text:
Become a racer for the Unbounded team. Consisting of a crack team of lawles... more
Add to Cart
Become a racer for the Unbounded team. Consisting of a crack team of lawles... more
Become a racer for the Unbounded team. Consisting of a crack team of lawless racers commanded by a mysterious leader called Kara Shindo, your aim is to dominate the streets of Shatter Bay and increase your vastly expanding territory.
Get ready for full on high-octane action with Ridge Racer Unbound: Limited Edition. Blast your way through buildings, pillars, trucks well anything that dare stands in your way as you plough through your tarmac playground.
Challenge your friends online in multiplayer mode as you prepare to leave them choking on your dust
Ridge Racer Unbound: Limited Edition includes:
Five brand new paint jobs:
> PAC-MAN™ (Kinghawk GT)
> GALAGA™ (Hurricana CX)
> TEKKEN™ (Crimson TT)
> SOULCALIBUR™ (Wolfseye GT)
> ACE COMBAT™ (Sylvian Type 8)
Three Limited Edition Vehicles:
>The Ghoster -This low rider coupe with its electric blue finish isn't for cruising but for bruising.
> The Immortal - This red super sports beast isn't easy to tame, but it's worth the effort.
> The Road Wolf - Eat up the competition with this classic red coupe inspired by cars of the 60's.
Minimum System Requirements:
OS:Windows XP, Vista SP2 32-bit, 7. [Windows up to date and latest service pack installed]
Processor:Dual Core Athlon x2 2.6 GHz or Intel Equivalent
Memory:2 GB RAM
Graphics:512 MB RAM, ATI Radeon 4850 or higher, NVIDIA GeForce 8800 GT or higher
DirectX: 9.0c
Hard Drive:3 GB HD space
Sound:DirectX Compatible
Other Requirements: Broadband Internet connection
Get weekly secret deals, offers and freebies in our e-mail newsletter.
Join 32,902 other gamers and never miss another deal again!


text:
Apache Loses Market Shares to Microsofts IIS
Bad news for Apache: according to Netcraft statistics, the free Web Server lost market shares to Microsoft's Internet Information Services (IIS) Web Server in October.
In its survey in October, Netcraft saw Apache's market share drop to 47.73 percent while Microsoft IIS went to 37.13 percent. The gap of ten percent between Apache and IIS is the smallest that Netcraft has ever recorded since IIS was released in 1996. Apache dropped by 2.8 percent compared to September. The Web analysts identified the powerful growth of major blogging systems such as MySpace and Microsoft Live and the fact that 2.5 million domains were lost with Trouble-free.net as the reasons behind the losses.
SharepointI know quite a few of my customers got bought out by bigger companies and they shut down there Apache servers because the new company uses Microsoft Sharepoint which is IIS 7 based.
<A HREF="http://www.helixzone.net&qu...://www.helixzone.net
Issue 202/2017
Buy this issue as a PDF
LibreOffice 5.4 Released
Comes with improved support for Microsoft Office file formats.
Red Hat to Drop Support for Btrfs
The company is building their own storage solution called Stratis.
Reinvent your network with DevOps tools and techniques:
Opportunities and Risks: Containers for DevOps
Automated Builds Using CentOS 7 and Kickstart
Elasticsearch, Logstash, and Kibana – The ELK stack
Are you ready for DevOps? Try your luck with the beta version of Linux Professional Institute's new DevOps exam.
Kolab Now Integrates Collabora Online
Kolab Now subscribers now have access to Collabora Online.
Endless OS, a Distribution Without Internet
A new version of Endless OS has been released.
GitHub Announces Open Source Friday Program
They encourage people to take time out and work on open source projects on Friday.
System76 Announces Their Own Distro, Pop!_OS
Pop!_OS is based on Ubuntu and uses the Gnome stack.
Linus Torvalds Talks About His Motivation
He never tires of working on the Linux kernel.
Debian 9 Stretches Its Wings
The new release of Debian has a very strong focus on security.
Trojan Turns Raspberry Pi into a Cryptocurrency Mining Device
Two trojans in the wild are targeting Linux machines.

subset: commoncrawl


text:
Android 3.0 Honeycomb has been ported to the Nook Color. Most core functionality is still not available, but the delicious Honeycomb interface is very much in effect.
The B&N, out of the box, runs a customized version of Android 2.1 -- and it sports the same CPU and GPU as the Droid 2 and Droid X. Having said that, at $250 for a Nook Color, and once the bugs have been ironed out, it'll probably be the cheapest way to get your hands on a Honeycomb-powered tablet.
[tags]android 3.0.honeycomb,hacking,hackers,nook color,droid 2,droid x,porting,[/tags]


text:
The hotel makes its guests feel like home. It is well furnished and provides all the amenities required to ensure a comfortable stay. The hotel is a great budget accomodation for all travellers.
The hotel is located in Jalan Salemba, a main road leading from north Jakarta to south Jakarta passing central Jakarta. The location of Salemba is still within the area of Jakarta Pusat or central Jakarta.
All the hotel rooms feature air conditioning, desk, bathtub, shower, minibar, satellite or cable TV and fan. In addition to this, the hotel provides 24hr room service and laundry service/dry cleaning.
The hotel has an elevator, coffee shop, meeting facilities and a restaurant very near, just a few steps away.
Room Facilities:
Direct dial telephone
Satellite cable TV


text:
Cute busty blonde with round ass sucks two pipes at once...
2 vibrators at once
Blonde C solo masturbating at home with Give Me Pink
A sexy babe masturbating with two vibrators angry
Cute hunk swallows two rock solid pricks at once
Two cute grannies have fun with vibrators
Blonde Fucks Two Guys At Once
Blonde slut wants two cocks all at once
Sweet blonde babe fucks two babes at once
Hot blonde mommy swallows two cocks at once
Horny blonde shemale takes on two guys at once
Blonde slut gags on two fat fucks sticks at once
Bodacious blonde nympho has two hard cocks plowing her holes at once
Bodacious blonde cougar has two hung studs plowing her holes at once
Christen Courtney fuck with two big cocks at once
Stunning Timea having fun with two boys at once
stuffing his mouth with two cocks at once skillfully
Slutty blonde mom Vinnie has two hung guys pounding her holes at once
Stunning Time a having fun with two boys at once
Asian beauty is fond of relaxing with two dudes at once
Tiffany Limos having sex with two guys at once - giving one
BustyNetwork Brunette with Huge Natural Titties Fucks Two Guys at Once
Aiden masturbating with fingers at Give Me Pink
Ass Traffic Kitty plays with two cocks at once in pussy and
TrafficHolder.com - Buy and Sell Adult Traffic|Trafficshop.com - Let your business grow|Contact Us|Webmaster
All content, including images, text, graphics, video and audio content contained in the website is protected under the laws of copyright, owned or under license to company or its designees,and represents proprietary and valuable intellectual property.
Contact Webmaster 18 U.S.C. 2257 Record-Keeping Requirements Compliance Statement
Powered by Stream Rotator


text:
Photos are for members only!!
Peachmate is a 100% free sex site for adults looking for fun with no strings attached.
Escort ads, casual sex groups, personal ads, video chat and much more.
I'm a country boy who is a good lover looking for a women who is sweet and a great lover


text:
Hello Its Short code
All season are best to visit Bhutan in any time of year. Bhutan shows beautiful faces as in allseason. In fact, the four seasons (Winter, Spring, Summer & Autumn). The best/ideal time to visit Udaipur is from September to March. Get more on travel peak season of Udaipur.


