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22nd Annual Empowering Women of Color Conference
*on-line registration available*
This Year's theme: "Our Bodies, Our Souls: Sistahood,
Health and Healing" seeks to explore the themes of
health and wellness as they pertain to women of color
and our communities. Healthy minds, bodies and spirits
enable women to come together as change makers to health
and build a world in which their work is sustained.
Conference events will include a health fair scheduled to
take place on UC Berkeley campus, Friday March 2nd.
The main conference event will take place on Saturday
March 3rd and will include a panel of acclaimed Bay Area
activists and leaders in community health and women's issues,
vendors, cultural performances, workshops on a variety of
health and spirituality-related topics. The keynote speaker
will be Mililani Trask, a renowned leader of the Hawaiian
sovereignty movement and an internationally acclaimed
political speaker and attorney.
Conference events will be held in the Martin Luther King,
Jr. (MLK) Student Union Building- March 2 and 3rd, on the
University of California, Berkeley campus. MLK is located
on the northwest corner of the intersection of Telegraph
Ave. and Bancroft Ave. For more information, see
UC Berkeley'scampus map or directions to Campus.
Wheelchair accessible.Registration for the
conference will take place on-site March 3rd.
For additional information,
visit http://ewocc.berkeley.edu
Brought to you by the Graduate Women's Project at the
University of California, Berkeley of the
Graduate Assembly.
This is a blog of resources about the emerging mothers movement as well as my personal thoughts about being a feminist mother. My aim is to help make mothers a more vocal part of the women's movement. Mothers rights are women's rights!
Tuesday, January 09, 2007
Empowering Women of Color Conference at Berkeley: Call for Participants
Posted by Feminist Mom at 4:41 PM 1 comment:
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|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|Cliff Woolley <cliffwool...@yahoo.com>
|Re: cvs commit: httpd-2.0/server/mpm/worker worker.c
|Wed, 19 Sep 2001 15:58:42 GMT
[moving this back on-list] On Wed, 19 Sep 2001, Aaron Bannert wrote: > On Wed, Sep 19, 2001 at 11:45:57AM -0400, Cliff Woolley wrote: > > Anyway, I'm of mixed feelings about the (void) thing, since sometimes we > > do in fact get bugs from having thrown away a return value that we > > shouldn't have thrown away. But then again having the (void) in there > > doesn't make it any more obvious that THAT's where the bug is, I think... > > who knows. > > I could really go either way. I've become more accustomed to keeping > the (void), for readability/maintainability. Just out of curiosity, > how would a deliberate (void) reveal bugs? I'm not seeing it. It wouldn't, that's my point. We often get bugs from discarding an error that got returned by some function. Having the (void) in there makes it more clear that we're discarding a return value to someone who doesn't know the function returns a value at all, but it doesn't really help find the bug. Why? You see some bug, so you go into the debugger. You start stepping through code. You see an error condition happen in some function. You see that error get ignored by the caller. That's where your bug is. The fact that the caller said (void) doesn't help you at all. Oh well, I don't care that much. I don't think the (request_rec *)NULL needs to be in there (and I doubt anyone else in the group does either), but I could be convinced either way on the (void) thing. If someone feels strongly about it, just give me a -1 and I'll put the (void)'s back. --Cliff -------------------------------------------------------------- Cliff Woolley email@example.com Charlottesville, VA
|View raw message

subset: commoncrawl


text:
A Delta Air Lines flight was diverted over the weekend when a Samsung device overheated after being jammed between seats. But the device wasn't the recently recalled Samsung Note 7 smartphone, it was an unspecified tablet from the device maker. [More]
Overheating Samsung Tablet Blamed For Delta Flight Diversion
By September 26, 2016
Google's Echo Competitor Will Be "Google Home"
By May 18, 2016
HP Starts The Tablet Smack Talk, Claiming Theirs Will Beat iPad
By May 25, 2011
We can just hear it now: "Your tablet is so stupid, it thought a quarterback was a refund!" Hewlett-Packard hasn't released their TouchPad tablet computer yet, but already they're smack-talking iPad and Android devices and claiming it'll be the best one ever in the whole entire world. So there!


text:
March 17, 2016
Like this podcast? rabble is reader-supported journalism. Chip in to keep stories like these coming.
Labour updates of workers' struggles from the Asia-Pacific region.
Interview with Lisa Maza, spokesperson of Gabriela, a feminist women's organization in the Philippines, on the struggle for gender equality in the Philippines.
Asia Pacific Currents is a program of Australia Asia Worker Links.
APC is produced in the Melbourne studios of 3CR Radio.
We welcome your comments! rabble.ca embraces a pro-human rights, pro-feminist, anti-racist, queer-positive, anti-imperialist and pro-labour stance, and encourages discussions which develop progressive thought. Our full comment policy can be found here. Learn more about Disqus on rabble.ca and your privacy here. Please keep in mind:
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text:
There are 13 real estate agents servicing Newtown and surrounds. In 2014 they sold 186 properties. We have analysed all these Newtown agents and on request within 24 hours we will send you a free, up-to-date report on their performance, sales track record and what fees you should pay. View report contents
We are the only service in Australia that analyses all local agents and their performance, and provides this to you in a transparent and unbiased manner. View frequently asked questions
We pride ourselves on providing independent, insightful analysis on real estate agents. Read real client case studies to see how we continually exceed expectations. We never disclose your details to any agents unless you specifically instruct us to do so.
We Research All Agents
- Comparable properties sold
- Prices achieved
- Days on market
You Compare, You Select
- Report Emailed in 24 Hours
- You Review Agent Performance
- You Select Any Agent(s)
We Introduce, Property Sold!
- Your Top Agents Call You
- Time, Stress, Risk Reduced
- Property Sold
Which Real Estat Agent is an Australian company (ACN 092 013 931) established in 2011. We provide professional, free services to property sellers Australia wide, with operations in Sydney & Melbourne.
1/246 Oxford Street Paddington NSW 2021 | 1300 66 555 7 | email@example.com
Real Estate Agents Newtown – 2012/13 Performance
Newtown Real Estate Agents sold 186 properties over the last 12 months (145 houses and 41 units). On average these 145 Newtown houses took 72 days to sell and were sold at an average discount of -7% from their initial listing price. Newtown units on average took 63 days to sell and were sold at an average discount of -6% from their initial listing price.
The best Newtown Real Estate Agents sell properties considerably better than these average figures. We detail who these Newtown agents are in our free report
Importantly it is the performance of the individual real estate agent rather than the agency used that matters. With over 13 agents operating in the Newtown council area servicing the Newtown market and 5 agencies, vendors should only use those Newtown agents who routinely deliver superior results for their clients. This is crucial to maximise their chances of securing the best possible price for their Newtown property.
With total house growth of 38% over the last five years Newtown agents have had it reasonably easy selling into an appreciating market. Units have fared better growing at 45%. Growth in Newtown houses over the last year has been below the five year annual growth rate, coming in at -1% for houses (5yr average 8%) and above for units 12% (5yr average 9%).
Request your free report for the individual performance details of real estate agents in Newtown and the properties they have sold over the last couple of years.
With Newtown houses only selling on average every 11 years and units every 9 years, securing the best Newtown real estate agent to manage this infrequent transaction is crucial.
At the end of the day choosing the best Newtown real estate agent to sell your property can make years of difference to your personal financial situation.
Suburb Overview
Newtown is an inner western suburb of Geelong, Victoria, Australia. It is a primarily residential area occupying one of the highest points of urban Geelong, has always been a desirable place of residence and it is the location of many of Geelong's oldest and most valuable properties.
The locality of Chilwell is part of Newtown, and together Newtown and Chilwell were a municipality from 1858 to 1993 after which they were amalgamated with other municipalities to form the City of Greater Geelong in 1993.
Chilwell Post Office opened here on 1 April 1912. Bareena Post Office opened in 1911 in the area and remains open.
The Geelong Highland Gathering is an annual Highland Games held at Queens Park and first began in the 1850s.
Queens Park is also the home ground for the Newtown and Chilwell Cricket Club, the most successful team in the Geelong Cricket Association in terms of senior premierships won. The club uses both Stinton Oval and Shaw Oval. The Queens Park Sporting Complex is currently being refurbished by the City of Greater Geelong and will greatly improve both playing and social facilities for the club. Famous cricketers who played for Newtown include the young Lindsay Hassett, Aaron Finch and Ian Redpath. Bob Merriman, the former Chairman of Cricket Australia, began his administrative career at Newtown and Chilwell Cricket Club.
The suburb is also home to the Geelong Amateur Football Club who also use Stinton Oval during the winter months. They play in the Bellarine Football League.
The suburb in conjunction with neighbouring area Chilwell has an Australian Rules football team competing in the Geelong Football League, as also does Saint Josephs.
Queens Park, located on the Barwon River is home to the picturesque 18 hole Queens Park Golf Course, located in a valley between Fyansford, Highton and Newtown.
Newtown has several significant parks, including Queens Park, Balyang Sanctuary, Chilwell Gardens, Windmill Reserve, Fyans Park, Zillah Crawcour Park and Frier Reserve.
Best real estates agents are often very difficult to find, this in-depth guide explains exactly what to look for and highlights some common traps to avoid.more
Ignoring market conditions for a moment and looking purely at the seasonal nature of property transactions in Australia, you may be wondering when is the best time to buy property. This article explains when and why.more
When is the best time of year to sell your property. Somewhat surprisingly the best time to sell your property is ....more
Australia's two largest cities continue their upward march, with property prices increasing around 1% over the last month and 3% over the last quarter. more
Australia's two largest cities continue their upward march, with property prices increasing around 1% over the last month and 3% over the last quarter. more
Building and pest inspection on a building you're buying at an auction is important. It is vital to have its... more


text: Sorting type: Most visited Most visited Alphabetical | This category is for images, organized by copyright status. Subcategories Fair use screenshots Screenshots of video games Retrieved from "http://indianajones.wikia.com/wiki/Category:Images_by_copyright_status?oldid=9518" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Images Add category Cancel Save


text:
Legacy of Rome: (your choice)
Sept 24/2014
period 4
Paragraph explaining your choice for Rome's most important Legacy.
Your paragraph should explain the legacy you chose, why it was important to Rome, and how we use it today (the impact)
You can use Google to search your topic or you can use your textbook pg. 67-73


text:
©2008 Saland Publishing; (P)2008 Saland Publishing
Full of romance, in all its relativity and variety. His works are just completely, beyond meaning and comprehension. Uplifting and meaningful!
Report Inappropriate Content
If you find this review inappropriate and think it should be removed from our site, let us know. This report will be reviewed by Audible and we will take appropriate action.


