text:
Remodeling Services
Need a new kitchen and Bath? Well you came to the right place!!
A&D Kitchen Bath Remodeling can make your dream kitchen and bath come true. We also do floors, exterior and interior painting, roof, presure wash and more. For more information please call us!
Monday - Friday
8:00 A.M. to 7:00 P.M.
(Tuesday we close at 5:00)


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Advanced Search
The figures come via IDC's new quarterly cloud infrastructure tracker.
What are we likely to see when it comes to the "Internet of Things," cloud computing and careers?
Big Switch Networks Inc. capped off a big year in the software-defined networking (SDN) industry by announcing its flagship networking fabric was awarded certification for Red Hat Enterprise Linux OpenStack Platform 5, laying the groundwork for OpenStack cloud computing implementations.
2015 is no longer on the way -- it's here. What do some leading virtualization and cloud industry experts expect to see this year?
Dan sees more infiltration of virtualization technologies into the enterprise in the coming year.
Central to its predictions is the connected, virtualized datacenter.
Is "software-defined" just a marketing catchphrase, or does it have real promise?
Mitch Parker of Citrix believes the platform "will offer APIs to connect to all of the on-premises datacenter elements seamlessly."
Hardware giant Dell Inc. this week announced a partnership with network virtualization company Midokura that brings its MidoNet software-defined networking (SDN) technology to Dell systems as an overlay option for OpenStack environments.
A new product for automating the software delivery process is officially released as well.
Amazon Web Services (AWS) Inc. this week said its OpsWorks application management service will now be able to work in on-premises servers.
If you haven't looked at or used Microsoft Azure lately, you're missing out. See what it offers, and find out if it will benefit your business.
Dan says it's a clever play to lure developers to the open source cloud platform.
The cloud price wars continued apace this week, with market leader Amazon Web Services (AWS) Inc. slashing data transfer costs significantly.
It also builds functionality for Docker Machine, used for remote container orchestration, into several core offerings.
Amazon Web Services (AWS) Inc. this week introduced two new payment options for its EC2 Reserved Instances service.
CoreOS, in announcing the move, says that Docker has lost sight of its core mission of providing simple application containers.
CloudStack and Eucalyptus have lost significant market share in the last two years.
Although inconceivable to some, open source may be a weakness rather than a strength.
The company calls it "a unified development and operations experience."
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subset: commoncrawl


text:
Al Dente Map
3201 New Mexico Ave. NW (Nebraska Ave. NW)
202-244-2223 | Make Restaurant Reservations | Menu
Now that you know where you want to enjoy a meal, it's time to find the restaurant. Print out this Al Dente map and take the driving directions with you so you can locate the restaurant easily.
Find out which restaurants and chefs top the list of GAYOT's 2016 Annual Restaurant Awards.


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BETHESDA, Md.—As part of Host Hotels & Resorts' efforts to transform the Denver Marriott Tech Center Hotel, the Company has selected Denver-based Sage Hospitality to manage the hotel pursuant to a Marriott franchise agreement. Beginning in late 2015, the hotel ...Read More »
Category Archives: Industry NewsFeed Subscription
Dunavant Enterprises Selects Salamander to Manage Florida Resort
DESTIN, Fla.—Dunavant Enterprises has selected Salamander Hotels & Resorts to assist with the development of and manage the anchor hotel of its new multi-phase Henderson Beach Resort in Destin, Fla. The 171-room beachfront luxury property is scheduled to opened in ...Read More »
Joie de Vivre Announces Miami and New York City Locations
SAN FRANCISCO—Commune Hotels & Resorts is bringing its native California brand, Joie de Vivre Hotels, cross-country as part of a national expansion. The addition of two Joie de Vivre hotels in primary gateway cities will establish the brand's foothold on ...Read More »
Former Viceroy CEO Launches Proper Hotels Brand
LOS ANGELES—The newly formed Proper Hospitality has announced the launch of Proper Hotels. Led by CEO Brad Korzen, the creator and former CEO of the Viceroy Hotel Group, along with his partners Brian De Lowe and Alex Samek, Proper Hospitality ...Read More »
RLHC Takes Steps to Accelerate Growth Strategy
SPOKANE, Wash.—Officials at RLHC (Red Lion Hotels Corporation) have announced that the company has completed a comprehensive transaction to accelerate the execution of its national growth strategy. Key components include selling a 45 percent ownership stake in 12 hotels wholly ...Read More »
Country Inns & Suites By Carlson Signs Nine Hotels
MINNEAPOLIS—Country Inns & Suites By Carlson, the upper-midscale hotel brand from Carlson Rezidor Hotel Group, has announced the signing of nine hotels across North America. "Expanding our footprint in the U.S. is a key priority for the brand this year," ...Read More »


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Coronation Street
The Nazir household may have been dealing with the repercussions of Sharif's affair, but Alya still looked great in a sparkly black sequin top with lovely beaded fringe detail.
We know so many people will be interested in this (it's gorgeous!) and we can tell you that this was available earlier in 2016
Also featured: 17 Feb 2017


text:
Direct Search
You need to login to order products. Go to the signup page to sign up today!
Tantus - Bend Over Beginner Harness Kit Purple Haz
Tantus - Bend Over Beginner Harness Kit Purple Haz
The Bend Over Beginner Kit was designed for those who would like a 'starter' kit, so you can pace your exploration with comfort and safety. The kit includes an adjustable, machine-washable harness, a Silk Small and Silk Medium, and a bullet vibrator that fits into a small pocket in the harness. There's also an extra o-ring, so that the harness can be used with toys of different diameter.
The harness fits up to 152,4 cm hips.
Silk Small: 10,8 cm insertable length, 2 cm diameter
Silk Medium: 14 cm insertable length, 2,5 cm diameter
Bullet with battery included.


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DS-13 -13- Demon System 13 MP3
from "Aborted Teen Generation"
Demon system 13 - I hate you MP3
Demon system 13 - I hate you. Vad Vet Vi Om Kriget?
DS-13 reunion feat. Dennis Lyxzén from Refused MP3
D.S.-13, aka Demon System 13, doing the following songs at their reunion show at Verket, Umeå, Sweden, April 5, 2014: 1. "Young 'til I die" (7 Seconds) 2.
Straight and Drunks MP3 - Demon System 13 MP3
Demon System 13 - Work Buy Consume And Die MP3
Crappy video I did with something from the site adbusters, pictures of booklet of their CD and internet photos... And a picture of my notebook that I used while ...
Demon System 13 - degenerated generation MP3
This is Umea, Sweden's DS-13 Degenerated Generation video. This was a class project I did a few years back using After Effects for motion graphics class, I sent ...
Demon System 13 - Suiciety made us do it MP3
Demon System 13 - Suiciety made us do it. Vad Vet Vi Om Kriget? LP.
Demon System 13 - Straight and Drunks MP3
Thrash and Burn EP.
Demon System 13 - Work Sucks! MP3
Thrash and Burn EP.
DS-13 - I Was A Teenage Fistfukk CS MP3
A.K.A. "Demon System 13". DS-13 is a hardcore punk band from Sweden that later became a fantastic thrashcore band; however, their earlier stuff is a fucking ...
2012 System Demons 13 - Possession Demon MP3
Due to Mind Possession in the comments section - comments have been disabled. For discussions please join us on the Demonology Forum System Demon 13 ...
Geometry Dash Demon [Medium] - Chaotic System - by GironDavid (All Coins) MP3
Please Gron, make ur levels harder.. This was too easy ;3; Total Attempts: 752 ☆---------------------------------------☆ ▻Name: Chaotic System ▻ID: 13308332 ...
'Demon-Off!' Self Deliverance System MP3
Are you drug-addicted, struggle with anger, depression, suicidal thoughts, or have physical or mental infirmities that doctors don't know how to treat? You may ...
Astronaut 13 100% GAMEPLAY Online (Minesap) INSANE DEMON MP3
OMGGGG!!! I am the first (1st) the complete it!! The wave parts are INSANE!!! The wave-ship part is creative and easy! Anyway good level. MINEPAI NOTICE ME!
Demon's Souls Drunkthrough - Part 13: Tower of Latria MP3
The following Drunkthrough is performed by a trained professional, do not try this at home. Always drink responsibly. Catch Drunkthroughs LIVE every Friday ...
Call of Duty: Black Ops 3 - Walkthrough Part 13 - Mission 8: Demon Within Part 2 (60 FPS) MP3
Call of Duty: Black Ops 3 - Walkthrough Part 13 - Mission 8: Demon Within Part 2 (60 FPS) Walkthrough of Call of Duty: Black Ops 3 in High Definition on the ...
Sector 13 100% GAMEPLAY Online (JoarZ)*REAL WAY* MEDIUM DEMON MP3
Guess what! This level is not demon but it should be! JoarZ is an awesome Creator and thats why I decided to beat it. NOTICE JOARZ REBTRELL.
Demon's Souls Live Playthrough - 13: Storm King and Satsuki MP3
This is my live playthrough of Demon's Souls on the playstation 3. It is the precursor to Dark Souls and should be owned by anyone with the system. If this let's ...
BEATING THEORY OF EVERYTHING 2 - Geometry Dash #13 MP3
Another demon completed! Now all that's left is Deadlocked. I'm sure THAT will be tons of fun... (Sorry if this video is crappy.) Game Link: ...
[13] Lo Wang, Demon Decimator (Shadow Warrior w/ GaLm) MP3
"What is Shadow Warrior?" -------------------------------------------------------------------------...
SECRET WAY DEMON - SECTOR 13 - by JOARZ MP3
Secret way demons are so gud. Sorry for not including the ID but it's at the end where I comment on the level... Now go get dem free stars l3l.
Diablo 3 Beta PATCH 13 - UI, Runestones, and Demon Hunter skills Part 2 MP3
Here I get my first runestone and explore how the system works in detail. More Patch 13 videos coming soon, the Demon Hunter is fully recorded through to the ...
Matt Demon - Heaven or Hell MP3
vom Album "Fuck The System" http://bigredmachine.eu/product_info.php?info=p67_cd-matt-demon---fuck-the-system.html -uploaded in HD at http://www.
Diablo 3 Beta PATCH 13 - UI, Runestones, and Demon Hunter skills Part 1 MP3
In this video we go over the UI changes and how the new skill system works. I also go over how to use Elective Mode to change your skills, but since I didn't ...
Let's Play Demon's Souls Part 13 - Fool's Idol Boss Fight, Baby's Nail Phantom, Tower of Latria 3-1 MP3
Watch more Demon's Souls! https://www.youtube.com/playlist?list=PL5dr1EHvfwpMuVmxZzzqfMVUR48EaIbJc Like, Comment and Subscribe for more content!
[Geometry Dash [2.0] Astronaut 13 (Medium Demon) by Minesap | MinebraineGM MP3
Mi mayor fail en este Demon fue al 65% 2 veces Ez Pz ;) ○ ID: 6988264 ○ RATE: DEMON ☆10 Gracias por ver este video, recuerda dejar tu like, suscripción y ...
Tracy Twyman | Communing With The Demon Masters Of The Elite & The Portal To Hell MP3
Well known conspiratorial author and esoteric historian, Tracy Twyman, joins THC to talk about her 13 year personal journey to communicate with the same ...
Demon's Souls Beginners Guide Part 13: How to Defeat Armor Spider MP3
My walkthrough of an amazing game, actually, one of my favorite games of all time, Demon's Souls, this is part 13: How to Defeat Armor Spider, enjoy...... Current ...


