text:
Author | exchange4media News Service | Saturday, Nov 14,2015 8:43 AM
CEAT has announced its association with batsman, Rohit Sharma, for a 3-year bat endorsement deal. Sharma is the first ever player to score two double-hundreds in ODI history, among many other accolades he has bagged in his career. The cricketer will now be seen playing with a bat displaying CEAT insignia in all formats of the game.
Currently part of the Indian cricket squad, Sharma has been a consistent performer across multiple formats of the game. In 2014, Sharma scored his career best 264, the first to be achieved by a batsman in an ODI. With 16 sixes, he created cricketing history by recording maximum sixes in an ODI. Under Sharma's captaincy Mumbai Indians (MI) have won the IPL in 2013 and 2015. He has also led MI towards victory in the Champions League T20 in 2013.
Commenting on the partnership, Anant Goenka, MD, CEAT said, "We are proud to announce Rohit Sharma as the latest Indian cricketer to join CEAT's family. Rohit is not only an extremely talented batsman, but also a great leader and an inspiration to young aspiring cricketers. We believe that his dynamic personality combined with his performance and dependability on the field represents the values that CEAT believes in"
On beginning his new association, Sharma said, "This is definitely an exciting association for me. Partnering with CEAT, who've played a significant role in boosting cricket in our country is indeed an honour. I look forward to playing a long innings with CEAT!"
Programming HeadRadio Mango


text:
I've been chatting with a girl on Okcupid. We had talked about meeting up for coffee today but she couldn't because she has to work but she said she was off Tuesday. So I said that's cool, we can Tuesday then. Well that was 2 days ago and she hasn't sent anything back yet. I don't know if I should message her or not. I didn't try to get her number yet because some people on here said it would be better to set up the date first and then get the number. So what should I do? Any help would be great.
Most Helpful Girl
when someone is interested they find a way not give excuses...message her once and say " are we still on for Tuesday?" and if she doesn't respond then don't sweat it, move along , and she may even message afterward maybe she has a good explanation00|00|0

subset: commoncrawl


text:
who we are
Levels Management is a New York City based boutique brand consulting and artist development firm which provides added services in public relations and event planning. With over 10 years experience in the entertainment & nightlife industry we understand the importance of providing our clients an honest "tailored-fit" approach to representation. Our relationships extend within all facets of entertainment and to all major cities in North America and our clients come from all genres of the industry, from an assortment of brands to recording artists and reality stars.
We provide one on one consultations that help elevate your brand, company or product to an entirely new level.
Brand Management & Public Relations
Bring your brand and your reputation to new heights with innovative marketing strategies.
Event Planning
Let us produce your next launch event, release party, or showcase. Levels Management has the means to bring exposure to your brand to the media and the demographic you need.


text:
A popular heart drug for atrial fibrillation could increase the risk of dementia. The drug is warfarin, which is used to prevent blood clots. The study found that patients taking warfarin had a higher risk for dementia if their blood levels of the drug were often too high or too low.The results remained the same for other patients who didn't have atrial fibrillation but were on warfarin for other heart conditions.
Lead researcher Dr. Jared Bunch suggests the findings uncover two potential concerns: atrial fibrillation patients may be at a higher risk for dementia, irrespective of warfarin use, but warfarin may contribute to dementia if dosages are not optimal. Dr. Bunch said, "If people's levels of warfarin were erratic, their dementia risk was higher, whether they had [atrial fibrillation] or not."
Although the study did not establish a cause-and-effect relationship, Dr. Bunch suggests both atrial fibrillation and warfarin may contribute to dementia as it affects the blood flow to the brain.
To conduct their study, researchers looked at data from 10,000 patients on warfarin either for atrial fibrillation or for other reasons.
Over the course of six to eight years, nearly six percent of atrial fibrillation patients developed dementia, including Alzheimer's disease, compared to two percent of patients on warfarin for other reasons.
Even when the researchers accounted for other factors, including age and comorbid conditions, those in the atrial fibrillation group still had double the risk of dementia, compared to other patients.
Dr. Bunch offered some advice for patients, "If you're doing well on warfarin, there's no reason to worry. But if you're someone whose warfarin doses have to be changed a lot, you could ask your doctor about alternatives."
Also, read Bel Marra Health's article on Type 2 diabetes drug, metformin, safe for the heart, unlike other diabetes drugs.


text:
Dr. Alfred Gardner Garrett, age 87, of Shadowline Drive, Boone, passed away Monday morning, May 21, 2014, at Watauga Medical Center.
Born September 19, 1926 in El Dorado, Arkansas, he was a son of Eschen Boland Garrett, Sr. and Linetta Chambers Garrett.
After retiring as a chemist from DuPont, he taught briefly in the chemistry department at Appalachian State University.
Dr. Garrett is survived by his wife, Marjorie Murphy Garrett of the home; his daughter and son, Christine Garrett and Neilsen Garrett, both of Newark, Delaware; three step-sons, Jett Murphy of Fleetwood, John Murphy of Nashville, and Andrew Murphy of South Carolina; step-daughter, Julia Rummans of Hilton Head, South Carolina; brother, Carl Garrett of Arkansas; and sister, Frances Wynn of Louisiana.
Services for Dr. Alfred Gardner Garrett will be private.
Online condolences may be shared with the family at the website www.austinandbarnesfuneralhome.com
Austin & Barnes Funeral Home and Crematory is serving the Garrett family.
Funeral Homes


text:
Gyptian You are my African queen mp3 1 MP3
Download: http://bit.ly/1gYMZ2x Gyptian You are my African queen mp3 1 Gyptian You are my African queen mp3 1.
Black Face - African Queen [download good quality] MP3
2face Idibia African Queen MP3
2face Idibia - African Queen. You can download the mp3 file here http://www.afrikatoday.com/file_mp3.php?mp3=12.
African Queen with lyrics on screen MP3
african queen with lyrics 10000 views can we get to 20000?? also comment and like? and let me know if i can do another song for you? aslo is in the film "phat ...
Drastic - African Queen (Acoustic) Cover MP3
Here's a lil video I did with the #VivaVideo app on my Android of me Singing an acoustic style cover to Idibia "African Queen" Free Download of the MP3 is ...
African Queen - 2Face Idibia Lyrics MP3
Lyrics to African Queen (with music)
African Queen (Slow Version) MP3
African Queen (Slow Version)
2Face Idibia - African Queen (Sax Remix) + Sheet Music MP3
GET THE TOP RADIO HITS IN SHEET MUSIC FOR ALL INSTRUMENTS: http://hitsheets.com GET BETTER ON THE SAX: ...
Vanessa Mdee & 2Face - African Queen/Nobody But Me - Coke Studio Africa Mash Up MP3
Producer Cobhams mashes up an African classic with an East African chart topper for Vanessa Mdee and 2baba to perform on Coke Studio Africa.
2 face African Queen Key board MP3
2 face African Queen key board.
African Queen (feat. Stereo Luchs) MP3
Beyoncé - Grown Woman MP3
BEYONCÉ Platinum Edition. Available on iTunes: http://beyonce.lk/itunesplatinum Available on Amazon: http://beyonce.lk/platinumam Box Set includes : 2 New ...
Grown Woman
BeyoncéI remember being young and so brave, I knew what I needed I was spending all my nights and days laid back daydreaming Look at me, I'm a big girl now, said I'm gon' do something Told the world I would paint this town, now, bitches, I run this... Ah! 'Cause I put it (down like that, put down like that) And I'm makin' (all these racks, all these racks) And I'm movin' (round like that, round like that) When I do it, (I [...]
C4 Pedro - African Beauty feat DJ Maphorisa (Vídeo Oficial Feat Boity) MP3
C4 Pedro - African Beauty feat DJ Maphorisa Facebook: http://www.facebook.com/c4pedrofficial Instagram: @_c4pedro_ @kingckwa Twitter: ...
2FACE IDIBIA -African Queen (guitar cover) MP3
a cover as requested.. check it out! (SPREAD THE LOVE) www.youtube.com/druRECKOLLECT www.myspace.com/rekollect ...
African Queen remix MP3
Its a remix of the song African Queen... and pictures from fireworks show in Asakusa, Tokyo, Japan, summer of 2009!!! enjoy,, cheers.
African Queen- 2face Idibia MP3
(You Are My) African Queen by 2face Idibia. First time im recording m self singing after about 3 years. I really cant tell if its good but constructive will criticism ...
C4 Pedro - African Beauty feat DJ Maphorisa [Áudio] MP3
C4 Pedro - African Beauty feat DJ Maphorisa Música e letra: C4Pedro Produção: Dj Maphorisa & BLS Vozes: C4Pedro & Dj Maphorisa Mix & Master: Tiago ...
Prince Far I - African Queen Version MP3
Prince Far I - Umkhonto We Sizwe (Spear Of The Nation) Record date : 1984 Mixing Engineer : Sylvan Morris Engineer : Scientist Producer : Roy Cousins ...
Trap Queen Remix Kizomba By Dj Anilson clip ( danse by Chris Py & Booxy) MP3
COVER BY JOSH LEVI DOWNLOAD :https://soundcloud.com/dj-anilson/tra... SNAP : DJ-ANILSON INSTAGRAM : DJ_ANILSON BOOKING : +(33) 623732256 ...
Butterfly by Nameless (Official Video) MP3
East African artist Nameless has always had a very close relationship with Uganda. During one of his many tours , a Ugandan fan challenged him to learn some ...


text:
- Description
What looks like a rather unassuming table with bold legs and glossy lacquer coating, flips open to reveal ample storage underneath. It's this kind of surprise that will keep your guests amused and entertained.Includes
1 End TableDimensions
End Table, 23 1/2"L X 23 1/2"W X 22 1/2"H
More from this collection: Lonia
- Additional Information
Additional Information
SKU 13792788 MPN CM4037RA-E GTIN UPC 847289070092 Brand Furniture of America Collection Lonia Assemble Length N/A Assemble Width N/A Assemble Height N/A Assembly Required Yes Material Wood Finish N/A Styles Contemporary Shapes N/A Shipping Time 1-2 Weeks Free Shipping Yes
- Reviews
Disclaimer: Pictures displayed may include optional products not included in the price. Computer monitors often display color variations. Color swatches are shown if alternative colors are available. Please call us or email us if you have any questions!


