text:
Mayor-President Kip Holden announced at a news conference Monday that he has lined up $1 million to keep buses rolling in East Baton Rouge Parish, averting a potential shutdown of the Capital Area Transit System in October.
The immediate crisis was averted after the East Baton Rouge Parish Mortgage Finance Authority agreed to grant $500,000 to CATS and the state Office of Community Development stepped in with $500,000 in federal block grant funds for local communities affected by hurricanes Katrina and Rita.
Brian Marshall, CATS' CEO, praised Holden for his efforts to find CATS money to keep its doors open this year. However, Marshall said, after the news conference the transit agency faces a difficult budget year for 2012.
He said decisions will have to be made within the next two months about possible service cutbacks or fare increases to address a projected $2 million shortfall in 2012.
"The gap right now would be approximately $2 million," Marshall said.
A Blue Ribbon Commission Holden appointed to study long-term solutions for the city-parish's transit has recommended placing a combined sales and property tax proposal on the ballot in the fall of 2012.
Even if approved, Marshall said, the proceeds of the tax won't come in time to bail CATS out of financial difficulty next year.
Holden noted at the news conference that the Metro Council rejected a plan in January that CATS had put forward to solve its budget woes through a combination of service cutbacks and fare increases.
The mayor said he stepped in to try to find a short-term solution so CATS could keep its doors open even though the city-parish is not responsible for funding CATS.
CATS was created by state statute as a political subdivision of the state, but the law gives the Metro Council the power to appoint board members and approve rate adjustments and fare increases.
The funds obtained from the Mortgage Finance Authority and the Louisiana Office of Community Development will be used to leverage additional federal grants, wiping out the projected $1.29 million deficit CATS faced this year, Holden said.
"This means there will be no shutdown of service and no layoffs of employees," Holden said.
The mayor made the announcement from the Metro Council's chambers.
He stood at a podium in front of two large posters bearing signatures and the words: "Thank You Mayor-President Holden from 150 CATS Employees and Families and 10,000 Riders."
Holden said the public transit system is vital to residents who rely on buses for transportation to get to school, work, doctor visits and other destinations.
Holden said the $500,000 from the Mortgage Finance Authority comes as a grant and is from a pool of funds used to support ownership and economic development in East Baton Rouge Parish.
If CATS receives a dedicated funding source, the grant is to be converted to a loan and is to be repaid to the Mortgage Finance Authority over the next seven years, Holden said.
The mayor said he will support a tax proposal on the ballot next year that would provide CATS with a source of dedicated funds for its operations.
He said it would have been devastating for the community if CATS had been forced to shut down this year.
"Successful communities have successful public transit systems," Holden said. "We have to build one too."


text:
Question: In the sequential move game described in part b of
In the sequential- move game described in part b of the previous question, Levi Strauss engages in pre- play communication ( cheap talk). Levi Strauss tells Wrangler that it will match Wrangler's color choice if Wrangler chooses black and violet, but that if Wrangler opts for white, Levi Strauss will choose violet. Why would Levi Strauss want Wrangler to believe this claim? Should Wrangler believe it?
View Solution:
View Solution:
Answer to relevant QuestionsA thug wants the contents of a safe and is threatening the owner, the only person who knows the code, to open the safe. "I will kill you if you don't open the safe, and let you live if you do." Should the owner believe ...Walmart has a reputation for using a variety of legal means to prevent unionization of its stores by frustrating union organizers (Lichtenstein, 2008). Why would it make sense for Walmart to spend more trying to deter ...Show a game tree where the firm that moves second has a higher profit than one who moves first in the subgame-perfect Nash equilibrium.By next year, a stock you own has a 25% chance of being worth $ 400 and a 75% probability of being worth $ 200. What are the expected value and the variance? 1.5. Joanna is considering three possible jobs. The following table shows the possible incomes she might get in each job.For each job, calculate the expected value, the variance, and the standard deviation. If Joanna is averse ...
Post your question

subset: commoncrawl


text:
Katie Thomas - Broc
Added: Monday, January 19, 2009
Loading Katie Thomas - Broc Video
Katie Thomas - Broc
FORE! That's what you're supposed to say before you hit the white ball when you play pool. I learned all sorts of rules for playing pool. Like, when you hit the white ball in the hole, that's called a scratch and when you do that, you have to engage in sexual behavior with 2 black dudes. Well, that's what they told me at least. But when I heard that rule, I hit the white ball into a hole as fast as possible. Then I got to have some black pipes stuck in my holes. What a fun treat. I hope I didn't get the pool table messed up though. I can't remember if there were black seed stains on the felt when the brothers were done emptying their balls on my face.


text:
You ain't my type if ain't tryin' to be successful.
Turn-ons: Motivation, Initiative, Decisiveness, Wanderlust, Eye contact
Turn-offs: Time wasters, Phone addicts, Excuses
Cheat days
Sitting in weird positions
Laughing way too loud
Geeking out over superheroes
Finding the motivation to do things I don't want to do in order to get to where I want to be.
Books: Magic's Pawn, Hero, Harry Potter, Rich Dad Poor Dad
Movies: Avengers, Inception, Maurice, Bridesmaids
Shows: Avatar:TLA, One Piece, Charmed, The Amazing Race
Music: Yellowcard, 'N Sync, Donna Summer, Tyga
Food: Thai, Chinese, Pasta, Chocolate
Other people's stories
The places I'm going to visit
Financial freedom
Improving myself
My next adventure
Who to laugh with today


text:
Displaying 1 - 10 of 12 results
Sort by
Inquisitions: 1601
Abstracts of Inquisitiones Post Mortem For the City of London: Part 3
3 acres of land in Brickman Lane in Eastham, now or late in the tenure ... messuages and 2 other messuages late being 1 stable situate in a certain lane called Shoe lane in the...View result
1582 London Subsidy Roll: Farringdon Ward Within
Two Tudor Subsidy Rolls for the City of London, 1541 and 1582
Richard Smith (£5) [a] 5 Straungers Arnold Brickman (£20) 40 Martyn ... (£3) 3 Edward Lane esquier (£40) [a] 40 Mr [Blank] Hampton gen (£10) [a] ... Foster Lane. 'Harald ad arm' in margin. As...View result
London and Middlesex Fines: Henry VIII
A Calendar To the Feet of Fines For London and Middlesex: Volume 2, Henry VII - 12 Elizabeth
Hil. Anno 8. Edward Mountagu, George Lane, John Lane, and Thomas Arthure, ... Millis, Thomas Brickman, Richard Taillow, and William Quernby, and EdwardView result
Charles II - volume 165: July 27-31, 1666
Calendar of State Papers Domestic: Charles II, 1665-6
into bond of 1,000 l., with Wm. Brickman for his loyalty and good appearance. ... is reported; 10.000 men were in arms, headed by Sir George Lane's brother-in-law,View result
Bridge House Weekly Payments Book 1(2): Account for 1420-1
London Bridge: Selected Accounts and Rentals, 1381-1538
for quit-rents from the tenement/s in Honey Lane for Michaelmas term last, 16s. 8d. ... knight/s, for quit-rent of the Bridge's tenement/s in Honey laneView result
Index of places
Abstracts of Inquisitiones Post Mortem For the City of London: Part 3
Herts, 343. Brickleton, co. Berks, 38. Brickman Lane, East Ham, 295296. ... Mary-at-Hill, 135137. Abchurch Lane, 132, 254255. Acclome [Acomb ?], co. York, ... 6263, 303. Allhallows, Honey...View result
General Index: B
Calendar of State Papers Domestic: Charles II, 1665-6
Rob. letters of Bingley, Major Oliver, petition of Birchin Lane, London, ... of Bournmouth Boutwell, Mr. Bow Lane, London Street, London Bowden, Robt., ... Brickman, Wm. Bridewell, prisoners in...View result
General Index: B
Calendar of State Papers Domestic: Charles II, 1677-8
Lane, Thomas. Bagshot Park, house in, to be viewed for repairs Baillie, ... for oppressing one another Sampson. See Lane, Thomas. Birchington, in Thanet, ... Brickhill, the postmaster of Brickman...View result
London Bridge: Selected Accounts and Rentals, 1381-1538
All Hallows Gracechurch, 154, 303 All Hallows Honey Lane, 119, 316 All Hallows ... Billingsgate, 302 Billiter Lane/street [ Belyetterlane], 155, 303 Billyng, ... tie, iron, 79 Birch (Byrche), Nicholas, carpenter...View result
Index of persons and places
A Calendar To the Feet of Fines For London and Middlesex: Volume 2, Henry VII - 12 Elizabeth
Brickman, Thos, 27. Brigges, Anth., 39; Giles, 39. Bright, Hen., 43. ... Will., 85. Chamley, John, 115. Champion, Walter, 25. Chancery Lane, 86, 96. ... 4 (2). Charlyngton, 50. Charterhouse, 60, 77, 79. Cemetery...View result


text:
- Not to be confused with Carius Runellius.
Carnius Magius is an Imperial noble residing at Fort Frostmoth, and is the Factor of the East Empire Company's operations on Solstheim. Due to his title in charge of all Company business on the island of Solstheim.
As the main questline progresses, the Nerevarine will have to choose between Falco Galenus and Carnius.
- A Blocked Door
- Aiding and Abetting
- Bar Brawl
- Discovery in the Mine
- Drastic Measures
- Establish the Mine
- Hiring Guards
- Making a Choice
- Missing Supply Ship
- Race Against the Clock
- Razing the Forest
- Setting up Shop
- Stop the Messenger
- Supply Route Problems
- The Assassin
- To Catch a Thief
- Under Siege


text:
Community partners play a critical role to enable students, staff, alumni and Board members to give back to our communities and for our students to develop important interpersonal skills such as communications, teamwork and creative problem solving through volunteer and service learning opportunities.
Since 2014, staff and students contributed more than 24,250 hours of service to over 425 community projects over 4 service days. Metals students built instrument stands for a music outreach program, health students worked with seniors, business students provided marketing support to a community kitchen and more.
We would love to learn more about your organization and see how we can work together to transform lives and communities.
To get involved as a community partner, contact us at firstname.lastname@example.com.
Full-Time Programs
Online Courses
Part-Time Courses
How to Apply
Admission Requirements
Tuition & Fees
Scholarships, Bursaries and Awards
© Copyright 2017. All Rights Reserved.
Privacy Policy


