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Skip to comments.Lessons From A Capitalist Thanksgiving
Posted on 11/02/2013 1:11:25 AM PDT by Cincinatus' Wife
Its astonishing and a little horrifying that Americas elites know so little about their countrys history. Case in point: Jared Bernstein of the Economic Policy Institute. Jared is an influential left-ish economic polemicist and a sometime adviser to Barack Obama on economic affairs.....
On Monday night, I told Larry Kudlow about the story of the first Thanksgiving.
I explained that the first Thanksgiving was a celebration of abundance after a period of socialism and starvation. It seems Bernstein never heard about this chapter in U.S. history; he called it an exercise in revisionist history. Admitting that he had never read the memoirs of Plymouth governor William Bradford, he nevertheless dismissed the story as untrue. But the facts are undeniable, and there is nothing to revise. Bradfords historical accounts, which I quote below, have been read by schoolchildren for over 300 years.
[BIG SNIP--Be sure to read]
Its genuinely scary to me that a leading member of the left intellectual establishmenta group that will shortly rule both of our elected branchesdoesnt know about Americas first experiment with socialism. On top of that, he doesnt care to know. Neither did the philosopher kings who,ignorantly and blithely, imposed on our forebears a system that led to malnutrition, pestilence and mass fatalities.
But it has always been that way. Men in ivory towers, ivied halls,foundation-funded think tanks or bustling newsrooms dream up new forms of social organization. They write books,policy papers and five-year plans telling us all that is wrong with the way we live now and what could be done if we simply adhere to their analyses.
...........When we remind them that their ideas have been triedand found wantingin the past, they cavalierly deny history, clap their hands over their ears and cry even more loudly for change. If we listen to them, we deserve what we will get.
(Excerpt) Read more at forbes.com ...
Occupy Plymouth Colony: How A Failed Commune Led To Thanksgiving [excerpt] ".......................As my friend Reuven Brenner has taught me, history is a series of experiments: The Human Gamble. Some gambles work and are adopted by history and some do not and should be abandoned by it. The problem is that the human gamble only works if there is a record of experimental outcomes and if decision makers consult that record. For many years, the story of the first failed commune of Plymouth Bay was part of the collective memory of American students. But Progressive Education found that story unhelpful and it has fallen into obscurity, which explains why (as I alluded to before) a well-educated establishment figure like Jared Bernstein would be unaware of it.
Im often asked why our current leadership class forgets the lessons of the past so often. They are, after all, very smart men and women. Dont they know that collectivism will fail?
No, they dont. Not anymore. For much of our history, our leaders were educated in the principles which were to help them avoid errors once theyd joined the ruling class. They studied to learn how to not misuse power. Now our leaders learn nothing of the dangers of abusing power: their education is entirely geared to its acquisition.
All of their neurons are trained on that one objective to get to the top. What they do when they get there is a matter for later. And what happens to the country when theyre done with their experiments is beside the point: after all, their experiments will not really affect them personally. History is the story of the limitations of human power. But the limits of power is a topic for people who doubt themselves and their right to rule, not the self-anointed............................"
David Horowitz [written during the Bill/Hillary Clinton presidency: Progressive Narcissism "..........Conservatives who think progressives are misinformed idealists will always be blind-sided by the sheer malice of the left -- by the cynicism of those who pride themselves on their principles; by the viciousness of those who champion sensitivity; by the intolerance of those who call themselves liberal; and by the ruthless disregard for the well-being of the poor on the part of those who preen themselves as their champions.
Conservatives are surprised because they see progressives as merely misguided, when they are, in fact, morally and ontologically -- misdirected. They are the messianists of a false religious faith. Since the redeemed future that justifies their existence and rationalizes their hypocrisy can never be realized, what really motivates progressives is a modern idolatry: their limitless passion for the continuance of Them."
Now these aides will have to wait a year to learn whether the source of their medical plans matters a whit to the voters.
Given the abysmal approval ratings that members of Congress are confronting, and the welter of public apprehension about the health law thats making Republicans giddy and Democrats anxious, its tough to imagine any close race in 2014 being influenced if the incumbents staff is still covered under the federal health system, not one of the Affordable Care Acts exchanges.
But since the suggestion of hypocrisy is the mother of so many 30-second attack ads, its not unreasonable to imagine some challengers at least trying to make a big deal out of lawmakers who have decided their employees wont have to enter the new marketplaces into which they have recently steered millions of constituents. Fairly or not, these critics will describe this as fresh evidence of Congress going out of its way to exempt itself from the social experiments it imposes on the rest of the nation..................." Hill Staff Health Care: Will Voters Care About This Sideshow?
the real shame of it IS-----> they got away with it..
Capitalists were too busy to make SOCIALISM ILLEGAL..
Here is another good one below. It brings up the idea that it wasn't discontent among the colonists, but rather the fact that they had to send large amounts of their labor back to the capitalist investors back home. No doubt the "progressives" will try to use this to refute the reasons for the failure of socialism. The author shows that while this is partly true, the main problem was that the workers resented the slackers. READ THIS ONE!
I just got mine but the horse is smarter than obama's entire cabinet.
Needs to be repeated. To a Christian, this is obvious.
I agree!
The Gods of the Copybook Headings, Kipling
As I pass through my incarnations in every age and race,
I Make my proper prostrations to the Gods of the Market-Place.
Peering through reverent fingers I watch them flourish and fall,
And the Gods of the Copybook Headings, I notice, outlast them all.
We were living in trees when they met us. They showed us each in turn
That Water would certainly wet us, as Fire would certainly burn:
But we found them lacking in Uplift, Vision and Breadth of Mind,
So we left them to teach the Gorillas while we followed the March of Mankind.
We moved as the Spirit listed. They never altered their pace,
Being neither cloud nor wind-borne like the Gods of the Market-Place.
But they always caught up with our progress, and presently word would come
That a tribe had been wiped off its icefield, or the lights had gone out in Rome.
With the Hopes that our World is built on they were utterly out of touch
They denied that the Moon was Stilton; they denied she was even Dutch
They denied that Wishes were Horses; they denied that a Pig had Wings.
So we worshipped the Gods of the Market Who promised these beautiful things.
When the Cambrian measures were forming, They promised perpetual peace.
They swore, if we gave them our weapons, that the wars of the tribes would cease.
But when we disarmed They sold us and delivered us bound to our foe,
And the Gods of the Copybook Heading said: "Stick to the Devil you know."
On the first Feminian Sandstones we were promised the Fuller Life
(Which started by loving our neighbour and ended by loving his wife)
Till our women had no more children and the men lost reason and faith,
And the Gods of the Copybook Headings said: "The Wages of Sin is Death."
In the Carboniferous Epoch we were promised abundance for all,
By robbing selected Peter to pay for collective Paul;
But, though we had plenty of money, there was nothing our money could buy,
And the Gods of the Copybook Headings said: "If you don't work you die."
Then the Gods of the Market tumbled, and their smooth-tongued wizards withdrew,
And the hearts of the meanest were humbled and began to believe it was true
That All is not Gold that Glitters, and Two and Two make Four —
And the Gods of the Copybook Headings limped up to explain it once more.
* * * * *
As it will be in the future, it was at the birth of Man —
There are only four things certain since Social Progress began —
That the Dog returns to his Vomit and the Sow returns to her Mire,
And the burnt Fool's bandaged finger goes wabbling back to the Fire —
And that after this is accomplished, and the brave new world begins
When all men are paid for existing and no man must pay for his sins
As surely as Water will wet us, as surely as Fire will burn
The Gods of the Copybook Headings with terror and slaughter return!
"When all men are paid for existing and no man must pay for his sins"
He nailed it on this and many other areas.
Of course, he is a white racist colonialist and should not be taught anymore./s
Yes, Kipling's prescient poetry portends both hope and dread.