text:
Ngozi Achebe was born in London and raised in Nigeria in a middleclass family; the daughter of Augustine, a civil engineer and Matilda, a nurse. Her uncle is Chinua Achebe, author, Professor at Brown University, and critic, best known for his book Things Fall Apart (1958), which is the most widely read book in modern African literature.
Her early unpublished writings were about the darkness of war and survival having been one of the children that lived through the Biafran war - a catastrophic event that engulfed 1960's Nigeria and a potent definer of many childhood memories.
She currently lives in Olympia, Washington and is a practicing physician.
What two or three things would you do differently if you were starting your publishing career today?
I am just starting. I wish I had started earlier though.
What one issue makes you struggle the most as an author? How do you handle it?
Finding time as a mother, physician and teacher to do my writing. But it is a struggle I enjoy. I'd rather have too much to do any day than not have enough.
What is the best writing (or life) advice you have ever heard or wished you had followed? Why?
Don't have ceremonies around your writing. Just do it.
What one issue ignites your passion? Does your passion fuel your writing? What would you do with your life if you didn't write?
Ordinary people doing extraordinary things without knowing it and me writing about it. My characters are inspired by real life people I meet casually or don't even meet at all!
Tell us a bit about your current project.
I'm writing a coming of age novel of a child growing up in a war-torn country. I'm trying to inject some humor into it because there can be humor in adversity.
Do you still experience self-doubts regarding your work, or struggle in a particular area such as writers block or angst driven head-banging against walls? Please share some helpful overcoming hints that you've discovered.
Absolutely. Sometimes I look at my work and think about more effective ways I could have said something. It can drive you mad sometimes!
What is your favorite source for finding story ideas?
People and things around me. I know it sounds corny but there's an old English saying that goes like this There's nowt as strange as folke. We are all a little strange in our own way. That strangeness is my inspiration.
Share a dream or something you'd love to accomplish through your writing career.
I would like this book Onaedo The Blacksmiths Daughter to be read by students of history especially in Nigeria, in Africa.
What gives you the greatest writer buzz, makes the trip worth the hassles (besides coffee or other substances, or course )?
Seeing the finished product- The Book. Its almost like giving birth.
What is one of the more unique or strange life experiences that has really given you an extra oomph in your writing?
Being the survivor in the late sixties and early seventies of a three year civil war in Nigeria as a child. My siblings and I went through things no child should go through, living constantly in the shadow of death. I have always been drawn to make allusions to that period of my life in all my writing.
Describe your special or favorite writing spot or send a picture if you'd like.
My kitchen island. I can raid the fridge when I get writer's block!
What is the first thing you do when you begin a new book?
Write down everything that comes into my head. I'm not as organized in my writing as I am in my practice of medicine
Plot, seat of pants or combination?
Usually seat of pants.
Have you received a particularly memorable reader response or peer honor? Please share.
My uncle Chinua Achebe the world renowned author of many books including his most famous Things Fall Apart, is called the godfather of African literature. A reader once compared me favorably with him. To me it was like being compared to Shakespeare.
Have you discovered any successful marketing/promo ideas that you'd share with us?
Not yet. Ask me again in two years.
Friday, January 07, 2011
Author Ngozi Achebe ~ Interviewed
6 comments:
Thanks you for sharing your journey with us, Ngozi.ReplyDelete
- Anonymous7:07 AM, January 07, 2011
Wonderful interview. Thank you.ReplyDelete
Could not but notice that you stated that "Ngozi comes from a middle class background". Spoke to several Nigerian and West African (Ghanaian) friends here in the States. There is nothing "middle class" about Ngozi's background. Nigeria percapita income is $2300 (2008 est.)compared to the USA's which is ~$48,000. One can not use Western parameters to talk about others outside the West. Most likely her parents and most definitely her uncle who is a national hero are elite members of Nigerian society, therefore, members of the upper class. We often use Western standards to judge others without doing the research. Just FYI
Susan Emevon
Great answers. Impressive life.ReplyDelete
Enjoyed reading about Ngozi and her life in Nigeria and writing journey. Good interview. All the best to you, Ngozi.ReplyDelete
Great interview! I identified with a lot of what Ngozi said about her writing POV... yet her background is completely different from those of us who grew up in the U.S. The book sounds fascinating!ReplyDelete
And anyone else who shares Susan's concern...
all of the Novel Journey author bios are either provided by the author, a publicist/publisher or from the author website/blog information. We don't have time or the resources to do write-ups on our interviewees.
Thanks again for sharing your journey, Ngozi, and for sharing your concerns, Susan.


text: Viewing Super PAC-Men - 4 of 5 The Slogan Guest star Michael Emerson helps Terry and Randy come up with with the perfect slogan for Mitt Romney. Michael Emerson June 12, 2012 17k Views More Info Full Credits Super PAC-Men Uploader Michael Emerson Actor David Marmor Director adamstein Writer/Actor Johnathan McClain Actor ryanhoward Cinematographer Funny Or Die Politics Channel Additional Credits Starring Michael Emerson as The Boss Stats & Data 614Funny178Die 16,754 Views June 12, 2012 Published Category Web Series Sketch Politics Tags politics Mitt Romney president Barack Obama presidential campaign sloganeering advertising Super PAC Republican GOP olives martinis Mad Men Don Draper Michael Emerson Lost Person Of Interest Johnathan McClain Adam Stein David Marmor Ryan Howard Up Next: Rules Of Debate AUTOPLAY Up Next In: 10 Rules Of Debate Santorum Unleashed Santorum Unleashed (AND UNCENSORED!) TERRY AND RANDY AND ROMNEY Follow Browse Related Comments Super PAC-Men 5+ more View All ▶ Politics 760+ more View All ▶ 6+ more View All ▶ Super PAC-Men 6+ more View All ▶ David Marmor 7+ more View All ▶ adamstein 7+ more View All ▶ Johnathan McClain 10+ more View All ▶ ryanhoward 980+ more View All ▶ Funny Or Die Politics Vaguely Related Videos Vaguely Related Pics & Words Advertisement Tags: politics Mitt Romney president Barack Obama presidential campaign Advertisement


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Been here a year. Student Visa maybe? Went to MIT is what local TV is saying (they closed for the day). If so, this may have a huge impact on the immigration bill.
Bingo...the bill is now DOA..
Good thing everyone saved the "che shirt" photo.
It wouldn't surprise me. U.S. "Higher" education highly values foreigners over Americans.
"If so, this may have a huge impact on the immigration bill."
I'm sure it will. Remember, hussein said he would have "more Flexibility" after the election. He'll EO more muzzies into the country. Russia, likechina, would love to see the US go down.
Illegal immigrants; doing the jobs Americans won't do (like blowing up people).
I appreciate your sentiments (or hope which I share) but I don't think it will influence the libs in congress (and some Repubs who wnat to remake the image of the party, however fruitless the effort) from voting for amnesty (by whatever name). And of course, the Demonrats see it, and justly so, as not only a path to citizenship for illegals but as a path to a permanent majority for them. This overrides any concerns they may have for the nation's security.


text: Business & EconomyUS debates financial sector reform Congress considers regulation for consumer protection against financial meltdown. 10 Dec 2009 02:50 GMT | Business & Economy Public anger at big banks hit a high point as the 2008 financial crisis reached its apex, but that anger has ebbed in the months since the cautious recovery of the global economy. At the time, there were widespread calls to regulate financial institutions and protect consumers. The US congress is now considering some of those measures, including a proposed Consumer Financial Protection Agency to rein in banks and protect consumers. Al Jazeera's senior Washington correspondent Rob Reynolds reports.


text:
BELGIUM: EC eyes Portugese grant to VW plant
The European Commission has started a probe into the legality of plans by Portugal to give a EUR3.5m training grant to Volkswagen's Auto Europa car plant.
Get full access to all content, just $1 for 30 days
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Today I can offer you 30 days access for $1 - and that includes just-auto plus.
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text:
With First Tutors: Music you can locate a Thetford euphonium teacher. Euphonium lessons are a great way to begin a stimulating new hobby, pick up a new skill and gain confidence in euphonium. Try our site to search for Thetford euphonium teachers and select which matches your needs so you can begin your euphonium tuition in Thetford straight away!
First Tutors: Music is the fastest way to find Thetford euphonium tutors in the UK, helping you discover Thetford euphonium lessons in your area and Thetford teachers to help with any instrument like piano, violin or guitar. We can help you find Thetford euphonium tuition or a one-to-one Thetford euphonium tutor to prepare for euphonium grades and develop your euphonium skills! We order Thetford euphonium tutors near you according to their tuition prices and distance from you.
If you are a euphonium teacher and would like to offer euphonium tuition services in Thetford or anywhere else across the UK, please register with First Tutors: Music or learn about us here. Becoming a Thetford euphonium tutor is a brilliant way to improve your own knowledge through providing euphonium lessons while assisting someone else, all in Thetford!