text:
Dimitri Rassam
Co-Founder, ON Entertainment / Producer, Chapter 2 / Producer, Onyx Films
: ON Entertainment
Want to see projects Dimitri Rassam is developing? Visit Variety Insight .
Latest Headlines: Dimitri Rassam
A rock star's literature-inclined daughter faces loss and imminent motherhood.
Benicio del Toro is in final negotiations to play notorious Colombian cocaine trafficker Pablo Escobar in "Paradise Lost," a Panama-set €20 million ($25 million) thriller romance produced by Dimitri…
Millennium Entertainment has acquired U.S. rights to Juan Diego Solana's sci-fi romancer "Upside Down," starring Kirsten Dunst and Jim Sturgess.
An unborn's unlikely moniker is the simple but effective motor of "What's in a Name?" a French comedy adapted from a play by Matthieu Delaporte and Alexandre de la Patelliere.
Paris-based Onyx Films has boarded Alexandre Aja's "Cobra: The Space Pirate," a live-action film based on the cult Japanese manga, teaming with Studio 37, the film arm of European telco giant Orange.
"Kung Fu Panda" co-director Mark Osborne has been tapped to direct Gallic 3D toon "The Little Prince."
Top U.S. toon helmer Mark Osborne, who co-directed "Kung Fu Panda," has been tapped to direct Gallic S3D animated feature "The Little Prince."
The fact that Fidelite Films' Marc Missonnier and Olivier Delbosc snagged the adaptation rights for Europe's biggest movie franchise, Asterix -- beating former Asterix producer-director Thomas…
French producers Aton Soumache and Dimitri Rassam are preparing a stereoscopic 3D animated feature adaptation of author Antoine de Saint-Exupery's classic children's tale, "The Little Prince."
Army Archerd, whose 52-year run as a Daily Variety columnist made him unique among showbiz reporters, died Tuesday in Los Angeles of a rare form of mesothelioma, a lung cancer thought to be the…


text:
Jamboree 2015
Willowbank Raceway, Willowbank
29 August, 2015
The Jamboree is on 29 August, 2015 at the Willowbank Raceway, Champions Way, Willowbank.
The 25th Brisbane Jamboree is set to smash it out of the park with Australia's fastest Sport Compacts on tap & ready to roll, and four top Kiwi race teams crossing the Tasman to heat things up.
Scheduled for one huge day and night of racing, Jamboree is packing the best small engine/huge turbocharger combinations from Australia and New Zealand into one show that will see epic trans-Tasman match-ups and some world record smashing performances.
There is just as much happening at the Jamboree off the track with a massive Show-N-Shine, car audio sound offs, dyno comps, babe & bikini comps, automotive product displays, competitions, giveaways and much more. Spectator gates open from 7:30am.
For more info visit http://www.jamboree.com.au/news/item247.php
Repco Hoppers Crossing Fathers Day Show and Shine
Foresite Training Grounds, 1 Banfield Court, Truganina
29 August, 2015 (10:00 AM - 4:00 PM)
The Repco Hoppers Crossing Fathers Day Show and Shine is being held on the 29th August 2015 at the Foresite Training Grounds in Truganina.
All donations from the day will be donated to to a worthy charity / foundation. Entrants $15 per vehicle, gold coin donation for general public.
For more info visit https://www.facebook.com/RepcoHoppersCrossing
Fathers Day Car Show and Market
Ningi Community Hall, 1320 Bribe Island Road, Ningi
29 August, 2015 (10:00 AM - 4:00 PM)
The Fathers Day Car Show and Market is being held on the 29th August, 2015 at the Ningi Community Hall from 10am to 4pm.
Trophies awarded to different categories of cars and motorbikes. This is a fathers day themed event to enable us to assist Gaps in Mens Services.
For more info visit https://www.facebook.com/rainbowstarsfoundation?_rdr=p
Tumut Aces n Eights Show n Shine
Riverglade Caravan Park, Snowy Mountains Highway, Tumut
29 August, 2015 (2:00 PM - 4:00 PM)
The Tumut Aces n Eights Show n Shine is being held on Saturday August 29th at the Riverglade Caravan Park at 2pm.
Gold coin entry for entered cars only. Locals we hope to see you come down and check out the Rods and the stalls we will be having. Proceeds from your gold coin donation will be donated to a local organisation. See you all there.
For more info visit https://www.facebook.com/pages/Tumut-Rod-Custom-Aces-n-Eights-Inc/215252921821721


text:
Grey Wolf Hat by clearkidWatch
©2009-2017 clearkid
Commissioned by
Still tweaking the new base pattern that allows for these ears.
Idea (c) ~Mechashinobi-X
Photo and pattern (c) ~clearkid
Commissions: [link]
Tutorial: [link]
For the glory of God!
Still tweaking the new base pattern that allows for these ears.
Idea (c) ~Mechashinobi-X
Photo and pattern (c) ~clearkid
Commissions: [link]
Tutorial: [link]
For the glory of God!
Absolutely adorable! Love it!
i want it. D:
Do you have the pattern to this?!?!?!?!?!?!?!
Cute! ^.^
How much would you like for one of these? I cannot find it in commissions.
I dont know what to say. Is it cool? Is it cute? Or both?
this is so CUTE i made a profile just 2 c if i can buy 1 of your grey wolf hats
*le gasp!* I WOULD BUY THIS IF I FOUND SOMEONE TO MAKE ME ONE!!! this hat is super cute!!!!
u mind if i turn this into a knitting pattern?
do u have this item available to buy still
love, love, LOVE!! You should keep the ears as they are, cause they're PERFECT! ROWR
How did you add the design, Like the blue lines on the cheek, and the white pattern around the face/mouth. Was that seperate cloth sown on as well?
Yup, the whole face including the white part are all sewn on top of the grey hat.
also did you sew the eyes and other details on or are they like your eyes from the tutorial and colored on?
These are all sewn on, but you do whatever works best.
i love how you did the ears here. im just learning how to make hats like this. im just practicing with scrap fabric but i want to know. when you made the ears for this hat did you use wire? or did you just sew them on by hand in that position? ive got some pretty nice wire but if i sew them in the seam i cant make it look right lol. just curious if you have some tips lol
This is actually a modified pattern where I made two seams in the front and sewed the ears into those seams. I use to just sew the ears on by hand in a C shape so I wouldn't have to wire them.
I checked the tutorial, then looked back at this. This is most definitely made with a different pattern!
Would you be posting the pattern for this one anytime? I'd love to make a hat with this type of ears
Would you be posting the pattern for this one anytime? I'd love to make a hat with this type of ears
Cool! By any chance, are you selling these? And if so, how much would it cost?
Ah, this is so adorable XD
I could so wear that all year round lol.
I would totally buy them You don't sell them, do you?
Omg, I just discovered your gallery, and your hats are awesome! This one is especially beautiful. o_o
This looks AMAZING could i have one?
how did you do the ears?
how did you do the ears?
that's amazing!
can i has it?
Will you ever post a pattern on how to do the ears? or do you no longer have any time?
Wow!! that is amazing! I absolutely love that design! <3
luv it!!!!
OMG you could do a wolflink hat!
Aww! I want husky-hat for me at sled dogs racings!
It is wonderful
It is wonderful
i love ur hats.... im inspired 2 do one... hehe do u sew the eyes? how?
do yo have a pattern?
By that chart I use a small hemming stitch in matching thread and you can't see it.
Oh man, your hats are awesome :3
I kind of understand how it's done, but I can't reproduce it... Will you post the pattern soon? D: *Wants to make a hat for a friend*
ooh, nice
The grey and the blue compliment one another nicely.
The grey and the blue compliment one another nicely.
Its amazing and cute I love it!
woah, this is awesome
Oh wow o_o


text:
Question: What factors make materiality decisions complex and judgmental
What factors make materiality decisions complex and judgmental?
View Solution:
View Solution:
Answer to relevant QuestionsShould the materiality of misstatements be considered individually or combined to be considered in aggregate with other misstatements when considering whether the financial statements are misstated? When is a misstatement ...During the course of auditing year-end financial statements, the auditor becomes aware of misstatements in a company's financial statements. When combined, the misstatements result in a 4% overstatement of net income and a ...Assume the company's stock price goes down in a bear market that occurs at the end of the year. However, the stock price more than doubles in the next year. The company recognized goodwill impairment at the end of the year ...SEC Accounting and Auditing Enforcement Release No. 904 describes KPMG's 1993 audit of Structural Dynamics Research Corporation (SDRC). The AAER describes SDRC as a client that inflated revenues and earnings by recognizing ...Auditors need to determine appropriate benchmarks in setting overall materiality. What would be an appropriate benchmark for a profit-oriented entity or an entity whose debt or equity securities are publicly traded? A ...
Post your question


text:
Like 2 months or more ago I went to some party and I met a guy. We danced a little (he was kind of drunk) and then we kissed for some minutes. I just told him my first name (Sara) and from where i was (we are from different countries)
I though that i will never see him again but yesterday i received his friend request in Facebook! I ask him how he found me and he told me that he searched all the girls with my name and the country. It took him 2 months.
Now he wants a date.
Sorry my english!
Most Helpful Guy
Sounded like he must be obsessed with you and possibly desperate to see you again. Sure you can meet him again, just do it in the day time in a safe and secure location like a coffee shop, and possibly have a friend close by following you to make sure nothing goes wrong when you two meet. You still would have to be careful. But if you guys don't really click, it's time to move on and not remain in constant communication with each other.10|10|0
Thanks for MHO!


text:
Have you/ Will you get married for the purpose of fulfilling the one of many things that society had made mandatory of our human race? Or will you/ have you done it because you truly love that person and want to take it to the " next step". Let's be honest here, not everyone is meant for marriage, some people are perfectly fine with being in love without a marriage certificate for the rest of their lives.
Most Helpful Guy
I do not believe that there is anything about marriage and society that is mandatory. I decide what I want. With a partner, WE decide what WE want. It is all for our benefit, and nobody elses. Marriage is a status that was invented by man to pigeon-hole people. You were single and didn't have sex, or you were married and did.
There is absolutely no reason for a partner and I to get married just to conform to society's attempt to organise us. I am not religious, so there is no moral pressure on us either.
If I do ever marry, it will be because I love my partner and it will be her desire to be married.64|20|0
Thanks for your honesty😊
Always welcome. Take care, stay safe!! Thanks for MHO,


text:
Filter Results:
- Full text PDF available (0)
Publication Year
- This year (0)
- Last 5 years (0)
- Last 10 years (0)
Publication Type
Publication Venue
Brain Region
Learn More
- M Tohda, T Murayama, S Nogiri, Y Nomura
- Biological & pharmaceutical bulletin
- 1996
To clarify the quantitative and qualitative changes in type IV phosphodiesterase (PDE IV) with aging, phosphodiesterase (PDE) activity and [3H]rolipram binding in the cytosolic fraction from the brains of young and aged rats were examined. In all areas of the aged (100-week-old) rat brain except for hippocampus, the PDE activity was decreased by about half… (More)
- M Tohda, T Murayama, H Hasegawa, S Nogiri, Y Nomura
- Neuroscience letters
- 1994
To clarify the functional significance of type IV phosphodiesterase (PDEIV) in neurons and glia cells [3H]rolipram binding and phosphodiesterase (PDE) activity were examined in cytosol and membrane fractions of neuroblastoma N18TG-2 and glioma C6Bu-1 cells. [3H]Rolipram binding was highly observed in cytosolic fractions of N18TG-2 compared to C6Bu-1.… (More)
- ‹
- 1
- ›


