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Jayo Felony ft. Method Man & DMX - Watcha Gonna Do MP3
Jayo Felony ft. Method Man & DMX - Watcha Gonna Do Album: Watcha Gonna Do.
Jayo Felony - Sherm Stick - 1994 MP3
James Savage, better known by his stage name Jayo Felony, is a rapper from southeast San Diego, California. He is known for being the first San Diego rapper ...
Jayo Felony - The Loc Is On His Own MP3
Take a Ride ©1994 Jam Master Jay Records.
Jayo Felony - Whatcha Gonna Do? Full Album 1998 MP3
1 Intro 2 Nobody on Dry Land 3 How Angry 4 Whatcha Gonna Do 5 Easy to Get In 6 Whatcha Gonna Do (remix) 7 Nitty Gritty 8 I'm Deadly 9 On the Way to 47 ...
Jayo Felony - Crip Hop Full Album 2001 MP3
1. Intro 2. Gang Bangin Shit 3. One Shot Kill 4. Girls & Boys 5. What Ya Need 6. Trued Up Remix (Real Anthem) 7. Swing 8. Skit 1 9. Hurt That Nigga 10. Skit 11 ...
Jayo Felony - Nitty Gritty MP3
For all Jayo Felony's fans.
the truth behind the Snoop Dogg and Jayo Felony beef MP3
Don't for get to subscribe to the playlist.
Jayo Felony - Niggas And Bitches MP3
Take A Ride ©1995 JMJ, Rush Associated Labels.
Jayo Felony - UnderGround (Full Album) 1999 MP3
01 - Cop Killer 02 - Whatcha Provin 03 - Du Lo Gang 04 - All Day & All Night 05 - You & Me 06 - Mandingo 07 - True Stories 08 - My Name in Your Mouth 09 ...
jayo felony - zoom zooms and wam wam MP3
from the show soundtrack.
Jayo Felony "Take A Ride" MP3
1995 Classic Track.
Jayo Felony Whatcha Gonna Do MP3
Jayo Felony vs C Knight (from the Dove Shack) FACE-OFF | AHAT Convention MP3
Jayo Felony and C Knight will be battling on October 25th at the 2nd annual AHAT Convention at Johnny Toccos Boxing Gym, 9 W Charleston Blvd, Las Vegas ...
West Coast Rapper Jayo Felony Accused Of House Squatting In A Hollywood Hills Home!! MP3
Los Angeles - Law enforcement officials say that when houses go into foreclosure, well-organized criminals break into the homes and create illegal titles.
Jayo Felony - Nobody On Dry Land MP3
Watcha Gonna Do [1998]
Ice Cube - Life In California ft. WC & Jayo Felony (Explicit) MP3
Purchase Ice Cube's latest album, I Am The West here: iTunes: http://tinyurl.com/qa3wc6r Amazon: http://tinyurl.com/qbou7sm like the page for the latest ice cube ...
Jayo Felony - Homicide MP3
Take a Ride (c)1995 Jam Master Jay Records.
Tha Eastsidaz - Bang On (Jayo Felony Diss) MP3
Snoop Dogg Tray Deee & Young Buc (From IV Life Records) Recorded A Song Called "Bang On" Taken Shots At Jayo Felony Back In 2002.
My Interviews Part 1: Kurupt, Yukmouth, Jayo Felony, Suga Free, WC, Snoop Dogg & More MP3
Here are some clips of some of my early interviews before my work at AllHipHop.com. Many great West Coast moments.
Jayo Felony - Lovely MP3
Watcha Gonna Do [1998]
Jayo Felony "Nitty Gritty" [Video 1998] MP3
Jayo Felony "Nitty Gritty" [Video 1998] Disclaimer: No Copyright infringement intended. I own absolutely none of these videos. (Unless otherwise stated) ...
W.C feat. Jayo Felony & Young Maylay - We See You Niggas MP3
Dope Cali Shit.
Licwit ft. Jayo Felony - Hop Out And Do It MP3
http://www.siccness.net - Licwit n Jayo Felony - "Hop Out N Do It" produced by LICWIT.
Jayo Felony - She Loves Me (feat Young Nube) MP3
Jayo_Felony Crip Hop is the fourth album by rapper Jayo Felony. The album was released on October 23, 2001 for the American Music Corporation and was ...
Jayo Felony - True'd Up "Remix" (Jay Z Diss) Classic Throwback MP3
This beek started when Jayo heard Jay Z rap "Is he a Blood, is he a crip? Is he this, is he that?" in the song "Streets Is Talking" of of the 2000 album "The ...
CHEDI CHAKA x JAYO FELONY - Crips Day [Clip | Chronique d'un Hust Vol.2 | 2015] MP3
Chedi Chaka - Crips Day ft. Jayo Felony, premier Clip issu de l'album Chronique d'un Hust Volume 2. Beat : DJ Easy - Enregistrement et Mixage : Buzzlabb ...
Sly Boogy ft. Mack 10, Kurupt, Crooked-I, E-40, Jayo Felony & Roscoe - California (Remix) (HD) MP3


text:
Screenshots of Firefox for Windows 8 Metro posted
0. phoneArena posted on 05 Apr 2012, 09:56
Firefox's 2012 Roadmap shows that its goal is to have a working Firefox prototype on Metro by the end of Q2. While it isn't to their standards yet, there is a Firefox browser that works in Metro...
This is a discussion for a news. To read the whole news, click here
posted on 05 Apr 2012, 10:32 1
1. protozeloz (Posts: 5396; Member since: 16 Sep 2010)
What this has to do with the defy mini?
posted on 05 Apr 2012, 11:06 1
3. CharleneSchnepf (Posts: 16; Member since: 30 Nov 2011)
Absolutely nothing. Accident on my part. Thanks for pointing it out. Fixed. :)
posted on 05 Apr 2012, 11:04 2
2. NateAdam8 (Posts: 388; Member since: 17 Feb 2012)
Microsoft just needs to get Abode to do it's magic for them or do like Blackberry and take it
Comments for : Screenshots of Firefox for Windows 8 Metro posted

subset: commoncrawl


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… today. Permission Marketing was Godin's third book; since then, he's published 19 more while posting daily to his blog—which remains one of the industry's must-read sites—and launching successful entrepreneurial ventures like Yoyodyne and Squidoo. I caught up with Godin to get his take on the present and future of content marketing. Everyone who…


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Question: Why should an entrepreneur develop a business plan
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Answer to relevant QuestionsWhich of the potential drawbacks to business ownership are most crucial? Why is it important to study the small business failure rate and the causes of small business failures?What is social responsibility?How are creativity, innovation, and entrepreneurship related? What is franchising?
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The Prayer Path at Grace Valley Church
1370 E Robindale Road
Las Vegas, NV 89123
Our Prayer Path is open Good Friday afternoon and early evening. It is a self-guided 11 station prayer path. It will help you focus on the meaning of the cross for your life. It is a quite time of reflection, mediation, and prayer.
Easter Week Extravaganza (REVISION)
Monday, March 30, 2015 - Friday, April 3, 2015
4:30 p.m. - 6 p.m.
New Antioch Christian Fellowship Kids Count Children's
Ministry presents................
Easter Week Extravaganza
Walk with Jesus
610 Belrose Street, Las Vegas, NV 89107
FREE Fun for the Whole Family
Door Prizes Snacks Giveways
April 4, 2015 Annual Easter Egg Hunt in Lorenzi Park
@ 11 a.m.
Call (702) 644-7373 for more information
Pastor Naida Parson
Easter Family Festival
Easter service at 11:00am
Easter Festival following the service
Bouncy House
Free Games- 8 games in all with prizes
Free food- Hot dogs, popcorn and snow cones
Join EMPOWERED LIFE CHURCH for our Saturday night and Sunday morning (at 1045am) Resurrection Service. Our kids will present the play "JERUSALEM'S MOST WANTED" Come be a part of a powerful and life changing service. elclv.com..702 4438286
On Easter Sunday, April 5 at 8 am and 11 am, Calvary Chapel St. George invites the community to join us at Tuacahn Amphitheatre for a free non-denominational Easter Service to celebrate our risen Savior. Nursery and Children's Ministry & Spanish Service are provided at the 11 am service. More information is available at 435-674-4955 or go online to CalvaryEaster.com
Sunrise Worship will be conducted by Youth Group, and held in the courtyard at 6:30 a.m., followed by a continental breakfast at 7:30 a.m. Other services include, Celebration and Praise Service at 8 a.m. and Traditional Service at 11 a.m.
Sunrise Worship Service will be conducted by the Youth Group, and held in the courtyard at 6:30 a.m., followed by a continental breakfast at 7:30 a.m. Other services include, Celebration and Praise Service at 8 a.m. and Traditional Service at 11 a.m.
Annual Easter Egg Hunt is open to all children ages toddler – 11 years.
About 2,000 plastic eggs are filled and hidden throughout the courtyard with a dedicated area for toddlers.
The Sanctuary will be open noon to 3:00 p.m. for silent prayer and meditation. Services begin at 7:00 p.m.
On this day Christians remember the suffering and death on the cross of Jesus Christ.
Maundy Thursday commemorates the Last Supper when Jesus shared a meal with his disciples on the night before he was crucified. There will be a live re-enactment of Last Supper.


