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How to Buy a Back-to-School Desktop
A new computer is almost a necessity these days, particularly when you're buying it during for one of your favorite student's transitions (from middle school to high school, or high school to college, etc.). While laptops have their place, particularly in upper education, your student might want a desktop instead. For example, she might already have an Apple iPad or netbook for portable computing. She might need a larger screen for engineering projects. She might want to play high-end 3D games during time off. Or she might simply want the theft resistance and extra power that a desktop affords its users. How do you choose?
The first thing to do is check with your school for specific system requirements. Some colleges and universities want their students equipped with a Windows-based PC (desktop or laptop), to cut down on software incompatibility issues. Others will let you use almost any operating system. Many institutions will have on-site repair centers that only service university-bought PCs. And most schools offer price breaks for particular vendors and include extensive software bundles, which can shave off a good amount from your PC purchase.
Form Factor: It's About Your Space
The simplest type of desktop to buy is the all-in-one. The built-in screens range from 17 inches (like in the largest laptops) to over 27 inches. Since most people never crack open their PC's case, the all-in-one desktop PC is a great choice for the student. Small form factor and mini desktop PCs trade expandability for size: They are among the smallest PCs out there. (Note that for all systems aside from the all-in-ones, you'll have to buy a monitor separately.) Small PCs like these tend to have HDMI outputs, so you can plug them into HDTVs, instantly saving you some room. Next up in size are full sized desktops, midtowers, and full workstation-class towers. You'll need larger systems for specialized equipment like multiple hard drives and graphics cards. Full-sized towers are the most expandable. All these extra components like graphics cards and hard drives make sense for graphics art students, architects, and engineers.
Power: More Doesn't Always Mean Better
The desktop you buy for your student should be able to last during the 4 to 6 years he stays in each stage. Dual-core processors from AMD or Intel are a must for all students, but quad- and six-core processors make more sense if science or art are in your educational plan.
Everyoneregardless of majormultitasks these days. While journalism majors may instant message, surf the Web, and write simultaneously, an animation arts student may add CGI 3D rendering to that mix. Heavy-lifting tasks like animation production and CAD drawings are the reasons to buy higher-end processors and graphics cards. Everyone else should manage fine using a lower-powered system like an Apple Mac mini or Gateway DX4840-02e. We'd be amiss if we didn't address gaming: if your student like to play the latest games as they are released, look for a high end card like the ATI Radeon HD 5870 or Nvidia GeForce GTX 460. Everyone else can play casual games fine on integrated graphics like Intel's GMA HD. Higher-end graphics cards and processors correspond to higher prices, though these components' higher performance can temper the price hit.
What Features Matter
You may be tempted to save a few bucks by buying a system without an optical drive. For the time being, don't give in to that temptation. DVDs are still a valid medium for transferring data, particularly if it's packed in with a textbook. Spend a little more on a system with a Blu-ray player, and your student will be able to watch movies and HD films. True, online video will eventually kill optical media, but not yet.
Hard drives should be large, 500GB or higher for most, 1TB or higher if you have an art or film enthusiast in your family. In terms of ports, eSATA, FireWire 800, and USB 3.0 are faster connector standards on external hard drives, though at this point, very few systems ship with USB 3.0 ports, so USB 2.0 is still fine. Choose a desktop with one or all three if your student likes to trade music, photos, or videos. For system memory 2GB should be the bare minimum, and anyone in the sciences or arts should seriously consider four GB or more.
A Word on Warranties
Last, but not least, warranties are an important consideration. Most consumer systems will have a 1-year parts and labor warranty. Costco increases that number to two years for most of the systems it sells. Apple is a notable exception: Macs hold a 1-year parts/labor warranty standard, but only 90-days for initial phone and setup support. After the first 90-days, you'll have to bring your system to an Apple Store for the quickest service. Other PC manufacturers may use in-house support or farm tech support out to a cheap data center somewhere in the world. Check these policies carefully if you're hard on your equipment. In any case, most defects come to the surface within the first year of ownership. After the warranty dries up, you're looking at support for pay. Extended warranties can be purchased for additional fees, up to three-four years in some cases.
When it comes to buying a desktop for school, there's certainly a lot of factors to weigh. We hope our guide has helped give you a clearer idea of what to choose. To get more details on specific desktops, check out our desktop product guide.


