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Dr. George Fyffe Jr MD Patient Reviews
- Obstetrics and Gynecology Vero Beach, FL
Overall Rating
23 Patient Reviews
- Friendly Staff
- Promptness
- Wait Time 15 minutes
- Appropriate Followup
- Spends Time With Patients
- Bedside Manner
- Fair and Accurate Diagnosis
- Easy Appointments
Showing 1 - 12 reviews
5.0 of 5
April 22nd, 2015
I switched drs when I was 7 months pregnant and Dr. Fyffe was a kind and gentle man. I had complications when I was in labor and he made the decision to do an emergency c-section. I felt ok and at ease in his decisions under my circumstances and am thankful because he saved my baby girls life! He was gentle yet professional and handled all the situations well. Thank you Dr. Fyffe! My c-section incision site looks beautiful.
5.0 of 5
February 3rd, 2014
Great doctor!
Dr Fyffe was my doctor in Fort Lauderdale 10 years ago and when he left I was so upset. Best MD ever! My husband went to every single doctors visit with me and boy, dr Fyffe had his hands full !!! We miss you and wish you well! Very caring and I would recommend him as a doctor!!
5.0 of 5
August 21st, 2013
My First child and best DR ever!
Dr.Fyffe was not only professional and down to earth and friendly. He ALWAYS made time for me and my questions. There are other doctors at that office but after a few meetings with him I began to request him. This might be weird to say but he is so gentle! I have had countless Pap smears and exams (like every women) but it is totally different with him, it doesn't hurt! I normally cry from an exam but with him it is fast, easy and painless. And he makes you feel very comfortable. I'm pregnant with #2 and wish I was still in Vero so he could be my doctor. I'm doctor hunting now and he made it impossible to find anyone that stands up to his standards. I would HIGhLY recommend and the office! The office is always clean and the staff is so sweet, especially pandora and Victoria!
5.0 of 5
May 30th, 2012
wish he was still in miami
The best doctor thus far....he was my OBGYN in Miami...made my first time mother fears go away.....very careful inassuring a healthy and normal delivery....thus was in 2003....I have yet to find better...
5.0 of 5
November 12th, 2011
Very nice office staff and doctor..made my visit very comfortable through showing care and skill.
5.0 of 5
August 3rd, 2011
emergency surgery
very professional, compassionate, he saved my ovaries.
5.0 of 5
March 28th, 2017
5.0 of 5
July 13th, 2016
5.0 of 5
June 6th, 2016
5.0 of 5
March 4th, 2016
1.0 of 5
December 1st, 2015
5.0 of 5
October 10th, 2015
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Fry in pan with little oil, 4 spoons channa dal, 4 red chillies, 6 tomato cut pieces. Once done, grind with salt and hing . Temper with mustard (in gingelly oil).
In thogayal, we normally use urad dal, this is different that we use channa dal, one of my mom's speciality , she makes very frequently.

subset: commoncrawl


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Lyrics Depot is your source of lyrics to Dont Let Him Go by REO Speedwagon. Please check back for more REO Speedwagon lyrics.
Dont Let Him Go Lyrics
Artist: REO Speedwagon
Don't Let Him Go
REO Speedwagon
So you figure that you've
Got him all figured out,
He's a sweet talking stud
Who can melt a girl's heart with his pout.
He's the kind of lover that the ladies dream about.
Oh, yes he is.
He's got plenty of cash,
He's got plenty of friends,
He drives women wild,
And he drives off in a Mercedes Benz,
He's got a long wick with a flame at both ends.
He's hot.
But don't let him go.
Just give him a chance to grow.
Take it easy take it slow,
But don't let him go.
Don't let him go.
He makes you so angry,
He makes you so sore.
The wait may be worth it,
But how can you wait anymore?
When you're wondering what you're waiting for?
Baby, I don't know.
From: "David Garnsey"
REO Speedwagon Lyrics
More REO Speedwagon Music Lyrics:
REO Speedwagon - Building The Bridge Lyrics
REO Speedwagon - Gary's Guitar Solo Lyrics
REO Speedwagon - Give Me A Ride (Roller Coaster) Lyrics
REO Speedwagon - Let's Be-Bop Lyrics
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REO Speedwagon - Ridin' The Storm Out Lyrics
REO Speedwagon - The Key Lyrics


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Question: Contrast supply chain effectiveness and supply chain efficiency
Contrast supply chain effectiveness and supply chain efficiency.
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It feels like it's been a while since there were any rays of hope in the news as of late, but for what seems like the first time in a long time, we've got some – the US Army Corps of Engineers will begin looking for an alternate route for North Dakota Access Pipeline.
In what is a massive victory for the Standing Rock Sioux Tribe and peaceful protestors everywhere, The Department of the Army announced on December 4th that it would not allow the Dakota Access Pipeline to be drilled under a dammed section of the Missouri River. Instead, the US Army Corps of Engineers said it would seek alternative paths to construct the pipeline, which means that the sacred lands of Standing Rock on the Sioux Tribe reservation lands will be left unfettered by the construction and routing of the pipeline.
The $3.7 billion "DAPL" project had quickly become a point of international emphasis, as stories of peaceful protestors being harassed and harangued by Army reservists and sheriff's deputies began to make their way out into the ether. The growing concern over disappearing Native Lands brought thousands to the front lines of Standing Rock as Sioux Tribe members protested peacefully in defense of their land, in turn bringing attention to the vast teepee and yurt laden lands that were once in limbo.
Energy Transfer Partners – the company funding a large portion of the DAPL initiative and handling most of the day to day operations – had no immediate response to the US Army Corps of Engineers' decision, but the company has said in the past that they would not necessarily entertain the notion of seeking different routes for constructing the pipeline. They are now left with no other option to but reroute the pipeline which would transport nearly 550,000 barrels of oil a day to a terminal point in Illinois.
On the side of the protestors, relief would be an understatement, as many environmental groups are applauding the US Army Corps of Engineers' decision and hailing the Standing Rock Water Protectors as heroes for their efforts. The decision brings double relief to those found in the Oceti Sakowin campsite just outside the frontlines of the pipeline construction site, as any further extension of the standoff would have forced protestors to suffer through the brutal North Dakota winter.
The protestors at Standing Rock – led by the aforementioned Standing Rock Water Protectors – had argued that the pipeline's projected path had been routed too close to their drinking water, stating that any spill could conceivably poison their primary source of drinkable water. With news of the US Army Corps of Engineers' decision, many let out a final rallying cry of the Water Protectors – "Mni wiconi!" which means "water is life," in the Sioux native tongue.
While the initial conflict has been resolved, this unfortunately may not be the last we see of the DAPL conflicts popping up in the future. Many will remember the brief allegations of physical harassment and harm between protestors and the local Sheriff's deputy office at Standing Rock, whose demonstrations had been labeled as unlawful protest. Furthermore, many associated with Energy Transfer Partners have bemoaned the decision as being wholly political, and they remain hopeful that president-elect Donald Trump will look to reconsider the DAPL after his inauguration.Trump has said in the past that he supports the construction of the pipeline (but it should be noted that Trump owns stock in Energy Transfer Partners). So there is still plenty to sift through in the coming weeks with regard the pipeline's future, but for the time being, the one thing we do know is that it will not route through Standing Rock.
#NoDAPL Dakota Access Pipeline dakota access pipeline blocked
Last modified: December 6, 2016


