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MMOs.com is a leading portal for MMOs and MMORPG reviews, previews, news, editorials, and more. Our goal is to catalog every massively multiplayer online game and provide visitors with a quick snap shot of these games with full reviews, screenshots, and videos. We aim to provide fair and honest reviews for all the games we cover.
And we feature editorials/columns covering every aspect of the industry, from conceptual analyses to interviews with development studios.
We are currently seeking writers who are eager to contribute columns on any topic related to MMOs. Some examples include "Black Desert's Buy To Play Model Wont Work," and "The Most Memorable MMORPG Soundtracks." We are interested in covering a wide range of topics and the full list can be viewed by visiting mmos.com/editorials.
About The Position:
Contributors will write about aspects of the industry they're passionate about exploring. We don't want to dictate your work, it should stem from a natural curiosity because you're enthralled with MMOs and its culture. But our editor will work closely with you to brainstorm ideas if you like, and we have a long backlog of editorials to be written.
~500 word minimum per contribution, and there is no maximum word count. You can see an example of a smaller post here: "MMO Hopping Is Incredibly Common," (~471 words). But other posts such as a recent review of the Steam Controller have ranged into the thousands. We want you to take as much space as needed to elucidate your point. But we do treat brevity seriously and you will work with our editor to refine your article.
You will work closely with our Editor. All works are edited with thorough feedback and explanations from our editor. We treat the editing process as a discussion and contributors are free to dispute any change they feel unwary or unsure about. We want to work with writers who are willing to learn and share a passion for both writing and MMOs. But no article is published without the final consent of the author.
Editorials must be exclusive and not copied from a personal blog or other website. We don't want writers to submit verbatim articles they have already written for another website or their own personal blog.
The position is non-paid but we are a young site that is quickly growing and the potential for full time employment is possible in the future, based on contributions and quality of work. We can offer link-backs to any personal blogs, social media profiles, and exposure to our rapidly growing audience, through social media postings and front-page display on the site.
Contributors Should Have:
–Passion for MMOs and the industry
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If you're interested in working with us you can contact our editor Sean by emailing him at firstname.lastname@example.com. Please include a writing sample that demonstrates your command of the English language (it doesn't have to be related to the industry but is preferred).
We look forward to hearing from you! ^_^


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There is currently unprecedented investment and renewal underway in Fremantle with the combined level of private and public investment in the pipeline totalling more than $1.3 billion including the once-in-a-generation Kings Square cultural precinct redevelopment. As part of this, the City of Fremantle has set ambitious targets for the development of new dwellings, commercial office and retail floor space within the city's central area to be achieved over the next decade.
Invest Fremantle prospectus
To learn more about investing in Fremantle, view our Invest Fremantle prospectus, or contact the economic development team for a hard copy.
Investment update
Fremantle's evolution is happening now, see some of the recently completed developments in our latest video about Port City priorities - from oval to ocean.
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Amidst the high levels of development activity, opportunities to secure strategic development sites still exist, please contact the City's economic development team for more information.
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subset: commoncrawl


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What Goes Under The Cake Layers?
Decorating By justbyangela Updated 22 Oct 2009 , 4:00pm by kakeladi
This may sound like a silly question, however I'm new and I need all the help I can get so please bare with me!
What exactly do you put under each cake tier? I bought a pack of about 5 cake boards. Do I cover these in aluminum/wax paper? Or is it something else under each of the tiers? I am making a two tiered cake and I have a base for the bottom tier. I am just wondering what I would do for the top tier? Would I just place it on the bottom tier without anything under it or do I need to place it on top of a cake board as well?
You want to place the top tier of cake on a cake board. You should also have some kind of supports that go into the bottom tier to keep the top tier from sinking in to the bottom.
Here are wiltons instructions for stacking a cake:
Good luck and welcom to cc!
I put the top tier on a cake board the same size. Before I put it on the other cake, I put dowels in to support the weight. Then I just put the other cake on top. I'm not sure if that's right but I haven't made many tiered cakes. I'm new-ish too.
just a plain cake board will do--you don't need to cover it in anything. Then bubble tea straws, or whatever you are going to use as supports underneath the top tier.
Check out SPS (Single Plate Support) - it's easy and the best way to stack a cake IMHO. There is a sticky about it in the "how-to" forum section. Each tier needs a cake board under it to support it, just like cupcakemkr said. You can buy pre-cut cardboard cake boards from Wilton or you can cut your own to size and shape using foamcord, which is what a lot of us do. Foamcord is extremely sturdy and lightweight and won't buckle under the weight of a cake like cardboard does. Many of us cover the foamcoard in "Press 'n Seal" wrap before placing the cake on it.
Your bottom board, the one that the final cake sits on to make it look presentable, etc. can be covered in a special foil specifically for cake boards (comes in gold and silver mainly) or you can cover the board in fondant to make the rest of the cake. The around the edges you can wrap a pretty ribbon around it finish the look. I would suggest getting a how-to cake decorating book with basic instructions in it to help you out. HTH and welcome to CC!
I prefer to cover every cake board I use. That covering can be 'regular' household foil; freezer paper; foil or mylar wrapping paper; a plastic tablecloth; or any other item you can think of that will not soak up grease &/or moisture
I never put a cake directly on any cake board w/o covering it 1st.


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Question: What does a 2D protein gel show How can the
What does a 2D protein gel show? How can the results of such a gel be correlated with protein function?
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View Solution:
Answer to relevant QuestionsApart from genome size, what factors make complete assembly of a eukaryotic genome more difficult than assembly of a prokaryotic genome? How does regulation by sRNA differ from that of riboswitches? What are the two components that give their name to a signal transduction system in prokaryotes? What is the function of each of the components? How would you design a regulatory system to make Escherichia coli use succinic acid in preference to glucose? How could you modify it so that E. coli prefers to use succinic acid in the light but glucose in the dark? Describe the events that occur on an agar plate containing a bacterial lawn when a single bacteriophage particle causes the formation of a bacteriophage plaque.
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Trump Taj Mahal Re-Investment Won't Happen If Casinos Allowed In North Jersey, Billionaire Says
Casino's New Owner Waiting On Results Of Likely November Referendum
This could be bad news for poker players hoping for a re-opening of the Trump Taj Mahal's poker room.
Billionaire Carl Icahn told the Associated Press Wednesday that if Garden State lawmakers and state voters allow casinos in north Jersey he won't be spending $100 million on upgrades and other enhancements to the Taj, which he recently acquired for less than $300 million thanks to the casino's Chapter 11 bankruptcy.
The Taj closed its poker room early last year and hasn't yet re-opened it. It had originally planned to re-open the storied room last summer. With 48 tables, the Taj's poker room was once the second largest in the city behind the Borgata.
"Although I had planned to invest up to $100 million in the Taj, just as I made substantial investments at the Tropicana, obviously it would not be judicious to proceed with those investments while gaming in north Jersey is an open issue, and we will have to wait to see the outcome of those proposals," Icahn told the AP.
Icahn did say he will pump between $10 million and $20 million into the casino for maintenance purposes regardless of the north Jersey casino decision.
At this point, a November referendum on allowing casinos outside of Atlantic City seems like a foregone conclusion. Atlantic City currently has eight casinos, but more are expected to close if north Jersey gets Las Vegas-style casino gambling.
over 1 year ago
I'm not surprised that Mr. Icahn would oppose North Jersey casinos, but I don't understand his silence on the boutique casino bill that would add 2 small casino in Atlantic City. It's time for some organization to study the probable impact of a casino at the Meadowlands on AC and on regional casinos in PA and NY; because that is the one location that could be operational, very quickly after a Statewide referendum. The convenience factor has already helped our previous market grow by 31%, from $5.2 billion to $6.8 billion. Personally, I would like to see the North Jersey bill add the 55% slot tax, as proposed by Jeff Gural, along with a 14% to 16% table win tax; because this would produce maximum taxes for New Jersey; plus I don't think another destination casino could justify a multi $billion project at these tax rates. And if Atlantic City were to get a third or more of the new tax revenues, AC projects could receive as much as $150 million a year; possibly to cover some of the City's real estate tax losses from casino revaluations. And some could be available to subsidize the start up of air service into AC International, which would enable South Jersey to promote Nationwide conventions and trade shows. AC could be a mini Vegas, depending more on other department earnings, than from the casino. Studying the Nevada Abstracts for 2014, the latest data available, will show the 23 largest Strip casino/resorts earning $2.41 billion in the rooms department and only $1.98 billion from the casino; with the food, night club and other departments earning another $1.94 billion. So the casino department produces 36.6% of the revenue, but only 31.2% of overall departmental income. And in 2015, casino revenues on the Strip fell even further, to 34.9% of overall revenue.
Over the past 9 years, AC casino revenues have declined from $5.2 billion to $2.5 billion; but looking at the population of our primary markets in 2006, roughly 95% now have a casino much nearer home. So we are still drawing from our old customer bases, but with less frequency and rarely mid week, where the convention trades book, possibly AC's best opportunity for the future. Prior to 2006, we boosted out mid week casino play with line run busses, at one time reaching 14 million persons annually. But we have lost nearly 90% of those customers, many now going from Manhattan to Sands Bethlehem. Steven Norton
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text: The following commentary comes from an independent investor or market observer as part of TheStreet's guest contributor program, which is separate from the company's news coverage.NEW YORK ( ETF Expert) --Greece might not take the bailout money? Okay, sell everything you've got, including that low-risk Vanguard Utilities Fund ( VPU). Greece is back on board? Very good, then buy Small Cap Australia ( KROO) to profit from the