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The East China Sea is a typical case 2 water environment, where concentrations of phytoplankton pigments, suspended matter, and chromophoric dissolved organic matter (CDOM) are all higher than those in the open oceans, because of the discharge from the Yangtze River and the Yellow River. By using a hyperspectral semianalytical model, we simulated a set of remote-sensing reflectance for a variety of chlorophyll, suspended matter, and CDOM concentrations. From this simulated data set, a new algorithm for the retrieval of chlorophyll concentration from remote-sensing reflectance is proposed. For this method, we took into account the 682-nm spectral channel in addition to the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) channels. When this algorithm was applied to a field data set, the chlorophyll concentrations retrieved through the new algorithm were consistent with field measurements to within a small error of 18%, in contrast with that of 147% between the SeaWiFS ocean chlorophyll 2 algorithm and the in situ observation.
© 2000 Optical Society of AmericaFull Article | PDF Article
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subset: commoncrawl


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A Division of Vacations To Go
After your wedding, a honeymoon resort in St. Lucia is the perfect place to relax, rejuvenate and start a new life together. Watch a tropical sunset from your room's balcony, soak in a private Jacuzzi or stroll along the area's white-sand beaches with your loved one.
Some of our honeymoon resorts are for adults only, and some cater only to couples. Many resorts offer special honeymoon packages that might include upgrades, late checkout, complimentary chilled champagne, and seaside dinners for two.
The hotels and resorts on this page have been divided into two groups. Immediately below are the all-inclusive St. Lucia resorts, where lodging, meals, activities and more are rolled into one price. Traditional hotels and resorts are listed second.
Mon-Fri 7am to 11pm, Sat 8am to 9pm, Sun 9am to 10pm, U.S. Central Time


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When British Prime Minister David Cameron tweeted a photo of himself speaking to US President Barack Obama, he gave online jokers all the ammunition they needed.
The internet picked up the photo and the barraged of memes ensued. We are sure the PM is wondering what he was thinking.
Here's the tweet:
I've been speaking to @BarackObama about the situation in Ukraine. We are united in condemnation of Russia's actions. pic.twitter.com/7Rk2k8iOIK — David Cameron (@David_Cameron)
March 5, 2014
And this is what has been happening since:
@Glinner Just trying to complain about the funny smell emanating from my phone... #davecalls pic.twitter.com/gk5h2wNYUQ — Official Tim Wood (@OfficialTimWood)
March 5, 2014
You won't believe the filth Mr. Cameron is spouting on this phone call... pic.twitter.com/aXzubsazwU — Andy Hanley (@Hanners1979)
March 5, 2014
Remember this extremely talented dog?
Yes, Dave, this is Dog pic.twitter.com/pbElW856BO — Jon Bounds (@bounder)
March 5, 2014
And the knocker has to be:
David who ? #davecalls pic.twitter.com/JIlOTeocpY — I'm Atticus (@windlerob)
March 5, 2014
All right Chief, I'm in the cone. @David_Cameron @BarackObama @robdelaney #davecalls pic.twitter.com/ZzNzIJNOfU — faex populi (@faexpopuli)
March 7, 2014
.@David_Cameron @BarackObama Hi guys, I'm on the line now too. Get me up to speed. pic.twitter.com/xhmJG5KpxT — rob delaney (@robdelaney)
March 5, 2014
.@YoungAdolf_ @MacMoreno @SirPatStew @robdelaney @David_Cameron @BarackObama Sorry grabbed a snack. Any new intel? pic.twitter.com/ulfohnO2Sn — Matt Quick (@MattTheQuick)
March 6, 2014
Even celebrities couldn't resist:
.@robdelaney @David_Cameron @BarackObama I'm now patched in as well. Sorry for the delay. pic.twitter.com/elLQcKcV3w — Patrick Stewart (@SirPatStew)
March 5, 2014
no. i will not stop. i haven't even started. #davecalls pic.twitter.com/rJrb1LRrTf — Caroline (@CarolineKent)
March 6, 2014
What happens when you cross the memes? #davecalls #businessbaby pic.twitter.com/GgJr7ZU2Nm — Dan Pursey (@danpursey)
March 6, 2014
Oh please. Straight to voicemail. #davecalls pic.twitter.com/Y1PIP1wJ2V — Russell Balkind (@russellbalkind)
March 5, 2014


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Now you have the opportunity to get into the skin of Miners. Vozmite
over the management of special equipment and you look Tonelli and mine
A 3D graphic engine which openly unrealistically represents the event
at every time is used for the realistic representation. Bit by bit the
player gets more experienced and gets special missions by the more
extensive one, these demand whole attention and the dexterity.