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Human glomerulonephritis (GN) is characterized by sustained proteinuria, sodium retention, hypertension, and edema formation. Increasing quantities of filtered protein enter the renal tubule, where they may alter epithelial transport functions. Exaggerated endocytosis and consequent protein overload may affect proximal tubules, but intrinsic malfunction of distal epithelia has also been reported. A straightforward assignment to a particular tubule segment causing salt retention in GN is still controversial. We hypothesized that 1) trafficking and surface expression of major transporters and channels involved in volume regulation were altered in GN, and 2) proximal tubular endocytosis may influence locally as well as downstream expressed tubular transporters and channels. Effects of anti-glomerular basement membrane GN were studied in controls and megalin-deficient mice with blunted proximal endocytosis. Mice displayed salt retention and elevated systolic blood pressure when proteinuria had reached 10–15 mg/24 h. Surface expression of proximal Na+-coupled transporters and water channels was in part [Na+-Pi cotransporter IIa (NaPi-IIa) and aquaporin-1 (AQP1)] increased by megalin deficiency alone, but unchanged (Na+/H+ exchanger 3) or reduced (NaPi-IIa and AQP1) in GN irrespective of the endocytosis defect. In distal epithelia, significant increases in proteolytic cleavage products of α-epithelial Na+ channel (ENaC) and γ-ENaC were observed, suggesting enhanced tubular sodium reabsorption. The effects of glomerular proteinuria dominated over those of blunted proximal endocytosis in contributing to ENaC cleavage. Our data indicate that ENaC-mediated sodium entry may be the rate-limiting step in proteinuric sodium retention. Enhanced proteolytic cleavage of ENaC points to a novel mechanism of channel activation which may involve the action of filtered plasma proteases.
- proximal tubule
- collecting duct
- megalin
- ENaC
- NaPi-lla
- proteolytic cleavage
proteinuria is a clinical hallmark of acute and chronic glomerular diseases. Under normal conditions, glomerular permselectivity is believed to retain most serum proteins. Only minor quantities especially of albumin are thus passing through the filter to be retrieved by the proximal tubule via receptor-mediator endocytosis (RME), requiring the multiligand receptors megalin and cubilin (15). Proteins are subsequently delivered to the lysosomes, degraded, and finally delivered to the peritubular blood. In nephrotic range proteinuria, substantially more protein is filtered through the damaged glomeruli owing to the general increase in fractional filtration of macromolecules. The resulting high tubular protein concentration leads to saturation of the receptors and exceeds the retrieval and degradation capacity of the proximal tubule, causing cellular protein overload and proteinuria. Interstitial edema, renal salt and water retention, and hypertension occur consequently (17, 35, 42). The potential causes for these disorders have been only partly elucidated to date.
Salt retention in nephrosis has commonly been explained by the hypovolemia concept, implying decreased oncotic pressure following proteinuria, loss of fluid into the interstitium, and subsequent activation of the renin-angiotensin-aldosterone system (RAAS) (18, 32). However, volume disorders in nephrotic patients may also occur without concomitant changes in blood pressure and effects of the RAAS (3, 38). It has therefore been discussed that primary tubular salt retention may per se lead to volume expansion ("circulatory overfilling") (27, 42).
In the search for potential sites of sodium retention along the renal tubule, the proximal tubule has been a focus (1, 4, 24, 34, 51). Protein overload may impair its structure, causing cytoskeletal changes, lipids may become accumulated, and inflammatory factors can be induced. As a result, trafficking of transporters and channels may be impaired (22, 32, 40). Na+/H+ exchanger 3 (NHE3)-dependent sodium reabsorption and lysosomal acidification may be affected (7, 32, 34), and coexpressed transport proteins such as aquaporin-1 (AQP1) and the Na+-Pi cotransporter IIa (NaPi-IIa) may be involved (22).
Distally, regulation of the Na+-K+-2Cl−-cotransporter 2 (NKCC2) of thick ascending limbs (TAL) of Henle's loop may be affected as well (32), but the major distal site of retention has formerly been assigned to the collecting ducts (6, 29), and recent work has been concentrated on the activation of the epithelial Na+ channel (ENaC) in a RAAS-independent way (3, 33, 38). New perspectives have also come from observations that ENaC subunits are cleaved by proteases, which may increase the open probability of the channel (3, 5, 13, 21, 22, 25, 28, 43, 48; for a review, see also Ref. 44).
The aim of this study was to specify possible tubular causes for volume disorders in rapid-progressive, immune-mediated glomerulonephritis (GN) under controlled proteinuria which was clearly below nephrotic range. Changes at the level of the proximal and distal nephron epithelia were analyzed. We hypothesized that 1) trafficking and surface expression of the major tubular transport proteins involved in volume regulation were altered in GN, and 2) proximal tubular RME with respect to its high protein reabsorptive capacity may influence locally as well as downstream expressed tubular transporters and channels. To this end, an established mouse model for anti-glomerular basement membrane (GBM) GN, induced in a kidney-specific, megalin-deficient transgenic strain (36, 51), was used. The partial (50–70%) absence of a functioning, megalin/cubilin-mediated endocytotic apparatus in this strain permits the side-by-side analysis of megalin-deficient and intact proximal tubules for histological analysis and causes low-molecular-weight proteinuria (36). This constellation was used to define the proximal and distal phenotypes of the membrane transporters in GN-grade glomerular proteinuria, with supplementary implications from tubular proteinuria.
Animals and treatments.
Experiments were performed in female conditional megalin knockout mice (megalin lox/lox; apoE Cre) (51), here termed Cre(+), and in controls (megalin lox/lox), termed Cre(−); body weight 20–30 g, age 12–15 wk. Remnant megalin expression in Cre(+) was verified by sampling of the urinary vitamin D binding protein levels (36). Mice [n = 25; 12 Cre(+) mice and 13 Cre(−) mice] were immunologically primed by subcutaneous injection of rabbit IgG in complete Freund's adjuvant. GN was induced 6 days later by the intravenous injection of an anti-mouse GBM serum (47) in six Cre(+) and seven Cre(−) mice, whereas six mice of each strain received an injection of vehicle. The mice were killed when proteinuria had reached 10–15 mg/24 h, and control animals were killed in parallel. Mice were allowed free access to standard chow and tap water. For urine analysis, mice were individually placed in metabolic cages for 24 h before day 0 and every week starting from day 12 of the experiment. The experiments were conducted in accordance to the German Law for the protection of animals (Reg G 0178/03).
Blood and urine analyses.
Electrolytes in serum and urine were determined by indirect ion-selective electrode measurements (Modular Analytics, Roche Diagnostics, Mannheim, Germany), and urinary osmolality was measured with an osmometer (Gonotec, Berlin, Germany). Blood urea nitrogen (BUN) and creatinine concentrations were quantified enzymatically and by the kinetic Jaffé-method (Modular Analytics, Roche Diagnostics). Creatinine clearance and fractional sodium excretion were calculated using standard equations. Total protein concentration in serum and urine as well as albumin, cholesterol, and triglyceride levels in serum were measured with standardized autoanalyzer methods (Hitachi 747, Hitachi 911, and STA analyzers; Roche Diagnostics).
Hemodynamic measurements.
At the end of the experiment, catheters were inserted into the right femoral artery. Blood pressure recordings were performed 24 h after surgery in conscious unrestrained mice. Animals were observed during the recording period, and recording was stopped once an artifact-free recording of at least 5 min was obtained (47).
Fixation and tissue processing for immunohistochemistry.
Mice were anesthetized by an injection of pentobarbital sodium (0.06 mg/g body wt ip). Kidneys were then perfused retrogradely through the abdominal aorta using 3% paraformaldehyde (PFA) as described (51) for immunohistochemistry or removed, weighed, and shock-frozen for biochemistry. For cryostat sectioning, tissues were protected from freezing artifacts by subsequent overnight immersion in PBS adjusted to 800 mosmol/kgH2O sucrose, shock-frozen, and stored at −80°C. For the paraffin technique, tissues were postfixed in 3% PFA, dehydrated, and standard paraffin-embedded and stored at −20°C.
Tissue processing for immunoblotting.
For isolation of cortical brush border membrane (BBM), the kidney cortex was homogenized in isolation buffer containing 300 mM d-mannitol, 5 mM EGTA, 16 mM HEPES, 10 mM Tris-base, and a protease inhibitor cocktail (Complete, Roche Diagnostics) as described (4). For the isolation of cortical and medullary membrane fractions, tissue was homogenized in sucrose buffer containing 250 mM sucrose, 10 mM triethanolamine, and a protease inhibitor cocktail (Complete) and centrifuged to obtain the plasma membrane fraction. Total protein concentration was measured using the Pierce BCA Protein Assay reagent kit (Pierce, Rockford, IL) and controlled by Coomassie staining.
Gel electrophoresis and SDS-PAGE.
After Laemmli's sample buffer was added, the proteins were solubilized at 96°C for 3 min. SDS gel electrophoresis was performed on 8–10% polyacrylamide gels. After electrophoretic transfer of the proteins to nitrocellulose membranes, equity in protein loading and blotting was verified by membrane staining using 0.1% Ponceau red. Membranes were probed overnight at 4°C with primary antibodies and then exposed to horseradish peroxidase-conjugated secondary antibodies (DAKO, Hamburg, Germany). Immunoreactive bands were detected on the basis of chemiluminescence, using an enhanced chemiluminescence kit (Amersham Pharmacia, Freiburg, Germany) before exposure to X-ray films (Hyperfilm, Amersham). For densitometric evaluation of the resulting bands, films were scanned and analyzed using BIO-PROFIL Bio-1D image software (Vilber Lourmat, Marne-La-Vallée, France). All parameters had been normalized to β-actin abundance.
We used previously well-characterized antibodies: rabbit anti-AQP1 (BD Biosciences), monoclonal mouse anti-NHE3 (Chemicon International), rabbit anti-NaPi-IIa, raised against an NH2-terminal peptide sequence; guinea pig anti-megalin (generated against a COOH-terminal peptide), monoclonal mouse anti β-actin (Sigma, Deisenhofen, Germany), rabbit anti-AQP2 (gift from E. Klussmann), monoclonal mouse anti-α1-subunit of Na+-K+-ATPase (α-NKA; Upstate Biotechnology), guinea pig anti-NKCC2 (generated against an NH2-terminal peptide sequence); and α-, β-, and γ-subunits of ENaC (sera produced against NH2-terminal glutathione-S-transferase protein of the α-subunit and COOH-terminal glutathione-S-transferase proteins of β- and γ-subunits of ENaC), rabbit anti-cyclooxygenase-2 (COX-2; Cayman Chemical), and rabbit anti-renin (gift of A. Kurtz).
Immunohistochemical staining was performed on cryostat or on paraffin sections. Sections were blocked with 5% skim milk/PBS, incubated with the respective primary antibody (see antibodies listed above), followed by incubation with suitable horseradish peroxidase-coupled secondary antibodies (DAKO) or cy-3-coupled secondary antibodies (Dianova, Hamburg, Germany). In double-labeling techniques, the different primary antibodies were administered consecutively. Specificity of the double-staining procedures was controlled by parallel incubation of consecutive sections, each incubated only with one single probe. Sections were counterstained and analyzed using a Leica DMRB microscope or multilaser confocal scanning microscope (TCS SP-2, Leica Microsystems, Bensheim, Germany).
RNA isolation, reverse transcription, and real-time PCR.
Total RNA was extracted from kidney cortices using an RNeasy Mini kit (Qiagen, Hilden, Germany) and treated with DNase I (Invitrogen). Reverse transcription was performed with the Super-Script First-Strand Synthesis System for RT-PCR (Invitrogen). TaqMan Gene Expression Assays were used, and the product IDs for the candidate genes are Mm00431834_m1 (AQP1), Mm01352473_m1 (NHE3), and Mm00441450_m1 (NaPi-IIa). The experiment was performed according to the manual provided by Applied Biosystems (Foster City, CA).
NHE activity measurements.
NHE activity was determined in BBM vesicles by the acridine orange technique as described (11). Measurements were performed in a Shimadzu RF-5000 spectrofluometer equipped with a thermostatized cuvette (kept at 25°C). BBM vesicles were dissolved in a buffer containing 280 mM mannitol, 5 mM Mes, and 2 mM MgCl2 (adjusted to pH 5.5 with N-methyl-d-glucamine). Acridine orange was excited at 493 nm, and emission was monitored at 530 nm. The cuvette was filled with 2 ml of buffer (240 mM mannitol/20 mM HEPES/2 mM MgCl2, adjusted to pH 7.5 with N-methyl-d-glucamine), containing 6 μM acridine orange. The experiment was started by injecting 30 μl of BBM vesicle suspension. After 60 s of equilibration, NHE activity was initiated by injection of 80 μl of 2 M Na gluconate. NHE activity was calculated as the ratio of ΔpH per min over Q, where Q is the initial quenching after injection of BBM vesicles. All experiments were done at least in quadruplicate and repeated four times with BBM vesicles from two animals per experiment. To block specifically NHE3, 100 μM EIPA was used.
Quantification of COX-2 and renin content.
Histochemical signals were semiquantitatively evaluated with a ×20 and ×40 objective to establish changes in COX-2 and renin abundance, respectively. The mean number of renin-positive sites or COX-2-positive macula densa cells at the juxtaglomerular apparatus was determined within an area of 100–150 glomeruli on average (49).
Aldosterone assay.
Plasma aldosterone was measured with a commercial kit (COAT-A-COUNT, Diagnostic Products). The detection limit was 13.0 pg/ml, and the intra-assay coefficient of variation was <4%.
Presentation of data and statistical analysis.
Quantitative data are presented as means ± SE. For statistical comparison, the Mann-Whitney U-test was employed. P values of <0.05 were considered statistically significant, with (*) demonstrating significance between Cre(−) and Cre(+) and (#) between control and GN mice.
Control mice [Cre(−)] and megalin-deficient mice [Cre(+)] as well as control mice with GN [Cre(−)/GN] and megalin-deficient mice with GN [Cre(+)/GN] were compared. Clinical and renal functional data are presented in Table 1. Control Cre(+) mice had significantly elevated urinary protein excretion owing to the known low-molecular-weight proteinuria in this strain (36). Application of the nephritogenic antibody to Cre(−) and Cre(+) induced GN with marked proteinuria as established earlier (5). All GN mice had been killed when proteinuria had reached similar levels between strains and tubulointerstitial changes had not yet developed. Baseline blood pressure in Cre(+) was slightly higher than in Cre(−) but did not reach significance (90.2 ± 3.6 vs. 78.2 ± 5.5 mmHg; P = 0.10). Induction of GN led to significant blood pressure increases in both strains with a higher tendency maintained in Cre(+)[113.3 ± 6.7 in Cre(−)/GN and 120.3 ± 6.9 in Cre(+)/GN; P < 0.05 intrastrain, P = 0.12 interstrain] (Fig. 1). Tissue COX-2 and renin immunoreactivity levels, as revealed by juxtaglomerular quantification of histochemical signals, and plasma aldosterone concentrations showed no significant differences between groups (Table 2). The fractional sodium excretion was strongly reduced with GN in both strains (P < 0.05). Plasma cholesterol levels were increased in GN. Since Cre(+) had the characteristic mosaic pattern of megalin deficiency (4), the degree of megalin knockout was monitored by Western blotting of BBM, revealing between 50 and 70% megalin deficiency in this strain. Urinary vitamin D binding protein (DBP) excretion was determined to assess the influences of proteinuria and megalin-dependent endocytosis on the tubular handling of this parameter (Fig. 2). As expected, DBP was significantly increased in megalin deficiency [916 ± 99% in Cre(+) vs. 100 ± 32% in Cre(−); P < 0.05]. The induction of GN caused a marked increase in DBP excretion in Cre(−)/GN group (782 ± 111%; P < 0.05), which was still more pronounced in the Cre(+)/GN (1,371 ± 143%; P < 0.05). In sum, RME deficiency alone produced mild proteinuria. The GN groups were characterized by substantial proteinuria, elevated blood pressure, proteinuria, and sodium retention; RME deficiency only tangentially but not significantly aggravated these parameters. Juxtaglomerular and RAAS parameters were unchanged.
Abundances of proximal tubular transporters and AQP1.
Megalin-positive and megalin-deficient proximal tubule cells were compared side-by-side under control and proteinuric conditions. Figure 3 demonstrates that the absence of megalin and associated changes in cell morphology produced no major changes in NHE3 immunoreactive signals at the base of the BBM, whereas NaPi-IIa and AQP1 signals were increased apically in BBM of Cre(+) compared with Cre(−). NHE3 activity, as measured in BBM vesicles using the acridine orange quenching method, showed no differences between the control groups; using the NHE3-specific inhibitor EIPA (100 μM), total NHE activity was entirely blocked in Cre(−) and Cre(+) alike (Fig. 4). BBM Western blot abundances of NaPi-IIa and AQP1, but not of NHE3 were higher in Cre(+) (Fig. 5A; Table 3), whereas mRNA levels of all three products were unchanged (Table 4).
