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It's Friday! The only thing that makes me happier than is if someone would grocery shop for me then load it in my car for me, BUT wait...that happened! What????
This week in blogging:
Monday: My blog buddy Jacquelyn nominated me for the Liebster award. Check out my post to find out more random facts about me.
Tuesday: I have a few special friends that have said goodbye to their sweet children too early. Tuesday's post was dedicated to them in the midst of their grieving, and what we can do for them.
Wednesday: I reviewed the new (new to me) grocery pickup at WalMart. If you missed it, definitely check out that post. It is a game changer.
A couple of weeks ago I saw the table below listed for $25 on an online garage sale site and had to have it. Jess had a bit of an accident this fall on our not-so-sturdy patio chairs that resulted in staples to the back of his head, and the chairs being trashed ASAP! So, we were in need of a new set, and I am all about a bargain.
This is a sturdy, wrought iron set that just needed a little TLC.
I just knew I wanted to paint it Seaside Blue (aka turquoise), and he was like...what? A blue table?
And sister, was all, "What are you thinking, Mommy?"
But, seven cans of spray paint later (and yes, if you're doing the math in your head,we spent more on the paint than the table) we have a finished product. I am still shopping for the perfect seat cushion. I have been to no less than 20 stores this week to find the right one, but it hasn't happened yet. I'm thinking coral and white...
THREEJess' preschool had their annual Round-Up, which is basically a fundraiser dinner where the kids perform. I loved watching him sing and dance. It was so fun! I love his little personality.
He was actually "whipping" here (whip, nay, nay), is that not the most serious whip, you have ever seen?
FOURJess' preschool also holds a live auction after the performance. I initially said, I did not need a bidding number, because I had not planned on purchasing anything, but thank goodness I changed my mind. The first item up was this beautiful cutting board. We needed a new wooden cutting board because I put mine in the dishwasher. I know what you're thinking...wooden cutting boards don't go in the dishwasher. What I didn't know was that you aren't suppose to cut raw chicken on a wooden cutting board. When my husband found out I had done that he
It may come as a shock to those of you that know I have been eating the Trim Healthy Mama way since August that I just now bought the cookbook! I have the original book that explains the plan and some recipes, but I just got the "official" cookbook this week. I LOVE it! So far this week I have made the Lazy Lasagna, Cajun Chicken, Tilapia Veracruz and am planning the Chicken Bang Bang tonight. They were all delicious and even my sometimes picky kids ate it up. If you are looking for some new ideas that are healthy and family friendly, I definitely recommend this book (I just had a Reading Rainbow flashback, anyone else?) Right now, this book is under $16 on Amazon.
Thanks for spending a little time with me this week. I hope you have a fantastic weekend!
P.S. This post contained affiliate links, which means if you clicked on a link & made a purchase, I may have received a little commission. Thank you for reading and supporting my blog.
Linking up with these ladies:


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Warning: By law we cannot sell this product to anyone under 18, by placing an order with us you are confirming that you are over the age of 18.
Whiskas Temptations Cat Treats with Beef 60g
£1.25 £2.08 per 100g
This is a specialist veterinary product. We will ask you a simple set of questions to confirm that you are buying the correct medicine for your pet and know how to use it safely
Order by 3pm for Express Courier Delivery Some of the items we sell are a bit too bulky to fit on our delivery vans so we offer our customers Next Day Delivery via courier. Deliveries are made from 8am - 8pm Monday to Saturday and 9am - 5pm on Sundays. Your delivery slot will only be confirmed once your order has been placed.
Whiskas® Temptations are delicious cat treats with a tasty crunchy outside and an irresistible soft centre. With a range of mouth-watering flavours, containing extra vitamins and minerals, and with less than 2 Kcal in every treat, they are a great way to give your cat a treat they'll love.
Key Benefits
Delicious dual textured cat treats with a tasty crunchy outside and an irresistible soft centre., Prepared in delicious varieties, including tasty chicken & cheese, beef, salmon, seafood and turkey, because your cat naturally seeks a varied diet, Less than 2 Kcal in every cat treat, Cat biscuits with added vitamins and minerals and no artificial flavours
Product Information
WALTHAM™ The World's Leading Authority on Pet Care and Nutrition
Cereals, Meat and Animal Derivatives (including 4% Beef), Oils and Fats, Minerals
|Typical Values
|Analytical Constituents (%):
|Fat content:
|Inorganic matter:
|Crude fibres:
|Additives per kg:
|Antioxidants and Colourants
|Nutritional additives:
|Vitamin A:
|Vitamin B3:
|50.5 mg
|Vitamin B6:
|45.6 mg
|Vitamin B9:
|1.3 mg
|Vitamin D3:
|Cupric sulphate pentahydrate:
|26 mg
|45.4 mg
|45.4 mg
|Potassium iodide:
|2.2 mg
|Zinc sulphate monohydrate:
|202 mg
Feeding instructions: Feed up to 11 pieces per day. Please reduce main meal accordingly. Use within 4 weeks of opening. Fresh drinking water should always be available.
Feeding Guide
Fresh drinking water should always be available.
Feed as a treat at any time of the day
Feed as a treat at any time of the day

subset: commoncrawl


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- Over 18 years of trusted, clinically proven pain relief.
- Backed by scientific research done by Dr. Stuart McGill, director of spine biometrics at the University of Waterloo.
- Tens machines used worldwide to treat pain, from pro-athletes to at home use.
The Circulation Promoter is designed to help relieve discomfort and pain in the legs and feet. Powered by the Pain Therapy System*, which is clinically tested to increase blood and nerve circulation and decrease muscle tension, the Circulation Promoter will not require any external power source.
The complete system combines Transcutaneous Electrical Nerve Stimulation (T.E.N.S) and Electrical Muscle Stimulation (EMS) to help stimulate the nerves and muscles in the soles of the feet. The foot pads are set on a base so that users can benefit from treatment no matter where they are. With foot pads divided into targeted sections as well as extra-conductive pads in the heel and arch areas, the Circulation Promoter will provide more consistent and comfortable stimulation.
- Helps relieve discomfort and pain in the legs and feet
- Clinically tested to increase blood flow and nerve circulation
- Engineered with TENS and EMS to help stimulate muscles
- A great way to relax your feet and legs after a hard day
- Circulation Promoter
- Pain Therapy 2-Pad System
- Small Massage Pads
- Large Massage Pads
- Small Replacement Massage Pads
- Spray Water Bottle for Convenience
- Instruction Manual, DVD & Travel Case


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New York tabloids take Patriots QB Tom Brady's comments and have, um, a party
Yesterday at a raucous pep rally, Patriots QB Tom Brady made the seemingly innocuous statement that he hoped there could be more fans at a party next week. Ya know, in case they win. And when asked about it again yesterday in Indy, Brady quipped, "Uh, it was a pep rally." Nope, New York papers took it a little further in a classic case of being hilarious. They are stepping up their game for the Super Bowl. Check out the back pages:


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About the Book
Images taken of flowers from my backyard. This project was to show that you don't have to go somewhere else to take beautiful images, beauty is all around even right outside your own door.
Features & Details
- Category Fine Art Photography
Standard Landscape, 10×8 in, 25×20 cm
46 Pages
- Publish Date Oct 24, 2008
- Tags flowers, abstract
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Systemic iron homeostasis is mainly controlled by the liver through synthesis of the peptide hormone hepcidin (encoded by Hamp), the key regulator of duodenal iron absorption and macrophage iron release. Here we show that the liver-specific microRNA miR-122 is important for regulating Hamp mRNA expression and tissue iron levels. Efficient and specific depletion of miR-122 by injection of a locked-nucleic-acid–modified (LNA-modified) anti-miR into WT mice caused systemic iron deficiency, characterized by reduced plasma and liver iron levels, mildly impaired hematopoiesis, and increased extramedullary erythropoiesis in the spleen. Moreover, miR-122 inhibition increased the amount of mRNA transcribed by genes that control systemic iron levels, such as hemochromatosis (Hfe), hemojuvelin (Hjv), bone morphogenetic protein receptor type 1A (Bmpr1a), and Hamp. Importantly, miR-122 directly targeted the 3′ untranslated region of 2 mRNAs that encode activators of hepcidin expression, Hfe and Hjv. These data help to explain the increased Hamp mRNA levels and subsequent iron deficiency in mice with reduced miR-122 levels and establish a direct mechanistic link between miR-122 and the regulation of systemic iron metabolism.
The liver is the major body iron storage site and the endocrine organ responsible for the regulation of systemic iron homeostasis. The homeostatic system controls plasma iron availability in order to supply iron to cells and tissues and to prevent toxic iron excess. It reacts to the demand of the erythron, which requires most of the systemically available iron for erythroid heme synthesis and transmits signals to duodenal enterocytes (which absorb iron from the diet), macrophages (which release iron recycled from senescent erythrocytes), and hepatocytes (the major iron reserve; ref. 1). The liver orchestrates iron fluxes by synthesizing the small peptide hormone hepcidin (encoded by Hamp; also known as LEAP1; refs. 2, 3), which controls the amount of iron available in the circulation by interacting with the iron exporter ferroportin. Ferroportin is expressed on the surface of duodenal enterocytes, macrophages, and hepatocytes and upon hepcidin binding is internalized and degraded (4). Disturbance of the hepcidin/ferroportin regulatory system unbalances systemic iron levels and causes important disorders, such as iron deficiency anemia (e.g., iron refractory iron deficiency anemia; ref. 5) or iron overload disorders (e.g., the frequent genetic disorder hereditary hemochromatosis [HH]; ref. 6), or contributes to the iron overload of iron-loading anemia, such as the thalassemias (7). The liver senses systemic iron availability through genes mutated in HH — hemochromatosis (Hfe; refs. 8, 9), hemojuvelin (Hjv; refs. 10–12), and transferrin receptor 2 (Tfr2; refs. 13, 14) — and through the bone morphogenetic protein 6 (15, 16) and Smad4 proteins (17, 18), which serve to regulate hepcidin transcription. In addition, inflammatory cytokines secreted from macrophages stimulate hepcidin synthesis, resulting in iron retention in macrophages (e.g., hepatic Kupffer cells) and reduced intestinal iron absorption. Persistent induction of hepcidin by macrophage-secreted inflammatory cytokines results in the anemia of inflammation, a common disorder of patients with chronic inflammatory conditions (19).
MicroRNAs (miRNAs) are an abundant class of short, noncoding RNAs that regulate gene expression at the posttranscriptional level. miRNA activity has been associated with the control of a wide range of processes, such as development, differentiation, and metabolism (20). Mature miRNAs interact with the RNA-induced silencing complex to base pair with partially homologous sequences in the 3′–untranslated region (3′-UTR) of target mRNAs. Mechanistically, miRNAs prevent mRNA translation (21) and/or mediate target mRNA degradation (22). Computational analyses suggest that miRNAs may regulate up to 30% of the human protein-coding genes (23); however, thus far only a small number of target genes have been experimentally confirmed (TarBase database of validated miRNA targets; ref. 24).
miR-122 is an abundant, liver-specific miRNA (25, 26) whose expression is decreased in advanced liver diseases, such as cirrhosis (27) and hepatocellular carcinoma (28, 29). Several independent studies demonstrate that in vivo inhibition of miR-122 reduces systemic cholesterol levels by as-yet unidentified molecular mechanisms (30, 31). We noticed that these publications report increased mRNA levels of the HH-associated proteins Hfe and Hjv in response to miR-122 inhibition (31, 32). Moreover, miR-122 expression is critical for HCV infection, replication, and response to IFN therapy (33–35). Taken together, these data indicate that miR-122 plays a fundamental role in regulating hepatic functions.
In this study, we addressed the question of whether miR-122 is involved in maintaining systemic iron homeostasis. We showed that expression of miR-122 was decreased in a mouse model of HH and in liver biopsies of HH patients. Efficient inhibition of miR-122 in WT mice caused systemic iron deficiency, hallmarked by reduced plasma and liver iron levels, impaired hematopoiesis, and increased extramedullary erythropoiesis. Moreover, miR-122 depletion directly increased mRNA expression of activators of hepcidin transcription, such as Hfe, Hjv, and bone morphogenetic protein receptor type 1A (Bmpr1a), in liver tissue and murine primary hepatocytes. Elevated hepcidin levels help to explain the systemic iron deficiency in mice with reduced miR-122 levels.
To investigate whether miRNA expression is altered in response to hepatic iron overload, we analyzed total liver RNA from mice with primary iron overload as a consequence of Hfe deficiency (36) or WT mice with iron overload caused by iron-dextran injection (8). miRNA expression profiling was performed using the miChip microarray platform (37–39). We found that miR-122, a miRNA abundantly and selectively expressed in the liver, was downregulated in Hfe–/– mice (data not shown). Subsequent real-time quantitative PCR (qPCR) analysis revealed significantly reduced hepatic miR-122 expression in untreated Hfe–/– mice compared with WT controls (1.63-fold; P = 0.039; Figure Figure1A),1A), but not in WT mice injected with iron-dextran (P = 0.187; Figure Figure1B)1B) or subjected to dietary iron overload (P = 0.471; Figure Figure1C).1C). Similar to the data obtained in Hfe–/– mice, we observed reduced miR-122 levels in liver biopsies from HH patients with homozygous C282Y mutations compared with control subjects without Hfe mutations or iron overload (1.68-fold; P = 0.068; Figure Figure1D).1D). However, statistical significance was not reached. Interpretation of the reduced miR-122 levels in HH patients is hampered by the fact that in addition to Hfe deficiency and iron overload, HH patients experience varying degrees of hepatic fibrosis and steatosis, as well as therapeutic interventions that may affect miR-122 expression (refs. 35, 40, and Supplemental Table 5; supplemental material available online with this article; doi: 10.1172/JCI44883DS1). In contrast, Hfe–/– mice do not demonstrate hepatic fibrosis or fat accumulation (41), which suggests that the lack of Hfe specifically causes decreased miR-122 levels. These data, together with previous observations that Hfe and Hjv mRNA expression increased in the livers of miR-122–depleted mice (31, 32), led us to hypothesized that miR-122 could be involved in maintaining iron homeostasis.
To functionally investigate a possible link between miR-122 and iron metabolism, we inhibited miR-122 by a single i.p. injection of locked nucleic acid–modified (LNA-modified) anti-miR oligonucleotides (31) into age- and sex-matched C57BL/6 WT mice. To inhibit miR-122 specifically, we injected an anti-miR compound with perfect complementarity to miR-122 (perfect match; referred to herein as PM_anti–miR-122). As negative controls, mice were injected either with an LNA control compound with 2 mismatches (referred to herein as 2MM_antimiR-122) or saline vehicle control (0.9% NaCl). Mice were sacrificed 3 and 6 weeks after injection. Independent of treatment, mice were viable and exhibited no overt physical or behavioral abnormalities. To assess the efficiency of miR-122 inhibition, hepatic miR-122 levels were measured by qPCR (Figure (Figure2A).2A). The amount of detectable miR-122 was reduced compared with saline-injected mice by 28- and 11-fold at 3 and 6 weeks, respectively, after injection with PM_anti–miR-122. Injection of the 2MM_anti–miR-122 control did not significantly reduce miR-122 detectability. Expression of the miR-122 primary transcript was not altered under the experimental conditions (Supplemental Figure 1A). To exclude that PM_anti–miR-122 administration disturbs the expression of other miRNAs we analyzed miRNA expression profiles in the livers, hearts and spleens of the same mice (Supplemental Figure 2). Our data show specific and exclusive inhibition of miR-122 in the liver of PM_anti–miR-122 treated mice. In the spleen, expression of 3 miRNAs was increased in PM_anti–miR-122–treated mice, which may be a consequence of increased extramedullary hematopoiesis (see below). Alteration of the miRNA profile was not detected in the heart, where miR-122 is not expressed (26).
Furthermore, miR-122 inhibition by PM_anti–miR-122 increased hepatic mRNA levels of the miR-122 target gene aldolase A (Aldoa; ref. 31) at both 3 weeks (2.8-fold vs. saline; 4.0-fold vs. 2MM_anti–miR-122) and 6 weeks after treatment (3.4-fold vs. saline; 2.7-fold vs. 2MM_anti–miR-122; Figure Figure2B).2B). Likewise, serum cholesterol levels were decreased (30, 31) 3 weeks after injection of PM_anti–miR-122 (Supplemental Figure 1B). Taken together, these data suggest that PM_anti–miR-122 injection specifically and efficiently antagonizes miR-122 and that miR-122 inhibition is functional.
To assess potential consequences of miR-122 inhibition on systemic iron metabolism, we analyzed hematological parameters in PM_anti–miR-122–injected mice (Table (Table11 and Supplemental Table 1). The mean corpuscular volume (MCV) of erythrocytes was significantly decreased 3 and 6 weeks after PM_anti–miR-122 administration compared with 2MM_anti–miR-122– or saline-injected mice. In addition, reticulocyte counts were significantly increased at the 3-week time point. Reticulocyte hemoglobin (Rhb) content, a useful diagnostic parameter for iron deficiency (42), was significantly reduced in PM_anti–miR-122–treated mice 3 weeks after injection (Table (Table1).1). Whereas elevated reticulocyte numbers suggest ongoing hemolysis in humans, reticulocytosis in mice arises as a consequence of dietary iron deficiency (43). Taken together, the hematological analyses of PM_anti–miR-122–treated mice showed that miR-122 inhibition hinders hematopoiesis, possibly through inducing mild iron deficiency.
We next analyzed the iron content of the liver (site of iron storage), spleen (site of iron recycling), and plasma (site of iron transport) in miR-122–depleted mice (Table (Table2).2). Interestingly, the non-heme iron content was markedly decreased in the liver of PM_anti–miR-122–treated mice both 3 and 6 weeks after injection (3 weeks, 18% vs. saline and 10% vs. 2MM_anti–miR-122; 6 weeks, 27% vs. saline and 24% vs. 2MM_anti–miR-122). In contrast, a small but significant increase in iron content was observed in the spleen of PM_anti–miR-122–treated mice at the 3-week time point compared with both controls (49% vs. saline and 19% vs. 2MM_anti–miR-122); this was normalized 6 weeks after injection. Consistent with iron-deficient hematopoiesis, we observed a decrease in plasma iron levels (approximately 12.5% vs. saline and 17% vs. 2MM_anti–miR-122) and transferrin iron-binding capacity (Supplemental Table 1) at the 3-week time point. Interestingly, plasma iron levels started to increase in PM_anti–miR-122–treated mice 6 weeks after treatment (Table (Table2),2), which suggests that the slight recovery in miR-122 activity at the 6-week time point may be sufficient to trigger this effect. Alteration of both hematological parameters and tissue iron levels that occur in response to in vivo miR-122 depletion are supported by data from independent experiments that were conducted under modified experimental conditions or by using WT mice on a different genetic background (Supplemental Table 2). Taken together, these results indicate that in vivo inhibition of miR-122 decreases systemic iron and that an inadequate iron supply to the erythron mildly impairs hematopoiesis.
Systemic iron levels are sensed by genes mutated in hereditary hemochromatosis (i.e., Hfe, Hjv, and Tfr2), and the signal is transmitted, at least in part, via the Bmp/Smad signaling pathway to regulate transcription of hepcidin (44–47). To investigate whether mRNA expression of genes in this pathway is affected by in vivo miR-122 inhibition, we analyzed total RNA from the liver of PM_anti–miR-122–treated mice. mRNA expression of Hamp was significantly increased in PM_anti–miR-122–treated mice 3 weeks after injection compared with 2MM_anti–miR-122– and saline-injected controls (1.96- and 2.3-fold, respectively; Figure Figure3A).3A). In addition, mRNA expression of 3 positive hepcidin regulators, Hfe (3.9-fold vs. saline; 2.9-fold vs. 2MM_anti–miR-122), Hjv (7.1-fold vs. saline; 3.8-fold vs. 2MM_anti–miR-122), and Bmpr1a (4.9-fold versus saline; 2.8-fold vs. 2MM_anti–miR-122), was significantly increased in PM_anti–miR-122–injected mice. In contrast, we did not detect any PM_anti–miR-122–specific change in the mRNA expression of Tfr2 or other hepcidin effectors (e.g., Smad7 and Smad4; Supplemental Figure 3). Consistent with the partial relief of miR-122 inhibition (Figure (Figure2)2) and increased plasma iron levels (Table (Table2)2) at the 6-week time point, Hamp and Hfe mRNA expression returned to normal, and the changes in mRNA levels of Hjv (2.4-fold vs. saline and 4.1-fold vs. 2MM_anti–miR-122) and Bmpr1a (1.9-fold vs. saline and 2.2-fold vs. 2MM_anti–miR-122) diminished at 6 compared with 3 weeks.
To investigate whether increased mRNA expression of Hfe, Hjv, Hamp, and Bmpr1a in the liver of PM_anti–miR-122–treated mice is a consequence of hepatocyte-specific miR-122 depletion, we transfected murine primary hepatocytes with either PM_anti–miR-122 or scrambled control oligos. Consistent with the observations in PM_anti–miR-122–treated mice, we found significantly increased mRNA expression of Hfe (24 hours, P = 0.026; 48 hours, P = 0.117), Hjv (24 hours, P = 0.014; 48 hours, P = 0.11), Hamp (24 hours, P = 0.005; 48 hours, P = 0.044), Bmpr1a (24 hours, P = 0.034; 48 hours, P = 0.175), and Aldoa (24 hours, P = 0.003; 48 hours, P = 0.119) in primary hepatocytes (Figure (Figure4). 4).
In principle, LNA oligonucleotide–mediated activation of proinflammatory cytokines, such as IL-6, could stimulate Hamp mRNA expression via the Jak/Stat signaling pathway (19). Therefore, we analyzed mRNA expression of the inflammatory marker Il1b as well as Il6 and its downstream effector C-reactive protein (Crp) in PM_anti–miR-122–injected mice (Supplemental Figure 4A). Consistent with the lack of increased Il6 mRNA expression in PM_anti–miR-122–injected mice, we did not observe increased phospho-Stat3 levels in liver protein extracts (Supplemental Figure 4B), which suggests that the Jak/Stat signaling pathway is not activated in these mice. Moreover, phosphorylation of Smad1/5/8 and mRNA expression of DNA binding protein 1 (Id1), a downstream target gene of the Bmp/Smad signaling pathway (48), remained unchanged in the liver of PM_anti–miR-122–injected mice (Supplemental Figures 3 and 6). Analyses of liver morphology, as well as assessment of T cell and macrophage numbers by immunohistochemistry, did not detect any significant differences between PM_anti–miR-122–treated mice and respective controls (Supplemental Figure 4C). Taken together, these data show that hepcidin stimulation in PM_anti–miR-122–injected mice is unlikely the result of an inflammatory response mediated by IL-6 or IL-1β, which indicates that miR-122 directly or indirectly controls expression of one or more of the activators of Hamp levels (i.e., Hfe, Hjv, or Bmpr1a) or of Hamp itself.
To investigate whether murine Hfe, Hjv, Bmpr1a, or Hamp are targets for miR-122, we queried several miRNA target prediction databases: miRanda (49), PicTar (50), miRTar ( http://mirtar.mbc.nctu.edu.tw/human/), TargetScanS (51), and TarBase (24). Applying this strategy, we identified 3 putative miR-122 binding sites in the 3′-UTR of Hjv and a single putative miR-122 target sequence in the 3′-UTR of Hamp mRNA (Supplemental Table 3). Application of the RNA22 algorithm (52) predicted 2 miR-122 binding sites in the 3′-UTR of the Hfe gene (Supplemental Table 3). No miR-122 seed sequences were identified within the Bmpr1a 3′-UTR; therefore, Bmpr1a was not further analyzed. We generated luciferase reporter genes bearing the full-length 3′-UTRs of mouse Hamp, Hfe, and Hjv (referred to herein as pMIR-mHamp, pMIR-mHfe, and pMIR-mHjv, respectively). As a positive control, we used the 3′-UTR of the mouse Aldoa gene (pMIR-mAldoa), a validated miR-122 target (31). As a negative control, the entire 3′-UTR of mouse Gapdh (pMIR-mGapdh) was inserted. To assess the specificity of putative miR-122 regulation, we also generated constructs where the seed sequences of the miR-122 binding sites were mutated (Supplemental Table 3; bold sequences). To further monitor expression control by miR-122 mimics (Ambion), we generated artificial positive and negative control vectors containing sequence with perfect complementarity to miR-122 and to the miR-122 sequences directly (pMIR-122+ and pMIR-122–, respectively).
To investigate whether the predicted 3′-UTRs of the Hfe, Hjv, and Hamp mRNAs convey miR-122–dependent regulation, we cotransfected miR-122 mimics (referred to herein as pre–miR-122) and the luciferase reporter plasmids into Hepa1-6 mouse hepatocarcinoma cells. As expected, luciferase activity from cells transfected with the positive control vector pMIR-122+ was strongly reduced upon miR-122 overexpression (7- to 10-fold, Figure Figure5).5). Conversely, pMIR-122––transfected cells were unaffected by miR-122 overexpression (Figure (Figure5),5), which suggests that miR-122 overexpression is efficient and specific. Likewise, transfection of the miR-122 mimic significantly reduced luciferase counts from the positive control pMIR-mAldoa (2.23-fold; P = 0.003), whereas luciferase counts from pMIR-mAldoa mut, which contains mutated miR-122 seed sequences (Supplemental Table 3), were unaffected. Importantly, overexpression of the miR-122 mimic significantly reduced expression from pMIR-mHfe (2.72-fold; P = 0.00002) and pMIR-mHjv (1.74-fold; P = 0.0001), but not from constructs in which the predicted miR-122 binding sites are mutated (pMIR-mHfe mut and pMIR-mHjv mut) or from pMIR-mGapdh, which does not contain a predicted miR-122 target site (Figure (Figure5).5). The suppressive effect of miR-122 on pMIR-mHfe and pMIR-mHjv was maintained for at least 72 hours after transfection (Supplemental Figure 5). We further observed a minor reduction of luciferase counts from pMIR-mHamp upon miR-122 overexpression. However, this effect appears not to be specific, because a similar level of reduction was obtained when the predicted miR-122 target site was mutated. Taken together, these data show that the 3′-UTRs of Hfe and Hjv are direct targets of miR-122.
To investigate how hepatic miR-122 inhibition leads to elevated spleen iron levels (Table (Table2),2), we analyzed mRNA expression of iron-related genes on a specialized microarray platform (IronChip; mouse version 9). Interestingly, we observed increased mRNA expression of Tfr1 and several enzymes involved in heme biosynthesis — hydroxymethylbilane synthase (Hmbs), ferrochelatase (Fech), uroporphyrinogen decarboxylase (Urod), coproporphyrinogen oxidase (Cpox), and delta-aminolevulinate synthase 2 (Alas2) — as well as the hemoglobin α adult chain 1 (Hba-a1) in the spleen of PM_anti–miR-122–injected mice at 3, but not 6, weeks after treatment (Figure (Figure6A).6A). Increased mRNA expression of Tfr1, Hmbs, Alas2, and Hba-a1 as confirmed by qPCR analysis (Figure (Figure6B).6B). Additionally, miChip analysis of splenic total RNA indicated that 3 miRNAs (miR-451, miR-17, and miR-29b) were selectively increased in the spleen of PM_anti–miR-122–injected mice (Supplemental Figure 8), a result confirmed by miRNA qPCR (Figure (Figure6C).6C). miR-451 was previously linked to murine and human erythroid differentiation (53–55), in that its transcription is activated by the erythroid transcription factor Gata-binding factor–1 (Gata1), which drives erythroid differentiation to a late stage (53–55). miR-451 in turn suppresses Gata2, which is responsible for maintaining the erythroid precursor stage. Consistent with enhanced erythropoiesis in the spleen of PM_anti–miR-122–injected mice, we observed increased miR-451 and Gata1. Gata2 mRNA levels only showed lower expression in PM_anti–miR-122–injected mice compared with the saline control (Figure (Figure6D).6D). Collectively, these data suggest that inhibition of miR-122 expression leads to iron deficiency, which in turn increases extramedullary hematopoiesis.
Hepatocytes sense systemic iron availability and regulate systemic iron fluxes. Plasma iron levels are controlled by 2 major hepatic signaling pathways: the HH-associated proteins (Hfe, Hjv, and Tfr2) and the Bmp/Smad signaling pathway, which control the hepcidin response to systemic iron availability, and proinflammatory cytokines and the Jak/Stat pathway, which mount the inflammatory response of hepcidin (56, 57). Activation of either of these signaling pathways increases hepcidin levels, which leads to decreased duodenal iron absorption and iron release from macrophages. In contrast, TMPRSS6 (58) and the inhibitory Smad protein Smad7 (59) suppress hepcidin levels.
In this study, we showed for the first time to our knowledge that systemic iron homeostasis is also controlled by a miRNA. Hepatic miR-122 expression was critical to prevent iron deficiency in plasma and liver, impairment of hematopoiesis caused by low iron availability, and extramedullary erythropoiesis in the spleen. Thus, decreased miR-122 expression, which was observed in Hfe–/– mice and HH patients (Figure (Figure1,1, A and D), may be a compensatory response of the liver to limit iron uptake and counteract liver iron overload. However, the biological significance of the decreased miR-122 levels in Hfe–/– mice needs to be established, because hepatic miR-122 reduction in Hfe–/– mice failed to increase mRNA expression of the known miR-122 target gene Aldoa or to affect molecular pathways that reduce cholesterol levels in miR-122–depleted mice (data not shown). Thus, it may simply be a marker rather than a functionally consequential response.
Our findings demonstrated that hepatic miR-122 inhibition by LNA-modified PM_anti–miR-122 was highly specific: miR-122, and no other miRNA, was exclusively inhibited in the liver upon PM_anti–miR-122 injection (Supplemental Figure 2), and 2 mismatches within the LNA-modified anti–miR (2MM_anti–miR-122) were sufficient to prevent hepatic miR-122 inhibition and alterations in systemic iron homeostasis (Figure (Figure22 and Table Table1).1). Interestingly, hepatic miR-122 inhibition in the liver of WT mice and in primary murine hepatocytes caused increased mRNA expression of Hamp and 3 of its transcriptional activators (Hfe, Hjv, and Bmpr1a; Figures Figures33 and and4).4). Notably, increased Hfe, Hjv, and Bmpr1a mRNA expression was also reported in 3 previous studies on miR-122 antagonism in mice (31, 32, 60). This suggests that the observed stimulation of Hfe and Hjv mRNA expression is specific for miR-122 depletion and independent of the antisense chemistry applied. Elevated expression of Hfe, Hjv, and Bmpr1a would be expected to stimulate the Bmp/Smad signaling pathway in mice (44). However, increased Smad1/5/8 phosphorylation in protein extracts from PM_anti–miR-122–injected mice was not detected (Supplemental Figure 6). Consistently, mRNA expression of Id1, a target gene of the Bmp/Smad signaling pathway (48), remained unchanged in the liver of PM_anti–miR-122–injected mice (Supplemental Figure 3). High hepcidin levels would be expected to cause iron retention (e.g., in splenic macrophages) and decreased iron absorption by stimulating the internalization and degradation of the iron exporter ferroportin (4). However, ferroportin protein levels in the spleen were not decreased in PM_anti–miR-122–injected mice (Supplemental Figure 7). We speculate that the iron demand for extramedullary erythropoiesis that occurs in the spleen of PM_anti–miR-122–injected mice needs to be satisfied, and thus hepcidin-independent regulatory mechanisms, such as BACH1- or NRF2-mediated transcriptional control of ferroportin (61), may override the hepcidin response of ferroportin.
Extramedullary hematopoiesis in PM_anti–miR-122–injected mice is characterized by increased splenic mRNA expression of Tfr1, enzymes involved in heme biosynthesis (Hmbs, Fech, Urod, Cpox, and Alas2), and Hba-a1 as well as the hematopoietic miR-451. Interestingly, overexpression of Hba-a1 mRNA in anemic mice correlates with rapid recovery from anemia (62), which suggests that similar mechanisms may apply to PM_anti–miR-122–injected mice. Also of note is the elevated expression of miR-451, which was previously reported to be essential for erythropoiesis in mammals and zebrafish and shown to depend on Gata1 (53–55). Elevated miR-451 expression is responsible for decreased expression of Gata2, a transcription factor required to maintain the erythroid precursor stage. Here we show that Gata1 and miR-451 expression increased and Gata2 mRNA levels tended to decrease in the spleen of PM_anti–miR-122–injected mice, which suggests that this regulatory network is maintained during murine extramedullary erythropoiesis in the spleen. It is intriguing to speculate that increased expression of miR-29b and miR-17 (Figure (Figure6C)6C) — which was also observed in miR-122–depleted mice and, to our knowledge, previously not linked to erythropoiesis — may also be relevant for this process. Taken together, our findings suggest that iron deficiency induced by miR-122 limitation triggers erythropoiesis in the spleen to avoid anemia.
We further uncovered a mechanistic link among miR-122 depletion, elevated hepcidin expression, and systemic iron deficiency. We showed that Hfe and Hjv, 2 genes mutated in the frequent iron overload disorder HH, contained functional miR-122 binding sites (Figure (Figure5,5, Supplemental Figure 5, and Supplemental Table 3). Importantly, mutation of the predicted miR-122 binding sites prevented the suppressive effect of miR-122, which suggests that miR-122–dependent inhibition of Hfe and Hjv is specific.
The fatty acid and iron metabolic networks are interconnected; however, the identification of common regulatory elements has been elusive. Recently, several studies showed that miR-122 is required to maintain serum cholesterol levels in mice and nonhuman primates (30–32, 63, 64). However, the miR-122 target gene responsible for this phenotype has not been discovered. In addition to its role in maintaining iron and cholesterol metabolism, miR-122 is critical for efficient HCV replication (33–35, 65). Inhibition of hepatic miR-122 in chimpanzees chronically infected with HCV led to long-lasting suppression of both HCV replication and viremia (64). miR-122 expression is also reduced in hepatocellular carcinoma (28, 29) and is regulated by the circadian rhythm (60), which suggests that miR-122 activity may be required to synchronize genes within overlapping metabolic pathways. In recognition of the circadian rhythmicity of miR-122 expression, samples from the mice analyzed in this study were always collected at the same time of the day and sacrificed in a random order. Furthermore, the link between miR-122 expression and important human diseases suggests that miR-122 could be a target for therapeutic intervention.
In summary, we propose the following model for the role of miR-122 in iron homeostasis: miR-122 regulates the expression of the HH-associated proteins Hfe, Hjv, and possibly others. Overexpression of Hfe and Hjv activates Hamp mRNA expression (66). Elevated hepcidin levels will limit the iron export capacity from duodenal enterocytes and macrophages and cause plasma and liver iron deficiency. As a consequence, iron-deficient hematopoiesis develops, and extramedullary hematopoiesis is observed in the spleen. Future experiments are needed to clarify the signaling pathways that control the Hamp response to elevated Hfe and Hjv expression in anti–miR-122–treated mice.
All mice were housed in the European Molecular Biology Laboratory animal facility under a constant light-dark cycle, maintained on a standard mouse diet, and allowed ad libitum access to food and water. Mice were euthanized by CO2 inhalation. All mouse breeding and animal experiments were approved by and conducted in compliance with the guidelines of the European Molecular Biology Laboratory Institutional Animal Care and Use Committee. A total of 48 female WT mice on the C57BL/6J genetic background were injected at 10–12 weeks of age. 16 mice were injected i.p. with 25 mg/kg PM_anti–miR-122 (31) in isotonic solution (0.9% NaCl), with perfect complementarity to miR-122. As controls, 16 mice were injected i.p. with 400 μl NaCl or with 25 mg/kg 2MM_anti–miR-122 (designed with 2 mismatches toward the miR-122 seed sequence; ref. 31) in isotonic solution. Hfe–/– mice were described previously (36). Induction of iron overload by a dietary regimen (67) or iron-dextran injection (8) has been described previously.
Heparinized blood was collected by cardiac puncture. Hematological parameters were determined using a blood counter instrument. Plasma iron content was assayed using the Iron Kit (Thermo Electron GmbH) in a 96-well format using a serial dilution of iron atomic absorption standard solution (1,000 mg/ml iron in HCl; Sigma-Aldrich). The unsaturated iron binding capacity was measured using the U.I.B.C. kit (Biolabo) according to the manufacturer's instructions. Tissues isolated from anti-miR–treated mice were analyzed for non-heme iron as previously described (68, 69). Total plasma cholesterol was analyzed using ABX Pentra Cholesterol CP (Horiba Group, Horiba ABX Diagnostics) according to the manufacturer's instructions. The measurements were carried out in duplicate and were correlated to a 2-fold diluted standard curve generated from an ABX Pentra MultiCal solution (Horiba ABX Diagnostics).
Tissue was disrupted using a Tissue Lyzer (Qiagen), and total RNA was isolated using TRIzol (Invitrogen). RNA quality and concentration was assessed using a 2100 Bioanalyzer (Agilent Technologies) and Nanodrop ND-1000 (Nanodrop Technologies), respectively. For mRNA analysis by qPCR, 2 μg total RNA was reverse transcribed with random hexamers using Superscript II Reverse Transcriptase (Invitrogen) following the manufacturer's instructions. qPCR was carried out using 10 ng cDNA in a 20-μl reaction volumes using SYBR Green I (Applied Biosystems) on a Prism 7500 (Applied Biosystems) and gene-specific primers. Relative qPCR expression of individual genes was normalized to the expression of reference genes Gapdh or actin B (Actb) using qBASE (70) and the ΔΔCq method (71). See Supplemental Table 4 for primer sequences.
Pseudonymized male human tissue samples were provided by the Tissue Bank of the National Center for Tumor Diseases Heidelberg (project no. 564) after approval by the ethics committee (no. 206/2005, Medical Faculty, Heidelberg, Germany). Because only long-term archived (>5 years), pseudonymized formalin-fixed, paraffin-embedded (FFPE) tissues were used, informed consent was not required for this study. 3 7-μm-thick FFPE sections were dewaxed in 500 μl xylene by incubation at 65°C (3 g) for 3 5-minute periods. The dewaxed sections were washed 3 times in 500 μl 100% ethanol (2 minutes; 15,700 g). After discarding the supernatant, samples were dried for 1 hour at 37°C. Tissue was digested using 10 μl proteinase K in 150 μl PKD buffer (Rneasy Kit FFPE, Qiagen) for 15 minutes at 55°C, followed by proteinase inactivation for 15 minutes at 80°C. Total RNA was extracted using the miRneasy kit (Qiagen).
For IronChip analyses, 5 μg total RNA was reverse transcribed, amplified, and labeled as described previously (72). Expression of miR-122 in the anti-miR–injected mice was analyzed by miRNA-specific TaqMan assays according to the manufacturer's instructions (Applied Biosystems).
For miRNA expression profiling by miChip, we pooled equal amounts of total RNA extracted from each mouse within the individual groups and analyzed RNA as previously described (37–39). Array data were deposited in the Gene Expression Omnibus repository (GEO; accession no. GSE24786; ref. 73). Hepatic miR-122 levels in Hfe–/– mice were analyzed by miRCURY-LNA specific miRNA assays according to the manufacturer's instructions (Exiqon). miRNA measurements in the spleen (miR-451, miR-29b, and miR-17) and liver of anti–miR-122–injected mice, in patient biopsies (miR-122 and RNU6), and in primary hepatocytes (miR-122 and RNU6) were analyzed by miRNA qPCR (see Supplemental Methods). Independent of the method of qPCR analysis, relative miRNA expression levels were calculated using qBase (70) and determined relative to the appropriate reference gene(s).
cDNA for the 3′–rapid amplification of cDNA ends (3′-RACE) reaction was synthesized as described above, except that reverse transcription was primed using an oligo(dT)-adapter primer (Qiagen). 3′-RACE was carried out in a final volume of 50 μl containing 1 pmol of gene-specific primers (Supplemental Table 3), 20 ng cDNA, 25 mM dNTP mix, and 2.5 U Thermo-Start Taq polymerase (Thermo-Fisher). Amplification was performed with an initial activation step at 95°C for 15 minutes, followed by 40 cycles of 95°C for 30 seconds, 60°C for 30 seconds, and 72°C for 3 minutes. Primers were designed to amplify the full-length 3′-UTRs of mouse Hamp (bp 1–bp 106), Hjv (bp 1–bp 519), Aldoa (bp 1–bp 183), Gapdh (bp 1–bp 178), and Hfe (bp 1–bp 545). Predicted miR-122 binding sites are shown in Supplemental Table 3.
pMIR is a derivative of the pGL3 vector (Promega) that was engineered to accommodate a multiple cloning site 3′ of the firefly luciferase gene. PCR products were cloned into the SacI/NheI sites and sequenced. To generate a positive (pMIR-122+) and a negative (pMIR-122–) control vector, a double-stranded DNA oligo nucleotide with the identical sequence of miR-122 (see Supplemental Table 3) was inserted in the pMIR-SacI/NheI cloning sites either in the sense (+) or antisense (–) orientation.
Site-directed mutagenesis of the predicted miR-122 seed sequence (see Supplemental Table 3) within the 3′-UTR of the genes of interest was performed using GeneTailor System (Invitrogen) following the manufacturer's protocol.
Murine primary hepatocytes were isolated according to an optimized protocol described previously (ref. 74 and Supplemental Methods) and transiently transfected in 6-well plates using RNAiMAX (Invitrogen) with PM_anti–miR-122 (500 ng; Santaris Pharma) or a scrambled oligo control sequence (500 ng; Santaris Pharma). Total RNA was extracted 24 and 48 hours after transfection using TRIzol (Invitrogen).
For miR-122 overexpression, Hepa1-6 cells were transiently transfected in 24-well plates using Lipofectamine 2000 (Invitrogen) and 5 μM pre–miR-122 (Ambion) as well as 0.05 ng renilla and 10 ng firefly luciferase vectors. Cells were lysed at the indicated time points using 100 μl of 1× passive lysis buffer (Promega). To assay luciferase counts, we used a microplate luminometer (Microlumat plus LB96V; Berthold), 25 μl cell lysate, and 10 μl of both LAR II (Promega) and Stop and Glo (Promega).
Data are expressed as mean ± SD. Results compared between groups were analyzed by 2-tailed Student's t test when 2 samples were considered or by 1-way ANOVA for 3 or more samples. When groups were compared using 1-way ANOVA, we assumed that data were normally distributed. A P value less than 0.05 was considered significant.
We thank Bruno Galy for his excellent advice, Katarzyna Mleczko for sharing her data, Sandra Manthey for helping with the primary hepatocyte preparation, and Steven Dooley and Rolf Gebhardt for sharing their knowledge of RNAi knockdown in primary hepatocytes. We thank the Tissue bank of the National Center for Tumor Diseases Heidelberg for providing the biopsies of hemochromatosis patients. The authors gratefully acknowledge John D. Ryan for critical reading of the manuscript. M. Castoldi is supported by an Excellence Fellowship of The Medical Faculty of the University of Heidelberg. This work was supported by a NIH grant to R.E. Fleming (R01 DK 063016); a Network HepatoSys/Virtual Liver grant (BMBF) to M.U. Muckenthaler, L.A. D'Alessandro, and U. Klingmüller; and a DFG grant to M.U. Muckenthaler and M. Castoldi.
Conflict of interest: J. Elmén, M. Lindow, and S. Kauppinen are employed by Santaris Pharma, a biopharmaceutical company engaged in the development of RNA-based medicines.
Citation for this article: J Clin Invest. 2011;121(4):1386–1396. doi:10.1172/JCI44883.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Alizee Mills
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Listing information
- Beds: 4
- Stories: 0
- Foreclosure: No
- Lot Area: 0.92
- County: Kalkaska
- Style: Ranch
- Baths: 3 (Full: 1 3/4: 1 1/2: 1 Other: 0)
- Parking Type Description: Attached 2 cars
- Total Square Footage: 2,541 ($364.03 per sq. foot)
- Lot Size: 200 X 200
- School District: Kalkaska Public Schools
- Year Built: 1947
- Status: Active
- New Construction: No
- Above Ground Sq. Feet: 2,541
- Estimated Annual Taxes: $12,366
- Body of Water: Torch Lake
ADDITIONAL BUILDINGS Guest House, Other, Secondary Garage(s)
APPLIANCES/EQUIPMENT Dishwasher, Dryer, Microwave, Oven/Range, Refrigerator, Washer
ASSOCIATION FEE INCLUDES None
Bedroom 1 Floor Covering Wood
BR2 Floor Covering Wood
BR3 Floor Covering Wood
BR4 Floor Covering Wood
CONSTRUCTION Frame
Deeded Waterfront Yes
DEVELOPMENT AMENITIES None
DOCUMENTS ON FILE LBP Disclosure, Other, Sellers Disclosure
DRIVEWAY Concrete
ECO Features No
EXTERIOR FEATURES Deck, Other, Patio, Porch, Screened Porch
EXTERIOR FINISH Stone, Wood
FIREPLACES AND STOVES Fireplace(s), Wood
FOUNDATION Block, Full
HEATING/COOLING SOURCES Natural Gas
HEATING/COOLING TYPES Forced Air
INTERIOR FEATURES Built-In Bookcase, Island Kitchen, Solarium/Sun Room, Walk-In Closet(s)
Kit Floor Covering Vinyl
LAND FEATURES Level
Liv Rm Floor Covering Wood
Master Bath Private
Other # Baths: Lower - 0, # Baths: Main - 3, # Baths: Upper - 0, Above Gr. FINISHED SF Apx - 2541, Addl Building Dimensions - 2 Guest Houses, Below Gr. UNFIN. SF Apx - 864, Development Name - Crystal Beach, Garage Dimensions - 24 X 22, Summer Taxes - 8316.75, TOTAL Baths - 2.5, Water Front Footage - 134, Winter Taxes - 4049.30, Year Remodeled - 2005, Year: Summer - 2015, Year: Winter - 2015
POSSESSION Negotiable
PRIMARY GARAGE Attached
Principal Residence Yes
Private/Shared Private
ROAD Blacktop, Public Maintained
ROOF Asphalt
SEWER Private Septic
Sign (Y/N) Yes
TV SERVICE/INTERNET AVAIL Cable TV
UNIVS DESIGN/BARRIER FREE None
WATER Private Well
WATER FEATURES All Sports, Inland Lake, Priv Frontage (Wtr Side), Sandy Bottom, Sandy Shoreline
ZONING/USE/RESTRICTIONS Other, Residential
Bedroom 1 Main Floor 18.3x14
Bedroom 2 Main Floor 13.11x14.8
Bedroom 3 Main Floor 12.4x15.1
Bedroom 4 Main Floor 10.4x15
Kitchen Main Floor 14x20.3
Laundry Room Main Floor 12.3x14.8
Living Room Main Floor 18.3x30.8
Listing Courtesy of Donald Fedrigon of Re/Max of Elk Rapids PO Box 36 Elk Rapids Mi 49629