text:
Adenosine 5′-cyclic diphosphoribose [cyclic ADP-ribose (cADPR)], a metabolite of NAD+ that promotes Ca2+ release from sea urchin egg homogenates and microsomal fractions, has been proposed to act as an endogenous agonist of Ca2+ release in sea urchin eggs. We describe experiments showing that a microsomal fraction isolated from Tetrapigus nyger sea urchin eggs displayed Ca2+-selective single channels with conductances of 155.0 ± 8.0 pS in asymmetric Cs+ solutions and 47.5 ± 1.1 pS in asymmetric Ca2+ solutions. These channels were sensitive to stimulation by Ca2+, ATP, and caffeine, but not inositol 1,4,5-trisphosphate, and were inhibited by ruthenium red. The channels were also activated by cADP-ribose in a Ca2+-dependent fashion. Calmodulin and Mg2+, but not heparin, modulated channel activity in the presence of cADP-ribose. We propose that these Ca2+ channels constitute the intracellular Ca2+-induced Ca2+ release pathway that is activated by cADP-ribose in sea urchin eggs.
- calcium release channels
- ryanodine receptors
- intracellular calcium
- oocytes
- endoplasmic reticulum
- calmodulin
- intracellular channels
release of Ca2+from intracellular stores has a central role in egg development after fertilization (19, 30). Consequently, the characterization of the physiological agonists that elicit Ca2+ release in oocytes is a highly relevant topic that is the subject of active investigation. In the case of sea urchin eggs, three independent Ca2+ release pathways, sensitive to inositol 1,4,5-trisphosphate (IP3), ryanodine, or nicotinic acid adenine dinucleotide phosphate, have been described (5, 7, 12,21). In addition, adenosine 5′-cyclic diphosphoribose (cADPR) promotes Ca2+ release in sea urchin egg homogenates and microsomes (14, 23, 25). Agents that inhibit Ca2+ release through the ryanodine receptors-Ca2+ release channels (RyR-channels) of sarcoplasmic reticulum (SR) block cADPR-induced Ca2+ release in oocytes, and agonists of Ca2+ release from SR, such as Ca2+, caffeine, and ryanodine, also release Ca2+ from sea urchin egg homogenates (12, 14). High concentrations of caffeine or ryanodine, by depleting the same stores, desensitize the releasing activity of cADPR in intact eggs and in egg homogenates. Subthreshold concentrations of caffeine or ryanodine, which enhance the releasing effects of subsequent doses of these agonists, also enhance the releasing effect of subthreshold doses of cADPR and vice versa (3, 12,20).
These similarities between the properties of the cADPR- and ryanodine-sensitive Ca2+ release pathways indicate that sea urchin eggs have a common Ca2+-induced Ca2+ release route sensitive to caffeine, cADPR, and ryanodine. Accordingly, cADPR has been proposed to act as an endogenous agonist in sea urchin eggs (9, 14, 25), and it may act as an agonist of Ca2+ release in a variety of other cells as well (26). However, stimulation of Ca2+ release by cADPR in sea urchin eggs may involve at least two other proteins, besides the putative ryanodine receptors, that change their affinity toward cADPR in the presence of caffeine (29), suggesting that cADPR probably acts indirectly on the sea urchin egg putative RyR-channels. Furthermore, there is no information, to our knowledge, of the properties at the single channel level of the Ca2+release channels through which cADPR-activated release takes place in sea urchin eggs.
We have isolated a microsomal fraction from sea urchin eggs, and using planar lipid bilayers, we have investigated the presence of cADPR-sensitive Ca2+ channels in the isolated microsomes. Single channel experiments revealed channels in the microsomes that were activated by Ca2+, ATP, caffeine, and cADPR. The activation of the channels by cADPR was modulated by Ca2+, calmodulin, and Mg2+ and was blocked by ruthenium red.
Isolation of sea urchin egg microsomes.
Eggs were collected from seawater after electrostimulation of sea urchins (Tetrapigus nyger) into their coelomic cavity at room temperature. All subsequent steps were done at 4°C. After removal of jelly coats (10), eggs were washed once in Ca2+-free artificial seawater. This first wash was followed by two brief low-speed sedimentations in sucrose buffer (0.3 M sucrose, 0.1 M KCl, 20 mM 3-(N-morpholino)propanesulfonic acid, pH 7.0, plus protease inhibitors: 10 μg/ml leupeptin, 2 μg/ml pepstatin A, 5 mM benzamidine, 100 μg/ml soybean trypsin inhibitor). Eggs were homogenized in a Dounce homogenizer and sedimented at 2,000g for 20 min, and the resulting supernatant was sedimented at 12,000 gfor 20 min. The pellet was discarded, and the supernatant was sedimented at 100,000 g for 60 min. To remove the egg pigment echinochrome, the 100,000-g pellet was resuspended in sucrose buffer to a protein concentration of ∼10 mg/ml and fractionated in an agarose column (Bio-Gel A-0.5 m, Bio-Rad) equilibrated with sucrose buffer. The column exclusion fraction, containing the microsomes, was sedimented at 100,000g for 60 min, and the pellet was resuspended in sucrose buffer, quickly frozen in liquid nitrogen, and stored at −80°C.
Ca2+ release studies.
Ca2+ release was measured in microsomes actively loaded with Ca2+. For this purpose the isolated sea urchin egg microsomes were added at a protein concentration of 0.5 mg/ml to a fluorometer cuvette containing 1 ml of a solution of 1 mM Mg-ATP, 8 mM phosphocreatine, 4 U/ml of creatine kinase, 0.1 M KCl, and 20 mM 3-(N-morpholino)propanesulfonic acid-tris(hydroxymethyl)aminomethane (Tris), pH 7.2. To measure the concentration of free Ca2+([Ca2+]), 0.5 μM fluo 3 was added to this solution, and fluo 3 fluorescence was determined using a Shimadzu RF-540 spectrofluorometer with excitation and emission wavelengths of 506 and 526 nm, respectively. Because after vesicle addition, external [Ca2+] reached 5–10 μM, no extra Ca2+ was added to the incubation solution. After 30 min of incubation with Mg-ATP at 22°C, external [Ca2+] decreased to 0.3–0.4 μM. At this point, different agonists of Ca2+ release channels were added, and the changes in fluo 3 fluorescence were followed as a function of time. Calibration curves of fluo 3 fluorescence vs. [Ca2+] were done using solutions of known [Ca2+], which was determined with a Ca2+ electrode.
Bilayer experiments.
Planar phospholipid bilayers were formed from 5:3:2 palmitoyloleoyl phosphatidylethanolamine-phosphatidylserine-phosphatidylcholine. Fusion of vesicles to negatively charged Muller-Rudin membranes was performed as described previously (4), with slight modifications. Sea urchin egg microsomes were added to the ciscompartment solution, containing 5 ml of 200 mM CsCl, 0.1 mM CaCl2, and 25 mMN-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES)-Tris, pH 7.4. The other (trans) compartment contained 25 mM HEPES-Tris, pH 7.4. After channel fusion, evidenced by the emergence of current fluctuations a few minutes after the addition of the microsomes, the cis compartment was perfused with five times the compartment volume (a total volume of 25 ml) of a solution containing 25 mM HEPES-Tris, pH 7.4. After perfusion, 200 mM Cs-methanesulfonate, 0.5 mM Ca-HEPES, and sufficientN-hydroxyethylethylenediaminotriacetic acid or ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid to give the desired free [Ca2+], which was always checked with a Ca2+electrode, were added to the ciscompartment. The trans compartment was supplemented with 50 mM Cs-methanesulfonate after fusion. Voltage was applied to the cis compartment, and the trans compartment was held at virtual ground through an operational amplifier in a current-to-voltage configuration. Unless indicated otherwise, most single channel records illustrated here were obtained in asymmetric Cs-methanesulfonate solutions (200 mM cis/50 mMtrans) at room temperature (22–24°C), with membranes held at 0 mV. For analysis, data were filtered at 0.5 kHz using an eight-pole low-pass Bessel filter (model 902 LPF, Frequency Devices, Haverhill, MA) and subsequently digitized at 2 kHz with an acquisition system (Axotape, Axon Instruments, Foster City, CA).
Because the channels studied displayed four conductance levels, plus rapid and complex kinetics, the normalized mean current ( ) was used as an index of channel activity and was calculated as follows Equation 1whereI max is the channel maximal current amplitude andI mean is the mean current amplitude. Mean channel current and fractional times spent in each subconductance state (Pi ) were always calculated from steady-state records lasting ≥150 s. pClamp 6.0 (Axon Instruments) was used for the former calculations, and Transit software (Baylor College of Medicine, Houston, TX) was used for the latter. Furthermore, to establish that the four equal current levels observed corresponded to true subconductance states and not to four separate single channels, the following analysis was performed. For a binomial system made of four independent units, the frequency of occurrence of each conductance level is given by the following set of equations Equation 2 Equation 3 Equation 4 Equation 5 Equation 6In Eqs. 2-6 ,P o corresponds to the open probability of each unit andP c to the probability of the closed state. To calculateP o,Eq. 7 was used Equation 7From the calculatedP o the binomial frequencies for the open conductance states were estimated, and the resulting theoretical frequency values were compared with the experimental distribution of frequencies using a χ2 test.
IP3- and cADPR-induced Ca2+ release from sea urchin egg microsomes.
The sea urchin egg microsomes isolated according to the procedures described above actively accumulated Ca2+ at 22°C after addition of 1 mM Mg-ATP (not shown). The uptake of Ca2+ ceased after 20–30 min of incubation, when the [Ca2+] of the extravesicular solution reached 0.3–0.4 μM. At this point, addition of 5 μM IP3 to the actively loaded vesicles produced transient Ca2+ release, and subsequent addition of 1 μM cADPR induced a new transient Ca2+ release of smaller magnitude (Fig.1 A). The inverse sequence of agonist addition, first cADPR and then IP3, produced a transient Ca2+ release after addition of cADPR and a second release response to 5 μM IP3 smaller (Fig.1 B) than the release response obtained when 5 μM IP3 was added before cADPR (Fig. 1 A). These results indicate that the isolated microsomes have release pathways responsive to IP3 and cADPR. Addition of the Ca2+ ionophore A-23187 at the end of the experiment produced in all cases a marked increase in extravesicular [Ca2+] (Fig. 1). This response to the ionophore indicated that the vesicles had actively accumulated significant amounts of Ca2+ and that the increase in external [Ca2+] observed after addition of cADPR or IP3 corresponded in effect to Ca2+ release through pathways in the microsomal membranes.
Sea urchin egg microsomes displayed caffeine-sensitive Ca2+ channels.
Using planar lipid bilayers, we investigated the presence of Ca2+ channels in the isolated microsomes. For this purpose, we used experimental conditions similar to those routinely employed to detect ryanodine-sensitive Ca2+ channels in SR vesicles, such as pH 7.4 and high concentrations (up to 50 mM) of cations, Cs+ or Ca2+, in thetrans compartment (seeexperimental procedures). In the standard recording conditions (200 mM Cs+ cis/50 mM Cs+ trans) and in the presence of 12 μM Ca2+ in thecis solution, only single channels that exhibitedI max of 4–4.2 pA, with a low of 0.007, were observed (Fig. 2 A, top trace). Channel activity increased to a of 0.041 after addition of 2 mM caffeine to the cissolution (Fig. 2 A, bottom trace). Consistent channel activation by caffeine was observed in all single channels tested.
In contrast to the consistent activation of channel activity by caffeine, not all channels were consistently activated by ryanodine. After addition of 50–100 μM ryanodine over 12 μM Ca2+ in thecis solution, a distinct increase in channel P o was observed only in three of seven single channel experiments. Also, all our attempts to measure specific [3H]ryanodine binding to sea urchin egg microsomes were unsuccessful. For these reasons, we have not included the effects of ryanodine on single channel activity in this report.
Effect of cADPR on single Ca2+ channel activity.
Addition of cADPR to the cis solution containing 12 μM Ca2+ produced more stimulation of channel activity than 2 mM caffeine. Aftercis addition of 2 and 5 μM cADPR, channel activity increased from a basal of 0.006 to a of 0.039 and 0.125, respectively (Fig. 2 B). At this level of activity it became evident that cADPR-activated channels exhibited a complex behavior. Channel activity presented bursting kinetics, and fluctuations among a single closed state and four near-equal subconductance open states were clearly discernible with an amplified time scale (Fig. 2 B, bottom traces). Subconductance states, observed as well in native ryanodine-sensitive Ca2+channels from SR (1), were consistently observed in all channels studied. Several channel experiments (n = 4) revealed that, in the presence of 12 μM Ca2+ in thecis solution, increased as a function of cADPR concentration, reaching 0.095 ± 0.011 at 5 μM cADPR, the highest concentration tested. Significant channel activation over the control condition became noticeable at ≥0.5 μM cADPR (Fig.3 A, filled symbols).
To analyze in more detail the effects of cADPR on the distribution of subconductance open states, an experiment was performed using a higher Cs-methanesulfonate gradient (300 mMcis/50 mMtrans) to increase channel current and thus increase the signal-to-noise ratio of the experimental records. An increase in by cADPR as low as 0.2 μM was observed when 300 mM Cs+was used in the cis solution (Fig.3 A, open circles). This observation suggests that increasing Cs+ in the cis solution makes the channels more sensitive to stimulation by cADPR and is consistent with previous reports showing that the effect of agonists on mammalian ryanodine-sensitive Ca2+ channels is modulated by ionic strength (8, 31). In these conditions, a comparison of the fractional time spent in each subconductance state (Pi ) for the control and in the presence of two different concentrations of cADPR, 0.2 or 0.5 μM, revealed that cADPR increased channel activity by increasing all individualPi values (Fig.3 B). However, as reflected in the percent distribution of the states (Fig. 3 B, inset), the highest conductance state seemed to be somewhat more prevalent in the presence of cADPR. Further analysis of these experimental records in terms of the frequencies for the open conductance states (see experimental procedures) showed that they did not follow the pattern expected for a binomial distribution. In fact, as shown in Table 1, a comparison of the experimentally determined open substate frequencies (data of Fig.3 B) with those predicted byEqs. 2-6 revealed that in all these cases the χ2 test detected a significant difference between both frequencies. These findings indicate that the four current levels recorded in cADPR-activated channels are not due to a complex formed by four independent single channels.
Channels recorded in asymmetric Ca2+ gradients (12 μM Ca2+ cis/43 mM Ca2+ trans) were likewise activated bycis addition of 1 μM cADPR (Fig.4 A). Because 43 mM Ca2+, the concentration present in the transcompartment in these experiments, effectively blocks SR ryanodine-sensitive Ca2+ channels at the cytoplasmic surface (8, 31), the observed channel activity most likely corresponds to channels fused with their cytosolic side oriented toward the cis compartment.
A plot of maximal current values vs. voltage, obtained in channels activated by cis addition of micromolar cADPR in 12 μM Ca2+, revealed oocyte channel conductances of 47.5 ± 1.1 pS in asymmetric Ca2+ solutions and 155.0 ± 8.0 pS in asymmetric Cs+ solutions (Fig. 4 B). The displacement of theI max-voltage curves toward the right after transaddition of Ca2+ to channels recorded in Cs+ (data not shown) implies that the channels were more selective for Ca2+ than for Cs+. The higher selectivity for Ca2+ than for monovalent cations such as Cs+, K+, or Na+ is a well-known feature of mammalian ryanodine-sensitive Ca2+channels (8). For practical reasons, because higher currents were obtained in Cs+, making readily visible the different subconductance states and increasing the differences between the lowest subconductance state and baseline noise (as shown in Fig. 4 A), all subsequent channel experiments were done in asymmetric Cs+ solutions (200 mMcis/50 mMtrans).
