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One of the many signs that we have lost control of our destiny is that Big Brother is watching everywhere we go. After all, the CIA was allowed to listen in on our telephone conversations without authority or penalty because of the alleged weapons of destruction they were monitoring. While there are situations such as this that we have no control over, we need to be aware of monitoring we may be subjecting ourselves to voluntarily.
The latest invasion of personal privacy is being done by Sears. If you decided to sign up for this retailer's emails and you joined the "My SHC Community†you are probably sharing a lot more information about yourself with others than you thought. Apparently, you are not just sharing your details with Sears but with comScore, a company that tracks and aggregates browsing habits on the Internet.
When you install the Sears software, you also install VoiceFive software that provides data directly to comScore about your Internet browsing habits, basically making it spyware. Clearly this program is working for comScore because they have been able to effectively monitor people's browsing habits – most likely, unknown to most of the folks being watched. In fact, comScore is the company that provided the stats showing that GodTube was the quickly growing website last year and that more people stole Radiohead's most recent album than those who legally downloaded it. Would you reveal this type of stuff knowingly to a spyware company? I sincerely doubt it but obviously comScore has been able to quietly monitor and compile browsing habits for millions of people to come up with these kind of statistics.
However, Sears is legally correct in their actions because they did make mention of the software deep within the privacy statement users need to agree to prior to signing up for the SHC Community. The privacy statement, in part, reads:
Once you install our application, it monitors all of the Internet behavior that occurs on the computer on which you install the application, including both your normal web browsing and the activity that you undertake during secure sessions, such as filling a shopping basket, completing an application form or checking your online accounts, which may include personal financial or health information.
Once again, if this was more clearly stated in the forefront of Sears' website, I doubt that many people would sign up for this "communityâ€. Would you knowingly allow unknown parties to check out everyone you go online, including you personal bank accounts, credit accounts and health information? However, it is scary to think that you've already allowed someone this access if you joined Sears SHC Community.
Certain experts feel that this disclosure was too hidden to meet the intentions of the FTC regulations requiring companies to make it clearly apparent when spyware was going to be used. Somehow I don't believe that sticking the statement above in a multiple page privacy statement documents is clarity, but it won't be Sears that ultimately pays for this invasion of privacy – it will be the individuals who unwittingly supplied their personal details and continued to surf the Internet while they were being watching by spyware.
Sears is just a small example of what is going on internationally with regard to invasion of privacy and misrepresentation. Big business and government continues to get away with exploiting our basic rights so we need to become knowledgeable on our own and read all the fine print to protect ourselves in this predatory world. Mamasaid there'd be days like this…


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The World's Longest-Running Trademark Dispute
Jay Parkhill December 18th, 2008
I don't know for a fact that the fight between Anheuser-Busch and Budejovicky Budvar NP over the marks "Budweiser" and "Bud" is officially the oldest continually active trademark dispute in the world (Erik?), but it must be close.
The Budvar brewery was founded in 1895. The beer was named for the Germanized version of the city in which it was founded, Budweis (České Budějovice in Czech). Distribution was extremely limited until the second half of the twentieth century. Anheuser-Busch was founded in the 1850s and introduced the Budweiser brand in 1876.
For decades the two brands existed in parallel, using very similar names and script for their logos, but not directly competing on a large scale. That changed when Anheuser started selling Budweiser in Europe in earnest and the two companies have been playing a real-world version of the game Risk ever since, locking up trademark rights country by country. Budvar obtained first rights in Czechoslovakia, France and Austria and Anheuser had primary rights in the US.
Anheuser won in Australia, New Zealand and 23 or 27 EU member states, among others. Budvar won in Japan, but seems to be losing the war. This week, though, a European Union court denied Anheuser's move to register the Budweiser mark across the EU.
It may not be much more than a symbolic victory for Budvar. One has to think that the cost of litigation is a much bigger issue for tiny Budvar than for giant Anheuser, now owned by mega-giant InBev. The multi-jurisdiction chess match is fascinating to watch, though.
[Full disclosure: I once carried a six pack of Czech Budweiser home from Prague]Tags: beer, trademark