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Part Four of Mario & The Magic Railroad is here.
[Cuts to Gordon with the express at Vicarstown Station.]
Narrator Thomas: Next morning, Gremlin traveled with Gordon to the big city station. Gremlin was sure that I could help me and together we could help me. But only if Rosie met someone at Knapford first.
[Cuts of Odie and Yuki walking around]
Conductor: [voice over] Attention, passengers the train for Maron Station will depart from track 3! Train for Knapford will depart from track 4, last stop! Knapford, track 4!
Gremlin: [barks to get Rosie's attention]
Rosie: I don't you suppose where track 4 is do you? [Gremlin barks and walks to the express train] You do? Are you sure? [Gremlin barks and Rosie buffers up to the express]
Narrator Thomas: Gremlin was feeling pleased with himself, for I was going on the right train, and he was going to make me stop at the wrong station, for the right reasons.
[Odie runs into Gordon's cab before the train leaves. Gordon starts and blows his whistle]
I know how the moon must feel Looking down from the heavens Smiling at the silly things things We put ourselves through Missing magic each day And not seeing the wonder That's how the moon must feel
I know how the moon must feel When he makes someone happy That's a feeling I would feel When you smile at me I'll be floating on air I'll be beaming with wonder That's how the moon must feel
[Cuts to Barney scratching Wario's back with a back scratcher.]
Wario: Left a bit, right a bit, up a bit! Ahhhhhh....
Barney: Is the itch all gone, Strong Bad?
Wario: Well, almost. Hey, why are you so tired? Is because of all the red I'm wearing? Bowser thinks yellow's a stupid color, but it's nice against the snow! It's not me, is it?
Barney: Oh, no, I think red is a good, cheerful color!
Wario: Oh, thanks!
Thomas: Just like my sparkle. Oh, I'm going to the windmill, to look for something important. You should probably check your Monday emails.
Wario: Okay. [leaves] Keep your sparkle up!
[Mario is walking by, and sees Boshi smashing a pile of rocks.]
Mario: What is he up to? And why is he here?
Michael/Train48: How bout a race, Mario?
Mario: Sorry, Michael, I can't today! I have to solve mysteries instead!
Michael/Train48: That means I win! Perhaps another day!
[Thomas then finds himself in the forest]
Thomas: What happened? Where is the windmill? Now I'm lost!
[He blows his whistle, but no gold dust. I sit down on a rock in dismay.]
Thomas: [sigh] Now my gold dust really is all gone. And if I can't find the source for making more, the consequences will be bad! I saw them in my dream last night. My family never told me what to do in a gold dust crisis. They only said, "If you can't remember the clue the windmill will remind you." But were is the windmill, and what is the clue?
[Cuts to Mario, Toadsworth, Diddy Kong, Donkey Kong, Wario, Toad, Luigi, Yellow Toad and Blue Toad at the Brick Wall. Michael/Train48 passes by.]
Michael/Train48: Smile, everyone! It's a sunny day!
Wario: It's not sunny, 'cause Zack is not at the windmill. I looked.
Mario: I think his sparkle's all gone.
Diddy Kong: I don't feel sunny. I feel storfed orp.
Donkey Kong: That sneezing powder and nasty fumes from Deep Claw, I bet.
Toadsworth: And Bowser, Diesel 10 and Smelter are after the lost engine.
Toad: And if they find them, I fear that will destroy us all.
Donkey Kong: WHAT, EVEN ME?!!?
Toad: Yes, Donkey Kong, even you.
Luigi: Those bad guys have Stinkoman doing their bidding! I saw him yesterday, trying to find a certain pile of rocks!
Blue Toad: It doesn't surprise me! Stinkoman's nothing but a show off!
Yellow Toad: Why would he go destwoying a piwe of wocks?
Blue Toad: Why don't you be quiet, Yellow Toad?
Wario: Shut up, both of you!
Blue Toad: We have a serious problem on our... hands?
Mario: Ahhh... [sneezes]
Wario: Say it, don't spray it, Mario.
Mario: I've still got sneezing powder up my non-existent nose! [leaves] Now I'm going to look for Thomas.
Blue Toad: Wet us get back to wowk. That's what he would want.
[Mario is walking along the Mushroom Kingdom. Meanwhile, Thomas hears rustling.]
Thomas: What on earth?
[He notices a letter. He reads it.]
Thomas: [reading] "I notice you left your thinking dome behind. Try these instead they're good for the brain. Gone fishing, Rabbit." I don't see a rabbit anywhere. [I notice a carrot and celery] How thoughtful.
[He picks up the carrot, takes a bite, and thinks.]
Thomas: Plane... drain... [another chomp] mountain... fountain... Mountain's something. I'll try the celery.
[He picks up the celery, bites into it, and thinks]
Thomas: Sausage... bicycle... *HIC! music... cornfield... *HIC! Wait a minute. Cornfield. CORNFIELD! That's it! Ha ha! I have to find a bell flower, make a call! *HIC!
[Meanwhile, in a town of Indiana, James is picking up corn in a basket. Back to The Mushroom Kingdom, He finds the bell flower, and in Indian, James hears his phone; he was listening to "Summer Sunday".]
James: Oh! My cell phone! [answers] Hello?
Thomas: James, is that you?
James: Hey, Thomas! Are you in a tunnel? It's not a good line!
Thomas: James, where are you?
James: The nearest cornfield! I just had breakfast and here I am!
Thomas: James, listen: You have to come to the Mushroom Kingdom right now!
James: Right now? I'm helping with the corn!
Thomas: No you're not! You're coming here! You have to help me find the source of our family's gold dust.
James: What's the source?
Thomas: That's the problem, James; I haven't a clue!
James: I used up most of mine too.
Thomas: WHAT THE?! [sighs frustrated] Then go to the Island of Sodor first and in my signal house, in the box under the staircase you'll find my emergency whistle with the last of my supply. Please take care of this and... [He looks around for anyone else] don't mention the buffers.
James: What buffers?
Thomas: SHHHH!!!
James: This is a bad line! HELLO! MY CELL PHONE ISN'T WORKING RIGHT!!!
Thomas: James, you've got to come here right away! I'm counting on you!
James: HELLO?!!?
Thomas: What-?! [He's hung up] What the?
James: [sighs] I hate my job... [throws cell phone away]
[Later James is relaxing outside his shed]
James: [sighs] One last sun ray, and one last sip, of a summer... sundae! [is about to take a sip from a milkshake bigger than him] I think I'm a poet...
[some of the melted milkshake splatters all over him]
James: Oh, geez... I'm covered in melted milkshake.
[Meanwhile on Sodor, Gordon is approaching Knapford station. Behind the last coach, Rosie notices Diesel 10 in a siding]
Diesel 10: Yes, it will be final later this week. Yes ,soon I'll be the new controller of Sodor. Bye.
[Gordon's train stops at Knapford Station. Rosie notices Gremlin on the platform.]
Rosie: You want me to get off here?
Gremlin: [nods]
[Rosie is now at Tidmouth Sheds. She is alone with Gremlin and Diesel 10.]
Rosie: Where's everybody, and why have you brought me here for?
[She hears a noise inside the sheds]
James: [not seen] It not where he said it would be... Maybe it's in the closet... Hey, my old bicycle, I wondered where it went... Oh, how cute! Six little- [crash] five little glass figurines! [Yuki knocks on the door of the signal house] Coming!
[James comes out in a cloud of gold dust]
James: Hello. Who are you?
Rosie: I'm Rosie. Who are you?
James: James the Red Engine! Did I drop a ticket puncher when I came in here?
Rosie: I don't know
James: Oh, well, I might use more from this stuff. Goodbye, Rosie.
[James leaves in a cloud of gold dust, Rosie looks at it.]
Rosie: What is this stuff?
[Back at the Mushroom Kingdom, Thomas is sleeping at the Mushroom Kingdom Sheds, whilst dreaming of Sodor as a ghost railway.]
Thomas: The magic's gone. Why did I take my gold dust for granted? I can't help without my gold dust.
[Viaduct starts to collapse]
Thomas: What is the source of my gold dust?
[Sounds of train passes by, Thomas wakes up]
Thomas: Is someone there? [Mario walks by] Mario, is that you?
Mario: Thomas where are you?
Thomas: Mario?
[no reply, Mario is gone]
Thomas: How can I possibly say that I'm really useful now?
[Meanwhile, Boshi destroys a pile of rocks.]
Bowser: Not that pile of rocks, then. You have to destroy all the piles of rocks you find, no matter the consequence! [leaves]
Boshi: I'm beginning to have second doubts. What if that overbite kid is right?
[Back at the quarry, Diesel 10 follows old abandoned railway tracks to look for the lost engine but instead sees it as a dead end.]
Diesel 10: Nothing... [oils away in disappointment and anger]


text: What's up guys? I'm completely new to IM and over the last week or so I've been reading and reading and reading on BHW and I've learnt so much. There's still so much to learn but I thought I'd start a journey topic to document my progress and to give me motivation to carry on. My aim is to reach £10 a day spread across all of my websites, when I reach that I'll be happy and I'll set the bar higher! Currently I have 1 website that's got content on it (more authority than micro niche) but I'm waiting on 3 micro niche sites that I purchased from the forums. As right now my site is ranking 300ish for my main keyword, but in top 30 for some other keywords which I'm happy about. I've bought three articles from the forums that I'm also waiting on, but I'm happy enough to write my own content as well and I'm adding at least 1 article per day. I'll be using a mixture of Amazon affiliate and Google Adsense in order to make my millions (lol) so I'll keep this updated with daily earnings + a total earned figure! So, total earned so far: £0.07 Wish me luck, I'm going to get back to reading the useful threads on BHW now!