text:
Joe shows us his medals and explains the advancement process in Special Olympics
Joe tells us about all the activities that he does in a normal week
Introducing Special Olympics Athlete Joe Feehily who will contribute a regular video blog on www.specialolympics.ie
I've been very busy recently with a Fashion Show in Galway, my Grandad's 90th Birthday and a Sale of Work at the Apple Tree Centre
UCD FC gave some tips to the coaches and players in the Eastern Region Ladies Football League
click on link to see how the league table currently stands
SO Unlimited is a radio show on Dublin City 103.2 FM which is totally dedicated to Special Olympics Ireland.
Volunteers are needed for the athletics event in CIT on Sat 28th May 2011.
A small number of volunteers are needed to support the Golf event in Fota Island Golf Club on Monday 30th May 2011.
The basketball Cup competition was held in the University of Limerick Arena and was a great success. Ten teams competed on the day from all over Munster. The event was supported by Vistakon Limerick.The volunteers made the day very pleasant for everyone involved, well done volunteers and well done all the athletes and their coaches.
Special Olympics Connaught
The Gateway Building
North West Business Park Collooney
E: email@example.com
Date: Saturday 13th May 2017
Venue: RosBowl, Roscommon Town
Time: 9.30am – 5.00pm
You can now sign up for events on line! click on me!
Annual Regional Meeting
Special Olympics Connaught Annual Regional Meeting (ARM) will take place on:
Thursday 27th April 2017 at 7pm
Athlone Springs Hotel, Monksland, Athlone
All registered athletes, volunteers and family members are invited to attend. We encourage clubs to send representation to the ARM as it is an ideal opportunity to see the growth & development that took place in 2016 and the plan for the year ahead.
We look forward to seeing you on the night and should you require further information please contact the regional office.
Telephone: 071-9118320
E mail: firstname.lastname@example.com
Special Olympics Connaught provides year round sports training and competition for over 1,300 adults and children with intellectual disabilities in Galway, Leitrim, Longford, Mayo, Roscommon, Sligo and Westmeath.
Over 30 sports competitions and development days are held annually in 13 Olympic type sports.
There are 73 Special Olympics clubs in the Connaught region.
The mission of Special Olympics is "to provide year-round sports training and athletic competition in a variety of Olympic-type sports for children and adults with an intellectual disability, giving them continuing opportunities to develop physical fitness, demonstrate courage, experience joy and participate in a sharing of gifts, skills and friendships with their families, other Special Olympics athletes and the community".
Thursday 27th April 2017 at 7pm
Athlone Springs Hotel, Monksland, Athlone
Regional Aquatics 2&3
Advancement Event
contact Annette for information
Regional Table Tennis
Advancement Event
contact John for information
Annual Collection Day May 5th
Contact Breffni on 071 91 18330 if you are available to help.
Bocce 7's League Winners 2015/2016
Westport Special Olympics Club
Rehabcare Castlebar
>> Special Olympics Sports
>> Become a Volunteer
>> Download Family Registration Form
>> Flickr- Connaught Photo Gallery
Annual Review
2015 Accounts
Strategy 2016-2020 - Full
Strategy 2016-2020 - Summary
Athlete Participation Form Guidelines
Club Handbook
Code of Ethics- long version
Code of Ethics- volunteers
Connect Newsletter
Event Management Guide
General Information Leaflet
Guide to Intellectual Disability
A Guide to Organising Inter Club Activities
Language Guidelines
Quick Guide to Competition
Regulations Governing Special Olympics Ireland Sport
Privacy Policy


text:
What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Can I export a form to be imported by another user account?Asked by iaguirre on April 15, 2013 at 07:47 AM
I have a free user account. I have designed some forms there.
Now I have another user account (Premium Yearly Suscription) and I want to export my forms to this account.
How should I proceed?
Thanks a lot.
Hi Nuria,
We can transfer your forms from your other account to your new account. Please provide us the following.
1. Old account username.
2. Form titles to be moved .
3. New account username.


text:
Your browser does not support iframes.
LOUISVILLE, KY – United States Attorney John E. Kuhn, Jr. today announced the guilty plea, from a Miami resident, before Chief Judge Joseph H. McKinley, Jr., to federal conspiracy and theft charges stemming from his participation in a warehouse burglary in Leitchfield, Kentucky, in March 2011.
LOUISVILLE, KY – United States Attorney John E. Kuhn, Jr. announced the guilty plea of Kathleen Marie Farias to charges related to the theft of over $243,000 from her Bullitt County employer, before Judge Greg Stivers in U.S. District Court.
LOUISVILLE – A Louisville man was charged this week by federal grand jury indictment, with two counts of theft of United States Postal Service (USPS) parcels, which had been left for collection with other mail matter, on December 3, 2015, announced U.S. Attorney John E. Kuhn, Jr.
Joseph L. Carr, 48, is charged with stealing a Hot Wheels Airbrush Auto Designer Kit from mail left at a home on Larchmont Avenue in Louisville, and stealing a coat, blanket, and rug from mail at another home located on Larchmont Avenue.
SHEPHERDSVILLE -- A man has been charged in the Sept. 30 robbery of a local convenience store.
Elesasar Morales has been indicted for robbery by the Bullitt County Grand Jury.
On that date, the indictment alleges that Morales went into the BP station at the Brooks Road interchange. While there, he used or threatened to use physical force against a woman in the store.
The action was a way to accomplish the theft of items from the store.
That is a Class C felony, which is punishable by 5-10 years in prison.
WASHINGTON – Contract therapy providers RehabCare Group Inc., RehabCare Group East Inc. and their parent, Kindred Healthcare Inc., have agreed to pay $125 million to resolve a government lawsuit alleging that they violated the False Claims Act by knowingly causing skilled nursing facilities (SNFs) to submit false claims to Medicare for rehabilitation therapy services that were not reasonable, necessary and skilled, or that never occurred, the Department of Justice announced today.
PADUCAH – Debra C. Pyfrom, the former manager of the International Brotherhood of Electrical Workers (IBEW) Local 816 Federal Credit Union, was sentenced yesterday in U.S. District Court by Senior Judge Thomas B. Russell to serve 78 months in prison and pay restitution in the amount of $600,520.16 for committing bank fraud, announced U.S. Attorney John E. Kuhn, Jr.
LOUISVILLE – The former Louisville teacher and assistant football coach, previously employed by a parochial high school, was sentenced today in U.S. District Court by United States District Judge David J. Hale, to 504 months in prison and a lifetime of supervised release, for violating child exploitation laws, announced United States Attorney John E. Kuhn, Jr.
LOUISVILLE – The former pastor of a Louisville, Kentucky, parish pleaded guilty before U.S. District Court Judge David J. Hale today, in United States District Court, to charges of violating federal child exploitation laws, announced United States Attorney John E. Kuhn, Jr.
Stephen A. Pohl, age 57, voluntarily surrendered himself to the custody of the United States Marshals Service today, and will remain in custody until sentencing before Judge Hale on March 29, 2016 at 1:30 pm.
SHEPHERDSVILLE -- A man who entered a guilty plea in late 2012 to torturing cats is now in trouble with the law once again.
Alex D. Phelps has been indicted by the Bullitt County Grand Jury on charges of forgery and theft by unlawful taking under $500.
The most severe penalty is a Class D felony, punishable by 1-5 years in prison.
However, due to the past felony conviction, any new sentence could be enhanced.
SHEPHERDSVILLE -- A Bullitt Circuit judge has accepted a guilty plea which will not require a former Shepherdsville employee to serve any time in jail.
Robert Bright's guilty plea on three counts of theft by unlawful taking under $10,000 was accepted during his recent final sentencing by Judge Rodney Burress.
The one-year sentence would be diverted for three years. If Bright does not commit another crime, he would not serve any time in prison.
Copyright © 2017
The Pioneer News Online is your source for local news, sports, events and information in Bullitt County and Shepherdsville, KY, and the surrounding area.


text:
Aerospace Engineers salary and career choices are given in short descriptions in the pdf file below. Please download here as you wish: Aerospace Engineer Salary And Career
The Pdf file contains 2 pages with key facts for different salaries and universities. The data depends on U.S. Bureau of Labor Statistics. We have analyzed and gathered all the information for you.
Aerospace Engineering and Aviation website provides information for universities, jobs, salary and museums for aeronautical, space and astronautical domain


text:
Prices, promotions, styles, and availability may vary. Our local stores do not honor online pricing. Prices and availability of products and services are subject to change without notice. Errors will be corrected where discovered, and Lowe's reserves the right to revoke any stated offer and to correct any errors, inaccuracies or omissions including after an order has been submitted.
James Hardie HardieTrim 3.5-in x 144-in Primed Arctic White Smooth Fiber Cement Trim
Item # 349809 Model # 967401
Image depicts product family/texture, may not represent color;contact your store for availability
Product Information
- Accentuate the natural beauty of your home with HardieTrim boards
- HardieTrim reduces time and money spent on maintenance compared to wood trims
- The smooth finish is great for a contemporary board and batten look
- James Hardie siding and trim are Engineered for Climate so no matter where you live, you can rest assured that your investment is going to stand up to Mother Nature
- Each substrate is precisely engineered to resist moisture intrusion, cracking, shrinking and swelling in the specific region for which it was designed
- Perfect for your next do-it-yourself project - pre-painted, easy to handle and install
- HardieTrim boards provide design flexibility for columns, friezes, doors, windows and other accent areas – adding punctuation to your design statement
- Fiber cement trim may cost less than wood trim, doesn't attract pests and is fire resistant
- HardieTrim boards are backed by a 15-year limited warranty
|Series Name
|Actual Thickness (Inches)
|Actual Width (Inches)
|Manufacturer Color/Finish
|Arctic White
|15-year limited
|Finish Warranty
|15-year limited
|Actual Length (Inches)
|Color/Finish Family


text:
I don't get it, why do people always tell me that my lips look nice but they have a hard time in believing me that they are actually all natural? I mean, do my lips not suit my face or do they look odd/bizarre? Then there are those guys who say I'm fake because apparently my lips look fake... like what's up with y'all? I really don't get it and I'm done with this whole 'issue'!
Most Helpful Guy
They are full, many people would pay for them. Why care about haters opinions. Don't listen to the less than 5% who tell you that. Most people don't care or love it.00|00|0
Most Helpful Girl
I guess they don't believe in you, because some girls are getting a surgery to get full lips. It's trendy now.
But, I do believe that some people have natural full lips.00|00|0
Hmmm that's true :/
Unfortunately it is.
But just because they have fake lips, it doesn't mean yours are too
Thank you for the MHO.


