text:
Grateful Dead
"I first met Rolling Thunder, a medicine man who lives
in Nevada, in 1970 and have since observed him conduct
several healing sessions and consultations. Once I
listened to him give instructions to a client who had come
for dream interpretation. Instead of telling the client what
the dream meant, Rolling Thunder asked the client to imagine
himself as each of the characters in the dream so that he
would be able to interpret the dream himself. He gave him
the tools rather than the answers."
-Stanley Krippner, Ph.D.
"Creativity requires the courage
to let go of certainties."
-Erich Fromm
"Any activity becomes creative when
the doer cares about doing it right,
or doing it better."
-John Updike
"Grief reunites you with what you've lost. It's a merging; you go with the loved thing or person that's going away. You follow it a far as you can go.
But finally,the grief goes away and you phase back into the world. Without him.
And you can accept that. What the hell choice is there? You cry, you continue to cry, because you don't ever completely come back from where you went with him -- a fragment broken off your pulsing, pumping heart is there still. A cut that never heals.
And if, when it happens to you over and over again in life, too much of your heart does finally go away, then you can't feel grief any more. And then you yourself are ready to die. You'll walk up the inclined ladder and someone else will remain behind grieving for you."
― Philip K. Dick, Flow My Tears, the Policeman Said
"Barefoot conducts his seminars on his houseboat in Sausalito. It costs a hundred dollars to find out why we are on this Earth. You also get a sandwich, but I wasn't hungry that day. John Lennon had just been killed and I think I know why we are on this Earth; it's to find out that what you love the most will be taken away from you, probably due to an error in high places rather than by design."
― Philip K. Dick
"Judge a man by his
questions rather than
by his answers."
In speaking about creativity,
E. L. Doctorow said...
"It's like driving a car at night.
You never see further than your
headlights, but you can make the
whole trip that way."
"May Father Sky light all your trails,
And may Earth Mother keep them
-Mary Summer Rain
"The State thus becomes an instrument by which the power of the whole herd is wielded for the benefit of a class. The rulers soon learn to capitalize the reverence which the State produces in the majority, and turn it into a general resistance toward a lessening of their privileges. The sanctity of the State becomes identified with the sanctity of the ruling class..."
Thanks for the link, Mike Edwards


text:
Thursday night, January 22nd 2015, after attending the Mountain Grove PRC meeting, 69 year old rancher Bob Estep was heading to his home near Sparta when a car with it's brights on pulled in behind him. Estep said he was trying to let the driver behind him know that the brights were blinding him by moving his rearview mirror attempting to flash the lights to the driver to his rear and moving the mirror around caused Estep to weave a little bit as he traveled down the road.
According to Estep, approximately 3 miles outside Sparta as he neared his turn off point, the vehicle behind Bob turned on it's emergency lights indicating that it was a patrol car. Estep pulled over and waited for the officer to come to the side of his car. According to Bob Estep, he was waiting a rather lengthy amount of time, and he became concerned that there may be a problem, so he called 911.
When the 911 operator answered Bob began to explain the situation and then his door was suddenly ripped open and he was tased without any verbal instructions or explanation. The power of the taser threw him into the back seat of the car. Estep says he was then instructed to get out of the car, but he had been paralyzed by the taser and wasn't able to comply and the officer threatened to tase him again if he didn't get out.
Then Estep was pulled out of the car impacting his face on the pavement, and his feet became stuck under the car in the process. The officer instructed Estep to roll over and since his feet were stuck and he was still somewhat immobilized by the taser he couldn't comply. Estep says the officer then stomped on his back four times and then pulled him from under the car.
Mr. Estep was arrested by Officer Spencer of the Sparta Police Department for driving while intoxicated and resisting arrest, and cited for weaving and failing to comply with the lawful orders of a law enforcement officer.
Apparently all of this was caught on the 911 call and we are in the process of trying to get that recording to share with you. Bob Estep was given a breathalyzer test and found to have no alcohol level indicated as he had only consumed lemonade and water that evening.
As of this writing, Mr Estep is in the hospital suffering from nerve trauma and heart issues related to the tasing.
As more information and documentation becomes available, we will make it available to our readers.

subset: commoncrawl


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Hotel Corona3
Via Napoli , 3 Roma, Roma, 00184
Price FromView Rooms
- Excellent
- 1 guest reviews
"For family and beyond, is the best place to spent time in Rome. Everything in the city center is between 10 to 30 min walk. No need to use p..."
About the hotel
A short walk away from Repubblica Metro Station and Termini Railway Station, this simple 3-star hotel offers a free breakfast buffet, helpful multilingual staff and a central location in Rome. Just around the corner from Rome's Opera House, Hotel Corona is set in an old building on a quiet side street. A small market is close by and you will be within walking distance of main monuments.
This no-frills hotel provides an on-site restaurant, an air-conditioned breakfast room, and small lounges. Wi-Fi access is available in the lobby at an additional cost. Hotel Corona has small clean rooms with en suite bathrooms, direct-dial telephones, a safe, a hairdryer, and satellite TV. Friendly staff can arrange city tours and book museum tickets. Ask about scooter, bicycle and car rentals. Air conditioning is centralised and controlled by the front desk.
Read MoreLess
Facilities Show/hide
- Main
- Hotel Non-Smoking Throughout, Air conditioning in rooms, 24 Hour Reception, Cots available, Safety Deposit Box, Hairdryer in each room, Telephone In Each Room, Limited Hours Room Service, Concierge, Wake Up Call Available, Currency Exchange, Interconnecting rooms available, Pets Allowed, Doctor on call, Lift to all floors, Public Air Conditioning
- Parking
- Airport Shuttle, Car Parking Available Offsite
- Business
- Wi-Fi Available, In-room Broadband, Wi-Fi Available In Public Areas, Wi-Fi Available In Rooms
- Dining
- Restaurant
- In Room Entertainment
- TV, Satellite TV, Radio
- Leisure
- Mountain Bike Hire, [Channels] News, [Channels] Foreign (European), [Channels] Foreign (Other), [Channels] Music, [Channels] Childrens
Can't find the facilities you're looking for?
- Call us on 0333 300 1007 and we'll help find the perfect hotel for you
- Call
Useful Info Show/hide
- From 13:00 hours
- By 10:00 hours
- Up to 8 years old
- The LateRooms.com rate for Hotel Corona in Roma is the total price of the room and not the 'per person' rate. All taxes are included, with the exception of city tax, which will be charged at 4.00(EUR) per person per night and is payable to the hotel directly.
- Pets are allowed
Customer Reviews
Our reviews are from genuine LateRooms.com customers. We verify that the customer has stayed before leaving a review.
100% would recommend it to a friend
Customer service
Food quality
Room quality
Excellent place to stay in Rome!
For family and beyond, is the best place to spent time in Rome.
Everything in the city center is between 10 to 30 min walk. No need to use p...
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text:
Log Into Your Account
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Enter new zip code to refresh estimated delivery time.
PayPal Orders
Payments processed using PayPal will be charged immediately (as opposed to when an order ships).
You Pay:
The Profoto 254582 Bag is a soft-padded bag that fits one Giant Reflector 180 or Giant 210.


text:
Here are some links to 2016 Summer Camps.
UTRGV High School Research Scholars Program (HIGH
Information Packet
The College of Sciences at UTRGV has established an
exciting new program to engage high school
students from the Rio Grande Valley in research projects being
conducted by the university faculty at
UTRGV's Brownsville and Edinburg campuses. The goal of this
10-week summer research program
is to promote student interest in Science, Technology,
Engineering, and Mathematics (STEM) by
engaging students in "real time" laboratory or field research
Copyright © 2017 Weslaco High School


text:
Most Helpful Guy
This is yet another case of a non-Westerner complaining that no one in the West is paying attention to what's happening in Syria and other places while at the same time, we immediately start talking massively about whenever something happens in Europe.
The typical claim is that Europeans and Americans are apathetic towards the Middle East or Africa. But that's bullshit. Think of it this way - what happened in Brussels is closer to home. It's in Europe. Belgium is a country with a culture similar to many other European and North American cultures. Westerner care because Belgium is IN the West.
If a house burns down in a neighboring city - you won't even know. You won't even care. But if the house next door catches fire, you damn well will care.
So yes, I will metaphorically pray (I'm not religious) for Belgium while not doing so for Syria. Why? To put it bluntly and somewhat offensively - I don't care about Syria. Syria wouldn't care if Latvia had a civil war too. But Belgium is a place I can relate to some small extent. So I care about it.00|00|0
Most Helpful Girl
Pray for who ever you want. Most people don't even pray at all.00|00|0


text:
Digication ePortfolio Student Tutortial MP3
http://my.brainshark.com/Digication-ePortfolio-Student-Tutortial-289730674 - The following training tutorial is intended to train students of Kingsborough ...
Professional Portfolio: Self-Introduction MP3
https://bu.digication.com/wccf/ I'm in the processing of developing a professional portfolio that captures the range of skills and experience I bring to the TESOL ...
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Scholarly sources - https://bu.digication.com/gregoryjameswilson/Black_Ideologies_as_seen_in_Invisible_Man The Invisible Man by Ralph Ellison Booker T.
Tips for BU's Newest International Students: Chrissie Chinebuah MP3
Some of Boston University's international student upperclassmen offer suggestions for new students arriving from abroad on how best to transition to life at BU.
ePortfolio Story MP3
How ePortfolios are used by students at Boston University.
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The brothers of Sigma Phi Epsilon welcome you to spectate a campus-wide Rhett's Challenge. Two dozen competitors from other student groups will face off in ...
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Help us caption & translate this video! http://amara.org/v/FKVh/
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Boise Youth Spectacular (BYS) is a 3 day youth Conference held at BSU and the Boise LDS Institute for teenagers age 14-17. July 18-20 & 21-23, two sessions ...
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Sanatçı: Şakir Gökçebağ Küratör: Marcus Graf Kamera- Kurgu: Arda Öztürk Açılış: 03.04.2013 Sergi Süresi: 03.04.2013 - 12.05.2013 Her gün 10.00-20.00 arası ...
Submit your ePortfolio MP3
by sharing it with user "Personnel Portfolios" with permission to copy.
ePortfolio 4 make a copy for your instructor MP3
August 2013. Part of a video series on how to make an ePortfolio using Google Sites made for the University of Arkansas at Little Rock's Department of Rhetoric ...


