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Austin Leeds
I will mix, master, produce, co-produce, ghost write, and remix your productions. Ready for radio, festivals, & charts! I've been producing for 20 years & have tracks on Spinnin, Armada, Ultra, Toolroom. My music has been played by Tiesto, Armin, Guetta, Avicii, Umek, & more! I've collaborated with Avicii, Paul van Dyk, & Markus Schulz.
Nicolas Essig
Hi My name is Nico. I am a French sound engineer based out of Los Angeles. i graduated in 2008 from Berklee college of music's prestigious sound engineering and music production program. I ve have Been mixing, mastering and producing for over 10 years. Many of the projects have been nominated and won Grammys.
Eileen Jaime
Hello, I am a professional singer/songwriter from Miami, FL. I can write, record, and sing over your productions in any genre. I have worked with AVICII, Austin Leeds, Grammy Award Winning Mixer Jimmy Douglass, Starkillers, & many more artists. All work is WORK FOR HIRE - Just a one time fee. No messy percentages or royalties.
Luke Saunders
Producer/Mixing and Mastering Engineer One half of production duo Bordertown. Have done some high profile remixes including Yoko Ono, Avicii, Iggy Azalea and Rita Ora. Have done mixing and mastering for various indie labels and majors.
GI Studios
GI is a professional Mixing & Mastering Engineer who's worked with artists such as Charlie Puth, Tori Kelly, Tinashe, & Fifth Harmony. He has over 16 years experience in professional audio & has been personally mentored as a Mix Engineer by legend Dave Pensado (Beyonce, Justin Timberlake, Christina Aguilera) working as his mix assistant.
Neorone Production
We Are a Los Angeles Based Production Team. We have worked on Multiple Grammy Winning and Nominated Project.
Bionic Sound
The Workshop for the good sound! Especially for EDM! What we offer: > Vocals for your song > Mixing > Mastering > CD Authoring incl, DDP image creation > Post Production > Music and Audio Production
Bains/Crosby Collective Studio
Chief Engineer and founder Crosby Collective Recording Studios specializing in Mixing and recording.
John Pennington
I'm a Sound Engineer with 30 years of Experience in Live, Studio And Broadcast. I mix and record Live Shows and then mix that show in my studio for DVD presentations or TV I also Mix music for TV live Broadcasts. I use Protools and all current DAW. I have a studio in Baltimore. and a mobile recording Studio Facility.
Jp Did This 1
Im PKA Jp did this 1, as a producer / writer I have produced records for Machine Gun Kelly, The Game, Rick Ross, Avicii, David Guetta, Fergie, Prince Royce, Kid Rock, Jeezy, Gucci Mane...
Color Sound Studio
Founded by David Hachour and Florent Sabaton, ColorSound is a professional audio mixing and mastering studio based in Paris. We are one of the few mastering studios to offer real analog mastering services with a true analog summing. We offer stem, hard, and stereo mastering, both on-site and online.
Upside Down Entertainment
UpsideDown Entertainment is a full fledged music production house located in Boston, USA offering remote services to clients all over the world. We specialize in: - Music Production - Motion Picture Scores - Recording, Production and Development of Solo Artists & Bands - Remixing - Mixing & Mastering Services www.djupsidedown.com


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Question: Atlantic Ocean Kamp grounds Inc Atlantic borrowed 60 000 from
Atlantic Ocean Kamp grounds, Inc. (Atlantic) borrowed $ 60,000 from Camden National Bank (Camden National) and executed a note and mortgage on property located in Camden, Maine, securing that amount. Maine permits strict foreclosure. Atlantic defaulted on the loan, and Camden commenced strict foreclosure proceedings pursuant to state law. After the one year period of redemption, Camden National sold the property to a third party in an amount in excess of the mortgage and costs of the foreclosure proceeding. Atlantic sued to recover the surplus from Camden National. Who wins? Atlantic Ocean Kamp grounds, Inc. v. Camden National Bank, 473 A. 2d 884, 1984 Me. Lexis 666 (Supreme Judicial Court of Maine)
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NAGOYA — Just call him superman.
A day after capturing his 15th career title, Mongolian yokozuna Hakuho capped off a stellar performance with a superhuman feat against ozeki Baruto on Sunday, making him the only man in the history of sumo to win three consecutive meets with perfect 15-0 records.
With a victory over the Estonian man-mountain in a mouthwatering final match of the 15-day Nagoya Grand Sumo Tournament, Hakuho stretched his personal winning streak to 47 bouts.
In the finale, Hakuho held off a rampage from Baruto (8-7), who shook the yokozuna to and fro with his massive frame, in front of a riveted auditorium at Aichi Prefectural Gymnasium.
But never one to shrink from a challenge, Hakuho reached deep into his energy reserves before finding a way to dump the ozeki with one mighty left-handed overarm throw, causing fans to erupt with cheers and flying "zabuton" cushions around the auditorium.
No one was even close to Hakuho, who set the bar so high that his nearest challengers finished four losses off the pace.
"I'm so happy," said a teary-eyed Hakuho. "I'm thankful to all the fans who have rooted for me throughout the 15 days. This has been a tough basho (because of the gambling scandal), but I was able to focus myself in mind and body."
Hakuho also achieved a record that eluded Chiyonofuji by one win, despite the former yokozuna great's legendary 53-bout win streak. Hakuho is the only wrestler to have won three consecutive meets without a loss since the introduction of the six-tourney system in 1958.
"The Wolf," as Chiyonofuji was known by fans, ripped through opponents from the seventh day of the 1988 summer meet to the penultimate day of the Fukuoka meet in November the same year, but missed the record when he lost to Onokuni on the final day.
"This (record) was never something that I was aiming to achieve. I think I got this result because of my strong feelings about sumo and the everyday training I put in. I promise to give my all until the last day of the next basho as well," said Hakuho.
Before the start of the makuuchi bouts, acting Japan Sumo Association chief Hiroyoshi Murayama apologized once again in a statement to sumo fans over a gambling scandal rocking the sport and alleged ties to organized crime, with Hakuho and all of the "sanyaku," wrestlers in the top three ranks below yokozuna, bowing in a row.