A level is regarded as solved if the corresponding tasks were
accomplished within the predefined time. Further events influence the
regular event, such as, again and again: -
- unforeseen obstacle
- influences of weather,
- like mush, ice and rain
- short-term special actions
- technical defects
- Complete, detailed landscape in 3D graphic,
- innumerable tasks wait a gripping, ongoing story
- weather effects and day/night change
- missions with a Highscore function for the player
- by which prolonged game fun - free game after mastering all tasks
- extensive background information to the job outline
- intuitive control by keyboard and mouse
Release: Mining.and.Tunneling.Simulator-1C
Size: 382 MB
NFO: Mining.and.Tunneling.Simulator-1C - Wiki
Audio Bitrate:
[Linkleri görebilmek için üye olun veya giriş yapın.]
[Linkleri görebilmek için üye olun veya giriş yapın.]
[Linkleri görebilmek için üye olun veya giriş yapın.]


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Places to Visit Near Langsa
144 Kms from Langsa
Places to Visit · Getaways · Trips · Hotels
Ko Tarutao
285 Kms from Langsa
This island – located close to the Malaysia border – is said to be one of the most unspoiled islands in Thailand. It was also the setting for the fifth season of the reality TV show Survivor. While not exactly undiscovered it's quiet, clean, beautiful and protected – everything the main tourist islands are not. There are no major resorts, no beach parties just an ample supply of peace and gorgeous natural scenery. It's protected national park status also ensures there is plenty of wildlife – as well being a nestling ground sites for turtles, Langurs, crab-eating macaques and wild pigs are common. Accommodation on the island is run by the National Park and guests can choose from either a tent or a fan-cooled bungalow.
Getaways · Places to Visit · Hotels · Trips
284 Kms from Langsa
Places to Visit · Getaways · Trips · Hotels


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This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
During developmental and adult neurogenesis, doublecortin is an early neuronal marker expressed when neural stem cells assume a neuronal cell fate. To understand mechanisms involved in early processes of neuronal fate decision, we investigated cell lines for their capacity to induce expression of doublecortin upon neuronal differentiation and develop in vitro reporter models using doublecortin promoter sequences.
Among various cell lines investigated, the human teratocarcinoma cell line NTERA-2 was found to fulfill our criteria. Following induction of differentiation using retinoic acid treatment, we observed a 16-fold increase in doublecortin mRNA expression, as well as strong induction of doublecortin polypeptide expression. The acquisition of a neuronal precursor phenotype was also substantiated by the establishment of a multipolar neuronal morphology and expression of additional neuronal markers, such as Map2, βIII-tubulin and neuron-specific enolase. Moreover, stable transfection in NTERA-2 cells of reporter constructs encoding fluorescent or luminescent genes under the control of the doublecortin promoter allowed us to directly detect induction of neuronal differentiation in cell culture, such as following retinoic acid treatment or mouse Ngn2 transient overexpression.
Induction of doublecortin expression in differentiating NTERA-2 cells suggests that these cells accurately recapitulate some of the very early events of neuronal determination. Hence, the use of reporter genes under the control of the doublecortin promoter in NTERA-2 cells will help us to investigate factors involved early in the course of neuronal differentiation processes. Moreover the ease to detect the induction of a neuronal program in this model will permit to perform high throughput screening for compounds acting on the early neuronal differentiation mechanisms.
Neurogenesis relies on a pool of proliferative neural stem cells differentiating into new neurons following induction of a neurogenic gene expression program. The whole maturation process from the multipotent neural stem cells into functional mature neurons involves several factors that orchestrate each step in an intricate continuum. A key event of neurogenesis is the neuronal commitment of multipotent neural stem cells, i.e. the time point at which cells lose their capacity to generate neurons as well as glia, and become restricted to the generation of new neurons.
Doublecortin (DCX) constitutes a marker for neuronal precursors throughout developmental and adult neurogenesis [1-4]. We demonstrated that DCX was expressed very early in newly generated neuronal precursors of the adult CNS [3]. Recently, a cortical subpopulation of multipotent DCX- NG2-expressing cells was reported in the adult CNS [5]. However, the identity of these cells and how they related to neurogenic processes has currently not been elucidated.
Although expression of DCX is well documented in neuronal precursors in vivo and in primary cell cultures, a cell line inducing sustained expression of DCX upon differentiation needed so far to be identified. The identification of such an in vitro model reproducing the early phases of neuronal determination would greatly accelerate the study of factors influencing neurogenesis. NTERA-2 cells have been previously reported to induce a complex neuronal differentiation program in response to retinoic acid (see for examples [6-8]). Based on the induction of DCX and DCX-driven reporter constructs upon differentiation, we further substantiated evidences establishing the NTERA-2 cell line as an appropriate and convenient in vitro model to study early steps of neuronal differentiation and factors acting on this process during development and in the adult CNS.