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Flowering Time ~56 days
Dominance mostly indica
Bio While its two parts could be viewed bigger than its whole it is a strain that is a must grow at some stage for all level of growers. Northern lights made it into just about everyone's grow room in some form at some time over the passed 30 years as too did Skunk so it would seem poetic justice to give this strain a place in any grower`s room who is interested in the predominant indica side of things.Heritage: NL5 x SkunkCategory: The Naturals - Old school geneticsFamily of breed: 50% Afghan 50 % SkunkBreeder: Nevil and ShantibabaPreferred medium: Bio and Hydro Indoors and outdoors/greenhouseExpected yield: With both the NL5 and Skunk being prolific in yield this is a strain that can be very favorable in quantity harvested. It would be expected to get 600 g per m2 as a minimum.Flowering period: Indoor flowering usually takes between 7-9 weeks depending upon how the plant is grown. Outdoors we expect it to be completed in October(mid) Northern Hemisphere or March/April(southern Hemisphere).Recommendations: For the inexperienced growers to the most advanced. Especially for greenhouse growers worldwide or short outdoor seasons.Special Notes: Combining two such well proven strains in Cannabis history it is hard to fault this strain. Its pedigree stands the test of time.
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do you think you would be able to memorize all your lines, paragraphs, Pages? I know when your very passionate about something you can make it work but I think a lot of people have problems memorizing especially if it's too much. I know practise makes perfect. It's also extremely competitive. There's good actors out there that don't get jobs sometimes. At the moment I'm doing sales associate as a job part time. This job is very important to me because I had no luck for one year and before that several years 😕. Extremely difficult to find work but I am planning to practise monologues for acting, and make sure I feel confident enough to post it on YouTube. this way I still would enjoy doing it even if nothing happens. People post videos and try but not everyone will get famous. Would you be able to memorize a lot? Thanks to everyone who answers.
- YesVote A
- NoVote B
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The daughter of a former co worker graduated from acting school a few years ago and to this day i think she still works as a waiter.
good luck with that.11|00|0
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I have the memory of a gold fish so yes...00|00|0