Lack of effect of IP3 on channel activity.
Addition of 2 μM IP3 to thecis solution containing 12 μM Ca2+ had no effect on channel activity and did not interfere with subsequent channel activation by 5 μM cADPR (Fig. 5 A, middle traces). In contrast, decreasing [Ca2+] in thecis solution from 12 to 0.72 μM in the continuous presence of 2 μM IP3 and 5 μM cADPR produced a significant reduction in channel activity, decreasing from 0.115 to 0.005 (Fig. 5 A, bottom trace). This decrease in channel activity, which took place when [Ca2+] in the cis solution was lowered to levels that optimize the Ca2+ release response to IP3 in sea urchin egg homogenates (5), indicates that these cADPR-activated channels are not responsive to IP3. In addition, this reduction implies that stimulation by cADPR requires >0.72 μM Ca2+ in thecis solution, as reported in further detail below.
Effects of calmodulin, ruthenium red, and heparin on channel activity.
In a separate experiment, addition of 4 μg/ml (0.24 μM) calmodulin to a channel already activated by 2 μM cADPR in 12 μMcisCa2+ did not modify channel activity (Fig. 5 B, 3rd trace). Yet, further addition of ruthenium red produced a marked inhibition, lowering from 0.063 to 0.005 (Fig. 5 B, bottom trace). Likewise, no effect of cis addition of calmodulin on the activity of cADPR-activated channels was observed when the [Ca2+] of thecis compartment was lowered to 0.72 μM (records not shown). Because cADPR-activated Ca2+ release in sea urchin egg microsomes has an absolute requirement for calmodulin (22, 23, 27), endogenous calmodulin may be associated with the channels fused in the bilayers. That this is most likely the case is shown by the experiments described below.
Addition of heparin to the cissolution containing 12 μM Ca2+plus 5 μM cADPR had a marginal stimulatory effect on channel activity and did not interfere with subsequent channel inhibition by ruthenium red. Thus channels (n = 2) that, after addition of 5 μM cADPR to the cissolution containing 12 μM Ca2+, increased their activity from a of 0.010 ± 0.001 to 0.083 ± 0.026, on addition of 300 μg/ml heparin increased their only to 0.125 ± 0.019. This small increase in channel activity brought about by 300 μg/ml heparin was not statistically significant. Further increasing heparin to 600 μg/ml had a negligible effect on channel activity, as reflected by a of 0.127 ± 0.028. Subsequent addition of 30 μM ruthenium red decreased channel activity to a of 0.008 ± 0.004.
Ca2+dependence of channel activity.
In the absence of cADPR, varying cis[Ca2+] from 0.7 to 270 μM had a modest effect on single channel activity (Fig.6, open circles); increased from <0.003 in 0.7 μM Ca2+ to ∼0.01 in 12 μM Ca2+. Channel remained constant at 12–30 μM cisCa2+. Further increasing [Ca2+] to 270 μM produced some decrease in , to an average of 0.006.
In contrast, in the presence of 1 μM cADPR, increasingcis[Ca2+] to 12 μM had a marked stimulatory effect on channel activity (Fig. 6, filled circles), as reflected by an increase in to ∼0.035. As observed in the absence of cADPR, channel activity remained constant at 12–30 μM cisCa2+. Further increasingcis[Ca2+] to 60 μM reduced to ∼0.020.
In the experimental conditions used to record channel activity, 1 μM cADPR did not activate the channels at ≤0.7 μM Ca2+ (Fig. 6). Yet, as shown in Fig. 1, 1 μM cADPR was effective in eliciting Ca2+ release from sea urchin egg microsomes actively loaded with Ca2+ and bathed in solutions containing ∼0.4 μM Ca2+. Furthermore, concentrations of cADPR as low as 10 nM have been reported to activate Ca2+ release from sea urchin egg homogenates actively loaded with Ca2+ (13). Because vesicular Ca2+ release and bilayer experiments were done in different conditions, such as the presence of millimolar Mg2+ and ATP in the external solution when vesicular release was measured, we investigated whether cADPR stimulation of channel activity was modified by addition of ATP or Mg2+ to thecis solution.
Stimulation of channel activity by ATP.
Addition of 2 mM ATP to the cissolution containing 12 μM Ca2+produced a significant stimulation of , from 0.007 to 0.022 (Fig. 7). Subsequent addition of 0.5 μM cADPR did not stimulate further channel activity but produced a small reduction in channel to 0.015 (not shown). Even increasing cADPR to 2 μM in the presence of ATP did not stimulate channel activity, because remained at 0.015 (Fig. 7, top, 3rd trace). After extensive washing of the ciscompartment (see experimental procedures) and addition of 12 μM Ca2+ to thecis solution, the channel regained its low activity, with a of 0.005. At this point, addition of 1 μM cADPR increased to 0.032 (Fig. 7,bottom). These results indicate that the stimulatory effect of ATP on channel activity did not add to that of cADPR and was wholly reversible. In concordance with these results, several experiments revealed that cisaddition of 1–2 mM ATP to cADPR-activated channels did not stimulate further channel activity (data not shown).
Stimulation of channel activity by cADPR plus Mg2+.
To analyze the effects of Mg2+ on cADPR-activated Ca2+ channels,cis[Ca2+] was held at 24 μM, a concentration that was within the range of [Ca2+] that produced optimal channel activation. To avoid changes in [Ca2+] after Mg2+ addition, ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid was used as a buffer. Addition of 1 μM cADPR to a single Ca2+ channel increased from 0.003 to 0.020 (Fig.8 A). Subsequent addition of 1 mM Mg2+to the cis solution markedly enhanced channel activity, increasing 12.8-fold, from 0.020 to 0.256 (Fig. 8 A). Addition of Mg2+ in the absence of cADPR had no effect on channel activity (not shown).
Yet, not all channels studied displayed the same marked activation by Mg2+. In the example of the single channel illustrated in Fig. 8 B, after addition of 1 μM cADPR in 24 μMcisCa2+, the channel increased its activity from a of 0.009 to 0.012 (Fig. 8 B,top and middle traces). Subsequent addition of 1 mM Mg2+ produced only a 4.4-fold stimulation of channel activity, to a of 0.053 (Fig.8 B, bottom trace).
The difference between these two types of responses is further illustrated in Fig. 9. All channels that presented a more marked activation by Mg2+ (Fig. 9, filled squares) were clearly activated by a cADPR concentration as low as 0.1 μM, with an apparent saturation at 1–2 μM cADPR. In contrast, the channels less activated by Mg2+ required >0.5 μM cADPR for discernible activation and did not seem to saturate at a cADPR concentration as high as 2 μM (Fig. 9, open circles). These results indicate that, in the combined presence of 24 μM cisCa2+ and 1 mM Mg2+, some of the channels markedly increased their sensitivity to activation by cADPR. This finding suggests that one of the additional components present in Ca2+ release experiments makes the channels more responsive to cADPR. However, the differences in the requirements for higher cis[Ca2+] to observe channel activation by cADPR in bilayers, as opposed to release experiments, cannot be attributed to Mg2+. Whencis[Ca2+] was decreased to the submicromolar range, channels were not consistently activated by addition of cADPR, even in the presence of 1 mM Mg2+ (data not shown).
A study of the response of the channels less sensitive to activation by Mg2+ showed that concentrations of Mg2+ >1 mM were less effective in enhancing channel activation by cADPR, and channel activity in 9 mM Mg2+ was lower than in its absence (Fig. 9, inset).I max was reduced as well by >7 mM Mg2+ (data not shown).
Effects of calmodulin antagonists plus Mg2+ on channel activity.
Channels activated by cADPR were not affected by calmodulin addition (Fig. 5), even at a concentration (4 μg/ml) that induces near full activation of Ca2+ release in sea urchin egg microsomes (22, 23, 27). Because calmodulin seems to be essential for activation of Ca2+release by cADPR in these microsomes (23), the lack of effect of calmodulin on single channel activity may reflect the presence of endogenous calmodulin tightly associated with the channel protein. To test this hypothesis, we examined the effects of the calmodulin antagonist W-7 (17) on channel activity. A single channel that displayed a of 0.274 in the presence of 24 μM Ca2+, 1 mM Mg2+, and 2 μM cADPR in thecis solution (Fig.10, top trace) decreased its activity to a of 0.047 aftercis addition of 10 μM W-7 (Fig. 10,middle trace). To test for the specificity of this effect, 10 μM calmodulin was subsequently added to the cis solution. After addition of this large excess of calmodulin, the channel increased its to 0.223, which is similar to that measured before W-7 addition (Fig. 10,bottom trace). Inhibitory effects comparable to those obtained with W-7 were observed with use of compound R-24571 (calmidazolium), a different calmodulin antagonist (data not shown). These results support the above hypothesis that channels fused in the bilayer contain sufficient endogenous calmodulin to support channel activity.
The results described here demonstrate that microsomes isolated fromT. nyger eggs contain Ca2+ channels that were activated by cis addition of caffeine, micromolar Ca2+, or millimolar ATP. Channels were also activated bycis addition of micromolar cADPR, and stimulation by cADPR was modulated bycis[Ca2+] and by Mg2+.
Comparison with mammalian RyR-channels.
Activation by caffeine, ATP, and cis[Ca2+] in the micromolar range (8, 11, 12) is characteristic of vertebrate RyR-channels. Furthermore, as described for RyR-channels isolated from mammalian skeletal muscle (11), the egg channels displayed a bell-shaped Ca2+ dependence of in the presence of cADPR. In addition, the egg channels were inhibited by ruthenium red and displayed multiple subconductance states, behaviors displayed as well by the mammalian RyR-channels (1, 8). On the basis of these similarities, it is tempting to assign the cADPR-activated channels from sea urchin eggs to the ryanodine receptor family. Although the conductance of the cADPR-activated egg channels (50 pS) was lower than the conductances described for RyR-channels from mammalian skeletal and cardiac muscle (100–120 pS) (8), this difference may not be very significant, because variations in the conductance of the mammalian channels have been reported (2). Yet, in contrast to the well-reported effects of ryanodine on RyR-channels (8, 31), we did not observe consistent modulation of the sea urchin channels by ryanodine. We have no explanation for this lack of consistent effects of ryanodine on channel activity.
Channels activated by cADPR were not activated by IP3.
The egg channels activated by cADPR were not IP3-gated channels, since addition of IP3 (2 μM) to thecis chamber had no effect on channel activity. However, IP3 addition to sea urchin microsomes actively loaded with Ca2+ caused significant Ca2+ release, indicating that the microsomes described in this study have release pathways responsive to IP3. Accordingly, a straightforward interpretation of the lack of effect of IP3 on channel activity is that the IP3-gated channels of sea urchin eggs (not described so far in bilayers) are different from the Ca2+ channels activated by cADPR studied in this work. It is pertinent to mention in this regard that there is significant evidence indicating that the cADPR- and IP3-sensitive Ca2+ release pathways of sea urchin eggs are different. 1) Ruthenium red and procaine, two antagonists of Ca2+-induced Ca2+ release in SR, inhibit cADPR-sensitive Ca2+ release in egg homogenates without affecting IP3-sensitive release (12, 13).2) Even high concentrations of IP3 do not alter the Ca2+-releasing activity induced by cADPR (12). 3) cADPR-induced Ca2+ release is insensitive to heparin (13, 24), a competitive inhibitor of IP3 binding to its receptor. Furthermore, the lack of effect of heparin on Ca2+ release is in agreement with the negligible effect of heparin on channel activity found in this work, even at heparin concentrations that completely inhibit IP3-sensitive Ca2+ release.
Ca2+dependence of channel activity in the presence of cADPR.
Sea urchin egg Ca2+ channels were moderately activated by increasing cis[Ca2+] from 0.7 to 30 μM, but in the presence of micromolar concentrations of cADPR, channel activation by increasing cis[Ca2+] in this range became more prominent. These results agree with a recent report describing significant enhancement of cADPR-gated Ca2+ release rates in sea urchin eggs by increasing [Ca2+] (16).
In the standard experimental conditions used to record channel activity, 1 μM cADPR did not activate the channels at ≤0.7 μM Ca2+. In contrast, we found that 1 μM cADPR was effective in eliciting Ca2+ release from microsomes actively loaded with Ca2+ and bathed in solutions containing 0.3–0.4 μM Ca2+. These results are in agreement with a previous report showing that cADPR induces Ca2+ release from sea urchin egg homogenates at submicromolar [Ca2+] (5). Nonetheless, it is important to note in this regard that Ca2+ release from microsomal vesicles actively loaded with Ca2+is measured under experimental conditions quite different from those prevailing in bilayer experiments. In release experiments,1) the vesicles have actively accumulated significant luminal Ca2+,2) they are bathed in solutions containing millimolar Mg-ATP, and 3) other vesicular proteins are present that may or may not fuse with the channels into the bilayers. Each one of these factors might in principle enhance the effect of cADPR at submicromolar [Ca2+], since all of them regulate the activity of mammalian RyR-channels (8, 18, 31). However, even in the presence of 1 mM Mg2+ channels were not consistently activated by cADPR in submicromolar [Ca2+], making unlikely Mg2+ by itself as the cause of the different Ca2+sensitivity. Whether the other factors mentioned above are responsible for the differences between bilayer and vesicular release experiments should be investigated. In particular, the effects of luminal Ca2+ should be studied, since we have preliminary results (not shown) indicating that cADPR was more effective in stimulating channel activity when thetrans compartment contained 50 μM Ca2+ in addition to 50 mM Cs+. Additionally, other factors that enhance the effects of cADPR on Ca2+ release from sea urchin egg homogenates, such as the oxidation state of critical SH groups (13, 28) or the presence of palmitoyl-CoA (6), may enhance the channel response to cADPR at low [Ca2+].
Effects of Mg2+ on channel activity.
It has been reported that 1 mM MgCl2 is required for optimal activation of Ca2+ release by cADPR (12-15) and that cADPR-activated Ca2+ release from sea urchin egg homogenates is inhibited by millimolar concentrations of Mg2+ (6, 15). In agreement with these reports, our results indicate that Mg2+ presented a dual effect over the activity of cADPR-stimulated channels. Thus 1 mM Mg2+ increased markedly the stimulatory effect of cADPR on channel activity, but at higher concentrations it was less effective and eventually became inhibitory. Furthermore, in the presence of 1 mM Mg2+, the sea urchin egg channels displayed two types of responses toward activation by cADPR. Why channels presented these two different responses is not clear and should be the subject of future investigation.
The results of this work indicate that sea urchin egg microsomes possess Ca2+ channels that share similar properties with vertebrate RyR channels, such as activation by Ca2+, ATP, and caffeine and inhibition by ruthenium red. Because activation of oocyte channels by cADPR was enhanced by Ca2+, we propose that these Ca2+ channels of sea urchin eggs constitute an intracellular Ca2+-induced Ca2+ release pathway in these cells.
We thank Dr. M. T. Nuñez for helpful criticism of the manuscript.
Address for reprint requests: C. Hidalgo, Centro de Estudios Cientı́ficos de Santiago, Casilla 16443, Santiago 9, Chile.
This study was supported by Fondo Nacional de Ciencia y Tecnologı́a Grants 2950037, 1940369, and 1970914, by European Community Grant CI1CT940129, and by institutional support to the Centro de Estudios Cientı́ficos de Santiago from a group of Chilean companies.
- Copyright © 1998 the American Physiological Society


