text:
Helpful (3)
New America is a challenging and interesting place to work and prioritizes intellectual achievement and growth for its employees. Getting to work in a positive atmosphere with smart, thoughtful, ambitious colleagues is a rare and fortunate thing.
Like most non-profits, New America has some management challenges. In particular, the pace of overall organizational growth has not always been met by equivalent growth of support staff which means there can be a strain on the resources.
I worked at New America full-time (More than a year)
Flexible, not bureaucratic, smart people
Lack of cohesion between programs
Helpful (7)
Near the center of power; lots of glitz and talented people. Great networking environment combined with an opportunity to make a difference in the world. Excellent pay, especially if you're a good fundraiser for your program. Neither a liberal nor conservative think tank, nor one that is primarily corporate or labor dominated.
Not a good place to work if you're a serious researcher. If you're on the fellow/journalistic side of the foundation, you get a fair bit of independence, especially if you've got a high profile byline. If you're on the program side, expect to toe the line of your program, its fundraisers, and its political allies; in other words, work on the program side has a lot in common with work at an advocacy group in DC. At New America, it's usually not necessary to tow an ideological line such as at Heritage (conservative/Republican) or Center for American Progress (liberal/Democratic). But there are plenty of other lines to tow that an academic should feel uncomfortable with.


text:
zenkat Review
Didn't love it- returned this one
This comes in a hundred pieces and that I wasn't aware of that nor do I have the time or patience to spend hours putting it together. Te crystals and shade are really nice but the metal looked like very cheap fake chrome to me. And I suspect it won't hold up over time. I returned this one.
- How did the color on site compare with the actual product? Exact
- Please tell us about the quality of the product. The silver metal looked and felt cheap but the shade and crystals were nice.
- How accurate was the on site description of the product? Semi accurate
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The abbreviated name, 'mfold web server', describes a number of closely related software applications available on the World Wide Web (WWW) for the prediction of the secondary structure of single stranded nucleic acids. The objective of this web server is to provide easy access to RNA and DNA folding and hybridization software to the scientific community at large. By making use of universally available web GUIs (Graphical User Interfaces), the server circumvents the problem of portability of this software. Detailed output, in the form of structure plots with or without reliability information, single strand frequency plots and 'energy dot plots', are available for the folding of single sequences. A variety of 'bulk' servers give less information, but in a shorter time and for up to hundreds of sequences at once. The portal for the mfold web server is http://www.bioinfo.rpi.edu/applications/mfold. This URL will be referred to as 'MFOLDROOT'.
The concept of RNA secondary structure began with the work of Doty and Fresco (1,2). The prediction of RNA secondary structure (folding) by energy minimization using nearest neighbor energy parameters began with Tinoco and colleagues (3–6) and also with Delisi and Crothers (7). Efficient algorithms for RNA secondary structure prediction using dynamic programming methods borrowed from sequence alignment were developed independently by a number of people (8–13).
My own early RNA folding programs (12,14) computed a single minimum energy folding of an RNA sequence. They were popular in the 1980s and a modified version was incorporated into the UWGCG (University of Wisconsin Genetics Computer Group) suite of programs (15); the acronym was shortened to GCG when this group left the University of Wisconsin to form a private company. Initially, they used free energy parameters that had been summarized by Salser (16). After 1986, free energies from the Turner group (Doug H. Turner, Department of Chemistry, University of Rochester, Rochester, NY) were used (17).
The 'mfold' software for RNA folding was developed in the late 1980s (18). The 'm' simply refers to 'multiple'. The core algorithm predicts a minimum free energy, ΔG, as well as minimum free energies for foldings that must contain any particular base pair. Any base pair, ri−rj, between the ith nucleotide and the jth nucleotide that is contained in a folding no more than δδG from the minimum, is plotted in a triangular plot called the 'energy dot plot'. The base pair ri−rj is plotted in row i and column j of this matrix. The free energy increment, δδG, is chosen a priori by the user, who selects a 'percent suboptimality', P. From this, δδG is computed to be P/100 |ΔG|. Base pairs within this free energy increment are chosen either automatically, or else by the user, and foldings that contain the chosen base pair are computed. They have minimum free energy conditional on containing the chosen base pair. The description and use of the mfold package has appeared in a number of articles (19–22). The closely related 'RNAstructure' program has also been described (23,24).
The Turner group has published numerous articles over the years that detail the development of the RNA folding parameters. A subset of these articles are what I would call 'major works' that summarize the current state of the art. Version 1 of the mfold package used free energies that were described by Freier et al. (17). Versions 2.1 to 2.3 used the parameters from Walter et al. (25), although the incorporation of coaxial stacking parameters into the minimization algorithm has not been accomplished. The current version 3 software uses free energy data from Mathews et al. (26).
DNA folding prediction with the mfold software began in 1996, when DNA specific parameters were added to the mfold package through a collaboration with the SantaLucia group (John SantaLuica Jr., Department of Chemistry, Wayne State University, Detroit, MI). These data have been described by SantaLucia (27). The DNA stacking (27), single mismatch (28–32) and dangling end (33) parameters have been measured in the SantaLucia laboratories. The remaining terminal stacking and loop parameters were estimated by SantaLucia and have been incorporated into the mfold package by personal communication from John SantaLucia. They remain unpublished. In 1999, corrections for [Na+], [Mg++] were incorporated into the mfold package (34).
The mfold web server was first created at Washington University School of Medicine during the fall of 1995. DNA folding parameters were added in the spring of 1996. From 1995 until the fall of 2000, the server ran on SGI workstations or multiprocessor servers (Silicon Graphics, Inc. 1600 Amphitheatre Pkwy., Mountain View, CA 94043), as well as on a dual processor 'Intel/Solaris' platform (Pentium 2 processors by Intel Corp., Solaris operating system by Sun Microsystems). The server was moved to Rensselaer Polytechnic Institute (RPI) in October 2000. It ran on a dual processor 'Intel/Linux' platform (Linux operating system as developed and marketed by Red Hat, Inc., 1801 Varsity Drive, Raleigh, NC 27606). Since July 2002, the mfold web server has been running on a cluster of 36 dual processor 'Intel/Linux' workstations that were donated to the joint RPI-Wadsworth Bioinformatics Center by IBM (IBM Research, P.O. Box 218, Yorktown Heights, NY 10598). This equipment was awarded as an SUR (Shared University Research) grant to RPI and the Wadsworth Center (PI: M. Zuker).
The use of the mfold web server has grown steadily since its inception. The 'quikfold' server was added while I was still at Washington University. The remaining servers that will be described have all been added within that past 2 years. The servers have been used extensively by researchers in universities, medical schools, non-profit organizations, US government and military laboratories and by companies all over the world. In addition, the servers are being used in the teaching of computational biology methods.
The mfold web server comprises a number of separate applications that predict nucleic acid folding, hybridization and melting temperatures (Tms).
The original applications on the mfold web server deal with folding a single RNA or DNA sequence per submission (job). The submission forms for RNA and DNA are separate for historical reasons only. RNA folding came first. These applications may be reached by following the 'RNA Folding' or 'DNA Folding' hyperlinks from the main portal or entrance page. The URL for the portal will likely remain stable, while those for separate applications will change. The default RNA folding form currently uses the latest version 3.0 free energies (26). These are recommended for most RNA folding. However, there is a link from this page to what is called the 'RNA mfold version 2.3 server'. This server offers RNA folding using the older, version 2.3 energy parameters (25). Why use older and less accurate parameters? The reason is that we have enthalpies for these older parameters. As with the free energy parameters, the enthalpies were measured at 37°C. However, they are assumed to be constant within the range of temperatures that might occur in vivo or in the laboratory. This enables the server to extrapolate free energies to other temperatures and to fold at these temperatures.
A sequence name may be typed or pasted (entered) within the 'Enter a name for your sequence:' text field. Long names are truncated to 40 characters. Any ASCII characters may be used, including those with octal values greater than 200Octal (O). The 'dangerous' characters, ", <, > and ', are converted to ', (253 O), (273 O) and ′ (264 O), respectively. The character, \, is eliminated. If no name is entered, then the sequence name becomes the 'Job ID', which is of the form yyMmmdd-hh-mm-ss, where yy is year, Mmm is month, dd is day, hh is hour, mm is minute and ss is second. For example, a job that comes into the server at 8:23:06 pm local time on 9 February 2003, will be assigned a Job ID of 03Feb09-20-23-06. If two jobs come in during the same second, the second one has the letter 'a' appended to it, and so on for more than two jobs during the same second.
A sequence must be entered into the sequence text area box. All characters except for 'A–Z' and 'a–z' are removed. Lower case characters are converted to upper case. For RNA folding, 'T' or 't' are converted to 'U', while 'U' or 'u' are converted to 'T' in DNA folding. If, for example, you enter
then the resulting sequence will be
rather than
The letter 'N' should be used for an unspecified base. It is not allowed to pair. The letters 'B', 'D', 'H' and 'V' denote 'A', 'C', 'G' and 'U/T' respectively. These nucleotides may pair only if their 3′ neighbor is unpaired. The purpose of this convention is to denote nuclease cleavage of the phosphodiesther bond linking the indicated nucleotide and its 3′ neighbor. It is used to constrain folding when nuclease digestion data specify susceptible bonds. In addition, the letters 'W', 'X', 'Y' and 'Z' also refer to 'A', 'C', 'G' and 'U/T', respectively. These nucleotides, if they pair, should do so only at the end of a helix. Thus, the mfold web server does not support the IUPAC (International Union of Pure and Applied Chemistry) ambiguous DNA character convention (35) shown in Table Table11.
The text area in the constraints box allow for the optional incorporation of folding constraints. Each constraint consists of a single line in the box that must conform to a rigid format. When constraints are used, a hyperlink labeled 'Explanation of sequence annotation' appears on the primary results page.
The various types of constraints are itemized below. Multiple constraints of any form are allowed in any order.
Folding sequences containing up to 800 bases may be done while the user waits. This is the default. At this time, folding results for immediate jobs are erased 24–26h after they are submitted. For sequences from 801 to 6000 bases in length, the batch option must be selected from the appropriate option button. The user should enter a valid email address in this case, although email addresses are always welcome since they identify users. For batch jobs, pressing the 'Fold RNA' or 'Fold DNA' submission button takes the user to a notification page that indicates a URL for the results and the email address to which a notification will be sent when the folding is completed. If the link to the results pages is followed immediately, then the target page will be incomplete. It will contain only the sequence and some other input information. When the folding is complete, this page must be 'refreshed' or 'reloaded' for the results to be seen. The URL for the results remains valid for 48–51h, after which the results are erased. Folding sequences of up to 10400 bases is available at special request. Folding 6000 bases currently takes about 1.5h. The hyperlink labeled 'View Folding Results' will enable a user to view any results on the server, provided that they have not been erased and that the user views from the same computer that generated the foldings. That is, the user's IP address must be the same.
Folding results may be viewed by following links from an initial results page. Some input information is reproduced at the top of this page in a text area. An example of the top portion of an initial results page is shown in Figure Figure11.