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Oprah Winfrey
By: Kailey Hunter
Oprah Winfrey was born on January 29, 1954. Winfrey was born is Kosciusko, Mississippi. Oprah Winfrey is best known for being a talk show host and being an actress. In 1971, Winfrey entered Tennessee State University, and began working in radio and television. Later in 1988, Winfrey founded Chicago - based Harpo productions.This is when her career launched off. In the same year, Winfrey became the third woman in to own and produce her own talk show.
In November 2013, Winfrey received the nations highest Civilian Honor. Barack Obama gave her the Presidential Medal of freedom in 2013. In 2005, Business Week named her the greatest black philanthrophist of American History. In the same year, Winfrey produced and stared in the movie Beloved. In January 2007, she made an academy for girls. Also in 2007, Oprah Winfrey made her first political endorsement. Since 2012 to the present time Oprah has a her own T.V. show called Oprah: Where Are They Now?


text:
What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Cannot create fillable PDF when using eu.jotform.comAsked by mikeyaberry on January 21, 2016 at 11:54 AM
Sorry, I still can't create a form. Below is a link to a short video of what steps I took. I have tried to create the pdf on mac & pc using both ff & chrome
Hope this helps.
Thanks Mike
JotForm Support
Thank you for bringing this to our attention, I will forward this to our developers to have a look. We will let you know here as soon as we have any further information as to status of your ticket.
JotForm Support
Apologize for the inconvenience . We have just fixed the problem, you should be able to generate your PDF form. Please let me know if you face any problem.
I was able to generate the PDF form.
Thanks to everyone for their help!
Much appreciated!
Thanks Mike
JotForm Support
Glad to hear things are now working once again. If there is anything else we can assist you with, let us know and we will be happy to help.


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Fruit Salad from Heaven
Recipe by Redneck Epicurean
This is my absolute favorite fruit salad. When I take it to church, the bowl is always left clean and nobody knows it's not made with sugar. It's such an easy way to get my fruit fill for the day. This can be used as a diabetic diet fruit exchange; 1 fruit exchange for this is one cup. NOTE: You should use as much fresh fruit as possible. The only thing from a can is pineapple and peaches (and cherries - fresh cherries don't work for this). This salad also should not be prepared until right before serving if at all possible. Refrigerate all the fruit except the bananas. Cooking time is chill time.
Top Review by internetnut
I gave this 5 stars. My family loves fruit salad. I serve this in the summer with dinner. I make mine slightly different. All fresh cut fruit no canned. No cherries, kiwi, splenda or cherry juice. But I think it sound yummy this way. Can't wait for summer to try this your way. Thanks for posting a great recipe! Christine (internetnut)
See Discussion:3 Reviews
Ingredients Nutrition
- 1 (20 ounce) can pineapple tidbits, drained
- 1 (20 ounce) canned peaches, drained and cut into chunks
- 1 small maraschino cherry, cut in half (reserve juice)
- 2 oranges, peeled and cut into chunks
- 1 cup blueberries
- 2 cups strawberries, quartered
- 3 kiwi fruits, peeled and cut into chunks
- 2 mangoes, peeled and diced
- 1 medium gala apples, peeled and cut into chunks
- 2 medium firm bananas, sliced thin
- 2 cups white grapes, halved
- 2 cups black grapes, halved
- 1⁄4-1⁄2 cup Splenda granular
- 2 tablespoons cherry juice
Directions See How It's Made
- Place all the fruit in a large bowl.
- Depending upon how much juice you want in the salad, pour the Splenda on top of the fruit, 1/2 cup for more juice, 1/4 cup for less.
- Add the cherry juice and stir to mix fruit and distribute the Splenda and cherry juice.
- Cover and place in the refrigerator for one hour.
- Right before serving, slice the banana thin and stir into the salad, redistributing the juice.
- This can be served with angel food cake, pound cake, or by itself.


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ABSOLUTE PLEASURE was once called project/yacht name Araminta Iii; White Mary; Pearl Of The Gulf, So Gracious. This 34 metre (111 ft) luxury yacht was built at Camper & Nicholsons in 1975. Motor Yacht ABSOLUTE PLEASURE is a well sized superyacht. The naval architect who actualised her design for this ship is Camper & Nicholsons. Luxury yacht ABSOLUTE PLEASURE is a quality yacht that is able to sleep up to 8 passengers on board and has approximately 6 qualified crew.
Camper & Nicholsons Yacht Design was the naval architect involved in the formal superyacht plans for ABSOLUTE PLEASURE. Also the company Camper & Nicholsons successfully collaborated on this venture. Built by Camper & Nicholsons the vessel was fabricated within the United Kingdom. She was successfully launched in Southampton in 1975 before being transferred to the owner. A reasonable proportion is manifested with a total beam (width) of 6.4 m or 21 ft. With a 2.1m (6.9ft) draught (maximum depth) she is reasonably shallow. The material steel was used in the building of the hull of the motor yacht. Her superstructure above deck is created from aluminium.
ABSOLUTE PLEASURE is propelled by twin screw propellers. The main engine of the yacht generates 535 horse power (or 394 kilowatts). She is fitted with 2 engines. The sum power for the yacht is accordingly 1070 HP or 787 KW. As for stabalisers she uses Vosper.
Having quarters for a maximum of 8 yacht guests overnighting, the ABSOLUTE PLEASURE accommodates everyone comfortably. Under normal conditions she requires around 6 proficient crew to run.
Main Engine(s) is two 535 Horse Power or 394 kW Caterpillar. - Total engine power output 1070 HP /787 KW. - Approximate Cruise Speed is 10 nautical miles per hour. - Her top Speed is around 12 knots. - Range in nautical Miles is 3000 at a speed of 10 knots. - Fuel tanks: 26000 L. - Water: 12500.00. - Gensets: G&m 2 times 60 kilowatts. - Stabaliser Stabality devices: Vosper. - Yacht Beam: 6.4m/21ft. - Waterline Length (LWL): 28.96m/95ft. - Draught Maximum: 2.1m/6.9ft.
ABSOLUTE PLEASURE features a teak deck.
The luxury yacht ABSOLUTE PLEASURE displayed on this page is merely informational and she is not necessarily available for yacht charter or for sale, nor is she represented or marketed in anyway by CharterWorld. This web page and the superyacht information contained herein is not contractual. All yacht specifications and informations are displayed in good faith but CharterWorld does not warrant or assume any legal liability or responsibility for the current accuracy, completeness, validity, or usefulness of any superyacht information and/or images displayed. All boat information is subject to change without prior notice and may not be current.