text:
gbvintage's Profile
Shopping on Etsy is great way to find specific and unique items !!!
I'm artist. My art background has led me into several different styles of expression. I love to create and collect rare stuff. I have grown up in Soviet times. I still have many things from that period. Signs of the time have made them unique - with their own stories. Now, for your pleasure, I have lot of boxes with interesting and rare stuff - it's time some of gb vintage share with you.Thank you for enjoying them with me. My other shops on etsy:
Since February you are welcome to warmth your body and soul. Hand knitted socks made by my mother - enjoy!!! ~~~Handmade socks~~~
- Female
- Born on April 27
- Joined December 30, 2010
Favorite materials
plants, stones, essential oils, Eco products, woods, amber, buttons, laces, threads, shells, wool, vintage materials


text: Meta Battle Subway PokeBase - Pokemon Q&A Login Register All Activity Questions Unanswered Tags Users Ask a Question Rules Chat What is my pokemon's hidden power's base power? 0 votes 525 views Ev's: Speed: somewhere around 13-16 Sp. Def: 3 HP: 4 SpA: 1 Elekid (♂) Level 11 Somewhat stubborn. Timid Nature Stats: HP: 31/31 Atk. 18 Def. 16 Sp. Atk. 22 Sp. Def. 20 Speed 29 HP type is Psychic Lotad's Ev's: Haven't trained it yet Often dozes off. Modest Nature Lotad (♂) Level 5 Atk. 8 Def. 8 SpA. 11 SpD. 10 Speed 9 HP type is Rock. If you can help, please give suggestions for both pokemon! If I need IV's, please tell me and I will do so! Thanx! hidden-power asked Sep 9, 2011 by [MEGA]StellarLucario edited Sep 10, 2011 by [MEGA]StellarLucario IVs please commented Sep 9, 2011 by Tysonyoshi I don't really have exact IV's, but I have a range. What should I do to find exact IV's? commented Sep 10, 2011 by [MEGA]StellarLucario I saw SF's Old question on his Cofagrigus' HP, i'm taking some of his ideas. commented Sep 10, 2011 by [MEGA]StellarLucario To tell they need to be about lvl 50 or Lvl 100 commented Sep 10, 2011 by Speed freak thanx SF commented Sep 12, 2011 by [MEGA]StellarLucario Please log in or register to add a comment. Please log in or register to answer this question. 1 Answer 2 votes Best answer Sorry but it's impossible to tell. You need to know the exact IVs and EVs of the Pokemon. The best thing is to save your game then teach HP and try it out to see how much damage it does. There is also a guy who will tell you the type. answered Sep 10, 2011 by Pokemaster selected Dec 16, 2012 by Mewderator (****censored****) THIS ALWAYS HAPPENS!!!!! commented Sep 10, 2011 by [MEGA]StellarLucario Please log in or register to add a comment. Related questions What is Hidden Power's highest Base Power? Is Hidden Powers base power always 70? Where in pokemon platinum can I check my pokemons hidden power's type? How to judge hidden powers power? Where in Pokemon Platinum can I check hidden powers power? How do I know what's my Hidden Power base power in Black & White? I want to figure out my pokemon's hidden power, but I don't know my IV's or EV's How do you know what is the base power of your hidden power? Useful Meta Posts How to change your username How to add a user avatar Why was my post hidden? The points system Question etiquette Flags vs Downvotes Anime FAQ


text:
Watch shots of Milo Ventimiglia as a bright med school student trapped in a deadly game at the titular program.
The time has come for MGM to reveal more glimpses of its upcoming crime thriller "Pathology." The studio has finally brought up a new trailer for the movie on the net via video sharing website YouTube.
Running over two minutes, the trailer, as you can see below, follows Milo Ventimiglia's character Ted Gray as he joins one of the nation's most prestigious Pathology programs. Several characters are also introduced while viewers get provided with the basic story of the flick.
Directed Marc Schoelermann, "Pathology" centers on a group of medical students who devise a deadly game to see which one of them can commit the perfect murder. Pic will open in theaters on November 30 this year alongside "Cassandra's Dream" and "Perfect Christmas", among others.


text:
I wasn't keeping track until June 1st. From 6/1 - 12/31 it was 1585 miles.
2013 Mileage....where did you end up?
1466 for my first year of running. Here's to more next year!
2890km or 1791 miles, 1st full year of running... hopefully 2014 will be even more...
2470.5 for my first full calendar year of running, I'm pretty happy with that.
3,188 for me. Mileage PR for me, also helped me to PR in the marathon, 10k and 5k.
2305. highest 274 mpm / 72 mpw.
2,472 km for the year. I lost a lot of ground between mid-May and August, although I never had a yearly goal to begin with. I think I'll aim for 3,000 this year - it'll keep me training through the summer heat.
3925 miles for me.
8 marathons and a new pr, 1st 50 miler and 1st 100 miler. Pr'd my half this year too. This was my 5th year of running and 4th year of marathoning. I also did my first set of doubles, ran a marathon in New Hampshire on Saturday, drove to Maine and ran a marathon in Maine on Sunday. Well under 4 hours for both of them too.
Best month July 441 miles.
Worst month November 247 miles
2215 for the year
112 for December
peak was 303 for July.
The wheels fell of the wagon shortly thereafter.
81 days left until spring. Can't wait until pitchers and catcher report to spring training. In the meantime it is a pipeline of nor'easters and lots of treadmill running.
2,628.6 miles, 50.55 per week average. Up from 2005 miles in 2012. Highest month was 309 in May, followed by my marathon PR on June 22nd.
2556 miles, my highest ever. More than 300 miles higher than last year. This was a little over 400 hours of running (400:12:59). Wrapped up December with 267, my 2nd highest ever. Highest was September with 283. Ran every day in December due to the Holiday Running Streak.
It was my best running year so far, with PRs at every distance and a BQ-11. Also a bunch of AG awards including my first ever AG award in a marathon.
I suspect these things are related. Congratulations on the excellent year!
2977 miles (4792 km) for 2013.
Not a bad year.
1,535 total miles
8 races:
Marathon (5th in age)
HM (3rd in age)
30K trail (2nd in age)
15K (1st Masters)
Two 10Ks (one 1st and one 2nd in age - including a Fat Old Man PR)
5K (1st in age)
Plus my 6th consecutive trip up Grandfather mountain for the Bear.
Month: 243 / Year: 3010
Five year average over 3000 miles.
Month: 243 / Year: 3010
Five year average over 3000 miles.
and that was with a little break if i remember correctly.
1925 and looking like a little leaguer compared to most here! Still knocked out 3 PRs at 10k, half, and full. Took my full PR from 4:39->3:44. So a good year for me.
Pretty good year: 2413.
Lousy December: 86 (flu, pneumonia, gimpy hip, ski vacation...)
- rovatti
Month: 243 / Year: 3010
Five year average over 3000 miles.
and that was with a little break if i remember correctly.
You do.
3017 mi
PR'ed marathon twice (Ogden, then St George @ 3:33) and PR'ed the only 10k that I ran (42:13)
My monthly mileage stayed between 245-280 each month, with the lowest during marathon race months due to taper and recovery.
Oh, and if it counts for anything I hit the slopes 22 times in 2013 snowboarding as well :-)
1746 not bad first year back shooting for 2000 in 2014


text:
We help you find a Wadhurst conducting teacher. Conducting lessons are a brilliant method to begin a stimulating new hobby, acquire a new skill and attain confidence in conducting. Try our site to find Wadhurst conducting tutors and select which matches your requirements so you can start your conducting tuition in Wadhurst straight away!
This is the best way to contact Wadhurst conducting teachers in the UK, helping you find Wadhurst conducting lessons close to home and Wadhurst tutors to assist with any instrument like piano, violin or guitar. We can help you find Wadhurst conducting tuition or a Wadhurst conducting teacher to prepare for conducting exams and develop your conducting skills! We order Wadhurst conducting teachers local to you according to their tuition charges and distance from you.
If you are a conducting teacher wishing to offer your conducting lessons in Wadhurst or throughout the UK, please register with First Tutors: Music or learn about us here. Becoming a Wadhurst conducting teacher is a great way to re-affirm your own skills through providing conducting tuition while helping others, all in the Wadhurst area!