text:
The Temple District was the Second City's primary center of religion and academic study, housing many of the shrines and temples of the colonial capital. [1]
Status Edit
It was the first district to be created after the city grew beyond the walls of the Military District on its eastern edge. A secondary wall was built to protect this district. It was the Second City's primary center of religion and academic study, and many scholars and priests chose to maintain private estates within the district. [2] Focused on learning and religious study, the Phoenix and Dragon Clans took an early leadership role in how it was designed and built. Having a residence in the Temple District became a status symbol among the traditionalists. [3]
Appearance Edit
The Temple Districti was marked by a great temple in the center, the Temple of the Seven Fortunes along with several large libraries. The district as a whole was built in an expansive manner to make the area feel open and serene. [4]
Temple to the Lords of Death Edit
The city had a large Unicorn population, so a Temple to the Lords of Death was built. [5]
Inquisitor Temple Edit
In 1198 the Phoenix was granted to build a temple tended by Asako Inquisitors, to protect the citizens from any danger that arose from exploration, [6] the Library of Rebirth. [7] The Phoenix library was the largest repository of recovered lore from the now-lost Ivory Kingdoms. [8]
History Edit
P'an Ku's Madness Edit
In 1199 th Temple District had been almost completely ruined by angry mobs under the influx of the mad dragon P'an Ku. [9]
Dark Naga Assault Edit
In 1199 Dark Naga forces assaulted the city, and the Quelsa led the serpents against the Temple District. [10]
Notable Locations Edit
- Fukurokujin's Library
- Imperial Library
- Imperial Scrivener
- Library of Rebirth
- Nasuo's Lab
- Seppun Temple
- Temple of Tengen
- Temple of the Rising Dragon
- Temple of the Seven Fortunes
- Temple to the Lords of Death
- Training Grounds
External Links Edit
- Temple District (Siege: Heart of Darkness)
- ↑ Second City - The City, p. 7
- ↑ Second City - The City, pp. 7-8
- ↑ Second City - The City, pp. 34-35
- ↑ Second City - The City, p. 35
- ↑ The Shadow's Embrace, Part 3: The Phoenix, by Nancy Sauer
- ↑ Embers of War, Part 2: The Lion, by Shawn Carman
- ↑ Library of Rebirth (Emperor flavor)
- ↑ Scenes from the Empire 28, by Robert Denton, Seth Mason, and Shawn Carman
- ↑ Coils of Madness 4, by Seth Mason & Robert Denton
- ↑ Gen Con 2014: The Heart of Darkness (Storyline Tournament)
|This Imperial Families related article is a stub. That means that it has been started, but is incomplete. You can help by adding to the information here.


text:
- Overview
- Biography
- Songs
- Browse by Format
- Browse by Genres
- Similar Artists
|drum and bass jungle drum n bass dnb electronic
|allmusic discogs lyrics other databases VIAF wikidata wikipedia
Lester Bullocks, 25 June 1953, Kingston, Jamaica, West Indies. In 1971, Dillinger commenced his career as a DJ, working on the sound systems of Prince Jackie and El Brasso, where he initially imitated U-Roy, Dennis Alcapone and Big Youth, before forging his own style. In 1974, he recorded the excellent Freshly for Yabby You, and the following year had a glut of material released, including Brace A Boy for Augustus Pablo, CB 200 for Joseph Joe Joe Hookim and Killer Man Jaro for Coxsone Dodd. It was Dodd who released Dillingers stunning first album, Ready Natty Dreadie, on which he effortlessly delivered 10 strong toasts over a selection of Studio Ones classic rocksteady and reggae rhythms. It is only the first pressing of the album that contains the brilliant title track, as it was subsequently replaced by Natty Kung Fu. Hookim released Dillingers second album, CB 200, which...


text:
Keyston is a male standard chinchilla. He was owned for 10 years and due to family allergies came to the rescue. He is a very curious chinchilla who always wants to see what you are doing. He loves to get his nightly handful of hay and enjoys watching the other chinchillas.
Fostered in Mississauga.
Thank You!
Your message has been sent to Canadian Chinchilla Rescue.
You'll receive a copy, too, at to help you keep track of which pets you've inquired about, and which shelters and rescues you've emailed.
NOTE: Some shelters have physical locations you can visit; some of these shelters may only have pets for a limited time, so please do not wait for a reply—just go visit the shelter! Other organizations are rescue groups run by busy volunteers who may take a while to reply. You can find information about the shelter or rescue group caring for this pet, and their adoption procedures, on the pet's details page on Adopt-a-Pet.com.


text:
Each month, Rated People connects over 50,000 homeowners with quality tradesmen - simply enter your job details in the form above to get started.
We'll match your job to the relevant tradesmen in your area - those interested will pay to see your contact details and call you to quote.
As each tradesman expresses interest in quoting on your job, we'll send you their profile and ratings to help you choose the right one for you.
After the work is complete, return to the site and rate your tradesman based on their quality of work, reliability and value for money.
Gardener / Landscape gardeners
Added 13/06/2017
Services required: lawn mowing and edging, weeding, pruning, hedge trimming Approximate length...
Searching for tradespeople
Tree Surgeon
Added 28/03/2014
take down a large beach tree 40ft tall 2ft trunk telephone cable running through the middle...
Searching for tradespeople
Added 29/01/2012
Conversion of utility room to a second sitting room and conversion of existing space to a second...
Searching for tradespeople
Managing your job is easier with the Rated People Homes App, download it today.


text:
Delivery Assistant - Everett, WA
For over 40 years, AA Party Rentals and ABC Special Event Rentals have led the Seattle market in customer service and quality of product. In 2015, these two businesses merged and rebranded under CORT Party Rental. While the name and look of the company may have changed, the same great people are here to provide you the exceptional service and solutions you can rely on for another 40 years and beyond.
CORT Party Rental provides expertise and equipment for all types of events. We specialize in dcor and equipment for events such as weddings, festivals, concerts, corporate conferences and more.
Loads inventory into truck, assists in safe operation of vehicle while in transit, and unloads inventory.
Job Requirements
Pulls inventory from warehouse racks and stages it for loading.
Loads product onto the truck, and checks product against contract.
Gives directions to helpers (non-skilled/temp labor) in moving, counting, and loading product.
Protects product from damage using best demonstrated practices.
Loads truck, distributing weight evenly, forming compact load, and utilizing space.
Conducts pre-use inspections and services truck.
Safely delivers and installs... View Full Description
Apply Now
Critical Need Jobs
jobs by
Browse other Trucking Jobs in Washington
Browse all Trucking Jobs in Washington


text:
November, 2016: My short story, "I am not Edward Snowden," has just been published in Blunderbuss magazine, online and in print.
November, 2016: The Principles and Practice of Narrative Medicine has just been published by Oxford University Press! I have two chapters in the book, both about creativity and its use in clinical practice.
- December 7, 2016: Narrative Medicine Rounds: Book Launch for The Principles and Practice of Narrative Medicine, just published by Oxford University Press! Faculty Club, Columbia University College of Physicians and Surgeons


text:
Under a new program, Galt residents can finance energy efficiency upgrades and repay the cost over time through their property tax bills.
On Tuesday, the City Council narrowly approved a partnership with Sacramento County to allow property owners to voluntarily finance certain upgrades such as the installation of solar panels or dual-pane windows under the county's Property Assessed Clean Energy (PACE) program. This not only reduces the upfront cost, but allows the improvement fees to be shared with future property owners, according to a presentation made at Tuesday's meeting.


text:
Trucks For Every NeedFAQ
PORTER, is a mobile based aggregator for commercial vehicles, providing transparent and convenient platform to customers and partners to keep goods moving. With a fleet of 3000 vehicles, Porter currently offers its services in Bengaluru, Chennai, Delhi NCR, Hyderabad, Mumbai.
High Earning
On Time
Discount on AMC
& Insurance
More Trips
" ...After joining Porter, I started getting a lot of customers. As a result, my financial condition improved significantly and now I earn at least ₹35,000 - ₹40,000/- per month. Porter has made my life awesome !!"
" ...A friend referred me to join Porter. The biggest benefit is constant availability of trips. I started off with a single vehicle and now I am a proud owner of 3 vehicles and earn ₹30,000/- per vehicle. Joining Porter was the best decision of my life"
" ...I have been into goods delivery for past 14 years and was earning around ₹16,000/-. After attaching with Porter since 2015, my income has grown to ₹40,000/- per month. I receive all my payments on time. Porter made my life simple."
" ...Getting business at vehicle stand has become very tough today due to intense competition. But with Porter's mobile app, transparent fare structure and standard pricing, my life has become easier. Now I don't worry about finding customers and getting payments."
"...Despite being new to Chennai, Porter's app, with inbuilt map and navigational capability, never made me feel new to city. Payment is done on time and their vendor helpdesk provides immediate solutions to all my issues."
"...Earlier there were many restrictions on earnings because I got limited trips in market which were only from my known customers and to nearby places. But, with Porter there are no such restrictions, there are a lot of orders throughout city. I enjoy being a part of Porter family."
How to Join PORTER ?
You can fill the form on PORTER partner page and our vendor team will get back to help you with the on-boarding process at PORTER.
What documents and permits do I need to on-board PORTER?
Vehicle related documents :
RC book | Fitness certificate | Insurance | Pollution Control
Owner/Driver documents :
Driving License | PAN card | Bank Details | Address Proof
Is there any registration fee to join PORTER?
PORTER charges a nominal registration fee* of Rs 500/- at the time of on-boarding.
Which type of vehicle one can attach to PORTER?
Porter is involved in intracity movement of goods and on-boards all vehicle types with carrying capacity of 1 ton, 2 ton and 3 ton for our Spot engagement.
How can I earn?
Being an aggregator, Porter has a huge customer clientele which uses PORTER platform to get vehicles for its daily logistics requirements. With 3000 vendors on-board and approx. 40,000 unique customers, PORTER provides its vendors with regular trips.
How much I can earn per week?
Based on your activeness on our platform, you can earn upto Rs. 12000-15000/- per week*.
How I can check my earning & payouts?
PORTER has an advanced driver app which provides all details pertaining to daily earning and weekly payouts.
Can I refer my friends and colleagues to join PORTER?
Yes! We encourage our vendors to promote PORTER among their peers and refer them to join us. In return they deserve a goodwill bonus on each successful on-boarding.
I have a query. How I can get in touch with PORTER?


text:
The vehicle in your driveway is head and shoulders above the rest when it comes to all the cars, trucks, and SUVs on the road today. Car aficionados appreciate the good looks built into each Oldsmobile, and understand that only the highest quality replacement F85 parts will suffice when your car or truck needs fixing. Anyone who operates a Oldsmobile knows that when it comes to fixing their car, truck, or SUV only the most superior replacement parts will do. While it may not be difficult to buy replacement parts for Oldsmobile vehicles, these days the necessity for nothing but the highest quality are required to repair your car or truck.
You never realize what you've got until it's gone, and the same is true for your power steering. If you were to analogize your Oldsmobile F85 steering system with the human body, the power steering pump would be its heart and the fluid would be its blood. Your Oldsmobile F85 power steering pump is powered by the crankshaft, and connected via a drive belt between the crank and the pump pulley. It keeps the power steering fluid under constant pressure so that your rack and pinion can make steering easier. You will notice a gradual loss of your power steering if the power steering pump is leaking, but if it seizes you can expect no power steering at all.
Don't let a Power Steering Pump repair bring about the demise of your car, truck, or SUV, and don't gamble with its condition by using anything but the best replacement parts. Given all the trouble that can be involved with installing a part to fix your car or truck, let www.carpartsdiscount.com be the first place you go to make buying it easy. Get your F85 back on the road with the best repair parts from the experts at Car Parts Discount. When it comes to Oldsmobile F85 Power Steering Pump parts, you can find them for 1972, 1971, 1970, 1969, 1968, 1967, 1966, 1965, 1964, 1963, 1962, 1961 models by clicking the appropriate year below.