subset: commoncrawl


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A high-throughput chemically induced inflammation assay in zebrafish
Studies on innate immunity have benefited from the introduction of zebrafish as a model system. Transgenic fish expressing fluorescent proteins in leukocyte populations allow direct, quantitative visualization of an inflammatory response
Here we show that specific, noninvasive damage to lateral line neuromast cells can induce a robust acute inflammatory response. Exposure of fish larvae to sublethal concentrations of copper sulfate selectively damages the sensory hair cell population inducing infiltration of leukocytes to neuromasts within 20 minutes. Inflammation can be assayed in real time using transgenic fish expressing fluorescent proteins in leukocytes or by histochemical assays in fixed larvae. We demonstrate the usefulness of this method for chemical and genetic screens to detect the effect of immunomodulatory compounds and mutations affecting the leukocyte response. Moreover, we transformed the assay into a high-throughput screening method by using a customized automated imaging and processing system that quantifies the magnitude of the inflammatory reaction.
This approach allows rapid screening of thousands of compounds or mutagenized zebrafish for effects on inflammation and enables the identification of novel players in the regulation of innate immunity and potential lead compounds toward new immunomodulatory therapies. We have called this method the chemically induced inflammation assay, or ChIn assay.
See Commentary article:
Inflammation is a reaction of the immune system to tissue damage and infection and represents a key component in normal tissue homeostasis. Consequently, deregulated inflammatory reactions result in severely detrimental chronic conditions. One of the hallmarks of the innate inflammatory response is infiltration of the affected tissue by leukocytes of the innate immune system (that is, granulocytes and macrophages). Inflammatory cells are recruited to the site of wounding or infection by proinflammatory mediators such as hydrogen peroxide, cytokines and chemokines
We have been studying the induction of cell death and regeneration in neuromasts of the lateral line system of zebrafish larvae. Neuromasts, small clusters of mechanosensory hair cells enclosed within a compact group of accessory cells, are regularly distributed over the body surface and can be damaged by exposure to physical or chemical insults
Myeloid leukocytes migrate to damaged neuromasts
Zebrafish larvae establish the primary lateral line system by 3 days postfertilization (dpf). Addition of copper sulfate to the water rapidly destroys hair cells of the lateral line system by inducing oxidative stress followed by cell death
Additional file 1
Supplementary Figure 1. Induction of
Click here for file
To visualize the presence of leukocytes of the innate immune system and to follow their behavior
We exposed transgenic zebrafish larvae at 56 hours postfertilization (hpf) to 10 μM CuSO4 for a period of 2 hours and monitored the behavior of fluorescent leukocytes immediately after beginning the treatment. In control (untreated) fish, most of the immune cells remained in the PBI or CHT (Figures
Figure 1
Leukocytes migrate specifically to damaged lateral line neuromasts in zebrafish larvae
Leukocytes migrate specifically to damaged lateral line neuromasts in zebrafish larvae. (a-j) 56-hours postfertilization (56-hpf)
To examine the dynamics of the inflammatory process under these conditions, we captured time-lapse images of the trunk and tail of
Additional file 2
Supplementary Movie 1. Migration of leukocytes toward damaged neuromasts. Transgenic
Click here for file
Neuromasts consist of centrally located hair cells surrounded by mantle cells on the surface and supporting cells at the base, all forming a compact rosette
Additional file 3
Supplementary Movie 2. Leukocytes patrol among neuromast cells after copper induced damage. Description: Compound
Click here for file
The two transgenic lines used in this study label myeloid leukocytes, most likely neutrophils. However, up to 48 hpf, the
Additional file 4
Supplementary Figure 2. Neutrophils and macrophages behave similarly in response to copper exposure. Compound
Click here for file
To determine whether copper-induced damage in other tissues also stimulated an inflammatory response, we exposed
Additional file 5
Supplementary Figure 3. Behavior of leukocytes after long-term copper exposure in zebrafish larvae. At 3 days postfertilization (dpf), transgenic
Click here for file
Quantification of immune cell infiltration in the lateral line after chemical damage
Taking advantage of our findings, we aimed to develop a simple but robust quantitative method to measure the degree of leukocyte infiltration in damaged neuromasts. Using such a protocol would allow us to reveal the effect of molecules that modulate the inflammatory response elicited by copper exposure, opening the door for chemical or genetic high-throughput inflammation screens. Our first approach (manual quantification) requires only visual inspection of treated and control larvae and can thus be carried out using low-power magnification. It is necessary to have a method for detection of innate immune cells; the optimal way is to use transgenic lines such as
Additional file 6
Supplementary Figure 4. Chemically induced inflammation assay (ChIn) using Sudan Black. (a and b) Bright-field images of untreated (a) and 10 μM CuSO4-treated (b) 56-hpf
Click here for file
Figure 2
Quantification of infiltrating leukocytes in the lateral line after diverse treatments: the chemically induced inflammation (ChIn) assay
Quantification of infiltrating leukocytes in the lateral line after diverse treatments: the chemically induced inflammation (ChIn) assay. (a) Schematic view of a 3 days postfertilization (dpf) larva. The boxed area corresponds to the horizontal myoseptum (line). An area of approximately 10 cell diameters is delimited around the myoseptum (dotted red lines) and corresponds to the area where leukocytes were counted in all manual quantification experiments. (b) Significant induction of leukocyte recruitment to the lateral line by copper treatment. The graph shows average leukocyte numbers in the lateral line in negative controls (untreated fish or cadmium chloride-treated fish) and in copper-treated fish. (c) The effect of copper on leukocyte recruitment to the lateral line is concentration dependent. (d) Effectiveness of other metals in the ChIn assay. (e) Neomycin, at concentrations that eliminate hair cells, also induces leukocyte recruitment, but less effectively than copper. (f) Larvae of different ages, from 56 to 128 hpf, were exposed to 10 μM CuSO4 and were monitored for leukocytes present at the myoseptum every 20 minutes thereafter until 120 minutes. Fish at all stages analyzed showed similar behaviors and exhibited increased presence of leukocytes at the lateral line by 20 minutes after initiating exposure to copper. For all experiments, at least 15 larvae were used for each condition. ***
Additional file 7
Supplementary Figure 5. Neomycin ablates hair cells but fails to induce localization of leukocytes to the horizontal myoseptum. (a and c) Transgenic
Click here for file
As treatment with 10 μM copper for up to 2 hours does not affect the viability of the larvae, we used this concentration of metal as the inflammation-inducing dose for all subsequent experiments. To determine the optimal developmental stage at which to carry out the inflammation assays and the times at which to obtain the best response after treatment, we exposed fish of different ages (56 hpf to 128 hpf) and performed immune cell counts at the myoseptum between 20 and 120 minutes after the addition of copper. All ages tested showed comparable response curves, whereas significant infiltration of the lateral line by leukocytes was always detected by 20-40 minutes after initiating copper treatment (Figure
Effect of anti-inflammatory drugs and inhibition of reactive oxygen species
Previous reports have shown that well-established anti-inflammatory drugs behave as predicted in zebrafish inflammation assays that rely on physical wounding
Table 1
Detection of anti-inflammatory activity using the ChIN assaya
Mode of action
Significant effect
COX inhibitor
COX inhibitor
JNK inhibitor
COX-1 inhibitor
Mifepristone (RU486)
Progesterone and GR antagonist
Steroidal nitric oxide synthase inhibitor
COX inhibitor
PPAR-γ agonist
COX inhibitor
Steroidal GR agonist
NS COX-1 inhibitor
aSelected drugs were added to the incubation medium 1 hour prior to addition of copper and were tested at the indicated concentrations for inhibition of leukocyte migration using
Figure 3
Proof of principle of the ChIn assay using known anti-inflammatory drugs
Proof of principle of the ChIn assay using known anti-inflammatory drugs. The ChIn assays were carried out by manually counting leukocytes recruited to the lateral line after copper treatment (10 μM) as described in Results. In this experiment, dimethyl sulfoxide (DMSO) was included in all samples, and drugs were added 1 hour prior to copper treatment at the indicated concentrations. As before, experiments were carried out with 15 larvae per condition. ***
To further assess the benefit of the ChIn assay for detecting novel mechanisms guiding inflammation, we were curious whether we would be able to identify the recently described role of reactive oxygen species (ROS) in this process. Formation of a gradient of H2O2 has been shown to be required for leukocyte recruitment toward wounds inflicted by tailfin transection in zebrafish
Figure 4
Detection of H2O2 inhibition and genetic mutations using ChIn assays
Detection of H2O2 inhibition and genetic mutations using ChIn assays. (a) Reactive oxygen species signaling is critical for copper-induced inflammation. Diphenyleneiodonium (DPI), an inhibitor of NADPH oxidases, impairs wounds to leukocyte signaling, resulting in reduced leukocyte numbers at damaged neuromasts. Larvae at age 56 hpf were treated with 100 μM DPI for 1 hour prior to copper treatment (10 μM CuSO4 for 40 minutes). DPI treatment results in significantly lower leukocyte numbers within the myoseptum compared to untreated control larvae. (b) The ChIn assay enables detection of genetic mutations affecting an inflammatory response. Clutches from matings between homozygous
As innate immune leukocytes abandon the wounded neuromast a few hours after the response, we next wished to learn whether it would be possible to also detect molecules that affected the resolution phase. Nonsteroidal anti-inflammatory drugs (such as diclofenac and ibuprofen) act as nonselective inhibitors of the enzyme cyclooxygenase (COX), inhibiting both the cyclooxygenase-1 and cyclooxygenase-2 isoenzymes (COX1 and COX2, respectively). COX enzymes are proinflammatory during the early phase of inflammation, but aid resolution at the later phase by generating an alternative set of prostaglandins
Additional file 8
Supplementary Figure 6. The ChIn assay can be adapted to carry out an inflammation resolution screen.
Click here for file
The ChIn assay can detect mutations that affect the inflammatory response
To explore the full versatility of the ChIn assay, we investigated its potential for detecting genetic mutations within critical pathways of an innate immune response. To this end, we used the zebrafish
Automated detection of anti-inflammatory activity
While manual quantification of migrating leukocytes using the ChIn assay is an improvement over other methods for analysis of selected molecules or for recovering mutations, it is still unrealistic as a method for high-throughput screening of hundreds to thousands of candidate chemicals or mutants with reasonable efforts and within reasonable time. Therefore, we sought to transform the ChIn assay into a scalable method that could be used in large-format screens. We developed a custom software script enabling us to map fluorescent expression domains in zebrafish larvae and combined it with automated microscopy. To put the automated system into operation, we used compound transgenic
Figure 5
Automated ChIn assay
Automated ChIn assay. (a-d) Image acquisition method using compound transgenic larvae
We describe a new method, the ChIn assay, which will be of value for the initial detection of lead compounds exhibiting immunomodulatory activity as well as genes with roles in the different stages of the inflammatory response. In zebrafish, myeloid leukocytes develop during the first day of life, while molecular markers and cellular components of adaptive immunity arise well after the third day
The inflammatory response in this species has been well characterized
We applied several known anti-inflammatory drugs to test the ChIn assay. The selected molecules affect diverse pathways implicated in inflammation such as the COX, c-Jun N-terminal kinase (JNK) or peroxisome proliferator-activated receptor (PPAR)-γ responses. In addition, we confirmed that ROS gradient formation is critical for leukocyte recruitment upon copper-induced inflammation as chemical inhibition of NADPH oxidase significantly decreased the leukocyte recruitment toward neuromasts. Copper-mediated inflammation thus mechanistically recapitulates classical wounding assays without the need for manual manipulation of larvae. We caution that a primary large-scale, small-molecule screen carried out using the ChIn assay would not distinguish (1) a specific anti-inflammatory effect, (2) an inhibitory effect on general cell motility and (3) protection of hair cells from undergoing cell death, as all of these events would result in the same net effect: Recruitment of leukocytes to neuromasts would be altered. Candidate leads need to be subjected to secondary screens to distinguish the specific aspect of immune cell behavior that is affected.