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It's no wonder that so many investors wind up confused by their portfolio performance.
Consider "value" funds, for instance. You would think that all mutual funds that promise to invest according to a value philosophy should post very similar returns. But that's not the case.
Inspect the three- and five-year returns on any mutual fund database and you'll see value funds with outstanding results and value funds that lag behind the market.
How can value stocks both underperform and outperform at the same time? It comes down to how one views value. There are factors that make some value stocks more appealing than others and play a crucial role in determining their performance.
To test what truly differentiates one value stock from another, we examined the historic performance of two value portfolios: one that simply owned cheap stocks; another that favoured owning cheap stocks with some additional characteristics.
We assumed that both portfolios selected stocks with low price-to-earnings ratios and low price-to-book ratios, two common measures of value. The difference between the two portfolios was that the manager of Value Fund A focused simply on buying the least expensive stocks, while the manager of Value Fund B preferred to own the least expensive stocks with what we would define as "good earnings," as identified by three key characteristics:
Their earnings have grown;
They have generated better-than-expected earnings;
Analysts have raised their estimates of future earnings for the stock.
Using CPMS/Morningstar's back-testing program and real-time database, we selected each 40-stock portfolio from the S&P/TSX Composite Index. At the end of every month, we removed stocks that no longer fit the criteria. We replaced any stock we removed with the stock that ranked highest on our criteria. Each test was conducted using monthly data from December 1985 to December 2012.
So what impact does good earnings have on value portfolios?
Value Fund A (the one that simply bought cheap stocks) had an annualized return of 5.0 per cent over the 27-year test period, significantly underperforming the benchmark S&P/TSX Composite Index's return of 8.2 per cent over the same period.
Value Fund B (with earnings growth as part of the investment strategy) performed much better, generating an annualized return of 11.6 per cent over the past 27 years, easily outperforming the index.
In addition, Value Fund B outperformed Value Fund A in 20 of the 27 years tested. The performance of Value Fund B was also more consistent, generating positive returns in 21 out of 27 years versus a more modest 17 out of 27 for Value Fund A.
The test results clearly show that good earnings make a significant difference in the long-term performance of value stocks, helping to generate more consistent and superior returns.
It is clear that not all value funds are alike. Those that incorporate good earnings in their strategies are more likely to outperform.
So investors may wonder how they can be certain that they are buying a value fund that incorporates earnings growth in its decision-making strategy.
The answer is simple: Ask.
Ask the fund, or your financial adviser, to show you proof of the fund's current style and historic style. A good fund manager will be able to supply proof of their underlying approach to stock selection.
David Fruitman, CFA, is Vice President & Portfolio Manager; Brennan Carson, CFA, is Vice President of Client Service and Business Development at STYLUS Asset Management Inc.Report Typo/Error
Follow us on Twitter: @GlobeInvestor