text:
Product code: 26736
Despatched in 3-5 days.
Meridian Coconut & Peanut Butter blends natural peanut butters with pure coconut and a dash of honey before it's grinned until nearly smooth to offer an indulgent yet healthy combination of taste and texture.Peanut Butter benefits:- a rich source of vital essential minerals which are beneficial for heart health and bones, hormone production, digestion, thyroid function and reducing anxiety- contain beneficial amino acids which your body makes into the feel good brain chemical, and helps to reduce blood pressure.- contains roasted peanuts with their natural skins still on that not only locks in valuable fibre,but the skins are also rich in resveratrol, an anti-oxidant that is increasingly linked to longevity and this contributes to healthy living.Coconut Butter benefits:- Can improve blood cholesterol levels - may lower your risk of heart disease- is loaded with saturated fats, which actually do not harm the blood lipid profile like previously though- highly beneficial for healthy skin and hair- a rich source of short and medium-chain fatty acids (MCFAs), in particular lauric acid and also rich source of nutrients like vitamin E, Vitamin K and Iron. Celery free, Gluten free, Eggs free, Fish free, Lupin free, Milk (including lactose) free, Molluscs free, Mustard free, Soybeans free, Sulphur dioxide or sulphites free.Suitable for vegetarians, Suitable for coeliacs, Suitable for diabetics, Free from GMOs (genetically modified organisms), Free from transfats and hydrogenated oils, Free from added artificial colourings, preservatives and flavourings, Free from Dairy, Free from Yeast.
Roasted Peanuts with whole skin on (69%), Coconut (28%), Honey
Contains peanut.Not suitable for other nuts or sesame allergy sufferers.
Delicious spread on toast or add to smoothies, cakes and bakes, or simply with just a spoon, as part of a varied and balanced diet and healthy lifestyle.
Sign up for the latest news, offers and ideas.
eChemist.co.uk © 2017. All rights reserved.


text:
Marty Bags - You're Not me (Yu-Gi-Oh the movie original soundtrack) MP3
I KNOW anyone who ever watched yu gi oh when they where younger knows this song. I was reminded of it today when i had an unusual conversation with my ...
Yu Gi Oh! 10th Anniversary Movie Super Fusion! Bonds Beyond Time Ending HQ MP3
Yu Gi Oh! 10th Anniversary Movie Super Fusion! Bonds Beyond Time Ending ENJOY :)
Yu-Gi-Oh The Movie: One Card Short MP3
Life's just a game we all wanna win Keep your hand a secret, unleash the monsters within Sometimes it's over before it's even begun If someone else gets hurt ...
Yu-Gi-OH! Soundtrack - You're not me [w/Lyrics] MP3
I was bored...xD...and making videos with the lyrics of the songs you like is fun :D enjoy!
We're Yugioh song MP3
click on this to reset the video quick 0:00.
Yu-Gi-Oh! the Movie - Soundtrack - 01 - You're Not Me MP3
[Yu-Gi-Oh] Bonds Beyond Time Movie Rap MP3
Jaden, Yugi, and Yusei Rapping Where to watch it: https://www.youtube.com/watch?v=4UScz7Ne3cI Subscribe to my friends: Zack: ...
Jean - Step Up - Yugioh The Movie Soundtrack MP3
All credit goe sto the artists and the creators of yugioh the movie. Lyrics: Step Up, Step up, try to get through me, but at the end your gunna see, the only way it's ...
yugioh your not me MP3
please rate and comment this song is your not me from Yugioh the movie the pyramid of light.
Step Up MP3
THIS IS NOT COPYRIGHTED TO ME, THE RIGHTS GO OUT THE PROPER RIGHTS HOLDERS!!! ** REPEAT! I DO NOT OWN THIS!!!*** Yu-Gi-Oh!: The Movie ...
Yu-Gi-Oh! The Movie - The Unreleased Soundtrack: Blue Eyes Shining Nova MP3
Yu-Gi-Oh! the Movie - Soundtrack - 05 - Shadow Games MP3
Dark Side Of Dimensions Movie Trailer 4 (Yu-Gi-Oh! Arc V Ending 5) MP3
New scenes for the movie in Arc V's latest ending song.
Yu-Gi-Oh! The Movie - Unreleased Soundtrack: Great Sphinx Theinen MP3
Great Sphinx Theinen ----------------------------------- From: Yu-Gi-Oh! The Movie - Pyramid of Light Composer: Gil Talmi Please Like and Subscribe! More Yugioh ...
Shadow Games MP3
Here are the lyrics to 'Shadow Games' from the Yu-Gi-Oh movie soundtrack. I do not own Yu-gi-oh or the music. Copyright belongs to Wayne Sharpe.


text:
We are looking for quality candidates – both recent graduates and junior/senior consultants – in all activity sectors.
Within a context of individual professional growth, our professional ladder provides for a differentiated seniority hierarchy based on these basic parameters: accumulated experience both within and/or external to the group; proven expertise, competency and professional autonomy; business development skills.
In order to create the conditions under which the competencies of each The European House – Ambrosetti consultant can be developed properly and quickly and in line with his or her own aptitudes, we have developed a competency development program. The program involves a number of different activities:
The qualifications requested, professional opportunities and selection methods for candidates who have graduated within the last 12 months.learn more
What we offer candidates with consulting and business experience. The professional pathway inside the firm and selection methods.learn more


text:
So i have set my signature permission to allow, i use the analyze permission tool, and it says i am allowed to edit my signature.
But i don't see the option for it.
I have tried by just going to this url:
http://www.website.com/index.php?account/signature and it says the following:
You do not have permission to view this page or perform this action.
Anyone has any idea why is that?


text: 35 photos $355,000 80 acres 390th Ave., Wagner, SD 57380 - Charles Mix County 80 acres Wagner, SD This smaller parcel that is a great addition to an existing operation or the perfect starter tract whether you are wanting to get into farming or a wing shooting paradise. The property is ready for its full potential to be used as a waterfowl honey hole. Red Lake GPA adjoins the property to the north providing hundreds of acres of wetlands for migrating and nesting waterfowl. On my arrival, dozens of nesting birds were taking flight as I intruded into their prime habitat. The installation of a blind or 2 will have you shooting ducks and geese on this income producing farm. The deer hunting should not be overlooked. Red Lake and its thick marshy grasslands are more than ideal to hold mature whitetails and this property provides a nice set up Jason Schendt Whitetail Properties


text:
Are you a Barry M fan? Or a blue nail fan? Looking at my nail varnish collection, apparently I'm both.
Friday, 8 August 2014
Are you a Barry M fan? Or a blue nail fan? Looking at my nail varnish collection, apparently I'm both.
14 comments:
I really like all the colors!ReplyDelete
xx, Be || lovefrombe
Such lovely blues! I love blue grape.ReplyDelete
Chloe The Redhead | Beauty, Fashion & Lifestyle
ooooh love the blue gradient!ReplyDelete
Emma from http://www.masqueradebelle.co.uk
I love your blog! This is a great post :)ReplyDelete
What makes you beautiful
I think Blue Grape has to be my favourite - Perfect for festival season! :)ReplyDelete
Fleur x
You have a lovely blog, lovely post! Such nice photos, would never think to try blue polish, but it looks fab!!ReplyDelete
I prefer a blue polish to a pink - bit less girly! Anyone clue me up on how the Gelly texture dries? Is it designed to look very shiny?ReplyDelete
Love the motion video! The colours are awesome!ReplyDelete
Josie XOXO
Fashion Mumblr
lovely colours! xReplyDelete
Amazing shades! Great post :)ReplyDelete
Maybe follow each other on bloglovin? Let me know :)
Lovely colours :) I love your blog and I have followed you with Bloglovin . If you ever get a chance to check out my blog I would be delighted. Thanks!ReplyDelete
Camille xo
I love Barry M polishes! You have a lovely selection of blues and greens!ReplyDelete
The Velvet Black // UK Style, Beauty and Lifestyle Blog
I really love Barry M's gelly range, the shade range is brilliant! xReplyDelete
Beauty Soup || UK Beauty Blog


text:
Arctic gusts roared past the gleaming steel buttresses of Metamind Tower 14, Floor 42, belying the quiet focus of its inhabitants. Within, the chief warmind of the Steel Collective analyzed the reports streaming in from his Doppelbot spy network.
The Demara-worshiping netherscales have lost their goddess's blessing, causing them to fall to infighting among the naga clutches. The poisonous yeti malice mongers have abandoned them and are now making lighting raids in the eastern outposts, with Arrachtor-summoned elementals and even the once gentle leafkin spotted among their bands of corrupted forest spirits and insects.
Shard powered armor remains an unrealized advantage as the Grove Queen rebuffs all diplomatic and "tactical" attempts to acquire enriched deposits in the upper Deepbranch marshes. The Infinity Warden and his sycophantic sage escorts have been spotted consorting with the queen and her plant and tuskin servants, Saint Nuada included.
The splinter cult, Forgeheart, continues to accumulate disenfranchised faithful from the Great City's quarters. Ironbeard embraces a messianic role, as the "Breaker of the False Anvil". His prophets spread the news of the "forgespark within"; even the Aerial Squadron commander, Uriel Ironwing, has abandoned its post to join the cause. Clearly, the unmanned battlements and dwindling stock of armaments no longer matter to the former "Hammer of Anvillon". Rumors also intimate that Ironbeard preaches of Nekrium monstrosities that would prey upon the faithless– Cyrus the Merciless Grimgaunt, Iniog the Carrion Demon, the Lichmane Drakes– as a means of keeping his flock in line.
Ghox pursed his gray lips; his quartet of metamind servitors continued to operate their workstations as their leader silently considered the situation at hand. Tactics, logistics, and acceptable losses flashed through the Paragon's mind.
*A gray mist silently billowed from a crackling nether portal*
In crisp, calm tones he ordered, "Send the Twenty-Third Frontline Squadron under Infiltrator Crux to assassinate the Umbruk raid leaders. Keep alert for any of the Arrachtoron "lightning titans" and pray we are past the leafkin's spawning season; even our justicars cannot contain them."
*Ash-gray claws birthed from the violet world-tear as the portal swiftly grew on size*
"Send Commander Drix to negotiate a resumption of work duties with the "Forgeheart" cultists Hopefully they are not truly consorting with Nekrium monstrosities. As for Nova.."
Azure lightning surged through the war chamber, enervating Ghox and his minions. The metaminds' weakened limbs hurled them to the floor as a Tarsian demon skittered from an adjacent hall, jet-black claws scratching grooves in the steel flooring as it approached the Paragon.
Ghox fought his shriveled muscles, stretching for a beam pistol holstered at his waist, as the chuckling demon pierced his flesh with its talons and lifted him into the air. The demon eyed the cerulean fluid leaking from its meat-puppet's wounds with a nightmare smile and whispered broken-glass words into its target's ears, "The Great Xerxes wishes to see you you, but first we have some fun." With its free claw, the Tarsian "patron of torment" casually snapped Ghox's upper-right wrist, grimacing delightedly at the metamind's spasms of pain. "That was but a taste, worm. You don't need to see what's coming next…"
Icy, black fluid splashed in Ghox's eyes, gumming his already-clouded eyes. He felt himself sinking downward into darkness, but was shocked to find himself still alive in the seconds that followed. His vision cleared to focus on a luminous blue fist, jutting from a gaping hole in the demon's chest. It retracted from the wound, reaching out to grasp him and raise him to his feet.
Its owner, one of the console operators, was sheathed in a flickering forcefield; similar to the telepathic film exuded by all metaminds, but significantly brighter and, apparently, more impactful. At his feet, lay the outstretched, heaving forms of the other operators; they grasping his ankles, visages of concentration etched on their faces.
Ghox, heaving for breath and cradling his shattered third wrist, pondered this development. Telepathic force weapons. Strength in Unity. A missing piece to the puzzle?
He coughed bloody sputum and whispered, "Operator, you have granted us great insight. It is time we made you a commando."
(Chronicled by MagaFortOss)
Greetings, Forgeborn! I'm Wendell. You may remember me from such articles as "Killin' Killion" and "Matrix Warden: An Analysis Using Heirarchical Inversion Theory". If you do, hello from an alternate universe!
Metaminds featuring Ghox, Metamind Paragon and Drix, The Mindwelder is one of the classic decks from the early days. It fell by the wayside as more powerful decks came to prominence, but the archetype has received a boost recently with the arrival of G.S.F. Commando. Here's my take on a modern metaminds deck.