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Flash Furniture FD-CH0221-4-GV-005-GG HERCULES Series 21" Extra Wide Plum Stacking Church Chair - Gold Vein Frame
Ships in 2-3 days
Availability: In Stock
✔ Gold vein finished 16 gauge steel frame✔ Plum fabric upholstery✔ 5" thick waterfall seat✔ Book pouch on backSee more product details
Earn $0.39 Loyalty Reward Points! More Info
Customers who bought this also bought:
Item Details
A versatile seating option, the Flash Furniture HERCULES Series 21" Extra Wide Plum Stacking Church Chair is perfect for banquet rooms or conference settings. Boasting plum fabric upholstery, this chair highlights any space and its book pouch on the back provides extra space for storing personal items.
Thanks to its thick padded waterfall seat, this piece relieves pressure on your legs for a comfortable sitting experience. For long-lasting performance, this stacking church chair is built with a gold vein 16 gauge steel frame for exceptional strength and has plastic rocker glides that are easy on your floor.
- 800 lb. Church Chair
- Gold vein finished 16 gauge steel frame
- Plum fabric upholstery
- 5" thick waterfall seat
- Book pouch on back
- Plastic rocker glides
- Ganging clamps attaches several chairs together
- CA117 Fire Retardant Foam
- Dimensions: 21" x 33" x 24-3/4"
- This item can be personalized
- Brand:
- Flash Furniture
- Model #:
- Ship weight:
- 19 lbs 13 oz
- Sold As:
- 1 ea
- Item Number:
- Weight Capacity:
- 800
- Size of Seat:
- 21"W x 16.50"D
- Size of Back:
- 21.25"W x 17"H
- Height of Seat:
- Depth:
- 25"
- Width:
- 21.25"
- TigerChef ID:
- 135715


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It's not every day that you realize that you have to buy a replacement Relay to fix your automobile. When your Plymouth PB100 was born, it was destined for a person with discerning taste who prioritizes economical pricing in their automobile. Plymouth automobile owners have become used to a certain level of reliability when driving their PB100 around town. While it's not hard to buy replacement parts for Plymouth vehicles, these days the necessity for nothing but the absolute best are required to repair your car or truck.
Buy all the car parts you will ever need from carpartsdiscount.com and save time and money on all your upcoming repairs. When your Plymouth requires the best replacement Relay, there's no need to go somewhere besides the authority, Car Parts Discount. Don't let a Relay repair spell the end of your car, truck, or SUV, and don't gamble with its condition by using anything but the best replacement parts. When it comes to Plymouth PB100 Relay parts, you can find them for 1980, 1979, 1978, 1977, 1976, 1975 models by clicking the appropriate year below.


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The Irish Beer Pub set features a classic shape and are an essential in any well equipped bar. The shape of these glasses allow for optimal aroma release, making them perfect for enjoying your favourite dark beers. Crafted of glass, this set is elegant enough for entertaining guests yet sturdy enough for your everyday, beer drinking needs!
- Brand: Trudeau
- Multicolour
- Glass
- Set of 4
- 16 oz capacity
- 6" height
- Dishwasher safe
Reviews (1)
Great set for any beer lover!Posted on October 18, 2016 by Crystal


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Report summaryTotal primary energy demand in 2014 declined, predominantly due to unusually mild temperatures. Demand will recover in 2015/2016, based on a return to normal temperatures, although mild temperatures in December 2015 will limit the extent of the recovery. However, in the longer term UK energy demand reflects a mature and developed economy and we expect a downward trend.
The UK's economic structure continues to fundamentally shift, away from heavy industry to services which has been accompanied by a fall in energy intensity. This economic transition also shifts the balance of energy demand from industry to the RCA sector, where rising population and a growing number of households will result in wider use of white goods but improving device efficiency will offset some incremental demand.
What's included?
This report includes 3 file(s)
- United Kingdom energy markets long-term outlook H2 2015 PDF - 275.62 KB 3 Pages, 1 Tables, 0 Figures
- United Kingdom energy markets long-term outlook H1 2015.pdf PDF - 485.29 KB
- United Kingdom Energy H2 2015.xls XLS - 833.50 KB


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|This article contains Indic text. Without proper rendering support, you may see question marks or boxes, misplaced vowels or missing conjuncts instead of Indic text.
ਸਿਰਜਨਹਾਰ: "Srijan-har"
Srijan - To establish, to give birth
Har - This is used as a suffix meaning the one who can do whatever the prefix refers to. So, in this case, It refer to "the person or being" who can "establish" or create or refers to the "Creator"
See Also
|These are the Popular Banis of Sikhism
Mool Mantar | Japji | Jaap | Anand | Rehras | Benti Chaupai | Tav-Prasad Savaiye | Kirtan Sohila | Shabad Hazaray | Sukhmani | Asa di Var | Ardas


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The only power we have is over our mind. We do not have control over outside things. Sadly, we have surrendered this only power and are slaves of the mind. Control the mind and we will find our strength.
Mind plays innumerable tricks on us. Learning to dodge these tricks and reaching the goal post is self-realization.
Before trying to know others we need to be aware of ourselves.
When we see and accept our faults, we take the path to bliss.
Instead of fighting with others, we should fight with our mind and get it disciplined.


