events and also when my wife and I go out to a casual dinner. I am 6' 3" and I like my shirts to fit a little lose. I find that a 2XL meets my needs. I would recomend this shirt in most of the colors avaliable. Big Ed
Date published: 2013-03-08
Rated 5 out of 5 by JasonA from Comfortable, lightweight shirt This line of shirts are extremely soft, comfortable, and quick-drying. The mesh-like pattern is prominent, but not so much as to take away from the style or quality of the shirt. The only negative of this line of shirts is that, due to the open weave of the material, small pulls will form relatively quickly but not so much as to render the shirt unwearable.
Date published: 2013-02-18
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Size & Fit Guide
Size Chart
Size & Fit Guide
Men's Body Measurement (Inches)
|Sleeve Length
|Sleeve Length
How to Measure
- 1. Neck: Measure at the base or thickest part of your neck.
- 2. Sleeves: Start at the center back of your neck and measure across the shoulder and down to the wrist. Round up to the next even number.
- 3. Chest/Bust: Measure at the fullest part of the chest, under the armpits and over the shoulder blades, keeping the tape measure firm and level.
- 4. Waist: Measure around the natural waistline, allowing the tape measure to sit comfortably.
- 5. Hips: Measure around the fullest part of your hips.
- 6. Inseam: Measure from the top of the interior leg to the bottom of the ankle.
Men's Fit Guide
Body contouring fit conforms to movement.
Body skimming fit with end-use mobility in mind.
Modern Classic / Regular
Universal, easy fit for comfortable range of motion.
Most generous fit for a full spectrum of movement.
How to Measure
How to Measure
- Fold up at dotted line.
Place against a wall or other 90 degree surface.
Place heel against fold/wall on flat level surface.
- Mark the end of the longest toe with a pen or pencil.
- Measure both feet and use the larger measurement.
- Read measurement and compare to size chart.
- When comparing on the size chart, determine the closest larger measurement and use that number to determine your shoe size for your country's shoe measurement standard.
Shipping & Returns
Shipping & Returns
Shipping Information
|Shipping Method
|Expected Delivery Time
3-7 Business Days
|2-Day Air
|2-3 Business Days
|1-2 Business Days
|Free Standard Shipping
|Available for Greater Rewards members. Learn more
Return Policy
Returns are accepted within 60 days of the purchase date online or in-store. Your apparel must be in original and unworn condition, with tags still attached. Footwear returns must also include the original box.
Still Have Questions
See our Customer Service section for more details. Visit Customer Service


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Classic "Visitation" Storyline Back In Print...
To insure your action figure collection, get in touch with Collectibles Insurance, the official insurance company of the Figures.com network. Say "Figures.com" to get 5% off your first term premiums.
To buy action figures, take a look at BigBadToyStore.com, PlasmaInfusion.com, Toynk.com, BriansToys.com, ToyWiz.com, and MonkeyDepot.com.
While it seems like there couldnt be more Doctor Who for all the fans out there to consume, BBC continues to crank out classic storylines alongside the new stuff thats on TV now. These great presentations are bringing the classic episodes to whole new generations of fans while offering the longtime viewers a chance to re-live the early days of British science fiction on TV. This months classic release features The Visitation with the Fifth Doctor (Peter Davison) from 1982.
The Visitation takes place in England (go figure) in the year 1666. While the main issue for the locals is the Great Plague, another much more insidious threat has latched onto the Earth... revealed to be Terileptils (alien fish-like people) and their android! Taking control of villagers and plotting even bigger shenanigans, the Terileptils use advanced enslavement and weapons technology as well as their artificial lifeform helper who frightens innocents as the Grim Reaper itself. Its up to the Doctor and his companions, Adric, Nyssa, and fan favorite Tegan, to stop the threat while maintaining the temporal security of the Earth.
Another solid example of a classic Who storyline with impressive effects on a shoestring budget, Visitation features all sorts of innovative tricks. Most of all here are the historical locations and set dressings, costumes, and of course the aliens. While they might not be as scary to a modern audience as they were in the early 80s, the Terileptils are pretty impressive monsters with detailed anatomy and interesting textures. The android is pretty fun too, both in his normal appearance and the Reaper disguise. A later classic, The Visitation is in full color. Its remastered (as far as these things go for older TV shows), and as a BBC Special Edition comes with a slew of extras. On deck is audio commentary (main actors and director), the Grim Tales documentary on filming locations, The Television Centre of the Universe behind the scenes of BBC Television Centre, Doctor Forever: The Apocalypse Element doc about Doctor Who audio programs, extra footage, more docu-features, production notes, PDF sales sheets, photo galleries, trailers, and more!
As with all of the classic releases, The Visitation is a great blast from the past for longtime fans and a piece of history for newer viewers. With all of its 80s British TV charm and cool aliens this is a solid classic storyline and one youll need in your collection. The Visitation is available now wherever DVDs are sold.
Review by Scott Rubin
Review Sample Courtesy of BBC Home Entertainment

subset: commoncrawl


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Motorcycles lined Kanawha Boulevard Saturday as people gathered at Haddad Riverfront Park for the third annual Capitol City Biker Bash. The event ran Thursday to Saturday, featuring live music, motorcycle games, food vendors, a motorcycle giveaway and more. For the second year, the bash helped raise money for HospiceCare.
The event has grown each year, said Larry Hayes, president of the Capitol City Biker Bash. There were about 10 extra vendors this year and motorcycle parking was extended.
"There are a lot of people that like to ride," Hayes said. "This is a great venue to ride to and if you go outside of the city it's good riding, curving roads for motorcycles."
Hayes said riders came from as far as Oklahoma but many out-of-state riders were from neighboring Pennsylvania and Ohio.
The rock band Saliva kicked off the event Thursday. Hayes said Friday night's weather didn't deter attendees from hearing the band Midnight Special jam.
"They stuck it out," Hayes said. "They were out here watching the band, dancing in the rain."
Azim Smith of the local biker club, Buffalo Soldiers, said the event is a good time and great fellowship among riders.
Smith said the club, which formed last July, has about 27 members. They ride to bring awareness to the sacrifices, hardships and racism African American soldiers endured during the American Civil War.
"The bash gives us a chance to welcome people to West Virginia and show what motorcycle clubs can do for the community," Smith said.
Last week the club helped clean the veterans cemetery in Institute.
"What it has also offered us, because we are kind of the new kids on the block, is the opportunity for us to come out and introduce ourselves to the community," said club president, Rodney Booker.
Joe Delph of Berkeley Springs participated in the event's biker games.
"It's all about participating and having fun," Delph said.
There are several games, including the slow ride and jousting.
Riders jousting each have a pool stick that they use to capture rings from atop cones as they ride by.
Eddie Gillespie of South Charleston took home the event's largest trophy and a $100 cash prize for his 1996 soft tail Harley Davidson motorcycle. He won the best of show.
"I have a lot of motorcycles," Gillespie said. "I thought I'd put one in the show this year."
Gillespie, along with his son, has been working on the soft tail since 2003, adding "different stuff" to the bike.
His favorite addition is the bike's super charger, which provides the bike with "lots of power."
Gillespie tries to do all the work on his 15 bikes himself.
"It's just a fun hobby," he said. "It keeps me out of trouble."
Hayes said the event used social media and worked with the Charleston Convention and Visitors Bureau.
"We just wanted to bring people into the city," Hayes said. "Year-to-year we hope to keep it growing."
Reach Caitlin Cook at email@example.com or 304-348-5113.


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Walmart, Target and Best Buy attracted record levels of customers to their websites during November, according to data released this week by the online measurement firm comScore. Retailers have come to expect a surge in traffic to their sites as the holiday approach and during November that proved to be the case and then some.
Walmart and Target both experienced a 44% increase in the number of unique visitors to their sites during November when compared with October. The surge pushed Walmart to the 20th spot on comScore's ranking of the top 50 U.S. online properties with a total of nearly 52 million unique visitors, while Target landed in the 27th position with nearly 40 million unique visitors. The number of visitors to Best Buy's website grew at an even faster 75% when comparing November to October, which gave the company 28 million unique visitors and a 41st ranking on the comScore top 50 list.
"As the holiday season kicked off in November, Americans were quick to take advantage of retailers' early promotions and saving in crossing a f few items off their shopping list," said Jeff Hackett, EVP comScore Media Metrix. "Cyber Monday, the Monday after Thanksgiving, came in as the heaviest online spending day on record in the U.S. which contributed a strong portion of traffic growth at retail and coupon sites."
While Walmart, Target and Best Buy advanced their position on comScore's top 50 list dominated by the likes of Yahoo, Google, Microsoft and Facebook, Amazon.com remains king of the retail hill. It attracted roughly 84 million unique visitors during November, placing it 10th on the top properties list.
The November figures are impressive when compared with October, but less so when compared with November 2009. In fact, unique visitor growth on a year-over-year basis has moderated somewhat, with Walmart, Target and Best Buy posting increases of 5%, 2% and 7%, respectively. Amazon.com's unique visitor growth increased 8% when compared with November 2009.