text:
|Story Views
|Last Hour:
|Last 24 Hours:
Small Caliber Ammunition Market analysis is provided for global market including development trends by regions, competitive analysis and major development forecast of the small caliber ammunition market. Small caliber ammunition is .50 caliber and below. Conventional small caliber ammunition in production and deployment consists of 5.56 mm, 7.62 mm, 9 mm, 10- and 12-gauge, .22 caliber, .30 caliber, .38 caliber, .45 caliber, .300 WinMag and .50 caliber.
Browse Detailed TOC, Tables, Figures, Charts and Companies Mentioned in Small Caliber Ammunition Market Research Report @ http://www.360marketupdates.com/global-small-caliber-ammunition-market-by-manufacturers-regions-type-and-application-forecast-to-2021-10223034
Market Segment by Manufacturers, this report covers
Scope of the Report:
This report focuses on the Small Caliber Ammunition in Global market, especially in North America, Small Caliber Ammunition Market in Europe and Asia-Pacific, Small Caliber Ammunition Market in Latin America, Small Caliber Ammunition Market in Middle and Africa. This report categorizes the market based on manufacturers, regions, type and application.
Market Segment by Regions, regional analysis covers
Get Sample PDF of Small Caliber Ammunition Market Report @ http://www.360marketupdates.com/enquiry/request-sample/10223034
Market Segment by Type, covers
Market Segment by Applications, can be divided into
Key questions answered in the report:
Have Any Query? Ask Our Expert for Small Caliber Ammunition Market Report @ http://www.360marketupdates.com/enquiry/pre-order-enquiry/10223034
No. of Report pages: 112
Price of Report: $ 3480 (Single User Licence)
About 360 Market Updates:
360 Market Updates is the credible source for gaining the market research reports that will exponentially accelerate your business. We are among the leading report resellers in the business world committed towards optimizing your business. The reports we provide are based on a research that covers a magnitude of factors such as technological evolution, economic shifts and a detailed study of market segments.
Mr. Ameya Pingaley
360 Market Updates
+1 408 520 9750
Email – email@example.com
JasonA: Gripping! You Need to See This! Mighty, Powerful Signs Across the Globe! This Is Not Science Fiction! (Videos)
Feds Are Flipping Out Over This Little "Crypto" Secret Leaked by an Insider!
Couldn't Watch the Boston Protest Live? Here's Full Coverage and Police Clashes (Videos)
Live: Boston
Wake-Up, People! Alt-Left, Alt-Right Run by the Same Forces…The Goal is "Divide and Conquer"
Stanley Kubrick, Steven Spielberg and Hollywood Are Weaponized Tools of Mass Manipulation (Video)
American "Left"- America's New Taliban?
'In This 2nd American Civil War, Whose Side Are You On?': Pat Buchanan
Liberty and The Seed to The Civil War
The Invasion of Canada
God Sends a Strong Delusion
Why There Will Be No 11th Hour Debt Ceiling Deal
JasonA: Gripping! You Need to See This! Mighty, Powerful Signs Across the Globe! This Is Not Science Fiction! (Videos)
Electromagnetic Shutdown - All Cellphone Towers, Satellite Dish, Digital and Sonic Equipment
Solar Eclipse Alert for Monday, August 21, 2017!
Nigel Farage Reacts to BarceIona - Tucker Carlson Tonight Video
The Kurds Are About to Blow Up Iraq
How to recharge mobile in free forever
Unethical: PETA Fined $50,000 After First Stealing Then Executing a 9-Year-Old Girl's Pet Chihuahua 'Maya'
Hate Groups Clash This Time In Boston As 20 Black Lives Matter Thugs Arrested For Pounding On Police Vehicles
PayPal Suspends Jihad Watch After ProPublica Hit Piece Against Anti-Jihad News Sites Boycott Paypal
A Stunning Solo Performance by Julian Lage: "Study for Electric Guitar"
Arrest Records Prove Trump Was Right!
Putin Tells America: America - Keep Your Guns!
A Warning from Orwell Is Echoed by Trump in War Over Monuments
Chicago Traffic Report Captures Red Orb Object With Tail (Video)
Parents Concerned Over Recording of Teacher's Political Rant (Video)
Fox News Camera Catches a UFO Buzzing the US Capitol! Plus Giant Mummies on Display in Peru - Mystery of Ancient Kingdom (Videos)
It's a dangerous time!
The New Civil War


text:
Location: The Lake
Role: Vagrant
Status: Day
Items: N/A
" Show me the power that Ozman taught you." Moir stood there, waiting to see if the clone would throw the first punch. She had a feeling he was waiting for her to throw the first punch but she hesitated.
"I don't have to show you anything." Moir ran up to the clone, so close that her face was only inches away. She brought her left fist into the clone's face with all the strength she said in that arm. Moir could hear the grating of his teeth on each other and she could feel the blood slowly form in his mouth. She was definitely not impressed with the way Rod was acting. Men and there all so mighty power....Moir shook her head in disgust and spit at the clone's feet.
"Meril would be ashamed to see you this way."


text:
Through tax day Jewel is offering an extra $30 if you purchase a $300 gift card. (Also $20 if you purchase a $250 GC) I don't know if they'll let you do it more than once, and I do realize not everyone can afford it, but they're giving away free Jewel money! Thanks, Kate!
Free money from Jewel for spending tax money there
March 18, 2009 by 1 Comment
You can do up to $1250 pre-bonus, so 5 $250s or 4 $300s.


text: Paddy harvesting at - Nenmara village Lights On Light Off Views: 1372 Comments Nenmara is a small village in Palakkad, which is famous for its festival Vallengi Vela and agrarian lifestyle. This is also one of the villages of Kerala where the traditional paddy cultivation is carried out. This video shows you a scene from Nenmara, an agrarian village where a harvest of paddy is going on. Tags: Nenmara, Palakkad, Small village, Kerala, Festival Vallengi, Agrarian lifestyle, Paddy cultivation, Farming, India, India tourism