I have heard Rush mention this before. The problem is that this is only part of the true story.
The first year everyone worked for the common good and a large harvest was realized. But with additional people arriving that fall there was not enough food for all. The second year, the leaders decided the provide motivation by saying individuals could keep what they grew. There was a drought for most of the growing season until the group realized their sin of greed. After they repent to God, a gentle rain came unlike the typical last season down pours. The harvest turned out large. The Pilgrims acknowledge the forgiveness of God and their own sin of greed.
It is not a story of the benefits of capitalism, but is the story of mans sinfulness and God's forgiveness.
Our rural, union teachers in a small Texas town never taught the true version of our First Thanksgiving. Thru the years, not one teacher referred to the true version. The only way I found the truth was listening to Rush, and David Barton. Just ONE reason Mulder had it right when he stated 'Trust No One'
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Hoover Anniversary WindTunnel Canister Vacuum S3670 Reviews
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Read 9 Reviews
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Comments about Hoover Anniversary WindTunnel Canister Vacuum:
You would think an anniversary "anything" would be something special. This isn't. Originally I was looking for a vacuum with two prerequisites: a good HEPA system and venting out the top of the unit. This canister offered both. I never liked vacuums that vent out the back, blowing particulate all over the house – it doesn't make sense. The beater brush for furniture and carpets is powerful and well made.
Hauling around this canister is no easy task with the tine wheels that can't even roll over its own cord. POOR DESIGN.
I feel the handle on every canister should have an on/off button. This model doesn't. Again, this doesn't make sense. The other thing that drives me crazy is the connection of the floor brush. It's a simple friction fit which is difficult for me, a fairly strong male, to pull off. There's no button to depress, making it easier to remove. Anyone with muscle weakness will become quickly frustrated with this obvious design flaw. The crevice tool is much too short.
The suction tube is WAY too thin and got out of round after the first fall to the floor. The cord's rewind system is cheap. After one week t started rewinding on its own. I called the company which wanted it returned to fix it. Weeks without a vacuum – no way. They should have an agreement with local stores to fix these problems.
Comments about Hoover Anniversary WindTunnel Canister Vacuum:
The warranty period & terms will not begin to cover what will go wrong with this product. 2 years and nothing but agrravation. I would strongly recommend you purchase another vaccum. Hoover used to be the best...now it is much less that that.
Comments about Hoover Anniversary WindTunnel Canister Vacuum:
This vacumn is great for someone who needs to clean constantly. most vacumns can get the job done, but for someone that has kids and pets, more than stnadard cleaning is needed. this vacumn is great for someone that knows they will use it at least once a week, and needs it to last more than a while. it gets pet hairs, toddler dirt from shoes, food, etc. It's great for a family of more than a few, and pets too. 4/5 stars at least.
Comments about Hoover Anniversary WindTunnel Canister Vacuum:
The **Hoover S3670 Bagged Canister Vacuum Vacuum is a great product. I really liked the it's manuverability, and efficency. I did not like the way you clean it out though, and its kind of a pain clicking the bag compartment back itno place.**
Comments about Hoover Anniversary WindTunnel Canister Vacuum:
I definetly wasn't satified with this vacuum. My bag broke and we returned it and got a new one. It doesn't pick up that great to me. It was more than i wanted to pay but with the hoover name i thought i would get my moneys worth.
Comments about Hoover Anniversary WindTunnel Canister Vacuum:
The Hoover S3670 is a Dream for my Carpets. It revitalized and beautified all of my home'scarpets. It's unique style and lightweight characteristic makes me enjoy vacuuming.
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This new and exciting vacuum should become a part of every homeowner's appliances. See it know at most retailers.
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Comments about Hoover Anniversary WindTunnel Canister Vacuum:
The Hoover S3670 Bagged Canister Vacuum, is a powerful vacuum it gets everything, but its many moving parts make it difficult to maneuvers, but it also has the readability of a hoover and is practicallydust free. It is an amazingng vacuum, if you have the money I would invest in this vacuum. Some things that might ad caution is it uses bag instead of a emptable container, its not a push vacuums, those are the only two major turn off to it.
Comments about Hoover Anniversary WindTunnel Canister Vacuum:
I have had a Hoover wind tunnel for the past month, I also have a bagless vacuum. I have been using both to vacuum my floors so i can see with is better. Hoover has made consistently good vacuums. I believe that the air in my home is cleaner smelling after a round of vacuuming with my Hoover. This vacuum dose work much better then most bagless vacuums. This vacuum has WindTunnel technology just like the bagless kind. It also has Powered hand tools,Full bag indicator, a hepa filter,a 7' foot hose and a automatic cord rewind with 25' foot cord. smost vacuums don't have this featcher. Great suction, very durable, easy to maneuver and very high quality! The design of the unit is very nice, it is light enough to carry up to our second floor and do the stairs in between. The attachments work well and I would reccomend this vacuum cleaner to anyone looking for a canister style cleaner. I am extremely pleaed with this vacuum, I give it a five star ratting. I would recommend purchasing a hoover if you like the bagged kind of vacuum.
Comments about Hoover Anniversary WindTunnel Canister Vacuum:
I have owned a Kirby vacuum for as long as I can remember, and my daughter has been telling me that I need a new vacuum for a long time. But for some reason, I just couldn't let go of my Kirby. It wasn't until my friend showed me her new Hoover S3670 vacuum that I decided to get rid of my Kirby.

subset: commoncrawl


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Primary cash balance posts deficit of 46 mln at end of July
Industrial turnover up by 8.1 pct in June for eighth consecutive rise
Budget primary surplus at 3.05 bln in 7-month, above target on underspend
Building activity up by 45.7 pct in May, highest since Dec 2005
Eurosystem funding drops by 3.9 bln in July on big decline in ELA
Unemployment at 21.7 pct in May after revised April figure
Loan and deposit rates edge down in May but remain above euro area average
Following a drop by 10 basis points (bps) in April, the interest rate on households' new time deposits fell further by 18 bps 2.53 percent in May, according to the Bank of Greece (BoG).
The year to date reduction stands at 30 bps, while the current rate is half the record high of 5 percent posted in June 2012.
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The King Drapes uncompromising, guitar-driven sound puts them at the heavy end of Rockabilly, and singer Anu-Katja's vocal captures the attention not only on the uptempo songs, but also on the slower numbers where she is particularly melodic.The King Drapes released their first album King Drapes Stomp on Raucous in 1993, and since then have successfully toured the UK, mainland Europe and Scandinavia where they also made TV appearances.Rockabilly Rules features 15 diverse tracks.The originals are outstanding, often trashing the boundaries of Rockabilly purism, returning to cover Revival Rock 'n' Roll classics Gunning For The Dog and Rockabilly Rules OK.
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Six Pixels of Separation - The Mirum Blog is marketing and communications insights from the edge. Mitch Joel will unravel the complex world of digital marketing and social media with the perspective of a digital marketing agency. The Six Pixels Of Separation podcast is also here. Join the digital marketing conversation. Take part in the new marketing discussion. Stay tuned to see how this interactive agency is making a digital difference. While marketers still struggle to understand online marketing, an entirely new generation of marketing opportunities is already taking place and they're being created by your customers. The Twist Image blog is here for all of your digital marketing adventures.