Using the ChIn assay, we have obtained comparable results with two transgenic lines and two histochemical stains (Sudan Black and 3,3'-diaminobenzidine, or DAB). New tools currently under development that will allow the
Other types of screens feasible with the ChIn assay
A major application of the ChIn assay will be the identification of immunomodulatory activities of small molecules. However, we foresee additional applications using this strategy. First, mutant screens aimed at detecting genetic components of the inflammatory response will be facilitated with this approach. The high-throughput advantage of the ChIn assay can be used for efficiently screening mutagenized fish, as well as for gain of function screens or antisense screens using morpholino oligonucleotides. Genetic mutations or knockdowns can be identified, given that their phenotypic consequence regarding leukocyte behavior during inflammation lies within the sensitivity threshold of the ChIn assay. Here we provide proof of principle for the identification of mutations using
Second, the localized damage induced in the superficial neuromasts could be used as a model for inflammation mediated by infection. Most models of bacterial infection, for example, require injection of the pathogens to study the immune response [
The highly specific migration of leukocytes to the neuromasts offers an excellent opportunity for a detailed examination of the molecular players involved in directed cell migration and immune cell homing. In this case, immune cells can be imaged
Our laboratory is particularly interested in the regeneration of lateral line hair cells, a model for sensory regeneration in general. The contribution of the immune system to regeneration has been well documented, as has the potential impediment to regeneration caused by prolonged tissue inflammation and fibrosis. We believe that the ChIn assay can be used to investigate the molecular mechanisms of immune system involvement in regeneration. Whether the arrival of immune cells to damaged neuromasts promotes or inhibits regeneration of sensory hair cells is currently under study. Interestingly, time-lapse observation of a neuromast during copper-induced damage shows that patrolling neutrophils and macrophages (and possibly other cell types) provoke disorganization of the surviving cells, separating them from one another (Additional file
In conclusion, the ChIn assay represents a new tool that will contribute to the understanding of the pathways that lead to homing and migration of innate immune cells, as well as providing a discovery model for molecules that may yield new leads to therapeutic treatment of immune disorders.
Zebrafish were maintained and raised in our facility under recommended conditions
A 10 mM stock solution of CuSO4 (copper II sulfate pentahydrate, catalog no. 102780; Merck, Darmstadt, Germany) was prepared daily in bidistilled water in a glass beaker until dissolved completely. Likewise, CdCl2 (CB236; Matheson, Coleman & Bell, Cincinnati, OH, USA) was prepared at a stock concentration of 10 mM. Additional chemicals were ZnSO4 (catalog no. ZI-1705; Winkler S.A., Santiago, Chile), NiSO4 (72280; Sigma) and AgNO3 (101512; Merck), neomycin (N1876; Sigma), 3,3'-dihexyloxacarbocyanine iodide, or DiOC6(3), was purchased from Interchim, Montluçon, France (FP-46764A) or AnaSpec, Fremont, CA, USA (catalog number 84715), DPI (D2926; Sigma), DMSO (317275; Merck), paraformaldehyde (catalog no. 1.04005.1000; Merck), Sudan Black (380B; Sigma), and Tween 20 (P5927; Sigma).
The following anti-inflammatory drugs were tested: from Cayman Chemical Co. (Ann Arbor, MI, USA), ibuprofen (70280), diclofenac (70680), aspirin (70260), indomethacin (70270),
Neuromast damage protocol and the basic ChIn assay (manual quantification)
Zebrafish larvae of the
Selected larvae were transferred to six-well plates (M8562; Sigma) in a volume of 6 ml of E3 solution lacking methylene blue, and 15 larvae were added per well. Stock solutions of CuSO4 were added directly to the wells, and incubation was carried out for 40 minutes at 28°C. Larvae were then fixed by transferring them to 1.5-ml microfuge tubes and replacing the E3 medium with 4% paraformaldehyde prepared in phosphate-buffered saline (PBS) and incubating for 1 hour at room temperature. During fixation and subsequent handling, the tubes were kept in the dark to avoid bleaching or fading of the fluorescent protein signal. After fixation, larvae were washed three times for 5 minutes each in PBS-Tween20 with gentle agitation. Examination of fluorescent cells and counting was carried out within the next 48 hours after fixation using a Leica (Wetzlar, Germany) MZ-12 fluorescent stereoscope. Labeled cells were counted under fluorescent illumination within 10 cell diameters of the horizontal myoseptum between the first somite and the end of the tail (see Figure
For Sudan Black staining, we used fish of the
Drug assays
All drugs were prepared according to the manufacturer's instructions at a stock concentration of 10 mM by dissolving in 100% DMSO, which was previously purged by gaseous nitrogen for 2 minutes. Drugs were stored at -20°C until use and were diluted immediately prior to being added to larvae medium.
Drugs were added to the required concentration into the wells containing the experimental larvae in E3 containing 1% DMSO. Positive and negative control larvae were incubated only with 1% DMSO. Incubation with drugs was done for 1 hour prior to addition of CuSO4, which was added directly to the wells containing the experimental and positive control larvae. Incubation after copper addition continued for another 40 minutes before fixation in 4% paraformaldehyde for 1 hour at ambient temperature in the dark. Counting of leukocytes was carried out as before.
Statistical treatment
Data are presented as mean values ± SEM. Statistical analysis was performed using GraphPad Prism version 5.00 for Windows software (GraphPad Software, La Jolla, CA, USA). The probability level for statistical significance was
Image processing
For imaging, larvae were anesthetized and mounted in 1.5% low melting point agarose (peqGOLD 35-2099; PEQLAB Biotechnologie, Erlangen, Germany) dissolved in E3. Photographs were taken with a Leica DFC 300-FX camera and Leica SPE confocal microscope (Leica Microsystems, Wetzlar, Germany) and processed with Adobe PhotoShop (San José, CA, USA), Zeiss Axiovision (Carl Zeiss Microimaging GmbH, Jena, Germany) and Image J (version 4.2,
Automated ChIn assay
Individual anesthetized larvae were manually placed in single wells of a 384-well plate in embryo buffer. Subsequently, compound stock solution in DMSO was transferred from a compound source plate to the assay plate containing embryo buffer (E3 + 1% DMSO + 0.02% MS222) and larvae using a multichannel pipette. The assay plate containing larvae and compound was sealed and incubated for 30 minutes at 28°C. Following compound incubation, CuSO4 solution (0.3 mM) was added to the assay plate, resulting in a final concentration of 10 μM copper sulfate. Assay plates were then incubated for another 40 minutes at 28°C. This procedure yielded a total volume of 120 μl per well and resulted in the respective final compound screening concentrations for this assay (10 μM ibuprofen, 3 μM diclofenac, 10 μM indomethacin, 1 mM dexamethazone, 300 μM hydrocortisone). A volume of 120 μl was empirically identified as the optimal volume for our automated imaging procedure. To achieve an assay sensitivity comparable to manual analysis, we also defined the minimum number of individual larvae analyzed per condition to be 30. In practice, 24 embryos for one condition were imaged per 384-well plate, but experiments were repeated at least in triplicate such that, on average, 50 larvae were analyzed per condition (see below).
Automatic imaging was performed on Olympus Scan^R high-content screening microscope setups (Olympus Biosystems, Munich, Germany) equipped with a ×2.5 lens objective (plan-apochromatic), an Olympus Biosystems DB-1 digital camera (1,300 × 1,024 pixels), filter cubes for GFP excitation filter, 460-480 nm; emission filter, 495-540 nm; dichromatic mirror, 485 nm) and cyanine 3 (Cy3) (excitation filter, 535-555 nm; emission filter, 570-625 nm; dichromatic mirror, 565 nm), and an ultrastable light source MT-20 xenon lamp. Camera image integration times were fixed (20-ms bright-field microscope, 400-ms GFP, 150-ms Cy3). An object detection autofocus algorithm detected the central focal plane of the first-well larva and was applied for the rest of the plate. Image processing was done using the LabView Vision AI rapid prototyping tool (National Instruments, Munich, Germany). Data management, red-green-blue (or RGB) overlay gallery generation and result display were performed using self-made LabView software modules. These scripts enable the detection of GFP-labeled neuromasts on an extended focus projection of five optical sections (two in each direction from the central focal plane) and define an empirically established surrounding area (see Figure
Authors' contributions
CD and OP performed all of the manual ChIn assays and statistical analysis and prepared the figures. CW and CG did experiments related to automated ChIn and Sudan Black stains on
We thank Catalina Lafourcade, Víctor Guzmán, Nadine Eschen and Sebastian Hoetzel for expert fish care, Florencio Espinoza for technical help and Luisa Pereiro for help with time-lapse imaging. Zebrafish strains were kindly provided by Stephen Renshaw, Phillip Crosier, John Rawls, Darren Gilmour, Paul Martin, Herwig Baier and Leonard Zon. This work was supported by grants to MA from Fondecyt (1070867), FONDAP (15090007), ICM (P06-039F), CORFO-Innova (09MCSS-6705), DFG-Conicyt 075-2009; to CD from UNAB (DI-01-09/1) and Fondecyt (24090004); to UL from Dopaminet (EU FP7 223744); and to CG by a Marie Curie International Reintegration Grant (EU FP7; PIRG07-GA-2010-267552).
Inflammatory cells during wound repair: the good, the bad and the ugly
A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish
Pivotal advance: pharmacological manipulation of inflammation resolution during spontaneously resolving tissue neutrophilia in the zebrafish
Using in vivo zebrafish models to understand the biochemical basis of neutrophilic respiratory disease
A transgenic zebrafish model of neutrophilic inflammation
The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish
Analysis of WASp function during the wound inflammatory response: live-imaging studies in zebrafish larvae
Zebrafish as a model for infectious disease and immune function
Modeling inflammation in the zebrafish: how a fish can help us understand lung disease
Imaging macrophage chemotaxis in vivo: studies of microtubule function in zebrafish wound inflammation
Development of the zebrafish lateral line
Acute copper exposure induces oxidative stress and cell death in lateral line hair cells of zebrafish larvae
Sub-lethal concentrations of waterborne copper are toxic to lateral line neuromasts in zebrafish (
Regeneration in zebrafish lateral line neuromasts: expression of the neural progenitor cell marker sox2 and proliferation-dependent and -independent mechanisms of hair cell renewal
Immunohistochemical localisation of the matrix metalloproteinases MMP-3 and MMP-9 within the airways in asthma
Chemokine signaling mediates self-organizing tissue migration in the zebrafish lateral line
Cell turnover in neuromasts of zebrafish larvae
In vivo interstitial migration of primitive macrophages mediated by JNK-matrix metalloproteinase 13 signaling in response to acute injury
Live imaging of chronic inflammation caused by mutation of zebrafish Hai1
Inflammatory resolution: new opportunities for drug discovery
Genotype-proteotype linkage in the Wiskott-Aldrich syndrome
Mutations of the Wiskott-Aldrich Syndrome Protein (WASP): hotspots, effect on transcription, and translation and phenotype/genotype correlation
Ontogeny of the immune system of fish
Use of the zebrafish system to study primitive and definitive hematopoiesis
Myelopoiesis in the zebrafish,
Characterization of zebrafish larval inflammatory macrophages
Transgenic zebrafish reporter lines reveal conserved Toll-like receptor signaling potential in embryonic myeloid leukocytes and adult immune cell lineages
Real-time observation of
Zebrafish embryos as a model host for the real time analysis of
Drug screening for hearing loss: using the zebrafish lateral line to screen for drugs that prevent and cause hearing loss
Identification of genetic and chemical modulators of zebrafish mechanosensory hair cell death
Transparent adult zebrafish as a tool for in vivo transplantation analysis
A GFP-based genetic screen reveals mutations that disrupt the architecture of the zebrafish retinotectal projection
Stages of embryonic development of the zebrafish
The identification of genes with unique and essential functions in the development of the zebrafish,
Purification, characterization and selective inhibition of human prostaglandin G/H synthase 1 and 2 expressed in the baculovirus system
Purification and characterization of prostaglandin H synthase-2 from sheep placental cotyledons
c-Jun N-terminal kinase is required for metalloproteinase expression and joint destruction in inflammatory arthritis
Cancer chemopreventive activity of resveratrol, a natural product derived from grapes
RU486 (mifepristone): mechanisms of action and clinical uses
Effect of dexamethasone on nitric oxide (NO.) production by cultured astrocytes
The structure-activity relationship between peroxisome proliferator-activated receptor γ agonism and the antihyperglycemic activity of thiazolidinediones
Glucocorticoid signaling and stress-related limbic susceptibility pathway: about receptors, transcription machinery and microRNA
Enhancement of chemotherapeutic drug toxicity to human tumour cells