text:
Question: One of the potentially powerful advantages of m commerce is the
One of the potentially powerful advantages of m-commerce is the ability of sellers to target of fers to consumers when they are in close proximity to the seller. This is made possible based on smart phone technology that tracks the exact geographical locations of their users. For example, if a consumer is near a Staples Office Superstore, a text message can be automatically sent to the consumer's smart phone about a special offer on, say, print cartridges and paper. The technology that has made such m-commerce based proximity marketing possible was developed by startups such as ShopKick, Where, and Loopt, as well as by giants such as AT&T's ShopAlerts. Although available to anyone with a smart phone, the proximity alerts will only be sent to consumers who have sig nedup for the program. Do you think this type of m-commerce is an important channel option for consumers and sellers? Why or why not?
View Solution:
View Solution:
Answer to relevant Questions1-800 Flowers.com has been on Facebook almost from the first day the social networking site appeared. The online flower retailer uses Facebook to keep in touch with its many customers and to establish a forum to foster a ...Discuss the outlook for growth in franchise channels in the United States. Jose Fernandez recently became a franchisee in the Cartridge World franchise. Cartridge World specializes in selling replacement cartridges for printers and fax machine cartridges. Jose was pleased to be one of the almost ...Does the lack of a product flow alter the design and management of marketing channels for services as compared to marketing channels designed by manufacturers? Explain. Late one Thursday afternoon George Mitchell went to the airline ticket counter of a major carrier that had recently launched an ad campaign challenging customers to "find out how good we really are." Mitchell needed to ...
Post your question


text:
Organize your favourite listings
Sign up to keep track of your favourite spaces and revisit them later.
Contact Glen or David for more information about this suite.
Professional office space located directly across from Victoria Park in downtown London. Close proximity to Richmond Row and public transit. Onsite parking available at additional cost. Note: utilities included in additional rent charge. 496 SF office space available July 31, 2015. Wellington Street and Wolfe Street. Directly across from Victoria Park.


text:
Contact Us
Ask a Lawyer and Get Answers to Your Legal Questions
Ask an Expert
Ask a Lawyer
How JustAnswer Works:
Ask an Expert
Experts are full of valuable knowledge and are ready to help with any question. Credentials confirmed by a Fortune 500 verification firm.
Get a Professional Answer
Via email, text message, or notification as you wait on our site.
Ask follow up questions if you need to.
100% Satisfaction Guarantee
Rate the answer you receive.
Ask INFOLAWYER Your Own Question
Satisfied Customers:
Licensed attorney helping individuals and businesses
Type Your Legal Question Here...
INFOLAWYER is online now
I have a weird question. I sometimes get into the female
Resolved Question:
I have a weird question. I sometimes get into the female domination thing and was talking to a woman about a financial slavery
. She put it together and thinking it was more of a fetish theatrical contract I sent her the first payment. I never signed the contract and sent it to her anonymously. My question is this, if financial slavery legal and would a contract like that hold up in court? I can send you the contract if you want to take a look.
7 years ago.
Share this conversation
replied 7 years ago.
Slavery is illegal, sexual or otherwise. You can enter into an employment agreement. You should make sure your agreement does not amount to prostitution or imprisonment which could result in criminal charges.
Ask Your Own Legal Question
replied 7 years ago.
The following is the document. Again I never signed it, but thinking it was more of a theatrical document I paid the first tribute. Any thoughts would be appreciated...
Name: (not your new slave name)_____________________
E-mail address: ________________________________
Contact info: __________________________________
NF/Keen username: ____________________________
Position you are applying for:
_X_ Financial sub
What don't you enjoy? (Be specific. Nothing ruins a conversation like not knowing wtf to say). This is to keep from mentioning anything that will be considered as breaking the moment.
What are your fetishe [Princess Kassandra]s and turn ons? Please be specific here. If you like to be humiliated then tell me how i.e. small cock, being called a sissy, so on). Again this is simply to keep the moment going when we talk.
Payment: _X_ Weekly ____ Bi-weekly ____ Monthly
Payment days: _X_ Mon ____ Tues ____ Wed ____ Thurs ____ Fri ____ Sat ____ Sun
(All payments are to be paid no later than 8 p.m. EST on the due day, late payments will be subject to fines of $10.00 per day after due day until I decide to take further action, which you will be notified of when I decide what to do.
Amount to be paid $20 weekly
The details of this contract are as follows,
____________ will be named as Amanda and is the financial servant & property of Princess Kassandra; as OWNER of financial servant, Amanda & all money earned by him;
From: Jan 11, 2010 until Jan 10, 2011, Amanda has no right to make any changes to this contract, but Princess Amy can amend it at her will.
This contract states Princess Kassandra's rules for Amanda which he has to strictly & with strongest efforts to fulfill. Amanda is absolutely under obligation, without any failure or excuse, bonded to the rules of Princess Kassandra's contract. This contract is 100% real & binding.
The payments must be made by Amanda on a weekly basis & starting with a minimum of US$20.00 due on Monday, January 11, 2010 and $20.00 (U.S. currency) due each Monday by 8 p.m. EST.
On the 11th of each month the contract will be reviewed for increase of payment or stability of payment.
For each 24 hour payment delay, Amanda must pay Princess Kassandra a fine of US$10.00, in addition to his weekly payment.
If Amanda is more than 10 days late, he may be released from his contract & is subject to pay a release fee of US$1000.00 plus all fees owed before release. For instance, if Amanda does not pay his weekly fee for 10 days & is released, he will owe US$300.00 + US$1000.00 for early release from the contract + consequences that I deem fit at the time and will notify Amanda before actions are taken.
Princess Kassandra also has the right to control the complete pitiful finances & everything else in the insignificant life of Amanda. Amanda has absolutely no right to keep additional money for himself at all, such as gifts of his relatives, additional jobs, etc. All money received from any sources should be reported to Princess Kassandra. To be sure he is giving all that he has, she [Princess Kassandra] may demand real proof, such as statements, scans of bills, & check stubs. If XXXXX XXXXXes to Princess Kassandra, his fine will be US$100.00 & any fitting punishment that she [Princess Kassandra] deems necessary or funny.
If Princess Kassandra so chooses, SHE [PRINCESS KASSANDRA] can command Amanda to live modestly so that she [Princess Kassandra] can live frivolously & without worry or concern. IE: If Princess Kassandra commands Amanda to move to a craphole apartment so that she[Princess Kassandra] can have more money, then he will begin making arrangements to do so.
In his life as a financial servant for Princess Kassandra, Amanda must learn to manage with a modest allowance to be determined by her [Princess Kassandra] at a later date. He [Amanda] will receive a weekly allowance, because Princess Kassandra is so gracious. Every penny Amanda can save, is more money for Princess Kassandra to enjoy FRIVOLOUSLY.
Amanda understands that from this day forth, all of his money, what he earns with his meaningless jobs & all other income without exception belongs to the Princess Kassandra, & that it is only with HER permission that he is allowed to spend any of her [Princess Kassandra] money. Amanda must work hard, work
& work a second job to pay PRINCESS KASSANDRA more & more money. It does not matter how much Amanda earns, it belongs completely to PRINCESS KASSANDRA. Amanda as a financial servant & piece of property belonging to Princess Kassandra has no other rights than the right to pay large amounts of money to Princess Kassandra because he is nothing but an absolute pathetic, loser, source of amusement, financial slave & piece of property for Princess Kassandra.
When Princess Kassandra gives commands to her [Princess Kassandra] property, it has to obey without excuse. PRINCESS KASSANDRA has the full right to know what Amanda is doing at all times. PRINCESS KASSANDRA can call Amanda at any time or for whatever reason she [Princess Kassandra] desires. Amanda has to be always available for his owner. Should he not be available, he must return her [Princess Kassandra] call within 1 hour or suffer a STEEP fine and action to be determined by the PERFECT Princess Kassandra at that time.
Princess Kassandra can use Amanda for her [Princess Kassandra's] & other people's amusement. IE: She [Princess Kassandra] can demand that Amanda take special, humiliating, degrading and/or ridiculous photos of himself & send them to her [Princess Kassandra], so that She [Princess Kassandra] can post them on her website or where ever she [Princess Kassandra] may desire. Princess Kassandra may do this as punishment & sometimes just for a giggle. She [Princess Kassandra] alone has the full right to all photos of Amanda & Amanda agrees fully upon signing this contract.
This contract also mentions that Amanda call at least twice a week for a total of 4 minutes per call and must leave feedback and a high rating or provide a combination of purchasing something from Princess Kassandra's wish list and send a small contribution of $10 (U.S currency)
Princess Kassandra, Monday January 11, 2010 signed by (Amanda)
*Upon release from servitude, Amanda must pay a fine of US$1000.00 + all fees owed prior to release up to US$300.00. If he fails to pay this fine, Princess Kassandra will seek damages in
small claims
**This contract is auto renewed each month unless Amanda request release between 12 and 12:05 PM the last day of the contract.
2. This is not a joke or a game; do not treat it as though it is.
3. Do not act as though you are better than me.
4. You will go by your contract/application
5. Do not question any given task. My word is final.
6. All tasks should be done in a timely manner and by the deadline date given. No second chances will be given. DO NOT ASK.
7. If you do not complete a task or do as I instruct, you will be penalized and offered another task, if that is also not done, you will be considered dead to me and actions will be taken, determined by me.
8. Proof of tasks are required.
replied 7 years ago.
You should not sign it, make any payment further, or engage with this person further. It is illegal.
Ask Your Own Legal Question
replied 7 years ago.
What I did is illegal or this contract in general? Am I in legal trouble for this?
replied 7 years ago.
The slavery/property nature of the agreement renders it illegal. Both sides should walk away from it.
INFOLAWYER and 7 other Legal Specialists are ready to help you
Ask your own question now
Share this conversation
Related Legal Questions
Date Submitted
My question pertains to a Mover who stole prescription
I am a school administrator. The day before yesterday my car
Can I sue yahoo over my personal information possibly stolen
This is a small claims court case involving auto repair gone
Hi Zach, I have 2 concerns right now I hope you can help me
zdnlaw, wendy-Mod
My full-time employer, the owner of the company was my friend,
socrateaser, dkaplun
i loaned a friend over a thousand dollars. over a time span
I am a doctor with 4,000 patients and recently lost all of
I am with a company that provides event entertainment services
i dont have a copy of my contract with my previous employer
Ask a Lawyer
Get a Professional Answer. 100% Satisfaction Guaranteed.
7 Lawyers are Online Now
Type Your Legal Question Here...
characters left:
Contact Us
Terms of Service
Privacy & Security
About Us
Our Network
© 2003-2017 JustAnswer LLC
JustAnswer UK
JustAnswer Germany
JustAnswer Spanish
JustAnswer Japan