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This week the government doubled its investment in bailing out the already part nationalised RBS and Lloyds Banking Group, injecting a further £37bn into the ailing banks. With the taxpayer footing the bill for one of the world's biggest government rescues, the issue of bonuses has reared its ugly head once more.
As part of the new deal, the government sought pledges from the banks that staff earning £39,000 p.a. or more would not receive cash bonuses, and executive directors would have bonuses deferred for three years. Despite the proposed 'crackdown', pay experts and politicians remained unconvinced. "This is all hot air. This has nothing to do with risk management and everything to do with populism", Liberal Democrat shadow chancellor Vi...
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THEY'RE set to inherit up to $US1 billion at 21 but the twin teen heirs of tobacco heiress Doris Duke say they were locked in a feces-strewn basement and scalded by boiling baths at their heroin-addicted dads sprawling plantation.
"I never asked to be born into any of this. Sometimes I wish I was never born," 15-year-old Georgia Inman tells Rolling Stone magazine in an interview that hits newsstands today.
Calling her fortune "blood money," Georgia said her crazed father, Walker Inman, Duke's nephew, was so horrific that she once put a gun to his head in an unsuccessful attempt to end the kids' misery, The New York Post reports.
"They. Stuck my brother and I. In hot boiling water in our bath," Georgia recalled in a faltering voice in the interview.
"It felt like our skin was melting away," she said, her body shaking from the memory of the abuse she and her brother, Walker Patterson, suffered at the hands of their dad and, allegedly, his fifth wife at the Duke family estate in South Carolina.
The troubled duo were sent to a mental hospital for three months of recovery from a childhood of trauma.
And they've yet to fully heal. Both teens have recently contemplated suicide and suffered from anorexia.
The cruel dad - who received an estimated $US90,000 monthly inheritance - got custody of the kids in 2000 when they were 2.
He had custody until he died from a meth overdose in 2010.
They now live with their money-hungry ex-stripper mum, Daisha Inman, Walker's third wife, in a $US20,000-a month Utah rental.
Inman is currently locked in a vicious legal battle with executives from Citibank and JPMorgan - who administer the twins' hefty trust funds - in Manhattan Surrogate's Court over her handling of their dough.
Titled "Poorest Rich Kids in the World," the explosive article includes horrified recollections from employees who worked at Walker's estates.
"Walker made them stay down in the basement all the time," wrote plantation caretaker Vick "Butch" Deer in an affidavit related to a onetime lawsuit over damage at the property.
"The basement was covered in feces, and it was smeared all over, and it smelled terrible. It was so bad that I wouldn't leave a dog in that condition."
Several others said the kids were locked in their room each night, and, according to a former nanny, "There was food strewn across the floor and a foul smell from where the kids had been relieving themselves in a corner."
The article opens with a harrowing tale of Walker's fifth wife, Daralee, driving the kids to school one morning - already allegedly wasted at 7:30am - and crashing into a tree.
The kids had a pet lion cub and brought diamonds to school for show-and-tell, but they looked malnourished with dark circles under their eyes to the stream of nannies who cared for them.
And they're still living a cloistered life. Georgia told the magazine that she'd never heard of the childhood game Musical Chairs, and Walker Patterson's fondest memory of Pop was when he intentionally set off a tear-gas grenade in the house as part of a whacked-out safety lesson.
Three years from adulthood, they both still believe in Santa Claus. "Dear Santa, I know I haven't been good, but if you do come all I want is to say hi to you in person," Patterson recently wrote to the mythical character, according to Rolling Stone, in the shaky handwriting of a first-grader.
Georgia claims she once spotted the bearded man in the flesh, but it's more likely the vision was an apparition of her late father, who's haunted her since his untimely passing.
"I think he's here," she whispered during the magazine interview, gesturing to an empty chair in her new Utah home.
The interview poses the obvious question: Why didn't the authorities intervene to rescue these poor little rich kids?
Wyoming law-enforcement never filed charges against the Inmans, who lavished local staff with exorbitant salaries and benefits.
When Walker brought his family to the South Carolina plantation, the Department of Social Services logged three reports about the crazy clan.
In one incident, police were called to a restaurant after Georgia's dad slapped her so hard, fellow diners feared for her life.
Daisha's plans for her kids' future? Pairing them up with fellow fatherless children with a similar amount of baggage.
"She's working on getting the twins together with Michael Jackson's kids, with whom she thinks they'd have tons in common," the article concludes.


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Round white pot with snow motif on the top
White pot with snow motif on the top
Product information:
Height: 15 cm
Diameter: 9 cm
Color: white
Care instructions:
Handwash only.
People & Planet:
The material in this product MAY BE recyclable. Please check the recycling rules in your community and if recycling facilities exist in your area.
Share this with your friend with social media


text:
Portuguese Garbanzo Bean Salad With Roasted Garlic
Recipe by dicentra
365 Ways to Cook Vegetarian, Kitty Morse
Top Review by Chef Swank
I love the whole idea, but mine didn't turn out quite right. It lacked "body" and wasn't tangy enough. I added lemon juice for flavor and a little chopped onion for crunch and think that helped a lot. But I still felt like it needed something to sort of bind it all together. Maybe I did something wrong! I suspect maybe I made a mistake with the roasting of the garlic. But I did enjoy it and would make it again.
See Discussion:1 Review
Ingredients Nutrition
- 1 head garlic (papery outside husks removed)
- 30 ounces garbanzo beans (drained)
- 1 tablespoon chopped fresh basil or 1 teaspoon dried basil
- 2 tablespoons balsamic vinegar
- 3 medium tomatoes (cut into 1/2-inch dice)
- 1 teaspoon ground cumin
- 1 teaspoon salt
- 1⁄2 teaspoon pepper
Directions See How It's Made
- Preheat oven to 425°F Slice 1/4 inch off top of head of garlic. Wrap garlic in foil. In a small baking dish, roast garlic until it turns buttery soft, 30 to 40 minutes. Let stand 10 minutes to cool.
- Meanwhile, in a serving bowl, mix garbanzo beans with basil and vinegar. Set aside.
- When garlic is cool enough to handle, squeeze out each clove into bean mixture. Stir well to coat beans.
- Toss with diced tomatoes. Season with cumin, salt, and pepper. Serve at room temperature.


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Alex Legends
Elevate Your Mind
Building The Community
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This is an important announcement to our valued listener and community member. We always appreciate your input and support in matters relating to Alex FM. Continue Reading
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The Maimane Alfred Phiri (MAP) Games
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text:
Norwich Vacation Packages
Norwich is located approximately 300 miles (483 kilometers) from Ottawa, the country's capital, and about 75 miles (121 kilometers) from Niagara Falls.
You don't have to limit your tour to the attractions of Norwich because the entire region has been graced with numerous captivating things to do and see. Two places within a relatively short distance of Norwich are Brantford, 20 miles (32 kilometers) to the southwest, and Thamesford, 20 miles (32 kilometers) to the east.
While you are in Norwich, don't forget to enjoy Kitchener. Approximately 30 miles (48 kilometers) to the north, this regional city is where you'll find TheMuseum, Conrad Centre for the Performing Arts and Centre In The Square. Should you have the time, be sure to visit Chicopee Ski and Summer Resort and Kitchener Memorial Auditorium Complex before returning to Norwich.
If the weather looks good, head outdoors and discover Southwestern Ontario's open-air museums and open-air exhibits.
In these hectic times, the perfect holiday often means finding great spots to relax. When it's time to kick back, copy the locals and try picnicking or bird watching. There are times when one of the most effective ways to understand a place is by tapping into the expertise of locals. This region has some fascinating winery tours, culinary tours and cycling tours.
For those who intend on arriving and departing by plane, it's good to remember that the handiest major airport is London International Airport, 30 miles (48 kilometers) to the west of Norwich.


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We can help you to find a recorder teacher in County Londonderry and across the UK. First Tutors: Music profiles County Londonderry recorder tutors and others offering popular instruments like piano, violin and guitar. Grade and diploma recorder lessons are all catered for across County Londonderry as well as recorder tuition just for fun. Use our site to search for recorder tutors near you and select which recorder teachers best suit your needs.
This is the best place to find the most talented recorder tutor for your needs in County Londonderry, whether you want recorder lessons to learn a new instrument, develop a hobby or train as a musician.
We list recorder tutors throughout your area by their cost and distance from you. Offered instruments include piano, violin and guitar for graded music exams or just for fun.
If you are a County Londonderry recorder tutor wishing to offer your recorder lessons in County Londonderry or anywhere else across the UK, please register with First Tutors: Music to commence giving recorder lessons or find out more about us here.
Towns: Coleraine, Limavady, Londonderry, Maghera, Magherafelt, Portstewart


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Sideshow Ollie
Have Ian Holloway's antics become a distraction to Blackpool? Steve Bates told the Supplement it's not far off.
Last Updated: 30/01/11 12:04pm
Ian Holloway is danger of turning from media darling to a major distraction at Blackpool.
That was the verdict of the Sunday People's Steve Bates at the end of a week in which the Seasiders boss threatened to resign, resigned, but had it turned down by the club.
Holloway and Blackpool have been fined £25,000 by the Premier League for fielding a weakened side against in November, despite the manager's very public protestations.
He remained true to his word on Friday but Blackpool chairman Karl Oyston refused to let his manager walk away but Bates believes Holloway had over-reacted - and as a result is in danger of damaging the goodwill towards him and his players.
"He's a character, but I think the danger is he might be becoming too much of a sideshow to what Blackpool are actually achieving," he told the Supplement.
"Listen, I'm not knocking him because he and his team have been great for the Premier League this season, but you have to have credibility in football.
"Everybody knew he wouldn't resign; why would he walk away from such a success story like Blackpool have been this season? Everybody knew that, I think he was just making his point.
"He is in a danger of becoming a kind of caricature of himself."
Holloway is also at the centre of one of the January transfer window's biggest sagas, surrounding club captain Charlie Adam.
The Scot has been the subject of a bid from Liverpool and although he has told them he wants to leave, an improved offer and a stand-down on the Seasiders' part would need to happen before he heads to Anfield.
Holloway, it has been revealed, is on a percentage of any money Blackpool make from selling their players - and that, says Bates, is another major concern at Bloomfield Road.
"I find it difficult to believe he won't go because you're denying a player like Adam - who he got for £500,000 - the chance to play on the big stage," he said.
"That would be a serious blow to Adam if he stopped him going; Adam is the heartbeat of the side, he is the club captain and you have to question what kind of response he'd get if he stopped him going.
"I understand why he doesn't want him to go obviously, but then linked into that is the fact that he gets a cut of the money.
"It's not illegal but it's sharp practice really and kind of goes against the grain of things. How many other managers do this? It has muddied the waters."