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Marcel Theroux is a British novelist, journalist and documentary film-maker. He was born in Kampala, Uganda, in 1968, and grew up in Wandsworth, south London. After graduating from Clare College Cambridge, he studied international relations at Yale University, specialising in Soviet and east European studies. He has presented several award-winning documentaries, including Oligart – The Great Russian Art Boom and Unreported World for Channel 4, but is best known for his novels A Stranger in the Earth, The Confessions of Mycroft Holmes and last year's acclaimed Strange Bodies, now in paperback.
Television: Inside No 9
A terrific series of six stand-alone 30-minute comedy drama films, sort of like a modern take on Roald Dahl's Tales of the Unexpected. They are dark, they are weird, they are funny, they are beautifully made and beautifully acted. The conceit is that each takes place in a house numbered 9, every house being on a different street. One is set entirely in a cupboard during a prolonged game of sardines at a garden party. It's like Festen (the Danish social satire) reimagined by the Monty Python crew.
Books: The Disenchanted by Budd Schulberg
This has just been reprinted. Budd Schulberg is best known for writing On the Waterfront, but The Disenchanted is a novel about his time co-writing a crappy, campus-based, comedy-musical with an ageing and impoverished F Scott Fitzgerald in Hollywood in the early 1930s. By that time Fitzgerald was a broken-down alcoholic; his literary career had hit the skids. He and Schulberg were sent to a New Hampshire college to research the book. Fitzgerald was on the wagon but his dad gave them a bottle of champagne and this caused Fitzgerald to go on this epic bender and the whole thing turned into an absolute shambles. He was dead six months to a year later. It's a superb portrait of a great, great artist in tragic decline. Anyone with a true interest in Fitzgerald should read it. It is as close as you will ever get to actually meeting the man.
Leisure: swimming in Tooting Bec lido
I have been swimming there for two years now. It is unheated pool and I swim through the whole year. This winter, as you can imagine, it has been unbelievably cold. Doing it on the freezing days makes you truly appreciate the sunny days. I think Tooting Bec lido is one of the great jewels of London. It is beautiful in itself – this vast expanse of bright blue water in the heart of south London – but its beauty is enhanced by the bizarre collection of people, of which I am proud to say I am one, who swim there every day, no matter what the weather.
Games: Hero Kids
When I was a kid I was a massive Dungeons and Dragons nerd. I recently discovered Hero Kids, which is an online Dungeons and Dragons for small kids, age five and upwards I think. And I play it with my children. I think role-playing games have not been given their due. They really do stretch the creative imagination. A considerable amount of art can probably be attributed to playing Dungeons and Dragons at an early age, not least the art of the contemporary computer game that owes a lot of its conventions and scenarios to Dungeons and Dragons. For my children it is wonderful because you really do see them immersing themselves in the world and the characters.
Place: Crimea
I wanted to mention Crimea as it is beautiful and extraordinary, and is now in the news for pretty awful reasons. Anyway, it had me thinking of the recent horse meat scandal. One of the strange effects was that all of a sudden there was a jump in the demand for horse meat; people read about it and decided they wanted to try it. I am hoping this awful news does something similar for Crimea. I have been there on a number of occasions and have really fallen in love the place. It has huge mountains, great beaches and amazing architecture. Chekhov had a home here. There are also some superb Crimean wines, alongside some real gut rot, but nonetheless I think there is something inherently appealing about Crimean wine. It has incredible Tatar palaces and most famously and mindbogglingly the Vorontsov Palace, which was commissioned by an anglophile and is a classical design heavily modified by an Odessa architect called Francesco Boffo – it's part Scottish baronial, part Mughal. And it sits in these amazing landscaped German gardens and woods. It is a testimony to the extraordinarily diverse cultural influences Ukraine has enjoyed.
Museum: The British Museum
I am a member of the British Museum and it is one of my favourite places in the world – always surprising and spooky. You can wander around it and come across an entire civilisation you had no idea existed, and yet it produced art and had a legal system, and wars and peace. I sometimes wonder if our own civilisation could end up like that, as a sort of museum peace unknown to all but a few academics. It's very humbling, as it emphasises our own insignificance in the grand scheme of things. I am really looking forward to the new Viking exhibition. As a child I read Myths of the Norsemen by Roger Lancelyn Greenand became obsessed with Vikings. As an adult I love Viking sagas. It is said to debunk our view of Vikings as bunch of savage marauders that arrived in their longboats and killed monks. The problem is people always say this about Viking exhibitions, then you go and discover that despite the publicity the Vikings were indeed a bunch of savage marauders who arrived in longboats and killed monks. But I am not going to allow that to put me off. The British Museum is always a joy.


