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Electronic Security Apprenticeship DPE Systems Ltd
This vacancy is for a Security Apprenticeship, based in Bolton, Greater Manchester. The successful applicant will have the opportunity to undertake valuable work experience whilst gaining on the job qualifications.
This apprenticeship requires you to apply through the employer's website.Apply now
Closing date: 29 Sep 2017
Apprenticeship summary
Weekly wage
Wages explained
The current National Minimum Wage for an apprentice is £3.50 an hour. This rate applies to apprentices under 19 and those aged 19 or over who are in their first year.
Apprentices must be paid at least the minimum wage rate for their age if they are aged 19 or over and have completed their first year.
The minimum wage rates are £5.60 an hour for 18-20 year olds and £7.05 for anyone aged 21 and over.
Apprentices are paid for their normal working hours and training that's part of their apprenticeship (usually one day per week).
Working week
37.5 hours p/w TBC at interview
Total hours per week: 37.50
Apprenticeship duration
4 years
Possible start date
09 Oct 2017
Date posted
08 Jun 2017
Apprenticeship level
Advanced Level Apprenticeship
Reference number
2 available
The vacancy is an opportunity for someone who is interested in pursuing a career in Engineering. If successful, the apprentice will be based at their employer working the hours agreed in their contract. The apprentice will need to attend Training 2000 to complete the relevant skill units that have been agreed by Training 2000 and the employer, this will consist of the apprentice completing 3 blocks of skill specific Engineering training lasting 4 weeks each. As well as this they will also begin their Further Education course, BTEC Diploma, City and Guilds, which will be 1 day a week classroom based learning.
The Providing Electronic Security and Emergency Systems qualifications are ideal for engineers and others working within the electronic security and emergency systems industry. They have been designed to meet the needs of engineers involved with security and emergency systems, installation, commissioning, maintenance, auditing, surveying and design.
Fire, Emergency and Security Systems Technicians design, install, commission and maintain electronic systems in and outside simple and complex premises to protect individuals, homes and properties from risk and danger.
Systems include fire, security and emergency systems to detect intrusion, provide surveillance, monitor and control access to buildings, properties and sites or to detect fire and emergencies.
Skills include interconnection of equipment, programming, verifying performance/fault finding and testing and maintaining.
Technicians will carry out planned jobs to install new systems, modify and maintain existing systems as well as respond to call-outs to repair faulty systems where they will utilise their problem solving skills.
They will take a professional approach to customer service skills which include being presentable, tidy and respectful as they can often find themselves working in and outside customers' homes as well as in and outside business premises.
It is important for Fire, Emergency and Security Systems Technicians to be able to work independently or as part of a team and use their knowledge and skills to ensure systems have been appropriately selected and installed and maintained to a professional industry standards, often without any supervision, and done so in a safe, efficient and economical manner to minimise waste.
Requirements and prospects
Desired skills
Good attention to detail
A technical mind
Ability to use initiative to think through problems and find solutions
Ability to work as part of a team
Excellent communication skills
The ability to think outside the box, curious and innovative
Personal qualities
Must possess an interest in engineering
Motivated and committed individual
Good time keeping
Strong work ethic
Qualifications required
Minimum of 5 GCSEs grade C or above including English, Maths. Science and Technology is desirable. Other equivalent qualifications are acceptable.
Future prospects
About the employer
Training provider
Technical Certificate, Level 3 e.g. City and Guilds 2800 or BTEC Diploma
Functional Skills, Level 2 in Mathematics, English, ICT, working with others and improving your own learning. Functional Skills requirements is subject to the successful applicants GCSE results. Grade A-C and within 5 years old means the apprentice will be relaxed from completing functional skills. Certification evidence will need to be provided.
Employer Rights & Responsibilities
SEMTA Apprenticeship in Engineering Framework (Once all elements are completed and learners portfolio has passed Internal Verification)
Level 3 Qualification in Engineering
1 week (Monday –Friday) in-centre training at Training 2000's Blackburn facility approx. every 8 weeks for 3 years.
On-site visits from a Training 2000 Training Officer approx. every 6-8 weeks
Apprenticeship framework
Engineering Manufacture
Training provider
Applications for this apprenticeship are being processed by Training 2000 Ltd
Chris Cumpstey 01254 54659
Employer's Application Instructions
Please can you apply online at http://www.training2000.co.uk quoting the reference number of the vacancy you are interested in. If you have already applied online please call 01254 546 59 and we can add the vacancy to your current application form.
This apprenticeship requires you to apply through the employer's website.Apply now
Closing date: 29 Sep 2017


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Intelligence: The Secret World of Spies: An Anthology by Professor of National Security Affairs James J Wirtz, Professor of Political Science Loch K Johnson (Paperback / softback, 2014)
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- DescriptionIntelligence: The Secret World of Spies--An Anthology, Fourth Edition, is the most up-to-date reader in intelligence studies. Editors Loch K. Johnson and James J. Wirtz present a diverse, comprehensive, and highly accessible set of forty readings by leading experts in the field. This unique volume features comprehensive coverage of many hot topics including methods of intelligence collection, intelligence analysis, the danger of intelligence politicization, relationships between intelligence officers and the policymakers they serve, covert action, counterintelligence, accountability and civil liberties, the implications of major intelligence failures in 2001 and 2002, and recent controversies over highly classified documents leaked by insiders.
- Author BiographyLoch K. Johnson is Regents Professor of Public and International Affairs at the University of Georgia. He is the author of more than 200 articles and the author or editor of twenty-eight books. James J. Wirtz is Dean of the School of International Graduate Studies at the Naval Postgraduate School and Director of the Global Center for Security Cooperation, Defense Security Cooperation Agency.
Key Features
- Author(s)Professor of National Security Affairs James J Wirtz,Professor of Political Science Loch K Johnson
- PublisherOxford University Press Inc
- Date of Publication15/07/2014
- LanguageEnglish
- FormatPaperback / softback
- SubjectCurrent Affairs & Issues
Publication Data
- Place of PublicationNew York
- Country of PublicationUnited States
- ImprintOxford University Press Inc
- Weight1225 g
- Width203 mm
- Height249 mm
- Spine28 mm
Editorial Details
- Format DetailsTrade paperback (US),Unsewn / adhesive bound
- Edition Statement4th
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- 6
How to Rodent Proof Your Attic
It's 2 am; do you know what that pitter patter sound is coming from your ceiling? Did you know that mouse contaminates are recirculated into the air you breathe?Learn how spot a rodent infestation.
- 3
Best Mouse Trap for a Lost Pet Mouse
Petunia my pet mouse escaped during an earthquake and was gone for days. Traveling through a maze of mouse traps, she safely made her way to the most humane trap of all. She had to make a choice, for she was hungry,...
- 12
Best Mouse Traps: Kids and Pet Safe Solution
Searching for the best mouse trap is useless unless you intend to do a little research first. How many traps you need depend on how many mice you have. How can you know that number? Are you sure your squatters are...


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Magic Moments is a singer-songwriter, producer and video artist hailing from Los Angeles, California. His aim is to spread positivity and inspiration through his lyrics and music, which incorporate elements of indietronica, emotive vocal synthpop, freestyle hip-hop and dance.
Reference Links For This Wiki
All information for Magic Moments's wiki comes from the below links. Any source is valid, including Twitter, Facebook, Instagram, and LinkedIn. Pictures, videos, biodata, and files relating to Magic Moments are also acceptable encyclopedic sources.
0http://magicmoments143.com/PhoenixOfficial homepage
Article Discussion (0)
This is a discussion about Magic Moments. Concerns about the topic, its accuracy, inclusion of information etc. should be discussed here. Off-topic discussion not pertaining to Magic Moments or this wiki will be removed.
See Also
Created: March 8, 2016, 3:07 a.m.
Last Modified: June 11, 2016, 6:36 p.m.


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The Food & Beverage Director is responsible for planning, organizing, developing and directing the overall operation of the Food Service Department in accordance with State Regulation and Five Star Policies and Procedures to ensure that quality food service is provided at all times.
Educational preparation at a level appropriate to assure fulfillment of essential duties. Experience and/or training in nutrition, food preparation, safe food handling techniques and food service management required. Prefer two (2) years of food service supervisory experience in a restaurant setting focusing on food management, cost control and presentation. Computer skills are necessary for food ordering, email, and report development/tracking.


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Jobs in Wigan, Greater Manchester (GB)
To get your perfect job in Wigan, Greater Manchester (GB) sent to you the day it's posted, sign up for our job alert service.
Found 6 jobs in this campaign
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Regular Surgery Sessions available in and around Greater Manchester!
Various long term / regular surgery sessions available in Oldham, Wigan, Bolton areas of Manchester, and other surrounding areas. Emis Web - 09.00...
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A surgery with big potential, based within a progressive CCG area. Reasonable patient numbers and first-rate support.
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GP required for surgery in Wigan
Locum GP required to work in surgery in Wigan ,they work 4 hour am sessions 2pm - 6pm and 4 hour am sessions 9am - 1pm We won't pay you a daily r...