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It only takes a minute to get started. Fill out our simple sign up form and login to the site.
This step is elective but will greatly multiply your chances of getting interest from other over 40s members on the site. Type a little about yourself, your pasttimes and attach a nice photograph of yourself.
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"Time After Time" is the twentieth episode of the third season of Grey's Anatomy and the 56th produced episode of the series. It is also the 56th produced episode of the Grey's Anatomy franchise. It was broadcast on April 19, 2007.
Colin Marlow continues to try and come inbetween Cristina and Burke and intrude on their lives, and his confrontation with Burke escalates when they treat a patient together. When the daughter she gave up for adoption years ago comes to the hospital suffering from leukemia, Izzie finds her past coming back to haunt her and finds her daughters lives in her hands when she needs a bone marrow transplant and turns to George for help. Alex believes he's found Ava's real family and Derek's relationship with Meredith interferes with his chances of becoming the next Cheif.
- "A patients history is as import as their symptoms. It's what helps us decide if heartburn is a heart attack, if a headache is a tumour. Sometimes patients will try to rewrite their own histories. They'll claim they don't smoke or forget to mention certain drugs which in surgery can be the kiss of death. We can ignore it all we want, but our history eventually always comes back to haunt us."
- "Some people believe that without history our lives amount to nothing. At some point we all have to choose, do we fall back on what we know or do we step forward to something new? It's hard not to be haunted by our past. Our history is what shapes us, what guides us. Our history resurfaces time after time after time. So we have to remember, sometimes the most important history is the history we're making today."
- "You Know I'm No Good" - Amy Winehouse
- "Burning" - The Whitest Boy Alive
- "Dancing With Myself" - Nouvelle Vague
- "Clean Getaway" - Maria Taylor
- "Fontana Fontaine" - Lullaby Baxter
- "A Good Start" - Maria Taylor
Title ReferenceEdit
This episodes' title references the song Time After Time, originally sung by Cindy Lauper


