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De novo assembly of
In this study we conducted a high throughput RNA-seq approach to sequence the root transcriptome of
The resources generated in this study provide new insights into the upstream pathways to the synthesis of prostratin and will likely facilitate functional studies aiming to produce larger quantities of this compound for HIV research and/or treatment of patients.
Prostratin was first discovered during ethno botanical studies carried out on the island of Savai'i, Samoa, where native healers used the bark of
Very little is known about the biosynthetic pathway of prostratin, although a lot is known about its biochemical structure. The main pathways which are likely to play a role in the synthesis of prostratin and which were chosen for this study were the Terpenoid Backbone Biosynthesis (TBB) pathway (KEGG map00900) and the Diterpenoid Biosynthesis (DB) pathway (KEGG map00904). The TBB pathway is an important pathway for the synthesis of terpenoid or isoprene (5-carbon) compounds that are the building blocks for many important complex compounds synthesised further down the pathway (down stream) in other important biosynthetic pathways. The TBB pathway consists of two separate parallel biosynthetic pathways, one corresponding to the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway that takes place in the plastids, and another comprising the mevalonic acid (MVA) pathway that occurs in the cytosol. Both pathways lead to the synthesis of terpenoid building blocks: isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). These terpenoid compounds are then used to generate compounds geranyl diphosphate (GPP), farsenyl diphosphate (FPP) and geranylgeranyl diphosphate (GGPP), which are all precursor compounds to pathways further down stream, such as the DB pathway. The DB pathway is an important pathway, which begins with GGPP and leads to the synthesis of many important diterpenoids (20-carbon) compounds. The synthesis of one particular diterpenoid compound that is investigated in this study is casbene, which has a very similar skeleton structure to prostratin. It is highly likely that casbene is a precursor to prostratin
Similar properties to prostratin have been reported for 12-deoxyphorbol 13-phenylacetate (DPP), which has been derived from
Cross talk and/or interaction between distinct metabolic pathways are important to determine the potential metabolite profile of a cell under specific conditions. Distinct pathways may share common intermediate compounds for their downstream processing. An associated pathway to the DB pathway is the Kaurenol and the Zeatin Biosynthesis (ZB) pathway (KEGG map00908). Kaurenol is derived from GGPP and serves as a precursor for the synthesis of various gibberellin compounds that act as plant hormones modulating growth and development
In this study we present the results of next generation sequencing, de novo assembly and annotation of the
Results and Discussion
Sequencing and de novo transcriptome assembly
Next generation sequencing technologies have significantly facilitated a wide range of genomics applications including high throughput sequencing of non-model plant transcriptomes. To obtain
Table 1
Summary of
Assembly statistics
Total number of mate-pair reads (before trimming)
Total number of read base pairs (bp)
Average read length (before trimming; bp)
Total number of read mate-pairs (after trimming)
Total number of read singletons (after trimming)
Average read length (after trimming; bp)
Total number of ESTs
Total number of EST base pairs (bp)
Average EST length
677 bp
Total number of transcripts assembled (pre-isoform filtering)
Total number of transcripts assembled (post-isoform filtering)
Average length of all transcripts (bp)
Transcripts with E-value > = 1e-05 against nr
15,191 (83.6%)
Average length of transcripts (bp)
Additional file 1
Assessment of reads using FastQC before trimming. A) Quality of reads per base. The central red line is the median base quality, the yellow box represents the inter-quartile range (25-75%), the upper and lower whiskers represent the 10% and 90% points and the blue line represents the mean base quality. B) Distribution of mean quality scores over all sequenced reads. C) The GC content distribution over all sequenced reads as compared against the theoretical GC distribution. The blip in GC content above the theoretical GC distribution is most likely due to the utilized primers at the 5' end of the reads used during RNA-seq library preparation and sequencing.
Click here for file
Additional file 2
Assessment of reads using FastQC after trimming. A) Quality of reads per base after adaptive window trimming using a quality average threshold of 20 and a minimum length threshold of 20, The central red line is the median value, the yellow box represents the inter-quartile range (25-75%), the upper and lower whiskers represent the 10% and 90% points and the blue line represents the mean base quality B) The mean sequence quality scores over all reads. C) The GC content distribution over all sequenced reads as compared against the theoretical GC distribution.
Click here for file
To determine the best parameters for transcriptome de novo assembly using Oases
Additional file 3
Comparison of Oases assemblies using various k-mers. A) Oases assemblies using k-mers ranging from 17 to 47 with minimum transcript size of 100 bp. B) Oases assemblies with minimum transcript size of 300 bp. C) Zoomed in view of Oases assemblies with minimum transcript size of 300 bp over k-mers 19 to 37. The maximum N50, largest number of gene clusters and largest number of transcripts can be obtained using a k-mer of 25. Thus, this k-mer and a length threshold of > = 300 bp were selected as parameters to assemble the reference
Click here for file
Additional file 4
Evaluation of k-mer coverage for Velvet assembly. The frequency of k-mers (number of appearances or coverage) was determined using untrimmed Illumina reads and ESTs. The results show a large peak of k-mers with coverage of one, which mostly correspond to sequencing errors. Thus, a k-mer coverage threshold of 2 was utilized in the de novo transcriptome assembly.
Click here for file
Another feature of Oases is that attempts to cluster assembled transcripts into 'gene clusters' containing two or more putative alternative splicing (AS) isoforms. Our preliminary manual inspection of randomly selected gene clusters revealed that the majority of the predicted AS isoforms corresponded to spurious calls including RNA degradation products, sequence gaps denoted by Ns that were introduced in the scaffolding step and clustering of unrelated sense-antisense transcripts among others (Additional files
Additional file 5
Example of an Oases 'gene cluster'. A) Multiple sequence alignment of transcripts into the same 'gene cluster'. Note that transcript 2 (T2) is a 5'end truncation version of T6 and that T4 has a significant sequence variation. B) Blast homology screening revealed that T1, T3 and T5 are mitochondria encoded acetyl-CoA acetyltransferase transcripts.
Click here for file
Additional file 6
Clustering of GGPS with unrelated hypothetical proteins. A) Blast results of transcripts clustered into EFI_010585 isoform cluster using Oases. Note that only transcript 4 has similarity to GGPS and this is encoded in the reverse strand. B) Multiple sequence alignment of the reverse complemented GGPS transcript and a sequence representing the hypothetical transcripts.
Click here for file
Transcriptome annotation
To determine protein-coding transcripts we screened the
Table 2
Statistics of functional annotation of transcripts
Annotated Proteins
# transcripts
% Total transcripts
a) Protein-coding genes
Similar to known proteins
Conserved hypothetical proteins
Hypothetical proteins (ORF > = 80aa)
b) Non-coding RNA genes
Putative long ncRNAs
tRNA genes
5 (12)a,b
Pseudo tRNA genes
rRNA genes
2,171 (20)a
Total number of assembled transcripts
18,180 (18,187)a
aTotal number of tRNAs including four tRNAs identified in a transcriptome assembly using a shorter k-mer size of 17 and a minimum transcript length of 100 bp.
btranscripts EFI_002280 and EFI_003197 encodes three and two tRNA genes, respectively.
Additional file 7
Predicted tRNA genes in the
Click here for file
Additional file 8
Predicted rRNA genes the
Click here for file
Figure 1
The effect of query sequence length on the distribution of significant matches against NCBI non-redundant (nr) peptide database
The effect of query sequence length on the distribution of significant matches against NCBI non-redundant (nr) peptide database. The number of transcripts with matches (cut-off E-value of 1e-05) in NCBI peptide database (nr) is greatest with the longer assembled sequences.
Figure 2
Statistics of homology search of transcripts against nr peptide database
Statistics of homology search of transcripts against nr peptide database. A) E-value distribution of the top BLASTx hits with a cut-off E-value of 1e-05. B) Similarity distribution of the top BLASTx hits with a cut-off E-value of 1e-05. C) Species distribution of the top BLASTx hits is shown as a percentage of the total homologous sequences with an E-value greater than or equal to 1e-05.
The species distribution showed that the majority of top matches were to
We then evaluated the relative expression levels of
Functional and pathway annotations
To assign functional information to transcripts Gene Ontology codes were annotated using Annot8r
Figure 3
Frequencies and mean expression levels of transcripts matching GO terms
Frequencies and mean expression levels of transcripts matching GO terms. The percentage of transcripts matching GO terms is show for each category as grey bars and the normalized mean expression levels of transcripts matching each of these GO terms are shown as black diamonds.
Among the 2,428 transcripts assigned to the response to stimulus category, 370 and 257 were assigned to 'defence response' and 'response to cadmium ion', respectively. Interestingly, 191 of the transcripts assigned to defence response were associated with defence against bacteria, which correlates with the known anti-microbial properties of root extracts from Euphorbia species, such as
Under Molecular Function GO the two most abundant categories were 'binding' and 'catalytic activity' accounting for 33.2% and 11.8% of the transcripts, respectively (Figure
Next we evaluated the expression levels for each GO code by averaging the coverage of all transcripts matching to each GO code (see Methods). We found that the GO Molecular Function codes 'Structural molecular activity' and 'Antioxidant activity' showed the highest overall expression levels with an average mean coverage of 291 and 136, respectively (Figure
Figure 4
Frequencies and mean expression levels of transcripts matching KEGG pathways
Frequencies and mean expression levels of transcripts matching KEGG pathways. The percentage of transcripts matching to KEGG pathways within each high level category are shown as grey bars, while the normalized mean expression levels of transcripts matching each KEGG pathways are indicated as black diamonds. B) Number of transcripts for each secondary metabolite pathways is shown.
We next determined the overall expression levels for each KEGG pathway as described above for GO codes. The pathways with the highest expression levels were 'Translation' and 'Signaling molecules and interaction' with mean coverage values of 327 and 148, respectively (Figure
Comparison of
To evaluate the conservation of the
Figure 5
Euphorbiaceae comparative transcriptome analysis
Euphorbiaceae comparative transcriptome analysis. The BLAST program tBLASTx was used in conjunction with OrthoMCL using a threshold E-value of 1e-20 to identify orthologous genes between
Candidate genes upstream of prostratin biosynthesis pathway
Prostratin is a phorbol ester from the tigliane diterpene series. Recently casbene a product of the DB pathway (KEGG map00904) has been shown to be structurally similar to prostratin
Figure 6
Mean expression levels within the Terpenoid Backbone, Diterpenoid and Zeatin Biosynthesis pathways
Mean expression levels within the Terpenoid Backbone, Diterpenoid and Zeatin Biosynthesis pathways. A) Normalized mean expression levels for enzymes within the Terpenoid Backbone Biosynthesis (TBB) pathways, namely, plastidic 2-
Transcripts matching to genes encoding enzymes involved in the TBB, DB and ZB pathways were found through BLASTx nr searches using an E-value threshold of 1e-05 (Figure
The DB pathway is of particular interest, as one of the downstream products is the phorbol ester prostratin. All diterpenes begin synthesis from GGPP, a 20-carbon isoprenoid diphosphate, derived primarily from the MEP pathway (Figure
Casbene is considered the most likely precursor to prostratin
The expression levels of enzymes in the TBB and DB pathways were compared to those of enzymes in the ZB pathway. We found that the TBB pathway showed an overall higher expression level as compared to other downstream pathways. Interestingly 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (HDS) and isopentenyl diphosphate/dimethylallyl diphosphate synthase (IDS) from the MEP pathway presented the highest expression levels (Figure
To validate the observed RNA-seq expression trends we selected 16 transcripts encoding 8 enzyme types and designed primers for real time RT-PCR (Table
Table 3
Real time PCR primers used for expression validations of selected enzymes.
5'-Forward Primer Sequence-3'
5'-Reverse Primer Sequence-3'
Additional file 9
Validation of RNA-seq expression trends using a real time RT-PCR approach. Reciprocal (1/Ct) real time PCR values were averaged for each enzyme mentioned in Table
Click here for file
There were no transcripts with sequence similarity to geranyl diphosphate synthase (GPPS) identified in this study (Figure
The de novo assembly of the
Plant material, RNA isolation and deep sequencing
Live plants of
Total RNA was isolated according to the method described by Chang et al.
The library for transcriptome sequencing was constructed with Illumina's kit following manufacturer's protocol. The mRNA was purified from 10 μg of total RNA using oligo (dT) magnetic beads. After purification, the mRNA was fragmented into small pieces using divalent cations under elevated temperatures. The RNA fragments were used for first strand cDNA synthesis with random primers. Second strand cDNA synthesis was done by using DNA polymerase I and RNaseH. The cDNA fragments then went through an end repair process and were ligated to adapters. The products were purified and enriched with PCR before sequencing on the Illumina GAII sequencing platform. Image deconvolution and quality value calculations were performed using the Illumina GA pipeline 1.3.
RNA isolation and EST sequencing
Frozen root samples stored at -80°C were sent to the Beijing Genome Institute (BGI) at Beijing on dry ice. Total RNA was isolated as described above. The RNA was stored in a -80°C freezer until further processing.
Approximately 1 μg of total RNA was used for preparing a cDNA library using the Creator Smart cDNA Library construction kit (Clontech, Mountain View, CA, USA) following manufacture's instructions. The resulting second cDNA strand products were then run on an agarose gel and those with a size between 1-3 kbp were excised and purified using the QIAquick PCR Purification kit (QIAGEN, Shanghai, China) according to the manufacturer's protocol. The products were transformed into DH10B competent cells. Library was checked with a titer of 2 × 105 pfu/mL and a capacity of 1.2 × 106 clones. A total of 2,099 ESTs were sequenced using capillary sequencing. Vector sequences were removed and 1,884 good EST sequences with an average length of 677 bp and a minimum length of 101 bp were submitted to dbEST at GenBank. The assigned accession numbers are the following: [GenBank:
Transcriptome assembly
We evaluated several assemblers for the de novo assembly of the
The reads were first trimmed using the adaptive trimming function of a trimming perl script implemented by Nik Joshi at The Bioinformatics Core, UC Davis Genome Centre
In many instances Oases predicted spurious isoforms and to increase confidence in the assembled sequences a single transcript from each gene cluster was selected based on the following criteria: i) the transcript has the highest Oases confidence score (ratio of nodes in transcript to nodes in a gene cluster) that represents the transcript with the largest number of exons, ii) encodes the longest ORF, iii) corresponds to the longest nucleotide transcript, and iv) in cases where two or more transcripts have the same length then the one with highest sequence coverage is selected. This generated a dataset of 18,180 transcripts, of these 9,883 transcripts were submitted to GenBank/DDBJ/EMBL following their submission guidelines under the project ID 66759 and Locus Tag EFI. The remaining 8,297 sequences contained gaps denoted by 'Ns' that were introduced during the scaffolding step using pair-end short reads with an expected insert size of 200 bp. A fasta file of all 18,180 transcripts is presented in the Additional file
Additional file 10
Fasta sequences of 18,180 transcripts.
Click here for file
Transcriptome annotation
Protein-coding genes
Annotation by peptide sequence was done by searching transcripts against the NCBI non-redundant (nr) peptide database which includes all non-redundant GenBank CDS translations, RefSeq Proteins, PDB, SwissProt, PIR and PRF, excluding environmental samples from WGS projects. The search was conducted using BLASTx with an E-value cut-off of 1e-05 and matching to the top hits.
RNA genes
The assembled transcripts were scanned for the presence of tRNA and rRNA sequences using the programs tRNAscan-SE
Gene Ontology (GO) and KEGG pathways
The GO and KEGG annotations were carried out using the annotation program Annot8r
Related species comparison analysis
The EST datasets of closely related species, namely
Expression analysis and prostratin candidate genes
To determine the relative expression levels of
An in-house database of prostratin pathway related candidate genes was created by interrogating the literature and KEGG pathways for genes matching to the TBB, DB and the comparative downstream pathway, the ZB pathway. We then screened
RNA isolation, reverse transcription and Real-time PCR
Several enzymes from the Terpenoid Biosynthesis pathway (Figure
HIV: Human Immunodeficiency Virus; DPP: 12-deoxyphorbol 13-phenylacetate; EST: Expressed Sequence Tag; GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; ORF: Open Reading Frame; BWA: Burrows-Wheeler Aligner; AS: alternative splicing; AACT: acetoacetyl-coenzyme A (CoA) thiloase; CMS: 2-
Authors' contributions
DQ, RB and BC contributed with the conception and design of the research. YY and NZ performed real-time RT-PCR experiment. QT and YY collected plant material and isolated RNA samples for deep sequencing. BC carried out all bioinformatics analysis. PM and GK assisted the bioinformatics analysis. RB and BC conducted data analysis and wrote the manuscript. DQ and MB provided critical review of the manuscript and interpretations. All authors read and approved the final manuscript.
We acknowledge the National Collaborative Research Infrastructure (NCRIS) of Australia for providing access to bioinformatics computational pipelines used in this study and The Research Institute of Forestry, Chinese Academy of Forestry for financial support (ZD200902).
Diterpenoids from the roots of Euphorbia fischeriana
Recent advances on bioactive natural products from Chinese medicinal plants
Saving the Ethnopharmacological Heritage of Samoa
Ensuring equitable benefits: The Falealupo covenant and the isolation of anti-viral drug prostratin from a Samoan medicinal plant
A nonpromoting phorbol from the samoan medicinal plant Homalanthus nutans inhibits cell killing by HIV-1
Prostratin as a new therapeutic agent targeting HIV viral reservoirs
Prostratin: activation of latent HIV-1 expression suggests a potential inductive adjuvant therapy for HAART
Prostratin and bortezomib are novel inducers of latent Kaposi's sarcoma-associated herpesvirus
Nonpromoting 12-deoxyphorbol 13-esters inhibit phorbol 12-myristate 13-acetate induced tumor promotion in CD-1 mouse skin
Antireplicative and anticytopathic activities of prostratin, a non-tumor-promoting phorbol ester, against human immunodeficiency virus (HIV)
Cloning of casbene and neocembrene synthases from Euphorbiaceae plants and expression in Saccharomyces cerevisiae
Activation of latent HIV-1 expression by the potent anti-tumor promoter 12-deoxyphorbol 13-phenylacetate
Toxic phorbol esters from Chinese tallow stimulate protein kinase C
Differential effects of phorbol-13-monoesters on human immunodeficiency virus reactivation
Oases Transcriptome Assembler
tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence
RNAmmer: consistent and rapid annotation of ribosomal RNA genes
annot8r: GO, EC and KEGG annotation of EST datasets
Protein phosphorylation in the delivery of and response to auxin signals
Anti-bacterial activity of Euphorbia fusiformis--a rare medicinal herb
The plastidial 2-C-methyl-D-erythritol 4-phosphate pathway provides the isoprenyl moiety for protein geranylgeranylation in tobacco BY-2 cells
Contribution of the mevalonate and methylerythritol phosphate pathways to the biosynthesis of gibberellins in Arabidopsis
Cloning of casbene synthase cDNA: evidence for conserved structural features among terpenoid cyclases in plants
A cDNA clone for taxadiene synthase, the diterpene cyclase that catalyzes the committed step of taxol biosynthesis
A simple and efficient method for isolating RNA from pine trees
Velvet: algorithms for de novo short read assembly using de Bruijn graphs
QSRA: a quality-value guided de novo short read assembler
Short read fragment assembly of bacterial genomes
De novo bacterial genome sequencing: millions of very short reads assembled on a desktop computer
De novo assembly of human genomes with massively parallel short read sequencing
Large scale loss of data in low-diversity illumina sequencing libraries can be recovered by deferred cluster calling
Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences
OrthoMCL: identification of ortholog groups for eukaryotic genomes
Fast and accurate short read alignment with Burrows-Wheeler transform
The Sequence Alignment/Map format and SAMtools
Primer3 on the WWW for general users and for biologist programmers