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List all words that contain fados
Only the word fados was found, nothing longer.
Some random words: kifs haslet eparch imipramine khaddar juba fyce
This is not a dictionary, it's a word game wordfinder.
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|Home / CD, DVD Ripper / Cheersc MP3 WAV Converter
|home | contact us | submit | help
* Cheersc MP3 WAV Converter is a super audio converter to convert MP3 to WAV or WAV to MP3. It supports decoding audio formats like WMA, AU, AIFF, M4A, M4A, APE, OGG, AAC, MP2, VQF, FLAC and encoding them to MP3 or WAV. It also can extract audio from video file formats such as AVI, WMV, MPEG, ASF and MP4, etc. * Features: 1. It is easier to use and faster than ever - With the concise interface and design, users can easily handle the software without any help. The converting speed is improved in Cheersc MP3 WAV Converter so users don't have to wait so long. A file can be converted within just a few seconds. 2. It supports ID3 tag - All ID3 tags in the original files will be reserved after the conversion. 3. Codes are built-in - The software integrates with all audio encoders and decoders - users can convert all supported audio formats once download the program. 4. It supports batch conversion - You can select all files you want to convert and Cheersc MP3 WAV Converter will automatically encode them one by one. 5. The user can choose output path - You are able to edit the output path or use the same one as the input path. 6. It offers excellent output quality - Using Cheersc MP3 WAV Converter, you will get any output formats you want with excellent quality. 7. Support Windows Vista RTM. * It offers excellent after service that all users' emails will be replied within a business day! Come on to try it now - download is FREE! * Great news: 1. the new version of Cheersc PSP Video Converter will support h264 conversion! 2. the new version of Cheersc MPEG Encoder will support h264 conversion and offer wizard mode to the beginners as well as advanced mode to the experienced! Cheersc Inc is one of the pioneers in DVD, video and audio software development.We endeavor to develop most easy-to-use, steady and detail-focused software products.
home | contact us | submit | help | development
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New printable coupons are available for Nature's Bounty Vitamins. Use the "Magic Coupon Machine" at CVS with store coupon from manufacturer and a BOGO sale this week, and score Nature's Bounty vitamins for as low as a quarter.
Buy 2 Nature's Bounty Potassium Gluconate 595 mg Caplets, 100 ct $5.49-$6.29, regular price
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Nature's Bounty Vitamins Coupon Sep 2013, Save 2013


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You turn to Democracy Now! for ad-free news you can trust. Maybe you come for our daily headlines, or for in-depth stories that expose government and corporate abuses of power. Democracy Now! produces our daily news hour at a fraction of the budget of a commercial news operation, all without ads, paywalls, or government and corporate funding. How? Only with your support. If you and every website visitor this week gave just $8/month, it would cover our basic operating costs for the entire year. Right now, a generous donor will double your new monthly donation to Democracy Now! Pretty exciting, right? So, if you've been waiting to start your monthly gift to Democracy Now!, today is your day. If you visit us daily or weekly or even just once a month, please do your part today.
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Coordinator of Tasaru Ntomonok Initiative in Kenya, a community-based group to save girls from female genital mutilation and early marriages. In 2002, she helped V-Day open a safe house in Narok, Kenya to create a safe haven for young girls seeking refuge from female genital mutilation (FGM) and early childhood marriage.
We rely on contributions from our viewers and listeners to do our work.
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JULY 2nd - 7th 2018
Venue: Deerhurst Resort
Entry Deadline: Saturday, March 31st, 2018
TITLE SOLOIST ENTRY DEADLINE: June 1st, 2018
*Title Soloists must have received a solo overall at one of our 2018 regional events to qualify. Additionally, if you received a Greece Intensive in 2017 or 2018 you are also qualified to compete in our Title Competition.
Deerhurst Resort
235 Deerhurst Drive
Huntsville, Ontario
Parking: Complimentary
Air Canada Discount Booking Code: TBD
Host Hotel: Deerhurst Resort
Deerhurst Resort
235 Deerhurst Drive
Huntsville, Ontario
*All reservations must be made by phone in order to receive the preferred rate
*Please mention VIEW Dance Challenge to receive the discount
Pavilion Guest Rooms: $199.00/Night
Lakeside Guest Rooms: $199.00/Night
One Bedroom Suites: $219.00/Night
One Bedroom Condos: $259.00/Night
Two Bedroom Condos: $479.00/Night
Three Bedroom Condos: $679.00/Night
Large One Bedroom Condo: $279.00/Night
Large Two Bedroom Condo: $529.00/Night
Large Three Bedroom Condo: $709.00/Night
Check In: Monday July 2nd, 2018
Check Out: Sunday July 8th, 2018
Cut Off Date: Friday June 3rd, 2018
Amenities: Complimentary Wifi, Parking, Indoor Pool and Fitness Facility


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67 маршрут ульяновск This правила проведения комплексной BRAND IDENTITY KIT FOR G STATION is a WIP (Work in Progress) it has recently been accepted by my newest client. My client doesn't mind me sharing because it gives exposure so, here it is!американская история ужасов 1 скачать торрент
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как делать микроблейдинг бровей поэтапно I'll add the website to show the finished project. Wish me luck!ручной мяч правила


text: June 24, 2017 HomeAsia / PacificOver 100 people feared buried in China landslide Over 100 people feared buried in China landslide By admin Asia / Pacific 0 Comments Your account has been deactivated. Related Posts Pakistani Taliban launch women's magazine for would-be jihadists Malaysia charges two women with Kim Jong-Nam murder Palestinian Authority bans novel About Author admin Add a Comment Cancel reply Your email address will not be published. Required fields are marked *Comment:*Name:* Email Address:* Website:


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IBIS Delhi Airportasset no 9 hospitality , district delhi aerocity , igi airport , 110037 - new delhi Delhi India 110037View on Map
- Parking Facilities Available
- Internet Access
- Swimming Pool
- Fax Machine
- Wheelchair Accessibility - Room
- Car Rental
- Business Center
- Pets Allowed
- Conference Facility
- Laundry Service Available
- Health Club / Gym Facility Available
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Good hotel with nice services but no food after 12 only French fries
Room was too small compared to the price. The most irritating thing was paying the food bill for every order. They should add the bills to be paid at the check out time. Food Menu was not that great.+ More
Excellent location. room iis cramped. basic amenities like newpaper / toileteries also not provided. breakfast quality is average. overall, not so good experience in terms of value for money propositi+ More
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Our peaceful cottage on Lake Huron will provide a wonderful place for a couple's retreat or a family vacation. With three bedrooms and a pull out couch, there is plenty of room to accommodate a family with children. There is a large deck overlooking the beach for dinners and cocktails. The entire cottage has been renovated with a new kitchen and bathroom. Our gourmet kitchen is fully stocked and is baker friendly perfect for brunch. Enjoy the lake with Kayak and paddle board. Golf and bike paths are nearby. Come and enjoy the beautiful sunrise.
No smoking
No pets
Be mindful of the wood floors and rugs.
Clean feet of sand before entering home.
Cancela hasta 5 días antes de tu viaje y recibe un reembolso completo. Cancela en los 5 días previos a tu viaje y no se te reembolsará la primera noche, pero sí el 50 % del costo del resto de las noches.
14 evaluaciones
Hello! I have been visiting this beautiful beach since 1992. I live in California and I had to have my own piece of paradise in Michigan. My cottage was renovated in 2016 and is ready for others to enjoy!