text:
Nancy is of Caucasian descent.
Nancy began skating at the age of six.
In 1994, Nancy did print ads for Reebok's Planet Reebok cross training shoe campaign.
Nancy was named one of People Magazine's Most Beautiful People of 1993.
Nancy's mother is legally blind.
In 1992, Nancy did a television commercial for Reebok.
In August 2000, Nancy's dad Dan won $1,000,000 on a $5 scratch-off lottery ticket at his local Massachusetts beverage store.
According to Celebrity Sleuth magazine, Nancy's measurements are 32A/B-24-35.
Nancy stands at 5'4" or 1.63 m.
Nancy won gold at the 1990/91 Nations Cup.
Nancy scored 5th place at the 1989/90 Goodwill Games.


text:
This is a disambiguation page; that is, one that points to other pages that might otherwise have the same name. If you followed a link here, you might want to go back and fix that link to point to the appropriate specific page.
Tribune can refer to several things. Did you mean:
- Tribune, an internal rank within the Ventrue in Vampire: The Masquerade?
- Tribune, an internal rank within the Order of Hermes in Mage: The Ascension?


text:
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text:
Inbox/Outbox: June 20, 2014
Last week, I celebrate the official arrival of summer by spending five days at the beach. Like always, I planned for TONS of reading time and took a stack of books. And like almost-always, I spent way more time frolicking in the surf and hanging with family than I did turning pages. But! I return to you rested, reasonably read-up, and dusted with freckles. Let's do this!
Inbox (Books Acquired)
Stone Mattress: Nine Tales by Margaret Atwood (Nan A. Talese, September 16)–My first experience with Margaret Atwood was with her 2006 short story collection Moral Disorder. It rocked my world and set me off on a love affair with her novels. The jacket copy promises "acute insight, turbulent relationships, and psychological aberration," and I couldn't be more excited to read these stories. Atwood is a master of storytelling in myriad formats and structures, but there's something extra-magical about her ability to compress action and meaning into short-form. This is one of my most anticipated books of 2014.
Father of the Rain by Lily King–I devoured King's new novel Euphoria last week–it's a fantastic story about three anthropologists in New Guinea in the 1930s who find themselves embroiled in professional competition and a love triangle of sorts–so when this book from her backlist showed up on sale this week, I couldn't pass it up. WASPs! Family dynamics! Politics! Can't wait.
Outbox (Books Finished)
Glory O'Brien's History of the Future by A.S. King (Little, Brown Books for Young Readers, October 14)–Only A.S. King would explore a possible future in which the end of women's rights results in a second American civil war using a story about two teenage girls who mix a petrified bat with beer, drink it, and find themselves able to see other people's infinite pasts and futures. And that's a good thing, because only A.S. King could pull it off. This is a wonderfully weird yet delightfully grounded story about feminism, identity, friendship, and figuring out not just what you want to be when you grow up, but how you want to be. King is wicked smart, and she trusts her readers to pick up what she's throwing down, and what she's throwing down is a hell of a lot of wisdom wrapped up in a book that's a blast to read.
Lumberjanes #3 by Grace Ellis and Noelle Stevenson, art by Brooke A. Allen–What's not to love about a fun, quirky, smart comic about a group of girls hanging out at a summer camp for "Hardcore Ladytypes?" Nothing. Nothing is not to love. This new series is getting a lot of buzz in the comicsphere, and for very good reason. And if you're new to comics (or you want to be), it'd be a great starting point.
In the Queue
Americanah by Chimamanda Ngozi Adichie–This isn't so much in the queue as it is in process. I finally started Americanah last week, and I was sucked in from the get-go. Adichie's writing is intelligent and funny, her cultural criticism pointed but never preachy. I'm about halfway through, and it's everything I can do not to blow off work and ignore all of my responsibilities until I finish.
That's it for me, pals. What have you been reading lately?


text:
Mp3 Tracklists
0 0
Chidozie Okoro aka "Mc Chido" drops the audio for his new single "Skele" Produced by Dj Coublon. The Ebonyi State, Nigeria native based in the USA, is ready to storm the world with his versatile music style. The visuals to this sweet melody is currently being shot in locations across the US and it will […]
- 1


text:
And we couldn't tear our eyes away
Blimey, that was a pretty amazing BRITs evening, wasn't it? The glitz, the awards, the Taylor Swift reaction to Kanye West's performance, the – oh hold on. No. Forget EVERYTHING else that just happened, because MADONNA FELL OFF THE STAGE DURING HER LIVE PERFORMANCE.
The shocking moment happened right at the end of the evening when one of her dancers tugged a little too enthusiastically on her cape, and she was dragged right off the stage and down a flight of stairs.
The BEST moments from the entire show - catch up on everything you missed
ALL the best Vines of the night - Tay's reaction face is priceless!
Celebrity Instagrams - how the stars got ready for the Brit Awards
Ed Sheeran tells us all about his and Taylor's Pizza Express date!
Poor Madge. We caught the whole thing on video just so you can see exactly what happened.
Luckily she let us know on Instagram that she was OK afterwards. Phew.
"Armani hooked me up! My beautiful cape was tied too tight! But nothing can stop me and love really lifted me up! Thanks for your good wishes! I'm fine! ❤#livingforlove."
Sheesh. Hope you're OK, Your Madgesty.


text:
Readerpix: Drunks not included 2.0
Hoboken411 reader Matt found out he and some friends were in a picture used here for other purposes last week – and I'm relieved he was a good sport about it!
Hoboken411 reader photo of the day – 3/16/2009
"On Hoboken St. Patrick's Day, a picture was taken outside of Hoboken High School, and used for a Hoboken411 story on Senior Prep Day. And in that picture, 3 wandering revelers were captured, with a rather funny comment made in the photo: "Drunks not included" (hold mouse over to see pic), with an arrow pointing to the group. I am happy to say that I am one of those random passers-by, along with my friends Tim and Lou. Now this photo has become something of a legend in my circle of friends (many of whom are avid Hoboken411.com readers, which is how we discovered this happy coincidence in the first place).
Anyway, back to my photo submission. This past Saturday (March 14), some friends and I, prompted by nothing more than boredom, decided to stage an impromptu Hoboken bar crawl, stopping only at places off the beaten path (Carpe Diem, DC's, Mario's, etc.) During our travels, we found ourselves walking past Hoboken High and the very sign we were randomly photographed in front of a week earlier. So, using only our memory, we tried our best to recreate the photo, with a few difference. We are not wearing green, and we substituted Lou (the short guy in the back wearing the funny hat in the original picture) with our friend Ryan, because Lou wasn't with us during the bar crawl. So, here it is… our humble attempt to recreate what we thought to be a very funny picture!"
[Stay tuned for tomorrow's Readerpix entry! – Note: one reader's photo(s) will be featured each day at approximately 11am. Unless your photo is time-sensitive – it will be published in the order it was received! Additional note: Your pictures will have a better chance of being published if they're in "landscape" mode, as opposed to the taller "portrait" modes… ]


text:
We know each other through a friend and have yet to meet in person (although I want to asap!)
So far, we've texted three days in a row (he initiated the 1st, I the 2nd and he the 3rd), nothing yesterday and today would be the 5th day.
Should I give it another day's break? Or talk to him as I am longing to do? I don't want to come off as clingy or annoying.
How often should a girl text a guy? Is it okay for her to often initiate the convo?
Most Helpful Guy
Its hard to say how often cuz it depends on the guy. Personally I HATE those stupid games. I prefer honesty. If you want to talk to me, then text me. If I'm into a girl I don't mind at all. It doesn't matter to me at all who initiates the convo.00|00|0
I love that. I feel the same way, I'm a very honest and open person but at the same time, I'm always trying to respect and understand the way that others see things as well. I guess that that's where all of this self doubt is coming from!
Thats a great personality trait, trying to respect others ideas/feelings. I would say at some point just talk to him about how you are and why. He will understand. If it was me, not only would I understand but I would be flattered. If he doesn't at least understand those things about you then he's not worthy of you, period lol.


text:
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SALT LAKE CITY — Before heading up here, Phil Jackson was asked about getting fined by the NBA for criticizing referee performance at Utah Jazz home games, and he smiled and said: "I have amnesia about that situation."
Perhaps that moment in the final minutes of the game Friday night when Jackson saw Carlos Boozer shove Derek Fisher aside, accept the ball and hit a jumper gave Jackson more clarity of mind. Or maybe it was Jackson practically running after referee Monty McCutchen on the sideline after that play. (I say "practically," because Jackson's can't really run anymore, even though he's wearing those sneaker-style dress shoes again with his suits because his hips are hurting.)
Anyway, here's what Jackson said today when told Utah coach Jerry Sloan wasn't happy with some things Kobe Bryant might be getting away with: "He (Sloan) can't even open his mouth to speak about that the way Boozer pushes and shoves out there. I mean, he got away with all his pushes (Friday night) that he was getting called on in L.A., so in that regard you just let it go and say the stars get to play their games and the other guys have to keep their hands to themselves."