text:
I do and I feel guilty lol. How bout you?
Most Helpful Girl
... are Jewish folk not supposed to eat cheeseburgers?00|00|0
Not allowed to mix beef with milk
Huh. Didn't know that.
It's valid for Orthodox Jews. Many other Jews don't give a damn about it. But there are 'solutions':
Most Helpful Guy
I'm not Jewish but I went to a 90% jewish student school. I do not remember ever having cheeseburger on the menu. Do you guys not eat beef or cheese?00|00|0
We can but not together
- Show AllShow Less
It's about not mixing an animal with it's mom's milk lol
That makes sense actually
It made me realize how sick we are


text:
Emerson AOR2 Combat Shirt & Pants Set
The item described in this review is an airsoft replica of Crye's AC version of their combat shirt and pants. It is quite possible that several improvements have been done to their product line to arrive at the product that I am looking at now. Since I have no basis or history available to me due to the nature of reproduction items, I will go with what knowledge I have and will update with further reviews as necessary.
From Crye Precision's website:
"The design that started a movement. When we developed the first version of our signature shirt, we had no idea that it would lead to such widespread adoption of our basic design. The Combat Shirt is a purely operational fighting upper garment. This shirt is designed specifically to be worn under body armor. It keeps you cool with a lightweight high-performance flame resistant DRIFIRE® torso, and reinforced Mil-Spec NYCO ripstop sleeves designed for use with our patented removable Combat Elbow Pads (sold separately). Features a zip collar that allows easy donning and doffing while keeping slings, straps, and brass off your neck.
"Designed as a purely operational fighting lower garment, with none of the compromises of garrison wear, they are aggressively cut for maximum mobility. These combat-proven pants feature a padded waist band and high-mobility stretch panels in the knee and lower back. 10 pockets. Designed for use with our patented removable Combat Knee Pads (sold separately). Base fabric is Mil-Spec 50/50 NYCO ripstop. Stretch fabric is durable a 4-way stretch woven. Zipper fly with Velcro® cinch tab"
This replica set attempts to have exactly the same features as the product being copied, however falls slightly short of that mark. I have obtained the set from Blackhog Gears.
Integrated kneepads
These are great and very comfortable. They have thickened the pads significantly in later releases, which helps with joint protection.
Breathable stretch panels around the knee area:
This is where Emerson falls short of the mark. They've included a lightweight, breathable material for the panels, however this material does not stretch and therefore affects the comfort of the item in question. This is related to the tightness and mobility issues described in the section about how the item fits.
Teflon coating
The camouflage ripstop fabric used in this item is coated with Teflon for additional stain resistance. The downside is that the Teflon will come off after a few washes. On the bright side, it should make the fabric a bit softer and more breathable.
This part surprised me. The uniform fit me correctly on the sleeves and pant length. The waist was ok, but it is still quite tight around the thighs, which makes it a bit hard to move. I will have to break these in if possible.
As with the usual Emerson combat uniforms, these look quite sturdy and should be able to put up with the abuse we put our uniforms through. I have a multicam and AOR1 set from Emerson and they are holding out quite well.
The colors are fairly close to the real thing. Slightly darker than the real deal but should lighten with some use.
(Emerson replica compared to the an authentic NWU type III uniform)
Vastly improved are the kneepads. The inner foam parts are thicker and provide better padding. The velcro seems to be stuck on with better adhesive and did not come off when I pulled it from the socket. (edit: I take it back! the glue, while improved, was still easy to remove. I glued the velcro back on using mighty bond. They also use the same thin strips, bleh!)
(Emerson replica compared to the an authentic NWU type III uniform)
Similar material to previous uniforms that I have from them. They used a tough nylon/cotton ripstop material. It isn't authentic NWU Type III cloth but close enough. The material that is supposed to go into the stretch panels isn't quite stretchy at all, and this is why it is tight around my thighs and difficult to lift my legs with.
This is really a nit-pick but the collar doesn't fit quite as correctly as their previous uniforms.
At PHP 3,700.00 (US$90.00) this replica uniform is not cheap, but it is the cheaper alternative to buying the real thing. (expect to spend upwards of $1000 for a set on eBay so unless you have a Navy Seal friend or are ridiculously rich, forget it) Other reproductions don't quite hold a candle to this except for ones you can get from Kevin Toysoldier, who makes particularly excellent reproductions. Expensive, but worth it.
Bottom line: It's not the best but get it if you want AOR2 and don't want to spend an exorbitant amount of money.
In these pictures: the uniform is compared to an authentic NWU Type III field shirt and authentic NWU Type III trousers made by Ur-Tactical.com.
- Login to post comments
- Printer-friendly version
What about the Allwin
What about the Allwin replica? They are very well made for their price. I'd rate them between Emerson and Toy Soldier.
- Login to post comments


text:
The NECR GP40-2CU 3015 idles with a Green Mountain Railroad passenger consist in tow at Montpelier, Vermont late on May 4. The train was put together for a special legislative luncheon for Railro... (more)
Running long-hood forward, NECR ``chip train`` with freshly painted NECR# 3015, brings empty woodchip hoppers north to Swanton as it rolls high above the Lamoille River/ Arrowhead Mountain Lake o... (more)
Fresh out of the paint booth, New England Central GP40-2CU (GWI's designation of the unit - GP40-2W for the purists) 3015 heads south past North Junction at St. Albans, Vermont on the way to Burli... (more)
Fresh out of the paint booth, New England Central GP40-2CU (GWI's designation of the unit - GP40-2W for the purists) 3015 rolls through the rural Fonda, Vermont, north of St. Albans on the Swanton... (more)
Fresh out of the paint booth, New England Central GP40-2CU (GWI's designation of the unit - GP40-2W for the purists) 3015 is seen high above the Lamoille River on the Georgia High Bridge with Frid... (more)
With the Burlington Electric plant barely visible through the tree growth, the NECR 500 chip train is seen nearing completion on the unloading of the high capacity rapid discharge wood chip cars i... (more)
Engineers Construction Inc. (ECI) crews are busy at work replacing the Elm Street multi-track crossing in St. Albans on the New England Central Swanton Subdivision. As crews positioned a section ... (more)
New England Centrals new parents colors lead NECR 323/324 trains for the first time tonight as 3809 leads 323 through Randolph at 02:18. Repainted in the roads St. Albans, VT paint booth, G&Ws col... (more)
Three separate sets of motive power are seen by the sanding tower in St. Albans, Vermont, on Sunday, April 22, including the dead line with the NECR SW1500 2340, TP&W GP40-2W 4055 and the hulk of ... (more)
The first unit to wear the Genesee & Wyoming corporate colors for the Cape Breton & Central Nova Scotia is the former GEXR GP40 4022 turned CBNS 3022. The unit is seen at St. Albans, Vermont bein... (more)
Former Indiana & Ohio logos have been removed from the big SD40T-2 that has become the property of the New England Central Railroad. Sporting the NECR logos on the long hood but awaiting the cust... (more)
Sporting new G&W paint, New England Central GP40-CU 3809 approaches Norwich Tunnel in Norwich, CT on the NECR Palmer Sub. This is the first G&W painted unit to operate on the NECR south of Palmer... (more)
A rare matched set of New England Central Railroad GP38s are currently assigned to White River Junction, Vermont for the 600 and 601 trains based there. NECR 3845 and 3850 are working a cut of ca... (more)


text:
Syria says deployment on Lebanon border to fight smuggling
Follow >
Click here to add Abdullah bin Abdulaziz as an alert
Disable alert for Abdullah bin Abdulaziz,
Click here to add Jean Qahwaji as an alert
Disable alert for Jean Qahwaji
Lebanon's Army Commander Gen. Jean Qahwaji said Syria had informed Lebanon in advance of deploying troops off the northern borders with the aim on fighting smuggling. According to Naharnet, Qahwaji relayed the information to Premier Fouad Saniora who returned home Tuesday from a visit to Saudi Arabia during which he met King Abdullah Bin Abdulaziz.
Qahwaji reportedly said the Syrians had informed him that the move has "no other aim but upgrading procedures to combat smuggling."
Earlier, a Lebanese military spokesman said the Syrian authorities have assured the Lebanese army that the build-up is aimed at cracking down on smuggling and other crime along the border.


text:
very nice..
After successfully creating black chocolate cake for 18th birthday wishes with name picture with our online image editor app. Now can share it to your social media networks like Facebook, Twitter, Google, Pinterest, etc. and set as profiles dp. You can also download this image directly to your Smartphone, Pc etc. This will surely like to your friends and family member.
very nice..
I want my dick suck bitches
Happy birthday
Happy wala Birthday Deva
Happy Birh Day
Happy birthday angels Karuvachchi
Happy birthday my dear Divya …..
very nice


text:
Just a day back, South Korean giant LG announced that its forthcoming G3 handset will probably be launched in the second quarter of 2014. Well the good news is that LG has already started sending out the official invites for the launch event on May 27 in San Francisco, New York and London simultaneously. Also Singapore, Seoul and Istanbul will follow next, claims a report from The Verge. The South Korean company has scheduled events in six countries across the world on May 27 and May 28.
LG G3- Press Invite
The invite says it all as it reveals that the announcement will largely focus on the LG G3. LG already stated that the G3 will be introduced in the second quarter of this year. The press invitation seems to have been inspired from the forthcoming handset. The dark metal finish color in the background on the invite, allegedly reveals that the company using a new premium material.
Recommended: Google Could Replace Nexus Devices with Android Silver Range, Says Reports
Generally, LG-made phones come encased in plastic chassis, which isn't a bad thing but now consumers are eagerly waiting for big change. Furthermore, the teaser image reads," To be simple is to be Great" that should be linked with the handset's updated UI, design and especially build quality.
LG G3- What to expect?
LG G3 has been detailed in many reports since the beginning of this year. The rumored LG G3 will be a premium device with a 2,560 x 1,440 display. The exact screen size is unknown, but if gossip mongers are to be believed the G3 may have a massive 5.5-inch display. The LG G3 will offer sharper display and beefier processor.
The G3 would also be powered by a quad-core Qualcomm Snapdragon 801 processor coupled with 3GB of RAM and at least 16GB of on-board storage. Moreover, the phone could adopt a brushed aluminum chassis, instead of a regular polycarbonate plastic body. The device is also said to come with a 13-megapixel rear-facing camera with OIS + feature.
Recommended: LG G3 Leaks Again: Release Date, Features and Specs [RUMOR ROUNDUP]
The biggest highlight of the G3 could be its updated 'flat' Optimus UI. The LG G3 is expected to be made available in Golden color, besides regular color options. Also, LG G3 may follow Samsung and Sony with the waterproof body.
LG needs to establish itself as a major smartphone player with a solid marketing plan, which the company currently lacks when being compared to Samsung or Apple. But it is the new flagship G3 that the company hopes will help it re-establish itself as a real challenger to market leaders Samsung and Apple. Stay tuned to GizBot for more updates!