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"The art of the con" continues on DVD June 7...
For a great selection of film, comic and TV action figures and collectibles, check out BigBadToyStore.com, BriansToys.com, ToyWiz.com, PastGenerationToys.com, Urban-Collector.com, ToyDorks.com, MonkeyDepot.com, HobbyLinkJapan.com, and Sekaido.com.
To insure your action figure collection, get in touch with our sponsor Collectibles Insurance.
The third season of the action-drama LEVERAGE debuts on DVD June 7, 2011 from Paramount Home Entertainment, timed to coincide with the Season Four premiere on TNT June 26.
Oscar-winner Timothy Hutton (Ordinary People) stars as the leader of his elite team who act as modern day Robin Hoods by standing up for those who have been victimized by the rich and powerful. The show also stars Gina Bellman (Coupling), Christian Kane (Into The West), Beth Riesgraf (Alvin and the Chipmunks) and Aldis Hodge (Friday Night Lights).
The LEVERAGE: The Third Season four-disc set includes all 16 episodes with audio commentary on every episode, a gag reel, deleted scenes, a look inside the writers' room and multiple behind-the-scenes featurettes. The DVD will be available for the suggested retail price of $39.99 U.S.
LEVERAGE: The Third Season is presented in widescreen with Dolby Digital English 5.1 Surround and English closed captions. Every episode includes audio commentary and the DVD disc breakdown is as follows:
Disc One:
- The Jailhouse Job
- The Reunion Job
- The Inside Job
- The Scheherazade Job
Bonus Features:
o Deleted Scene from The Scheherazade Job
o Gag Reel
Disc Two:
- The Double-Blind Job
- The Studio Job
- The Gone-Fishin' Job
- The Boost Job
Bonus Features:
o Deleted Scenes from The Studio Job and The Gone-Fishin' Job
o On Set with Colton & Aboud: The New Writers of "Leverage"
Disc Three:
- The Three-Card Monte Job
- The Underground Job
- The Rashomon Job
- The King George Job
Bonus Features:
o "Leverage": What Does a Producer Do?
o Inside the "Leverage" Writers' Room
Disc Four:
- The Morning After Job
- The Ho, Ho, Ho Job
- The Big Bang Job
- The San Lorenzo Job
Bonus Features:
o Deleted Scene from The Big Bang Job
o Behind the Scenes featurette
Source: Edited Paramount PR


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I'VE BEEN down to Oxfordshire a number of times and never come away with a win – until this week.
They were missing a couple of batsmen but you can only beat what's in front of you and we did that quite convincingly.
We were keen to bat first but we didn't win the toss. But we made sure that we imposed ourselves on the game straight away by taking four or five early wickets.
Once we'd done that, we were able to keep nibbling away at their order and we thought that 228 was an average score on a wicket that had a lot more runs in it.
Jayden Levitt then came in and had a tremendous innings.
We ended the first day at 152-2 but we did have a bit of a wobble towards the end – we still ended with a lead of 150 runs but I felt we could have pushed on towards the 400 mark.
We produced another very good bowling performance on the second day and if we hadn't let them put together a couple of late partnerships, we could have had it all wrapped up with a day to spare.
But we still decimated them and it's a fantastic win.
The first innings performance wasn't too different to a few games last year – Cornwall at Corsham springs to mind – but we followed it up and pressed home the advantage this time.
We've got a quarter-final that the players are really looking forward too now and there have been some really difficult selection problems because everyone is playing so well.
Jayden Levitt came in for Ed Kilbee and has to open the batting this weekend after his 150-odd and Tom Morton comes back in after scoring a century against Wales at Warminster.
James Hayward has performed well but he hasn't even made the 12 and then we've got to think a sixth bowling option because Mike Coles isn't fit to bowl – that means that Henry Langford will probably be involved because he gives us that.
We'll be looking to impose ourselves on Norfolk and carry on expressing ourselves.


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<< Back To All Events
Redstone Winterfest
Start Date: 14-Feb-2014 | End Date: 18-Feb-2014
Location / Venue: Redstone, Colorado
State: Colorado
Zip: 81623
Support the American Alpine Club with their first Cragging Classic, part of this year's Redstone Winterfest. Held over Presiden'ts Day weekend Winterfest lets you enjoy ice and mixed climbing, free sleigh rides, free gear demos, clinics, ski tours, dinners, beer tasting and other events. Routes range from WI 2 to WI 5, with mixed lines from M7 to M11. To register in advance or for more information visit aspenexpeditions.com


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Compatible D-Link Unified Access Points include:
The DWS-4026 is D-Link's next generation Unified Wired/Wireless Gigabit Switch with an array of advanced features and 802.11n support. With the ability to manage up to 64 unified wireless access points by itself and up to 256 unified wireless access points in a switch cluster, the DWS-4026 is a full-featured and cost-effective mobility solution for mid-to large enterprises and service providers. Extremely versatile and flexible, the DWS-4026 can be deployed as a Wireless Controller in the core network or as a L2+ PoE Gigabit Switch at the edge depending on the requirement. By centralising WLAN configuration and management functions, DWS-4026 enables network administrators to have control, security, redundancy, and reliability needed to scale and manage their wireless networks easily and efficiently.
Adaptable Wireless
Most of the current Wireless LAN controllers' architecture requires wireless traffic to return to the controller for centralised processing, causing unnecessary traffic delay. The DWS-4026 offers network administrators additional options: depending on the wireless application, wireless traffic can either be tunneled back to the switch for better security control, or locally forwarded at the Access Point for optimal performance.
Comprehensive Wired/Wireless Security
The DWS-4026 offers a state-of-the-art Wireless Intrusion Detection System (WIDS), which empowers network administrators to detect rogue Access Points and rogue clients as well as anticipate wireless threats, preventing them from causing damage to the network. With WIDS, administrators can activate various threat detections and use RF scans to sweep the entire wireless network for any possible security breach in advance. Other wireless security features include WPA/WPA2 Enterprise, 802.11i, Captive Portal and MAC Authentication.
Seamless Mobility
Wireless clients can enjoy seamless and uninterrupted roaming from AP to AP managed by a DWS-4026 even if they are not in the same subnet. Because the DWS-4026 employs various mechanisms such as pre-authentication and key-caching, wireless users can freely "roam" the entire network without needing to re-authenticate. The additional Fast Roaming feature results in disruption-free, reliable wireless connectivity crucial for mobile applications such as Wi-Fi IP Phones and wireless PDAs. Furthermore, the DWS-4026 supports advanced "AP-AP Tunneling", which is used to support L3 roaming for wireless clients without forwarding any data traffic to the Unified Switch. This can help to significantly reduce network traffic and save bandwidth.
Voice-Optimised Quality of Service (QoS)
The DWS-4026 is specifically designed and optimised for Voice over Wireless traffic with features such as Auto-VoIP and Voice VLAN. The Auto-VoIP feature explicitly matches VoIP streams and provides them with a better class-of-service than ordinary traffic.
Network Resiliency
The DWS-4026 offers a "self-healing" network capability to increase the resiliency of the entire wireless network. To make up for a sudden RF signal vacuum created by any "dead" AP (AP with DC power failure, for example), the DWS-4026 automatically increases the transmit output power of the neighboring APs to expand the RF coverage, thereby "healing" the network. Also, to ensure continuous connection for current clients, the DWS-4026 performs load balancing across access points when network traffic reaches a certain threshold by forcing additional clients to associate with other access points.
Maximised Flexibility
In addition to all its wireless capabilities, the DWS-4026 also excels as an advanced L2+ PoE Switch. Complete with dynamic routing using RIPv1/v2, ACL security, multi-layer QoS, comprehensive VLAN support, IGMP/MLD Snooping as well as dual 10-Gigabit uplink support, the DWS-4026 truly enables network administrators to integrate their enterprise class wireless network with their wired infrastructure. Businesses contemplating upgrading their current wired or wireless network can deploy the DWS-4026 to take advantage of its dual-role flexibility.
Simplified Management
Multiple DWS-4026s can form a Switch Cluster, which enables network administrators to manage and configure all switches from one single "Cluster Master." In addition, the Switch Cluster also manages information of all the Access Points as well as their associated clients. This helps to significantly simplify management and reduce maintenance efforts as the network scales up.
Number of 10/100/1000BASE-TX ports
Number of Combo 1000Base-T/SFP ports
Number of open slots for optional 10 Gigabit module
RS-232 Console Port
PoE Support (Standard(s)/Number of Ports/Maximum Power Budget)
Switch Capacity
64-Byte Packet Forwarding Rate
Packet Buffer
Physical & Environmental
Power Input
Power Consumption (maximum)
Dimensions (W x D x H)
Operating Humidity
Software Feature
WLAN Management Capability
Access Control & Bandwidth Management
Managed AP
AP Management
WLAN Security
L2 Features
L3 Features
Quality of Service
ACL (Access Control List)
LAN Security
* To demonstrate fast roaming in a PC, a wireless NIC (Network Interface Card) needs to support the fast roaming feature
|File Size
|21/3/2016 1:03:00 μμ
|2/6/2014 6:20:00 πμ
D-Link Hellas
Μιχαλακοπούλου 38
11528, Αθήνα
Πωλήσεις: 213 0020352
(E-Mail: email@example.com)
Υποστήριξη: 213 0020353
(E-Mail: email@example.com)
Quickly access parts of the D-Link site.