text:
It only takes a minute to get started. Fill out our simple sign up form and login to the site.
This step is voluntary but will drastically enhance your chances of receiving views from other daters on the website. Introduce yourself, your interests and upload your best photograph.
Utilise our website's advanced search to encounter over 60s members who satisfy your requirements. Or just browse through listings and encounter singles that appeal to you.
Discovered someone who you find interesting? Send a letter to them or just a wink and see if anything happens. With a little luck you'll be on your way to locating the love of your life.
It'll only take 30 seconds


text:
Toplist of Adult Games, 3D Sex Games, Flash Game Sites, Live Action Sex Games, Adult MMORPG, Virtual Strippers, Strip Poker, Blogs, Links, and Resources.
|Account Locked
If you're having trouble please contact us
Webmaster Programs
You may also be interested in these high converting affiliate programs I use. After a lot of experimentation I've found these to be the most reputable, and most profitable. All of these sponsors offer revenue share programs which pay the best over the long haul. All also have excellent customer service departments.
Promote Thrixxx Games: 3D Sexvilla 2, Hentai II 3D, and 3D Slut
Promote Adult Friend Finder: Biggest Sex Personals
Promote VirtuaGirl HD with TotemCash
Promote DreamStripper with AffiliatePro
Promote Virtual Hottie 2 with CCBill
0 Sites In Our Database
Last Update: 08-22-2017 06:20pm
© 2008-2009 The Virtual Sex Review
Your account has been locked by the administrator and cannot be edited. Contact Us
0 Sites In Our Database
Last Update: 08-22-2017 06:20pm
Home | Create Account | Login | Banners | Top Hentai | Sex Games Report | The Virtual Sex Review
Last Update: 08-22-2017 06:20pm


text:
"Disgusting treatment !"
About: Croydon University Hospital Croydon University Hospital Thornton Heath CR7 7YE
Posted by Marie
I am totally disgusted how i have been treated by your hospital ! I went to A&E on boxing day with blood and clots in my urine and retention unable to pass urine and severe pain. The Dr wanted to send me home as no infection found but an obvious problem,anyone could see that ! I was put on a catheter with no forwarding appointments until a lovely nurse spoke to the Dr and she felt i should be admitted. Anyway i was admitted to Queens 1 for 5 days with no tests apart from the urology team saying i needed an urgent cystoscopy but would have to be done as an outpatient as they were so busy ? I questioned the Dr and heard 15 minutes of him rattling on about hospital finances !!! Anyway i was discharged with a catheter bag and told to wait basically.Despite the promise of an urgent two week outpatient cystoscopy 15 days later i still hadn't heard a thing. I went to the ward and i demanded to speak to the urology team who told me they were sorry but someone accidently booked the procedure as an inpatient and i was forgotten after my discharge ! I went straight to Pals and they helped sort out an appointment for me ,a wonderful team. I finally had the cystoscopy done two weeks ago,the Dr noted i had some inflammation in my bladder but put it down to the catheter despite bleeding and pain before ! I was told i could have an obstruction further up in my ureter but he left me with no further investigations to be done and a follow up for 6 weeks time still connected to the catheter bag ! At present my catheter bags are two weeks out of date and the lancaster suite treated me in a rude vile way when i approached them for more bags and advice.The urology outpatients refused to help me also it's a disgrace,i have Lupus which opens me up to infection especially when it comes to the kidneys ! I am now considering making plans to take legal action if nothing moved on with my health care!
Response from Croydon University Hospital 3 years ago
Thank you for taking the time to share your experience with us, both here on NHS Choices and with our PALS team. It is clear that you did not receive the high quality, compassionate care which we aim to provide to all of our patients; we have passed your comments on to the teams that treated you and we will look in to the issues that you have raised. We are glad that you had a good experience in your dealings with our PALS team; please do get in touch with them on 020 8401 3210 or through email@example.com if you would like to be kept informed of the progress of our investigations.
- {{helpful}} of {{total()}} people think this response is helpful
- Was this response helpful? Yes | No
Thank you for your feedback.
See more responses from Croydon University Hospital


text:
Question: Suppose that z the marginal product of efficiency units of
Suppose that z, the marginal product of efficiency units of labor, increases in the endogenous growth model. What effects does this have on the rates of growth and the levels of human capital, consumption, and output? Explain your results.
View Solution:
View Solution:
Answer to relevant QuestionsSuppose that the government makes a onetime investment in new public school buildings, which results in a one-time reduction in consumption. The new public school buildings increase the efficiency with which human capital is ...A consumer receives income y in the current period, income y' in the future period, and pays taxes of t and t' in the current and future periods, respectively. The consumer can borrow and lend at the real interest rate r. ...Suppose in our two-period model of the economy that the government, instead of borrowing in the current period, runs a government loan program. That is, loans are made to consumers at the market real interest rate r, with ...Use the social security model developed in this chapter to answer this question. Suppose that a government pay-as-you-go social security system has been in place for a long time, providing a social security payment to each ...Suppose that there is a shift in the representative consumer's preferences. Namely, the consumer prefers, given the market real interest rate, to consume less current leisure and more current consumption goods.(a) ...
Post your question


text:
Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
I plan to bring two witnesses to my DUI case to prove that how many drinks I had on that one night. One of my witnesses want to know if she will get into trouble?
The only way I can imagine she would get in trouble would be if she perjured herself or testified to some fact that would incriminate herself.
The bigger concern I see from your question is that you are attempting to defend yourself on a DUI charge. I strongly suggest that you contact me or some other capable criminal defense attorney to represent you in this matter.
The information contained in this message is for general informational purposes only. It is not intended to be legal advice, nor is it a substitute for professional legal services. Wolcott Rivers Gates disclaims the formation of an attorney-client relationship by use of AVVO, and no such relationship exists unless and until a written Fee Agreement has been endorsed by Wolcott Rivers Gates and the client. Furthermore, given the public nature of this forum, there can be no expectation of privacy, confidentiality, privilege, or any other protection from disclosure.Ask a similar question
Your witness could possibly be charged with aiding and abetting a DUI. I've seen vindictive prosecutors do that.
I'm not sure I see the wisdom in having a witness testify you drank alcohol in a DUI case. You have to be careful. Some jurors hold the belief that its illegal to drink anything and drive although that is not the law yet they may hold you to it.
If your defense is that the BAC result is inaccurate you need expert witnesses to attack the accuracy of the test. If you do not have an experienced DUI attorney, get one.
This answer is my personal opinion, offered for informational purposes only. It is not a legal opinion, nor is it legal advice, nor does it create an attorney-client relationship with anyone reading it.Ask a similar question
Not sure why she will get in trouble?
Brook M. Thibault Law Offices of John D. hooker, Jr. & Associates 1206 Laskin Rd. Suite 110 Virginia Beach, VA 23451 TEL (757) 425-9977 FAX (757) 491-1978 Please note: Use of this website/ Q&A portal does not create an attorney-client relationship between users and any other party. Neither transmission nor receipt of information from this website/portal creates an attorney-client relationship between users of this site and any other party. No attorney-client relationship exists between the users of this website/portal and any other party. No communication or information contained herein is is to be legal advice.Ask a similar question
Sign up to receive a 3-part series of useful information and legal advice about DUIs.
Post a free question on our public forum.Ask a Question
- or -
Search for lawyers by reviews and ratings.Find a Lawyer


text:
Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
I have a c corporation that incorporated in Delaware, and I have an online startup company that haven't started selling services.
I am planning to move to California very soon. In the mean time can I sign contracts with clients without filing for foreign entity in either Pennsylvania or California?
I don't want to file for Foreign entity since I will be moving to California in 3,4 months, so I see it as a waste of money and will make things more complex...Or I don't even need to file for foreign entity although I am headquartered in Pennsylvania?
In other words, in which state/county as I being considered doing business in?
So if I want to protect my business name further should I file for DBA in that state only ( I already filed for Federal ServiceMark) ?Forgot to mention that website launch will start after moving to California. In the mean while still in pennsylvania i will be selling pre-orders to clients, signing contracts, etc. Those clients can be in any state, not just pennsylvania. Thank you
If you are operating a business headquartered in Pennsylvania, selling orders and signing contracts with individuals or companies in Pennsylvania, you are "doing business" in Pennsylvania. Regardless of your plans to move to another state, it sounds like you are currently doing business in Pennsylvania. While the PA statute does not explicitly define what it means to do business in the state, it does list some exceptions — activities that do NOT constitute doing business in Pennsylvania, including: "Soliciting or procuring orders, whether by mail or through employees or agents or otherwise, and maintaining offices therefor, where the orders require acceptance without this Commonwealth before becoming binding contracts." Accordingly, if your orders/contracts are strictly with non-Pennsylvania residents or entities, you are likely NOT doing business in Pennsylvania. The corollary to that exception is, however, that if you are making sales and signing any contracts that are completely within the boundaries of Pennsylvania, you are doing business in Pennsylvania. If that is the case, you should register your foreign corporation to do business in Pennsylvania.
Generally, foreign corporations cannot operate in Pennsylvania without a Certificate of Authority. The Application for a Certificate of Authority is a 2-page form that must be submitted with a $250 fee. Failure to obtain this Certificate will deny the foreign corporation access to Pennsylvania courts for bringing suit, though the corporation will still be subject to suit in Pennsylvania. The restriction on bringing suit, however, is not permanent — once you register in PA, you can sue in PA. Also, failure to register the foreign corporation does not invalidate the contracts you are signing (they are still good, valid contracts). With that in mind, you may be able to wait and see if you'll need to resort to the courts before you move to California. If you do, you can file your Application for a Certificate of Authority then. If not, you may be able to save the time and expense of doing so, and wait to register your foreign corporation in California alone.
In summary, based upon the facts you have provided, it seems that you are, indeed, doing business in Pennsylvania. Accordingly, you should file for a Certificate of Authority to do so. However, it is possible that, depending on the specifics of your activities, you may fall under one of the exceptions and be deemed to not be doing business in Pennsylvania. In either event, you may register your corporation at a later time if you need to resort to Pennsylvania courts.
This response does not create an attorney-client relationship and is not intended to provide legal advice for your specific situationAsk a similar question
You are registered in DE so that piece is taken care of. If you have not begun doing anything with the business in the C corporation, you can probably avoid filing as a foreign entity in PA. The definition of "doing business" varies from state to state. If you are signing contracts in PA, and if you are living and working in PA, you are almost certainly doing business in PA. The prudent thing to do is to register as a foreign entity in PA.
As a practical matter, the consequence of failing to register tends to be preclusion from the courts as a plaintiff and a few other things. I say that without reviewing the statute, which I have not looked at recently. So though the proper thing to do is likely to register as a foreign corporation in PA.
Of course, to be certain of a response, one would need to gather more information about exactly what you are currently doing while in PA.Ask a similar question
Post a free question on our public forum.Ask a Question
- or -
Search for lawyers by reviews and ratings.Find a Lawyer


text:
Added: Thursday, October 31, 2013
Loading Pornstar Spa Zoey Monroe: Zoey gets an anal massage Video
Zoey likes her massage. If you havent seen her before. Check her out she is a beautiful Blonde girl with a sexy body and a great personality. She has cute little titties and a fat ass. You can tell she loved her meat because she was all over the masseuses' dick and eager to suck it all day long. But Zoey isn't happy with just sucking it either or putting it in her pussy. She wants it all in her ass.