The induction of GN produced no changes in proximal tubular NHE3 immunoreactive signals irrespective of the presence of megalin (Fig. 3). In contrast, NaPi-IIa and AQP1 signals were markedly decreased in Cre(−)/GN, and in Cre(+)/GN, megalin-positive as well as megalin-negative cells showed reduced signal intensities compared with Cre(+). Total NHE activity in the GN groups was not different from the controls; however, since blockade by EIPA indicated a significant reduction of NHE3 activity [44.2 ± 3.1% in Cre(−) and 42.5 ± 4.9% in Cre(+); Fig. 4], yet another NHE activity, which remained unaffected by EIPA, may have become activated upon GN induction. These changes were not influenced by megalin availability. BBM Western blot intensity mostly paralleled these changes (Fig. 5A; Table 3). Cortical mRNA levels were unchanged for NHE3, whereas NaPi-IIa and AQP1 showed a reduction in GN that did not depend on megalin (Table 4).
In aggregate, these results show that cortical NHE3 synthesis and BBM abundance are largely unaffected by proteinuria as well as megalin-dependent endocytosis, whereas NHE3 activity was reduced in GN. Contrastingly, the abundances of NaPi-IIa and AQP1 were mostly reduced under GN-associated proteinuria, even though megalin-deficient cells showed the established, relative signal enhancement for NaPi-IIa caused by its impaired retrieval (4), and the same applied to AQP1. The details of these changes are summarized in Table 5.
Abundances of distal tubular transporters and AQP2.
To study the epithelial effects of GN on transporter proteins of the distal tubule and collecting duct, the expression and cellular distribution of NKCC2, α-ENaC, β-ENaC, γ-ENaC, α-NKA, and AQP2 were evaluated (Table 3). Western blot quantifications were performed in cortical or medullary extracts of the plasma membrane in analogy to the BBM fractions, since it was assumed that the abundance of these transporters at their site of action indicates their activation.
Negative results were obtained with NKCC2 and α-NKA. Cortical and medullary parts of TAL segments showed no changes among all groups in NKCC2 plasma membrane abundance, and neither was total α-NKA altered in cortex or medulla (Table 3). In contrast, α-ENaC and γ-ENaC showed significant, selective enhancements not of their major, characteristic full-length bands, but of additional, lower molecular weight bands, which probably represented cleavage products of these ENaC subunits.
At steady state, the additional bands at ∼55 and 30 kDa were 3.74- and 1.26-fold enhanced for cortical α-ENaC in Cre(+) compared with Cre(−), suggesting that the absence of megalin and lack of proximal RME had led to a higher distal yield of filtered plasma components possibly acting on ENaC structure (Fig. 5B). Similarly, but less pronounced, an additional band for cortical γ-ENaC at 70 kDa was selectively stronger in Cre(+) (Fig. 5B). Additional α-ENaC bands in the medulla were enhanced as well (Fig. 5C). There were no changes in allover β-ENaC or medullary γ-ENaC expression (Table 3).
In GN, the 55- and 30-kDa band densities for cortical α-ENaC were 5.08- and 1.51-fold enhanced in Cre(−)/GN and 7.44- and 1.89-fold in Cre(+)/GN, respectively (Fig. 5B). For medullary α-ENaC, the 55- and 30-kDa band densities were 3.5- and 1.75-fold increased in Cre(−)/GN and 4.79- and 2.0-fold increased in Cre(+)/GN, respectively (Fig. 5C). These results thus further underline the impact of endocytosis on nephritis-grade distal tubular proteinuria and its possible modification of α-ENaC. Cortical γ-ENaC was also showing increases in the 70- and 55-kDa bands in GN, but the effects of endocytosis were not detectable [70 kDa: 2-fold increases in Cre(−)/GN and 2.2-fold in Cre(+)/GN; 55 kDa: 4.1- and 4.31-fold increases each in Cre(−)/GN and Cre(+)/GN]. Again, there were no changes in the medulla for γ-ENaC or in total β-ENaC expression (Table 3).
Expressed as total densities of the full-length and lower molecular weight bands, compared with Cre(−) the increases in Cre(+), Cre(−)/GN, and Cre(+)/GN were 4.16-, 4.76-, and 8.49-fold for cortical α-ENaC and 1.57-, 4.1-, and 5.76-fold for medullary α-ENaC, respectively (each P < 0.05; Table 3), displaying significant increases also between Cre(−)/GN and Cre(+)/GN (P < 0.05). For cortical γ-ENaC, increases were 3.0-, 4.1-, and 4.31-fold in Cre(+), Cre(−)/GN, and Cre(+)/GN, respectively (P < 0.05; Table 3), but differences between Cre(−)/GN and Cre(+)/GN were not significant. Together, total density values indicate increased abundances of ENaC subunits in distal membranes as a function of different grades of proteinuria.
The signals for AQP2 were strongly diminished in the cortex but not the medulla of Cre(−)/GN and Cre(+)/GN (Fig. 5B; Table 3).
These findings were roughly paralleled by changes registered in the immunohistochemical staining series (Fig. 6). The adluminal staining for cortical collecting duct α-ENaC and γ-ENaC was increased in Cre(+) compared with Cre(−). Cortical α-NKA and AQP2 signals were not markedly different between Cre(−) and Cre(+). In GN, Cre(+) showed stronger adluminal ENaC signals than Cre(−). α-NKA signals showed massive increases in GN; this differed from the Western blot analysis (Table 3), representing a mixture of all segments so that α-NKA changes in the collecting ducts were probably masked. AQP2 signals were diminished in the GN groups.
This study adds new insights to the discussion of whether primary, kidney-based salt and water retention, or other, systemic causes prevail in the volume disorders accompanying heavy proteinuria. Our results have demonstrated that the blunted proximal RME and resulting mild proteinuria as well as the GN-induced glomerular proteinuria with or without proximal RME had little impact on proximal tubular membrane transporters with respect to volume retention. No changes were encountered in the loop of Henle. In contrast, both measures have caused major, in part additive, changes in surface expression and molecular weight shifts of ENaC subunits suggestive of channel activation and potential salt retention. Significant increases in blood pressure, decreased fractional sodium excretion, and increased plasma cholesterol levels were established in the GN groups. By stabilizing proteinuria within limits of 10–15 mg/24 h in these groups, we could avoid changes in GFR which occur with progressive glomerular and tubulointerstitial damage in the megalin-deficient group (22, 51) so that parameters related to sodium handling were comparable among groups.
With the bulk of sodium reabsorption occurring in the proximal tubule, this segment has been considered to efficiently contribute to volume retention as reported from a rat study with experimental nephrosis (7). Proximal tubular NHE3 is responsible for virtually all the local Na+/H+ exchange activity; it is the most effective proximal sodium transporter and furthermore plays a role in RME by its capacity to acidify endosomes for proper receptor-ligand dissociation (23). NHE3 activity can be estimated by its biosynthesis rate, its capacity to redistribute along the BBM microvilli (55), and its acidification capacity in BBM vesicles; the latter can also be used to measure its general activity (12). In our study, however, NHE3 failed to reveal major changes in these parameters except for the decrease in NHE3-specific BBM vesicular acidification in the GN groups. The decrease, however, appeared to be balanced by another, EIPA-resistant NHE isoform so that there was no general deficit in acidification of BBM vesicles. We therefore conclude that expression and activity of NHE3 were not megalin dependent and showed no changes with reduced RME. The drastic reduction of the proximal endosomal and recycling apparatus which otherwise influences NaPi-IIa retrieval (4) therefore does not seem to affect NHE3 expression.
The two other products of proximal BBM, NaPi-IIa and AQP1, showed similar results. Megalin deficiency per se caused a reduced retrieval of these products, leading to their increased abundance in BBM, which is consistent with earlier findings on NaPi-IIa expression (4). The GN groups showed decreased NaPi-IIa and AQP1 abundance and transcription rate, with immunoreactive signals equally reduced in megalin-positive and megalin-deficient proximal epithelia in the megalin-deficient group. These results partly agree with previous work in models of nephrotic syndrome or anti-Thy1 GN (22, 33), although the reason for the decreases remains to be established. Functionally, the decrease in AQP1 and consequent reduction in proximal transcellular water passage may activate tubuloglomerular feedback, which prevents inadequate water and NaCl delivery to the distal nephron in AQP1-deficient mice (45). Feedback activation in nephrosis may be relevant to stabilize sodium and water delivery to the postmacula segments (29).
Taken together, neither the abundance nor the activity of NHE3, NaPi-IIa, and AQP1 in our model is consistent with proximal tubule-based volume retention in GN and is thus at variance with data raised in puromycin aminonucleoside nephrosis (7). Along the same line, earlier micropuncture studies in rats were not supporting a direct role for the proximal tubule in sodium retention. Studies on experimental anti-GBM nephritis demonstrated equal fractional reabsorption of the proximal tubule in controls and anti-GBM treated rats (46), and rats with unilateral nephrosis showed reduced sodium reabsorption in proximal convoluted tubule and thick ascending limb (29). The absence of changes in juxtaglomerular COX-2 (50) and RAAS parameters further underlines our conclusion and is partly consistent with previous work (8, 16, 22, 46).
The most striking results of this study are related to the collecting duct system. In megalin-deficient controls as well as in both GN groups alike, the plasma membrane abundances of α-ENaC and γ-ENaC revealed by Western blotting (sum of band densities) and immunohistochemistry were enhanced, suggesting that despite the major quantitative differences in urinary protein levels, decreased proximal RME and resulting tubular proteinuria as well as glomerular proteinuria were both producing similar changes in the adluminal collecting duct cell compartment. The reason for these trafficking events in the absence of RAAS changes is not clear. In nephrotic syndrome, an influence of altered tubular protein composition on the intracellular trafficking machinery in the absence of mineralocorticoid changes has been considered (3). The absence of changes in β-ENaC parallels other work on disproportionate changes of ENaC subunits in nephrosis, suggesting that β-ENaC is less involved in regulatory events (22, 32, 39).
The increased Western blot abundances of ENaC subunits in the membrane fractions were characterized by selective enhancements of the smaller 55- and 30-kDa bands for the α-subunit and 70 kDa for the γ-subunit in the cortex of megalin-deficient mice, whereas the full-length bands were not altered. These increases were more pronounced in the GN groups, chiefly for α-ENaC, which also showed enhanced medullary signals of the smaller bands. RME deficiency produced additive changes. Increases in the 70-kDa γ-ENaC bands in GN were restricted to the cortex and also revealed changes in a second 55-kDa band; both bands showed no further changes with reduced RME.
These results may be important in light of recent findings suggesting that ENaC electrophoretic shifts occur upon enzymatic cleavage of its subunits and that these changes are consistent with an activation of the channel (5, 25, 28, 44). This mechanism has been illustrated by the observation that amiloride-sensitive sodium currents are increased in response to extracellular serine protease (52), and exogenously added trypsin and resident proteases had similar effects (5, 25, 28). Inhibitory tracts of ENaC subunits, that can be liberated by proteolysis, have further been identified (13). Proteases such as plasmin, which could be obtained from the urine of nephrotic rats, were shown to activate ENaC (43, 48). Plasmin may be locally activated from filtered plasminogen and appears to be particularly effective in full-channel activation by proteolytic processing of γ-ENaC (43). Passage of plasma proteases through the leaky glomerular filter in GN has been established, so that a similar mechanism of ENaC proteolysis and activation may be operative in our GN model. In the rat, we previously had obtained similar results in anti-Thy1 GN, with α-ENaC revealing an additional 65-kDa band typical of furin cleavage (22, 28). While the size of the present 70-kDa γ-ENaC cleavage product corresponds to published evidence (43), increases in α-ENaC bands were ranging at somewhat divergent molecular weight ranges compared with previous data. The larger 55-kDa band may be regarded as mildly divergent but analogous to otherwise shown 60- to 65-kDa ranges (22). The 30-kDa band suggests that the minor NH2-terminal extracellular domain (and not the long 60-kDa major portion) of the cleaved protein was recognized, since our antibody was directed to an NH2-terminal fusion protein and not to the COOH-terminally tagged recognition sites used by other groups; this agrees with previous data obtained with the NH2-terminally directed antibody which recognized the 30-kDa band as well (19, 21, 44). The 30-kDa fragment also reflects furin (or other protease)-dependent cleavage under stimulated condition in a heterologous system (28). It may further be considered that the occurrence of the 55-kDa ranges for α- and γ-ENaC products could be related to immature or degraded glycosylation, possibly resulting from insufficient intracellular processing, or via the action of filtered glycosidases or locally activated deglycosylation (19). The concept of ENaC activation by proteolytic cleavage is thus indirectly supported by our results in GN-associated proteinuria. Loss of proximal RME per se was associated with marked increases in ENaC cleavage products, suggesting that the relevant proteases are ligands of megalin. Data further support, at least for α-ENaC, that depending on the extent of proteinuria and ensuing proximal saturation of RME, megalin may thus indirectly affect downstream sodium handling.
Our data thus support a role of the collecting duct system as the crucial site in the nephron for sodium retention in proteinuric nephropathy which agrees with earlier work (11, 29, 32, 33, 38, 46). Apart from the presumed role for ENaC, primary, aldosterone-independent involvement of NKA in distal sodium retention has further been discussed (7, 38). It must, however, be considered that an ENaC-mediated rise in intracellular sodium concentration may also per se adjust the activity of NKA (9). The observed immunohistochemical upregulation of collecting duct α-NKA would therefore agree with activated transport secondary to a rise in ENaC-mediated intracellular sodium concentration (38).
The marked decreases in AQP2 abundance in the GN groups also reflect earlier data from models of the nephrotic syndrome (20) and could represent a physiologically appropriate response to extracellular volume expansion. AQP2 may act independently of plasma vasopressin levels, which tend to be normal or elevated in these models (2, 19).
Since lipiduria is a frequent phenomenon in proteinuric disease and plasma cholesterol was enhanced in our model, altered membrane lipids may further be related to the observed changes. Cellular fatty acids may cause cell dysfunction, and mice loaded with fatty acid and BSA exhibited systemic water retention (31) so that altered transepithelial reabsorption of sodium and water in the present model may be related to these changes. Since NaPi-IIa, NHE3, and aquaporins have been shown to be lipid raft associated, trafficking or membrane association of these proteins may be impaired in an altered cholesterol environment (10, 14, 30, 37, 41, 56). This may apply as well to distal epithelia, where NKA (53), NKCC2 (54), and ENaC (26) were shown to be partially lipid raft dependent, albeit with heterogenous functional consequences which are not entirely understood at present.
In sum, our study has identified renal changes in anti-GBM nephritis which may be relevant for volume retention, hypertension, and edema formation in human GN or nephritic syndrome. Our model suggests that in GN, altered filtrate composition as well as proximal tubular RME, controlled by megalin, are major determinants for renal-based salt retention and volume expansion. Whereas proximal tubular membrane transporters do not seem to be causally involved herein, structural changes in α- and γ-ENaC subunits, probably caused by proteolytic cleavage via filtered plasma proteases, as well as their enhanced membrane abundances may determine collecting duct-based sodium retention. Although tubular and glomerular proteinuria may produce analogous effects on sodium handling within distal epithelia, the effects of glomerular proteinuria clearly dominated those of blunted proximal endocytosis in contributing to ENaC cleavage and antinatriuresis.
This work was supported by the Deutsche Forschungsgemeinschaft (FOR 667; S. Bachmann, F. Theilig), the Swiss National Science Foundation (31-109677/1; C. A. Wagner), and the FP6 EU-project EuReGene (C. A. Wagner).
We thank Kerstin Riskowsky (FOR 667, Berlin) for technical expertise, Thomas Willnow (MDC, Berlin) for the gift of the megalin-deficient mice, and Michel LeHir (Zurich, Switzerland) for the gift of anti-GBM antibody and for scientific advice in treating the mice.
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2009 the American Physiological Society


