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Talk:Knight:Beta-galactosidase assay/96 well format
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< Talk:Knight:Beta-galactosidase assay
Revision as of 18:59, 30 October 2007 by Reshma P. Shetty (Talk | contribs)
This is an outline of various control experiments that I need to do. It is a work in progress and has not been done.
A600 versus cell density
- Grow an overnight culture to saturation in EZ Rich Media
- Pellet the cells
- Resuspend in 1/4 of the original volume with EZ Rich Media
- Add 350 μL of cell suspension to the first well
- Add 175 μL of previous well to next well.
- Add 175 μL of EZ Rich Media to that well.
- Repeat dilution series until the well's solution looks totally clear.
- Add an additional well of 175 μL EZ Rich Media.
- Measure A600 of each well in the plate reader.
- Plot A420 versus dilution factor. This relationship should be linear.
Effect of evaporation on absorbance readings
Effect of evaporation on A600 readings
- Aliquot 50 μL of culture into an entire row of wells.
- Do duplicates rows to assess measurement variability in duplicate samples.
- Aliquot 100 μL of a of culture into a second row of wells.
- Add increasing volumes of water to each well (by 10 μL increments).
- Measure the A600 of the plate.
Effect of evaporation on A420 readings
- Aliquot 50 μL of a set concentration of ONP into an entire row of wells.
- Could add different concentrations of ONP to different rows.
- Add increasing volumes of water to each well (by 10 μL increments).
- Measure the A420 of the plate.
β-galactosidase activity versus number of cells
- Do parallel β-galactosidase assays with a variable volumes of cells from the same grown culture.
- 1 = 1 μL of cells
- 2 = 2 μL of cells
- 3 = 3 μL of cells
- 4 = 4 μL of cells
- 5 = 5 μL of cells
- 6 = 5 μL of media
β-galactosidase activity versus growth phase of culture
- Grow an overnight culture of several constructs
- H = Media+IPTG+AHL
- In the morning, dilute back samples into EZ Rich Media to an A600 of 0.001 (via a Nanodrop reading) in a 96 well plate. (Each well in a row should be identical with 175 μL of culture according to the list above.)
- Let grow 1 hour.
- Add IPTG and/or AHL to rows as appropriate.
- Prepare the permeabilization buffer and aliquot 80 &muL into each well of a 96 well plate.
- Using a larger volume for permeabilization step because the samples are sitting for a while.
- Measure the A600 of the plate every 30-60 minutes or grow the plate in the plate reader and pause it every 30-60 mins.
- Use a gas permeable plate cover. Leave this on for absorbance measurements?
- At each hour point, take 20 μL of the next column of culture and add it to the corresponding columns of the permeabilization plate.
- Once each time point has been taken, move 25 μL of each well from the permeabilized culture to a new plate.
- Add 150 μL of substrate solution to each well.
- Place plate in the plate reader to measure change in A420 as a function of time.
- Plot the β-galactosidase activity in Miller Units as a function of the A600 of the culture.
A420 versus o-nitrophenol concentration
- Make up a solution of 14 mL to control for background absorbance in β-galactosidase assays
- 1600 μL permeabilization solution
- 12 mL substrate solution without ONPG
- 400 μL EZ rich media supplemented with kanamycin and AHL
- Make up 1mL of 1 mg/mL ONP.
- Add 350 μL of 1mg/mL solution to the first well.
- Move 175 μL of previous well to next well.
- Add 175 μL of background solution to that well.
- Repeat dilution series until the well's solution looks totally clear.
- Add an additional well of 175 μL background solution.
- Measure A420 of each well in the plate reader.
- Plot A420 versus ONP concentration. This relationship should be linear.
Concentration series:
1mg/mL -> 0.5 mg/mL -> 0.25 mg/mL -> 0.125 mg/mL -> 62.5 μg/mL -> 31.25 μg/mL -> 15.625 μg/mL
-> 7.8125 μg/mL -> 3.90625 μg/mL -> 1.953125 μg/mL -> 0.9765625 μg/mL


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NATO Senior Civilian Representative provides script for transition in Afghanistan
On 13 October, the NATO Senior Civilian Representative (SCR) provided the media with a script of the transition process to Afghan lead.
At a roundtable, which followed a briefing to the International Security Assistance Force (ISAF) troop contributing nations, Ambassador Sedwill insisted that the transition process will be conditions-based, not calendar-driven, and follow a comprehensive approach, where all aspects of security, governance and development will be taken into account.
The transition process is expected to start in the spring of 2011 and be completed by the end of 2014, by which time the Afghan authorities will have taken the lead throughout the country. However, the first tranche of areas candidates for transition will not be announced at the Lisbon Summit, Ambassador Sedwill stressed.
"Doing this months ahead of the starting date could pose sound security risks," he explained, "and it is for Afghanistan, as a sovereign nation, to make such an announcement."
While it is estimated that it might take up to 24 months to fully transition one given area to Afghan lead, conditions on the ground will dictate the pace at which transition will be carried out. "All these timelines should not be seen as a prediction but as a commitment," he underlined.
Four criteria are to drive the transition assessment: the overall security situation; the capacity of the Afghan national security forces; ISAF and Provincial Reconstruction Teams' (PRT) readiness to shift to a supporting role; and progress with governance and development. It will follow a two-pronged approach, involving key Afghan institutions and functions as well as geographic areas.
"This could lead to a situation whereby transition starts at the sub-district level with the transfer of lead security responsibility to the Afghan National Police" along the lines of the successful transfer of lead security responsibility that occurred in Kabul mid-2008, Ambassador Sedwill explained.
On the 2014 horizon, "we will see changes on the ground as a result of transition," he indicated. From a security point of view, ISAF troops will become gradually less visible, thinned out and may be redeployed within the country or reinvested as part of training efforts. Provincial Reconstruction Teams will also evolve towards a greater civilian role, focusing on a more traditional model of development delivery.
"In sum, transition is all about Afghan leadership and international partnership," Mark Sedwill concluded.