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The Fashion Industry Has Long Been One Of The Largest Employers In The United States, 24 And It Remains So In The 21st Century.
Technological influences are growing more apparent in the fashion industry. After all, what better time to cut your mane than the summer? Social media is changing the way practitioners deliver messages, 11 as they are concerned with the media, and also customer relationship building. 45 BR practitioners must provide effective communication among all platforms, in order to engage fashion public in an industry socially connected via on-line shopping. 46 Consumers have the ability to share their purchases on their personal social media pages such as Facebook, Twitter, Instagram, etc., and if practitioners deliver the brand message effectively and meet the needs of its public, word-of-mouth publicity will be generated and potentially provide a wide reach for the designer and their products. For example, an American fashion company might source fabric in China and have the clothes manufactured in Vietnam, finished in Italy, and shipped to a warehouse in the United States for distribution to retail outlets internationally. Fashion is not only seen as pure aesthetic values; fashion is also a medium for performers to create an overall atmosphere and express their opinions altogether through music video. One of the biggest silhouettes... 2015 Fall & Winter 2016 Haircut Trends. Show & Launch of the Latest Collaboration Capsule Collection Puma and Trapstar Previewed the Autumn/Winter... This year in particular shorter hair has been the preferred hairstyle choice among celebrities and designers making it the true "IT"... 2015 Fall / Winter 2016 Hair Trends.
Baird & Company, Inc. - Analyst [10] -------------------------------------------------------------------------------- Okay. And then, I guess, just a last question for you, Jim. -------------------------------------------------------------------------------- Jim McDonald, Rocky Brands, Inc. - EVP, CFO, and Treasurer [11] -------------------------------------------------------------------------------- I think, Mitch, one of the things, it took us -- we finally got that operation leaned out in the fourth quarter. And that's made it possible for us to be profitable at lower levels of sales, just as our other operations are. So we're in good shape there. And that was one of the reasons we were able to make a profit in the fourth quarter. -------------------------------------------------------------------------------- Mitch Kummetz, Robert W.
Time to wipe away that dull polish, and brush on new vibrant hues for the spring and summer 2016 seasons! Internet technology such as on-line retailers and social media platforms have given way for trends to be identified, marketed and sold immediately. In recent years, fashion bogging and YouTube videos have become a major outlet for spreading trends and fashion tips, creating an on-line culture of sharing one's style on a website or Instagram account. With bogging platforms such as Instagram, tumble, Wordpress, and other sharing sites, floggers have emerged as expert fashion commentators, shaping brands and having a great impact on what is 'on trend'. 44 Women in the fashion public relations' industry such as Sweaty Betty BR founder Roby Jacenko and Oscar de la Rena's BR girl Erika Berman, have acquired copious amounts of followers on their social media sites, by providing a brand identity and a behind the scenes look into the companies they work for. Bold Hair colon Ideas To Inspire Your Next Dye Job. For those of you who are curious about fall's hottest hairstyles, your in for quite the retro throwback. Music is a representation of fashion that expresses the abstract design concept into relatable harmony for viewers. This is ideal for those who don't want to commit to a full head of colon or for those looking to make a subtle yet unique hair change.


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Platforms: PC, PS3, XBOX
Genre: Action
Publisher: CapcomDeveloper: Capcom
Esrb rating: Teen (13+)
Released: March 6, 2012
Official site: Streetfighter.com/us/sfxtk
About: Street Fighter X Tekken is a crossover fighting game. The game features characters from both the Street Fighter franchise and Namco's Tekken series. The center of the conflict between the Street Fighter and Tekken universes is a cubical object that crash-lands in Antartica. The object is of a mysterious origin and researchers worldwide are unable to determine what purpose it serves. The game features two new game mechanics: the Gem System and Pandora Mode.
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Tomb Raider is an upcoming open world platform action-adventure game.
DmC: Devil May Cry is a hack and slash beat 'em up game. The game is set in a parallel universe in the Devil May Cry series. It starts with Dante recovering from a hangover when he is attacked by a Hunter demon. Dante learns he is in Limbo City, a dreary city that appears calm at firs...
Assassin's Creed III is an upcoming historical action-adventure open world stealth game. The game will be set during the American Revolution from 1753 to 1783 and will feature a new protagonist who calls himself Connor. Connor Kenway is a young half-English, half-Native American man. ...
Borderlands 2 is a science fiction–based first-person shooter game with role-playing-elements. It is sequel to Borderlands. At the start of the game, players select one of four new characters, each with a unique special skill and with proficiencies with certain weapons. The game beg...
Sleeping Dogs (originally True Crime: Hong Kong) is an action open world crime drama game. Sleeping Dogs focuses on a police officer attempting to bring down criminal factions. The game takes place in Hong Kong with players assuming control of Detective Wei Shen, an officer of the Tri...


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Hire our best Music Producers in Munster, DE
Get quality creative freelancers to help you grow on Twine
Music Producers for hire in Munster, DE
We have over 200,000 creative freelancers! Twine will help you find the perfect Music Producer for your project.
"Music is my Therapy"! Nach diesem Motto verwöhnt DJ Avalix sein Publikum mit den feinsten Beats. Mit 14 Jahren wurde er mit der Musik vertraut gemacht und mit 17 Jahren fing er an seine ersten MashUp's und Remixe zu produzieren. Einige Jahre später bekam er sein ersten Auftritt bei seinem ersten Club, wo er durch DJ Q unterstützt wurde. Nach einem erfolgreichen Abend war er dann nicht mehr zu stoppen und legte auf Geburtstagen sowie auf Abschlussfeiern auf. Mit 19 bekam er dann sein ersten offizielen Auftritt in der Diskothek 4400. Referenzen: Club 4400 (Heimatliebe)
Our Music Producers have 1 ratings with an average of 4.1/5See more freelancers
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Latest music producers posts from the blog:
Spotlight: Ian Sean
Ian Sean is a rapper from New York who's been building up a reputation for himself on Twine by collaborating with music producers all over the world. In fact, we recently featured one of his pro...Read more
Make the reverb of your dreams
Ever wondered what your tracks would sound like recorded in a trash can, on a bus or in Wembley Stadium? Welcome to the wonderful world of convolution reverb! What is convolution reverb? Convolution r...Read more
How to Boost Your Engagement with Visual Content
People are visual creatures. Nearly half of the human brain is dedicated to processing what we see, and we process images in as little as 13 milliseconds (to put it in context. a blink of the eye take...Read more
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Cox the Saddler
23 High St, Chesham, Buckinghamshire, HP5 1BG, England.
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Home | Saddlery | Horse Grooming Accessories | Horse Combs
Horse Mane comb
|NEWS: We have opened a Leathergoods Repair Service, so if you want us to help with any repair, just email firstname.lastname@example.com, ideally attaching a picture. We offer a free, no obligation quotation.
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Product Code: J115Small plastic horse mane comb. Colour may differ from that shown.
Price: Ł0.60 (Ł0.50 ex VAT)
(Approx. 0.68 USD US Dollars, 0.57 EUR Euros, 75.28 JPY Japan Yen)
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|Horse hair body brush for horses
Classy adult body brush for general horse grooming. Lovely lacquered wooden body and quality horse hair bristles.
Price: Ł10.21
(Ł8.51 ex VAT)
(Approx. 11.57 USD, 9.68 EUR, 1,281.31 JPY)
|Velvet Browband in 3 Colours
This leather browband for horses is made by E. Jeffries in Walsall England from best quality English bridle leather. The browband is covered in ribbons of 3 colours to form a traditional diamond pattern on the horse's brow. It is available in Shetland, pony, cob and full horse sizes. Make your choice of size and colour using the pull down menu. Please note yellow is no longer an option as we are unable to sources cotton velvet ribbon in yellow.
Price: Ł13.90
|Canter Coat Shine Conditioner
Canter Coat Shine Conditioner Conditions the coat whilst giving a long-lasting shine. Helps repel grass stains and soiling of the coat. Provides an oil-free shine; does not make the coat greasy.
Price: Ł8.06
(Ł6.72 ex VAT)
(Approx. 9.13 USD, 7.65 EUR, 1,011.80 JPY)
Would you like to check stock availability? Call Sara or Paul on 01494 775577 (if not from UK please call 0044 1494 775577) Mon-Sat 9.30 a.m. to 5.00p.m. Please Note: We do not accept orders by telephone and enquiries are better placed by email too. When ordering on-line, please note that we do not deduct any money from your credit card, until the goods are being despatched. Please also note that you may alter the quantity of goods in your basket at any time.
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Dear Customers, Don't struggle to identify the suitcase wheel or spare part you need. Email me a picture, email@example.com or call us on 01494 775577.
Customers SayReceived today, well packed and all entire. My thanks.
I only found your web site when looking for ferrules and took a chance on a spare umbrella. Very pleasing. Thanks again.
Geoff Mc., North Yorkshire
Yes got it! Thanks so much, I'll remember your company next time I need something.
Mark K., Berkshire
Thank you so much for the "martingale horse brass holders." They are exactly what I need to display the horse brass I accumulated when I lived in the UK, and of course I couldn't find them in the US. I looked here and could not find anything that would work. I did have one custom made but it was very pricy. Thank goodness for the internet and your "forward thinking" in advertising.
Marilyn M., Montana
Just a quick email to say thanks very much for despatching the order so quickly. I got it on Wednesday and was very pleased.
Emily McC, Belfast
Wow! The new wheels I ordered yesterday arrived by recorded delivery this morning already. Thank you.
Michael L., Hereford
Great service, thanks!
Kate D., Warminster
Safely received. Just the Job. Excellent service.
Thank you Paul
Keith C., Birmingham
Just to let you know that I received my walking stick today and I'm very pleased with it. :) Many thanks for such an efficient and speedy service, I will be recommending your company to others.
Carole A., London
Thank you for producing such a really nice gift , one always struggles with non-smoking, non-golfing people. It is so lovely that I am about to place orders for two more as Christmas presents for the men in my family.
M. Stoney, Darlington
I commend your very good service and the manner and attitude which went with it. Thank you.
Stuart S., Essex


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Wanna Join My Gang?
This entry was posted in General Notices, Village Hall Activities and tagged village events, village hall. Bookmark the permalink.
Next: The Millennium Pavilion on Thursday 19th October at 7.30 pm
Live Jazz in the Village Hall, bar opens at 7pm and the music at 7.30pm. Tickets on sale from Les Ellis
Incendies (15) on Sat 7th October 2017, doors open at 7pm