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Financing: $120,000.00 Down and
Reason for Selling:Owner retiring
Established deli and neighborhood grocery store with lottery and beer license. The store has full equipment set up for the preparation of hot and cold take out food items. Has loyal and regular customer base. Has good passing trade. Located in well populated residential area. Owner retiring. Business priced to sell quickly. Business has great potential for expansion.
Disclaimer: The information in these listings have been provided by the Seller and Vested Business Brokers, Ltd. has not independently verified this information and has no responsibility for its accuracy or completeness. Vested recommends that you consult with an accountant, attorney and other professionals to represent and assist you in any transaction. Vested's associate brokers will arrange a meeting between you, your representatives and the Seller, but is not authorized to make any representations or warranties. You agree to hold Vested and its associated brokers harmless of any misrepresentations made by seller.
1(877) 735-5224 | 50 Karl Ave Smithtown, NY 11787 | vestedbb.com


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I work with this girl so I can't exactly avoid her. So are there any tips anyone has to getting over a girl? Has anything worked from your personal experience?
Most Helpful Girl
Since you can't really avoid her, try and act as normal as possible. Put yourself out there and try to find a woman that will be interested in going out with you.00|01|1
Is this that sort of fake it till you make it concept. If you act like everything's normal you'll eventually start to believe it?
Most Helpful Guy
Just hang with the bros man. Rejection sucks. :(00|00|0