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Fetuin-A is a known inhibitor of vascular calcification in vitro. In arteries with calcification, there is increased immunostaining for fetuin-A. However, vascular smooth muscle cells (VSMC) do not synthesize fetuin-A, suggesting fetuin-A may be endocytosed to exert its inhibitory effects. To examine the mechanism by which fetuin-A is taken up in bovine VSMC (BVSMC), we examined living cells by confocal microscopy and determined the uptake of Cy5-labeled fetuin-A. The results demonstrated that fetuin-A was taken up in BVSMC only in the presence of extracellular calcium, whereas phosphorus had no effect. Additional studies demonstrated the calcium-dependent uptake was specific for fetuin-A and only observed in BVSMC and osteoblasts, but not epithelial, endothelial, or adipose cells. The uptake was dose dependent, but could not be inhibited by excess unlabeled fetuin-A, suggesting a fluid phase rather than a receptor-mediated process. Fetuin-A also induced a sustained increase in intracellular calcium in BVSMC in the presence of extracellular calcium, whereas there was no increase in the absence of extracellular calcium. To further characterize the uptake, we utilized an inhibitor of annexin calcium channel activity, demonstrating inhibition of both fetuin-A uptake and intracellular calcium increase. Finally, we demonstrate that fetuin-A binds to annexin II at the cell membrane of BVSMC. In summary, our study demonstrates calcium- and annexin-dependent uptake of fetuin-A that leads to a sustained rise in intracellular calcium. This regulated uptake may be a mechanism by which fetuin-A inhibits VSMC calcification in the presence of excess calcium.
- annexins
- cellular uptake
- intracellular calcium
- vascular calcification
vascular calcification is common in aging, diabetes, and kidney disease and is associated with increased cardiovascular morbidity and mortality (18, 19, 23). Risk factors vary in these patient groups, but advanced age and inflammation are commonly associated with increased vascular calcification. Recent attention has focused on fetuin-A, a reverse acute-phase protein, with levels inversely related to C-reactive protein and proinflammatory cytokine serum levels. Fetuin-A (α-2-Heremens Schmidt glycoprotein) is an abundant serum glycoprotein of 65,000 Da that is a member of the cystatin superfamily of proteins (25). Ketteler et al. (13) found that low levels of fetuin-A are associated with cardiovascular mortality in dialysis patients, data confirmed by two other groups (26, 30). Low fetuin-A levels in dialysis patients are associated with increased coronary artery calcification by spiral computed tomography scan (20), valvular calcification (30), and increased intima medial thickening of the carotid arteries (26). Fetuin-A knockout animals have diffuse extraosseous calcification (24), indicating an important role for fetuin-A in the inhibition of unwanted mineralization. Fetuin-A binds to calcium and phosphorus to create small particles in serum, accounting for 50% of the effect of serum to inhibit calcium-phosphate precipitation (10). These data support a potential role for fetuin-A in the regulation of extraskeletal calcification.
Fetuin-A also appears to have a direct cellular inhibitory effect on vascular calcification. Fetuin-A can decrease calcification of osteoblasts (10) and vascular smooth muscle cell (VSMC) in vitro (20, 22). We have also found that fetuin-A was present by immunostaining in areas of atherosclerosis and medial calcification in the inferior epigastric artery of chronic kidney disease patients (20). Since VSMC do not synthesize fetuin-A (28), this implies that the fetuin-A deposits there in an attempt to limit calcification. Reynolds et al. (22) recently demonstrated that, when fetuin-A was present in matrix vesicles from VSMC, they were rendered less capable of mineralization, suggesting that the cellular uptake of fetuin-A may have intracellular effects on the development of matrix vesicles to inhibit mineralization. However, the mechanism by which VSMC may take up fetuin-A is unknown.
A recent study in a breast carcinoma cell line demonstrated that membrane expressed annexin II and VI bind to immobilized fetuin-A in a calcium-dependent manner (17). Annexins are a family of closely related calcium and membrane binding proteins expressed in most eukaryotic cell types with diverse functions, including vesicle trafficking, cell division, apoptosis, calcium signaling, and growth regulation. In addition, several annexins, in particular annexins I, II, and VI, are present on endosomal compartments and are involved in endocytosis (6). In vitro, calcium entry into chondrocytes upregulates annexins II, V, and VI, which in turn mediate calcium influx into matrix vesicles (31, 32). Furthermore, mineralization of chondrocyte matrix vesicles is inhibited by antibodies to annexins II and VI (16). The purpose of the present study was to determine the mechanism of fetuin-A uptake by bovine VSMC (BVSMC) and the role of annexins in this cellular uptake.
Cell culture.
BVSMC were isolated from thoracic aorta by the explant method, as previously described (3). The BVSMC were grown in Dulbecco's modified Eagles medium (DMEM; Sigma, St. Louis, MO), with 10% FBS until confluent, at which time they were reseeded for specific experiments. In some experiments, BVSMC were exposed to serum-free media for 24 h before the experiments. Only cells between passages 2 and 8 are used in the experiments. These cells express α-smooth muscle actin, as well as basal alkaline phosphatase, and Runx2 (Cbfa-1) (2).
Fluorescent labeling of fetuin and live cell imaging.
To determine the mechanisms of BVSMC uptake of fetuin-A, we examined living cells using confocal microscopy. Fetuin-A from fetal calf serum (Sigma) was labeled with FluoroLink Cy5 mono-functional dye (Amersham Biosciences, Piscataway, NJ). It was separated on a column of Bio-Gel P-30 Gel (Bio-Rad Laboratories, Hercules, CA). Cy-5-labeled bovine albumin of similar size (66 kDa, Sigma, St. Louis, MO) was used as a protein control. Dextran-Texas Red of similar size (70 kDa; Molecular Probes, Eugene, OR) was also used as a control for the endocytic process. In some experiments, Cy-5-labeled asialofetuin-A was also used. To determine cellular specificity, we also examined uptake of fluorescently labeled fetuin-A in both mineralizing and nonmineralizing cells, including MC3T3 osteoblast cells, Madin-Darby canine kidney (MDCK) renal epithelial cells, human aortic endothelial cells, and 3T3-L1 adipocytes (gifts from Drs. R. Duncan, R. Bacallao, K. March, and R. Considine, Indiana University). BVSMC and these other cells were seeded on glass bottom microwell dishes (MatTek, Ashland, MA) in 10% FBS DMEM for 72 h. Ten minutes before addition of Cy5-fetuin, the media was replaced with M2 media (150 mM NaCl, 20 mM HEPES, 0 or 1.3 mM CaCl2, 5 mM KCl, 1 mM MgCl2, 50 mM glucose, at pH 7.4) at 37°C. Labeled fetuin-A was then added, and a MRC-1024 laser scanning confocal microscopy (Bio-Rad) was used to capture images. Plates were kept at a constant 37°C.
To determine the effect of calcium and phosphorus on the fetuin-A uptake, BVSMC were incubated with M2 media with 0, 1.3, and 5 mM CaCl2, with or without various concentrations of NaH2PO4. BVSMC were also pretreated with various doses of K201 (11), a specific annexin calcium channel blocker (kindly provided by Aetas Pharma) for 24 h before switching to test media. In some experiments, BVSMC were pretreated with 30 μM BAPTA-AM, an intracellular calcium chelator (Calbiochem), or 10 μM nifedipine (selective blocker of L-type Ca2+ channel, Calbiochem) for 30 min before live cell imaging of fetuin-A uptake experiment. Each experiment was repeated three to six times, with at least two dishes per experiment for a total n of 6–12. To quantify the uptake of fluorescently labeled fetuin-A, image processing was conducted using Metamorph software (Universal Imaging, West Chester, PA). Six images with four to six cells per image were quantified to allow for a representative assessment of the uptake.
Western blotting.
Western blotting was performed as previously described (3). The blots were incubated with rabbit antibodies against annexin II (1:1,000, Santa Cruz Biotechnology, Santa Cruz, CA) or bovine fetuin-A (1:2,000, a gift from Dr. Willi Jahnen-Dechent, Aachen, Germany) overnight at 4°C followed by incubating with peroxidase-conjugated secondary antibody (1:5,000 dilution). Immunodetection was with the Enhanced Chemiluminescence Kit (Amersham, Piscataway, NJ). The band intensity was analyzed by scanning densitometry (Quantity One, Bio-Rad, Richmond, CA).
Intracellular Ca2+ measurement.
To determine the effects of fetuin-A on cytosolic free Ca2+ concentration ([Ca2+]i), BVSMC were grown on a glass coverslip in DMEM with 10% FBS. The cells were then switched to serum-free media for 24 h to minimize residual effects of fetuin-A. Before the analysis for [Ca2+]i, cells were loaded with 3 μM fura 2-AM (Molecular Probes, Eugene, OR), a fluorescent [Ca2+]i chelator, in Hanks' balanced saline solution for 30 min at 37°C. Cells were rinsed and incubated for an additional 30 min with Hanks' balanced saline solution alone to allow for complete deesterification of the fluorescent probe. A ratiometric video-image analysis apparatus (Intracellular Imaging, Cincinnati, OH) was used to determine changes in [Ca2+]i. The ratio of consecutive frames obtained at 340 and 380 nm is determined, and the [Ca2+]i in each cell is calculated from this ratio by comparison to a fura 2 free acid standard curve, as previously described (4). The net [Ca2+]i response was calculated by determining the percent increase of peak calcium levels over baseline in response to the addition of fetuin-A in the presence or absence of 10 μM K201 in 0 or 1.3 mM CaCl2. Bovine serum albumin was used as a control for nonspecific findings. In some experiments, 10 μM ionomycin was used as positive control for [Ca2+]i measurement.
Plasma membrane protein preparation and immunoprecipitation.
BVSMC were incubated in serum-free media in the presence or absence of high calcium and phosphorus concentrations (2.6 mM CaCl2, 2.0 mM NaH2PO4 = high Ca × Pi), normal calcium and phosphorus concentrations (1.8 mM CaCl2, 0.9 mM NaH2PO4 = normal Ca × Pi), with or without fetuin-A (500 μg/ml) and cell membrane fractions of BVSMC isolated using the ProteoPrep Membrane Extraction Kit, according to kit instructions (Sigma Chemical). To analyze the interaction between annexins and fetuin-A, 500 μg of plasma membrane protein were immunoprecipitated with rabbit antibody against fetuin-A in the presence of ExactaCruz immunoprecipitation reagent (Santa Cruz Biotechnology). The pellet was washed with PBS and resuspended in 2 × reducing buffer, boiled, and loaded onto a 10% SDS-PAGE. Western blotting was then performed as described above with antibody against annexin II.
Statistical analysis.
The difference in quantified fetuin-A uptake by live cell imaging, bands assessed by densitometry on Western blot, or percent change in intracellular calcium in BVSMC in response to various treatments were compared by ANOVA with Fisher's post hoc analysis. The results are expressed as means ± SD, with P < 0.05 considered significant (StatView, SAS Institute, Cary, NC).
Fetuin-A is endocytosed by BVSMC in a calcium-dependent manner.
To confirm that fetuin-A was endocytosed, BVSMC were incubated with 0, 1.3 (normal), or 5 mM CaCl2 (high calcium) in the presence of Cy5-labeled fetuin-A (100 μg/ml) and examined using confocal microscopy, with the magnitude of fetuin-A uptake quantified by MetaMorph software. We determined that fetuin-A was endocytosed by BVSMC after 25–35 min, and that this was a calcium-dependent process (Fig. 1, A and B). As shown in Fig. 1C, there is minimal fetuin-A uptake in BVSMC when there is no extracellular calcium. Quantitative analysis showed a 4.7-fold increases in fetuin-A uptake in the presence of normal (1.3 mM) extracellular calcium compared with 0 mM calcium (P < 0.05). However, there is no difference in fetuin-A uptake between normal (1.3 mM) or high (5 mM) extracellular calcium concentrations (Fig. 1C). To confirm the live cell imaging uptake of fetuin-A, BVSMC were also incubated with or without fetuin-A (100 μg/ml) in the presence of various calcium concentrations after serum starvation for 24 h, and cell lysate was isolated for Western blot of fetuin-A (Fig. 1D). The results confirm the live cell imaging experiments, demonstrating cellular fetuin-A uptake is dependent on extracellular calcium. Since BVSMC were incubated with 10% FBS presumably containing relatively high amounts of fetuin-A, we performed the additional experiments in which BVSMC were exposed to serum-free media for 24 h before labeled fetuin-A was added for live cell imaging. The results demonstrated that, while the fetuin-A uptake occurred earlier (30 min after adding labeled fetuin-A) in BVSMC with overnight serum starvation compared with BVSMC without serum starvation (40 min after adding labeled fetuin-A), the uptake reached the same degree by 60 min (data not shown). Furthermore, exposure of BVSMC to serum-free media for 24 h did not change the calcium-dependent pattern of fetuin uptake.
To characterize the role of phosphorus in fetuin-A uptake, BVSMC were incubated with increasing concentrations (0, 1.8, or 5 mM) of NaH2PO4 in the presence or absence of normal (1.3 mM) calcium, and fetuin-A uptake was examined by confocal microscopy and quantified. The results demonstrated that, in the absence of extracellular calcium, phosphorus alone had no effect on fetuin-A uptake in BVSMC (Fig. 2A). Furthermore, in the presence of 1.3 mM extracellular calcium, there is no difference in fetuin-A uptake with increasing concentrations of phosphorus (Fig. 2B). These data indicate that phosphorus had no additive effect on the calcium-dependent fetuin-A uptake in BVSMC (Fig. 2B).