The energy dot plot is available in PostScript, png and jpg form. The 'Text' hyperlink leads to a plain text file that gives the basic dot plot information. The 'istart', 'jstart' and 'length' options correspond to the (i, j, k) numbers that define a helix, as defined previously. The 'energy' in an integer in units of tenth of a kcal/mol. Level 1 corresponds to helices in optimal foldings. Levels 2–4 correspond to the helices in increasingly suboptimal foldings. The levels from 1 to 4 correspond to the default coloring of the base pairs in the dot plot. The PostScript hyperlink leads to a static PostScript image of the energy dot plot. Letting δG be the minimum free energy of a folding containing a base pair, the default color (rgb Hex) of a base pair in the energy dot plot is given in Table Table44.
The png and jpg hyperlinks each lead to a new page containing a png or jpg image of the energy dot plot and buttons that allow the user to interact with or redraw the dot plot. It is called the primary dot plot page. A small 'Details' window, 550×40 (pixels) opens in the top left corner of the screen. With the 'Click on a base pair to identify' radio button selected (the default), clicking (left click) on the image will cause the (i, j) value of the selected base pair as well as its δG value to appear in the Details window. If a blank area of the plot is chosen, the value of δG will be declared 'Undefined'.
Selecting the 'Click on the image and redraw the dot plot with options below' button activates the text area and five option buttons below. Clicking on a point in the dot plot will cause a new image to be created. If the magnification factor is chosen to be >1, then the new image will be magnified about the selected point; otherwise the entire dot plot will be redrawn. The value of the 'Energy increment in kcal/mole' option will be an upper bound on δG. For example, setting it to zero will cause only optimal base pairs to be plotted. The number of different colors used in the dot plot may be changed. If k is the value of the 'Filter' option, then only helices of size at least k will be plotted when the dot plot is redrawn. The 'Image Width' controls the resolution (size) of the redrawn image as explained above. If the PostScript 'Output' option is chosen, then a static PostScript image is created. If the png or jpg 'Output' option is chosen, then clicking on the plot will cause a new window to open containing the redrawn image and the same set of control buttons as before. This is called the secondary dot plot window. New png or jpg images created from this secondary window will be drawn within the secondary window. Similarly, new png or jpg images generated from the primary dot plot window will refresh the secondary window. A typical use would be to magnify about a region in the initial dot plot. Base pairs in the magnified dot plot can then be selected with precision, allowing the user to identify specific helices that may occur in optimal or suboptimal foldings. Closing the secondary window will not affect other windows. Closing the primary window will also close the Details window.
Computed secondary structures are also available in the RNAML (37) format. This format has been proposed as a syntax for exchanging RNA structure information. We are currently offering output that is compatible with the DTD (Document Type Definition) file maintained by the Major group at the Université de Montréal. This file may be found at: http://www-lbit.iro.umontreal.ca/rnaml/current/rnaml.dtd.
These are raw text files that contain information that might be useful to some users. Annotation by p-num value is available on the server, as described above. The raw values are also made available. The h-num link contains h-num values of each helix in the energy dot plot, sorted from lowest to highest value of h-num. The h-num value of a helix is the 'well-definedness' of a helix (38). Helices with lower h-num values tend to be more reliably predicted. The log file for a run is sometimes useful in finding the cause of a failed job.
All computed foldings may be downloaded at once in a single zipped file (.zip) or a compressed tar file (.tar.Z). The structures may be taken as PostScript, png or jpg plot files, or as raw text files in different formats.
The text files will be useful to those who wish to create structure plots on their own. Some of the text formats have been described before (21,22). A full description may be found at MFOLDROOT/www-NAR03/doc/structure-format.html. Redrawn structures or structures created using the new 'sir_graph' program can only be downloaded individually.
The ss-count file contains explicit statistics on single strandedness of each base in all of the computed foldings. The first line contains the number of foldings that were computed. The ith subsequent line contains the number of the ith base, the base itself and the number of times it is single stranded in all of the computed foldings. The 'View plot' button creates a plot of base number versus ss-count. When the 'Averaging window' is m>1, then the value that is plotted for the ith base is the average over the m bases centered at i. Fewer bases may be used near the ends of the plot. The magnification factor enables the user to zoom in on a region of interest.
The hyperlink to 'Structure i' leads to a portion of a single html file that contains the ith structure in an easy to read text format. This format may be suitable for viewing small foldings. It may easily be pasted into text files. If the sequence is very large and the structure plot has low resolution, it might be more convenient to view a portion of the text file than to magnify a portion of the structure plot.
Selecting PostScript,.ct file, RnaViz ct, Mac ct, GCG connect or XRNA ss will lead to single structures in the designated format. Following the png or jpg link is similar to following the png or jpg links when viewing the energy dot plot. Both lead to a new page containing a png or jpg image of the structure plot and buttons that allow the user to interact with or redraw the folding. It is called the primary structure plot page. A small 'Loop Free-Energy Decomposition' window, 550×150 (pixels), opens in the top left corner of the screen. With the 'Click on a base pair to view related structural details in the Loop Free-Energy Decomposition window' radio button selected (the default), clicking (left click) on the image will cause the details of the selected base pair to appear in the small window. The window will show the type of loop, including a stack, that is closed by the selected base pair. The free energy of that loop is given, and the specific identity of the base pair is given. If the selected point does not unambiguously select a base pair, then the auxiliary window will print a message instructing the user to try again.
Selecting the 'Click on the image and redraw the structure with options below' button activates the text area and four option buttons below. Clicking on a point in the structure plot will cause a new image to be created. If the magnification factor is chosen to be >1, then the new image will be magnified about the selected point; otherwise the entire structure will be redrawn. The 'annotation' button allows the user to select p-num or ss-count annotation, even if these were not selected initially. The 'annotation type' button has no effect unless an annotation option is selected. In this case, an annotated structure will be drawn with colored base letters, with colored dots or with colored dots containing base letters, corresponding to the choices 'Character', 'Dot' or 'Both', respectively. The 'Image Width' controls the resolution (size) of the redrawn image, as explained above. If the PostScript 'Output' option is chosen, then a static PostScript image is created. If the png or jpg 'Output' option is chosen, then clicking on the plot will cause a new window to open containing the redrawn image. There are no buttons on this page. The 'Color Table' option leads to a table that explicitly shows the colors used in the structure plot versus the p-num or ss-count values. The p-num or ss-count file hyperlink leads to the corresponding raw text files. This is called the secondary structure window. New png or jpg images generated from the primary dot plot window will refresh the secondary window. A typical use might be to magnify about a region in the initial structure plot so that a portion of the structure might be printed at a suitable resolution. It could also help the user select base pairs with precision. Closing the secondary window will not affect other windows. Closing the primary window will also close the 'Loop Free-Energy Decomposition' window.
The structure plots that are drawn by default and that can be downloaded at once are generated for the most part by the 'naview' program by Bruccoleri and Heinrich (39). The 'naview' program creates a device independent ASCII plot file (with suffix plt2). Software developed for the mfold package at Washington University creates PostScript, jpg and png images from these files. A new drawing program named 'sir_graph' was created at Washington University for creating displays of nucleic acid secondary structure. It is an interactive program that runs on a variety of Unix platforms. A non-interactive version, 'sir_graph_ng', creates displays for the mfold web server. The 'Click Here for New Structure Viewing Options' hyperlink on the primary results page leads to a 'Structure Viewer' page that is equivalent to the primary window page described above. This page is identical to the ones that may be reached by following the 'new' hyperlinks in the 'View Individual Structures' section.
The user has a wider selection of choices with this new software. The external loop, or exterior base pairs, may be drawn in the default way, arranged around a circle, or may be drawn 'Flat' with all the helices that meet it being parallel to one another. The 'Flat_Alt' option draws successive stems at 180° angles from the previous. The background may be the usual white or black. The base pair symbol may be a 'dot' which is usual for the mfold web server, or a 'line', which is common in most secondary structure displays. The 'Algorithm' may be the 'Default' or 'Simple' type, both of which avoid overlaps of stems. The 'Default' and 'Simple' algorithms draw the multi-branch loops differently. The 'Natural' option draws every loop as a perfect circle, with the stems coming out at appropriate angles. This may produce pleasing results for small foldings, but will usually produce a hopelessly tangled mess for large foldings. As with the display of structures using the older naview program, the 'Loop Free-Energy Decomposition' window opens and allows the user to identify precise loops or stacks, together with their free energies and closing base pairs. Also, the secondary structure window page is available for magnifications about chosen points.
When temperature cannot be altered, the 'Thermodynamic Details' link leads to a portion of an html file that contains the entire decomposition of the particular folding into loops and stacks, together with their free energies and closing base pairs. Consecutive runs of base pairs are summarized as helices. Small portions of this file are displayed in the 'Loop Free-Energy Decomposition' window when the user is interacting with a png or jpg plot of a folding.
When temperature may be altered, then a more attractive page appears that gives the free energy, enthalpy, entropy and an estimated Tm. Tm is computed using a simple 2-state model. This assumes that the molecular is either folded as shown or else completely single stranded. Such an assumption is reasonable for short molecules. For larger molecules, it might be useful to refold near the predicted Tm. If the new Tm is 'significantly' larger, it indicates that the given folding can rearrange into another folding with a higher melting temperature. The server uses Tm=ΔH/ΔS, where ΔH and ΔS are the structure enthalpy and entropy, respectively. (Because of the units used for entropy, the actual formula is Tm=1000ΔH/ΔS−273.15 in °C.) The usual details table follows.
The 'Dot plot folding comparisons' option allows the user to view any subset of the computed foldings in a dot plot. This option is available when two or more foldings are computed. The user may select foldings to be displayed by selecting the corresponding radio buttons. The default is the display of the first two foldings. For convenience, an 'All' button has been supplied.
Base pairs that occur in all foldings are colored black. Those that occur in two or more, but not all foldings are colored grey. Otherwise, base pairs are assigned a unique color that depends on the structure. This system breaks down when more than 15 structures are computed.