text:
Thanks for helping us sort this video!
Thanks for helping us associate the correct Pornstars to this video!
Aidez-nous : ces acteurs/actrices porno apparaissent-ils/elles dans cette vidéo ?
Pornstar Name:
Pick Me, Pick Me........ She is stunning. I think I can help.
Why wouldn't this dude be fucking his wife everyday. She's insanely hot.
She looks like a lotta fun!
Love this sweet looking girl, then she becomes one dirty little slut, having her ass slapped while been fucked. Love this girl. Sweet and Slutty.
v bad video
Some errors occurred, please try again later


text:
Re: Idaho City Yurt Dates
FYIOn Dec 31, 2012 4:28 PM, "Leo Hennessy" <Leo.Hennessy@...> wrote:
Hi Friend,
As most of you know the six Idaho City yurts are back open after being closed 4 months. There are lots of dates available this winter because many people who had initially reserved the yurts this winter made other plans. In January alone, 90 nights are still available. Most of those are weekdays but it would be worth it to take a few days off work to enjoy a world class backcountry adventure. On normal years we have near 100% capacity in January. I am hoping to fill most of the yurts so I can get the yurt program financially stable again after losing 4 months of income. Check out this web link: http://tinyurl.com/a8xa2x5 and it will take you directly to the "dates available" page for all six yurts. If you have any questions please do not hesitate to contact me.
Summer Use: You can reserve 9 months in advance so if you want a specific date this summer now is the time to reserve your yurt. $65 for the weekend and $55 on weekdays.
Have a great winter.
Leo Hennessy
Non-Motorized Trails Program Manager
Idaho Department of Parks and Recreation
PO Box 83720
Boise ID 83720-0065
5657 Warm Springs Avenue
Boise ID 83716-8712
Work: (208) 514-2419
Work Fax: (208) 334-3741
Cell: (208) 866-6471
Web: www.parksandrecreation.idaho.gov


text:
Patrick Lussier and Todd Farmer's next bit of cinematic madness, the Nicolas Cage revenge vehicle Drive Angry in 3D, is officially in the can, and the flick is set to have a big presence at this year's SD Comic-Con so what's left to do? Well, for co-writer Farmer it's time to blog and post images, that's what!
Over on Todd Farmer's official blog the writer has offered a bit of reflection on his experiences.
Dig on a snippet below, and hit up the link for the whole enchilada!
"We couldn't throw big money at the screen to cover up or distract the audience from our challenges," writes Farmer. "We had no choice but to THINK our way out of problems. It's funny but all movies should start with a story. What saved us at every turn was focusing on story. Relying on story telling always bailed us out of any problem where the cats with big money and lots of time rely on big explosions and massive CGI fixes."
Focus on the story? What a novel idea!
Drive Angry, co-written by Lussier and Farmer, centers on a man (Nicolas Cage) driven by rage who is chasing the people who killed his daughter and kidnapped her baby. The vendetta/rescue spins out of control as the chase gets bloodier by the mile, leaving bodies strewn along the highway. Amen.
Look for more on this one as soon as we get it!
Got news? Click here to submit it!
Experience road rage in the Dread Central forums!


text:
Construction progress
Key activities since the last update on 15 March 2017 include:
- Site clearing more than 50% complete
- Construction site facilities installed
- Ergon substation at Kidston civil works completed
- Access road construction underway
- Earthworks underway for the solar farm substation and O&M platform
- Laydown area for materials storage and component assembly 70% complete
- Order placed for the main transformer for the solar farm substation.
The Project remains on budget and on track for First Generation in Q4 2017 and Practical Completion in Q1 2018.
Commenting, Managing Director of Genex Power Michael Addison said, "activity on site continues to ramp up with the construction program well underway. Genex remains very focused on delivering the project on time and on budget. The outlook for wholesale electricity and Large-scale Generation Certificates (LGCs) remains very strong which has the potential to deliver strong early cash flows to Genex".
The Federal Government, through the Australian Renewable Energy Agency has provided $8.9 million funding to support the construction of the $126 million Kidston Solar Farm.
To view figures, please visit:
About Genex Power Ltd
Genex Power Limited (ASX:GNX) is a power generation development company listed on the ASX. The Company is focused on innovative clean energy generation and electricity storage solutions which deliver attractive commercial returns for shareholders. Following Financial Close of Kidston Solar Stage 1 (50MW) Project, the Company's focus will now move onto the development of its 250MW Kidston Pumped Storage Hydro Project and the Kidston Solar Project Phase Two 270MW located at the Kidston Renewable Energy Hub, in Northern Queensland.
Genex Power Ltd


text:
Recipes: mexican chicken & rice dinner, teriyaki shrimp Stir-Fry. Discover more minute® rice meal ideas at www.minuterice.com.
Enriched Precooked Long Grain Rice [Rice, Niacin, Iron (Ferric Orthophosphate), Thiamin (Thiamin Mononitrate), Folic Acid].
Directions. Saucepan: 1. bring water to a boil.* 2. stir in rice; cover. Remove from heat. 3. let stand 5 minutes or until water is absorbed. Fluff with fork. Microwave: 1. place water and rice in microwave-safe bowl.* 2. cover. Microwave on high. Microwave cook times: 2 servings--6 minutes; 4 servings-- 8 minutes; 6 servings--10 minutes. 3. let stand 5 minutes or until water is absorbed. Fluff with fork. Microwave ovens vary. Cooking times are approximate. *If desired, add tub margarine and salt to taste.
Always refer to the actual package for the most accurate information


text:
For all Runners and Endurance Athletes: The scientifically optimized pressure flow provides your muscles with the maximum amount of oxygen and energy.
The times are over where the sole purpose of socks and stockings was to protect feet from blistering. The CEP compression sportsocks are the first functional sport socks with a unique compression profile that provides a scientifically proven increase in blood circulation and a consequent increase in performance.
Achilles Tendon Support: Additional support of the Achilles tendon protects and prevents irritable tendons.
Padded Foot Soles: The special support of the foot sole prevents irritations caused by pressure spots.
Ventilation Channels: For a pleasant foot climate.
3-D Fit: The socks adjust to the anatomy of the leg and foot for a perfect fit thanks to the three-dimensional manufacturing process.
* Shin Splints
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* Blood clots from travel
Color: White, Black, Pink (women's only), NEW Progressive Look in White and Black
NEW Progressive design! Our popular Running Compression sports socks are now available in a new look to match the latest running and triathlon compression collections. - Same compression and features of the Running socks


text:
Dark Blood Vladimir
Dark Blood Vladimir
A pretty cool gothic/noir type of Vladimir skin made by LadyOfBooom, which replaces some of the default abilities and makes them look bit cooler than normally.
In case you have troubles installing the skin or haven't installed Skin Installer Ultimate (SIU) yet, please do check our guide here – http://mobaskins.com/installing-custom-skins/.
Additional Notes
- Custom particles are included
- Created by LadyOfBoom
- Replaces Default Vladimir Skin
The following two tabs change content below.
Admin at MobaSkins
The Chivalrous Admin of MobaSkins, loves long walks on the beach, coach potato and enthusiastic eSports & Gaming fan.
Latest posts by SugiKnight (see all)
- Kirito Yasuo - July 29, 2017
- Reinhardt Sion Skin - May 14, 2017
- Super Saiyan Gohan Ezreal - May 12, 2017
- League Of Legends Auto-Level Script - March 8, 2017
- Free League Of Legends Skins - December 15, 2016


text:
- The Oratorio Society of New Jersey was established in 1952.
- Community-based choral group specializing in the performance of choral masterpieces.
- Choral membership has been as high as 100+ per season.
- OSNJ demographics include adults of all ages residing in Northern New Jersey.
- Volunteer-based organization members volunteer time and effort in supportive activities beyond rehearsals.
- Long history of support from the Essex County Division of Cultural Affairs.
- ALL SINGERS WELCOME! New singers — no auditions!
- REHEARSALS for the Fall 2016 concert will be held at First Lutheran Church, 153 Park Street, Montclair, from 8:00 until 10:00 PM every Tuesday evening starting in early September, 2016. Rehearsals during concert week will be held at the Church of the Immaculate Conception, 30 North Fullerton Avenue, Montclair on Tuesday and Friday from 7:00 until 10:00 PM.
- For additional details, email Susan Ladov.
Our Annual Events are performed at venues in western Essex County.
- Fall Concert typically in mid November.
- Spring Concert typically in March/April.
- Holiday Concert Messiah Sing in December.
In its welcoming spirit, OSNJ does not require auditions. However, it is expected that members have some prior singing experience and make a commitment to master the selections. This involves faithful attendance, and rigorous practice during rehearsal time and on their own. Certain rehearsals also offer the opportunity to learn about historical style, voice production, the vocabulary of music, principles of interval and metric organization, and more.
At the same time, OSNJ's musical activity is meant to be joyous, as well as educational. We consider it part of our charge to create a singing experience that is uplifting and inspiring. Whether working or retired, members can look forward to an environment that momentarily sets the tensions of the day aside.
For information about joining, please email Susan Ladov, President.