June 25th, 2006
Episode 6 of 577 episodes
Don't let the running time of this episode scare you. I've set it up so you can enjoy Six Pixels Of Separation in its entirety, and if you care for the last segment, you can stick around for the extra fifteen minutes. Between interviewing Ken Schafer of One Degree and the amazing experience I had with Across The Sound/Jaffe Juice/Author of the Life After The 30-Second Spot, Joseph Jaffe, and For Immediate Release Co-Host, Neville Hobson at the Second Life Avatar-Based Marketing conference, this is a fast-paced edition. Here it is: Six Pixels Of Separation - Episode #6 - Host: Mitch Joel. - Running time: 43:06. - Audio comment line is active - 206-666-3772 (I will only play questions or comments that push the conversation forward). - Comments and Trackback are now live on the Twist Image Blog. - Audio comment and question from Sulemaan Ahmed - Online Channel Manager - Sears Travel regarding RSS Versus Email. - Upcoming events: - Blogs - A Marketers' Secret Weapon - CMA - Canadian Marketing Association. - CaseCamp Montreal. - Still looking for someone to present a case study in French. Sign-up here: CaseCamp Montreal. - CaseCamp Montreal banners at One Degree (thanks Ken). - Digital Marketing Conference - CMA - Canadian Marketing Association. - Hold the date: October 19th and 20th in Toronto. - Announcement of keynote speakers: Joseph Jaffe, Bryan Eisenberg, C.C. Chapman, David Weinberger and yours truly. - Free copy of Bryan Eisenberg's book, Waiting For Your Cat To Bark? for all registered participants. - Great ad for the 2006 Digital Marketing Conference in Marketing Magazine. - Interview with One Degree creator Ken Schafer. - Avatar-Based Marketing Conference in Second Life. - Skype call between Mitch Till (me), Divo Dapto (Joseph Jaffe) and Jangles Junot (Neville Hobson). As always, your comments, feedback and help on Six Pixels Of Separation is appreciated. Please let me know what you think. Download the Podcast here: Six Pixels Of Separation - Episode #6 - Host: Mitch Joel.
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PTZ Camera With Direct To Fibre Link
: 17 December, 2010
(New Product)
Siqura's new range of IP PTZ cameras can be connected directly to fibre optic networks for simplified long-distance external camera installations
Optelecom-NKF has announced the release of its Siqura IP PTZ camera line with a flexible SFP interface for directly connecting to a fibre optic network. This feature simplifies both the setup and maintenance of roadside camera installations where a fibre network is utilized to overcome long distance limitations.
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Single-cell measurements allow a unique glimpse into cell-to-cell heterogeneity; even small changes in selected cells can have a profound impact on an organism's physiology. Here an integrated approach to single-cell chemical sampling and assay are described. Capillary electrophoresis (CE) with laser-induced native fluorescence (LINF) has the sensitivity to characterize natively-fluorescent indoles and catechols within individual cells. While the separation and detection approaches are well established, the sampling and injection of individually selected cells requires new approaches. We describe an optimized system that interfaces a single-beam optical trap with CE and multichannel LINF detection. A cell is localized within the trap and then the capillary inlet is positioned near the cell using a computer-controlled micromanipulator. Hydrodynamic injection allows cell lysis to occur within the capillary inlet, followed by the CE separation and LINF detection. The use of multiple emission wavelengths allows improved analyte identification based on differences in analyte fluorescence emission profiles and migration time. The system enables injections of individual rat pinealocytes and quantification of their endogenous indoles, including serotonin, N-acetyl-serotonin, 5-hydroxyindole-3-acetic acid, tryptophol and others. The amounts detected in individual cells incubated in 5-hydroxytryptophan ranged from 10−14 mol to 10−16 mol, an order of magnitude higher than observed in untreated pinealocytes.
Single-cell analysis is becoming more important as even so-called "homogenous" cell populations have shown significant cell-to-cell heterogeneity.1,2 However, measuring the contents of individual cells presents several challenges. Cells contain a broad range of compounds that span many orders of magnitude. When working with a tissue homogenate, much of the information on individual cell composition is lost. Addressing these concerns requires technologies that enable single-cell sampling and analysis. Capillary electrophoresis (CE) with laser-induced fluorescence detection (LIF) is particularly appropriate for single-cell chemical analysis due to its ability to probe both mass- and volume-limited samples, produce high separation efficiencies, and detect and quantitate low-abundance analytes.3–5 While CE-LIF has the sensitivity to characterize trace analytes within individual cells and subcellular components, and is a well-established approach, robust single-cell sample preparation methodologies are still evolving. As highlighted in this issue6 options include manual7–9 and automated mechanical10,11 cell handling, microfluidics,12,13 laser-assisted cell lysis,14,15 and, the focus of this article, optical trapping.16–18
Optical trapping was first demonstrated by Ashkin in 1970;19 in 1987 he extended the method to examining single bacteria, viruses, and cells.20,21 Subsequently, its use for studying biological structures has grown dramatically and trapping has become an invaluable technique for studying micrometer- and nanometer-sized objects not only in biology, but in physics and chemistry as well.22,23 Optical trapping is considered a non-contact method as it minimizes outside mechanical interferences that may affect the analytical measurements. There are also a variety of optical trap (OT) types and possible modifications that can be used to optimize the technique for a particular application and sample type.24–26 OT designs allow their hyphenation with other technologies, such as microfluidics,27–29 CE,30,31 Raman32,33 and fluorescence spectroscopy.34,35 Overall, OTs are a versatile tool that can be used to study, isolate, and manipulate single cells and subcellular organelles on the micron scale with a minimum of mechanical interference.
The successful development and optimization of a bioanalytical approach depends on choosing the appropriate biological model. The size and shape of the cells of interest, their biochemical content, and the surrounding extracellular media can add complexity to optical trapping experiments. Located in the pineal gland, pinealocytes are an attractive model for developing an approach that utilizes hyphenated optical trapping and CE laser induced native fluorescence (LINF). The cells of the mammalian pineal gland contain several natively fluorescent indolamines, including serotonin and melatonin, in high concentrations compared to other parts of the central nervous system.36,37 A critical feature of the pineal gland is the large fluctuations in chemical content depending on the time of day. During the day, serotonin is present at a relatively constant basal level whereas N-acetylserotonin (NAS) (a metabolite of serotonin and the precursor to melatonin) and melatonin are present at low levels, if at all. Once the retina signals that darkness has fallen, serotonin synthesis increases by several fold; within an hour, NAS and melatonin levels increase dramatically as they are synthesized, and melatonin is secreted into the blood. Within 1–3 h of daylight, NAS and melatonin production ceases. A number of other indolamines have been reported in the pineal gland, including 5-methoxytryptophan and 5-methoxytryptamine.38,39
In this work we describe an OT multichannel (MC) CE-LINF system optimized for the native fluorescence detection of catecholamines and indolamines.40–42 Analytes are identified by the combination of their unique spectral characteristics and migration times. The OT-MC-CE-LINF instrument enables analysis of a single pinealocyte with a minimum of sample handling and disruption, provides a separation step to reduce chemical complexity, and separates similar analytes and potentially concentrates them. Once the cell is localized within the OT, the capillary inlet is moved adjacent to the trap using a computer-controlled micromanipulator and microscope combination. The cell is released from the OT and quickly injected into the capillary, where it is chemically lysed and its chemical components are separated and detected. The ability of the OT-MC-CE-LINF system to inject individual cells and separate and characterize their indolamines is demonstrated.