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VMware 12.5.3 has been released, and release notes are here: http://pubs.vmware.com/Release_Notes/en/workstation/12pro/workstation-1253-release-notes.html Although these notes only mention Kernel 4.9.0, I can confirm that it also supports 4.10.x Kernels (tested on 4.10.1). I applied the vmmon and vmnet patches for Kernel 4.11, … Continue reading
Tag Archives: kernel
There is a fix/patch available now, for NVIDIA driver 378.13 on Kernel 4.11, but it involves modifying the licence on NVIDIA code, and so is not legal.. Details – and links to discussions – here: https://devtalk.nvidia.com/default/topic/995636/?comment=5101210 Some previous changes to … Continue reading
Thanks to Heiner Kallweit for info on two USB patches for the Odroid C2, and thanks to John Youn for the patches themselves.. They can be found at http://www.spinics.net/lists/linux-usb/msg151687.html and http://www.spinics.net/lists/linux-usb/msg151688.html . I have applied these to Kernel 4.10-rc4 (including … Continue reading
Just updated the Odroid C2 to Kernel 4.10-rc4, but the Ethernet hang problem still persists.. Applied the dts Ethernet TX patch (see http://rglinuxtech.com/?p=1872 ) , and it works OK now.. There seems to be a bit of a backlog of … Continue reading
There is now an 'official' fix for the 4.10-rc1 compile failure with ARM64/aarch64, from Al Viro, and the patch itself is available here: https://git.kernel.org/cgit/linux/kernel/git/viro/vfs.git/commit/?h=arm64-fix&id=b4b8664d291ac1998e0f0bcdc96b6397f0fe68b3 I have applied this to kernel 4.10-rc1 on the Odroid C2, and the kernel compiles successfully.. … Continue reading
Recent versions of the Linux net-next tree could not be tested on the C2, as the meson-gx mmc driver was missing, but now it has been added, and I tested the latest version (December 17) – but the Ethernet hang … Continue reading
NVIDIA driver 375.26 has been released, and details are available here: http://www.nvidia.com/download/driverResults.aspx/112992/en-us I had been experiencing some odd window (KDE5) behaviour with 375.20, and hopefully things might be more stable with this version.. If not, then possibly kwin is to … Continue reading
Kernel 4.9 has been released, and (brief!) details of changes are here: http://lkml.iu.edu/hypermail/linux/kernel/1612.1/01831.html The latest NVIDIA (375.20) driver works OK, and VMware 12.5.2 with the vmnet and vmmon hack/patches, also works OK. Hack/patch details are at http://rglinuxtech.com/?p=1838 On my Intel … Continue reading
The old meson Ethernet fix/patch/hack no longer applies cleanly to the latest (December 1) linux-next, but – fortunately – there is a 'better' patch available for the meson8b-dwmac driver itself.. Details can be found in this thread: http://lists.infradead.org/pipermail/linux-amlogic/2016-November/001675.html I have … Continue reading
Just tested the latest NVIDIA driver – 375.20 – and it compiles cleanly with Kernel 4.9-rc5: ……………………………… Building modules, stage 2. MODPOST 4 modules CC /home/rgadsdon/kernel/NVIDIA-Linux-x86_64-375.20/kernel/nvidia-drm.mod.o LD [M] /home/rgadsdon/kernel/NVIDIA-Linux-x86_64-375.20/kernel/nvidia-drm.ko CC /home/rgadsdon/kernel/NVIDIA-Linux-x86_64-375.20/kernel/nvidia-modeset.mod.o LD [M] /home/rgadsdon/kernel/NVIDIA-Linux-x86_64-375.20/kernel/nvidia-modeset.ko CC /home/rgadsdon/kernel/NVIDIA-Linux-x86_64-375.20/kernel/nvidia-uvm.mod.o LD [M] /home/rgadsdon/kernel/NVIDIA-Linux-x86_64-375.20/kernel/nvidia-uvm.ko CC … Continue reading