subset: commoncrawl


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Third post in a series. In case you missed it, here are the first and second.
Technological infrastructure for social learning
Work and learning are converging, and as this change happens, the infrastructure of the old corporate learning must go – things like traditional one-size-fit-all in-person training seminars. In its place enters social and informal learning hubs like on-demand content, live online discussions, wikis and forums, and searchable content archives. The great news is that social and informal learning don't require new systems because learning can take place on the same "platform" as the existing social network, if a company already has one.
The primary thing to bear in mind, says MIT's Andy McAfee (McAfee), is INATT. That's short for a phrase that kept coming up in conversation when he was writing Enterprise 2.0. It's short for "It's Not About The Technology." People come first.
But you can't do without the technology either. Social networks are the ideal platform for the new corporate learning, so let's briefly examine how they support corporate learning.
Social computing
Early personal computing was based on corporate computing. Conventions like ASCII, programming languages, Internet protocol, and encryption were developed for corporate mainframe computers and only later adopted for personal computers. That situation has flip-flopped. Innovations in applications and user-interface design are born on the consumer side and migrate to the enterprise.
Forbes named Salesforce.com the world's most innovative company. Where did that innovation come from? Salesforce.com says cloud-based Customer Relationship Management application borrowed heavily from Amazon. Salesforce.com's social network application was inspired by Facebook. Salesforce.com's Chatter began its life as in-house Twitter. As the web turns social, Salesforce.com has changed its mission to "leading the shift to the Social Enterprise," and that's where it's proving its forward-thinking nature.
So how do you find the right social platform to enhance your corporate training program? When an organization is improving its workscape, looking at consumer applications is a good way to think about what's required in the corporate space. Ask net-savvy younger workers how they would like to learn new skills, and they bring up the features they enjoy outside of work:
- A personal profile so I can share information with my connections
- A personalized experience and recommendations, like Amazon
- Connections to friends and colleagues, like Facebook and LinkedIn
- Activity streams, like Twitter, so I know what's going on and what people are talking about
- Face-to-face interaction and desktop sharing through video conferencing
- Multiple access options, like a bank that offers access by ATM, the Web, phone, or human tellers
- A diverse learning library, made up of videos, FAQs and links to relevant information
- Single sign-on access, like using my LinkedIn profile to access other programs
- Choosing and subscribing to streams of information I'm interested in
- Provide a single, simple, all-in-one interface, like that provided by Google
- Make it easy to share photos and video, as on Flickr and YouTube
- Leverage "the wisdom of crowds," by allowing me to pose a question to my connections
- Enable users to rate content that they liked the most or found the most helpful
Minimum viable workscape
What we're talking about is a social work hub where every employee and external partner can come to collaborate, share information, get information and provide updates and ask questions. When it comes time to build your new collaborative and social learning center, some of those consumer applications are simple to replicate in-house. Others are not. You probably can't afford, and definitely don't need, to create your own Facebook or Google behind your firewall. There are lots of applications you can implement at reasonable cost. Be skeptical if your collaborative infrastructure doesn't include these minimal functions:
Profiles – so each employee can personally connect to the network. Profile should contain photo, position, location, email address, expertise (tagged so it's searchable). Nice-to-haves include how to reach you (noting whether you're online now), reporting chain (boss, boss's boss, etc.), link to your blog and bookmarks, people in your network, links to documents you frequently share, members of your network.
Workspaces – to break up the organization's activity into relevant, digestible feeds for each individual and feeds. Workspaces are networks within the organization that are created by employees to gather a team or group in a specific area. For example, new hires that are brought on at the same time, may create a workspace where they can ask each other questions and share information that they find out.
Activity stream – for monitoring the organization pulse in real time, sharing what you're doing, being referred to useful information, asking for help, accelerating the flow of news and information, and keeping up with change. Activity streams should be available for the company at large and for workspaces.
Wikis or notes – for writing collaboratively, eliminating multiple versions of documents, sharing information with a relevant group, eliminating unnecessary email, and sharing responsibility for updates and error correction.
Integrated virtual meetings – to make it easy to meet online, because there needs to be room in your learning program for group discussion and application. Minimum feature set: shared screen, text chat, video conferencing streams.
Mobile access – Half of America's workforce works away from the office at least sometimes. Smart phones are surpassing PCs for connecting to networks for access and participation. People post more Tweets via phone than via computers. Google designs its apps for mobile before porting them to PCs. What does all of this mean? Your new social workscape needs to be mobile so people can collaborate from anywhere.
Putting a learning platform in place
When it's time to put a learning platform in place, it's a good idea to make a company wide commitment to your new philosophy on learning. Here's an example from a company I recently worked with:
- We are open and transparent
- We narrate our work. Need to share.
- We offer live and on-demand training content as a part of continuous learning
- We value conversation as a learning vehicle
- We make our work accessible to others
- We are a vanguard of change within the company
- Our bottom line is business success
- We know learning is work; work is learning
- We are a learning organization
- We value time for self-development and reflection
- We recognize that reflection is a key to learning
Changing behavior requires continual reinforcement, so be ready to tackle the concern and resistance that some people may have toward becoming a more collaborative organization.
A great way to embrace your new collaborative nature while helping people adapt to it, is to host all-hands virtual meetings to share your process toward becoming a collaborative organization. Make your employees a part of the evolution; keep them in the loop.
Learning Networks
Networks are not only the environment of learning; they're also the place where problems are solved, discoveries are made, and new knowledge is created.
This way of looking at learning platforms builds on
the work of Harold Jarche and the Internet Time Alliance.
Workers are members of multiple, interconnected networks.
Everyone has personal face-to-face networks: the friends, neighbors, colleagues, and acquaintances we talk with. Most people have electronic personal networks, too: Facebook, discussions groups, and a variety of followers and followed comrades. We rely on our networks to help us learn what's going on in our worlds. The collaborative organization may replicate those personal connections through social work platforms with customizable workspaces. Each workspace is for a group of connected people – teams, departments, project contributors, and so on.
Communities are networks of people who share common interests and identify themselves as cohorts. A community may be a group of professionals (e.g. chefs or chip designers) or people with shared passions (e.g. model railroaders and cyclists) or co-workers from different work teams (e.g. the United Way Committee or neighborhood watch). Communities share knowledge ("Here's a great recipe for crayfish with foie gras"), help one another ("There's an opening for a sous-chef at the Fish Trap in Key West"), validate best practices ("Use coddled eggs in Caesar salad to avoid salmonella"), and develop apprentices into professionals ("My salad chef is ready to become a pastry chef"). Communities can exist internally (the United Way Committee) or externally (the chefs). Innovation in Silicon Valley is enhanced when competitors share trade secrets because allegiance to their professional community ("We're chip designers") is strong than to their employer ("I work for AMD.")
Many companies enable workers to establish a personal node in the company's social platform. This is where your individual profile enables people to find you, know what your good at, and share things you may be interested in. Many workers narrate their work on individual blogs. Transparency builds trust.
Most information work is carried out by project teams. When team members are unable to meet in the same physical space, they rely on networks to collaborate on getting projects done. Team members who work together, learn together. In time, team members develop strong social ties, trust emerges, and they co-create new knowledge and innovation. Experience is the best teacher and work teams are where it happens.
Project Teams have a job to do; communities come together to cooperate and share for the good of the group. Project teams inevitably need to acquire knowledge from outside their small circle. Their individual members are often members of several communities, which they tap for knowledge and guidance. A smart organization supports its internal teams and encourages its people to take part in external teams.
Many progressive companies have set up social work platforms that connect all employees to an activity feed that lists activities and pointers from all over the company. Social workspaces are the ultimate silo busters, enabling everyone to be on the same page, accelerating the organization's cycle time, and letting "the company know what the company knows."
A Note About Internet Access
Many companies signal their lack of trust in their employees by denying them access to the greatest assembly of knowledge in the history of humanity, the Internet. To be consistent, they should probably take away their telephones (They might make long distance calls to China!) and pencils (They might waste time playing tic-tac-toe). Bad apples are going to do bad things with or without the Internet, but by hoarding access to the web, you're not only punishing your good apples, but also hindering their ability to learn.
For many people today, working without the net is equivalent to working blindfolded. When companies deny access to the net, employees route around them with smartphones and tablets that bypass corporate IT. The price of criminalizing access to the net is lower morale, the message that it's okay to break rules (wink, wink), and to give up on hiring the best and the brightest (who will work somewhere they are trusted to act like responsible citizens). Companies should encourage workers to connect to the outside world, for that's where the customers are.
The Internet is an essential library of information for today's workforce. David Weinberger points out that the web has changed the nature of knowledge itself. Knowledge that was once limited to what you could print on a page is now connected to all manner of evidence, counter-claims, elaboration, and interpretations.
The basic idea is that the properties of knowledge that we've taken for granted at least in the West for, oh, 2,500 years are not actually properties of knowledge. They're properties of knowledge when its medium is paper. And when you remove the paper and put things online, it takes on the properties of its new medium—of the Internet. Importantly, knowledge in a network includes differences and disagreements in a way that traditional knowledge is uncomfortable with. Everything is unsettled, everything is argued about, and very few things are ever totally resolved on the Net.
There's a word for companies that deny workers access to the riches of the Internet. That word is stupid.
The next post in this series will address readiness for and benefits of collaborative learning.