text:
Anago Practitioner - Simple Hoodie
Product Color:
Product information
Hoodie Anago Practitioner - Simple is the all-time ultimate in comfort with its classic, relaxed fit. On the surface rich cotton with Anago Practitioner - Simple, inside flannel for the softest sensation. Medium to heavy weight ensuring warmth and makes it great, weather as a throw-over in rainy and windy weather or to protect against the snow and ice in the winter.
Additionally warms head and hands, featuring a roomy reinforced pocket. A big and wide hood ensures extra coziness. Stretched cuffs and hem assure a lasting shape. Anago Hoodie comes in 12 basic colors. Heather Grey: 80% Cotton/20% Polyester. Remaining Colors: 100% Cotton.
Sku: SC1BL0000024780909090029WH0000000


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Wednesday, January 10, 2007
Scenes from In-Person Registration
"You're wearing a suit, so I figure I can ask you a question."
"I'm a math major."
I look at his transcript.
"You got a D in pre-calc."
"Yeah, but I'm good at math. I just do badly on the tests."
"Calc has tests, too."
"I'm going to change the world."
"So can I get into (closed class)?"
"What's your major?"
"First it was chemistry, then criminal justice, then I switched to childhood education, then psychology."
"What do you want to do?"
"Blow less than the 40 thousand I blew at (other school)."
"I want to get into Comp II."
"Did you take Comp 1?"
"Yeah, at my last college."
"Do you have a transcript?"
"Like a report card."
"Oh. Don't you have it?"
We check online and print out an unofficial transcript.
"I don't see an English course."
"Uh, it's that one." (points)
"That says History."
"Yeah, but I wrote a lot of papers."
"It looks like you need to take Comp 1."
"I took that last semester!"
"Yes, but you failed it."
"But I took it!"
"I want a full-time schedule. Can we add some more?"
"Maybe. Do you have a job?"
"How many hours?"
"Full time, Monday to Friday."
"Oh. Any other obligations?"
"I have two kids."
"I'm thinking maybe we start you off part-time so you can get some momentum going. I'd rather you pass two classes than fail four."
And, not to be outdone, three from my department chairs:
"We found the tuba!"
"The kiln exploded."
"I lost an adjunct, but I got a replacement."
"I called her on her cell. She took the call as she was being wheeled into surgery."
"She'll be here Tuesday."
Great collection. I have to start writing these things down...
"I took Soc 14 last semester and passed it."
"We don't have a course called Soc 14."
"Well, you must, because I took it and passed it."
I've got to remember to take notes next time at registration, or even during in-office advising.
"I want to major in Cosmology, so I can learn to cut hair."
"I want to take Astrology."
"Do you mean Astronomy?"
Sure, you laugh when you hear it, until you realize they are the future leaders of the world, and then you find a corner in which to weep silently.
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… Why does email deliverability matter? According to the 2015 Email Data Quality Trends Report by Experian, 73% of marketers reported having issues with email deliverability. Return Path has reported that over 20% of legitimate email goes missing. Undoubtedly, businesses encounter problems with deliverability, and that negatively impacts the bottom…
… Over 85% of the world's email traffic is considered malicious, making it much more difficult for valid email marketers to get their messages to the inbox. In some cases, being blacklisted can cause up to 70+% (maybe more depending on where you send mail to) of your email to be rejected or placed in the Spam/Junk/Bulk folder. Over 20…


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Topic: General Information about CC- a reprint from previous message
Side effects of blood transfusions are fever and transfusion allergic reactions such as itching ,rash and shortness of breath ,which if occur, will be in the begining of the transfusion and generally will be managed by premedicated with Tylenol 605mg and Benadryl(antihistamine. 50mg) before transfusion.
CEA and CA19-9 are tumor markers ,along with ALK phosphatase, to MONITOR the progress of the chemo treatment. Doctors are looking for a TREND rather than single value ,together with the Cat Scan result to determine the course of treatment.
If you have advance cancer or cancer metastasized ,CEA is more likely to have higher value;a steadyily rising CEA value often is the first sign of tumor recurrence.
If you have CC in your liver(intrahaptic),you will most likely to have a much lower CA19-9 value than if you have the ductal CC in or near the main bile ducts .
CA19-9 is ordered for checking bile duct blockage and that is why after putting in a new stent the CA19-9 will be lower.
ALK phos is indicated for bile duct's health;ALT and AST are related to the liver's health.AFP is biomarker for liver cancer.
Cat Scan is for diagnosis purpose.(including initial diagnosis and follow up after resection or chemo treatment for CC. Both MRI and Cat Scan are used to look for structural changes(ie:the size ).PET scan is used to look for functional changes (activity)of the CC.
According to one study compared 20 intrahepatic patients images ,the extent of the tumor enhancement was similar with both MRI and CT methods,however the relationship of the tumor to the vessels and surrounding organs was more easily evaluated on CT scan as opposed to MRI.But for perihilar tumors CT also has limited sensitivity for extra regional nodal disease(ie metastases to the periaortic,pericaval or celiac artery lymph nodes.)---from uptodate .com.
I myself think that MRI with contrast is a good choice after initial CT with contrast when inconclusive reported in the early stages of CC development(ie: size of the cc<2cm.) to rule out recurrence.
PET Scan allows visualization of CC because of the high glucose uptake of the bile duct epithelium(the lining )-- the "Hot spots" will light up on the PET scan.
A PET scan therefore can help to tell if the bile duct obstruction is caused by a cancer or not.PET scan can also be useful in determining the cancer may have spread or return after treatment.
Some hospitals equipe with machine that is able to perform both A PET and CT scan at the same time(PET/CT scan) ;this allows the radiologist to compare areas of higher radioactivity(SUVmax) on the PET with the appearance of that area(the location) on the Cat scan. But according to the radiologist I talk to , A (PET/CT scan ) is not the SAME as if you take them SEPARATELY;(PET/CT scan is PET plus CT scan WITHOUT contrast). Remember Ct scan is for structural and PET is for functional( activity) visualization. That is why sometimes doctors order a PET scan on this 3 month checkup and on the next checkup, he/she orders a CAT Scan with contrast or MRI instead.
Additional info. from uptodate.com
MRI and CAT SCAN (CT) have similar resolution for liver lesions.
CT has been considered to be superior to MRI for evaluating extrahepatic organs and calcifications. MRI is more specific than CT for differentiating cavernous hemangiomas,diffuse hepatic steatosis and focal fatty infiltration.Also MRI should be reserved for the evaluation of lesions less than 2 cm,or lesions located adjacent to the heart or to major intrahepatic vessels.If you are allergic to the IV iodinated contrast agent used for CT,then MRI is the alternative because the contrast agent used is different than CT. and MRI is not involved radiation .
Having SUVmax value show up on the PET scan does not automatically means you will have cancer activity on that "hot spot" location. It is highly depending on the location such as the liver,the lung or the prostate; and the condition of your health(ie: infection or inflammation on that part of the body).
I hope the above info. helps.
God bless;.