subset: commoncrawl


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Great set of plastics
By Dave on August 30, 2016
Never expected aftermarket plastics to be as durable as the originals, but these are just as rugged, if not better in ways. It did take a little squeezing and pulling, but the overall fit is on point. Extremely satisfied with UFO's quality.
- Value:
- Quality:


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There is no greater resource for Ohioans with disabilities than the partnerships that OOD has created with outstanding service providers across the state. Working together, with the support of federal funds allocated for service to citizens with disabilities, OOD and partners are able to help clients find employment, independence and benefits.
Jobs | Partner State Agencies | Notices | Rules | Privacy Notice | Accessibility Statement
150 East Campus View Boulevard
Columbus, Ohio 43235
1-800-282-4536 Long Distance
614-438-1200 Local


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Precious "BabyGirl" Wright,
It's been just over a week now since you left us with broken hearts and missing you so much. I want you to know that letting you go was the hardest decision that we ever had to make. We tried so hard to find out what was wrong, but we knew you were in so much pain. BabyGirl, we tried to do everything that was possible to make you feel better again. We didn't want to let you go until you told us it was time. You finally told us. That look you gave us that day was a look that we'll never forget. We knew then that it was time to let you go rest. I'm sad and happy at the same time. Sad that I lost my babygirl but happy that you're not suffering anymore.
Thanks for the 14 years you gave us, we cherished every moment. You've been the best cat ever. I still remember when I first saw you at 6 weeks old. I just went to visit a friend who happened to be moving. I didnt even know you existed until something jumped in my lap. It was you wanting attention. My friend immediately told me that If I wanted you, I could take you home. Thats just what I did and have cherished every moment of it. There was just something special about you. I know it wasnt easy being a military cat. You've seen your share of pet hotels while mommy and me were deployed to Iraq . It was worth every penny to keep you safe until we returned. When we both returned from Iraq, it was like we never left you. I know that Maddie and Lilly miss you so much, but they knew that you were sick. You had such a great disposition, and so friendly. Everyone loved you and always wanted to hold you. Maddie and Lilly loved you so much and loved playing with you.
I know you're in a better place now that you have crossed that beautiful rainbow bridge . You're healthy again with no more pain and suffering. You'll always remain in our memories and will always be our little baby girl. You will always have part of our hearts. You can never be replaced.
Please sign the guestbook for Precious by clicking here
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Sep 1, 2011
Wireless Human Health Online Monitor ECE Project
This is a good Electronics and communication project report on wireless human health online monitor. This project creates a portable device implementing wireless technology and taking full advantage of the wide-spreading Internet to provide a convenient solution to monitor human health. The system provide most convenient method to examine human health by measuring the heart rate and the temperature. You can also Subscribe to FINAL YEAR PROJECT'S by Email for more such projects and seminar.
The health information acquired on the portable side transmits to the server wirelessly and can be accessed all over the Internet in real-time. The end product turned out to be very portable and the wireless connection is responsive and solid. This report includes hardware, software requirement and detail, result and conclusion. Use this report only for your reference and study. Thanks to the author.
Author:-Xun Yang, Yinglei Wang & Houman Azemati
About : Final-yearproject.com
Final-yearproject.com is one stop for all your project needs. You can find projects and seminars related to Electronics, Electrical, Civil, Mechanical, MBA, Pharmacy, Computer Science, IT, Bio-Tech, Robotics, Telecom, Wireless, Automation, BCA, MATLAB etc. You can Like us at Facebook and follow us on Twitter and Google+.


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Embroidery designs on dance dresses were originally of traditional Irish origin, many coming from the Book of Kells and Irish stone crosses. The knotwork in the pattern on Rinceoiri's new dress is said to symbolize the continuity of the friendships made in the group.
The creation of new dresses marks the 15th anniversary of Rinceoiri Don Spraoi Irish Dancers in Utah.
Where Irish dance comes first!


text: Sometimes while typing on websites in large textareas my screen won't show what I am typing. It will only show a sliver of the page and the keyboard and the rest is black. Now I am not sure if this has to do with the lag that is associated with typing or not, while on websites and typing I sometimes can type entire sentences before I even see the text show up and then once it catches up I see the screen scroll way down and go black. Does anyone else either experience this or know why it is caused?


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[Task] Hotgas nozzle with Pointview
2 Attachment(s)
Dear Forum Users!
After completing some simple meshing tasks with gmsh (2.2.6) and discretizer, I will do some more realistic meshing cases. I have to use this old gmsh version, otherwise there is no chance for me to convert it via gmshToFoam (OpenFOAM 1.5-Dev).
The next case will be a more realistic variant of my hotgas nozzle (inert gas). We have different wall temperatures and an inlet pipe, which is normal to the main pipe. Later on, I wanna take a turbulent heat transfer solver.
For a better imagination, I attached two images of the 3D part (ProEngineer). For the first try I exported it as a .stl
The colored surfaces represent the intended boundary layers:
My experience with snappyHexMesh is ... not the best. I dont know how to define the boundary layers and there are problems with "sharp" edges. Maybe there is a way with the discretizer...
Importing the .stl in gmsh 2.2.6 works really good, but I dont have an idea how to create the 3D mesh with this version.
So, do you have some ideas, how to get a mesh out of the 3D model for the upcoming case.
Thanks in advance
Edit: Just work around with Pointwise and updated the 3D model to be more ... fluent ;)
I split the geometry in a rear part with the intersection, and a front part with the nozzle, if someone have experience with this kind of meshing, help is welcome :o
Its going on
1 Attachment(s)
Nozzle Version 2 - see last post:
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VIDEO: Patrick Swayze's DWTS Tribute
The highlight of last night's Dancing With The Stars elimination ceremony was beyond a doubt the touching tribute to Patrick Swayze.
Six of the show's professional dancers were tasked with dancing the tribute medley to the late Dirty Dancing hunk, who passed away from pancreatic cancer on September 14 at the age of 57.
Chelsie Hightower and Dmitry Chaplin danced to "She's Like the Wind," while Jonathan Roberts and Anna Trebunskaya waltzed to "Unchained Melody."
Tony Dovolani and Cheryl Burke ended the tribute with the amazing "Time of My Life" and that final lift into the air from the end of Dirty Dancing.
Swayze was also a guest mentor on the first season of Dancing With The Stars in 2005, which makes the tribute even more fitting.