text:
First Steps Checklist
Please forward the following materials to email@example.com immediately. We cannot guarantee marketing placements if materials are received after November 1, 2017.
Next steps
- Register your attendees: If using a discount code or bulk company pass arrangement, please register your team ASAP.
- Email your logo:
- High-resolution company logos (ALL sponsor tiers)
- Vector formats FOR PRINT: .EPS, .PDF, .AI
- Bitmap formats FOR DIGITAL: .JPEG, .PNG, .TIFF
- Promote your involvement: Web banners are available here (INSERT LINK) for placement on your website or social media channel.
- Plan your show announcements: Have a new product, service or company development to announce? Do it at NamesCon!
- Submit any relevant press releases to NamesCon producers so we can share with registered media in advance of the conference.
- Complete the Sponsor Spotlight Q&A for your company be featured on domaininvesting.com


text:
Our mornings begin with thirty minutes of outdoor exploratory play upon drop-off (parents welcome to stay, too!) From here we say goodbye to parents and travel to our forest camp, playing games along the way. Our opening circle introduces the day's theme, followed by snack and story time. Then it's off for the adventure of the day: hiking to a favorite lookout, searching for animal dens or tracks, building a fort, or exploring the latest signs of the season. We are open to wherever our senses and our imagination may take us!
See our Parent Handbook, below, for detailed information.
To get an insider-look at Forest Preschool, explore our blog: http://greenmountainaudubon.wordpress.com
Winter/Spring Session Dates: Fridays, January 20 through June 2, 2017
Fall 2017 session date
Time: 9 am to 1 pm, pack a lunch!
Cost: $450 for non-members, $380 for Audubon members (You may become a member here; family membership required.)
Ages: 3 to 5 years
Maximum enrollment 12 children
Registration Process:
1. Contact Debbie Archer to register.
2. Complete Health Form and Release Form (see "Downloadable Resources" below) and submit to Audubon:
Mail: 255 Sherman Hollow Rd, Huntington, VT 05462
Fax: 802-434-4686
E-mail: firstname.lastname@example.com
3. Send full payment by check to 'Audubon Vermont.' 255 Sherman Hollow Rd, Huntington, VT 05462
For more information, please contact:
Education Program Coordinator
Debbie Archer
(802) 434-3068
How you can help, right now
Support Audubon Vermont
Help us engage people of all ages in education, conservation and action. Audubon Members receive camp and program discounts as well as Audubon Magazine.
Raise Your Voice
Lawmakers need to hear from you. By signing up for Audubon Vermont's Action Alerts, you will get the latest news on proposals that impact birds -- and have an opportunity to weigh in.
Find Your Bird-Friendly Plants
Join Audubon's Plants for Birds. With our native plants database, you can easily find the best plants for the birds where you live.


text:
Homophobic crimes and offences against those who visit areas known for gay cruising are much more common than figures show, police believe.
PC Anton Brown, who works on issues affecting the lesbian, gay, bisexual and transgender community in Redbridge, says many victims are unwilling to report crimes such as robbery and sexual assault because they do not want their sexuality revealed.
He said: "Our research shows that thefts, robberies, rape and other violent assaults take place at these locations and the victims are scared to report them as they do not want to be 'outed'.
"They are often men that have sex with men and may not necessarily identify as being gay.
"We patrol these locations as research shows that members of public that frequent them are easy targets for crime.
"We estimate there to be three times as many crimes against LGBT that are not reported.
"Many of the initial reports we do get from victims or witnesses are screened out at the first stage because they do not want it to be followed up."
So far in 2013, there have been 22 reported crimes of this type in Redbridge, compared to 11 in 2011 and 17 in 2012.
"Hopefully this is an indication that the positive engagement work with the LGBT community at PSE's and other events such as crime stalls and surgeries, is increasing their confidence to report incidents to the police," PC Brown said.
Having sex in public is illegal, but police rarely make arrests.
"Whilst the practise is illegal, in most cases the police do not arrest or prosecute as the men that attend the locations do not engage in sexual activity in public view," PC Brown added.
"We try to engage with the members of the public and try to support them by pointing out the dangers."
Redbridge Police will be setting up an information stall on the issue between 10am and 12pm on January 23 at the cafe in Ray Lodge Park in Snakes Lane East, South Woodford.


text:
New transport group for Winchester district
Press Release: 02/09/2016
A new group to consider transport issues across the district is being set up by Winchester City Council.
The Winchester District Transport Board will be led by key members and officers and will focus on how to deliver the aims and objectives set out in the Council's Community Strategy.
It will also consider transport-related issues in Portfolio Holder Plans, strategies and policies and the delivery of major projects as well as enabling stakeholders to raise issues of concern.
The City Council hopes that public transport providers, transport policy-makers and other interest groups will form part of this new Board. In addition there will be opportunities for public input and engagement as work develops.
Cllr James Byrnes, Winchester City Council's Portfolio Holder for Transport and Professional Services, said:
We have considered carefully how the Council can best address transport issues not just in the city but across the whole district.
We believe the new Winchester District Transport Board will provide that focus and is particularly relevant now as the County and City Councils are about to start a study looking at traffic movement in and around Winchester.
Cllr Frank Pearson, Portfolio Holder for Environment, Health and Wellbeing, said:
Transport is very important to the Council and it has direct links to other issues we want to address such as air quality, encouraging walking and cycling and improving the public realm.
I look forward to working with partners to consider and address transport-related issues across the district.
The Winchester District Transport Board will be established this autumn and replaces the City Council's support for the Winchester District Strategic Partnership Transport Group which was a sub-group of the former Strategic Partnership. It will also take on the work previously covered by separate walking and cycling groups.