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Professional Learning Specialists
Traci Lenihan
Depth and flexibility optimizes Tracie's expertise. Having been an educator for over 16 years, her experience is derived from classroom teaching, working as a Technology Specialist at both the school and district level, leading teachers and administrators as the Professional Development Coordinator for the New Mexico Public Education Department's Virtual Schools bureau, and currently as the Educational/Instructional Technology Facilitator for Spokane Public Schools. Tracie has extensive experience in designing online, self-paced professional development courses and offers them through both online and blended opportunities in order to meet the needs of the educators she supports. Tracie earned her Bachelor degree from California State University and a Masters of Education in Curriculum and Instruction at New Mexico Highlands University. No matter the position, Tracie brings a blended, interactive approach in meeting student, teacher, and administrator needs.
Being a Microsoft Innovative Educator trainer has given her the opportunity to share with and learn from fellow educators across the country. In her spare time, she enjoys exploring the Pacific Northwest and spending time with her husband Patrick and their son, Rielly.


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everal meta humans are being killed. And it seems they were killed by their abilities.
Mia Sara Harley Quinn
Ian Abercrombie
Shemar Moore Jesse Reese
Rachel Skarsten
Dina Meyer Barbara Gordon
Ashley Scott Helena Kyle
Chris Long Director
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This online memorial was created in loving memory of Anne Moringello, whose life story is told throughout this memorial website.
Please sign Anne's guest book and let us know you came to visit. We will remember Anne forever.
Anne was born in 1937 to Anne and Henry in Sussex, England. Only a few months later, her father Henry died from complications from World War I injuries, and in another couple of years she and her mother moved to Melbourne, Australia, where her mother's sister, Aunt Mary, and other relatives had relocated.
Anne's childhood was filled with friends and cousins, most of whom still live right there in Melbourne. At 18 Anne embarked on a Grand Tour, staying in various places in Europe and making lifelong friends.
After this colorful young-adulhood, Anne settled back in Melbourne with her mother. In 1973 she started corresponding with a gentleman she "met" through a travelers' club -- Sal Moringello. A year later Anne visited him in his hometown of New York City, and a year after that, she moved there permanently and they were married. Soon, her mother moved to New York as well, and the newlyweds lived in an apartment with both of their mothers. Anne and Sal had bonded over common interests, situations, and goals, including a love of travel, being "older" (mid-30s) only children who had never married, and living with and being devoted to their elderly widow mothers.
In 1977, Anne's own dream of motherhood came true when she gave birth to Sally-Anne, a daughter very much wanted and welcomed by both Anne and Sal. Unfortunately, Sal's mother had passed away before her granddaughter was born (but died knowing a grandchild was on the way). Anne's mother passed away when Sally-Anne was four.
Anne made four trips back to Australia after moving to America -- in 1982, 1999, 2001, and 2006. Sally-Anne joined her on the first and last of these. Both mother and daughter, as well as the Australian relatives and friends, were thankful for the opportunity for the last visit, as Anne went into the hospital only two weeks after returning from Australia, and never went home again.
Anne died on July 6, 2006, at Mt. Sinai Hospital of Queens after a battle with Non-Hodgkins Lymphoma.
Please sign the guestbook for Anne by clicking here
This page has been visited 20765 times