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The Essick Air Whole House Digital Humidifier 4DTS-400 is an evaporative cool mist humidifier featuring a 13 gallon output for areas up to 2700 sq ft. In addition to its sleek new design, the Digital Touch Screen Control Panel automatically maintains your desired humidity level with digital accuracy. It has dual 2.75 gallon tanks that hold up to 5.5 gallons of water, and a nine speed variable fan for convenient and flexible operation. The 4DTS400 also features a high output evaporative wick which retains minerals and delivers clean and natural moisture into the air.
Features and Benefits:
Bonus! FREE sample of Humidifier Bacteria Treatment included!
Essick Air 4DTS-400 Product Manual


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For example, why should Rove and his GOP elites be allowed to pick the U.S. Senate nominee for my home state of Iowa? Congressman Steve King has served nearly every conservative cause that matters with distinction. If the conservative grassroots wants King as their nominee in next year's election, following Virginia's lead would almost assure that would happen. After all, who should determine who represents our values? Those in the grassroots that actually fight for them, even when it's not popular, or Rove and his cynical Beltway bunch that really don't have too many core worldview differences from Democrats?
Besides, I love hoisting foes with their own petard. We originally went to a primary system to avoid the smoke-filled rooms of conventions, so it would be deliciously ironic to revert back to conventions to take Boss Tweed-wannabes like Rove and the big money out of the process and restore virtue and principal.
It's time we in the grassroots that do most of the heavy lifting for this party stopped allowing the Republican Party establishment and the liberal media, who are virtually one in the same nowadays, to determine our standard-bearers. Yes, some of our preferred candidates recently (i.e. Todd Akin, Richard Mourdock, etc.) haven't been up to the task, but others (i.e. Cruz, Rand Paul, etc.) have been.


text:
HOLLINS residents have lost their fight to stop a "narrow" road being used to access a new housing site.
Morris Homes has asked Bury Council for permission to build 21 houses on The Hollins site, off Haweswater Crescent.
A total of 26 people formally objected: some were against the idea altogether, while others said they had no objection to the houses being built, as long as the proposal to allow vehicles to access the site from Haweswater Crescent was scrapped.
They said Haweswater Crescent is a narrow side street and had traffic-calming measures, which demonstrated there was already a problem with traffic — and the housing would make it worse.
Some also claimed the road gets icy in the winter, making driving treacherous.
Bury planning committee voted five to two in favour of granting permission without any amended conditions on the proposed access.
A report to the committee said: "The existing traffic-calming measures on Haweswater Crescent would be revised.
"There would be levels of visibility at the junction of the new access with Haweswater Crescent and the traffic section has no objections to the proposal."
When work is finished, the firm must spend £71,847 on improving recreation in the area.


text:
Upstream / Downstream
Explore pathways related to this product.
25% Off
the purchase of 3 or more products
To Purchase # 9376S
|20 µl (2 western blots)
|100 µl (10 western blots)
Find answers on our FAQs page.
PhosphoSitePlus® Resource
- Additional protein information
- Analytical tools
Phospho-PKCδ/θ (Ser643/676) Antibody #9376
Western blot analysis of extracts from U-937 cells, untreated or TPA-treated (0.2 µM), using Phospho-PKCdelta/theta (Ser643/676) Antibody.Learn more about how we get our images
Western blot analysis of Baculovirus expressed PKC isoforms alpha, beta, gamma, delta and epsilon, untreated or lambda protein phosphatase-treated, using Phospho-PKCdelta/theta (Ser643/676) Antibody (upper) or PKCalpha, -beta, -gamma, -delta and -epsilon antibodies (lower).Learn more about how we get our images
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-PKCdelta/theta (Ser643/676) Antibody.Learn more about how we get our images
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-PKCdelta/theta (Ser643/676). Antibody.Learn more about how we get our images
Gallery: Phospho-PKCδ/θ (Ser643/676) Antibody #9376
Western Blotting Protocol
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
A. Solutions and Reagents
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
- 20X Phosphate Buffered Saline (PBS): (#9808) To prepare 1 L 1X PBS: add 50 ml 20X PBS to 950 ml dH2O, mix.
- 10X Tris Buffered Saline (TBS): (#12498) To prepare 1 L 1X TBS: add 100 ml 10X to 900 ml dH2O, mix.
- 1X SDS Sample Buffer: Blue Loading Pack (#7722) or Red Loading Pack (#7723) Prepare fresh 3X reducing loading buffer by adding 1/10 volume 30X DTT to 1 volume of 3X SDS loading buffer. Dilute to 1X with dH2O.
- 10X Tris-Glycine SDS Running Buffer: (#4050) To prepare 1 L 1X running buffer: add 100 ml 10X running buffer to 900 ml dH2O, mix.
- 10X Tris-Glycine Transfer Buffer: (#12539) To prepare 1 L 1X Transfer Buffer: add 100 ml 10X Transfer Buffer to 200 ml methanol + 700 ml dH2O, mix.
- 10X Tris Buffered Saline with Tween® 20 (TBST): (#9997) To prepare 1 L 1X TBST: add 100 ml 10X TBST to 900 ml dH2O, mix.
- Nonfat Dry Milk: (#9999).
- Blocking Buffer: 1X TBST with 5% w/v nonfat dry milk; for 150 ml, add 7.5 g nonfat dry milk to 150 ml 1X TBST and mix well.
- Wash Buffer: (#9997) 1X TBST.
- Bovine Serum Albumin (BSA): (#9998).
- Primary Antibody Dilution Buffer: 1X TBST with 5% BSA; for 20 ml, add 1.0 g BSA to 20 ml 1X TBST and mix well.
- Biotinylated Protein Ladder Detection Pack: (#7727).
- Prestained Protein Marker, Broad Range (11-190 kDa): (#13953).
- Blotting Membrane and Paper: (#12369) This protocol has been optimized for nitrocellulose membranes. Pore size 0.2 µm is generally recommended.
- Secondary Antibody Conjugated to HRP: Anti-rabbit IgG, HRP-linked Antibody (#7074).
- Detection Reagent: SignalFire™ ECL Reagent (#6883).
B. Protein Blotting
A general protocol for sample preparation.
- Treat cells by adding fresh media containing regulator for desired time.
- Aspirate media from cultures; wash cells with 1X PBS; aspirate.
- Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate). Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Keep on ice.
- Sonicate for 10–15 sec to complete cell lysis and shear DNA (to reduce sample viscosity).
- Heat a 20 µl sample to 95–100°C for 5 min; cool on ice.
- Microcentrifuge for 5 min.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Loading of prestained molecular weight markers (#13953, 5 µl/lane) to verify electrotransfer and biotinylated protein ladder (#7727, 10 µl/lane) to determine molecular weights are recommended.
- Electrotransfer to nitrocellulose membrane (#12369).
C. Membrane Blocking and Antibody Incubations
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
I. Membrane Blocking
- (Optional) After transfer, wash nitrocellulose membrane with 25 ml TBS for 5 min at room temperature.
- Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
II. Primary Antibody Incubation
- Incubate membrane and primary antibody (at the appropriate dilution and diluent as recommended in the product datasheet) in 10 ml primary antibody dilution buffer with gentle agitation overnight at 4°C.
- Wash three times for 5 min each with 15 ml of TBST.
- Incubate membrane with Anti-rabbit IgG, HRP-linked Antibody (#7074 at 1:2000) and anti-biotin, HRP-linked Antibody (#7075 at 1:1000–1:3000) to detect biotinylated protein markers in 10 ml of blocking buffer with gentle agitation for 1 hr at room temperature.
- Wash three times for 5 min each with 15 ml of TBST.
- Proceed with detection (Section D).
D. Detection of Proteins
Directions for Use:
- Wash membrane-bound HRP (antibody conjugate) three times for 5 minutes in TBST.
- Prepare 1X SignalFire™ ECL Reagent (#6883) by diluting one part 2X Reagent A and one part 2X Reagent B (e.g. for 10 ml, add 5 ml Reagent A and 5 ml Reagent B). Mix well.
- Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Western Blot Reprobing Protocol
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
A. Solutions and Reagents
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
- Wash Buffer: Tris Buffered Saline with Tween® 20 (TBST-10X) (#9997)
- Stripping Buffer: To prepare 100 ml, mix 6.25 ml of 1M Tris-HCl pH 6.8, 10 ml of 20% SDS and 700 μl β-mercaptoethanol. Bring to 100 ml with deionized H20. Make buffer fresh just prior to use.
B. Protocol
- After film exposure, wash membrane four times for 5 min each in TBST. Best results are obtained if the membrane is not allowed to dry.
- Incubate membrane for 30 min at 50°C in stripping buffer (with slight agitation).
- Wash membrane six times for 5 min each in TBST.
- (Optional) To assure that the original signal is removed, wash membrane twice for 5 min each with 10 ml of TBST. Incubate membrane with LumiGLO® with gentle agitation for 1 min at room temperature. Drain membrane of excess developing solution. Do not let dry. Wrap in plastic wrap and expose to x-ray film.
- Wash membrane again four times for 5 min each in TBST.
- The membrane is now ready to reuse. Start detection at the "Membrane Blocking and Antibody Incubations" step in the Western Immunoblotting Protocol.
posted June 2005
revised October 2016
Immunohistochemistry (Paraffin)
A. Solutions and Reagents
- Xylene
- Ethanol, anhydrous denatured, histological grade (100% and 95%)
- Deionized water (dH2O)
- Hematoxylin (optional)
- Wash Buffer:
- 1X Citrate Unmasking Solution: To prepare 250 mL of 1X citrate unmasking solution, dilute 25 ml of SignalStain® Citrate Unmasking Solution (10X) (#14746) with 225 mL of dH2O.
- 3% Hydrogen Peroxide: To prepare, add 10 ml 30% H2O2 to 90 ml dH2O.
- Blocking Solution: TBST/5% Normal Goat Serum or 1X Animal-Free Blocking Solution.
- Detection System: VECTASTAIN® Elite ABC, including biotinylated secondary antibody (Vector Laboratories).
- Substrate: Vector® NovaRED™ (Vector Laboratories).
- Hematoxylin: Hematoxylin (#14166).
- Mounting Medium: SignalStain® Mounting Medium (#14177).
B. Deparaffinization/Rehydration
NOTE: Do not allow slides to dry at any time during this procedure.
- Deparaffinize/hydrate sections:
- Incubate sections in three washes of xylene for 5 minutes each.
- Incubate sections in two washes of 100% ethanol for 10 minutes each.
- Incubate sections in two washes of 95% ethanol for 10 minutes each.
- Wash sections twice in dH2O for 5 minutes each.
C. Antigen Unmasking
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
D. Staining
- Wash sections in dH2O three times for 5 minutes each.
- Incubate sections in 3% hydrogen peroxide for 10 minutes.
- Wash sections in dH2O twice for 5 minutes each.
- Wash sections in wash buffer for 5 minutes.
- Block each section with 100-400 µl of preferred blocking solution for 1 hour at room temperature.
- Remove blocking solution and add 100-400 µl primary antibody diluted in recommended antibody diluent to each section. Incubate overnight at 4°C.
- Prepare ABC solution per manufacturer's recommendations.
- Remove primary antibody and wash section three times with wash buffer for 5 minutes each.
- Add 100-400 µl biotinylated secondary antibody, diluted in TBST per manufacturer's recommendation, to each section. Incubate 30 minutes at room temperature.
- Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
- Cover sections with 100-400 µl pre-mixed ABC solution as needed and incubate in a humidified chamber for 30 min at room temperature.
- Wash section three times with wash buffer for 5 min each.
- Prepare Vector® NovaRED™ per manufacturer's recommendations.
- Apply 100-400 µl substrate to each section and monitor closely. 1-10 minutes generally provides an acceptable staining intensity.
- If desired, counterstain sections with hematoxylin (#14166).
- Wash sections in dH2O two times for 5 minutes each.
- Dehydrate sections:
- Incubate sections in 95% ethanol two times for 10 seconds each.
- Repeat in 100% ethanol, incubating sections two times for 10 seconds each.
- Repeat in xylene, incubating sections two times for 10 seconds each.
- Mount sections with coverslips and mounting medium (#14177).
posted June 2005
revised March 2016
|Western Blotting
|Immunohistochemistry (Paraffin)
Phospho-PKCdelta/theta (Ser643/676) Antibody detects endogenous levels of PKCdelta only when phosphorylated at serine 643, and PKCtheta only when phosphorylated at serine 676. This antibody does not cross-react with the phosphorylated PKC isoforms alpha, beta, gamma or epsilon.Species Reactivity: Human, Mouse, Rat, Monkey, Xenopus
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser643 of rat PKCdelta. Antibodies are purified by protein A and peptide affinity chromatography.
Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction (1,2). PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters (3-5). Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs in vivo at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660 (2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCμ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization (6). PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity (7).
1. Nishizuka, Y. (1984) Nature 308, 693-8.
2. Keranen, L.M. et al. (1995) Curr Biol 5, 1394-403.
3. Mellor, H. and Parker, P.J. (1998) Biochem J 332 ( Pt 2), 281-92.
4. Ron, D. and Kazanietz, M.G. (1999) FASEB J 13, 1658-76.
5. Moscat, J. and Diaz-Meco, M.T. (2000) EMBO Rep 1, 399-403.
6. Baron, C.L. and Malhotra, V. (2002) Science 295, 325-8.
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