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This year's edition of Advanced Night Repair, a powerful anti-aging serum, comes with an exclusive Pink Ribbon Bracelet. With each purchase, 20 percent of the proceeds will be donated to The Breast Cancer Research Foundation, an independent, not-for-profit organization that was founded in 1993 by Evelyn H. Lauder to fund breast cancer research worldwide.
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Dark Stars Photography Photographer
Alicia M WrightModel Female Bellingham, Washington, US
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German luxury car manufacturer has recently launched its 7-Series ActiveHybrid in India. BMW India has now decided to launch a Signature Edition of its 7-Series. The new version by BMW was revealed at the 2014 BMW India Bridal Fashion Week.
BMW India has launched its 7-Series Signature Edition at INR 1,22,00,000 ex-showroom. The vehicle will sport special features and upgrades compared to the regular version. BMW India launched its vehicle at a fashion show lead by Suneet Verma and his bridal wear.
The German-made luxury car will be available only with the 730Ld model of 7-Series. The Signature Edition will sport more creature comfort, along with exclusivity. It will be available in limited numbers and is a great addition into the 7-Series range.
Also Read: Petrol Dips & Diesel Gets Dearer!
The Signature Edition will be powered by a 3.0-litre diesel, twin-turbo V6 engine. It will be capable of churning out 560 Nm of peak torque, along with 258 horsepower. The engine will be mated to an 8-speed automatic gearbox. The 730Ld Signature Edition will sprint from 0 to 100 km/h in 6.2 seconds and achieve a top speed of 250 km/h.
The German luxury vehicle manufacturer will provide its Signature Edition with Active Steering. It also gets rear reading lights and a chiller for drinks. The interior gets Nappa leather and wood accents to give it a premium and exclusive look.
Also Read: Fiat's 2014 Evolutionary Punto Tested!
BMW India will also provide the 7-Series Signature Edition with a Bang & Olufsen surround music system capable of 1200 watts. The vehicle will have 16 speakers within its cabin. It also gets Dirac Dimensions to improve acoustics.
The 7-Series Signature Edition is on sale and is available in all BMW showrooms pan-India. The German luxury automobile manufacturer has priced its Signature Edition at INR 1,22,00,000 ex-showroom. It will be locally assembled and is a marginally more expensive compared to regular 7-Series models.
Don't Miss The Video Of The Day!


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Joe Michell School is an IB Middle Years Programme School
As an IB World School, Joe Michell middle school students study a variety of courses alongside the traditional core curriculum. Students at Michell:
Explore STEM through Project Lead the Way modules in their Design class
Develop a second language and explore cultures from around the globe in year-long Spanish class
Develop new forms of expression in their Visual Arts and Performing Arts classes
Read more to learn about what Joe Michell School has to offer in the 6th, 7th and 8th grade years
Language Acquisition (Spanish)
Joe Michell Middle School grows!
We have added seven new buildings to our middle school in the last two years, including a science laboratory, a design workshop, and an art studio! Thanks to everyone who has supported this growth, and our very own students for helping to make each of our new spaces beautiful!


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First Time Series
First Time Series
Ethan and CarrieEthan hooks up with virgin Carrie.
Exercising with BenefitsHis birthday present is late but he isn't complaining
Expanding HorizonsKeira remembers her first time, en route to reunite with him.
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Exploring SexBookish and busy, Jaime gets an erotic lesson from a friend.
Extra CreditShe delivers his student of choice.
FairgameWhich brother will Emma choose?
FallIt was well worth waiting for.
Fall From GraceThe Corruption Of Catherine.


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Trinamic products offer a variety of unique features to improve accuracy and smoothness of motor operation. Our approach "the motor is the sensor" describes how we utilize electric feedback from the actuator.
The Internet of Things equips us with smart devices. But what makes them smart? Could the Trojan Room Coffee Pot from 1991 already be considered a smart device?
An internet connection isn't an indicator of smartness. Smart people are able to perceive and interact with their environment. The same applies to smart devices. They are equipped with sensors, actuators, and smart algorithms to interact and communicate properly.
As motor control is mainly about actuators, Trinamic products take advantage of the dramatic improvements in sensor technologies. Our motor control devices embed sensors to measure the current load and other key actuator parameters.