Chemicals, unless otherwise noted, were purchased from Sigma Aldrich (St. Louis, MO) at reagent grade or higher. Citric acid sheath buffer (25 mM, pH 2.25) was made by dissolving 5.25 g of C6H8O7•H2O in 1 L of ultrapure deionized (DI) water (Elga Purelab Ultra, Siemens Water Technologies, Warrendale, PA). Electrophoresis buffers were made by diluting a stock solution of 50 mM borate buffer, pH 8.8, prepared by dissolving 9.2 g of Na2B4O7•10 H2O and 3.0 g of B(OH)3 in 1 L of ultrapure DI water. For surfactant-containing electrophoresis buffers, 0.54 g of sodium dodecyl sulfate (SDS) was added to 50 mL of diluted borate buffer, pH 8.8, sonicated for 2 min to dissolve, and filtered with a 0.22 μm syringe filter (Nalgene, Rochester, NY). Serotonin (5-HT) (Alfa Aesar, Ward Hill, MA) and tyrosine (Tyr) were dissolved in 2.5 mM citric acid, pH 2.5, and sonicated on ice for 30 min. Tryptophan (Trp), NAS, 5-hydroxyindole acetic acid (HIAA), melatonin (MT), 5-hydroxytryptophan (HTP), 5-methoxytrytophol (MTOL), 5-methoxytryptamine (MOT) (TCI America, Portland, OR), tryptophol (TOL) (Research Organics, Inc., Cleveland, OH), 5-methoxyindole acetic acid (MIAA) (Gold Biotechnology, St. Louis, MO), and 5-hydroxytryptophol (HTOL) (Gold Biotechnology) were dissolved in 2.5 mM citric acid, pH 2.5, + 10% v/v acetone and sonicated on ice for 30–60 min. Standard buffers were prepared by diluting the sheath buffer 1:10 with ultrapure DI water. Fluorescein was prepared in ultrapure DI water. Standard stock solutions were diluted in either 1 mM borate buffer, pH 8.8 (1:50 dilution of stock borate electrophoresis buffer), or in high Ca+2/high Mg+2 modified Gey's balanced salt solution (high salt mGBSS), pH 7.2, consisting of 3.0 mM CaCl2 (0.44 g), 4.9 mM KCl (0.37 g), 0.2 mM KH2PO4 (0.03 g), 22 mM MgCl2 (4.47 g), 0.6 mM MgSO4 (0.07 g), 138 mM NaCl (8.06 g), 27.7 mM NaHCO3 (2.33 g), 0.8 mM Na2HPO4 (0.11 g), 25 mM HEPES (5.95 g), and 10 mM glucose (1.80 g) dissolved in 1 L of ultrapure DI water. Biological samples were stored in either high salt mGBSS, as described above, or mGBSS, pH 7.2, consisting of 1.5 mM CaCl2 (0.22 g), 4.9 mM KCl (0.37 g), 0.2 mM KH2PO4 (0.03 g), 11 mM MgCl2 (2.24 g), 0.3 mM MgSO4 (0.04 g), 138 mM NaCl (8.06 g), 27.7 mM NaHCO3 (2.33 g), 0.8 mM Na2HPO4 (0.11 g), 25 mM HEPES (5.95 g), and 10 mM glucose (1.80 g) dissolved in 1 L of ultrapure DI water. Buffers were filtered by a 0.45 μm bottle-top filter system (Nalgene, Rochester, NY) and degassed under vacuum with stirring for 30–60 min. NaOH was prepared by dissolving one pellet (~0.002 g) in 0.025 L of ultrapure DI water.
All experimental procedures were conducted according to protocols approved by the Institutional Animal Care and Use Committee, University of Illinois at Urbana-Champaign. Animal care and experiments were performed in full compliance with the principles and procedures outlined in the National Institutes of Health Guide for the Care and Use of Laboratory Animals.
Long-Evans/BluGill rats (University of Illinois at Urbana-Champaign), which have been demonstrated to be genetically homogeneous by high density genome scan, were sacrificed by rapid decapitation and the pineal glands isolated from the central nervous system. Males, 6–8 weeks, were sacrificed during the day, between circadian time 3:00 – 5:00, and pineal dissection and preparation was completed within 30 min. Glands were manually triturated for pinealocyte isolation and stored in high salt mGBSS on ice until analysis, typically between 30–60 min.
Rat pinealocyte suspensions were split into two groups. The first was incubated at room temperature for 60 min in 200 μM HTP, dissolved in high salt mGBSS. The second control group had an equal volume of high salt mGBSS and was incubated at room temperature for 100 min before analysis.
The CE columns, 85–120 cm long, were made from 50 μm inner diameter, 360 μm outer diameter fused silica capillaries (Polymicro Technologies, Phoenix, AZ). The capillary inlets and outlets were etched with hydrofluoric acid (HF) in order to reduce the outer diameters and create sharply tapered tips with a 40° angle (see43 and Supplementary Information for details).
Unless otherwise noted, all custom-built components were designed and fabricated in-house. The optical axis is parallel to the optical table at a height of 20 cm until the beam is directed 90° vertically by a dichroic mirror. All of the optics are infrared (IR)-coated unless otherwise specified. The near infrared (NIR) beam is viewed with IR viewing cards (VC-1550 and F-IRC-HP, Thorlabs, Newton, New Jersey) and an IR viewer (IRV1-1700, Newport Corp., Irving, CA).
The OT design (Figure 1A) uses a 1064 nm diode-pumped solid state Nd:YAG laser (Compass 1064-2500MN, Coherent Inc., Santa Clara, CA) with a maximum output of 2.5 W. The laser operates in TEM00 mode and has a wavelength stability of < 1 cm−1. The beam has a nominal diameter of 0.4 mm, a divergence of < 3.5 mrad, a pointing stability of < ± 5%, and an ellipticity of < 1.1. It is air-cooled and turn-key operated. The beam is expanded by a 20× high energy beam expander (HB-20X, Newport Corp.) and directed by a pair of gold-coated mirrors (PF10-03-M01, Thorlabs) into a set of two plano-convex lenses (SPX029, Newport Corp.) in a 1:1 telescope configuration, used to steer and parfocalize the beam. The beam expander is housed in a precision gimbal optic mount (605-4, Newport Corp.) and translated in the x-, y-, and z-directions by a translation stage (UMR12.40, Newport Corp.) and heavy duty optical lab jack (L490, Thorlabs). Plano-convex lens 1 is mounted in a 3-axis optical mount (LP-1A-XYZ, Newport Corp.) located 1000 mm from the back aperture of the objective and plano-convex lens 2 is mounted in a 2-axis mount (LP-1A-XY, Newport Corp.) located 500 mm from the back aperture of the objective. This setup can be generalized as the distance between the back aperture of the objective and the steering optic is equal to 4f, where f is the focal length of the lens.44,45 In this case, the focal lengths of both of the plano-convex lenses are 250 mm. The beam is then directed into the epi-fluorescence port of the microscope (AxioObserver A1, Carl Zeiss, Jena, Germany) and directed into the back aperture of the objective (Objective C-Apochromat 63x/1.2 W Corr, 441777-9970-000, Carl Zeiss, Jena, Germany) by a dichroic mirror centered at 1064 nm (950dcsp-laser, Chroma Technology, Rockingham, VT).
Polystyrene bead stock solutions (1 μm (PS04N/5749) and 10 μm (PS06N/6955) in diameter, Bangs Laboratories, Fishers, IN), were diluted 10-fold to 100-fold in ultrapure DI water and then used to optimize the optical trap. The beads were contained on a coverslip (2735-246, Corning Inc.) by a vacuum grease ring (Silicon High Vacuum Grease, Dow Corning, Midland, MI). Laser power measurements were taken with a PM10 sensor and a LabMax-TOP meter (Coherent Inc.).
The injection port for the instrument is housed on a non-conductive breadboard platform on a microscope that is contained in a clear Plexiglas box. The capillary inlet has PEEK fittings (Upchurch Scientific, Oak Harbor, WA) and a FEP sleeve (Upchurch Scientific), attached to allow for fast and easy switching between the syringes and capillary holder.