text:
We recently updated the warranty on a number of servers (two weeks ago), though Spiceworks is still displaying the old expiry date. We have confirmation from Dell, the warranties are in place, so does anyone have any experience of the turnaround time it takes Spiceworks to refresh the service tag information?
This topic was created during version 5.1.
The latest version is 7.5.00101.
7 Replies
Sep 16, 2011 at 10:28 UTC
See this article may be it help you.
Sep 16, 2011 at 4:31 UTC
you might also want to try an manual rescan (from the inventory screen) for one of those devices. I think that will go out and check the warranty details too. Another setting you might want to change is in the advanced options for the network scan, scanner sends all data instead of deltas to true.
Spiceworks should be able to grab what ever info you would see checking the website yourself.
Sep 20, 2011 at 4:37 UTC
OK, the warranty check "finally" updated this morning - over two weeks after securing the warranty with Dell. The Spiceworks service has been on during this period, only just now updated? Again, any advice on speeding things up a little, will be greatly appreciated.
Sep 20, 2011 at 4:44 UTC
for speeding up the SW, this akp article will help you alot.
Sep 20, 2011 at 11:26 UTC
Can you confirm if the details were available through the Dell website before today? As far as I know, Spiceworks connects to the Dell servers and downloads the information that Dell provides. If you have warranty scanning on, then this check should be made regularly.
If the information is correct on the Dell website but not in Spiceworks, one thing to try is a manual rescan from the inventory screen, as long as the service tag is correct. That should include a check of the warranty with Dell. You might also want to set the advance scan setting Scanner sends all data instead of deltas to true.
Sep 21, 2011 at 4:02 UTC
I can confirm Dell warranty details were available before they appeared on Spiceworks. I have gone through the Spiceworks settings, the only one I had not setup for optimum performance was sending deltas of the data, which is now set to send all data.
Sep 21, 2011 at 11:12 UTC
If you think you see the same situation again, try a manual rescan as I thought Spiceworks should grab the latest warranty details with the setting scanner sends all data set to true.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


text:
Eco Toddler Clock - Design Your Own Wall Clock
Make learning the time a work of art!
Product Description
Struggling to help your little one to tell the time? Make learning the numbers on the clock a fun game with this black design-your-own wall clock. It comes with colourful chalk so that your little one (and big kids, too!) can create their masterpieces on the surface of the square clock.
And guess what this sleek, eco-friendly clock is made from? Paper! (180 sheets of recycled paper, to be precise!) Although the Eco Toddler Clock looks and feels as though it's made from sturdy slate, it's actually made from recycled paper. Don't worry though, the paper has been treated so that it's weatherproof and robust.
The black, square clock features silver-coloured metal hands and gold-coloured bolts, and is designed to be wall-mounted with the in-built hook on the back.
Comes with 4 sticks of multicoloured chalk.
Made in the UK from collected and recycled material.
Material: Weatherproof, bonded recycled paper
Dimensions: H20 x W20cm
Please note: The Eco Toddler Clock requires 1 x AA battery, which is not supplied.
Customer Reviews
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text:
4 CVs for "Writer, Typist" in United Kingdom.
Copy/Audio Typist/Article Writerworldwide Computer skills very good. Certificates available. Copy/Audio Typing. Have previously been a conveyancing secretary. Very knowledgeable about Health Care cleaning. Can provide a good article. Have provided articles for clients two private and several for Videjug. [more]
writer, typistworldwide I was the editor-in-chief of a high school newspaper. I have been writing short stories and developing my grammar and expanding my vocabulary. Though I have only been writing for my own amusement, I hope to find my audience and improve in anyway I can [more]
Proofreader, typist, data entry, writerworldwide I have good typing skills and have a good knowledge of working the computer and the internet. I have been working from home as an administration assistant for the last year. [more]
home based Typist/ Writer / Proof ReaderLondon, Greater London A diploma in secretarial skills aswell as grade A English O level - Scheidegger writing course - Since last job in Marketing I have successfully started up my own catering business and am now in need for a second income stream. [more]
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As Niveshak, the monthly finance magazine of IIM Shillong enters the sixth year of its wonderful journey, Team Niveshak is coming out with its 5th Annual Special Anniversary issue. For this purpose, we invite critical views on the theme – "Scripting the Great Indian Revival" from B-schools & Corporates across India. The top three articles would be awarded with Cash Prizes worth Rs. 12,000. Besides, all the articles that get published stand to win attractive Niveshak goodies and certificates!
You can either choose to write on a topic of your choice (but within the ambit of the aforementioned theme) or choose one from the following list. Please note that the list is only suggestive and, by no means, and exhaustive one.
Put on your thinking hats, delve deep into your financial and business acumen to ideate, critically analyze and provide insightful recommendations to the most widely discussed topics.
Please find below the guidelines for this call for articles.
https://sites.google.com/site/niveshakiims3/home/Niveshak_Anniversa ry2013.pdf
For further information and updates, stay invested on the Facebook page and website of Niveshak.
EventList powered by schlu.net


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With a few notable exceptions, most Canadian MPs did not grow up in political households, come from money or attend "the right" schools. A new study, based on interviews with 65 former MPs who served from 2004 to 2008, found that many parliamentarians fell into politics by accident, and self-identify as "outsiders."
This has both positive and negative consequences for Canadian democracy. While it is reassuring that these political outsiders feel the process is open to all, and that they are able to succeed, no matter their political pedigree, it raises troubling questions about the experience and ability of MPs to accomplish the business of governing.
Parliament is, of course, best served when it is broadly representative of the population and can draw together people from a host of backgrounds, concludes the report, published earlier this month by Samara, a Toronto non-profit organization that studies citizen engagement in democracy.
Samara found that few parliamentarians set out to be career politicians. The exception to the rule is someone such as MP Peter Milliken, the longest-serving Speaker in Canadian history, who dreamt of becoming an MP from a young age, and who recently announced his retirement.
Mr. Milliken subscribed to Hansard, the transcript of parliamentary debates, as an adolescent, and studied parliamentary procedure, even completing a university thesis on question period.
However, most parliamentarians spend years involved in their community, and pursuing other lines of work, before being recruited into politics. Their average age is 47, according to the study, and their backgrounds include have been a cook, journalist, teacher, consultant, trustee and priest.
The report may reflect a telling aspect of the Canadian character: a preference to present as an underdog, and to mask political ambition. Politicians no longer have an honourable reputation, and there is no advantage to declaring oneself to be a political insider.
However, this lack of a political class, or "farm team," isn't wholly welcome news. A lack of exposure to politics may leave MPs unprepared when they go to Ottawa. The role of an MP is to represent his or her constituents, sit on committees and fine-tune legislation, and scrutinize the government. MPs must appreciate the institution of Parliament in order to work within it. Ensuring the government is accountable doesn't mean shouting insults during Question Period. In the words of political scientist David Docherty, "If politicians hold politics in contempt, then how do you expect Canadians to feel?"
The study is the first time anyone has explored MPs' motivations in a systematic way, and should be used as a basis to further understand how the pathways to power really work. For all the welcome emphasis on outsiders, insiders such as Mr. Milliken, who understand the nature of the institution and its idiosyncrasies, are just as vital to Canadian democracy.Report Typo/Error
Follow us on Twitter: @GlobeDebate


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
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Custom URLAsked by Bdmedearis on December 04, 2015 at 02:29 PM
Is there a way to create a temporary custom URL?
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login with
vitamin d drops
when is they best time to give them?
Our pediatrician gave them to us at 2 month checkup.
mine told me 2 weeks...?
This is new to me, I don't remember doing this with my kids.
yes im breastfeeding, she said that since i was breastfeeding then he needs them.
We gave our daughter vitamin d drops after her 2 week appointment and still continue with them (it's part of our bedtime routine and she takes it so well and gets excited when she sees the dropper). Our doctor said to continue them until she is about a year old. The reason she gave was since it was fall, going into winter, she would not be getting enough vitamin d from the breastmilk and would not get sun.
About Susan
Birth: August 28
On Moms.com since: Mar 23, 2014
im a first time mommy and he has had my heart since i heard him cry. i just went back to work a couple of weeks ago. its hard being away from his so long as i work full time. but i know im doing the best thing i can for him.
All Recent Susan's Posts


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Ost Drama Full HouseThai Mike D'Angelo and Aom : Oh Baby ( Arabic sub ) [HD1080p] MP3
[email protected] // [email protected] // Twitter : @narimenboudaya /// Weibo : @Narimen_Tunisia // FB : KimkyuJoongi ChenXiang.
Mike D. Angelo's moment with a baby MP3
Mike's moment with a baby in Namasate premiere press conference while waiting to watch the movie at Esplanade.
Baby don't cut - B-mike MP3
iTunes: http://itunes.apple.com/album/id923419519 Facebook - http://www.facebook.com/bmikemusic Instagram: http://www.instagram.com/okayumm Twitter ...
Fabolous - Baby (Ft. Mike Shorey) MP3
Music video by Fabolous performing Baby Featuring Mike Shorey With John Jackson & Mike Shorey (C) 2004 Street Family Records.
Oh Baby I - Mike D.Angelo ft. Aom [Lyrics] OST.Fullhouse วุ่นนักรักเต็มบ้าน MP3
เป็นซับชิ้นแรกที่ฝึกทำ ยากมากแต่ทำแล้วสนุก มือใหม่หัดทำเลยใช่เวลาไป...
Mike D. Angelo (Mike-Pirat) & Aom Sushar: Oh Baby I MP3
AoMike at Full House The Special Live Party [2/3] @ ONYX on Apr 25, 2014.
B-Mike - Baby Don't Cut (Lyrics) MP3
I love this song.. and if you're going through this, you can message me, and talk to me.. I'm here for you . /.\ ** I don't own the music or song ** Like, Comment, ...
Mike Posner - Please Don't Go MP3
Mike Posner's official music video for 'Please Don't Go'. Click to listen to Mike Posner on Spotify: http://smarturl.it/MikePSpotify?IQid=MikePPDG As featured on ...
Please Don't Go
Mike PosnerLet's run away from these lies Back to yesterday, safe tonight I feel the sun creeping up like tick tock I'm trying to keep you in my head but if not We'll just keep running from tomorrow with our lips locked Yeah you got me beggi[...]
Baby don't cut (Self Harm Song) - B-Mike (traducida al español) MP3
J-Ro, Styliztik Jones, Roccaspotz, Baby Mike, D.T & Sindri - Almost Over [Official Video] MP3
This song was recorded at J-ro's house on the 9th of june and the video was shot the very next day. Download Link For The Song: ...
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"Mike & The Mechanics" are an English rock/pop band formed in 1985 as a side project of Mike Rutherford, a founding member of Genesis, that became a ...
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To : Pi Babier by Picaho.
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On behalf of Green Country Technology Center in Okmulgee Oklahoma this video was made by an excellent student by the name of Sarah Rose Jones.
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1988.Asia Records.
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East of Eden considers Jonathan Edwards's sermon, "Blessed Struggle," which was delivered in 1735. Participants: David Filson, Jeff Waddington,... Read More
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Christ the Center is pleased to welcome Dr. Dane Ortlund to speak about Jonathan Edwards's views of the Christian life. Ortlund is Senior Vice President of Bible Publishing at Crossway and the author of the book Edwards on the Christian Life: Alive to the Beauty of God. ... Read More
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In this episode, Nick, Jeff and Dave sit down to discuss Jonathan Edwards's sermon, "Joseph's Great Temptation and Glorious Deliverance" based on Genesis 39:12.... Read More
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In "The Peace Which Christ Gives His True Followers," Edwards calls us to listen in on the most affectionate and affecting discourse Christ gave (Jn 14:27),... Read More
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David Owen Filson speaks about Dr. J. Oliver Buswell, theologian and former president of Wheaton College and Covenant College and Seminary.... Read More