Selecting the png or jpg option in the first button and pressing the 'Do the Comparison' option leads to a primary structure dot plot page that is very similar to the primary energy dot plot page. Clicking on a base pair with the default 'Click on a base pair to identify' radio button selected will show the identity of the base pair and list which of the computed structures contain that base pair. When the 'Click on the image and redraw the dot plot with options below' radio button is selected, a click on the dot plot will generate a new dot plot. The dot plot will be in a 'secondary' structure dot plot window if the png or jpg option is selected. This is very similar to the secondary dot plot window for the energy dot plot. The magnification, output format and image width parameters are the usual ones explained above for the energy dot plot. The 'Multicolor Overlap' option that may be selected will have the effect of drawing the otherwise grey dots in a multi-color mode that displays precisely what foldings contain that base pair. In the mfold web server, 'dots' are really plotted as (usually small) squares. A collection of trapezoids in different colors are drawn within the square region. These indicate which foldings contain that base pair. The dot plot must be drawn with a high enough magnification for this feature to be seen. It is not necessary when only two foldings have been computed. When three foldings are computed, the gray base pairs that are in structures 1&2, 1&3 and 2&3 are plotted in the lower left triangle in three distinct colors, so they may be identified without using the multicolor option.
Many users are not interested in the sophisticated viewing options that are available on the mfold web server. Some may be content with the easy to read text format or with the 'ct format' files that may be downloaded and used locally to create secondary structure plots. Many wish to fold many sequences at once under the same conditions. For example, a user may wish to predict foldings and melting temperatures for hundreds of short molecular beacons.
For this reason, the 'quikfold server' was created. It uses the same 'nafold2' program that folds sequences in the regular server, but in a different, multiple molecule mode. Only immediate jobs are allowed, with each sequence containing no more than 600 nucleotides. The theoretical upper limit for the number of sequences that may be submitted at once is 25000, but I would not advise submitting nearly that many! The upper limit depends on the Internet, browser settings (such as timeout) and other factors. I do know, for example, that 1000 sequences of length 100 each should pose no problem.
The input page contains buttons and text areas that have already been defined. The single new item is that one extra character is required for the sequence input box. Each of the input sequences must be separated by (at least) one semi-colon, ';'. All other characters, except for 'A–Z' and 'a–z', are removed in a preprocessing step.
The quikfold server gives text, ct, RNAML and thermodynamic details as output. In each case, the output is in a single, possibly large text file. The sequences are named by taking the given or default sequence name, and adding '_i' to each name, where i ranges from 1 to the number of sequences. The folding results are erased within 40min to several hours. An individual sequence that cannot fold will not abort the entire job. It will simply be skipped over in the output.
Some users want only the minimum folding energy of a sequence. For this reason, the zipfold server was created. The input page is similar to that of the quikfold server. Sequence lengths may be up to 800. In one instance, a user submitted many thousands of jobs; each containing 50 randomized sequences of length 500. The failure rate was less than 1%.
Because speed is important and so little information is being requested, the underlying code was simplified to run much faster. At the time of this writing, 378 tRNAs were processed in just over 11s. The actual performance depends on the server load and difficult to control Internet related factors.
The underlying code has been stripped of its ability to handle constraints. Moreover, the 'fill algorithm' has been truncated so that only an optimal energy or a single optimal structure may be computed. Newer, experimental versions, streamline the sequence input as well.
All the output appears on a single, primary results page. The ΔG values are arranged in a list. Those sequences that cannot fold are given a large positive folding energy. The current value is 10000.
Some users want a little more that just a minimum folding energy. They also desire an estimated Tm. In this case, the Tm server may be used. Only version 2.3 RNA folding parameters are available for this server, since enthalpies are required for estimating Tm. The input is the same as with the zipfold server.
The Tm server uses the same simplified folding program as the zipfold server, except that a minimum energy folding is computed in this case. The enthalpy, ΔH, of this folding is then computed using the appropriate nearest neighbor parameters. From this, it is easy to compute an entropy, ΔS and then Tm, using a 2-state model as described above.
In fact, this server was created to 'service' the 'OligoArray 1' program of Rouillard et al. (40). The OligoArray program 'hits' the server with single DNA sequences using a direct request created by a Java application. Subsequent versions of OligoArray will abandon this inefficient procedure.
The underlying mfold software has had numerous small changes added over the years to accommodate 'special requests' or applications. Many users have used the regular mfold software, either in 'stand alone' form or on the web, to simulate the hybridization of two strands of RNA or DNA. They have done so by taking two sequences, A=a1a2a3···am and B=b1b2b3···bm and creating a single sequence, S=a1a2a3···amn1n2···nkb1b2b3···bm, by concatenating A with B using some non-pairing characters, n1···nk as a linker.
There are two problems with this approach. The first, less serious problem, is that the k linker residues end up in a loop of some sort. If A and B hybridize perfectly, then this loop will almost certainly be a hairpin loop. An example of such a folding is given in Figure Figure2.2. The problem is that an incorrect hairpin free energy is applied to loop '2'. Instead, it should be treated as an exterior loop, like '1'. In addition, an initiation free energy, ΔI, needs to be added. This is 4.1kcal/mol for RNA at 37°C and 1.96kcal/mol for DNA at 37°C.
In order to accommodate such situations, the following feature was added to mfold. When three consecutive Ls occur in a sequence (L for Linker), they are recognized as a linker. If they occur in a hairpin loop, then this loop is treated instead as an exterior loop and ΔI is added as well. The output still contains the linker residues, but the value of ΔG is now correct.
The linker residues may be forced to be in a hairpin loop by forcing a simple hybridization of A with B. This may be accomplished by using the two constraints
These constraints forbid all intramolecular base pairs, so that the only allowed base pairs link A with B, forcing, among other things, the linker residues to be in a hairpin loop.
The more serious problem, however, is that any estimated Tm is nonsense, since total nucleic acid concentration, C, must be taken into account when two strands hybridize. The correct 2-state estimate for Tm is
where f=2 if A≠B and f=4 if A=B.
All of this is accomplished automatically by the hybridization server. The two sequences, A and B, are entered into the sequence box text area, separated by a semi-colon, ';'. The total nucleic acid concentration must be given. The result is a simple output page containing the job ID, ΔG, ΔH, ΔS and Tm in a single line. No units are given, although they are understood to be kcal/mol, kcal/mol, cal/(K·mol) and °C, respectively.
The mfold web server is open to all users. No restrictions are applied to commercial users. However, users should be aware that the server is not secure and that data flowing both in and out may be detected by others. Moreover, query information is stored indefinitely in log files on the server. These log files are treated as confidential information, although gross statistics on usage are collected and disseminated. Furthermore, some of the submissions are selected as examples for teaching, but only if database searches reveal that the sequence is already in a public database.
The regular mfold server may not be hit by 'GET' requests. Only the 'POST' operation is permitted. The other applications that have been described may also be hit with 'GET' requests. Hitting these other applications directly using, for example, Java applications is permitted and will continue to be permitted until such time as the server is overloaded. This has not happened yet. At Washington University, perhaps 100–200 folding jobs were submitted each work day, with far fewer on weekends. For the month of December, 2002 (for example), I counted 21688 submissions to the regular mfold web server, 3128 to quikfold, 35904 to zipfold, 193428 to Tm and 542 to the hybridization server.
The current web server is running on equipment donated to RPI by IBM Research in the fall of 2001. The detailed hardware specifications are given in Table Table5.5. The total value of the donated equipment comes to about $214000. We contributed another $5000–$10000 for a switch and a custom designed power source. All equipment was originally assembled and housed at the Academy of Electronic Media (http://www.academy.rpi.edu/). The server is expected to be moved to the Voorhees Computing Center during the spring or summer of 2003.
It is our intention to keep the RNAML output up-to-date and in accord with the DTD file: http://www-lbit.iro.umontreal.ca/rnaml/current/rnaml.dtd. Within the next month or two, the RNAML output will contain the 'x, y' co-ordinates that correspond to the structure plots produced by the 'sir_graph' program, when they are available. The next year or two should see the incorporation of important, novel applications that are currently being developed.
New algorithms for the folding and hybridization of two separate strands of RNA or DNA have been created (41). Initial versions of the software are now being carefully reprogrammed and updated. We are investigating theoretical and experimental ways to improve the accuracy of predications. We are also beginning to consider dealing with the thermodynamics of hybridization of probes to nucleic acids immobilized on chips (42).
We are developing a new 'BLAST-like' algorithm for searching nucleic acid databases that is based on the computer science technique of hashing, for speed, but searches for regions of best complementarity between two sections of DNA or RNA. Scoring is based on nearest neighbor free energy parameters for RNA or DNA. An early version is now being used to search for putative binding sites for micro-RNAs (miRNAs) in databases of mRNAs, and to search for (undesirable) alternative binding sites for gene specific DNA probes.
The current user interface for the mfold web server is now several years old and would benefit from either partial or total rewriting. The submission of data through forms is still reasonable. A 'browse' option for uploading sequence files could easily be added. It would be useful to make it easier to enter constraint information. Even more valuable would be a preprocessor that would check the constraint information for consistency and reasonableness. Up to now, many constrained foldings have failed because of minor errors in constraints.
The output pages could benefit substantially by being redesigned. As a first step, the relatively new 'sir_graph' program should replace the older naview program for creating secondary structure plots. It would be desirable to create an interactive structure drawing feature. Such a feature already exists in the stand alone version of the 'sir_graph' program. We are fortunate here at RPI to have the cutting edge Academy of Electronic Media; a group that might collaborate with us in creating a really first rate user interface for the web server.
Authors who make use of the mfold web server should cite this article as a general reference and should also include the URL to the entrance page, http://www.bioinfo.rpi.edu/applications/mfold. The web server pages will list additional articles for citation that relate to the free energy parameters that are used and the underlying software.
Supplementary Material is available at NAR Online and can also be found at MFOLDROOT/www-NAR03/supp.
This work was supported, in part, by grant #GM54250 from the National Institutes of Health. I thank Gerald Johns for hardware and systems support in the early days of the server at Washington University. All of the graphics applications (except for naview) were written by Darrin Stewart, who also wrote the 'cgi' scripts for interactive viewing of plots. I thank Art Sanderson (Vice President of Research at RPI), for connecting me with the Academy of Electronic Media and for supporting this project; Bill Shumway, for initiating and facilitating interactions with IBM Research; and Alex Yu, who has done so much work in assembling the hardware, organizing the web server layout, porting applications and in keeping the server running day in and day out. Finally, I thank IBM Research for the SUR grant that gave us a thirty-fold increase in computer power for housing this valuable resource.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text:
The 73rd Hunger Games.Effie mentioned that both tributes from District 12 ate like savages and had no sense of etiquette or manners. This angered Katniss, as both were from the Seam and therefore they had never had a decent meal in their lives. Katniss mentions that Caesar's hair and make-up color was crimson and appeared to be bleeding.
In the film, Ceaser Flickerman and Claudius Templesmith watch a rerun of these games. Ceaser comments that's the ruined city arena was one of his "favorites." These games were supposedly won by a dark-skinned tribute, who in the film Katniss watched is seen killing Titus.
So this means that the victor of the 73rd Hunger Games was the male tribute from District 11, who killed Titus to win the Hunger Games.
This was Seneca Crane's second year as Head Gamemaker.