text:
So I was...what is that in the corner of my eye? NO SERIOUSLY WTF IS THAT?
- Take 2*
Okay so I was looking online for Adventure Time profile pics, when I stumbled across these interesting pics:These seem to be storyboards from the episode Betty!! How's that for exclusive? It's kinda' rare for pictures like this to be leaked onto the internet. It's really interesting. ONLY 1 PERSON ON THIS EARTH HAS ANNOUNCED THESE PICTURES, Im the second!!!!!! I don't know what thes pictures or what scenes these will appear in but one things for sure and that it's Betty! If at anytime you find any suspicious looking pictures that may look like it's from Betty, send em' down the comments and if I find any, I will update this blog and upload them onto here. And if you just can't wait for the episode, here's a picture to keep you laughing:


text:
Avanir Pharmaceuticals (NASDAQ:AVNR) announced the approval of Nuedexta during the fourth quarter of 2010, and shares quickly approached the six dollar mark in the trading days immediately following the release, making the stock a clean double in a very short period of time.
Those who may have taken advantage of some suspect trading that saw the stock dip to a low of $1.31 during the last hour of trading before the after hours approval announcement enjoyed gains of well more than a double.
Since then, however, Avanir has announced a stock offering to raise money for Nuedexta's commercial launch and the share price has since retreated to where it stands today, which is in the mid-$3 range.
The volatility comes as no surprise to those familiar with the biotech sector, and especially for companies such as Avanir whose stock fall victim to 'bear raid' trading as we saw on the day of the announcement, but the question investors will ask right now is whether or not this is the time to buy, since shares have depreciated significantly off the highs.
Neudexta is a treatment for pseudobulbar affect (PBA), a condition characterized by spontaneous and uncontrollable laughing and/or crying, and is slated to be the first-line treatment for that condition since there is effectively no other approved product on the market at the current time.
Analysts have predicted that sales for Nuedexta will approach the $500 million mark on an annual basis, leaving plenty of room for appreciation of the AVNR share price as the product hits the ground and starts chipping away at that price target. It also leaves plenty of room to advance the pipeline without having to raise cash over the mid to long term.
Avanir may have also become an opportune takeover target for big pharmaceuticals, most of which are looking to boost their pipelines and revenue streams over the short term since many big-named drugs are coming off patent over the next couple of years.
With an approved product ready for market and a pipeline in tact, Avanir fits the bill just as well as some others, namely Amarin Corporation (NASDAQ:AMRN), whose shares are sliding, and Apricus Biosciences (NASDAQ:APRI), which has a fairly robust pipeline ready to go.
An already volatile sector may continue to become even more so with the global unrest and potential for crisis, but there's some stock slides that are presenting nothing more than an opportune buy time with eyes toward the future.
Avanir is one of those, in my opinion.
The current prices are attractive enough, but should the stock sink even lower, and it's a possibility having seen the suspect trading on announcement day, then the risk/reward becomes that much better.
Disclosure: I am long AVNR, AMRN.
Avanir Primed for a Rebound?
Avanir Pharmaceuticals (NASDAQ:AVNR) announced the approval of Nuedexta during the fourth quarter of 2010, and shares quickly approached the six dollar mark in the trading days immediately following the release, making the stock a clean double in a very short period of time.


text:
What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Account: 502 Bad Gateway Error is shown when forms are submittedAsked by nagsplayers on November 04, 2016 at 01:33 PM
Our customers are getting a 502 Bad Gateway Error when they try to submit any forms. In fact, the forms do get submitted to JotForm, but the customers receive the Bad Gateway error message after a long wait and we don't receive the notification emails either. This has been happening since about Oct 27. I can't even submit a question to JotForm Support when I'm logged in to the account, and am having to use another account right now to send this. Please help as soon as possible. In the meantime I am recreating our forms using a competing software, but I would prefer to keep them with JotForm.
The problem is with ALL forms connected to our nagsplayer account. Please help as soon as possible, as we are a community theatre group and we have a show on right now - this is getting the way of our bookings.
JotForm Support Manager
I just submitted a test with no issue:
On regards to the email problem, this is due to the fact that we changed setting in our email servers this caused that public services like Gmail marked us as spam.
To resolve the issue, please check you Spam folder and look for any message with @jotform.com domain and mark them as "Not Spam"
Sorry... I've had so many problems just trying to send a message to JotForm Support that I've duplicated things now so there are two of you responding.
While I waited for things to get sorted out, I recreated the form under another account, and that's the one you tested. This other account is not having problems at all. This is the form that WAS on our web page. It's a different JotForm account, and I can't even submit a request for support to Jotform from this account without receiving the error.
But as I said, I think I've got someone else working on it now.
JotForm Support
I have tested your form that is created on account nagsplayer and I was able to replicate mentioned issue. When I submitted your form form was stuck on "Please wait..." for few minutes and then 502 Bad Gateway message was shown.
I made 4 submissions and I was able to replicate mentioned issue each time. I was also able to find my submissions on submission page your form.
However it also seems that Notifications were not sent. Thee is no data in email history log on your account.
Considering that I was not able to replicate mentioned issue on my cloned form, I will escalate this issue to our developers. Once this issue is resolved we will inform you via this thread.
For now I would suggest you to use cloned form that you created on your other account.
Has there been any progress made on this? I have not heard anything since you submitted to the developers on November 4. We were getting close to exceeding the free PayPal allowance and I was going to pay the monthly fee to cover us for the last day of our show but it seemed stupid to pay for a service that wasn't working properly.
JotForm Support
There are currently two threads opened for this issue and both are assigned to a developer:
Both have been given the highest priority, however, there are no further updates at this time. As soon as they have had a chance to address your ticket, we will update this thread.


text:
American Express Platinum Card
- Card rivals offer high-end rewards, but there's a catchnwaonline.com
- Shoppers have many high-end credit card options, but for how long?The Globe and Mail
- Travel Like a Boss with American Express Centurion Lounge AccessForbes
- Runway shows by Opening Ceremony and Wiz Khalifa to headline Made L.A.'s June 9 and 10 event downtownLA TimesYucatan , Mexico , San Luis Obispo , Carrizo , Sydney
- Cara Delevingne, Diane Kruger, Zosia Mamet Party with The Cast of 'Hamilton'observer.com
- PHL Airport Getting Fancy, Exclusive Loungephillymag.comSeattle , San Francisco , Miami , Las Vegas , Houston
- American Express profit falls 10 percent, but tops estimatesseattletimes.com
- Two credit cards that extend benefits to authorized userscsmonitor.comSan Francisco , Dallas , Miami , Houston , New York
- We Can All Relate To The British Olympic Team's Hilarious Airport Mistakehuffingtonpost.com
- How To Pretend You're A Local On Your Next Vacationhuffingtonpost.com
- Three ways to make your time at the airport more bearablecsmonitor.com
- 6 Hacks for Scoring Seat and Room Upgrades Over the Holidayshuffingtonpost.com
- Five credit card rewards you should usecsmonitor.com
- Five new travel trends for 2015csmonitor.comColombia , Buenos Aires , Argentina , Americas , Gogo
- Thanksgiving travel tips: Avoid checked bags, weather delayscsmonitor.com
- Kings to Raise Championship Banner Tonightwestsidetoday.com
- Is paying extra for airport clubs worth the money?The Globe and MailRichmond , Australia , Asia , Silicon Valley , Hong Kong
Frequent names
Newstral's extraction of names of people and organisations from the world's news is completely automated. If you do not want your name to appear here, please contact us. We will be happy to look at your request.


text:
FFA charges Melbourne Victory, Western Sydney Wanderers over fans' street brawl
Football Federation Australia has charged Melbourne Victory and Western Sydney Wanderers over a street brawl between the team's fans before last Saturday's A-League match.
Two men were hospitalised after fighting between supporters from both clubs before and during the 1-1 draw at AAMI Park.
Flares, bottles and metal poles were used as weapons in the fighting which took place on Melbourne's Bourke Street.
The FFA announced on Friday it was charging both teams with bringing the game into disrepute and said the clubs would have until 5pm on January 7 to show cause why they should not face sanctions.
"FFA has advised the clubs that their public statements this week on the events of 28 December are regarded as inappropriate and damaging to the interests of the A-League," the FFA said in a statement.
"FFA has advised the clubs that the proposed sanction is the deduction of three competition points from each club.
"The sanction would be suspended until the conclusion of the 2013/14 season, subject to the proper conduct of supporters for that period."
The points deduction would be activated in addition to any sanction imposed at the time if there were further incidents involving supporters.
FFA chief executive David Gallop defended the unprecedented proposed penalty.
"The loss of points that have been won fairly on the pitch is a harsh penalty, but it's in equal measure to the enormous damage caused by the type of anti-social behaviour seen in Melbourne last Saturday," Gallop said in the statement.
"The troublemakers are hurting the club they claim to support. They need to understand that the consequences of their reckless conduct will be felt on the A-League competition table if trouble persists."
Topics: sport, soccer, a-league, melbourne-3000, vic, parramatta-2150, nsw, australia
First posted


text:
Annual 2016 Conference
The mission of preserving and protection Wyoming's agricultural lands and open spaces from invasive species and pests is steeped in tradition. The annual conference of the WY Weed and Pest Council, taking place for over 70 years, is the opportunity for the 23 Districts to conduct Council business and to provide high quality continuing education opportunities for those working in the field. All are welcome!
Thank You 2016 Conference Sponsors
Cody, WY --- October 31 - November 1
Platinum Sponsors: Dow AgroSciences, Bayer, Alligare, Rozol
Gold Sponsors: Helena, WY Weed & Pest Council's Area 2, CPS
Silver Sponsors: Heinen Brothers, Nufarm, Valent
Bronze Sponsors: PBI/Gordon Corporation, Motomco, Integrated Weed Control
If we missed you in Cody, we look forward to seeing you in 2017! More information to come, so stay tuned!