To further evaluate the calcium-dependent uptake of fetuin-A in BVSMC, we performed additional experiments. First, to determine whether the calcium dependency of fetuin-A uptake is specific for fetuin-A, uptake of Cy-5-labeled bovine albumin and Texas-red-labeled dextran of similar molecular weight as fetuin-A were examined in the absence or presence of extracellular calcium concentration. The results demonstrated that fluorescently labeled fetuin-A dextran and albumin were all taken up by the BVSMC, but only the uptake of fetuin-A was calcium dependent. The albumin and dextran uptake in BVSMC was independent of extracellular calcium (data not shown). Second, to determine the cell specificity of fetuin-A uptake, we examined the uptake of Cy5-fetuin-A, with and without 1.3 mM calcium, in MDCK renal epithelial cells, human aortic endothelial cells, 3T3-L1 adipocytes, and MC3T3 osteoblasts. The results demonstrated that a similar calcium-dependent uptake was observed only in MC3T3 osteoblasts. Fetuin-A was also taken up, but in a non-calcium-dependent manner, in MDCK cells, endothelial cells, and adipocytes (Fig. 3). These results suggest that calcium dependency of fetuin-A uptake is unique for BVSMC and MC3T3 osteoblasts. Interestingly, these are also the only two cell types that can also mineralize in vitro in the presence of calcium and phosphorus. To determine the mechanism by which BVSMC take up fetuin-A, cells were incubated with increasing doses of Cy5-labeled fetuin-A (25, 50, or 100 μg/ml), and uptake was examined by confocal microscopy and quantified. As demonstrated in Fig. 4A, there is a significant dose-dependent uptake of fetuin-A in BVSMC (P < 0.05). BVSMC were also incubated with Cy5-labeled fetuin-A in the presence or absence of 20-fold unlabeled fetuin-A and examined by confocal microscopy. The results demonstrated that excess unlabeled fetuin-A had no effect on Cy5-labeled fetuin-A uptake (Fig. 4B). The finding of a dose-dependent uptake, together with an inability of excess fetuin-A to block uptake, suggests the mechanism of endocytosis is fluid phase rather than receptor mediated. Interestingly, asialofetuin-A, like other asialo-glycoprotein, is bound by the asialo-glycoprotein receptor on the cell surface and internalized (29). Indeed, we have found that excess unlabeled (20-fold) asialofetuin-A can partially block Cy5-labeled asialofetuin-A uptake in BVSMC (data not shown), suggesting a receptor-mediated process for asialofetuin-A uptake.
Fetuin-A increases intracellular calcium in BVSMC.
To determine whether calcium-dependent fetuin-A uptake in BVSMC is associated with an alteration in intracellular calcium levels, we measured [Ca2+]i using fura 2 by ration fluorescence spectrometer. In the presence of normal extracellular calcium (1.3 mM CaCl2), the addition of fetuin-A (1 mg/ml) induced a sustained increase in [Ca2+]i in BVSMC (Fig. 5A). However, in the absence of extracellular calcium (0 mM CaCl2), there was no increase in intracellular calcium in BVSMC when fetuin-A was added (Fig. 5B). Addition of bovine serum albumin to the BVSMC had no effect on intracellular calcium levels (data not shown). To ensure the addition of fetuin-A with or without calcium did not injure the cells, 10 μM of the calcium ionophore ionomycin was added after the fetuin-A. As shown in Fig. 5, there was still a rapid and appropriate increase in [Ca2+]i in BVSMC, indicating that the fetuin-A did not alter normal intracellular calcium responsiveness.
Blocking annexin calcium channel activity prevents fetuin-A uptake and rise in intracellular calcium in BVSMC.
To determine the role of annexins in the calcium-dependent uptake of fetuin-A in BVSMC, cells were pretreated with increasing concentrations of K201, an inhibitor of annexin Ca2+ channel activity (11, 14) for 24 h, and fetuin-A uptake was examined by confocal microscopy and quantified. The results demonstrate that K201 dose-dependently inhibited calcium-dependent fetuin-A uptake in BVSMC (Fig. 6A; 34% inhibition in fetuin-A uptake with 5 μM K201; 70% inhibition in fetuin-A uptake with 10 μM K201; *P < 0.05 compared with 0 μM K201, #P < 0.05 compared with 5 μM K201, n = 6). However, treatment of BVSMC with BAPTA-AM, an intracellular calcium chealator, or nifedipine, a selective blocker of L-type Ca2+ channel, had no effect on fetuin-A uptake in BVSMC (data not shown). These data suggest that annexins mediate the calcium-dependent uptake of fetuin-A in BVSMC.
To determine the role of annexins in fetuin-A-induced changes in intracellular calcium, BVSMC were pretreated with K201 for 24 h, and intracellular calcium was examined. As shown in Fig. 6B, while fetuin-A increased [Ca2+]i by 25 ± 9.5%, K201 treatment completely blocked fetuin-A-induced increase in intracellular calcium in BVSMC (0.8 ± 0.4%, P < 0.05). However, K201 treatment had no significant effect on ionomycin-induced increase in [Ca2+]i (43 ± 10% increase for control cells treated with ionomycin alone; 49 ± 11% increase for K201 pretreated cells with ionomycin). These results support that, in BVSMC, annexins mediate the calcium-dependent uptake of fetuin-A and the sustained rise in [Ca2+]i in response to fetuin-A.
Fetuin-A binds to annexin II in BVSMC.
To characterize the interaction of fetuin-A and annexins further, we performed two sets of experiments. First, to determine whether fetuin-A increased the membrane location of annexin, BVSMC were treated with normal Ca × Pi (1.3 mM Ca/1.4 mM Pi) or high Ca × Pi (2.6 mM Ca/2.0 mM Pi) in the presence or absence of fetuin-A (500 μg/ml), and membrane fractions were isolated. Western blot analysis of membrane proteins was performed to examine the existence of annexin II in the cell membrane. As shown in Fig. 7A, annexin II is present in the membrane of BVSMC, but neither high Ca × Pi concentrations or fetuin-A changed the quantity of annexin II in membrane fraction of BVSMC. Second, to determine whether there is binding of fetuin-A to annexin II at the cell membrane, BVSMC were incubated with high Ca × Pi or normal Ca × Pi to see if conditions conducive to mineralization changed their binding. Membrane fractions were isolated and then immunoprecipitated with antibody against fetuin-A to precipitate membrane fractions that contained fetuin-A. These fetuin-A containing fractions were then analyzed to determine whether they also contained annexin II Western blot. The results showed that fetuin-A-containing membrane fractions from BVSMC treated with high Ca × Pi had significantly more annexin II, whereas the membrane fraction of BVSMC that was treated with normal calcium phosphate concentration had little annexin II signal (Fig. 7B). These data suggest that, in cultured BVSMC, high-calcium phosphate enhanced annexin II binding to fetuin-A at the cell membrane.
Fetuin-A is known to bind to calcium and phosphate in serum to decrease the supersaturation of serum and thereby reduce extraskeletal calcification. In addition, we and others have identified fetuin-A in arteries from chronic kidney disease and aging patients in areas of calcification (20, 21). As fetuin-A is not synthesized by VSMC, this implies that its deposition in areas of calcification may be a mechanism for local regulation of calcification. The present study demonstrates that VSMC can take up fetuin-A and that this uptake is dependent on calcium and is specific for fetuin-A (compared with dextran). Further supporting that this uptake may be a regulatory mechanism to control vascular calcification is our finding that the calcium-dependent cellular uptake of fetuin-A was also cell specific, only observed in VSMC and osteoblasts, the only cells tested that are capable of mineralizing. Fetuin-A constitutes a major component of the noncollagenous protein fractions of mineralized bone and plays an important role in bone formation and remodeling (1, 5). The fact that calcium-dependent fetuin-A uptake only occurs in BVSMC and osteoblasts, but not other types of cells, suggests that this pattern may be unique for mineralizing cells.
In addition to calcium, phosphorus is also a necessary and direct stimulus for calcification in BVSMC, with the effect of the two ions additive (21). However, we found no effect of phosphorus on fetuin-A uptake, consistent with work by Schinke et al. (25). They found 125I-labeled fetuin-A coprecipitated in mixtures of calcium and phosphate and in mixtures of calcium and carbonate, but not in mixtures of magnesium and phosphate, indicating that the binding of fetuin-A to hydroxyapatite is due to interactions with calcium ions rather than with phosphate ions (25). Once endocytosed, the fetuin-A uptake led to sustained increases in intracellular calcium, indicating a secondary cell signaling process as a result of the uptake.
The mechanism of the calcium-dependent uptake of fetuin-A in our live cell imaging was fluid-phase as opposed to a receptor-mediated process, as the uptake was not saturable with unlabeled fetuin-A, yet dose dependent. We also demonstrated that the uptake was dependent on annexin activity, as we could block the cellular uptake with K201, a compound that has been shown to inhibit annexin calcium channel activity (11, 14). Furthermore, we found evidence for binding of fetuin-A and annexin II on cell membranes of BVSMC, and increased calcium and phosphorus significantly increase this interaction of fetuin-A and annexin II. The latter finding is consistent with a recent report of calcium-dependent binding of both annexin II and annexin VI to immobilized fetuin-A in a breast cancer cell line (17). These authors also found knockdown of annexin II or VI with small-interference RNA significantly reduced fetuin-A binding to the breast cancer cell surface (17). Thus annexin may serve as a "receptor" or "gate" for fetuin-A, but without saturable binding. It is more likely that fetuin-A or calcium or both alter annexins, leading to conformational changes at the cell membrane. Support for this are a number of studies demonstrating that annexins are multifunctional cell surface receptors that interact with a variety of extracellular ligands, such as plasminogen activator (9), heparin (12), and chondroitin sulfate chains (27).
We also demonstrated that fetuin-A induced a sustained increase in [Ca2+]i in BVSMC. However, in the absence of extracellular calcium, there is a minimal increase in intracellular Ca2+ induced by fetuin-A. This rise in intracellular calcium was completely blocked by inhibition of annexin activity, whereas the intracellular calcium rise induced by ionomycin was not. These data indicate that the source of increased intracellular Ca2+ is from extracellular calcium. We hypothesize that fetuin-A, when it arrives at the membrane annexin "gate" with calcium, changes annexin conformation to allow both calcium and fetuin-A to enter the cells. If either fetuin-A is not carrying calcium, or annexins are blocked, then neither calcium nor fetuin-A enters the cells. This is plausible in that annexins are known to facilitate membrane conformational changes and the formation of lipid rafts that facilitate endocytosis in osteoblasts (7). Several studies have demonstrated that annexins are present on endosomal compartments and involved in endocytosis (6). For example, annexin II is found on early endosomes in complex with S100A10 to maintain the correct morphology of perinuclear recycling endosomes. Moreover, its depletion can interfere with the proper biogenesis of multivesicular endosomes from early endosomes (8). An alternative explanation is that annexins mediate calcium entry that then facilitates fetuin-A/annexin binding and fetuin-A endocytosis.
Our results strongly suggest annexin-mediated membrane organization and trafficking may be responsible for the fetuin-A uptake, which, in turn, may regulate the mineralization process in VSMC. Indeed, annexin has an important role in normal bone mineralization. Annexins II, V, and VI are highly expressed in hypertrophic and mineralizing growth plate cartilage, and the expression of annexins is indicative of terminal differentiation and chondrocyte mineralizing potential (15). In addition, annexins also form Ca2+ channels in matrix vesicles, enabling Ca2+ influx into these particles as an initial step for the formation of the first mineral phase in chondrocytes (14). Gillette and Nielsen-Preiss (7) overexpressed annexin II in osteoblasts, demonstrating a dramatic increase in alkaline phosphatase activity and mineralization. Our enhanced binding of annexin II and fetuin-A in BVSMC in conditions known to induce mineralization also support a role for annexin II in fetuin-A regulation of vascular calcification. Supporting this is recent data from Reynolds et al. (22), demonstrating that the presence of fetuin-A in matrix vesicles from VSMC renders them less able to mineralize. Clearly we are only beginning to understand this process, and the intracellular signaling pathways by which they may occur require further studies.
In conclusion, our study demonstrates that fetuin-A uptake by BVSMC is dependent on extracellular calcium and annexin activity, and that fetuin-A binds to annexin II at the cell membrane of BVSMC. Once endocytosed, fetuin-A induces a sustained rise in intracellular calcium, which may facilitate its regulatory role in VSMC calcification.
This work was supported by an unrestricted research grant from The Genzyme Corporation (N. X. Chen), K01 Grant from National Institute of Diabetes and Digestive and Kidney Diseases (N. X. Chen), and Veterans Affairs Merit Award (S. M. Moe).
S. M. Moe has a consultancy with, and has received honoraria from the Genzyme Corporation.
The authors thank Dr. Randall Duncan (University of Delaware) for providing technical advice regarding intracellular calcium imaging, and Michelle Murray for excellent secretarial assistance.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2007 the American Physiological Society