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Let us know when your receive great advice so we can thank our lawyers and point others with similar questions in the right direction.Got it!
I have been separated from my husband for two years and am currently pregnant with my boyfriends baby . I understand that I can not get a divorce until the baby is born , but want to know if I will be able to give the baby my boyfriends last name and if he would be able to sign the birth Certificate .
You should check with the hospital where you will give birth. They may have specific policies in place that will mandate how the paternity will be cited on the birth certificate (BC).
Regardless of whose name is on the BC, your husband is the legal father of the child until the legal documents of your divorce state otherwise. You should engage a local attorney ASAP, to initiate your divorce. Although it cannot be finalized until the birth of your baby, you can get it started so that as soon as your child is born, you can finalize it. Then your boyfriend can file an acknowledgement of paternity. ( Your attorney may be able to combine these processes.)
Good luck!
This answer is general information which does not establish any attorney-client relationship between the person asking a question and the person answering, or a duty to respond to ongoing questions; nor is it intended to replace competent legal assistance in the jurisdiction where the matter/issue arises or is before a Court.Ask a similar question
I agree with the prior comment regarding the acknowledgement of paternity however, there must also be a denial of paternity by your husband who is the "presumed father." The following information is from the Texas Bureau of Vital Statistics: "If the parents are not married to each other and want to establish a legal father and/or add the father to the child's birth certificate, they may voluntarily complete the Acknowledgment of Paternity (AOP) form. The AOP must be signed by both parents before a witness or witnesses. If the mother is married to someone other than the biological father of the child or has been married within 300 days before the birth of the child, the AOP must also include a Denial of Paternity from her husband or former husband. The AOP must be filed with the Bureau of Vital Statistics (BVS). The AOP forms are supplied upon request by the Bureau of Vital Statistics. "
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Fresh Consumer Insights:<br />Food Label Dietary Info Crucial for First-time Purchases: FDA Study
With a larger percentage of U.S. consumers increasingly aware of the link between diet and heart disease, more than half are routinely reading food labels when purchasing products for the first time, according to key findings of the Food and Drug Administration's (FDA) latest Health and Diet Survey released earlier this week.
With a larger percentage of U.S. consumers increasingly aware of the link between diet and heart disease, more than half are routinely reading food labels when purchasing products for the first time, according to key findings of the Food and Drug Administration's (FDA) latest Health and Diet Survey released earlier this week.
The 10th such survey the FDA has fielded since 1982, this latest installment is based on a 2008 telephone survey of more than 2,500 adults in every state and the District of Columbia, and provides a reliable snapshot of the nation's dietary habits. (Previous surveys were conducted in 2002 and 2004.)
Among the key highlights of this year's newly released FDA Health and Diet survey:
—More U.S. consumers know of the relationship between diet and heart disease. Ninety-one percent are aware of the link, an 8 percent jump from 2002. In addition, 62 percent of consumers mentioned fats as a factor related to heart disease, compared with 53 percent in 2002
—Eighty-one percent of consumers know that certain foods or drinks may help prevent heart disease or heart attacks. This result showed no change from 2002
—While fruits and vegetables were most frequently linked with reducing heart disease, fewer people made this link in 2008 than in 2002
—Consumers' awareness that trans fats in the diet may raise the risk of heart disease nearly doubled in just four years, from 32 percent in 2004 to 62 percent
—Knowledge that omega-3 fatty acids may lower the risk of heart disease increased from 31 percent in 2004 to 52 percent in 200
—Knowledge that saturated fat may raise the risk of heart disease was stable: it was 74 percent in 2004 and 73 percent in 2008
With regard to food labels, more than half (54 percent) of consumers said they read a product's label the first time they buy the product. That's a 10 percent increase from 2002.
Among those who in 2008 reported they read the nutrition label the first time they buy a product:
—Two-thirds use the label "often" to check how high or low a food is in calories, and in substances such as salt, vitamins and fat
—Fifty-five percent "often" use the label to get a general idea of the food's nutritional content, while 46 percent "often" use the calorie information on the label. Thirty-four percent rarely or never use the calorie information
—Thirty-eight percent of consumers said they use nutrient content claims (such as "low fat," "high fiber" and "cholesterol-free") "often"; 34 percent answered "sometimes."
—When asked whether they refer to the label claim of "0 grams of trans fat," 31 percent said "often" and 36 percent said "sometimes
FDA's latest Health and Diet survey also found differing degrees of trust regarding claims found on food labels. For example, 41 percent of consumers believe that all or most claims such as "low fat," "high fiber" or "cholesterol-free" are accurate, while 56 percent believe that some or none of them are accurate.
Also, 64 percent of consumers reported seeing nutrition labeling on menus, napkins or place mats in restaurants. About half of these consumers use this information often or sometimes.
The survey also examined eating habits, key highlights of which found that 54 percent of consumers reported eating breakfast seven days a week, while 8 percent said they skip the meal every day. In contrast, 86 percent said they eat dinner seven days a week, while 1 percent said they always skip it.
Additional information about the survey can be found by visiting www.fda.gov/Food/LabelingNutrition/ucm202775.htm.


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Katy Perry and Kenny Chesney Headline NBC's Macy's 4th of July Fireworks Spectacular06/05/2012
New York, NY – June 5, 2012 – NBC celebrates the nation's 236th birthday extravaganza with an all-star line-up of performances, including chart-topping superstars Katy Perry and Kenny Chesney. The show will air on Wednesday, July 4th 9-10 p.m. ET, 8-9 p.m. CT.
"NBC is thrilled to have Katy Perry and Kenny Chesney join us in celebrating America's birthday," said Doug Vaughan, Senior Vice President of NBC Entertainment's Special Programs and Late Night, "These stars' powerful voices and stage presence will serve as the perfect complement to Macy's captivating visual artistry."
Katy Perry is a global superstar whose two albums have generated a string of number one hit singles and multi-platinum sales during the past four years. The special edition, "Teenage Dream: The Complete Confection," available now, features 3 new songs including "Part of Me," her 7th Hot 100 number one, and "Wide Awake," which she wrote for her forthcoming 3D film, "Katy Perry: Part Of Me," in theaters July 5th.
Kenny Chesney will release his 13th studio album, "Welcome to the Fishbowl" on June 19. With more than 30 million album sold and 23 number one singles, Chesney has been honored with numerous awards from the Academy of Country Music, Country Music Association, American Music Awards, Country Music Television, Billboard Music Awards, and People's Choice Awards. The 20-plus all stadium "Brothers of the Sun" Tour with Tim McGraw is the summer's biggest party and will run through August.
The Macy's 4th of July Fireworks, the nation's largest Fourth of July fireworks display, returns to the Hudson River to illuminate the sky over New York City with a glorious spectrum of colors, shapes and sparkling dazzlers. More than 40,000 firework shells will ignite the sky for the nation's 236th birthday party. "Ignite the Night," the theme of the 36th annual Macy's 4th of July Fireworks display, will fire more than 40,000 pyrotechnic shells during a 25-minute synchronized display.
"As the nation's premiere Independence Day pyrotechnic display, Macy's Fireworks ignite the sky with a jaw-dropping celebration each year," said Amy Kule, executive producer of Macy's 4th of July Fireworks. "With America's help we have put together a show like no other, filled with incredible high-flying effects, choreographed to a soaring, patriotic and exuberant score that will cap off a magnificent day of celebration for millions."
About Macy's 4th of July Fireworks : Macy's 4th of July Fireworks are the nation's largest 4th of July pyrotechnic display. Produced by Macy's and designed in conjunction with Pyro Spectaculars by Souza of Rialto, CA, Macy's Fireworks are a digitally synchronized display of firepower that is choreographed to a rousing patriotic score. Annually fired from various locations across New York City, Macy's Fireworks feature shells from all over the world. Seen by more than 3 million spectators and millions of viewers nationwide, Macy's 4th of July Fireworks have become the nation's premiere 4th of July celebration event.
The telecast is a production of Universal Television in association with Macy's. It is produced by Brad Lachman Productions with Lachman serving as executive producer. Bill Bracken is producer. Lachman's credits include a variety of NBC specials, including the "Macy's Thanksgiving Day Parade," "Christmas in Rockefeller Center," in addition to "Don't Forget the Lyrics!" and a variety of other projects.
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text: New user here. I searched before posting but couldn't find any threads similar to my case. I don't work for Verizon but have an employee concession line. Got a free Moto Droid and such. I'm wanting to root my phone but the phone isn't mine and don't want to suffer any consequences. I will eventually have to send the phone back after I change positions or get an upgraded phone as they don't like me to have an outdated phone. I've done quite a bit of reading and it seems that if I root it I am able to run the sbf "update" and unroot completely then reactivate to get it back to a factory state. I suppose I have two main concerns. 1.) Is there any way after a full unrooting that they will be able to tell that it was ever rooted upon receiving the phone? 2.) Will it tip Verizon off that an employee line is repeatedly reactivating while I'm screwing around with rooting and unrooting? I believe I have to speak to someone every time since it's an employee line. Ultimately, are there any ways of getting caught in the end? =D Thanks in advance for any help.