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Joe Belfiore gives us a full demo of the announcements being made at MWC, including: new user experience; Integration with social networks like Facebook; Internet Explorer; Photos, videos, and music; E-mail and texting; GPS and maps; Search integration; Hardware; standards .................Watch the video:
Ttoday's phones provide a palm-sized mobile office, entertainment center and communications hub. Along with the striking evolution of mobile technology, Andy Lees of Microsoft's Mobile Communications Business says the most important part of the market for mobile phones is the people who use them. "There's a unique relationship between people and their phones," Lees says.


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This item is not stocked and usually takes 5-14 working days for dispatch.
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VacciCheck Antibody Test Kit is a simple and affordable in-clinic titer test designed to monitor serological status and vaccination failure to prevent overvaccination consequences. VacciCheck provides reliable and accurate results in just 21 minutes.
Serology testing is useful after a dog vaccination to indicate whether immunization success has been achieved. In this way the veterinarian can modify vaccine programs for individual puppies. The cut-off values of the VacciCheck kits correlate with the vaccination recommendations of the Cornell University School of Veterinary Medicine.
The comb has 12 teeth. Each tooth tests one blood or serum sample and can provide up to three tests: ICH, CPV and CDV
The developing plate has multiple compartments, which contain the necessary reagents for comb developments.
1. Obtain blood or serum from animal(s). Just 5 µL serum or 10 µL blood.
2. Deposit blood or serum sample(s) into well(s) A in developing plate.
3. Insert Comb into well(s) A. Incubate for specified time.
4. Transfer the Comb from well(s) A to well(s) F in a timed sequence. Time intervals vary according to type of kit.
5. Let Comb dry and read results. The color intensity of spots on Comb corresponds to antibody level.
All prices include VAT where applicable.
Below are some recent questions we've received regarding VacciCheck Canine Antibody Test Kit, including answers from our team.Ask Your Own Question
Is this product for vet use only or can I do this test at home.
This product is designed for in practice use by a vet as it requires blood to be taken from the animal for the test.
Sorry that this product is out of stock, instead of having to keep checking back to see if we have it, we can send you a notification as soon as it comes back in stock.
We won't use any details you enter for any other purpose than letting you know when this is back in stock.
Please enter details for each method you'd like us to notify you by:
VioPoints are a way for us to say thank you for purchasing from VioVet.
You will receive VioPoints by purchasing this product, which can be redeemed for a discount on future purchases. For each 1000 points redeemed at the checkout, you will receive a 20p discount.
The number of VioPoints is shown on the product listing and they are awarded on dispatch of the order.
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Listen to free music by Bell William And Mavis Staples
Blip.fm is internet radio made social. It's easy to search for, play, and discover free music recommended by real people. Join today to create your own free station.
how_long_it_takes okay, gotta get to what I logged on for. off to listen to all this stuff (reblip)
how_long_it_takes I could go for this one again. still on schadenfreude overload
how_long_it_takes already did this the other day but it needs to be on heavy rotation. majestic
WildWildSound The chorus of this one is just a bit too far out, but fer somehow it fuckin' stings...dig a shlocky duet by William Bell & Mavis Staples...no offense! (reblip)
crowjane @how_long_it_takes informs: crowjane. now if we're talkN duets, this is a*definitive* duet~Hell 2 da' Nizzle Hizzle~hip me my bro~never heard it~ thnx (reblip)
how_long_it_takes @crowjane. now if we're talking duets, this is a *definitive* duet
Artists Like Bell William And Mavis Staples
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Easy Pineapple Pie
READY IN: 55mins
Top Review by HatesTooCook
This was yummy and so easy to do. Only thing is I wish I'd of browned the pie shell first but since it said to use an unbaked pie shell I did. Didn't like how the bottom of the pie shell turned out but other than that, it was really good.
Ingredients Nutrition
- 8 1⁄2 ounces pineapple, crushed
- 1⁄3 cup margarine, 3/4 stick
- 1 1⁄2 cups sugar
- 3 large eggs
- 1 teaspoon vanilla extract
- 1 9" unbaked pie shell
Directions See How It's Made
- * Substitute 1/2 cup packed brown sugar for 1/2 cup of the white sugar if preferred.
- Drain pineapple very well, reserving juice for other uses.
- Cream butter and sugar together until light and fluffy.
- Add eggs and use a wire whisk to combine thoroughly.
- Add vanilla extract and drained pineapple; blend.
- Pour into unbaked 9-inch pie shell and bake in preheated 350-deg ree oven for about 45 minutes.
- Let cool for a few minutes before cutting, or serve cold.