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6509 error messeage
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Patching is not available since the system is not running from an installed image. To install please use the "install file" command
We have upgraded the IOS (s72033-ipservices_wan-vz.122-18.SXF9.bin) as per Netpro Community reply.
After IOS upgrade, we have booted the switch with s72033-ipservices_wan-vz.122-18.SXF9.bin.
Still we are getting the above errors.
I have this problem too.
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The Hotel Esmeralda is located at the entrance of the beautiful port of Ciutadella, the former Capital of Menorca, 500 m. from the town, beside the seafront promenade. The rooms are all air conditioned and well decorated. The board basis is all inclusive which means that you have all meals, snacks and local drinks included in the price. The hotel has varied entertainment programme during the day and night to ensure you and your family enjoy an unforgettable holiday. There is a lovely pool with childrens section and sunterrace served by the pool bar or you may choose to take the free bus service to the beach. The town of Ciutadella is really lovely, the old quarter is packed full of charm and history and a great place to have a wander along the winding cobbled streets, visiting some fascinating and original shops, bars and restaurants. You must also wander 450m to the Port of Ciutadella which is bordered by the old town walls and is lined with restaurants. In the evening there are plenty of stalls to take a look at. The Esmeralda is a good hotel, well situated on the edge of a facinating and beautiful town.
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First published September 5, 2001; 10.1152/ ajpcell.00256.2001.—The expression and function of the endogenous inhibitor of cAMP-dependent protein kinase (PKI) in endothelial cells are unknown. In this study, overexpression of rabbit muscle PKI gene into endothelial cells inhibited the cAMP-mediated increase and exacerbated thrombin-induced decrease in endothelial barrier function. We investigated PKI expression in human pulmonary artery (HPAECs), foreskin microvessel (HMECs), and brain microvessel endothelial cells (HBMECs). RT-PCR using specific primers for human PKIα, human PKIγ, and mouse PKIβ sequences detected PKIα and PKIγ mRNA in all three cell types. Sequencing and BLAST analysis indicated that forward and reverse DNA strands for PKIα and PKIγ were of >96% identity with database sequences. RNase protection assays showed protection of the 542 nucleotides in HBMEC and HPAEC PKIα mRNA and 240 nucleotides in HBMEC, HPAEC, and HMEC PKIγ mRNA. Western blot analysis indicated that PKIγ protein was detected in all three cell types, whereas PKIα was found in HBMECs. In summary, endothelial cells from three different vascular beds express PKIα and PKIγ, which may be physiologically important in endothelial barrier function.
- cAMP
- protein kinase inhibitor
- endothelial resistance
- barrier function
vascular-based disorders such as ischemia-reperfusion injury, atherosclerosis, diabetes, and stroke are associated with significant endothelial cell barrier dysfunction. One signaling system, the cAMP-dependent serine/threonine protein kinase (PKA), has a significant and profound effect in the protection against endothelial barrier dysfunction. There is substantial evidence documenting the protective effects of PKA against endothelial barrier dysfunction in a variety of in vitro and in vivo experimental systems in which intracellular cAMP levels were elevated with either cAMP analogs, reagents that stimulate cAMP production (i.e., forskolin), or agents that inhibit its metabolism (i.e., phosphodiesterase inhibitors) (2-4, 14,16, 18, 20, 21, 24). We showed that overexpression of the highly specific PKA inhibitor (PKI) gene in endothelial cells was effective in fully reversing the barrier-enhancing effects of increased cAMP (16), providing direct evidence for the protective role of PKA. However, the cellular and molecular mechanisms by which PKA inhibits endothelial barrier dysfunction remain undefined. Further, the upstream regulation of endothelial PKA in this function is entirely not known.
Several observations indicate that full activation of PKA is under multiple modulatory mechanisms. In the absence of cAMP, PKA exists as an inactive tetramer consisting of two regulatory and two catalytic subunits. Binding of cAMP to the regulatory subunit lowers the affinity by four orders of magnitude, which in turn causes dissociation of the tetramer into a dimer of regulatory subunit and two active monomers of the catalytic subunit (27). Further, full activation of PKA appears to require phosphorylation of its catalytic subunit by a phosphoinositide-dependent protein kinase (5, 6). This latter observation raises the interesting possibility that PKA is regulated by its phosphorylation state, which in turn determines the cell's responsiveness to cAMP.
Another level of modulation of PKA is by endogenous PKI. PKI is a family of isoforms of distinct genes and has widespread tissue distribution (7, 15). This heat-stable protein has high affinity (0.2 nM) and specific binding for the substrate binding site (as pseudosubstrate inhibitor) on the catalytic subunit of PKA (23, 27, 30). The physiological functions of PKI have not been fully established, but PKI has been shown to contain a nuclear export signal for the PKA catalytic subunit (29). Because of its differential isoform distribution in adult tissues and its distinct interactions with the PKA catalytic subunit, PKI is hypothesized to be critical in the modulation of basal PKA activity in general cell activities (7, 9). Although endogenous PKI has not been studied in endothelial cells, it is possible that PKI may be a key modulator of PKA in endothelial cell activities, such as the permeability response. In support of this thesis, we observed that inhibition of PKA resulted in alterations in endothelial cell-cell junctions and actin filament organization, which were accompanied by impairment of the barrier (22).
The goal of this study was to investigate the involvement of PKI in regulation of endothelial permeability and PKI isoform expression in endothelial cells. Results indicated that 1) overexpression of rabbit muscle PKI decreased endothelial barrier restrictiveness, and2) endothelial cells derived from the human brain microvessel, pulmonary artery, and foreskin microvessel expressed predominantly PKIα and PKIγ isoforms. We conclude that PKIα and PKIγ may be important in modulating PKA in the regulation of vascular endothelial barrier function.
Cell Culture
Human dermal (foreskin) microvascular endothelial cells (HMECs) (1) were maintained in culture in MCDB 131 medium, supplemented with 5% fetal bovine serum (FBS; HyClone, Logan, UT), 10 ng/ml human epidermal growth factor (EGF), 1 μg/ml hydrocortisone, 5% penicillin-streptomycin, and 5% l-glutamine. HMECs are an immortalized cell line transformed by simian virus 40 (SV40) large T antigen and have been shown to retain endothelial cell phenotypic and functional characteristics. They exhibit the expected morphological and functional endothelial phenotypes: express and secrete von Willebrand factor, take up acetylated low density lipoprotein (LDL), form tubes when grown in Matrigel, and express CD31 (platelet endothelial cell adhesion molecule-1), CD36, intercellular adhesion molecule-1 (ICAM-1), and CD44 (1). They also bind purified T cells in a regulatable manner and respond to cytokines in a similar way to nontransformed endothelial cells. HMECs were passaged 5–7 days when confluent and were used for studies at population doublings between 25 and 40.
Human brain microvascular endothelial cells (HBMECs) were provided by Dr. K. S. Kim (Children's Hospital, Los Angeles, CA). HBMECs were cultured in RPMI 1640 supplemented with 10% FBS, 10% NuSerum (Becton Dickinson, Bedford, MA), endothelial cell growth supplement (30 μg/ml), heparin (5 U/ml), 1 mM sodium pyruvate, 1 mM MEM nonessential amino acids, 1 mM MEM vitamins, 5% l-glutamine, and 5% penicillin-streptomycin. HBMECs were grown and used at population doublings between 30 and 40. These cells were immortalized by SV40 large T antigen and have been shown to retain endothelial cell phenotypic and functional characteristics (25, 26). The cells were positive for von Willebrand factor, carbonic anhydrase IV, and Ulex Europeus agglutinin I, took up fluorescently labeled acetylated LDL, and expressed γ-glutamyl transpeptidase, demonstrating their brain endothelial cell characteristics. When treated with tumor necrosis factor-α, vascular cell adhesion molecule and ICAM-1 were expressed.
Human pulmonary artery endothelial cells (HPAECs) were purchased from Clonetics (San Diego, CA). These cells have been characterized to be endothelial in origin by the uptake of acetylated LDL and by positive staining for von Willebrand factor. The HPAECs were grown in basal medium containing EGM-2 Bulletkit growth supplement (Clonetics), 10% FBS, and 5% penicillin-streptomycin. HPAECs were cultured to 10–15 population doubling for use in studies.
The transformed renal embryonic kidney cell line, 293 cells (CRL 1573; American Type Culture Collection, Manassas, VA) (13), were maintained in DMEM supplemented with 10% FBS, 2 mMl-glutamine, 100 μg/ml penicillin, 100 U/ml streptomycin, and 10 mg/ml amphotericin B.
The endothelial expression of PKI mRNA was determined from HMECs, HBMECs, and HPAECs by RT-PCR. Total RNA was extracted using an RNA STAT-60 isolation kit (Tel-Test, Friendswood, TX) according to the manufacturer's protocol. RNA concentration was determined by spectrophotometry at an absorbance of 260 nm. RNA integrity was assessed by agarose gel electrophoresis and ethidium bromide staining. The RT-PCR was made using the GeneAmp RNA PCR kit (Perkin Elmer, Branchburg, NJ), and thermal cycling reactions were run in the GeneAmp 2400 PCR System (PE Biosystems, Foster City, CA). Isolated total RNA was subjected to reverse transcription with oligo(dT) primers, generating cDNA copies of the RNA sequence. The subsequent PCR was performed using specific primer sets based on sequences for PKI isoforms, human PKIα, human PKIγ, and mouse PKIβ (Table1). Reaction included a positive control, pAW109 RNA (provided by the kit) and negative control, which was in the absence of murine leukemia virus RT. RT-PCR products were analyzed by agarose gel electrophoresis. The products were subsequently purified, and both forward and reverse DNA strands were sequenced (Research Resources Center, DNA Core Facility, University of Illinois, Chicago, IL) and analyzed using Basic Local Alignment Search Tool (BLAST 2.0, National Center for Biotechnology Information).
RNase Protection Assay
RNase protection assay (RPA) was performed as previously described (10). Probes were allowed to hybridize to target RNA in reactions containing 10.0 μg of total cell RNA and 2.5 × 104 cpm of PKIα or PKIγ antisense probes (seeRiboprobe templates) in 30 μl of hybridization buffer [80% formamide, 0.4 M NaCl, 1 mM EDTA, and 40 mM PIPES (pH 6.4)]. Reactions were heated to 85°C for 5 min and then incubated at 48°C for 12–18 h. After hybridization, 280 μl of RNase digestion buffer [50 mM sodium acetate (pH 4.5) and 2 mM EDTA] were added with 30 units of T1 RNase for all assays, followed by incubation at 30°C for 60 min. Reaction was terminated and RNA precipitated by the addition of 70 μg of yeast transfer RNA and 700 μl of 7% Tri-Reagent (diluted in 100% ethanol). RNA was dissolved in loading buffer [80% formamide, 2 mM EDTA (pH 7.4) containing 0.05% bromophenol blue and 0.05% xylene cyanol], denatured at 85°C for 5 min, and resolved on a 5% acrylamide/8 M urea gel using 89 mM Tris (pH 8.0), 89 mM boric acid, and 2.7 mM EDTA (TBE buffer). Gels were used to prepare autoradiograms for analysis.
Riboprobe templates.
Purified and sequenced RT-PCR products for human PKIα and human PKIγ (see RT-PCR) were ligated into pGEM-T-Easy vector, chemically transformed into DH5α competent cells, and plated onto Luria-Bertani (LB)/ampicillin agar plates at 37°C. A successfully transformed colony (screen by restriction digests) was grown in LB overnight at 37°C, and the plasmid-DNA insert was isolated using the Wizard Plus Minipreps DNA purification system. The plasmid-DNA insert was linearized with PstI (PKIα) orBamHI (PKIγ) and transcribed with T7 RNA polymerase to produce radiolabeled antisense probes.
In vitro transcription of radiolabeled probes.
Probe synthesis was conducted with 1 mM each of CTP, ATP, and UTP, respectively, 9.38 μM of [32P]GTP (800 Ci/mmol), and 25 μM of unlabeled GTP. One microgram of template DNA with appropriate RNA polymerase (T7 RNA polymerase) was added to each reaction and was allowed to transcribe at 37°C for 40 min, and template was digested with DNase (Ambion) at 37°C for 15 min. The [32P]GTP not incorporated into the probe was removed using Centri-Spin 40 purification columns (Princeton Separation, Adelphia, NJ). Radiolabeled probes were used in RNase protection assays to detect mRNA levels of PKIα and PKIγ from total cell RNA isolated from HMECs, HBMECs, and HPAECs.
Protein Expression of PKI Isoforms
Cell collection and preparation.
HMECs, HBMECs, and HPAECs were grown to confluency in 60-mm culture dishes. The cells were placed on ice, washed two times with ice-cold Ca2+- and Mg2+-free PBS, and collected by scraping in RIPA buffer [in mM: 150 NaCl, 1 EDTA, 1 EGTA, 50 Tris · HCl (pH 7.4), 1% Nonidet P-40, 1 NaF, and 1 sodium vanadate, as well as 0.25% sodium deoxycholate, 1 pepstatin A, 1 phenylmethylsulfonyl fluoride, 25 μg/ml leupeptin, and 25 μg/ml aprotinin]. The cell extract was sonicated with 10 pulses using the sonifier (Branson Ultrasonics, Danbury, CT) and was heated to 92°C for 10 min. After being cooled to 4°C, the endothelial cell lysate was centrifuged at 14,000 rpm for 15 min, and the supernatant was collected for protein concentration, which was determined using the bichinchoninic acid protein assay kit with BSA as standard (Pierce, Rockford, IL).
Antibody preparation.
Affinity-purified polyclonal antibody to full-length murine PKIγ was prepared as described previously (7). In brief, purified histidine-tagged PKIγ protein was conjugated to keyhole limpet hemocyanin and used for immunization of rabbits for antibody production. The antiserum raised against PKIγ was affinity purified on nitrocellulose blots bound with the purified fusion protein. In addition, polyclonal anti-peptide antibodies were prepared against peptides for rat PKIα-(5–22) (TTYADFIASGRTGRRNAI) and rat PKIβ-(5–22) (SVISSFASSARAGRRNAL) as previously described (12, 15).
Western blot analysis.
The heat-treated cell lysates were loaded at constant protein concentrations, and the proteins were separated by SDS-polyacrylamide gradient gel electrophoresis. The separated proteins were electrotransferred to nitrocellulose or polyvinylidene difluoride membranes. Nonspecific binding of antibody to membrane was blocked with 5% nonfat dry milk in Tris-buffered saline with 0.05% Tween 20 (TBST). The blocked membrane was then incubated with primary antibodies, diluted in TBST with 1% nonfat dry milk, overnight at 4°C in a rocker. The blot was washed five times with TBST and incubated with anti-rabbit IgG secondary antibody conjugated with horseradish peroxidase, and the bands were detected using the enhanced chemiluminescence kit.
Transendothelial Electrical Resistance
The transendothelial electrical resistance, an index of endothelial barrier function, was determined in real time using the electric cell-substrate impedance sensor (ECIS) system (Applied BioPhysics, Troy, NY) (11, 19). The system consists of one large gold-plated electrode (10−1cm2), eight smaller gold-plated electrodes (10−4 cm2), and a 500-μl well fitted above each small electrode. The small and large electrodes were connected to a phase-sensitive lock-in amplifier, and an alternating current was supplied through the 1 MΩ resistor. The measured electrical impedance or calculated resistance indicates the restriction of current flow through the cell monolayer and thus provides an index of the endothelial barrier function.
For resistance measurement, endothelial cells (105 cells) were plated onto a sterile, fibronectin-coated gold-plated electrode and grown to confluency. The electrode was mounted onto the ECIS system housed within an incubator (maintained at 37°C, 5% CO2, and 100% humidity) and connected to the lock-in amplifier. After a period of 15 min of equilibration, the cells were challenged with reagents according to experimental protocol and resistances were recorded continuously in real time.
Construction of Rabbit Muscle PKI in Endothelial Cells
An E1−, E3− replication-deficient adenovirus containing PKI (AdPKI) was prepared as described previously (16). The 251-bp DNA fragment encoding the complete amino acid sequence of rabbit muscle PKI (8) was subcloned into the shuttle vector pACCMV.pLpA, creating pACCMV-PKI. Equimolar amounts of pACCMV-PKI (0.2 μg) were cotransfected with the plasmid pJM17 (0.8 μg), which contains the full-length adenovirus (Ad 5) genome sequences (with a mutant E3 region) as well as ampicillin and tetracycline resistance sequences and a bacterial origin of replication (17), into 293 cells, a transformed renal embryonic kidney cell line (13). Homologous recombination between the two plasmids resulted in an E1−, E3− adenovirus genome that can replicate and packaged into virions only in 293 cells in which E1 function is supplied in trans by integrated, constitutively expressed adenovirus E1 sequences. The vector AdPKI was amplified in 293 cells and its genome confirmed by PCR amplication of contiguous adenovirus/expression cassette sequences. The vector was purified by double cesium chloride ultracentrifugation and exhaustive dialysis against virus suspension buffer (10 mM Tris, 10 mM MgCl2, and 10% glycerol) and was titered and stored at −80°C. For control, the vector AdNull, expressing no transgene, was constructed in a similar manner but without subcloned gene sequences between the cytomegalovirus promoter and the polyadenylation signal.
The following were purchased from GIBCO BRL (Gaithersburg, MD): DH5α competent cells, MCDB 131 medium, DMEM, penicillin-streptomycin,l-glutamine, sodium pyruvate, MEM nonessential amino acids, MEM vitamins; from Sigma Chemical (St. Louis, MO): EGF, hydrocortisone, endothelial cell growth supplement, heparin, T1 RNase, ΦX174 RF DNA/HaeIII fragments; from Promega (Madison, WI): pGEM-T-Easy vector, Wizard Plus Minipreps DNA Purification System, T7 RNA polymerase. All other reagents were obtained as indicated in the text.
Overexpression of PKI Decreases Endothelial Barrier Restrictiveness
The role of PKI in the regulation of endogenous PKA in endothelial barrier function was investigated by overexpression of endothelial cells with AdPKI. HMECs were infected at 100 multiplicity of infection (MOI) of AdPKI for 2 days for the study. We have demonstrated that the use of this protocol for adenovirus infection of HMECs was highly efficient (>95%) for gene transfer (16). The cells were treated with 10 μM forskolin (direct activator of adenylyl cyclase) plus 1 μM IBMX (phosphodiesterase inhibitor) to increase intracellular cAMP levels, and the transendothelial electrical resistance was measured in real time. In control noninfected HMECs, the combination of forskolin plus IBMX significantly increased resistance above baseline (Fig. 1), indicating increased restrictiveness of the endothelial monolayer. However, this increased resistance was abolished in the PKI-overexpressing HMECs (Fig. 1).
We also determined the effects of PKI on mediator-induced changes in endothelial barrier function. HMEC were infected at 100 MOI with AdPKI or control AdNull for 2 days, and the transendothelial electrical resistance, an index of barrier function, was determined in response to 1.0 nM thrombin. Stimulation of HMECs with thrombin caused transient decreases in resistance in which the AdPKI-infected HMECs showed greater maximal decreases in resistance relative to the control AdNull-infected HMECs (Fig.2 A). Figure 2 Bsummarizes the maximal thrombin-mediated resistance decrease from baseline in the controls (noninfected wild-type HMECs and HMECs infected with AdNull) and HMECs infected with AdPKI. The results indicated that, in HMECs infected with AdPKI, the thrombin-mediated resistance decrease was significantly greater (−6,526 ± 494 Ω) than in either control wild-type HMECs or AdNull groups (−4,137 ± 123 and −3,837 ± 163 Ω, respectively).
PKIα, PKIβ, and PKIγ mRNA Expression in Endothelial Cells
For RT-PCR analysis, in HBMECs (Fig.3 A), HPAECs (Fig.3 B), and HMECs (Fig. 3 C), specific primers for PKIα and PKIγ yielded PCR products comparable to the predicted size (542 and 240 bp, respectively; Table 1). In HMECs, primers for PKIβ produced a product greater than the predicted 569 bp (Fig.3 C). The positive control (pAW109 RNA) generated the predicted 302-bp band. All negative controls (absence of RT) showed lack of RT-PCR products, indicating lack of contaminating genomic DNA. The RT-PCR products were purified, and the forward and reverse DNA strands were sequenced. The sequences were subjected to BLAST 2.0 analysis and results are summarized in Table2. The DNA sequences for PKIγ from HBMECs, HPAECs, and HMECs were of >96% identity with the human PKIγ gene database sequences, and showed an expectation value (E) of < 10−101, confirming the expression of PKIγ in these three endothelial cell types. Similarly, the DNA sequences for PKIα from HBMECs were of 100% identity with human PKIα gene database sequences, and the E value was 0.0. However, insufficient purified RT-PCR product was obtained from HPAECs and HMECs for sequencing analysis of PKIα. The sequencing results obtained from PCR products using primers for PKIβ indicated that the DNA sequences corresponded to three genes (human prolyl 4-hydroxylase β-subunit, human thyroid hormone binding protein, and human glutathione transhydrogenase).
We next used RNase protection assay to confirm the expression of PKIα and PKIγ in endothelial cells. Riboprobe templates were generated from purified RT-PCR products for PKIα and PKIγ (seematerials and methods). Total RNA isolated from HBMECs, HPAECs, and HMECs was reacted with the antisense probes. Results indicated that the PKIα antisense probe protected the 542 nucleotides of PKIα mRNA from HPAECs and HBMECs (Fig.4, top). The PKIγ antisense probe protected the 240 nt of PKIγ mRNA from HBMECs, HPAECs, and HMECs (Fig. 4, bottom).
PKI protein expression was investigated using Western blot analyses. Anti-peptide polyclonal antibodies directed against PKIα and PKIβ (15) and affinity-purified polyclonal anti-PKIγ antibody (7) were used for Western blot analysis of HBMEC, HPAEC, and HMEC cell lysates. A strong band at ∼20 kDa was observed for PKIγ from the three endothelial cell types (Fig.5 A). PKIα was detected as a band of ∼12 kDa in HBMECs only; PKIβ was not detected in any of the three cell types (Fig. 5 B). Positive control using testis tissue lysate (15) is shown for PKIα and PKIβ (Fig.5 B).
Expression of the exogenous rabbit muscle PKI gene into endothelial cells inhibited the increase in basal endothelial resistance induced by the cAMP-elevating agents, forskolin and IBMX, providing strong evidence that PKA was responsible for mediating the increase in barrier function. This observation was consistent with our earlier finding that PKI overexpression abolished the protective effects of cAMP-elevating agents (i.e., forskolin, IBMX, and cholera toxin) against a thrombin-induced increase in endothelial permeability (16, 22). Interestingly, PKI overexpression also exacerbated the decrease in thrombin-induced transendothelial resistance. This result suggests that PKA normally functioned to maintain the extent of basal endothelial barrier restrictiveness; thus PKI may be a physiological modulator of PKA activity in the endothelial barrier response. Inhibition of PKA also promotes actin stress fiber formation and loss of catenin (22), supporting the notion that endogenous PKA may be important in regulation of basal cell barrier restrictiveness.
The results from the current study indicate that endothelial cells derived from human brain, pulmonary artery, and foreskin expressed predominantly PKIα and PKIγ. PKIβ mRNA and protein were not detected in any of the endothelial cells. PKIγ mRNA was expressed by all three endothelial cell types as determined by both RT-PCR and RNase protection assays. This message expression corresponded with PKIγ protein expression from the three endothelial cell types. Further, PKIα mRNA expression was observed in the three endothelial cell types when determined by RT-PCR; however, RNase protection assay detected PKIα mRNA for only HPAECs and HBMECs, but not HMECs. This finding may possibly be attributed to relatively low levels of the PKIα message expressed by HMECs, which was detectable with amplification by RT-PCR. PKIα protein expression was detected in HBMECs, but not from HMECs or HPAECs, suggesting low levels or lack of the protein expression by these cells. PKIα has been reported to be predominantly expressed in the cerebral cortex and muscle, whereas PKIβ is more limited to the testis (28). Overall, the results are consistent with observations made by Collins and Uhler (7) who reported that PKIγ was abundant and more widely expressed than either PKIα and PKIβ in tissues, such as heart, skeletal muscle, testis, spleen, lung, liver, and kidney. The more prevalent expression of PKIγ in endothelial cells suggests that this isoform may be important in modulating a broad range of basic cellular activities, including the regulation of basal and mediator-induced changes in endothelial barrier function.
A comparison of the sequences between human PKIα and PKIγ coding regions indicated ∼53% homology, whereas the rabbit muscle PKI used for the functional studies showed 74% and 53% homology with human PKIα and PKIγ, respectively. The amino acid sequences in PKI important for binding to and inhibition of the PKA catalytic subunit have been shown to be relatively conserved among PKI isoforms (7). PKI inhibits PKA through interactions within the substrate binding site of the PKA catalytic subunit, functioning as a competitive inhibitor. Although the overexpression of rabbit muscle PKI into endothelial cells likely inhibited PKA in a similar way to PKIα and PKIγ, the relative potency and efficacy may be different. PKIα is known to have greater binding affinity to the PKA catalytic subunit than PKIγ (inhibition constant K i = 0.073 and 0.44 nm, respectively) (7, 9). The sixfold difference in affinity may be physiologically significant, since the basal level of the catalytic subunit is normally maintained quite low in cells.
It is now known that, in addition to its inhibition potential of PKA, PKI functions to shuttle the PKA catalytic subunit out of the nucleus via a nuclear export signal (NES) (29, 31). All members of the PKI family possess an amino-terminal inhibitory region and a central region containing the NES (7). In general, the least conserved region occurs in the carboxy terminus of the molecule (7). These differences among the isoforms and their differential expressions suggest possible isoform-specific physiological function(s). It remains to be determined whether PKIα and PKIγ have differential functions in endothelial cells.
PKIβ was not detected in the endothelial cells by RT-PCR. One reason may be the use of primer-based mouse DNA sequences, which may not be highly conserved with human PKIβ. However, the human PKIβ gene has not been cloned. Nonetheless, the result was not surprising, since PKIβ expression is mostly limited to testis (28).
In summary, the main findings are that 1) overexpression of rabbit muscle PKI decreased endothelial barrier restrictiveness, and2) endothelial cells derived from human brain microvessel, pulmonary artery, and foreskin microvessel expressed predominantly PKIα and PKIγ isoforms. We conclude that PKIα and PKIγ may be important in modulating PKA in the regulation of vascular endothelial barrier function.
This work was supported by National Heart, Lung, and Blood Institute Grant HL-62649 (H. Lum), the American Heart Association, National (H. Lum), and a Veterans Affairs Grant (C. E. Patterson).
Address for reprint requests and other correspondence: H. Lum, Dept. of Pharmacology, Rush Presbyterian St. Luke's Medical Center, 2242 W. Harrison St., Suite 260, Chicago, IL 60612 (E-mail:hlum{at}rush.edu).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2002 the American Physiological Society