text:
Mars. Most of these areas are low-lying to the surrounding, which allow copious amounts of the ever-present Martian dust to settle and collect there. This process builds up into stretches of soft and slippery sand. These areas are dangerous to cross due to the potential for hidden dangers under the sand, not to mention the danger of a sudden wind shift that could blow a dune over on top of a miner.
Dunes have proven to offer no substantial ores or other reclaimable valuables within their ever shifting areas. Fortunately for the average miner, the Martian landscape is so varied as to normally prevent long runs of Dunes from forming.
|Weapons | Defense | Intelligence | Security | Radar
|In-game Elements
|Homestead | Settlements | Outposts | Volcanoes | Ruins | Craters | Plains | Dunes
|In-game Locations
|Earth | Mars
|Rules and Regulations
|Basics | Payouts | Special Prizes | Purchases | Alliances
|Top Ten Players
|slyfox | lomaxmd | eddymac | brutal | faxfox | RickDN | Algonquin723 | nicennaughty | Hitman | melanielv73
|Official Website
|Official site


text:
An Annual Conference on the Future of Content in Digital Media
The program for the conference has been organized to be informative, wide-ranging and provocative. A major focus will be on the Heritage Minister's pending consultations on strengthening Canadian content creation, discovery and export in a digital world. Keynote speakers will also address technological disruption in the digital media sector, how to find and measure digital content, consumption and dollars, and the development of creative clusters. Guest speakers include key figures involved in Canada's digital startups and industry experts focused on digital public policy.
For Ontario lawyers, this program contains 7.0 Substantive CPD hours
Welcome and Introduction: Don McLean, Dean, Faculty of Music, University of Toronto
Keynote Address: "Cultural Strategy in a Time of Rapid Change"
Charles Falzon, Dean, Faculty of Communications and Design, Ryerson University
The Faculty of Communication and Design (FCAD) at Ryerson is home to some of Canada's most innovative schools in media and creative industries. Charles Falzon is not only the Dean of FCAD but also a thirty year media industry veteran. He is also a member of the Department of Canadian Heritage's Expert Advisory Group on the future of Canadian content. In this keynote address, Charles brings together these three perspectives to discuss the responsibility, the challenges and the value of public policy and cultural strategy in a time of rapid change.
Exploring Policy Options in the Audiovisual Digital World
This panel is a follow-up to the keynote address and will feature a debate on the Minister's consultations. Will the borderless nature of the Internet defeat any attempts to support Canadian content other than through subsidy? Should we loosen the rules for Cancon in order to seek global markets? What are the opportunities and pitfalls?
Grant Buchanan, Partner, McCarthy Tétrault LLP, Toronto
John Anderson, Principal, Anderson Consulting, Ottawa; Author of "An Over-the-Top Exemption" study for the Canadian Centre for Policy Alternatives
Valerie Creighton, President and CEO, Canada Media Fund
Reynolds Mastin, President and CEO, Canadian Media Producers Association
Refreshment Break
Technological Disruption in the Digital Media Sector
With technological change, we may see new approaches to global problems, including the digital divide, the gender gap and the changing nature of jobs. Entrepreneurs in the digital space discuss these changes in the Canadian context.
Kenneth Rogers, Associate Dean of Research at the School of the Arts, Media, Performance, and Design at York University
Vlad Dascalu, Partner, Head of Strategy, Pinch VR
Tammy Johns, Co-Founder, Skills.com
Allen Lau, Co-founder, Wattpad
Buffet Luncheon (provided)
Luncheon Address: "Strengthening Canadian Content Creation, Discovery and Export"
Julia Johnston, Digital Media Entrepreneur, Toronto
The Minister of Canadian Heritage's consultation on the future of Canadian content in a digital world is now well launched across the country. In this luncheon address, one of Canada's most experienced digital entrepreneurs provides a unique perspective on what should be done to strengthen Canadian content in the system. Julia Johnston has seen it from all sides – as a communications lawyer, as a business advisor, as a media executive, as a chief operating officer, and as the co-founder of two internet companies. She will provide a personal view as to what she would do if she was the Minister of Canadian Heritage.
How to Find and Measure Digital Content, Consumption and Dollars
This panel addresses three central problems for digital content: How do you discover it? How do you measure its consumption? And how can you derive revenue from it? While the initial focus of the discussion will be on measuring music consumption, the panel will also address the general problem of discoverability, and the measurement of consumption and revenue for video content, particularly Canadian content.
Catherine Moore, Adjunct Professor of Music Technology & Digital Media, Faculty of Music, University of Toronto
Jim Lidestri, CEO, BuzzAngle Music, New York
Barbara Motzney, Chief Consumer Officer and Executive Director, Consumer Affairs and Strategic Policy, CRTC
Rachel Noonan, Entertainment & Media Specialist
Refreshment Break
Creative Clusters: Can Canada Compete?
Many economists have focused on the concept of "creative clusters". Canada has such clusters in Toronto, Vancouver and Montreal. But all too often the components of the cluster (music, film/TV, books, games) operate in silos that never talk to each other. This panel examines the links between the sectors and the need and role for umbrella organizations to coordinate policy.
Trina McQueen, Adjunct Professor, MBA Program in Arts and Media Administration, Schulich School of Business, York University
Sara Diamond, President, OCAD University
Peter Lyman, Senior Partner, Nordicity Group Limited
Chris Unwin, Founder and Executive Director, Free: A Creator Studio, Toronto
David Wolfe, Co-Director, Innovation Policy Lab, Munk School of Global Affairs, University of Toronto
Closing Wrapup: Don McLean, Dean, Faculty of Music, University of Toronto
Termination of Program