text:
This is Lala, she has blond hair and blue eyes, she is multi colored dress with mustard and white tights and Mary jane shoes, she has a ribbon belt and flower in her hair.
Lala is one of a kind and perfect for your little one to love.
18" tall, handmade.
Ready to ship
Eye color can be changed


text: Show Hide image Culture 25 March 2013 Studio Sittings: Photographing Royal Academicians A new show at the Leighton House Museum. Sign up for our weekly email * Turning my bicycle onto Holland Park Road, I'm greeted by the sight of cherry trees and the sound of light wind. It's on this quiet street that Leighton House Museum sits; the life-long residence of Victorian painter Lord Frederic Leighton. Despite the encroaching hum of Kensington High Street, Leighton House remains a fortress of calm. Like a blushing pomegranate, the home's benign, redbrick exterior masks an interior opulence which Leighton spent over thirty years perfecting. "Leighton House is inextricably linked to the life of its one and only occupant," writes the museum's senior curator Daniel Robbins. The Yorkshire-born artist was the son of a wealthy doctor and grandson of the physician to the Russian royal family. Leighton trained in the art academies of Berlin and Florence and spent much of his youth travelling extensively across Europe and North Africa, fluent in five languages upon returning to London in 1859. Shades of turquoise and cobalt dominate the house that Leighton built to serve as home, painting studio and showroom for his vast collection of fine and decorative arts. Works of his own share walls with those of his contemporaries; blue silk chaises sit beside fireplaces stuffed with peacock's feathers. His love of painted tiles and decor imported from "The East" is exemplified in The Arab Hall, a £7,000 extension (extortionate sums for the times) built to house Leighton's hoard of some thousand ceramic tiles source by remote collectors in Syria, Greece, and Turkey. A still, square pool of water sits at the centre of this domed antechamber. The home "made plain his obvious though unostentatious wealth," writes Robins; regularly featuring in the press and open once a year for public viewing. After falling into misuse following Leighton's death in 1896, repairs began in the 1980s and, following major restoration works from 2008 to 2010, the house is one again open to a curious public. Foremost in Leighton's later career was his role within the Royal Academy, to which he was elected in 1868 and served as president from 1878. "Leighton proved an extremely effective President," Robins tells us, "displaying great administrative and diplomatic skills in handling the Academicians and making particular efforts to draw into the institutions artist such as his friends G F Watts and Edward Burne-Jones, who were by temperament inclined to remain outside." It's fitting, then, that the current curators of Leighton House have recently hung an exhibition called Studio Sittings: Photographing Royal Academicians. Portraits of contemporary RAs taken by German photographer Anne Purkiss span 25 years and capture the likes of David Hockney, Peter Blake, Antony Gormley and Grayson Perry at work in their studios. Presented alongside are photographic portraits of Victorian era Academicians including Lord Leighton himself, designer George Aitchison (who dreamt up much of Leighton House), architect Alfred Waterhouse and immanent London sculpture Hamo Thornycroft. Studio Sittings is set back from the main home in Leighton's winter studio: a classic Victorian glasshouse built to maximise the poor natural light that plagues London's cold months. Through the sheer walls visitors can observe an extensive garden in which crocuses bloom white and purple and pigeons amble purposefully. The space makes for an airy viewing of the black and white photographs, which feel particularly appropriate set against a grey sky framed by the room's iron skeleton. It is a simple and pleasant exhibition who's most interesting (perhaps unintended) consequence is mediation on the nature of portraits set in studios. Each artist is pitched against a personalised yet carefully constructed "creative space", framed by their tools, their possessions, their half-finished projects. The effect is both naturalistic and highly stylised, a non-verbal announcement of an individual aesthetic timbre. The effect is even more pronounced in the photographs of the Leighton-era RAs, who stand with hips cocked against their finest canvases, brushes held aloft like rapiers, palettes like shields. Despite the presence of several prominent RA women (Elisabeth Frink, Elizabeth Blackadder, Mary Fedden) the show feels inherently masculine (the RA has yet to elect a female president) and it's hard not to ponder what gallant tones these artists seek to invoke. As art historian Marcia Pointon recently remarked in her excellent study of portraiture, the construction of identity is often "better apprehended by ignoring faces and attending to other parts of the body, and to the ways in which those body parts are covered and adorned." From manner of dress to matters of props, the studio portrait has become a vehicle for the projection of the self as artist, inventor and tinkerer, wielder of tools and master of materials. Wonder at this little exhibition, then step back and think what this great house, full of a thousand objects, will tell you about it's one and only occupant. Studio Sittings: Photographic Royal Academicians is on at Leighton House Museum, 12 Holland Park Road, London W14 until 2 June, 2013. *** (Lord Frederic Leighton PRA. Kensington, London c. 1890. Photo: Ralph Winwood Robinson © Royal Academy of Arts, London) (Henry Stacy Marks RA. London c. 1889. Photo: Ralph Winwood Robinson © Royal Academy of Arts, London) (George Frederic Watts RA. Compton, Surrey, c. 1902. Photo: Original glass-plate negative, Watts Gallery Archive) (David Hockney RA. Southbank, London, August 1985. Photo: Anne Purkiss) (Sir Anthony Caro RA. London, May 1991. Photo: Anne Purkiss) (Kenneth Armitage RA. Hammersmith, London, October 1995. Photo: Anne Purkiss) (Dame Elisabeth Frink RA. Wollwand, Dorset, March 1990. Photo: Anne Purkiss) (Antony Gormley RA. London, November 1994. Photo: Anne Purkiss) (Dame Elizabeth Blackadder RA. Edinburgh, September 2011. Photo: Anne Purkiss) (Sir Denys Lasdun RA. London, March 1997. Photo: Anne Purkiss) Charlotte Simmonds is a writer and blogger living in London. She was formerly an editorial assistant at the New Statesman. You can follow her on Twitter @thesmallgalleon. Subscribe from just £1 per issue More


text:
Shelly debuted in WWE's ECW brand on June 20, 2006 as Ariel, a mysterious tarot card reader. After doing a session with Kevin Thorn, Ariel aligned with Thorn, becoming a villainous vampiress and serving as Thorn's valet. In her role, Ariel feuded with Divas such as Kelly Kelly, Trinity, and even defeated ECW original Francine in a singles match. In 2007, Ariel became the lone female member of the New Breed faction, which consisted of Thorn, Marcus CorVon, Matt Striker, and Elijah Burke. After her release from WWE in May 2007, Shelly appeared at TNA's Bound For Glory months later and participated in the Gauntlet Battle Royal to crown the first ever Knockouts Champion. She later became a regular TNA Knockout known as Salinas, the babyface valet for the Latin American Xchange, a role she played throughout 2008.
Shelly debuted for Championship Wrestling From Hollywood in 2010, and later served as the babyface valet for Blake "30th Century" Fox, before he was revealed as Todd Chandler during his feud with Ricky Mandel. On August 3, 2013, Shelly appeared during Chandler's match against Mandel and threw in the towel, giving Mandel the victory. After the match ended, Shelly turned into a villainess and delivered a low blow to Chandler, dumping him in the process for Mandel. In the weeks that followed, the evil Shelly served as a valet for Mandel, while also continuing to antagonize Chandler. She even teamed with Mandel in a losing effort to Chandler in a handicap match.
Shelly has been mostly portrayed as an arrogant villainess as well as a seductive femme fatale, even in her stint in WWE as the vampiric Ariel.


text:
Blog Directory
Browse the Blog Directory
Browse blogs from wide range of categorized blogs on our blog directory. Whether you are looking for niche blogs, personal blogs, Sweden blogs, Recreation blogs you can find them all in our blog directory.
Submit your Blog
Or, Submit your blog and be included on our search engine friendly blog directory.
Sweden RecreationBrowsing Sweden Blogs » Recreation Blogs
Review the most popular blog sites about recreation and entertainment at BlogsDB. Add Recreation blogs and promote on our most trusted blog directory online.
Search within these results:
Ropcorn - a famous blog that caters many useful articles concerning life in Sweden, Swedish traditions, the author and her dog, personal blogs, pets and recreation altogether.


text:
Listen to free music played by earredondo
Blip.fm is internet radio made social. It's easy to search for, play, and discover free music recommended by real people. Join today to create your own free station and start listening to @earredondo.
earredondo U2 & Mary J. Blige - One
What other DJs are saying about U2 & Mary J. Blige One Love
BlueDemon ONE LOVE @ellendianne
LindyLuv ...one love...one life...I need ya darlin'