text:
Yeast 5 – an expanded reconstruction of the
Efforts to improve the computational reconstruction of the
Yeast 5 refines the biochemical reactions included in the reconstruction, particularly reactions involved in sphingolipid metabolism; updates gene-reaction annotations; and emphasizes the distinction between reconstruction and stoichiometrically constrained model. Although it was not a primary goal, this update also improves the accuracy of model prediction of viability and auxotrophy phenotypes and increases the number of epistatic interactions. This update maintains an emphasis on standards compliance, unambiguous metabolite naming, and computer-readable annotations available through a structured document format. Additionally, we have developed MATLAB scripts to evaluate the model's predictive accuracy and to demonstrate basic model applications such as simulating aerobic and anaerobic growth. These scripts, which provide an independent tool for evaluating the performance of various stoichiometrically constrained yeast metabolic models using flux balance analysis, are included as Additional files
Additional file 1
Function testYeastModel.m.m.
Click here for file
Additional file 2
Function modelToReconstruction.m.
Click here for file
Additional file 3
Function fluxDistribution.m.
Click here for file
Yeast 5 expands and refines the computational reconstruction of yeast metabolism and improves the predictive accuracy of a stoichiometrically constrained yeast metabolic model. It differs from previous reconstructions and models by emphasizing the distinction between the yeast metabolic reconstruction and the stoichiometrically constrained model, and makes both available as Additional file
Additional file 4
Yeast metabolic network reconstruction.
Click here for file
Additional file 5
Yeast metabolic network model.
Click here for file
Efforts to improve the computational reconstruction of the
Though a GEM may be considered finished when it is sufficient for a particular modeling application, the effort to build a complete and accurate GENRE is ongoing as biochemical research continues (even information that is fundamental to the construction of a GENRE, such as genome annotation, is considered to be a working hypothesis and subject to ongoing revision
The Yeast Consensus Reconstruction has benefited from the continued involvement of the broader research community. Previous updates to the Yeast Consensus Reconstruction
Therefore, we decided to undertake an update to the Yeast Consensus Reconstruction to refine the biochemical reactions included in the GENRE, particularly reactions involved in sphingolipid metabolism
We have incorporated the results of these efforts to the consensus reconstruction to produce Yeast 5. Yeast 5 expands and refines the yeast GENRE and improves the predictive accuracy of the yeast GEM. Further, it differs from previous reconstructions and models by emphasizing the distinction between the yeast GENRE and GEM, and makes both available as separate systems biology markup language (SBML) files
Improvements to facilitate community use and collaboration
As a consensus reconstruction and model, Yeast 5 will depend upon community use and suggested modifications for future improvement. The Yeast 5 GENRE may serve as a resource for construction of new models, of both genome and smaller scale. Thus, in addition to the emphasis on standards compliance, the Yeast 5 SBML files include coding conventions to facilitate use by popular software. Recognizing that MATLAB is a commonly used platform for systems and computational biology
Yeast GENRE changes
The Yeast 5 GENRE is an evidence-driven biochemical knowledge-base. It does not include the low-confidence or hypothetical reactions and metabolites required to conduct FBA, nor does it include constraints on reaction reversibility which may be added in the course of model-building. Since it does not include compounds such as "biomass", reactions such as "growth", or hypothetical intercompartmental transport reactions, the Yeast 5 GENRE is more specific than Yeast 4, which did not differentiate between GENRE and GEM. It contains 1418 metabolites which participate in 2110 reactions, catalyzed by 918 verified
Additional file 6
Table S1. ORFs in Yeast 4 not in Yeast 5.
Click here for file
Additional file 7
Table S2. ORFs in Yeast 5 not in Yeast 4.
Click here for file
In addition to being more specific, the Yeast 5 GENRE is also more complete than Yeast 4. Sphingolipid metabolism has been acknowledged to be a poorly reconstructed portion of the yeast metabolic network since the first yeast GEM, iFF708
Yeast GEM changes
The Yeast 5 GEM includes biomass demand functions and low-confidence reactions such as intercompartmental transport reactions which enhance network connectivity and enable FBA. It also includes reaction directionality constraints to improve the accuracy of model phenotype predictions and exchange reactions which allow model users to simulate a growth medium. The Yeast 5 GEM includes more reactions and metabolites than previously published yeast GEMs (Table
Additional file 8
Table S3.Genes considered essential.
Click here for file
Since the Yeast GEM includes both general classes of compounds (e.g."fatty acid") and specific members of these classes (e.g. "octanoate"), we use non-reversible encapsulating reactions called "isa" reactions to provide pathways from specific to generic compounds (e.g., octanoate "isa" fatty acid). The use of "isa" reactions is discussed further in the Discussion section. To accommodate the more specific biochemistry included in the Yeast 5 GENRE, the Yeast 5 GEM includes 261 "isa" reactions, compared to 162 in Yeast 4. Additionally, the Yeast 5 GEM includes 2 different lipid pseudoreactions, which create the "lipid" portion of biomass (details of the Yeast 5 GEM biomass definition are included as Additional file
Additional file 11
Table S6. Biomass Definition.
Click here for file
Yeast GEM performance
Though improving gene essentiality predictions was not a primary objective of updating Yeast 4, simulations using Yeast 5 have increased agreement with a list of essential genes and genes which cause auxotrophies; more realistic prediction of internal fluxes; and increased number of genetic interactions. Additionally, simulations with the Yeast 5 GEM predict auxotrophies resulting from gene deletions better than other recent yeast GEMs, and predict gene essentiality with accuracy comparable to other recent yeast GEMs (Table
Gene essentiality predictions
Since the phenotype resulting from a gene mutation is dependent upon media and environmental conditions as well as changes to the metabolic network, the use of gene essentiality predictions as a metric for model evaluation requires careful definition of both simulation assumptions and of the data set used for comparison between simulation and observation. This is particularly important if such a metric is to be used for comparison of different GEMs. We document our approach to simulating gene essentiality in Materials and Methods, and in the testYeastmodel.m MATLAB script included as supplemental material.
The Yeast 5 GEM includes reactions annotated with 918 genes. 144 of these genes are included in a list of essential genes we compiled from the Saccharomyces Genome Deletion project
The results of single gene deletion simulations conducted via FBA of the Yeast 5 GEM using a simulated glucose-limited defined media are summarized in Table
The model predicted that biomass could not be produced following deletion of 101 of the of the 214 genes included on the essential or auxotrophy-inducing gene lists (true negative results), but that biomass could still be produced following deletion of 113 genes included on those lists (false positive results). Thus, the model has a 47% specificity for this list of essential genes. If the model simulation predicted that biomass could not be produced following a gene deletion, the deleted gene was listed as essential or auxotroph-inducing in 83.5% of the cases (an 83.5% negative predictive value).
Comparing Yeast 5 GEM knockout simulations with our list of essential and auxotroph-inducing genes yields a geometric mean overall predictive accuracy (as suggested by
To extend our analysis of Yeast 5 GEM simulation capabilities, we conducted additional FBA growth simulation focusing on the 70 genes included in both the Yeast 5 GEM and the list of genes whose mutation or deletion causes auxotrophies. In 51 single-gene deletion simulations, the model predicted that biomass could not be produced in minimal media but could be produced in a supplemented media, the expected behavior for an auxotroph mutant (see Materials and Methods for more information about our approach to simulated media). In 17 cases, model simulation predicted that biomass could be produced in minimal media, and thus did not accurately predict the auxotrophic phenotype. In 2 cases, model simulation predicted that biomass could not be produced in either minimal or maximal media, and so the model incorrectly predicted that the gene deletion could not be saved by media supplementation.
Auxotroph phenotypes have not previously been a metric used to evaluate yeast GEMs. We found that simulation with the Yeast 5 GEM had better agreement with observed auxotrophic phenotypes than simulations conducted with other previously published GEMs (Table
Optimal solutions found when conducting FBA of the Yeast 5 GEM to maximize biomass flux include fluxes through key internal reactions, a flux distribution which better matches fluxes observed
Recognizing recent efforts to investigate system-level organization of cellular metabolism via the phenotypic effects of multiple gene deletions using yeast GEMs
Additional file 9
Table S4. Auxotroph-inducing genes.
Click here for file
Research to expand our understanding of yeast metabolism and biochemistry is ongoing, and the process of integrating established biochemical knowledge into computational reconstructions lags research advancements. Thus, the Yeast 5 GENRE is not a complete reconstruction of the yeast biochemical network, and though it offers improvements over earlier models, simulations using the Yeast 5 GEM do not fully reflect observed biological phenomena. GENRE and GEM limitations suggest opportunities for future efforts to improve computational reconstruction of established biochemistry and can highlight portions of metabolism that are ripe for further research
A limitation of the Yeast 5 GENRE which suggests future opportunities for improving the Yeast Reconstruction is that due to the lack of information about enzyme specificity or the metabolic significance of variation among similar chemical species, the Yeast 5 GENRE uses general classes of chemical compounds rather than the enumeration of many similar compounds. For example, Yeast 5 generalizes the many possible triglyceride compounds which may be synthesized from fatty acyl moieties of varying length
As with other reconstructed metabolic networks, the continued existence of blocked pathways (Table
Optimal solutions found when conducting FBA on the Yeast 5 GEM may include fluxes that differ from those observed in yeast: we have found optimal solutions in which mitochondrial coenzyme A is synthesized
Due to varying interpretations of experimental evidence, uncertainty of metabolic mechanism, and varying approaches taken as modelers work to reconstruct different portions of metabolism, it is likely that the Yeast 5 consensus reconstruction has additional limitations which will be discovered as it is used. These limitations provide opportunities for continued research to improve the computational reconstruction and simulation of the yeast biochemical network. Thus, though Yeast 5 consists of a more complete reconstruction and more accurate model of yeast metabolism than previous efforts, the goal of building a complete and accurate computational reconstruction of yeast metabolism must remain an ongoing community effort.
Yeast 5 is the most recent update to the consensus reconstruction of the yeast metabolic network. It consists of a genome-scale reconstruction (GENRE), a genome scale model (GEM), and MATLAB scripts designed to facilitate evaluation of yeast GEMs and to demonstrate simulation and analysis using the COBRA and SBML toolboxes. This update improves the consensus reconstruction's coverage of established biochemical knowledge, and improves the predictive ability of simulations using the yeast GEM. The included scripts lower the barriers for the research community to use the model and to contribute to the collaborative effort to improve the computational reconstruction of yeast metabolism.
Models and reconstructions
We emphasize the distinction between a reconstruction, or GENRE, and a model, or GEM, to more clearly delineate the established biochemical knowledge of a reconstruction from the assumptions and hypotheses that are required for modeling and simulation. This distinction makes the modeling process more transparent and reproducible, essential attributes for community-based scientific efforts such as the consensus reconstruction of yeast metabolism.
Evaluating yeast reconstructions and models
As evidence of improvements to the consensus yeast reconstruction, we have presented a comparison of viability, auxotrophy predictions, and genetic interaction effects, produced using FBA of the Yeast 5 GEM and other metabolic models (Table
Expanding the reconstruction of a metabolic network would be expected to increase both dead-end pathways and network redundancy. Dead ends would be introduced through the inclusion of established knowledge regarding pathways that are not fully elucidated. In such pathways, the production of intermediates may be established, but their fate is not yet known. In such cases, expanding the reconstruction of established knowledge would not be expected to improve a model's ability to predict single-gene mutant viability, and so such metrics would not reflect the expanded scope of the reconstruction. Another example of network expansion which may not be reflected in single-gene deletion metrics is expanding the reconstruction's coverage of isoenzymes. Network redundancy increases through expanded inclusion of isoenzymes, which introduce parallel metabolic paths for the production of chemical intermediates or products. In the absence of regulatory constraints (which are beyond the scope of a metabolic reconstruction), these parallel pathways would increase the rate of false positive prediction since a metabolic pathway from substrate to product would exist in the reconstruction, even if a given isoenzyme were individually insufficient to support growth
A second problem with metrics based upon lists of essential genes is that gene essentiality is dependent upon strain, media, and environmental conditions (for example,
That essentiality metrics must be used with care and considered in context is not to say that such metrics are without value, however. Model simulation results that differ from
Like auxotroph phenotype predictions, predicted epistatic interactions have not previously been used as a metric for comparing yeast GEMs. And like other metrics based upon phenotypic prediction, the use of epistatic interactions must also be qualified. Specifically, expansion in the number of reactions in the model is a likely contributor to the amount of simulated epistasis. The number of genetic interactions can also be increased by pleiotropy, or the number of reactions associated to a particular gene. Yeast 5 has an 8.43% increase in the mean number of reactions per gene compared to iMM904. The most prominent example is ISC1, a gene important in sphingolipid metabolism. ISC1 is included in annotation for 60 reactions in Yeast 5, but only 18 in iMM904. ISC1 accounts for a 1% increase in pleiotropy by itself. FOX2, a multi-function enzyme involved in beta-oxidation, ranked highest for pleiotropy in iMM904 with 23 reactions. It is also associated with 23 reactions in Yeast 5. Additional trends in positive or negative epistasis across different types of mutations can also be observed for these models ( Additional file
Generic demand reactions in yeast 5 - towards a functional biomass definition
The yeast consensus reconstruction draws upon data sources with varying levels of compound specificity. For example, the KEGG database includes a general representation of yeast sphingolipid metabolism, while recent suggestions for changes to Yeast 4 include more specific chemical species
Where "isa" reactions are reversible, they can introduce unrealistic interconversion of metabolites. For example, since octanoate is a fatty acid and hexadecanoate is a fatty acid, reversible "isa" reactions in a model would create a nonrealistic pathway by which octanoate could be converted to hexadecanoate via the "isa" reaction, instead of through biochemical pathways which have been documented
An unanticipated result of this approach to varying levels of biochemical specificity in the model is that "isa" reactions effectively embed the logical OR into the model. Thus, where the biomass definition includes "lipid" as a required component, this objective function can be satisfied by any of the compounds that can be converted to "lipid" via an "isa" reaction (Figure
Figure 1
Using "isa" reactions
Using "isa" reactions. Yeast 5 uses "isa" reactions to encapsulate specific chemical species within more general classes. For example, A) the specific species inositol-P-ceramide-A "isa" inositol phosphoceramide (IPC). In turn, IPC "isa" complex sphingolipid. B) Complex sphingolipids participate in the stoichiometrically constrained reaction which produces the species "lipid". C) The lipid species is a component of biomass. This hierarchical model structure embeds logic in the biomass definition: biomass consists of L-alanine AND phosphatidylcholine AND (inositol-P-ceramide-A OR Inositol-P-ceramide-B OR any of the 88 other complex sphingolipids included in the reconstruction). A model user is free to constrain the fluxes which produce specific complex sphingolipids to model an observed lipid composition, or may leave the model unconstrained if the more general biomass definition is sufficient for their needs
Continuing efforts to reconstruct yeast metabolism - an invitation for continued community involvement
Computational reconstruction and modeling of yeast metabolism is an ongoing project. Suggestions for improving the yeast consensus reconstruction or derived models should be submitted to firstname.lastname@example.com. Metabolites and enzymes should be unambiguously identified, using existing model or database (ChEBI or UniProt) identifiers. New reactions should be supplied with primary evidence for their mechanism and catalysis, via PubMed identifiers. Reactions without evidence should have clear reasons for their proposed addition.
We also invite researchers to submit models derived from the yeast consensus reconstruction for hosting at
The Yeast 5 expansion of the Yeast Consensus Reconstruction refines the computational reconstruction of yeast metabolism and improves the predictive accuracy of a stoichiometrically constrained yeast metabolic model. It refines the biochemical reactions included in the reconstruction, particularly reactions involved in sphingolipid metabolism; updates gene-reaction annotations; and emphasizes the distinction between reconstruction (GENRE) and stoichiometrically constrained model (GEM). This update also improves the accuracy of model prediction of viability and auxotrophy phenotypes and increases the number of epistatic interactions. Yeast 5 differs from previous reconstructions and models by emphasizing the distinction between the yeast metabolic reconstruction and the stoichiometrically constrained model, and makes both available as Additional file
Though Yeast 5 consists of a more complete reconstruction and more accurate model of yeast metabolism than previous efforts, the goal of building a complete and accurate computational reconstruction of yeast metabolism must remain an ongoing community effort. As Yeast 5 limitations are identified, they provide opportunities for continued research to improve the computational reconstruction and simulation of the yeast biochemical network.
Yeast 5 scope
The scope of Yeast Consensus Reconstruction was originally determined by the data sets used for its construction: the iMM904
Deriving reconstruction (GENRE) from model (GEM)
We must maintain a distinction between the
Yeast model conventions
The Yeast 5 GEM includes conventions to facilitate model analysis using the COBRA Toolbox, such as standardized representation of exchange reactions, though such conventions are not required in the SBML specification. However, we have chosen to rely upon the SBML standard for encoding reaction and metabolite annotation, rather than the nonstandard custom notes field currently used by the COBRA toolbox. This information, which includes metabolite ChEBI identifiers and literature references supporting reaction inclusion, is encoded in the model .sbml file and is available to MATLAB users via the SBML toolbox. An additional model convention is that the Yeast 5 GEM includes biomass as a species in the model to help make our approach to simulating biomass production more explicit.
Exchange reactions in the Yeast 5 GEM follow a convention of using compounds in the model as reactants, leading to exchange reactions of the form "reactant ->", with an entry of +1 in the stoichiometric matrix. Thus, positive flux values for exchange reactions represent compounds produced in FBA simulation, and negative flux values represent compounds consumed. Since reactions must include both substrate and product in SBML, we have followed the COBRA toolbox convention of denoting exchange reaction species which lay outside the model with the subscript "_b". These species are not loaded into the COBRA toolbox data structure, and serve only as placeholders for exchange reaction substrates.
We have chosen to include biomass as a species in the Yeast 5 GEM. Thus, when conducting FBA on the Yeast 5 GEM, the biomass exchange reaction can be selected if biomass optimization is the desired objective function. For modeling purposes, the species "biomass" is produced in the Yeast 5 GEM via a reaction which consumes 37 biomass precursor compounds to produce biomass, ADP, protons, and phosphate. The biomass precursors include water, polysaccharides, nucleotides, amino acids, riboflavin, sulfate, and the general species "lipid". The "lipid" species in the Yeast 5 GEM serves a function similar to Zanghellini et al.'s "virtual membrane particle"
Constraining reactions in the yeast 5 GEM
The Yeast 5 GEM has over 300 more directionally constrained reactions than Yeast 4. Such constraints incorporate thermodynamic information into the GEM, and often serve in part to limit Type III cycling, which arises from the linear programing approach, but are thermodynamically infeasible
Simulating yeast growth
The Yeast 5 GEM includes 170 exchange reactions, each of which defines a compound that can be included as a medium component for simulation purposes. As distributed, the simulated media is a glucose-limited minimal aerobic medium with constrained limited uptake of glucose, and unconstrained exchange of oxygen, ammonium, protons, iron(2+), phosphate, potassium, sodium, sulfate and water. To simulate anaerobic growth, the oxygen exchange reaction may be constrained to disallow oxygen uptake. Simulating anaerobic growth also requires that the simulated media be supplemented by allowing exchange of ergosterol, lanosterol, zymosterol and phosphatidate, and the biomass definition be changed by removing 14-demethyllanosterol and ergosta-5,7,22,24(28)-tetraen-3beta-ol from the "lipid" definition. These requirements reflect the observation that yeasts require sterols
Growth simulations were performed using the COBRA toolbox
Gene deletion simulation
Gene deletion simulations were performed using the COBRA toolbox
Simulation of genetic interactions
Genetic interactions were quantified with the nonscaled multiplicative definition of epistasis, ϵ = Wxy-WxWy
Additional file 10
Table S5. Gene interaction analysis.
Click here for file
MATLAB Scripts
modelToReconstruction.m: A script which can be used to generate the Yeast GENRE from the Yeast GEM; testYeastModel.m.m.; A script which can be used to compare various yeast GEMs (tested with iND750, iMM904, Yeast 4, and Yeast 5); fluxDistribution.m: A script demonstrating aerobic and anaerobic growth simulations, and comparing the fluxes through glycolysis, the TCA cycle, and the pentose phosphate pathway.
SBML network files
yeast_5.01_model.xml: The Yeast GEM; yeast_5.01_recon.xml: The Yeast GENRE.
MATLAB Scripts
model To Reconstruction.m, A script which can be used to generate the Yeast GENRE from the Yeast GEM; test Yeast Model.m, A script which can be used to compare various yeast GEMs (tested with iND750, iMM904, Yeast 4, and Yeast 5); flux Distribution. m, A script demonstrating aerobic and anaerobic growth simulations, and comparing the fluxes through glycolysis, the TCA cycle, and the pentose phosphate pathway; yeast_5.01_model.xml, The Yeast GEM; yeast_5.01_recon.xml, The Yeast GENRE.
SBML network files
model To Reconstruction.m, A script which can be used to generate the Yeast GENRE from the Yeast GEM; test Yeast Model.m, A script which can be used to compare various yeast GEMs (tested with iND750, iMM904, Yeast 4, and Yeast 5); flux Distribution. m, A script demonstrating aerobic and anaerobic growth simulations, and comparing the fluxes through glycolysis, the TCA cycle, and the pentose phosphate pathway; yeast_5.01_model.xml, The Yeast GEM; yeast_5.01_recon.xml, The Yeast GENRE.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
BDH contributed sphingolipid updates to the GENRE, added constraints to improve GEM flux predictions, compiled the essential and auxotroph-inducing gene lists, and wrote the manuscript. KS conducted the FBA for model evaluation and comparison, wrote the Matlab scripts included as supplemental data, and maintains the Yeast SBML and database files. BB conducted the epistasis analysis. PM leads the Computational Systems Biology & Biochemical Networks Modeling Group at the University of Manchester. LPW leads the Biomass Conversion Group at Cornell University. All authors have contributed to writing and have approved the manuscript.
Thanks to Tunahan Cakir, Nicolas Le Novere, Chuan Lu, Hanan Messiha, Naglis Malys, Michael Howard, and Neil Swainston for submitting suggestions which have been incorporated in Yeast 5.
BDH's work in the Cornell Biofuels Research Laboratory was supported by U.S. Dept. of Transportation, Federal Grant #DTOS59-07-G-00052, and the U.S. Department of Agriculture, Award #2010-38502-21900. KS and PM acknowledge the support of the BBSRC/EPSRC grant BB/C008219/1 "The Manchester Centre for Integrative Systems Biology (MCISB)". Work in the PM lab is also funded by EU Framework 7 grants UniCellSys (201142) and BioPreDyn (289434).
Genome-Scale Reconstruction of the Saccharomyces cerevisiae Metabolic Network
Genome-scale models of microbial cells: evaluating the consequences of constraints
Reconstruction of biochemical networks in microorganisms
KEGG for representation and analysis of molecular networks involving diseases and drugs
Expansion of the BioCyc collection of pathway/genome databases to 160 genomes
Fifteen years of large scale metabolic modeling of yeast: Developments and impacts.
The Saccharomyces Genome Database Project: Saccharomyces cerevisiae S288C genome annotation: a working hypothesis
A consensus yeast metabolic network reconstruction obtained from a community approach to systems biology
Reconstruction and validation of Saccharomyces cerevisiae iND750, a fully compartmentalized genome-scale metabolic model
Metabolic functions of duplicate genes in Saccharomyces cerevisiae
The genome-scale metabolic model iIN 800 of Saccharomyces cerevisiae and its validation: a scaffold to query lipid metabolism
Connecting extracellular metabolomic measurements to intracellular flux states in yeast
Reconstruction annotation jamborees: a community approach to systems biology
Social engineering for virtual "big science" in systems biology
A community effort towards a knowledge-base and mathematical model of the human pathogen Salmonella Typhimurium LT2
Further developments towards a genome-scale metabolic model of yeast
Metabolic modelling of microbes: the flux-balance approach
Adding semantics in kinetics models of biochemical pathways.
Mathematical models of cell factories: moving towards the core of industrial biotechnology
Evaluating Sphingolipid Biochemistry in the Consensus Reconstruction of Yeast Metabolism
Stoichiometric network reconstruction and analysis of yeast sphingolipid metabolism incorporating different states of hydroxylation
ChEBI: a database and ontology for chemical entities of biological interest
The systems biology markup language (SBML): a medium for representation and exchange of biochemical network models
Applications and trends in systems biology in biochemistry
SBMLToolbox: an SBML toolbox for MATLAB users
Quantitative prediction of cellular metabolism with constraint-based models: the COBRA Toolbox v2.0
Global analysis of the yeast lipidome by quantitative shotgun mass spectrometry
Saccharomyces Genome Database provides mutant phenotype data
An integrated approach to characterize genetic interaction networks in yeast metabolism
Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis
Large-scale 13 C-flux analysis reveals mechanistic principles of metabolic network robustness to null mutations in yeast
Modular epistasis in yeast metabolism
Multiple knockout analysis of genetic robustness in the yeast metabolic network
Plasticity of genetic interactions in metabolic networks of yeast
Prevalent positive epistasis in Escherichia coli and Saccharomyces cerevisiae metabolic networks
Epistatic Interaction Maps Relative to Multiple Metabolic Phenotypes
The human metabolic reconstruction Recon 1 directs hypotheses of novel human metabolic functions
Model-driven analysis of experimentally determined growth phenotypes for 465 yeast gene deletion mutants under 16 different conditions
Genome-wide screen for inositol auxotrophy in Saccharomyces cerevisiae implicates lipid metabolism in stress response signaling
A protocol for generating a high-quality genome-scale metabolic reconstruction
Dynamic Epistasis for Different Alleles of the Same Gene.
An expanded genome-scale model of Escherichia coli K-12 (iJR904 GSM/GPR)
Reconstruction and Validation of RefRec: A Global Model for the Yeast Molecular Interaction Network
Quantitative modeling of triacylglycerol homeostasis in yeast - metabolic requirement for lipolysis to promote membrane lipid synthesis and cellular growth
Energy balance for analysis of complex metabolic networks
Flux balance analysis: A geometric perspective
Systematic assignment of thermodynamic constraints in metabolic network models
Sterol structure and membrane function
Initiation of anaerobic growth of Saccharomyces cerevisiae by amino acids or nucleic acid bases: ergosterol and unsaturated fatty acids cannot replace oxygen in minimal media
Lipids and their Metabolism