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Please bare with me as the spacing on this article is horrible and i have trouble with it. Anyone can help me with that? Daniel Bryan vs Dolph Ziggler - WWE Championship (Iron Man Match) Winner: Dolph Ziggler John Cena vs The Undertaker: The Face vs The Streak Winner: The Undertaker The Shield vs The Wyatt Family: Losing Team Disbands Winners: The Wyatts The Rock vs Brock Lesnar:
Updated 09-30-2013 at 08:19 PM by RatedRKO
Darren Young is one half of the Prime Time Players, a heal tag team that would do the job every week. Then one day from the airport from TMZ, Darren Young announced that he was a homosexual. Then everything changed. The entire tag team turned face. Fine. Darren Young's fist singles victory was against Antonio Cesaro. That is where I cross the line. I felt like WWE has really treated this the wrong
The Shield and The Wyatt Family are the future of WWE. These two trio factions have made their present felt in distinct ways. But now that they have each gotten at least one PPV appearance I think a comparison is in order. And I am here to do just that. The other day I called The Wyatt Family Bray Wyatt and The Hillbillies but they will now be compared as a unit. I will be comparing everything. And
This could have been a special party of the summer. This could have been the PPV that we have all been waiting for. But because of WWE's lackluster creative team, we will succumb to another PPV that makes me resort to watching pay-per-views via online streams. When I look at the card, there are only three matches I'll be tuning into:
Kane vs Bray Wyatt
John Cena vs Daniel Bryan
Updated 08-15-2013 at 04:14 PM by RatedRKO
WWE has actually decided to add a curveball to this year's Money In The Bank Pay-per-view: unpredictability. Last year's winners were John Cena and Dolph Ziggler, two obvious choices. But this time, they are making us guess at who could win, why they should win, and whether or not curveballs will be thrown. Also, we have, for the first time, a Money In The Bank ladder match with all heels. I don't
Updated 06-26-2013 at 08:10 PM by RatedRKO
Yes, you read that correctly. Adam Copeland, A.K.A Edge was the greatest superstar of the past decade. That's right, no Triple H, no Shawn Michaels, no Undertaker and certainly no John Cena. I write this article with a burning certainty as well as an unbiased mind. Now if you have read the article up to this point i beseech you to continue reading this article. Hear me out. I am going to back up my
So Payback has finally dawned upon us and it seems that there is much to talk about. CM Punk will make his return in front of his hometown crowd, Randy Orton and Daniel Bryan will be teaming up, John Cena and Ryback will compete in 3 STAGES OF HELL, Wade Barrett actually gets to be on the show this time, there's a Divas MATCH and, ah...who am I kidding? Let's just get down to the predictions.
WWE presented us with its fourth PPV of the year, Extreme Rules. The card looked impressive, but did it deliver? Well, last night I ordered a pizza and watched the whole thing and boy do I have much to say. I may be a critical person but if i didn't see anything wrong with it I wouldn't be making my complaints. So here we go.
Pre Show: The Miz vs Cody Rhodes
This match
It's that time of the year. The only time of the year where every WWE superstar gets extreme. To be honest, this PPV is looking a lot better than WrestleMania and that is no exaggeration. While this past week's Monday Night RAW, with the exception of The Shield match and the Triple H/Lesnar segment, was uttlerly atrocious, this PPV has a lot of potential. We have a Steel Cage match, an I Quit Match,
It looks like someone has an affiliation for current WWE Divas Champion Kaitlyn. To me, Kaitlyn is a fine-looking woman, so this mystery is sure to be great. We even got The Great Khali (A.K.A - The Indian Sherlock Holmes) on the case. Who could it possibly be? This has the makings of becoming the greatest mystery in WWE HISTORY! Is it Cody Rhodes? We have seen him flirt with Kaitlyn before. How about
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Adapt or Be Crushed
Since the market low in March 2009, one of most consistent tendencies of the market has been to bounce and keep going. In the old days you could actually fade an oversold bounce aggressively, but now you are likely to be crushed if you have any doubts about the market's ability to turn back up and keep on running.
I suspect that this change in behavior has something to do with high levels of liquidity and more aggressive computerized trading. It is obvious that normal human emotions don't tend to swing to the extent we see these days. We often hear that the market has no memory from day to day because the computers are programed that way....167 more words left in this article. To read them, just click below and try Real Money FREE for 14 days.
There's no substitute for a trading floor to get great ideas, so Jim Cramer created a better one at Real Money and blogs there exclusively. We then added legendary hedge fund manager, Doug Kass, with his exclusive Daily Diary and best investing ideas. Staffed with more than 4 dozen investing pros, money managers, journalists and analysts, Real Money Pro gives you a flood of opinions, analysis and actionable trading advice found nowhere else, and allows you to interact directly with each expert.
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March 12 - Before 4 p.m. EDT, clubs must exercise options for 2013 on all players who have option clauses in their 2012 contracts; clubs must submit qualifying offers to their restricted free agents with expiring contracts and to whom they desire to retain a right of first refusal/compensation; clubs must submit a minimum salary offer to retain exclusive negotiating rights to players with expiring 2012 contracts and who have fewer than three seasons of free agency credit; all 2012 player contracts expire. All clubs must be under the salary cap. The 2013 league year, free agency and trading period begins at 4 p.m. EDT.
March 17-20 - Annual league meeting, Phoenix
April 25-27 - NFL draft, New York
May 20-22 - NFL spring league meeting, Boston
Sept. 5, 8-9 - 2013 NFL season begins.