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|« Previous month
|Next month »
One of America's biggest poultry firms has secured exports to Iraq – thanks to help from China.
Thai broiler operator CP Foods has set out to raise industry standards with the implementation of the Good Labour Policy (GLP) for Poultry Farm and Hatchery in Thailand.
The South African poultry sector is in crisis, according to RCL Foods which has posted extensive losses for its poultry division over the past 12 months.
With the aim of raising their meat processing and packaging capacities, two Czech meat processors have unveiled investments of a total of CZK90 million (€3.4 million).
Meat production in Malta dropped 3.8% in the first half of 2016, compared to the same period in 2015, figures from the National Statistics Office have revealed.
The United States Department of Agriculture (USDA) has set out an ambitious forecast for US beef exports over the next 12 months.
The purchase of a Uruguayan beef processor by a giant Chinese industrial conglomerate looks well-timed as China dangles the prospect of a free trade deal before the Latin American nation. ...
EU farming body Copa-Cogeca has warned that pork and beef markets in Europe are struggling a week after the European Commission delivered a £500 million aid package to farmers.
Bangladesh is mulling controls over foreign investors in the country's growing poultry industry after local rivals said they fear being swallowed by overseas companies.
Meat processor Green Bay Dressed Beef, operating as American Foods Group, has issued a recall on 7,420 pounds (3.7 tonnes) of beef brains.
China's ever-growing appetite for international pork helped prevent a greater drop in Canada's exports of fresh and frozen pig meat in the first six months of the year. ...
Investigations into the health of pigs exported from Jiangxi are underway after Chinese authorities blocked trade following a warning from Hong Kong's Food and Environmental Hygiene Department (FEHD).
An outbreak of Q fever at a meat-processing plant in Melbourne, Australia has put regional health officials on high alert.
The number of participating nations in this year's World Steak Challenge has increased by 60% since the inaugural event kick-off at London's Hyde Park in September 2015.
Latvian poultry processor Kekava Poultry Farm plans to invest €2.5m to modernise its poultry breeding facility and raise export sales, according to chief executive Anris Pranckevicius.
Ukraine has lost $1bn-worth of trade thanks to the Russian-inflicted food embargo, according to Natalia Mykolska Ukraine's Deputy Minister of Economic and Trade Development.
The government of India's semi-autonomous Punjab region is planning to build the country's most advanced pig-processing facility as India's consumption of pork and reliance on meat imports both continue to...
US white-feather chicken meat imported into China will face "anti-subsidy duties" for five more years, China's ministry of commerce has announced.
Australian government and meat industry agencies are boosting their efforts to increase biosecurity for the country's critically important livestock sector.
Fast-food giant KFC has launched a limited edition range of chicken-scented sunscreen to get you smelling better than the iconic moustachioed colonel himself.
Undercover investigations by a string of animal rights groups has detailed how Irish calves have been exported to France in "horrific" conditions that breach European law.
Yonekyu Corporation, one of Japan's leading processed meat companies, has applauded the impact of Sealpac's thermoformers on its production lines, claiming the equipment is "extremely strong".
US company Pinnacle Foods is to cut 85 manufacturing jobs as it moves production of its growing frozen meals brand Evol Foods from Colorado to Arkansas.
Cattle Council of Australia (CCA) CEO Jed Matz has welcomed a report from the Australian Meat Processor Corporation, which assessed the dangers of tighter competition laws.
Two government departments in Thailand alongside the Thai Broilers Processing Exporters Association (TBA) will improve conditions in the chicken industry, following claims of modern-day slavery.
|« Previous month
|Next month »


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Ok so I have a bride that wants a cosada(sp) cake for her rehersal dinner.....problem is I have never heard of it. She said its like an Italian Wedding cake. Have any of you heard of this kind of cake??
i googled it, and it's spelled casada. try googling it and you will see several sites with recipes. Ive never heard of it before today =)
I tried to google it and apparently there are THREE different ways to make the cake! My customer is from Ohio and says its very popular there.....
i would probably contact the customer and ask her which preparation she is wanting. there's no harm in that =)
around here we say Cassata and it's a yellow cake layered with strawberries and sprinkled with almonds...
I am from Ohio...it is a white or yellow cake with a layer of custard and a layer of strawberry filling iced in whipped cream. I am doing one for tomorrow but using my cheesecake filling:
Sorry guys....could not find it. This has to be refrigerated as well as any cake you put it in. Makes about 3 cups cake filling and keeps 2-3 days in fridge. Can be made ahead and frozen (that and rebeat before using).
1 small Pkg Jello Instant French Vanilla Pudding
1 Envelope Dream Whip
1-1/2 cups light cream
4 oz Philadelphia Cream Cheese softened
2 tsp pure vanilla extract
1 tsp Knox unflavored gelatin
1/4 cup cold water
Pour powdered pudding and powdered Dream whip into medium bowl and add the light cream, blend on low speed with electric mixture until blended. Switch to high speed and beat 6 minutes scraping down sides occasionally with spatula. Add softened cream cheese and vanilla and beat another minute. Pour 1/4 cup water into small saucepan and sprinkle gelatin over it. Stir and the let stand for one minute. Heat gelatin mixture over medium heat until gelatin dissolves and mixture thins ad turns clear. DO NOT allow to boil. Remove pan from heat and set aside for a few minutes and allow mixture to cool off a bit. Don't let sit too long-it will begin to gel after about 10 mins. or so. Pour gelatin mixture into pudding mixture and beat thoroughly, scraping down sides of bowl for 1 minute. Cover tightly and refrigerate for at least 3 hours. When ready to fill cake mix with mixer until nice and creamy. If too thick add a little bit light cream until desired texture achieved. TIPS: recipe can be doubled-use a large bowl. Flavors develop when sitting over night. If cake you are filling is crumbly, pipe filling between layers and spread with knife dipped in hot water. (Recipe courtesy of Carol Chisholm.) Delicious, creamy, not too sweet and sturdy enough now to squish out from between layers. ENJOY!!!!!! Pat in OHIO (I use the strawberry filling by the "CakeLady" on www.ladycakes.com. If yu need help with whipped icing ask me...[email protected]
I would definately call her back and ask her what she had in mind. Tell her you have several different versions of the cake.
Here are a few ideas:
2 lbs. fine Ricotta cheese
28 eggs
2 c. sugar
2 tsp. cinnamon
1 box citron
1 (8 oz.) bottle maraschino cherries, chopped and drained
1 (13 oz.) Hershey bar, grated
Combine with electric mixer eggs and sugar until sugar is dissolved. Add Ricotta; mix thoroughly. Add Hershey; mix again. Pour into greased springform pans (2-8 inch or 1 large pan). Drop in citron and cherries; sprinkle cinnamon on top. It will make its own crust. Bake at 350 degrees for 2-2 1/2 hours. Check with tester until not wet in middle.
1 box yellow cake mix
1 sm. box instant vanilla pudding
1 sm. Cool Whip
1 (8 oz.) cream cheese
1/2 c. chopped nuts
2 lg. cans crushed pineapple, drained
Mix cake as directed on box. Mix pudding while cake is baking and refrigerate. Soften cream cheese and beat with mixer. Mix pudding and cream cheese together, put on top of cooled cake. Put on drained pineapple, then put on Cool Whip and top with nuts.
1 pkg. yellow cake mix
2 lbs. Ricotta cheese
1 c. sugar
1 tsp. almond extract
1/2 to 1 c. miniature chocolate chips
1/2 to 1 c. chopped filberts
1/4 c. each red and green candied cherries
2 c. whipped cream, whipped with 1/4 c. powdered sugar
Prepare cake according to package directions, baking in bundt pan. Remove cake from pan. When cake is completely cool, slice into 3 layers.
For Filling: Combine Ricotta cheese, sugar, almond extract, miniature chocolate chips, chopped filberts, and candied cherries. Spread mixture between layers. Frost cake with whipped cream, whipped with 1/4 cup powdered sugar.
You guys are awesome! Thanks so much for the help!


