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A parallel genome-wide mRNA and microRNA profiling of the frontal cortex of HIV patients with and without HIV-associated dementia shows the role of axon guidance and downstream pathways in HIV-mediated neurodegeneration
Eukaryote microbial genomics
HIV-associated dementia (HAD) is the most common dementia type in young adults less than 40 years of age. Although the neurotoxins, oxidative/metabolic stress and impaired activity of neurotrophic factors are believed to be underlying reasons for the development of HAD, the genomic basis, which ultimately defines the virus-host interaction and leads to neurologic manifestation of HIV disease is lacking. Therefore, identifying HIV fingerprints on the host gene machinery and its regulation by microRNA holds a great promise and potential for improving our understanding of HAD pathogenesis, its diagnosis and therapy.
A parallel profiling of mRNA and miRNA of the frontal cortex autopsies from HIV positive patients with and without dementia was performed using Illumina Human-6 BeadChip and Affymetrix version 1.0 miRNA array, respectively. The gene ontology and pathway analysis of the two data sets showed high concordance between miRNA and mRNAs, revealing significant interference with the host axon guidance and its downstream signalling pathways in HAD brains. Moreover, the differentially expressed (DE) miRNAs identified in this study, in particular miR-137, 153 and 218, based on which most correlations were built cumulatively targeted neurodegeneration related pathways, implying their future potential in diagnosis, prognosis and possible therapies for HIV-mediated and possibly other neurodegenerative diseases. Furthermore, this relationship between DE miRNAs and DE mRNAs was also reflected in correlation analysis using Bayesian networks by splitting-averaging strategy (SA-BNs), which revealed 195 statistically significant correlated miRNA-mRNA pairs according to Pearson's correlation test (P<0.05).
Our study provides the first evidence on unambiguous support for intrinsic functional relationship between mRNA and miRNA in the context of HIV-mediated neurodegeneration, which shows that neurologic manifestation in HIV patients possibly occurs through the interference with the host axon guidance and its downstream signalling pathways. These data provide an excellent avenue for the development of new generation of diagnostic/prognostic biomarkers and therapeutic intervention strategies for HIV-associated neurodegeneration.
HIV-associated dementia (HAD) is the most common dementia type in young adults less than 40 years of age. To date, the cumulative incidence of HAD is 25-38%, and the prevalence is around 37%
It is well recognized that host-virus interactions play a crucial role in the occurrence and pathogenesis of HAD. Microarray and high throughput genomic technologies have greatly facilitated the examination of this interaction. A panoply of host genes have been shown to be influenced by HIV infection that facilitate subversion and manipulation of the host immune system during HIV infection of the brain. To date, most studies exploring HAD pathogenesis
Recently, microRNAs (miRNAs), a class of small (20–25 nucleotides) non-coding RNAs, have been recognized as master post-transcriptional regulators of mRNAs
To date, a joint study of the genome wide mRNA and miRNA profiling based on HIV-infected brain tissue with and without dementia is still lacking, although some individual mRNA
A snapshot of DE mRNA and miRNA profiles between HAD and HIV non-demented brains
In order to identify the mRNA and miRNA, which may contribute to the pathogenesis of HAD, a parallel genome-wide mRNA and miRNA profiling of the frontal cortex from HIV patients with and without dementia was performed.
GenomeStudio was used to analyse the normalized mRNA dataset. We observed 468 statistically significant candidate genes that were differentially expressed between two groups (P<0.05) (see Additional file
Additional file 1
Table S1. DE gene list.
Click here for file
Additional file 2
Figure S1. Hierarchical clustering analysis of global mRNA and miRNA profiles obtained from the frontal cortex tissue at autopsy of HIV patients with and without dementia. Pearson correlation algorithm was chosen to evaluate and visualize the mRNA and miRNA expression patterns using GenomeStudio v3 and GeneSpring respectively. A. mRNA clustering. HAD samples are highlighted with (
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The analysis of miRNA data using GeneSpring identified 68 miRNA that were significantly differentially expressed in HAD and HIV non-dementia cortex (Additional file
Additional file 3
Table S2. DE miRNA list.
Click here for file
Additional file 4
Figure S2. Target of hsa-miR-137.
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Additional file 5
Table S3. Low and high stringency G-seed search for miR-137, miR-153 and miR-218 targets.
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Functional annotation of the mRNA DE genes and miRNA target genes was carried out in Database for Annotation, Visualization and Integrated Discovery (DAVID) together with extensive literature search. These mRNA DE genes were significantly associated with biological processes, such as cell death/cell cycle, neuronal processes, metabolism, transcriptional regulation, protein modification, signal transduction, and response to virus/stress, as shown in Figure
Additional file 6
Table S4: Gene sets significantly enriched by GSEA analysis.
Click here for file
Figure 1
Representation of gene ontology cellular components and biological processes of mRNA and miRNA profiles
Representation of gene ontology cellular components and biological processes of mRNA and miRNA profiles. A pie chart representation of biological processes and cellular components, where each part represents -log2 of the P-value of biological process and cellular component from the set of significant biological processes and cellular components. The total of -log2 of the P-value is 1. The P-values were retrieved from gene ontology analysis in DAVID. A. Biological processes of mRNA profiles. B. Biological processes of miRNA profiles. C. Cellular components of mRNA profiles. D. Cellular components of miRNA profiles.
Figure 2
Enrichment plots and heat maps for the gene sets of dendrite, ATPase activity coupled to ion transmembrane movement generated by GSEA
Enrichment plots and heat maps for the gene sets of dendrite, ATPase activity coupled to ion transmembrane movement and cytoskeleton dependent intracellular transport generated by GSEA. (A) Enrichment plot for the gene set of dendrite. Y-axis: value of the ranking metric; X-axis: the rank for all genes. Bottom: plot of the ranked list of all genes. Genes whose expression levels are most closely associated with the HAD or HIV non-dementia group are located at the left or right edge of the list while genes from the gene set dendrite within the ranked list are located at the middle. Top: the running enrichment score for the gene set as the analysis walks along the ranked list. The score at the peak of the plot is the enrichment score (ES) for this gene set and those genes appear before or at the peak are defined as core enrichment genes for this gene set. (B) Heat map of the core enrichment genes of the dendrite gene set, which contributes most to the ES. Rows: gene; columns: samples. Range of colors (red to blue) indicates the range of expression values (high to low). (C) Enrichment plot for the gene set of ATPase activity coupled to ion transmembrane movement. (D) Heat map of the core enrichment genes of the gene set ATPase activity coupled to ion transmembrane movement. (E) Enrichment plot for the gene set of cytoskeleton dependent intracellular transport. (F) Heat map of the core enrichment genes of the gene set cytoskeleton dependent intracellular transport.
Functional annotation results of miRNA target genes were consistent with that of mRNA results, but noteworthy is that the miRNA target genes showed relatively more comprehensive biological processes and cellular components, which could be attributed to the ability of single miRNA to have numerous mRNA targets, therefore further validation might be needed to precise the specificity. Cellular components of miRNA target genes are mainly distributed in cellular (61%) and neuronal related-structures (19%)(Figure
Quantitative real time RT-PCR corroboration of mRNA and miRNA array dataset
In order to confirm and validate the data obtained by both data sets, we analysed 11 DE genes and 6 DE miRNAs and 1 non-changed miRNA using quantitative real time RT-PCR on the same study sample set.
For mRNA, the quantitative real time RT-PCR for 9 of the 11 genes was fully consistent with their microarray expression profiles and trends (P<0.05) (Figure
Figure 3
Validation of mRNA and miRNA profiles using quantitative RT-PCR on the same sample set of microarray study
Validation of mRNA and miRNA profiles using quantitative RT-PCR on the same sample set of microarray study. A. mRNA profiles validation. B. miRNA profiles validation. Data was analysed using the 2-ΔΔCt method and results were plotted as fold differences of relative expression normalized to house keeping gene (GAPDH) and Hs_RNU6B_3 individually. The data have been collected from the same RNA samples from which mRNA and miRNA profiling have been done. All the data presented in this figure have significant P value of <0.05 as calculated by Student's t-test.
For miRNA, the quantitative RT-PCR for 7 of 7 miRNAs was consistent with the trend seen in microarray analysis (P<0.05) (Figure
Additional file 7
Table S5. Validation of non-changed miRNA.
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Pathway analysis of DE mRNAs and miRNAs
DE gene list and DE miRNA target gene list were uploaded to DAVID to explore functionally relevant pathways.
For mRNA, long-term potentiation, axon guidance and signalling pathways (including MAPK signalling pathway, calcium signalling pathway, Jak-STAT signalling pathway and VEGF signalling pathway) stood out significantly. Not surprisingly, we found 7 genes significantly dysregulated in long-term potentiation pathway (CHP, CACNA1C, ITPR1, MAP2K1, PPP1R12A, PPP3CA and RPS6KA1) (Additional file
Additional file 8
Figure S3. Significantly dysregulated genes on long term potentiation pathway in HAD brains compared to HIV non-dementia brains.
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Additional file 9
Figure S4. Western blot validation of MAP2K2 and MAP2K4. MAP2K2 and MAP2K4 encoding proteins MEK2 (A) and JNK (B) with relative molecular weight 45 and 46kD respectively were separated on 12% SDSpolyacrylamide gel and blotting with specific antibodies against them. Semiquantitative analysis has been carried out by comparing the relative protein level (standardized by Actin (C)) in HAD and HIV non-dementia patients. The quantification result demonstrated the trend similar to the one observed in microarray and qPCR for HAD patients when compared to HIV non-dementia patients.
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Additional file 10
Figure S5. Significantly dysregulated genes on calcium signalling pathway in HAD brains compared to HIV non-dementia brains.
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Additional file 11
Figure S6. Significantly dysregulated genes on Jak-STAT signalling pathway in HAD brains compared to HIV non-dementia brains.
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Additional file 12
Figure S7. Significantly dysregulated genes on VEGF signalling pathway in HAD brains compared to HIV non-dementia brains.
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Figure 4
Significantly down-regulated genes on axon guidance pathway in HAD brains compared to HIV non-dementia brains
Significantly down-regulated genes on axon guidance pathway in HAD brains compared to HIV non-dementia brains. Pathway analysis has revealed the significant involvement of axon guidance molecules and their receptors in HAD brains. The genes, which were found to differentially express between HAD versus non-demented patients in the current study are highlighted in green.
Figure 5
Significantly down-regulated genes on MAPK pathway in HAD brains compared to HIV non-dementia brains
Significantly down-regulated genes on MAPK pathway in HAD brains compared to HIV non-dementia brains. Pathway analysis has revealed the significant involvement of MAPK pathway in HAD brains. The genes, which were found to differentially express between HAD versus non-demented patients in the current study are highlighted in green. The rectangles represented members of Mitogen-activated protein kinases family while the ovals are related genes involved in MAPK pathway.
It is worth mentioning that most of the core-enriched genes contributing to each individual gene set significantly enriched in GSEA analysis fell into neurodegenerative disease related pathways, such as tight junction KEGG pathway, neurodegenerative disease pathway, MAPK signalling pathway, axon guidance pathway, and phosphorylative mechanisms/signalling pathway (for details see in Figure
For miRNA, a number of significantly involved pathways (P<0.05) were revealed, including signalling pathways, adhesion/junction, axon guidance, depression/potentiation, apoptosis/cell cycle, inflammation-related pathways, ubiquitin mediated proteolysis, and regulation of actin cytoskeleton (Additional file
Additional file 13
Table S6. DE miRNAs and their target pathways.
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Correlation between expression levels of DE miRNAs and DE mRNAs
We evaluated the significance levels of all possible correlations between DE mRNAs and miRNAs using SA-BNs
Figure 6
miRNA-mRNA correlation network
miRNA-mRNA correlation network. miRNA-mRNA correlation network discovered by SA-BNs, rendered in cytoscape
Figure 7
Pearson's correlation of miRNA-mRNA pairs vs. significant confidences of their interactions discovered by SA-BNs