text:
State and county holiday today!
Confederate Memorial Day today is a state and county holiday—meaning Oconee government offices are closed, except the vital services. On this Confederate Memorial Day the musical group Confederate Attitude will perform southern rock music during a block party in Walhalla that begins at 5 o'clock. It's the start of a two-day Mayfest spring festival in Walhalla with arts, crafts and food booths on Main Street from 11 to 6. One of tomorrow's features is a parade of bicycles.


text:
This guy's name got removed from teh company website, when the rest of the team is still there.
He was a new hire.
Most Helpful Guy
Sounds like he got laid off to me.00|00|0
Wouldn't they remove all the material related to him if he got laid off? I mean, he was barely three months or so in, and there's still some stuff on him when he first got hired.
Plus it's listed on his linkedin


text:
This is a wonderful metal barn strap. It weighs just over 5 lbs. It measures approximately 30" long. The widest part of the strap is approximately 2 ½" wide.
The condition is fairly good. There are no breaks or cracks but the strap is bent. There is pitting and there is some old paint left on the surface.
This old barn strap would be a great gift for someone restoring an old barn or structure.
Specializing in primitives, porcelains, antiques, costume jewelry and unusual collectibles.
Thanks for visiting our shop. Due to a devastating house fire we are going to be a little slow getting orders shipped.


text:
These women's Adidas Adizero Boston Boost 5 Running Shoes are designed for racing and fast training. With boost™ in the midsole for an energy-filled, yet stable ride from the first step through the last mile. The durable, lightweight mesh upper keeps your feet cool. A portion of each pair sold goes to the Boston Runs As One fund.
- Weight: 190 g (size UK 5.5).
- boost™'s energy-returning properties keep every step charged with an endless supply of light, fast energy.
- Coolever mesh upper and lining for heat management and enhanced performance; Tirrenina synthetic suede overlays for added support.
- Extended TORSION® SYSTEM for energy return in the forefoot and natural integrity in the midfoot.
- Moulded responsive EVA sockliner for anatomical fit and great step-in comfort.
- Continental™ Rubber outsole for optimal grip in wet conditions.
Additional Information
|Model Number
|Manufacturer Colour
|White / Core Black / Shock Red
|Footwear Type
|Lightweight & Racers
|Shoe Collection
|Adidas Boost
|Spring 2016, Summer 2016