text:
BDNF Val66Met polymorphism and protein levels in Amniotic Fluid
Brain-Derived Neurotrophic Factor (BDNF) is a neurotrophin which plays survival- and growth-promoting activity in neuronal cells and it is involved in cellular plasticity mechanisms as it controls activity dependent synaptic transmission. A functional polymorphism (Val66Met) in the pro-region of BDNF, which affects the intracellular trafficking of proBDNF has been associated with memory and cognitive deficits as well as to an increased susceptibility for several psychiatric disorders especially those with a neurodevelopmental origin. To date, no study has evaluated the influence of the Val66Met polymorphism on BDNF levels in a peripheral system that may reflect fetal neurodevelopment. Therefore we investigated in amniotic fluids (AF) obtained from 139 healthy women during 15-17 week of pregnancy, BDNF protein levels in correlation with the Val66Met polymorphism.
Interestingly we found a significant BDNF protein levels reduction in 55 Met carriers (Val/Met and Met/Met) (p = 0.002) as compared to 84 non carriers (Val/Val), and no effect of fetus gender, maternal age or gestation week on BDNF levels has been observed.
These results, although explorative, indicate that during fetal life the Val66Met genotype might influences BDNF protein levels in AF supporting the involvement of this polymorphism in behavioral and functional brain individual differences in the adulthood.
Brain-Derived Neurotrophic Factor (BDNF) is a neurotrophin widely expressed in the brain, which regulates neuronal survival, growth and connectivity during development and participates in synaptic plasticity mechanisms throughout adult life
BDNF protein is first synthesized as a glycosylated precursor (proBDNF) that is then converted intracellulary to mature BDNF protein, the latter being stored and released upon cell stimulation
Furthermore, the Val66Met polymorphism has also been widely investigated as a genetic susceptibility risk factor for a large spectrum of neuropsychiatric disorders, in particular those with a neurodevelopmental origin
To date, at our best knowledge, no study has evaluated the putative influence of the Val66Met polymorphism on BDNF levels during pregnancy, a period during which fetal brain development appears vulnerable to external stimuli. In fact, it is well accepted that adverse events occurring during fetal life have profound and persistent effects on brain functions and may represent risk factors for psychopathologies later in life
Therefore the aim of this study was to analyze putative functional effects of the BDNF Val66Met polymorphism in the fetal environment investigating correlation between BDNF protein levels and genotype in a sample of AFs obtained at the 15th-17th gestational week of physiological pregnancies.
The BDNF fetal genotype distribution in our study population was 3.6% (5/139) for Met/Met, 36% (50/139) for Val/Met and 60.4% (84/139) for Val/Val. Genotype distribution had no deviation from Hardy-Weinberg equilibrium (χ2 = 0.55, p = 0.46, HWE program, John Ott version 1.10). Since all the studies that evaluated the functionality of this polymorphism assembled Val/Met individuals with Met/Met and it has also been demonstrated that the Met allele has a dominant effect, we performed our analyses subdividing our study-sample into carriers (Met66Met, Val66Met) and non carriers (Val66Val homozygotes) of the Met allele.
We firstly assessed whether maternal age or gestational week was significantly different in Met carriers versus non carriers, but we didn't find any difference (mean values ± SD: gestational age = 16.2 ± 2.4 in Val/Val and 15.9 ± 0.7 in Met carriers, p = 0.314; maternal age = 35.4 ± 3.7 in Val/Val and 36.2 ± 3.1 in Met carriers); there was also a similar fetal gender distribution among carriers and non carriers of the Met allele (49.1% of females in Met carriers and 44.0% of females in non carriers).
We subsequently evaluated BDNF levels in AFs and we found that BDNF protein levels were significantly lower in Met allele carriers when compared to non carriers. In fact, as shown in Figure
Figure 1
BDNF protein levels distribution in 139 AFs (84 Val66Val individuals and 55 Met66 carriers)
BDNF protein levels distribution in 139 AFs (84 Val66Val individuals and 55 Met66 carriers). BDNF protein levels, evaluated by ELISA method, are significantly reduced in Met carriers versus non carriers The amount of BDNF expressed in pg/ml was 10.02 ± 9.62 for Met allele carriers and 15.88 ± 12.71 for non carriers (Mann-Whithney test: p = 0.002). In the group of Met allele carriers, the Met66Met are evidenced in black. Error Bars indicates the mean of values ± standard deviation.
This study represents the first evidence of an effect of the BDNF Val66Met polymorphism on protein levels during the 15-17 weeks of pregnancy.
Egan and colleagues
Furthermore, studies analyzing gene-environment interactions
It is well known that human brain undergoes complex morphological and functional changes during fetal development. In particular, during the first and second trimester of pregnancy, cortical subplate reaches its peak of development, and cortical neurogenesis, characterized by proliferation, migration and organization of neuronal connections, is predominantly taking place
In parallel, circulating BDNF levels in fetus could reflect those in the brain since a correlation between blood BDNF levels and the cortical protein expression has been observed during neurodevelopment in rats
Some limitations of our study have to be mentioned. The data about AF BDNF levels are from one point in time, and the relationship between BDNF genotype and AF BDNF levels may be different at other times during gestation. Additionally, this study is correlational in nature and we cannot draw any definitive statements about the consequences of BDNF Val66Met genotype on AF protein levels. Larger studies in wider and better characterized samples are needed to confirm these findings.
In conclusion, this study represents the first evidence of the functionality of the BDNF Val66Met polymorphism in the fetal environment showing a correlation between the genotype and BDNF protein levels in AF during neurodevelopment. Although these results are explorative, they support a role of this polymorphism in the individual differences in brain morphology and function, and in mental disorder susceptibility.
Sample collection
All the 139 amniotic fluid samples used in the study were obtained by amniocentesis performed between the 15 and 17 weeks of pregnancy (mean of gestational week: 16.1 ± 1.9) for fetal karyotype analysis requested by mothers for their advanced age (mean of maternal age = 35.7 ± 3.5). The inclusion/exclusion criteria were: healthy women with Caucasoid origin, fetuses with 46 XX or 46 XY karyotypes, no infections or complications during the previous gestational weeks and during the amniocentesis. The fetus gender was so distributed: 73 males and 61 females.
After amniotic fluid collection, each sample was centrifuged: the supernatant (AF) not necessary for alpha fetoprotein dosage was taken anonymously and stored at -80°C. Pellet fractions (AF cells, AFc) were cultured firstly in Amniochrome medium, which was replaced after 6-7 days with RPMI supplemented with 2 mM L-glutamine and 20% of Fetal Bovine Serum (FBS) as for routinely karyotype analysis. The leftover of AFc not necessary for karyotype analysis was collected anonymously without name or other detail, but with a number and bar-code. The study protocol has been approved by the local ethic committee (CEIOC).
DNA purification and genotyping
DNA was isolated from AF cells using TRIZOL reagent, according to the manufacturer' protocol (Invitrogen) and was quantified by Spectrophotometer (NanoDrop Technologies, Delaware, USA). Primer pairs used to amplify the sequences containing Val66Met were: BDNF forward primer: AGGTGAGAAGAGTGATGACC and BDNF reverse primer: CTGGACGTGTACAAGTCTGC.
The PCR reactions were performed in a final volume of 25 μl with 2 μl of genomic DNA (100 ng/μl), 0.1 μl of each primers pairs (100 μmol/ul), 0.7 mM of each dNTPs (Eppendorf), 0.5 μl of MgCl2 2 mM, 1.25 units of Taq Polymerase (Bioline) and buffer supplied by the manufacturer (Bioline). The PCR products were then sequenced using an ABI BigDye Terminator Cycle Sequencing Kit and by 3130 XL DNA Sequencer Analyzer (Applied Biosystem).
BDNF determination in Amniotic Fluids
BDNF levels in AF samples (n = 139) were measured by ELISA method using the human BDNF Quantikine kit (R&D Systems, Minneapolis, USA). The BDNF content was expressed as equivalent of human recombinant BDNF protein (pg of BDNF protein/ml of AF). The minimum detectable dose of the assay is typically less than 20 pg/ml.
Statistical analysis
Analysis was performed using Statistical Product and Service Solutions 13.0 Version (SPSS). Results are given as mean value ± standard deviation. The Mann-Whithney test was used to evaluate differences in quantitative variables. The Pearson coefficient was used to evaluate bivariate correlations.
Authors' contributions
AC Study design, data analyses and manuscript writing; LB-C Study design, data analyses and manuscript writing; RZ, SM, biochemical analyses; FC, EM genotyping analyses; EM, DB sample collection and clinical characterization; SB, MAR and MG Study design and manuscript critical revision. All the authors have contributed and approved the final manuscript.
Funding for this study was provided by Italian Ministry of Health (Ricerca Corrente) and by Cariplo Foundation.
The role of neurotrophins in axonal growth, guidance, and regeneration
BDNF and activity-dependent synaptic modulation
Genetic modulation of BDNF signaling affects the outcome of axonal competition in vivo
Nerve growth factor, brain-derived neurotrophic factor, and neurotrophin-3 levels in human amniotic fluid
Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF
The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal function
Variant Brain-Derived Neurotrophic Factor (BDNF) (Met66) alters the intracellular trafficking and activity-dependent secretion of wild-type BDNF in neurosecretory cells and cortical neurons
Dose-dependent effect of the Val66Met polymorphism of the brain-derived neurotrophic factor gene on memory-related hippocampal activity
Effect of BDNF Val66Met polymorphism on digital working memory and spatial localization in a healthy Chinese Han population
Brain-derived neurotrophic factor val66met polymorphism affects human memory-related hippocampal activity and predicts memory performance
The BDNF Val66Met polymorphism impacts parahippocampal and amygdala volume in healthy humans: incremental support for a genetic risk factor for depression
Neuronal correlates of brain-derived neurotrophic factor Val66Met polymorphism and morphometric abnormalities in bipolar disorder
Meta-analysis reveals no association of the Val66Met polymorphism of brain-derived neurotrophic factor with either schizophrenia or bipolar disorder
Genetic association study of BDNF in depression: Finding from two cohort studies and a meta-analysis
Brain derived neurotrophic factor gene Val66Met and -270C/T polymorphisms and personality traits predisposing to anorexia nervosa
Genetic analyses of the brain-derived neurotrophic factor (BDNF) gene in autism
Association study of brain-derived neurotrophic factor (BDNF) and LIN-7 homolog (LIN-7) genes with adult attention-deficit/hyperactivity disorder
Variation in brain-derived neurotrophic factor (BDNF) gene is associated with symptoms of depression
BDNF gene: functional Val66Met polymorphism in mood disorders and schizophrenia
BDNF Val66Met variant and age of onset in schizophrenia
Brain-derived neurotrophic factor Val66Met polymorphism: association with psychopathological symptoms of schizophrenia?
Imaging of early brain injury and cortical plasticity
Stress during development: Impact on neuroplasticity and relevance to psychopathology
Impact of genetic variant BDNF (Val66Met) on brain structure and function
BDNF Val66Met polymorphism significantly affects d' in verbal recognition memory at short and long delays
Impact of the brain-derived neurotrophic factor Val66Met polymorphism on levels of hippocampal N-acetyl-aspartate assessed by magnetic resonance spectroscopic imaging at 3 Tesla
Research applications of magnetic resonance spectroscopy to investigate psychiatric disorders
The BDNF Val66Met polymorphism affects amygdala activity in response to emotional stimuli: evidence from a genetic imaging study
A brain-derived neurotrophic factor (BDNF) haplotype is associated with antidepressant treatment outcome in mood disorders
Interactions between BDNF Val66Met polymorphism and early life stress predict brain and arousal pathways to syndromal depression and anxiety
The BDNF Val(66)Met × 5-HTTLPR × child adversity interaction and depressive symptoms: An attempt at replication
Proteomics analysis of human amniotic fluid
Postnatal developmental profile of brain-derived neurotrophic factor in rat brain and platelets
Association study of the decreased serum BDNF concentrations in amnestic mild cognitive impairment and the Val66Met polymorphism in Chinese Han
BDNF Val66Met polymorphism is associated with unstable angina
The Met allele of the BDNF Val66Met polymorphism is associated with increased BDNF serum concentration


text:
Air Jordan 2 Low "UNC" & Air Jordan 9 Low "Pantone"
Posted on June 07 2016
Air Jordan continues its Air Jordan Retro low takeover with its upcoming release of the Air Jordan 2 Low "UNC" and Air Jordan 9 Low "Pantone". Both covered in a white/ university blue/ navy color palette these Air Jordan Lows both add the perfect footwear to add to your closet this season.
The Air Jordan 9 Retro Low "Pantone" comes with a University Blue dominated upper that resembles the exact shade of blue used for the UNC tarheels. This shoe features a white midsole and Tarheel colored Jumpman logo on the bottom of the sneaker.
The Air Jordan 2 Low "UNC" comes in an almost completely covered white upper, contrasted by navy and university blue accents. Featuring a Navy blue heel cup and university blue/navy air jordan logo to stamp the tongue of the sneaker.
The Air Jordan 2 Low "UNC" ($160 USD) and Air Jordan 9 Low "Pantone" ($170 USD) will release this Saturday, June 11th at all Social Status locations.