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Texans RG Jeff Allen is recovering from offseason ankle surgery.
Texans RG Jeff Allen suffered a concussion in Week 14 against the Colts.
Texans RG Jeff Allen (concussion) was ruled out for Week 6 against the Colts.
Texans RG Jeff Allen exited Week 5 against the Vikings with a concussion.
Texans signed G/T Jeff Allen, formerly of the Chiefs, to a four-year, $28 million contract.
FOX Sports' Alex Marvez reports the Bears and Falcons are "heavily pursuing" free agent G/T Jeff Allen.
Eagles VP of football operations Howie Roseman said fixing the offensive line is "a priority, period."
The Chiefs believe RT Jeff Allen only has an MCL sprain.
Chiefs RT Jeff Allen suffered a knee injury in Saturday's preseason opener.
Chiefs OL Jeff Allen (elbow surgery) is 100 percent healthy.
Chiefs placed LG Jeff Allen (elbow surgery) on injured reserve, ending his season.
Chiefs LG Jeff Allen needs elbow surgery and is likely done for the season.
Chiefs LG Jeff Allen and RT Donald Stephenson worked with ex-NFL C LeCharles Bentley during the offseason.
Chiefs LG Jeff Allen was forced from Thursday's game against the Eagles with a right groin injury, and is doubtful to return.