text:
Lady is a small purple tank engine who runs on the Magic Railroad. She has only appeared twice so far in the Thomas The Tank Engine. She first appeared in the film Thomas & The Magic Railroad and then later in Calling All Engines! however this was only a minor apperance. She has not appeared in any episode of the show until Season 21.
Allies: Thomas (boyfriend), Edward, Henry, Gordon, James, Percy, Toby, Duck, Donald, Douglas, Oliver, Emily (best friend), Molly, Rosie (cute friend), Rusty (Narrow Gauge diesel friend), Proteus (Narrow Gauge friend), Murdoch, Spencer, Dennis.
Enemies: Diesel, Arry, Bert, Diesel 10 (Worst Enemy/Kidnapper & Criminal), Splatter, Dodge, Spencer, Daisy, George, Bulgy, D261, Steam Engine, Dennis (formerly), Elizabeth, Cranky, Bill, Ben, S.C. Ruffey, Judge Judy the Diesel, D199, Diesel 20, Den, Dart, Smudger, Troublesome Trucks in general, Max, Monty, and all other evildoers of Sodor.
Well She is the Lost Engine in TATMR.
- When Lady is not on Sodor, she becomes faceless.
- Lady's theme is the instrumental of the opening credits of Thomas and the Magic Railroad.
- Gracie Jones - Shining Time Station
- Sailor Moon - TUGS, fan-made
- Bluenose, Isabel or Pugwash - Theodore Tugboat
- Princess Rosalina - Super Mario Bros.
- Blaze the Cat, Princess Sally Acorn, Cosmo the Seedrian, Vanilla the Rabbit, Amy Rose, Cream the Rabbit, Shade the Echidna, or Tikal the Echidna - Sonic the Hedgehog
- Lady the Traction Engine - Garfield and Friends, fan-made
- Piper Perfect - Wacky Races Forever Pilot
- Mindy - Animaniacs
- Nadine Flumberghast - Arthur
- Mindy - SpongeBob SquarePants
- Dawn - Total Drama
- Nokola - Boinicle School/Bionicle
- Elsa the Snow Queen - Frozen, Lady and Elsa are both magical
- Cleopatra "Dirtbike" Funnie - Doug
- Cararise's Tricicle "Loly" or Fairy Trikzie - Carasire Aurely's Avdentures
- Quarky the Lab Girl - Kablam!/Action League Now!
- Harmony Angel - Captain Scarlet and The Mysterons
- Marina - Vehicles
- Damy - Titielenaders, Both end with "y"
- Daisy-Head Mayzie - Dr. Seuss
- Kathy Copter - Cars
- The Teeth Fairy - Claude & Cia's World
- Maggie Simpson - The Simpsons
- Olivia Fuller - Family Guy
- Heidi Turner - South Park
- Luanne Platter - King of the Hill
- Twilight Sparkle, Princess Cadence, Rarity or Fluttershy (Rainbow Dash in BrittalCroftFan Version) - My Little Pony: Friendship is Magic
- Ashley - WarioWare (T,J&H Version)
- Penny Ling - Littlest Pet Shop (2012)
- Carmen the Whale - Rayman
- Jasmine Fenton - Danny Phantom
- Skyler - Good Luck Charlie
- Muk Muk - Fanboy and Chum Chum
- Ottoline Otter - Rupert
- Lil - Rugrats
- Numbuh 10 - Codename: Kids Next Door
- Dinah - Alice in Wonderland
- Mrs. Noah - Noddy's Toyland Adventures
- Rosie Grape or Esther - Veggietales
- Cresselia - Pokemon
- Lumpy Space Princess - Adventure Time
- Amber - Scooby Doo
- Abby Cadabby - Sesame Street
- Dottie - Planes
- Venus Di Milo (they both have magic powers), Catlin (wear/are pink), or Kris Mu - Teenage Mutant Ninja Turtles
- Star Strike - Skylanders
- Sam Sparks - Cloudy With a Chance of Meatballs
- Gertie Growlerstein - Henry Hugglemonster
- Mothra - Godzilla, both are kind and magical
- Egyptian Woman - Mr. Peabody & Sherman (2014)
- Besa the Black-Bee - Maya the Bee
- Coral - My Friend Rabbit
Voice Actors
- Jules de Jongh (UK/US)


text: congrats Minnesota you now have a full staff of former cowboys coaches, two have never really achieved any high level of anything in Edwards and zimmer. I cant tell you how happy I am that zimmer and turner in no way are coming here. next year when the garrett goose is cooked I hope we have a whole new direction with all new staff and hopefully some front office people. Look around the league and think of the units that are always good and play at a high level no matter what...those are the places I want to find my coaches. What happened in San Diego this year is a pretty large negative for Norv Turner IMO, its no different than if we had hired say Pete Carmichael this year or ken whisenhut and made the playoffs at 9-7. People would be saying see how garrett held them back. Pretty Clear norv had issues in san diego. Again, I am so glad none of these names are coming here in the future.


text:
Question: Describe a database that would be useful to a local
Describe a database that would be useful to a local pizza restaurant.
View Solution:
View Solution:
Answer to relevant Questions1. Based on the output from their data search, the Fulkersons have a number of variables that can be used to assess the market potential for furniture stores in each market. What are these variables? 2. What is wrong with ...What should be included in a report that summarizes the findings of a focus group? What is physiological measurement? Give examples of two techniques. Describe what a focus group company facility looks like and how a focus group would take place in one. With a telephone survey, when a potential respondent refuses to take part or is found to have changed his or her telephone number or moved away, it is customary to simply try another prospect until a completion is secured. ...
Post your question


text:
LONDON – As the third anniversary of the start of Syria's civil war approaches, there is a race against time to deliver a groundbreaking education project to the conflict's hardest-hit victims – hundreds of thousands of child refugees.
A shocking three million Syrian children have now been displaced. More than one million of them have fled Syria and are languishing in camps in neighboring countries, particularly Lebanon, Jordan, and Turkey. These children are now suffering a third winter away from their homes, schools, and friends. Many are separated from their families, and thousands more join the ranks of displaced persons every day in what is becoming the largest humanitarian catastrophe of our time.
But a pathbreaking initiative in Lebanon, involving teachers, aid agencies, and education charities has opened a small window of hope. Amid the chaos of camps, makeshift huts, and destitution, the fight for an important new principle of international aid has begun: even in times of conflict, children must have access to education.
A century and a half ago, the Red Cross established the norm that health care could – and should – be provided even in conflict zones. This principle was carried forward by groups like Médicins sans Frontières, whose doctors have risked their lives for the last four decades to deliver medical care to the world's most dangerous places.


text:
- Experimentalist
- Experimentalist Ex*per`i*men"tal*ist, n.
1. One who makes experiments, especially one who likes to
experiment; an experimenter. --Whaterly.
[1913 Webster]
2. One who relies primarily on experimentation and the evidence of one's own senses; an empiricist; -- contrasted with {theoretician} or {dogmatist}. [PJC]
The Collaborative International Dictionary of English. 2000.


text:
All links on this page are taken from public sources such as search engines (yahoo.com, google.com, bing.com). However, if you think any data on this page violates your copyright, please send an message from "Contact webmaster" page and the links and descriptions of full page will be removed.
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text:
Frances Fuller
In Lebanon, a country racked by war, the Fullers are house-hunting, because they have a job to do--- publishing Christian literature in the Arabic language. The house they find is mostly a heap of rocks, Frances thinks, but Wayne begins turning it into home. Meanwhile the Syrian occupation, an Israeli invasion, the bombing of the American Marines, artillery battles and kidnappings impact their lives. Their sense of purpose and relationships with courageous and resilient friends strengthen their resolve, while the little rock house becomes a haven, and powerful external forces threaten their mission.
In Borrowed Houses has received four awards.
Frances Fuller is also the author of:
• The Chameleon's Wedding Day (short stories), Lion Publishing, Oxford, England.
• Portraits of Women in the Bible (8 articles), New Lion Handbook to the Bible and Zondervan Handbook to the Bible.
• Pictures of Jesus (in Arabic), Baptist Publications, Lebanon.
• How to Read the Bible (in Arabic) Baptist Publications, Lebanon.
• The Spiritual Foundations of Freedom (in Arabic), Baptist Publications, Lebanon.
• Articles in: The Commission magazine, Interlit (David C Cook Foundation) and in Baptist state papers all over the U.S.
Each year 50 Great Writers You Should Be Reading are chosen by the vote of internet radio listeners. From these 50 voters then choose a Grand Prize winner, and Frances Fuller was the Grand Prize winner in 2015.
The Illumination Book Awards is an international contest for the purpose of "Shining a light on exemplary Christian books." Winners are decided by panels of judges. In Borrowed Houses received the bronze medal for memoir in 2014.
Northern California Publishers and Authors annually gives awards for literature produced by residents of the area. In 2015 In Borrowed Houses received two prizes: Best Non-fiction and Best Cover.
More on Frances Fuller and his/her work here