text:
Return to Afflecks Palace and 1989
It's quite a few years since I visited Afflecks Palace – more than I care to remember. It's an alternative clothing/lifestyle shopping emporium in Manchester. On a recent visit to that city, I decided to take a trip down nostalgia lane to see just how rosy life was back then.
Of course, the exterior of the building hadn't changed at all so that bode well for my return. Would I come back out bedecked in tartan trousers, spikey hair and some new piercings? Would this be a return to my younger days or a descent into a mid-life crisis? Oh Jeez, not another one! 🙂
The view of the exterior offered much – a tall Babel-like tower s-t-r-e-t-c-h-i-n-g into the sky, filled with windows promising to metamorphose all who entered into superior beings of coolness, street cred, individuality and psychedelic personas. Well, maybe I exaggerate a little. But at the very least, the clothes, accessories and experience would make us as angry as fucking hell – or maybe just as angry as a punk needed to be in the 80's.
However, if the many intolerant people (bastards) I met during my misspent youth taught me anything, it was that you should never judge a book by its cover. And I guess the same is true of architecture. While it was indeed Afflecks Palace on the outside, the interior was a sort of watered down, homogenised version of the original. The edges were there but time had worn them down a little smoother.
Still, the stairway promised a place of no bullshit and lots of peace and love. So, if you cannot stand confidently on each of these steps, then you'd just better stay outside – or as we used to say back in 1987, you'd just better fuck off! 🙂
I climbed them with the same sense of nervous excitement as I had back in those days.
The shopping in Afflecks is a funny (as in strange – not haha) experience. The layout that we are used to with high street shopping is dispensed with, as space is at a premium. So everything is kinda topsy turvy and on top of each other. One minute you can be browsing some vinyl and then find yourself confronted with a rail of corsets and basques.
Continuing upwards, I was greeted with this barrage of posters along the staircase. The same scene greeted me the last time I visited. Yea, there were some new posters but many of the older bands were still represented on the walls – Joy Division, New Order, some band declaring Anarchy in the UK, The Cure and The Velvet Underground. Unfortunately, the wall also had posters advertising Michael Jackson and some boy band!
My journey in search of nostalgic awesomeness continued onwards and upwards. As it did for many others. There was an equal mix of locals and tourists, the latter looking somewhat perplexed and, for the way out.
I was kinda surprised at how "ordinary" some of the people there looked. Where was the individuality, the independent thinkers, those who sought out differences, the avant-garde?
Everyone looked just so "boring". Maybe they were in disguise and as soon as I reached the top floors, the madness would start to creep in and they would lose their loafers and padded jackets.
But honestly, by this time I had realised that the Afflecks Palace I knew had long since disappeared – that it was more Afflecks Cabin or maybe Afflecks Two-up Two-down. But my quest for some semblance of my lost youth continued – onwards and upwards and upwards … past the posters … past the people in their padded jackets …
… past the badges. Do people still wear these? (I have 2 – on a jacket that doesn't fit me anymore) NOTE TO SELF: The diet starts Monday.
These ones were mostly of bands/singers I didn't recognise, quotes that I didn't understand – oh wait, there one of The Cure, The Flaming Lips and Pink Floyd. Aaaaghhh – Piiinnkk Flloooyydd. #drools ala Homer Simpson. I continued …
… round and round and up the spiralling steel staircase. Past more posters, past more people in loafers, and onwards towards the black and white portraits of anonymous people that implored us to stop hate crime. Just the hate crime? I guessed they were clients or perhaps business owners in the building or maybe just celebrities. But then, I caught a glimpse of my reflection in one of the photos and realised we don't need anyone else to tell us to stop hate crime.
Eventually, I reached the top floor and something resembling the Afflecks Palace that I knew started to emerge. A barber/hairdresser giving alternative haircuts in between all the regular short back and sides.
Although surrounded by the warm and shiny trappings of the herd mentality lifestyle, there was still those breaking the mould, reaching deeper inside themselves and pushing what they found into the faces of all of the conforming, tracksuit bottom/leggings wearing, brand loyal users clones out there. Wow – that's a bit of a MOUTHFUL.
It must be time for my medication.
And across from them were the shops that sold everything from guitars and pink Docs to flat caps, top hats and aviator glasses -yes, aviator glasses!. One of those shops where you could find anything … but never quite the item you were looking for. 🙂
… while on the other side of the counter lay these two jackets, adorned with chains, safety pins and bizarrely, badges of The X Factor and an alsatian dog. Wild guess here but these are probably not authentic! 🙂 They were only cool because of the fan in the background keeping them like that.
And anyways, no self-respecting goth or punk ever hung their jacket on a clothes hanger … beside wellies.
But the rubber was still there, albeit not the rubber you could have fun with. More the rubber your kids could have fun with, although I doubt they'd have the patience to climb that steel staircase to the top floor. Still, tackiness was always a vital element to this place.
But there were still little gems to be found – like The Fox Fairy. A small store full of lovely and unique handmade items including jewellery, clothing and art prints.
I love this shot of this girl peeking out from this quirky shop. I don't know if she's the owner, but I think she should be. Anyone know her?
… and this place! I'm not quite sure what was being sold, if indeed anything was. But it didn't matter.
Afflecks Palace was as much about browsing and discovering – discovering places where there was a jumble of bric-a-brac and guitars, and an X on the floor and chairs covered with graffiti to sit on, and fluffy toys with long yellow legs, and post-it notes telling me about strangers like Ruby McCann who loves John, and a jelly bean tree and friendly people relaxing and reading – far above the street below, above all the grey tracksuit bottoms, above the leggings, above the Arndale centre, above the couple screaming at each other, above the drunk sleeping in front of the cafe, above The X Factor, above the sexism and racism, above the brand loyalty, above the people in the loafers and padded jackets, above the rubber, above the city – just above.
And it made me realise that being alternative was much more than the look, the clothes or the accessories. It certainly wasn't about 30 year old slogans on poxy yellow t-shirts, or Punk'd Image branded carrier bags or haircuts or rubber or chains or pink Docs or badges or corsets or peace or love or where you live or how many times you use the word fuck, or what you are against or what you are for.
And therein lies my dilemma. Alas, I no longer know what being alternative is about. Is it even possible to be alternative nowadays? Is there such a thing? Was there ever?
So, instead of leaving you with some "And Finally" words of wisdom like Jerry Springer would, I'm actually looking for you to tell me, what you think alternative is, and if an alternative even exists?
Humour me – leave a comment, share or Like this post. Thanks for reading and enjoy your evening.
Click on any image below and use the arrows to scroll through the gallery. Feel free to share or leave a comment.
[Not a valid template]
- yuzo_related_post_metabox:
- a:3:{s:17:"yuzo_include_post";s:0:"";s:17:"yuzo_exclude_post";s:0:"";s:21:"yuzo_disabled_related";N;}