text:
Hand-Tufted, Supple wool.
The Volare rug collection is an assortment of rugs that will satisfy every aspect of modern interior design. Affordably hand-tufted from soft, supple wool, each rug features an extra dense construction that adds texture and dimension. Hand-tufted. 100% Wool.
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Category: baby name Peregrine
This year our menu of Thanksgiving names draws on a variety of sources—from the Mayflower passenger list to prominent Pilgrims, to a harvest deity to the bounty of the Turkey Day table. Enjoy it— with it comes our best wishes for a very happy holiday to all our dear berries.
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text:
Week after week, the mailman climbed the steep hill of Shenango Street to the house with the busted porch steps. "Dear Miss Rouzzo," the letters began, or "Dear Tabitha Rouzzo." The college catalogues barely fit in the mailbox. They stuck out like gift-wrapped presents against white aluminum siding gone dingy from decades of wear. On the porch were three new Linen Breeze decorative candles -- a nice try, thought the actual Tabitha Rouzzo, who came walking up the hill every afternoon with her mind on the mailbox.
The 11th-grader seldom brought anyone home, and when she did she would sort of draw in a breath and say, "Well, here it is."
Her Victoria's Secret bag was crammed with track clothes and school papers. At 17, with dark hair and dark eyes, she was a version of the actress Anne Hathaway if Anne Hathaway had stars tattooed on her hip, chipped blue nail polish and lived two blocks from the projects.
Tabi shared the rental house with her mother and sometimes her mother's boyfriend. Her four older siblings were grown. None of them had graduated from high school. They wore headsets and hairnets to jobs that were so futureless that getting pregnant at 20 seemed an enriching diversion. Born too late to witness the blue-collar stability that had once been possible, they occupied the bottom of the U.S. economy.
"I'm running from everything they are," she said.
The question was whether Tabi could outrun the odds against her.
She knew that colleges sent out millions of letters to 11th-graders who took the Princeton Review prep course. The whole Dear Tabitha campaign was about as personal as fliers from Tire Express. But nearing the end of her junior year of high school, without a single item of value to secure her future -- not even a $50 U.S. savings bond from a departed relative -- the mail was all she had.
So she sweated it out the old-fashioned way, joining Spanish Club, Chess Club, Bible Club, Art Club and the track team, where she may have been the worst pole-vaulter in the Pennsylvania-Ohio border region. On Wednesday nights, she was at church waving her praise hands in the air, and on Friday night, it was a school production of "No, No, Nanette."
With no working vehicle at home, she had to walk most places. You could see her hoofing across the industrial landscape, her pink bag slung over her shoulder.
Tabi kept the college mail upstairs in her bedroom. She wrote back to 22 schools that offered biochemistry programs. Her goal was to be a forensic scientist in North Carolina. "It seems nice," Tabi said, though she had never been. She had never flown on an airplane. Her laptop was a secondhand PC she bought from a guy for $60. Her bedroom window overlooked a field strewn with Filet-O-Fish wrappers and Keystone Ice empties and, lower in the valley, the stacks at Ellwood Quality Steels chugging smoke.
Long before the recession, New Castle was a place of vanishing opportunity. It was 50 miles from Pittsburgh but felt farther, and while Steelers banners hung from awnings, the hard hat was a remnant of the past. Retail and food service jobs now outnumbered manufacturing jobs in the county. The top three employers were the hospital, state government and Liberty Mutual insurance company. Number seven was Wal-Mart, where Tabi's older brother worked in dairy until he was fired for stealing an energy drink.
Tabi heard stories about the olden days. She came from welders and ceramic production workers. But, to Tabi, the sprawling Shenango China factory where her grandfather and great-grandfather worked was just a boarded-up place on the way to Wal-Mart.
Her New Castle was the one that existed now: white, working class, with poverty that had deepened into the second and third generations. Nearly three-fourths of the students in Tabi's school qualified for free or reduced-price lunches, and one-third of New Castle families with children younger than 18 had incomes beneath the poverty level.
During the 2012 election, the campaigns of President Obama and Mitt Romney visited Pennsylvania a combined 38 times. With Ohio next door, the candidates and their wives barnstormed the region like few other places, focused almost entirely on the economy and strengthening the middle class. After the election, New Castle was still a hard town to be young and poor in.
They had $50 prepaid phones and $5 Day-Glo earbuds with the Chinese innards spilling out. They went to Township Tan for the 15-minute prom special. But the backwash of America's affluence was a dim substitute for the promise of the middle class, which had moved farther from their reach. The decline in economic mobility has made the bottom more difficult to climb up from.
Unlike her counterparts in higher ranges, prepped for ascension, Tabi Rouzzo had only herself.
At 13, she started working in a deer slaughterhouse. Her friend Gloria told her about it, and Gloria's mom drove them out there. They were greeted by a cold room with kerosene heaters. For $10 an hour, Tabi was to stand at a table cutting butterfly filets.
With a bloody knife in her hand and a circular saw whining behind her, labor laws being violated by the minute, Tabi decided on the spot that work offered freedom. She went back the next two winters, through 10th grade. Off-season, she cleaned rental properties, clerked in a mini-mart and baled hay at a farm.
In 11th grade, Tabi needed a job she could walk to and found Splitstone Entertainment, a storefront that sold used electronics, along with a selection of stun guns, nunchucks, ninja throwing stars and factory-boxed Star Wars collectibles. People brought in their Xboxes and PlayStations to unload, and Tabi cleaned them for resale.
"The controllers are real greasy," she said one Saturday afternoon, pulling back her hair for the task. She was not complaining. Even describing the slaughterhouse, she sounded like a butcher and not a squee girl. "I'm a grown man," she joked. It was somehow true. She had not a line on her porcelain face but a weariness was already in her.
After work that night, she met Gloria at Sheetz for dinner. Gloria was working midnights at the Subway inside the Pilot gas station for $8.60 an hour and was no longer in school.
"This town is dragging everyone down," Tabi said a few days later.
The mailbox at home remained a repository of hope. Tabi's mother brought the mail in every day. "She's got colleges all the way from Texas wanting her," said Patricia Edmonds, bragging about her daughter.
Tabi didn't spend much energy correcting the record. School, and her future, had always been hers to figure out.
Her mother had five kids and no husband at age 23. Tabi, the last born, was a welfare and WIC baby who grew up with evictions and lights getting cut off. Her 39-year-old mother remembers it differently.
"I tried to give them everything," Patricia said. "You wouldn't find one of my kids without a matching bed set."
Monthly income for Tabi and her mother at the house on Shenango Street was an $824 check from Social Security, food stamps and survivor's benefits for Tabi from the death of her father, a welder who died of an overdose. Patricia spent a lot of time on Facebook, posting shout-outs to her four grown children, "I'm cookin' sauce, you comin' home or what?" She listed herself as a "Stay at home mom" with a qualifier -- "QueenBitch."
Tabi thought her mother should get a job. "I don't ever want to sit on my butt, waiting on hand and foot for someone to help me," Tabi said.
It was her greatest motivation. The college mail reminded her how badly she wanted to escape her mother's destiny. And yet the glossy pictures of emerald campuses revealed how far away that green world was.
Tabi's alarm for school went off at 5:45 a.m. It sounded like a firehouse bell, as if to stress the urgency of the moment. Tabi used to share the room with her older sister. One morning, Tabi's sister decided not to get up, and that was that for high school. Tabi pulled herself from bed.
The crowded halls of New Castle Junior/Senior High School enveloped her. "Yo, Tabs," a teammate from track called. Tabi wore khakis and ballet flats. The hard protective shell Tabi wore at home was gone.
Despite her aspirations, Tabi was not pushing herself at school. She rarely brought work home. Some of her teachers used class time to let students complete their assignments. If Tabi had extra homework, she blew through it at lunch. Even so, she maintained a 3.0 GPA while taking honors courses.
Four and five decades ago, when New Castle High was full of factory workers' kids, the school taught Chinese, Latin, German, Spanish, French and Italian. Now it was Spanish, French and Italian. As students became poorer, standards dropped lower. Tabi's junior year, the average SAT score was almost 200 points below the national average. To boost scores, the school has made the Princeton Review college prep course free for all sophomores during class hours. Private tutors are luxuries of a different solar system.
Tabi planned to take the SAT before the school year ended. She kept reminding herself to sign up.
She worked other angles. The annual science competition was coming up. In the ninth and 10th grade, Tabi made it to the final round, and she was hoping for a repeat performance and a trip to Penn State.
"The main campus," Tabi said, as if speaking confidentially. "Maybe someone will see my project and help me get a scholarship."
What would have been nice was to be her friend Matt. He had an iPhone and two parents. They recently took Matt to visit Robert Morris University, a small liberal arts school outside Pittsburgh, and invited Tabi to come along.
A week later, in her school cafeteria, she was still talking about the trip. The campus tour was beautiful, and afterward, they met with an admissions counselor who estimated the cost of one year was $34,000. Tabi jumped in to warn Matt.
"I said, 'Matt, you're going to be paying off the loan for the rest of your life!' " she recounted at lunch. "His dad said, 'I think I got it covered.' "
There was more. On the way back to New Castle, they stopped for dinner at Olive Garden. Tabi couldn't help noticing the bill. "It was like $70!" Tabi said. "And it was no sweat off their back."
In the cafeteria, she went back to her free lunch and packed up for pre-calculus.
She needed to stop by the guidance office. At New Castle High, the office was the make-or-break room. It's where college-bound seniors stopped for applications -- for loans, waivers, scholarships and grants. Mrs. Gibson, the senior guidance counselor, helped them fill out the paperwork. She also arranged etiquette dinners that taught proper grooming, eating and the do's and don'ts of dressing professionally.
But decorating the ceiling of the guidance counseling office was her most inspired idea.
It was a patchwork of college T-shirts. They were stapled across the entire ceiling. Each was autographed by a New Castle High grad who had gone on to glory: Slippery Rock University, Youngstown State University, Robert Morris University, Butler Community College, St. Vincent's College, Clarion University, Penn State and Pitt. The parachute hovered like a subliminal cloud: You can do it.
The deadline to sign up for the SAT came and went. Distracted, Tabi forgot.
Every Wednesday night, Tabi got a break from the hand-over-hand climbing that consumed her life.
She usually sat in the same spot: the front row, closest to the preacher. When Pastor Shawn told Tabi she would look back in 20 years and be blown away by all the things God had done for her, she hoped he was right.
But on one night, the chairs were cleared out. An inspirational thrash metal band was performing live at First Assembly of God. Half the teenagers in New Castle seemed to be going, Jesus-loving or not.
For years, Tabi rode the church bus that swept through New Castle's threadbare neighborhoods picking up poor kids. On the night of the concert, Tabi got an upgrade. Her friend Miranda gave her a ride. Traveling by private coach was the way to go.
Not a single space was left in the parking lot as the arrivals poured in -- Tabi by car and the poor kids by bus. They tumbled down the stairs in a cloud of Sexiest Fantasies Body Spray. One girl was holding her MP3 player in the air like a transistor, the tiny rattling of Mindless Behavior's "Valentine's Girl" piping out through the 1-by-1-inch speaker.
Inside the church, God's abundance overflowed. Pastor Shawn had ordered enough pizzas and nachos to feed the Rust Belt. Shawn Galla, the 26-year-old youth pastor, had convinced church elders that a night of metal music and free prizes was more likely to bring in New Castle's teenagers than praise music and juice boxes.
Having grown up in working-class Pittsburgh with a single mother, Pastor Shawn thought he knew his audience when he took the job in New Castle in 2008, until he launched a fundraising drive for his kids and found their parents selling the Auntie Anne's pretzels for cash for themselves.
Tabi had inched her way to the front of the crowd when the lights went down and the screaming started. Pastor Shawn was on stage ready to start flinging CDs and McDonald's gift cards into the crowd.
"We're giving away free stuff!" he yelled. "EVEN JESUS!"
The band Icon For Hire was pierced and mohawked. "WHAT'S UP, NEW CASTLE!" the lead singer shouted, and the head banging commenced. The evening's motivational speaker, Seth Franco, a former Harlem Globetrotter, told his story of injury and comeback and invited anyone to raise their hands and come forward if they wanted to accept Jesus Christ.
"There's more to life than nothing," Seth said, as the electric keyboard softly lulled and the lights dimmed. "There's more to life than this town."
Words to Tabi's ears. She was not exceptionally pious and she had made her share of transgressions, but she always felt better at this moment when she closed her eyes and let go. The kids from the bus had their heads bowed, too. Some were wiping away tears, a few were sobbing, their shoulders heaving in the darkness of the church.
Then the lights blasted back on and Pastor Shawn was onstage, holding something small in his hand.
The silver sporty coupe arriving at Tabi's one Saturday night was so polished and punctual that it made the sagging house sag a little more. The Dodge Stratus idled at the curb. Tabi came down the busted porch steps in a skirt.
In Deric Lewis she had a boyfriend with the right mix of qualities. "He has goals," Tabi said. "He's kinda smart. He works. He's always there five minutes early."
But he was also a source of tension in the house and had stopped going inside. Tabi's mother said Deric was a snob and was turning Tabi against her family. Tabi said that Deric was the best thing that ever happened to her. Opening the car door, she left her mother and "Storage Wars" behind.
Deric was 19 and smelled of soap. He worked full time at Castle Cheese, where he wore a hairnet in 100-degree heat reaching into milky buckets of mozzarella for $9.65 an hour. His dad was a scrap-metal worker. Determined to have an office job someday, Deric was a full-time student at the community college.
He and Tabi were headed for the outlet mall in Grove City, 30 miles away, to see if Deric could use a $20-off coupon he had at Aeropostale. Tabi leaned in close as he drove, until he yawned, and she punched him in the arm.
"Hey!" he said, laughing. He reminded her of his 6 a.m. shift that day. Tabi pointed out that she had also worked eight hours that day.
They were the oldest teenagers in America.
All around them in the rural dark, energy companies were buying up land for natural gas exploration. Deric heard in class that Shell Oil was building a $3 billion refinery site in Beaver County. There were millwright jobs across the border in Youngstown. Deric wondered if he was doing the right thing pursuing a business degree, which would take three more years of killing himself at the cheese plant.
Tabi thought school was the answer, and they should stick to the plan.
"We're lucky; we both work," she said, as they arrived at the outlet mall. "We have the advantage that others don't."
They held hands as they walked to Aeropostale. Deric didn't find anything. He folded the coupon and put it back in his wallet. Tabi took an armful of clothes into a dressing room. It was nice to try on new things.
"How'd those work out for you?" a saleswoman asked. Aeropostale would not be getting a dime of Tabi's money. The $124 in her purse -- she was a fanatic about counting her cash -- was going toward a trip to Chicago with the Spanish Club. She returned every item.
Back in New Castle, they drove around, killing time. High on a hill, the lights of the city blinked below, and in the pockets of darkness were the abandoned mausoleums of industry.
"They say money doesn't buy happiness, but I would love to be crying in my Porsche," Deric said.
Tabi's phone flashed with a text. Keys in the mailbox.
"Well, mom's going out," Tabi said, sounding both annoyed and apprehensive.
Later that night, Deric brought Tabi home to her dark patch of Shenango Street. She was almost to her front door when she heard her name being called. The woman was vaguely familiar, a neighbor, someone her mother maybe knew. She asked to borrow Tabi's phone. Then the woman asked for a glass of water. Tabi put her stuff down and went to the kitchen.
When she came back with the water, the woman was gone and so was Tabi's purse.
With crystal force, the stolen purse exposed the tension between Tabi and her mother, proven out in the weeks that followed.
Tabi partly blamed herself for responding to someone on the street at 1 in the morning. But she also blamed her mother for living in a neighborhood where people needed money at 1 in the morning.
For years, Tabi hid her cash in small stacks around her bedroom. Tabi was more flush than her mother. If her mom asked for a loan, Tabi charged 20 percent interest. Once, when her mom ran out of food stamps, Tabi, as if to impart a lesson, went out to eat.
Patricia Edmonds felt her daughter's judgment. Tabi was different from her other four. Patricia marveled at her as a spectator would, watching something rare and unexpected.
"She wants so much for herself," Patricia said.
Patricia hung Tabi's awards on the living room wall. The sprawling constellation gave Patricia a tangible sense of accomplishment.
In her face and spirit were traces of the cheerleader who got pregnant in the eighth grade. Patricia's father was a welder and her mother a nurse's aide. The love of her life was a dark-haired welder named Frankie Rouzzo, Tabi's father. They had two daughters and Tabi on the way when they split. He died when Tabi was 10, and since then Patricia had maintained a fragile livelihood on the survivor's benefits for her three daughters.
She tried pleasing her vegetarian daughter, buying Tabi her favorite chocolate soy milk and making special trips to Wal-Mart for the bags of lettuce Tabi liked. "I made her Taco Bell Grande with tofu meat," Patricia said.
But Tabi had withdrawn. She came home from track practice, poured some lettuce on a plate, doused it in ranch and took her dinner upstairs.
The explosion happened on a Saturday night. Patricia was bigger, badder and louder than Tabi. But Tabi had resentment that went back years.
She said Deric hadn't brainwashed her against her family; the feelings were entirely her own. There was a difference between bad luck and bad choices, Tabi said, and she had grown up captive of her mother's choices.
"You think you're better then me, don't you?" Patricia yelled. "I had five kids!"
"Mom," Tabi yelled back, "you quit school. Does it dawn on you after your first [child] not to have a second one?"
It was a lethal blow, as only a teenage girl could deliver. Patricia got pregnant in the eighth grade, the same age Tabi was when she started at the slaughterhouse.
Patricia grounded Tabi for a month. She confiscated her phone -- which Deric had paid for -- and banned all activities except school and work.
One day after school, Tabi went to see her Uncle Bill about moving in with him. He worked at the jail and was a steady presence. When Tabi was 10, she went to stay with him when her mom was in a period of chaos. He took her to violin lessons.
Standing on the sidewalk at Uncle Bill's, the sun beat down. Tabi and her uncle and his wife were quiet.
"What are you gonna do?" Bill said, still in his jail uniform.
"It's up to you, Tab," Sybil said.
Tabi left their house on foot. She took the broken sidewalk that led her downtown. The beige mannequins and the Coney Island, the old motor lodge and legal disability clinic, Tabi hardly looked up.
"If I move out, my mom would lose the check, lose everything," Tabi said, weighed down by the decision.
When she reached home, it was different. Pine scent wafted. Folded stacks of laundry sat on the couch. The cleaning spree went on all week. Patricia went room to room, carrying out bags to the curb and posting her progress on Facebook. She was serious about taking a course in emergency medical technician training.
Patricia declared she was turning it around. Tabi had heard it before. But this time, her mother made a promise and extracted one from Tabi -- she had to break up with Deric.
Wanting to believe, Tabi agreed.
On a Saturday morning in June, Tabi walked to school to take the SAT. She had remembered to sign up for this one. Five interminable hours later, it was over, and Tabi went to work.
Summer without Deric was empty. There were grimy Xboxes to clean at Splitstone and swimming at the rock quarry. On Friday nights, Tabi and a friend hung out at a convenience store where a handwritten sign on the beverage station said, in friendly curlicue, "Smoothies, Slushies and Fountain pops cannot be bought with food stamps!"
Tabi got her first plane ride -- a church mission to Guatemala.
When school started in the fall, senior year felt different. An hourglass had been turned and the sand was falling. The college buzz greeted Tabi in the hallways, and it gave her the feeling that she was somehow already behind.
"Everybody's asking, 'Where are you going?' " Tabi said. "That worries me I don't have it figured out."
Applications to fill out, deadlines to meet -- it all hovered. Her SAT results were not what she hoped. They were above the average score at New Castle but well shy of the national median.
But Tabi, a master of contingency, already had a Plan B. On top of school, she started night classes to get certified as an EMT. True to her promise, her mother enrolled, too, and they sat side by side, sharing Tabi's textbook.
Patricia, who had not been in a classroom since eighth grade, spent afternoons at the public library. She and Tabi left Shenango Street for a new rental house, funded with help from Patricia's boyfriend.
Buried in school, work and EMT training, Tabi began to recalibrate.
The Navy recruiter was in the cafeteria at school when a 17-year-old girl approached. She was ready to sign her name -- Tabitha Rouzzo. She didn't want to hear a pitch. Tabi had learned enough online about a reservist's pay and travel. College could wait. When it did start, tuition would be on the Navy and Tabi would be gone from New Castle.