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Estradiol-17β and its metabolites which are sequentially synthesized by cytochrome P450s (CYP450s) and catechol-O-methyltransferase (COMT) to form 2 and 4-Hydroxyestradiol (2-OHE2 and 4-OHE2) and 2- and 4-Methoxestradiol (2-ME2, and 4-ME2) are elevated during pregnancy. We investigated whether CYP450s and COMT are expressed in uterine artery endothelial cells (UAECs) and if E2β and its metabolites modulate cell proliferation via ER-α and/or ER-β and play roles in physiologic uterine angiogenesis during pregnancy. Cultured ovine UAECs from pregnant (P-UAECs) and nonpregnant (NP-UAECs) ewes were treated with 0.1-100 nmol/L of E2β, 2-OHE2, 4-OHE2, 2-ME2, and 4-ME2. ER-α or ER-β specificity was tested using ICI 182,780, ER-α-specific MPP, ER-β –specific PHTPP antagonists and their respective agonists ER-α-specific PPT and ER-β –specific DPN. Angiogenesis was evaluated using BrdU Proliferation Assay. Utilizing confocal microscopy and Western analyses to determine enzyme location and levels, we observed CYP1A1, CYP1A2, CYP1B1, CYP3A4 and COMT expression in UAECs; however, expressions were similar between NP-UAECs and P-UAECs. E2β, 2-OHE2, 4-OHE2, and 4-ME2 treatments concentration-dependently stimulated proliferation in P-UAECs, but not NP-UAECs; 2-ME2 did not stimulate proliferation in either cell type. Proliferative responses of P-UAECs to E2β were solely mediated by ER-β, whereas responses to E2β metabolites were neither ER-α nor ER-β mediated. We demonstrate an important vascular role for E2β, its CYP450- and COMT-derived metabolites and ER-β in uterine angiogenesis regulation during pregnancy that may be dysfunctional in preeclampsia and other cardiovascular disorders.
Pregnancy is associated with dramatic uterine blood flow (UBF) rises resulting from vascular adaptations including vasodilatation and angiogenesis.1 These adaptations are critical in pregnancy since their dysfunctions are implicated in pathologic pregnancies such as preeclampsia which complicate 6-8% of all pregnancies in the USA and account for 50,000 maternal deaths per year worldwide.2,3,4
Regulation of vascular adaptations during pregnancy is mediated partly by estrogens, which are elevated during gestation.5 Estradiol-17β (E2β) infusion in sheep markedly reduces uterine and systemic vascular resistance causing rises in uterine and systemic blood flows.6 Uterine arterial administration of the nonselective estrogen receptor (ER) antagonist ICI 182,780 in pregnant sheep lowers UBF, demonstrating that endogenous estrogen via ERs helps maintain uterine perfusion.7 In human umbilical vein endothelial cells (HUVECs) 8 and myometrial microvascular ECs, E2β promotes proliferation, an index of angiogenesis.9
The effects of estrogen on uterine vascular adaptations may be further modulated by its biologically active metabolites. E2β metabolism catalyzed by cytochrome P450s (CYP450s) and catechol-O-methyltransferase (COMT) produces the catecholestradiols 2-Hydroxyestradiol (2-OHE2) and 4-Hydroxyestradiol (4-OHE2), and the methoxyestradiols 2-Methoxestradiol (2-ME2) and 4-Methoxyestradiol (4-ME2).10,11 Evidence supports the involvement of E2β-derived metabolites in pregnancy and in the regulation of angiogenesis; 2-ME2- and COMT-deficient mice exhibit preeclampsia-like symptoms including impaired angiogenesis and hypertension.12 Treatment with low concentration of 2-OHE2, 4-OHE2, 2-ME2, or 4-ME2 induces proliferation in cultured HUVECs whereas high concentration of 2-OHE2 or 2-ME2 inhibits proliferation.13
Thus, we tested the hypothesis that CYP450s and COMT may be expressed in the uterine vasculature and that E2β, its CYP450s and COMT-derived metabolites participate in the regulation of uterine angiogenesis during pregnancy. Late pregnant and nonpregnant ovine uterine artery endothelial cells (P-UAECs and NP-UAECs) consistently express ER-α and ER-β and exhibit pregnancy-specific responses to angiogenic ligands demonstrating that they are a good model to evaluate direct receptor-mediated actions of E2β and its metabolites.14,15 We investigated: 1) the expression and intracellular distribution of CYP450s and COMT in P-UAECs versus NP-UAECs; 2) whether E2β, 2-OHE2, 4-OHE2, 2-ME2, and 4-ME2 stimulate greater proliferation of P-UAECs than NP-UAECs; and 3) if E2β and its metabolites induce proliferative responses via ER-α and/or ER-β.
For complete details on specific materials and methodology, please see http://hyper.ahajournals.org.
Cell preparations were approved by the University of Wisconsin-Madison School of Medicine Research Animal Care Committee as previously described.14,15 UAECs were isolated and validated from late gestation (120-130 days; term= 147 days; n=6) and nonpregnant (luteal n=5 and follicular n=2) ewes.15 At passage 5, ~ 70% confluence, cells were transferred to slides, 96 well plates, or lysed for protein extraction as needed for respective experiments.
Western immunoblotting was performed as previously described.14 CYP1A1, CYP1A2, CYP1B1, CYP3A4, COMT, and ER-β expressions were detected using mouse anti-CYP1A1and rabbit anti-CYP1A2, anti-CYP1B1, anti-CYP3A4, anti-COMT or anti-ER-β antibodies. GAPDH was utilized as a loading control.
Immunofluorescence confocal microscopy was performed as previous described.14 UAECs were washed twice with ice cold PBS and fixed for 15 min with 3% paraformaldehyde. Fixed cells were rinsed with 50 mM glycine solution, permeabilized with 0.1% Triton-X for 3 min, blocked for 30 min with goat serum and incubated (20 min) with primary antibodies for CYP1A1, CYP1A2, CYP1B1, CYP3A4 and COMT. Subsequently, cells were incubated (30 min) with secondary antibodies Alexa Fluor 488 anti-mouse or anti-rabbit IgGs. Scanning was done with a radiance 2100 MP Rainbow confocal/multiphoton laser scan microscope system (Bio-Rad, Hercules, CA).
UAEC proliferation experiments were performed in quadruplicates and replicated in at least six NP-UAEC and P-UAEC preparations. For concentration response studies, UAECs in 96 well plates were serum starved (24 hrs) in endothelial basal medium (EBM) and medium was replaced with EBM or EBM containing 0.1, 1, 10 or 100 nmol/L E2β, 2-OHE2, 4-OHE2, 2-ME2 and 4-ME2 (24 hrs). ERs were blocked by pretreating UAECs for 1 hr with 1μmol/L of the ER antagonist ICI 182, 780 (ICI), or ER-α selective antagonist 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinyleth oxy)phenol]-1H-pyrazole dihydrochloride (MPP), or ER-β selective antagonist 4-[2-Phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrim idin-3-yl]phenol (PHTPP). Additional concentration response studies were performed using 0, 0.1, 1, 10 or 100 nmol/L of the ER-α selective agonist 4,4′,4″-(4-Propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT) or the ER-β selective agonist 2,3-bis(4-Hydroxyphenyl)-propionitrile (DPN). We also studied the effects of 0.1 nmol/L PPT + 0.1 nmol/L DPN and 1 μmol/L PHTPP + 0.1 nmol/L DPN to further evaluate receptor activation, additive effects, and specificity of ER-β selective agonist receptor activation.
BrdU was added for 16 hrs during the 24 hrs of steroid treatment and an in vitro index of proliferation was evaluated. Plates were read using Synergy HT Multi-Mode Microplate Reader (BioTek, Winooski, VT). Results are expressed as fold increases over untreated control after subtracting the "blank" (wells incubated without Brdu).
Data (means ± SEM) were analyzed using a Two-way ANOVA with "Pregnancy" and "Concentration" as two "between" factors. Analyses of simple effects were performed using One-way ANOVA followed by post-hoc Student-Newman Keuls test. Pairwise comparisons were performed using Bonferroni or Student-Newman-Keuls test. Biphasic concentration response (deviation from the standard monotonic sigmoid shape) description was determined by nonlinear regression using the logarithm of agonist concentration against various responses. Level of significance was established a priori at P<0.05.
Western analyses indicated the presence of CYP1A1, CYP1A2, CYP1B1, CYP3A4, and COMT in NP-UAECs and P-UAECs (Figure 1A). However, no differences were seen between NP-UAECs and P-UAECs in their levels of expression (Figure 1B). Confocal microscopy revealed no difference between NP-UAECs and P-UAECs in intracellular distribution patterns of these enzymes. Therefore, unless noted, P-UAECs images are shown. CYP1A1, CYP1A2, and CYP3A4 were localized in cytoplasmic and nuclear compartments of P-UAECs (Figure 2A, B and D). CYP1B1 was localized in the nuclear region, whereas COMT was localized in the cytoplasmic compartment (Figure 2C, 2E).
Biphasic concentration proliferative responses were observed in P-UAECs after E2β treatment with maximum responses observed at a concentration of 0.1 nmol/L (Figure 3A). In contrast, E2β did not induce NP-UAEC proliferation at any concentration. Similarly, P-UAECs but not NP-UAECs exhibited a biphasic proliferative response to 2-OHE2 and 4-OHE2 (Figure 3B, 3C). The magnitude of P-UAECs proliferation at 0.1 nmol/L of E2β, 2-OHE2 and 4-OHE2 were 2.07 ± 0.16, 1.79 ± 0.02 and 1.78 ± 0.02 fold of control, respectively.
2-ME2 did not stimulate proliferation of P-UAECs or NP-UAECs (Figure 3D). 4-ME2 at all concentrations induced proliferation in P-UAECs, but not in NP-UAECs (Figure 3E). Proliferation of P-UAECs at the physiologic concentration of 0.1 nmol/L of 4-ME2 was 1.50 ± 0.16 fold of control (Figure 3E). However, response to 4-ME2 was not biphasic and the maximum proliferation of 1.74 ± 0.04 fold was observed at 100 nmol/L. Additional validation of cell proliferation utilizing ViaLight Plus Kit (Lonza Inc., Rockland, ME) was performed and it confirmed increases in total viable cell numbers after treatment with E2β or its metabolites.
Antagonism with ICI was tested at a physiologic concentration of 0.1 nmol/L E2β and its metabolites (Figure 4). ICI alone had no effect on P-UAEC proliferation, however, it totally abrogated proliferative responses to E2β indicating the requirement of ER-α and/or ER-β. In contrast, ICI did not have an effect of the proliferative responses of P-UAECs to 2-OHE2, 4-OHE2 and 4-ME2. Figure 4 also illustrates that at 0.1 nmol/L, E2β was more potent than 2-OHE2, 4-OHE2 and 4-ME2 which were equipotent in stimulating P-UAECs proliferation.
In P-UAECs, ER-α blockade with 1 μmol/L MPP did not abolish the proliferative effects of E2β, 2-OHE2, 4-OHE2, or 4-ME2 (Figure 5). In contrast, E2β-induced proliferation was completely inhibited by 1 μmol/L of the ER-β selective antagonist PHTPP (Figure 6). However, PHTPP did not alter P-UAECs proliferative responses to the estrogen metabolites.
We further evaluated if the ER-β-mediated proliferative responses in P-UAECs were due to an increase in ER-β protein levels between NP-UAECs, P-UAECs and P-UAECs treated with 0.1 nmol/L E2β or its metabolites. Shown in Figure S1 (please see http://hyper.ahajournals.org), ER-β expressions were not different amongst these groups (P=0.943).
Treatment of P-UAECs with ER-α selective agonist PPT did not induce proliferation (Figure 7A). In contrast, all ER-β selective agonist DPN concentrations stimulated cell proliferation 1.50 ± 0.05 fold of control (Figure 7B). Because these P-UAEC responses were less than E2β alone and did not exhibit a concentration-dependent response, we examined combination of PPT (0.1 nmol/L) and DPN (0.1 nmol/L) (Figure 7C). No further increases in P-UAEC proliferative responses were observed. Moreover, pretreatment with 1 μmol/L PHTPP completely inhibited DPN-induced responses in P-UAECs; (Figure 7C).
The key novel findings observed from this study are: 1) UAECs express CYP1A1, CYP1A2, CYP1B1, CYP3A4, and COMT; 2) E2β, 2-OHE2, 4-OHE2, and 4-ME2 stimulate P-UAEC, but not NP-UAEC, proliferation; and 3) E2β-induced cell proliferative responses are mediated primarily via ER-β, whereas E2β metabolites-induced proliferative responses are independent of ER-α and ER-β.
UAECs constitutively express enzymes that may metabolize E2β to its hydroxy-(CYP1A1, CYP1A2, CYP3A4, CYP1B1) and subsequently methoxy- (COMT) metabolites. Consistent with our findings, are reports showing that CYP450s and COMT are expressed in aortic, coronary artery and umbilical vein ECs.11, 16, 17,18 However, this is the first characterization of the localized intracellular expression of CYP450s in endothelial cells. Our data also confirm findings that COMT is primarily an intracellular cytosolic enzyme.19 Although little is known about the intracellular localization of these enzymes in endothelial cells, it is possible that intracellular compartmentalization is associated with enzymatic function.