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Home · Website Design · Hosting · SEO · Graphic Design · Portfolio · Contact
Stranraer FC, Stranraer, Scotland
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'Kotalik ranked third on the team in goals behind Miroslav Satan (26) and Daniel Briere (21). He was named Rookie of the Month for January after recording seven goals and four assists.'
Note: While we are busy tweaking the front page design to make it more appealing and functional (especially for our mobile readers), you may notice odd layout errors. Please hit "control-F5" to refresh the page and load the latest files. Thank you!


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new.body.electric Salt Lake City, Utah
NBE was formed in the great city of salt - near a lake, in Utah. As if fate itself pulled the reigns of these four artists together, they collided in an explosion of face-melting grooves, and hair-raising melodies. Their upbeat and unique style will surely turn the heads and ears of many as this tiny snowball begins it's momentous musical journey. ... more
Streaming and Download help


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Etek Europe Ltd., a leading supplier of products and services to the European electronics industry, are proud to announce the installation of a Nordson DAGE Diamond Flat Panel with X-Place and CT Options to NI Hungary.
Nordson DAGE, the only X-ray company whose focus is on X-ray electronics inspection, offers the Nordson DAGE XD7600NT Diamond FP X-ray inspection system that uses the latest technology, flat panel detector to provide the ultimate choice for the highest quality real time X-ray imaging. The unique Nordson DAGE NT maintenance-free, sealed trans missive X-ray tube, providing 0.1 µm feature recognition and up to 10 W of power, together with the 3 Mpixel long lifetime CMOS flat panel detector, makes this jewel of a system the choice for the highest performance and highest magnification real-time imaging.
Gheorghe Rebegel, Etek's Sales Manager in Eastern Europe, said "ETEK-Europe are very excited to have successfully closed such a demanding project which has further strengthened our partnership with NI Hungary.
This project was a huge challenge for our sales and application teams who received high levels of support from NI Hungary's excellent team of engineers."
National Instruments transforms the way engineers and scientists around the world design, prototype and deploy systems for test, control, and embedded design applications. Using NI open graphical programming software and modular hardware, customers at more than 30,000 companies annually simplify development, increase productivity and dramatically reduce time to market.
Etek Europe has a strong reputation for being a quality provider of products and services to the aerospace, automotive, defense, medical and European electronic manufacturing industries.
With over 1,300 customers, Etek has built its success by developing strong relationships with both customers and suppliers, which ensures it continues to deliver premium solutions at a highly competitive price.
Together with one of the largest installation and service teams in the UK and with thousands of successful installations completed, Etek's engineers have developed invaluable and unrivalled product knowledge and experience in all aspects of planning, supply, installation, testing, commissioning, maintenance and training.For more information about the products and services provided by Etek Europe please visit www.etek-europe.com.


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Jan 28 (Reuters) - HINO MOTORS LTD CONSOLIDATED FINANCIAL HIGHLIGHTS (in billions of yen unless specified) 9 months ended 9 months ended Year to Dec 31, 2008 Dec 31, 2007 Mar 31, 2009 LATEST YEAR-AGO LATEST RESULTS RESULTS FORECAST Sales 882.79 1.00 trln 1.10 trln (+7.9 pct) Operating loss 4.49 prft 37.14 loss 13.00 (+30.5 pct) Recurring loss 12.57 prft 35.28 loss 23.00 (+19.8 pct) Net loss 19.54 prft 19.31 loss 33.00 (+26.5 pct) EPS loss Y34.07 prft Y33.64 loss Y57.65 ...
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- Product Details
Look carefully at our Personalised Luxury Hip Flask - Vintage Whiskey and you will see the styling matches perfectly with your whisky lover's favourite brand, ensuring this gift is a hit from the very start.
Along with our "Vintage" caption that has already been expertly engraved on the front of the flask, there are a further five lines of text for you to choose. All of which will be fitted into this fabulous design.
Make your favourite whisky drinker's birthday a dream come true. Or give this unique present to your Best Man and put his favourite blend inside.
The gift comes with its own coordinating pouring funnel, and is then presented in a very attractive gift box.
For your Dad on Father's Day or your Aunt as she takes to the slopes, this is a present that is going to be enjoyed over and over again.
- Luxury 6oz black hip flask
- Expertly engraved
- Easy filling funnel included
- Presented in a gift box
- Measures approx. H:9.5cm , W:9.5cm, D;2cm
- Delivery & Returns
We are aware of how important it is for you to receive your gifts in time. Therefore, once you place your order we will process straight away and dispatch as soon as they are ready to go. We usually dispatch all items within 24-48 hours, however, this may be a little longer during special occasions such as Mother's Day, Father's Day, Christmas, etc. If multiple items ordered, we may dispatch items separately or all together, depending on the process.
*2nd CLASS ROYAL MAIL
Sent via 2nd Class Mail for only £3.99.
*1st CLASS ROYAL MAIL
Sent via 1st Class Mail for only £4.99.
Sent via Royal Mail Tracked for only £5.99.
Orders received by 2pm, sent via next working day courier tracked service for only £7.99.
Orders received by 2pm, sent via Royal Guaranteed Saturday Delivery for only £9.99.
The cost for delivery to European countries is calculated based on the destination. Please allow approx. 2-5 working days from dispatch, however, this is not guaranteed.
Below areas will incur a remote area surcharge - £30
*Italy: Livigno & Trepalle, San Marino, Vatican City
*Spain: Ceuta, Melilla
*Finland: Aland Islands
*Free delivery excludes items that are on offer or discounted from the original price and sent via 2nd Class Royal Mail, only UK.
*2nd Class Royal Mail - Royal Mail aim to deliver within two to three days, however, this is not guaranteed by Royal Mail, only UK.
*1st Class Royal Mail - Royal Mail aim to deliver your order one to two days, however, this is not guaranteed by Royal Mail, only UK.
*Premium Tracked - Royal Mail aim to deliver your order the next day, however, this is not guaranteed by Royal Mail, only UK.
*Next Working Day - All Orders placed before 2pm will be delivered next working day (Excludes Northern Ireland, Overseas UK addresses, and Parts of Scotland).
*Saturday Delivery - All Orders placed before 2pm will be delivered Saturday (Excludes Northern Ireland, Overseas UK addresses, and Parts of Scotland).
*All our items require production time and this is stated on individual product pages.
We're sure that you will be satisfied with your purchase and we also understand that sometimes you may want to return. We keep this process simple! Just contact us if you wish to return goods. Please note: personalised, made-to-order and perishable items are non-refundable.
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Desert Gems
457 Wadsworth Blvd Ste A, Lakewood, CO 80226
(303) 426-4411
457 Wadsworth Blvd Ste A, Lakewood, CO 80226
(303) 426-4411
Regular Hours
Phone: (303) 426-8309
Parking: Lot, Private
Bike Parking: No
Wheelchair Accessible: Yes
By Appointment Only: No
No coupons available
Be the first to add a photo!
I wish I could give them no stars. The only thing this shop has is a poor selection and the women have horrible attitudes. I have been in a few times because at first I thought maybe they were just having "off" days, unfortunately that's not the case. They are just shockingly negative women. I prefer to give my business to people who don't treat me like I'm inconveniencing them with my simple questions. To top it off the prices are outrageous. I Recommend shopping elsewhere before giving them any business.
I visited this store today and the employees were kind and helpful. One even did a bit of online research for me while I shopped! Nice selection and overall the prices seem reasonable.
I have been to Desert Gems several times and have always had good customer service. The gemstone bead selection is pretty good and they have both base metal and sterling findings. The beads behind the counter can be a little pricey. Overall, this is a good store.
I'm in the market for a rock tumbler and knew about this store so I checked their website to see what they had. It merely listed that they had tumblers but didn't show the prices. I called and spoke with a woman who acted like I was troubling her by calling. After that experience and reading the reviews here I won't be going to this store. Why is good customer service so rare these days?
Went into the store and was treated with complete disrespect from the employees. I have been in sales for years and never treated so horribly. They don't care to answer any questions or help you with a purchase what so ever! Go somewhere else for your gifts don't support this business. In this economy you have to learn to treat your customers with respect or you won't have a business.
12/30/2008Provided by Citysearch -
Horrible customer service. Manager rude and not repsonsive to concerns. Was told she could care less if I shopped there or not. Requested I be able to speak with the owner and was told he lives in Peru!