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Music Gear News
PLASA 2010: Dynacord launches the PowerMate 3 powered mixer
Dynacord has launched the PowerMate 3 at PLASA 2010, a third generation of the powered mixing standard offering greater power and functionality.
Despite considerable reductions in the weight, size and power consumption of the PowerMate, the latest version of this highly successful powered mixer offers an expandable bus structure and new features.
"We have integrated only the finest components into the design" said Dynacord's senior product managaer, Martin Traut.
"Without exception, we have chosen uncompromising solutions - from the extremely low-noise microphone preamplifiers in the mixing section to the six AUX sends and state-of-the-art Class D high performance power amplifiers".
"Every link of the signal chain complies with the highest concert sound requirements. And by integrating the four-in/four-out USB port, we have fulfilled an oft-expressed wish of our customers".
Since its launch in 1997, Dynacord's PowerMate has been a pro audio and MI industry standard selling more than 100,000 units making it the world's most popular powered mixer, not to add the numerous awards.
The latest incarnation is equipped with a professional mixing surface optimized for live performance, with a choice of 10, 16 or 22 channel strips.
Features include vocal voicing filters, six AUX busses, low-noise microphone preamplifiers, low cut filters and 11-band graphic equalizers.
The company promises 'concert level' sound performance and the new USB 2.0 audio interface simplifies tasks such as live recording, playback and interval music.
The mixer's Class D power amplifier outputs two channels of 1,000 Watts RMS (into four Ohms).
An innovative power management system makes it possible to adjust the output to suit different cabinets (including those with a somewhat lower power-handling capacity), while retaining the full dynamic range of the power amplifier.
The usual protective circuitry found in all Dynacord power amplifiers is also built in, ensuring operating safety and reliability.
The PowerMate 3 is also equipped with an extensive effects section, comprising two independent (and separately editable) effects processors, each with 100 factory presets optimized for live performance.
20 user presets, remote control (via MIDI), tap delay, USB configuration and a large, contrast-rich, OLED display are also provided.Posted on 14 Sep 2010 10:17 to category : Instruments News
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Recent Music News
The Roland SPD: ONE ELECTRO Trigger Pad is a next level sound solution developed to enhance and diversify your performances, giving your playing extra depth.
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Ads ruined this app.
Ads ruined this app.
Have had this for a few years now and it keeps getting better.
The applewatch complication doesnt update. When the app is opened, on the watch, it displays "No Games Today". Just so you know, its Sunday and Im watching it on tv TODAY! FIX THIS! Its a complication; not complicated.
Honestly the best app for sport scores, stats, and news
This apps fulfills my fundamental need for scores. It used to be my only sports apps but now competes with others on my iPhone. The ads slow this down. It would be nice to tag news articles and filter only the ones for your team when you drill down on your team and hit news. I like this app but its not best of breed anymore
Please add these Sports leagues; OHL, WHL, QMJHL, and the AHL! If you guy have College Basketball, College Football and you guys recently added College Hockey and College Baseball I dont see why you guys couldnt add the leagues I mentioned above. •Also video ads automatically play which uses your data.• Thanks
The frequency and interruptive nature of the ads used by this app ruin an otherwise great sports app. As I scroll through my tailored news (showing stories about teams Ive starred), in-line ads load after I scroll past them, forcing me to read through headlines Ive already seen. Its a constant accordion effect. Unfortunately, Ill have to delete this app and search for a replacement. Its a shame because the app is great aside from the in-line ads.
You screwed this app up!! Its slow. full of data eating ads. No injury info. No nothing. I deleted this one!
ios 10.0.2 hasnt been able to update for days. whats up?
Wish WWE was supported. until then back to yahoo. Also today widget is messed up on iOS 10. Not showing complete scores.
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Can you add European hockey to the next update?? Thanks.
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Fix the bugs when you say " bug fixes"
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This app has everything, from fantasy football to golf. Best app I ever bought