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Photos by Madeline Pado
Phoenix's public spaces were discussed on Wednesday evening by a panel of community activists as part of the last Downtown Devil Discussion of the semester.
The subject covered in the discussion did not stray far from the topics of public spaces, the characteristics that make them successful and what public spaces in Phoenix need.
The panel included Kirby Hoyt from Edge Industries, Jeff Moloznik from CityScape developer RED Development, Gangplank co-founder Derek Neighbors and Sean Sweat, an industrial engineer and transportation-planning expert.
"The ultimate public spaces are the streets," Sweat said. "In Phoenix, we think the streets are for cars, but that's wrong. The streets are for people."
Neighbors and Sweat both criticized ASU's decision to close its Downtown campus buildings to the public.
Sweat described the policy as "a violation of public space."
Hoyt focused on the problem of a sprawled out population in Phoenix, attributing downtown public spaces' lack of success to low population density.
The question of how to get more people to live in downtown neighborhoods and to inhabit these spaces was Hoyt's concern. "Without people, you've got crap," he said.
CityScape is helping downtown attract visitors who are used to staying home, Moloznik said.
"We do live in a historically backyard community that's realizing how fun it is to be in the front yard," he said.
The panelists also discussed the architecture of public spaces, especially the inward-facing design of CityScape. Sweat said CityScape has a suburban design that "shuns the space all around it."
Cities are not always eager to be in the business of operating public spaces due to potential problems and costs, Neighbors said, adding that the solution to this would be attracting people who want to be involved.
"Let social capital drive your operational model," Neighbors said. "The people that participate in the space will take care of it."
Talented individuals and cities that retain and attract those talented individuals are successful, he said, adding that part of the problem with Phoenix is its relative newness as a city.
"We're still finding our identity as a city. The next five or 10 years will really be formative," Neighbors said.
Audience member Michael J. Vivien, an architect, recently moved to Phoenix from California and thought the discussion touched upon some good points, but he was most interested in the question of how to bring people downtown.
"Public interaction is a lacking feature of Phoenix," Vivien said.
Contact the reporter at email@example.com