Pearson's correlation of miRNA-mRNA pairs vs. significant confidences of their interactions discovered by SA-BNs. A: Depiction of positive interactions from each miRNA. B: Pearson's correlation of miRNA-mRNA pairs vs. significant confidences of their interactions discovered by SA-BNs. The X-axis shows the confidence of interactions, ranging from 0 (the least confident) to 1 (the most confident), while only the statistical significant scale remains (P<0.05). The Y-axis shows the sample correlations of identified miRNA-mRNA pairs. The red and blue dots are miRNA-mRNA pairs, which are correlated at the significant level (P<0.05). It shows that the identified interactions are largely correlated, either negatively or positively, suggesting direct interactions and indirect interactions correspondingly. C: Depiction of negative interactions from each miRNA ◊miR137 □miR153 OmiR218 ×miR299-5p *miR376a XmiR122 +miR592 –miR584 -Others.
To validate this correlation further, miRNA mimic of miR-137 and negative control treatments were carried out. That led a significant decrease in expression levels of NUFIB1, SLC, RNF, BAG4, SPRED, ZRANB at 24 h after transfection (Figure
Figure 8
Effect of the transfection of microRNA mimic of miR-137
Effect of the transfection of microRNA mimic of miR-137. A. Transfection of neuroblasts showing basal expression of miR-137, based on which our experiments on miR-137 target genes were carried out. B. Column chart of fold changes of expression values (qRT-PCR) between SH-SY5Y transfected with miR-137 mimic or miRNA mimic negative control. Values are based on fold change calculated from 2-ΔΔCt method. All the columns have significant P value of <0.05 as calculated by Student's t-test.
This is the first joint study of whole-genome mRNA and miRNA profiling using individual human brain RNA from autopsies of HAD and HIV non-dementia patients. In this study, we initially compared mRNA and miRNA data at the clustering, gene ontology (including cellular components and biological processes), and pathway levels. Following that, SA-BNs correlating miRNAs and mRNAs by their expression levels were performed to validate the accurate prediction of genes potentially targeted by dysregulated miRNAs.
The clustering and gene ontology results showed excellent functional concordance between mRNA and miRNA, demonstrating the significant involvement of neuronal cellular components and biological processes such as: signal transduction, transcriptional regulation, metabolism, response to stimuli, cell cycle/apoptosis, protein modification, neuronal processes and ion transport, respectively. This intrinsic functional consistency between miRNA and mRNA has given an extra power to our findings in relation to understanding the genomic basis of HIV neuropathogenesis and HIV-mediated neurodegeneration. Moreover, the DE miRNAs were more robust than their mRNA counterparts in providing a comprehensive snapshot of cellular components and biological processes involved in neuropathology and neurodegeneration. Compared to DE miRNAs, DE mRNAs could only predict elemental functional pathways and processes related to neuropathology. DE miRNAs revealed the participation of additional cellular components (eg. ion channel, actin cytoskeleton, tight junction) and biological processes (cell adhesion, tissue/embryonic development, learning), which also concurs with biological processes of mRNA. Interestingly, these findings are consistent with study, which has been done using CSF of HIVE patients
Pathway analysis of the joint mRNA and miRNA results provided the first
Moreover, our results highlight axon guidance downstream signalling pathways, which allow precise patterns of connectivity within the CNS. For instance, the MAPK pathway comes out significant in both our mRNA and miRNA profiling. Studies have shown that the activation of MAPK is necessary for axon guidance
In addition, endocytosis is another critical aspect of guidance receptor activation and signalling
Further, our miRNA and mRNA Bayesian correlation analysis has provided an unambiguous snapshot of miRNA and mRNA functional interactions and their biological significance. Sophisticated Bayesian Structure learning approach defines miRNA-mRNA interactions based on their relative expression of all of these molecules in each condition. This network-based approach identified these key interactions with very high confidence. These interactions define the network topography that is provided by Bayesian statistics and is substantially more rigorous than individual correlations that can be defined conventionally. These relationships, therefore, are more likely to be meaningful at the system level compared to reporter assay. We identified 195 positive and negative statistically significant correlations (P<0.05) between our DE mRNAs and miRNAs. It strengthens the correlation and shows the credibility of Bayesian analysis we have performed for establishing this intrinsic functional relationship between mRNA and miRNA. Among them, two thirds of functional correlations are from 5 DE miRNAs: miR-137, 153, 218, 376a and 299-5p. They all changed greater than 2 fold. Since all of them have been reported to be involved in classical neurodegeneration, for instance, miR-376a has been reported to mutate in Huntington's disease (HD) brains
Although our study is the first comprehensive parallel genome-wide mRNA and miRNA profiling of HIV infected human brains, there are still limitations: 1. In future, a bigger sample size, blood and CSF samples will be needed to further validate these findings, and confirm the clinical value of this findings; 2. These findings are based on the whole human brain cortex rather than specific cell types (eg. neurons) due to lack of plausible and effective methodologies for perfect cell separation. Although Laser Capture Microdissection (LCM) is currently used in studying cell types, there are significant limitations in profiling single cell type
Overall, our study provides a strong foundation and durable framework for systematic large-scale studies on HIV-infected adult brain to define functional genomic phenotypes of neurodegenerative diseases and functional networks between miRNA and mRNA, which may lead to the development of new generation of prognostic and diagnostic markers and therapeutic intervention strategies for viral and non-viral neurodegenerative diseases.
This study is the first report on whole-genome joint mRNA and miRNA profile analysis from individual native brain tissue from HIV+ patients with and without dementia and it underscores the important role of intrinsic functional correlation between mRNA-miRNA, which is closely tied to HIV-mediated neurodegeneration. Through mRNA and miRNA joint profiling this study has provided the first thorough
Brain tissue collection
Brain tissue samples were obtained from HIV-1-infected patients with or without dementia through the National Neuro-AIDS Tissue Consortium (NNTC, Request # R203) and the Westmead Hospital, Sydney, Australia (Reference No: 5465). Samples were collected at post-mortem with short delay. The autopsied brain tissue was snap frozen in liquid nitrogen and stored at −80°C until required for use. Frontal cortex of 10 male patients with HAD and 8 without were used for this study due to its importance in motor impairment and involvement in other neurodegenerative disorders, such as: AD
Additional file 14
Table S7. Patient details.
Click here for file
RNA isolation and mRNA and miRNA profiling
Total RNA was extracted from 30 mg of brain cortex tissue. Tissue samples were homogenised using a high-speed agitation polytron belnder (Kinematica, Luzern, Switzerland) in the presence of RNA lysis buffer (Qiagen miRNeasy Mini kit- Qiagen, USA). The RNA was isolated and purified with a miRNeasy Mini kit with DNAse I digestion on the column (Qiagen, USA) according to the manufacturer's protocol. The quality and quantitiy of the RNA preparations was assessed using an Agilent 2100 Bioanalyser (Agilent Technologies, USA). RNA integrity scores were>7 for all the samples analyzed. FirstChoice Human Brain Reference commercially available RNA (Ambion, Inc., USA) was used as a control RNA for the microarray analysis.
For mRNA profiling, cRNA amplification and labeling with biotin were performed using Illumina TotalPrep RNA amplification kit according to the manufacturer's directions (Ambion, USA) with 500ng total RNA as input material. cRNA yields were quantified using Agilent 2100 Bioanalyser. Gene expression analysis was performed using the Sentrix Human-6 v2 Expression BeadChip (Illumina, USA), and BeadStation system from Illumina as per manufacturer's instructions. The Human-6 v2 Expression BeadChip allows genome-wide expression profiling of more than 48,000 gene transcripts and known alternative splice variants from the RefSeq database. For miRNA profiling, 1000ng total RNA was labelled with FlashTag Biotin HSR RNA Labeling Kit (Genisphere Inc. USA) and analzed using Affymetrix GeneChip miRNA Array (Genisphere Inc. USA), which contains 1105
Gene expression data analysis was performed using GenomeStudio version 3 (Illumina Inc., Singapore). The gene expression data was normalised using the cubic spline function, the genes were selected if the detection P<0.01 (indicating the transcript was detected) in at least one group. All samples were coded and analyzed blindly to avoid any bias. The differential gene expression analysis was performed using Illumina custom error model with false discovery rate correction implemented in GenomeStudio
Real time quantitative RT-PCR validation of mRNA and miRNA
The data for mRNA and miRNA were selectively corroborated with real-time PCR to ascertain their expression trends. For mRNA, 5ng total RNA was reverse transcribed using oligo d(T) and Superscript III followed by RNase H treatment (Invitrogen Life Technologies), per manufacturer's instructions. PCR primers (Additional file
Additional file 15
Table S8. qPCR primers.
Click here for file
Transfection of microRNA mimic
SH-SY5Y cultures were maintained as confluent monolayers at 37°C with 5% CO2 and 90% humidity in SH-SY5Y media (DMEM with 10% (vol/vol) foetal calf serum, 20 mM HEPES, 2 mM L-glutamine). For differentiation cells were seeded at 4 × 104 cells/cm2 and treated with all-trans retinoic acid (ATRA) media (SH-SY5Y media with 10uM ATRA) for five days, followed by treatment in brain-derived neurotrophic factor (BDNF) media for three days (SH-SY5Y media without foetal calf serum and with 50 ng/ml BDNF)
Functional validation of proteins using western blot
Western blot was employed to validate part of the microarray data. 4 HAD patients and 4 HIV non-dementia patients' brain samples were used for validation of the microarray study by western blot analysis. Total cellular proteins were extracted as described before
Data analysis
For mRNA, gene ontology analysis has been carried out using DAVID and GSEA. Illumina ID of differential expressed genes was uploaded to the DAVID database and the analysis was performed using the algorithm within the softwares. With GSEA, the whole genome (27455 genes) with expression value were uploaded to the software and compared with catalog C5 gene ontology gene sets in MsigDB
Competing interests
The author(s) declare that they have no competing interests.
Authors' contributions
L Zhou fully performed the work, analyzed data and drafted the paper. GM Pupo assisted with RNA amplification and microarray hybridization. SL Tran and H Rizos provided full assistance with the WB validation analysis. B Wang assisted with real time RT-PCR and data analysis. R Rua participated in the RNA extraction and quantitation. NK Saksena conceived, designed, supervised and coordinated, along with providing assistance with drafting the manuscript; P Gupta and R Rahme provided assistance with miRNA extraction and miRNA Affymetrix array. M Cairns and B Liu assisted with bioinformatic and miRNA Affymetrix analyses. A Carroll provided assistance with miRNA knockdown experiment. Part of the work on miRNA reported herein was supported by the funding from the Judith Mason Foundation to NKS. All authors read and approved the final manuscript.
Authors thank NNTC for providing brain samples used for this study and Dr. Ashley Schoell (Los Angeles) for facilitating that. In addition, this publication was made possible from NIH funding through the NIMH and NINDS Institutes by the following grants: Manhattan HIV Brain Bank: U01MH083501, R24MH59724; Texas NeuroAIDS Research Center U01MH083507, R24 NS45491; National Neurological AIDS Bank 5U01MH083500, NS 38841; California NeuroAIDS Tissue Network U01MH083506, R24MH59745; and Statistics and Data Coordinating Center U01MH083545, N01MH32002. This study was funded by the World AIDS Foundation grant to NKS. HR is a recipient of a Cancer Institute New South Wales, Research Fellowship and a NHMRC Senior Research Fellowship. MC is supported by the Schizophrenia Research Institute and the NHMRC project grant (631057). BL is supported by the Neurobehavioural Genetics Unit and NSW Health.
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text:
Here are some givens about Republican primaries for president.
1. The Media tries, and for the most part, does, pick the most left leaning contender (from either Party).
2. The Republican Establishment tries to help the Media pick the most left leaning Republican contender.
3. If there is only one candidate who fits the standard Republican Establishment/Media candidate formula he is called "The Frontrunner."
4. Conservative Republicans cry foul if there is no conservative "frontrunner."
5. If by some stroke of luck there is one or more true conservative candidates in a Republican primary the same people who longed for one say, "Oh but he or she can't win."
6. During rigged debates, most Republican candidates get off message as soon as possible – and stay there.
7. Conservative Republicans who stay on message and are not afraid of Media slander and attacks – win.