text:
|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|"Uwe Schindler (Commented) (JIRA)" <j...@apache.org>
|[jira] [Commented] (LUCENE-3738) Be consistent about negative vInt/vLong
|Sat, 17 Mar 2012 14:09:38 GMT
[ https://issues.apache.org/jira/browse/LUCENE-3738?page=com.atlassian.jira.plugin.system.issuetabpanels:comment-tabpanel&focusedCommentId=13231978#comment-13231978 ] Uwe Schindler commented on LUCENE-3738: --------------------------------------- {quote} bq. The check is only ommitted in the unrolled loop, the for-loop still contains the check. I'm confused... I don't see how/where BufferedIndexInput.readVLong is checking for negative result now...? {quote} I mean that the actual "if (b & mask != 0)" is also in the original while loop. The original while loop then simply proceeds with reading bytes util the highest bit is null. The unrolled loop behaves different (and thats a real bug), because it will silently not read those remaining bytes, so the file pointer is on a different byte after the call. This also affects readVInt!!! In my opinion, we should unroll *all* readVInt/readVLong loops so all behave 100% identical! And in the case of the last byte read (where the current assert is), throw exception. If we don't unroll all readVInts we have to somehow also make the loop exit after too many bytes are read, which would be an costly extra check in the loop - thats the reason why I want to unroll all loops to fail after 5 or 9 bytes. > Be consistent about negative vInt/vLong > --------------------------------------- > > Key: LUCENE-3738 > URL: https://issues.apache.org/jira/browse/LUCENE-3738 > Project: Lucene - Java > Issue Type: Bug > Reporter: Michael McCandless > Assignee: Uwe Schindler > Fix For: 3.6, 4.0 > > Attachments: LUCENE-3738.patch, LUCENE-3738.patch, LUCENE-3738.patch > > > Today, write/readVInt "allows" a negative int, in that it will encode and decode correctly, just horribly inefficiently (5 bytes). > However, read/writeVLong fails (trips an assert). > I'd prefer that both vInt/vLong trip an assert if you ever try to write a negative number... it's badly trappy today. But, unfortunately, we sometimes rely on this... had we had this assert in 'since the beginning' we could have avoided that. > So, if we can't add that assert in today, I think we should at least fix readVLong to handle negative longs... but then you quietly spend 9 bytes (even more trappy!). -- This message is automatically generated by JIRA. If you think it was sent incorrectly, please contact your JIRA administrators: https://issues.apache.org/jira/secure/ContactAdministrators!default.jspa For more information on JIRA, see: http://www.atlassian.com/software/jira --------------------------------------------------------------------- To unsubscribe, e-mail: email@example.com For additional commands, e-mail: email@example.com
|View raw message