text:
Lowriding is part of Chicano (Mexican-American) culture. Lowrider's creed is to tour as slowly as possible, their axiom is "Low and Slow". Lowrider bikes are one of the most fabulous and distinctive invention of this culture. This type of bikes are a cross between the Harley and the cruiser bike, custom upholstery, sound systems, rims, and special whitewall tires are common on this mode of transport. Despite all that customizations and styling, Lowriders are well known to be one of the most comfortable of bikes. Here now the Lowrider Bike wallpapers that shows breathtaking example of technical and artistic ingenuity. Enjoy these showcase of personalize piece of arts on wheels
5 thoughts on "19 Incredible Lowrider Bike Concept for Your Desktop"
I am liking these bikes I have just stumbled this It reminds me of a post I once posted on concept bike, I am notorious for stealing these sorts of pictures and blogging about them , great post. But I am not that keen on the skeletal ones.
It reminds me of a discovery program about building bikes – interesting stuff, and beautiful canvas art photos. Thanks for sharing.
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text:
Clinical Trial Liability Lawsuits
Product ID: 703645
- Duration: 60 Min
Customer Care
Fax: +1-650-565-8542
Email: email@example.com
This hour-long session will provide insights on liabilities arising due to non-compliance and oversight in clinical trials. The clinical affairs expert will also discuss identifying the types of litigation and how best to eliminate the source of risk with real world examples. Participants will learn to appreciate the importance of formal training in conducting proficient clinical trials.
Why Should You Attend:
There are several types of injury that can arise in clinical trials that sponsors, investigators and staff should be aware of so that they can avoid them. Understanding the sources of risk, such as possible conflicts of interest, patient eligibility/ineligibility, informed consent and protecting patient dignity can held avoid or minimize the likelihood of errors or omissions resulting in clinical trial protocol violations and liability.
Investigators are at risk of litigation from errors of the study sponsor; at the same time, sponsors are at risk of litigation from mistakes or oversight by investigators or their staff. To protect each party from liability created by the other, a mutual indemnification by the sponsor and the institution is generally included in the clinical trial agreement.
This presentation will:
- Help you distinguish between the types of litigation in clinical trial liability lawsuits
- Identify and avoid sources of risk that can bring liability
- Gain appreciation of the importance of formal training for conducting clinical trials
- Discuss clinical trial liability insurance to ensure protection
Safeguarding trial subjects is a key priority and awareness of clinical trial liability can help preserve their rights and avoid harm. This webinar will provide inputs to protect sponsors, investigators and their staff from the cost of defending lawsuits that result from the fault of the other party.
Areas Covered in the Webinar:
- Types of litigation
- Sources of risk
- Legal duties of clinical trial sponsors
- Inventory control and defective devices
- Examples of actions considered to be fraud
- Examples of clinical trial liability lawsuits
- Insurance to provide protection
Who Will Benefit:
- Healthcare providers interested in conducting clinical research
- New clinical research coordinators
- New principal investigators
- Administrative managers in charge of clinical research
- Regulatory compliance associates and managers
Jonathan McDermed has more than 30 years of experience in oncology, clinical research, product development, regulatory/clinical affairs and marketing in the pharmaceutical, medical device and in vitro diagnostics industries. He is a PharmD graduate of the University of Southern California and was an associate professor of clinical pharmacy at USC, assigned at the USC/Norris Comprehensive Cancer Center in Los Angeles, CA, where he was an investigator and co-investigator of Phase I-II studies of novel serotonin (5-HT3) receptor antagonists for preventing chemotherapy-induced nausea and vomiting.
He is recognized as a subject matter expert in prostate cancer diagnosis, prognosis and treatment. He was instrumental in the development and r510(k) clearance of a novel, immuno-PCR assay for quantitating prostate cancer specific antigen (PSA) serum levels in men following prostatectomy and the NADiA ProsVue PSA slope assay for identifying men at a reduced risk of clinical recurrence. He has published numerous abstracts and peer-reviewed manuscripts throughout his career and presented the results of his research studies in the US and in international oncology and urologic oncology symposia.
Topic Background:
Research, by definition, involves risks. It is up to everyone involved – sponsor, investigator, and clinical research coordinators to try to minimize those risks for subjects. Failure to do so often prompts legal action. The targets of litigation are typically investigators and clinical institutions. However, sponsors, institutional review boards (IRBs)/research ethics committees (RECs), and even patent holders of medical devices are increasingly being named in lawsuits. Because the number of malpractice lawsuits is increasing and the amount of awards has skyrocketed, it is important that investigators recognize the sources of risk and how to protect themselves through careful and ethical conduct of clinical trials.
Refund Policy
Registrants may cancel up to two working days prior to the course start date and will receive a letter of credit to be used towards a future course up to one year from date of issuance. ComplianceOnline would process/provide refund if the Live Webinar has been cancelled. The attendee could choose between the recorded version of the webinar or refund for any cancelled webinar. Refunds will not be given to participants who do not show up for the webinar. On-Demand Recordings can be requested in exchange.
Webinar may be cancelled due to lack of enrolment or unavoidable factors. Registrants will be notified 24hours in advance if a cancellation occurs. Substitutions can happen any time.
If you have any concern about the content of the webinar and not satisfied please contact us at below email or by call mentioning your feedback for resolution of the matter.
We respect feedback/opinions of our customers which enables us to improve our products and services. To contact us please email email@example.com call +1-888-717-2436 (Toll Free).
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text:
Range has received government and regulatory approvals for the implementation of the Beach Marcelle waterflood programme. Separate work programme stages will require additional approvals as the project progresses.
The Beach Marcelle waterflood is the largest scale waterflood project to be undertaken in recent times in Trinidad and is expected to be a major contributor to the Company's production growth. The majority of Range's 1P reserve base is found in the Beach Marcelle block (14.7 mmbbls of 1P reserves).
Range Resources Limited
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Research for Art, Architecture & Design: Develop a Research Plan
Methods & Theories
Architectural Research Methods byISBN: 1118418514Publication Date: 2013
Methods and Theories of Art History byISBN: 9781856698993Publication Date: 2012
Thinking about Architecture: an introduction to architectural theory byISBN: 9781856697552Publication Date: 2011
Develop a research plan
Develop your research topic.
What are the key concepts of your topic? Can you broaden or narrow your focus? Consider focusing on one or more of the following:
- time period
- place
- specific event
- specific people
Does your topic overlap other subject areas such as anthropology, geography or political science?
Think of 2- 3 questions that you will need to explore.
- Think about the history of your topic, and its categories.
- Who are the key people? What did they do? Why did it happen?
Under each question, think about the following aspects:
- What do you already know about your topic or issue?
- What do you need to learn to better understand your topic or issue?
- What kind of information resource might provide the answer to these questions?
Consider your audience.
- Who will read your paper?
- Why will it be of interest to them?
- What will be new to them?
Oral History
Oral History in the Visual Arts byISBN: 9780857851970Publication Date: 2013