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Body in History : Europe from the Palaeolithic to the Future
Üye Girişi yapın, temin süresi ve fiyatını size bildirelim.
Üye Girişi yapın, sizi bu ürün stoklarımıza girdiğinde bilgilendirelim.
Temin süremiz 28 - 42 iş günü
Yayıncı Cambridge University Press ( 11 / 2015 ) ISBN 9780521124119 | 27,9 x 21,5 cm | İngilizce | Türler Arkeoloji
This book is a long-term history of how the human body has been understood in Europe from the Palaeolithic to the present day, focusing on specific moments of change. Developing a multi-scalar approach to the past, and drawing on the work of an interdisciplinary team of experts, the authors examine how the body has been treated in life, art and death for the last 40,000 years. Key case-study chapters examine Palaeolithic, Neolithic, Bronze Age, Classical, Medieval, Early Modern and Modern bodies. What emerges is not merely a history of different understandings of the body, but a history of the different human bodies that have existed. Furthermore, the book argues, these bodies are not merely the product of historical circumstance, but are themselves key elements in shaping the changes that have swept across Europe since the arrival of modern humans.
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Identification of genes specifically or preferentially expressed in maize silk reveals similarity and diversity in transcript abundance of different dry stigmas
In plants, pollination is a critical step in reproduction. During pollination, constant communication between male pollen and the female stigma is required for pollen adhesion, germination, and tube growth. The detailed mechanisms of stigma-mediated reproductive processes, however, remain largely unknown. Maize (
Transcript abundance profiles of mature silk, mature pollen, mature ovary, and seedling were investigated using RNA-seq. By comparing the transcriptomes of these tissues, we identified 1,427 genes specifically or preferentially expressed in maize silk. Bioinformatic analyses of these genes revealed many genes with known functions in plant reproduction as well as novel candidate genes that encode amino acid transporters, peptide and oligopeptide transporters, and cysteine-rich receptor-like kinases. In addition, comparison of gene sets specifically or preferentially expressed in stigmas of maize, rice (
Many of the novel genes uncovered in this study are potentially involved in stigma-mediated reproductive processes, including genes encoding amino acid transporters, peptide and oligopeptide transporters, and cysteine-rich receptor-like kinases. The data also suggest that dry stigmas share similar mechanisms at early stages of pollen-stigma interaction. Compared with Arabidopsis, maize and rice appear to have more conserved functional mechanisms. Genes involved in amino acid and lipid transport may be responsible for mechanisms in the reproductive process that are unique to maize silk.
In angiosperms, stigmas discriminate between compatible and incompatible pollen grains by preventing the adhesion, hydration, penetration, and growth of incompatible pollen
Because many genetic, cellular, and biotechnological tools, as well as a maize reference genome (inbred line B73), are currently available, maize has been proposed as a model grass to study the function of the dry stigma in reproduction
Several mechanisms related to pollination and fertilization has been implicated in pollen germination, pollen tube guidance, and pollen-ovule interactions
In this study, RNA-seq was performed to analyze transcript abundance profiles of mature silk (MS), mature pollen (MP), mature ovary (MO), and 6-day-old seedling (SL). To build a profile of candidate silk genes that are involved in early pollination events, genes expressed in maize silk were compared with those expressed in the other three tissues. We identified 1,427 genes that were specifically or preferentially expressed in maize silk. Bioinformatic analysis of the maize silk dataset not only identified pathways and genes that have been shown to be essential for pollination, but also a number of genes with novel or previously-unidentified functions, such as those encoding amino acid transporters, peptide and oligopeptide transporters, and cysteine-rich receptor-like kinases (CRKs). In addition, we characterized conserved and distinct features of dry stigmas by comparing stigma-specific/preferential datasets of Arabidopsis, rice, and maize.
Results and discussion
Transcriptome analysis of reproductive organs and seedlings in maize
To identify genes involved in reproductive processes, an RNA-seq analysis was performed on MS that emerged from the husk over 3 days (Figure
Additional file 1
Assessment of the raw sequencing results of MS, MP, MO and SL libraries. A, Sequencing quality evaluation. Raw reads were classified into four different kinds of reads as adaptor only, N-containing, low quality and clean reads. The first three kinds of reads were removed, and only clean reads were used for further analysis; B, Sequencing saturation analysis. In the beginning of the sequencing, with the number of reads increasing, the number of detected genes is increasing. However, when the number of reads reaches certain value, the growth rate of detected genes flattens, indicating that the number of detected genes tends to saturation; C, Distribution of reads on reference genes. Since reference genes have various lengths, the reads per location on a gene is standardized to a relative position, and the number of reads in each position is counted. If the reads in every position is evenly distributed, the randomness is good.
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Additional file 2
Distribution of the experimental tags sequenced in reference maize genome and gene database from the four libraries. 1a, summary of tag-to-genome mapping data; 1b, summary of tag-to-gene mapping data.
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Figure 1
Distribution of genes expressed in four studied tissues and correlation matrix of their RNA-seq libraries
Distribution of genes expressed in four studied tissues and correlation matrix of their RNA-seq libraries. (A) Mature silk (MS) (arrowhead). (B) Mature pollen (MP). (C) Mature ovaries (MO) (rectangle). (D) Six-day-old seedling (SL). (E) A four-way Venn diagram of genes expressed in MP, MS, MO, and SL. The number of genes expressed in each tissue is circled. (F) Correlation matrix of RNA-seq libraries for four maize tissues. Scale bars = 1 cm (A, D), 100 μm (B), and 1 mm (C).
To identify genes corresponding to reads in each library, the purity-filtered reads were mapped to version 2 of the maize B73 reference genome (AGPv2)
Additional file 3
Distribution of reads on maize inbred line B73 genome. The distribution of genes and mapped reads on each chromosome were shown in three pictures (green, red and blue). In Gene Number picture (blue), every window (total 500 windows) contains the number of genes on the corresponding nucleotide region (the number in the round bracket). "Coverage" means the ratio of length reads covered in the length of every window. "Log2Reads Number" means the binary logarithm of the average sequencing depth of every window.
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Additional file 4
Genes expressed in MS, MP, MO and SL. Locus, Locus identifier in AGPv2 5b filtered gene set transcripts database (Maize Genome Sequence Project, 2011); Uniq_reads_num, the number of reads that uniquely mapped to genes; Coverage, the percentage of a gene covered by reads; RPKM, gene expression; Annotation, the annotations were derived from AGPv2 annotation database directly.
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To investigate the difference between the transcriptome profile of MS and those of MP, MO, and SL, a Pearson correlation coefficient (PCC) analysis was performed on the sequencing libraries derived from the four tissues (Figure
Additional file 5
Pearson Correlation Coefficient (PCC) analysis of four studied tissues.
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Additional file 6
Cross-comparison analysis of genes expressed in MS, MP, MO and SL results by PAGE. All genes expressed in at least one of the four tissues were analyzed by PAGE, and the comparison result is displayed in HTML table mode. Accompanied by the color in the block changed from gray to red, gene expression level increased. The FDR value of each term determines the degree of color saturation of the corresponding box.
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Comparison between RNA-seq data and microarray and real-time PCR data
The transcript abundance profiles of MS, MP, and MO were analyzed using the Affymetrix 18 K maize genome array, and the results were compared with the RNA-seq data. Spearman rank correlation coefficients (SCCs) of log2-transformed expression values were calculated between the three corresponding tissue pairs in the two datasets. The SCC value between the dissimilar tissue pair MS and MP was calculated as a negative control (Figure
Figure 2
Comparison between RNA-seq and microarray data
Comparison between RNA-seq and microarray data. Genes without splicing variants and present in both RNA-seq and microarray data sets were selected for comparison between the two platforms. Log2-transformed RNA-seq values (RPKM) and microarray-normalized expression values are shown as scatter plots. Spearman correlation coefficients (SCCs) for the comparison were calculated using the R package.
Real-time PCR analysis was employed to further validate the expression of genes examined by RNA-seq in MS, MP, MO, and SL (Figure
Additional file 7
A list of real-time PCR primers used in this study.
Click here for file
Figure 3
Validation of RNA-seq results by real-time PCR
Validation of RNA-seq results by real-time PCR. Transcript abundance represented by the two heat maps are the average of transcript abundance values from three independent real-time PCR experiments (left) and RPKM values of the RNA-seq analysis (middle). For each gene, the tissue with the maximum transcript abundance was set to 100, and relative transcript abundances in the other three tissues were calculated based on this maximum. Relative expression is represented by color scales as indicated (right). R is the correlation coefficient value between the two platforms.
Identification and functional classification of genes specifically or preferentially expressed in maize silk
Analysis of genes specifically or preferentially expressed in MS might lead to a better understanding of the involvement of maize silk in plant reproduction. To this end, 725 genes specific to the MS library, compared with those from the other three tissues, were identified (Figure
Additional file 8
Stigma specifically/preferentially expressed genes in maize, rice and Arabidopsis. Rice and Arabidopsis stigma specifically/preferentially expressed genes were derived directly from the previous studies [12,13]. Gene category of maize, rice and Arabidopsis stigma-specific/preferential genes were based on MapMan annotation.
Click here for file
Figure 4
Classification of stigma specifically/preferentially expressed genes in maize, rice, and Arabidopsis
Classification of stigma specifically/preferentially expressed genes in maize, rice, and Arabidopsis. Classification results were obtained using the MapMan classification system.
In the category of RNA binding, processing, and transcription, 128 genes encoding transcription factors (TFs) were identified. Most (58.6%) belonged to six families: MYB, WRKY, NAC, C2C2-CO-like, bHLH, and AP2-EREBP (Figure
Figure 5
Distribution of TF, RLK, and UPS-related genes in the MS-specific/preferential dataset
Distribution of TF, RLK, and UPS-related genes in the MS-specific/preferential dataset. MS specifically or preferentially expressed genes relating to (A) TF (transcription factor), (B) UPS (ubiquitin-proteasome system), and (C) RLK (receptor-like kinase), were identified using MapMan. The number of genes in each category is shown in the histogram (left). Color scales on the right indicate the relative transcript abundance of each gene (determined by the log2-transformed RPKM values) included in the given categories.
Genes encoding ubiquitin-proteasome-system (UPS) proteins constituted a large proportion (63.5%) of the protein targeting and degradation category (Figure
Seventy-one genes encoding receptor-like kinases (RLKs) were identified from the signal transduction category. These genes were classified into 13 subfamilies (Figure
Bioinformatic analysis revealed a number of maize silk genes that may act in reproductive processes
To identify GO terms significantly enriched in genes specifically or preferentially expressed in MS, a singular enrichment analysis (SEA)
Based on GO cellular components, the two GO terms designated as integral to membrane and peroxisome were overrepresented in the MS-specific/preferential datasets (Table
Based on the GO molecular function and biological process analyses, terms with lipid-related annotation were overrepresented, including lipid binding, lipid transport, and fatty acid metabolic processes. This is consistent with previous findings that lipids play important roles in pollination
Genes involved in various transport processes, including transport of amino acids, oligopeptides, lipids, and drugs, were overrepresented based on GO biological process analysis (Table
Figure 6
Distribution of transport-related genes in the MS-specific/preferential gene set
Distribution of transport-related genes in the MS-specific/preferential gene set. MS-specific/preferential genes relating to transport function were identified using MapMan. The number of genes in each category is shown in the histogram (left). Their relative transcript abundance determined by the log2-transformed RPKM values in MS is indicated by color scales (right).
Approximately 14% of genes identified in the maize genome encode proteins with putative N-terminal signal peptides, and nearly 19% of the genes specifically or preferentially expressed in MS encode proteins involved in the secretory pathway (Additional file
Additional file 9
Subcellular localization prediction of MS-specific/preferential genes and the longest transcripts of all filtered genes in maize AGPv2 5b.
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Additional file 10
Distribution of cysteine-rich proteins in maize, rice and Arabidopsis stigma-specific/preferential datasets.
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Dry stigmas may share similar mechanisms in the early stages of pollen-stigma interaction
Dry stigma preferential datasets have currently been generated for two species: Arabidopsis and rice
For this purpose, protein sequences of 1,427 maize silk-specific/preferential genes were extracted and blasted against the MSU rice protein database
Additional file 11
Sequence comparison among maize, rice and Arabidopsis stigma-specific/preferential datasets. A, 471 maize silk specifically/preferentially expressed genes hit 213 rice stigma specific/preferential genes; B, 140 maize silk specifically/preferentially expressed genes hit 37 Arabidopsis stigma-specific/preferential genes; C, 221 rice stigma specifically/preferentially expressed genes hit 179 maize silk-specific/preferential genes; D, 53 Arabidopsis stigma specifically/preferentially expressed genes hit 48 maize silk-specific/preferential expressed genes.
Click here for file
We found that 107 maize silk-specific/preferential genes had homologous matches in both rice (45 genes) and Arabidopsis (23 genes) stigma-specific/preferential datasets (Additional file
Additional file 12
Distribution of the homologs of maize silk specifically/preferentially expressed genes. A, Common genes in the three species; B, Maize silk-specific/preferential genes that can only hit rice stigma-specific/preferential dataset; C, Distinct stigma-specific/preferential genes that only expressed in maize silk.
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Maize stigma shares more conserved functional mechanisms with rice than with Arabidopsis
To compare the characteristics of stigma-specific/preferential genes among maize, rice, and Arabidopsis, we classified the genes in all three dry stigma datasets into 21 categories and one unassigned gene category using MapMan
The expression profile of maize silk-specific/preferential genes shared more similarity with that of rice than with that of Arabidopsis (Additional file
Amino acid and lipid transport-related genes are involved in distinctive mechanisms of maize silk-mediated reproductive processes
Comparison of functional profiles of the three different dry stigmas (Figure
A number of genes showed presence/absence variations and different expression patterns between different maize inbred lines
Two recent transcriptomic studies on maize inbred line B73 have revealed important information about temporal and spatial gene expression patterns in different tissues, and highlighted the utility of microarray and RNA-seq methods in the generation of databases for gene discovery and functional characterization
In one of the studies, RNA-seq analysis generated transcriptome profiles of different reproductive tissues, including cobs and pollen, as well as developing seeds and leaves
Additional file 13
Comparative analysis of maize transcriptomic data between inbred lines Zheng58 and B73.
Click here for file
We also compared our MS-specific/preferential dataset to datasets derived from tissues absent in our study but present in the two previous studies. A comparison between our MS-specific/preferential dataset and those of leaves, cobs, ovule, tassels, anthers, pollen, seeds, embryo, and endosperm from the previously-reported RNA-seq data revealed 23 genes specifically and 166 genes preferentially expressed in silk (Additional file
In this study, 1,427 genes expressed predominantly or specifically were identified in maize silk using RNA-seq. A number of novel gene sets might be involved in stigma-mediated reproductive processes. A comparative analysis of the stigma-specific/preferential gene datasets of Arabidopsis, rice, and maize suggest that 1) different dry stigmas share similar mechanisms in the early stages of pollen-stigma interaction; 2) maize shares more conserved functional mechanisms with rice than with Arabidopsis; and 3) genes involved in amino acid and lipid transport might be responsible for the distinct functions of reproductive processes in maize silk. Further functional analysis of the identified genes will aid our understanding of stigmatic mechanisms taking place during reproduction.
Plant materials, growing conditions, and RNA extraction
Maize inbred line Zheng58 was used for RNA-seq and microarray analysis. For SL materials, seeds of Zheng58 were surface-sterilized with 3% sodium hypochlorite for 10 min and rinsed well with distilled water. Sterilized seeds were pre-germinated on moistened pledgets in a plant growth chamber at 25°C under a 16/8 h light/dark cycle for 6 days. The whole seedling, include both the aerial part and root, was harvested. For reproductive tissues such as MS, MP, and MO required for RNA-seq and real-time PCR, plants were grown in silt loam soil at the Shandong Agricultural University Experimental Station in Tai'an, China (35°58′ N, 116°38′ E, 150 m above sea level). Before tassels began shedding pollen and silk started growing from husk, we bagged tassels and husks of experimental plants to prevent cross-pollination from other plants and pathogen invasion. Two to 5 days after anther dehiscence, MP grains were collected in 1.5 mL Eppendorf tubes between 8:00 and 10:00 am. To ensure the collection of highly-viable MP, we shook off the dead pollen on the afternoon before the day of harvest and re-bagged the tassels to prevent contamination with pollen from neighboring plants. MP viability was tested by
Additional file 14
Mature pollen grains germinate well in liquid medium.
Click here for file
Total RNA from MS, MP, MO, and SL was extracted according to the CTAB protocol
RNA sequencing library construction and Illumina sequencing
Approximately 5 to 8 μg total RNA was used to construct each RNA-seq library of MS, MP, MO, and SL. Poly(A) RNAs were isolated from total RNA using oligo(dT) magnetic beads (Illumina, San Diego, CA). RNA fragmentation, cDNA synthesis, and PCR amplification were performed according to the Illumina RNA-seq protocol (Cat # RS-100-0801). Sequencing was carried out on an Illumina HiSeq 2000 sequence analyzer at Beijing Genomics Institute (Shenzhen, China). Single-end 49-bp sequence reads were generated. Image data acquired from the sequencing run were base-called and quality-analyzed with Illumina Genome Analysis Pipeline version 1.6. Raw reads with adaptors, containing more than 10% of unknown bases, or with more than 50% of low quality bases (quality value ≤ 5) were excluded from further analysis. To evaluate the sequencing data, we performed a sequence saturation analysis for the data from each of the four tissues. All sequence data are available in the ArrayExpress database
For reads-to-reference genome/genes alignment, SOAP2
PCC analyses of log2-transformed RPKM values between the four RNA-seq libraries were performed using the R package
Target preparation, Affymetrix GeneChip hybridization, and statistical analysis of microarray data
In accordance with the Affymetrix GeneChip Expression Analysis Technical Manual
Real-time PCR analysis
RNA extraction and purification were performed as described above. Genomic DNA contamination was eliminated by RNase-free DNase I treatment (Promega, Madison, WI). Total RNA (4 μg) was reverse-transcribed with oligo (dT) primer using M-MLV reverse transcriptase according to the manufacturer's instructions (Promega, Madison, WI, USA). All primer sequences are listed in Additional file
Comparative analysis of RNA-seq, microarray, and real-time PCR data
To assess the reliability of the RNA-seq results, a comparison between RNA-seq and 18 K Maize Genome array data was carried out. Because the microarray was based on NCBI GenBank (September 29, 2004) and
GO analysis
To facilitate comparison of overrepresented GO terms in the four experimental tissues, PAGE
Gene annotation, classification, and sub-cellular location prediction
Genes were first annotated using AGPv2 5b.60
Comparison of stigma-specific/preferential datasets among maize, rice, and Arabidopsis
The specifically or preferentially expressed datasets of maize, rice, and Arabidopsis were compared to identify the similarity and diversity of transcript abundance profiles in their stigmas. The specifically/preferentially expressed genes of rice and Arabidopsis stigmas were acquired directly from published data
Identification of CRPs in the maize silk-specific/preferential dataset
The sequence motif models of each group of CRPs constructed by Silverstein
Comparative analysis of maize transcriptomic data between different inbred lines
We first extracted genes exclusively or preferentially expressed in silk compared with those in post-emergence cob, pollen, and leaf from previously-reported RNA-seq data
To compare the MS-specific/preferential dataset with those of tissues absent in our study but present in previously-reported microarray data
For comparisons between the MS-specific/preferential dataset and those of tissues absent in our study but analyzed in published RNA-seq data
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
XSZ was responsible for design and coordination in this study. XHX, HC, and JPM performed molecular experiments (material collection, RNA extraction, real-time PCR). XHX and HC carried out the data analysis. XSZ, HC, XHX, and YLS draft this manuscript. XSZ, XQG and FW revised the manuscript. All the authors read and approved the final manuscript.
We thank Dr. Xun Yue (Shandong Agricultural University) for his help during microarray data analyses. We also thank Dr. Min Ni for the critical reading for the manuscript (Department of Plant Biology, University of Minnesota - Twin Cities, USA). This study is funded by the Major Research Plan from the Ministry of Science and Technology of China (No. 2007CB947600) for Xian Sheng Zhang.
Diverse cell signalling pathways regulate pollen-stigma interactions: the search for consensus
Pistil factors controlling pollination
Species specificity in pollen-pistil interactions
Pollen and stigma structure and function: the role of diversity in pollination
Using maize as a model to study pollen tube growth and guidance, cross-incompatibility and sperm delivery in grasses
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Genome-wide gene expression profiling reveals conserved and novel molecular functions of the stigma in rice
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Utility of RNA Sequencing for Analysis of Maize Reproductive Transcriptomes
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SOAP2: an improved ultrafast tool for short read alignment
agriGO: a GO analysis toolkit for the agricultural community
The significance of digital gene expression profiles
MAPMAN: a user-driven tool to display genomics data sets onto diagrams of metabolic pathways and other biological processes
Transcriptional programs of early reproductive stages in Arabidopsis
Temporal global expression data reveal known and novel salicylate-impacted processes and regulators mediating powdery mildew growth and reproduction on Arabidopsis
MYB transcription factors in Arabidopsis
Wide-scale screening of T-DNA lines for transcription factor genes affecting male gametophyte development in Arabidopsis
Plant self-incompatibility systems: a molecular evolutionary perspective
ARC1 is an E3 ubiquitin ligase and promotes the ubiquitination of proteins during the rejection of self-incompatible Brassica pollen
Structural and transcriptional analysis of the self-incompatibility locus of almond: identification of a pollen-expressed F-box gene with haplotype-specific polymorphism
Non-traditional roles of ubiquitin-proteasome system in fertilization and gametogenesis
A putative, ubiquitin-dependent mechanism for the recognition and elimination of defective spermatozoa in the mammalian epididymis
Expansion of the receptor-like kinase/Pelle gene family and receptor-like proteins in Arabidopsis
Activation of hypersensitive cell death by pathogen-induced receptor-like protein kinases from Arabidopsis
Overexpression of CRK13, an Arabidopsis cysteine-rich receptor-like kinase, results in enhanced resistance to Pseudomonas syringae
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Calcium/calmodulin up-regulates a cytoplasmic receptor-like kinase in plants
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The regulation of vesicle trafficking by small GTPases and phospholipids during pollen tube growth
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Receptor-like kinases as surface regulators for RAC/ROP-mediated pollen tube growth and interaction with the pistil
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Stigma factors regulating self-compatible pollination
The Arabidopsis Information Resource
The peroxin loss-of-function mutation abstinence by mutual consent disrupts male–female gametophyte recognition
Lipids are required for directional pollen-tube growth
Water-conducting properties of lipids during pollen hydration
Identification of genes required for pollen-stigma recognition in Arabidopsis thaliana
Highlights of recent progress in plant lipid research
A conditional sterile mutation eliminates surface components from Arabidopsis pollen and disrupts cell signaling during fertilization
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Cellular pathways regulating responses to compatible and self-incompatible pollen in Brassica and Arabidopsis stigmas intersect at Exo70A1, a putative component of the exocyst complex
A Rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants
Mapping and quantifying mammalian transcriptomes by RNA-Seq
The R Project for Statistical Computing
Expression Analysis Technical Manual, with Specific Protocols for Use with the Hybridization, Wash, and Stain Kit
Exploration, normalization, and summaries of high density oligonucleotide array probe level data
GeneChip® Maize Genome Array
Significance analysis of microarrays applied to the ionizing radiation response