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Need Appliance Repair Help? Ask a Technician for Answers ASAP
What do you mean they wont take it bacvk after 90 days? You should have a 1 yr warrenty on that.
Possible solution
,, Recovery tank full/automatic
shut off engaged
Remove and empty recovery tank
,, Blockage In tool on end of hose
,, Recovery tank lid not on
Make sure lid is on securely
replaced after stair or upholstery
If this answers your question, please click the ACCEPT BUTTON This is how I get paid for my skills & knowledge.Need more info ask as long as it pertains to the same question. BONUSES are appreciated. NOTE: As I am not there this is based on your observations and information that i get from you. Thank-you.


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Take A Tour Of British Columbia's Great Bear Rainforest
Take a ride on fishing boats, kayaks, floatplanes and helicopters.
Please respect the places you find on The Outbound.
Always practice Leave No Trace ethics on your adventures. Be aware of local regulations and don't damage these amazing places for the sake of a photograph.


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Minecraft is a game in which you use building blocks to construct the world around you. You begin the game on a desert island, then using blocks you can build sheds, houses, palaces – anything you can imagine. System Requirements CPU: Intel Pentium D or AMD Athlon 64 (K8) 2.6 GHz RAM: 4GB VGA: GeForce [ Okumaya Devam ]
This version is fully compatible with all previous 1.8 versions, but it is still highly recommend to update to 1.8.8 as soon as possible for both players and servers. Notable bugs fixed: Server may not start in some environments Potential server crashing exploit Client crashing exploit Lag exploit with banners Please make sure that you [ Okumaya Devam ]
System Requirements CPU: Intel Pentium D or AMD Athlon 64 (K8) 2.6 GHz RAM: 4GB VGA: GeForce 6xxx or ATI Radeon 9xxx and up with OpenGL 2 Support (Excluding Integrated Chipsets) OS: Windows XP or better, Mac OS 10.5.8 or better, Linux HDD: 150MB Note: Java (Java SE 6 Update 32 or Java SE 7 [ Okumaya Devam ]


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Wedding Memories That Last a Lifetime: The Ritz-Carlton, Dubai International Financial Centre Offers Advice to Brides-to-Be on Creating a Wedding Day to Remember
DUBAI, UNITED ARAB EMIRATES -- (Marketwired) -- Creating and organizing a wedding can be a daunting task for any bride. With this in mind, The Ritz-Carlton, Dubai International Financial Centre is providing brides-to-be of the luxury hotel in Dubai, with advice on turning their wedding day dreams into a reality. Each bride will be offered access to one of the hotel's wedding experts to carefully guide them through every stage of the preparations whilst ensuring no detail is overlooked, to provide a memorable day for the couple and their guests.
Creating a Wedding that Wows:
Tips from Maya Saliba, Weddings Concierge, The Ritz-Carlton, DIFC
1. Choose the perfect setting for the perfect day
Start by choosing the wedding venue as this will set the tone and theme for the rest of the wedding. For example, a wedding in a stylish city space such as the Samaya Ballroom at The Ritz-Carlton, DIFC, might better lend itself to an elegant, contemporary dress, whilst a flowing bohemian-style outfit may be more appropriate for an alfresco wedding day.
2. Ensure two stand-out moments to remember
Ensure a day to remember with two key stand-out moments that differentiate your wedding from the next. This could be as simple as reading a personalized poem, or serving a bespoke mocktail named in your honor; two talking points should guarantee guests remember your special day over the next.
3. Create a theme and bring it to life
Be it shabby chic or pink and pearls, choose a theme early on and pull it through all the key elements of your wedding; from the dresses and flowers, to the cake and menus, ensuring consistency in look and feel. Visit the Ritz-Carlton's 'I do' pin board on Pinterest for some inspiration: www.pinterest.com/ritzcarlton
4. Personal touches add personality
Don't be afraid to ask your venue for help with personal touches; they are there to help. For example, at The Ritz-Carlton, DIFC, we offer a service to all our brides called 'Crafted with Love'. Our Pastry Chef creates a miniature version of the wedding cake before the real thing to ensure every detail is as the couple envisaged, and any personal requests can be accommodated, however small and intricate.
5. Savor every second
When the day arrives, remember it's your day. Every hour or so, take a moment with your groom, or your mum or best friend at a ladies wedding, simply to take it all in, and ensure the memories stay with you well beyond the big day.
Weddings at The Ritz-Carlton, DIFC
The Ritz-Carlton, DIFC is able to accommodate both intimate celebrations and large scale weddings in Dubai, thanks to its expansive ballroom and stunning outdoor terraces. At the heart of the hotel is the impressive Samaya Ballroom, an opulent space that can host up to 900 banquet guests or 1,300 reception guests. Located on the ground floor, its separate driveway and entrance guarantee the utmost privacy and a heightened sense of occasion.
Alternatively, two alfresco terraces offer a lush urban oasis, perfect for smaller celebrations. The Terrace can accommodate up to 240 guests, whilst the magical Sunken Garden, surrounded by a serene landscape of palm trees and a dramatic water fountain, is the perfect option for a cocktail reception or intimate banquet.
For more information on weddings at The Ritz-Carlton, DIFC or to speak to a wedding expert, please call +971 4 372 2628. www.ritzcarlton.com/DubaiIFC
About The Ritz-Carlton Hotel Company, LLC
The Ritz-Carlton Hotel Company, L.L.C., of Chevy Chase, Md., currently operates 86 hotels in the Americas, Europe, Asia, the Middle East, Africa, and the Caribbean. More than 30 hotel and residential projects are under development around the globe. The Ritz-Carlton is the only service company to have twice earned the prestigious Malcolm Baldrige National Quality Award, an award that originated in 1987. Its purpose is to promote the awareness of quality excellence, recognize quality achievements of companies and publicize successful quality strategies. The Ritz-Carlton Hotel Company won the award in 1992 and 1999. For more information, or reservations visit www.ritzcarlton.com. The Ritz-Carlton Hotel Company, L.L.C. is a wholly-owned subsidiary of Marriott International, Inc. (NASDAQ: MAR).
Contact: Hannah George Email Contact Director of Public Relations The Ritz-Carlton, DIFC +971 (0) 55 300 4620


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Helen Williams BA, Dip P Couns, Cert Gestalt Therapy
Among the most nerve-racking parenting experiences is dropping one's baby off in daycare for the primary time, regardless of if the kid is 2-years-previous or six weeks previous (the minimal age many daycare facilities will accept a baby). It appears counterintuitive but I feel there's a kernel of truth on this that may help us with parenting younger youngsters too. Spank on their bottom and allow them to put books and clothes into their pants if they want as it not about pain, it's about getting their consideration and the sight of a paddle will try this for most children without the spank. The actions of our bodies, how we spend our time, and the way we serve these in our lives all provide evidence of the depth of our Love for God.
Self-discipline is difficult, however we must do it. If we love our children, we have to do these arduous things so their lives will likely be more balanced and full after they turn out to be adults. Cheekiemonkies Love: Each room comes with a bunk mattress that contains a single, double and pull-out mattress to accommodate two adults and two kids. Then again the web site for an actual world parenting resource might be very helpful. Certainly, no one can ever exchange the love and care which you'll solely get from your parents.
They love out of doors projects, gardening, building with units and hollow blocks, subject trips, and games. Though their parenting style was far more … Read More . . .


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It could be popular (aka "The Golden Three" -- Naruto / Bleach / One Piece) or it could be something a little less known but still great (Wolf's Rain comes to mind). I've seen many profiles on here saying they watch anime, so what's your favorite?
Mine: Steins;Gate. The increasingly intense pacing, character development, plot line, everything was great to me. I was a bit nervous about watching an anime about a microwave time machine at first (I immmediately got thoughts of Fooly Cool randomness) but I'm glad I watched it all the way through...
... and then rewatched it because I missed it when I finished it.
Most Helpful Girl
I am a huge Naruto/Shippuden/Akkipuden fan. I literally grew up with Squad 7 and the gang, so it's like they're my peeps.
I also love "Say I Love You", Digimon, the one with the wind boy and the fire princess. I think it's Kazuma No Stigma, and Mew Mew Power00|00|0
Kazuma no stigma was pretty good! Haven't seen it in awhile though. Thanks for answering!
- Show AllShow Less
Well, at least he had a good excuse. They just stopped Btoom! because of sales and completely left things on a cliffhanger with only 13 episodes :(
I kind of wish someone would just pick it back up, but at the same time, i think it may be too late.


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Survivors moving to Birmingham
The second series of post-apocalyptic BBC1 drama Survivors will be made in Birmingham, relocating from its previous base in Manchester.
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Request: Vibe and Open
This video is for the person that made me so wet and turned on that I came in less than five minutes. He asked that I use a vibrator on my clit while I held my pussy open so that he could see all the contractions. I get incredibly wet and all of the wetness is natural. I hope he, and you, enjoy. ;)


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Back To Previous Page
Yoga on Tap
- 1624 Wyoming Street Baton Rouge, LA 70802
- Tin Roof Brewing Co.
- March 15, 2017 - September 27, 2017
- Recurring weekly on Wednesday
- Time: 6:00 PM to 7:00 PM
- Price: Free
- (225) 337-7022
Unless our venue is privately rented out, we are partnered with LuLu Lemon to provide free, entertaining yoga classes every Wednesday. These classes happen inside our brewery or outside on our green space depending on the weather. We also offer happy hour specials on our brews immediately following the class.