text:
|Next book >>
The Brass Ring(2003)
(The first book in the Caitlin Calloway Mystery series)
A novel by Mavis Applewater
Mavis Applewater's debut novel The Brass Ring is back. In the first Caitlin Calloway Mystery, med student Jamie Jameson meets a sexy policewoman Caitlin Calloway who turns her world upside down. The trouble begins when the relationship falls apart. Twelve years later the two are reunited. Haunted by the past and a danger that neither could have imagined they fight to find the love the have lost while fighting to stay alive.
Similar Books by other authors...
Run to You
(Puppy Love, book 2)
Georgia Beers
Weeping Walls
Gerri Hill
The Liberators of Willow Run
Marianne K Martin
What Matters Most
Georgia Beers
Used availability for Mavis Applewater's The Brass Ring
See all available used copies of this book at Abebooks UK or Abebooks US
Paperback Editions
May 2003 : USA Paperback
|Title: The Brass Ring
Author(s): Mavis Applewater
ISBN: 0-9741034-5-4 / 978-0-9741034-5-7 (USA edition)
Publisher: Limitless Corporation
Availability: Amazon Amazon UK Amazon CA
April 2003 : USA Paperback
|Title: The Brass Ring: Volume 1 (A Caitlin Calloway Mystery)
Author(s): Mavis Applewater
ISBN: 1-5087-1703-6 / 978-1-5087-1703-4 (USA edition)
Publisher: CreateSpace Independent Publishing Platform
Availability: Amazon UK Amazon CA
Kindle Editions
March 2015 : USA, Canada, UK Kindle edition
|Title: The Brass Ring (A Caitlin Calloway Mystery Book 1)
Author(s): Mavis Applewater
Availability: Amazon Amazon UK Amazon CA
Genre Pages


text:
- Description
JMI Piggy-Back Camera Mount w/ Quick Release for 8" to 11" SCTs
Do you have and digital camera and an 8" to 11" SCT telescope? Are you interested in combining the two to do some awesome night sky imaging? Then you need to check out the JMI Piggy-Back Camera Mount w/ Quick Release.
It connects, in most cases, with the screws that are already present in the telescope's optical tube. A three-point quick-release design allows for quick removal and reinstallation with automatic realignment. Simply reinstall the bracket with a single thumbscrew and you are ready to go. Then just add a camera... and clear, dark skies!
JMI Product Number: BRKTPIGQR
- Additional Information
Additional Information
SKU JM-BRKTPIGQR Manufacturer JMI Warranty 1 Year Warranty
- Reviews
- Included Items
Included Items
- JMI Piggy-Back Camera Mount w/ Quick Release for 8" to 11" SCTs
- Questions & Answers
Product QuestionsNo questions yet. Be the first to ask the question!
- Support / Downloads


text:
In this shocking revelation, Personal Identification Numbers for millions of bank cards have been openly published.
To my horror, mine is listed in there. Is yours?
Check out http://www.positiveatheism.org/crt/pin.htm
This is transferred to the new board at http://fraudreports.prophpbb.com
Red Dwarf wrote:To my horror, mine is listed in there. Is yours?
trobbins wrote:What about ATM's that do not hold on to the card until the transaction is finished?
All the ATM's I have ever used, you just put the card in and remove it.
Red Dwarf wrote:I have had no experience of any ATM that does that - in fact there aren't any in this country.
Users browsing this forum: No registered users and 1 guest


text:
It only takes a minute to get started. Fill out our simple sign up form and login to the site.
This step is up to you but will dramatically boost your chances of getting interest from other over 40s members on the site. Let people know a bit about yourself, your pasttimes and upload your nicest photo of yourself.
Use our website's state-of-the-art search to locate over 40s members who match your requirements. Or just browse through listings and locate members that pique your interest.
Discovered someone who could be a good match? Send an email to them or just a wink and let it develop from there. With a little luck you'll be on your way to discovering your ideal partner.
It's quick and easy


text:
I now come with a badass scar on my forehead. PSA: don't bike and text.
Recently working on perfecting roast chicken amongst other culinary endeavors. Next up is most likely ramen.
Trying to get myself regularly running 5k so I can force myself to run 10k, i.e. masochism.
FaceTiming my 11-month old niece so she remembers who I am and forgets my brothers.
TV: Game of Thrones, Six feet Under, The Wire, Mad Men, Curb Your Enthusiasm, The Adventures of Pete and Pete
Music: Mars Volta, The Shins, Blonde Redhead, The Beach Boys, Beatles, Beck, Bjork, Dylan, Flaming Lips, Buddy Holly, Bowie, Elliott Smith, The Kinks, The Zombies, Nat King Cole
Movies: Chinatown, Pulp fiction, There Will Be Blood, The Big Lebowski, Manchester by the Sea, Rushmore, Requiem for a Dream, Labyrinth
Food: Nom nom nom.


text:
IBM Announces Phase Change Memory- 100x More Efficient
IBM announced today a special breakthrough in computer memory tech that may lead to solid state drives being able to store as much as NAND flash technology but work at a speed of 100x faster than current clock speeds, and have a much longer lifespan.
The technology is based on phase-change memory chips that store two bits of data per cell, forgoing corruption problems (a previous setback in PCM technology).
While IBM wasn't the first to discover this new technology, it is important that they are working separately from NEC on the project.
The new memory retains data even when the power supply is shut down, this means you won't get any system errors after you reboot from a power outage.
Now, let's do some comparisons here. Where as regular NAND flash typically gets 5,000 to 10,000 write cycles in memory, PCM can sustain 5 million write cycles, automatically justifying any expensive cost in purchasing the memory.
What's even cooler is that PCM can be used as an extension to DRAM (the current type of memory found in most computers). This will allow us to keep the RAM we currently have and install some PCM in the extra memory slots we have (and with the appropriate motherboard, of course).
The development of PCM is extremely important as DRAM is hypothesized to hit a virtual wall in the next couple years based on how we can elevate the current technology. The only major drawback I see is that PCM will cost at least 5x the price of regular RAM, at least initially.
Photo Courtesy of engadget
Photo Courtesy of underactive


text:
#IHD16 Rookie
In mid-August I received an email from Katie Pratt (Illinois Farm Girl) about attending the Illinois Harvest Dinner. To say the least, I was ECSTATIC! I was at school and couldn't click reply fast enough. But then I waited to reply because I didn't want to comes off as desperate. Yes, I was THAT girl. I casually replied a couple hours later and managed to contain my excitement. Katie co-chairs the event alongside Mary Mackinson Faber from Mackinson Dairy Farm….


text:
A couple of weeks back I was lucky enough to attend an incredible launch with Maybelline, not only have they got heaps of new products coming out (more information on those will come soon), but they also launched their new slogan and initiative 'Make It Happen'. Gone are the 'Maybe She's Born With It' days, Make It Happen is all about empowering women and young girls with the confidence to achieve anything that they set their mind too. For this new launch they are looking for six kiwi girls who would act as ambassadors for the brand with Maybelline and Miss FQ, supporting them to achieve their dreams in whatever field they are passionate about. Read more below for the information on why and how you should enter, plus I have an amazing giveaway of some of the new their lip range too to celebrate this momentous change.
One thing I love doing is treating myself to a good face mask as a bit of pampering and relaxation for my skin. I try to do one at least once a week, sometimes even twice a week depending on how my skin is doing and whether it needs some extra attention. After the hype of the Manuka Doctor ApiClear Purifying Facial Peel Mask* (NZD $35.95), which I absolutely loved and have since finished, I was intrigued to try some of their other masks. The ApiClear Purifying Face Mask* is from the same range as the Facial Peel - aimed at troubled skin/acne prone/oily skin.
The latest Lust Have It Women's Beauty Box* is here with the theme of Winter Skincare - inside are some products to keep the skin hydrated in this chilly weather. If you missed it, you can see what was inside the May box here, and if you are new to these parts, Lust Have It is an Australian and New Zealand monthly subscription service where you are sent at least 5 products that range from full size to sample sizes from a variety of brands. It's a fun way to be introduced to new products and try things you may have otherwise not.
Just about every brand now are bringing out some sort of contouring and highlighting kit. It seems us beauty lovers can't get enough of them, I know I am a big fan of them and have been loving trying out the different offerings. UK based brand Collection have brought out their version* to the market, at a great price of NZD $21.99. But what's the quality like...?
I always get so excited when a brand like L'Oreal drops a whole lot of new product for us NZ beauty lovers to indulge in. In May I went along to a gorgeous brunch at Gusto at Sky City Grand and got to hear all about the new products launching and we were given a demonstration of the products by NZ makeup artist Lisa Matson who has been in the industry for over 20 years. She talked us through the new products as she applied them, and I couldn't wait to get home to try them for myself.
You could have all the expensive makeup in the world, but it's not going to apply well if you haven't got the right tools. A good makeup brush will do wonders to how product applies, and looks on the skin. The Beauty Collective through My Beauty Store have brought out the Simply Essential Pro Series Makeup Brushes* that are affordable, but high quality so you can get the most out of your makeup.
There's nothing like a good eyeshadow palette to get me excited! I am eyeshadow palette obsessed and have way too many for any normal person, but I just always love finding and adding new ones to my collection. Wearing eyeshadow on the daily means I've used my fair share of various brands, from the affordable to the expensive. Mellow Cosmetics, a NZ owned and operated company, offer this large Glam Squad Nude Beauty Palette*, which I received in the Best Beauty Box Ever Incredible Eyes Box* (reviews here). In that post I promised that I would review this palette more in-depth as it's kind of outstanding for the price!
You might know Ardell for their false lashes, but now they have branched out to offer more cosmetics products in the form brow products. They've recently come out with a host of new brow bits so they are always looking #onfleek, with a pomade, brow kit, and brow gels. Read on to hear my thoughts on some of these new products.
I'm not a huge jewellery wearer - what a way to start a post that is obviously all about it but bear with me for a second! Despite this, I do like collecting special items that have a lot of meaning to them and wear them on occasion. I also like the idea of handing down precious items; I have some lovely pieces from my Mum, that have been passed down through the generations that I treasure. Soufeel, based in the USA, reached out to me asking if I wanted to put together one of their charm bracelets, allowing me to pick my own charms and make my very own personalised photo charm, or Memorable Charm as they call them. I love this style of charm bracelet, so was keen to try out the website and see what the quality of their products was like.


text:
AFTER being made redundant twice in three months, for 25-year-old Anna Ironside the prospect of owning her own shop seemed almost impossible.
Last month, that dream became a reality though and she opened her own interiors shop, Anna Florence, which is already proving popular in Malton.
Decorated in the style of an apothecary, the shop, based in Market Street, specialises in vintage and natural interiors, all chosen by Anna, some of which she has found hard to part with. "One of my favourite pieces is the bureau display in the window at the moment," she said. "People have to remind me that I do have to sell it all because pieces come in and I just want to keep them."
Anna, who is originally from Scarborough, is undertaking a course in interior design and is looking for local artists whose work she could feature in her shop.
She said: "I'd like to feature the work of more local people. I stock some well-known names, such as Fired Earth paints, as well as encouraging up-cycling, when an everyday product can be made in to something elegant and useful."
The ambitious interior designer will also launch the shop's website soon at www.annaflorence.com, and free delivery and collection is offered in the Malton area.
She said: "It's all very exciting and a bit scary at the moment because it has happened all of a sudden, but everyone has been really positive."
Phone Anna on 01653 696185.