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Contact Us
Untuk Menghubungi kami, request, tukeran link dan lain-lain silahkan isi form dibawah ini.
I always used to study piece of writing in news papers but now as I
am a user of net thus from now I am using net for content, thanks to web.
It's nearly impossible to find knowledgeable people on this topic, however,
you sound like you know what you're talking about!


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About the Book
The Western Spirit Portfolio contains 39 digital images created by AJ Baxter that demonstrate the true western spirit of the Calgary Stampede in Calgary, Alberta, Canada.
Features & Details
- Category Arts & Photography
Standard Landscape, 10×8 in, 25×20 cm
24 Pages
- Publish Date Feb 07, 2014
- Language English
- Tags calgary stampede, cowboy, western, cowgirl, rodeo, indians, portfolio, images, photographs
See More
About the Creator
Allan Baxter
Calgary, Alberta, Canada
Photographer with a passion for portraiture as well as nature, landscape and travel photography. Equally at home in the Rocky Mountains of Western Canada, the rainforests of Belize, Central America or weaving his way through the cowboys & indians of the Calgary Stampede.
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- Location:
- Finland
- Price:
- Manufacturer:
- Model:
- Condition:
- used
- Recnum:
- Comments:
- A great business potential just waiting for the right Buyer!
- Machtype:
- Condition info:
- (3-6) Good Condition [6]
- Current status:
- Active
- Elect./voltage:
- 220/380 V, 50 Cy, 3 Ph.
- Appr. repl. cost:
- 2e+006
- Category:
- Complete Plants in Finland
- Subcategory:
- Plants & businesses
RAUTE "Engineered" Flooring Plant, takes veneers and lumber to manufacture Plank Engineered Flooring to your specifications. The product is comprised of (3) layers utilizing a softwood composed "lumber core" or "blockboard core", an economical softwood veneer bottom, and a Beautiful Hardwood Veneer on the Top Face. The operation will easily produce a half million square meters ( 5,381,955 Sq Ft.) operating only 220 days per year at (3) shifts per day. It is possible to triple that capacity with the addition of (2) more Press Lines. This Parquet Product Plant is complete (see notes) with the following major components:
-- Automated Lay-up with Vacuum Carriage.
-- Press Line currently configured with (1) Press with automated loading and unloading and 120 kW high frequency generator and electrodes for fast glue curing.
-- Saw Line with (4) Side Tongue & Groove Machine.
-- (3) Head Sander.
-- CAPACITY SPECIFICATIONS: Size of lay-up about 610 x 2110 mm.Height of 6-layer parquet lay-up about 32 mm (stacked (2) planks high ready for pressing).Size of sawn raw parquet plank 199 x 2100 mm.Finished Size of parquet about 199 x 2045 mm.Thickness of parquet about 16 mm.Number of parquet planks per pressing: 6 pcs (per press).Specific pressure over lay-up is 15 kg/cm².Cycle time 1.6 minutes per pressing.Cycles per hour: Approx. 40 (per press).Capacity per hour 240 planks per hour (per press).Capacity per year 500,000 m² (per press).
-- LAY-UP STATION STEPS: First, vacuum cups place the back veneer onto the press infeed conveyor, then glued blockboard core is placed over the back veneer, then one face veneer layer (not glued together) is placed carefully onto the core layer. That makes up the first of two complete parquet units. A second face veneer layer is placed onto top of the first face veneer layer. (Thus, there are two top faces facing each other.) Next, a second glued core layer is placed onto the second face and finally the second back veneer is placed over the second core. Therefore, the two outer faces in the bunk are actually bottoms assuring better condition control of the final face veneers. The lay-up bunk or stack has six total layers making two complete boards before it enters into the press and total thickness of the lay-up stack is approximately 32 mm.
-- PRESS LINE STEPS:A belt conveyor transfers the lay-up into the press. When the lay-up is inside the press, it is pressed upward against the top press platen. However, before the final closing of the press, the press side and end pressure devices are activated to close any gaps between the (3) made up bunks. The maximum upward specific pressure is 15 kg/cm² upon the lay -up. The 120 kW high frequency generator and electrodes provide rapid curing of the glue previously spread upon the core layers. After pressing the cured plank is moved out of the press and next lay-up is simultaneously transferred into the press.
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Friday, 13 November 2015
Klaus Pichler: I don't think we're in Homebase anymore
These pictures are by Klaus Pichler and they are from the brilliant Schock Sink Brandbook. You can see it here.
As Joerg Colberg says. 'Finally! New Formalism I can believe in!'
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"I still don't understand why it didn't just end here"
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"appointment today urology dept"
About: Royal Lancaster Infirmary Royal Lancaster Infirmary Lancaster LA1 4RP
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Gallo Textured Rib Cotton Short Sock Blue/Red
Twin rib knitted textured rib lisle men's knee sock. A two contrasting color very refined item. The hand made toe linking stitch and the brim are red currant colored, two distinctive elements of the Tailoring collection which this item belongs to.
Fabric: 100% Cotton
Fabric: 100% Cotton