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text: 23 April 2007 Letter of the week: Global dogfights coming to France Sign up for our weekly email * Agnès Poirier's article "New Huguenots with a love of danger" (France Special, 9 April) seems yet another shot in a systematic denigration of France that seeks to turn the country into the new sick man of Europe. Its thesis is that young French people are "protected and looked after from an early age by the mother state", which explains the exodus of so many of them to London, where they are free to exploit each other. Tell that to the underprivileged youth of the banlieues and see what reaction you get. The fact is that only a very small number of young French people have the privilege of the protection she cites. It's not so much a question of "new Huguenots" as one of a return to 19th-century slash-and-burn capitalism. In any case, if this is what they want, they have only to vote for either of the two main presidential candidates, as both Nicolas Sarkozy's and Ségolène Royal's projected changes to the French system of social protection will clear the way for the dogfights Poirier believes they want. Jeffry Kaplow London SE3 Subscribe from just £1 per issue More


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A reduction in fatty acid oxidation has been associated with lipid accumulation and insulin resistance in the skeletal muscle of obese individuals. We examined whether this decrease in fatty acid oxidation was attributable to a reduction in muscle mitochondrial content and/or a dysfunction in fatty acid oxidation within mitochondria obtained from skeletal muscle of age-matched, lean [body mass index (BMI) = 23.3 ± 0.7 kg/m2] and obese women (BMI = 37.6 ± 2.2 kg/m2). The mitochondrial marker enzymes citrate synthase (−34%), β-hydroxyacyl-CoA dehydrogenase (−17%), and cytochrome c oxidase (−32%) were reduced (P < 0.05) in obese participants, indicating that mitochondrial content was diminished. Obesity did not alter the ability of isolated mitochondria to oxidize palmitate; however, fatty acid oxidation was reduced at the whole muscle level by 28% (P < 0.05) in the obese. Mitochondrial fatty acid translocase (FAT/CD36) did not differ in lean and obese individuals, but mitochondrial FAT/CD36 was correlated with mitochondrial fatty acid oxidation (r = 0.67, P < 0.05). We conclude that the reduction in fatty acid oxidation in obese individuals is attributable to a decrease in mitochondrial content, not to an intrinsic defect in the mitochondria obtained from skeletal muscle of obese individuals. In addition, it appears that mitochondrial FAT/CD36 may be involved in regulating fatty acid oxidation in human skeletal muscle.
- obesity
- mitochondria
- fatty acid translocase/CD36
- transport proteins
it has been demonstrated that obese individuals have increases in intramuscular triacylglycerol (IMTG) storage, and this has been associated with insulin resistance (14–16, 23, 34). However, it appears unlikely that increases in IMTG levels represent a dysfunction in lipid metabolism (for review, see Ref. 39), and more reactive lipids, namely, diacylglycerol (DAG) and ceramides, may be important for the development of insulin resistance (18, 29, 34). It has been suggested that reductions in fatty acid oxidation in obese individuals contribute to the intramuscular lipid accumulation (20, 22, 24, 25, 31, 38). Based on reductions in carnitine palmitoyltransferase I (CPT I) activity in obese skeletal muscle, it has been proposed that fatty acid transport into the mitochondria is diminished. This may account for the observed reduction in fatty acid oxidation (22, 25), although other proteins may also be involved in this process.
The transport of fatty acids across the plasma membrane is generally believed to be largely mediated by fatty acid transporters, among which fatty acid translocase (FAT/CD36) appears to have a prominent role. This protein is located in several subcellular domains (5), and recently FAT/CD36 has also been found on rat and human muscle mitochondria (4, 10, 19). At this subcellular location, FAT/CD36, along with CPT I, contributes to regulation of mitochondrial fatty acid oxidation in skeletal muscle at rest, since blocking mitochondrial FAT/CD36 has been shown to almost completely inhibit fatty acid oxidation (4, 10, 19). During exercise, mitochondrial fatty acid oxidation is upregulated and is accompanied by an increase in mitochondrial FAT/CD36 content (10, 19). The exercise-induced increase in fatty acid oxidation is also completely inhibited when FAT/CD36 is pharmacologically blocked (10, 19). In other work, an increase in mitochondrial FAT/CD36 has been associated with exercise-induced weight loss and an improvement in whole body fatty acid oxidation (32). Thus it appears that changes in mitochondrial FAT/CD36 are associated with changes in mitochondrial fatty acid oxidation.
Fatty acid binding protein (FABPpm) is another well-recognized plasma membrane fatty acid transport protein that has also been found on mitochondria (3, 37). However, the role of FABPpm with respect to mitochondrial fatty acid oxidation remains unknown.
The net accumulation of lipids in obese skeletal muscle may result from a combination of increased whole muscle fatty acid uptake and/or decreased whole muscle oxidation. Although the concept of impaired fatty acid oxidation as a mechanism to increase intramuscular lipid species has gained attention in recent years, the exact mechanism remains unknown. However, two plausible explanations exist: either mitochondrial content is decreased, and/or there is a dysfunction in fatty acid oxidation within mitochondria. Work in skeletal muscle has indicated a reduction in mitochondrial content with obesity, along with a concomitant decrease in fatty acid oxidation (24). Although ratios of electron transport chain capacity to mitochondrial DNA and mitochondrial size have been used to infer dysfunction (24, 31), recently the ability of mitochondria to oxidize fatty acids was directly measured in skeletal muscle from type 2 diabetics and, controversially, was shown to be increased, although the underlying mechanism remains unknown (1).
The focus of the present study was to examine whether the obesity-related decreases in skeletal muscle lipid oxidation are attributable to 1) a reduction in mitochondrial content and/or 2) an intrinsic defect in mitochondria, and also 3) whether there are reductions in the content of mitochondrial fatty acid transport proteins. We hypothesized that obesity would be associated with decreases in skeletal muscle mitochondrial content, as well as decreases in the ability of mitochondria to oxidize fatty acids. In addition, we speculated that impairments in mitochondrial oxidation would be associated with reductions in the content of mitochondrial FAT/CD36 and FABPpm.
The participants were nine lean [body mass index (BMI) < 27 kg/m2; mean: 23.3 ± 0.7 kg/m2] and nine obese (BMI > 30 kg/m2; mean: 37.6 ± 2.2 kg/m2) nondiabetic women (Table 1). Subjects were admitted to McMaster Health Sciences Centre for abdominal surgery and gave informed written consent before participating in the study. The University of Guelph and McMaster University Ethics Committees approved the experimental procedures. Before participation, individuals were screened and excluded from the study if any known diseases or medications, as well as if weight fluctuations in the 6 mo preceding surgery, were reported, similar to procedures previously reported (7, 8).
Following an overnight fast (12–18 h), general anesthesia was induced with a short-acting barbiturate and maintained as required by a fentanyl and rocuronium volatile anesthetic mixture. A venous blood sample was sampled directly into a 5-ml heparinized tube, inverted, and placed on ice for future processing. A portion of the rectus abdominus muscle was sampled (∼350 mg) and immediately placed in ice-cold oxygenated buffer (modified Krebs-Henseleit buffer containing 8 mM glucose) for transport to the laboratory.
Mitochondrial enzyme activities.
A portion of the muscle (∼10 mg) was immediately homogenized in 100 vol/wt of a 100 mM potassium phosphate buffer and used for the measurements of maximal β-hydroxyacyl-CoA dehydrogenase (β-HAD) and citrate synthase (CS) activities. Total muscle β-HAD activity was measured in Tris·HCl buffer (50 mM Tris·HCl, 2 mM EDTA, and 250 μM NADH, pH 7.0) and 0.04% Triton-X. The reaction was started by addition of 100 μM acetoacetyl-CoA, and absorbance was measured at 340 nm over a 2-min period (37°C) (2). The CS activity was assayed spectrophotometrically at 37°C by measuring the disappearance of NADH at 412 nm (2).
CS activity was also determined in the isolated mitochondrial preparations. Intact mitochondrial CS activity was determined by first assaying the extramitochondrial fraction in the suspension (1:20 dilution) and then assaying the total CS activity of the suspension (1:20 dilution) after lysing the mitochondria with 0.04% Triton X-100 and repeated freeze-thawing. The net difference provided a measure of the viability of the mitochondrial preparation, as well as a measure of the mitochondria recovered during the isolation procedure (compared with the total muscle CS activity) (4). The mitochondrial recovery and viability were comparable to those reported previously by our group (4, 19).
Isolation of mitochondria from skeletal muscle.
Differential centrifugation was used to obtain pure and intact mitochondria containing both intermyofibrillar (IMF) and subsarcolemmal (SS) fractions (10). All procedures were identical to those previously published by our group (4, 10, 19). Briefly, muscle (∼350 mg) was homogenized with a tight-fitting Teflon pestle. The homogenate was centrifuged at 800 g for 10 min to separate the SS and IMF mitochondria. The IMF mitochondria were treated with a protease (0.025 ml/g; Sigma, St. Louis, MO) for exactly 5 min to digest the myofibrils. Further centrifugation was used to remove the myofibrils and recombine the IMF with the SS mitochondria. The combined samples were centrifuged twice at 10,000 g for 10 min. The pellet was resuspended in 1 μl of buffer per milligram of tissue. Following the oxidation measurements, the remaining mitochondria were further purified using a Percoll gradient for Western blot analyses. Samples were centrifuged at 20,000 g for 1 h, and the mitochondrial layer was removed. The Percoll was removed from the sample by further centrifugation at 20,000 g for 5 h.
Mitochondrial palmitate oxidation.
Labeled CO2 production and acid-soluble trapped 14C from palmitate oxidation were measured following a 30-min incubation of viable mitochondria in a sealed system, as described previously (4, 10, 19). Briefly, viable mitochondria (100 μl) were added to a system containing a pregassed modified Krebs-Ringer buffer supplemented with 5 mM ATP, 1 mM NAD+, 0.5 mM d,l-carnitine, 0.1 mM coenzyme A, 25 μM cytochrome c, and 0.5 mM malate. A microcentrifuge tube, containing 500 μl of 1 M benzethonium hydroxide inserted into a 1.5-ml centrifuge tube, was placed in the system to capture 14CO2 produced during the oxidation reaction. The system was then sealed with a rubber cap and further sealed with parafilm. The reaction was initiated by the addition of a 6:1 palmitate-BSA complex (containing 10 μCi of [1-14C]palmitate, for a final palmitate concentration of 77 μM) administered by syringe through the rubber cap. The reaction continued for 30 min at 37°C and was terminated with the addition of ice-cold 12 M perchloric acid (PCA) by syringe through the rubber cap.
A fraction of the reaction medium was removed through the cap and analyzed for isotopic fixation. Gaseous CO2 produced from oxidation of [1-14C]palmitate was measured by acidifying the remaining reaction mixture. Liberated 14CO2 was trapped by the benzethonium hydroxide over a 90-min incubation period at room temperature. The tube containing the 14CO2 was put in a scintillation vial, and radioactivity was counted.
Whole muscle palmitate oxidation.
Whole muscle palmitate oxidation rates, expressed per gram wet weight, were calculated from mitochondrial palmitate oxidation rates, expressed per milligram of mitochondrial protein. To accomplish this, the mitochondrial recovery was first applied to the mitochondrial oxidation value, and subsequently this value was divided by the starting wet weight value.
Determination of blood metabolites.
Venous blood was sampled directly into vacutainers containing heparin and partitioned into two fractions. An aliquot of 200 μl of whole blood was added to 1 ml of 0.6 M PCA and centrifuged. The deproteinized supernatant was stored at −80°C and later analyzed for glucose (2). A second aliquot of whole blood was immediately centrifuged, and the plasma was removed and stored at −80°C. The plasma was later analyzed for free fatty acids (Wako NEFA C test kit; Wako Chemicals, Richmond, VA) and insulin (RIA insulin kit; Cedarlane Laboratories, Hornby, ON, Canada).
Western blot analysis.
Whole muscle crude membranes were generated as previously described (5, 6, 27) and analyzed for total protein (BCA protein assay), and 35 μg of denatured protein were loaded for Western blotting. Purified isolated mitochondrial fractions were analyzed for total protein (BCA protein assay), and 25 μg of denatured protein from each sample were loaded for Western blotting. All samples were separated by electrophoresis on 8% SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride membrane. The FABPpm polyclonal antibody used was produced in the laboratory of Dr. J. Calles-Escandon (11, 26). The MO-25 antibody used to detect FAT/CD36 was produced in the laboratory of Dr. N. N. Tandon (28). This antibody has also been used previously in our work (5, 10, 19). Commercially available antibodies were used to detect cytochrome c oxidase IV (Cox-IV; Invitrogen, Burlington, ON, Canada), GLUT4 (Chemicon International, Temecula, CA), caveolin-3 (Cav-3; BD Biosciences, Mississauga, ON, Canada), sarcoplasmic reticulum calcium-ATPase (SERCA; Affinity Bioreagents, Golden, CO), fatty acid transport protein 4 (FATP-4; Santa Cruz Biotechnology, Santa Cruz, CA), and hormone-sensitive lipase (HSL; ProSci, Poway, CA). An internal control of previously extracted human muscle crude membrane was used in each gel. Blots were quantified using chemiluminescence and the ChemiGenius 2 Bioimaging system (SynGene, Cambridge, UK).
All data are presented as means ± SE. Differences between lean and obese participants were analyzed with an unpaired, two-tailed t-test. Associations between variables were investigated using Pearson correlation analyses, as appropriate. Correlations were done by including both lean and obese individuals. Statistical significance was accepted at P < 0.05.
Subject characteristics.