text:
SEVENDUST - Decay - Guitar Tutorial MP3
Guitar lesson for the song "Decay"
CARNIFEX - Condemned To Decay (GUITAR LESSON / DIE WITHOUT HOPE) MP3
Guitar lesson with Jordan Lockrey and Cory Arford of Carnifex. Order at: http://smarturl.it/CRNFX-DWH-NB Subscribe to Nuclear Blast: http://bit.ly/subs-nb-yt ...
Overkill - The Years Of Decay - Full HD Guitar Cover 1920x1080p MP3
One of the best songs of 89/90's is out now! Enjoy the only one Full HD Years Of Decay guitar cover like you've never seen and heard before!
Sirenia - The Path To Decay (guitar cover) MP3
Sevendust - Decay (Guitar Cover) MP3
Tab: http://tabs.ultimate-guitar.com/s/sevendust/decay_tab.htm A very early run through of the brand new Sevendust single, "Decay", off their forthcoming album, ...
Majesty And Decay Guitar Tutorial by Immolation MP3
Here's a guitar lesson on how to play Majesty and Decay by Immolation. I'm using an Ibanez RG370 DX with active EMG pickups. The amp is a Line 6 Spider 75.
Tremonti - Decay (guitar cover) MP3
Make sure to "like" by facebook page! http://www.facebook.com/justinvt.fans Guitar only track: http://www.mediafire.com/?4fd3jzostb017f8 Tuning is Drop B. That ...
Chronosphere - Brutal Decay Guitar Cover MP3
the GazettE -Before I Decay- Guitar cover (Uruha's part) MP3
New cover again ^^ I am not a pro so it doesn't sound perfect but I hope you still enjoy it :D I do not own the song.
Immolation - Majesty and Decay (guitar cover) MP3
Majesty and Deacy, the title track of the 8th studio album produced by Immolation!! One of the best Death Metal bands ever.....enjoy! guitar tuning : Standard C ...
The Devil Wears Prada - Forever Decay (Guitar Cover) HD MP3
This is me playing "Forever Decay" by The Devil Wears Prada. Sorry for some mistakes. I hope you enjoy it. Please feel free to comment. PRS SE Torero Zoom ...
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Rafive - Minute of Decay (Marilyn Manson Guitar Cover) MP3
Rafive - Minute of Decay (Marilyn Manson Guitar Cover) Sorry for mistakes. https://www.facebook.com/rafiveofficial.
Deron Miller CKY guitar riffs MP3
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Here is my cover of Decay by Mark Tremonti. All song rights belong to Mark Tremonti and Fret12. This video is for instructional and entertainment purposes only.
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text:
Beneath Hill 60
Release Date: 11 October 2010 | Certificate: Suitable for 15 years and over
Featuring: Harrison Gilbertson, Gyton Grantley and Steve Le Marquand
£4.49 includes FREE UK Postage (other delivery options available)
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BENEATH HILL 60 tells the extraordinary true story of Oliver Woodward, the legendary Australian metal scientist. In 1916, Woodward faced the most difficult decision, ultimately having to separate from his new young love for the deadly carnage of the Western Front. On treacherous territory, behind the German enemy lines, Woodward and his secret platoon of Australian tunnelers face a suicidal battle to defend a leaking, tunnel system. A tunnel packed with enough high explosives to change the course of the War...
Commentary, Trailer, Featurette
Release Date: 11 October 2010
Certificate: 15 - Suitable for Persons Aged 15 or Over
Actors: Harrison Gilbertson, Gyton Grantley, Steve Le Marquand, Jacqueline McKenzie, Alan Dukes, Brendan Cowell and Alex Thompson
Directed by: Jeremy Sims
Region 2
Aspect Ratio: 16:9 Anamorphic Wide Screen
Sound: Dolby Digital\Dolby Digital 5.1 Surround
Running Time: 100 minutes
Production Year: 2010
Main Language: English
Subtitles: English
For more information and our Delivery Policy see here.
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Catalogue No: MP1075D


text:
In Washington, the debate over how to address inequality - whether of income or opportunity - rages, as scholars, policy wonks and politicians often far-removed from these problems wrangle over whose solution is best and whose will do harm.
For Robert Woodson these detached academic notions are part of the problem.
For more than 30 years, Woodson, a founder of the Center for Neighborhood Enterprise and a product of the civil rights movement, has been challenging the way the U.S. addresses communities in need, with notable success.
Empowering the marginalized is a challenge that conservatives should accept, particularly since Woodson's approach relies heavily on conservative principles.
Since founding the center in 1981, Woodson has applied a theory of "social entrepreneurship" to tackling the most vexing issues in cities such as Milwaukee and Chicago.
"The principles of the free market economy should be applied in the social economy," Woodson told me.
His organization goes into inner cities and struggling neighborhoods, finds people and programs that are providing effective localized solutions, and helps provide them with the resources and training they need to "grow along a continuum."
He's a kind of social venture capitalist, although his nonprofit is always in search of capital.
The pursuit of virtue is an essential element of the battle against societal problems that Woodson believes can only be addressed through individual transformation, a lesson he says is illustrated by Christ's teachings.
His approach to lifting people out of poverty and violence differs from that typically supported by liberals today, which has a penchant for ever-expanding, top-down government programs that cause "a lot of injury with a helping hand."
Liberal policies, he told me, can be "equally injurious, indifferent and exploitative," because they don't empower the disadvantaged.
But he reserves some criticism for conservatives, who he says have made a "grievance industry" out of talking about what they're against instead of what they're for when it comes to making a difference for the truly struggling.
"Conservatives," he said, "think if you just make a logical argument" people will get on board. But arguments fail to resonate with desperate people.
While Woodson largely seems to believe that conservative concepts make for sound public policy, he sees demonstrating how these beliefs make for a better life as the missing element of contemporary Republican politics.
Conservatives who recognize the wisdom of this approach must be resolute, if only because after so many years Woodson remains optimistic.
"We only need one person who gets personally committed to change the party," he says, and he may have found that person in Rep. Paul Ryan.
For some time now this dynamic duo has been traveling to low-income, violence-plagued communities on a listening tour to identify better ways to help the poor and marginalized of society.
Ryan, as House Budget Committee chairman, is uniquely positioned to use this personal experience - which Woodson believes to be crucial - to advance a conservative anti-poverty agenda.
The fight against income inequality has been named by President Barack Obama and congressional Democrats as the issue they will run on in 2014. So voters will be hearing a lot about it this year.
But when voters hear liberals accuse conservatives of being cold-hearted, they shouldn't assume that liberal approaches to lifting up the marginalized are good ones.
Thanks to people like Woodson, conservatives can counter with detailed ideas of their own - which have the added benefit that they work.
Cynthia M. Allen is a columnist for the Fort Worth Star-Telegram. Email her at firstname.lastname@example.com.
Distributed by MCT Information Services


text: Skate or Swim 200px Season: 3 Production code: {{{production}}} Broadcast number: {{{broadcast}}} Broadcast Information Disney XD US Premiere: March 5, 2012 International Debut: to be announcedSee more... Retrieved from "http://zekeandluther.wikia.com/wiki/Skate_or_Swim?oldid=5462" Ad blocker interference detected! Wikia is a free-to-use site that makes money from advertising. We have a modified experience for viewers using ad blockers Wikia is not accessible if you've made further modifications. Remove the custom ad blocker rule(s) and the page will load as expected. Categories: Pages with broken file links Episodes Season 3 episodes Add category Cancel Save


text:
Inheritance part 3
I have inherited to celebrate the holiday of Eid.
The story about my inheritance is that we celebrate Eid every year and. We have to get up in the morning to go to the mosque to pray and then we just celebrate the whole day.
That it is importance to all Muslim people and we all love to celebrate this holiday because this is the only holiday we celebrate.
Eid means to so much to us and that day we don't do any work . We just have fun I guess.
I think I don't know that much about Eid but I want to learn more about it.


text:
Committed to bringing electronic engineers the newest technologies first, leading multi-channel electronics distributor Newark, part of the global Premier Farnell Group, announces that Cree will be one of 31 top suppliers featured at the "Energy Efficiency & Lighting" global technical learning and networking event, held virtually at the Live EDGE EcoSphere on December 11, 2008 from 8am to midnight EST. Engineers and engineering students are invited learn about the same Cree technologies that were showcased at the Beijing Games at the online event.
Approximately 496,000 Cree XLamp® LEDs in red, green and blue were used last summer to illuminate the exterior of the Water Cube in Beijing. Modules of 8-16 LEDS were integrated into the steel structure of all four walls and the roof. Cree LEDs were also used on the exterior of the Bird's Nest, and helped to add drama to the opening and closing ceremonies. In addition to indoor and outdoor general illumination, Cree LEDs are used in portable lighting, retail display and electronic signage. A Cree design guide and its newest technology products are available for same-day shipping at www.newark.com/cree.
Live EDGE — Electronic Design for the Global Environment — is the name of Premier Farnell's $100K design challenge. The distributor developed the EcoSphere community in support of its international competition and the global student and professional engineering community. Throughout the Dec. 11th event, there will be presentations from suppliers followed by live chat discussions. Attendees will be able to ask questions, download technical information and network with colleagues. Free registration and a complete schedule of the December 11th event is available at www.live-edge.com/ecosphere. Two additional EcoSphere events are scheduled: "Communications" in February '09 and the Live EDGE Awards Ceremony and Technical Conference in April '09.
About Newark
Newark, part of the global Premier Farnell Group, and a leading multi-channel, high-service electronics distributor supporting millions of engineers and purchasing professionals across the Americas, is known for offering the broadest selection of components and test equipment from 440+ top manufacturers for design engineer and maintenance professionals. With multiple sales channels, customers do business with Newark through contact centers, local branch network, field sales force and a world-class website.