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About the Book
Chyli Anders is finishing high school and planning for college when she receives a mysterious note in her locker. She then tumbles headlong into a mystery that is somehow tied to her senior Chemistry project. Will the handsome writer of the note keep her safe while they ferret out the mystery and rescue the kidnapped victims?
Features & Details
- Category Mystery & Crime
6×9 in, 15×23 cm
262 Pages
- Publish Date Nov 09, 2013
- Language English
- Tags High School, Young Adult, Horses, Adventure, Mystery, Fiction, Teen
About the Creator
Shari Lynn Brown resides in southern California where she lives with her parents and twin sister. Her first novel, The Mystery of Medicine, became available in December 2011. Shari works full-time as an administrative professional. She enjoys training her horse in Western pleasure, co-leading a high school small group at her home church, as well as singing in her church choir. Swimming, scuba diving with her dad and sister, and playing her trumpet and piano are favorite pursuits. She loves to visit her older brother and his family at their home in Idaho. She is active in her church group and greatly loves her Lord Jesus Christ. Cloudy days will find her with a cup of tea and piece of chocolate, either reading mystery and adventure books, working on a new creation on her laptop computer, or updating her blog, www.mysteryofm.blogspot.com. Day or night she always has a notebook at hand because "you never know when an idea for your next book might come to you
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Semiconductor quantum dots (QDs) are tiny light-emitting particles that have emerged as a new class of fluorescent labels for biology and medicine. Compared with traditional fluorescent probes, QDs have unique optical and electronic properties such as size-tuneable light emission, narrow and symmetric emission spectra, and broad absorption spectra that enable the simultaneous excitation of multiple fluorescence colours.
These properties arise from their semiconducting nature, which exhibits quantum size effects when charge carriers (electrons and holes) are confined in dimensions smaller than a critical size threshold defined by the Bohr diameter1. Semiconductor nanocrystals that are smaller than the Bohr diameter emit fluorescent light that can be tuned by the particle size across a broad spectrum of UV, visible, near-IR and even mid-IR wavelengths. The semiconducting crystalline nature of these particles also gives rise to large absorption cross-sections, which causes QDs to be 10–100 times brighter than organic dyes and fluorescent proteins (see Figure 1). In addition, these particles are dramatically more resistant to photo-degradation compared with traditional probes. However, QDs are macromolecules that are an order of magnitude larger than organic dyes, which can limit their use in cellular imaging applications that require small labels. In this article, we briefly discuss recent developments in size-minimized QDs, new tagging strategies, as well as methods for QD intracellular delivery and dynamic cellular imaging.
The large size of current QD probes arises mainly from a thick polymer coating on the particle surface. The nanocrystalline cores of QDs are initially prepared in non-polar organic solvents and coated with non-polar aliphatic ligands. In order to prepare water-soluble colloids, they are coated with an amphiphilic polymer shell (see Figure 2). These formulations are exceptionally stable and biocompatible and have been instrumental in enabling the commercialization of QD bioprobes, but the micelle-like multilayer coating greatly increases the total hydrodynamic size. For example, QDs with a 5 nm crystalline core will balloon in overall size to 15–30 nm after coating. In comparison, most soluble globular proteins are 4–8 nm in diameter.
The first biocompatible QDs were stabilized in water with small thiolate ligands such as mercaptoacetic acid2. These particles were exceptionally compact in size, but the co-ordination bond between the ligand and the QD surface is weak, causing rapid degradation of these particles in complex biological conditions. This problem has recently been overcome by re-engineering these organic ligands to increase their binding affinity to the QD surface through multidentate interactions3–5. As shown in Figure 2, low-molecular-mass hydrophilic polymers can be anchored to the nanocrystal surface through multiple thiol and/or amine moieties to generate highly stable nanoparticles. This success has dramatically reduced the sizes of biocompatible QDs to close to that of globular proteins, opening new opportunities for intracellular imaging and tracking studies.
In order to use QDs as fluorescence labels to image and track biomolecules, the chemical linkage between the QD and a biomolecule is critically important. The conventional approach for fluorescent biotagging is to conjugate the fluorophore to an antibody that has a specific affinity for a protein or biomolecule of interest (see Figure 3a). However, the QD has a large surface area available for coupling, which results in multiple antibodies binding to each particle. This results in a bulky structure that can form cross-links between multiple target molecules, prohibiting the imaging of single biomolecules. In addition, most covalent coupling schemes result in random orientations of the antibody on the QD surface, which renders many of the antibodies inactive owing to steric hindrance from the particle surface. The ideal tagging strategy would be to attach a QD to the target biomolecule with precisely controlled orientation through a high-affinity bond with a minimal linker length, at 1:1 or controllable stoichiometric ratios. One promising approach is based on the use of polyhistidine peptides (His-tags), which are frequently appended to the termini of recombinant proteins in order to provide an efficient purification route using metal-affinity chromatography6. His-tags bind with high affinity and specificity to bivalent metal atoms such as Ni2+ or Zn2+ loaded on columns. Because QDs also have metal-rich surfaces, proteins with a His-tag domain efficiently assemble on the QD surface with a well-defined orientation (Figure 3b). New thin-shell QD coating strategies are readily adaptable to this conjugation scheme owing to the accessibility of the nanocrystal surface, and a variety of purification methods can be used to isolate discrete QD–protein conjugates to obtain monovalent tagged proteins7. Although more systematic studies are needed, preliminary work from our own laboratory indicates that the His-tag–QD attachment can withstand complex biological environments for prolonged periods of time without significant dissociation.
Other types of recombinant proteins may afford a more stable association between a QD and its protein target. One of these approaches exploits the highly specific binding between the bacterial streptavidin protein and the small molecule vitamin biotin. The streptavidin–biotin interaction is the strongest non-covalent association known, and is frequently employed in a variety of molecular biology applications. Ting and co-workers have developed proteins that are fused to peptides that can be biotinylated under physiological conditions8. Thereby, QDs conjugated to streptavidin can bind with high affinity and specificity to these engineered proteins (Figure 3c). Streptavidin is natively a tetrameric protein with four biotin-binding pockets, but protein engineering has enabled the isolation of a monovalent streptavidin variant, and monovalent streptavidin conjugates of QDs have been prepared with precisely one biotin-binding site7. Another strategy is the use of HaloTag proteins, which are haloalkane dehalogenase bacterial proteins that have been mutated to readily form a covalent bond with chloroalkanes9. Because chloroalkanes are very rare functional groups in biology, one can label a HaloTag fusion protein with QDs that display chloroalkane groups (Figure 3d).
The cellular plasma membrane is a complex fluidic surface rich in microdomains that serves as a substrate for receptor-mediated signalling events. Dynamic diffusion and trafficking of membrane receptors has been found to play a pivotal role in many cellular processes, but the detailed dynamic events remains poorly understood. This is because researchers have traditionally studied these receptors through bulk-averaged fluorescence from many dyes and fluorescent proteins that are bound to receptors. Single-molecule imaging of dyes bound to receptors has revealed that these processes and behaviours are heterogeneous, changing over time and space and from one receptor molecule to another, but these studies have been limited to fairly short durations (<5–10 s) because of dye photobleaching problems. Previous studies using QD–ligand or QD–antibody conjugates revealed the complex workings of single membrane receptors at high sensitivity and temporal resolution10,11. New receptor behaviours have been reported such as motor-driven transport of the epidermal growth factor receptor along cellular outgrowths toward the cell body9.
Neurobiology is poised to greatly benefit from receptor tracking experiments with QDs. This is because neurons have richly complex plasma membranes with multiple types of microdomains that form intracellular signalling complexes called synapses which are known to exhibit dynamic receptor exchanges. The diffusion of several types of neurotransmitter receptors into and out of the synapse has recently been studied using QDs attached to glycine neurotransmitter and α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) glutamate receptors, revealing rapid fluctuations in diffusion rates in different membrane regions10,12 (Figure 4). Some receptors, such as the neuronal growth factor (NGF) receptor, become internalized into the cell once they bind to a specific ligand, a process that can now be studied in great detail due to the photostability of QDs13. QDs conjugated to NGF were found to bind to the NGF receptor in the terminals of neuronal axons (long neurite outgrowths involved in signal transduction), which induced endocytosis of the receptor–ligand pair within vesicular structures. QD imaging revealed that these vesicles usually contain only a single NGF receptor, and are shuttled great lengths to the cell body along multiple molecular tracks that behave like a multilane highway within the axons.
These initial studies were carried out by using conventional QDs with large sizes and multivalent ligand presentation. Researchers have found that the large size of conventional QD probes is a major problem for their applications in crowded cell-surface domains, such as the synaptic cleft, an intracellular junction between neurons that is typically only 20 nm wide. Larger QD conjugates have limited access to this region compared with smaller antibody–fluorophore conjugates, which adds a certain degree of uncertainty to the some of the neuronal diffusion studies undertaken to date using QDs3,14.
One of the most promising applications of QDs is for the study of protein–protein interactions and dynamics inside living cells. However, a major challenge is to develop innovative methods for loading or delivering freely diffusing and monodispersed QD probes into the cytoplasm or other organelles of living cells. So far, the most effective way to deliver any type of extracellular cargo is to inject the substance directly using a microneedle. However, this process is rather low-throughput since individual cells must be injected one at a time15. In order to achieve higher-throughput delivery of QDs to cell populations, other efforts have been made to temporarily permeabilize the cellular plasma membrane through the formation of microscopic pores, either through the use of bacterial toxins (e.g. streptolysin O) that form well-defined membrane pores or through brief exposure to a pulsed electric field (Figure 5). These mechanisms are promising, but have yet to demonstrate homogeneous delivery of free QDs in cells. An alternative and highly promising approach is the controlled disruption of endosomal vesicles. Cells naturally engulf their surrounding environment through a variety of processes that yield intracellular vesicles containing extracellular fluid. This is a convenient mechanism to enable entry of QD probes into cells, but the particles remain trapped and are therefore not free to interact with their target molecules, so it is necessary to have a strategy for QD release or endosomal escape. One method is to use osmosis for swelling and bursting the endosomes16. This can be performed by allowing cells to engulf QDs during a brief exposure to a hypertonic medium (prepared by adding sucrose or other solutes), which leads to the rapid formation of pinocytic vesicles that bud off of the plasma membrane due to water moving out of the cells (efflux). In a second step, a brief and well-controlled exposure of these cells to a hypotonic solution containing a low solute concentration will cause water to rush into the solute-rich vesicles, inducing osmotic lysis and allowing the QDs to be dispersed into the cytoplasm. Another mechanism is through chemical transfection17,18. A variety of polymers and cationic lipids have been found to efficiently disrupt endosomes or cause vesicular leakage following endocytosis, allowing efficient delivery of macromolecules such as DNA and proteins. Although the mechanism controlling the release of cargo from the endosomes is not well understood, it is likely that the transfection agent also induces osmotic swelling of the intracellular organelles.