subset: commoncrawl


text:
- English
- français
Oxygen Dissociation by Concerted Action of Di-Iron Centers in Metal-Organic Coordination Networks at Surfaces: Modeling Non-Heme Iron Enzymes
The high chemical reactivity of unsaturated metal sites is a key factor for the development of novel devices with applications in sensor engineering and catalysis. It is also central in the research for sustainable energy concepts, e.g., the efficient production and conversion of chemical fuels. Here, we study the process of oxygen dissociation by a surface-supported metal-organic network that displays close structural and functional analogies with the cofactors of non-heme enzymes. We synthesize a two-dimensional array of chemically active di-iron sites on a Cu(001) surface where molecular oxygen readily dissociates at room temperature. We provide an atomic-level structural and electronic characterization before and after reaction by combining scanning tunneling microscopy, X-ray absorption spectroscopy, and density functional theory. The latter identifies a novel mechanism for O-2 dissociation controlled by the cooperative catalytic action of two Fe2+ ions. The high structural flexibility of the organic ligands, the mobility of the metal centers, and the hydrogen bonding formation are shown to be essential for the functionality of these active centers allowing to mimick biologically relevant reactions in a confined environment.
- doi:10.1021/nl2031713
- View record in Web of Science
Keywords: Heterogeneous catalysis ; synthetic enzymes ; oxygen activation ; Stm ; Dft ; Soluble Methane Monooxygenase ; X-Ray-Absorption ; Ribonucleotide Reductase ; Dioxygen Activation ; Molecular Catalysts ; Oxidation Catalysis ; Crystal-Structures ; Cu(001) Surface ; Water ; Fe
Record created on 2012-06-12, modified on 2017-05-12


text:
- Anonym
- Tyskland, Kvinnelig, 18-24
- Novise-nomade
- 1 Gjennomgang
The stuff is really friendly and tries to help you, for a price (like if they drop you off and pick you up somewhere). You have to pay for everything extra, like wifi. The bathrooms are not clean and some you can't look. And There are busses outside, that are taking you to the city and if you decide to walk, it's a hour way. And the beds we stayed in where uncomfortable, you could feel the elastic springs in the mattresses. We were there for one night and that was enough!
Hostelworld mobilapper
Bestill mens du er på farten med de nye mobilappene til Hostelworld.


text:
Wednesday, December 31, 2014
WOYWW: Last Day of the Year!
19 blog hugs:
- Maxine D said...
Thank you Peggy for your sharing your beautiful art with us, and for your thoughtful and encouraging journalling. May you be richly blessed in 2015
- December 31, 2014 at 2:58 PM
- Nikki C said...
Happy new year and what a lovely drawing all the best in the new year hugs Nikki 4
- December 31, 2014 at 3:05 PM
- Shaz in Oz.CalligraphyCards said...
ah beautiful illustrations there as usual and lovely devotion, Peggy would love to come and colour with you your pencils are beautiful
every blessing on your 2015 and happy WOYWW!
Shaz in Oz.x #15 I think
- December 31, 2014 at 3:38 PM
- glendabrooks.blogspot.com said...
What a wonderful drawing you have created. Happy new year and many blessings being sent your way.
Glenda #24
- December 31, 2014 at 5:46 PM
- Annie said...
I am always thankful for what has happened in my life but rarely look back. I focus on making each day count and being as good a person as I can each day. Thankyou for your love and support through the past year.
Wishing you a very happy New year filled with many creatived times and lets hope it's a happy and healthy one for us all.
Annie x # 22
- December 31, 2014 at 5:50 PM
- sandra de said...
Wishing you a joyful new year and lots of fun at the beach.
Sandra de @31
- December 31, 2014 at 6:54 PM
- Robyn Oliver said...
Hi Peggy and may tomorrow be the start of a wonderful year. Lovely artwork Cheers RobynO#35
- December 31, 2014 at 9:10 PM
- Twiglet said...
Thanks for a good yearful of snooping round your crafty activities on your desk. All the best for 2015! x Jo
- December 31, 2014 at 9:29 PM
- Regina Hamilton said...
You journal page is stunning. Enjoy your vacation. Have a Happy New Year. #37
- December 31, 2014 at 9:55 PM
- Shel C said...
The faces on your drawing are so beautiful and serene. Love them! Happy New Year and happy WOYWW :) #39
- December 31, 2014 at 10:42 PM
- Anne said...
Beautiful work. Happy New Year. Anne x #28
- January 1, 2015 at 1:01 AM
- Alexandra said...
Beautiful work, Peggy. You create such beauty from colored pencils. Simply amazing. Happy New Year and all the best to come! :)
- January 1, 2015 at 1:55 AM
- Angie said...
Lovely drawing and lucky you to be on vacation!
Happy WOYWW!
Angie #56
- January 1, 2015 at 5:47 AM
- Chrysalis said...
Hi Peggy - a beach holiday sounds wonderful. Thanks for sharing your beautiful art work, and all the spiritual encouragement during the year. Hugs and blessings for 2015, Chris # 3
- January 2, 2015 at 3:24 AM
- Julia Dunnit said...
There is no escaping it Peggy, i will never stop being delighted by your art....even with a pot of coloured pencils yo you match the finest of oil paintings for me!
Enjoy your time at the beach, just what at the beach, and Happy New Year! Xxx
- January 2, 2015 at 3:56 AM
- Julia Dunnit said...
There is no escaping it Peggy, i will never stop being delighted by your art....even with a pot of coloured pencils yo you match the finest of oil paintings for me!
Enjoy your time at the beach, just what at the beach, and Happy New Year! Xxx
- January 2, 2015 at 3:56 AM
- Sharon said...
Hi, do you mind if I ask you what that book is? It looks wonderful. Your art is just beautiful!
- January 2, 2015 at 6:24 AM
- Shoshi said...
What a beautiful picture for your prayer journal, Peggy. Brilliant idea to take your coloured pencils out with you - so much more convenient than paint, and you can keep going with the art work even if you are out and about!
With the Lord, so often things are not as they appear, and only through the influence of the Holy Spirit can our eyes be opened to see things as God sees them. We so easily judge by external appearances, when these are so unimportant! God shows the heart.
Happy New Year, and may you continue to be blessed with creativity, to bring a blessing to others as you have been doing.
Happy WOYWW,
Shoshi #58
- January 2, 2015 at 9:06 AM
- Jo Ann said...
What a beautiful artwork this one is. Great coloring and message. Thanks a lot for joining our Challenge last week at Word Art Wednesday. There's still time to enter more projects in this week's Challenge so, I hope you come back and join us again. Want to share God's Word with you found in Psalm 27:14 which says, "Wait for the LORD; be strong and take heart and wait for the LORD." A Blessed and Happy New Year to you!
- January 5, 2015 at 6:25 PM