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Researchers of the University of Tokyo and Nagoya Institute of Technology managed to move small objects with the help of a complex system of acoustic levitation: sound waves moved polystyrene particles with a diameter of 0.6 to 2 mm in three-dimensional space. Earlier moving items using the same system was possible in only two dimensions.
To move through the air drops of water, polystyrene particles, small pieces of wood and even screws, four rows of speakers were needed. These objects were moving in all directions within the limits permitted by the experimental conditions. Motion in this case was caused by ultrasonic standing waves.
The literature describes an experiment that can be made at home: holing a slip of paper over the ultrasonic generator so that its free end was 3-5 mm above the end of the rod, you need to press the generator, and the tip of the paper will go up and will hang over the rod under the influence of a sound wave.
The apparatus used in the experiment is much more complicated than the generator: sound waves with a frequency of above 20 kHz, which are not audible to the human ear, come from four sides and intersect inside the confined space. Thus, they form a movable focus, in which small objects hang in the air.
The direction of the waves can vary arbitrarily, while the object is moving. Sound levitation is a way to overcome gravity, scientists say. So even now acoustic levitation devices are used by organizations such as NASA.
Anna LeMind
Latest posts by Anna LeMind (see all)
- 6 Summertime Struggles Only a Socially Awkward Introvert Will Understand - August 14, 2017
- The Quiet Ones Can Easily Fall into These 4 Ego Traps – Is Your Ego Exploiting Your Introversion? - May 2, 2017
- Is There Life After Death? 5 Hints That Our Existence Doesn't End with the Physical Death - April 25, 2017
- 6 Reasons Why INTP Personality Type Is One of the Quirkiest and Most Misunderstood - February 17, 2017
- 5 Struggles of Being an Old Soul in a Young Body - January 16, 2017


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I have a for loop which is executed 10 times. I want to save the data in a separate .mat file for each time. For example: in the 1st loop, i want to save it as Results1.mat in the 2nd loop, i want to save it as Results2.mat .... in the 10th loop, i want to save it as Results10.mat
I want to write a loop in which the number of iteration or loops is stored in a variable. For example, if the loop is executed 5 times, my variable should have the entries as [ 1,2,3,4,5]
How to write a loop in matlab which is executed 10 times what is the difference between for and while loop?
pls give me a very short example program using the go to loop. thanksrn
pls give me a very short example of do while loop in c++rn
Explain the differences between for and while loop in Java.
How to write a while loop?
What is a for loop? How to implement them?
How to write a do while loop?
Functionalities of c++ Usage of loops:The functionality of loop is simply to repeat statement while the condition set in expression is true. Title: How to write a while loop? Description:Loops have a purpose to repeat a statement a certain number of times or while a condition is fulfilled. Syntax of while is: While(condition) { Statement; } For a loop the three main expressions are: initialization, condition and increment/decrement operation. In while lop, initialization is done outside the loop and the increment/decrement operation is done inside the loop. The while loop is repeated till the condition becomes false. Example: //tofindsum of n numbers Void main() { Int n,sum=0; Cout>n; While(n>0) { Sum=sum+1; n--; } Cout· Type is the data type of specifier of the data returned by the function. · Name is identifier. · Parameters are nothing but local variables within that function. · Statements is the block of statements called functions body which is surrounded by {} braces. Example: #include Int addition(int a,int b) { Int r; r=a+b; return(r); } Void main() { int z; z=addition(2,3); coutType is valid data type like int,char,string.. · Name is an identifier · Elements field is enclosed with square brackets [],specifies how many elements are present in the Array Example: #include Int a[]={2,3,4} Int n,result=0; Int main() { For(n=0;n Type is the data type ofpointer · Variable name is the name of variable of the pointer. Example: #include Void main() Int a=5,b=6; Int *p; P=&a; *p=5; P=&b; *p=6; Cout


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"Lift failure causing patient accident"
About: Sussex Partnership NHS Foundation Trust / Working age mental health care (inpatient) Sussex Partnership NHS Foundation Trust Working age mental health care (inpatient) BN13 3EP
Posted by Anonymous
My Sister is currently in your Amberly Ward and has latter stages of Huntingtons Disease which makes her ability to walk difficult and her body moves involuntarily so she falls easily. Your lift from this ward has been out of action for 4 days now and we have been told the engineer came Saturday and it cannot be just fixed, no indication when this will be sorted at all, it is the only lift to two wards of patients which is disgusting. The first day it was out of action my Sister fell down the hard hospital stairs, no staff had made sure she was either not going to attempt the stairs or to help her. Since she has fallen I was told that we/her were supposed to ask staff to help her and when I approached one member she said my Sister "can" walk the stairs herself and I had insist with this member of staff that she "cant". The ward is set up for the communal area to be on a different floor, which is where you have the dining/shopping/smoking areas and I am sure it is not just my Sister on those upper floors that has limited mobility or worse and without a lift cannot access the services downstairs to this part of of the hospital. This lift is also a problem for the visitors of the residents of these wards, for example my Father has Parkinsons and is not supposed to use the stairs at home let alone the ones in this part of the hospital. So either my Sister or my Father has to take the risk with the stairs for him to visit, being that unaided or not it is a big risk for both and not at all suitable. Warning to others with family in this area of the hospital.
Response from Rachael Kenny, Head of Involvement, Sussex Partnership NHS Foundation Trust 3 years ago
Thank you for the time you have taken to give us your views. It is really important that we know where there are problems so that we can sort them out.
There is sometimes a time lag for technical reasons between the time a comment is made on public websites and me receiving notice of them. This has been the case here and so by this stage you will know that the Sussex Partnership Patient Advice and Liaison Service and Amberley ward manager have been talking with you and your family about the best way to resolve the problems.
The lift is now working and we are very sorry for the difficulties that it caused you.
kind regards
Rachael Kenny
Patient and Public Involvement Manager
Sussex Partnership NHS Foundation Trust
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ISC West Physical Access Identity Program for HID Global
HID Global
: 24 March, 2010
(New Product)
HID Global is attending ISC West where it will be displaying its products for the physical access control and secure identity systems
HID Global will showcase the latest advances and offerings in physical access, logical access, and secure issuance solutions at this week's ISC West International Security Conference and Exposition. In addition to demonstrating its broad portfolio of products and previewing new technologies, the company will also share its perspective for innovation at its strategy briefing.
"Innovation in the security industry is a state of mind, and can only be driven through a combination of actively listening to customers, strategically investing in technology, being a committed industry participant, and cultivating rock-solid partnerships and alliances," said Denis Hébert, HID Global's president and CEO. "Customers are the primary focus of our business, which is why we continue to expand our Genuine HID Secure Identity initiative. This initiative extends and enhances the HID Global customer experience through a unique set of value-added customer and channel partner advantages that we will be highlighting at ISC West."
HID Global will showcase its extensive suite of products, technologies, resources and partnerships for secure identity-related applications at Booth #12051 at the Sands Expo & Convention Centre, March 24-26. Key HID Global demonstrations at ISC West include:
* New Printing and Encoding Solutions: HID Global is previewing a new family of intuitive, modular, reliable and scalable FARGO Direct-to-Card printers, which offer solutions for creating customized photo IDs and encoding smart cards. Each FARGO model includes optional field-upgradable modules to scale to future requirements and works seamlessly with other HID products and technologies to optimize security and interoperability.
* Next-Generation Reader Technology: HID Global will be highlighting its next-generation, modular Edge Evo reader line for a variety of access-control applications. Also on display is the company's multiCLASS Magnetic Stripe reader line, designed for customers seeking to upgrade their current access control card system from the popular magnetic stripe technology to the enhanced security of 13.56 MHz smart card technology.
* Genuine HID Credential Solutions: Booth displays will highlight high-quality Genuine HID technology cards that enable users to seamlessly manage multiple applications and migration projects through a single credential containing diverse technologies.
* Genuine HID Technology: HID Global will be featuring more than 25 products from third-party developers who have embedded HID technology into products that extend customer value as part of the "Genuine HID Technology" ecosystem.
* HID Connect: Expanding the use of HID Global technology beyond HID-manufactured products, HID Connect, the hardware and software partnership arm of HID Global, promotes applications and products enabled by "Genuine HID Technology." HID Global booth visitors can view offerings from HID Connect Partners like Assa Abloy, Accu-Time, Axcess International, SMI and more.
* Networked Access and Advantage Partner Solutions: IP-enabled access control solutions based on HID Global's OPIN API development platform will be on display from development partners AMT, PCSC, PlaSec and Quintron. In addition, look for the more than 60 Advantage HID Channel Partners on display at ISC West who form an essential link in the Genuine HID value chain.
* Experience Zones: HID Global will also be promoting its solutions in several "Experience Zones" that span vertical markets including Health Care, Commercial, Education and Government. For each vertical market, the company offers a comprehensive ecosystem of products, resources and partnerships that help to speed the deployment of HID Global solutions.
HID Global executives will be featured at several events throughout ISC West, including:
* HID Global's "Strategies for Innovation" lunch strategy briefing: At the company's ISC West strategy briefing, Denis Hèbert, president and CEO will present HID Global's innovative strategies that provide significant advances in secure-access and identity-management technology, products, solutions and programs. Five HID Global partners will also be sharing their perspectives in a special panel discussion. The briefing is Wednesday, March 24, from 12:00 p.m. to 1:00 p.m., and will be followed by an exclusive media and analyst Q&A-only session from 1:05 p.m. until 1:30 p.m., in the Murano Room (3205) of the Venetian/Palazzo Congress Center, Level 3.
* Brad Jarvis, HID Global's vice president of product marketing, will present "Trends in Secure Identity Solutions: Findings, Analysis and Recommendations," and investigate current and future industry requirements and key challenges facing the physical and logical access control market. The primary research was based on a survey deployed and compiled by Chris Corum, Editor and Publisher of Avisian and provides an independent secure identity market study to look at business trends and related technology innovations such as virtual credentialing and security beyond hardware. This track session presentation will be held at 10:15 a.m. on Wednesday, March 24.
* Denis Hébert, HID Global's president and CEO, will be a panelist in the Security Industry Association's closing ISC West panel session, entitled, "The Look Ahead: Security Technology in the Next Decade," at 4 p.m. on Thursday, March 25, 2010, in the Venetian, Room 502.
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July 7, 2009
Award: Honored by Alstom
Citing a long-standing and successful business relationship that includes close collaboration on technology, French power generation and rail transport group Alstom has honored EPCOS as one of its preferred suppliers. Alstom is a key player in the construction of power plants and a leading provider of rail transport systems, including France's high-speed train TGV. Railcars from Alstom are deployed in intercity and local rail networks around the globe. EPCOS supplies Alstom primarily with power capacitors and screw-on aluminum electrolytic capacitors for use in rail systems.
A global presence – 24 production and development sites close to the customer worldwide – was a crucial factor in helping EPCOS win this award from Alstom. EPCOS manufactures a very large number of products for rail systems in countries where rail transport is expanding on a huge scale such as in China and India.
Further information on these products can be found at www.epcos.com/alu_screw and www.epcos.com/power_capacitors
Please forward reader inquiries to:
Fax +49 89 636-22471