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HQ Inside Sales Advisor â€" European Headquarters, Amsterdam
About Tesla
Palo Alto, California-based Tesla Motors designs and manufactures Electrical Vehicles and EV powertrain components. Tesla's goal is to produce a full range of electric cars from sports cars to mass-market vehicles and we are determined to decrease the cost of electric vehicles. We have designed, produced and delivered Tesla Roadsters to customers in North America, Europe and Asia Pacific. Model S, the world's first premium electric sedan is now being rolled out world-wide.
The Role
The primary focus of the Product Specialist team is responding to customer inquiries about the purchase of new vehicles in a timely and accurate manner. The HQ Product Specialist team will focus on Vehicle Configurations and will be working with existing Reservation holders to convert their reservation in to a production order. The HQ Product Specialist is responsible for these customer service activities in order to deliver top quality customer service and customer satisfaction.
Tesla Motors is committed to a policy of equal employment opportunity. We recruit, employ, train, compensate, and promote without regard to race, color, age, sex, ancestry, marital status, religion, national origin, physical or mental disability, sexual orientation, gender identity, medical condition, pregnancy, veteran status or genetic information
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The SaberCats announced their AFL game against the Tampa Bay Storm has been moved from May 11 to May 10 at 7:30 p.m. in HP Pavilion.
The change is due to a potential scheduling conflict involving Game 6 of the first-round NHL playoff series between the Sharks and Vancouver Canucks.
"We are glad to accommodate this change," SaberCats managing partner Dave Fry said Thursday.
The SaberCats (3-2) play host to Cleveland (1-4) on Saturday night.
The Earthquakes waived forward Alexandre Gonzalez Emerson and midfielder Jacob Hustedt and activated midfielder Mehdi Ballouchy from the disabled list. Ballouchy, a former Santa Clara All-American, has been out since tearing the anterior cruciate ligament in his left knee against FC Dallas on Sept. 29, 2012. Gonzalez, 20, trained with the Quakes during the 2013 preseason, and the San Jose native signed with the club April 4. He made one appearance in Reserve League play.
The match marks the fourth straight year the Quakes have hosted an English Premier League team for a summer friendly. "This match is another great opportunity for Bay Area soccer fans to watch a top-notch opponent play against their local club," Quakes president Dave Kaval said. Ticket information is at www.sjearthquakes.com or 800-225-2277.
Horse racing
Moonshine Bay recorded another win under trainer Jerry Hollendorfer at Golden Gate Fields, taking a $34,816 sprint feature by 21/4 lengths. Moonshine Bay has made four starts at Golden Gate Fields since being bought by Hollendorfer, Marvin Kirsner, Al Morrison and George Todaro in January. Moonshine Bay has three wins and a second-place finish in the Albany Stakes. Dennis Carr rode Moonshine Bay in six-furlong allowance race, finishing in 1:10.12.
Santa Clara, UNLV and Florida Gulf Coast headline the field for the 13th Las Vegas Classic. Each basketball team will play four games — the first two at on-campus sites and the final two rounds at Orleans Arena on Dec. 22-23. Also in the field are Mississippi State, South Florida, Florida A&M, Radford and Sacred Heart.
Kennedy was an assistant at USF the past five seasons, while Lowery spent the past four seasons as an assistant at Cal Poly.


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It was a bad night in the Ryman League for Heybridge Swifts, Maldon and Tiptree and Burnham Ramblers.
All three sides fell to defeat in their Ryman League division one north fixtures.
It was the battle of the brothers as Needham Market's Sam Newson took on Swifts Ben Newson Ben Newson went close for Swifts on 20 minutes when he was denied by a good block.
But it was brother Sam that broke the deadlock four minutes later with a volley over Ashley Harrison in the Swifts goal.
Sam Newson then went close to doubling Needham's lead a few minutes later.
But Heybridge equalised on the stroke of half-time when Jamie Guy, on his return to Needham, glanced home a Danny Barber free-kick.
Needham had two penalty appeals waved away in the second half much to the anger of the home team.
But on 81 minutes they were awarded a spot kick after a foul on Ollie Fenn and former Colchester United player Kem Izzet sent Harrison the wrong way for a 2-1 win.
Maldon and Tiptree lost out 2-1 at Ware.
The visitors fell behind to an early goal when Kieran Bishop struck in the ninth minute.
Maldon found themselves 2-0 down when Bishop added another five minutes later.
Bradley Hunter pulled a goal back for Maldon on 75 minutes.
Ware went down to ten men on 90 minutes when Murat Karagul was sent off but the visitors couldn't take advantage to find an equaliser.
Burnham Ramblers fell to a 5-2 defeat against visiting Harlow Town.
Ramblers fell behind in the second minute of the game when James Smith fired home.
But the home side didn't leave it long to draw level when Dan Williams equalised two minutes later.
However, the scoreboard was troubled again on ten Minutes when Alex Read regained the lead for Harlow.
Junior Dadson then made it 3-1 as the visitors stretched their lead on 14 minutes.
After the early flurry of goals it wasn't until the second half that Burnham reduced the deficit when Billy Hunt pulled one back.
However, Read restored the visitors two-goal cushion on 72 minutes.
And Dadson made it worse for Ramblers on 90 minutes when he made it 5-2.


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Risk Management
Risk Management for Student Organizations and Advisors
In response to House Bill 2639, a Risk Management training program has been developed.
All student organizations should be concerned with risk management. No matter how big an event or how small an activity; risk will be present at some level. Learning to manage risk will reduce individual liability and ensure a fun, safe environment for participants.
Risk Management is the process of considering the potential and perceived risk involved in activities. It includes monitoring organization activities and taking both corrective action and proactive steps to minimize accidental injury and/or loss.
This website will provide you with information to help identify risk and take proactive steps before difficult situations arise.
Training Dates
Each recognized student organization is required to designate a risk management officer. It can be a stand alone position or duties encumbered into another office. The risk management officer is required to complete the risk management training annually. If the risk management officer has been trained previously, he/she has the option of re-certifying by reviewing the risk management information on the Student Activities website and submitting a Statement of Completion to our office.
If the risk management officer fails to attend training, activities deemed risky will not be approved. If the risk management officer resigns or is removed from his/her office, Student Activities will work with the organization to become compliant.
All organization advisors must attend a one-time training session. Training will carry over from year to year.
Fall 2017 dates will be announced soon
Once an executive member attends a risk management session, he/she must relay all information to their organization and submit a Statement of Completion to Student Activities. Information to properly train your organization is found under Training Your Organization. Organizations are required to update their risk management policy every 5 years. If you are unsure how to do that, you may refer to Adopting a Risk Management Policy. For verification of your organization's risk management policy, contact Student Activities.
The following links will provide you with additional information, PowerPoints, and websites regarding risk management issues.