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Tuesday, January 18, 2011
Baking With Dorie: Apple Coconut Family Cake
7 comments:
- dorie said...
I love the way you turned the cake into muffins/cupcakes. They're very beautiful.
- 18 January 2011 at 16:33
- ♥Sugar♥Plum♥Fairy♥ said...
I lovee minis too , way way muc!
I think i ended adding less apple too , coz i used the rest after all the cutting and shaping i did, lol!
Ur cakes are pretty and love the glaze too!
Ur rite , i loved the sound of family cake too:-))))
Thannxxx a ton for baking my choice with me and giving me a chance too:-)
Lookign forward to the muffins next and we arent baking in feb ,is it?
- 18 January 2011 at 23:16
- Sage Trifle said...
I also love the term, "Family Cake". I love baking with apples and my husband loves coconut, so I'm sure these would be a hit in our family.
I especially like the little cake decorated by your daughter.
- 19 January 2011 at 05:30
- Beth said...
These look delicious! Any time I can cut a recipe in half, I feel virtuous. Apple flavored anything is awesome!
- 19 January 2011 at 19:34
- rebecca said...
now i'm wishing i put the glaze on. it's the perfect finishing touch. they look delicious.
- 21 January 2011 at 02:05
- laurie said...
I love the cupcake version. So cute!
I thought this was a very good cake..flavor... texture..all perfect!
- 21 January 2011 at 18:54
- tina_bakes said...
they are gorgeous!! sorry for being late on commenting - I had come here much earlier via mobile and did not manage to leave a comment. I love the cake - apart from the slight mush - and your photos are tempting me to try them again as cupcakes!! looking forward to your take on coffee break muffins!!
- 22 January 2011 at 18:09