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Who qualifies as a dependent—Can I claim my daughter's significant other and his child as dependents?
To claim your child's significant other, he or she must meet these requirements for a qualifying relative:
- The person must not qualify as somebody else's qualifying child.
- The person must live with you the entire year (365 days) .
- The person must have earned less than $4,050.
- You must provide more than half of the person's support for the year.
Any dependent must not file a joint return unless he or she is only filing to claim a refund. This applies to qualifying relatives and qualifying children. Also, there must be no tax liability for either the dependent or the dependent's spouse if they would have filed separate returns.
You might be able to claim the child of your daughter's significant other. To do so:
- The child must be a considered a qualifying child or relative using the rules above.
- The child must not qualify as the significant other's child. This is true unless your child's significant other isn't required to file a return. So, he wouldn't file a return except to receive a refund of taxes withheld and wouldn't claim the child on that return.
- The child must not qualify as any other taxpayer's qualifying child.
- Both your daughter's significant other and his child must live with you all year.
- You must provide more than half of the support to both the significant other and his child.
- They both must make less than the personal exemption amount of $4,050.
Ready to file?
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So how much will you get (or owe) this year? That's the million-dollar question. We happen to have three very useful calculators to help you estimate your refund or balance due.


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Breast feeding facts
If you are or planning to breastfeed here are some helpful tips. When you start have a lactation consultant help you. If it hurts your baby may not be latched properly, try again, pop your finger in the side of their mouth while they're nursing so you can re-latch them on better make sure the nipple is in by starting with the bottom above their mouth towards their nose. With your opposite arm, pull your baby close to you, tilt their head back slightly and tickle their lips with your nipple until they open their mouth wide.
Lay your baby on his side, with the front of his body facing you; his head, shoulders and hips should be in a straight line and his mouth should be level with, and directly in front of, your nipple. If he is too low, stack another pillow on your lap. You want the baby to come to your breast, rather than leaning over to bring your breast to them.
A fussy baby: If the baby keeps pulling off and on, or wiggling around, he's probably not comfortable. Check that he is on his side, with his shoulders aligned with his hips, and prop him up with an extra pillow if necessary. If he's still fussy, he may need to be burped.
Back pain: If you're feeling back pain, you're likely leaning forward. Sit up straight and reposition your baby so they are level with your breast. You want the baby to come to your breast, rather than leaning over to bring your breast to them. Using a small stool under your feet to lift your knees higher than your hips will also help you sit tall and take the pressure off your lower back.
Common question: How can I tell whether my baby's getting enough breast milk? After a feeding, your baby seems relaxed and satisfied. Your breast should feel softer, less firm. In the first few days, when your baby is getting your thick, valuable colostrum, she may have only one or two wet diapers a day. After your milk comes in, though, your baby will wet six to eight cloth diapers a day, or five or six disposables. (Disposables can hold more liquid than cloth diapers.)
In the first month, your baby has at least three stools a day, and they lighten to a yellowy mustard color by the fifth day after birth. She may have less frequent bowel movements once she's a month old. In fact, it's not uncommon for breastfed babies to skip a day of bowel movements now and then.
For you: Drink plenty of fluids, lots of water, eat a healthy balanced diet, avoid caffeine as much as possible (though a little is okay) be cautious with your medications talk to your doctor. Please don't smoke, for your health and especially theirs.
If you're concerned that your baby isn't getting enough milk, don't hesitate to call your baby's doctor or check in with a nurse or lactation consultant.
If breast-feeding is tougher than you expected, try not to get discouraged. Feeding a newborn every few hours is exhausting, and it's OK to have a slow start. Just remember that the more often you breast-feed your baby, the more milk your breasts will produce — and the more natural breast-feeding is likely to feel.
Yes, yes yes!! You will strain your back, shoulders and neck if you try to move yourself forward. Bring them to you, not the other way around.


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Snively: I don't need an office either
Hillsborough County School Board member Melissa Snively sent this text Friday morning in respose to Thursday's post about Doretha Edgecomb's opposition to the new school board offices:
"Some of us feel the same way. If I need to have a private conversation and avoid a sunshine violation, I find an empty conference room. We have plenty of them all over the building. Plus, if my constituents want to meet with me, I'm not going to make them drive all the way downtown. Schools have meeting rooms too. We all have cell phones and laptops to stay mobile, like we should be."
Once more, setting the record straight on the cost of the new second-floor offices: The district says it will be $40,000 or less. While the five-year plan lists $600,000 for second-floor renovations, those are largely hypothetical and do not have board approval.


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Internal Auditing of Risk Management: Emphasis on effectiveness and compliance to QSR, ISO 13485 and ISO 14971
Product ID: 700272
Training Level: Basic to Intermediate
- Duration: 60 Min
Customer Care
Fax: +1-650-565-8542
Email: firstname.lastname@example.com
This Internal audit training will guide on how to set up an ISO 14971 audit program with specific auditor guidelines.
Internal auditors are being asked to assess Risk Management for effectiveness. This includes assessment of the risk management process as a whole together with auditing key ISO 14971 outputs such as the RM Plan, DFMEA's, PFMEA's and completeness the RM File. The audit must also address risk mangement in post-design transfer areas such as Purchasing / Supplier Management, Environmental Montitoring & Control, Process Validation, Production control, Calibration, etc. Finally, the internal audit is to assess key feedback to/from other key processes such as Complaints and Post-market Feedback, CAPA, Recalls and Advisory Notices, Regulatory Reporting, etc.
Topics covered will include:
- Internal audit requirements according to QSR and ISO 13485: What every internal auditor should know.
- Risk Management and Risk Assessment requirements according to QSR, ISO 13485 and ISO 14971
- Basic implementation of audits and an audit program according to ISO 19011
- Top-down approach to auditing Risk Management as a 'key process'.
- Auditing against ISO 14971: Roles & Responsibilities, Planning, Analysis, Evaluation, Control, Residual Risk Acceptability, RM Report, Production and Post-Production Information
- Auditing against ISO 13485: Examples taken from Purchasing, Environmental Control, Control of NC Product, Calibration.
- Auditing production and post-production feedback: Complaint Handling and CAPA
- Adding value to the organization when performing internal audits of Risk Management or other processes
Who will benefit?:
This webinar will provide valuable assistance to internal auditors, audit managers and personnel dealing with risk management within medical device or IVD companies regulated by the FDA and/or certified to ISO 13485. The employees who will benefit include:
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- Company Executives
- Consultants
Phil Smart is a founding member and Regulatory Consultant with RA/QA International LLC. He serves the US, Asian and European medical device communities as Consultant, Facilitator and Instructor to 21 CFR Part 820, CMDR/CMDCAS, MDD, ISO 13485:2003, ISO 9001:2000, ISO 14971:2000 (Risk Management), ISO 14644, ISO 14698 as well as MHLW/PMDA/Japanese PAL (Ordinances 2, 139, 169, 179)
He has been a European Notified Body Lead Assessor and Monitoring Auditor for over 15 years with auditing experience in a wide range of active and non-active medical device families, risk management for medical devices, electrical safety testing, terminal EtO and Gamma sterilization, sterile packaging, hygiene, environmental controls, calibration and clinical diagnostic laboratories as well as EAC codes 40, 4, 12, 14, 17, 18, 19, 21, 25, 26, 33, 34 and 35.
More Trainings by Expert
International GMP's for Medical Devices: Quality System requirements for USA, Europe, Canada and Japan
Risk Management and Internal Auditing: Effective quality audits with inclusion of ISO 14971:2007
GCP Audit of Clinical Evaluations for Japan: Requirements and expectations from the revised Japanese PAL
4-Region internal audits: Medical Device Quality System audits that cover internal regulatory compliance for FDA, Europe, Canada and Japan
Japan PAL Audits performed abroad by the PMDA: What to expect and how to prepare
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Webinar may be cancelled due to lack of enrolment or unavoidable factors. Registrants will be notified 24hours in advance if a cancellation occurs. Substitutions can happen any time.
If you have any concern about the content of the webinar and not satisfied please contact us at below email or by call mentioning your feedback for resolution of the matter.
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Former American Idol Star Arrested After Fight with Girlfriend
Lifestyle| |
His ex called police from her home in Wyckoff, New Jersey, claiming the star threw her to the ground and kicked her in the groin. Constantine's representative tells TMZ he was acting defensively because she attacked him. The singer is adamant he did not kick her, and claims the argument started after he told her he was leaving her, prompting the woman to throw an object at him, injuring his right thigh.


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text: In Q4, all 3 operating groups grew reported and core sales. Tools, Hardware & Commercial Products had another strong quarter, with reported sales up 7.7% and core sales up 8.0%. Office Products delivered reported sales growth of 3.7%, with core sales up 3.8%. Home & Family delivered reported sales growth of 1.2%, with core sales up 0.9%. In Q4, 10 of our 13 global business units grew core sales, 4 of the 13 grew core sales greater than 5% and 3 grew core sales greater than 10%. Baby & Parenting delivered core sales growth of 4.5% in Q4 and close to 2% in the second half of 2011, as a result of very strong growth of Aprica in Asia and progress towards stabilizing Graco in North America.While there's more work to be done here, this is a step in the right direction. In 2011, our top 14 brands delivered 4.9% reported growth, with the best absolute contributions coming from IRWIN, Lenox, Rubbermaid Commercial and Aprica. Together, these 4 brands grew reported sales over 11%. In the emerging markets, core sales grew over 10% in Q4, with double-digit core sales growth in most of the major emerging market countries. Our Q4 core sales growth was 2.5% in the developed world, with strong growth in Japan and solid results in North America, partially offset by a decline in Western Europe. Our key growth initiatives are yielding the results we expect. Industrial Products & Services achieved their eighth consecutive quarter of double-digit core growth behind strengthened selling capabilities in the emerging markets and the successful launch of our Speed Slot Hole Saw. Parker Ingenuity has been successfully launched into 11 countries now, to positive critical acclaim and terrific sell-through, particularly in Asia and via e-tailers in North America. IRWIN delivered strong growth as a result of our new GrooveLock Pliers and levels and measuring launches. Paper Mate InkJoy has been launched into virtually all countries where Paper Mate is sold, with the bulk of the marketing support still to come. Rubbermaid Medical increased revenues over 80%, as we continue to scale this brand. Sharpie and Paper Mate were successfully launched in Brazil and are making good early progress. And as mentioned, Aprica had a terrific second half in Asia, as virtually every new item we've launched exceeded expectations. Aprica Asia had Q4 core sales growth of over 20%. And Calphalon had an excellent year behind the expansion of kitchen electrics, the new bronze contemporary nonstick line and the distribution win at J.C. Penney.