The capillary is held in place in the instrument by a custom-built acetal resin (Delrin, E. I. duPont de Nemours & Co., Wilmington, DE) sheath flow cell. It enters at the top of the cell and is held in place by liquid-tight fittings (Upchurch Scientific). The sheath buffer enters the cuvette from the right side with respect to the optical table and exits from the bottom of the sheath flow cell. The quartz cuvette (Starna Cells, Atascadero, CA) used for excitation and detection of eluents is open on both ends and is attached to the top and bottom pieces of the sheath flow cell with Tra-Cast 3103 epoxy (Henkel Corp., Billerica, MA).
The instrument optics (Figure 1B) were based on our prior instrument design.46 Deep UV radiation (224.6 nm) from an HeAg hollow cathode ion laser (HeAg70, Photon Systems Inc., Covina, CA) is spectrally filtered using a four-bounce mirror configuration, attached to the front of the laser head. The beam is directed via two UV-coated mirrors (Thorlabs) into a laboratory-built lightproof, non-conductive box and breadboard, which houses the detection optics and protects against spurious arcing. The collimated beam is nominally focused using a plano-convex lens (OptoSigma, Santa Ana, CA) to a 50 μm spot directly below the outlet of the capillary, which has been HF-etched to a cone-shaped tip and is housed in a custom-built sheath flow cell, as described above. As analytes elute from the capillary they are excited by the focused beam and emit fluorescence, which is collected and collimated by a 15× all-reflective objective (13596, Newport Corp.). The fluorescence is directed toward three photomultiplier tube (PMT) detectors (H6780-06, Hamamatsu, Middlesex, NJ) by two dichroic mirrors (310dcxxr-haf #110258 and 400dcxru #111563, Chroma Technology), with transition points at 310 nm and 400 nm, respectively. The first detector (PMT "blue") measures emission from 250–310 nm, the second detector (PMT "green") measures emission from 310–400 nm, and the third detector (PMT "red") measures emission from 400 nm and above. The laser and PMTs are synchronized and controlled by software written in LABView and provided by Photon Systems Inc. Posts, post holders, and other optical mounts were purchased from Newport Corp., Melles Griot (Albuquerque, NM), or custom-built. Optical mounts for the focusing and collection optics are coated in Vinyl Liquid Electric Tape (Star Brite, Ft. Lauderdale, FL) and Scotch Super 88 electrical tape (3M, St. Paul, MN) to reduce arcing from the capillary outlet and tubing to the mounts.
Negative voltage for electrophoresis is applied to the sheath flow waste by a stainless steel cylinder connected to a power supply (PS/MJ30N0400-11, Glassman High Voltage, High Bridge, NJ) and laboratory-built control box. A 10 kΩ resistor and a digital multimeter (Fluke 76, Fluke Corp., Everett, WA) are part of the circuit and are used to measure the current across the capillary.
Sheath buffer is gravity-driven and flow can be adjusted by a right angle switching valve (Upchurch Scientific). High purity Teflon PFA Plus tubing and appropriate fittings were purchased from Upchurch Scientific. All tubing is further encased within FEP-lined polyethylene tubing (McMaster-Carr, Elmhurst, IL) to reduce static attraction and arcing during electrophoresis. Tubing between the optics box and the sheath box is also surrounded by four 16 oz. polyethylene containers and Scotch Super 88 electrical tape.
The optical trap and the MC-CE-LINF system are interfaced at the microscope stage (Figure 1S, Supplemental Information). The trap is located at the focal point of the objective, approximately 0.28 mm from the objective surface, including the coverslip thickness (0.13–0.16 mm). The capillary inlet is controlled by a computer-controlled motorized micromanipulator (MP-285, Sutter Instrument Co., Novato, CA), which has 1″ of travel in all three axes, two step sizes—coarse (0.2 μm/step) and fine (0.04 μm/step)—and a maximum speed of 2.9 mm/s. It has a tabletop controller and a rotary optical encoder for manual control; programmable robotic control is also available. The micromanipulator is mounted on a non-conductive optical breadboard, which is stabilized by two 1/4″-28-tapped beams that attach to the microscope stand on either side of the stage. The capillary is held in the micromanipulator by an acetal resin cylinder, which is 6″ long and has a 1/16″ diameter hole drilled in the center. This holder reduces the chance of arcing to the micromanipulator motors.
The sample is held on a coverslip holder, machined out of polycarbonate, with a lip to rest the coverslip edges on, and a 30° angled oval hole for holding the electrophoresis buffer vial, which consists of an Eppendorf tube (Hamburg, Germany) that has its top quarter removed at an angle. A platinum grounding wire (California Fine Wire Co., Grover Beach, CA) is placed in contact with the electrophoresis buffer, completing the circuit.
Trapping, manipulation, and injection of cells and beads are recorded by a monochrome CMOS camera (NT59-365, EO-1312M, Edmund Optics, Barrington, NJ) that is attached to the microscope housing with a 1× C-mount (Carl Zeiss, Jena, Germany).
An ancillary capillary was provided to clear the inlet to the separation capillary (and the trap) as needed by flowing high salt mGBSS at ~1 μL/min. The capillary position was controlled via another micromanipulator (Narishige Scientific Instrument Lab, Tokyo, Japan) placed to the left of the microscope; an HF-etched capillary (50 μm inner diameter, 360 μm outer diameter, and ~50 cm in length) was held by an acetal resin cylinder, similar to the one described above. PEEK fittings and a FEP sleeve were used to connect the capillary outlet to a syringe filled with high salt mGBSS. The pressure on the syringe was controlled by a syringe pump (model 601553, KD Scientific, Holliston, MA), located on top of the injection box.
Glass coverslips were used for all experiments except where noted. Several coatings and additives were tested to reduce adhesion of cells to the coverslip surface (as detailed in the Supplemental Information). Treated coverslips were stored at ambient temperature and humidity in Parafilm M (Pechiney Plastic Packaging Inc., Chicago, IL)-covered glass dishes, unless otherwise noted, until use.
Single pinealocytes were injected into the CE column for analysis. A 2.5 μL droplet of sample was pipetted onto the coverslip. The selected cell is held in the OT. The capillary inlet was directed into the cell's proximity by the micromanipulator, which was programmed to stop near the trap location and further position refinement was performed manually using the rotary optical encoder. Once the capillary was in place, the cell was released from the trap and hydrodynamic injection of the cell was performed by lowering the sheath waste outlet. Once injection was complete, the micromanipulator was used to bring the capillary inlet to the buffer vial, and the voltage and detectors were turned on. Injections were recorded using the CMOS camera on the microscope.
The sheath flow buffer was 25 mM citric acid, pH 2.25, and the flow rate was 0.2 mm/s for all experiments. The electrophoresis buffers and sample buffers varied as stated in the text and figure captions and both the capillary zone electrophoresis (CZE) and micellular electrokinetic chromatography (MEKC) modes were used. The voltage for all experiments was −30 kV unless otherwise stated. The injection volume varied as stated, but for bulk injections the volume was 14.7 nL for a 30 s hydrodynamic injection, which was achieved by lowering the sheath flow waste outlet by 32.5 cm. The typical laser pulse energy was between 1.5 μJ/pulse and 2 μJ/pulse.
The capillary was conditioned at the beginning of the day with 0.1 M NaOH for 15–20 min, followed by water for 5 min, and then electrophoresis buffer for a minimum of 5 min.
Data analysis was performed in IgorPro 5.05A (WaveMetrics Inc., Lake Oswego, OR). An automated data analysis script was written that reduces the user input to a single command. Output consists of four tables of calculated values with four corresponding color-coded graphs displaying the raw data, 6-point boxcar averaged data, normalized (with respect to the laser pulse energy) data, and both normalized and boxcar averaged data.
The baseline range (30 points, 10 s) with the lowest standard deviation was determined and used to calculate the limits of detection (LOD) for each PMT channel. Ratiometric analysis (calculating the intensity ratio between peak maxima in each of the PMT channels) was also automated to aid in analyte identification.