text:
Hey there, time traveller!
This article was published 10/9/2012 (1800 days ago), so information in it may no longer be current.
FARMLAND prices in Manitoba have soared to record highs in the wake of an unprecedented demand from expansion-hungry farmers eager to cash in on high grain prices, industry officials say.
In its 2012 Farm Edition report released Monday, RE/Max said productive grain land in southwestern Manitoba -- the only local area covered in the report -- is selling for an average of $1,350 an acre.
That up about 35 per cent from two years ago, when the average price was about $1,000 an acre, and up 286 per cent from 15 years ago, when it was about $350 an acre, said Ian Anderson, a real estate agent/appraiser with RE/Max Valleyview Realty in Brandon.
And the price escalation isn't limited to just the southwestern corner of the province.
"They (grain land prices) are going up all over Manitoba," said Stacey Hiebert, co-owner of Canada Farm Realty in Steinbach.
Hiebert said prices have been climbing by an average of about 10 per cent a year for the last three or four years in most areas of the province. In southeastern Manitoba it's been even more than that because there are more land-intensive livestock operations there, which drives up demand.
He said grain land there is selling for between $1,900 and $2,300 an acre.
Claude Jacques, a local appraiser with Farm Credit Canada (FCC), the country's leading agriculture lender, said a variety of factors are driving up land values, including rising demand, limited supply, low interest rates and high grain prices.
Jacques said FCC's fall Farm Land Values report, which will be released on Oct. 9, will look at how much prices have increased in different areas of the province.
"You've got some areas where price are significantly higher and others where it will be a little less."
The RE/Max report, which looked at prices in 16 markets across the country, said they're up almost everywhere while supply is tight across the board. It said selling prices per acre range from as low as $800 to $1,500 in northern Saskatchewan to as high as $40,000 to $60,000 in British Columbia's Fraser Valley.
The report said most farms don't even make it to the open market, with many being sold between neighbouring farmers. Those that do get to market usually sell in less than a month.
The report also said it's not just farmers who are snapping up farmland these days.
"Investment funds are taking a hard look at farmland... with well-known asset managers calling farm returns among the best investments out there," said Gurinder Sandhu, executive vice-president and regional director for RE/Max Ontario-Atlantic Canada.
But Sandhu said that hasn't provided farmers with added incentive to sell their land.
"In fact, the reverse is true, with many looking to further amass parcels of land and expanding existing operations."
Hiebert said that's certainly been the case this fall in Manitoba.
"Guys are hanging on to what they have. It (the value of farmland) is not going to go down, and the outlook for agriculture is good. So they want to keep it for a little bit longer."
Hiebert and Anderson also said while institutional investors may be snapping up farmland in other parts of the country, there's been little evidence of that happening in Manitoba.
$1,200 to $1,500 -- average selling price for grain land in southwestern Manitoba
$1,900 to $2,300 -- average selling price for land in southeastern Manitoba
$1,000 -- average price of grain land two years ago in southwestern Manitoba
35 -- percentage increase since then
$350 -- average selling price 15 years ago in southwestern Manitoba
286 -- percentage increase since then
$800 to $1,500 -- lowest average price per acre among 16 Canadian markets surveyed by Re/Max; that was in northern Saskatchewan
$40,000 to $60,000 -- highest average selling price per acre, in the Frazer Valley.
Sources: Re/Max, Re/Max agent/appraiser Ian Anderson, and Canada Farm Realty co-owner Stacey Hiebert


text:
I took your script and did the following with it:
for x in {1..10}; do
for i in {1..10000}mv $i.txt $i.tmp
# echo This is a test file > $i.txt
echo "Halfway through pass $x"
ls | wc -l
ls | sort -u | wc -l
sleep 2
for i in {1..10000}mv $i.tmp $i.txt
# echo This is a test file > $i.txt
echo "Done with pass $x"
ls | wc -l
ls | sort -u | wc -l
sleep 2
After 10 passes, there is no difference between "ls | wc –l" and "ls | sort –u | wc –l". I also bumped up the number of files from 1k to 10k. So I'm still looking for a way to reliably reproduce what has been intermittent here.
On 11/17/10 12:16 PM, "Jason Kendall" <email@example.com> wrote:
I wrote two scripts - and actually, I was able to trigger it by just making the files with this:
for i in {1..1000}
echo This is a test file > $i.txt
The second is basicly the same, just moving the file to a new name, but it doesn't look like that is required.
Seems any change to the directory list tables can trigger this.
On 10-11-17 03:11 PM, Brian Stamper wrote:
Re: NFS duplicate filenamesWhat thresholds are you using to reliably reproduce the issue? We only get periodic file deliveries, so we see this once a month or so. How many files definitely reproduce the issue, and does their size matter or is it specifically number? I can attempt to reproduce here against a variety of nfs servers.
On 11/17/10 12:06 PM, "Jason Kendall" <email@example.com> wrote:
Just tried it on an older box I have here: 2.6.19-skas3-v9-pre9 (client) connecting to my drobo running 18.104.22.168 (Custom) and had the same issue.
So I am again, back to square one.. But with a second person having the same issue.
On 10-11-17 02:37 PM, Brian Stamper wrote:
Re: NFS duplicate filenames Centos nfs4 server - 2.6.18-194.11.3.el5
RHEL nfs3 server - 2.6.18-194.17.1.el5
Fedora 13 client - 184.108.40.206-56.fc13.x86_64
I'm not registered on the Ubuntu support site, I found your issue via google.
On 11/17/10 11:26 AM, "Jason Kendall" <email@example.com> wrote:
Damnit.. That means its no longer my Drobo causing the issue.
Could you layout the clients with kernel versions please? I'd like to go back to tracking this down. Maybe send it to me and the ticket. Your the first person to come forward with the same issue.
On Wed, Nov 17, 2010 at 2:23 PM, Brian Stamper <email@example.com> wrote:
Varies actually, we've seen it on Fedora 13 clients connecting to a CentOS 5.5 server via nfsv4 and those same clients connecting to RHEL 5.5 server via nfsv3
On 11/17/10 11:20 AM, "Jason Kendall" <firstname.lastname@example.com <http://email@example.com> > wrote:
Interesting.. what is your backend? (NFS Server?)
On Tue, Nov 16, 2010 at 11:56 AM, Brian Stamper <firstname.lastname@example.com <http://email@example.com> > wrote:
Did you ever find an answer for this issue? I'm running into the same thing on Fedora 13 and yours is the only report of it I've seen. Same situation, I have an incoming directory with thousands of files that users attempt to mv, resulting in duplicate filenames showing up then disappearing eventually on their own. One difference I've seen is that if I mv/rename a file, it is listed singular, but if I rename it back, it's once again duplicate, at least temporarily.
If you got any further diagnosis or answers on this, I'd appreciate any info you have.


text:
Lectro: Well, let's see who got voted off! (Looks through votes) The votes seem pretty diverse so far. Bombell, Speedy, (then begins to look worried) Speedy, Speedy, Speedy, Speedy, um.... Well, it looks like we've designated our eliminatee for this round.
Speedy: Very well. I might see you chaps around someday, but for now I'll-
Alice: WAIT SPEEDY YOU TALK!?!???!??!?! Oh, how could I have been so ignorant! All this time I was merely pretending to understand you! I'm so sorry-
(Speedy gets launched out of the mansion via a spring.)
Alice: NOOOOOOOOOOOOOOOOOOOOOOOOOOO!
Base of the Dork Team
(Jake is seen writing some notes on a piece of paper.)
Data: That was awesome back there during the challenge! *notices that Jake is troubled* What's going on?
Jake: There is obviously a greater scheme about this "competition" thing. Especially considering-
Takeo: I AM GOING TO RIP THAT ELECTRIC FUCKERS BONES OUT AND GRIND HIM TO BITS.
Fyre: same.
Jake: -that. Anyway, we need to make sure we can solve this problem. However, the other team probably doesn't know what's going on. So in order to get answers, we'll have to win in any way possible.
Mika Sho: You don't mean we'll have to resort to-
Jake: Cheating? Undoubtably. Get ready for the next challenge, teammates. It's gonna be a big one.
(Camera pans to a top secret camera that none of the team members noticed as the episode ends.)