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THIS RESTAURANT IS CLOSED 118 Degrees Send to Phone
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Text data on THIS RESTAURANT IS CLOSED 118 Degrees to your phone, or a friend's cell phone.
Find out which restaurants and chefs top the list of GAYOT's 2016 Annual Restaurant Awards.


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If not, do you feel like you might be excluding someone great?
I have started seeing a guy who is completely not my type. He is 35 but looks much older- he is also very quiet and reserved, but he calls and checks on me daily. I like guys who talk a lot and who are active (do things outside like walking etc.).
I feel I am seeing him only because he cares for me, not really because I like him.
Any advice?
Most Helpful Girl
analyze yourself and whether or not you can see yourself pursuing anything with this guy. if you don't see yourself with him in a couple months, then i suggest you end it to lessen his pain when you shut off his advanced.10|10|0


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19 August 2009
Evolution 2.0: On the origin of technologies
BARELY four years after the publication of Darwin's On the Origin of Species, the Victorian novelist Samuel Butler was calling for a theory of evolution for machines. Since then, a few hardy souls have attempted to oblige him, but none has quite hit the mark. Their reasoning, very much à la Darwin, is that any given technology has many designers with different ideas – which produces many variations. Of these variations, some are selected for their superior performance and pass on their small differences to future designs. The steady accumulation of such differences gives rise to novel technologies and the result is evolution.
This sounds plausible, and it works for already existing technologies – certainly the helicopter and the cellphone progress by variation and selection of better designs. But it doesn't explain the origin of radically novel technologies, the equivalent of novel species in biology. The jet engine, for example, does not arise from the steady accumulation of changes in the piston engine, nor does the computer emerge from accumulated changes in electromechanical calculators. Darwin's mechanism does not apply to technology.
So what would a theory of evolution for technology look like? Do technologies descend by some unambiguous process from the collective of earlier technologies? In my new book, The Nature of Technology, I argue that they do. But to see how, we need to tailor our thinking directly to technology, not borrow from biology.
To start with, we can observe that all technologies have a purpose; all solve some problem. They can only do this by making use of what already exists in the world. That is, they put together existing operations,


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1. Was Pam Puckett actully going to the hospital when Sam and Melaine were born on the bus? 2. Did Freddie have glasses when he was younger and just got contacts as he got older?
3. Were Sam and Freddie REALLY good friends before Carly moved to Seattle and Sam just stared picking on Freddie because Freddie got a crush on Carly?
4. Is Carly's mother in the navy like Carly's dad?
Well thats about it. I hope you like and plz commet what you think the answers are.
PS For #3 i dont want creddiers getting mad at me but this wondering is based on my seddie shipping.


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VCRC Recruitment 2016 Project Assistant
Indian Council of Medical Research, Vector Control Research Centre (VCRC), `Pondicherry has issued a Notification for the Recruitment of 03 Project Assistant posts on Temporary & adhoc basis. The Eligible candidates may apply through the prescribed application format on or before 18th May 2016.
Important Dates:
Last date for Submission of Application 18th May 2016
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Project Assistant (03) posts
- Surat (Gujarat) 01
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The Eligible candidates may apply through the prescribed application format Self Attested copies of certificate in proof of Date of birth, Educational Qualification (Certificate/ Statement of marks), Experience as mentioned under essential qualification and Community certificate for the posts can download the application from the VCRC website (www.vcrc.res.in) and submit the same on or before 18.05.2016 to the Director, Vector Control Research Centre, Medical Complex, Indira Nagar, Puducherry-605 006.
Download Official Notification File
Visit Official Website
Download Application Surat (Gujarat)
Download Application Nalgonda (Telengana)
Download Application Muzaffarpur (Bihar)


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New York Mets fans finally have something to be excited about in 22-year-old starting pitcher Zack Wheeler. Wheeler was acquired from the San Francisco Giants at the 2011 trade deadline in exchange for Carlos Beltran. Baseball America ranked Wheeler as the 35th best prospect at the beginning of the 2012 season. After posting a 3.26 ERA and a strikeout per inning in a season split between AA and AAA, Wheeler is primed to reach the top-20.
His next accomplishment will come when Wheeler makes his major league debut during the 2013 baseball season. Mets fans and fantasy baseball owners are wondering when his debut will happen and how good he can be.
The answer to the first question is simple but not what fans are hoping to hear. Those that believe Wheeler will head north with the ballclub after spring training are either naïve or don't know the Mets very well. The scrutiny over management's notorious mishandling of prospects has caused the newest regime to be overly careful. The only thing that Mets management has been more careful with than young players is money.
These two factors guarantee that Wheeler will open the season in Las Vegas unless he has a lights out spring. If Wheeler is promoted before the all-star break, he could qualify for Super-Two arbitration. This means that Wheeler could apply for arbitration after two years instead of the usual three if he is on the MLB roster for more than 86 games of his rookie season and ranks in the top 22% of players between two and three years of service. It's confusing and frustrating to fans but management projects that Wheeler will cash in on arbitration and wants to delay it as long as possible. They will use the concern over his control as an excuse to have him start the season in AAA.
Wheeler did walk 16 batters in 33 innings at AAA last season and has been guilty of erratic over his young career. While he will never be Greg Maddux, Wheeler will improve his control enough be considered wildly effective at the major league level. With his arsenal of pitches, he will get enough swings and misses that he won't need to beg for called strikes.
Wheeler features an explosive fastball that comfortably sits in the mid-90's and can top off as high as 97. Equally impressive are Wheeler's breaking pitches, which include a plus curveball and slider. When he is using all of his pitches accurately and effectively, Wheeler can be untouchable. Wheeler was dominant at times during the 2012 season, striking out seven or more batters in 11 of his 25 starts and allowing two runs or less in 18 of them.
Apart from drastic or unforeseen circumstances, you can expect Wheeler to make his major league debut shortly after the all-star break. He will arrive in New York with even more excitement and anticipation than his teammate Matt Harvey did during the 2012 season.
He should not be drafted in fantasy leagues but his status should be monitored closely over the first half of the season. He has a chance to be the best rookie pitcher in the MLB once he gets the call.