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Previous Consultations
09.09.16Department's submission to Central Bank's review of the macro prudential residential mortgage lending measures
The Payment Accounts Directive
The Minister for Finance, Michael Noonan T.D. today opened a public consultation on the Financial Institutions Levy.
29.01.164th EU Anti-Money Laundering Directive and Funds Transfer Regulation - Public Consultation on Member State discretions
The Department of Finance and the Department of Justice and Equality invite interested parties to make submissions in relation to member state discretions contained in the Directive (EU) 2015/849 on the Prevention of the use of the Financial System for the Purposes of Money Laundering or Te
Public Consultation on the Multilateral Interchange Fee regulation 2015
- 1. AvantCard.pdf
- 2. Banking _ Payment Federation Ireland.pdf
- 3. Barry Group.pdf
- 4. Chambers Ireland.pdf
- 5. Coxs Cash _ Carry.pdf
- 6. CSNA.pdf
- 7. GFK Investments.pdf
- 8. Retail Ireland.pdf
- 9. AEX Consulting.pdf
- 10. MasterCard.pdf
- 11. Murphys Cash _ Carry.pdf
- 12. Musgraves.pdf
- 13. Padraic Tuffy Limited.pdf
- 14. Diners Club.pdf
- 15. Retail Excellence Ireland.pdf
- 16. RGDATA.pdf
- 17. SFA.pdf
- 18. Stone House.pdf
- 19. SuperValu Carrickmacross.pdf
- 20. VISA.pdf
03.07.15Joint Public Consultation Paper Department of Finance/Central Bank of Ireland Funding the Cost of Financial Regulation
Interested parties are asked to comment on the public consultation by 25 September 2015
Public Consultation on Transposition of the Deposit Guarantee Directive
The Minister for Finance, Michael Noonan TD, invites interested parties to make submissions in relation to the transposition of the Deposit Guarantee Directive.
20.05.15Public Consultation on the Regulation (EU) 2015/751 on Interchange Fees for Card-Based Payment Transactions
Public Consultation on Interchange Fees for Card-Based Payment Transactions
This Consultation period has now been extended to Wednesday, 10 June 2015".
The Minister for Finance, Michael Noonan TD, invites interested parties to make submissions in relation to the potential impact for Ireland arising from the Interchange Fee Regulation.
The consultation period will run to 5pm, 21 April.


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Notebook: Teams trade headaches
Track star Demps dealt for Blount
Credit: Unknown
FOXBORO — In recent days, it became clear Jeff Demps wouldn't be returning to the Patriots and LeGarrette Blount was out with the Tampa Bay Buccaneers. Naturally, the teams decided to swap their unwanted running backs.
The Pats yesterday sent Demps and a seventh-round pick (No. 229 overall) to the Bucs for Blount after fielding calls from several teams that were interested in acquiring the Olympic silver medalist. Demps informed the Patriots this offseason that he was going to pursue his track and field career, and he has already run some events.
|The article you requested has been archived
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Clint Brown
- Overview
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|1990s 2000s
c. 1963, Iota, Louisiana, USA. One of the leading praise and worship leaders in the USA, Brown is also a prolific recording artist and songwriter. After leaving school he initially worked as a mechanic on gas pipelines before taking to the road as a travelling musician and singer. He began his career in the church in his native Louisiana, but had soon relocated to Columbus, Ohio, to become music minister at Rod Parsleys World Harvest Church. In 1993 he moved to Orlando, where, with a rapidly expanding congregation, he launched the Faithworld Church. Over time the church expanded to a paying congregation of over 6, 000 members, although Browns preaching of prosperity gospel raised a few eyebrows among other churches. By the mid-00s Brown was a rich man, but his finances were made public when a former congregation member raised a lawsuit to reclaim money, and his wife of over 20 years filed for divorce.
Brown began a concurrent...
- MatchClint Brown
- 42%Monique Walker
- 38%Desmond Pringle
- 35%Lejuene Thompson
- 33%Phil Tarver


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Add subaccount and remove subaccountsAsked by CharlesCannon on December 10, 2015 at 10:46 AM
JotForm Support
Thank you for contacting us.
Yes it is possible to add are removed Sub Account users.
Inside of this guide you can find how to add and share forms with a Sub Account User:
If you want to remove user go to your account, select Users tab. Select user which you want to remove click on Edit option and then click on Delete User button.
Hope this will help. Let us know if you need further assistance.


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Separate names with a comma.
no one wants to see this shit from a brand new member before they know you.
what ever
Why look in someone's album if you don't want to see stuff? Better here than in the threads. Smally9666, thanks for sharing.
thanks for your support draggon fire
i will suck those balls!!!
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[URL=http://www.sexualforums.com/gallery/photos/351/]351[/URL] by [URL=http://www.sexualforums.com/gallery/authors/smally9666.35120/]smally9666[/URL] on SexualForums


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With First Tutors: Music you can search for a Port Isaac keyboard teacher. Keyboard lessons are the perfect method to start an entertaining new hobby, acquire a new talent and attain confidence with keyboard. Use our site to find Port Isaac keyboard teachers and select which matches your requirements so you can start your keyboard tuition in Port Isaac straight away!
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Canon MG5751 Driver Download for OS Windows, Linux and Mac – PIXMA MG5700 Series Hassle-free printing and scanning with wise devices and the cloud. Rejoice producing stunning illustrations or photos and files using this type of adaptable, user-friendly, 5-ink All-In-One.Effortlessly link, print, scan and duplicate in the home applying this inexpensive, top quality 5-ink All-In-One Easily connect, print, copy and scan in your own home making use of this reasonably priced Wi-Fi All-In-One. Shots are going to be filled with high amounts of depth, deeper blacks and much more vivid reds – thanks to 5 single inks, Canon's Fantastic technological innovation and four,800dpi print resolution. ISO ESAT speeds of twelve.6 ipm mono and nine.0 ipm color produce an attractive 10 x15 cm borderless print in close to forty one seconds.
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When a guy acts really sweet to a girl in person but doesn't answer phone calls or text messages...what does it mean?
Most Helpful Guy
He's probably nervous or does not know what to say. If you have his address you could aways go to see him in person.
Plus there is a school of thought that says a man should try to not act too interested. That while women say they want a ton of attention...that when it comes down to it... men who they have to work to get the attention of... get more action. You would see this written of online as the "bad boy" vs "nice guy" debate.00|00|0
what do you mean guys should act too interested? how would you know if he's following this "rule" or just isn't interested? and do the majority of guys follow this? thanks :)
I kinda get this. I think he's been hurt before and it was fairly recent. Also, he's incredibly nice and sweet in my face...just bad about the phone thing. That was more my question...wouldn't a guy avoid me if he wasn't interested?