Single-cell analyte concentrations were calculated with an approach that removes background signals from the media and differences in separation conditions, as described here. The ratio of the injection lengths between a sample analyzed under cell lysing conditions and the same sample analyzed under non-cell lysing conditions was used to normalize the analyte concentrations under non-cell lysing conditions. These normalized values were used to calculate equivalent background concentrations, which were subtracted from the concentrations calculated under cell lysing conditions. The analyte amounts were then adjusted based on an assumed cell volume of 4 pL to represent the actual analyte concentration within the cell.
LODs and concentrations of analytes were determined by generating calibration curves for each analyte under the appropriate conditions. Investigated analyte concentrations ranged from the micromolar to the low nanomolar range typical for biological systems. The criterion for calculating the LODs was three times the standard deviation of the baseline.
In order to successfully trap and manipulate objects, the trapping laser should have a symmetric beam profile (usually Gaussian TEM00), low beam divergence, low ellipticity, and high pointing stability. Also, the NIR wavelength region is typically used for biological manipulations, as the NIR as a whole (from ~ 700 nm to 1300 nm) produces little damage to biological structures compared with visible and UV light. While water does not absorb NIR radiation, absorption of NIR radiation by endogenous chromophores makes some wavelength ranges better suited to these measurements than others.47,48 An Nd:YAG laser with 1064 nm emission was selected. No beam correction was needed, and the beam was expanded (by a factor of 20) to 8 mm to fill the back aperture of the objective, ensuring a steeper gradient and hence a stronger trap. The 1:1 telescope configuration was set up with the steering optic (plano-convex lens 1) located at a distance of 4f (1000 mm) from the back aperture. Translations in this lens correspond to movements of the trap, and were used to direct trapped objects to specific locations. The optical throughput of this system was experimentally determined to be 32% of the initial power, with the highest loss occurring at the objective. The trap size was ~1 μm, based on the numerical aperture of the objective and the wavelength of the laser. Polystyrene beads suspended in water were used to align the trap. A minimum initial power of 90 mW (power density ~6.5 × 106 W/cm2) was used to determine optimal conditions for the manipulation and translation of trapped beads.
The MC-CE-LINF instrument has several unique features; notable aspects include deep UV excitation, synchronization between the laser and PMTs, multiple detectors, a tapered outlet, post-column detection, and the ability to independently optimize the separation and detection conditions.
This excitation and detection system is controlled via the program provided by Photon Systems Inc. The laser pulse length and the PMT on-time are synchronized, resulting in reduced dark noise and low background signal. The net intensity for a given peak is determined in each channel and used to calculate three different ratios (green/blue or G/B, blue/red or B/R, and green/red or G/R). These ratios provide information on the unique spectral characteristics of the analytes and, when combined with migration time information, can allow for identification without using a complimentary technique or sample spiking, as would be required with a traditional single-channel system.
Post-column detection has been used for CE-LIF since the 1980s.49 For deep UV excitation, post-column detection is important because light scattering is proportional to λ−4. Another benefit of post-column detection is the ability to independently optimize the separation and detection conditions.50 This is useful because the maximal fluorescence for biogenic amines is achieved under acidic conditions but the best analyte separations use basic electrolytes. The HF-etched capillary outlet reduces background signal from scattering off of the edge of the capillary wall and changes the sheath flow profile around the outlet, allowing the excitation beam to be focused closer to the outlet.
To demonstrate the instrument's performance, the LODs for 13 indolamines known to be present in the pineal gland have been determined for both MEKC and CZE (Table 1) modes. The LODs are appropriate for measuring the physiological concentrations of the indoles present in pinealocytes.
Challenges that arose during single pinealocyte trapping and injection were adhesion to the coverslip and multiple cells and/or debris entering the capillary simultaneously. Pinealocytes can adhere to the surface of the glass coverslip within minutes after sample deposition, which makes selecting and trapping a cell for injection time-sensitive and can waste valuable sample. Once the cells are adhered, trapping is ineffective and moving the cells is difficult, even when using the capillary inlet to "push" them around the surface. Pinealocytes are robust and adhesion is strong; typically, pinealocytes do not lyse when they forcefully come into contact with the capillary inlet. Several coverslip coatings and solution additives were tested with pinealocytes to determine the best method to reduce adhesion, as detailed in the Supplemental Information. The efficacy of the coating was determined by three figures of merit: how easily a cell could be trapped and manipulated, and how long it took for cells to adhere to the coverslip. Based on the results of these experiments, poly(2-hydroxyethyl methacrylate)-coated coverslips were subsequently used for these studies.
Another issue is that multiple cells or cellular/tissue debris can enter the capillary during injection. We tried several approaches to minimize this from occurring. While one can prevent this is by using capillary out-flow (to push the untrapped objects away from the capillary inlet), this is effective only when the buffer is compatible with the sample. Here the electrophoresis buffer used for these experiments contained a surfactant to lyse the trapped cell once it entered the capillary. Using capillary out-flow under these conditions lysed the cells in the vicinity, including the trapped cell. We successfully used a second capillary, housed in and controlled by a second micromanipulator, to flush the area before injection of the undesired cells and debris. The second micromanipulator was manually adjusted and the second capillary's outlet was fitted to a syringe filled with high salt mGBSS with flow controlled via a syringe pump.
We used the OT-MC-CE-LINF system for single pinealocyte analyses, and bulk cell suspensions were analyzed with the MC-CE-LINF system. Two different CE conditions were used for these analyses: MEKC51,52 and CZE. Several of the indolamines of interest co-eluted without MEKC. The use of such distinct separation mechanisms allowed confirmation of analyte identity as it is less likely that the same analytes would coelute in both systems.
Prior to analysis, pinealocyte suspensions were split into two aliquots, referred to as incubated and untreated samples. Incubated samples were exposed to HTP to increase production of indolamines within the cells. This also tests for cell viability as only viable cells synthesize analytes of interest. The untreated samples were not incubated with HTP but rather an equal amount of high salt mGBSS was added to the suspension. Representative electropherograms from single pinealocyte injections under both incubation (Figure 2A) and untreated conditions (Figure 2B), respectively, from pineal samples acquired from the same animal are shown. Several analytes of interest have been detected and identified, such as serotonin 5-HT, NAS, HIAA, and TOL. In the HTP-incubated sample, elevated levels of 5-HT and HIAA are present compared to native levels (Figure 2A), as expected; a roughly 50-fold difference in 5-HT concentration is observed. HIAA is an enzymatic degradation product of 5-HT, so an increase in its concentration is also expected when elevated 5-HT levels are present. NAS is a product of 5-HT, the precursor to melatonin, and is present in low amounts relative to the other indolamines detected. The amounts of analytes present within an individual HTP-incubated pinealocyte correspond to 10−14 mol to 10−16 mol (mM to μM levels in a 10 μm diameter pinealocyte), an order of magnitude higher than the native levels present in an untreated pinealocyte. Two unknown indolamines (based on fluorescence properties) were also detected in the incubated sample (data not shown); work is ongoing to identify them.
Optical trapping is a versatile approach for manipulation of small samples such as individual cells and is well suited for positioning live cells adjacent to a CE capillary inlet. One can time cell injection with the physiological state of the cell under investigation. The current system works well when injecting and analyzing analytes from smaller mammalian cells such as the pinealocytes studied here. Future work will include investigating day- and night-time differences in pinealocyte content and chemical profile, as well as understanding the surprisingly complex indole chemistry within these cells.
Several modifications can be made to improve the performance and ease of use of the hyphenated instrument, including using smaller inner diameter CE capillaries and refining the second micromanipulator/capillary combination for improved injections. Using smaller inner diameter capillaries will reduce the likelihood of trapping and injecting multiple cells and reduce analyte dilution after cell lysis, enabling lower concentrations analytes within the cells to be characterized.