text:
If the world almost extinct, you are the one and only savior, you must make this world 'back to normal' so you need heirs. There are only two girls left and you must marry one of them and make her your children's mother. And when you already choose, the other one will die in aliens' arms.
-The first girl is an ex-whore (why ex? Because there is no guy in this world except you so she can't continue her job).
-The second girl is an old-fashioned (you already know that it's includes: Religious, book-lover, introvert, and such).
Which one do you choose?
Most Helpful Guy
I'd choose the second. Not cuz what the other is or isn't but the other would have more appeal to me. I would bet on her being a better mother too which would be very important in a situation like this. All that was right/wrong, pc/not pc, fair/not fair wouldn't matter anymore. I need a mother to bear and help raise children. I'd pick the one that has showed to be a better judge in life and didn't become a prostitute. Not that the prostitute wasn't or couldn't be a good person but those are the type of decisions people would be faced with in an end of the world situation.11|00|0
But... Who knows that the whore will be a better mother than the old-fashioned one?
Of course that's possible but on a gamble like that, I'd choose the old fashioned one. The other has proven she's prone to very bad decisions.
Okay then. Hahaha xD


text:
Khloé Kardashian: I was told I was too big to work with
Khloé Kardashian was told by stylists she was too big to work with.
The 'Keeping Up With The Kardashians' star credits her long-term stylist Monica Rose for keeping her positive when others told her she was too fat.
She said: "I definitely think the fashion industry, and people in general, look at me more now that I've lost weight. Even on shoots, I would never have options for clothing. There would always be this attention on Kourtney and Kim, but I was too much work for [stylists] or they had nothing in my size. I wasn't even that crazy big!
"At my fattest, Monica would always come with racks of clothes and make me feel special. She never told me, 'Oh, they don't have that in your size.' Other people actually said, 'I just can't work with you' - because I was too big. That always hurt my feelings, of course. [Now to those people] I'm just like, 'F**k you. I'm not going to reward your bad behaviour.'"
And the 31-year-old television personality went on to praise Olivier Rousteing at Balmain and opened up about their "authentic and organic" friendship.
She added: "We love Olivier, we love Balmain. It was all very authentic and organic how my sisters and I built a relationship with him, we were just fans of his work."
Despite being much more confident now, Khloé admits she still feels insecure at times.
She told HarpersBAZAAR.com: "I like to say I don't care what people say about me, but that's not true. Not wanting to go to certain places because I wonder what will be written about me the next day - I hate that I carry that with me.
"What's sad is that I can read 10 beautiful comments [on Instagram] and then one bad one and I'm like, 'Ugh!'"


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The Sarbanes-Oxley Act is presenting chief financial officers (CFOs) with significant challenges beyond the well-publicized executive certification and internal control requirements, according to a study released by Protiviti Inc., a leading internal audit and business and technology risk consulting firm.
Although efforts to comply with Sections 302 (executive certification) and 404 (internal control reporting) were cited by one in three CFOs (34 percent) as the most challenging aspects of Sarbanes-Oxley, following closely behind in the poll were:
- aligning audit committee activities with legal and regulatory requirements (27 percent);
- and recruiting an audit committee "financial expert" (23 percent) as defined by the U.S. Securities and Exchange Commission (SEC).
"Publicly traded firms remain under intense pressure from boards and shareholders to meet the new governance requirements of Sarbanes-Oxley, the SEC, the exchanges and other regulatory bodies," said Everett Gibbs, managing director for Protiviti. "Companies are undergoing significant operational and cultural changes needed to achieve compliance, and our study indicates these changes are occurring well beyond executive certifications and internal control issues. Directors and management are addressing areas such as audit committee oversight, board composition and structure, and auditor independence."
Among the survey's other key findings:
Changing Board Practices:
With an increased focus throughout businesses and the media on independent directors, 40 percent of CFOs overall, and 33 percent of CFOs at large companies, report there have been or will likely be changes in the makeup of their boards or board committees in response to the Act.
Reasons cited for board changes included new rules concerning independence (41 percent) and the desire for greater levels and diversity of experience on the board (25 percent). The survey results indicate that another significant change occurring at the board level is the type of information directors are receiving. In 83 percent of firms, management decided on its own to provide more information to the board and its committees, while in one of three companies directors issued requests for more detailed information.
Also of note, 60 percent of CFOs polled said that since the passage of Sarbanes-Oxley, their boards have expanded their oversight of the external audit process as well as internal audit activities. In addition, 46 percent of respondents with large companies report their directors or board committees are conducting self-assessments in which they are rating their own performance and reporting the results to the board. Forming disclosure committees
Almost half of all companies surveyed (49 percent) have formed a disclosure committee, as recommended by the SEC. According to the poll, nearly all of these companies are relying on their disclosure committees to oversee the executive certification process on an ongoing basis.
The survey findings also confirm that large businesses are more likely than small firms to have disclosure committees in place. Seventy-four percent of large companies have formed a disclosure committee compared to just 33 percent of small companies, according to the results.
Preserving Auditor Independence:
Companies are taking definitive actions with regard to preserving the independence of the external audit function. Nearly three out of four audit committees -- 72 percent -- have adopted procedures governing non-audit services rendered by external auditors (including 83 percent of large firms).
Within this group, more than half not only have procedures that parallel the SEC's requirements, but they also mandate that specific processes be followed for gaining approval of any non-audit work performed by the external auditor.
Furthermore, nearly 13 percent of large companies completely prohibit their external auditors from performing non-audit services.
Estimating the Cost of Sarbanes-Oxley Compliance:
Many of the survey's respondents reported it is still too early to know the final cost to achieve Sarbanes-Oxley compliance.
CFOs surveyed offered projections ranging from $10,000 to well over $1 million, while 12 percent did not offer an estimate, stating they did not know what the costs would be. Extrapolating the survey data, the total compliance costs for all public companies combined are projected to run to several billion dollars.
Assessing the overall survey, Gibbs said, "The results show momentum is building. Corporate governance reforms are influencing behavior, as evidenced by CFOs and public companies acting on a wide array of issues that continue to reshape the governance landscape.
Companies have clearly begun the journey; however, the verdict is still out as to how long it will take for these steps to restore investor confidence. "Insights on Today's Sarbanes-Oxley and Corporate Governance Challenges is available for download at http://www.protiviti.com
Protiviti is a leading provider of independent internal audit and business and technology risk consulting services. Protiviti, which has more than 30 locations in the United States, Europe and Asia, is a wholly owned subsidiary of Robert Half International Inc. (NYSE symbol: RHI). Founded in 1948, Robert Half International is a member of the S&P 500 index.