The physiologic plasma E2β concentration in women ranges from 0.1-2.2 nmol/L and increase dramatically during pregnancy.5 We demonstrate that a physiologic concentration of E2β stimulates P-UAEC, but not NP-UAEC proliferation. The P-UAEC proliferative responses are similar to estrogenic stimulation of HUVECs and retinal microvascular ECs.8,13,20 Moreover, E2β promotes murine endometrial endothelial proliferation in vivo.21,22 These current data are also consistent with our previous findings23 that E2β increases P-UAEC [H3]-thymidine incorporation and tube formation; however maximum P-UAEC responses to E2β were seen at 1 nmol/L23 and not 0.1 nmol/L; NP-UAECs were not evaluated. These results demonstrate that P-UAEC proliferative responses are induced by gestational programming at the level of endothelial cell signaling, supporting reports that pregnancy-induced programming in P-UAECs leads to increased responsiveness to agonists and these effects are retained in cultured primary cell lines.15 Furthermore, the complete lack of mitogenic response of NP-UAECs may be specific to E2β since NP-UAECs show proliferation in response to ATP,VEGF, bFGF, and high (≥5%) serum.15, 24, 25,26 The mechanistic significance of pregnancy-induced estrogenic programming on physiologic angiogenesis in P-UAECs remains to be elucidated.
Plasma catecholestrogens levels in pregnancy are 10-fold greater than in nonpregnant women.27 Our finding that low levels of 2-OHE2 and 4-OHE2 stimulate P-UAEC, but not NP-UAEC proliferation, supports the proposal that CYP450s- and COMT-derived metabolites of E2β may play roles in the regulation of uterine angiogenesis during pregnancy. Low concentrations of 2-OHE2 and 4-OHE2 stimulate HUVEC proliferation and direct uterine arterial infusion of 2-OHE2 in nonpregnant sheep causes vasodilatation, whereas 4-OHE2 interacts directly with calcium channels to locally increase blood flow in gilts.13,28,29 These findings suggest that catecholestradiols play roles in pregnancy-induced vascular adaptations.
O-methylation of catecholestradiols produces less potent and antiproliferative metabolites of E2β.10 Interestingly, we demonstrate that 4-ME2 stimulated P-UAEC, but not NP-UAEC proliferation, consistent with observations that low 4-ME2concentrations stimulate HUVEC proliferation.13 However, 2-ME2 was not mitogenic on UAECs supporting numerous reports of its antiproliferative effects.30,31,32 The reason for divergent proliferative patterns between 2-ME2 and 4-ME2 is unclear. However, 2-ME2 disrupts tubulin polymeration and induces cell-cycle arrest in the mitotic phase in endothelial and smooth muscle cells.33,34,35 Thus, differences in association of 2-ME2 and 4-ME2 with regulators of mitosis, may likely account for their divergent responses.
Demonstrating a role for ER-α and ER-β, ICI abrogated E2β-induced P-UAEC proliferation, supporting previous observations that ICI blocks E2β-induced P-UAEC [H3]-thymidine incorporation23 and E2β-induced VEGF-mediated proliferation.9 Antagonism of ER-β with PHTPP abrogated E2β-induced P-UAEC proliferation and ER-β activation with DPN-induced proliferation demonstrating an ER-β only effect. However, although activation of ER-α with PPT did not alter P-UAEC proliferation, PPT stimulates proliferation of human myometrial microvascular endothelial cells.36 Therefore, the differences in P-UAECs proliferation in response to DPN, PPT, or E2β may be due to their distinct differences in affinity for ERs in association with the complex nature of ER-ligand complexes.37,38 Nevertheless, PHTPP inhibition of E2β- and DPN-induced P-UAEC proliferation validates that these E2β effects are solely ER-β mediated and independent of ER-α. Equally importantly, NP-UAECs, P-UAECs, and P-UAECs treated with E2β express similar levels of ER-β (Figure S1, please see http://hyper.ahajournals.org.) demonstrating that the ER-β-mediated E2β effects are not dependent on ER-β expression levels, but rather on other gestational-programming factors at the level of P-UAECs signaling.
E2β metabolites possess little affinity for classical ERs10,39 and unlike E2β, the effects of its metabolites on P-UAECs proliferation are not mediated via ER-α or ER-β. These results also indirectly suggest that CYP450s and COMT expressed in the UAECs do not possess high enough enzymatic activity under these conditions to metabolize E2β. However, E2β metabolites may induce proliferative effects via other estrogen associated receptors like GPR30 found in endothelial cells.40,41 Nonetheless, the exact mechanism of action of estrogen metabolites on uterine vascular ECs remains to be determined.
It is well established that E2β and its metabolites possess vascular protective effects on the cardiovascular system,42,43,44 but, little is known about estrogen metabolism and regulation of uterine angiogenesis during pregnancy. Therefore, understanding the biochemistry of E2β metabolism and the vascular physiology of E2β and its metabolites on the uterine endothelium may provide clues for understanding normal pregnancy-associated vascular adaptations and the dysfunction of endothelia in the pathophysiology of preeclampsia and other cardiovascular disorders.3,4
Terrance Phernetton, Jason Austin, Kreg Grindle, Gladys Lopez, Natasha Sean Fling, Kai Wang and Cindy Goss
Funding Sources Support: NIH Grants HL49210, HD38843, HL87144 (RRM), HL64703 (JZ), R25GM083252 (ML Carnes).
Disclosures None
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Top 10 Reasons Why Big Hero 6 Is Better Than Frozen
The Contenders: Page 3
There are fangirls, but they aren't nearly as annoying. It's not like Frozen, where you say one bad thing about it, you are attacked. I am talking about that one guest in particular... - AnnaOfArendelle332
There are some Big Hero 6 fan girls, but they are all tomboys or regular.
There is fan girls but they are not annoying like frozen fan girls who call us intagrets and write in caps
These fangirls are the reason why Hotel Transylvania franchise is THIS well-received for being very one-dimensionsional.V 2 Comments
And every time I see hans and his sideburns I want to throw up
Comparison showtime:
Only 4 characters are good looking: Elsa, Anna, Hans and Kristoff.
Side characters are too fat, too thin, too short, or their noses are too long and their faces look like a squashed pumpkin.
Big Hero 6:
The main characters are all goodlooking, yeah even a midget just looks gorgeous. All side characters are all good-looking and realistic so look closely, frozen retards!
Elsa, Anna, Hans & Kristoff all look ugly and all Big Hero 6 characters are all good looking, even Honey Lemon & Robert Callighan.
Frozen sucksV 1 Comment
It teaches us the meaning of perserverance and friendship. WHY ARE BIG HERO 6 HATE COMMENTS GETTING THUMBS UP?!
If my movie was real then women would be seen in a skirt or jeans, I even sometimes wear normal things and I am a Queen! - QueenIrisIceQueen
Even though I hated the movie, that is an accurate fact for the timesetting of Frozen.
Ya see, this is what I'm talking bout. Getting angry at people for saying girls aren't always in dresses. - Gamecubesarecool193
Anna and Elsa were fantastic sisters
You right! Anna and Elsa are still fantastic sisters. Anna and Elsa are the best sisters ever. It's always Jennifer Lee's fault for shattering Anna and Elsa's sisterly friendship and relationship bond. I really hate Jennifer Lee for doing that. I would never ever even hate Jennifer Lee if she hadn't shattered Anna and Elsa's sisterly friendship and relationship bond, Anna and Elsa could've remain inseparable sister best friends forever if it weren't for stupid Jennifer Lee's stupid decision for creating accidents by shattering Anna and Elsa's sisterly friendship relationship bond. Anna and Elsa's sisterly friendship and relationship bond is supposed to be way too strong and too hard to be shattered. Stupid Jennifer Lee shattering Anna and Elsa's sisterly friendship/relationship bond.
Why do you even care which set of orphaned siblings are better anyway? Huh? Who cares which set of orphaned siblings are better anyway? Huh? Anna and Elsa and Hiro and Tadashi Hamada are the best orphaned siblings in their own different ways so I don't even care which set of orphaned siblings are better anyway.
Hiro & Tadashi are like Mario & Luigi - brothers that teach other decent meanings and are thoughtful toward each other.
Anna & Elsa are more like Princess Peach and Princess Daisy, two "best friends/sisterly cousins" who quarrel a lot and never love each other.
I like how close they were. It's sweet - AnonymousChick
Tell me how does Hiro Hamada remind you of Mario and Tadashi Hamada remind you of Luigi. Huh? I really deserve to know do Hiro and Tadashi Hamada remind you of Mario and Luigi. You know the similarities between Hiro and Tadashi Hamada and Mario and Luigi, right? Please list the similarities between Hiro and Tadashi Hamada and Mario and Luigi so I'll know how.
Ugh just shut up about Jennifer Pee!V 6 Comments
Superhero movies rock!
Princess movies suck, especially Tangled.
Big hero 6 and The Incredibles were meant to be better than Frozen anyway...
I would watch any animated super hero movie
YEAH! WHOOOHOOO!V 1 Comment
Frozen fans, go back to the cloning factory you came from.
See you say you don't care what haters think even though you constantly diss them. Bravo!
Oh godŌĆ"
For Mochi's sake, Frozen fans, can't you just take your finger off the CapsLock key? You're starting to give me hypertension dammit!
Olaf didn't even played drums in Frozen, so how can you know Baymax plays the drums better than Olaf?
Because he CAN!
I think the Big Hero 6 universe is better.
I never ever even care which universe is better.
Yes! I wish Fransokyo was a real place, looks awesome - FrozenisOverrated
Fransokyo and Arendelle may be different but they're both awesome places.
You mean over 9000 times beter
San Fransoskyo is actually San Francisco :D.
Arebdelle is more like bitchesland
You mean Frozen contains all cowardly males?
Frozen haters and Tangled haters: You mean both all cowardly males and cowardly females contained by Frozen and Tangled, right?V 1 Comment
┬Ć"and hot bod of Tadashi butŌĆ"no. Hiro owns everything! Because he's hot, cute, adorkable, awkward, beau--(gets slapped by random fangirl: No! He's MINE! )
No. Back away fangals! HE'S MINE!
Gogo actually has a realistic but beautiful appereance
Anna's annoying, stupid, and she fell for Hans. Elsa is whiny and didn't care about her people when she froze her kingdom.
Elsa is stupid. It took her nearly killing her own sister to realize that being different was okay. - yuki-blue
Anna and Elsa are very as good as Hiro and Tadashi Hamada.
It kind of baffles me as to why anna was saved in the end but they killed off tadashi possibly I've stuff about how tadashi is still alive but he will turned into sunfire
The more I read of you the more idiotic you seem. Just because they aren't adopted doesn't make them any worse. Tadashi and Hiro are better because they have each other's back. Elsa never seemed to have Anna's back. And so what you think they are better? Everyone has their opinions. Now please shut up, you're doing nothing but making people hate them even more. (Ignore my username). - AnnaOfArendelle332
Anna and Elsa haters: Yeah I mean Anna and Elsa are stupid losers.
You mean, "are stupid losers".
Anna and Elsa rule!
Damn. Frozen Fans treat Anna and Elsa like real people. Ever heard of opinions because we can't hurt fictional characters' feelings. Plus did coincidentally most of your caps lock keys break because seems like it.
Your wrong their extremely stupid marrying someone on the first day you meet them really stupid
Anna haters: Anna's loneliness make no sense.
*Haldora doesn't like anyone insulting her sisters Anna and Elsa in front of her, if they do they end up either getting a beating from her or run away when they see her crack her knuckles.V 1 Comment
Recommended Lists
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List StatsUpdated 19 Aug 2017
73 listings
2 years, 250 days old
Top Remixes (5)
2. Hiro didn't go running off like a baby like Elsa
3. The effects
2. No princesses singing retarded songs
3. Haaairrry Baby hairy babyyy.....
2. Underrated
3. No princesses singing retarded songs
View All 5