QDs have been widely used for visualizing the dynamic motion of motor proteins. Inside cells, proteins such as myosin, kinesin and dynein transport cellular cargos to specific subcellular locations by moving along filament structures through a process powered by ATP hydrolysis. Motor protein translocation has been observed to proceed in discrete steps and with a velocity indicative of specific motor protein–filament pairs. QDs have become important tools for the study of isolated motor protein function outside cells using light microscopy, and they have recently been used to study these same phenomena in living cells. QD–kinesin and QD–myosin conjugates delivered to the cellular cytoplasm through osmotic pinosome lysis were found to undergo directed motion inside cells that was remarkably similar to that observed in purified filaments16,19. Single-molecule imaging of these conjugates revealed an exceptional level of spatial resolution, and the motors could be tracked for extended periods of time without loss of signal. However, these intracellular experiments have been hindered by the large size and multivalent nature of conventional QD probes. This is primarily because the cellular cytoplasm is a crowded solution of macromolecules amid a network of filamentous structures that acts as a molecular sieve. Conventional QDs are larger than the sieving threshold for most cell types and exhibit dramatically reduced diffusion. This has not been a major problem for studying plasma membrane proteins because the viscosity of the plasma membrane is the limiting factor in diffusion, and cytoplasmic motor proteins demonstrate directed motion, not free diffusion.
Figure 6 shows an experiment in which we loaded cells with three sizes of nanoparticles, each with a distinguishable fluorescence colour, and we monitored their diffusion in different parts of the cells. Only the smallest QDs exhibited diffusion similar to globular proteins and native cellular components. QDs larger than 20 nm showed dramatically reduced mobility. A second major limitation of current QDs for intracellular imaging is the rapid fluorescence blinking of single QDs. That is, the fluorescence intensity of an isolated QD fluctuates between 'on' and 'off' states. This effect is averaged out in clusters of multiple QDs and in large ensembles, and is a useful means to identify isolated single molecules, but can disrupt the time-dependent observation of dynamic cellular events. This is not greatly deleterious for plasma membrane proteins, where the mobility is significantly reduced and diffusion is confined to the focal plane of the microscope, but this will significantly hinder QD tracking in three dimensions in the cytoplasm, where the loss of QD signal could either result from blinking or from diffusion of the QD out of the focal plane. Future success in QD tracking in intracellular locations will require QDs with reduced blinking; efforts to engineer QDs to eliminate blinking are currently underway20.
Single-molecule studies have revealed that the microscopic world of biology is considerably more heterogeneous and diverse than we could ever have imagined. The ability to image and tracking single macromolecules is thus essential for a mechanistic understanding of cellular biology. Recently researchers have shown that the diffusion of more than 1000 QDs conjugated to plasma membrane receptors can be simultaneously imaged and charted on the cellular plasma membrane to generate a diffusional map of the plasma membrane, which is shown to consist of distinct domains with unique properties that fluctuate over time21. This cellular and molecular mapping approach could be applied to different compartments in cells to reconstruct the entire structural and functional framework of individual proteins inside living cells in real time. Projects such as these will be aided by the unique properties of QDs that not only enable simple single-molecule imaging, but also allow the simultaneous detection of a large number of unique spectral colours to code multiple proteins. These approaches could apply not only to individual cells isolated in cell culture conditions, but also to living cells in living organisms. Indeed, single-molecule tracking of plasma membrane receptors has been demonstrated with in vivo microscopic imaging for the study of tumours in animals22. Another exciting direction is the use of multicolour QD probes for super-resolution optical imaging of dynamic cellular events23. Conventional optical microscopy is limited to a spatial resolution of around 200 nm (the diffraction limit), but researchers have recently overcome this limit using several approaches such as stimulated emission depletion (STED) and photoactivated localization microscopy (PALM). These techniques greatly benefit from the capacity to optically switch the fluorescence of a molecule from an 'on' to 'off' state and vice versa. An important step in this direction is to develop 'switch-able' QDs and strain-tuned type-II QDs (with minimal overlap in their fluorescence and absorption spectra). Indeed, the capacity to image the real-time dynamics and functions of single molecules with molecular resolution would open a new window into molecular and cell biology.
This work was supported by the NIH Roadmap Initiative in Nanomedicine through a Nanomedicine Development Center award (PN2EY018244), and was also supported by NIH grants (P20 GM072069, R01 CA108468, U01HL080711 and U54CA119338). A.M.S. acknowledges the Whitaker Foundation for generous fellowship support; M.M.W. acknowledges the NIH for interdisciplinary training in biomedical imaging (T32EB005969); and S.M.N is a Distinguished Scholar of the Georgia Cancer Coalition (GCC).
Andrew M. Smith is a Distinguished Fellow of the NIH Center for Cancer Nanotechnology Excellence at Emory University. He received his BS degree in Chemistry and his PhD in Bioengineering, both from the Georgia Institute of Technology. His research focuses on nanomaterials engineering for molecular imaging of cancer and the exploration of the interactions between nanostructures and biological systems.
Mary Wen is a graduate student in the joint biomedical engineering program of Emory University and Georgia Institute of Technology. She received her BS degree in biomedical engineering at the Johns Hopkins University. Her research interests are in the development of new tagging strategies for size-minimized QDs and their applications in molecular, cellular and in vivo imaging.
Shuming Nie is Wallace H. Coulter Distinguished Chair Professor of Biomedical Engineering at Emory University and Georgia Institute of Technology. He received his BS degree from Nankai University, his MS and PhD degrees from Northwestern University, and did his postdoctoral training at both Georgia Tech and Stanford University. His research interests are primarily in the areas of biomolecular engineering and nanotechnology, with a focus on bioconjugated nanoparticles for molecular imaging, molecular profiling, pharmacogenomics and targeted therapy. ude.yrome@eins
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Doctor insights on:
What Can Be Done For Low Blood Pressure
What can be done to make low blood pressure higher?
Depends: Low blood pressure is generally desirable, but if there is lightheadedness with position change or other symptoms of low blood pressure, need to ensure no underlying problems- low thyroid, or low cortisol, or slow heart rate. Need to ensure good hydration status, consider liberalizing salt intake. Compression stockings can be quite helpful. Some medications may include midodrine or florinef (fludrocortisone). ...Read more
Hypotension (Definition)
Hypotension is defined as "low blood pressure". Blood pressure is measured as two numbers and expressed as systolic blood pressure (sbp) / diastolic blood pressure (dbp). The sbp is the pressure that is generated when the heart squeezes. Dbp occurs when the heart relaxes. 120/70 is considered normal in adults. Less than 100/60 (of either number) can ...Read more
Been having low blood pressure and seeing white dots all the time should I go to a&e?
Low BP?: what are your numbers? Have you fainted? Are you on mediciation? Have you had ambulatory BP monitoring> Get amedicalexam and evaluation. ...Read more
He was in e.R. They couldn't find anything wrong. Slightly elevated r.B.C. And slightly low blood pressure. Could it be sudden drop in blood pressure?
ER: Vertigo not usually do slight drop in blood pressure. May need ENT evaluation. ...Read more
Low blood pressure ?
Can be normal: If it is always low and you feel fine, dont worry. Otherwise it can be caused by dehydration or other things. If it's new see your dr. ...Read more
What is low blood pressure?
90/60: Low blood pressure is generally anything under 90/60. This may be normal for diminutive female but if this blood pressure is new or is associated with symptoms it deserves evaluation. ...Read moreSee 2 more doctor answers
Is this low blood pressure?
"Low" is semantic: "normal" blood pressure is an arbitrary thing--i.e. Lower cutoffs of 100 systolic, 60 diastolic were created b/c above these are where the vast majority of people fall. However, many healthy people, as well as some people with heart or liver disease who are perfectly stable, have BP in the 80-90s range. The main point is if it is a change from your personal baseline, and whether you have symptoms. ...Read moreSee 1 more doctor answer
Is 92/55 a low blood pressure?
THERE ARE INDIGENOUS: People in the remotest areas of brazil with blood pressures averaging 50/30 and pulses in the 30-40's. Bottom line is, it depends on the overall health of the individual. ...Read more
What causes low blood pressure?
Low volume/vasodil: Either low cardiac output due to low or high hr's/low stroke volume or excessive vasodilation. Hormonal problems (like adrenal insuff) could rarely be a cause. Infection/sepsis/anemia or acute blood loss, dehydration, or cardiac pump/rhythm problems could cause. ...Read more
Low blood pressure -what to do?
Blood pressure: Ask your doc if anything needs to be done first. To raise BP we increase salt and fluid intakes and use support hose to increase venous return. ...Read more
How to cure low blood pressure?
Variable: Depends on how low the BP is and what if any are the symptoms. Best to get this answer after an exam from your DR. ...Read more
Blood Pressure (Definition)
Blood pressure is a measurement of the force placed on the blood vessels and is comprised of the "systolic" pressure (the top number on a blood pressure meter) which is the peak pressure when the heart is pumping, and the "diastolic" pressure (the bottom number on a blood pressure meter) which is the pressure during the resting phase ...Read more
Abnormal Blood Pressure (Definition)
Abnormal blood pressure is a symptom in which a person's blood pressure is much higher or much lower than it should be for his age and body size. If either the systolic (upper number) pressure or the diastolic (lower number) pressure is not in the normal range, then the ...Read more
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Birthday Present to Myself?
What am I going to get myself for my birthday this year? This is the first year that I don't already know what I want and I'm just counting down the days to go and get it. I'm having trouble finding that one thing that really revs my engine.
Thought about getting an iPad, but I really have no use for one and I'd probably lose interest with it after a few months. Then it's obsolete in a few years, so poor investment.
I'm always up for a new gun. It's what I usually get myself. But seems like this year I haven't found too many guns that really get me ramped up. I'm trying to think, but I think I've only bought 3 new guns so far this year. I set up at a gun show this weekend and believe it or not, but there wasn't a single gun in the whole place that I just feel in love with. There were several that I wouldn't mind having, but nothing that I just needed to have.
What are some gun options say in the ballpark of $1200 max that just really cool and/or fun to shoot? Rifles and pistols. I'm tired of buying safe queens. I thought about an M1 Carbine just for fun shooting. I reload, so ammo would not be nearly as expensive as buying off the shelf. Thought about a Beretta CX4 carbine too, but I'm not sold on that one either. I'm having writer's block for guns here. Need some help. :o
$1200 can get u a sweet SKS and a nice amout of ammo.
Springfield Armory M1A
Someone else had recommended doing a full upper build in 5.45x39 and using the rest to buy some spam cans of ammo.
Do you have a Hi Power?
Do ya have a nice SAA? 1873 Colt. Or replica.
Or a 1860 Colt revolver or replica.
Got a .45/70? M1 Garand? 1903 Springfield?
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Article source: Mateo Arquitectura
In one corner of this great space, we lay out the main entrance to the tower block.
At the corner of the floor plan there is a triangle. The space is black but full of life, veined with colour.
One side of the triangle marks the border, the point of control. Another, annexed to it, offers an unusual view, slightly raised above the rest.
- Architects: Mateo Arquitectura
- Project: Unification of the entrances to the Banc Sabadell tower block
- Location: Barcelona, Spain
- Photography: Adrià Goula
- Author: Josep Lluís Mateo
- Client: Banc Sabadell Atlántico
- Design date: 2010
- Construction date: 2012-2013
- Surface area: 100 m2
- Structures: BOMA, Agustí Obiol
- Installations: Grupo JG
- Technical architect: Ignacio Herrera
- Software used: Autocad, 3D studio and Photoshop