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Eidur Villa talk rejected
Eidur Gudjohnsen's agent has played down reports linking the Barcelona star with a move to Aston Villa.
By Peter O'Rourke
Last Updated: 11/07/08 11:41am
Eidur Gudjohnsen's agent has played down reports linking the Barcelona star with a move to Aston Villa.
The former Chelsea and Bolton forward looks set to leave Barcelona this summer after dropping down the pecking order at Camp Nou.
A host of clubs are thought to be keen on luring the 29-year-old back to the Premier League with Villa, Manchester City, West Ham and Portsmouth all credited with an interest in the player.
The player's father and agent, Arnor Gudjohnsen, says the Iceland international would be interested in a move back to England, but is unlikely to be keen on a switch to Villa for personal reasons.
"I have seen some speculation about Eidur and Aston Villa, but I don't think he was very keen on that," Gudjohnsen Snr told skysports.com.
"I think if he was to return to England he would prefer to go to London or Manchester rather than Birmingham.
"There are a few clubs interested, but there is nothing serious at the moment.
"I think he will leave Barcelona and he is keeping his options open at present."


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Domino Resources - Part 2
Some times you can not see all the text in a table because of the table width. Users can see the entire text whenever they click on that text. Remember this will help for computed fields. Use the following steps to show the text in a pop-up message box.
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Download: IT certifications that stand out
Are you looking to advance your career? Expand your knowledge? Boost your credibility? Our editors put together this complimentary 19-page guide on everything you need to know about obtaining an IT certification—with special attention given to cloud and desktop certifications.
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text: Which Caribbean island is your perfect match? Brought to you by Find a holiday in the Caribbean that ticks all the right boxes. You'll be able to discover how each island has its own unique personality and charm to find an island that suits you best. Find out about some of the most luxurious accommodation in the Caribbean and pick up holiday tips and inspiration How to choose your dream island Relaxing retreats 05 May 2017, 4:00pm Five all-inclusive beachfront hotels in the Caribbean 05 May 2017, 3:45pm Enjoy modern luxury at the oldest hotel in the Caribbean 05 May 2017, 3:15pm Revitalising escapes at Saint Lucia's most relaxing hotels Luxurious surroundings 05 May 2017, 3:10pm Discover the world's biggest open-air aquarium at this Bahamas hotel 05 May 2017, 3:00pm Escape to an adults-only hotel on the Mexican Riviera Maya 05 May 2017, 2:30pm Stay at the most romantic couples' retreat in Jamaica 05 May 2017, 2:15pm The ultimate all-inclusive luxury Caribbean holiday


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Wendy Makkena (born Wendy Rosenberg Makkena on October 4, 1958 in New York City, New York) is an actress who has guest-starred on Law & Order: Special Victims Unit and Law & Order.
She has starred on television shows such as Judging Amy, Oliver Beene, The Mob Doctor and NCIS.
Law & Order
- A Death in the Family (1991) as Officer Nicki Sandoval
- Born Again (2002) as Janet Weston
Law & Order: SVU
- Transitions (2009) as Ellen Van Kuren


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Mrs Buhari at the Women in Export Forum and Exhibition, organized by the Nigerian Export Promotion Council in collaboration with the International Trade Centre, made this comment however, warning that they should at the same time be mindful of their homes.
At the event, Nigeria was formally integrated into the International Trade Centre, a trade initiative, which aims to get one million women globally involved in international business by 2020.
The initiative is geared towards tackling the challenges faced by women in export, including funding, capital and land acquisition, cultural beliefs, gender imbalance and lack of support from men.
Nigeria is targeting the involvement of 100, 000 women in the initiative and most of the participants are hoping that the right support will come for the target to be attained.
Aisha Buhari Solicits Support For Female Entrepreneurs Reviewed by Link Naija on July 24, 2016 Rating:


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Skip to Content
Home > Services > Hospice > Hearts for Hospice
5 pm – Hors d'oeuvres
6:30 pm – Dinner
Steak De Burgo served with Roasted Red Potatoes
Chicken Florentine served with Orzo & Shiitake Mushrooms
Vegetarian Lasagna
Each dinner will include Balsamic Grilled Summer Vegetables, Broccoli Crunch Salad and Dessert.
Silent Auction
Quotes from the Comfort House
Live Auction
Two exciting trips will be offered during the live and silent auction. Are you looking for the perfect family vacation? How about a trip to Kennedy Space Center with hotel, airfare, lunch with an astronaut included? Or how about a long romantic weekend at Lake Tahoe?
View the Kennedy Space Center Trip here
View the Lake Tahoe Trip here
To view more items, go to www.bidpal.net/h4h2017
We appreciate your support of Hospice of Pella and look forward to seeing you on February 18!
Email our Foundation
© 2017 Pella Regional Health Center. All Rights Reserved.


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Cavendish — Cav en*dish, n. Leaf tobacco softened, sweetened, and pressed into plugs or cakes. [1913 Webster] {Cut cavendish}, the plugs cut into long shreds for smoking. [1913 Webster] … The Collaborative International Dictionary of English
Cavendish tobacco — Cavendish is more a process of curing and a method of cutting tobacco than a type of it. The processing and the cut are used to bring out the natural sweet taste in the tobacco. Cavendish can be produced out of any tobacco type but is usually one … Wikipedia
William Cavendish, 1st Duke of Newcastle-upon-Tyne — Infobox Nobility name =William Cavendish title = Duke of Newcastle upon Tyne KG KB PC spouse =Elizabeth Bassett Margaret Cavendish issue =Jane Cavendish Charles Cavendish Elizabeth Cavendish Henry Cavendish, Duke of Newcastle Frances Cavendish… … Wikipedia
Andrew Cavendish, 11th Duke of Devonshire — Andrew Robert Buxton Cavendish, 11th Duke of Devonshire, KG, MC, PC (2 January 1920 ndash; 3 May 2004), known as Lord Andrew Cavendish until 1944 and as Marquess of Hartington from 1944 to 1950, was a British Conservative politician. He was a… … Wikipedia
Frederick Cavendish Ponsonby — Major General Sir Frederick Cavendish Ponsonby, GCMG, KCB, KCH (July 6 1783 – January 11 1837) was a British military officer, the second son of the 3rd Earl of Bessborough and Henrietta Spencer.Early careerEducated at Harrow, he joined the army… … Wikipedia
Whist — Edmond Hoyle (1672 1769) Whist ist ein Kartenspiel für vier Personen, aus dem das Bridge Spiel hervorgegangen ist. Inhaltsverzeichnis 1 Geschichtliches … Deutsch Wikipedia
Трубочный табак — Содержание 1 Виды и сорта табака … Википедия
United Kingdom — a kingdom in NW Europe, consisting of Great Britain and Northern Ireland: formerly comprising Great Britain and Ireland 1801 1922. 58,610,182; 94,242 sq. mi. (244,100 sq. km). Cap.: London. Abbr.: U.K. Official name, United Kingdom of Great… … Universalium
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History of electromagnetism — The history of electromagnetism, that is the human understanding and recorded use of electromagnetic forces, dates back over two thousand years ago, see Timeline of electromagnetism. The ancients must have been acquainted with the effects of… … Wikipedia


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THE definitive, authoritative book on DSP -- ideal for those with an introductory-level knowledge of signals and systems. Written by prominent, DSP pioneers, it provides thorough treatment of the fundamental theorems and properties of discrete-time linear systems, filtering, sampling, and discrete-time Fourier Analysis. By focusing on the general and universal concepts in discrete-time signal processing, it remains vital and relevant to the new challenges arising in the field -- "without" limiting itself to specific technologies with relatively short life spans.
Table of Contents
1. Introduction
2. Discrete Time-Signals and Systems
3. The z-Transform
4. Sampling of Continuous-Time Signals
5. Transform Analysis of Linear Time-Invariant Systems
6. Structures for Discrete-Time Systems
7. Filter Design Techniques
8. The Discrete Fourier Transform
9. Computation of the Discrete Fourier Transform
10. Fourier Analysis of Signals Using the Discrete Fourier Transform
11. Discrete Hilbert Transforms
You can download this book from any of the following links. If any link is dead please feel free to leave a comment.
Download Here
Solution Manual: Download Here
Copyright Disclaimer
This site does not store any files on its server. We only index and link to content provided by other sites. Please contact the content providers to delete copyright contents if any and email us, we'll remove relevant links or contents immediately.