The authors would like to acknowledge the useful advice and assistance of Jennifer M. Arnold concerning the animal experiments. The help provided by Bill Hug, Ray Reid, and Prashant Oswal (at Photon Systems Inc.) was essential during the building of the analytical system. This work was supported by the National Institute of Neurological Disorders and Stroke under award number R01 NS031609 and by the National Institute of Dental and Craniofacial Research under award number R01 DE018866 and the National Heart, Lung, and Blood Institute under award number R01 HL086870. The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text:
Last night I got pretty drunk. Not falling over and stumbling around drunk but pretty freakin drunk. And I actually had a lot of fun. Until my boyfriend dragged me away and made me go home because I was embarrassing him in front of his friends.
My boyfriend told me I was being loud and I yelled at him infront of everybody and that his friends were making comments to him about how drunk I was.
I asked one of the girls I was around last night if I was really that bad and she said no.
I feel like I was the joke of the night and didn't even realize it. But everybody there was drunk too and I know all of them have been wayyyy worse than I was before. I feel like an idiot.
It's the morning after and I feel like a huge idiot. How can you get over drunk embarrassment?
Last night I got pretty drunk. Not falling over and stumbling around drunk but pretty freakin drunk. And I actually had a lot of fun. Until my boyfriend dragged me away and made me go home because I was embarrassing him in front of his friends.
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What Guys Said 1
Meh, we all have those nights. What probably happened was your boyfriend wasn't drinking or wasn't as drunk as you and was more easily embarrassed. If everyone is at generally the same level of drunkness then everything seems the same to everyone. Next time, make sure he is just as drunk as you and he will think it is also just as fun with no problems.00|00|0
How long do you think it will take for them to forget about it?
Usually once someone else does something a bit over the top.
What Girls Said 1
Well if I were you I'd dump him. You were drunk I was salty. If he gets embarrassed by you the rein he's supposed to care for and support then what exactly is he offering you in the relationship?
He's supposed tobuild I up not tear it down. He world is a cruel place to don't need cruelt in your personal life.
He's a selfish coward imp. I'd dump him. But that's me I have no problem cutting people out of my life if I detriment they don't respect me. And or are weak.
As for your night just accept you were drunk you acted drunk and it's over. Do what he should have done. Accept it have a chuckle give yourself an encouraging apt and move forward with your life.
Also he dragged you? You're an adult not a piece of meat. He can't force you to leave. If you were not hurting anyone or doing anything illegal it wasn't his place. That's just completely unacceptable.
For him to get hung up on it when it's over and doesn't even matter didn't hurt anyone and has nothing to do with anything going on now is just too cowardly for my taste. What would he do if there was a real problem. Plus he sounds like a party pooper. He should stay home next time.00|00|1
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Board Members
Home » Board Members
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|Steve Lindley
The Appraisal District Board of Directors Role in the Property Tax System
The local property tax system follows the principle of checks and balances. An appraisal district board of directors hires the chief appraiser, sets the budget and appoints the appraisal review board members.
The directors have no authority to set values or appraisal methods. The chief appraiser carries out the appraisal district's legal duties, hires the staff, makes the appraisals and operates the appraisal office.


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Shenzhen HQS Technology Co., Ltd.
Onsite Check
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Shenzhen HQS Technology Co., Ltd is professional manufacturer specializing in Sports Camera Accessor...
Shenzhen HQS Technology Co., Ltd is professional manufacturer specializing in Sports Camera Accessories, GoPro Accessories and Digital Peephole Viewers. You can see online at www.hqstec.com. We are the Gold Supplier on the alibaba.com for 7 years.
As an OEM/ODM service supplier, for the past 4 years, HQS has accepted various kinds of OEM/ODM orders primarily from overseas markets with the excellent qualifications below:
1.Strong R&D ability(No. of R & D staff:10)
2.Effective production team
3.Strict quality control(No.of QC staff:10)
4.Competitive product price
5.Intellectual property protection
6.Sincere customer service
7.Our products and service reach buyers in Europe,the Americas, Southeast Asia and Ausstralia. Repeated orders testify our trusted products and service. 90% of our clients keep placing repeated orders in the last 4 years.
8.From fantabulous high quality to strictly quality control to professional after-sell service, HQS keeps the promise to produce the world-class products.
As one of the top digital products design and produce company, we have set up "HQS" brand as a top status among the middle and top market. Our complete management organization includes such departments as high-quality design and development, produce, promotion, and after-sales service. On the basis of dealing with ODM and OEM orders, we design, produce and control quality strictly according to the UL, CE, FCC and CCC standards.
With the operational principle of "innovation and quality", we always supply to our customers good quality video products and thoughtful services in order to establish mutual beneficial, stable and continuously development long-time cooperation.
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Trade & Market
|Main Markets
|Total Revenue(%)
|South Asia
|Southern Europe
|Northern Europe
|Western Europe
|Eastern Asia
|Mid East
|Southeast Asia
|Eastern Europe
|South America
|North America
|Export Percentage:
|81% - 90%
|Export Mode:
|Using an agent
|No. of Employees in Trade Department:
|21-50 People
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I got into a city bus on my way home, and looked to the back to see any of the good spots are open for sitting for me.
Before I sat down at the front, I noticed this girl in the back looking at me. She must have saw me when I first came on the bus.
Later on, I looked to the back of the bus to look at the people leaving the bus and see if there is any spots left. She had fixed look on me. I have a feeling she was staring at me.
Why did she give me eye contact on the bus?
I got into a city bus on my way home, and looked to the back to see any of the good spots are open for sitting for me.
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What Girls Said 1
She might have found you attractive or intriguing. Or she was deep in thought.11|00|0
Let me say now that girls do like attention and seeing a handsome guy in her league/age range who was looking at/near her would spark her interest. She looked at you and tried to bridge eye contact to show interest. Next time you see this, stare back at her for 3 seconds and don't look at her wierdly, if she looks away, then it was because she was trying to recognize you or something else.
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Day Two of the 23rd European Lutheran Conference Discusses Living in an Ecumenical Context
Communication and Projects Coordinator for Eurasia, Rick Steenbock, reports on the activities of the second day of the 23rd European Lutheran Conference (ELC).
May 23, 2014, Bleckmar, Germany-
In a word, Day Two of the European Lutheran Conference was about practices. Following morning devotion and Bible study, once again the focus turned to living in an ecumenical world and how do we as confessional Lutherans "practice" this in day-to-day ministry. The goal of this conference was not only to discuss what it means to live in an ecumenical world but also to discuss and develop guidelines that congregations can use in deciding what is proper and what is not.
Rev. John Ehlers, chairman of the Evangelical Lutheran Church in England (ELCE), presented a paper on how the ELCE has dealt with some of the ecumenical issues over the past decade or so. He gives a brief overview of his paper here:
The conference then discussed his paper and broke into small groups to discuss all they had heard over the last two days.
The whole group reconvened to share ideas and opinions. On the last day, they will vote to adopt or decline these recommendations.
One last piece of business wrapped up the evening. The steering committee for the 24th European Lutheran Conference was elected. President Rev. Klaus Pahlen of Germany, vice-president Rev. Leif Jensen of Denmark and secretary Rev. George Samiec of England will lead the next conference that convenes in two years.
Tags: 23rd European Lutheran Conference, Denmark, ecumenical, ecumenism, ELCE, England, Germany, Practice, Rev. John Ehlers, Rick Steenbock


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Free Shipping Over $75* Shop Our Back to School SaleWhy Shop Eurway.com?