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The Bissell 3-in-1 is a convertible vacuum that switches from a standard floor vacuum to a hand vacuum for use on stairs, cars and other small places. The vacuum has a 2 amp motor and weighs less than 4 pounds with a 16-foot-long cord. Available in many home retail or department stores as well as on the Internet, the 3-in-1 is generally straightforward to use and should perform well with regular care and maintenance.
Things You'll Need
- Waste container
- Dishwashing liquid
Standard Vacuum
Open the latch at the bottom of the vacuum grip and insert the handle. Push firmly into place and press the latch closed.
Push the floor nozzle into the opening located at the bottom of the dirt container. Plug the power cord into an electrical outlet and turn the power switch to "On."
Push the vacuum back and forth over the area to be cleaned, working in overlapping sections for best results.
Turn the vacuum "Off" when finished and unplug the power cord.
Hold the vacuum vertically and push down on the latch located on top of the unit to release the dirt cup. Pull the dirt container out and remove the filter. Empty out the dirt and tap the filter over a waste container. Replace the container on the Bissell.
Wash the filter periodically with warm water and a few drops of dishwashing liquid. Rinse well and dry completely before placing the filter back in the dirt cup.
Hand Vacuum
Release the latch on the handle and pull it out of the vacuum. Push the latch closed.
Plug the power cord into a power outlet and turn the power "On."
Move the vacuum in a back-and-forth motion over the area to be cleaned.
Turn the power "Off" and unplug the vacuum when finished.
Follow the steps in the above section to clean the dirt container and filter.
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Chapter 846 is titled "Tamago's Security".
Cover Page編輯
Cover page request: "O: 'What's your favorite animal?' S: 'I like rabbits.' 'Please draw a picture of the rabbits M. S.-san love so much.' - Tokyo Metropolis PN. Eiichiro Oda (O: Oda, S: Shimabukuro)
Short Summary編輯
Luffy and Nami are overwhelmed by the Big Mom Pirates' abilities, with Amande killing King Baum. The army discovers Lola's Vivre Card and confiscates it as they take the defeated pirates to Sweet City.
Meanwhile, Sanji reaches an agreement with Big Mom for her to release his crewmates in exchange for him marrying Purin with no resistance.
Inside the Room of Treasure in the Whole Cake Chateau, Sweet Commander Charlotte Smoothie guards the three poneglyphs contained inside, one of which is the Road Poneglyph. Brook and Pedro are hiding in a storehouse nearby, and they decide for Pedro to provide a distraction in order to acquire the Road Poneglyph, which will be a once in a lifetime chance for them.
Long Summary編輯
In the outskirts of Sweet City, amidst the bodies of the Chess Soldiers, Galette holds Nami in place with with a fluid substance. Galette expresses her surprise that Nami was able to summon a large lightning bolt, and Nami said that she could do it because of the thunderclouds made by Big Mom. Meanwhile, Amande pulls out her Meito, Shirauo, on King Baum. The tree homie begs her to spare him, as he did not willingly betray Big Mom, but Amande slowly bisects him vertically. King Baum cries from the pain, and Amande states that she wants his death to have meaning by being as painful as possible. Meanwhile, Mont-d'Or stands on two levitating books, praising Luffy who can crush the army right after he defeated Cracker. As Luffy fights through more chess soldiers, Opera attacks him with a mass of cream, increasing its "sweetness" to burn Luffy's arm.
Luffy activates Gear Third and charges at Opera, but he suddenly finds himself inside another location, where nothing is orderly. Mont-d'Or then appears from a wall, revealing that Luffy is in the "World of Books". Luffy then sees Amande approaching Nami inside a cage, and Nami warns him to dodge. Suddenly, a book closes over Luffy's head, returning him to the real world, and Luffy is punched from both the back and the front by two members of the Big Mom Pirates. Luffy falls to the ground, and the two halves of King Baum fall as well. Amande then approaches Nami as she tears her dress, removing Lola's Vivre Card from her bra. Galette understands that this card was the reason the Straw Hats overpowered the Chess Soldiers and escaped from the Seducing Woods. Amande sees the word "Lola" on the card, and the Big Mom Pirates start worrying that the Straw Hats killed Lola to get the card. Nami tries to explain that Lola is their friend, but the Big Mom Pirates decide to report this to Big Mom, taking the captive Straw Hats with them. Luffy hangs onto the ground as he is pulled away, refusing to leave his spot, but the Big Mom Pirate carrying him stomps on his head.
In the Whole Cake Chateau, Big Mom agrees to a request Sanji made, shocking him. She agrees to let the Straw Hat Pirates leave the island alive as long as Sanji goes through the wedding without a fuss, saying that she is a reasonable woman. Sanji thanks Big Mom, and she reminds him of how much the Straw Hats have gone against her, though she is willing to forgive it as her top priority is overseeing the marriage and completing the alliance with Germa 66.
Inside the the Room of Treasure in the Whole Cake Chateau, Tamago oversees the three poneglyphs they had in possession, and reflects that of the approximate 30 that exist, there are only nine they need that will allow them to reach Raftel and uncover the secrets of the world. Meanwhile, Sweet Commander Charlotte Smoothie squeezes another person, squeezing juice out of them and drinking it. Smoothie asks why increased protection from her is needed, but is willing to do it regardless. Tamago reveals that Pedro has returned for the first time in five years after Pekoms spared his life, and so now they must do all they can to protect their Road Poneglyph from him. Brook's detached soul listens from afar, and returns to the alcohol room nearby where he and Pedro are hiding. Brook reveals to Pedro that a Sweet Commander is guarding the poneglyphs, causing Pedro to grow worried about their rapidly decreasing chances of success. However, Brook replies that there is no way to leave and come back later with the entire crew, as they would most certainly be fought upon their return. Brook asks who Tamago's target was, and Pedro affirms that it is him. Brook then asks Pedro to be the bait in their operation, which is exactly what Pedro was thinking.
Quick References編輯
Chapter Notes編輯
- Nami and Luffy are defeated and captured by Big Mom's army.
- Charlotte Galette is revealed to have an ability that controls a fluid that can be used to bind her adversaries.
- Charlotte Mont-d'Or is revealed to have an ability related to books.
- Charlotte Opera is revealed to have an ability related to producing cream.
- Lola's vivre card is taken away from Nami.
- Amande's face is fully shown for the first time.
- Big Mom accepts Sanji's request to let his friends leave Whole Cake Island.
- Tamago has Big Mom's poneglyphs heavily guarded.
- There are roughly 30 poneglyphs in the world and 9 of them reveal the truths of the world.
- Big Mom possesses one more poneglyph other than her Road Poneglyph and the one delivered by Jinbe.
- Sweet Commander Charlotte Smoothie is revealed.
- She is Big Mom's 14th daughter and her bounty is Template:B932,000,000.
- Pedro and Brook have infiltrated Big Mom's castle.
- Pedro plans to use himself as a decoy to give Brook a chance to carry out their plan.
- Pedro is revealed to have been a pirate captain in the past.