There were no differences between lean and obese participants in mean age, fasting blood glucose, or fasting free fatty acid concentrations (Table 1). In contrast, there was a significant (P < 0.05) increase in body mass, BMI, and fasting insulin concentrations in the obese participants (Table 1).
Effect of obesity on whole muscle fatty acid transport protein contents and fatty acid oxidation enzymes.
Obesity did not alter the whole muscle protein expression of FAT/CD36, FABPpm, FATP-4 (Fig. 1, A–C, respectively), or HSL (Fig. 2A). In contrast, the whole muscle activity of β-HAD and CS, as well as Cox-IV protein content, were reduced (P < 0.05) by 17, 34, and 32% (Fig. 2, B–D, respectively). CS activity negatively correlated with BMI (r = −0.53; P < 0.05); however, β-HAD (r = −0.31), HSL (r = 0.38), and Cox-IV (r = −0.26) did not.
Effect of obesity on skeletal muscle mitochondrial proteins.
The mitochondrial recovery was not different between lean and obese participants (21 ± 1 vs. 23 ± 1%), nor was the viability of the mitochondrial preparation (92 ± 1 vs. 94 ± 1%). The absence of SERCA (110 kDa), GLUT4 (45 kDa), and Cav-3 proteins (23 kDa) indicated that the isolation procedures successfully yielded highly purified mitochondria without contamination from other membrane or intracellular sources. In addition, the isolated mitochondria exhibited significant expression of FAT/CD36, FABPpm, and Cox-IV contents (Fig. 3, A–C, respectively) that did not differ with obesity.
Effect of obesity on whole muscle and mitochondrial fatty acid oxidation.
Obesity did not alter the rate of palmitate oxidation in isolated mitochondria (Fig. 4A). In contrast, palmitate oxidation calculated in the whole muscle was reduced in obese individuals (Fig. 4B). The rate of palmitate oxidation in isolated mitochondria did not correlate with BMI (r = 0.02; Fig. 5A). However, independently of BMI, palmitate oxidation in isolated mitochondria significantly (P < 0.05) correlated with mitochondrial FAT/CD36 content (r = 0.67; Fig. 5B) but not with mitochondrial FABPpm content (r = 0.42). In addition, calculated whole muscle palmitate oxidation was significantly correlated (P < 0.05) with whole muscle CS activity (r = 0.53) and β-HAD activities (r = 0.50).
Mitochondrial dysfunction has been proposed to explain reductions in fatty acid oxidation in skeletal muscle of obese individuals, leading to elevations in fatty acid storage and ultimately insulin resistance (14–16, 23–25, 34). Although there is evidence in support of this hypothesis (22, 24, 25, 31, 35), these findings are not uniformly supported (7, 8, 30, 36, 40). The main novel findings of the present study were as follows: 1) mitochondrial fatty acid oxidation rates were not different in obese vs. lean individuals; 2) the mitochondrial contents of FAT/CD36 and FABPpm were similar in the two groups; 3) whole muscle fatty acid oxidation rates and mitochondrial content (whole muscle CS, β-HAD, and Cox-IV) were reduced in obese individuals; 4) total muscle expression of FAT/CD36, FABPpm, and FATP-4 were not altered with obesity; and 5) mitochondrial fatty acid oxidation rates were highly associated with the content of FAT/CD36 on mitochondrial membranes. Therefore, we suggest that obesity-related impairments in fatty acid oxidation are a result of reductions in mitochondrial content and not an inherent dysfunction of the mitochondria to oxidize fatty acids. In addition, at the level of the mitochondria, the capacity to oxidize fatty acid is related to the availability of FAT/CD36.
Whole muscle protein content.
In the present study we found that whole muscle FAT/CD36 and FABPpm are unaltered with obesity. This corroborates our earlier finding that whole muscle FAT/CD36 is not decreased with obesity (7). However, our group previously reported that obesity resulted in a small (∼25%) reduction in whole muscle FABPpm and increased with type 2 diabetes (7). This discrepancy might have arisen because of sample population variation as a result of small sample sizes, and the current data suggesting that obesity does not alter homogenate FABPpm fits with the literature pertaining to other fatty acid transporter proteins (1, 7). In addition, for the first time we report that whole muscle FATP-4 in lean and obese individuals does not differ, and this is in agreement with Bandyopadhyay et al. (1). Previously, we reported that obese individuals repartition FAT/CD36 to the plasma membrane, thereby increasing fatty acid transport into the muscle (7). Although Bandyopadhyay et al. (1) also reported an increased amount of FAT/CD36 on plasma membranes in obese type 2 diabetics, their results are more difficult to interpret, because they also showed a proportional increase in FAT/CD36 in the intracellular compartment. This inconsistency might result from the different sexes studied or the skeletal muscle sampled. In the present study females were used exclusively and the rectus abdominus was sampled, whereas Bandyopadhyay et al. (1) used primarily male participants (20 male and 1 female) and sampled from an unspecified muscle biopsy (probably the vastus lateralis) (1). Regardless of these discrepancies, it is becomingly increasingly clear that the transport of fatty acids into skeletal muscle is increased with obesity and type 2 diabetes. Interestingly, FATP-4 appears to follow a similar trend, since type 2 diabetics also have increased levels of FATP-4 on the plasma membrane fraction (1).
Whole muscle fatty acid oxidation, enzymes, and mitochondrial volume.
The current data suggest that obese individuals have an impairment at the whole muscle level to oxidize fatty acids, corroborating the findings of others (20, 25, 38). In addition, our whole muscle palmitate oxidation values correlated with whole muscle CS and β-HAD activities, further suggesting that mitochondrial content determines the rate of whole muscle fatty acid oxidation. For fatty acid oxidation to be impaired in obese individuals, either mitochondrial content must be substantially reduced and/or the ability of the mitochondria to oxidize fatty acids must be impaired. We corroborate the findings of others that obesity is related to reductions in CS and β-HAD activities, as well as a decrease in Cox-IV content (24, 25, 31, 35). Although various cofactors and covalent and allosteric regulators can influence the kinetics of enzymes, the maximal activity of an enzyme is indicative of the total amount of the protein. Therefore, reductions in CS and β-HAD maximal activities indicate a loss of the mitochondrial enzymes in skeletal muscle of obese individuals. Collectively, these data indicate that the mitochondrial content in skeletal muscle of obese individuals is reduced (21). Although a decrease in mitochondrial content can explain the observed reduction in whole muscle fatty acid oxidation, further measurements were required to determine whether this mechanism was solely responsible or whether mitochondrial dysfunction further exacerbated the impairment in skeletal muscle lipid oxidation in obese individuals.
Mitochondrial fatty acid oxidation and transport proteins.
Contrary to our hypothesis, there were no differences in the ability of mitochondria isolated from obese individuals to oxidize fatty acids. This suggests that mitochondrial dysfunction is not a mechanism for the observed reduction in whole muscle fatty acid oxidation and that a decrease in mitochondrial content is primarily responsible. We originally hypothesized that mitochondria isolated from obese participants would have a diminished ability to oxidize fatty acids, possibly as a result of a decrease in the content of FAT/CD36 (4, 10, 19) and perhaps FABPpm. Since mitochondria isolated from the obese did not differ in their ability to oxidize fatty acids, one would not expect the content of both transporters to be different. In agreement, the content of both mitochondrial FAT/CD36 and FABPpm did not differ with obesity. In addition, although mitochondrial palmitate oxidation rates in lean and obese individuals did not correlate with BMI, the rates did correlate highly with FAT/CD36 mitochondrial content. Interestingly, FABPpm did not correlate with mitochondrial oxidation, suggesting that it is not rate limiting for the transport of long-chain fatty acid (LCFA) into mitochondria. Although this needs further investigation, the current belief is that the main function of FABPpm is to transport reducing equivalents into the mitochondria, since it is identical to mitochondrial aspartate amino transferase (3, 37).
Previous work from our group using a specific inhibitor of FAT/CD36 suggested that FAT/CD36 is necessary for fatty acid oxidation at the level of the mitochondria (4, 10, 19). In addition, we have previously shown that the content of FAT/CD36 on mitochondria can predict the ability of mitochondria to oxidize fatty acids during exercise (19). Others have also shown that mitochondrial FAT/CD36 can be altered with training, and the training-induced increases in mitochondrial FAT/CD36 correlated significantly with changes in resting fatty acid oxidation (32). The current work further strengthens the evidence that FAT/CD36 has a vital role in the transport of fatty acids into mitochondria. We recognize that CPT I also has an important role in this level of regulation (4, 19), and further research is required to determine exactly how CPT I and FAT/CD36 interact to facilitate LCFA transport.
Previous data suggest that there may be a threshold required for impairments in whole muscle fatty acid oxidation to be manifested, i.e., BMI between 40 and 50 (20, 38). In the current study, our BMI value in the obese group was relatively high (mean: 37.6 kg/m2), but since they did not display mitochondrial dysfunction, it is possible that they were below this threshold. However, it was not directly determined in these previous studies whether mitochondrial function was impaired. In our study, the obese group displayed reductions in whole muscle CS and β-HAD activities, reduced Cox-IV content, and reduced whole muscle fatty acid oxidation rates, all of which indicate lipid oxidation was compromised. Thus the current data suggest that the reductions in fatty acid oxidation that have been associated with obesity result from reductions in mitochondrial content, and not from intrinsic mitochondrial alterations. This is an important distinction, because it suggests that interventions are required to increase mitochondrial biogenesis, but these do not have to "remodel" existing mitochondria. Indeed, previous work has shown that regular exercise can increase mitochondria volume in the obese population, as shown by increases in CS, β-HAD, and CPT I activity (9). In addition, associated with these changes in obese skeletal muscle are an increase in fatty acid oxidation and, subsequently, reductions in total DAG and total ceramides contents, as well as improved insulin sensitivity (9).
In the current study, obese participants displayed a trend (P = 0.12) toward an increase in the capacity of mitochondria to oxidize fatty acids. Previously, Bandyopadhyay et al. (1) showed that the capacity of mitochondria to oxidize fatty acids in obese type 2 diabetics (BMI = 36.93 kg/m2) was actually increased. Together, these studies suggest that a "reverse continuum" may exist in mitochondrial oxidation with obesity; that is, although whole muscle fatty acid oxidation may decrease with increasing BMI as a result of reductions in mitochondrial content, the capacity of the remaining mitochondria to oxidize fatty acids might actually compensate by increasing oxidation in an undetermined way to counteract this effect. Interestingly, the BMI of our obese participants was almost identical to that of Bandyopadhyay et al. (1), suggesting that the continuum might be based on insulin sensitivity, and not BMI. Importantly, it remains to be determined whether the diabetic increase in mitochondrial capacity to oxidize fatty acids is a beneficial response or, in combination with a decrease in electron transport chain capacity, will lead to increases in lipid peroxide species and, subsequently, oxidative damage that could exacerbate the diabetic phenotype (13, 17, 33).
We originally hypothesized that mitochondria from obese individuals would display an impaired ability to oxidize fatty acids. It has been suggested that the SS mitochondria from obese individuals may display a disproportionate impairment in fatty acid oxidation, since a greater reduction in the ratio of electron transport chain activity to mitochondrial DNA was observed in this mitochondrial fraction (31). The SS fraction represents only ∼25% of the total mitochondria in skeletal muscle (12). In the current study we pooled the SS mitochondria with the IMF mitochondria to ensure that adequate protein was recovered for our functional assays. Although this approach limited our ability to detect impairments in the SS mitochondria, in the previously mentioned study a significant reduction in the ratio of electron transport chain activity to mitochondrial DNA was also observed in IMF mitochondria and at the whole muscle level (31). Although this suggested dysfunction in all mitochondria, the present data suggest that mitochondrial dysfunction in fatty acid oxidation is not required for reductions in whole muscle fatty acid oxidation. It should be noted that only a small fraction of mitochondria were recovered during the isolation procedures (∼20%), and therefore it is possible that a dysfunction is present in the mitochondria not recovered. Since the various subcellular mitochondria were pooled in the current study, future research should focus on examining the potential differences in the SS and IMF mitochondria of obese individuals.
In conclusion, we have shown that obesity does not alter the ability of skeletal muscle mitochondria to oxidize fatty acids. We observed that obesity-related reductions in skeletal muscle fatty acid oxidation are attributable to reductions in mitochondrial content and not to intrinsic alterations or dysfunction within the mitochondria. We further showed that the content of FAT/CD36 on mitochondria is not different in obese muscle. However, FAT/CD36 significantly predicts the ability of mitochondria to oxidize LCFA independently of BMI status.
This research was funded by the National Sciences and Engineering Research Council (NSERC) of Canada (to A. Bonen, D. J. Dyck, and L. L. Spriet) and by the Canadian Institutes of Health Research (to A. Bonen, D. J. Dyck, G. J. F. Heigenhauser, and L. L. Spriet). A. Bonen is also a recipient of a Canada Research Chair in Metabolism and Health, and G. Holloway is a recipient of an NSERC Canadian Graduate Scholarship.
We acknowledge the contributions of the surgeons, Drs. Lightheart, Loopstra, and Sibley.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2007 by American Physiological Society