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EasyTerra Car Rental Greenfield-Park, QC is an independent car rental comparison site. Our system compares prices from well-known car rental companies so as a customer you can always reserve your rental car through us at a competitive rate.
EasyTerra Car Rental Greenfield-Park, QC compares prices of car rental companies at the following locations:
If you are looking for a rental car in Canada, but in a city other than Greenfield-Park, QC, please click through to the Car rental Canada page, where you can choose in which city in Canada you want to rent a car.


text:
Power tool in Cebu
Found:1 products← Back to category"Tools"
Glassdoor repair alingment and lockset installion
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By: Sophia B.
What is the size of a computer mouse, has a long, furry tail and is related to a chinchilla? A degu!
Degus originate from South America. Degus can be kept as pets and can also be found in zoos. They are illegal in Georgia, Alaska, Newfoundland, Labrador and New Zealand. No matter where they live they dig burrows.
Now you know where they live, but what do they look like?
Degus are small animals that are about the size of a computer mouse. They have small but sharp claws and have long, thin, and furry tails. If you pull on their tail, it will fall off and they will never grow it back. They have small eyes and long whiskers.
Baby degus are called "pups". The female degu is pregnant for about ninety days. When degus are born they are in an average litter of six pups. In the wild, degus can live up to thirteen years. While in captivity, they can live from six to eight years.
Like I said before, you can keep a degu as a pet. But first, you need to know how to take care of them. When you go to buy him/her, you also need to get them a cage, food, a food bowl and a water bottle. Their water containers usually look like baby bottles. In order to keep them healthy you should clean their cages once every week. Also, you should change their water and add more food to their bowl every single day.
When the little fur-ball(s) are being good you could give them a treat! Peanuts make excellent treats while something with sugar could make them even fatter. Plus, sugar is not good for degus.
Degu- a small rodent from central Chile
Originate- to bring into being
Burrows- a hole or tunnel dug in the ground by an animal
Captivity- the condition of being held captive


text: Is the photographs the inspire takes good enough resolution to print.? What resolution does the inspire take? Been concentrating on the video aspect of the inspire and a client is asking "resolution specs"of the Inspire. Any photo gurus got any gen? Are screen grabs good enough for print? Much appreciated. Thanks


text:
I think terms "...good-looking & handsome" are subjective to each individual's preference and somewhat vague.
My honest truth::
I'm looking for FWB situation, just plainly casual NSA fun, kinky date, or sugar-baby. I'd be fine with this if somehow it was turning into LTR thing.
I try to enjoy my life as much as possible and whenever I could.
Thinking of and exploring new things/places.
Besides that,
- Travel
- New ideas
you're looking for FWB situation.
you're looking for sex on the first date.
you're looking for someone to cuddle with.
you're looking for someone to go to swinging club with you.


text:
Carbon Block VOC Filter for use with RO-Pure Plus systems.
Premium high capacity carbon block formulated specifically for the reduction of VOC'S including Herbicides, Pesticides, TTHM, DPB and more.
Why use a VOC Filter? It is estimated that VOC's are present in one-fifth of the nation's water supplies. These water contaminants can enter ground water from a variety of sources including localized use of herbicides and pesticides, gasoline or oil spills, leaking underground fuel tanks, septic system cleaners, and chemicals used in the dry-cleaning industry.
RO-PURE Plus
Note: this filter also fits the RO-Pure System.
Conforms to NSF / ANSI 53 for VOC Reduction.
Filter Rating 600 gallons - 1 year
Easy to install
Flow rate .05 GPM (1.89 lpm)
The recommended filter change is once a year*.
Product Dimensions:
9 7/8 in x 2 in
Note: Do not use with water that is microbiologically unsafe or of unknown quality without adequate disinfection before or after the system.
*Note: Filter life may vary based upon local water conditions or use patterns.
This product will NOT fit the Watts 1/4 Turn KWIK Change Filter System.


text:
I like this one girl I got jealous she was hanging with other dudes and I told her that and we stopped texting. I want to text her but I don't know what to say
Most Helpful Girl
If you both aren't together (in a relationship) why are you getting so mad about her hanging with other men.. she can do as she pleases.
Jealousy can be cute but when it's over something such as that; that's a nono at least for me and a bit of a turn-off, cut her some slack.
I'd apologize and explain why you had gotten mad - I realize you like her but you can't control her.00|00|0


text:
Massachusetts governor signs anti-shackling bill
BOSTON — Gov. Deval Patrick has signed a bill outlawing the practice of handcuffing women prisoners to hospital beds during childbirth in Massachusetts.
The bill would create a uniform ban on restraining women during pregnancy, labor and delivery in state and county correctional facilities unless they present a specific safety or flight risk.
The bill also creates basic standards of prenatal and postpartum care to ensure safe, healthy outcomes for female prisoners and their newborns.
|The article you requested is no longer available on BostonHerald.com.


text:
Horns Amon AmarthT-Shirt
from 17,99 € You save 10% / 2,00 €
Product Details
|Outer material
|100% cotton
|Band merch, Vikings, Bands
|Melodic Death Metal
|Available since
|Product code
- with front and back print
- round neck
- short-sleeved
Amon Amarth play melodic Death Metal. Their lyrics are about Vikings. Exclusive to EMP you get this black Amon Amarth shirt "Horns". On the front of the t-shirt flaunts the typical Amon Amarth logo with two crossed horns. On the back you can see the Hammer of Thor surrounded with Viking characters. Especially cool is the fact that this shirt is available up to size 5XL.
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Written on:
I received this as a birthday present, and I'm satisified. I can tell it's a really thick, good quality material that's been used to make this t-shirt. It is a bit too tight, so I could do with the next size up, or maybe I just need to lose some weight!
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Written on:
Another great t-shirt from Amon Amarth and exclusive to EMP too. The front design has the band's iconic logo, a Viking helmet, stylized eagle's wings and crossed drinking horns against a background of Nordic beasts entwining. On the back is Mjolnir (no introduction required!) surrounded by a circular runic inscription which in fact says "Amon Amarth" four times. All in all it's an excellent design for any Viking -be the first on your longship with this t-shirt!
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How to Find Cheap Maternity Wedding Dresses
Many pregnant brides have a notion that it is going to be a difficult task selecting a cheap maternity wedding dress. There is plenty of choice available while choosing maternity wedding dresses today. There are many styles and fabrics available that can flatter your blossoming figure.
If you are pregnant and are worried that you won't find the 'Ohhhh, so gorgeous' dress, you are actually quite wrong.
There are many to-be-mom's walking the aisle as brides. Thus, designers have come up with really elegant and breathtaking wedding gown designs for women who are pregnant. And yes, they are not that expensive and won't burn your pockets.
Elegant Wedding Dresses
The pregnant bridesmaids also have many options available to look fabulous on their friend's wedding day. Many people think a pregnant friend and her baby will bring luck for their future married life. Thus, it is important to look your best at your friend's wedding. There are many affordable maternity bridesmaid dresses available which are simple, yet look elegant. These dresses are stylish, sensual and sexy. They are comfortable to wear and trendy in look. A-line dresses, princess lines and empire lines are the most preferred dresses that enhance the blossoming


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Residence Inn by Marriott Bethesda Downtown
(301) 718-0200
(301) 718-0200
Property Location This aparthotel is within the vicinity of Rock Creek Park and Montgomery College. Residence Inn by Marriott Bethesda Downtown is centrally located in Bethesda, walking distance from Consumer Product Safety Commission and close to American University.
Rooms Make yourself at home in one of the 188 air-conditioned rooms featuring kitchens with refrigerators and stovetops. 25-inch flat-screen televisions with cable programming provide entertainment, while complimentary wireless Internet access keeps you connected. Conveniences include phones, as well as desks and complimentary weekday newspapers.
Amenities Be sure to enjoy recreational amenities including an outdoor pool and a 24-hour fitness center. Additional amenities at this aparthotel include complimentary wireless Internet access, a picnic area, and barbecue grills.
Dining Grab a bite from a snack bar/deli serving guests of Residence Inn by Marriott Bethesda Downtown. Meet other guests and eat at a complimentary reception.
Business, Other Amenities Featured amenities include a 24-hour business center, express check-in, and express check-out. Planning an event in Bethesda? This aparthotel has 4099 square feet (381 square meters) of space consisting of conference space and meeting rooms.
Regular Hours
Fax: (301) 718-0679
Wi-Fi: Free
Specialties: extended stay hotel, hotel room, weddings, sports travel, family reunions, event and meeting services, overnight accommodations
Price Range : Average
Property Amenities: Express check-in, Picnic area, Express check-out, Elevator/lift, Free breakfast, Barbecue grill(s), Terrace, Laundry facilities, 24-hour business center, 24-hour front desk, Meeting rooms, Use of nearby fitness center (discount), Snack bar/deli, 24-hour fitness facilities, Conference space, Luggage storage, Dry cleaning/laundry service
Swimming Pool: Seasonal, Outdoor
Parking: Valet
No coupons available