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Maternal Lifestyle and Neonatal Hypoglycemia
- Full Text View
- Tabular View
- Study Results
- Disclaimer
- How to Read a Study Record
tPA has a pivotal role in placentation, mediationg activation of growth factors, such as vascular endothelial growth factor and brain-derived neurotrophic factor, degradation of extracellular matrix and basement membrane (directly or through activation of matrix metalloproteinases) and formation of hemidesmosomes.
A high-carbohydrate intake combined with lack of physical activity provides a strong stimulus for maternal insulin production. In this scenario, either β-cells are dysfunctional and diabetes supervenes, or excessive amounts of insulin are produced, providing pathological stimulation of PAI-1 synthesis. Given that PAI-1 is a major tPA inhibitor, PAI-1 excess may affect placentation, increasing the risk of first trimester losses, preterm deliveries and intrauterine growth restriction.
Our hypothesis was that prematurity was not the cause of neonatal hypoglycemia, but a parallel occurrence of a strong stimulus for maternal, fetal and neonatal production of insulin.
|Hyperinsulinemia Miscarriages Pregnancy Sedentary Lifestyle Hypoglycemia
|Behavioral: Daily brisk walking plus a carbohydrate-restricted diet
|Study Type:
|Study Design:
|Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single (Participant)
Primary Purpose: Prevention
|Official Title:
|Repercussion of Maternal Lifestyle on Obstetric and Neonatal Outcomes
- Neonatal Hypoglycemia [ Time Frame: 1, 2 and 4 h after birth. ]Any glucose level equal or below 40mg/dL at 1, 2 or 4 h after birth, obtained by heelstick.
- Refractory Hypoglycemia [ Time Frame: One hour after feeding or after intravenous dextrose ]
Any glucose level ≤ 40/dL at 1, 2 or 4 h:
- Neonates with hypoglycemia (glucose level equal or below 40 mg/dL at 1, 2 or 4 h) will be offered milk. Neonates unable to suckle, will be treated with intravenous dextrose for one hour.
- A new heel stick blood sample will be drawn to assess glucose levels.
- Neonates with persistent hypoglycemia will be considered as refractory hypoglycemia.
- Pregnancy and Neonatal Outcomes [ Time Frame: Three years ]Early miscarriages, 2nd and 3rd trimester losses, preterm deliveries, take-home babies, neonatal hypoglycemia: number of babies
|Study Start Date:
|March 2011
|Study Completion Date:
|October 2015
|Primary Completion Date:
|April 2013 (Final data collection date for primary outcome measure)
|Assigned Interventions
Active Comparator: Lifestyle counseling
Daily brisk walking plus a carbohydrate-restricted diet
Behavioral: Daily brisk walking plus a carbohydrate-restricted diet
Daily brisk walking at moderate speed (4 km/h) for at least 40 minutes per day, 7 days a week. Patients will be recommended to avoid high-glycemic index meals (such as snacks, candies, fiber-free juices and sugar-sweetened beverages), and to eat at least two daily servings of meat, poultry, fish (e.g. 2 g/kg) or other protein-rich food, starting when they decided to get pregnant and continuing until delivery. Recommendations will be emphasised at every appointment. Antidepressants will not be discontinued in both groups, but patients on paroxetine and sertraline, will be switched to fluoxetine.
No Intervention: Standard follow-up
Prenatal care will proceed according to the routine.
Detailed Description:
In an observational study, we sought to determine whether markers of hyperinsulinemia or situations that increase maternal insulin requirements would increase the risk of neonatal hypoglycemia. Mothers were selected if they had grade III obesity, acanthosis nigricans (surrogates of chronic maternal hyperinsulinemia), any invasive bacterial infection or if they had used corticosteroid within seven days before delivery (surrogates of subacute insulin resistance), if they reported to have consumed a high-glycemic index diet within 24 hours before delivery or if they were physically inactive within 24 hours before delivery (conditions that could increase maternal insulin requirements close to delivery).
Based on the finding that that the risk of neonatal hypoglycemia increased fivefold with inactivity (95% CI: 2-11, P <0.001), 11-fold with high-carbohydrate intake (95% CI: 4-24, P <0.001) and 329-fold with both risk factors (95% CI: 32-3362, P <0.001), next we have evaluated how a protocol combining exercises and a balanced diet throughout pregnancy influences maternal and neonatal outcomes. One of the outcomes analyzed was neonatal hypoglycemia.
|Ages Eligible for Study:
|up to 40 Years (Child, Adult)
|Sexes Eligible for Study:
|Accepts Healthy Volunteers:
Please refer to this study by its ClinicalTrials.gov identifier: NCT01409382
|Hospital Federal dos Servidores do Estado
|Rio de Janeiro, RJ, Brazil, 20221-903
|Principal Investigator:
|Maria A Sayeg-Porto, MD, PhD
|Hospital dos Servidores do Estado, RJ; Universidade Federal do Rio de Janeiro
|Principal Investigator:
|Paulo R Benchimol-Barbosa, MD, DSc
|Universidade Gama Filho; COPPE/UFRJ
|Principal Investigator:
|Silvia Hoirisch-Clapauch, MD
|Hospital dos Servidores do Estado, RJ
Additional Information:
|Responsible Party:
|Silvia Hoirisch Clapauch, Principal investigator, Hospital dos Servidores do Estado do Rio de Janeiro
|ClinicalTrials.gov Identifier:
|NCT01409382 History of Changes
|Other Study ID Numbers:
|Study First Received:
|May 27, 2011
|Results First Received:
|April 26, 2013
|Last Updated:
|August 19, 2016
|Individual Participant Data (IPD) Sharing Statement:
|Plan to Share IPD:
Keywords provided by Silvia Hoirisch Clapauch, Hospital dos Servidores do Estado do Rio de Janeiro:
neonatal hypoglycemia
physical activity
sedentary lifestyle
high-glycemic index diet
take-home baby
recurrent miscarriages
preterm delivery
Additional relevant MeSH terms:
Abortion, Spontaneous
Glucose Metabolism Disorders
Metabolic Diseases
Pregnancy Complications
ClinicalTrials.gov processed this record on September 21, 2017