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The tumor suppressor protein p27Kip1 plays a pivotal role in the control of cell growth and metastasis formation.
Several studies pointed to different roles for p27Kip1 in the control of Ras induced transformation, although no explanation has been provided to elucidate these differences. We recently demonstrated that p27kip1 regulates H-Ras activity via its interaction with stathmin.
Here, using in vitro and in vivo models, we show that p27kip1 is an important regulator of Ras induced transformation. In H-RasV12 transformed cells, p27kip1 suppressed cell proliferation and tumor growth via two distinct mechanisms: 1) inhibition of CDK activity and 2) impairment of MT-destabilizing activity of stathmin. Conversely, in K-Ras4BV12 transformed cells, p27kip1 acted mainly in a CDK-dependent but stathmin-independent manner.
Using human cancer-derived cell lines and primary breast and sarcoma samples, we confirmed in human models what we observed in mice.
Overall, we highlight a pathway, conserved from mouse to human, important in the regulation of H-Ras oncogenic activity that could have therapeutic and diagnostic implication in patients that may benefit from anti-H-Ras therapies.
The tumor suppressor protein p27kip1 (hereafter p27) was originally identified as a cyclin/CDK inhibitor, in particular of the CDK2-containing complexes[1, 2]. Subsequent studies demonstrated that it is also implicated in the regulation of several other biological activities, such as differentiation, apoptosis, motility and autophagy [1, 2].
Formal demonstration that p27 is a fundamental negative regulator of cell cycle progression with tumor suppressor properties primarily arose from the characterization of p27 knock-out mice[3-5]. Interestingly, most of the phenotypes of p27 null mice are reverted by the concomitant knock-out of the microtubules (MT)-destabilizing stathmin [6], demonstrating that p27/stathmin interaction plays a role in the control not only of cell motility [7-11] but also of cell proliferation[6, 12]. Mechanistically, we recently showed that p27 controls H-Ras-driven proliferation acting on its intracellular recycling and mono- bi-ubiquitination [12].
A large body of literature exists focusing on the cooperation between Ras and p27 during tumor onset and progression. It is widely accepted that p27 expression impacts on Ras-driven tumor progression. Interesting differences have been noted when p27 knock-out mice have been challenged with tumorigenic models relying on H-Ras versus K-Ras mutations. In tumors driven by K-Ras mutations, such as the urethane-induced [13, 14] or K-RasG12D-induced [15] lung cancers, p27 acts as a haploinsufficient tumor suppressor gene and the loss of one allele is sufficient to induce the maximum oncogenic cooperation. Conversely, in H-Ras-driven tumorigenesis, such as the MMTV-H-RasV12-induced breast and salivary gland cancers [16] or the DMBA/TPA skin carcinogenesis model [17], p27 acts as a classical tumor suppressor gene and loss of one p27 allele does not result in enhanced tumor growth. In these settings, many of the H-RasV12/p27 null tumors displayed features characteristic of highly aggressive tumors [16]. Accordingly, it has been postulated that p27 controls cell H-RasV12-driven transformation via not only the inhibition of the cyclin/CDK/RB pathway but also via RB-independent pathways [18].
We have recently observed that p27 participates to the regulation of H-Ras activity by modulating its recycling and ubiquitination [12]. Since H-Ras and K-Ras are differently regulated by recycling and ubiquitination [19, 20], it is expectable that p27 could differentially act in H-Ras versus K-Ras-driven tumor progression. Here, we address this hypothesis using different in vitro and in vivo model systems.
We previously observed that p27 null cells and mice displayed higher Ras activity, due to different recycling and decreased mono-bi-ubiquitination [12]. To test if these differences may have any role in cell transformation, we generated and characterized H-RasV12-transformed WT and p27KO 3T3 fibroblasts [7, 21]. Endogenous p27 expression was maintained in H-RasV12 overexpressing-p27WT 3T3 cell clones (Figure (Figure1A).1A). WT and p27KO H-Ras cells were both less sensitive to serum deprivation than not transformed cells (Figure (Figure1B1B and Supplementary Figure S1A). Yet, p27KO H-RasV12 cells displayed increased S phase population when compared to WT H-RasV12 cells, in exponential growth (Figure (Figure1B)1B) and after contact inhibition (Figure (Figure1C).1C). Furthermore, they entered into the cell cycle sooner than WT H-RasV12 cells (Supplementary Figure S1A and B). Accordingly, in exponentially growing conditions p27KO H-RasV12 cells had a significantly lower doubling time (Supplementary Figure S1C), and proliferated significantly more than the WT counterpart (Figure (Figure1D1D and and1E1E).
In agreement with our previous observation [7, 8, 21, 22], WT versus p27KO-transformed cells displayed different ability to move, both in three-dimensional (3D)- (Supplementary Figure S1D) and in two-dimensional (2D)-extracellular matrix contexts (Supplementary Figure S1E), but not when 2D-migration was performed on plastic dish (Supplementary Figure S1F).
Subcutaneous injection of 1×106 WT H-RasV12 cells in nude mice (n = 5) were not sufficient to give rise to tumors, while the same number of p27KO H-RasV12 cells determined the appearance of tumor masses within 10-12 days (data not shown). Using 2×106 cells, all mice injected with WT H-RasV12 cells developed slow growing tumors within 15-17 days, but p27KO H-RasV12 cells formed fast growing tumors within 5-10 days (Figure (Figure1F)1F) as evidenced by differences in the explanted tumor masses (p < 0.0001, n = 5/clone), with no appreciable difference among the clones utilized (Figure (Figure1G1G).
In line with our previous results showing that p27/stathmin interaction modulates H-Ras activity [12] we showed that p27WT but not the p271-170 mutant (unable to bind stathmin) [7-9, 12, 21] reduced ERK1/2 phosphorylation when reintroduced in p27KO cells (Supplementary Figure 2A). In vitro experiments using growth curves (Figure (Figure2A)2A) and soft agar assays (Figure (Figure2B)2B) and in vivo experiments (Figure (Figure2C)2C) using subcutaneous injections, showed that p27KO H-RasV12 fibroblasts re-expressing p27WT or p271-170 were barely (in vitro) or not (in vivo) affected in their growth by either p27WT or p271-170. However, p27WT, but not p271-170, reduced the ability of p27KO H-RasV12 transformed fibroblasts to move in 3D-Matrigel (Figure (Figure2D)2D) and to intravasate, extravasate and settle at distant sites in nude mice, as demonstrated by the presence of cells expressing the H-RasV12 transgene in the blood and in the lungs of mice bearing subcutaneous tumors (5 mice/cell clone) (Figure (Figure2E2E and and2F2F).
To test whether stathmin was involved, at least in part, in determining the different in vitro and in vivo growth observed in WT versus p27KO H-RasV12 transformed fibroblasts, we used 3T3 fibroblasts derived from WT, p27KO and also double knock-out (DKO) for both, p27 and stathmin C57BL/6 embryos [12, 22]. Growth curve experiments confirmed that transformed p27KO cells grew much faster than WT cells and showed that DKO H-RasV12 cells displayed an intermediate growth rate (Figure (Figure3A)3A) and western blot analyses demonstrated higher ERK phosphorylation in p27KO H-RasV12 than in WT and DKO fibroblasts, both in basal conditions (Supplemental Figure S2A) and following serum (FBS) or Epidermal Growth Factor (EGF) stimulation (Figure (Figure3B3B).
Upstream activators of ERK include the H-Ras-related small GTPase RhoA that is also regulated by p27 [22, 23]. RhoA signals to ROCK1 to control both cell proliferation and migration [24, 25]. To distinguish the effects of p27 on H-Ras and RhoA we pharmacologically inhibited H-Ras (FTI-276) or ROCK1 (Y27632). FTI-276 treatment abrogated the differences in ERK phosphorylation between the different genotypes, while Y27632 treatment did not (Figure (Figure3C).3C). When used in growth curve experiments, both inhibitors reduced the proliferation of H-RasV12 transformed cells of all genotypes (Figure (Figure3D3D and and3E).3E). However, only FTI-276 inhibitor abrogated the differences in cell proliferation between p27KO and DKO cells (Figure (Figure3D).3D). Similar results were observed in soft agar assay experiments (Supplementary Figure S2B).
These results, along with the data collected on normal fibroblasts [12], suggested that p27/stathmin interaction through the regulation of H-Ras/ERK activity partially controls H-RasV12 driven transformation. If this was really the case, then no difference in cell transformation should be observed between p27KO and DKO fibroblasts transformed with K-Ras4BV12 oncogene. H-Ras and K-Ras4B (alternative splicing of exone 4 of K-Ras gene) are highly homolog proteins (83% aminoacid identity in the first 165 aminoacids) with an hyper variable C-terminus (24 aa) which comprises the membrane targeting sequence (Figure (Figure4A)4A) [26]. The different hyper variable region results in several unique features, such as their sub-cellular localization and their functional regulation. H-Ras and N-Ras, but not K-Ras4B, require recycling to be fully activated [19, 20] and H-Ras and N-Ras, but not K-Ras4B, are inhibited by mono-bi-ubiquitination [20]. In line with these notions, we observed that K-Ras4BV12 was not mono-bi-ubiquitinated either in the presence or absence of p27 and stathmin (Figure (Figure4B4B).
K-Ras4BV12 p27KO and DKO transformed cells displayed similar levels of ERK1/2 phosphorylation (Figure (Figure5A)5A) and proliferated fairly at the same extent but significantly faster than WT cells (Figure (Figure5B).5B). No substantial difference in the transformation efficiency was detectable by soft agar assay between p27KO and DKO K-Ras4BV12 cells (Figure (Figure5C).5C). We next analyzed, by pull down assay, the levels of GTP-bound active Ras proteins in WT, p27KO and DKO cells transformed with H-RasV12 or with K-Ras4BV12, in basal conditions and after stimulation with serum. As expected all transformed cells displayed constitutively active Ras, whose activity only slightly increased upon serum stimulation (Figure (Figure5D).5D). However, basal and serum-stimulated H-RasV12 (but not K-Ras4BV12) activities were significantly higher in p27KO when compared to WT and DKO transformed cells (Figure (Figure5D),5D), supporting the possibility that regulation of H-Ras ubiquitination by p27 and stathmin participated in the control of its activity.
In all experiments reported above, we consistently observed that both H-RasV12 and K-RasV12 transformed immortalized 3T3 WT cells with very low ability, especially when used below passage 45 (Figures (Figures1,1, ,2,2, ,3,3, ,4,4, ,5).5). This observation pointed that the p27/CDK/RB pathway plays a fundamental role as gatekeeper from Ras-induced cell transformation. Our results also showed that this function cannot be fully rescued by the reintroduction of human p27WT in p27KO transformed cells. To exclude species-specific effects due to the use of human p27 (h-p27WT) in rescue-experiments, we re-performed some of the same assays in p27 null H-RasV12 transformed cells, reintroducing the mouse p27, either wild type (m-p27WT) or mutated in the binding to Cyclin/CDKs complexes [8, 12, 27] (m-p27CK-) (Supplementary Figure S3A and B). In line with the results obtained with the human protein, m-p27WT and mp27CK- proteins only slightly affected the ability of H-RasV12 transformed cells to grow in culture or in soft agar (Supplementary Figure S3C and D) but significantly reduced cell motility (Supplementary Figure S3E).
To avoid the possible bias due to clonal selection and in the generation of 3T3 cells we next concomitantly transduced with and SV40 Large TAg (LgTAg) and with H-RasV12 (Figure (Figure6A)6A) or K-Ras4BV12 (Figure (Figure6B)6B) primary MEF of the different genotypes. Since SV40 LgTAg oncogene simultaneously inactivates p53 and RB, we anticipated that in this model the relevance of CDK-inhibition by p27 would be less pronounced.
In this model p27KO transformed with LgTAg and H-RasV12 grew at higher extent than the correspondent WT and DKO cells both in culture and in anchorage independent manner (Figure 6C, 6E and and6G).6G). Correspondent cell clones transformed with K-Ras4BV12 proliferated all at similar level, in the presence or absence of p27 (Figure 6D, 6F and and6H6H).
Similarly, tumors from LgTAg/H-RasV12 transformed p27KO MEF had significantly higher volume (Figure (Figure7A)7A) and number of Ki67 positive cells (Figure (Figure7B7B and and7C),7C), when compared to both WT and DKO counterparts. Conversely, LgTAg/K-Ras4BV12-transformed MEFs of all three genotypes displayed similar tumor volume (Figure (Figure7A)7A) and Ki67 expression (Figure (Figure7B7B and and7D).7D). In explanted tumors, the expression of the Ras-ERK downstream targets Egr-1, c-Fos and Jun-B was higher in p27KO cells only in the presence of H-RasV12 (Figure (Figure7E7E).
To evaluate the relevance of our findings in human cancer, we chose the soft tissue sarcomas (STS) as human counterpart of transformed fibroblasts. Following proliferative stimuli, MES-SA cells (established from primary STS) displayed high levels of p27 coupled with low levels of ERK1/2 activation and EGR1 expression, while HS-913T cells (established from metastatic STS) displayed low/null p27 levels, high ERK1/2 activation and EGR1 expression (Figure (Figure8A8A and and8B).8B). Using HT1080 cells, (a STS cell line harboring the N-RasQ61K mutation and a well-known model for Ras-driven transformation [28]) we next shown that Ras activity was reduced by one third by the overexpression of p27WT or p27CK- (Figure (Figure8C).8C). Since N-Ras shares the same recycling- and ubiquitin-mediated regulation of H-Ras [19, 20], our result supported the hypothesis p27 could control H/N-Ras activity in human STS. Accordingly, in a panel of already characterized human sarcoma specimens [7], low cytoplasmic p27/stathmin ratio was significantly associated with high levels of ERK1/2 phosphorylation (Figure (Figure8D)8D) and, when sarcoma specimens were segregated in primary vs metastatic tumors, we observed that metastatic tumors specimens displayed a significant lower p27/stathmin ratio coupled with a significantly higher ERK activation (Figure (Figure8E8E).
Finally, using BRAF-mutated colorectal carcinoma cells (i.e. Colo-201 and Colo-205 in Supplementary Figure S4A and B) and HER2-overexpressing mammary carcinoma cells (i.e. SK-BR-3 and MDA-MB-453 in Supplementary Figure S4C), we consistently detected an inverse correlation between p27/stathmin cytoplasmic ratio and the activation of ERK1/2 and the expression of EGR-1 (Supplementary Figure S4A-C). Importantly, knock-down of p27 in SK-BR-3 cells increased ERK1/2 phosphorylation (Figure S4D) and low cytoplasmic p27/stathmin ratio was significantly associated with a higher level of ERK1/2 phosphorylation in primary breast carcinomas (Supplementary Table S1 and Supplementary Figure S4E).
The results presented in this manuscript highlight several interesting features of the tumor suppressor gene p27 in the control of cell Ras-induced cell transformation and metastasis formation.
The most salient observation regards the susceptibility of WT or p27-null immortalized fibroblasts to the transforming activity of H-RasV12 and K-RasV12. The fact that p27-null fibroblasts are more prone to transformation and that reintroduction of p27 expression is not able to fully revert their phenotype reinforces the concept that control of CDK activity by p27 represents a significant barrier against cell transformation. The combined use of Large T-antigen and H-/K-RasV12 (Figures (Figures55 and and6)6) further confirms the relevance of p27 in controlling CDK activity during cell transformation. Yet, once cells are transformed by Ras, re-expression of p27 limited cell motility but failed to properly control in vitro proliferation and in vivo growth. Since we were concerned by the possibility that accumulation of concomitant mutations and/or genetic alterations could somehow affect the phenotype of p27KO H-RasV12 cells, we repeated our experiments using 2 additional models (i.e. C57Bl6 3T3 and primary MEFs transformation), overall confirming the observation made using the Sv129 3T3 cells (Figures (Figures11 and and2).2). It is interesting to note that in the case of v-Src transformed cells, reintroduction of the degradation resistant p27T187A mutant, completely reverted the phenotypes of p27-null cells [21], suggesting that the pathways activated by RasV12 and v-Src differently impact on the tumor suppressor roles of p27. Yet, one limitation of this study resides in the difficulty to discern whether one, or more of the multiple functions of p27, in the control of CDKs activities, MT-stability, actin reorganization, gene transcription and mitotic division [1, 2] could render p27 null cells more prone to transformation independently from the oncogene used.
A second interesting finding is the different potential displayed by p27 in restraining H-RasV12 versus K-RasV12-4B induced transformation. Only in H-RasV12 transformed cells p27 controls not only CDKs activation but also Ras activity/localization via stathmin. Consequently, p27-null cells had a further advantage in cell growth and invasion that is likely dependent by the lack of feedback control of Ras exerted by p27 when located into the cytoplasm. It is interesting to note that a role for cytoplasmic p27 in the inhibition of Ras activity, via GRB2 binding, was previously reported [29]. Yet, it was not specified whether H- or K-Ras activity was tested. p27 expression was also reported to be necessary to mediate the inhibition of H-Ras-induced transformation induced either by STAT1 or by dominant negative Rho, likely in a RB-independent or partially dependent manner [18, 30]. More recent evidences demonstrated that in MEFs and in urinary bladder HT1197 cells, carrying a N-Ras Q61R mutation, p27 inhibits cell motility likely by reducing ERK activation [31], independently supporting our findings. Overall these data point to p27 as a relevant regulator of Ras activity in a cytoplasmic and CDK-independent manner.
Our work presents the limitation due to the use of constituvely active Ras mutant vectors. It has to be considered that the K-Ras4B gene used in this work is an alternatively spliced version of the K-Ras gene that could represent only a minor portion of the total K-Ras transcribed, as we observed in a model of skin carcinogenesis [40]. Thus, our model could not fully recapitulate the activation of endogenous K-Ras that is transcribed as K-Ras4A and K-Ras4B, with the former still subjected to recycling to be fully activated [19, 20].
The role(s) played by p27 when located in the cytoplasm are at the center of an interesting scientific debate. It has been considered either a cellular escamotage to inactivate nuclear p27 [1, 32] or a more complex way to control other signaling pathways and processes [12, 23, 29], such as cell death and autophagy [33, 34] or cell motility [7, 23]. Most of these activities have been attributed to the C-terminal portion of p27, containing several important regulatory elements. It is interesting to note that, at least in MEN syndrome [35], breast cancer [36, 37], intestine neuroendocrine tumors [38] and prostate cancer [39], the CDKN1B gene (encoding for p27) is frequently mutated in the C-terminal portion of the protein [36-39]. One of the mutations, repeatedly identified in human breast cancers, is the E171®Stop [36, 37] that results in a truncated protein (p271-170) that we have characterized here and in previous publications [7-9, 12, 21].
Our data show that p27 and stathmin regulate in concert H-Ras but not K-Ras activity. This evidence could explain the contradictory tumor suppressor roles ascribed to p27 in different mouse models of cancer. In the presence of K-Ras mutations, p27 acts invariably as a haploinsufficient tumor suppressor gene [13-15]. In the presence of H-Ras, the complete loss of p27 is necessary to favor tumor growth in mice [16, 17], suggesting that the presence of one p27 allele is still able to restrain cell proliferation. According to this model, the fact that Ras-MAPK pathway activation results in phosphorylation of S10 and cytoplasmic delocalization of p27, likely represents a feedback control loop of particular importance to prevent unwanted cell cycle entry when the mitogenic extracellular stimuli are below the required threshold.
High levels of stathmin expression have often been linked to the acquisition of a metastatic phenotype [41]. The use of knock-out mice and cells allowed us to exclude a primary role of stathmin in the onset of several type of primary tumors in mice [40], suggesting that the function here described for p27/stathmin interaction can be unmasked in normal mice, cells and tissues only in the context of p27 absence. This concept may be of particular relevance in human tumors where p27 levels and localization are finely regulated and may also explain the contradictory results reported so far for stathmin in the control of cell growth, when gain-of-function [42, 43] or knock-out [44, 45] models were considered.
Successful targeting of the Ras-MAPK pathway represents a promising tool to treat aggressive human cancers, but optimal selection is needed to identify patients who would benefit from such therapies. Our findings suggest that evaluation of p27 and stathmin expression may contribute to this selection. This is particularly relevant for breast cancer, where Ras oncogenes are infrequently mutated but often hyperactive [46] and p27 mutations could be driver oncogenic events [36, 37].
Detailed description of the material and methods used is provided in supplementary material.
Sarcoma and breast cancer tissues were collected at CRO Aviano, Italy and stored in the Institutional Biobank, provided that the specific informed consent was obtained from the patient. Scientific use of biological materials was approved by the Internal Review Board (IRB) of CRO Aviano (# IRB-07/2015).
All animal experimentation were reviewed and approved by the CRO institutional Animal Care and Use Committee (OPBA), authorized by Italian Ministry of Health (# 616/2015-PR) and conducted according to that OPBA's guidelines.
Primary tumors were established by subcutaneous injection of 1×106 or 2×106 transformed cells into the flanks of female athymic nude mice (Harlan, 7-8 weeks of age). Tumor growth was monitored every other day for up to 26 days from injection.
Primary wild type (WT), p27 knock-out (p27KO) and p27/stathmin double KO (DKO) mouse embryo fibroblasts (MEF) were prepared from embryos at day 13.5, according to standard procedures [6, 7]. 3T3 fibroblasts were generated from primary MEFs, as described [47]. MEF, 3T3 fibroblasts, 293T/17, HEK 293, MDA-MB-453 and SK-BR-3 human mammary adenocarcinoma cells and MES-SA, HS-913T, HT-1080 sarcoma cell lines, were all cultured in DMEM supplemented with 10% FBS (Sigma). Colo-201 and Colo-205 human colorectal adenocarcinoma cells were cultured in RPMI-1640 supplemented with 10% FBS (Sigma).
3T3 fibroblasts were transformed using H-RasV12 (gently provided by Dr. R. Baserga) and K-Ras4BV12 (from ADDGENE, donated by Dr. T. Jacks) cDNAs both cloned in pMSCV-hygro retroviral vector (Clontech). Primary MEFs were transformed using concomitantly SV40 Large TAg (provided by Dr. R. Maestro) and pMSCV-Hygro-H-RasV12 or pMSCV-Hygro-K-Ras4BV12. Human p27WT or p271-170 were described [7, 21, 22]; mouse p27WT and p27CK- cDNAs were provided by Dr. Bruno Amati.
Proliferation assays include: growth curve experiments, using the Trypan Blue exclusion test and the MTS assay (Promega); cell cycle distribution using flow cytometry; BrdU incorporation assay (Roche); soft agar assays; and tissues staining with Ki67.
Motility assays include were performed essentially as described [7, 21, 22] and include 3D-Matrigel™ evasion assay ; transwell-based migration assay using HTS Fluoroblok™ coated with 20μg/ml fibronectin (Sigma) and wound-healing assay.
A total of 17 sarcoma (leiomyosarcomas and fibromyosarcomas) and 37 breast tumor specimens were collected and diagnosed at Centro di Riferimento Oncologico (CRO) of Aviano (Italy), according to the World Health Organization (WHO) criteria. Sarcoma samples were described elsewhere [7] and derived from primary (n = 8) or metastatic samples (n = 9). Breast cancer specimens derived from locally advanced primary tumors, as better specified in Supplementary Table 1.
We thank Dr. R. Maestro, Dr. T. Jacks, Dr. B. Amati and Dr. R. Baserga for providing reagents and all members of the S.C.I.C.C. lab for helpful scientific discussion.
The authors have declared that no conflict of interest exists.
This work was supported by Associazione Italiana Ricerca sul Cancro (AIRC) to GB (IG 12854) and by CRO Intramural research grant to GB. IS is a recipient of AIRC/FIRC Fellowship.
Author ContributionsB.B. and G.B. designed the research; I.P., L.F., S.B., I.S., F.C., M.C., S.D.A. and S.B. performed the experiments, developed the methodologies and analyzed the data; T.P. and V.C. provided pathological analyses of human and mouse tumor specimens; S.M. and M.S., provided reagents/tools; and B.B. and G.B. wrote the paper.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text: Film review: Nuns on the Run By Chris Hicks Published: March 30, 1990 12:00 a.m. Updated: April 3, 1990 12:00 a.m. + Leave a comment seret News Chris Hicks firstname.lastname@example.com Add a comment Like that? Read this. Sept. 22, 2017 Salinger biopic 'Rebel in the Rye' seeks to know man through relationships Sponsored by New York Code + Design Academy 5 powerful ways to take your career to the next level (and beyond)