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Apple are promising any iPad 3 owner who bought their model in the last 30 days a completely free upgrade to the new iPad 4. This news is surprising, but not quite as surprising as what appears to be a complete purge of the iPad 3 from the Apple store online. Whether they will still be stocking them in their high street stores is unclear as of yet, and although they are still available from third-party stores and sellers the total absence of them from their website is quite a strong indication that they have been removed for good from Apple's shelves.
Note for New iPad 3 Users : If you bought your iPad in the last 30 days , get a replacement from Apple for the new iPad 4 . More details here .
All comparisons between of the iPad 4 are being made against the new mini version and the iPad 2 and is. This lends further wonder to the question of why the iPad 3 has been wiped from Apple's website like a smear from a window. The problems with over-heating were well-known as were several other glitches with the product, but did they really warrant Apple's denial of existence?
But enough psychoanalysing Apple's motives and let's move on to what is now the main comparison between the iPads; what's new on the iPad 4 and how does it match up to the iPad 2?
Just having a quick scroll down on Apple's iPad comparison page shows the viewer quite clearly that the iPad 4 is the superior model when compared to its older sibling, the iPad 2.
Storage and Price
The very first section is quite telling as the iPad 2 is only available for £329 (WiFi edition) or £429 (3G edition) in 16GB.
The iPad 4 is available in 16 GB – 64GB in both WiFi and Cellular models at prices ranging from £399 – £659.
With the inclusion of Retina display, the new A6x chip and the full HD video recording the price jump of £70 for a 16 GB WiFi model iPad 4 is not too steep when you consider what extras you are getting for your money.
The iPad 4 is only a tiny bit deeper and heavier than the iPad 2 weighing in at 1.44 pounds compared to the iPad 2's 1.33 pounds (WiFi editions).
The depth is increased by .03 of an inch with the iPad 4 measuring 0.37-inches and the iPad 2 coming up at 0.34-inches.
This is such a minuscule difference that is would ever be the deciding factor between the two.
This is one of the biggest differences between the two models and the Retina display on the iPad 4 promises to deliver stunning imagery. This will certainly be the tipping factor for many people as the screen you are using is of vital importance and is the interactive part of the screen – people will want it to be a beautiful as possible.
Another huge factor in the iPad 4's favour is the inclusion of the new A6x chip, which is offering twice the speed of the old A5 chip. People looking to use the iPad for personal and casual use might be less likely to be swayed by the new chip and may prefer the smaller price tag of the iPad 2, although anyone buying an iPad for heavy or professional use might prefer the updated graphics of the A6x and the knowledge that they have the highest processing power currently available on an iPad.
Camera and Video
A reason to choose the iPad 4, and coupled with the Retina display, which makes the new model ideal for photographers, is the improved camera and video recording on the iPad 4. With the iPad 2 offering VGA photos from the front camera and 920 x 720p from the back camera, it has no chance of competing with the iPad 4's 5MP iSight camera and 1.2 MP FaceTime camera.
An obscure, but relevant selling point here is if the iPad is being used by travellers then the quality of the picture being sent and received over FaceTime to communicate with friends and loved ones back home will actually be very important.
Video capture on the iPad 2 is 720p HD recording while the iPad 4 has 1080 full HD recording capability.
Another huge selling point for the iPad 4 is bound to be the LTE technology. LTE offers huge speed increases against the old 3G of the iPad 2, and is guaranteed to pull more and more customers especially as more networks start offering the service.
This will be the iPad 2's main lure. All those docks and adapters you have at home? Yeah, they are not going to work with the sparkly new iPad 4. The iPad 2, however.
Both iPads offer the same battery life of up to 10 hours WiFi web surfing or 9 hours 3G/cellular internet usage.
Artificial Intelligence
iPad 4 has Siri, iPad 2 does not.
So hopefully, this has given you an overview of what is new on the iPad 4 compared to its main full-sized rival the iPad 2. Would you consider going back to the iPad 2, or is the iPad 4 too much of an attractive lure ?


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Never Felt So Lovely / Azalea
It's Gonna Rain/Our Simple Little Song
Eyes Open (Single)
Lost Kids
High Hopes (EP)
Is It Too Much To Ask?
American Songbook Vol. 3
Now Or Never EP
Strange Train EP
The Country Is Behind Us EP
American Songbook Vol. 2
American Songbook Vol. 1
Jackson Summer Nights
Body & Mind
Ocean of Blood
Love Him, But Don't Try To Take Him Home
Nachtmuzik (EP)
Spiral Stairs Vol. II
Spiral Stairs Vol. I
J.T. Makoviecki Jackson, New Jersey
I've been making music since I could sing to James Brown and Phil Collins as a 3 year old. Ever since I've recorded however
possible: tape deck, 8track, MacBook, etc. I use raw recording techniques and low-fi equipment to make music with my friends.
enjoy. ... more
Streaming and Download help


text:
I've been arranging a sale of some of my nudism catalogue and so have been spending some time flicking through them again. Some of the books come from the early days, from the 1930s, the inter-war years of experimentation, particularly on continental Europe where nudism seemed to really take off. I have to say, the more I read, the more I just don't get it. Yes, I can see there's a certain frisson and freedom involved in skinny-dipping at the beach or swimming-hole, I can even appreciate the thrill of wandering around outdoors naked and feeling the air on bare skin (weather and legality permitting) but reading these older nudism books one is left with the feeling that this 'movement' must have been in one of the greatest communal exercises in denial of all time. Fortunately, I have a number of more recent publications too which tell me that these days nudism has kind of caught up on itself and allows for both recreational and sexual naked freedom in many of its venues. This is all very well, and I have nothing against you if this is your cake, but I think I'll pass.
Still, these photos above (and boy did I have to hunt to find male nudes in these books) struck me as having a certain elegance and if those old-time nudists knew how to do anything well, it was how to strike a pose in front of the camera. Of course there is actually a whole body of nude photography which these photos are a part of, the Frei Korper Kultur movement in Germany at about this time spawned a huge number of photographs of athletic young men and women leaping, bounding and otherwise being athletic and there is always a section of this kind of work in any Taschen anthology of nude photography or treatise on 'The Naked Male in History'. I suppose its true to say that even Leni Reifenstahl's photos of the 1936 Olympians are part of that same tradition.


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Please confirm that you want to add Honor Your Family By Making Your Own Family History Videos to your Wishlist.
Some people are fortunate enough be able to commission beautiful video biographies of family members, to be handed down and cherished for generations. But, the process is long and expensive. Not everyone can afford a video biography done by a professional.
The typical Family Legacy Video is a very expensive investment, running into the thousands of dollars.
The good news is that, even Hopelessly Busy People Can Make a Family Legacy Video in a Few Weekends and For Very Little Cost.
It always breaks my heart when I see families with elders who have wonderful, important stories to tell, but they never get recorded or preserved because the family either can't afford to have it done, or they just don't know what to do.
That's why I decided to develop this course: to show you how to do it yourself, and to do it quickly at very little cost.
I lead you through the process step-by-step. I'll take you from the initial idea, to getting your family member to sit in front of your camera, setting up your video shoot, getting good quality video, sound, and lighting, editing, and finally, turning the project into a polished DVD as a gift for the entire family, both now and for generations to come.
There are many reasons to create a Family Legacy Video:
A Family History Video may be your most important legacy. In this course, you will learn how to make one relatively quickly, and at a small fraction of the cost of hiring a professional.
Contents and Overview
In over 2 hours of content, including 22 lectures, this course covers why Family Legacies are important, how to choose a subject, convincing your subject to tell their story, collecting the photos and memorabilia and organizing it.
This course also covers what equipment you'll need, how to set it up for the video shoot, sound and lighting, and what kind of questions to ask your subject on camera.
You will also learn about editing your video with photos, archival footage, and music, among other important lessons.
Upon completion students will have the knowledge and skills to conceive of a biographical video documentary, featuring an important loved one in the family, set up an effective video recording session, in which the subject tells the stories from their past, including memories, experiences, wisdom and advice for future generations. They will be able to take the raw video, import it into a video editing software program, and turn it into a compelling video biography on DVD for showing the family, and for preserving for 100 years and beyond.
In this lecture we explore the reasons that it is so important that we capture and preserve the stories and experiences of our family members, or even our own stories in order to hand them down and make them accessible to future generations.
In this lecture we look at what makes a Family History Video; what i is, and what is its purpose.
Even though we are well-intentioned, most of us put off recording our stories and experiences. This lecture looks at the reasons, and how to overcome our blocks and objections.
We take a look at deciding who will be the subject of our first Family History Video.
This lecture examines the ways in which we can accomplish the sometimes difficult task of getting our potential video subjects to agree to participate in our Family History Video project.
A discussion of the various elements that can be included in your Family History Video, including topics of discussion.
Stan has spent the majority of his life creating music. He began his piano study at age 5, and studied all forms of keyboard music over the years, both privately and in college. He began playing piano professionally at the age of 19, over 40 years ago. Through the years, he has appeared in countless lounges, night clubs, cabarets and showrooms all over the United States. He has recorded at studios in New York, Nashville and Los Angeles. Recently, he finished up an 8-year stint as a Piano Bar entertainer at the prestigious Eldorado Hotel in Reno, Nevada. A full time performer and published songwriter, Stan has vast experience in the music industry.
He also runs a Video Production Business, specializing in Family History Legacies and Personal Biographies.
Stan is experienced in the following areas:
- Night club, cabaret, lounge, and concert entertainer
- Recording artist
- Music Producer
- Music Teacher
- Video Production
- Genealogy


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Photos are for members only!!
Peachmate is a 100% free sex site for adults looking for fun with no strings attached.
Escort ads, casual sex groups, personal ads, video chat and much more.
Athleticaly built ex forces i am genuine honest and always up for a laugh but am abit of an animal in the bedroom!!