Pretty in pastels, the Gumdrop Rug celebrates aqua, green, purple, and grey. The playful design is simple yet sophisticated with a light antique wash adding a bit of depth to the beautifully light colors. Hand-tufted and hand-carved of 100% blended wool, Gumdrop is a youthfully modern rug that is durable and designed to stand the test of time.
Learn more about rug sizing with our rug buying guide.
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Global Sugar Art Discount?
Decorating By CakesbyMonica Updated 26 Jul 2008 , 5:38am by CakesbyMonica
Forgive me if I'm wrong...did it say at one point CC'ers get a % discount from Global Sugar Art? Perhaps it was a limited time thing?
3 replies
I haven't heard about a discount, but Cake Central is supported when you purchase something from them.
You know, I was just thinking maybe if you post this question in the Global Sugar Art forum, someone who really knows might answer.


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First Tutors: Music is the best way to locate Thornton-cleveleys tuba teachers in the UK, helping you discover Thornton-cleveleys tuba lessons close to you and Thornton-cleveleys tutors to assist with any instrument like piano, violin or guitar. We can help you find Thornton-cleveleys tuba lessons or a Thornton-cleveleys tuba teacher to prepare for tuba grades and develop your tuba skills! Our search will order Thornton-cleveleys tuba tutors local to you according to their hourly prices and distance from you.
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SIMPLE MAN "Classic" Snapback Cap
Posted by Maui, June 15th, 2012 06:06 am under News
Established in 2010, SIMPLE MAN is a "line of clothing that makes deciding what to [wear] simple." Inspired by the culture of Toronto, Canada, SM's collection easily transcends across the Canadian borders.
As shown above, this "Classic" snapback cap is simple with an LA Dodgers color pattern. Blue and White. The backdrop takes Blue with the windows and the front embroidery is White. Want one? Get your HERE.


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New stage at Dour Festival 2017: La Caverne!
The plot thickens! After weeks of new additions in hip hop, reggae, world and electronic music, we'd like to take some time to please the rockers among us!
How to please them better than to introduce a new stage: La Caverne ("the cave") is the result of long reflections on the rock line up of the festival. An exclusive rock stage where guitars will prevail all day long, from 13h till long after dark.
Let's start off with The Kills , with the divine Alison Mosshart who recently released their brilliant 5th album. Temperatures will continue to rise with the American Deap Vally and the sexy Californian Hanni El Khatib.
Furthermore, some sublime nonchalance with Twin Peaks, furious pop music with Circa Waves, bedazzling fuzz with Meatbodies, retro rock and rhythm'n'blues with the Irish The Strypes and the psychedelic 60s and 70s trip of All Them Witches.
Everything will go at La Caverne, like the sludge and doom metal of AmenRa, chaotic hardcore of Oathbreaker, post black metal of French Alcest and baroquecore of Igorrr.
The 29th edition of Dour Festival will take place from 12 until 16 July. Tickets and Festivillage packages are on sale at tickets.dourfestival.be


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Singapore's Keppel Corp. Ltd. reported Wednesday that Keppel AmFELS, a unit of its wholly owned subsidiary Keppel Offshore & Marine Ltd. (Keppel O&M) in the U.S., has delivered one of the world's largest harsh environment enhanced mobility land rigs 41 days ahead of schedule.
"The early and safe delivery of this rig is a result of the strong execution capabilities of the yard and close teamwork with our customer. Even though it is the first land rig of its type to be built by Keppel, we are able to leverage the experience and expertise of Keppel O&M to undertake such complex projects and deliver it to the satisfaction of our customer. It extends the track record of Keppel AmFELS as the leading rig building yard in the Gulf of Mexico," President of Keppel AmFELS Simon Lee said in the press release.
The workscope for Texas's Brownsville-based Keppel AmFELS included engineering, procurement and construction of the rig.
Keppel AmFELS' other ongoing projects includes the construction of a KFELS B Class jackup for Mexican drilling company, Perforadora Central.
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Allergies can be a pain to deal with. They can be caused by pretty much anything, with the most common ones being allergy to dust, pollen, and cat hair. For people who have a tough time dealing with these allergies during certain season, it can be exhausting to go about your daily life having trouble breathing and dealing with all of those unpleasant symptoms. We asked ENT doctors about the best ways to deal with allergies, even when they are at their worst, and this is what they recommended.
- Cleaning Routine: If you suffer from allergies, the best thing you can do for yourself is to have a cleaning routine in your home. It is most effective when you clean every week, to remove dust, dirt, and other allergens. Be sure to always use a wet mop so that the dust does not spread into the room. Also, be sure to use chemicals that are made for allergy suffers, since harsher options can irritate your lungs and nasal passages.
- Pollen Count: If you are someone who is allergic to pollen, the best thing to do is to be as prepared as possible. You cannot avoid pollen completely, but if you know when the pollen count will be higher, you can be ready for it. There are many apps that tell you the pollen count and you can even set up alarms to tell you when it is higher than normal. This is one thing that all ENT doctors recommend.
- Be Careful Where You Walk: If you are someone who enjoys jogging or walking outside, allergies can be a concern. The best thing you can do is to choose residential areas instead of commercial areas, since the car exhaust will be less. Parks are great, as well, unless you have plant allergies or pollen allergies. Stick to residential areas and you will have a much easier time of it.
- Nasal Sprays: A great way to help yourself during allergy season is to use a nasal spray regularly. This helps flush out any dust or debris from your nose and nasal passages, making it less likely that you will suffer an allergic attack. Take the time to read up on the different spray options available to ensure that you choose the right one.
- Medication: Many people turn to medication that they can use the whole year to protect them against allergies. If you suffer from allergies and you want to be rid of the worst of the symptoms, having a great medication on your side can make a huge difference.
All of these tips can make dealing with allergies much easier. A clinic like Kaplan Sinus Relief is well known for being able to provide patients with the help they need when allergies strike. Take the time to read about and choose a great clinic with good doctors who will be able to give you the relief from allergies that you need to get on with your life without serious interruptions to your routine.


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Kincumber Public School
by: Skoolbag • 1
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by: Skoolbag • 1
Kincumber Public School Skoolbag App for parent and student community. Download this App to be kept up to date with everything that is happening at KPS. It features Events, News, School Enews Newsletters, Documents, and push notification alerts direct from the school.
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Happy Tuesday everyone!
This past weekend the husband and I hosted our first iftar (breaking the fast) party at our house. We cooked traditional Pakistani iftari food and the quintessential Pakistani dinner.
I even got festive and found this free printable DIY banner online from an website called Sakina Design, they feature other cute printable for stars and beautiful Islamic art frames that I'm dying to get. The banner was a hit and gave me ideas to get more creative with the decor for future iftaries/parties.
The menu for iftari consisted of fruit chaat, cholay, spinach pakoray, aloo pakoray, and per my husbands request finger pepper pakoray which weren't spicy to our surprise.
We also had dahi baray, chicken samosas (bought frozen from Tahoora), ordered some pizza for the kids, coriander chutney and homemade tamarind chutney.
Of course we had some fresh watermelon, cantaloupe, honeydew melon, apple juice, and rooh afza, the summer drink of the east which every iftari has to have otherwise your thirst doesn't feel quenched.
Everyone was stuffed from the iftari but we still had dinner, spicy biryani made by the husband (the man can cook some yummy food Alhmd), and chicken tikka made by me. The main dinner was accompanied by salad and yogurt raita.
And last but not least, the iftari party had a sweet ending with some light summer mango & pineapple dessert.
We enjoyed hosting our 1st ever iftari party. While it was a lot of work, insha'Allah (God willing) we will host future parties with more family and friends. Until then I hope all my Muslim family and friends are having a blessed Ramadan and insha'Allah we can all make the most of the last ten days, and may our fasts & ibadah (supplications) be accepted ameen.
Have you hosted any parties? What are some traditional items you cook?
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