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OCR Gateway GCSE Combined Science Revision Guide
Written by Collins GCSE
Illustrated by
Part of the Collins GCSE 9-1 Revision Series
OCR Gateway GCSE Combined Science Revision Guide by Collins GCSEExam board: OCR Gateway Level & Subject: GCSE Combined Science First teaching: September 2016 First exam: June 2018 This Science Revision and Practice range contains clear and accessible explanations of all the GCSE content, with lots of practice opportunities for each topic throughout the book. Based on new research that proves repeated practice is more effective than repeated study, this book is guaranteed to help you achieve the best results. There are clear and concise revision notes for every topic covered in the curriculum, plus five practice opportunities to ensure the best results. Includes: * quick tests to check understanding * end-of-topic practice questions * topic review questions later in the book * mixed practice questions at the end of the book * free Q&A flashcards to download online * an ebook version of the revision guide
About the Author
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Book Info
288 pages
Interest Age: From 14
Collins GCSE
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Collins an imprint of HarperCollins Publishers
Publication date
31st August 2016
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Godzilla Movies
Log in to copy items to your own lists.
Godzilla (1954)
American nuclear weapons testing results in the creation of a seemingly unstoppable, dinosaur-like beast. (96 mins.)
Godzilla, Mothra and King Ghidorah: Giant Monsters All-Out Attack (2001)
Three ancient guardian beasts awaken to protect Japan against Godzilla. (105 mins.)
Director: Shûsuke Kaneko
Godzilla, King of the Monsters! (1956)
A 400-foot (122-meter) dinosaur-like beast, awoken from undersea hibernation off the Japanese coast by atomic-bomb testing, attacks Tokyo. (80 mins.)
Director: Ishiro Honda, Terry Morse
Godzilla vs. Megaguirus (2000)
Japan creates an artificial black hole device to trap Godzilla forever, but a test of the device creates new foes for Godzilla, car-sized dragonflies called meganula and their queen, Megaguirus. (105 mins.)
Director: Masaaki Tezuka
Stars: Misato Tanaka, Shôsuke Tanihara, Masatô Ibu, Yuriko Hoshi
Godzilla: Final Wars (2004)
Godzilla's fiftieth Anniversary project, in which Godzilla travels around the world to fight his old foes plus a new, mysterious monster named Monster X. (125 mins.)
Director: Ryûhei Kitamura
Stars: Masahiro Matsuoka, Rei Kikukawa, Don Frye, Maki Mizuno
Godzilla: Tokyo S.O.S. (2003)
One year after the fight between Godzilla and Mechagodzilla, the two monsters along with Mothra face off in a conclusive battle royale. (91 mins.)
Director: Masaaki Tezuka
Stars: Noboru Kaneko, Miho Yoshioka, Mickey Koga, Hiroshi Koizumi
Ghidorah, the Three-Headed Monster (1964)
After a meteorite unleashes a three-headed beast upon Tokyo, Mothra tries to unite with Godzilla and Rodan to battle the extraterrestrial threat. (93 mins.)
Destroy All Monsters (1968)
Female aliens take control of Earth's monsters and begin using them to destroy the human race. (89 mins.)
Stars: Akira Kubo, Jun Tazaki, Yukiko Kobayashi, Yoshio Tsuchiya
Godzilla vs. Destoroyah (1995)
The aftermath of the Oxygen Destroyer brings forth Destoroyah, a beast intent on killing Godzilla, who is on the verge of a nuclear meltdown. (103 mins.)
Godzilla vs. Biollante (1989)
After rising from his volcanic grave, Godzilla is threatened by a mutated rosebush. (104 mins.)
Director: Kazuki Ohmori
Godzilla vs. Mechagodzilla II (1993)
The United Nations assembles the ultimate weapon to defeat Godzilla, while scientists discover a fresh pteranodon egg on a remote Japanese island. (108 mins.)
Mothra vs. Godzilla (1964)
A greedy developer unwittingly hatches a gigantic baby moth upon Tokyo, while Godzilla strikes once again. (89 mins.)
Stars: Akira Takarada, Yuriko Hoshi, Hiroshi Koizumi, Yû Fujiki
Godzilla vs. King Ghidorah (1991)
Time travelers use Godzilla in their scheme to destroy Japan to prevent the country's future economic reign. (103 mins.)
Director: Kazuki Ohmori
Godzilla vs. Mechagodzilla (1974)
Apelike aliens build a robotic Godzilla to destroy Japan, and the true Godzilla may not be powerful enough to destroy it. (84 mins.)
Godzilla 2000 (1999)
Godzilla saves Tokyo from a flying saucer that transforms into the beast Orga. (99 mins.)
Stars: Hiroshi Abe, Naomi Nishida, Takehiro Murata, Mayu Suzuki
Godzilla and Mothra: The Battle for Earth (1992)
In the midst of another rampage by Godzilla, Mothra emerges to save the human race from Battra. (102 mins.)
Terror of Mechagodzilla (1975)
Godzilla comes to the rescue when an alien race rebuilds Mechagodzilla to destroy Earth's cities. A traitorous scientist gives them a second weapon: Titanosaurus. (83 mins.)
Godzilla 1985 (1984)
Thirty years after the original monster's rampage, a new Godzilla emerges and attacks Japan. (103 mins.)
Director: Koji Hashimoto, R.J. Kizer
Stars: Raymond Burr, Ken Tanaka, Yasuko Sawaguchi, Yôsuke Natsuki
Godzilla vs. Hedorah (1971)
From Earth's pollution a new monster is spawned. Hedorah, the smog monster, destroys Japan and fights Godzilla while spewing his poisonous gas to further the damage. (85 mins.)
Director: Yoshimitsu Banno
Godzilla vs. Gigan (1972)
A man begins to suspect that something is wrong about his employers; meanwhile, Godzilla and Anguirus are alerted to something strange going on. (89 mins.)
Godzilla (1998)
A giant, reptilian monster surfaces, leaving destruction in its wake. To stop the monster (and its babies), an earthworm scientist, his reporter ex-girlfriend, and other unlikely heroes team up to save their city. (139 mins.)
Director: Roland Emmerich
Stars: Matthew Broderick, Jean Reno, Maria Pitillo, Hank Azaria
Godzilla vs. Megalon (1973)
The undersea nation of Seatopia sends the gigantic Megalon to destroy the world above - and it's up to Godzilla and a size-shifting robot, Jet Jaguar, to defeat him. (82 mins.)