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Sancta Maria Hospital has appointed MGB PR to manage its PR, social media and marketing communications as they work to raise awareness of the private hospital's facilities, charity work and customer care in South West Wales.
Natasha Fulford, Managing Director of MGB PR said: "Here at MGB PR, we have a wealth of experience working with companies of all sizes, to achieve their PR and marketing goals so we are thrilled to be working with Sancta Maria Hospital. We already have a number of exciting projects lined up and the MGB team and myself look forward to working with them on some very interesting campaigns in the future."
Stuart Hammond, Hospital Director at Sancta Maria Hospital, said: "MGB PR blew us away with their creative ideas and enthusiasm. They understood our commercial needs as well as the importance of putting our customers first in all of our activity. As we grow, we are offering more and more services, with new sports rehabilitation and cosmetic surgery facilities as well as our more traditional GP and surgery services so appointing MGB to help us ensure our customers are up to date with what we can offer seemed like a natural progression. We look forward to working with them while our services continue to grow."


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WAN optimization appliances improve use of existing bandwidth by transparently reducing the amount of traffic pumped into the WAN. They smooth over TCP shortcomings via better window management, for example. They optimize chatty upper-layer LAN protocols like MAPI, HTTP, and Windows file sharing.
We surveyed 486 IT professionals who use or have evaluated WAN optimization appliances to find out how they perform. Not surprisingly, Cisco Systems gets invited to the WAN optimization party by nearly half (49%) of respondents, largely because of its ubiquity in enterprise data centers. Riverbed is a close second, with 40%, handily beating out the rest of our field. Citrix, Juniper, Blue Coat, and F5 round out the top six.
Blue Coat, Cisco, and Riverbed have the highest rankings based on 10 evaluation criteria, including product performance and reliability (see chart).
While Blue Coat and Riverbed lead on performance in our survey, you generally won't find much variation in raw deduplication rates among products. Where you will find differentiation is in how much data the appliances can process on the LAN side. Vendors tend to scale their appliances based on WAN capacity, from T1 speeds to 1 Gbps and beyond. This scaling doesn't reflect network performance as much as the size of the internal storage array containing the deduplication data set, the number and types of CPUs, and RAM. The larger the WAN pipe and the more capacity that's consumed, the larger the disks must be to build an adequate dictionary. And, of course, adequate CPU and RAM are needed to be responsive.
Automatic application detection and detection within HTTP are the WAN optimization features that are particularly important as companies "webify" more applications and adopt VDI. Blue Coat and Citrix have long touted their automated application discovery features that can poke into oft-used protocols, such as HTTP, and differentiate Outlook Web Access from PeopleSoft and act accordingly. Citrix, in its quest to move everyone to VDI, has a leg up on optimizing its own popular VDI protocols.
Not to be left out, other WAN optimization vendors say their products can perform some VDI optimization as well, though they're usually limited to TCP optimizing and packet coalescing. While many optimizers offer manual configuration, where administrators specify the server IP address and other details, having built-in detection means one less task set added to your change-control processes. This is a key distinction to look for when shopping for a WAN optimization product.
Most respondents using WAN optimization are happy with their appliances and aren't actively looking to replace them. Still, vendors shouldn't become complacent. Respondents say advances in technology and a more cost-effective offering could spur them to kick out an incumbent.
User Rank: Apprentice
Mon, 10/17/2011 - 11:22
Silver Peak beats em all, read more here http://www.informationweek.com... and here http://www.informationweek.com....
User Rank: Apprentice
Mon, 12/02/2013 - 06:23
Bridgeworks is pretty good if you're looking for a smaller company that will take better care of you - http://www.4bridgeworks.com
User Rank: Apprentice
Tue, 10/18/2011 - 06:38
Consider Exinda (www.exinda.com) for a WAN Optimization 2.0 approach that looks beyond blind acceleration by intelligently accelerating based upon dynamic policies that allow you to tie your bandwidth consumption to business imperiitives. In June Exinda launched a single sided cache that improves access to cloud based services and also reduces the impact of video and other internet objects on WAN bandwidth consumption.
User Rank: Apprentice
Tue, 11/15/2011 - 02:42
Dear Mike, thanks for the interesting survey - I found remakable that all solutions finally look identical at a few % - They are all good products from great vendors - to satisfy pure WAN optimization / compression requirements, there is no reason to look ahead. This being said, in our new world of cloud computing and unified communications, there are a lot additional challenges in enterprise networking - May I suggest a look to a very different approach (WAN governance through Autonomic Networking) from Ipanema Technologies, that proved its efficiency every day on 150,000+ branches (most of them in Europe but entering now in N/A)? With best regards - Thierry Grenot - CTO - www.ipanematech.com www.wan-governance.com


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- Academic Intelligence24%(8)31%(16)Vote44%(8)
- Emotional Intelligence76%(26)69%(36)Vote56%(10)
Most Helpful Girl
Emotional intelligence, plus social and communication skills.
Academic intelligence is good, but being able to understand and interact well with other people usually does more to help a person succeed in life and relationships. Making great connections with people and creating opportunities for yourself will typically be of more use to you than whether you got an A or B in Statistics sophomore year.10|10|0
Hi Star. :-)
Hey! :)
Most Helpful Guy
Educational intelligence can be taught MUCH more easily, so I feel emotional intelligence is more important.10|10|0
Thanks for the vote. =)


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According to media reports, actor-politician Babu Mohan said to have indulged in an argument with police officials at an event in Jala Vihar on Saturday. It all started when cops asked Babu Mohan's driver to keep his car outside as there was no sufficient place.
Taken aback by this gesture of police, Babu Mohan resorted to an argument which created a mild ruckus at the venue. However, the issue was solved later amicably. Babu Mohan apparently expressed his displeasure over cops functioning and said, "I'm being treated badly deliberately." The former minister in TDP's goverment has shifted his loyalties and joined TRS ahead of elections. Babu Mohan is now representing Andhol constituency in Medak district. He has won over ex Deputy CM Damodar Rajanarsimha with a thumping majority of over 40,000 votes. Babu Mohan was said to be disappointed as he was not taken into KCR's cabinet.


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Robert has jumped in with both feet. He made his first jerk-off video at Sean Cody a few weeks ago, and before he left his first session he inquired about how a guy would do more than jerk off. Sean Cody is always happy to accommodate the curious straight guys, so he set Robert up with another hot stud, and this horny Latino got his ass pounded for the first time.
Robert got his ass fucked twice more before he finally buried his bone in another guy's ass. But it looks like Robert has discovered that he likes bottoming more than topping, because he's back again and getting his ass dicked by Stuart, a very hairy stud.
Robert was very interested in Stuart's hairy body and started exploring it. Robert and Stuart playfully devour each other and swap blowjobs. Robert's got a pretty big cock, so Stuart really had to stretch to slide Robert's big meat down his throat. Then Stuart climbs on top of this cute Latino and starts fucking his ass. I loved this part the best because we really get a good look at Stuart's very hairy legs and butt.
By the time this ass fucking is over, the sheets are covered in sweat and spunk, and these two boys are left a soaking and heaving mass, nuzzling in each other's arms and trying to catch their breath. Robert and his tight Latino ass are featured in five hardcore videos at Sean Cody, and you'll want to explore them all.


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Luggage is very important to those who are frequent travelers and having a compact carry-on bag is a must. Presenting the Louis Vuitton Icare, a bag that is suitable just as much for traveling as for the daily professional. It was created by Louis Vuitton in honor of his father and named with reference to Icarus, a character in Greek mythology who is the son of Daedalus and is commonly known for his attempt to escape Crete by flight, which ended in a fall to his death.
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Image via Louis Vuitton


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I asked a girl out on a first date and it went really well we laughed a lot. when she went out of the car she said we will meet next week. I asked her out for a second date she accepted. I texted her 2 days later to set the the time. the second date was longer and we laughed a lot. during the movie she was initiating conversation she asked me if ithink this actress is pretty and I told her she is not my type then she was like whats your type then.
at the end I dropped her home. she said thank you so much for everything the dinner and the movie. she didn't mention meeting again which made feel like she may be lost interest although we had lots of fun
I send her a text today 7 hours ago I asked her out for next week. I didn't get any reply so is it desperate if I call her or should I wait?
Most Helpful Girl
Call her tomorrow. If she doesn't get back to you, then leave it at that.10|10|0
this happened on the first date when I texted her about the time. she didn't reply to the message for 6 hours and when I called she replied instantly and she was like happy that called. she didn't have credit back then.
Most Helpful Guy
Wait til a later day. Just start up a normal conversation with her later without bringing up another date and see how she responds21|10|0