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548 W 29th Street, Holland 49423
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This property has been Sold!
07/28/2016 at $150,000
This one owner home is located at the end of a dead end quiet street and sits on a beautiful partially fenced in large lot. The home has been lovingly cared for and has a new roof, siding, gutters, AC , furnace & kitchen appliances! There is a 3 season porch that leads off the 4th bedroom (or office/den area), a 2 stall garage, new carpet in the upper level, fresh paint a clean & dry full basement. Other features include original hardwood floors on the main floor, replacement windows, a newer hot water heater and underground sprinkling.
Property Information
- Status
- Sold
- Address
- 548 W 29th Street
- City
- Holland
- Zip
- 49423
- County
- Ottawa
- Township
- Holland City
- Total Finished SqFt
- 987
- Above Grade SqFt
- 987
- Garage Desc.
- 2, Detached
- Water
- Public
- Sewer
- Public
- Year Built
- 1953
- Taxes
- $1,663
Rooms and Land
- MasterBedroom
- 11X12 1st Floor
- Bedroom2
- 10X11 1st Floor
- Bedroom3
- 10X13 2nd Floor
- Bedroom4
- 10X12 2nd Floor
- Dining
- 1st Floor
- Laundry
- Lower Floor
- Living
- 12X18 1st Floor
- Other
- 12X12 1st Floor
- Basement
- Full Basement
- Cooling
- Central
- Heating
- Forced Air, Natural Gas
- Acreage
- 0.36
- Lot Dimensions
- 90x132x90x132
- Appliances
- Oven, Refrigerator
- Features
- Wood Floor
- Exterior Materials
- Vinyl
- Exterior Features
- 3 Season Room
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Listing Courtesy of @homerealty Holland
Office Address 11539 Lakewood Blvd Ste 70
Listing Agent Taressa L Sprick
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Our Michigan real estate agents can answer all of your questions about 548 W 29th Street, Holland MI 49423. Real Estate One, Max Broock Realtors, and J&J Realtors are part of the Real Estate One Family of Companies and dominate the Holland, Michigan real estate market. To sell or buy a home in Holland, Michigan, contact our real estate agents as we know the Holland, Michigan real estate market better than anyone with over 100 years of experience in Holland, Michigan real estate for sale.
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Clue: Tinker's target?
We have 1 answer for the clue Tinker's target?. See the results below.
Possible Answers:
Related Clues:
- Diamond middleman?
- To whom Tinker threw
- Noted name in civil rights
- Civil rights leader Medgar
- Tinker to ___ to Chance
- "Tinker to ___ to Chance"
- Tinker's target
- Middle of a noted Cubs trio
- Tinker-Chance connection
- Second baseman called "The Crab"
Last Seen In:
- New York Times - April 30, 2000
Found an answer for the clue Tinker's target? that we don't have? Then please submit it to us so we can make the clue database even better!