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The officials were booked yesterday under the provisions of Prevention of Atrocities Act against Scheduled Caste and Scheduled Tribes, police said today.
Deputy Inspector General of Prisons (Eastern Region), Shashikant Shinde, who was promoted in 2010 to the present post, had filed a complaint with Dhantoli Police in Nagpur recently against the officers, including Principal Home Secretary Kailash Bilonkar, the then Additional Chief Secretary (Home) Umesh Sarangi and Mantralaya official R M Deshmukh.
As police did not initiate any action, he moved the court of Judicial Magistrate First Class (JMFC) presided over by A H Kashikar who asked the cops to register offences against the DGP and other respondents after ordering a probe.
The complaint was filed under the provisions of Prevention of Atrocities Act as the complainant belongs to Dalit community and has made allegations that his promotion was delayed because he comes from the backward class.
Hearing his complaint, JMFC, yesterday, directed Dhantoli police to register offences as alleged in the complaint.
Shinde alleged that though he was eligible for the promotion much before he got it but the Departmental Promotion Committee had overlooked his claim and delayed it.
He further alleged that his career was harmed by false reports submitted by his seniors.


text:
Aiken County police are searching for a suspect in a Tuesday afternoon shooting in Nicholson Village.
Police were called to Pine Street near Main Drive about 3:40 p.m. and found the unidentified victim with a gunshot wound to the hip. He told police he was in an argument with Dominique Roscelo Johnson, 24, and was attempting to walk away when Johnson shot him. Johnson was last seen leaving the area in a tan Cadillac.
The victim is in stable condition at Georgia Regents Medical Center.
Johnson will face charges of attempted murder, possession of a firearm during the commission of a violent crime and unlawful carrying of a weapon.
Anyone with information on Johnson is asked to contact the Aiken County Sheriff's Office at (803) 648-6811 or Crime Stoppers at (888) CRIME-SC.