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The Big Score (Subtle)
| Headshots
| Get 20 headshots
| Mission Time
| Complete within 16:00
| Cha-Ching
| Drop the gold in the train within :30 seconds
| Accuracy
| Finish with a shooting accuracy of 60%
If you decide on the first approach (choice A 'The Subtle Approach) for the heist, you'll be presented with two missions:
- Stingers: Steal a Cop van
- Gauntlet: Steal 3 Bravado vehicles, mod them, and deliver them to the hideout
This approach is a little easier than choice B 'The Obvious Approach' but you will need to hire an extra crew member, so get ready!
For choice A ('The Subtle Approach') you will need; 2 gunmen, 2 drivers and 1 hacker.
Lester Explains; The first gunman comes into the bank with you, helps you move the gold into the reinforced hot rods (the Bravado's you modded).
Lester Explains; The second gunman is there to take down the armoured cars and bring the hostages to a safehouse.
For choice B ('The Obvious Approach') you will need; 2 gunmen and 2 drivers.
Top Wiki Contributors
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text:
At a Senate Budget Committee hearing on Thursday, Sessions, the panel's ranking member, said the government didn't have the money for rail projects. But Whitehouse pressed the Alabama senator to keep an open mind on the idea, likening high-speed rail to the development of highways.
"You'd find that it was actually worth spending that money because it carried follow-on economic effects that were worth far more than it," Whitehouse said.
In the end, Whitehouse might have made some progress with Sessions — but appears to still have quite a ways to go.
"I recognize the Northeast could justify it more than a lot of places I see it's being projected to go," Sessions said to Whitehouse.
"That's all I needed to hear," Whitehouse said, to scattered laughter in the hearing room.
The exchange came after Sessions asked witnesses whether federal policy had encouraged states to spend money they might have otherwise kept in the coffers.
Chris Edwards of the libertarian Cato Institute then brought up high-speed rail, which the Obama administration has made a priority. According to Edwards, the federal government may help out with the construction for high-speed rail, but not the operating cost.
"These cities and states are going to be left down the road with these, you know, very fancy new systems when bus systems would have been cheaper, with lower operating costs, and now they're stuck with all this expensive infrastructure," Edwards said.


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Death Note - Requests for new threads
Since the Death Note forum is now limited (only Mods and Admin can create new threads), this thread serves as a way to request a new thread to be created.
Please post here if you wish to request a new Death Note thread. Please bear in mind that we will only accept reasonable requests, we wish to maintain the focus within the forum and cut down on the noise (so no pointless threads cluttering up the place).
Don't forget that you may still post in existing threads as usual, the only restriction is that you may not create new threads within this particular forum.
How 'bout we put a request thread for avies and siggies? You know, for people who want one?
How about a "Death Note" thread...
A thread were people would post victims and humoros ways of death...
Ex: "George Bush, death by killer terorist lolis"
I wouldnt mind seeing a poll asking if any of us found the death note and knew what it could do would we use it
However, most likely, the Mods won't allow such a poll because :
I only watched the anime so far (ep. 3) and thought about an interesting aspect: Since you can write down times, you can write dates as well, right? why doesnt Light write his own name and let the death be on his 111th birthday cause of sugar-shock or something? then the shinigami-eye-trade-thingy wouldnt be a thread anymore, right? or if the timespan would be halfed just say 200th birthday... even then he has enough time to live. and he can be assured, that he wont get the death-sentence, since he allready knows how he dies. any failures in this thought? discuss!
(can you guys please make my own thread with this? thx)
Actually, merely understanding the nature of the shinigami eyes proves that it isn't possible. I haven't seen episode 3 yet and I don't remember how much is told how quickly, so in case something about the shinigami's eyes wasn't revealed in episode 3, I'm putting the reason in a spoiler tag:
And anyway, there really isn't enough to discuss about it, to be worth an entire thread of discussion.
Hi admin, please make a thread call " There are no Good or evil, Right or wrong in Death Note" or something like that. Then I will post my opinion in that forum :).
Thank you.
Actually I've been thinking about the need for a thread like this. I'll go ahead and create that thread already. I'm just a little worried about the possibility of this thread bursting into flame.
Yo NoSanninWa, thx for making such a post ^^. u see even in episode thread or manga thread. People are talking about moral issue, so such a thread is a "neeD".
Just a suggestion, I want a thread discuss about the author
小畑健 - who draw the manga
大場鶇- Also a manga and also he is the original author who create this Death note's story, but no one know his real "identity" Which I found quite interesting. L was a character which base on himself. And many rumors...
I saw this on another forum, but it called for a lot of interesting convo.
The thread was a debating the philosophies of both Light and L, about whether or not criminals should be killed? Who's philosphy was right?
It's another morals thread, but I think that it might bring up a good debate among the posters.
Just thought I suggest a thread.
Is it alright to have a thread for manga readers to talk about the anime.. as in future plot, and next episode talk? It could be labeled with a huge spoiler if you want. If its not allowed - then i understand.
If you are not familiar with this, we have two threads in the Kanon forum
Kanon - Spoilers & Speculation (for experienced Kanonites only)I take it you are asking for a Death Note Spoilers & Speculation thread for those who have read the manga and want to discuss how the anime may or may not follow the manga along with personal theories and such like. Clearly not for the anime-only viewers since it would contain unmarked spoilers.
And if required, we could have a separate Death Note Speculation & Theories thread for the anime-only viewers where no spoilers are allowed.
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text:
Acid Test Graduation Ceremony 1966 (Actual Footage) MP3
Actual footage of Ken Kesey and the Merry Pranksters' Acid Test Graduation ceremony filmed in 1966.
Acid Test: The Global Challenge of Ocean Acidification MP3
This groundbreaking NRDC documentary explores the startling phenomenon of ocean acidification, which may soon challenge marine life on a scale not seen ...
Recondite - Tie In (Acid Test) MP3
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Ken Kesey Talks About The Meaning of the Acid Tests MP3
The acid test parties began after Kesey's experience with mind-altering drugs as a volunteer test subject for Army experiments in 1960 (later revealed to be part ...
Jerry Garcia on The Acid Tests | Blank on Blank | PBS Digital Studios MP3
"When we fell in with the Acid Tests we a started having the most fun we had ever had." - Jerry Garcia Interview by Joe Smith May 23, 1988 Cassette Tape ...
army acid test MP3
this is a national archives and records administration film file of the army testing lsd on servicemen.
The Chemical Brothers - The Test (2003 - Remaster) MP3
Download on iTunes: http://smarturl.it/chembrosfurther Follow The Chemical Brothers Official Site: http://www.thechemicalbrothers.com Facebook: ...
Quick and easy Silver test - WARNING: if you are under 18 or unfamiliar at all with these testing methods, seek the help of a Professional -do not try this yourself.
The Acid Tests (1966) audio "PART 2" MP3
The Acid Test Reels "Part 2" of the acid tests recorded in 1966 included the merry pranksters, ken kesey, grateful dead *COMMENT, RATE, SUBSCRIBE*
The Acid Tests (1966) audio MP3
The Acid Test Reels audio of the acid tests taken in the 1966, includes the merry pranksters and the grateful dead **COMMENT, RATE, SUBSCRIBE*
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Necropsycho - Acid Test (album: VA Apocalyptic Ritual) 2011.
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Blackout Youtube Selection, Bringing you the finest and latest of the heavier styles of Drum & Bass! Out now!
Psy Trance Night FullOn GOA 2014 \ Acid Test \ Twilight Midnight Edition MP3
TracksPlaylisten: 1.Drip Drop - Land of Bubble 2.R2 Unit & Dj Yamaga - Slowly But Surely 3.Niria - 15000 Micrograms...
Tilt Acid Test and Center the Swing Drills Shawn Clement Wisdom In Golf MP3
http://www.wisdomingolf.com Get his Amazing 2013 video series filmed and edited by the Art Institute of Pittsburgh at the Harvest Hill Golf Club in Buffalo New ...
Jerry Garcia - Acid Test 1967 Interview MP3
Jerry Garcia & Co. at 710 Ashbury in Spring 1967 Interview. 83min. The interview came to me through FM radio broadcasts in the early-to mid-nineties.
Taken - A Sci-Fi Original Entire Episode - Acid Test (Episode 4) MP3
This Video Is For Educational Purposes.
Quick Ratio (Acid Test Ratio) MP3
This video demonstrates how to calculate and interpret the Quick Ratio (aka Acid Test Ratio). An example is provided to show how the Quick Ratio can be used ...
Quick (Acid Test) Ratio Analysis in 15 minutes - Financial Ratio Analysis Tutorial MP3
Clicked here http://www.MBAbullshit.com/ and OMG wow! I'm SHOCKED how easy.. No wonder others goin crazy sharing this??? Share it with your other friends ...
Acid Test Ken Kesey And The Merry Band of Pranksters MP3
Space Tribe & Electric Universe - The Acid Test MP3
Artist: Space Tribe & Electric Universe (together as ESP) Release: Collaborations Label: Space Tribe Music Year: 2004 Genre: Psytrance.
pause- can you pass the acid test \õ/ \õ/ MP3
amantes do psy:http://www.orkut.com.br/Main#Community?cmm=86214681.
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How to tell if your silver is real or not. Sorry for the breaks, but Youtube only allowed 15 minutes max.
Low Stomach Acid Test MP3
FOLLOW MY BLOG @ http://shanespilltalk.blogspot.ca/ Low Stomach Acid Test Want to figure out what is causing you stomach discomfort? Try this low stomach ...
ESP - Acid Test (2015 Remix) MP3
Subscribe: http://bit.ly/SubscribeDacruRecords Buy at it: https://pro.beatport.com/release/intricate-web/1517594 Electric Universe and Space Tribe team up ...
Sony Xperia Z - Acid Test MP3
We dip a Sony Xperia Z into a bath of sulphuric acid to see if it can handle more than water. Happy April 1st!Subscribe now: http://smarturl.it/StuffTV Welcome to ...
Sub Focus - Acid Test MP3
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Acid Test - 14k Scrap Gold Jewelry MP3
Demonstration of the Acid Test used to verify the gold authenticity of scrap jewelry before buying from the public.
Meat Beat Manifesto - Acid Test MP3
Test your cerebral hemispheres with this. Music/video by Jack Dangers.
deedat at his best part 13 ( Acid Test Must Watch) MP3
Acid Test by Ahmed Deedat deedat at his best part 13 Acid Test ( Must Watch) Contradiction in Bible.