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On 10/13/2011 02:56 AM, Daniel Veillard wrote:
On Wed, Oct 12, 2011 at 05:55:16PM -0600, Eric Blake wrote:358 changes; most probably have no impact on libvirt, but we might as well stay current. * .gnulib: Update to latest.Sure, ACK,
Thanks; pushed. -- Eric Blake eblake redhat com +1-801-349-2682 Libvirt virtualization library http://libvirt.org


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Genuine Fractals v6.0 Professional | 248 MB
Genuine Fractals 6 is the industry standard for image resizing. It is renowned across the photographic and printing industries for its ability to increase image size over 1000% without the loss of sharpness or detail that you would normally expect. Its patented, fractal based interpolation algorithms work like nothing else and the results speak for themselves. Genuine Fractals 6 allows you to crop and resizing your image in a single step with no guess work. Its new Tiling and Gallery Wrap features makes it easy to get your enlargements printed just the way you want. Fire up the batch processing engine to re size an entire folder of images quickly. You can even access the power of Genuine Fractals inside of Adobe Photoshop Lightroom or Apple Aperture.
What's New in Genuine Fractals 6 Professional Edition
• Gallery Wrap Feature - When making enlargements for canvas, it is common to wrap a portion of the image around the wooden stretcher bars, creating a gallery wrap. However, when wrapping an image in this way, important detail can be lost near the edges. The new Gallery Wrap feature in Genuine Fractals 6 Professional Edition automatically creates extended margins by reflecting or stretching the areas near the edge of the image allowing you to create a gallery wrap without sacrificing any of the original image.
• Tiling Feature - Now you can get large format printing from a small format printer, quickly and easily. The new Tiling feature divides an enlargement into smaller pieces, preparing it for printing on a standard-sized printer. Just specify the paper size and overlap option and Genuine Fractals 6 will automatically resize the image and create individual tiles that are ready for printing.
• Batch Processing - New in Genuine Fractals 6 is an improved version of the onOne batch processing engine. This allows you to resize an entire folder of images to a consistent size and create up to two new versions of an image. You can control the size, resolution, file formats, name and location, color space, and even add a watermark logo.
• Photoshop Lightroom & Aperture Integration - Genuine Fractals 6 plugin can now be used inside of Lightroom and Apple Aperture, in addition to Adobe Photoshop.
• Texture Presets - Genuine Fractals 6 now features texture presets for common image scenarios so even a novice user can get improved texture control results easily.
Genuine Fractals 6 is available in two versions, Professional Edition and Standard Edition. Both editions offer you the same award-winning, patented image resizing algorithm that has made Genuine Fractals famous. The Professional Edition adds several new features that make it a must-have for professionals or serious amateurs. These include Photoshop Lightroom and Apple Aperture compatibility, support for CMKY color mode files, and the new Gallery Wrap feature for printing on canvas.
- Photoshop CS2(v9.0.2), CS3, or CS4
- Photoshop Elements 4 or later
System Requirements (Windows):
- Windows XP SP2 or Vista
- Microsoft .Net 2.0 Framework or higher
- 512MB of RAM
- 25 MB of disk space
- Adobe Flash Player 9
homepage - http://www.ononesoftware.com/detail.php?prodLine_id=7


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MAGNETIC Radio 004 by John Cutler
John Culter presents next episode of MAGNETIC Radio podcast. This new episode of MAGNETIC by John Cutler brings you his in-deep mix skill of electronic vibe. Supported by leading dance music event in Central Europe, MAGNETIC Festival. This mix is available for free download.
Title : Magnetic Radio 004
Artist : John Cutler
Genre : Electronic / House


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Esmerelda, circa 1900
You may know that we bought a house as a hobby project. Some people collect stamps, others knit, but we've taken on renovation of a century-old house. We're somewhat embarrassed that, even though our house once belonged to the former head … Continue reading
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Evergreens are trees and shrubs typically prized for year-round, colored foliage. It may be alarming if an evergreen tree's needles drop, turn brown or yellow before dropping or a tree begins to look bare over time. Several factors could be responsible for this needle drop, ranging from natural seasonal conditions to chemical damage, environmental stresses or others.
Natural Seasonal Needle Drop
Evergreen trees retain year-round coloration and foliage because needles typically last for several years while the tree undergoes a gradual, continuous needle drop. Most species see a spike in the amount of needles dropped during a certain season; the majority of evergreens have this peak in fall. Most of the needle loss occurs on the older, innermost sections of branches; white pines typically have the most dramatic seasonal yellowing and drop.
Needle drop may be caused by an infestation of spider mites. Affected needles suffer stippling and eventually turn yellowish. A silky webbing may be present. Test for mites by shaking a branch while holding a white sheet of paper underneath. The mites will appear as small moving dots on the paper.
Inadequate Water or Nutrients
Excessive needle shedding and poor growth may result from drought. Clay soils may break fine roots as the soil dries and shrinks away from roots; well-drained sand and gravel sites can exacerbate drought conditions during hot weather. Insufficient nutrients can also cause needle discoloration and drop. The most prevalent nutrient deficiencies are generally lacks of nitrogen or iron, which first cause the oldest tree needles to turn yellow.
Herbicide Damage
Damage from errant herbicide applications or other chemicals can cause needle distortion. A common symptom of herbicide exposure is tip damage on new growth, needle distortion or browning. Spruce needles will turn purple and drop. Exposure to deicing salt is a common cause of injury to roadside trees. Needle yellowing or browning at the tips precede needle drop. Air pollution can cause narrow yellow bands to develop on needles, tips to turn brown or a severe reduction in growth.
Poor Drainage
Poorly draining sites with excessive water can reduce the amount of oxygen available to the tree roots. Root suffocation will eventually manifest as needle yellowing and drop and poor growth. Construction or similar activities can compact the soil and lead to poor drainage conditions. Saturated roots are also susceptible to infection by the fungi that cause root rots.
Establishment Issues
Recently transplanted trees may suffer decline or stress for several years after transplant. If the roots were not properly freed or were disturbed at planting, support wires remain on the tree for too long, the tree is being watered improperly or it was planted too deeply, needle loss can occur.
Low Light
Most evergreens require full sunlight to thrive, so low light can cause a slow decline and foliage drop beginning in the center of the tree. This condition is most likely to occur if the tree was planted in a poor, shaded location or is adjacent to trees that have grown large enough to cast a shadow on the evergreen.
Related Searches
- Utah State University Cooperative Extension; Needle Drop of Evergreens; Sherman V. Thomson and Scott C. Ockey; January 2000
- Ohio State University Extension; Yellowing, Dieback and Death of Narrow-Leafed Evergreens; Stephen Nameth, et al; June 1996
- University of Saskatchewan Extension Division; Browning of Evergreens Can Be Controlled; Grant Wood
- Photo Credit Jupiterimages/Comstock/Getty Images
You May Also Like
Types of Long-Needle Evergreen Trees
The leaves of many kinds of conifers take the form of spindly, needle-like structures. Those of most spruces, firs and hemlocks are...
Mites in Evergreen Shrubs
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Let's say a guy approached you at the gym or store or bowling alley. If he was a sloppy kisser, and his therapist had given him the homework of making out with 5 different girls, would you be willing to make out with him for a couple minutes and give him feedback on how to make it better if he asked nicely?
Most Helpful Guy
Did your therapist give you this as homework? I would probably keep that part to yourself, to be honest. No judgment, mind you. But I wouldn't walk up to a girl and be like, "hey! So my therapist game me some homework and I wondered if you'd help me..."00|00|0


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'Mad Men's' John Slattery Joins 'Arrested Development'; 'Big Bang Theory' Alum Guests on 'Suits'
Mad Men star John Slattery is headed to Arrested Development for a few episodes when the cult show's fourth season premieres on Netflix. Details regarding his role are unavailable at this time. All 10 episodes of Season 4 will premiere at once when the show hits the streaming site. [TVLine]
Which The Big… More »


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Powerful AC motor with auto-reset eliminates motor burnout Designed for heavy use Sharpens all standard-size pencils Pencil Saver™ prevents over sharpening Quiet operation, perfect for busy areas Overall size: 4-1/4Hx3Wx6-1/2"D Manufacturer's 2-year limited warranty Made in USA
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Overview Edit
The Emergency Management Response System is used to manage investigations of outbreaks of animal diseases in the United States. This Web-based task management system was designed to automate many of the tasks that are routinely associated with disease outbreaks and animal emergencies. The system is used for routine reporting of foreign investigations of animal disease, state-specific disease outbreaks or control programs, national responses, or natural disasters involving animals. The system also has a mapping feature, which allows for real-time identification of outbreaks to enable responders to respond more quickly by providing high-resolution maps to decision makers, government agencies, and the public. The system interfaces with state and federal diagnostic laboratories for reporting test results.


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Decision makers
- Scott Brown
Tell Scott Brown: We don't want your Big Oil politics in New Hampshire
As the senator from Massachusetts, Scott Brown made lots of friends – unfortunately most of them were dirty energy industry lobbyists rather than the people he was supposed to represent. Brown took thousands from Big Oil just weeks before voting to keep giving oil companies billions of our taxpayer dollars in subsidies every year.
Now Scott Brown is shopping for a Senate seat in New Hampshire. Good for Big Oil. Not so good for us!
If you want Scott Brown to keep his Big Oil politics out of New Hampshire, add your name to our petition today.
- Scott Brown
As voters in the state of New Hampshire, we'd like to spare you the trouble of running and tell you now – we don't want your Big Oil politics in our state. We know that as the senator for Massachusetts, you voted not just once, but THREE times to keep giving oil companies billions in taxpayer-funded handouts every year.
We don't want a senator in New Hampshire who will put polluter interests above his own constituents, making us pay twice for our gas – once at the pump and again on tax day. So please stay out of our state and take your Big Oil agenda with you.
League of Conservation Voters started this petition with a single signature, and now has 1,517 supporters. Start a petition today to change something you care about.
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I know you say round skipping will never work again in lockdown extreme, but I was just in the game and saw someone in my group do it for us.. it is like the round just kept resetting and before you know it, we were at round 18 and done. It was wild, so someone has figured out a way. It looked something like going in and resetting the game looks like sometimes.
Any ideas?
Last edited by klaw555; 07-15-2013 at 04:55 PM.