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2015 BMW X3 XDrive28d Rated at 30 MPG Combined
The Environmental Protection Agency (EPA) has come up with an official fuel economy rating for the 2015 BMW X3 XDrive 28d which offers significant gains over its gas-powered counterpart.
Its rated at 27 mpg in the city, 34 mpg on the highway for a combined 30 mpg, a full 6 mpg combined better than the X3 XDrive28i. When it comes to the competition, the BMW beats its closest competitor, the Mercedes GLK250 Bluetec diesel, by 2 mpg combined.
SEE ALSO: 2015 BMW X3 Pricing
A base 2015 BMW X3 will sell for $39,325, while the diesel model commands a $42,825 price tag. For that price bump, you will get 6 more miles per gallon, along with 20 extra lb-ft of torque compared to the gas-powered model, as the 2.0-liter diesel makes 180 hp and 280 lb-ft of torque.
Discuss this story at our BMW Forum


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It's time to Dress up, dress all-out, and undress with New York's greatest burlesque show. This year Wasabassco celebrates Halloween on Saturday Oct 29. Avoid the maddening crowd-filled the streets and chaos and do halloween with class, elegance and lots of fancy stripping burlesquers.
It's a Halloween party Wasabassco style, with ridiculously costumed numbers from the performers, a costume contest for all of the guests, music, food and wine! Win prizes for you daring flair, including bottles of wine from City Winery, and enjoy one of the most acclaimed burlesque shows in the world.
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Doors at 7:00. Costume contest at 7:30. Showtime 8:00.
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I believe that the creative and design process that we develop as architects day by day goes beyond building edifices. On the contrary, I believe that the process should be more focused on providing benefits for society with our projects, spaces and forms alike that carry a social task that can make our society socially conscious and aware.
Architecture is not solely based on selling images and designs as a product, it surpasses that conception.For me, architecture is an understanding of geometry and composition of context to be able to regenerate it with a logical system that has the capacity to replicate itself. Use of technology should be a tool we must take advantage of to be able to systemize and rationalize patterns and algorithms forming structures that answer to a necessity.
Rojkind Arquitectos, Mexico City, Mexico, Media and Communication Director
3 ARCH, Mexico, MX, Junior Architect
Universidad Iberoamericana, Campus Santa Fe, Mexico City, Mexico. , BArch, Architecture Degree
Manuel Garibay Award, 1st Place
Best Thesis Project by Universidad Iberoamericana at 2015 / 2016
MCHAP Student Award, Nomination
Topographic Suture was nominated as best Student project by MCHAP 2017


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Ivy League Release
PHILADELPHIA - Penn men's soccer goalkeeper Max Kurtzman was named Ivy League Rookie of the Week on Monday after making eight saves over two matches for the Quakers in the past week of play.
Kurtzman made five saves in a 1-1 draw at Lehigh on Tuesday, with two saves coming in a busy second half as the Mountain Hawks made their comeback and his fifth save coming in the first overtime to preserve the tie game.
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Overall, Kurtzman has played every one of Penn's 1,208 minutes this season and is 6-5-2 in his rookie campaign. He has played the most minutes in goal of any Ivy League keeper and ranked fourth in goals-against average at 1.12. His 39 saves are third-most in the Ivy League and his save percentage of .722 is fifth.
The Quakers, sixth in the Ivy League with a 1-2-0 Ancient Eight mark, have a crucial Ivy tilt on the horizon when Yale comes to Rhodes Field on Saturday at 7:30 p.m. The Bulldogs are one of four teams right ahead of Penn in a four-team tie for second with six points. The match will be streamed live on the Penn Sports Network at PennAthletics.com following the women's match with Yale at 5 p.m.


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|Hiramori family
|Patron family:
|Daidoji family
|Crane Clan
|6th century
The Hiramori family were vassals to the Daidoji family, formed after the end of the Foxfire War. [1]
Origins Edit
For two generations the Daidoji Daimyo Daidoji Kamei and his children fought against his younger brother Daidoji Hira and his children. Hira and his followers fought against the Daidoji in the Uebe Marshes, using guerrilla tactics to outmanouvered their enemies for years. Hira's line was consumed, and two daimyo had been died during the war. Daidoji Kasako, Kamei's daughter, was the current daimyo. She was a practical woman who realized the value of such training despite the tragedy and gathered the troops that had helped her defeat Hira's followers, proclaimed as the Hiramori vassal family. The family was named the "Forest Hira," an ironic joke at the traitorous Hira's expense. Their ancestral home were the places where the Foxfire War was fought, the Akagi Forest, Uebe marshes, and southern hills. [2]
Duties Edit
The Hiramori family developed trap-laying and ambush strategies, keeping secret methods adapted to the most difficult ground in case anyone cared to follow their retreat. In war, the Hiramori were most valuable when Crane territory was deeply overrun. [3]
Bizarre Behaviour Edit
Hiramori bushi were reclusive, and cultivated repulsive quirks. They spoke in a strange backwoods accent and used to disappear without warning. The Hiramori hand-to-hand combat technique rejected armor and specialized in various lengths of improvised stakes and spears. Many Hiramori trained at the Harrier school, and a few of the more deadly Harriers ventured forth to learn the ways of the Hiramori. [3] There was always a high-ranking Hiramori in the Kosaten Shiro Dojo to serve as a sensei. His lessons were found among the more difficult, and the most popular, of the school. [4]
History Edit
The Hiramori operated against the Lion from the Akagi woodlands before and during the Clan War. When Yoritomo retreated through Crane lands during the War Against the Shadow, Hiramori Kedamono's band attacked his rear lines from the marshes. [3]
Hiramori daimyo Edit
|Hiramori Kedamono
|? - 1133
See also Edit
|This Crane Clan related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


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The Truth Is Sometimes Sobering, But Necessary.
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|Comments (1)
mycareer in San Diego, California
106 months ago
I just attended a job fair in San Diego, CA and the recruiter told us the Sobering Truth about the Pharmaceutical Company she's representing. I'm so grateful and enlighten thanks to her.
She said that the Pharmaceuticals Company in the next room is looking for the following:
For every job, there are over 1,600 applicants, including laid-off experienced pharmaceuticals professionals. Furthermore, they can afford to be picky and set almost inhumanly high requirements or barriers are what I like to call them.
If you changed jobs more the twice within a 5 year period, tough. They don't care about your circumstances, remember there are 1,600 applicants for every job that they can choose from.
She also said that a lot of us have a romantic view of what a pharmaceutical representative does, only to find out its not what we thought it would be.
There are a few more, but I think you get it.
My point is there is HOPE.
Keep your credit card in your wallet and don't be suckered by companies and organization claiming they can get your foot in the door. I've been told that most Pharmaceuticals companies will train you on their drugs. Also, talk to a few pharmaceuticals reps and see if they are willing to let you spend the day with them. If you still want to move forward, get the B2B experience and network with hiring managers of smaller pharma companies.
Much Success.
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