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Ultra Fire All-in-One Batteries Charger with Protected 2 x 18650 Rechargeable "3600mAh" Batteries
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To enable volume discounts on this site, use coupon code: BULKRATE during checkout. You will see a discount applied at the bottom of the shopping cart. Competitive pricing is available. Contact us for details.
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Share your reviews, photos and videos here!Review Program
Pros: excelente durabilidad de las baterías. Con: cargador viene con un solo tipo de cable de patas planas el 2nchufe.
pode comprar que vale a pena
Correct time of load
Pros: they charge very quick and they work for a long period of thime on my father's ultrfire flashlight. Charger works ok and the price and quality are very good, specially since they are not so easy to come by at every... Read more
Tiene buen precio, el cargador tiene posibilidad de cargar diferentes formatos de baterias. Las pilas creo que son algo flojas.
it is what it is ;) charger and 2 batterys. well made and it got led indicators (red/green) its not a con but it comes with us standard... Read more
-Good battery capacity for this price. -dual charger input: 100-240 or 12v. -It handles 18650 batteries with protection board. -Led (red... Read more
I got this as a charger and as replacement batteries for the flashlight 125390. The flashlight is fantastic, but the batteries that came... Read more
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Legend: Academic word (?) New word
You should spend about 20 minutes on this task.
The chart below gives information on the global sale of hybrid vehicles (vehicles that use both fuel and electricity) between 2006 and 2009.
Summarise the information by selecting and reporting the main features, and make comparisons where relevant.
Write at least 150 words.
You should spend about 40 minutes on this task.
Write about the following topic.
Inventors are not as important to society as doctors.
To what extent do you agree with this statement?
Give reasons for your answer and include any relevant examples from your own knowledge or experience.
Write at least 250 words.
The chart shows how many hybrid vehicles were sold in Japan, the US and the rest of the world from 2006 to 2009. During this time, total sales more than doubled from 360,000 to 740,000. However, the trends in the US and Japan were very different.
According to the data, sales were high in the US, but the pattern fluctuated. While they rose by 100,000 from 2006 to 2007, they peaked in 2007 at 350,000. After that, there was a steady fall to 280,000 in 2009. On the other hand, sales in Japan were much lower at the start. They rose gradually to 90,000 in 2008, but then they jumped to 330,000 in 2009. Sales in the rest of the world increased steadily from 50,000 to 120,000 during the four-year period.
Overall, the data show that more hybrid vehicles were bought in the US than in any other country. However, in 2009, Japan overtook the US and became the country where most vehicles were sold.
(166 words)
Inventors are creative people who develop new ideas and products, whereas doctors are specialists who make us feel better when we are ill. I believe that both types of people are important to society, but in different ways.
Inventors have had an enormous impact on our lives. In the past, they developed things like electricity, television and aeroplanes. These transformed people's home lives and working conditions and enabled them to travel more quickly to other countries. More recently, inventors have created computers, the World Wide Web and many other technological products that we now rely on completely. For these reasons, most people would agree that inventors are important people.
Doctors, on the other hand, save lives. They train for many years and they develop knowledge and skills that ordinary people do not have. If we get ill, we need someone who knows what is wrong and can tell us what medicine to take. If we go to hospital, we expect doctors to look after us. Everyone depends on doctors in this way all through their life.
Although we cannot live without doctors, it is also true that we cannot live without inventors. In fact, they also depend on each other. Doctors use inventions to help them in their work, and inventors get ill and need doctors to cure them.
To conclude, I think doctors and inventors are equally important. Like many people in society, they play an important part in human progress. So it is impossible to say who is more important.
(252 words)


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ALL good things take time, according to the old saying. If this is true, then the much-heralded Gonski review into Australian education system must be one of the greatest things in our nation's history.
According to a state government study, the Gonski review's recommended single funding system won't be delivered across all Australian schools until 2094.
In other words, the system won't apply universally until today's schoolchildren have children of their own, and those children also have children, then they have children.
It may come as a shock to these future Australians when, 81 years from now, they will suddenly be introduced to a decades-old educational reform composed by people who are no longer living.
For his part, federal Education Minister Peter Garrett disputes the state government's claim, but still anticipates a significant delay in Gonski implementation.
"We want to see schools funded under the new Schooling Resource Standard as soon as possible," he said yesterday, "which is why there will be a six-year transition period."
That is still a significant amount of time; enough time, obviously, for students to progress from primary to secondary school, or secondary school students to leave and either join the workforce or go to university.
Time is already rushing by. As state Education Minister Adrian Piccoli points out, it is now one year since the report's release, yet the federal government has not provided costing details about funding for the Gonski reforms.
There is a lesson in this for school students, and for all who study Australian politics.
It could provide insights even for those far-distant students in the class of 2094.
Originally published as Plans for schools borders on farce


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Niebel was born in Hamburg on 29 March 1963. After his Fachhochschulreife (college entrance qualification) in 1983, Niebel lived for one year in a Kibbutz in Israel. Later he served for eight years as an airborne infantry officer in the Bundeswehr in Calw. He then studied at the German College of Public Administration in Mannheim and finished his studies in 1993 as Diplom-Verwaltungswirt (similar to a Master of Public Administration degree). From 1993 to 1998, he worked at an employment bureau in Sinsheim, a part of the Federal Employment Office of Heidelberg.
Niebel is married and has three sons.
In 1990, he joined the FDP and was co-founder of the Heidelberg division of the Junge Liberale. Since 2003 Niebel has been a member of the Federal Board of the FDP and of the curatorship of the Friedrich Naumann Foundation. On 5 May 2005, the Federal Board elected him secretary general of the FDP.
Niebel has been a member of the Bundestag since 1998. From 2002 to 2005, he was chairman of the State group of Baden-Württemberg in the parliamentary group of the FDP. Since 1998, he has been the speaker of the parliamentary group on Labor Policy. In addition to this, Niebel has been a member of the group of German-Israeli parliament members since 1998. At the 2009 election, he stood unsuccessfully in the single member constituency of Heidelberg, but was elected from the land list.
From 2000 to 2010, Niebel served as the vice president of the Deutsch-Israelische Gesellschaft (German Israeli Society).
In January 2011, Niebel met with Israeli Deputy Foreign Minister Danny Ayalon and signed a joint Declaration of Intent aimed to increase bilateral cooperation in effort to assist developing nations, agreeing to work towards the rehabilitation of the contaminated Lake Victoria in Kenya, the main source of water for several states and one of the sources of the Nile River. In February of the same year Ayalon and Niebel met again to examine aid to the new Republic of South Sudan.