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
You may also want to note that you are unable to download the EncryptedAsked by pigglywigglymidwest on September 07, 2016 at 01:41 PM
You may also want to note that you are unable to download the Encrypted submissions to Excel.
This is a re-post of a comment on Encrypted Forms and How to Use Them
JotForm Support
That is correct. It is not possible to download encrypted submissions in excel. You can find this on the bottom of the guide Encrypted Forms and How to Use Them.
Some of the features that are not available for Encrypted Forms* are:
1. PDF Downloads,
2. Reports (this includes downloading Excel and CSV files from within Submission Panel),
3. Emails that contain form data in them.
* All server side gathered and processed data can only be retrieved in encrypted form because your private key is never sent to our servers.


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LONDON—She was a beautiful English princess who married one of Europe's most powerful monarchs and dazzled subjects with her charity and charm.
Now a team of British and German experts say they think they've found the body of Princess Eadgyth (pronounced Edith) — a 10th-century noblewoman who has been compared to Princess Diana.
"She was a very, very popular person," said Mark Horton, an archaeology professor at Bristol University in western England. "She was sort of the Diana of her day if you like — pretty and full of good works."
Horton is one of a team of experts working to verify the identity of some bones found bundled in silk in Magdeburg Cathedral in Germany.
Should the skeleton be positively identified as belonging to Eadgyth, it would be oldest remains of any English royal discovered so far. Experts say her closest competitors — the bones of various Saxon royals in Winchester Cathedral in southern England — are so hopelessly jumbled together that no single person can be identified.
"If (Eadgyth's) skeleton is intact then, yes, as far as I'm aware, it would be the earliest identifiable remains from Anglo-Saxon England," said Simon Keynes, a professor of Anglo-Saxon history at the University of Cambridge.
The skeleton was uncovered as part of a wider research project into Magdeburg Cathedral, about 90 miles (150 kilometres) west of Berlin. The elaborate 16th-century monument in which her body was found was long thought to be empty.
When archaeologists opened the monument in 2008, they found a lead coffin bearing her name and carrying a nearly complete set of bones wrapped in silk.
Horton said the skeleton belonged to a woman between 30 years and 40 years of age. But there is some doubt it is the late royal: Historians believe Eadgyth's body was moved at least once — and may have been replaced in the tomb with those of another person.
"Quite often they scooped up any old bones and put them in," Horton said.
Tests will now be performed to figure out the age of the bones and where they come from. Among the techniques being used is strontium isotope analysis — a technique that measures the mix of strontium isotopes in a person's tooth enamel to determine where they grew up.
Because different mixes of the trace metal correspond to different geographic locations, the technique can help pinpoint where a person lived.
"Strontium is the smoking gun if you like," Horton said.
Gareth Williams, a curator at the British Museum, said the technique was "fairly highly regarded" among archeologists and should be able to show whether the skeleton's owner grew up in Germany or England, for example.
Keynes didn't know much about that technique but said if the skeleton could be shown to come from western or southern England there would be little doubt it was Eadgyth.
"If you can tell that scientifically, then wow," he said.
Eadgyth grew up at the dawn of the 10th century, a period during which her half brother Athelstan extended his rule over all of England and drew on his sisters to cement his influence among foreign rulers.
"He's well known for having a superfluity of half sisters, and he married them off to the ruling houses of the rest of the known world," Keynes said.
Eadgyth was destined for Duke Otto of Saxony, a warlord's son who would eventually rise to become the first ruler of the Holy Roman Empire.
Tradition holds that she and her younger sister Adiva were both presented to Otto, who was invited to pick which one he liked best. Eadgyth's looks and charm won out over her sister's youth.
Keynes groaned when asked whether Eadgyth could be compared to Diana, whose marriage to Prince Charles in 1981 captured the world's imagination.
But then he read from the chronicle of Hroswitha of Gandersheim, a contemporary of the princess, who said Eadgyth was "resplendent with a wondrous charm of queenly bearing."
Then followed a particularly florid passage in which German nun writes: "Public opinion by unanimous decision rated her the best of all women who existed at that time."
Keynes came around.
"Now I remind myself of that, what can I say?" he said. "She certainly fulfilled for them the function that the Princess of Wales fulfilled for many Britons."
Eadgyth bore Otto at least two children, but like Diana she died young. Both died at age 36.


text:
What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Default value not appearing in when marked as required fieldAsked by NRCsupport on February 17, 2014 at 08:14 AM
I have set up a numeric field to take values 1 to 8 with a default of 1. If I make the field required, the default value is not put into it when it opens. If the field is not required the default value does appear.
Is this a bug or a deliberate feature?
I checked on this via a separate test per what you stated above but I didn't experience the same issue. It sounds to me like this might possibly be a minor glitch on your form. I would advise clearing your form cache then check again to see if this makes any difference.
Should you still have issues with this then may we please have a link to your form which has this field on it so we can troubleshoot it further for you.
Thanks, yes it is ok now. It may be relatedto my problem with a spinner field, discussed in another thread.


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A BID has been made to scrap plans for a new school and industrial park from a huge housing development.
Developers now hope to build an extra 120 homeson the former Saighton Army Camp, near Huntington, on top of the 800 already granted permission.
Over the years the huge new estate has led to a great deal of opposition, with hundreds of local residents concerned the road network would not be able to cope with the growth in population.
Last August Cheshire West and Chester Council (CWaC) gave permission for 295 homes on the former army barracks despite protests from campaigners who had major road safety concerns.
Residents argued the Caldy Valley junction – formerly known as the Sainsbury's roundabout and now dubbed the 'hamburger' roundabout – was already dangerous and the extra houses would create further traffic chaos.
The improvements to the road layout were met with widespread criticism by Chester residents, claiming the new scheme was confusing and a danger to motorists.
A number of local businesses also claimed that traffic delays caused by the extensive improvement works hit them hard financially.
Before permission for the new homes was granted on appeal, Huntington, Great Boughton and Christleton Parish Councils alll objected to the plan on traffic grounds as did Chester MP Stephen Mosley.
But six committee members voted in favour with one against and one abstention.
Developers, Taylor Whimpey, have already built about 100 properties at the site, but are now looking to increase the number of homes built by asking for permission to build on a part of the old army camp which has been earmarked for light industry.
Over the years a number of calls have also been made to build a new slip road, directly onto the nearby A55, to ensure that minor roads in Huntington and Boughton did not become gridlocked with the extra traffic.
Applicants, GMV Eight, originally included a primary school and 5,000 square metres of employment land as part of its vision when it was granted permission, on appeal, for 375 homes on the site.
It now wants to replace the primary school and light industry land with 120 more homes saying there "remains an absence of any demand for employment" and that the school proposal "will not be implemented" due to an alternative location for the new Huntington primary school being preferred.
However ward councillor, Cllr Mark Williams, said the applicant had not provided any evidence that an industrial area was not needed.
He said: "I see from the application online that there is no substantiated evidence that there is not a need for a light industrial area on this site.
"I find this a substantive omission by the applicant as the light industrial area carried considerable weight at an earlier appeal – allowing area B (375 houses) to go ahead.
"Also, an application on the school site could be considered as some what premature."
A condition of approval was that GMV Eight invested £4.6million in travel improvements including a revised Caldy Valley junction and public transport subsidies, gave £653,000 for improvements in schools, made 20 per cent of the dwellings affordable housing units and gave recreation land south of Sandy Lane to the council for a nominal fee.
See full story in the Chester Leader


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Barack and Michelle Obama joined dozens of world leaders to pay tribute to Nelson Mandela at his memorial in Johannesburg, South Africa, but the occasion wasn't entirely solemn. Barack palled around with Danish Prime Minister, Helle Thorning-Schmidt, much to the chagrin of First Lady Michelle.
First they took a selfie with British Prime Minister David Cameron. Michelle did not feel the need to participate. Michelle looks more like she's at, say, Nelson Mandela's memorial.
"LA" photo du selfie #MandelaMemorial est l'oeuvre de notre photographe #AFP Roberto Schmidt http://t.co/8MLTh3eoBc pic.twitter.com/POxuT95LHP — Agence France-Presse (@afpfr)
December 10, 2013
Then there was some kind of excited discussion. We're not sure what it was about, but it involved a lot of hand gestures. PHOTO: Reuters.
Then, the touch on the shoulder. Barack and Helle seem to be having a great time. Michelle is not amused.
Is this an isolated incident? Maybe. But then again, Barack Obama and Helle Thorning-Schimdt have met before, and Helle did seem to command Barack's attention…
President Obama speaks with Danish Prime Minister Helle Thorning-Schmidt.
Read More: President Obama Appears To Get Flirty With Thai PM