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Peek into the Next 50 Years with IEEE Spectrum
Fanny Littmarck June 20, 2014
This month, IEEE sent out the 50th anniversary issue of their magazine IEEE Spectrum. This particular issue offers an inspiring and hopeful special report on what the next 50 years will bring in terms of technology advancements.Read More
Jennifer Segui June 19, 2014
It's likely that you've heard or read about many of the exciting discoveries in particle physics research at Fermilab. Powerful particle accelerators, including the Booster synchrotron with its unique ferrite-tuned RF cavities, consistently bring the lab to the forefront of discovery. Upgrading the 40-year-old Booster RF cavities will enable them to produce and sustain particle beams at even higher intensities… but will they overheat? Learn how the engineers at Fermilab address this important design challenge.Read More
Lexi Carver June 18, 2014
Cardiovascular disease is a condition where the arteries in the heart are blocked by plaque. Narrowed arteries can restrict blood flow and cause chest pain and shortness of breath. Bare metal stents can be used to resolve the problem, but excessive tissue can grow over them and narrow the artery again (a process called restenosis). Engineers at Boston Scientific are using simulation to understand the release mechanisms in drug-eluting stents, which can be used to prevent this excess cell growth.Read More
Mark Fowler June 17, 2014
When you lose power at home, you may use a shaker flashlight to navigate about your house. This type of flashlight relies on voltage produced by electromagnetic induction in order to be powered. How much voltage can one of these flashlights produce, do you think? Here, we find out through computation, using the AC/DC Module.Read More
Amelia Halliday June 16, 2014
If you are searching for a tutorial on how to model a miniaturized 3D electromechanics problem, then look no further. We have just published an updated version of our video tutorial on how to simulate a capacitive pressure sensor. COMSOL Multiphysics version 4.4 and the MEMS Module are used to simulate the electrostatic, structural, and thermal physics that occur.Read More
Fanny Littmarck June 13, 2014
Some chemical applications call for identification and quantification of the components in a chemical mixture. High performance liquid chromatography (HPLC) is a versatile separation technology for chemical species. To learn more about the separation process, we can model it with simulation software.Read More
Ed Fontes June 12, 2014
The Beckham and Maradona curl obtained with the inside of the soccer cleat (football boot), and the curl by Eder, Nelinho, and Roberto Carlos with the outside of the cleat, is due to the Magnus effect. The effect is named after the scientist who first observed it in a laboratory in the 1850s. The Magnus effect explains the side-force on a sphere that is both rotating and moving forward. We'll use it to analyze the FIFA World Cup™ match ball.Read More
Laura Bowen June 11, 2014
With all of the other components of an automobile to consider, it is easy for drivers to forget to routinely check tire pressure. Thankfully, companies are actually beginning to assemble most of their newer vehicles with built-in tire pressure monitoring sensors. These devices are placed at the bottom of the tire hub and measure air pressure automatically — all while the car is still in motion.Read More
Alexandra Foley June 10, 2014
Modernizing the power grid is a huge undertaking. The power industry is mustering up its vast engineering knowledge base to develop the components and systems that will bring us efficient and reliable electrical power for decades to come. By leveraging highly accurate simulation technology with knowledge gained over decades of analyzing in-service equipment, engineers are creating new designs as well as retrofitting proven technology.Read More
Supratik Datta June 9, 2014
Today, we will find out how to compute the total normal flux through a cross-section plane, passing through your simulation geometry. This can help us bridge the gap between simulations and experiments where, in the latter, it is often easier to physically measure the total flux. The approach discussed here works for any type of physics problem as long as we can identify the appropriate flux term corresponding to that physics.Read More
Pawan Soami June 6, 2014
How well you can strike a golf ball is not only determined by your muscle strength, but more importantly — it is influenced by several other factors involved in the mechanics of your golf swing. Let's see how a multibody analysis of a golf swing can be used to improve the outcome of your stroke.Read More