text:
afterpay now available...buy now. pay later.
What are your delivery options?
1. Standard Australian delivery, 2-5 business days, $7.95
2. Express Australian delivery, 1-2 business days, $9.95
3. Free Shipping for orders over $150 (Australia & New Zealand) Orders will be dispatched via Standard Shipping.
4. New Zealand delivery, 4-7 business days, $20.00
5. International delivery, 7-10 business days, $30.00
Please note that Australia Post & Express Post operates between all capital cities (except Darwin and in Perth CBD only) and some major centres. Outside metropolitan areas guaranteed service is to and from townships only. If your item is for an address outside the Express Post network, Australia Post use the fastest possible transport links, but it won't be covered by their Next Day Guarantee.
What is your order cut-off time?
Please allow up to 24 hours for us to process your order after it is placed as we often fulfill our online orders from multiple locations. Generally, if you place an order before 10am, it will be sent that afternoon. During sale periods, processing time is closer to 24 hours.
If you urgently need an order, please call or email us ASAP and we'll see what we can do!
Do you deliver to PO boxes?
How can I track my order?
If you're a customer with a login, you can track your order in the 'My Account' section of the Tuchuzy website. Alternatively, you can track your order directly using the tracking code that was sent in your shipping confirmation email.
If you can't find your tracking code, please call or email us.
Where is my order? It should have arrived by now.
Please use the links above to track your order.
If you're not home when a driver attempts delivery, they will take it to your closest post office. If they've done so, there will be a note in your mailbox letting you know which post office location they've taken it to.
Bear in mind that extreme weather conditions such as flooding and bushfires can often affect the postal service and in turn, receiving your parcel in our quoted time frame.
Tuchuzy will not take responsibility of loss or damaged items during transit. Please email us for any enquiries email@example.com
I'm probably not going to be home when my parcel is delivered. What should I do?
If there is a safe place for the driver to leave the parcel without a signature, just leave a comment when you check out to give your authority to leave it somewhere safe (you can be specific) without signing for it.
You may also want to have it delivered to your work instead.
Otherwise, it will go to your nearest post office if you're not around.
The delivery option I want isn't showing up. Why is that?
Be sure to refer to the delivery option explanations above to ensure your shipping address is eligible for that delivery option.
What countries do you ship to?
(Australia), Austria, Belgium, China, Denmark, Finland, France, Germany, Hong Kong, Hungary, Italy, Japan, Netherlands, Norway, Portugal, Ireland, Singapore, South Africa, South Korea, Sweden, Switzerland, United Arab Emirates, United Kingdom, United States of America.
If you do not live in one of these countries, please contact us and we may be able to make delivery arrangements for you at an extra cost.
How much is international delivery and how is it sent?
Delivery is a flat $30 (New Zealand is $20).
We use a DDU (Delivery Duty Unpaid) service, which means you simply pay an ex-tax price at the checkout and are subject to any relevant local duties and taxes once the parcel arrives at your country.
Delivery can take anywhere between 7-10 business days. If you'd like it faster, please call or email us and we may be able to provide a quote for a faster service.
Generally speaking, the closer your are to Australia and to a capital city, you closer you can expect the item to arrive in the quicker end of that delivery estimate.
How can I track my order?
If you're a customer with a login, you can track your order in the 'My Account' section of the Tuchuzy website. Alternatively, you can track your order directly using the tracking code that was sent in your shipping confirmation email.
Do you accept returns on international orders?
Yes, we do. If you'd like to return something, please contact us first for your RA (Return Authorisation) number, let us know what you're returning, if you would like an exchange, credit or refund, then fill out the returns form enclosed in your parcel.
If you would like an exchange, just place a new order online for the item you would like, send the unwanted item back and we will refund you.
Pack it up securely ad send it back to us within 21 days using a traceable post service.
We do not accept returns on sale items.
The last day for guaranteed delivery:
- Standard (interstate): Monday 14 December
- Standard (Sydney): Friday 18 December
- Express: Monday 21 December
Extended Christmas returns - Items purchased between the 11 December - 21 December can be returned to Tuchuzy HQ by 8 January 2018. For items purchased after this date, the normal 14 days returns policy of 14 days applies.
What is your returns policy?
Customer satisfaction is important to us! If you are unhappy with the merchandise you received for any reason, simply return it to us and we will happily provide a credit note or refund under the following conditions:
- Merchandise must be returned within 14 days of dispatch for Australian and New Zealand customers and 21 days for other countries.
- Merchandise must be in saleable condition with all tags attached
- Merchandise must not be worn, damaged, altered or washed
- You must contact us before returning the item to receive an RA number
- At this stage we do not offer exchanges on any items. If you would like a different size or item, you will need to follow the returns process for a refund and repurchase the new item.
- Sorry - sale items cannot be returned or exchanged unless deemed faulty.
For hygiene reasons, lingerie and earrings cannot be returned unless deemed faulty.
This policy does not affect your statutory rights.
How do I make a return?
1. Contact us to let us know what you would like to return, why and if you would like a credit note or refund. We will then provide you with an RA number.
2. Fill out your returns form
3. Ensure the garment is in original condition with tags attached
4. Package up your item securely and send back within 14 days using a traceable service
Do you cover the cost of returns?
Items are to be sent back to Tuchuzy at the cost of the customer.
Have you received my return?
Please allow up to 3 working days upon us receiving your parcel to process your return. We recommend you send your item back using a traceable service so you know when we've received it. Your can always contact us to double check.
Unfortunately Tuchuzy cannot be held responsible for parcels without tracking that have not been received back.
When should I expect my refund to clear?
Please allow up to 3 business days from the date we receive the package to process your refund. From there, the funds can take between 24-72 hours to clear.
PayPal refunds are instant once processed.
How long do credit notes last?
Credit notes will be valid up to 6 months from the issue date. If you'd like an extension, please call or email us.
Can items I buy online be returned in store?
Sorry, unfortunately all online orders will need to be returned to the head office.
How long do credit notes last?
Credit notes will be valid up to 6 months from the issue date. Gift vouchers are valid for 12 months from the issue date. If you'd like an extension, please call or email us.
What payment types do you accept?
Visa, Mastercard, American Express, PayPal and Afterpay.
What currency are all the prices in?
All items are in AUD$. All payments are processed in AUD$.
My credit card details aren't being accepted. Why is that?
Please check that:
- Your billing address is the same address associated with your credit card
- Your name appears exactly the same as on the card
- There are no spaces in your credit card number
If you are still experiencing difficulties, please check with your bank or financial institution. If problems continue, please call or email us.
Can I still order online if I don't have a credit card?
Tuchuzy accepts PayPal, which allows you to pay by instant bank transfer.
Can I use two coupon codes?
It depends on the offer. Please see the fine print on the offer banner or email. If you try and enter two coupon codes and it doesn't work, that offer doesn't accept any other offers in conjunction with it.
If I find an item cheaper elsewhere, will you price match?
Generally we can, however there are a few conditions that we have to consider first (i.e. if it's an international stockist and their currency is different). Just contact us and we can help you out.
Is it safe to use my credit card on this website?
We do our utmost to ensure that every credit card transaction is secure. We use a RapidSSL CA SSL certificate for extra security. This is a high-grade Encryption key (Camellia-256, 256 bit encryption key) that ensures your payment and person info remains secure during your payment. Your credit card info cannot be accessed by any third parties.
Are credit notes and gift vouchers redeemable both in store and online?
Online credit notes and gift vouchers are not valid in store, and can only be used online. Please note all online gift vouchers are non-refundable and are not redeemable for cash. Any items purchased using a gift voucher will be refunded as a voucher.
I'm having issues with your website. What do I do?
Please call or email us, or use our live chat during business hours and we will be happy to help get to the bottom of any issues for you.
For the best shopping experience, we recommend using Google Chrome, Apple Safari or Mozilla Firefox. Many websites are no longer designed to cater to browsers such as Internet Explorer.
Any screenshots you can take of error messages help us a great deal!
Why won't my login work?
Try a few different combinations and if you still have trouble, please reset your password or you can call us during business hours to confirm which email address you have registered with us.
My details have changed. How can I update them?
You can edit your details in the My Account section.
How do I know if my order has gone through?
All successful order transactions will receive an order confirmation email within 30 minutes. If you have not received your order confirmation email, check your junk or spam folder. Alternatively, log in to your account to check your order summary.
I think I made a mistake on my order. Can I update my delivery information?
Please contact us ASAP and we'll see if we can intercept it!
Why doesn't my coupon code work?
- Coupon codes are case-sensitive. So, if we've promoted the code in capital letters, you'll need to enter it in capital letters.
- Don't add spaces to the code
- Codes are often time sensitive – so if the offer is for 24 hours, we'll include a time when it will deactivate. If you're in a different time zone than AEST, this will affect the validity.
- Codes may not be able to be used more than once per customer
- You may only be able to use one code at a time with that particular offer.
If you still have problems, please contact us.
How can I unsubscribe from the newsletter?
You can do so from the bottom of any newsletter you have received from us. Would you like to follow us on Instagram, Facebook or Pinterest instead?
How should I launder my garment?
Please check the care instructions tab on the product page and follow the designers' instructions on the garment label.
The item I want is out of stock. Will you be getting it back?
Sometimes we have the ability to re-order hot sellers. Just contact us to find out.
Can I send something as a gift and can it be gift-wrapped?
You can make a note at the shopping cart to let us know it's a gift. We can include a special message, as we'll be sure to make it look special for you.
What is your mailing address?
Tuchuzy HQ / Online & Buying Office is located at:
Suite 1, G1, 292 Coward Street
Mascot, Sydney NSW 2020
1300 043 105
Tuchuzy Bondi Store is located at:
Shop 11, The Beach House
178 Campbell Pde, Bondi Beach, Sydney NSW 2026
1300 043 105
Can I send my resume to you?
We're always on the hunt for new talent. Please visit our Careers page to see our current vacancies or to send a general job enquiry.
I'm not sure what size to choose - help!
Just click on the 'size chart' link on the product page for international size conversions, or view the 'size & fit' tab to view product specific fit details. If you're still stuck, just contact us


text:
Murder Trial - Carrington Cottages Media Statement
In October 2014 there was an incident at Carrington Cottages boarding house in the city that resulted in the death of a man and the subsequent arrest of one of our tenants, Luke Ford. Mr Ford was charged with causing the death of the man. His trial for murder is at last set to commence on Monday 6 June 2016 in the SA Supreme Court.
The Chairman of the Carrington Cottages Board, Dr Bill Griffiths, said: "We are deeply saddened by this incident. The safety and security of our
tenants is, of course, of prime importance.
"Since the incident we have taken significant actions to upgrade and implement additional security measures in our boarding houses," he added.
Dr Griffiths advised that as the matter was now before the Court, neither he nor any staff or volunteers associated with Carrington Cottages were in a position to make further comment about the case.
For further information regarding the work of Carrington Cottages please contact:
Dr Bill Griffiths
Mobile 0488 057 660
Contact Profile
Dr Bill GriffithsP: 0488 057 660
W: www.carringtoncottages.org.au


text:
Yard Clean-Up
Protect your largest asset from fire, your home. I am a firefighter/paramedic and I know defensible space. Let us help you comply with your local codes and more importantly, protect your home in the event of wildfire. We use a Cat Skid steer with an industrial grade mower to clear your unwanted weeds.
Service areas include Tahoe/Carson/Minden/Reno/Sparks.
Call Bret Sanchez today for a free estimate. We are affordable, professional and guarantee your satisfaction.


text:
Photos are for members only!!
Peachmate is a 100% free sex site for adults looking for fun with no strings attached.
Escort ads, casual sex groups, personal ads, video chat and much more.
I enjoy trying new things. And new things along with most of the stuff that I've already tried was and will be fun.


text:
Todd Troeger- Vice Presiden at First Midwest Bank
A banking professional with more than 25 years of experience, Todd Troeger serves as vice president of commercial banking for First Midwest Bank. Since joining the Palos Heights, Illinois, financial institution in 2012, he has brought in more than $9 million in new assets and liabilities. Another of Todd Troeger's accomplishments during this period involved sourcing and submitting $65.5 million in new credits for underwriting.
Before accepting this role, Todd Troeger was vice president and relationship manager for Citibank in Tinley Park, Illinois. With the institution for a decade, he became its top development officer for two years and was named to its Circle of Excellence, an honor reserved for the top 10 percent of commercial bankers across the nation.
Earlier, Todd Troeger handled business development for SYM Financial Corporation in South Bend, Indiana. The recipient of the company's highest sales performance award one year, he created relationships and improved his firm's standing in areas ranging from 401(k) plan administration to wealth management services.
To prepare for his career, Troeger earned a bachelor of science in finance from Indiana University. He went on to acquire an MBA from Notre Dame.
The Four Point Play in Basketball
Todd Troeger spent a decade as vice president of Citibank. He spent two years in the same role with First Midwest Bank. In his free time Todd Troeger likes to play basketball and watch pro games on television.
In recent years the National Basketball Association (NBA) has been considering adding a four point line to the court. While the rule change may unnerve traditionalists, it should be noted that the three point line was not introduced until 1979. For now, however, basketball players can only score four points on a single shot if they are fouled while completing a three point attempt.
The Chicago Bulls' Sam Smith successfully completed the NBA's first-ever four point play in October of 1979, shortly after the establishment of the three point line. Facing the Milwaukee Bucks, Smith leaned into former teammate Brian Winters, the man who had taught Smith the move, while shooting a three. Winters was called for a foul as Smith's shot passed through the net. Smith sunk the free throw, though the Bulls would go on to lose the game 113-111.
In the nearly four decades that have passed since Smith's four point play no player has accomplished the maneuver more often than Jamal Crawford. He has successfully finished 44 four point plays over the course of his career. By comparison, as a franchise the Boston Celtics have only recorded 17 four point plays. JJ Redick of the Los Angeles Clippers finished the 2014-2015 with six completed four point plays on seven attempts to lead the league.


text:
Pomegranate Persimmon Salad With Warm Goat Cheese
READY IN: 25mins
Recipe by Maito
Very tasty! Worth the work of getting the pomegranate seeds out. You can use fuyu persimmons crunchy or soft, we prefer them at least a little soft.
Top Review by Thymestudio
I absolutely love this and I made it for a dinner party with my friends and got the most RAVE reviews! It was the hit of the weekend and I had to make everyone copies of the recipe to take home. I would give this 10 stars!!!!
See Discussion:10 Reviews
Ingredients Nutrition
- 1⁄2 cup pomegranate juice
- 2 tablespoons walnut oil or 2 tablespoons extra virgin olive oil
- 2 teaspoons balsamic vinegar (optional)
- 1 garlic clove, finely minced
- 1⁄2 tablespoon brown sugar, packed
- 2 -4 ounces soft fresh goat cheese
- fresh ground pepper
- 1⁄2 cup toasted walnuts, finely chopped
- 12 cups spinach or 12 cups other lettuce
- 2 fuyu persimmons, peeled and cut into wedges
- 1 pomegranate, seeded
- fresh basil (optional)
Directions See How It's Made
- Whisk together dressing ingredients.
- After salad ingredients are prepped, preheat oven to 375 degrees F.
- Form goat cheese into 1/2 oz. balls, top with freshly ground pepper, and roll in walnuts to coat. Place on a baking sheet.
- Assemble salad with greens, persimmons, pomegranate seeds, and basil.
- Just before serving, warm the goat cheese in the oven for about 3 minutes.
- Toss salad with dressing. Plate, carefully top with warm goat cheese, and enjoy!


text:
Please confirm that you want to add A Web Development Crash Course in HTML5 and CSS3 to your Wishlist.
HTML and CSS may seem intimidating, but there is a method to the madness. This course will show you everything that you need to know in order to make a functional and elegant website that suits your individual needs.
You do not need to have any programming experience to take this course. I will show you the basics, demonstrate examples, list common uses, and walk you through the development process step-by-step.
This course will cover:
You will also learn good web programming practice and debugging skills through practice and observation. This will make your projects easier for another developer to read and easier for you to read if you take a break.
Click Take This Course and learn how to make a website of your very own!
Let's make sure we have a solid understanding of HTML syntax before looking at the various HTML elements.
Let's see what you have learned so far.
JavaScript knowledge advised.
JavaScript knowledge advised.
JavaScript knowledge advised.
Derek has been a freelance Web Programmer for 8 years and is well-versed in HTML, CSS, JavaScript/jQuery, PHP, SQL, Java, and C# with a working understanding of C++ and ActionScript. He stays informed about current events and advancements in web technology and challenges himself to continue to use new advancements to drive creativity and user experience in his projects.
In his free time, Derek creates websites and web/mobile games. He is also beginning to use his understanding of programming to design functional microprocessors for small electrical devices and gadgets.


text:
Top 40 Chord Charts in July 2013
Featuring PraiseCharts Posted on July 23, 2013
Here are the top 40 chord chord from this month:
- Lord I Need You (Matt Maher)
- Christ Is Enough (Hillsong Live)
- Cornerstone (Hillsong Live)
- Whom Shall I Fear (God Of The Angel Armies) (Chris Tomlin)
- Oceans (Where Feet May Fail) (Hillsong United)
- In Christ Alone (Kristian Stanfill/Passion Band)
- 10000 Reasons (Bless The Lord) (Matt Redman/Passion Band)
- Man Of Sorrows (Hillsong Church)
- Great I Am (New Life Church)
- The Lord Our God (Kristian Stanfill/Passion Band)
- Glorious Ruins (Hillsong Live)
- Glorious (BJ Putnam)
- Jesus Only Jesus (Matt Redman/Passion Band)
- Redeemed (Big Daddy Weave)
- Our God (Chris Tomlin/Passion Band)
- From The Inside Out (Hillsong Church)
- One Thing Remains (Kristian Stanfill/Passion Band)
- Your Love Never Fails (Jesus Culture)
- Build Your Kingdom Here (Rend Collective Experiment)
- Amazing Grace (My Chains Are Gone) (Chris Tomlin)
- Here's My Heart (David Crowder/Passion Band)
- Victor's Crown (Darlene Zschech)
- Lord I Need You (Chris Tomlin/Passion Band)
- How Great Is Our God (Chris Tomlin)
- One Thing Remains (Bethel Live)
- Mighty To Save (Hillsong Church)
- Crown Him (with Majesty) (Chris Tomlin)
- Blessed Assurance (My King Is Coming) (Matthew West)
- I Will Follow (Chris Tomlin)
- This Is Amazing Grace (Bethel Live)
- Forever Reign (Hillsong Church)
- Your Great Name (Natalie Grant)
- How Great Thou Art (Paul Baloche)
- The Stand (Hillsong United)
- God's Great Dance Floor (Chris Tomlin/Passion Band)
- We Glorify Your Name (Hillsong Live)
- Whom Shall I Fear (God Of The Angel Armies) (Chris Tomlin/Passion Band)
- At Your Name (Phil Wickham)
- Love The Lord (Lincoln Brewster)
- Happy Day (Tim Hughes)
Other Posts Featuring PraiseCharts
- Free Chord Charts & Audio Tracks for Patriotic PraiseHymns
- A Quick Guide To Finding The Perfect Christmas Carol Arrangement
- Complete Your Christmas Planning With PraiseCarols
- Christmas Carols for Acoustic Worship
- The New PraiseCharts Is Here
- Survey - How Do You Use PraiseCharts?
- 2015 Winter SongQuest Winner Announced!
- Top 25 Worship Songs on the Holy Spirit for Pentecost 2015


text:
SE: Top Moments of October 2015
At the end of every month during the athletic and academic year, K-State Sports Extra glances back at the month's best plays, victories and moments. From exciting individual performances to teams pulling together for wins to facility dedications, October 2015 has been an exciting month to be a Wildcat.
Here are the moments K-State Sports Extra has dubbed the best of October 2015:
10. Former Wildcats shine in October
From Tyler Lockett's first career receiving touchdown in the NFL to Nicole Vogt making the roster of the U.S. women's bobsled team, it's been an exciting month for former Wildcats. The Seattle Seahawks' new return man, Lockett has also made an impact at wide receiver as he's recorded 17 catches for 217 yards and one touchdown in the Seahawks' first seven games.
On Oct. 27, Vogt (Rowing, 2005-10) was named to the 23-man roster for the U.S. bobsled team at the 2018 Winter Olympic Games. Vogt has been participating in bobsled since graduating K-State in 2010.
Along with Lockett and Vogt, Darren Sproles made headlines after returning a 45-yard punt for a touchdown in the Philadelphia Eagles' 23-20 last minute loss to Washington on Oct. 4. Taking a look overseas, Thomas Gipson (men's basketball, 2011-15) has helped Kouvt, a professional basketball team in Finland, to a strong 4-0 start and is averaging 16.8 points and 8.0 rebounds per game.
9. Rowing Solid in Jayhawk Jamboree
The Wildcat rowing squad closed out its fall competition at the Jayhawk Jamboree in Lawrence, Kansas, on October 18 where four of the Wildcats' boats – the Varsity 8, the Novice 8, the Wildcat A and the Wildcat B – earned top-five finishes at the event.
"It was a good finish to the fall season," head coach Patrick Sweeney said. "Now we will starting focusing on preparing for the spring season."
For more on the Wildcat rowing team, please click here, or to watch K-StateHD.TV's most recent rowing feature "Life as a Rower," please click here.
8. Lewis earns Preseason All-Big 12 Honors
K-State women's basketball junior forward Breanna Lewis was named to the Preseason All-Big 12 team on Oct. 7. The only Wildcat to receive the honor this season, it marks the first time in Lewis' career that she has been named to the preseason team and follows a stellar sophomore campaign where she broke the K-State record for blocks in a season with 97.
"It was very exciting, I wasn't expecting it at all," said Lewis about receiving the preseason recognition. "It's a great thing to have, and it gave me confidence to know that other people acknowledge that I am a good player, not only my coaches and teammates. Knowing other people think I am a player worthy of the preseason mention, it adds a lot of confidence."
To read K-State Sports Extra's recent feature on Lewis, please click here.
7. Wedekind posts another top-five finish
At the Bradley Classic in Peoria, Illinois, on October 16, the K-State women's cross country team finished in fourth place and was led by junior Morgan Wedekind's fifth-place finish, her third top-five finish of the season.
Wedekind finished the race with a time of 20:34. Prior to her fifth-place finish at the Bradley Classic, Wedekind notched a second-place finish at the Bob Timmons Classic in Lawrence, Kansas, and a fifth-place finish at the Cowboy Jamboree in Stillwater, Oklahoma.
6. Volleyball sweeps Oklahoma on road
K-State snapped a six-match losing streak in Norman, Oklahoma, on Friday, Oct. 16, as the Wildcats hit .366 and limited Oklahoma to its lowest attack percentage this season in a sweep of the Sooners (25-16, 25-21, 25-19) in McCasland Field House.
The victory for the Wildcats was their first sweep at Oklahoma since 2005 and first win of any kind in Norman since 2008.
To read more on the Wildcats' victory over the Sooners, please click here.
5. Kody Cook surprises all at Oklahoma State
When starting quarterback Joe Hubener was sidelined after taking a tough hit at Oklahoma State on October 3, Wildcat wide receiver Kody Cook came in to fill his shoes. Cook – K-State's fifth string quarterback – surged K-State to a 28-20 halftime lead over No. 20/19 Oklahoma State thank to throwing for 122 yards and two touchdowns on 10-of-16 passing, while he led the team in rushing with 87 yards and another score.
"(He) was very good. I thought he played with poise; he played smart," said K-State head coach Bill Snyder about Cook following the game.
Though the Wildcats' ended up losing a close, 36-34, contest in Stillwater, Cook's performance impressed and surprised many.
4. Tennis goes 7-0 on Final Day of SMU Fall Tournament
K-State women's tennis went undefeated on the final day of the SMU Fall Tournament on Oct. 11, earning five singles wins and two doubles victories to end the weekend with a 12-9 record. Head coach Danielle Steinberg was happy with the way her team performed.
"Today we got a little tougher as a team," said Steinberg. "It was the third day of the tournament, and everyone had tough battles yesterday, but we all showed a high level of maturity in how we competed."
To continue reading about the Wildcat women's tennis team, please click here.
3. Brand earns Big 12 Player of the Week Honors
After being the only player in the Big 12's top-10 for both blocks and digs per set for the week of October 18, K-State volleyball's Katie Brand was named the Big 12 Defensive Player of the Week on October 26.
Brand averaged 4.83 digs and 1.33 blocks over K-State's pair of matches against Texas and Texas Tech as she ranked third and tied for fifth, respectively, in the two categories. The setter had a total of 29 digs and eight blocks – along with 64 assists (third in the Big 12) and eight kills – over six sets.
The honor marks the second defensive weekly award of Brand's career and third overall.
To continue reading about Brand, please click here.
2. Kober earns Big 12 Player of the Week Honors
Following a career-high 37 digs at TCU on Saturday Oct. 3, K-State junior Kersten Kober earned her first-career Big 12 Defensive Player of the Week honor for the period ending October 4.
A native of Southlake, Texas, Kober was the first K-State libero to earn Big 12 Defensive Player of the Week honors since Lauren Mathewson in 2009. It was also the fifth time a Wildcat libero has earned the conference's top weekly defensive accolade, an award that started in 2007.
Her 37 digs marked the third-most in a match in program history – just four shy of tying the all-time record – and the most by a Wildcat since 1987.
To read K-State Sports Extra's recent feature about Kober, please click here.
1. Basketball Training Facility dedicated to Ice Family
Carl and Mary Ice gathered with their closest K-State friends and family to celebrate a very special occasion on Friday, October 16. K-State Athletics dedicated its basketball training facility to the passionate K-State family that evening, permanently renaming the building the Ice Family Basketball Center.
"It's difficult to describe our emotion; we're overwhelmed," said Carl, a 1979 K-State graduate in Industrial Engineering. "K-State has been an important place to us, it always has been. We've been blessed with the opportunity to help and never really expected that anything would happen to us directly."
To continue reading about Carl and Mary as well as the Ice Family Basketball Center dedication, please click here.
But wait, there's more!
Though we've shared K-State Sports Extra's Top 10 Moments, October is not quite over yet.
There is plenty of home action coming up for K-State fans to enjoy. The Wildcat equestrian team takes on Baylor beginning at 10 a.m., tomorrow morning at Timbercreek Stables followed by the opening exhibition for K-State men's basketball against Emporia State at 7 p.m., in Bramlage Coliseum. K-State volleyball returns to action in Ahearn Field house on Saturday at 7 p.m., to take on TCU, while the K-State cross country team is set to compete in the Big 12 Championship in Stillwater, Oklahoma, on Saturday morning.
Stay tuned to www.kstatesports.com or follow K-State Athletics on Twitter using @kstatesports or @kstate_gameday for updates on all of the Wildcats' events this weekend.