text:
|Scarborough JE, Schumacher J, Pappas TN, McCoy CC, Englum BR, Agarwal SK, Greenberg CC
|Author Profile(s)
|J. Am. Coll. Surg. Volume: 222 Issue: 4 Pages: 515-24
|Publish Date
|2016 Apr
|PubMed ID
Because preoperative risk factor modification is generally not possible in the emergency setting, complication prevention represents an important focus for quality improvement in emergency general surgery (EGS). The objective of our study was to determine the overall impact that specific postoperative complications have in this patient population.Our study sample consisted of patients from the 2012-2013 ACS-NSQIP database who underwent an EGS procedure. We used population attributable fractions (PAFs) to estimate the overall impact that each of 8 specific complications had on 30-day physiologic and resource use outcomes in our study population. The PAF represents the percentage reduction in a given outcome that would be anticipated if a complication were able to be completely prevented in our study population. Both unadjusted and risk-adjusted PAFs were calculated.There were 79,183 patients included for analysis. The most common complications in these patients were bleeding (6.2%), incisional surgical site infection (SSI) (3.4%), pneumonia (2.7%), and organ/space SSI (2.6%). Bleeding was the complication with the greatest overall impact on mortality and end-organ dysfunction, demonstrating an adjusted PAF of 10.7% (95% CI 8.2%,13.1%, p < 0.001) and 15.9% (95% CI 13.9%, 16.7%, p < 0.001) for these respective outcomes. The only other complication with a sizeable impact on these outcomes was pneumonia (adjusted PAF of 7.9% for mortality and 13.2% for pneumonia). In contrast, complications such as urinary tract infection, venous thromboembolism, myocardial infarction, and incisional SSI had negligible impacts on these outcomes.Our study provides a framework for the development of high-value quality initiatives in EGS.
|Full Text
|Full text available on PubMed Central


text:
Kia Troubleshooting Questions? Ask a Mechanic
Welcome, I'm Chris (aka Moose).
Are you able to do electrical diag for the tachometer issue and brake repairs?
Do you have rear disc or drum bakes?
If the parking brake is not to blame then you have a faulty wheel cylinder or calipers or the brake pad mounting hardware is damaged allowing the brakes to shift and bind.
If you're confused on what to do now. You have already been charged the full question value. It's now time to compensate the tech that helped you. The only way I can continue to work here and help customers is by getting rated. If you're not pleased yet or plan not to rate me. Let me know so I can help more, close the post, or opt out. Please let me know if you're pleased with my help.
Thanks Moose.
Hello again Chris here,
Thanks Chris


text:
Personalised Slim Diary - Hollywood Star
Walk of fame!
Product Description
Key Features:
- One double page spread per week, summary calendar, UK holidays, notes and weights and measurements
- Hardback
For someone who needs a little help when it comes to all things organisation, we think our personalised diary will be a welcome gift!
Add their name to be printed inside a red star of fame against a concrete-effect background so they'll feel like an A-list celebrity each time they pen those important meetings and appointments!
You can also add a special message to appear in a white font to be printed on the back of the diary so they'll think of you each time they use it!
- Personalise it with a name in up to 25 characters and a message in up to 64 characters over two lines.
- Use our innovative preview feature to see how it will look with your personalisation. Simply follow 'Create Diary' link, type in the name and message and press the Preview button.
- H18 x W10cm
Please note:
- The diary runs from January 2017 to December 2017
Customer Reviews


text:
The bag is shaped just like a PlayStation console right down to the power and reset buttons. It has a Velcro flap fastener over the main storage compartment with a zip pocket on the back and an adjustable strap. It's even officially licensed.
See Also: The Sony PlayStation Wallet
See more pictures after the break.
Product Page ($59.99 via Gamefreaks)


text:
The Lifeline Express or the 'Jeevan Rekha Express' is the first ever 'Hospital On Rails' in the world and uses the country's railway network of over 1.15 lakh kilometers to bring medical care to the people of rural India.
Known as the 'Magic Train of India', the train has helped over 10,00,000 rural poor across the nation in almost 25 years of travelling around the nation's railway tracks.
Read on to see how this Magical train has been changing life across the nation.
A Lifeline Since 1991
The Lifeline Express is the World's First ever permanent Hospital Train. It was first established in July 16, 1991 and was later expanded to five coaches in 2007.
Helping Reduce Disabilities In India
The train is operated by Impact India Foundation, a NGO that leads the fight to prevent disabilities, and the Indian Railways who provide the coaches, the locomotive and the six member permanent crew, who call the train home.
A Volunteer Express
n all, the train has served 10,00,000 poor in rural India, restoring sight, movement, hearing and correction of cleft lips with dental and neurological treatment and more, completely free of cost in over 24 years.
The medical treatment and operations are done by groups of Doctors from across the nation who volunteer their time for the train.
A Full-Fledged Hospital On Rails
The train consists of five coaches out of which two are operation theaters while one houses the offices and sterilization autoclave for the medical equipment.
Additionally, the train has an ophthalmologic testing room, a dental unit, a laboratory, an X-ray unit and an auditorium with a large LCD display unit.
Two Operation Theatres
The first operation theatre(OT) has three operating tables and can act as standalone unit for the train while the second OT coach can be detached and used for disaster relief operations as well.
A Success Story Copied By The World
The success of the Indian Lifeline express in India has seen other countries set up similar projects in China, Central Africa as well as river boat hospitals in Bangladesh and Cambodia.
Looking To Expand
Impact India Foundation is currently looking to expand the train by 2 more coaches and has launched an appeal online for the same.


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Partners: Washington DC
Grow Your Business with a TEI Partnership
The benefits of becoming a TEI partner are numerous, with the biggest benefits being time efficient client development, the acquisition of new clients via referral, the ability to offer value-added programs with meaningful content to your clients and 24/7 outreach to CEOs. One-on-one networking events, knowledge sharing and the open exchange of ideas are just some of the ways we help deliver the benefits AND help CEOs create jobs at the same time. Want to learn more about becoming a local or national partner? Contact us today!
60i Media Productions LLC
725 Harrington Rd
Rockville, MD 20852
Phone: 301-633-2177
Andy Harper
5251 Grovemont Drive
Elkridge, MD 21075
Phone: 410-869-3253
Fax: 530-452-2266
Richard Bright
City First Bank
1432 U Street, NW
Washington, DC 20001
Phone: 202-243-7109
Mobile: 202-427-7700
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3915 National Drive
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Burtonsville, MD 20866
Phone: 301-828-4102
Fax: 301-628-4001
Robert Horenberg
Glenn Horenberg
Janet Horenberg
17218 Amity Dr
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Phone: 703-307-6912
Ross Stansfield
Saul Ewing LLP
1919 Pennsylvania Ave, NW
Suite 550
Washington, DC 20006
Phone: 202-342-3444
Fax: 202-295-6719
Mark Gruhin
Edward Levin
Snyder Cohn
11200 Rockville Pike
Suite 415
North Bethesda, MD 20852
Phone: 301-652-6700 x5488
Fax: 301-986-1028
Edward Snyder
Washington Business Journal
1555 Wilson Blvd
Suite 400
Arlington, VA 11394
Phone: 703-258-0887
James MacGregor
Jessica Altshuler
David Walker


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High class Italian style: MSC Poesia Cruise Review by jspocala
Overall Member Rating
High class Italian style
Destination: Panama Canal & Central America
Embarkation: Other
We were on a 10 night cruise to Cristobal, Panama Port. We traveled 2 1/2 days at sea, passing by Bahamas and Cuba. We were very close to Cuba and were able to see it and take pictures of its landscape. Zooming in with my camera, I was able to take very clear pictures of a light house on its shore line.
Our first stop was to the colorful Island of Curacao, N.A. Colorful it was with its pastel painted homes and its people who were very friendly and helpful. Free WIFI at a local coffee shop in town and also at the hotel near the port. Texaco has a major refinery here. There is a very tall span bridge that crosses a canal More that cuts through the island and a pontoon bridge that operates with diesel fuel outboard propeller engines. Then we left for a night time journey to Aruba. Color to the max with beautiful beaches. We had a private taxi driver tour us the island with stops to California Lighthouse and a nearby beach. Snorkel diving and swimming a must here. After another night time excursion we arrived the next day in Cartagena, Columbia. Here we had hired a private taxi for the day. $130 U.S. From the time we left the ship, he brought us to the Monastery on the mount which gave us a view of the port and the 4 islands that make up the area. He brought us to an emerald mine, the fort, the 17km wall and the beach. We spent a few hours swimming. The waves were rough. The locals on the beach try to sell you everything from beach toys, sun glasses to very creative art. You may be talked into a massage while on the beach. Then our taxi ride back to the ship. Our driver's name was Hernando and he was awesome and spoke English very well. Again, we traveled towards Panama at night to arrive early the next morning. We toured on a bus to a port on the Atlantic side of the Panama Canal. We then boarded a smaller ship that brought us through all of the locks of the canal from the Atlantic and we ended at Panama City on the Pacific coast. A bus ride back to the ship was entertaining by our guide that made us knowledgeable of the area, its history and the people. After another day at sea we came into Montego Bay, Jamaica. This island is big. A few hours is not enough time to experience the island. But colorful it is and the people are very friendly. We visited many areas but spent our time at Jimmy Buffett's Magaritaville with FREE WIFI. Back to the ship for another day at sea before arriving back to Port Everglades.
Now for the ship, The MSC Poesia. We had an awesome cabin with balcony on the 11th floor mid ship starboard side. What a view and easy access to elevators, stairwells and the pools and cafeterias. The entertainment was top shelf. The dining was better than the reviews I read and the formal dinning menu always offered you the night flare, Italian menu and always available menu. You could pick and choose from all three and you could eat all you wanted. Our waiters were Adi and Putu from Indonesia. They were awesome and energetic and made the cruise a top 5 star experience every night. This ship is not MSC's largest but is one of their biggest. The hospitality every crew member demonstrated will not be forgotten. From the cabin stewards to the Captain, all were proud to have us aboard. The deck space was plentiful and there was always a lounge chair available. I have no regrets or any negatives to mention with the exception that I wish I was still on the ship MSC Poesia cruising. Less
Published 12/13/11
Cabin review: 1111154
Great views, great location, Large cabin, plenty of drawers and closets. Large bath room with full shower. Very quiet setting. Balcony view were perfect outfitted with 2 chairs and a table. We had a sofa love seat (opens to a single bed), a pull down wall bed and a King Size bed. 2 Night tables with 3 drawers and a light on each table. A glass table in front of the sofa and a full walled make up lighted mirror over a full desk with many drawers and 2 electrical outlets. 2 110 and 2 220. We had a color flat screen tv with many channels and constant updates as to wind, speed of ship, travel maps and distances and weather conditions. Nothing was lacking. The double drapes covered the sliding glass doors to the balcony which let no sun in early in the morning. Cable internet and telephone also enhanced our cabin. Great cabin.
Port and Shore Excursions
Read Cruise Critic's MSC Poesia Review >>
First time on Sirena
Not the best from Princess
A Great Cruise On a Fantastic ...
OK but not great
Fantastic (...but don't buy th...
Good value for the price excep...
We Loved it!
Worst Cruise Ever
OK but not great
Sponsored Links


text:
Reunited and its foals so good!
NewsGeneral Equestrian NewsThursday 27 October 2016
We all had our favourite toys when we were younger, they made us feel safe, warm and were great company!
It appears it's not just humans who can have a favourite stuffed companion!
Breeze the horse was found in a poor state after his birth in May 2013.
The poor pony was shocked and dehydrated, he was taken in and cared for by the Mare and Foal Sanctuary in Devon,
Here he met Buttons the bear! Buttons gave Breeze much needed comfort and after being reunited three years later, an adult Breeze still remembers his old friend. (Photo: SWNS)
(Photo: SWNS)
Does your horse have a favourite toy? Let us know on our Facebook page!
£ 215
Latest News


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DDG::HasShareDir - Role for a plugin that has a share directory
This Moo::Role is attached to plugins which are able to give sharedir informations. A class which has no sharedir is not allowed to carry this role. The class using this role must implement module_share_dir and share. module_share_dir must return the...DDG/DDG-1017 - 31 May 2017 21:24:38 GMT - Search in distribution
1 result (0.103 seconds)