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|Where's a telephone booth when you need one?
By JAMES KENNEDY
IT WON'T TAKE SUPERMAN to make Providence a functional city. It will take active people, pushing back against bad ideas coming from our elected leaders.
Stephen Miller, a Rhode Island native and journalist for the Streetsblog Network, recently highlighted on his own blog an amazing new hospital in Buffalo, New York. There will be no parking for employees at all. Because of the successful development of a metro rail system within Buffalo, it is now possible for 17,500 new jobs to be created in its downtown without the huge added expense of building parking garages to serve those jobs. In addition to the hospital itself, the rail-oriented development plan has also caused a boom in real estate and mixed-use development in the area, according to The Buffalo News. Miller's article points out that Providence could use the same techniques as Buffalo to make itself attractive to new growth, and outlines specific steps to be taken.
This success in Buffalo should be an added reason to view proposals to knock down The Superman Building as flat-out insane. Buffalo, even more so than Providence (video, see especially 3:32-4:05), is a city that in the mid-20th Century was torn apart by highway-oriented transportation at the behest of people like Robert Moses. Yet, clearly, Buffalo is learning its lesson and turning back on its mistakes. Will Providence?
Go Local Providence reports that Fmr. Mayor Joseph Paolino has already started work to try to save the building, but even his statements give a grim testimony to the way that Providence views its landscape. One of the major problems with the building, he reports, is that it has no parking. The question remains, why should a building that is across the street from the state's public transportation hub have parking at all? Especially when downtown looks like this already? There's a reason that Superman is regarded as being more powerful than a locomotive. Let's keep it that way.
There are two basic views that one can take towards Mayor Taveras' proposal, and I'm not fully committed to either. The first is that the mayor through some fault or error of judgment has actually convinced himself that it would be acceptable to demolish the Superman Building. He states this in a simpering, wishy-washy way, albeit, but it's on the table. Given the car-crazy comments of even people like Paolino who are lobbying to retain the building, it seems possible that Taveras too might be deluded into thinking a new building designed for car commuters would be better. That's flat unacceptable.
The second, and more favorable, view of the mayor is that the he is using the sheer shock of the proposal to make it clear what could happen if we don't act to save the building. I find the mayor's positions on a number of issues very admirable, and he seems like a nice person, so I have tendency towards wanting to believe this second option, if only to give him the benefit of the doubt. However, assuming the best of the mayor in no way means that we shouldn't get on his case and make his political life difficult for as long as is necessary to stop the demolition. I've related already the anecdote of A. Philip Randolph speaking to FDR in the 1930s, explaining the many problems of the Sleeping Car Porters to the president. FDR patiently listened, and said, "I agree, but make me do it". The point of the anecdote is that change doesn't come from good leaders without a fight, and even bad leaders will deliver good policies with a fight.
People should tweet the mayor to let him know that demolition is not an option (and please include us in the tweet with hashtag #AngelSaveSuperman). If you belong to a community group, or can even gather a handful of friends, go out and talk to people about why this is important. This is a transportation issue. Allowing the Superman Building to be demolished for more car-oriented development could be the straw that breaks Providence's back, while a successful push to save the building could be an opening hurrah for making our city more transit and bike oriented.
Usually I'd say, "Let's try not to become Buffalo". But perhaps that advice is out of date.


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- HubPages»
- Holidays and Celebrations»
- United States Holidays
Free Thanksgiving Coloring Pages and Holiday Gift Suggestions
Free Coloring Pages for Thanksgiving
Here are several coloring pages that will pop up as you cursor over them.
In elementary school at Thanksgiving time, we made turkeys in the first and second grades with a potato, toothpicks for legs, and for the tail, wings, and head - cutouts from colored papers.
It was n the first grade classroom, that I learned what a rotten potato smelled like! We had left the potato turkeys over the long Thanksgiving weekend and they did not smell pretty when we returned. Whew! They were fun to make, though. (Now I would worry about wasting a potato.)
The vegetables below make fun coloring objects and can help teach younger children about nutrition. All ages can enjoy the animated characters of onion, potato and carrot. They are all likely to appear on the dining room table for dinner on Thanksgiving or Christmas.
Since potatoes and opinions come in so many colors, children can experiment with their own ideas as they color.
Vegetables can be fun to color.
Coloring Page Resources
- Kaboose
Thanksgiving Coloring Pages and pages for many recognized holidays and activities.
- Unique Thanksgiving Coloring Pages for America
At Thanksgiving, let's present additional high quality coloring pages for the kids that picture more about real-life American History than they may have been taught in school. We know that there were no such things as ...
Children's Art Resources
- Ohio's American Made Products -- Gifts For Special Occasions
Ohio was an agricultural state long before other industries took hold. The capital of Columbus is still popularly called a Cow Town. However, the city is known also for the arts, arts education, and Ohio Made gifts.
- The Toys of Ohio Art Company
We thought Ohio Art Company would close down and take our favorite toys with it, but it was all saved!
Gift Giving Seasons
When Christmas and Kwanzaa rush onto your calendar just around the corner from Thanksgiving, they, along with other Fall and Winter Holidays, can be stressful and challenging. You may feel pressed to purchase the "right": gifts for adults and children in your life.
While it is admirable to abstain from inordinate consumerism, the following toy suggestions make useful as well as entertaining gifts for children.
You might be interested in the Classic Toy Museum.
Do you remember any of these? --
Ohio Art Company - Made in America
- Colorforms - This set of colorful two-dimensional plastic cling items to use for making outdoor or indoor scenes, cartoons, and doll outfits has been a favorite of many children for a long time. While convalescing from pneumonia as a child in the hospital, the staff gave me a large set of Colorforms and a board on which to place them (they clung instantly, but came off easily). The artistic toy helped me forget that I was feeling sick and it was a big surprise for me that I was allowed to take it home when I was released.
- Viewmaster
- Mr. Potato Head
- Tinker Toys
- Ant Farms
- Wooly Willy
- Wheel-Lo
How about Magna Doodle and Etch-a-Sketch?™
That company is still around and operating n Ohio. Ohio Art opened in 1908. Today, they also make lithographic parts for large corporations like Disney, Starbucks, Altoids and Coca-Cola. See the links below for retro toys from Ohio Art.
The Old Time Candy Company
Candy from the early 20th century is also still dsitributed in Ohio at the Old Time Candy Company.
This corporation puts together gift packs for gift giving as well as individual orders. It's fun just to look at their online catalogue. I've never seen some of these candies before, but my parents, grandparents, and great grandparents surely enjoyed some of them.
They even have FIZZIES!
Old Time Candy Company
- Website: http://www.oldtimecandy.com/
- Street Address: 350 Commerce Drive East; LaGrange, OH 44050
- Fun phone Number: 1-866-WAX-LIPS (866-929-5477)
Old Tyme Candy Company
Show with distances in
Thanksgiving Decor of Natural Materials
During October in Canada and November in USA, we speak of Indian Corn, the number of different varieties of corn grown when the white settler met the Native North Americans for the first time. Ears with many colors on the same cob are often wired together and hung on the front door of the home as a welcome during the Thanksgiving Season.
Add Some Color to this Indian Corn!
© 2007 Patty Inglish
coloring rocks
Ahhhhh Patty!! What a glorious Hub!! Thank you!! Thank you!! Thank you!! In my next life I am coming back with your Internet skill, words of wisdom, creativity and imagination!! I am giddy like a child!! I had no idea so much existed out there in Neverland!! You are a jewel and an inspiration!! Blessings, Earth Angel!!


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Officer Valentine is an active duty police officer somewhere in the Midwest United States. His background in law enforcement and in the Marines lends particular credibility to his critique of the shooting of Michael Brown by Officer Darren Wilson.
"Officer Valentine" is, of course, a pseudonym used to protect the identity of this brave police officer who has come forward and allowed us to interview him. Barney Cox of Cop Block conducted the interview below. We at Counter Current News do not necessarily agree, nor disagree with the responses of Officer Valentine, but we consider his perspective and voice to be an important one.
Unfortunately, voices like his are marginalized and silenced by others in his profession, forcing him to conduct this interview in an "underground" manner. We asked him, fairly open-endedly, to comment on the shooting of Michael Brown by Officer Darren Wilson. This is what he had to say…
To understand what happened, you have to think about the probable mindset of the two individuals.
I don't think Michael Brown ever imagined he'd be shot
I also don't believe Darren Wilson ever imagined he'd draw his weapon.
If we look strictly on the narrow basis of whether Wilson's use of force was justified, NOT looking at the whys and wheres of how he got to the point where he HAD to use that force, I'd say, as a police officer, I am satisfied that what he did was legal and justified.
To an officer, squad car is a tomb when you're in a fight. You gotta get out of it or be prepared to die in it. And when you're pinned down by a 300 pound man…damn right I'd shoot.
And if he doesn't get down and prone himself and he comes at me I'd shoot again.
A 6'4″ 300lb guy is 4 inches taller and 200% of my weight. He's gonna fuck me up with his bare hands and that's just the way things go so yeah I'm gonna light him up.
And I'm gonna keep clicking until he stops.
That's my (clinical and detached) use of force assessment. It's solid. It's legal. I can put myself in that situation and know I'd probably do the same.
That said…
It's so easy to armchair quarterback, but I think it's important here.
We make split second decisions and they seem unimportant and harmless.
But in this case I seriously question Darren Wilson's tactics.
In the grand jury findings, Wilson claims he saw the pack of cigarillos in Brown's hand and it clicked that he might be the suspect. My problem is, if I see a guy I suspect was involved in a robbery and he's as big as Brown was and he's got a friend I identify and track. I don't confront.
I wait until odds are in my favor, and I only move when backup arrives.
I'm a county deputy and my backup is usually 15 minutes away if I even have it.
But Wilson was a city cop and his backup was coming quick.
The way I was trained was to never put yourself in a position to fight unless you know you're gonna win.
Sounds cowardly but we have to win. We just have to. It's not about pride our who's tougher. We gotta win.
I don't even think a taser would have made a difference.
This job comes down to pure fucking instinct most times and this was a clusterfuck neither brown nor Wilson anticipated.
It comes down to this.
Wilson didn't use effective tactics
If he had… I wouldn't be writing about it. The incident would have been one of the millions like it that occur in the United States every year that do not end in a fatality.
If Wilson would have been more patient and used better tactics Brown would still be alive and Wilson would still have a career. I don't think his actions rise to the level of criminality but I do find them negligent.
This is the first of what we hope are numerous future interviews with Officer Valentine. We would like to thank him for coming forward, and putting his job on the line to speak truth to power.
(Article by Barney Cox and Moreh B.D.K.; image by Counter Current News)