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The best thing about an old car is that it's paid for. To be frank, that's the only good thing about an old car, if it has an eternally glowing check-engine light and the window crank keeps falling off, like a certain 2000 Jetta TDI that I would be ashamed to show you.
Eleven years. That's the average age of an American car, and drivers are doing something about it: They're buying. Auto sales in August were their strongest since May 2007.
In this week's podcast:
- Jeremy Acevedo, analyst for Edmunds.com, describes the state of the auto market and explains why leases have been good deals lately.
- Bankrate editor Doug Whiteman tells about companies that help employees get training that offers college credit.
- David Nice, economist for Mesirow Financial, explains why he believes that interest rates have plateaued for a while.
- And Bankrate reporter Janna Herron gives a first-person account of what happened when she corrected a glitch in her credit report. You wouldn't believe how much the correction raised her score.
Bankrate Audio
What's hot now that car sales are the best in years?
With cars and trucks selling briskly, can you still deal? And, what are the chances that interest rates are going to jump?
Audio Transcript
Mark Hamrick: From Bankrate.com, this is "Your Money This Week." I'm Mark Hamrick reporting from Washington.
August was hot, but not like you might think. We're talking car sales. We'll check in with industry expert Jeremy Acevedo to learn why car buyers are on the move. Economist David Nice with Mesirow Financial gives us a sense about what's next with the U.S. economy. He suggests we could get caught flat-footed when interest rates really do start to move higher. Bankrate's Doug Whiteman tells us how workers are saving on tuition thanks to their employers, and our Janna Herron tells the story how a disputed bill threatened to stand in the way of her own home purchase. We will have all of that, and more for you on "Your Money This Week."
In our first segment, the market for new cars and trucks is revving up to be the best in half a decade. Its consumers, of course, providing the gas power or hybrid, or electric. Jeremy Acevedo is an analyst for Edmunds.com. I asked him to help put newly released August car sales figures in perspective.
Jeremy Acevedo: It was fantastic, in fact, one of the best we have seen in quite a while. Sales topped 1.5 million, which it has not done since prerecession, May 2007 actually.
Mark Hamrick: What is behind this renewed enthusiasm on the part of American car buyers?
Jeremy Acevedo: Well, I think that's part of the story. This is primarily done on the back of retail sales, so it's not really being buoyed by weak sales like we have seen in the past. This is actually consumers moving in to dealerships and actually purchasing cars. And I think there's a confluence of factors, but specifically credit availability, the availability of money, consumer confidence, and just some cars that are really bringing customers into the dealerships. And couple that with the fact that cars right now are some of the oldest on the road that we have ever seen, so as durable goods, they need some replacement and with consumer confidence rising, consumers are getting back into those dealerships and buying cars.
Mark Hamrick: OK, so I guess sedans are doing very well. I guess we are also seeing some renewed interest in SUVs and trucks. So break that down for us about what kind of vehicles people are buying.
Jeremy Acevedo: Absolutely, trucks, I think have been the big story. Through the recession, there was a bit of a lull in large truck sales, but now with the housing recovery, large truck sales have seen a big boom, so they are sitting at 12 percent of the market right now, which is really strong; stronger than we have seen in the past, definitely. Sedans, definitely. In fact, interestingly this month with compacts ... compacts are actually taking the top spot from midsized cars, which is fairly rare, but between compacts and midsized cars, definitely the most popular cars out there.
Mark Hamrick: That's interesting, so on the part of the large trucks, this is essentially construction workers who may be either going back to work after the housing market was flat lining it. I can imagine some people are buying them for every day needs, but it sounds like a lot of this is industry related.
Jeremy Acevedo: Absolutely, with the housing, a lot of those purchases are need-based and the actual construction of homes and housing starts. But additionally, it's just one of those things that goes along with the consumer confidence of owning a home. It's the home purchases and homeownership, also lead to truck sales.
Mark Hamrick: So when it comes to either vehicles or manufacturers, what's doing well out there?
Jeremy Acevedo: Interesting again, this an across-the-board boom for the industry, but specifically, the Japanese automakers made out particularly strong in August. Toyota had their best month since May 2008, so we are stretching back to those prerecession dates. Lexus actually, in a pretty rare twist, is the best-selling luxury make this month, so that's usually a spot occupied by Mercedes or BMW, those two have been kind of running back and forth, but Lexus has actually moved into the top spot. It was a big month for Camry, as far as Toyota goes. Honda and Nissan, they had similar success as far as sales.
Mark Hamrick: OK, and I notice also that you folks are saying there at Edmunds that leases are doing pretty well.
Jeremy Acevedo: Leasing has been strong, certainly driving a lot of sales in the market right now. Right now we are seeing right about a quarter of all sales are leases, and that's the highest we have seen certainly in the last decade.
Mark Hamrick: So, how do the manufacturers figure out the pricing on that? I know just watching commercials, I've seen some lease offers that seem like, "Hey, that's a pretty good deal" for somebody who wants to have a relatively low payment for a pretty nice vehicle in many cases. How do the manufacturers price that relative to where someone wants to buy a vehicle outright?
Jeremy Acevedo: The great news is that those are some astute observations there because there are some great deals out there as far as leasing goes. What's really driving that is the residuals. The residuals of these vehicles ... cars have actually seen a great lift in quality and durability in resale value. So, consumers are actually benefitting from manufacturers being able to rely on the increased resale value in a subsidized lease.
Mark Hamrick: Very often, when sales are strong, it seems like the incentives the manufacturers are offering can be reduced. Now, if cars are the best they've been since the recession, if car sales are the best they've been since the recession, 2007, does that mean that dealers and manufacturers might be less willing to bargain, so to speak?
Jeremy Acevedo: It doesn't seem that way so far. What we are actually seeing is that it's becoming an increasingly competitive landscape, and so we see it looks like the incentives are staying just about the same.
Mark Hamrick: How should a consumer go armed, let's say to a dealer, with information in this, now that we have had the Internet around for a couple of decades, and I know Edmunds likes to help to give people information. What are the kinds of data points that people should have with them, whether it's their mobile device, or perhaps printed out, or even actually in their mind, when they are going to talk to a dealer? What kind of advice can we give them?
Jeremy Acevedo: Certainly one of the first things you want to look at is the actual list price of a car, and what cars are selling for in your area, which is available on Edmunds through the TMV program. So, we have a price that consumers are actually looking at that shows what cars are actually transacting at, and that's certainly a fair market price, and we encourage our users to use that number.
Mark Hamrick: What does TMV stand for?
Jeremy Acevedo: True Market Value. That is the true market value of vehicles and so that's a great starting point for negotiations for consumers.
Mark Hamrick: So that seems like that's really the bottom-line number that people need to be focused on.
Jeremy Acevedo: Exactly, and so having a number like that in mind is certainly very helpful. Additionally, you can research the incentives on, and not just our website, but any online source, incentives are made available so you can actually look at advertised leases like you've seen, and advertised finance deals as well.
Mark Hamrick: OK, that's great information there, Jeremy, and an exciting time to be in the market for a car, and perhaps even to be driving one, but we really appreciate you helping to break it all down for us.
Jeremy Acevedo: Absolutely, Mark. It was a pleasure.
Mark Hamrick: Jeremy Acevedo, an auto analyst with Edmunds.com, he spoke with us from his office in Santa Monica, Calif.
And don't forget to check out Bankrate.com for all kinds of useful information on auto ownership, including loan rates, insurance quotes and calculators. Just look for the auto tab at the top of the page.
There's no shortage of horror stories around about high levels of student loan debt. Bankrate's associate editor, Doug Whiteman, tells us there are other options, though, involving education, particularly for workers whose employers will foot the bill for their training.
Doug Whiteman: Working by day and hitting the books at night is a difficult balancing act, but your employer might help you advance your education by offering training that earns you college credits. Courses such as Starbucks' Barrista Basics, and a program offered to Jiffy Lube employees, allow workers to build skills they can use on the job, while also putting them on a path toward a college degree. Starbucks has a relationship in its hometown of Seattle with City University, which provides college credit for the company's training programs, and offers Starbucks employees a break on tuition and a waiver of the school's usual application fee.
One expert says a company once faced a backlash from a union that claimed one of these college training programs was being used to try and nonunion replacement workers in the event of a strike. But she also says affiliations between big business and higher education help improve access to higher learning for working adults. For "Your Money This Week," I'm Doug Whiteman.
Mark Hamrick: Next up, interest rates and the Federal Reserve; this is the bread and butter of what we cover here at Bankrate, guiding so much of our daily financial lives. For our next segment, we spoke with economist David Nice of Mesirow Financial. As we head into the autumn months, I asked him where the U.S. economy stands at the moment.
David Nice: The economy, it's not growing as fast as you would like it this far after a recession, but recent data in the housing market, new-home sales, took a major hit, but on the opposing side of that, existing-home sales did quite well. Durable goods came in very poorly, so I feel as though the economy is fighting for direction right now.
Mark Hamrick: OK, does that mean it's in risk of truly faltering to the point where we could even have a recession? Or it's really a case of 2 percent growth versus 1 percent or less?
David Nice: I don't think we are there yet. I think the subpar growth might stick around for longer than we'd like. I don't really see us as being at major risk for a proper recession yet, but like I said, growth is not as robust as you would like to see right now.
Mark Hamrick: Right, and generally, of course, we gauge things by how consumers are fairing and how workers, or prospective workers, are doing; and progress on both of those fronts has also been less than generally seen in a recovery. Is there any reason for hope there?
David Nice: There's always hope, but the numbers that have come in for wages have been slack for months and I don't really see that changing just yet. The stagnation that you see in wages is kind of putting a damper on people's disposable income and how much they can actually spend.
Mark Hamrick: Obviously, there's a link between what workers are doing and their ability to spend as consumers. Let's talk a little bit about interest rates. Those controlled by the Federal Reserve have remained at record-low levels for years now, but market-based interest rates have been rising this year. Should people be bracing themselves for further increases in interest rates?
David Nice: I think we will have more information on that after the Fed meeting this month, but I believe rates have plateaued. I think the market is priced at taper in September at this upcoming meeting, with regard to the 10-year and the 30-year mortgage; I think both have reacted to the perception that the Fed will start tapering this month.
Mark Hamrick: OK, so tapering involves this process of asset purchases that the Federal Reserve has been engaged in. Three rounds of so-called quantitative easing, and we know the Fed really wants to, if it can, sort of back out of that. So, you're referencing there the fact that the financial markets have been essentially discounting that, or anticipating that, and that gets to the notion that the Fed has been doing these extraordinary things since this extraordinary flame out for the economy. Of course, it was the housing market where problems have snowballed into the full-fledged financial crisis. With rates at least higher, will housing falter?
David Nice: I don't think it will falter, but I think you will see a bend in price appreciation downward. I think the housing market is on stable ground, and I think that the rising rates will obviously put a damper on how much of a house people can buy, but I still see the housing market growing pricewise.
Mark Hamrick: So in other words, we will see price increases for homes, but not quite at the rate that we have seen before.
David Nice: Exactly.
Mark Hamrick: OK, so there's been a lot of discussion lately about who heads the Federal Reserve. We know that Chairman Ben Bernanke is slated to leave at the end of January. It's President (Barack) Obama's decision to appoint someone, and there's been all kinds of discussion whether that might be the current vice chair, Janet Yellen. Larry Summers is somebody who seems to be gaining some favor. He's a former treasury secretary in the Clinton White House. From where you sit, how much does it matter who is the chairman of the Federal Reserve?
David Nice: It would depend most on consistency and the ability for the (Federal Open Market Committee) as a whole to be able to convey their message to the markets. If either candidate could do so, I think either selection would be fine.
Mark Hamrick: And obviously the president has to make his decision, and then the Senate needs to go along, so there's obviously a lot to watch there. David, when consumers or borrowers or investors are watching the activity and whether it's the markets or the economy, what do you think they are most at risk of sometimes missing? Maybe a disconnect between what economists see, and what the average person sees.
David Nice: With regard to interest rates, I would say consumers may miss how quickly markets could react to such activity. If you were in the market for a home back at the beginning of the year where you were able to get a rate as low as 3.4 percent, you might expect that rate to stay there for a longer period of time than it has. Rates have jumped up in the past few months over a 100 basis points, so I think the quickness in which interest rates could change, I think could sometimes surprise a consumer.
Mark Hamrick: Well, we certainly saw the markets react quickly during the financial crisis in a largely negative way and obviously, they can move the other way as well. David, we really appreciate your time. Thanks so much.
David Nice: Thank you.
Mark Hamrick: David Nice, associate economist from Mesirow Financial; he spoke with us from his office in Chicago.
How do you measure your reputation? Well, if we are talking about your financial track record, one gauge is your credit score. If you don't know about the information on your own credit report, it is worth looking into. Bankrate's credit card analyst, Janna Herron, knew that to be the case, but also recently was given a dramatic reminder herself.
Janna Herron: Do you know how much a collections account can hurt your credit score? Up to 100 points, FICO says. But I can tell you from personal experience, that the damage can be even worse, to the tune of a 150 points. Last year I received a credit score disclosure after I didn't qualify for the best interest rate on a credit card. My score, a lousy 651. To get the best rates on most loans, you need a score in the mid-700s. I had thought my score would have been higher because two old delinquencies had finally fallen off my credit report after seven years, and those were the only negative items still on my report. Or so I thought.
After pulling my credit reports, I found the culprit, a disputed dental bill that had gone into collections. It was time to bring out the big guns and settle the dispute. I wrote letters to the Better Business Bureau and the state attorney general's office pleading my case. I sent copies to the collection agency and dental office. Fast forward two months, and the collection item disappeared. The collection agency confirmed that the claim had been recalled in error. I had won; but what was my prize?
Well, last month, a mortgage lender pulled my credit score again. The result? 805. Lessons learned? Always get your free credit report. Every year, AnnualCreditReport.com so you're never blindsided by what's in it; and always put up a fight if you find an error. Take it from me; it will save you money. I'm Janna Herron, for "Your Money This Week."
Mark Hamrick: We began talking about cars and we'll end there, too, with a story about a dramatic ending.
It's our look at this week in business history. On Sept. 14, 1899, the U.S recorded its first known fatality involving an automobile. The victim was a man named Henry H. Bliss, who worked in real estate. The 13th of the month was unlucky for him, when he got off a New York City streetcar near Central Park West and was hit by a taxicab. According to The New York Times, Bliss was knocked to the ground and the car crushed him, and he died the next day. Pedestrians were to develop a greater sense of urgency, looking out for cars when crossing the road, as automobile traffic picked up in the 20th century.
You've been listening to "Your Money This Week." If you enjoyed the podcast please rate and subscribe to our program. We're hoping that you can help us to get the word out. For more on these and other personal finance issues, visit Bankrate.com, and you can follow us on Twitter @Bankrate. Our editor-in-chief is Julie Bandy; managing editor, Katie Doyle; assistant managing editor, Holden Lewis. And thanks to producer Lucas Wysocki for his work back in the studio. I'm Mark Hamrick. From all of us here at Bankrate, here's hoping you have a great week.