text:
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Tag a photo on or for a chance to be featured in our gallery.


text:
Takata Aware of Airbag Defect Since 1995
According to the Associated Press (AP), The New York Times reviewed Takata patent documents that indicate the company has been aware of the airbag defect that has been associated with at least five deaths and multiple injuries for years.
About the Takata Air Bag Recall
- In April 2013, Takata announced malfunction of the airbag inflator that may cause the airbag to deploy incorrectly, causing a safety hazard of metal shards shooting at the passenger(s).
- Over 16 million vehicles have been recalled from 11 automakers around the world.
- Takata is suspected to have been aware of the defect for over a decade before reporting it to a federal safety agency.
- The airbags were initially suspected to deteriorate and cause a safety hazard in highly humid areas.
- Tension is rising between Takata and The National Highway Traffic Administration (NHTA) since the supplier resisted a mandated recall expansion.
- The NHTA and U.S. Department of Justice are currently investigating the allegedly delayed airbag recall.
Takata Patent Documents
The New York Times reviewed patent documents submitted by Takata concerning the airbag defect that date back decades—all the way to 1995. The 1995 patent application expresses Takata's concerns about the malfunction of the compound within airbag propellant, ammonium nitrate. The patent outlines Takata's worry that the compound casing reacted to temperature changes, noting that it may explode under humidity. Although the company continues to state that the compound is not dangerous, documents indicate their exploration of its vulnerability to high temperature.
Takata has also stated that they have continued to investigate the root of the airbag malfunction. However, they are contradicted by patent documents from 2007 that warn the compounds susceptibility to "expand and contract and chance shape resulting in growth and cracking," providing a safety hazard with the risk that the inflator could "blow up because of excess pressure generated."
The National Highway Traffic Safety Administration (NHTSA) is taking allegations of the recall delay very seriously, requiring that Takata provide a history of any changes made to the compound in the past two decades.


text:
I met this guy while having a girls night out, brief encounter, chatted and stuff while I was visiting a different province. Once I was back home a few weeks later he emails me, we chat for a few weeks every day. Than start talking via fb and finally text message. It died down after 2 months. I deactivated fb for a bit than when I returned he had deactivated to and while this happen our texting stop (just for background info)
We originally started talking because we had both made plans to move to Hali in Jan toattend school for the same program. Which was kinda cool. I've finally moved here a few days ago and I was wondering if it would be weird if I emailed him to see what's up?
I don't know to many people neither does he (except me.) What should I say? Brief and simple
Most Helpful Guy
What That-Guy said was spot-on. You're a beautiful lady and if the guy wanted to talk with you and get to know you for a relationship, he would generally follow through on it. Some guys might be hesitant and intimidated by someone who's really pretty, but the fact you guys already broke the ice and already talked a lot, that's out of the question.
If he wants to be in a relationship or date you with hopes for more in the future, he will let you know. If he doesn't, then don't try to wait because that would hold you back from meeting other good men out there.22|00|0


text:
We're all seniors by the way. These group of guys that sit across from me always stare at me. Its a whole section of guys that constantly look at me. (About 8 guys). We have a seating chart and, at times, a couple of the guys would take turns sitting next to me and pull their chair near mine and glance at me throughout the whole period.
One even sat next to me and said out loud "this is the best seat in the class" and looked at me and yes everyone heard. It's really making me uncomfortable since they do it all the time. They're constantly watching me. It doesn't help that Im shy in that class and those guys are the loudest group in our class. One of the guys was sitting really close to me and pointed at me when a friend of his was staring at us. He moved his hand away when I saw him.
When we have class discussions and when one of the guys talk they look directly at me as if he's only talking to me. people have noticed that and just look at us. They know my name and everything, I honestly don't even know most of theirs. Are they just trying to mess around with me? Advice on how to stop feeling uncomfortable?
Most Helpful Guy
Talk to your teacher privately and get moved to a different area. Otherwise, play mind games with them by flirting and then acting like you don't even care who they are.00|00|0
I was thinking about talking to her but im not in the best terms with her sooo i really don't want to bother her. Haha that's really funny but i think ill pass on that
Most Helpful Girl
You could be imagining things, I always thought some guy stared at me in class, but sometimes its your imagination. I also notice that when i think someone is staring at me I glance at them for real (not just the corner of my eye) and they're actually not staring at me (just the position of their head looks like it). But once again, some people do stare and i've also had it happen quite often. If you actually see them staring at you and pointing at you they probably think you're pretty, and its normal for them to know your name, they're in your class. If its specifically one group constantly i'd be kind of creeped out though, just ignore them.00|00|0
Haha yeah I thought it was all in ny head but it's been going on since the first day. I've caught them staring at me more than a few times. Whenever I do they always fix their hair and smile at me then look down. It was this guy's birthday (part of the group of guys who look at me) and Im not joking when I say that he was just looking directly at me Nd nobody else when they were singing him happy birthday. There was nobody in front of me. people were looking at the two of us including th teacher. Yup it was awkward 😂😂
Oh God 😂😁 that is really awkward. If you catch them staring at you again, just legit tilt your head out and look them right in the eye and give them that look like "Um, are you going to keep looking at me, or?" (Works every time for me lmao). If it keeps happening just roll your eyes and sigh because it tells them you're really annoyed. Or if they sit next to you be like "i'm saving this seat for my friend, sorry", even if they know its a lie do it anyway, at that point, being rude doesn't matter anymore if they're creeping you out lol.


text:
With 80 years of heritage, Italian label René Caovilla conceives must-have shoes and accessories with strong charisma, unparalleled creativity and outstanding care. The brand's creations bring the Venetian shoemaking art to its enthusiasts through precious handmade details, contemporary shapes and a luxury aesthetic. Using distinctive shapes, textiles and exquisite embroideries, René Caovilla has garnered many an A-list fan including Beyoncè, Heidi Klum and Christina Hendricks.
No products were found matching your selection.


text:
JUVENILE DIABETES RESEARCH FOUNDATION Hope GalaMay 29th, 2015
Jim Cavanaugh, show more of Jim Cavanaugh photos or correct name Laura Cavanaugh, show more of Laura Cavanaugh photos or correct name Denise Fong, show more of Denise Fong photos or correct name Lane Murchison show more of Lane Murchison photos or correct name
For a commercial or editorial license, please email firstname.lastname@example.com.
Perfect for printing with no watermark up to 8x12. If you need something larger, we can usually do a custom print.
Perfect for screen and social, but not great to print. There is a small watermark in the lower right corner (you can crop it out).


text:
The parents of a 5-year-old Jackson, Ohio, girl who was previously reported missing made their first court appearance since they were arrested and charged with her murder. The police announced on Tuesday evening that the body of the little girl had been found hidden in the family's restaurant.
A judge at the Massillon Municipal Court set a bond of $5 million for 29-year-old Mingming Chen and 34-year-old Liang J. Zhao. The couple are both from the Jackson Township and stand accused of murdering their 5-year-old daughter, Ashley Zhao, who they had reported missing late Monday night. For the part she played in the death of her daughter, Chen will be charged with murder while the father, Liang Zhao, is charged with complicity to commit murder, both of which are first-degree felonies.
Missing Ohio 5-Year-Old Ashley Zhao Found Dead And 'Concealed' In Family Restaurant, Mother Charged With Murder https://t.co/jugwVwMx9G
— Inquisitr News (@theinquisitr) January 11, 2017
Tulsa World wrote that Ashley's mother is also charged with felonious assault for punching the child before her death while Zhao is charged with complicity to commit felonious assault. Both of Ashley Zhao's parents are being held at the Stark County Jail on a $5 million bond.
It was after an exhaustive day-long search that police announced on Tuesday evening that they had discovered the body of the missing girl. Ashley Zhao had been killed and hidden inside of her father's restaurant, Ang's Asian Cuisine at 4924 Portage St. NW in North Canton. The police did not release the details of exactly how the couple hid their murdered daughter in the family restaurant, however.
Parents of Ashley Zhao appear in court: According to investigators, Ashley Zhao died when Mingming Chen allegedly struck the child's head… pic.twitter.com/8wNSGJdblc
— CavanaughNancy (@CavanaughNancy1) January 11, 2017
Initial reports from the parents of Ashley Zhao had been that she had gone missing after being sent to take a nap in the back of the restaurant at around 4:30 to 5 p.m. on Monday evening. Chen called in with the missing report at approximately 9 p.m. Jackson Police Chief Mark Brink said that Ashley Zhao had been known to walk out of the back door on previous visits to the restaurant, but no one saw her leave in this instance. Jackson Township police even took to social media in their quest to find the missing girl, and a statewide endangered child advisory was issued.
Police now say that Mingming Chen struck her 5-year-old daughter in the face several times on Monday. The Stark County Sheriff's Office indicated that after being punching by her mother, a green fluid had been coming from the girl's mouth which her father noticed and washed away. The court documents go on to say that Zhao said that once he had washed up Ashley in the bathroom he noticed that she had stopped breathing. He reportedly laid her on the floor and tried unsuccessfully to revive his daughter by performing CPR. It is unclear if the father had been present when Mingming Chen was allegedly punching the girl, nor is it said where the beating took place. The investigation is ongoing.
The memorial at Ang's Asian Cuisine is growing for 5-year-old Ashley Zhao who was found dead inside the building. @wkyc pic.twitter.com/OgOFDD66jk
— Amani Abraham (@AmaniAbraham) January 11, 2017
Liang J. Zhao and Mingming Chen also have another child apart from Ashley, a 6-year-old daughter who has not been named due to the current circumstances. The little girl has been taken into the custody of Stark County Job and Family Services.
Cantonrep.com reported that though the $5 million bond may seem a bit high, Judge Eddie Elum explained that it was set with consideration of the fact that one of the defendants, Chen, could be facing deportation. While Liang Zhao is a U.S. citizen, he is known to have relatives in New York and both are flight risks. The couple will have their preliminary hearings at the Massillon Municipal Court once more on January 17 at 11 a.m. An autopsy is scheduled for Ashley Zhao today.
The non-profit riders club, Unforgiven, will be hosting a candlelight vigil for Ashley Zhao on Wednesday at 7 p.m. in front of Ang's restaurant and are asking persons to show their support to end child abuse.
[Featured Image by Stark County Sheriff's Department/Jackson Township Police]