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Forums > General Industry > HELP need a more memorable name
I need an easier name to be identified with... my full name is Jasmine Sue Elliott.... Jasmine Elliott is a bit lengthy, but so is Jasmine Sue.
I will be traveling to LA soon from Grand Rapids, Michigan and I would to be able to be known by something shorter, easier more memorable then my real name.
Help?? I'm at a loss. -__-
Apr 22 13 07:56 am Link
Apr 22 13 08:02 am Link
Posts: 3152
Atlanta, Georgia, US
Jasmine Sue wrote:
Jasmine Elliot is long? Jasmine Sue is long?? It's only three syllables - how much shorter can a name be?
Apr 22 13 08:13 am Link
ME_ wrote:
I'm speaking of it being lengthy as in I don't see many well known models having that long of a name. One of my close friends is a very well known traveling model and her name is one word. Idk, just feel like I need to shorten it up to be easier to remember and make more of an impact. You know how a lot of well known alternative models have one word short names? Like Velocity? Or Mosh? That's what I'm talking about.
Apr 22 13 08:16 am Link
Posts: 3152
Atlanta, Georgia, US
Ah. So you want a word that you can use as your name; not a typical name. I would just go through the dictionary and pick out a word you like the sound of that doesn't have an undesirable meaning. Or pick out some word that is meaningful to you.
Apr 22 13 08:25 am Link
ME_ wrote:
That's what I've been trying to do for days... Having such a hard time. Lol. Thank you for your advice (:
Apr 22 13 08:27 am Link
Having the letters x or y or z in your name may make it more memorable.
It's psychology.
That's why I suggested Jazzelle.
Apr 22 13 08:29 am Link
Posts: 7337
New York, New York, US
Sue. If you want to be memorable you have to create that. It comes with a reputation not just a name. So make yourself memorable. Go work! haha
Apr 22 13 08:33 am Link
Edge of the Moon wrote:
I do agree, just always hated being called "Jazz" going up haha
Posts: 9309
Los Angeles, California, US
Jazy Sue?
Jasmine Sue wrote:
Crap, I just made an enemy.
Marin Photography wrote:
Lol, yep I know this. This forum was solely to ask for help on a name. I work very hard at modeling, its all I do, its my career. I already have made myself memorable locally, I'm traveling to LA, I'm ready to make myself memorable as I travel, and I would like a shorter, more memorable name to make an impact in a city that is FULL of memorable people. My name needs to stand out, even before they see my work.
Edge of the Moon wrote:
Haha nahhhh, you're good! (: Just siblings ya know always calling me that.. Lol
C h a r l e s D wrote:
Posts: 7337
New York, New York, US
Maybe find a flattering word that is unique to your style/genre/look...
Just peaked in your port! It's hot in there!...you trying to start a fire?...lol
Apr 22 13 08:41 am Link
Marin Photography wrote:
Why yes, yes I am. (; Hahaha.
Apr 22 13 08:45 am Link
Clothing Designer
Posts: 536
Grand Rapids, Michigan, US
Hi Jasmine! Honestly, I like the name Jasmine. It conjures in the mind images of exotic beauty and serenity. But hey, it's just my opinion. Take it for what it's worth.
Apr 22 13 09:06 am Link
Posts: 257
New York, New York, US
I think Jasmine Sue in perfect actually.
Apr 22 13 09:09 am Link
Posts: 7699
Buffalo, New York, US
Jasmine Sue wrote:
Hate to say it, but I keep thinking, "Jasmine T".
Posts: 22737
Orlando, Florida, US
SuJay . . . Once they see yer pix, they'll remember ya . . . don't worry . . .
Posts: 5210
Nottingham, England, United Kingdom
Jessi Belle
Elli Ott
Elli Belle
Jessa Bell
Apr 22 13 09:57 am Link
Jasmine-Sue Aryan-Nation Hitler.
Guaranteed to get you remembered.
Apr 22 13 10:11 am Link
Posts: 1254
Heerlen, Limburg, Netherlands
RKD Photographic wrote:
Only someone in Germany could get away with saying this
Apr 22 13 10:20 am Link
--Ishtar-- wrote:
Oh I don't know - he had a memorable impact on your side of the border too, as I understand from my numerous visits around Nijmegen and Arnhem......
Apr 22 13 10:24 am Link
Drew Smith Photography wrote:
I only just noticed this...
Apr 22 13 10:25 am Link
sospix wrote:
I actually really like this! Thank you (:
Chain Reaction wrote:
D'awww thank you.
Good chance I'm keeping Jasmine Sue, thanks guys!!!! I appreciate all the help (:
Apr 22 13 10:36 am Link
Posts: 1254
Heerlen, Limburg, Netherlands
RKD Photographic wrote:
Yep, but most Dutch people would probably not be so flip about the whole thing for that reason, esp. those that saw the war up close.
Apr 22 13 10:57 am Link
Posts: 1
Orlando, Florida, US
Jazzi Sue?
Apr 22 13 11:35 am Link
Clothing Designer
Posts: 4448
Bakersfield, California, US
Looking at your port, I took a little of your ink and a little of your name: TatSue!
Apr 22 13 11:40 am Link
Posts: 1
East Rochester, New York, US
some of the models i have worked with have had for lack of a better word " stalker " " problems off and on don't connect your modeling name to your real name. it makes it harder to find you on the internet think safety keep your personal and modeling info seperate
Apr 23 13 07:23 pm Link
Posts: 2590
Chicago, Illinois, US
Personally I like Jasmine Sue.
There's a Johnny cash song, "A Boy Named Sue" about him wondering why he had what he thought was a girls name. Daddy left him. Had to fight all his life about that name. Grew up with hard fists, a rambler, looking to kill his daddy for giving him that name. Walked into a saloon later in life. Ran into his daddy. Ready to kill him. They fight. His daddy cuts off a piece of his ear. Turns out they both like to fight. They pull their guns. Daddy says son, this world is rough and I knew I wouldn't be there to help you a long. You got the right to kill me, but maybe you should be thanking me, for being the son of a b* that named you Sue.
Apr 23 13 07:34 pm Link
Posts: 765
Temple City, California, US
JSue what up hope your find a catchy name.
Posts: 39245
Portland, Oregon, US
How about "memorable", "memorable sue', or "memory"
Posts: 7891
Monterey, California, US
(a play on your initials pronounced as Jay-ess)
Apr 23 13 07:41 pm Link
Posts: 198
Newcastle, New South Wales, Australia
Apr 23 13 07:52 pm Link
Posts: 928
Austin, Texas, US
I like your name as it is.
It is a mix of a more recently popular first name and a traditional middle name. To me, you sound young but grounded based on your given name.
Apr 23 13 08:08 pm Link
Posts: 606
Boston, Massachusetts, US
Svetlana Muerte wrote:
totally agree with this...
Apr 23 13 08:22 pm Link
Posts: 22
Eugene, Oregon, US
Sue Elliot
Apr 23 13 08:40 pm Link


text: It is all part of Physics. The water molecules find it hard to escape when the air (which boiled water is part of, being a gas) is compressed. It is the same reason is why the sun is only 5000 degrees on the surface but several million in the heavy core.


text:
New Winter Fashion Collection - 100% Merino Wool
A stylish range featuring lovely hues of grey and pastels that can be mixed and matched to create your own outfit.
Fine Grey Striped Pants for mixing and matching with other styles.
Click Here to read more about the special properties of Dimples NZ Merino Wool
Click here to view our size guide
Follow us on Facebook
For 20 Years Dimples has been your trusted babywear brand. Our garments are all made to very high quality standards and are proudly 100% made in New Zealand.


text:
Research Projects
Raise Your Voices! The Occurrence of Nonviolent Campaigns in Civil Wars
Civilians are influential, often pivotal, actors in civil wars. They are not only passive or victims of violence, but rather use the public to raise their voices. Paradoxically, civilians voice their interests often in an organized and nonviolent way. Such nonviolent campaigns address both the government and the rebel group(s) in civil wars, as shown by examples from different violent intra-state conflicts.
The project makes the following contributions: (1) The project aims to contribute to the growing research on nonviolent campaigns by widening the theoretical perspective to nonviolent campaigns in the context of civil wars, to the occurrence of campaigns and to campaigns below the threshold of 1,000 participants. (2) By collecting geo-referenced data, the project aims to account for spatial variations within civil wars and nonviolent campaigns. (3) The project findings could be relevant for policy advice. Nonviolent campaigns are signals showing the willingness of civilians to take responsibility for their own future and they increase the likelihood of future activism. Thus, former campaign activists will very likely take responsibility for local developments in the post-conflict period. Communities that have experienced nonviolent activism therefore should be promising settings for development projects in the post-conflict period.
The project will use quantitative and qualitative methods on different levels of analysis to study the proposed mechanisms empirically. We will conduct in-depth studies of two civil war cases to identify civilian nonviolent activism in civil wars.
Project details
Principal Investigator: Johannes Vüllers
Funded by the Deutsche Forschungsgemeinschaft (DFG)
Duration: 21.12.2015 – 30.06.2018
Amount of funding: EUR 310.000
Project members: Roman Krtsch, Kai Merkel
The Web as a Curse or Blessing? Ethnic Mobilization in the Information Age (Sofja Kovalevskaja Award)
Project details
Principal Investigator: Nils B. Weidmann
Funded by the Alexander von Humboldt Foundation
Duration: 01.10.2012 – 30.09.2017
Amount of funding: EUR 1.4 Million
Project members: Sebastian Hellmeier, Annerose Nisser, Espen Geelmuyden Rød


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Sidharth Malhotra will be stepping away from his image of a lover boy and will be seen in a grumpier avatar in 'Brothers'. But Sid says more than an action film, 'Brothers' was an emotional roller-coaster for him.
Says the Bollywood hunk, "Brothers is not just an action film. It is a story of two brothers and the turmoil they face when they have to face each other in the boxing-ring. People who have seen 'Warriors' know the story, but it does not affect the emotional quotient of the film. I can honestly say, Brothers will make men cry."
The film is slated to hit the big screens on 14th August 2015.
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Question: Briefly describe the guidelines for recognizing revenue from the
Briefly describe the guidelines for recognizing revenue from the sale of software and other multiple-deliverable arrangements.
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Answer to relevant QuestionsBriefly describe how IFRS guidelines for recognizing revenue from multiple-deliverable arrangements differ from U.S. GAAP guidelines.Interim reports are issued for periods of less than a year, typically as quarterly financial statements. Should these interim periods be viewed as separate periods or integral parts of the annual period?Refer to the situation described in BE 5-6. During the first year the company billed its customer $7 million of which $5 million was collected before year-end. What would appear in the year-end balance sheet related to this ...The 2011 income statement for Anderson TV and Appliance reported sales revenue of $420,000 and net income of $65,000. Average total assets for 2011 was $800,000. Shareholders' equity at the beginning of the year was $500,000 ...Sanchez Development Company uses the installment sales method to account for some of its installment sales. On October 1, 2011, Sanchez sold a parcel of land to the Kreuze Corporation for $4 million. This amount was not ...
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