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"Disappointing service offered to patients"
About: NHS Lothian NHS Lothian Edinburgh EH16 4SA
Posted by Blues (as ),
As a hospital doctor, I am shocked at the service offered to patients by Blue practice, Stockbridge Health Centre.
in my opinion, they are at least a decade out of touch with modern patient care and expectations.
Please seek feedback from your patients about what they really want and need. Fast.
Do you have a similar story to tell? Tell your story & make a difference ››
Response from Jeannette Morrison, Head of Patient Experience, NHS Lothian 15 months ago
Dear Blues,
I was sorry to read your feedback about your recent experience with your GP practice. As you may know GP practices are independent and if you would like to make a complaint and or provide feedback you are encouraged to contact the Practice Manager at the practice directly who would be able to help you.
Alternatively, if there is anything more that l can do to help please get in touch with me at the Patient Experience Team:
Email: email@example.com
Telephone: 0131 536 3370
Post: 2 - 4 Waterloo Place, Edinburgh.
Kind regards
- {{helpful}} of {{total()}} people think this response is helpful
- Was this response helpful? Yes | No
Thank you for your feedback.
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The Madurai Bench of the Madras High Court has directed the Tamil Nadu Teachers Recruitment Board to revalue the answer-sheets of all candidates who appeared for PG Assistant Examination-2013.
The court also asked the Board to delete some questions and award marks where it (court) disagreed, while rejecting the objections pertaining to many other questions.
Justice S Nagamuthu, disposing of a batch of petitions, directed the Board to revalue the answer-sheets as per the expert committee's findings and also consequential orders passed in various petitions pertaining to it and then publish the names of candidates for certificate verification.
The judge said the interim order earlier granted directing the Board not to publish the results, after certificate verification, shall continue till fresh results were published after revaluation of answer papers.
The judge also dismissed eleven petitions challenging four questions, filed later, and seeking to direct the Board to revise the key answers and award marks for the Tamil Nadu Teachers Eligibility Test Exam - 2013 held on August 18.
The judge noted that the Board issued the tentative key answers, calling for objections. Thereafter experts considered the objections.
Based on their opinion, the final key answers were published on the official website. The results were published on November 5.
After publication of the results, a number of petitions were filed before this court which found prima-facie case in favour of some of the petitioners and decided the challenges in respect of at least around 65 questions.
Observing that the court was of the view that it cannot go on entertaining the petitions challenging the key answers, he said, "I make it very clear that in respect of this matter, we cannot entertain endless litigations", and dismissed them.


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my hair is naturally blond and I get light highlights, and I'm thinking about stripping the color out, but I've heard its really damaging..so have you ever done it? what was your hair like afterwards?
Most Helpful Girl
you mean bleach it? I've streaked my hair...it's naturally dark brown---kinda chocolatey brown and I've gone with platinumblond streaks, with graded colours the make it all blend it---so multi-toned---yeah, it's really harsh on your hair,what I did was put lots of conditioner on it every day & it worked out. just make sure you super-condition it before you get it done.^_^11|00|0


text: Brent Popolizio's #1 Fan's Live Journal (upickaddict) wrote in upickfanforum, 2003-03-02 17:24:00 Brent Popolizio's #1 Fan's Live Journal upickaddict upickfanforum 2003-03-02 17:24:00 Previous Share Next Mood: cheerful Information on Moto Star! Alright, Moto Star is Brent's band that he drums for. If you go to http://www.motostarnyc.com and e-mail Victoria Gross, the singer for the band, you can buy their old EP, Looking For Someone as well as the new EP. They have a new website coming soon and this one is there until they get it up and running. CD'S are ONLY 12 dollars a piece and well worth it because its interesting music! Please check them out if you can. You can e-mail Victoria at email@example.com and tell her that Stella from Brentaholics sent ya :) Be sure to check it out everyone! Subscribe Post a new comment Error We will log you in after post We will log you in after post We will log you in after post We will log you in after post We will log you in after post Anonymously switch LiveJournal Facebook Twitter OpenId Google MailRu VKontakte Anonymously default userpic Post a new comment Preview comment Help 0 comments Post a new comment 0 comments


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- Trending Now: More Trending:
- repugnant white nationalist, white supremacist bereft impanel cosmopolitan
Medical Dictionary
alternative splicing
alternative RNA splicing
Medical Definition of alternative splicing
: a mechanism in which different combinations of exons are joined together during the final stages of transcription so that more than one messenger RNA is produced from a single gene In complex genes, alternative splicing can generate dozens or even hundreds of different mRNA isoforms from a single transcript.—Vivien Marx, Genomics & Proteomics, May 2004—called also differential splicing ◆Alternative splicing produces different messenger RNAs especially through the removal of one or more exons. Since each messenger RNA produced forms a different protein product, this mechanism enables a gene to encode multiple proteins that are structurally and functionally distinct.
alternatively splicedadjective
Seen and Heard
What made you want to look up alternative splicing? Please tell us where you read or heard it (including the quote, if possible).


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Features include four rear-mounted XLR-F mic inputs plus an XLR-m output to feed the preamp. To eliminate bleed between microphones, each channel is equipped with a 'radio style' selector switch that ensures only one mic is on at a time. The external supply provides power for the LED indicators, charges the relays and enables the Gold Digger to generate 48 volt phantom which can be turned on or off for each mic as needed. To ensure an 'honest' comparison between microphones, individual 'set & forget' trim controls let you adjust each mic level so that they produce the same relative output. Once set up, the Gold Digger not only improves workflow, but also gives the artist more confidence with the sound of the vocal, resulting in a better overall performance.
The Gold Digger – A tool designed to get the very best out of each and every performance.


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I keep hearing blurbs about the mysterious Episode 310, and the supposed script that Episode 310 is supposed to have.
What people aren't realizing is that this "script" is one of SEVERAL sample scripts posted by Dan on his blog in September 2009 (yes, over a full year ago!) called "When Fans Assume". It's Freddie and Carly talking about who Freddie is in love with, and there are FOUR, yes count them--FOUR sample scripts. Two have him in love with Carly, another with Sam and then another with *drumroll*--Gibby.
So this isn't really a new script or a sign for any of the ships.


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- Description
DetailsCreate a casual fashion statement to your dining area with these rich brown side chairs. With the seats and back upholstered in a padded brown leather-look material, accent stitching, and square tapered legs, these chairs will provide extra comfort while bringing a contemporary appeal.
- Additional Information
Additional Information
SKU 26700166 MPN I 1936 GTIN UPC 021032232948 Brand Monarch Specialties Collection N/A Assemble Length 19 Assemble Width 23 Assemble Height 40 Assembly Required Yes Material Wood Finish Brown Styles Modern Shipping Time 1-2 Weeks Free Shipping Yes
- Reviews
Disclaimer: Pictures displayed may include optional products not included in the price. Computer monitors often display color variations. Color swatches are shown if alternative colors are available. Please call us or email us if you have any questions!


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Cow Mandi 2016 Lahore Live Streaming by Bakra Mandi PakistanCow Mandi 2016 Live Stream with Bakra Mandi Pakistan Admin's. One of the Best Feature Introduced by Facebook for Pages is Live Stream which Bakra Mandi Pakistan Team used for the First time and Response was extremely Fabulous.
We Showed our Members young and old Bulls for sale in Cow Mandi. We mostly Show Medium size Bulls in our Videos as we know most people can buy medium size bulls so we showed you people what rate is Mandi and Seller (Biyopari's) are demanding.
Bakra Mandi Pakistan Showed You in Live Streaming which NO Other Facebook or Website will Show You.
Difference Between Bakra Mandi Pakistan and CompetitorsBakra Mandi Pakistan team is working more and more and trying to give some extra information which till now no other facebook page or Website is updating about but soon they will Copy us.
We ask Buyers to tell us the price which Seller (Biyopari) was Demanding, then what was your Starting Price and then what was the Actual Price you Bought or you Ended Up as Seller is not reducing the price. Performing this whole activity gives us and our Members an idea that how much difference a Biyopari can make and give you the Bull. This thing is also helpful for people who are going to buy Cow or Bull from Cow Mandi for the First Time.
Your Suggestion's are Always Welcome
Bakra Mandi Pakistan's Admin's always strive to update our members with question's like what is the Rate Difference from previous Year, or most of our Facebook Page Members asks that could we buy in the same price as previous year or we have to arrange some extra money this Year.
So to answer your question's we Managed to give you Live Stream Transmission from Cow Mandi and this is the Video you Must Watch and Comment Below.
For More Information or Any Kind of Suggestion Kindly Like Bakra Mandi Pakistan Facebook Official Page.
Mandi live session
Posted by Bakra Mandi Pakistan on Saturday, August 20, 2016