text:
One of the most famous moments of the 2012 vice presidential debate between Joe Biden and Paul Ryan came when Ryan attempted to defend his tax plan. Debate moderator Martha Raddatz pressed GOP vice presidential nominee to provide specifics on how he and presidential nominee Mitt Romney would pay for their tax proposal.
While Ryan was trying to answer the question (without going into many details) Biden argued that it was not mathematically possible. However, Ryan remained firm on his stance saying Kennedy had done it.
BIDEN: It has never been done before.
RYAN: It's been done a couple of times.
BIDEN: It has never been done before.
RYAN: Jack Kennedy lowered tax rates and increased growth. Ronald Reagan --
BIDEN: Oh, now you're Jack Kennedy.
RYAN: Ronald Reagan --
BIDEN: This is amazing.
A similar incident and perhaps the most famous vice presidential moment in history took place in 1988 when Democrat Lloyd Bentsen mocked the young Republican Dan Quayle when he compared himself to John F. Kennedy.
Biden attempted to do something similar this time, for old time sake.


text:
A & Furst18K Gold, Pink Sapphire, and Diamond "Bouquet" Pendant Necklace
Stunning necklace in a bright color combination resembling a beautiful spring bouquet. Constructed of 18k yellow gold. Two delicate rolo chains sprinkled with stations of sparkling gemstones. Circular pendant featuring a glimmering, clustered mix of citrine, pink sapphire, rose de france, pink tourmaline, spessartite garnet, and diamonds. Lobster clasp closure. Please note this new item comes to you directly from a high-end boutique. Retails for $8,100.
Made in: Unknown Color: Gold, Pink, Orange Fabric Content: 18K Yellow Gold and Gemstones Condition: New with tags. No visible signs of wear from trying on.
Measurements & Sizing
- Total Length: 17"
- Necklace Drop: 8"
- Pendant Height: 1.25"
- Pendant Length: 1.25"
Returns & Shipping
Luxury Garage Sale guarantees that all items sold on our site are 100% authentic. All products we accept are inspected for brand-specific guidelines and are put through a rigorous authenticity test process.
LGS is not affiliated with A & Furst. We guarantee this A & Furst item to be authentic. A & Furst® is a registered trademark of A & Furst.
Item #: 142045


text:
Mobile Photography: Creating Consistent Look and Feel
Joe Greer, Photographer
- 0.5x
- 1x (Normal)
- 1.25x
- 1.5x
- 2x
Approach to Photography4:44
Favorite Instagram Shots6:12
Shooting at Columbia River Gorge2:23
About This Class
Photographer Joe Greer (@ioegreer) is known for capturing stunning landscape and portrait photography with movement, authenticity, and warm and inviting colors. How does he achieve that consistent look and feel?
In this 35-minute class, you'll join Joe in Portland, Oregon as he shares his approach to photography, goes shooting in the Columbia River Gorge, and shares invaluable, tactical tips for post-production mobile editing with apps like VSCO.
Inspired by Joe's process and passion for photography, you'll leave this class ready to capture your own incredible group of images. Share them with the class, share them on Instagram, and be proud of crafting a consistent look and feel that you can call your own.
Photographs by Joe Greer
Class Projects 71 See All
329 of 347 students recommendSee All
Joe Greer is a freelance photographer who found his creative journey beginning in Eastern Washington two years ago while attending Bible College. He specializes in landscape, portraiture, lifestyle, and adventure photography.
Joe has a sincere passion for cultivating community, making adventurous memories, and capturing moments that normally go unseen. His journey into photography started as a casual hobby and quickly became a passionate pursuit. For Joe, it became a way to communicate a message, to tell a story, to capture beautiful moments, and to share curated experiences.
Living in the Pacific Northwest has helped refine his eye for photography, while gaining experience and knowledge from a professional work environment at Visual Supply Company. Prepared to embrace community, culture, and any creative group to produce beautiful and original work.
Contact: [email protected]


text:
login with
To Dylan,
times will get hard. You are 16 now and ready to be on your own. Since day one you have known more facts and general information than me or your father and we are so proud of you baby boy ;) your girlfriend Lexi is lucky to have you ;) treat her with respect my dear I love you happy 16th ;)
Happy birthday to Dylan!! Are you guys doing anything special today to celebrate his big day?
Yes ladies :) he is such a bright young man I am so proud of all his achievements x We are going to eat out just the fam and Lexi. SO EXCITEDD!!!
That's so sweet! He sounds like a very nice young man :)
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About Barbara Canterbury
Birth: July 04
On Moms.com since: May 13, 2014
I am a mother of two hoping another. My husband Derek is a builder and I am a architect, and we hope to "build" a beautiful family together. Good one BarBar. I would love to talk to all you ladies! My life in Ras al Khaimah is luxurious! :)
All Recent Barbara's Posts