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...In my opinion, religion and spirituality is such a personal thing. Whether any god exists or not, it doesn't really matter. What does matter is how it changes one's life (hopefully for the better). Yes, you could assume that these people are deluding themselves, but who cares? If believing in something makes their life better, that's all that really matters.I think the big problem with religion are churches, which act as an authority. These "leaders" put their own personal spin on things, and speak it as gospel. It's done with good intentions, but it usually will to lead to conflicts in each listeners personal belief. Even worse, they'll change their own personal view to match what's being said. Ugh!....
Oh, and... as for myself, I saw this story a few days ago and had only one thought, which was: Seriously, people are surprised when a scientist expresses religious skepticism?
Mike - I know you were just being cautious but I would like to just say that, and if I recall correctly, the debate that I had with one or two people that went south included some nasty condescension and racial and homophobic tones on their behalf and I don't do well with that sort of rhetoric when I'm directly involved in the discussion. That said, I am not interested in an argument here, besides, I am able to fulfill my communist-socialist left-wing liberal agenda on my personal facebook page.
Actually, E.O WIlson comes to mind as someone who believes in both science and religion. Maybe the divide is less clear if we talking about science and spirituality. In one sense, the two areas are not necessarily mutually exclusive; belief in a unified theory arguably reuqires a certain leap of faith, whether it be in superstrings or in other unknown things that cannot be rationally explained.Forrest


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Lincecum happy '07 trade with Jays didn't occur
ST. LOUIS -- It never occurred. Moreover, it never truly came close to occurring, though media reports claimed that the potential for this event was real.
"It" was the unthinkable: The Giants trading Tim Lincecum to the Toronto Blue Jays for outfielder Alex Rios during the 2007 Winter Meetings. On Tuesday, Lincecum will face Toronto for the first time since an Interleague game during his '07 rookie season. What if he were appearing at AT&T Park as a visiting player?
"I'm happy it didn't happen," said Lincecum, who has made the most of his Giants tenure by compiling an 82-61 record, winning two National League Cy Young Awards and helping San Francisco capture two World Series.
Though some inexperienced players tend to become upset when they hear their name in trade discussions, Lincecum insisted the rumors didn't bother him.
"It didn't have any great significance," Lincecum said. "I didn't read into it too much or think too much about it. For me, it might have been one of those things where it was at the start of my career. ... It was possible that a team could have me so they could trade me."
The rationale behind the proposal was simple. The Giants desperately needed offense, and many industry experts viewed Rios as a budding Albert Pujols after he hit .297 with 24 homers and 85 RBIs in '07 at age 26. But to get a hitter of Rios' apparent skill, any team would have had to part with a prospect like Lincecum, who thrilled Giants fans as a rookie by finishing 7-5 with 150 strikeouts in 146 1/3 innings spanning 24 starts.
The Giants soon declared that Lincecum and Matt Cain would not be traded under any circumstances. But until then, Lincecum said, "Being young, I hadn't established anything."
Chris Haft is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.


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Poly-braid with contrasting polyester ribbon lining. Imported by Wallaroo Hat Company. Size: adjustable interior drawstring. Colors: ivory/ivory, seafoam/tan, light brown/brown, brown/dark brown, sienna/dark brown,black and white/black. UPF 50+, packable. 3 3/4" brim. Handwash cold.
This gorgeous wide-brimmed hat is a perfect accessory for a garden party or an outdoor wedding.


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Mt. Spokane Terrain Expansion APPROVEDPosted On: November 20, 2014 in: Terrain Expansion Project
FOR IMMEDIATE RELEASE:Mt. Spokane Terrain Expansion Approved!Washington State Parks & Recreation Commission has delivered a historic decision on Mt. Spokane's terrain expansion project. This morning at Spokane's City Hall, the Commission voted to classify …+ Read The Story


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Show Highlight
Thursday September 29, 2016
Austin Clarke's "Death of a Panther"
2016 is the 50th anniversary of the birth of the Black Panther Party. The documentary "Death of a Panther" was produced by Canadian writer Austin Clarke in 1969. It tells the tumultuous and violent story of the Black Panthers two months after the death of one of its members, John Huggins.
Rewind Schedule on SiriusXM 169
Should High School Exams be Abolished? The Story of Sue Rodriguez, The Beat Generation, the Battle of the Somme, Max Ferguson, Allan McFee, Frank Zappa, Kierans, Camp and Lewis, Canada Calling, The Food Show and many more treasures from the archives!
Thursday October 20, 2016
The Sixties Scoop
Imagine a child taken away not just from her family but from her culture and raised in a family that may or may not treat her well. She might feel a little lost. That was the experience of up to 20,000 indigenous children between the mid 1960s and 80s. It was called the Sixties Scoop and it's the story on this edition of Rewind.
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