text:
RBA's New Boss Greeted by Trader Bets That Cuts Almost DoneBy and
Swaps show 74% chance RBA rate won't go any lower in 2016
Yield on benchmark 10-year bond has climbed as high as 2.17%
The bond market is signaling that Reserve Bank of Australia Governor Philip Lowe, who only assumed control this week, is likely to preside over the end of his country's longest monetary easing cycle.
Swaps traders boosted bets the RBA is on hold for the rest of this year to 74 percent as of 3:30 p.m. on Wednesday in Sydney, up from 51 percent at the end of August. Australia's cash-rate target, which the central bank trimmed to a record 1.5 percent last month, is seen just 16 basis points lower in 12 months' time, close to the smallest easing bias seen this year.
Australia's economy is benefiting after commodity exports surged 30 percent from a trough this year and from unprecedentedly low interest rates, the RBA said Tuesday in minutes of the Sept. 6 policy meeting at which it left borrowing costs unchanged. The central bank is seeking to maintain a 25-year economic expansion that has faltered in recent years amid the collapse of a once-in-a-century mining boom and the spread of global disinflation.
"Given where rates are, the case to cut further has to be pretty compelling and when the data is mixed on the activity front it is clearly not a compelling case," said Su-Lin Ong, the head of Australian economic and fixed-income strategy at Royal Bank of Canada in Sydney.
Former central bank governor Glenn Stevens, who went into retirement at the end of last week, cut interest rates by 3.25 percentage points over the past five years. Lowe -- his erstwhile deputy -- indicated earlier this year that lowering the benchmark cash rate on its own would lose effectiveness as it approaches 1 percent. With the nation currently enjoying an unexpected boost from rebounding global commodity prices, the jobless rate falling and economic growth accelerating, some have speculated that August's quarter-point easing may have been the RBA's last for this cycle.
RBA Assistant Governor Christopher Kent said in an address last week that Australia is about three-quarters of the way through the unwinding of its mining investment boom and that the level of export prices relative to import prices is predicted to remain near current levels. Kent's speech was a signal that the central bank has now shifted to a neutral policy stance, according to Tim Toohey, Goldman Sachs Group Inc.'s chief economist for Australia.
"An adjustment lower in two keys factors -- capex and commodities terms of trade -- were the main reasons for RBA's easing cycle since 2011," said RBC's Ong. "While the two aren't tailwinds, we would argue the headwinds and the drag on activity from them are abating and that's quite significant."
The dialing back of easing expectations pushed the three-year sovereign bond yield to as much as 1.70 percent on Wednesday, the highest in almost three months. Its premium over the cash rate is near the widest since 2014. The 10-year yield this week climbed to a high of 2.17 percent.
Lowe will make his first public comments as governor on Thursday, when he's scheduled to appear before a parliamentary committee in Sydney.
"For those who like to parse the RBA's commentary, the positive flow outweighs the negative by a comfortable margin," Michael Blythe, Commonwealth Bank of Australia's chief economist, wrote in a note to clients Tuesday. "There is a growing perception that the need for additional interest rate stimulus is declining and the RBA's reluctance to provide additional stimulus is increasing."
— With assistance by Michael Heath


text:
10:15 p.m.
I finally got around to reading the Island Resident Orientation Guide. And now I totally understand why Harper was so interested in what I thought about it; she knew I would have a lot of questions after reading it! The first part of it was just an introduction to the village and community, the facilities available to us -- stuff that I'd already learned from living here for over three weeks. Then it started going into our purpose here on the Island and the things that make the Island "special", and that's where it started to get weird. Basically it's saying that our primary purpose is to protect the Island at all costs... peacefully when possible, but forcefully when necessary. We've been chosen to dedicate our lives to be a part of this special task, and anybody else who's not one of us cannot be trusted with knowledge of the Island in the outside world. It even explicitly says that any outside visitors who are not chosen to join us must not be allowed to leave the Island, so as to prevent knowledge of the Island from being leaked to the rest of the world. So, if that's the case, then how is it that those of us from the plane crash were given a choice of whether to stay or leave? Or did we ever even have a choice to begin with? Considering that all of us supposedly chose to stay, I'm not sure if that's something we'll ever really know!
Then there's this whole section about someone named Jacob, supposedly the man in charge of the Island, whom this community's leader, Benjamin Linus, reports to. It says that Jacob is the one who's entrusted us to protect the Island, and he lives in seclusion in a secret location on the Island, and only the community's leader reports to him. While this whole organizational concept in itself does sound rather strange... as soon as I saw the name Jacob, I just had this feeling that I can't really explain, that whoever this mysterious Jacob may be, his charge to protect the Island is somehow exactly what I'm supposed to do. I'm not sure what it is about the name Jacob that triggers this weird sense of trust; I've never actually known anyone named Jacob, as far as I remember. Wait, actually, that one American guy that I met at the hotel bar in Sydney... the one who actually convinced me to stay in Australia a bit longer... I think he said his name was Jacob... but who knows, I was drunk, and he was just a random stranger anyway. Anyway, it explains that Jacob is the one who chooses who gets to join this community of Island protectors, and obviously that means that I was on his list. It also mentions that children are automatically eligible to join us, and are, in fact, extremely important to the community, due to the current inability to procreate on the Island. I guess that would explain Diane's comment about the ten-year-old boy on the other side of the Island.
There's also a brief section about the fertility problem -- the one that causes pregnant women to die before they can give birth. The cause is still unknown and is being researched (and I already know that Juliet is the head of that research team, and Diane is a part of it), and standard birth control has proven to be ineffective, due to the increased male sperm count on the Island. Because of this, sex is highly discouraged, and we are encouraged to find "alternative ways" to control or satisfy any natural physical urges we may have. Great.
Anyway, that's as far as I've read so far. I'm kinda curious about the next two chapters, which are labeled "The Temple" and "The Black Smoke", but this was quite enough information to take in already. I'm definitely going to have plenty of questions for Harper next Monday!


text:
Absolute Rated Pltd Cart., 10 gpm, 0.2 Mic
PARKER| Zoro #: G2217731| Mfr #: PAB002-10FE-DO
Zoro #: G2217731Mfr. Part #: PAB002-10FE-DO
Technical Specifications
|Zoro #: G2217731 Mfr #: PAB002-10FE-DO
|Filter Media: Polypropylene
|Reduces: Particulates
|Inside Dia.: 1-1/16"
|Item: Filter Cartridge
|Filter Cartridges Sub-Category: Pleated Cartridges
|Max. Temp.: 160 Degrees F
|Max. Pressure: 35 psi
|Standards: FDA
|Microns: 0.2
|Outside Dia.: 2-11/16"
|Height: 10"
|Flow Rate: 10 gpm
|Country of Origin (subject to change): United States
Product Description
Absolute Rated Pleated Filter Cartridge, 0.2 Microns, Filter Media Polypropylene, Height 10 In., Outside Dia. 2-11/16 In., Flow Rate 10 gpm, Max. Temp. 160 Degrees F, Max. Pressure 35 psi, Reduces Particulates, Application Ideal for Membrane Prefilter and Chemical-Handling Applications, Inside Dia. 1-1/16 In., For Use With 10 In. Housings, Standards FDA
Restrictions and Compliance
- *1 : California Proposition 65 : This product contains a chemical known to the State of California to cause birth defects or other reproductive harm.
- *2 : California Proposition 65 : This product contains a chemical known to the State of California to cause cancer.


text:
School Backpacks & Lunch Kits 40% Off @ Bentley Canada
Even though the kids just got out of school, Bentley has already thought this one out. They are offering an excellent selection of backpacks and lunch kits on sale for 40% off so that you can beat the back to school rush in the early fall. You'll want to view the entire selection, they have quite a variety.
Your child will really like the look of this Tracker Lunch Tote that is on sale for $19.19. The reg. price is $31.99. This style of lunch kit is so much easier to pack food into, and clean, because of the wide opening. They can select to have an owl, or a pink poodle!
Is your child a cat lover? Then this Tracker Cat Themed Backpack is going to be a great match for them! It's on sale for $31.19, and the reg. price is $51.99.
Shipping is free at Bentley if your order is
over $75. Otherwise, shipping is a flat-rate shipping of only $10.
(Deal expiry: July 10th, 2016)
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text:
Flounder is a fish, who lives under
the sea, in Atlantica. He is a
funny little fish, with blue hair and stripes,
a roley poley backside, a roley poley
head, and a roley poley bottom. He is a
naughty little fish as well. He is always seen
pulling carts around, preparing for
the bigger sea creatures to go
on long adventures. And as
soon as carts arrive, he takes
them away, so the bigger sea creatures
can have a nap. Flounder thinks no one
is better than him. He likes to try
to fool them, especially Ray Ray,
the stingray. Flounder likes to sing at him. C'mon lazy
guy! Why don't you do some work? One day, when he had
finished pulling wagons, Ray Ray came back very
sleepy, when Flounder arrived in his usual, very naughty
way. C'mon lazy guy! Do something! Try to catch me!
And he swam off singing. Instead of going
back to sleep, Ray Ray thought of a way
he could pay Flounder back. One morning,
Flounder couldn't get up.