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You will require this registration if you intend to operate a thoroughbred racing club and hold race meetings in the Australian Capital Territory (ACT). A race club promotes, conducts or controls a horse racing meeting or meetings. All race meetings will need to be approved by the Agency and will be kept on a register.
Please be aware that your registration may be subject to terms and conditions and will need to comply with the relevant rules.
Please consult the Contact Officer for more information.
A licence defines the need to obtain recognition / certification and registration to undertake a certain business activity.
Please consult with the Contact Officer for information on any eligibility requirements.
Please consult the Contact Officer for information on fees to be submitted.
Registration of Clubs and Meetings - Please consult the Contact Officer for information on forms to be submitted.
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For all its beauty, honesty and emotion, country music also has a reputation as "white people music." It's an unfortunate label that only alienates fans and keeps the genre stuck in trite stereotypes and cliches.
But it's not entirely untrue, thanks in large part to skittish label executives who are afraid to think outside of the box they built for their own genre. Singer Carl Ray said it best when he told The Guardian, "'I perform in places where I'm the only African American and I've never had a bad experience. The problem is not with the fans, it's with the executives – they're trying to go with what's worked before, and they're trying to keep their job."
That was 10 years ago.
In reality, country music owes its deepest roots to black musicians and artists — like the ones who taught country pioneer Jimmie Rogers everything he knows about music. There's a whole crop of black country artists on the rise. With any luck artists like Mickey Guyton, who has been the favorite of both fans and critics since she stepped on the scene, will be recognized for their talent before their skin color.
Here are six black country musicians who changed country music.
DeFord Bailey
DeFord Bailey was a pioneer not only for black country musicians, but all country musicians. A world-class harmonica player, Bailey has the distinct honor of being the first musician to be introduced on the Grand Ole Opry. Yes, the first performer on the show was a black man. Opry founder George Hay made the announcement live on air on Dec. 10, 1927, when the show officially adopted its name, changing it from WSM Barn Dance, which it had used since it started in 1925.
Bailey would go on to play the Opry regularly as well as tour with greats like Bill Monroe and Roy Acuff through 1941. Unfortunately, a conflict with his recording rights essentially ended his career that year. A licensing conflict between two organizations made it so he could not perform his most well-known songs on air, and soon Bailey was relegated to shining shoes and renting rooms for a living.
A rare appearance in 1974 launched the Opry's "Old Timers' Show," and Bailey received one of country music's greatest honors when he was inducted into the Country Music Hall of Fame in 2005 — 23 years after he passed away.
Charley Pride
One of our living legends of country music, Charley Pride embodies everything there is to love about country. With talent matched only by his resiliency and character, Pride rose to fame at the height of racial tension in America and eventually earned 39 No. 1 singles, selling over 70 million albums. The only artist to outsell Pride on his label was Elvis Presley.
But it's not just about sales, Grammy's, Entertainer of the Year awards or "first black country artist" moments, though Pride certainly has his share of those. What makes Pride such a huge influence on country music is the way he changed perceptions of what it meant to be "country."
Most listeners and radio programmers didn't realize they were listening to a black man in the 60s when Pride first hit the radio. That was partially due to the absence of a picture on his promotional singles.
When they did learn, it didn't matter. Pride's smooth voice helped ease the tensions of the working class struggles he sang about and resonated with, and his history as a hard-working former baseball player who got his start singing before the games was endearing. He trampled the prejudices of some of his biggest fans simply by being who he is.
To this day, Pride insists that when it comes to music his color is not important. But he showed just how colorblind music really is at a time when the country, and country music, needed it most.
Pride recently kicked off his 50th anniversary tour, and you can find dates here.
Tina Turner
She may not be known traditionally as a country artist, but Tennessee native and American icon Tina Turner's stint in country music was huge both for her and for future generations of artists. She wasn't the first pop/R&B artist to go country (there's another coming up in the list), but she chose to introduce herself to the world as a country artist to show her appreciation for the genre.
In 1975, only a year before leaving her abusive husband Ike Turner, Tina stepped out on her own to release her first ever solo record. A collection of her take on some of her favorite country songs, Tina Turns the Country On! earned her a Grammy nominations (albeit in the R&B category, thanks to the awards' old way of categorizing music).
And while she didn't gain breakthrough solo success until the 80s, Tina's stint as a black country artist inspired a whole world of musicians to cross into country. Her booming personality and vocal delivery also inspired a new wave of big-voiced country women like Reba McEntire to take control of the stage, instead of sticking to the reserved demeanor seen in most country artists at the time.
To this day, her hometown celebrates her country roots with its Tina Turner Heritage Days every year.
Ray Charles
Ray Charles is the rare artist whose music completely eclipses genre boundaries. And while, like Tina Turner, he's not known traditionally as a country artist, Charles stepped into the country spotlight several times and left a lasting impact.
For starters, one of Charles' first gigs as a musician in the 40s was playing with a country band called The Florida Playboys. He first began recording country songs in the late 50s, and in 1962 he released Modern Sounds in Country and Western Music.
The album was one of many of Charles' homages to his love for country music (he released another volume and several country singles throughout his career), and is widely considered to be Charles' greatest record and one of the most important country records of all time.
Charles' signature arrangements, complete with spunky piano licks and big band punctuation, helped introduce country music to an entirely new generation that had never heard some of the genre's biggest hits before. Thanks to Ray Charles, country music's popularity exploded. He paved the way for fledgling artists like Merle Haggard to become bona fide American superstars.
Cowboy Troy
Some of today's best-selling country acts owe the genesis of their biggest hits to Cowboy Troy. How so? Well, he was doing the whole "hick-hop" thing years before acts like Jason Aldean ("Dirt Road Anthem"), Luke Bryan ("That's My Kind of Night") and Florida Georgia Line ("This Is How We Roll") cashed in on it.
Sure, it may not be the kind of "change" a lot of traditional country fans were hoping for, but Cowboy Troy's mark on country music is undeniably huge. What separates Troy Coleman III from the other acts is that he was always making music that fused country twang with rock music and hip hop lyric schemes. Unlike artists who saw their stars rise with a hick-hop hit or two, it was never just a fad for Cowboy Troy, who started in 2001.
The conservative Texan grew up in Dallas and graduated from The University of Texas at Austin. He had success with his first major label record in 2005, which peaked at No. 2 on the album charts. From there, Troy ultimately became a fixture on television, co-hosting and appearing on a number of shows, from Nashville Star to Dancing With The Stars.
He's one of the original members of Big & Rich's "MuzikMafia," which also saw success prior to the 2010s. But Troy's influence on the scene can ultimately be heard in the hits that launched other (notably white) artists to their sold-out World Tour status.
Darius Rucker
Darius Rucker is, in many ways, the new torchbearer for country artists who don't fall in line with the expected "white southerner" trope. Though his solo career as a country artist is still less than a decade old, Rucker's influence and success already has him pinned as the "next" Charley Pride — and not just because of his skin color.
As a founding member and primary songwriter for soft rock band Hootie & The Blowfish (contrary to popular belief, the name was a combination of nicknames and Rucker was not "Hootie"), Rucker co-wrote the 16th best-selling album of all time in Cracked Rear View. He always described his sound as more country-leaning and introduces the classic hit "Let Her Cry" as the first country song he ever wrote.
When Rucker first went solo, execs tried to get him to release an R&B record, which was met with minimal fanfare. However, when Rucker got his chance to make the music he felt most at home making, 2008's Learn To Live was born. The album led to three No. 1 country hits, including "Don't Think I Don't Think About It" and "Alright."
It also laid the groundwork for his second record, Charleston, SC 1966. Named in homage to one of his heroes Radney Foster (whose Del Rio, TX 1959 is widely considered one of the greatest country records of all time), the record cemented Rucker as a mainstay in country music. He'd eventually go on to record one of the most-played country songs ever, his version of "Wagon Wheel."
All the while, Rucker has been received as a masterful songwriter with a genuine honesty in his delivery and a sound that is both uniquely his own and respectful of the deepest country traditions. Rucker is not simply an influential black country artist, he's one of the best songwriting artists in modern country music.
And while Rucker downplays the role of color in his career (like Pride), his success is important. As long as there are gatekeepers in country music, there will be barriers to truly talented artists getting the support they deserve. And no matter the record-setting careers of folks like Pride and Rucker, suits are nervous of breaking from stereotypes.
But fans want to hear great country music, regardless of color. The backbone of country music was built and is strengthened by artists both black and white. These pioneers proved the effect they can have on the critical and commercial success of country music, and little by little are reducing notion that country music is anything other than the music of all people.


text:
Shakespeare said that "all the world's a stage," mainly to explain why he faked his own death so many times. Live it up with this GrouponLIve deal.
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Unbroken Circle
Taking place in 1970s Galveston, Unbroken Circle delves into a family's shuttered secrets and strained relations as the estranged clan returns home for the patriarch's funeral. Co-starring Eve Plumb of The Brady Brunch fame and deftly mixing high pathos with sardonic humor, the show explores the ways a family's past can continue to affect its present, and the dangers of storing skeletons in the closet instead of using a sarcophagus like a normal person.


text:
The making of the Hobbit has gone through more twists and turns than our furry-footed little friend maneuvered on his trip to Mordor.
Now, back in the hands of master LOTR film maker Peter Jackson fans are once again re-energized. But the recent announcement that the movie would be split into three parts not two has caused some to scratch their heads, wondering if this is an artistic decision or another shameless money-grab to milk the release and DVD profits at the expense of storytelling. Only time will tell, but that shouldn't stop fanbois from getting all sweaty and excited over this upcoming release.


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
How can I collect bank details through form?Asked by Kim on January 18, 2017 at 10:37 AM
Hi - we are an LA based company and run events around the world. We have vendors who lecture at these events and pay them for their services via Transferwise. We are looking for a secure way to collect bank account details for payment to these vendors. Can I do that on JotForm?
JotForm Support
Hello Kim,
Yes, you can do that with JotForm. The best and, actually, the only way for collecting payments through our forms is by using Payment integrations. List of available payment processors you can find here: https://www.jotform.com/help/323-Mastering-Payment-Form-Integrations-with-JotForm
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Thank you1
Hey Nick,
As these are payments to our vendors for services and they do not take
credit cards, we use Transferwise as it is the most cost efficient way to
send money to places like Jakarta.
If my form asks for bank account details, you are saying I will not be
flagged and the account shut down. We tried Wufoo and they shut us down for
JotForm Support
Unfortunately, it is a similar situation here. It is against our Terms of use to collect credit card and any paying information from our forms.
What could be the closest to what you're trying to achieve is to use our Purchase order application https://apps.jotform.com/app/purchase_order_app which will allow you to create a form with products to sell, but without collecting payment information. But, you will not be able to collect payment information.
Hope it helps.
If you have any further questions please let us know.
Thank you!


text:
Hey, this is Thebiguglyalien! Now, I have come up with an annoying blog post, so if you have the ability to read, please keep reading.
I, like many people on this wiki, am a fanon writer. Some fanons are great successes. Every user is a reader. Now, I would like to congratulate users with such talent. But not all fanons end up this way. No, some go unread. Sad, but true.
Now, I'm going to give you a POP QUIZ! >:D Feel free to answer these questions:
- Do you have an unread fanon? Though this is not an advertisement blog, you can still tell me what this fanon is.
- What makes you think nobody reads your fanon?
- How does this make you feel?
Was the above question too mushy for you?Skip this question.
- Why do you think nobody reads your fanon?
- Do you think this fanon was good? Or was it a bad idea to begin with?
- Are you still trying, or have you given up?
My Answers
Feel free to skip this part.
- Yes, this is what gave me the idea, to see who else shares this problem.
- There is no signs of anyone having read it within... months?
- I was hoping my fanon would be read, but I knew it would never be very popular.
- I don't know the answer to this one.
- It's creative enough. There are none like it (that I know of).
- I feel it's a waste of time to write it sometimes, so chapters come out occasionally, but not often.
Now, what do you have to say about your undiscovered fanon?