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Born in 1961 - United States
Huston Ripley was born in the United States in 1961. For many years -- mostly in cafés, bars and restaurants -- he drew on napkins, which were readily available and provided the tactile surface he liked. Over time the drawings have continued to evolve through to his current work.
He has developed a personal idiom depicting such themes as fertility, growth and death that suggests various iconographies and conveys the spirit or idea of transcendence.
Creating these works has enabled him to express symbolically Eros, terror, peace, and other aspects of humanity by using universal shapes and structures revealed through the meditative process of drawing.
Huston Ripley Drawings, Adam Baumgold Gallery, New York City (USA)
Mezzanine: Huston Ripley, Feature Inc, New York City (USA)
Narcissus in the Studio: Artist Portraits and Self-Portraits, Pennsylvania (USA)
Academy of the Fine Arts, Philadelphia (USA)
Same Difference - Pennsylvania Academy of the Fine Arts, Philadelphia (USA)
About Face, Adam Baumgold Gallery, New York City (USA)
Surreal Naiveté: The Eye of an Emerging Collector, Miyako Yoshinaga Art Prospects, New York City (USA)
Sexy Time, A Group Effort, Morgan Lehman Gallery, New York City (USA)
"Drawn Out and Shot", Projects Gallery, Philadelphia (USA)
Summer Selections I, Fleisher/Ollman Gallery, Philadelphia (USA)
Description Contemporary Erotic Drawing, The Aldrich Contemporary Art Museum, Ridgefield (USA)
Itsy Bitsy Spider - Feature Inc, New York City (USA)
Innocence & Transgression, The Proposition, New York City (USA)
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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Form Cover Image position error on FirefoxAsked by AtTheCorner on September 21, 2015 at 09:23 AM
With Firefox, the position of the image "At The Corner" (the green/blue image at the top right of the page) is not correct. It should be in the middle of the page. How can I improve that ?
The problem comes from one of the CSS rules on your form:
.form-all:before {
content: "";
background-image: url("//www.jotform.com/uploads/AtTheCorner/form_files/smalllogo.png");
display: inline-block;
height: 30px;
position: absolute;
background-size: 199px 30px;
background-repeat: no-repeat;
width: 100%;
In order to fix it, all we need to do is to inject a CSS rule to override it:
.form-all:before { display: block !important; }
You can inject this code by opening your form in the Designer (Setup & Embed > Designer), and pasting the code at the bottom of the textbox under the CSS tab. Please refer to the images below for visual guide:
Please let us know how it goes.
Perfect. Thank you :)
JotForm Support
Hello AtTheCorner,
On behalf of my colleague, you are welcome.
Do get back to us if you have any questions.
Thank you!


text:
Never Quite The Same Vest
- Description
- Reviews
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- Product Vendors
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Product Description
This beautiful olive green vest is sure to make your fall wardrobe shine! Featuring a beautiful olive colored material paired with lightweight and soft material, it's a wonderful combination that will keep you stylish and comfortable all season long! It also features pockets for storing your essentials on each side, a drawstring at the waist and bottom hemline, snap closures all the way down that cover an additional zipper, and a comfy drawstring hood that is detachable! This is simply perfect for your adventures all season long - from watching a bonfire to enjoying pumpkin spice treats! Wear our Say That You Love Me Striped Blouse, our Caroline Distressed Machine Jeans, and our Bethany Booties for a fantastic fall look!
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My cousin is convinced he is ugly. You agree. 1-10
My cousin is convinced he is ugly. You agree. 1-10
Have an opinion?
I think your cousin shouldn't think of himself that way. I think he's a handsome man and he looks rather dashing in that suit. :)
I think a boost in his self confidence will help, and in all honesty that style suits him very well (pun partially intended :3). He looks sophisticated, professional, elegant, classy, and handsome :)
He's handsome :))
Nah, he looks pretty dapper... nice skin, nice smile :)
You cannot undo this action. The opinion owner is going to be notified and earn 7 XPER points.