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Product Spotlight
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Earnings Roundup
Sunoco, Delek, Susser, Alon and Western Refining report strong third quarters.
NEW YORK – Third quarter results are in for many convenience retailers and petroleum marketers, and companies are overwhelmingly reporting positive results in the wake of increased refining and retailing margins.
Sunoco Inc
Sunoco Inc., headquartered in Philadelphia, reported net income of $549 million for the third quarter of 2008, an increase of 154 percent from a year-ago. Excluding special items, Sunoco's income for the 2008 third quarter was $559 million.
For the first nine months of 2008, Sunoco reported net income of $572 million, a decline of 36 percent. Excluding special items, income in the first nine months of 2008 was $561 million, a decline of 31 percent.
"After an extremely challenging market environment in the first half of 2008, very strong margins in our refining and supply and retail marketing businesses led to a record result in the third quarter," said Lynn Elsenhans, Sunoco's CEO and president.
Demonstrating the diversity of its earnings base, Sunoco's non-refining businesses earned $140 million during the third quarter – its highest quarterly contribution ever.
Retail marketing earned $72 million in the third quarter, a 132 percent increase over a year ago, despite lower year-over-year sales volumes, as falling wholesale gasoline prices through most of the quarter led to expanded retail margins. Results for the quarter included a $5 million after-tax charge related to asset impairment losses and associated costs in conjunction with the company's Retail Portfolio Management (RPM) program. The company anticipates future gains from the divestment of sites will exceed the impairment losses and associated costs recognized during the quarter.
Delek US Holdings Inc.
Delek US Holdings, based in Brentwood, Tenn., reported total revenue of $1.46 billion for the three months ended Sept. 30 -- an increase of 37 percent over the year ago period. Third quarter 2008 net income increased 24 percent to $25.4 million, driven principally by improved profitability in the retail and refining segments.
"Our third quarter 2008 contribution margin grew by 25 percent compared to the year-ago period, supported by a continued downward trend in commodities prices," Uzi Yemin, president and CEO of Delek US, said in a released statement. "Our Tyler refinery remained fully operational in the wake of Hurricanes Ike and Gustav, allowing us to benefit from elevated Gulf Coast pricing in early September. While hurricane-related fuel shortages impacted retail sales volumes in core regional markets late in the quarter, a general trend of declining wholesale fuel costs contributed to higher fuel margins in August and September, resulting in improved profitability at our retail subsidiary."
The retail segment contribution margin increased 12 percent to $23.7 million in the third quarter of 2008. Although hurricane-related supply shortages adversely impacted fuel sales at many retail locations during September, Delek US benefited from higher fuel margins throughout the third quarter, which served to more than offset lower sales volumes in the period.
Retail fuel margin increased on a year-over-year basis by 8.7 cents per gallon to 23.9 cents per gallon during the third quarter of 2008, serving to partially offset an 8.9 percent same-store decline in the total number of retail gallons sold in the quarter. The increase in fuel margins is mainly attributable to a favorable spread between wholesale and retail fuel prices in the quarter, in addition to favorable blending economics associated with the company's ongoing E10 (ethanol) blended fuel program.
For the three months ended Sept. 30, Delek US reported a 7.6 percent same-store merchandise sales decline, primarily attributable to regional fuel supply shortages during September and a reduction in discretionary consumer spending. The decline in same-store merchandise sales was partially offset by an increase in merchandise margin at 32.4 percent, compared to merchandise margin of 31.9 percent in the year-ago period.
Delek's retail segment markets gasoline, diesel and other refined petroleum products and convenience merchandise through a network of company-operated retail fuel and convenience stores under the MAPCO Express, MAPCO Mart, East Coast, Discount Food Mart, Fast Food and Fuel, and Favorite Markets brand names.
Susser Holdings
Corpus Christi, Texas-based Susser Holdings Corp. reported third quarter 2008 merchandise sales -- including sales from the company's Stripes stores and the Town & Country stores it acquired in November 2007 -- increased by 74.9 percent to $189.3 million vs. the same quarter a year ago for stand-alone Susser operations.
Total revenues for the combined organization were $1.2 billion, an increase of 80.1 percent from the third quarter of last year on a reported basis, and up 32.2 percent pro forma, assuming the Town & Country acquisition had taken place on Jan. 1, 2007. Gross profit was $118.8 million, up 78.7 percent on a reported basis and up 13.5 percent on a pro forma basis vs. a year ago.
Net income was $6.9 million, a 44 percent increase over the year-ago quarter. Pro-forma net income in the 2007 period was $5.5 million, taking into account the Town & Country acquisition and the related impact on Susser's tax position.
The increase in net earnings was driven by the contribution from new stores -- including Town & Country stores -- and higher merchandise and fuel margins, partly offset by higher interest, utilities, credit card, hurricane-related impacts and income tax expense.
"The 6.7 percent pro forma same-store merchandise sales increase and the 34.9 percent merchandise margin we realized in the third quarter demonstrate the continued vitality of the consumer economies in the primary markets we serve," said Sam L. Susser, Susser Holdings president and CEO. "During the quarter, we saw very strong performance from both the legacy Stripes stores in South Texas and Oklahoma, and even stronger performance from the newly acquired Town & Country stores in West Texas and Eastern New Mexico. We believe this performance reflects the continued positive impact of improved execution by our team on our merchandise and customer-facing initiatives, and a regional economy that continues to outperform national trends.
"Although consumers continued to reduce their driving in the face of record fuel prices over the summer, the impact was more than offset by higher fuel margins per gallon," Susser continued. "Retail margins averaged 22.2 cents per gallon -- up more than 3 cents from a year ago on a pro-forma basis. While the third quarter is generally one of our most profitable for fuel sales, this year's margins were bolstered by falling oil prices, which tends to improve our margins," he stated.
The company opened one new large retail unit in the Rio Grande Valley of Texas and closed another low-volume store in Corpus Christi during the third quarter, leaving the total store count at 509 as of Sept. 30, with 287 in-store restaurants. Two more stores with restaurants opened in the fourth quarter, and a Laredo Taco Company restaurant was added to another existing store. Four more stores are now under construction.
Convenience store merchandise sales were $189.3 million during the third quarter, up 74.9 percent on a reported basis and 10.4 percent on a pro forma basis. Same-store merchandise sales increased 4.8 percent in the third quarter on a reported basis, or 6.7 percent pro forma. Sales growth was driven primarily by strong increases in the sales of cigarettes, foodservice and beer. Total merchandise gross profit, net of shortages, was $66 million, up 83.6 percent on a reported basis and 16.1 percent pro forma.
Net merchandise margin increased to 34.9 percent for the quarter -- up from 33.2 percent on a reported basis for the third quarter of last year and 33.1 percent on a pro forma basis.
Retail fuel volumes increased to 163.4 million gallons for the quarter, up 50.1 percent on a reported basis, but down 3.6 percent on a pro forma basis. Average gallons sold per retail location were 325,900, which was down 2.3 percent from the third quarter of last year on a reported basis and down 6.2 percent on a pro forma basis. The volume decline reflects the impact of significantly higher pump prices versus a year ago that reduced fuel demand, especially near the Texas-Mexico border.
Retail fuel revenues increased to $619.7 million, up 112.7 percent on a reported basis and 32.9 percent on a pro forma basis. Fuel revenues were driven by the Town & Country contribution and by a $1.11-per-gallon increase in the retail price of fuel vs. a year ago, or $1.04 on a pro forma basis.
Retail gross margins for fuel increased 6.4 cents per gallon to 22.2 cents on a reported basis and by 3.3 cents a gallon pro forma. Retail fuel gross profit totaled $36.3 million, up 110.4 percent on a reported basis and 13.2 percent pro forma. Susser reports retail fuel margins before credit-card and other fuel-related expenses.
Credit-card fees in the third quarter were approximately 5.2 cents per gallon, vs. 3.4 cents per gallon a year ago on a pro forma basis, or an increase of $2.8 million. Utility costs, which are also tied to the cost of energy, were up $3.4 million on a pro forma basis.
For the first nine months of 2008, Susser reported merchandise sales of $545.9 million, an increase of 77.5 percent on a reported basis and 12.2 percent pro forma, vs. the comparable period a year ago. Same store sales increased by 6.7 percent, or 8 percent on a pro forma basis.
Alon USA
Alon USA Energy Inc., based in Dallas, reported its net income for the third quarter 2008 was $37.3 million, a 196 percent increase over the same period last year. Excluding special items, Alon recorded net income of $24.3 million for the third quarter of 2008, a 104 percent gain over the same period last year.
Net income for the nine months ended Sept. 30 was $21.9 million, an 84 percent decline compared to the same period last year. Excluding special items, Alon recorded a net loss of $60.6 million for the nine months ended Sept. 30, compared to net income of $141.0 million for the same period last year.
"We successfully completed the acquisition of the Krotz Springs, La. refinery this quarter. With the completion of the acquisition, our crude oil refining capacity increased by 50 percent to approximately 250,000 barrels per day (bpd), including four refineries located on the West Coast, West Texas and Gulf Coast. I am also pleased that we have completed work on the Fluid Catalytic Cracking Unit (FCCU) at our Big Spring refinery that was damaged in the Feb. 18, 2008 fire. With the completion of this work, we were able to restart the FCCU and return to our normal operating capabilities. We are grateful for the support of our insurers who have already advanced us $280 million to date," Jeff Morris, Alon's president and CEO, commented in the earnings statement.
"At our California refineries, we optimized our refining economics during the third quarter of 2008, lowering throughput rates to balance production with demand for our asphalt products. We also faced challenges during the quarter at our Krotz Springs refinery with Hurricanes Gustav and Ike. These hurricanes caused minimal damage to the refinery, but disrupted crude supply receipts into the refinery and product movements from the refinery while power was being restored to the area."
Alon USA owns four crude oil refineries in Texas, California, Louisiana and Oregon, with an aggregate crude oil throughput capacity of approximately 250,000 bpd. The company markets gasoline and diesel products under the FINA brand name and also operates more than 300 convenience stores primarily in West Texas and New Mexico, substantially under the 7-Eleven and FINA brand names. Alon supplies motor fuels to these stores primarily from its Big Spring refinery. In addition, the company supplies approximately 800 additional FINA-branded stations.
Western Refining
El Paso, Texas-based Western Refining Inc. reported third quarter 2008 net income of $109.2 million, a 134 percent increase over the year-ago period. For the first nine months of 2008, the company reported net earnings of $77 million, a decline of 71 percent from the first nine months of 2007.
The improvement in operating income in the quarter was due to higher refined product margins. The increase in margins was primarily the result of an increase in the amount of lower-cost crude oil processed at the company's refineries during the quarter. Margins also improved as the cost of crude oil declined faster than the prices of finished products.
"We are pleased with our third quarter financial results. Although Hurricanes Gustav and Ike had a positive impact on margins in the quarter, the actions we have taken to improve and enhance our Four Corners and Yorktown refineries contributed significantly to earnings in the quarter and should continue to do so in future quarters," Paul Foster, Western's president and chief executive officer stated.
Commenting on the fourth quarter, Foster said, "Despite the ongoing economic uncertainty, refining margins remained strong at our four refineries throughout the month of October. In fact, our combined refining gross margin for the month exceeded the third quarter level of $15.03 per barrel. Our retail and wholesale operations also posted strong financial results for the month. Looking at the remainder of the quarter, distillate margins look good as a result of steady demand and continued tight supply. While gasoline demand has declined the last couple of months, we believe the significantly lower prices at the pump today could stimulate demand and improve gasoline margins."
Western has a refinery in El Paso, two refineries in the Four Corners region of northern New Mexico and a refinery in Yorktown, Va. Western's asset portfolio also includes retail service stations and convenience stores in Arizona, Colorado and New Mexico.


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With over 200 years of collective experience, Hobson Insurance provides individuals and businesses in Hobson and surrounding areas throughout Montana and the entire United States with insurance solutions to protect their families and their companies. We are located in a small town of 250 people yet we provide customized insurance solutions for hundreds of clients across the country.
We specialize in national program business and are endorsed by 13 national buying groups, associations and franchises. Our specialty coverage has allowed us to establish long-standing relationships with multiple carriers who provide our clients with specialized options on packages with excellent rates.
In addition to our traditional insurance offerings like home, auto, commercial insurance, and workers compensation, we have special coverage for our national program businesses that include sporting goods stores, gun stores and ranges, archery shops and ranges, shoe retailers and pedorthists, clothing stores, athletic dealers, hardware stores, variety and gift stores, durable medical equipment dealers, and more. For these special programs, we provide one of a kind coverage to keep them secure.
Our staff is highly educated and holds multiple designations that give them the skillset to create policies to fit your personal and commercial needs. Not only does our staff hold professional designations such as CIC, CPCU, and CISR. Several of our staff have won presigious insurance awards on a national level.
We are a family-owned and operated agency, and make ourselves accessible to our clients when they need us. We work to keep our staff and our clients happy and extend ourselves to the community through local organizations. Call or contact us to learn more about our agency, to get started on a policy, request a quote.


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SALE Now On! Up to 20% OFF across the store!
Adafruit Diffused White 10mm LED (25 pack)
Deliveryfrom just £2.50
No Quibblereturns policy
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Next Day DeliveryOrder by 2PM*
Share this Product
Need some really bright and big indicator LEDs? We are big fans of these 10mm diffused white LEDs. They go easily into a breadboard and will add that extra zing to your project.
- Pack of 25 diffused white LEDs
- 10mm diameter
- Cool White color
- 3.0-3.4V Forward Voltage, at 20mA current
- 1,000 mcd brightness
- Viewing Angle : ±57 degrees
- Maximum continuous current: 20 mA
- Datasheet
If you need some help using LEDs, please read our "Introduction to using LEDs" tutorial for any electronics project.