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Posted 5 years ago
(53 items)
Boxed set of three pop-up paper Christmas ornaments designed by Robert Sabuda. Made by MoMA for Borders Books.
Robert Sabuda is a New York Times best-selling creator of children's books. He is celebrated for his innovative and elaborate pop-up books. These were produced exclusively for Borders Books, the now defunct, very stylish American bookstore.


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She's a pearl!
My grandmother would always say "She's a pearl" or "He's full of pearls" meaning they were wise or full of wisdom. Those folks were also her favorites and always had her laughing. Pearls, the birthstone for June, have a special place in the gem world because they arrive at the jewelers bench organic and already perfect in their own right. All other gems and minerals have to be cut, refined, heat enhanced, shaped, etc. Pearls come from their shell ready for the world. They are also the only gem I can think of that is a noun, verb, adjective, and even qualifies as for the tag idiom, courtesy of the Bible.
Pearl: noun 1. A smooth, rounded bead formed within the shells of certain mollusks and composed of the mineral
aragonite or calcite in a matrix, deposited in concentric layers as a protective coating around an irritating foreign
object: valued as a gem when lustrous and finely colored. Example: Pyramid Studios has beautiful pearl jewelry. 2. A girls name……although, here in Maine, I have known a couple men to be named Pearly. 3. A very pale grey color. Verb 4. To dive, fish or search for pearls. Adjective 5. Resembling a pearl in form or color; having or reduced to small grains. Idiom 6. "Casting pearls before swine" meaning to offer something of great value to those incapable of appreciating it. As in "The teacher read the class Shakespeare but she was casting pearls before swine." Pearls, the noun, are a specialty at Pyramid Studios! Shop all our wonderful Pearl Selections!
Shop Pyramid Studios Pearl Collection Here


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The Top Ten Most Popular Recipe Collections from Two Years of Slow Cooker from Scratch!
Two years ago today I officially launched Slow Cooker from Scratch, starting the adventure of finding and featuring from-scratch slow cooker recipes from talented food bloggers all around the web. When I was thinking about an appropriate post to celebrate the two year birthday, I thought about sharing the top ten recipes from the last year. Then I checked my stats and I was surprised to see that many of the most popular posts this year have been round-ups. (I was delighted to see that, because one reason I started doing more round-up posts was to have a chance to feature more blogs.)
So to celebrate two years of slow cooker goodness I decided to share The Top Ten Recipe Collections from Two Years of Slow Cooker from Scratch! I hope you find at least a couple of new recipes to try.