text:
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Wie schützt man Kinder vor den gefährlichsten Apps?
Der weite Zugang von Internet zu jedem Benutzer hat zu verschiedenen Nachwirkungen geführt, und die Pay-offs überwältigen jeden Internetbenutzer gleichermaßen. Die Teenager und Kinder sind nicht viel intim mit Technologie und gefangen durch die whiz. Eltern bringen ihre Kinder alles Wesentliche und vergessen, dass sie sie in falschen Dimensionen verwenden können. Social Media ist ein Schlafzimmer für alle Süchtigen geworden; Verbringen sie 18-20 Stunden ihres Tages auf verschiedenen Anwendungen wie Facebook, Twitter, Whatsapp, Skye und Hangouts, Dating-und Hook-ups Apps und andere Freizeit-Websites. Der wichtigste Faktor zu berücksichtigen ist, ob mit diesen Anwendungen Ihre Kinder sind geschützt und sicher?
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Trotz einiger natürlicher Praktiken können in Aktion gebracht werden, um Social Media-Nutzung abzubrechen, aber bald nach einiger Zeit werden die Kinder wieder auf ihren vorherigen Tracks. Eltern können ihnen raten, nicht für eine bestimmte Aktion zu gehen. Kinder können vorgeschlagen werden, um die Nutzung des Internets zu minimieren, aber es gibt keine Garantie für Ergebnisse. Der einzige machbare und empfohlene Weg ist, sie zu überwachen und fangen red-handed, wenn sie tun, was ihnen gesagt wurde, bleiben weg von. Um frei zu sein von diesen bösen Dingen, sollten die Eltern erhalten und kaufen die überwachung-Anwendungen. Alle Smartphones wie iOS und Android unterstützen
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You can without much of a stretch incorporate online networking destinations like Twitter, Yahoo, Facebook on your site.
Makes alluring, bright, attractive subjects and layout outline.
Topics and layouts can be effortlessly altered and can be utilized on your site.
More than 50 installment passages can be coordinated.
Simple route of items through class.
Multi-store advancement
Bolster and Maintenance
Multilingual and Multi-money include
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SEO agreeable and easy to understand.
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Buy Instagram Likes Through Online At Reputable Website
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Offshore Supply Administrations
At the point when stuck Offshore with no coordinations bolster and no substitution marine extra parts, one can just lament not having profited Offshore Supply Services. In spite of the fact that the mariners as a rule convey the imperative gear with them to confront any circumstance ahead, yet chances are there that specific supplies may abandon. Anyway, when on board, one can't be certain that hardware required at specific minute would be accessible immediately. To get rid of this issue, one must contact organizations that offer Offshore Supply Services. The organizations offering Offshore Supply Services are known as Ship Chandlers. They represent considerable authority in satisfying every one of the necessities of the group individuals and other individuals on the ship.
Why Avail Offshore Supply Services?
Benefiting Offshore Supply Services is of high significance for each one of the individuals who need to cruise through conduits. Here is more on the reasons why Offshore Supply Services ought to be profited.
The organizations giving Offshore Supply Services keep up a colossal load of marine items, transport supplies and extra parts. The general population on load up are furnished with whatever they require, on time.
Ships require support and repairs. On their entry on port, the Ship Chandlers offer all the imperative repair and support administrations. They have an endless system over all the real ports and in this way can be drawn nearer anyplace. In the event of any basic repair employments, specialized help from the designers and different specialists of Ship Chandling Company is made accessible to the group individuals.
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Send Chandlers offering Offshore Supply Services guarantee safe stacking and emptying of the boats Offshore. Accordingly, if the ship proprietors are shy of labor, they can simply rely on Ship Chandling Services giving organizations.
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Aside from giving the previously mentioned backing and administrations, Ship Chandlers additionally care for:
Taking care of Anchor in various water profundities
Introducing substantial mooring and presets
Helping sub sea base
Transporting gear to and from apparatuses and generation units
Supporting Offshore crane vessels
Visit our site to know more about RAK FTZ Offshore company.
Purchase Accessories Through Online For Your Apple Products
People struggle to purchase accessories for their gadgets. For different models they designed different accessories like charger, headset and others. You need to check it and buy it. But people prefer to purchase through online; if accessories won't suit their gadget then they can't return it. But when you purchased from mobile universe then you can gain return back policy. They offer you return back policy, so you can return it in case it won't set your gadget. They offer audio system for your phone as well as for your cars too. You can get accessories for your apple products too, so visit their home page in order to place your order. You can visit www.mobile-universe.ch/apple/ for purchasing accessories for your apple phone. They are selling accessories for different models of apple phone, so filter your search and select the accessories, which suit your phone.
Products At Reasonable Rates
They are selling products at moderate price. Discounts are also there for some products so purchase for the latest Apple creations and products at discounted price and save your money. They will keep updating the products, when new arrivals enter into the market. They will keep on updating the accessories, so you can see new arrivals in their site. Individuals prefer new arrivals in accessories; in order to satisfy them they are selling new arrivals. Especially in case cover, you can able to see different colors, which are attractive. Different models of case cover and which are highly standard. Especially, for your apple phone, you can see wide range of accessories. You can keep on changing the case cover, when new arrival enters the market.
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Try out case covers in different varieties and in designs. You can purchase your favorite colors too, since they are selling covers in various colors. You can purchase it and change the cover frequently. Based on your dress color you can change than cover. Purchase covers in different colors and change it daily based on your dress color. They are selling variety of accessories for your Smartphone and iphones, so visit their site for purchasing it. Purchasing accessories for your smart phones is not a struggle for you.
Benefits and best advantages of Kiosks for the public
Kiosks are the multifunctional device which can be easily seen in various parts of the places. It is a very useful device which helps the public to solve the problem instantly. It is operated by the computer and fully optimized. You can see at the hospital, airport, play station, market, and school. You can see various kind of kiosk device all over the world. it is a hardware and software device both and functioned for multiple purposes. It is a simple device and anyone can easily operate it. It is a simple device and works on a large touch screen or keyboard. As the generation changing, devices are also changing its whole working system. It works on large touch screen instead of keyboard.
You can easily operate the kiosk without any trouble. Public is finding kiosk an excellent operating device and completely depends upon it. Its best services and benefits attract the public towards it. Olea Kiosks are one of them. Kiosk automatically updates the information and helps you to keep your site fresh, up to date and accurate. videos, images or text can be easily updated through content management tools. It helps you to create your own surveys by the customer's opinion.
Advantages of kiosks-
- Anywhere anytime: the best advantage of kiosks is to use it anywhere and anytime. Public are totally depend upon the kiosks machine. If they get any technical problem they find the help through kiosks machine. You can find this device everywhere. You can do lots of things through this device. You can do the work of banking, hospitality, educational, or playing games.
- A simple operating machine: using the kiosks is so simple. Anyone can use it without having any trouble. Any kid, young or old can operate it without the help of any other. Public are now have become expert in using the kiosks device. if any individual is alone he can use it without any problem because its functions are so simple and easily understandable. You can use the touch screen or keyboard according to your preference.
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The upper given advantages affects of kiosks can easily solves the public's problems. Olea Kiosks also provides the best advantages to the public. You can also have the advantages and contact us anytime from anywhere.
Office Phone Systems, A Complete Contrast Of The Various Alternatives Available
In this day and age the phone has really wound up being the most essential medium of correspondence. Phones are regularly utilized everywhere throughout the world mostly for private utilization or might be for working environment use. If there should be an occurrence of Edmonton business phone systems, the framework is designed to the point that it allows its client to share the exceptionally same outside phone lines instead of using particular phones. These phone frameworks are basically created for various telephone clients at a solitary zone. By utilizing these sorts of telephone frameworks incredible arrangements of money can be monitored as it is greatly taken a toll effective in contrast with other telephone frameworks.
The Private Branch Exchange or the PBX telephone frameworks are phone frameworks which are created particularly for business purposes in work environments. By using a PBX telephone framework it is truly easy to contact somebody inside the telephone framework by calling only a three or four digit expansion. The individual branch trades for office telephone frameworks are in like manner equipped for noting the telephones and welcome their customers with auto-created messages and there is likewise a decision for live call moving in this framework.
The Automated Attendant is a telephone framework gadget that can answer the telephone calls by creating robotized messages electronically. An ACD or Automated Call Distributor is a telephone framework gadget which serves to way approaching calls amongst an arrangement of augmentations and oversees them viably. The CTIs are utilized to interface a telephone framework to a PC.
The work environment phone frameworks are incorporated with standard simple phones, copy machine, cell and cordless telephones with the conventional phone system furthermore have the decision for the Internet by Voice over IP. The working environment telephone frameworks have turned out to be unbelievably prevalent as they can convey a straightforward configurability association with unmatched costs.
By using these sorts of telephone frameworks bunches of money can be moderated as it is extremely fetched effective as opposed to whatever other telephone frameworks.
By utilizing a PBX telephone framework it is truly easy to achieve some person inside the telephone framework by dialing just a 3 or four digit expansion. The individual branch trades for working environment telephone frameworks are in like manner fit for tending to the telephones and welcome their clients with auto-created messages and there is likewise a decision for live call moving in this framework.
The Automated Attendant is a telephone framework gadget that can react to the telephone calls by making programmed messages digitally.


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7817 Cedar Lake Road, Edmore 48829
See more homes for sale in Edmore, MI
Brick home on over 18 acres of property! Own you own piece of paradise. Approximately 12 acres of woods with trails rolling land, mature trees plus approximately four acres of pasture land. Home features four bedrooms, den, formal dining, partially finished basement and four season room. Also, two pole buildings, small hip roof barn and sauna building (log). Home warranty included. This property has a lot to offer, a must see!
Property Information
- Status
- Contingency [?]
- Address
- 7817 Cedar Lake Road
- City
- Edmore
- Zip
- 48829
- County
- Montcalm
- Township
- Home
- Possession
- At Closing
- Zoning
- Agricultural, Residential
- Listing Date
- 03/13/2016
- Total Finished SqFt
- 2,600
- Lower Finished SqFt
- 750
- Above Grade SqFt
- 1,850
- Garage
- 1.0
- Garage Desc.
- Attached, Concrete Floors, Door Opener
- Waterfront Desc
- None
- Water
- Private Well
- Sewer
- Private Septic
- Year Built
- 1950
- Home Style
- 1 Story, Raised Ranch
- Taxes
- $3,960
- Summer Taxes
- $838
- Winter Taxes
- $3,121
Rooms and Land
- MasterBedroom
- 12.6X13 1st Floor
- Bedroom2
- 106X11.6 1st Floor
- Bedroom3
- 11X11.8 1st Floor
- Bedroom4
- 11.4X12.4 1st Floor
- Dining
- 12X19 1st Floor
- Family
- 24.1X20.2 Lower Floor
- Kitchen
- 12.6X12 1st Floor
- Laundry
- 12X12 Lower Floor
- Living
- 18.10X15.9 1st Floor
- Other
- 12.3X11 1st Floor
- Basement
- Block, Entrance Outside, Finished Rooms
- Cooling
- Baseboard, Central Air, Natural Gas
- Heating
- Baseboard, Central Air, Natural Gas
- Acreage
- 18.23
- Lot Dimensions
- See Associated Docs.
- Appliances
- Curtain Rods, Dishwasher, Drapes, Dryer, Exhaust Fan, Microwave, Natural Gas Water Heater, Oven/Range, Refrigerator, Smoke Alarms(s), Washer
- Fireplace Desc.
- Fireplace(s), Gas, Insert, Stove, Wood
- Interior Features
- Built-In Bookcase, Den/Study, Formal Dining Room, Foyer Entrance, Mud Room, Sauna, Workshop
- Exterior Materials
- Brick
- Exterior Features
- Countryside View, Sidewalk
Listing Video for 7817 Cedar Lake Road, Edmore MI 48829
Mortgage Calculator
Listing Broker
Listing Courtesy of Century 21 Lee-Mac Realty
Office Address 605 W WARWICK DRIVE
Listings last updated: . Some properties that appear for sale on this web site may subsequently have been sold and may no longer be available.
Our Michigan real estate agents can answer all of your questions about 7817 Cedar Lake Road, Edmore MI 48829. Real Estate One, Max Broock Realtors, and J&J Realtors are part of the Real Estate One Family of Companies and dominate the Edmore, Michigan real estate market. To sell or buy a home in Edmore, Michigan, contact our real estate agents as we know the Edmore, Michigan real estate market better than anyone with over 100 years of experience in Edmore, Michigan real estate for sale.
The data relating to real estate for sale on this web site appears in part from the IDX programs of our Multiple Listing Services. Real Estate listings held by brokerage firms other than Real Estate One includes the name and address of the listing broker where available.
IDX information is provided exclusively for consumers personal, non-commercial use and may not be used for any purpose other than to identify prospective properties consumers may be interested in purchasing.
Nothern Great Lakes REALTORS® MLS. All rights reserved.