text:
West Indies
Shai Hope
Kraigg Brathwaite
Kyle Hope
Chris Gayle
Evin Lewis
Jermaine Blackwood
Kemar Roach
Jason Holder
Roston Chase
Kieran Powell
Test caps
One-Day International caps
Twenty20 International caps
Full name
Gary Daniel
October 29, 1986
Current age 30 years 328 days
Major teams Dominica
Feb 8, 2008
Portrait of Gary Daniel
© Stanford 20/20
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Rensburg Estates residential lettings team currently provide a first class service to both landlords and tenants with our unique full management service.
We offer prospective tenants quality accommodation throughout the Yorkshire region. We believe it is important that all our tenants are satisfied with their property and therefore, all properties available for rent will be clean, tidy and ready for occupation.
We are keen to ensure all maintenance issues are addressed quickly and efficiently for your enjoyment of the property throughout the tenancy. We have a team of approved contractors to meet general maintenance issues and emergencies.
Tenancy Application/References
When a suitable property to rent has been found ALL persons aged eighteen or over will complete an Application Form and will be named in the Tenancy Agreement. We will take up references on behalf of our client, the Landlord, which may include a bank reference for which your bank will charge a nominal fee, a credit reference, an employer's / accountant's and previous landlord reference (if applicable). A payment to indicate your commitment is required when you submit your application and is held against expenses. The payment is non-refundable should your application be unsuccessful or withdrawn. Please note that this money in advance does not constitute a tenancy and is the administration fee that is payable to Rensburg Estates.
Security Deposit
Before your tenancy commences you will be required to pay a security deposit. This deposit is held by Rensburg Estates as stakeholders or passed to the landlord.
The deposit is held against any damages or expenses arising during your tenancy and will not be released until after you vacate the property, all rent has been paid up to date and we have written confirmation of any costs for damages from both parties. No interest is payable to either party.
Tenancy Deposit Protection
From 6 April 2007 when you pay a deposit for an Assured Shorthold Tenancy, your landlord or agent must protect it using a government authorised tenancy deposit scheme. This is to ensure you get all or part of your deposit back when you are entitled to it and any disputes will be easier to resolve. Within 14 days of paying your deposit your landlord or agent is required to give you details about how your deposit is protected. This includes the contact details of the tenancy deposit scheme and what to do if there is a dispute at the end of the tenancy.
Inventory and Schedule of Condition
An Inventory and Schedule of Condition of the property, its contents, furniture, fittings and effects will normally have been prepared. It is important that you take care in agreeing the Inventory at the beginning of your tenancy as it will form the basis of any claim for damages, by your landlord.
Rental Payments
All rent is payable in advance by standing order. Should you experience any financial problems during the course of the tenancy it is essential that you contact Rensburg Estates or your Landlord immediately.
Tenant's Obligations
You should be aware that responsibility for the property rests with the tenant during any tenancy. It is particularly important that any gas or electrical problems or water leaks are reported immediately and the property is fully secured when you leave it unattended at any time.
During the winter months, necessary steps must be taken to prevent the freezing of the water, pipework andheating systems.
In leasehold properties, mainly flats and maisonettes, the tenant will be bound by the rules and regulations affecting all residents within the block contained in the head lease.
The Agent's Obligations
The obligations of Rensburg Estates will vary depending on which of the following services we provide for the Landlord:
- Letting and Property Management
- Letting Only
- Letting and Rent Collection
You will be advised which of the above apply prior to your tenancy commencing, should you need further details regarding a specific property please contact Rensburg Estates.
Make a payment
To make a payment to Rensburg Estates please contact 0871 227 2226 offices between 9:00 and 17:30 Monday to Friday.
Please have your credit or debit card handy to make a payment over the telephone. Please note a 1% charge will be made for debit cards and a 3% charge will be made for credit cards.