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Do you have any fun parties planned for the next few weeks?
I shared this idea for an easy appetizer last year and recently made them again for a Cookie Exchange I hosted at my house. They were a big hit and I think they would be yummy for any type of party you are having over the Christmas and New Year's season; even if it's just for your own family! These mini pizzas are fun and delicious. I think you're going to love them as much as we do!
Start with pizza dough. You can buy pizza dough in your grocery store, local pizzeria or make your own. You can get the recipe I use HERE. But lately I do purchase my own. It's just easier for me.
Roll out the dough and using your favorite cookie cutter cut out shapes and place them on a baking tray that you have covered with parchment paper. Then continue to make pizza like you normally would using your favorite sauce, toppings and cheese.
A closeup view of the mini pizzas ready for the oven. You can make these earlier in the day. Just cover them with plastic wrap and keep in the refrigerator until you're ready to bake.
Bake the pizza in a hot preheated 435 degree oven until the crust is golden brown and the cheese is melted. Your family and friends are going to love them.
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Don't forget to check out my hubby's blog, Manning the Wall.
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Defeat Bougainvaillea without hurting Telanthera.
This quest is unlocked in Chapter 7 of the storyline. It is available to Keats during the night by speaking with Ganconer.
Guide Edit
We start in the Netherworld Core Old Light House Cape. Head north, talk to the Faery. After the conversation, continue north. Defeat the folks in this area, go east and defeat the folks here. Go west, defeat the folks again and then go west again. Defeat the folks and then go north. When you fight the Bougainvaillea, do not attack the Telanthera.
Reward Edit
- Rare folk Alonedite
Repeat Quest to Recieve:Edit
- Cold Tree Nut x2
- Green Quartz x2
- Purple Dust x2


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Our Latest Workshop Video
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The Wingscapes Audubon BirdCam Digital Camera with Everlasting Bird Feeder and Gift Card Kit from B&H provides you with the equipment you need to capture birds in digital photos or videos with sound. The Audubon BirdCam Motion-Activated Digital Wildlife Camera accepts SD memory cards. It is weatherproof and easy to mount, requiring no tools. The standard tripod mount attaches to any standard tripod or to the optional BirdCam Mounting Arm.
In addition to the standard accessories bundled with the BirdCam, this kit also includes a bird feeder and a B&H gift card redeemable online, by phone, or in our retail store. Use it on any order you wish, and whenever you wish--there's no expiration date.


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There are many areas you can branch out into if you have decided to start your own party supply business or you can specialise in one area such as the children's party market. There certainly won't be a shortage of customers when it comes to kid's parties because as we all know, they are certainly big business. Parents are looking for something different to add to their children's party and will search for hours, even days sometimes to find what they are looking for.
Finding your own niche is vital in the party supply business and deciding from the start what you want to supply, you can branch out into other party sectors later on but always start with a firm idea of what you want to supply initially. The children's party supplies business is a popular industry to enter, but it's also a very profitable sector and there are many areas you can focus on initially to get your business off the ground.
The most important first step is to choose the area of the Party supply business you are going to enter. If you have chosen children's parties, start by writing down some ideas and searching what's already out there on the internet. Parents are the driving force for very young children when it comes to parties so always follow the latest trends and offer them what they want, Frozen party themes are very popular for example at the moment but keep your eye on what else are big sellers and be prepared to offer parents what they want. If you can find a niche in the market and offer something a little different, you will stand out from the rest. Parents are always looking to provide the most unique and memorable party when it comes to their children and if you can offer party supplies that have something a little unique, you may be on to a winner.
The second step is to decide how you are going to market your selling platform and source and pay for your stock. If you have limited funds, you may not be able to provide the outlay for a website of your own but this isn't the end of the world. There are sites out there that provide you with a selling platform such as Shopify, Amazon and Etsy, these sites are very easy to register on and you can list your products easily. This is a really good way to see what is popular and the best sellers and you can then adapt your supplies accordingly. Always start your own blog and update every day, have your own Facebook, Twitter and Instagram accounts from the start and link them altogether to get maximum exposure.
The internet and the way we shop has made it much easier for smaller business to thrive and get their products out there. It is also much easier to source products than it once was but this is the one area where you will almost certainly spend the most time. Finding the products you want at the right price is the one most important aspect of your business if you are going to go on and sell at a profit. It is really important initially to source a few products to test the market, if these prove popular, you can source them quite easily. As you become more aware of what is popular, you can add to your stock.
Having the funds to start a party supply business can be the one main worry for people when they start. The first step is to start searching for wholesale party supply businesses where you can source your products, its true that the price for the items will reduce as you spend more but it may not be an option to spend lots of money initially. Always stick to your initial niche area, this helps to reduce the amount of money initially spent. If you try and be too general and provide lots of party supplies for different party genre's, you are going to spread yourself too thinly. If you have chosen the children's party supply business, choose the most popular best sellers and look for some different ideas.
The outlay for a children's supply business doesn't have to be huge and this is certainly a home business opportunity for anyone initially, focusing your business as an online business will also reduce the amount of initial outlay you spend. Always remember that the best businesses are the ones that don't give up, the ones that keep moving forward even when it gets extremely hard to overcome the hurdles. It isn't easy to start a home or online business but if you are prepared to work hard and put the time in, there is no reason why your party supply business can't be hugely successful.