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Summary: Two bhikkhus questioning one another on the Abhidhamma would illuminate the Gosinga Grove."Idhāvuso Sāriputta, dve bhikkhū abhidhammakathaṃ kathenti, te aññamaññaṃ pañhaṃ pucchanti, aññamaññassa pañhaṃ puṭṭhā vissajjenti, no ca saṃsādenti, dhammī ca nesaṃ kathā pavattinī hoti. Evarūpena kho, āvuso Sāriputta, bhikkhunā gosiṅgasālavanaṃ sobheyyā"ti.
Of course, Abhidhamma is mentioned many times in the Sutta Piṭaka, e.g. in the Mahāgosinga Sutta of the Majjhimanikāya.
Some people just like controversy. It is better to steer clear of it
and get on with one's own study and practice.
Adrien wrote:but that the story of how the abhidhamma was taught is to be found only in commentarial texts.
Adrien wrote:Actually he didn't sayed that he could cure aids, but that he knew how to heal himself from aids if he happens to get it (which is still a little strange).
However, this is not the subject here. Being wrong/foolish at one time doesn't mean being wrong/foolish all the time. And there is no need to value Bhante Vimalaramsi's credibility here, since he is only talking about things we can easily verify.
So if you disagree whith what he says in this video (which I assume you do), please offer us some counter arguments. Please consider the words, not the speaker, and explain why you disagree whith him. I would be interested in your answer if you had the time and motivation to do it. And I'm not saying this in a challenging way, I would be truely interested in what you have to say
retrofuturist wrote:Adrien wrote:but that the story of how the abhidhamma was taught is to be found only in commentarial texts.
... and that the story does not accord with the Buddha's own words that he did not teach with a closed fist (i.e. no secretive teachings given only to exclusive groups)
retrofuturist wrote:Greetings,mikenz66 wrote:That Ven Vimalaramsi was thoroughly disillusioned by his experiences in Burma is actually quite relevant because his talks (and I've listened to over 100 of them, since they are mostly based on reading Majjhima Nikaya suttas and commenting on them) are clearly shaped by that.
Well, we have had a thread on this guy earlier on. He is no more attractive now than he was then. I'll look elsewhere for my Dhamma teachings.retrofuturist wrote:Greetings,
An interesting video from venerable Vimalaramsi on..
How the Visuddhi Magga and the Abhidhamma has influenced the Buddha's Teachings
It goes some way to explaining why some people may legitimately prefer a suttanta method of meditation over that of one based upon post-Buddha teachings. Frankly, I find it refreshing to see a monk have the courage to defend the primacy of the Buddha's teaching, against the traditional backdrop of accumulated post-Buddha perspectives.
tiltbillings wrote:Well, we have had a thread on this guy earlier on. He no more attractive now than he was then. I'll look elsewhere for my Dhamma teraching.


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The RBS Six Nations 2014 championship starts next weekend and the six team captains met up in Putney this morning to officially launch the tournament.
England skipper Chris Robshaw was joined by fellow captains Sam Warburton, from Wales, Paul O'Connell, from Ireland, Scotland's Kelly Brown, Italy's Sergio Parisse and Pascal Pape from France.
England begin the tournament with tough trips to Paris and Edinburgh, but face Ireland and then Wales at Twickenham before finishing the tournament by heading to Rome where Italy will lie in wait.
Robshaw said: "We've got two away games to start, so it's going to be challenging but we've got to make sure we're up to speed and hit the ground running."
British Lions captain Warburton confirmed he will be available to lead his side, after overcoming injury, while Les Bleus skipper Pape had the press conference in hysterics when he apologised for his poor English, but instead used a much stronger word than "poor".
But Scotland captain, and stammer sufferer, Brown stole the show when he mocked the "smooth delivery" of his fellow captains before soldiering on through a tricky Q&A.
The respective coaches from the six camps were also at the event, at the Hurlingham Club next to Putney Bridge station, ahead of the start of the competition on February 1.
Wales are the reigning champions having won the Six Nations on the final day of the season, they also won the Grand Slam in 2012.


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Brett's return to Royals dugout surprises Gardenhire
KANSAS CITY -- Count Twins manager Ron Gardenhire among those surprised by Hall of Famer George Brett's decision to put on the uniform again as interim hitting coach for the Royals.
While the Twins relaxed in their clubhouse before batting practice Tuesday, Brett held a press conference down the hallway at Kauffman Stadium prior to his first home appearance in the new role.
"To have a guy of his stature, a Hall of Famer, want to go down there and see if he can help some people ... that's pretty cool," Gardenhire said.
Gardenhire played five seasons in the National League with the Mets and didn't cross paths with Brett, who spent his the entirety of his 21-year career with the Royals.
"I cheered him on from afar," Gardenhire said. "I always loved the way he played."
Robert Falkoff is a contributor to MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.


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Robertson and Delsing re-elected chair, vice chair of NWB
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Von Johnson of Cambridge was recently elected chairman of the Nebraska Wheat Board. Rick Larson of Potter was elected vice chairman.
Johnson is in his second term on the Nebraska Wheat Board of Directors and has served since 2005. A representative of District 5, Von and his wife Becky live and farm in Furnas County.
They raise certified wheat and soybeans, as well as corn and alfalfa. Johnson also serves on the Food Aid Committee for US Wheat Associates and is a member of the Nebraska Wheat Growers Association.
Larson began serving on the NWB Board of Directors in 2003. He represents District 2 and farms in Banner and Kimball Counties. Larson and his wife Diane farm irrigated and dryland wheat, corn edible beans and alfalfa. Larson also serves on the Hard White Wheat Committee for US Wheat Associates and is a member of the Nebraska Wheat Growers Association.
Both Von and Rick are excellent wheat producers who are dedicated to the success of the wheat industry in this state. The future of the Wheat Board is bright with these two at the helm, said Larry Flohr, a NWB director and past chairman.
Members of the board of directors are appointed by the governor to serve five-year terms. The chairman and vice chairman are elected by the board and traditionally serve two years in that position.
The Nebraska Wheat Board administers the one and one quarter cent per bushel excise tax levied on wheat markets in Nebraska. Beginning October 1, 2012, the board will administer an excise tax of 0.4% of net value of wheat marketed in Nebraska at the point of first sale.
The board invests the funds in programs of international and domestic market development and improvement, policy development, research, promotion, and education.
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