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Best Indie Paranormal Thrillers
Best Indie Paranormal Thrillers
Titles from J.M. DeBord and other indie authors who write quality paranormal-themed fiction.
Radical Owl created this book list on IndieBound.org because buying local and independent is important to your economy and your community.
Support independent bookstores by shopping at your local indies.
Print this list! RSS Feed
Published by: Eos
Edition: Mass Market Paperbound (ISBN #9780380729401)
Want to create your own book Wish List on IndieBound.org? It's fun and easy to do.
Learn more…


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This alternate history related article is a stub. You can help by expanding it. Alki is a city in the US state of Washington, situated around Alki Bay and on Puget Sound. The city is considered the northern anchor of the Alki-Tacoma-Olympia metropolitan area in western Washington, although the smaller city of Port Gardner is also often referred to as the northernmost terminus of the metropolitan area.
Alki is the second-largest city in Washington (after Tacoma) and is built along the confluence of the Duwamish River and Alki Bay. While the initial settlement was built on Alki Point itself, the city's financial district developed along the eastern shore of the bay in the early 1900's and Alki Point itself, typically referred to as West Alki, became an affluent suburb over time. The city has a population of about 225,000 as of the 2010 census, making it only slightly larger than Spokane in eastern Washington.
The city is known for its tough, gritty blue-collar image thanks to generations of railroad and dock workers and its rich history of labor unions. The city is bordered to the south by the city of Duwamish and to the north by Kingstown, which lies across Lake Union and Portage River from the city's northern neighborhoods. It is bordered to the east by Lake Washington, to the west by Puget Sound and Alki Bay, and to the north by Portage River and Lake Union. Alki is the seat of Washington County.


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Do you have a horrible habit of waking up in the middle of the night and end up lying awake for hours anxiously watching the clock, waiting for your alarm to go off? Insomnia is a common problem that takes its toll on your energy, mood, health and ability to function on a daily basis.
Well, it's time to stop losing sleep over insomnia and tackle it. Here are a number of sure fire ways to combat middle-of-the-night insomnia – let the zzzzz's begin!
1.Stick to regular sleep hours
It's vital that you go to bed and wake up at roughly the same time every day as this will programme your body to sleep much better at night time. In order to do this, you should choose a time when you're most likely to feel sleepy.
2. Create a restful sleeping environment
Your bedroom should be kept for rest, relaxation and sleep and reflect this in the way it's decorated and laid out. Purchase a blackout blind and a high quality bed to ensure you're as comfortable as possible while asleep, further preventing you from waking up during the night. By choosing a bed and a mattress that suits your back, you can look forward to maximum comfort throughout your dreams.
You should also ensure your room isn't too hot or too cold, possibly leave a window ajar throughout the night to let in a flow of fresh air.
3. Ditch the caffeine
Try to cut down the amount of caffeine and stimulants you have, especially during the evening. You could set yourself the four o'clock rule to prevent yourself from drinking tea or coffee after this time.
Caffeine is known for interfering with your sleeping pattern and preventing a deep sleep, so be sure to ditch this from your diet.
4. Exercise regularly
Taking part in moderate exercise on a regular basis, such as swimming or walking, can really help to relieve some of the tension you've built up over the day. However, make sure you don't do any vigorous exercise close to bedtime as the endorphins will keep you awake.
5. Don't eat too late
Too much food or alcohol before you go to bed is sure to disrupt your sleeping patterns. With this in mind, it's important not to eat past seven o'clock as this will allow plenty of time for your food to digest, giving you a peaceful nights sleep.
6. Relaxation is key
You should always try to relax before you go to sleep. You can do this by running yourself a hot bubble bath or laying on your bed listening to a little music – preferably something gentle and not trance!
7. Stop worrying in bed!
Your bed is where you sleep, not where you worry. If you have things on your mind, whether it's that big board meeting tomorrow or your child's first day at school, it's important to get it off your mind before you get between the sheets. If you can't sleep, don't lie there worrying about it, get up and do something you find relaxing until you feel tired.
Find plenty of hints and tips on how to get a good nights sleep and more in our inspiration and advice section.


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IMC Network
From the Open-Publishing Calendar
From the Open-Publishing Newswire
Indybay FeatureRelated Categories: Central Valley | Government & Elections
Dump Nunes!
This is a 10:30 minute video showing some of the candidates who are running against Devin Nunes in the 22nd Congressional District. The event was held at The Grange Meeting House in Visalia on Friday, June 16th, 2017.
Copy the code below to embed this movie into a web page:
Download Video (368.7mb)
Tulare County-based Progressives United for Social Justice and Human Rights (PUSH) hosted a candidate forum featuring several candidates who are vying for Congressman Devin Nunes' seat in the House of Representatives. The candidates attending this forum were:
Bobby Bliatout, Ricardo Franco, Andrew Janz, Dr. Mallory Kremer and Jose Sigala. Over 100 people attended this candidate forum.


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My birthday celebration started a week early, when I selected The Melting Pot for our date night! This is my favorite restaurant, I just LOVE fondue! We had a wine flight, a caprese salad (my favorite salad yet there), managers special and smores for dessert! YUM! Such a perfect night with my favorite man... and, I really need to find a copy cat recipe on Pinterest for their green goddess dip!
Then, on Friday these beauties arrived at our house. :) Brian even put ames soeurs on the card, which means soul mate in French! He also had that engraved on my wedding band. I haven't gotten sunflowers for awhile as flowers, they are beautiful!
On my actual birthday we went to lunch at Cracker Barrel with my family and I finally got to try their Coca-Cola cake. Then, we headed to the mall so Autumn could pick ME out a dress for my birthday. She insisted on this, so we went in F21 and she had the funnest little time looking at all of the dresses for me. Nathan got me some Lush lotion for me since I was almost out.
For dinner, we picked up Pi pizza on the way home and ate it while we watched Mary Poppins at home. The last time I had watched Mary Poppins, she was older then me and not the other way around! lol! The kids LOVED the movie, I was so glad! Such a perfect little family night!
And, there was an ice cream cake of course! This was the first ice cream cake I had ever had from Cold Stone, it was soooo good! I really love it when there is ice cream PLUS cake.
I am so blessed to have such a thoughtful, sweet and loving family to celebrate my birthday with!
Are you ready for the meal plan for this week?
Menu Plan for Week of August 10, 2014:
Sunday - Pi Pizzeria, Salad, Ice Cream Cake and Birthday Cake Martini's!!!
It's my birthday and I want to relax at home with some pizza in front of the TV with Brian and the kids. I love chilling with my family and nothing could be more perfect than that on my birthday. Don't worry, the martinis will be AFTER the kids are in bed... :)
Monday - Spaghetti Pie with Garlic Bread
Tuesday - Frozen Pizza
I know, pizza twice this week... but, we have a free pass to get into the library tonight from doing the reading program at the library. We won't have a lot of time for dinner before we need to get there, so I am going to do a quickie frozen pizza meal! We might even go and get Dunkin Donuts after we leave the Magic House around 8 p.m. It is sort of a library night at the Magic House tradition for us!! That place makes you hungry with all of the running around you do!
Wednesday - Nathan's Hot Dogs, Corn on the Cob and Watermelon
Autumn doesn't like hot dogs (these kids!!! what am I going to do with them?), but she loves corn and watermelon... so, I may make her up some chicken nuggets or something and the rest of us will enjoy our hot dogs. She LOVED hot dogs last summer, but now she says they 'tickle her throat'... um, yeah, whatever. ;)
Thursday - Crabby Patties with Crinkle FriesThese are really similar to White Castle hamburgers (or Krystal) and the kids love them! I will serve them with some fries hot out of the oven.
Friday - Skinny Zucchini Lasagna, recipe HERE
I just LOVE this recipe and it just makes sense to make it as often as possible before summer is over! It does take a little bit longer than I'd like to make, BUT it is so worth it... and, we'll have leftovers for lunch on Saturday! I am getting my hair done on Saturday (finally, only 4 weeks behind my usual every 8 weeks) and I won't have to worry about lunch once I get home... all I will have to do is warm this delicious recipe up in the microwave! YUM!
Saturday - Dinner Out
Not sure where, but we'll definitely be going out on Saturday evening!
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Mommy Monday Blog Hop


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Welcome to your 2nd Grade class at Lexington,
Let's get ready for an awesome 2016-2017 school year!
Hi, there! My name is Ms.McLaurin, I have been teaching 10+years This will mark my 6th year being at Lexington! I hold a Bachelor's degree in Elementary Education 1-8. I also have a Master's degree in Curriculum and Instruction.
Don't forget to use the links to the left of the screen!
As always, if you need me call me at Lexington 322-9753 ext 4495
or email me: firstname.lastname@example.com
Some Important Things to Remember
**Join the Remind App for important class announcements! **
Download the Remind App to your phone and then join
MsMcLaurin's ELA Class @mcela2
MsMcLaurin's Homeroom @aprilmc
READ, READ, READ every night, practice pays off!
Lexington Yellow folders & Assignment Books will go home and will need to be returned to school everyday. Please help your child remember to empty papers on the Keep at Home side.
Sign & Return papers will go home on Tuesdays.
Please sign the bottom of the conduct sheet and return the
Yellow Folder with papers included the next day.
On Mondays, I will put a new Classroom Newsletter and ELA Homework Worksheets in the Yellow folder.
Conduct sheets will be sent home each day and will need to stay in your child's Yellow Folder—Please review it & return to school the next day.
ELA and Spelling homework will be given on a worksheet. Students are to complete the day's section each night. Please turn in homework only on Fridays.
Spelling can be practiced daily or as often as you think your child should.
Homework is primarily used only for practice of the day's lesson and includes material that your child will be tested on for that week.
Please remember to review and study all information and pages sent home each day.