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Bible Passage: Ephesians 6:10-17
Review it:
Stuff Phil said:
This passage relates to the other things we have talked about in this series.
· Impact of our Words
· The way we relate to each other
· How we keep from having a hint of the bad stuff in our lives
He talked about Jesus becoming bigger and bigger in our lives and us becoming less. The smaller Jesus is the more temptation you will face!
Your enemy is never another person!
To put on the armor of God...
· Engage daily with God's truth
· Give selflessly to others
· Learn from others further along
Following Jesus is a long obedience in the same direction.
Open it:
Phil quoted GK Chesterton, " The Christian ideal has not been tried and found wanting. It has been found difficult; and left untried." What do you think about that quote?
Explore it:
Read Ephesians 6:10-17
What are some of the right and wrong things people think about the devil? (If it gets crazy suggest people identify verses that back up what they are saying)
· People tend to maximize Satan and demons by seeing them behind everything that happens in the world, by thinking he is co -equal with God (remember he was created - an angel - kicked out of heaven) OR
· People tend to minimize Satan and demons by not thinking they are real, or ignoring, disregarding or not wanting to talk about it.
· The Bible says Satan is a liar, thief, accuser, destroyer.
What areas of our life would he likely target?
· How we think about ourselves, God and others, what we dwell on, our routines, families, finances, fears, church, community etc...
· We need to recognize his fingerprints. Creating confusions, chaos, defeat, destruction, etc…
How does it help to know who our struggle is with and who it is not with? ( Eph 6:12 )
What are some things that Phil shared and that you can see in this passage that will help us to stand firm against the schemes of the devil?
· More Jesus less me.
· Start
· Grow
· There are things that we must do in the battle and ways to be prepared.
How do we grow?
· Word of God, wise people, serve selflessly, etc…
How do these help us to have more Jesus and less me?
Painful times, difficult seasons, and temptations will come. Although some would tell us everything is better when you give your life to following Christ the Bible tells the truth that in this world you will have trouble, temptations, and difficult time. From this passage in Ephesians and others you may think of, what should we remember and what should we do when difficult times do come?
This passage uses the picture of a soldier prepared for battle and how he would be prepared and dressed. What are mentioned in these verses as important to the battle?
· Truth, Righteousness, Faith, Word, Spirit, Peace
How can you use these things for the battles you are facing now or in the future?
Back to the beginning: How does this battle that we are engaged in relate to the impact of our words, the way we relate to each other and the temptations in our lives?
Apply It:
What can you do this week to armor up?


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Cow Pies
Yield: 2 dozen
2 c (12 ounces) milk chocolate
- chips
1 T Shortening 1/2 c Raisins 1/2 c Chopped slivered almonds
In a double boiler over simmering water, melt the chocolate chips and shortening, stirring until smooth. Remove from the heat; stir in raisins and almonds. Drop by tablespoonfuls onto waxed paper. Chill until ready to serve.


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Is kokm right for me?
Kokm can help businesses of all sizes work smarter and move faster.
Kokm is great for an organisation wanting to have one portal or place to work, learn, share and even sell!
It can be your whole business website with a great design or a place for your people and extended enterprise to work together.


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Advance Ticket Sale – PSA #7April 15, 2017
ADVANCE TICKET SALE codes have been sent via mailchimp. Please check all your folders. You will find two emails, the one…Read More
Greeter and Volunteer Shifts for Theme CampsApril 14, 2017
A huge thank you to theme camps- your participation has made Greeter shifts a tremendous success! Based on this success and…Read More
BitF Volunteer Signup is OPEN!April 9, 2017
Ready… Set… GO!!!! Sign up for crew shifts at BitF 2017! Read more about it on the Be The Crew page,…Read More
PSA #6 Directed Tickets for Volunteers and Production folksApril 9, 2017
***NOTE: This is for Directed Tickets for Production and folks who already have a key BitF 2017 role. Emails and instructions…Read More
PSA #5 Advance tickets for Volunteers; Directed Ticket emailsApril 8, 2017
Advanced Tickets for Volunteers From Laura and Shayla (BitF 2017 Volunteer Group Production): Hi everyone, If you've been receiving emails about the…Read More
LOW-INCOME TICKET APPLICATION ARE OPEN; GENERAL TICKET REGISTRATION OPENS APRIL 8April 6, 2017
LOW-INCOME TICKET APPLICATION ARE OPEN Apply for a Low-income ticket at: http://burnintheforest.com/subsidy/(Closes on April 15th @ 11:59:59 PM PDT) GENERAL TICKET REGISTRATION…Read More


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Senior PHP Developer
- Recruiter
- Saffa Associates
- Location
- Nottingham
- Salary
- £35-45,000pa + bonus
- Posted
- 29 Jul 2017
- Expires
- 14 Aug 2017
- Sector
- Information Technology
We are currently recruiting for an experienced Senior PHP Developer for an established business in Nottingham. You will be joining an established team of Developers and have a Junior Developer reporting directly to you, who you will train, mentor and develop. You will be responsible for managing projects and agreeing solutions yourself, so project management experience is ideal. You will be focused on removing pain points and working on tasks that make a genuine difference to the business and end users. The successful candidate will have experience of: - PHP with particular attention on Object Orientated Programming - MVC frameworks, ideally Symfony or Laravel - Experience working with Agile methodologies - HTML5, CSS3, JavaScript and jQuery - Version control - Git - Testing - ie PHPUnit or Behat - Experience working with a third party API Desirable Skills - Bootstrap, Gulp, NodeJS and Bower - Serverless technologies - AWS experience This company offer flexible working, with the ability to work from home, career development, childcare vouchers, annual bonus pool, regular team events and a dog-friendly office so if this sounds interesting, please apply immediately for a confidential discussion.


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Here comes the third installment of the teaser in a technical translation series from HTC which pertains to the all new HTC One. The latest teaser sheds some light on the device's ultrapixel camera with the information in the video shared by a tech geek which the other guy in the video translates to an easy-to-understand language.
According to the engineer in the teaser, there is a 2.0 micrometer pixel that HTC has used in this version of the HTC One smartphone and also explains how the ultrapixels allow 300% more light into the camera sensor which means that the quality is maintained even while shooting under low light conditions.
Recommended: Top 5 Nokia Smartphones That Make Android Fans Jealous
Sadly, since it is a teaser, when the engineer and the other guy start explaining about the new HTC One, the dialogue on the video is beeped out and the device itself is also blurred to keep it a suspense. However, as we have already seen in the leaked images earlier, the new model is expected to feature a dual-sensor in a rear-facing cameras in the back.
Recommended: Top 10 latest 5 Inch Android Smartphones With 3G Support Under Rs 10,000
Combining the two sensors, it is expected to bring better depth perception to pictures taken with the phone and allow better photographs even under low-light conditions. While at the front, the smartphone is expected to bear a 5MP camera with an f/1.8 aperture to allow for sharp selfies even when the lighting conditions are challenging.

Stay tuned to GizBot!


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Van Nuys Vacation Packages
Many of Los Angeles's highlights are within easy reach of Van Nuys, making it a smart choice as a place to base yourself during your visit. Two attractions that should be on every visitor's itinerary is the fascinating Iceland Ice Skating Rink and the ever-popular Van Nuys Sherman Oaks War Memorial Park. Also, well worth the effort are the Sherman Oaks Castle Park and the Van Nuys Golf Course.
If the sun is out, follow the lead of the locals who know the area best and appreciate the nearby parks and reserves. There are some pleasant urban parks, so take a picnic blanket to the Anthony C. Beilenson Park in Encino.
To play golf in a new setting, hit the fairways such as the Woodley Lakes Golf Course.
Spend a few hours at the Sherman Oaks Galleria in Sherman Oaks for a satisfying dose of retail therapy.
After spending the day discovering the sights, sounds and smells of Van Nuys, put up your feet and relax in one of the area's popular bed-and-breakfasts, hotels or short-stay apartments. For a good night's sleep check out the Best Western Plus Carriage Inn, Sherman Oaks, the Airtel Plaza Hotel & Conference Center, and the popular Mikado Hotel, North Hollywood.


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Butterick 3451; ca. 1965; Two-Piece Dress. Back buttoned, three-quarter length sleeved overblouse with large Peter Pan collar, contrasting ribbon bow. (A) Ribbon band on hem of overblouse. (B) Contrast collar.
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Add a photo to the gallery by clicking the "modify" button below.
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Insight, wit and analysis as BBC correspondents, journalists and writers take a closer look at the stories behind the headlines. Presented by Kate Adie and Pascale Harter.
February 23rd, 2013
Episode 350 of 867 episodes
When Madeleine Morris returned to her native Australia after twelve years in the UK she knew she'd find things different there, but she didn't realise her wallet would take such a battering! Ian Pannell, in northern Syria, finds a group of little boys sheltering in a Roman-era tomb and asking where their mother's got to. Craig Jeffrey's at the Indian college where once students demonstrated for the right to cheat in exams. Sicily looks like being one of the key battlegrounds in the Italian election -Chris Morris is there communing with the dead ahead of the big vote. And as France prepared to take on England in the Six Nations tournament at Twickenham, Chris Bockman finds the congregation deep in prayer at a church they call 'Our Lady of Rugby.'
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Welcome to the Brain Training Podcast, the daily audio workout for your head. In this podcast we have two games for you, each with three rounds which get progressively harder. To enjoy the full experience, relax, and avoid distractions whilst you listen.