text:
I'm sure there are people interested in the Macintosh port of TableMaster aside from those in the backers' forums, so while I'm recovering from a head cold and a major overdose of turkey (and stuffing, and glazed carrots, and scalloped potatoes, and those tasty, tasty little onions) here's something of an update. (and a user-written table)
Category: News
I can't work. I can barely function. Sorry about that, but I am a human being, as much as I try to present my company-self as neutral in all things and separate from anything but writing software. I am a human being, and an American, and the events of this week have affected me badly. You really do not want what I might do to the code this week. I don't want to debug what I might do to the code this week, and it would waste all of next week, too, fixing it.
I'm not going to use this blog for a political rant — this is about TableMaster, not about what TableMaster's author thinks about politics, or about art or music or anything else for that matter. I can keep up the public impartiality that far. But right now, I can't write code — especially cross-platform code.
If I worked for someone else, I'd be using my personal days right now. As it is, I'm going to take this week off from Wintertree and quit trying to force myself to get any productive work done, because I've been trying and it's just not happening. Everything will be back on track next week, just a bit behind schedule.
And as a person, as Jean, not Wintertree Software, I will add this:
Everyone, be kind to one another. Even people you disagree with. Especially people you disagree with. We have seen what divisiveness and hatred can do; let us not embrace it ourselves and thereby prove it is right.
Online Store Open for Business!
The Wintertree Software website now has an online store for those gamers unable to get TableMaster at their local game stores. It's here: WINTERTREE ONLINE STORE. You can buy TableMaster, all the Table Packs, and even some Wintertree swag, like the TableMaster box art magnets and the flashlight pens.
Please note that at the moment, while digital-only versions are available everywhere, physical products are restricted to US shipping addresses (including APO and FPO) until some issues about overseas shipping get untangled.
If you're wondering just what tables come with any given Table Pack, each one has a "Notes" button next to its description that will pop up a window with a complete list.
Because I can't leave well enough alone….
All the Kickstarter copies of TableMaster, various Table Packs, etc., are out the door (and in the case of US shipments, in the backers' hands). There are no conventions in the near future. I'm (finally) almost over that wretched cold. I'm getting ready to do the Mac port. But … there was a bug. Not a big bug, but a bug that bugged me. So I went to fix it …
…and added two new commands to TableMaster, and added an option to two more.
Continue reading "Because I can't leave well enough alone…."
Packages Are Out the Door!
First, for the people who wondered why I've been silent since before GuildCon: I picked up a wicked head cold at GuildCon, and I've spent the past week-plus gasping for breath and clutching a box of tissues like it was life itself. I spent my birthday in bed, sniffling. 🙁 Blogging hasn't been much of a priority. Nor other complex things like standing up. I still feel like roadkill, but at least I can get up and move around now.
So, I just got back from the Post Office, mailing out TableMaster packages to the Kickstarter backers. Here's a picture of them all on the Post Office counter:
For Priority Mail, they say "if it fits, it ships." I hope they have a generous understanding of "fits" — some of those T-shirts did not want to go in their boxes. (when I tested originally, I used one of my shirts … and I wear Medium)
There are some pictures of what's in the package in the latest Kickstarter update, too.
So … product is shipped, head cold is on the retreat, and I can get back to coding. TM/Mac, here I come!
More New Commands
16 years ago, I thought TableMaster was mature. There were no new commands that needed to be added, no more features that anyone was going to need. Right. That was 16 years and 15 commands ago.
TableMaster is now officially in version 1.3 (y'all can get it on the beta site; next week I'll put it on the main site if nothing blows up in the interim) and it's got a couple of new ones. One is just a bit of convenience, but the other is fairly significant for how tables interact with other things.
Quick Pics
Just a quickie post here to say that 1) we haven't washed away yet, and 2) there are now pictures of the TableMaster product packaging on the Wintertree website.
Also, the endlessly delayed manual is now in the hands of the printer. Assuming the proof turns out okay, by this time next week the physical copies of TableMaster should be in the snail mail!
Manual Madness
I give up. I've tried. I've tinkered with font sizes until I'm seeing spots, I've ordered samples of fresnel magnifiers, I've specced super-thin paper, you name it. And it's not working. I just can't put ten pounds of manual into a five-pound bag.
20 years ago, TableMaster was smaller (it now has 13 new commands, more options for the existing ones, and more than 20 new output formatting codes). The manuals were wider and taller — they were a half-sheet, 5.5 x 8.5, instead of the 4.75 x 7.125 necessary to fit in a DVD box. And I didn't have any restrictions on the length; TableMaster was in an inch-thick box, so there was plenty of room for however many pages the manual needed. None of those is true anymore — the command set is bigger, the page size is smaller, and the box is much, much thinner. It. Just. Doesn't. Fit.
Everybody into the Boole!
There are three flavors of User Variables in TableMaster: ones with no variety specified (they were the originals), which are integers; Decimal variables, for floating-point numbers; and Text variables, for strings. There is actually a de-facto fourth kind, essentially Boolean variables.
George Boole was a 19th-century mathematician who formulated what came to be known as Boolean algebra, which dealt with variables having values of "true" and "false" rather than numeric values, usually represented as 1 and 0. If that sounds familiar — like the fundamental nature of digital electronics, from your watch to TableMaster — you're right: it is. Most computer languages have some sort of Boolean variable. Delphi (which is basically Pascal, named for another mathematician) certainly does. And so does TableMaster … sort of.
Cover art!
While I've been dealing with all the insanity of getting the manual to fit in the box (note: a package of nice little fresnel magnifiers arrived yesterday) I've also been working on the other problem: the fact that I can't draw. And there's only so much you can do with clip art. So I've been looking for an artist. Not just any artist — I needed someone whose style would be right for TableMaster, and who would "get" what it was all about. So, I've been checking out artists, and art. When I found Rick Hershey, of Fat Goblin Games, I knew I had the right artist.
If you're an old TableMaster customer, you'll remember this bit of artwork from inside the manual: