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This study investigates the role of the activated polymorphonuclear cell (APMN) products on sickle red blood cell (SRBC) retention/adherence in the pulmonary circulation. Isolated rat lungs were perfused with51Cr-labeled normal RBCs (NRBC) or SRBCs (10% hematocrit) suspensions ± PMNs. Specific activities of lung and perfusate were measured and retention (the number of SRBC/g lung) was calculated. SRBC retention was 3.5 times greater than NRBC retention. PMN activation was required to increase SRBC retention. Supernatants from APMN increased SRBC retention, which suggested soluble products such as oxidants, PAF, and/or leukotriene (LTB4) are involved. Heat inactivation of PMN NADPH oxidase had no effect on retention. Whereas neither platelet-activating factor (PAF) nor LTB4 (secreted by APMN) increased SRBC retention, PAF+LTB4 did. The PAF antagonist, WEB-2170, attenuated SRBC retention mediated by PAF+LTB4 and APMNs. Similarly, zileuton (5-lipoxygenase inhibitor) attenuated APMN-mediated SRBC retention. We conclude the concomitant release of PAF and LTB4 from APMN is involved in the initiation of microvascular occlusion by SRBCs in the perfused rat lung.
- platelet-activating factor
- leukotriene B4
- zileuton
central to microvascular occlusion in sickle cell disease (SCD) is the polymerization of deoxyhemoglobin S, which results in increased rigidity of the sickle red blood cell (SRBC). Because hemoglobin polymerization does not occur immediately after deoxygenation, most RBCs pass through the capillary bed before sickling, thus making microvascular occlusion uncommon (29, 50). On the basis of this observation, hemoglobin S polymerization is not likely the exclusive cause of microvascular occlusion and suggests that factors that increase the capillary transit time are also operative. Factors demonstrated in experimental models to increase transit time of the SRBC through the microcirculation are random precapillary obstruction by rigid dense SRBCs (contain irreversible SRBCs) and increased adhesion of sickle reticulocytes to vascular endothelium (37-39). Hebbel and colleagues (26) found that SRBCs, particularly reticulocytes, have an increased tendency to adhere to vascular endothelial cells (27) and that increased adherence can be correlated with clinical severity of vasoocclusion. Of the sickle reticulocytes, ∼25% express adhesion molecules, making them capable of adhering to vascular endothelial cells (35, 60). Regarding dense SRBCs, their correlation to clinical severity of vasoocclusion is less clear. Ballas (4) has described a subset of patients with sickle cell anemia who have mild disease, as related to pain, with increased numbers of rigid, dense SRBCs, leg ulcers, and lower mortality compared with patients with highly deformable SRBCs and low numbers of dense SRBCs. In contrast, Powars et al. (52) has shown that SCD patients with the most severe disease produce twice the number of dense SRBCs compared with patients with disease of minimal severity.
Nonetheless, dense SRBCs and reticulocytes are present during steady-state conditions and during clinical disease. This raises an interesting question as to whether or not other blood cells, such as polymorphonuclear cells (PMNs), play a role in the initiation of microvascular occlusion. Indeed, PMN activation has been implicated in the pathophysiology of SCD (44, 49). Several investigators (9, 18) have demonstrated that PMNs from some patients in crisis are more adherent to vascular endothelium than during steady state and possibly contribute to vasoocclusion. Clinical studies show that elevated total white blood cell counts are common in SCD (8,14) and that a white blood cell count >15,000 cells/ml is associated with an increased risk of early death (51). Cytokines such as interleukin-6 have been correlated positively with the number of sickle PMNs adherent to fibronectin (36). Interleukin-8 increases SRBC adherence to endothelial cells via fibronectin, upregulates α4β1-integrin receptors, and is a chemoattractant for PMNs (12, 3). These studies cumulatively suggest that PMN activation and the release of cytokines in response to infections, endothelial cell activation, and other injurious agents may prove to play a vital role in the pathophysiology of microvascular occlusion in SCD.
Ibe et al. (33) studied the interaction between SRBCs and autologous platelets in an isolated perfused lung model and suggested that lipid mediators may play a role in the pathophysiology of sickle lung disease. Lungs perfused with SRBCs and SRBCs plus autologous platelets revealed high perfusate levels of the arachidonate metabolites, thromboxane A2, and prostaglandin E2. Products of the arachidonate and 5-lipoxygenase were not measured. In our study, we propose that PMN activation, as may occur in response to infections or other injurious agents, increases SRBC retention/adherence in the pulmonary circulation of isolated perfused rat lung via a mechanism(s), which involve the release of platelet-activating factor (PAF) and leukotriene B4(LTB4) from the activated PMN.
Phorbol myristate acetate (PMA) and bovine serum albumin (BSA; 66,000 mol wt) were purchased from Sigma (St. Louis, MO). PAF and LTB4 were purchased from Cayman Chemical (Ann Arbor, MI). Zileuton was purchased from the University of South Alabama Pharmacy (Mobile, AL). WEB-2170 base was a kind gift from Boehringer Ingelheim Pharmaceuticals. PMA, WEB-2170, and PAF were dissolved in DMSO. LTB4 and zileuton were dissolved in 95% ethanol.
Isolated Perfused Lung
Male Sprague-Dawley rats (275–350 g) were anesthetized with nembutal sodium (25 mg ip), and lungs were removed for extracorporeal perfusion as previously described (25, 47). A tracheostomy was performed that permitted ventilation with a Harvard rodent ventilator (model 683) at 55 breaths/min with a tidal volume of 2.5 ml and 2.0 cmH2O-positive end-expiratory pressure. The inspired gas mixture was 21% O2-5% CO2-balance N2 (room air gas). A median sternotomy was performed, heparin sodium (100 IU) was injected in the right ventricle, and cannulas were placed in the pulmonary artery and left ventricle. Heart, lungs, and mediastinal structures were removed en bloc and placed into a humidified chamber. Lungs were perfused with the use of a Gilson Minipuls 2 peristaltic pump at a constant flow of 0.03 ml · g body wt−1 · min−1. Lungs were perfused with a physiological salt solution (PSS) containing bovine serum albumin (BSA). The PSS-BSA perfusate contained (in mM) 119 NaCl, 4.7 KCl, 1.17 MgSO4, 22.5 NaHCO3, 1.18 KH2PO4, 3.2 CaCl2, 5.5 glucose, and 4 g/100 ml BSA. PSS-BSA (100 ml) was perfused through the lungs in a nonrecirculating fashion to remove residual blood cells and plasma. The perfusate was then changed to a PSS-BSA perfusate that contained RBCs from individuals homozygous for sickle cell anemia. The perfusate hematocrit was ∼10%. Pulmonary arterial pressure and pulmonary venous pressure were continuously monitored with pressure transducers (model 041–500–503, Cope) and recorded on a polygraph recorder (model 7E, Grass Instruments). Zone 3 flow conditions (arterial > venous > alveolar pressures) were maintained throughout all experiments.
Blood Collection
All participants gave informed consent and the Human Use Committee approved the protocol. During steady state, blood samples (∼40 ml) were obtained from normal (Hemoglobin A) human volunteers and from individuals with homozygous sickle cell anemia in the sickle cell clinics at the University of South Alabama. Samples were collected in syringes containing heparin sodium and utilized within 48 h of collection. Individuals who had received blood transfusions within the previous 6 wk were excluded from this study. Sickle cell anemia was documented by hemoglobin electrophoresis in each subject.
RBC Isolation and 51Cr Labeling
Each whole blood sample was centrifuged at 4,100 rpm for 10 min, followed by the removal of the plasma and buffy coat. Packed RBCs were then mixed at a 1:3 dilution with normal saline and washed three times. With each wash, the RBC suspension was centrifuged for 10 min at 3,000 rpm, followed by removal of the supernatant. After the third wash, complete removal of platelets and PMNs was verified by microscopic examination of stained smears. Packed RBCs were resuspended in phosphate-buffered saline (PBS; 1 ml PBS and 1 ml packed RBCs) and incubated with 100 μCi 51Cr for 60 min in a shaker water bath at 37°C. After incubation, the 51Cr-labeled RBCs were washed once with normal saline and resuspended in PSS-BSA to obtain a hematocrit of ∼10%.
Neutrophil Isolations
Heparinized whole blood (20 ml) from individuals with homozygous sickle cell anemia was diluted 1:1 with normal saline. PMNs were then isolated after dextran sedimentation and centrifugation on histopaque 1077 cushions as described previously (10). Briefly, dextran 500 was added to the 1:1 mix at one-fourth the volume of the 1:1 mix. This was allowed to incubate for 45 min at 37°C. The supernatant (contains PMNs) was aspirated into a 50-ml centrifuge tube and underlayered with 10-ml histopague 1077. This was centrifuged at 1,250 rpm for 30 min. The supernatant was aspirated and discarded, leaving the PMN pellet. Sterile H2O (3 ml) was added to the PMN pellet and agitated for 30 s to lyse any residual RBCs. PBS (45 ml) was added immediately thereafter to stop the reaction. This was centrifuged at 1,250 rpm for 10 min. The supernatant was aspirated and discarded. This process of RBC lysis, followed by PBS, was repeated until the PMN pellet was without visual evidence of RBC contamination. The PMN pellet was suspended in 1 ml of sterile Hanks' balanced salt solution (HBSS). A total PMN count was obtained using the Cell-DYN 900 Coulter counter. Final preparations contained 95% PMN and viability was >95% as assessed by trypan blue exclusion.
Heat inactivation of neutrophils.
Purified PMNs, suspended in 1 ml of sterile HBSS (pH 7.4), were transferred to a siliconized glass tube and placed in a 48°C water bath with constant agitation for 10 min (17, 21). Aliquots of the heat-treated PMN suspension were immediately added to a 10% sickle RBC suspension to obtain a final PMN concentration of ∼200,000/ml of perfusate.
RBC Retention
Cell counts of the 51Cr-labeled RBC suspension were obtained in a Cell-DYN 900 Coulter counter. The specific activity of the 51Cr-labeled RBC perfusate was calculated after gamma counts at 162–176 Kev for 1 min and expressed as counts per minute (cpm)/RBC. The isolated lung was then perfused with the51Cr-labeled RBC perfusate for 30 min. After this, the lung was washed with 100 ml of RBC-free PSS-BSA under identical hemodynamic conditions. Perfusion was stopped, and the lung was dissected into lobes and weighed. Each lobe was gamma counted for 1 min. Retention (R) was calculated as In the isolated perfused lung, the increased retention of SRBCs can occur from increased adhesion to vascular endothelium or secondary to mechanical obstruction. Thus throughout the text the number of retained SRBCs per gram per lung is referred to as retention/adherence.
Specific Protocols
Comparison of normal RBC and SRBC retention/adherence in the isolated lung.
Initially, studies were done in the isolated rat lung perfused with 10% hematocrit RBC suspensions, which contained either SRBCs (n = 8) or normal human (Hemoglobin A) RBCs (NRBC;n = 6). Lungs were ventilated with a room air gas mixture, as described earlier in methods. After a 30-min period of perfusion with the RBC-containing perfusates, SRBC and NRBC retention/adherence was determined as previously described and compared.
Comparison of unactivated PMN to activated PMN effect(s) on SRBC retention/adherence.
Subsequently, in isolated SRBC-perfused rat lungs ventilated with a 21% O2-5% CO2- balance N2 gas mixture, the effects of PMN activation with PMA on altering SRBC retention in the pulmonary circulation were assessed. All perfusate hematocrits were ∼10% and paired SRBC samples were utilized in each study except the study comparing NRBC and SRBC retention. Paired SRBC samples were used to minimize patient-to-patient variability as well as within-patient variability relative to time. In studies utilizing PMNs, PMNs were added to the perfusate at ∼200,000/ml of perfusate. The activation of PMNs was achieved in the test tube by the addition of PMA (20 ng/ml) to PMNs suspended in 1 ml of HBSS. Activated polymorphonuclear cells (APMNs) were then added to the perfusate reservoir at the initiation of lung perfusion in experiments, which investigated SRBC and APMN interactions. Lungs were perfused in a recirculating fashion under constant flow conditions for 30 min and the retention/adherence of SRBCs was determined as previously described. The following comparisons of SRBC retention relative to PMN activation were made: 1) SRBC versus SRBC + unactivated PMNs (UPMN; n = 6 each); 2) SRBC + UPMN versus SRBC + APMN (n = 11 each); 3) SRBC + APMN versus SRBC + APMN supernatant (n= 8 each); and 4) SRBC versus SRBC + PMA (n = 9 each).
The comparison of lungs perfused with SRBC + APMNs versus SRBC + APMN supernatant suggested either a soluble product(s) of the APMN and/or mechanical impedence by entrapped APMNs increased SRBC retention/adherence in the pulmonary circulation. PMN activation results in stimulation of NADPH oxidase and superoxide production (2, 13) and in the production of phospholipid products (7, 34).
Heat inactivation of PMN NADPH oxidase: effect on SRBC retention/adherence.
To assess the potential role of NADPH oxidase, the following studies were performed. Purified PMNs were heated at 48°C for 10 min to inactivate NADPH oxidase (17, 21). In lungs perfused with51Cr-labeled SRBCs, retention/adherence was assessed in the presence of heat-treated APMNs (∼200,000/ml perfusate) versus APMN control (no heat treatment).
Effect of PAF and LTB4 on SRBC retention/adherence.
The phospholipid products assessed in this study were PAF and LTB4. To assess the role of PAF (100 ng/ml) and LTB4 (100 ng/ml) in SRBC retention/adherence, the following groups were compared: 1) SRBC versus SRBC + PAF 30 (PAF added to perfusate reservoir at the initiation of lung perfusion and allowed to recirculate for 30 min; n = 6 each);2) SRBC versus SRBC + LTB4(n = 5 each); 3) SRBC versus SRBC + PAF + LTB4 (n = 9 each); 4) SRBC + PAF + LTB4 versus SRBC + WEB-2170 (PAF antagonist) pretreatment + PAF + LTB4(n = 8 each); 5) SRBC + APMN versus WEB-2170 pretreatment + SRBC + APMN (n = 6 each); and 6) SRBC + APMN versus SRBC + zileuton (5-lipoxygenase inhibitor) pretreated APMN (n = 8 each). In group 2, LTB4 was added to the perfusate at the initiation of perfusion. In group 3, PAF was added to the reservoir at the initiation of perfusion and allowed to recirculate for 30 min. LTB4 was then added to the perfusate for an additional 30 min of lung perfusion. In group 4, WEB-2170 (10 μM final perfusate concentration) was added to the perfusate reservoir at the initiation of perfusion and allowed to recirculate for 20 min before PAF administration and 50 min before the administration of LTB4. This protocol was the same as ingroup 3, except for the 20-min pretreatment of lungs with vehicle or WEB-2170. In group 5, WEB-2170 (10 μM) (19, 28, 56) was added to the SRBC perfusate 20 min before the addition of APMNs (∼200,000/ml perfusate). In the study using zileuton, PMNs were incubated with zileuton (2.5 μM) for 30 min before activation with PMA in the test tube. Zileuton (2.5 μM) was used based on the finding reported by Guidot et al. (20) and preliminary studies performed in our laboratory, which demonstrated an ∼50% inhibition of plasma LTB4 production in A-23187-stimulated whole blood from individuals with homozygous sickle cell anemia. APMNs were subsequently added to the perfusate reservoir at the initiation of lung perfusion. Lungs were perfused in a recirculating fashion for 30 min and the SRBC retention/adherence was determined as previously described.
All results are presented as means ± SE. Statistical analyses were performed using the unpaired Student's t-test and one-way analysis of variance (ANOVA). Tukey's test (62) was used for multiple comparisons when ANOVA indicated statistically significant differences between or within groups. Differences were considered to be significant whenP < 0.05.
SRBC Retention/Adherence in Isolated Perfused Lung is Greater Than NRBC
When comparing RBC retention in lungs perfused with SRBCs with lungs perfused with NRBCs, we found that SRBC retention was 3.5 times greater than that observed in NRBC-perfused lungs (Table1).
PMN Activation is Required to Increase SRBC Retention/Adherence
In lungs perfused with SRBCs alone compared with lungs perfused with a SRBC suspension that contained UPMNs, no significant increase in retention/adherence in the lung was observed in the presence of UPMNs. In lungs perfused with SRBCs alone and in lungs perfused with SRBC + UPMN, the number of retained SRBCs as expressed in RBC/g of lung was 3.9 ± 0.3 × 108 and 4.3 ± 0.4 × 108, respectively. In SRBC-perfused lungs with a perfusate that contained either UPMN or APMN, the presence of APMN resulted in a 31% increase in retention/adherence in the pulmonary circulation (Table 1). From Table 1, data were pooled from lungs perfused with SRBCs alone (n = 23), SRBC + UPMN (n = 17), and SRBC + APMN (n = 19), and compared. SRBC retention/adherence was 4.6 ± 0.2 × 108, 4.3 ± 0.2 × 108, and 5.9 ± 0.3 × 108 SRBC/g of lung, respectively. As reported using paired SRBC samples (see specific protocol), there was no significant difference in SRBC retention in lungs perfused with SRBC alone and SRBC + UPMN. SRBC retention in lungs perfused with SRBC + APMN was also significantly (P < 0.001) greater than that seen in the SRBC alone and SRBC + UPMN groups.
Supernatant From APMN Increases SRBC Retention/Adherence
To determine whether the increase in retention/adherence of SRBCs seen in the presence of APMN was due to the release of a product(s) of the APMN, SRBC retention/adherence in lungs perfused with SRBC + APMN was compared with lungs perfused with SRBC + the supernatant of APMN. No significant difference in SRBC retention/adherence was noted in SRBC perfused lungs that contained APMN and supernatant from APMNs.
PMA Had No Effect on SRBC Retention/Adherence
Because all of the perfusates containing PMA and phorbol esters have been reported (43) to activate the α4β1-integrins on the surface of sickle reticulocytes and promote increased adherence of the reticulocyte to endothelial cells via fibronectin, SRBC retention/adherence in lungs perfused with SRBC alone was compared with lungs perfused with SRBC + PMA. Lungs perfused with PMA did not significantly increase SRBC retention/adherence (Table 1). To further assess whether PMA increased SRBC retention/adherence in the lung, or whether it was a product of the APMN, lungs perfused with SRBC + UPMN were compared with a lung perfused with SRBC + washed APMN (PMNs were activated with PMA then washed before adding to the perfusate, which contained SRBCs). Washed APMN caused a 19% (P = 0.033) increase in SRBC retention/adherence compared with SRBC + UPMN-perfused lungs.
Heat Inactivation of PMN NADPH Oxidase Had No Effect on SRBC Retention/Adherence
The potential role of PMA activation of PMN NADPH oxidase with superoxide production in SRBC retention/adherence was assessed next. When we compared lungs perfused with SRBC + APMN and SRBC + heat-treated APMNs, no significant (P = 0.885) difference in SRBC retention/adherence was observed between groups. In SRBC-perfused lungs with APMN, retention/adherence was 4.5 ± 0.4 × 108 SRBC/g of lung and 3.9 ± 0.4 × 108 SRBC/g of lung in lungs perfused with heat-treated APMN (n = 9 each).
PAF +LTB4 in Tandem is Required to Increase SRBC Retention/Adherence
The effect(s) of PAF and LTB4, which are soluble products of the APMN, was assessed (Fig.1-5). In lungs perfused with SRBC alone compared with SRBC + PAF for 30 min, retention/adherence was 5.0 ± 0.7 × 108 and 4.1 ± 0.6 × 108 SRBC/g of lung, respectively. No significant difference in SRBC retention/adherence was observed (Fig. 1). When comparing SRBC retention/adherence in control lungs (5.1 ± 0.6 × 108 SRBC/g of lung) to lungs perfused with SRBC + LTB4 (4.7 ± 0.4 × 108 SRBC/g of lung) for 30 min (n = 5 each), there was no significant difference in SRBC retention/adherence (Fig.2). Interestingly, in lungs perfused with SRBC + PAF for 30 min, followed by the addition of LTB4 to the perfusate for an additional 30 min, there was a significant (P = 0.02) increase in SRBC retention/adherence compared with SRBC control (n = 9, each, Fig. 3). SRBC retention/adherence in the SRBC control and SRBC + PAF + LTB4 groups was 4.4 ± 0.2 × 108 and 5.5 ± 0.2 × 108 SRBC/g of lung, respectively.
WEB-2170 and Zileuton Attenuate APMN-Mediated SRBC Retention/Adherence
To further assess the role of PAF and LTB4 as potential mediators of PMN-enhanced SRBC retention/adherence, the effects of the PAF antagonist, WEB-2170 (28, 61) and the 5-LO enzyme inhibitor zileuton (6, 20) were assessed. When we compared SRBC retention/adherence in lungs perfused with SRBC + PAF + LTB4 with lungs perfused with SRBC + PAF + LTB4, which were pretreated with WEB-2170, SRBC retention/adherence was 4.9 ± 0.5 × 108 and 3.3 ± 0.5 × 108, respectively. Pretreatment with WEB-2170 resulted in a 33% decrease in SRBC retention/adherence compared with SRBC + PAF + LTB4-perfused lungs (Fig. 4). Comparison of SRBC retention/adherence in lungs perfused with SRBC + APMN ± pretreatment with WEB-2170 resulted in SRBC retention/adherences of 5.9 ± 0.1 × 108 and 4.8 ± 0.3 × 108, respectively (P = 0.0028). WEB-2170 pretreatment resulted in a 19% decrease in SRBC retention/adherence compared with the SRBC + APMN control. SRBC retention was then assessed in lungs perfused with SRBC + APMN and compared with lungs perfused with SRBC + PMN, which were pretreated with zileuton before PMA activation. In the SRBC + APMN-perfused lungs, SRBC retention/adherence was 5.95 ± 0.6 × 108compared with 3.40 ± 0.3 × 108 in the SRBC + zileuton + APMN group. Zileuton pretreatment resulted in a 43% decrease in SRBC retention/adherence compared with the SRBC + APMN control (Fig. 5).
The concentration of PMNs in pulmonary capillary blood is 40–80 times higher than seen in the blood of large vessels (5, 15). Many individuals with SCD have chronically elevated peripheral white blood cell counts in the absence of an acute crisis, which suggest the marginal pool of PMNs in the pulmonary circulation may be higher than that reported in normals (8,14). Boghossian et al. (9) and Fadlon et al. (18) have demonstrated that PMNs from patients in crisis are more adherent than control PMNs and suggest that enhanced PMN adhesion to vascular endothelium could contribute to the initiation of vasoocclusion in the acute crisis of SCD. However, we postulate in this study that PMN activation, as seen with inflammation, infection (57), or possibly in relation to SRBC adherence to PMNs (30), initiates vaso-occlusion via the release of phospholipid products, which increase SRBC retention/adherence in the pulmonary circulation.
In this study, the major findings are the following: 1) SRBC retention/adherence is 3.5 times greater than that seen in lungs perfused with NRBCs; 2) for PMNs to increase SRBC retention/adherence in the pulmonary circulation, PMN activation is required; 3) increased SRBC retention/adherence is not due to PMA; and 4) PAF in tandem with LTB4production from the APMN are at least partly responsible for the increased pulmonary microvascular retention/adherence of SRBCs observed in this study.
Pulmonary microvascular occlusion by abnormally adherent and/or nondeformable SRBCs has been reported (1, 25) as a potential factor in the initiation of acute lung injury. Similar to this study, which demonstrated SRBC retention/adherence to be 3.5 times greater than that seen with NRBCs in lungs ventilated with a room air gas mixture, Aldrich et al. (1) reported a threefold increase in SRBC retention compared with NRBCs in the isolated rat lung ventilated with 95% O2. This study further demonstrated that regional ventilation with 0% O2 resulted in a 25-fold increase in SRBCs compared with NRBCs. Unlike the study by Aldrich et al. (1), which reported that the retention of SRBCs in pulmonary microvasculature was primarily due to the mechanical entrapment of nondeformable SRBCs, our study provides data supporting a nonmechanical mechanism, which involves the release of PAF and LTB4 from the APMN in the increased retention/adherence seen in the isolated rat lung model.
The requirement of PMN activation to increase SRBC retention/adherence supports our hypothesis that PMN-SRBC-endothelial cell interactions may initiate vasoocclusion. However, this finding did not allow for distinguishing between mechanical properties of the APMN and/or the release of a soluble product(s), which could contribute to the observed increase in SRBC retention. Activation of the PMN in response to inflammatory mediators such as interleukin-8, C5a, PAF, LTB4, tumor necrosis factor-α,N-formylmethionyl-leucyl-phenylalanine, or PMA causes an increase in PMN f-actin, which results in decreased PMN deformability and increased endothelial cell adhesion (15,22-24, 42). The same mediators cause neutropenia and pulmonary microvascular PMN sequestration when infused intravascularly (15). Decreased PMN deformability, increased PMN adhesion to vascular endothelium and the larger PMN size relative to the RBC (15, 22-24, 42) are likely important determinants of increased microvascular resistance. Each of these PMN properties could prolong RBC transit time through the pulmonary microcirculation and contribute to the increased SRBC retention/adherence observed in this study (31, 42). To exclude the potential contribution of these biomechanical properties of the APMN in increased SRBC retention, studies that compared SRBC retention in lungs perfused with APMNs to lungs perfused with the supernatant of APMNs were performed. In these studies, SRBC retention was essentially identical, which strongly suggested that in part the product of the activated PMN was responsible for increased SRBC retention in the pulmonary circulation. However, before this could be concluded, an additional study investigating a potential role of PMA on the upregulation of adhesion molecules on the sickle reticulocytes and/or endothelial cells was performed. Kumar et al. (43) reported that phorbol esters activate the α4β1 integrins on the surface of sickle reticulocytes and increase the adherence of the reticulocyte to endothelial cells via fibronectin. In contrast, incubation of endothelial cells with the phorbol esters did not increase adhesion of sickle reticulocytes. The authors concluded that phorbol esters increase sickle reticulocyte adhesion to endothelium via activation of α4β1 integrins and this effect was independent of endothelial cell vascular adhesion molecule-1 (VCAM-1). In our study, PMA added to the perfusate did not increase SRBC retention when compared with SRBC control. This finding excludes any significant role of the integrin receptor α4β1 or endothelial cell vascular adhesion molecule-1 in the observed increased SRBC retention seen.
Beyond the biomechanical changes seen with PMN activation, a series of enzymes [phospholipases C (PLC), D (PLD), and A2(PLA2)] involved in the generation of phospholipid products by the PMN and NADPH oxidase is activated (2). Of particular interest are oxidants and the products of membrane phospholipids and PLA2, lyso-PAF-acether (when acetylated results in PAF), and free arachidonic acid (AA) (2,11, 58).
Mild heating of human PMNs inactivates NADPH oxidase. This inactivation of NADPH oxidase produces a defect in superoxide production without altering PMN adhesion or chemotaxis (17, 21). In this study, heat-treated APMNs did not result in a decreased SRBC retention compared with the APMN control. This suggested that APMN-related increased SRBC retention/adherence was not due to NADPH oxidase activation. This led to further studies, which examined the role of PAF and LTB4 as products released by the APMN, which could increase SRBC retention in the pulmonary circulation.
AA, lyso-PAF-acether, and other metabolites are released from the membrane phospholipids of stimulated PMNs by PLC, PLD, and PLA2 (2, 7). The major product of AA, which is secreted by human PMNs, is the 5-lipoxygenase product LTB4(20). The lyso-PAF-acether is inactive, but when acetylated by an acetyltransferase, its metabolically active product PAF is formed (7, 58). Both PAF and LTB4 can cause the release of other inflammatory mediators, and influence vascular permeability, cell infiltration, and PMN adhesion to vascular endothelium (7, 45). Perhaps a similar interaction between PAF and LTB4 on SRBC-endothelial interaction also occurs. Interestingly, PAF priming of human umbilical vein endothelial cells increases LTB4-mediated PMN adhesion (46). The LTB4 effect on PMN-endothelial cell adhesion is dose dependent with a maximum at 1 μM, requires no more than 1 min of preincubation, and decays linearly over 30 min once LTB4 is removed (32). There are no data currently available that describe LTB4 having any effects on SRBC-endothelial cell interactions. Our study supports that LTB4 alone has no effect on SRBC retention/adherence in the rat lung circulation. In contrast, there are data demonstrating that PAF increases SBC adhesion to rat ex vivo mesocecum vasculature through a mechanism that involves PAF induction of endothelial αVβ3. This effect is blocked by monoclonal antibodies to αVβ3 (40). In addition, Sultana et al. (59) reported that the PAF receptor antagonist CV-3988 inhibits CoCl and hypoxia-induced SRBC adhesion to vascular endothelium. Whereas these data using CV-3988 provide indirect evidence that PAF plays a role in increasing SRBC adhesivity to vascular endothelium, they alone do not exclude the potential interaction of PAF and LTB4 in tandem as proposed in this study. In our study, PAF alone did not increase SRBC retention/adherence. Possible explanations for why no effect of PAF on SRBC retention/adherence was seen are: 1) the time course was too long, resulting in the metabolism of PAF and a loss of the transient period of hyperadhesiveness; 2) the51Cr-labeled SRBC assay was not sensitive enough to demonstrate lower degrees of increased SRBC retention/adherence; and3) PAF alone does not increase SRBC retention/adherence in the rat lung circulation.
Whereas the rheology of the APMN has been studied, the effects of the release of its phospholipid mediators PAF + LTB4, on SRBC retention/adherence in the pulmonary circulation has not. In the present study, lungs perfused with PAF and LTB4 in tandem increased the retention of SRBCs in the pulmonary circulation compared with control. Increased SRBC retention/adherence was partially reversed compared with controls in lungs perfused with PAF + LTB4 + WEB-2170 (PAF antagonist), and in lungs pretreated with WEB-2170 before the addition of APMNs. The decrease in SRBC retention/adherence seen in the PAF + LTB4 + WEB-2170 group compared with the PAF + LTB4 control paralleled studies utilizing LTB4 alone. Whereas PMN activation results in the release of PAF and LTB4 and APMNs increase SRBC retention/adherence, lungs pretreated with WEB-2170 before the addition of APMNs attenuated APMN-mediated SRBC retention/adherence. Similarly, in lungs where PMNs were pretreated with the 5-lipoxygenase inhibitor zileuton before activation with PMA, decreased SRBC retention/adherence was observed compared with APMN controls. 5-lipoxygenase inhibition with zileuton results in decreased production of LTB4 (6, 16), which is the principal leukotriene released by human PMN (20). These observations with WEB-2170 and zileuton support the hypothesis that both PAF and LTB4 are required to increase SRBC retention and link the APMN to the SRBC-endothelium interactions reported in this study. The decrease in SRBC retention/adherence seen with WEB-2170 caused a 33% decrease in SRBC retention/adherence in PAF + LTB4-treated lungs and zileuton treatment of PMNs before activation decreased PMN-mediated SRBC retention/adherence 43%. In additional studies (n = 4), combination treatments of WEB-2170 (in perfusate) and zileuton pretreatment of PMNs before activation significantly decreased APMN-mediated SRBC retention/adherence by 45% but did not demonstrate synergism compared with WEB-2170 or zileuton treatments alone (data not shown). These findings, coupled with those demonstrating that neither PAF nor LTB4 when individually perfused increased SRBC retention in the pulmonary circulation, provide convincing evidence that APMN secretion of PAF and LTB4 in tandem is at least partly responsible for the observed increase in SRBC retention/adherence seen in the pulmonary circulation of the isolated-perfused rat lung model. Although these findings have not been reported previously, Setty et al. (55) have reported in a static incubation system that AA metabolites are involved in mediating basal adhesion of normal and SRBCs to endothelium via lipoxygenase metabolites. In subsequent studies, Setty et al. (53, 54) found that SRBCs stimulate endothelial cell production of AA and diacylglycerol and that the lipoxygenase product 12(s)-hydroxy-5,8,10,14-eicosatetraenoic acid increased hypoxia-induced SRBC-endothelial adherence via the upregulation of VCAM-1 on endothelial cells. These studies are similar to the present study in that products of membrane phospholipids, related in part to the lipoxygenase enzyme system, appear to be involved in mediating SRBC retention/adherence. These studies differ in that Setty et al. analyzed RBC-endothelial cell interaction in a static system while this study investigated PMN-SRBC-endothelial cell interactions in a dynamic flow model. Further studies will be required to determine whether the primary effect of PAF + LTB4is on the endothelium or SRBC.
In conclusion, our results support a potential role of the APMN in the initiation of pulmonary microvascular occlusion, which involves the phospholipid, proinflammatory mediators, PAF and LTB4. This observation possibly explains how PMN activation in response to infections or other injurious insults, such as that seen in reperfusion injury, precipitates vasoocclusion in SCD and the acute chest syndrome. Furthermore, it supports potential therapeutic approaches employing PAF antagonist and/or 5-lipoxygenase inhibitors in the prevention of SRBC-endothelium interactions.
We thank Marilyn Chancellor for the preparation of this manuscript and Dr. Betty Pace for reviewing this manuscript.
This study was supported by the National Heart, Lung, and Blood Institute Comprehensive Sickle Cell Program Grant P60 HL-38639 and the Florence Foundation Research Career Development Grant.
Address for reprint requests and other correspondence: J. Haynes Jr., Univ. of South Alabama Medical Center, 10th Fl., Suite H, 2451 Fillingim St., Mobile, AL 36617 (E-mail:jhaynes{at}usamail.usouthal.edu).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2002 the American Physiological Society


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Hi there!
Go Heat??
Is this not the most ridiculous suit you've ever read about -- this guy has been capturing University of Alabama football players in various states of triumph and exaltation, and for this he gets a Lanham Act and trademark suit:
Since 1979, Daniel A. Moore has painted famous football scenes involving the University of Alabama (the "University" or "Alabama"). The paintings feature realistic portrayals of the University's uniforms, including helmets, jerseys, and crimson and white colors. Moore has reproduced his paintings as prints and calendars, as well as on mugs and other articles.This serves the guy right for venerating 'Bama, I guess.
In 2002, the University told Moore that he would need permission to depict the University's uniforms because they are trademarks. Moore contended that he did not need permission because the uniforms were being used to realistically portray historical events. The parties could not reach a resolution, and in March 2005, the University sued Moore in the Northern District of Alabama for breach of contract, trademark infringement, and unfair competition.
No good deed goes unpunished.
I blame Nick Saban.

subset: commoncrawl


text: Swift Energy Company (NYSE: SFY) ("Swift Energy") and PT Saka Energi Indonesia ("Saka") announced that U.S. subsidiaries of Swift Energy and Saka have executed definitive agreements to fully develop approximately 8,300 acres of Fasken area Eagle Ford shale properties owned by Swift Energy in Webb County, Texas. Closing is anticipated on or about June 30, 2014, subject to normal closing conditions. The executed agreements, effective January 1, 2014, provide for Saka to pay Swift Energy $175 million in total cash consideration to acquire a 36% full participating interest in Swift Energy's Fasken properties. The consideration is comprised of $125 million in cash to be paid at closing and $50 million in cash to be paid by Saka to carry a portion of Swift Energy's future field development costs. Swift Energy will continue to serve as operator of the Fasken properties, conducting all drilling, completion and production operations, with development plans for the field to be mutually agreed upon by Swift Energy and Saka as provided for in the definitive agreements. J.P. Morgan Securities LLC acted as financial advisor to Swift Energy in this transaction. "Saka Energi is the perfect partner to jointly develop this acreage alongside Swift Energy," Terry Swift, CEO of Swift Energy commented. "Saka recognizes the opportunity in the Fasken area to create value through highly productive natural gas drilling and is aligned with Swift Energy in its assessment of the valuation of our Fasken acreage. Both parties are committed to rapidly realize the full value of this asset through an aggressive, disciplined development program. "From Swift Energy's perspective, this transaction also allows us to reduce financial leverage in the near term and maintain capital spending levels that will support growth in 2015. The impact of additional drilling activity in 2014 made possible by this transaction is reflected in revised guidance issued by us today in a separate release.


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Today is 90210 day, how awesome is that?! And when I realized it is September 2, 2010, thereby meaning it is 90210, I got giddy like a kid on Christmas! One of the hottest TV shows of my generation (which transcends generations), is the original Beverly Hills 90210. To this day, I watch 90210 everyday on Soap Net. And then I hear the same comments being made by Tori Spelling about spending the weekends in bed with Donna Martin. That could be taken out of context, but it is mostly about laying around all weekend watching 90210. Sounds amazing to me!
In honor of 90210 day, we have a collage of the original cast (present day photos) chilling by the Peach Pit. The only gal who we cover because of her handbags is Tori Spelling. Tori is a Balenciaga girl, which we completely appreciate.
Share with us your favorite 90210 character and episode!
P.S. Please consider supporting our small, bag-loving team by clicking our links before shopping or checking out at your favorite online retailers like Amazon, Neiman Marcus, Nordstrom, or any of the listed partners on our shop page. We truly appreciate your support!


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Brain Locates Source of a Sound With Temporal, Not Spatial, Clues
By SANDRA BLAKESLEE
Published: May 10, 1994
ROAMING the woods on a lovely spring afternoon, a birdwatcher hears the tat-tat-tat of a woodpecker at work. His head turns toward the sound, which came from the left, probably near that big oak tree 50 yards away. Yes, there it is -- a downy woodpecker.
Localizing interesting sounds is an ordinary human experience, but to scientists who study the human auditory system, the feat is mind-boggling. The woodpecker emits sound waves that travel through air, enter human ears and set off a chain reaction of impulses and computations that result in a person's being able to locate the bird's position, instantly and accurately. How this occurs remains cloaked in mystery.
Last week a researcher in Florida turned up a new clue. Although his finding does not solve the larger puzzle, it does show that the auditory system has evolved coding strategies that work differently from other sensory systems. Moreover, the auditory cortex -- the higher brain region where sounds are interpreted and understood -- contains a population of cells that appear to be unlike any others in the nervous system.
The researcher, Dr. John C. Middlebrooks in the department of neuroscience at the University of Florida Brain Institute in Gainesville, published his work in the current issue of Science magazine.
These special cells in the cortex can detect sounds occurring anywhere in the 360 degrees of space around the human body, Dr. Middlebrooks said. But they have developed a special code for differentiating the location of these sounds. For example, if a sound comes from, say, 10 feet to the left of a person's head, the cell will fire its signals in one pattern. "It might go dit, dit, ditty, dit," Dr. Middlebrooks said. But if the sound originates from a different point in space, the cell fires another pattern, he said, perhaps "ditty ditty dit." All sounds from all locations can be coded in this way by these cells, he said.
"It's remarkable to show that a single neuron can encode sounds from every direction in space," said Dr. Eric Knudson, a professor of neurobiology at Stanford University medical school. It means that these auditory cells operate according to rules that are different from those governing other brain cells that help make sense of the outside world.
But the finding does not solve the question of how humans localize sounds, Dr. Knudson said. Although these cells produce a code, he said, it is not clear how or if the brain uses the signals in sensory processes. They could be passed on to other brain circuits to help understand sounds, Dr. Knudson said, or they could be an artifact of other processes.
The brain contains internal "maps" or representations of space that help people understand the world, Dr. Knudson said. Each sensory system -- seeing, hearing, touching and so on -- has specialized neurons that help create these maps, which are constantly being acted upon and re-created as humans react to the external world. (In dreams, the same cells are activated, but not by the outside world -- the brain generates its own signals from within and tries to make sense of them.)
The neurons that perform these mapping functions have what are called receptive fields -- a limited part of space to which they react. For example, the visual system contains cells that detect only horizontal lines. Others fire only when exposed to vertical lines. A neuron with a receptive field for horizontal lines will not fire signals to other neurons when it encounters vertical or diagonal lines or other basic shapes. It is activated only by horizontal lines -- like the edges of a desk.
There are cells that specialize in color, motion, where the head is located in space and where the body is located in space, Dr. Knudson said. Some help determine what an object is and others help decide where it is. Motor System's Maps
Similar detailed maps exist for the somatosensory system -- there are cells that tell people where their body parts are located. And there are maps for the motor system -- cells that help calculate the trajectory of a baseball flying toward a person's hand.
All these maps are spatial representations of the outside world, said Dr. Knudson. These specialized cells, with their small receptive fields, then pass their mapped information up through various networks to the cortex -- a thin layer of cells at the top of the brain where advanced processing takes place. Then, through intricate connections, the information is passed back down the same networks for the final act of cognition or perception.
This model has worked so well that researchers thought there should be spatial maps in the auditory system, Dr. Middlebrooks said. And, in fact, there are. The inner ear maps the frequency, timing and loudness of sounds. These signals are then sent to the mid-brain where processing begins to determine sound location. Basically, the brain compares the intensity and timing of sounds received in each ear. If a sound arrives at one ear before the other, the time delay is used to help calculate where the sound is coming from. People with uneven hearing loss may have trouble, for this reason, locating sounds.


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After the week my family and I have had, these quotes fit perfectly. You sure learn who is there for you to lean on, without saying a word, when your world as you know it falls apart. I have had a shitty week to remind myself of this, but have made it through with the help of very good friends and a few select family members. You all know who you are, thank you.
Real friendship is shown in times of trouble; prosperity is full of friends. -Ralph Waldo Emerson


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As a school girl growing up Gujarat, my schools in Ahmedabad and Vadodra taught me to revere Mahatma Gandhi: he is father of the nation, he got us our freedom and was the most beloved of all Indian leaders. I never questioned that; but nor did I give it much thought.
As a college student in Pune, I encountered a very different point of view on Gandhi – for the first time I heard of his name being taken with contempt by some of my fellow students. Those students felt that what Nathuram Godse did that dark day of January in 1948, was justified; even necessary. I was appalled, I was amazed and I was indignant.
This is the time when my interest in Gandhiji was genuinely piqued; the time that I read up about the man and realised what a truly tremendous soul he was and why. I first did this for a self-centered reason; so I could effectively counter the arguments of others with divergent views on Gandhi. Soon it was a profound admiration for the man and his methods that spurred my actions.
Gandhiji showed us the difference between freedom fighting and terrorism
He immortalised the quote "an eye for an eye makes the whole world blind". He not only said this; this was a credo he lived by. He demonstrated that when you face your oppressor with bombs and bullets you reduce yourself to his level; you are no better than your oppressor, no matter how just your cause or how lofty your endeavour.
I will offend and outrage people when I say this, but forming armies to attack the British, murdering police officers, and hurling bombs inside the Central Legislative Assembly while shouting Inquilab Zindabad are ignoble. These acts may be brave; may even be spurred by a fierce national pride, but then you could say the same about Kashmiri youth committing acts of terror in the name of 'Azadi' today. Modern suicide bombers maybe a little unhinged but they are certainly brave!
Mahatma Gandhi showed us the tremendous power of non violence. The helpless rage of the British empire in the face of his passive resistance meant that our erstwhile oppressors did not know quite how to react to this man's utterly unique tactics. There was no precedent and British could not clamp down on this form of resistance with their usual brutality – the non violent resistance simply did not justify it.
He not only showed the Indian freedom movement the way; he was able to unite India in a manner unprecedented before or after. He had the sort of iron resolve that made the largest empire on earth bow to his wishes. He was able to halt communal riots simply by refusing to eat.
Gandhiji was a genuinely simple man/h3>
Pandit Nehru once famously said about Gandhiji's steadfastly frugal ways: "keeping him in poverty costs a lot of money". Seeing Gandhi Ashram on the banks of the Sabarmati River in Ahmedabad, reveals what a completely Spartan life the man led. He had very little and showed the world that it was possible to survive on very little. His habit of using the charkha to make his own clothes set a remarkable example of self-reliance that each of us, stuck in a morass of material desires and ambitions could learn from.
Mahatma Gandhi has inspired generations of world leaders
Gandhiji was arguably the most influential leader of the 20th century. If there is one Indian leader who genuinely makes us proud – who really taught the world a thing or two, it is Mahatma Gandhi. Now, 65 years after his assassination he continues to inspire world leaders with his singular ideas.
American civil rights leader Martin Luther King drew inspiration from Gandhiji's methods for his own struggle; so did Nelson Mandela in this struggle against Apartheid in South Africa. Barrack Obama also claims to be inspired by this small giant of a man, as do Aung San Suu Kyi of Burma and Benigno Aquino of the Philippines.
Mahatma Gandhi and his ideals are just as relevant today
His principles of non-violence, truth, religious harmony and tolerance are timeless; they are his legacy to the world. It is a different matter that most Indians now consider Gandhi and his legacy an anachronism; his ideas as inapplicable to modern reality.
Most people are content to place a photo of the man on a wall and then promptly forget all about him and his ideals. It would be foolish and shortsighted to relegate the man and his ideas to history… Gandhiji's ideals are as relevant today as they always were; they are eternal. We Indians can ignore them at our own peril.
Image Source: See page for author [Public domain], via Wikimedia Commons


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Thread: Borland BDE 5.01
12-11-13, 08:59 #1Registered User
- Join Date
- Feb 2006
- Posts
- 26
Unanswered: Borland BDE 5.01
We bought a software back in 2011, and it's using BDE 5.01. I'm having errors, "Table is Full", and unable to enter data.
After googling, I need to increase the "Block Size" in BDE. Then need to create new tables, move the data to new tables, then rename the new tables to original names.
Since it's using BDE 5.01, does it mean Paradox 5.01? I'm worried about version conflict once I start converting.
Or, is there a better way to fix this issue?We're just a speck of dust in a universe.
12-11-13, 10:08 #2Registered User
- Join Date
- Feb 2006
- Posts
- 26
Need help getting hold of Paradox 5, I can't find it anywhere. It can be trial version, I just need to convert the database files.
Thanks.We're just a speck of dust in a universe.


text:
- published: 01 May 2015
- views: 65365
In ethics, value denotes something's degree of importance, with the aim of determining what action of life is best to do or live (deontology), or to describe the significance of different actions (axiology). It may be described as treating actions themselves as abstract objects, putting value to them. It deals with right conduct and good life, in the sense that a highly, or at least relatively highly, valuable action may be regarded as ethically "good" (adjective sense), and an action of low, or at least relatively low, value may be regarded as "bad".
What makes an action valuable may in turn depend on the ethic values of the objects it increases, decreases or alters. An object with "ethic value" may be termed an "ethic or philosophic good" (noun sense).
Values can be defined as broad preferences concerning appropriate courses of action or outcomes. As such, values reflect a person's sense of right and wrong or what "ought" to be. "Equal rights for all", "Excellence deserves admiration", and "People should be treated with respect and dignity" are representative of values. Values tend to influence attitudes and behavior. Types of values include ethical/moral values, doctrinal/ideological (religious, political) values, social values, and aesthetic values. It is debated whether some values that are not clearly physiologically determined, such as altruism, are intrinsic, and whether some, such as acquisitiveness, should be classified as vices or virtues.
What are your core values and are you using them to improve the quality of your life and your work? Part of discovering what values exist at your core is looking back at the failures and triumphs of life to improve and ensure a successful future. James Franklin is approaching his second season as the head coach of Penn State's Football program. Franklin was named the 16th head football coach in the storied 127-year history of the Nittany Lion program on January 11, 2014. A two-time All-Pennsylvania State Athletic Conference (PSAC) quarterback at East Stroudsburg University, Franklin has demonstrated the ability to recruit, teach and motivate talented student-athletes throughout his coaching tenure. Franklin graduated from East Stroudsburg in 1995 with a degree in psychology. He also earne...
What is the most important thing in life? Money? Happiness? Love? Those things are certainly important, but what matters most is good values. What are values? They are what we consider more important than our feelings. For instance, just about everyone feels like eating junk food, but if you eat whatever you feel like eating you will end up obese and unhealthy. So then, what stops people from eating all the food they feel like eating? The answer is good values. Indeed a lack of good values is the root of virtually everything wrong with the world. In five minutes, learn why we should act based on values rather than our feelings. You can support PragerU by clicking https://www.classy.org/checkout/donation?eid=60079 Free videos are great, but to continue producing high-quality content, contr...
Will getting that great job or house really make you happier? In this fun & entertaining video, Dr. Russ Harris, Acceptance & Commitment Therapist, explains the important distinction between living a goals-focused vs a values-focused life... and why living in accordance with your innermost values can lead to a more rich, full, and meaningful life. To learn more about Dr. Harris's new & exciting online Acceptance and Commitment Therapy training for mental health professionals and therapists, visit http://www.imlearningact.com today! p.s. Subscribe to our channel and be the first to know when we upload another fun and educational video on Acceptance and Commitment Therapy... http://www.youtube.com/channel/UC-sMFszAaa7C9poytIAmBvA
Join Life Mastery Accelerator: http://lifemasteryaccelerator.com/ In this video, I share with you an exercise on how to determine your core life values.Through this process, we are going to discover whether your current values are propelling you forward, or holding you back. Our values form the foundation of our lives; they determine the decisions that we make and the direction that our lives take. In the words of John C. Maxwell, "Your core values are the deeply held beliefs that authentically describe your soul." The first step is to acknowledge and take responsibility for the life that you have created. If your values aren't in alignment with your vision, there will be resistance, in the form of a tremendous amount of push, effort, and willpower. Alternatively, when you align with w...
Music recorded, mixed and mastered by Harwood Records. Video produced by Judd Wrighton. Facebook - www.facebook.com/valuesofficialuk Bandcamp - www.valuesofficialuk.bandcamp.com Twitter - www.twitter.com/values_official Instagram - Valuesofficial_uk Lyrics: I can't stand to hear another word, It's changing me Why should I sit here and be denounced, In such a way Defense to attack in the blink of an eye. No moment of respite You fail to see what's right and wrong in this world. As if the borders collapsing Change, it changes me It changes me (It changes me) If you lived like this yourself. You'd see You just don't understand And you never will And now when I look at you I don't know what to think, I don't know how to make you see who you are I DONT KNOW WHAT TO FUCKING SAY Anymore! W...
KARA SWISHER: What do you imagine the next ten of your life will be about? STEVE JOBS: Um. You know. This is probably a bad example, but I'm going to use it. When this whole thing with Gizmodo happened. I got a lot of advice from people that said, you gotta just let it slide. You can't, uh, you shouldn't go after a journalist because they bought stolen property, and they tried to extort you. You should let it slide. Apple's a big company now, you don't want the PR. You should let it slide. And I thought deeply about this, and I ended up concluding that the worst thing that could possibly happen as we get big, and we get a little more influence in the world is if we change our core values and start letting it slide. I can't do that. I'd rather quit. You know, you go back five years ag...
Website: http://www.Yourworldwithin.com Facebook: https://www.facebook.com/Yourworldwithin Twitter: https://www.twitter.com/eddiepinero2 Instagram: https://www.instagram.com/eddiepinero2 Motivational Video - Value Created at Your World Within Speech by Eddie Pinero Music: Time Stops by Silent Partner For speaking Inquiries: http://www.yourworldwithin.com/hire-me-to-speak/ All copyrights go to their respective owners. Your World Within does not own the rights to these video clips. They have, in accordance with fair use, been repurposed with the intent of educating and inspiring others. However, if any content owners would like their images removed, please contact us by email at email@example.com. Check out YourWorldWithin.com for more videos, articles, and community discussion
In the spirit of ideas worth spreading, TEDx is a program of local, self-organized events that bring people together to share a TED-like experience. At a TEDx event, TEDTalks video and live speakers combine to spark deep discussion and connection in a small group. These local, self-organized events are branded TEDx, where x = independently organized TED event. The TED Conference provides general guidance for the TEDx program, but individual TEDx events are self-organized.* (*Subject to certain rules and regulations)
Have you ever felt so confused, because you had so many ideas and options and you didn't know which one was the best one to choose? Discovering your core values will really help you to get clearer and make decisions which align better with who you are. Read more here: http://femaleentrepreneurassociation.com/2012/12/3-steps-to-figuring-out-your-core-values/
Provides definitions and examples for the concepts of values, beliefs and attitudes.
I wish I could say everything you used to be blows up in smoke,
for everybody else to see it's a joke,
doesnt't't it make you feel like you have to choke,
on the words you spoke,
when the promise you broke.
You were looking mighty small to me,
cought up in your world of misery.
Cause when you found a light to lead you the way,
you were looking for a fire to lead you astray.
*Out of your mind, you were looking for change
you were going blind.
Say what you want, be what you want, be who you wanna be!
You held your values so high, how could you sink so low?
You tried to reach for the sky, now youve got nowhere to go.
You think it's worth the pain, you say you're free to choose.
Hey, youve got nothing to gain but everything to lose!
I don't understand why you had to sacrifice your ideals,
everithing you stood for, I know how it feels.
Bouncing back and forth day by day,
never reaching home never wanted to stay.
You held your values high, too hgh to reach,
you almost touched the sky, now you're back on your feet.
Follow the light again, leading the way,
and spit out the fire that lead you astray.
You held your values high, too high for you to keep
You almost touched the sky, now you're back on your feet
You had your plans and hopes, you had your dreams to share


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|Please note: This article is about the Miya Herald. For other uses of the term, please see Iko (disambiguation).
Miya Iko was a Miya Herald who in 1167 was sent by Miya Shoin to interview the nezumi Chief of Chiefs of the One Tribe, Kan'ok'ticheck. Iko told him she was a Shisa, a collector of stories to be told and remembered. Kan'ok'ticheck appointed personal bodyguards to the Rokugani Rememberer, and Iko was gifted with a fetish that offered potent protection from dark magic. Iko told the Imperial stories to the nezumi chieftain, who listened to them carefully. A comparable reception was made by the One Tribe to newer visitors that bore the Miya mon. [1]
- ↑ News of Rokugan (Imperial Herald v2#18)
|This Imperial Families related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


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Roasted Corn and Chicken Tostadas
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READY IN: 40mins
Recipe by ElizabethKnicely
From specialk.com
See Discussion:
Ingredients Nutrition
- 1 (7 inch) whole wheat flour tortillas or 1 (7 inch) flour tortillas
- nonstick cooking spray
- 1⁄3 cup frozen whole kernel corn
- 1 teaspoon vegetable oil
- 4 ounces boneless skinless chicken breasts, cut into bite-size pieces
- 1⁄2 teaspoon chili powder
- 1⁄4 teaspoon ground cumin
- 1⁄4 cup salsa
- 1⁄4 cup canned black beans, rinsed and drained
- 1 cup shredded lettuce
- 2 tablespoons shredded reduced-fat cheddar cheese
- 1 tablespoon nonfat sour cream
Directions See How It's Made
- Lightly coat both sides of tortilla with cooking spray. Place on baking sheet. Bake at 400°F about 10 minutes or until crisp.
- Meanwhile, in small nonstick skillet coated with cooking spray cook corn over medium-high heat for 1 to 3 minutes or until beginning to brown, stirring frequently. Remove from skillet. Set aside.
- In same skillet heat oil. Add chicken, chili powder and cumin. Cook and stir over medium-high heat for 2-3 minutes or until chicken is no longer pink. Stir in salsa, beans and corn. Heat through.
- Place tortilla on serving plate. Top with lettuce, chicken mixture, cheese ad sour cream. Serve immediately.
- -1 container (6 ounce) low-fat lemon yogurt topped with 1/3 cup raspberries or blueberries and 1 tablespoon reduced-fat whipped topping.
- -1/2 cup orange juice mixed with 1/2 to 1 cup sparkling water.
- (Total Calories: 270).


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I usually include good and interesting posts in a full "Worth reading" post, but those are usually new posts. These are from 2009 and they are excellent and deserve one post of their own:
- When allies fail – Part One – What Tami Said
- When allies fail – Pt. 2 – What Tami Said
Tami is giving advice on how to be an ally to marginalised groups in a feminist setting. She is specifically talking about race, but what she writes translates to other groups too.
Allies are important to any equality movement. It does not help people of color if we are the only ones who understand racism and how it still exists in society. It does not help women if we are the only ones that believe we deserve equal treatment. This is especially true considering the ways that women and people of color have been kept from places of power. The battles are ours to fight, and we can win them, but we need allies.
There is a bunch of points with excellent advice in those two posts. Here is one example:
Allow us our anger. It isn't easy being a member of a marginalized group. For instance, I have written before about the dull aches of racism. I have also written about how members of marginalized groups are expected to hold their tongues in the face of mistreatment–to be the "bigger persons." What may seem like a very small deal to you, to us may be yet another wearying and soul-destroying slight. Any human being has a right to be angry about injustice. Again, this does not mean that we have the right to dehumanize or insult you. It is not an ally's job to be endlessly flogged and called to account for the sins of all society. But marginalized people do have a right to be pissed off and to show it.
Anyway, go read them. So much good stuff there. As a white person with my own privileges, there's plenty of advice for me too.


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Floating Ripple Blanket by Ellen Gormley
Welcome! You are browsing as a guestWould you like to see 10 projects made from this pattern and much more? create a free account What am I missing?
© Ellen Gormley
© Heather
Floating Ripple Blanket
This pattern is available for $5.00.
You choose the size, you choose any worsted yarns in any colors. Make it solid, make it scrap, make it striped! It's up to you.


text: Editor's Note: Any reference to TheStreet Ratings and its underlying recommendation does not reflect the opinion of TheStreet, Inc. or any of its contributors including Jim Cramer or Stephanie Link. All three major indices are trading up today with the Dow Jones Industrial Average ( ^DJI) trading up 12 points (0.1%) at 14,943 as of Thursday, Sept. 5, 2013, 12:50 PM ET. The NYSE advances/declines ratio sits at 1,432 issues advancing vs. 1,453 declining with 120 unchanged. The Services sector currently sits up 0.6% versus the S&P 500, which is up 0.2%. On the negative front, top decliners within the sector include H&R Block ( HRB), down 1.8%, Expedia ( EXPE), down 1.8%, Liberty Global ( LBTYK), down 1.2%, Home Depot ( HD), down 1.0% and Luxottica Group ( LUX), down 0.8%. Top gainers within the sector include Louisiana-Pacific ( LPX), up 11.1%, Sears Holdings Corporation ( SHLD), up 4.4%, Pandora Media ( P), up 3.2%, GameStop ( GME), up 3.1% and Burger King Worldwide ( BKW), up 2.8%. TheStreet would like to highlight 5 stocks pushing the sector lower today: 5. Amerco ( UHAL) is one of the companies pushing the Services sector lower today. As of noon trading, Amerco is down $4.13 (-2.5%) to $163.10 on heavy volume. Thus far, 52,789 shares of Amerco exchanged hands as compared to its average daily volume of 54,300 shares. The stock has ranged in price between $162.97-$167.50 after having opened the day at $167.49 as compared to the previous trading day's close of $167.23. AMERCO operates as a do-it-yourself moving and storage operator for household and commercial goods in the United States and Canada. Amerco has a market cap of $3.3 billion and is part of the diversified services industry. Shares are up 31.7% year to date as of the close of trading on Wednesday. Currently there is 1 analyst that rates Amerco a buy, no analysts rate it a sell, and 1 rates it a hold. TheStreet Ratings rates Amerco as a buy. The company's strengths can be seen in multiple areas, such as its revenue growth, notable return on equity, attractive valuation levels, solid stock price performance and impressive record of earnings per share growth. We feel these strengths outweigh the fact that the company has had generally high debt management risk by most measures that we evaluated. Get the full Amerco Ratings Report now. STOCKS TO BUY: TheStreet Quant Ratings has identified a handful of stocks that can potentially TRIPLE in the next 12-months. Learn more.


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and its sunday monday
MAX damage posting
i need to go to the shop later for tea and stuff but it's cold and rainy and i think starving to death is going to be easier
Think of that chicken doner kebab, dripping so much grease its coming through the polystyrene container. mmmmmmmmm
both stir fry and takeaway does sound good but i need things for lunch tomorrow as well when im working in the middle of nowhere and i could leave earlier and buy it in the morning but that means waking up earlier and thats a horrible idea
chilli con carne made EAT
My Attorney has advised me to remove my new signature until further notice...
one of my mates ate so much takeaway he could get the food on tic and the owner would come and personally deliver it to his house, before the place even delivered (was round the corner tbf). Were on first name terms and he would often come and chill for 10 minutes
Same mate also had some fat burning pills in his bathroom. Makes you think
My Attorney has advised me to remove my new signature until further notice...
You're right... thinking baked potato thoughts.
I had pulled pork at blues smokehouse so I can't really talk.
Avoided the burger cuz of the carbs tho so yeah.
then proceeded to coat it all in 3 different bbq sauces.


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Michael Page Kuwait
The Role
Leading Regional Bank seeks a Consumer Banking Chief Marketing Officer to be based in Kuwait
Responsible for leading the Banks Marketing activities, the Consumer Banks Products & Customer Segments and the Market Research & Business Analyses / MIS. Lead and direct marketing, products & segments activities to ensure the Bank's improvement in brand health and brand awareness, increase in market share across products and segments and alignment with its financial and strategic objectives and its products and services are known, understood and desired in the market place.
Job Offer:
Competitive for right candidate
15 – 18 years in commercial / marketing roles with exposure to financial services. At least 10 years strategic marketing & consumer banking product & segment leadership experience in the banking sector.
Kuwaiti nationals hugely preferred.
About the Company
Michael Page is one of the world\'s leading professional recruitment consultancies, specializing in the placement of candidates in permanent, contract, temporary and interim positions with clients around the world.
The Group has operations in the UK, Continental Europe, Asia-Pacific and the Americas. In the Middle East we focus on the areas of:
Finance & Accounting Banking & Financial Services Procurement Property & Construction Engineering & Supply Chain Oil & Gas Technical and Engineering Human Resources Sales Marketing Technology Secretarial Executive Search Legal
The Group operates through 161 offices in 33 countries and employs over 5,000 employees worldwide.


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Onsen Tamago (Hot Spring Eggs) are poached or soft cooked eggs in warm water. They have a silky custardy consistency delicious eaten with rice or noodles.
Onsen Tamago (Hot Spring Eggs)
Ais Kacang Gula Melaka
Ais Kacang Gula Melaka (Shaved Ice with Beans and Palm Sugar Syrup) – a Malaysian dessert with a medley of toppings. Very cool and refreshing on a warm day.
Pepperoni Flatbread Pizza
This Pepperoni Flatbread Pizza makes an easy light meal or snack. Can be prepared in minutes using prepackaged ingredients and some fresh produce.
Strawberry Nectarine Salsa
This Strawberry Nectarine Salsa is deliciously sweet, fruity, and a little addicting. Great served with seafood or as a dip with corn chips.
Mango Peach Salsa
Sweet, tangy, refreshing Mango Peach Salsa. Great with tortilla and corn chips for all your summer parties and get-togethers.
Cilantro Lime Chicken Rice
This easy to cook one-skillet, lightly spiced, zesty Cilantro Lime Chicken Rice is sure to please. It will quickly become a family favorite.
Meatball Noodle Soup
This warm and delicious Meatball Noodle Soup is comfort in a bowl. Very easy to prepare and perfect for cold winter evenings. Plus $100 Giveaway.
Yang Chow Fried Rice and Egg Roll Platter
Yang Chow Fried Rice, a popular fried rice cooked with ham, shrimps, carrots, green peas, and eggs. Delicious served with Pagoda egg rolls.
Apple Crisp with Crystallized Ginger
Easy to make Apple Crisp with Crystallized Ginger, cinnamon bread, and walnuts. Deliciously crisp and warmly spiced. Best ever dessert for this time of the year!
Cheesy Meatball Tater Tot Casserole
This Cheesy Meatball Tater Tot Casserole is comfort food for the meat and potato lover. It is warm, delicious, satisfying, and so easy to prepare.
Rosemary Thyme Baked Chicken
Rosemary Thyme Baked Chicken with a golden crispy skin. Only a few ingredients and minimum prep time. Great for any day of the week.
Slow Cooker Pulled Pork Sandwiches
Easy Slow Cooker Pulled Pork Sandwiches flavored with home blended spice mix. Minimum preparation time and can feed a crowd. Great for tailgate parties.
Slow Cooker Chili Dogs and Chili Corn Chips
Slow Cooker Chili Dogs and Chili Corn Chips – cook once and enjoy it both ways. Perfect for a tailgate party or potluck.
Tomato, Ham, and Potato Skillet
This hearty and delicious Tomato, Ham, and Potato Skillet is a great way to use up homegrown or store bought cherry tomatoes. Makes a delicious weekend breakfast or brunch.
Watermelon Lime Cooler
This super refreshing Watermelon Lime Cooler is a great way to beat the heat on hot summer days. Seltzer or club soda and lime make it sparkle.
Slow Cooker Chicken Stew
This Slow Cooker Chicken Stew is an all season comfort food complete with carrots, celery, and potatoes. Delicious with crusty bread or rice.
Cucumber Mint Cooler
Cucumber Mint Cooler topped with your choice of club soda, seltzer, or water, and a squeeze of lime juice. It's cool, fizzy, and super refreshing.
Sugar Snap Peas and Shrimps Stir Fry
Tender crisp Sugar Snap Peas and Shrimps Stir Fry in less than 30 minutes. Great for busy weeknights and perfect with a bowl of steamed rice.
Broccoli, Cauliflower, and Sausage Stir Fry
Broccoli, Cauliflower, and Sausage Stir Fry – super quick and tasty with easily available ingredients. Perfect for weeknights.
Sticky Hoisin Honey Chicken
Best ever Sticky Hoisin Honey Chicken with a sweet savory glaze that is sure to please. Very easy to prepare using only a few simple ingredients.
Sausage, Potato, and Egg Skillet
Wake up to this delicious Sausage, Potato, and Egg Skillet topped with shredded cheese and green onions. Makes a wonderful weekend breakfast or brunch treat for the family.
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Anyone who owns a GMC knows that when it comes to maintaining their automobile only the highest quality replacement parts will do. GMC automobile drivers have come to expect a certain level of reliability when driving their G1500 around town. It's not a regular occurrence that you discover you need a replacement Drive & Serpentine Belt to fix your vehicle. Obviously, you bought your G1500 because you understood you would be getting horsepower and great value both impeccably combined into one vehicle.
One of the keys to maintaining an operational engine and full use of your accessories is keeping an eye on your drive belts. Your GMC G1500 drive or serpentine belts transmit power from the crankshaft to these accessories. But since they are traveling at such high speeds, they can be prone to breaking; potentially leaving your engine without the use of its alternator, power steering, or more. GMC G1500 engines with just one belt that joins all of the accessories to the crank pulley have a serpentine belt, while more than one would all be called drive belts.
Buying new GMC parts from www.carpartsdiscount.com means you are paying the lowest prices for the highest quality original and aftermarket replacement G1500 parts on the internet. Buy all the car parts you will ever need from carpartsdiscount.com and save time and money on all your upcoming repairs. Given all the hassles associated with repairing damage to your car or truck, let www.carpartsdiscount.com be the number one store you go to make buying it easy. If your car or truck was built in 1995, 1994, 1993, 1992, 1991, 1990, 1989, 1988, 1987, 1986, 1985, 1984, 1983, 1982, 1981, 1980, 1979, then you will find the correct GMC G1500 Drive & Serpentine Belt parts right here.


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
JotForm Support
Hello kingsart,
Yes it is possible to achieve your requirement. You may like to add {edit_link} tag in the auto-responder email alert of your form. When the user submits the form, they will receive an auto-responder email which will have a clickable link. Clicking on this link will allow the used to edit their own submission data. The following guide should help you: http://www.jotform.com/help/40-How-to-Let-Users-Update-Their-Form-Submissions-at-a-Later-Date
Hope this helps.
Do get back to us if you have any questions.
Thank you!


text:
Houston Service Workers Demand Livable Wages
Service workers go on strike at a Burger King near the Galleria.
Meagan Flynn
Each day, Rasheedah McClenon drives over to her client's home to keep her company. Her client just had a heart attack, and McClenon, who works for a home health care agency, is there to remind her to take her medicine, to run errands for her, to clean her home and to make sure she's okay. "I'm a family member and a friend to her," McClenon said.
But she also pointed out the irony of her job: She's hired to take care of other families' homes — but isn't paid enough to take care of her own. The single mom with two boys makes $8.50 an hour.
"We take care of people's lives," she said. "We do important work, and we love our work. We just want to make a living wage for it."
That's why McClenon started protesting with Fighting For $15, a grassroots group asking lawmakers to raise the minimum wage to $15 an hour. On Thursday, just ahead of the Republican presidential debate at the University of Houston, about 40 protesters walked out on their fast-food jobs and service jobs and shouted for livable wages at a Burger King near the Galleria — many were single parents and immigrants. And they planned to take their signs to UH so the Republican candidates would hear their demands.
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In many cities across America, those demands have in fact been heard. The group is on the ground in 190 cities across America and has seen the minimum wage rise to $15 in places like San Francisco, Seattle, New York and Los Angeles. In Texas, there are about 5.4 million people paid less than $15 an hour, the second largest number in the nation according to data from Fighting For $15. In Houston — and the state as a whole — the minimum wage is $7.25.
"There's a lot that I could do with that raise," said Tanzie Dorough, a single mom who works at Burger King. "I'm fighting for it because I'm trying to better my children's future. I don't want them going through the same thing I'm going through.
She's been protesting for two years, the same length of time she's been working in fast food. She makes $200 every two weeks at $7.75 an hour, six nights a week every week. Since she works the closing shift, she has to hire a babysitter to watch her three kids, too, and said she barely gets any time with them. Her rent is $900 a month. She receives no benefits or health insurance, should her kids get sick or hurt.
"I feel like I'm a workaholic, which I'm not getting paid for. I don't get no overtime. It's hard," she said. "I feel like it's not right for them to work us like slaves."
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WEST VANCOUVER, B.C. — Shaun White and Conan O'Brien were talking, just one tomato to another, when the TV host offered some advice.
Click here for a slide show of Shaun White
Dude, like, lose the "Flying Tomato" nickname already. It's so 2006.
So White, an overwhelming favorite to defend his Olympic snowboard halfpipe title on Cypress Mountain on Wednesday night, is playing the name game these days, in search of not only a new nickname for himself but a handle for this latest gravity defying trick.
So far "The Animal" is the early leader in clubhouse.
"Apparently I resemble the drummer from the Muppets," White said with a shrug, his unruly mass of red hair escaping out from underneath a cap. "So I'm 'The Animal.' I guess I'm Shaun 'the Animal' White."
Or how about Red Zeppelin, as a teammate recently suggested?
After all, isn't White, the 23-year-old from Carlsbad, the ultimate Olympic rock star?
"He's not just in the snowboarding community big," said U.S. snowboarder Louis Vito. "He's mainstream big."
White is at least an $8 million a year industry with his own video game, with not one but two clothing lines, a line of eyewear and his own top secret mountain halfpipe site, which he was delivered to by helicopter. A pop icon whose mainstream appeal has been confirmed by endorsement deals with American Express and Target. A guitar pounding, 1260-degree spinning megastar with a double twist who celebrated his 2006 Olympic victory on the cover of, what else, Rolling Stone.
More Olympics news: Blog | Photo galleries | TV schedule | All coverage
"I call him the Mike Jordan of boarding," said U.S. teammate Scotty Lago. "He's the best of the best."
"Shaun transcends the sport," said Ross Rebagliati, the first Olympic snowboard champion. "He's giant."
The question is, is White too big? Is he now too corporate to be cool? Is he too good for his and the sport's own good?
White has pushed the edge of the envelope so far with his latest trick, called for now, the double McTwist 1260, in which he does two head over heels midair flips over the 22-foot halfpipe while spinning through 31/2 revolutions, that many in and out of the sport fear he is flying into dangerous air space.
"Being the guy that's had a big hand in inventing these tricks, I'd say it's outrageous," White said, his voice rising in mock indignation. "It's tough to say. Obviously, there have been injuries that have happened to people who are close to us. We're under a magnifying glass. That's just a part of what we do. We fall. We get back up and try it again. That's the best part of our sport. You can take a crash, come back and succeed over it, and that's the best feeling you can have."
Safety concerns about the sport moved into the Olympic spotlight after White crashed on the top side of the halfpipe at the recent X Games in Aspen, leaving a strawberry size scrape on his left cheek.
"I'd be lying to say that crash didn't shake me up," White said. "I was all over the place. I got to the bottom and it dawned on me. I thought I had my helmet on, and it was gone.
"I think our sport was pretty dangerous to begin with. We drive around in cars and that's pretty dangerous, and we still do it."
The kid whose family slept in a van or cheap motels while he competed in early snowboard competitions, now has a collection of exotic cars that included a Lamborghini until he crashed it.
The fancy wheels are just another reminder of how far White has elevated himself above his rebel sport. Billed on the cover of Rolling Stone four years ago as the "Coolest Kid In America," White is now a player on Madison Avenue, Corporate America's — and the Olympic movement's — ticket into a $150 billion a year youth market.
He has a street wear clothing line with Target, a winter clothing collection with Burton, which according to its catalog claims to have everything a "White Knight" needs including the "Most Unholy Jacket Ever" and mid weight pants that have a "four-way stretch for added tweakability and stink-proofing for odor-free autograph sessions."
White is laughing all the way to the bank. Some in the sport suggest a recent Forbes report that estimated his 2009 income at $8 million was low. His video game has sold more than three million copies. In addition to the Target, Burton and American Express deals, he also has endorsement contracts with Hewlett Packard, Oakley and Red Bull.
The latter spent $1 million building White his own private halfpipe with a foam pit on a remote Colorado mountainside. So remote that White had to be helicoptered in. It was during the top secret Project X that White developed the McTwist.
The project raised charges of elitism in a sport that still jealousy guards its street cred.
"If there this anything, it's jealousy," Vito said. "I mean, I would have loved to have a sponsor throw down over a half-million dollars to build me a private pipe with the best pipe builder and a foam pit. You know, it is what it is. And that's cool for him."
White dismisses the criticism, insisting at heart, he is still just a skateboard punk from Carlsbad.
"I'm pretty sure I would have been corrupted a long, long time ago," he said "If I was going to do it, if I was going to go down that path and turn into some other guy, I definitely think it would have happened already. I'm glad that money hasn't changed me and I'm glad that my family is still the same people. I go home and I have to, you know, clean up after the dog and the whole deal.
"Obviously, (his notoriety has) changed dramatically from when I first started this sport. Being 6 years old starting this sport and having the whole family travel in a van. All five of us would be in Motel 6, making food, setting off the fire alarms, the whole deal. That's where I came from in the sport, and that's something I wouldn't trade anything in the world for. That's where I came from and what I know. Know what I mean? Having been there and now making a great living in the sport, and being the one to beat, I don't know, it feels great. It makes it that much more special. I really feel like I earned it and I really feel like I can be a voice in the sport."
Now if he could only find a permanent name for the double 1260.
"We've had a couple good names, the Tomahawk, that was the steak I ate while I was in Aspen," White said. "It was massive. Either that or the Double Eagle. That sounds pretty delicious."
Contact the writer: email@example.com


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'Preach' marks the official arrival of a different kind of girl group. Despite working their way up to this moment for the last 18 months, this song is the first true introduction to M.O. and what we can expect from them in 2015. With a passion for experimentation and a real authenticity that most traditional girl groups would kill for, this trio aren't afraid to be who they are. In their quest to develop their own brand of real, throw-back R&B they have come-up with something standout: Their impossibly catchy new single, 'Preach' is due to be released on May 3rd.
The girls evoke a classic period for R&B trios, the era of TLC, SWV, En Vogue - the best of the nineties' feisty and assertive characters that combined gloss, pure groove and the ability to dance with a polished production and sassy vocal. Initially causing a stir when they teamed up with Lady Leshurr for a version of 1998 Brandy & Monica hit, 'The Boy Is Mine', M.O. signalled the emergence of a new kind of pop-R&B and quite possibly the birth of the first credible UK R&B group in a generation.
Described by NME as "The British TLC", Annie, Frankee and Nadine have been quietly and steadily doing things the right way: staying true to themselves, working hard on new material and collaborating with exciting and contemporary UK producers (Woz, Bless Beats, Gorgon City and recently featuring on hipster-as-hell producer Linden Jay's remix of Destiny's Child's "Bug A Boo'), as well as certifiably big US producers James Fauntleroy (Drake, J.Cole, Travis Scott) and Rahki (Kendrick Lemar, Eminem, Aloe Blacc).
Hailing from Sheffield, Manchester and Aylesbury respectively, M.O. have been writing, recording and performing together for the last two years; sharing the stage with some big names including; Jess Glynne, Ciara, Rudimental and Disclosure, things look set to get even bigger for the group this year when they perform at The Great Escape and support rising MC, Stormzy in April.
Having already been championed across the specialist spectrum (Annie Mac, MistaJam), it's clear that M.O. are ready to snatch up the crown that the critics (including; Vogue, Hunger, Dazed and i-D) rightly say is theirs for the taking. M.O might just be the outspoken renaissance women that we have been waiting for and even better, they're ours!


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Nice rooms
Staff was very friendly and helpful
Friendly environment - highly recommend to young travelers looking to meet new people
Bathrooms were clean
Location is in old town


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Now, on to some fun painting scoop!
This Anthony dresser and matching side tables were so much fun to paint. I used a base coat of Annie Sloan's Old White and CeCe Caldwell's Smokey Mountain Grey on top. For the tall dresser, I decided to leave some of the original wood peeking through and painted the doors with Old White.
Sadly, my iPhone pictures don't do these pieces justice, and they actually sold very quickly at the store so I won't get any second chances.
The side tables have a very monochromatic look and feel with some distinct distressing to show off the Old White underneath.
Below is an unclose of the carved wood and knob. The wood underneath and the Old White actually blended with the Annie Sloan dark wax to provide an almost golden hue.
All in all, the project was a lot of fun, and I was very excited with the three toned dresser and monochromatic side tables.
Thanks for coming along for the ride. It's going to be a short one today because I have a custom paint job that I need to finish up.
Linking Up on Monday:


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Question: The mean number of crime reports filed per hour by
The mean number of crime reports filed per hour by the Millennium City Police Department is 0.833. What is the probability that in a 24-hour period 10 or fewer crime reports will be filed by the Millennium City Police Department? What is the probability that the Department will file 20 or more crime reports in a 24-hour period?
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I don't want to read this. You don't want to read this. But for the past couple of years it's like we're stuck on the set of "War of the Roses" between Dwyane Wade and his wife, Siovaughn. It's not even entertaining in some awful, voyeuristic sense. It's just sad.
The latest, craziest chapter is Siovaughn filing a lawsuit against Wade's apparent girlfriend, Gabrielle Union. It alleges Union "engaged in sexual foreplay" in front of the two sons and that (seriously) Wade gave a bigger Christmas present to Union than to the sons.
Can this just please end?
It started in 2007 with their separation and Siovaughn later claiming she got a sexually-transmitted disease from him. That's been refuted by the deposition of a close friend of Siovaughn's saying she got the STD from another man.
Siovaughn has criticized Wade as a father. He has responded by saying she participates in "alternative avenues of worship that are completely outside of the status quo" and that his children should not be raised in that environment.
We've had Wade ask a court to give him full custody of his two sons while claiming his wife is, "unstable, dishonest, unbalanced, unwilling to accept responsibility for her actions, not of good character, and therefore, unfit to be a custodial parent."
We've had Siovaughn going on E! talking of the problems - "I stopped fussing at him about staying out late at night and I started staying out late at night."
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- downcourt
- adverb or adjective Date: 1952 in or into the opposite end of the court (as in basketball)
New Collegiate Dictionary. 2001.
New Collegiate Dictionary. 2001.
downcourt — ☆ downcourt [doun′kôrt′] adv., adj. Basketball into, toward, or in the opposite half of the court [dribbling downcourt] … English World dictionary
downcourt — /down kawrt , kohrt /, Basketball. adv. 1. to or into the opposite end of the court. adj. 2. directed to or into the opposite end of the court. [1950 55; DOWN1 + COURT] * * * … Universalium
downcourt — adverb Toward ones teams offensive basket Ant: upcourt … Wiktionary
downcourt — ˈ ̷ ̷| ̷ ̷ adverb (or adjective) : in or into the direction of the goal (as in basketball) … Useful english dictionary
Cherry picking (basketball) — For the fallacy see: Cherry picking (fallacy) Cherry picking is a strategic act sometimes used in basketball to give a team a higher scoring chance.[1] A player who performs this act is known as a cherry picker. It is sometimes frowned upon, and… … Wikipedia
down|court — «DOWN KRT, KOHRT», adjective, adverb. Basketball. in or toward the area of the offensive basket: »a downcourt pass (adj.). He dribbled downcourt and scored (adv.) … Useful english dictionary
down·court — /ˈdaʊnˈkoɚt/ adv : into or toward the opposite end of a basketball court He passed the ball downcourt. running downcourt … Useful english dictionary
Washington Wizards — Washington Bullets redirects here. For the unrelated song by The Clash, see Washington Bullets (song). Washington Wizards 2011–12 Washington Wizards season … Wikipedia
Red Auerbach — Infobox Celebrity name = Arnold Jacob Red Auerbach caption = Auerbach speaking after being honored with the Lone Sailor Award on October 25, 2006 birth date = birth date|mf=yes|1917|9|20|mf=y birth place = Brooklyn, New York, U.S. death date =… … Wikipedia
Eric Anderson (basketball) — Eric Walfred Anderson (born May 26 1970 in Chicago, Illinois) is a retired American college and professional basketball player. [Not the same [http://www.basketballreference.com/draft/draftyear.htm?lg=n yr=1975 Eric Anderson] who was selected by… … Wikipedia