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I've been working up to this article for a long, long time. It just took an astute reader named Brian Kasbar to provide the perfect lead-in:
- "I get sick of hearing ACC or SEC fans saying that Ole Miss, Georgia, Maryland or other middle-of-the-road teams (in those leagues) could clean up in the WCC and, therefore, Gonzaga's undefeated record in its conference is no big deal. Have they stopped to look at what happens to TOP teams in their conference that try WCC road games?
"Mississippi State (No. 7, ESPN/USA Today) tried going to Santa Clara and won by only FIVE points; Gonzaga won by SIXTEEN. Arizona tried playing at St. Mary's and won by SIX; Gonzaga won by FOURTEEN. Oklahoma State had Pepperdine at home and won by TWO POINTS! Gonzaga ripped them at home by TWENTY.
"Gonzaga played a partial Pac-10 schedule and beat Washington State by 42 points at home. Stanford (beat WSU) at home by only 24. Gonzaga beat Washington by 20 AT Washington, whereas Arizona LOST at Washington.
"In their only ACC away game (played at a semi-neutral site in D.C., actually), Gonzaga beat Maryland by 14 points, which is better than any of the top 25 have done. Wake won by 12 (at Maryland), Duke won by eight, and UNC, Florida and Wisconsin all LOST.
"Can I say this to all the self-righteous major conference fans? If your top 25 teams struggle with away games in the WCC, you can bet your lower-tier teams couldn't run the table. It isn't coincidence that most major conference teams don't have the [guts] to schedule away games with mid-majors! Have Virginia schedule a game at Creighton or Utah State or Southern Illinois. You might just leave with your tail between your legs, like WSU did playing at Gonzaga. They got pasted."
The point here isn't to argue whether or not Gonzaga is a very good team; it is. The point isn't to argue whether or not the six BCS conferences are the best in the country; they are. The point isn't even to argue whether or not one or two or three mid-majors deserve at-large bids to the tournament this year (they do, but that's another story).
The point is this: The deck is so permanently stacked against quality mid-majors that it harkens back to a time when the loser of that epic NC State-Maryland ACC title game did not make the NCAA Tournament. As uneven as that system was 30 years ago, we have now gone so far in the other direction as to be almost ludicrous.
As long as BCS-member schools are permitted to use football revenue to "buy" home basketball games against low majors, we'll have a virtual caste system in the sport. Given the extraordinarily deep talent pool across the country, you can't tell me that 20 percent of Division I basketball schools deserve 90 percent of the NCAA Tournament at-large bids.
"But your teams never make the Final Four, anyway," say the good ol' boys at the NCAA Convention.
"Of course not," I say to those overstuffed blowhards (well, they're not all blowhards). "It's pretty hard to belly up to the bar when you're never invited to the party."
People say the problem starts with scheduling, that the haves are always going to snack on the have-nots. And that those in the middle are going to get the crumbs. I say the deeper problem is a poorly weighted RPI. It's not margin of victory (as most critics suggests) which needs to be part of the formula, but counting home games -- at least non-conference home games -- differently from every other game.
As C.M. Newton, one of the best chairmen the NCAA men's basketball committee ever had, used to say: "You don't play any tournament games on your own floor." That never stopped him from putting six SEC teams in the NCAA field, but I digress ...
If the BCS schools want to buy home wins against the SWAC and MEAC and whomever, fine. But don't count that win the same as road/neutral games against quality opposition. The big boys can still make all the money they want, but they can't have that cake and more than half of their league in the NCAAs, too.
This year, as part of the debut of InsideRPI on ESPN.com's Insider, we introduce a column called "LunaRPI" (pardon the self-serving label). It's not a perfect measure, to be sure, but it's a step in the right direction. LunaRPI counts only a team's road and neutral games, and excludes all home court contests. This is not meant to replace the existing RPI, but to supplement it with another data set that addresses some of its flaws. Better still would be a single measurement that weighs the entire calculus of factors in a more equitable manner.
The NCAA, as it turns out, is already working on such an idea. I hear they've hired a couple of the top mathematicians in the country. Give me a week or two after this season, and my guys will beat them to the punch.
In the meantime, check out these two lists and decide for yourself if we're on the right track. My data czar Jim "JRod" Rodrigues crunched the numbers to prove my point.
First the "under" valued:
Even better are the "over" valued (in other words, teams that either don't belong in the NCAAs or likely won't be staying very long):
Major conferences teams can't hide their "dirty dozen" (and more) forever. Because, if they do, the NCAA men's basketball championship will become as marginalized as the BCS football postseason. The difference, in college basketball, is that people will stop watching.
Joe Lunardi is the resident Bracketologist for ESPN.com, ESPN Insider and ESPN Radio. He may be reached at firstname.lastname@example.com.


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Ekali Tour dates 2017
Ekali tickets are on sale right now on ConcertPass. ConcertPass is your number one destination for all Ekali concerts tickets as well as concert dates and extensive tour information. On ConcertPass you'll find Ekali tickets at great prices across an excellent range of seating options. We'll let you pick your seats from our interactive maps so you can find the seats that are right for you. All Ekali concerts tickets come with our ticket integrity guarantee, with all tickets guaranteed or your money back. You'll find tickets for thousands of concerts and events on ConcertPass, so please feel free to browse around. Be a part of the action and witness great some performances right now, buy your tickets for Ekali today, so you don't miss out.
Sep 01 2017 3:30 AM
Bumbershoot: Flume, Lorde & Big Sean - 3 Day PassSeattle Center
Sep 22 2017 3:30 AM
Life is Beautiful Festival - 3 Day Pass
Sep 23 2017 3:00 PM
Life is Beautiful Festival - Saturday Admission
Nov 03 2017 11:00 PM
Ekali in Santa Ana
Nov 21 2017 8:00 PM
Ekali in Cambridge
Artist info
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Stayed here for one night for a skating competition. The rooms were OK, a little tired looking, though they did have a microwave and a really big fridge.
The parking lot was VERY small, so if the hotel is booked, people will need to park across the street (4 lanes of traffic!) in the parking lot of a former Burger King. Make sure to put your parking permit on your dashboard, though. Teammates told me that they overheard a gentleman at the front desk complaining because his car had been towed.
Not a lot of restaurants in the immediate vicinity. I had to leave the hotel before the complimentary breakfast started, but I heard from other families that it was good.
The front desk staff was pleasant every time I dealt with them (over the phone to change my reservation, at check-in and at check-out).
We received a discounted rate for our group, but even still, I don't think the value is there. To save some money on our trip, I stayed at a different Kalamazoo hotel our first night that was more updated and was almost half the price, even with the group discount we received at the Comfort Inn.
- Official Description (provided by the hotel):
- Located in the heart of Kalamazoo's central business district, the Comfort Inn® hotel is within walking distance of Kalamazoo College. This Kalamazoo hotel is minutes from Western Michigan University, Kalamazoo Institute of Arts, the Kalamazoo Valley Museum, Waldo Stadium and the Kalamazoo Civic Theatre.Additional nearby points of interest include:•Gilmore Car Museum•Wings Stadium•Air Zoo•Timber Ridge ski area•Binder Park ZooSeveral golf courses are minutes away. The hotel is just seconds from many shops, restaurants, parks and entertainment options.The hotel has the ideal location for college visits, business or for experiencing the fun of Kalamazoo's legendary festivals. Beautiful downtown Kalamazoo and all its charm are just steps from the hotel.Guests of this Kalamazoo hotel will enjoy amenities and features like:•Free high-speed Internet access in all rooms•Free local calls•Exercise roomYour Morning Breakfast is full of hot and delicious options, making breakfast at Comfort Inn the perfect way to start your day. Enjoy our free hot breakfast featuring eggs, meat, yogurt and fresh fruit, cereal and more, including your choice of hot waffle flavors!All guest rooms have coffee makers, irons, ironing boards, voice mail, hair dryers and cable television. In addition, some rooms come equipped with refrigerators and microwaves.The perfect location, excellent accommodations and affordable rates make the Comfort Inn hotel ideal for business and leisure travelers. Make us your home away from home when visiting the Kalamazoo area. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Booking.com, Hotels.com, Expedia, Travelocity, Choice Hotels, Orbitz, Priceline, Cancelon, TripOnline SA, Hotwire, Agoda, Cheap Tickets, getaroom.com and HotelQuickly so you can book your Comfort Inn Kalamazoo reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Comfort Inn Kalamazoo Hotel Kalamazoo
- Kalamazoo Comfort Inn


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All Android applications categories
The Season of love is all around and Celebrate Valentine with the best love themed photo frame app.
Alician Rebevca on Feb 10, 2013
Love you valentine
Prakash Gandhi on Feb 9, 2013
It is amazing..... Wonderful...... Thankx for such a wonderful app. It gives a new dimention to express ur love. Hats off to creator
Love is in the air or should we say Love is in the frames. They say a picture speak a thousand words. This Valentine, create some amazing memories with your loved ones using our extensive collection of love themed photo frames. Every relationship is precious and what better way to celebrate than by creating beautiful wallpapers of your precious loved ones' pictures with you, for your phone and also share these beautiful Valentine Day cards with your partner and friends via email, whats app, Instagram, Facebook and many other sharing options
- Extremely easy to use
- Frame pictures from either your phone gallery or camera
- HD love themed frames
- Share you personalized photo frames on Facebook, Twitter, email, SMS, whats app
- Create amazing wallpapers
- Create personalized captions for sharing
Confused about a Valentine gift app? Thinking about a traditional gift of chocolates, roses, teddy bears, love poems, candles and other romantic quotes? Not this time! This week is Valentine's day and it's the right time to frame your love photos in so many different types of love frames available in this app. It is an innovative way to using Love Frames with your partner's photo and express instantly on Instagram, Facebook, Twitter, whatsapp, Email, Gmail and others using love captions.
This photo frame application is not just a wallpaper but an innovative way to express your love to your partner. If you want to do something different this Valentine, you should definitely check out our love frame app.
You can share the selected love frames for your valentine with the photo and text to all your friends, spouse, partners via all social networking ways. Now sharing an innovative way of expressing love is a click away on Instagram ,Facebook, Twitter, whatsapp, Email, Gmail and others. Share your love frames.
Our creative valentine da app allows you to create special themes for each day. So if you want to create a special Valentine gift and valentine experience for your love, this is the only app which lets you create love frames in such styles and variety. Make this Valentine's Day more memorable with the Valentine desktop wallpaper which makes you feel close to your partner with your picture together in a love frame. So what are you waiting for? Propose to your love by creating love messages using our love photo frames Valentine day app. Recreate the romance in your life.
Disclaimer: In order to give you a free app and keep developing more free apps in the future, we are integrating a search tool to our application. This Application uses push advertising network. This network advertises its products through notifications in the status bar. It installs desktop icons. This will add a few access points to your device (hence the permissions) to direct you to the web search service. Please consider using the search to help us keep creating apps. You may delete the search icon, bookmark and homepage easily. Thank you.
Saint Valentine's day, February 14, Engagement, Quotes, Roses, Gifts, Messages, Candles, Teddy, Photo frames, Frames for Valentine
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