text:
"We've initiated a Company wide cost reduction program to align our expense levels with our revenue expectations for 2013. This will reduce our current spending by approximately $1.7 million per year as well as an additional $1.3 million of previously planned expenditures which will not be made," said John Gandolfo, Chief Financial Officer.EBITDA EBITDA for 2012 improved to a loss of $2.6 million, compared to a loss of $3.0 million in 2011. Mr. Gandolfo further commented, "If we are successful in achieving the previously given 2013 revenue guidance of $38 to $40 million, as well as the expense reductions referred to above, we believe we would generate positive operating income as well as positive EBITDA for the year." Financial Liquidity Cash and cash equivalents and net accounts receivable, was $12.1 million at December 31, 2012, compared to $7.8 million at December 31, 2011. On August 24, 2012, the Company entered into a Credit Agreement with an entity managed by OrbiMed, whereby OrbiMed agreed to provide an initial $20 million term loan and Bacterin may also borrow an additional approximately $5 million upon achievement of certain revenue objectives prior to December 31, 2013. Approximately half of the proceeds of this credit facility were used to repay existing debt and expenses related to the transaction. Conference Call Details The company also announced that it will hold a conference hosted by Guy Cook, Chief Executive Officer, and John Gandolfo, Chief Financial Officer, to discuss its financial results at 10:00 a.m. ET, on Wednesday, March 27, 2013. Please refer to the information below for conference call dial-in information and webcast registration. Please call the conference telephone number 5-10 minutes prior to the start time. An operator will register your name and organization. If you have any difficulty connecting with the conference call, please contact The Cockrell Group at 1-877-889-1972.
|Conference dial-in:
|International dial-in:
|Conference Name:
|Bacterin's 2012 Fourth Quarter & Year End Results Conference Call
|Webcast Registration:
|Click Here


text:
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DAVID GREENE, host:
This is MORNING EDITION from NPR News. I'm David Greene.
RENEE MONTAGNE, host:
And I'm Renee Montagne. More trouble is brewing in the occupied West Bank. The long-standing acrimony between rival Palestinian factions Fatah and Hamas has turned violent. Fatah leaders say Hamas is plotting to take over the West Bank by force, just as it did in the Gaza Strip two years ago. NPR's Lourdes Garcia-Navarro reports.
LOURDES GARCIA-NAVARRO: Ahmed Shreen sits in lush garden in the West Bank city of Qalqilya. While the setting couldn't be more idyllic - birds flit from tree to tree, a light breeze blows - the Fatah representative here says darkly that Hamas militants are stockpiling weapons in the city.
Mr. AHMED SHREEN (Fatah): (Through translator) The Hamas leadership wants to take over the West Bank, and the proof is that we have discovered large weapons caches right here in Qalqilya in Hamas areas.
GARCIA-NAVARRO: In 2007, bloody clashes broke out between Hamas and Fatah in Gaza, ending in a major defeat for Fatah. Hamas took over the Gaza Strip, while Fatah retained control in the West Bank.
The two groups have fundamentally different views on how to deal with the Israeli occupation. Hamas — which is considered a terrorist organization by Israel, the U.S. and other Western countries — refuses to recognize Israel and insists on armed resistance. Fatah, which heads the Palestinian Authority, has acknowledged Israel's right to exist and has engaged in almost two decades of peace negotiations.
Currently, the two factions have held several rounds of talks in Cairo aimed at forming a unity government, but Fatah's Ahmed Shreen says a deal with Hamas is unlikely.
Mr. SHREEN: (Through translator) The possibility of a solution is very remote, even though we've put all our efforts into dialogue with Hamas. What I'm worried about is that while we are talking in Cairo, Hamas will be planning the coup in the West Bank.
GARCIA-NAVARRO: For now though, it's the Fatah-dominated security forces that have been cracking down on Hamas fighters in the West Bank. Over the past few weeks, Palestinian Authority security forces have fought two gun battles with Hamas militants in Qalqilya. In total, nine people have been killed, including four Hamas fighters and four Palestinian Authority policemen.
Qalqilya Mayor Wajia Qawais is a member of Hamas. One of his nephews was among the Hamas members killed in the battle here.
Mayor WAJIA QAWAIS (Qalqilya): (Through translator) Qalqilya is going through a very tense period, which has brought pain to our citizens. There are big and crucial differences between the different factions.
GARCIA-NAVARRO: In Gaza, Hamas spokesman Ihab Al-Ghussain alleges that Fatah is doing the bidding of the Israeli occupiers.
Mr. IHAB AL-GHUSSAIN (Hamas): (Through translator) They're continuously arresting our men in the West Bank. They committed an awful crime against a group who were fighting the Israelis for more than six years. The Israelis couldn't capture them, so the Palestinian security forces did the Israelis' dirty work and assassinated them.
GARCIA-NAVARRO: The Obama administration has called on the Palestinian Authority to crack down on militants and enforce the rule of law. Some analysts say that the renewed raids on Hamas fighters are an attempt by the P.A. to show that it is heeding that call, in advance of the possible resumption of peace talks with the Israelis.
(Soundbite of horn honking)
GARCIA-NAVARRO: On the streets of Qalqilya, there is fear. Few people wanted to talk publicly about the clashes. Residents say privately that they are worried that all-out war could break out between the two sides. One resident who consented to speak, Abu Ahmed, says that's a real concern here.
Mr. ABU AHMED: (Through translator) People are very angry about what happened. People do not want resistance fighters like those Hamas men to be killed. At the same time, people do not want chaos; we want the rule of law. The Hamas fighters are from our families. The Fatah policemen are from our families too.
GARCIA-NAVARRO: He says we want this power struggle to stop. The infighting, he says, only hurts the Palestinian people and helps the Israeli occupation.
At a nearby house, the mother of one of the slain Hamas fighters sits with other female relatives. Raba al-Qawais says her son was initially taught to be a fighter by members of Fatah.
Ms. RABA AL-QAWAIS: (Through translator) Fatah is the foundation of the resistance in Palestinian society. Now they have sold out and they have betrayed the young men who followed them. This is what happened to my son.
GARCIA-NAVARRO: She says she thought he'd die in battle with the Israelis, not at the hands of fellow Palestinians.
Lourdes Garcia-Navarro, NPR News, Qalqilya.
Copyright © 2009 NPR. All rights reserved. Visit our website terms of use and permissions pages at www.npr.org for further information.
NPR transcripts are created on a rush deadline by Verb8tm, Inc., an NPR contractor, and produced using a proprietary transcription process developed with NPR. This text may not be in its final form and may be updated or revised in the future. Accuracy and availability may vary. The authoritative record of NPR's programming is the audio record.


text:
Answered By: Carla Lillvik Last Updated: Jun 17, 2016 Views: 68
The Library uses the Reserves List Tool in Canvas to manage reserves. The IT Service Center will assist with technical questions about setting up your course reserve tool. Please contact the Course Reserves Librarian (firstname.lastname@example.com) with any additional questions about course reserve materials or processes.
*Please Note: Only books from the Gutman Library Collection may be placed on course reserve.
To request new books to be purchased by Gutman Library:
- Place requests as soon as possible - it can take up to six weeks for new books to arrive.
- Complete a new citation request in the Reserves List Tool.
- If the Reserves List Tool is not yet available or if there are any other issues, contact the Course Reserves Librarian (email@example.com) .
To request books previously on reserve at Gutman Library:
- Locate the citation in the Reserves List Tool and select it to resubmit for the upcoming semester.
To request books not previously on reserve but within the Gutman Library Collection:
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ISI Winter Classic
February 10–12, 2017
Goggin Ice Center is pleased to host the 2017 ISI Winter Classic Team Competition!
View the competition schedule. All Scheduling Questions can be directed to ISI Event Coordinator, Kim Hansen
You can now register for Individual and Team practice ice. View the registration page.
Select "Skating Programs on the left-hand side. All sessions are located under the "Winter Classic Practice Ice" tab.
You will be directed to set up an account for each skater in order to register for sessions.
If you have any questions, please contact Cassandra MacDonald
LaRosa's Pizzeria is the proud sponsoring restaurant for our competition. Located just minutes from the rink between Lynn Avenue and Locust Street. Contact LaRosa's Pizzeria for all your dining needs! 513-347-1111


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I know he loves me. He has not change any behaviors. He works out of town during the week and home on the weekend. He has text her some. While home. But while he is out of town the talk on the phone for hours at a time. He rarely calls me we text. He messages with her on FB . They have been friends for years. I keep telling myself there is nothing to worry about. I have been burned many times before. I am afraid of I don't trust. Him I k ow he will never gone. I know I can not tell him I have looked at the call logs. How do I trust him? He is wonderful to me. His actions word me have not changed except he doesn't answer my text when look at times it's because he is on the phone with her. HELP
I am becoming jelous of my boyfriend and a long time friend that is family.
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text: Hey guys. I made another video, this one is more of an advanced basic combo. Feel free to check out my other videos here: https://www.youtube.com/channel/UC5cIi8_VbiZP17YrnlYJE6w For this combo, you want to moving to the right after each hit/skill you do. Otherwise, the combo's will not hit due to lack of spacing between you and the enemies body. (Basically notice how i'm always moving to the right) X-X into Ankle Cut Rising Cut (Don't hold) *NOTE* Rising cut has about 0.5 second delay AFTER the skill is pressed, due to the animation. Fireart: Fireball (while mid-air after Rising Cut) Heavenly Fireblast (0.5 seconds after Fireart: Fireball. If the delay is more than 0.5 or possibly 0.6 seconds, the enemy's body, will be too close to the ground and will not become an OTG (Off The Ground) hit. Double Jump immediately after Heavenly Fire Blast, while moving to the right. **This will be just the second jump, since the first one is used.**(Moving to the right is crucial for this part, because the combo can drop from being too far away from the enemy's body) Flying Squirrel after the second jump(3 hits) Rising Cut (while mid air after Flying Squirrel) (Air) Spiral Wheel Kunai after Rising Cut