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Sieps är en statlig myndighet som tar fram forskningsbaserade analyser i europapolitiska frågor. Målgruppen är i första hand svenska beslutsfattare på olika nivåer. Arbetet sker i samarbete med svenska och internationella forskare.
Avancerad sök
Regional Integration and Regional Trade Agreements
International trade has undergone liberalisation in the post-war period. This liberalisation has been undertaken globally, through the GATT and the WTO, but also at the regional level. In the last ten years the number of regional trade agreements has increased dramatically. It would appear that regional agreements will continue to play an ever more important role. The EU is an important actor in this regard, having a plethora of trade agreements with many different countries.
The report looks at the motives and the consequences of this regional emphasis. Regional trade agreements lead to larger and more integrated markets where economies of scale can be utilised. Furthermore, regional trade agreements might lead to a domino effect, since there are incentives for outsiders to deepen their co-operation with regions to exploit the economies of scale.
The report surveys the empirical literature on regional economic integration and concludes that regional trade agreements benefit the contracting parties, but that the effect on countries that are outsiders are negligible.
The report was presented at the seminar Conflict of Interests in the World Trading System - Global T and is a part of the research project Trade and Welfare Effects of the EU's Bilateral and Regional Trade Agreements.
2004:1 Regional integration och regionala handelsavtal (717.86 kB)


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How Do I Charge For This Tasting?
Business By cakiemommie Updated 5 Apr 2010 , 1:02am by ladyonzlake
Hello! Ok, so I have a bride who wants a football helmet grooms cake. great. but she wants a tasting.. I'm thinking the cake will cost between $150 and $200. How do I charge for the tasting? I told her 3 flavors and fillings.. she doesn't want traditional flavors like chocolate or vanilla, but wants to try my 'gourmet' offerings.. smores, peanut butter n jelly, pink champagne cake, etc...
How do I charge for this? Usually I only do tastings for cakes $300+ and i do them for free. but for a cake that may only be $150 I've got to charge right?
also, keep in mind that she hasn't booked with me yet.. so if she pays for the tasting, likes it and wants to book, should I use the cost of the tasting towards her deposit?
Thanks everyone!!!!
Well you can either charge her for the tasting and then apply it to the balance if she books with you (that's what I do) or tell her you only give free tastings for cakes that are over a certain amount and that her cake will not cost that much.
If she wants to purchase a small cake(s) in the flavor(s) she wants to try she can do that.
I would charge extra for the gourmet flavors, btw
Well, of course she wants a tasting....it's free cake. Everybody wants free cake. But, let me ask you this, have you ever gone into a nice steak house and said, "I am thinking of ordering that nice $38 dollar filet. I like it medium rare. Why don't you go back, have the chef grill it up, I'll take a bite, and if I like it, I'll pay you for it, but if not, I am going to go to Ruth Chris."
Real businesses have policies that keep them profitable or they go out of business. A three flavor tasting a $150 cake is insane. She is taking advantage of you. The next time you have a couple of cakes on the books, take a little extra better, and bake it into a cupcake. That's her sample. Other samples need to be ordered as cakes. I make them order a 6 inch round, but discount it to $28 since there's not much decoration with a sample.
I agree with snarkybaker (great name, BTW!). Don't let them dictate to you what they taste unless you're prepared to get paid well.
My consultations get what's on the tray. If those flavors are moist and delicious, then chances are all my cake flavors are great.
I agree with everyone here. Tell her you do tastings for weddings only but you'd be more than happy to sell her a 6" cake for $xx.xx


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Mom's Best Coleslaw Recipe
My Mom created this recipe when I was a child, and I tweaked it a bit to my liking. It's the only coleslaw I will eat.
- 1-1/2 cups shredded cabbage
- 2 tablespoons shredded carrot
- 1 tablespoon chopped sweet red pepper
- 1 tablespoon chopped green pepper
- 2 tablespoons plus 1 teaspoon reduced-fat sour cream
- 1-1/2 teaspoons reduced-fat mayonnaise
- 1 teaspoon red wine vinegar
- 1/2 teaspoon sugar
- 1/4 teaspoon celery seed
- 1/4 teaspoon salt
- 1. In a large bowl, combine the cabbage, carrots and peppers. In a small bowl, whisk the sour cream, mayonnaise, vinegar, sugar, celery seed and salt. Pour over cabbage mixture; toss to coat. Chill until serving. Yield: 2 servings.
Nutritional Facts
3/4 cup: 65 calories, 3g fat (1g saturated fat), 8mg cholesterol, 357mg sodium, 7g carbohydrate (3g sugars, 2g fiber), 2g protein. Diabetic Exchanges: 1 vegetable, 1 fat.
Reviews for Mom's Best Coleslaw
Average Rating
© 2017 RDA Enthusiast Brands, LLC


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- Location:
- Melbourne, Australia
- Price:
- No price
- Manufacturer:
- Alfa-Laval food processing
- Model:
Top Seller
Interested in this machine?
Verified Seller
- Condition:
- used
- Stock number:
- 08693
- Category:
- Food Processing in Australia
- Subcategory:
- Food equipment
- Subcategory 2:
- Dairy equipment
Alfa Laval ALSHAPE cheese block former, used to form blocks of cheddar cheese, can be used to form blocks of soap, and any semi hard products. Shape size is 100 X 50x25mm. Unit is completely automatic and has an out feed conveyor attached.
Unit is located in Perth and was last used on Soap.
Get email updates for Alfa-Laval ALSHAPE


text:
March 17, 2016
All activities of HANYC are carried out under the leadership and direction of the President and Chief Executive Officer in accordance with the directions of the Board of the Association. Candidates for the position must have excellent leadership skills; experience in dealing with local and state governmental agencies and legislative bodies; experience with crisis management; excellent communication skills; and excellent negotiation skills. It is preferred that candidates also have the specific skill sets described below and reside in the tri-state region of New York, New Jersey and Connecticut.
Please Click Here to access the full Job Description
Submit resume and cover letter on or before April 20, 2016 by mail or e-mail to:
DMS Inc.
322 8th Avenue, Suite 702
New York, NY 10001


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Duration: 4min 53sec Views: 10 391 Submitted: 1 year ago Submitted by:
Description: Sexy thing in her lingerie is on the sofa and she is resting her head on the pillows while her pussy is getting plowed. Shay Sights smiles when she finally reaches her climax from all the ramming today by the big erection.
Channel: Brazzers Network
Pornstars: Shay Sights


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ZbrushWorkshops - Comprehensive Introduction to ZBrush 4 | 2.17 GB
English | MP4 | 800x608 | 15 fps | AVC 250 kbps | AAC 83 kbps
Genre: eLearning
This workshop starts from the ground up and is perfect for beginners looking to get started in ZBrush as well as more advanced users who want to dive into the nuts and bolts of the program.You begin by learning how to navigate around the interface and slowly build up your sculpting skills. Gradually more complicated tools are introduced as you develop your proficiency with ZBrush.
To help you access all the information in this workshop it is divided into 6 sections:
•Getting Started
•Understanding the Sculpting Brushes
•Working with Your Model
•Texture Painting with a focus on Spotlight
•Materials, Lighting and Rendering
•Animation and Timeline
*** Resource files are included
Download from Hotfile
Download from FileServe
Download from FileSonic


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We get tons of weird looks, im 21, im a business owner, a bartender, full time student, take care of my family (no kids, i mean relatives), i pay bills, still act my age when im relaxing but im always told im very mature for my age that its weird. He is 33, isn't immature but never enjoyed his young days due to how tough his life was as a kid so we meet in the middle? but can it actually work?
Most Helpful Guy
Oh sure. Yes, definitely. If you have enough in common and are interested in one another then absolutely! I'm about to be 30 tomorrow (Jesus...) and I know some 20-year-old women I'd gladly give a shot. You just have to work out the different priorities you may have but if you can iron that out you absolutely have a chance. If you like this cat, go for it. There is no reason to say this is impossible. Now if you were 18 then I'd say no go. But you're not. You're 21. Still pretty young but undeniably an adult. You have every reason to give this a shot. I hope it works out.11|00|0
thank you!


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Happy snake yearCategory: Photo frames >Events > New Year
Whole-heartedly wish you good luck and a Happy New Year and hope you enjoy to decorate your photos by "Happy snake year", our joyful photo frame.
From disk
From web
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