Five neuronal-like cell lines were tested for their capacity to mimic early events of neuronal determination and differentiation taking place during development or adult neurogenesis: D283 medulloblastoma (human medulloblastoma), DAOY (human medulloblastoma), NTERA-2 (human teratocarcinoma), PC-12 (rat pheochromacytoma), SK-N-SH (human teratocarcinoma). Additionally, HeLa cells (human adenocarcinoma) were chosen as non-neuronal control cell line. To differentiate cell lines towards a neuronal phenotype, retinoic acid (10 μM) was added to the cultures.
Figure Figure11 shows the morphological appearance of cell lines in response to retinoic acid treatment. Hence, after 28 days of differentiation, NTERA-2 cells morphology showed pronounced reorganization characterized by the formation of cell clusters and processes creating a complex cellular network (Fig (Fig1A1A and and1G).1G). In addition, D283 cell lines adopted a flattened morphology and showed increased cell death during differentiation treatment (Fig (Fig1B1B and and1H).1H). Finally, minor or no morphological responses could be detected for DAOY, HeLa, PC-12 or SK-N-SH cells following retinoic acid treatment.
PC-12 cells are known to undergo neuronal morphological differentiation in the presence of nerve growth factor (NGF). To confirm the potential of our PC-12 cultures to adopt a neuronal morphology upon differentiation, we maintained them four weeks in the presence of NGF (50 ng/ml). As previously described, PC12 cells underwent an extensive process outgrowth in response to NGF (Fig (Fig1I1I inset).
To examine if morphological changes observed in response to retinoic acid correlated with expression of genes induced during neuronal early differentiation, expression of DCX was assessed by immunocytochemistry. Figure 2(A–F) shows the absence of DCX expression in undifferentiated cells from the six cell lines investigated. Following differentiation however, robust expression of DCX could be detected in a large fraction of the NTERA-2 cells, especially those forming the cell clusters (Fig (Fig2G).2G). Expression could also be detected at a low level in differentiated D283 cells (Fig (Fig2H).2H). Moreover, most neuronal-differentiated NTERA-2 cells expressing DCX also induced expression of the neuronal-specific microtubule-binding protein Map2, hence revealing some degrees of neuronal maturation (Fig (Fig2G).2G). In contrast, only a few DCX-expressing differentiated D283 cells did express Map2. Finally, Map2 expression could be detected in differentiated PC12 cells and rarely in HeLa cells as well, however, DCX expression could never be detected in these cell lines.
Induction of DCX mRNA expression following retinoic acid treatment was further quantified in total RNA from each cell lines either in a proliferative state or following differentiation. In agreement with observation made by immunocytochemistry, undifferentiated cell lines did not express DCX, or did only in trace amounts (Fig (Fig2M).2M). On the other hand, following retinoic acid-induced differentiation, levels of DCX mRNA in NTERA-2 cells were increased approximately 16 times. Likewise, DCX mRNA levels were approximately 12 times more elevated in differentiated D283 cells then in the non-differentiated cells. The absolute DCX mRNA levels in D283 cells, however, were roughly half of those found in NTERA-2 cells. Finally, DCX mRNA expression was not detected in the other differentiated cell lines.
To examine the induction of DCX expression in respect to other neuronal genes, we followed the expression of NeuroD1, DCX and neuron-specific enolase (NSE) at various differentiation time points, i.e. proliferative state and following 3 days, 7 days, 14 days, 21 days and 28 days of retinoic acid treatment. As shown in figure figure2O,2O, retinoic acid treatment rapidly and transiently induced high levels of NeuroD1 expression, a neurogenic transcription factor [9]. As NeuroD1 expression declined between day 14 and 21 of differentiation, expression of DCX mRNA was induced and further increased until the last time point at 28 days. Finally, expression of NSE mRNA, an enzyme found in mature neurons [10], was induced only after the third week of differentiation. Hence, our differentiating NTERA-2 cultures expressed a key neurogenic factor, a neuronal precursor marker, and a mature neuronal marker in successive phases. This suggests that differentiation of NTERA-2 cells mimicked faithfully the various phases of the neuronal determination and differentiation program also induced in neural stem cells.
Western blot analysis substantiated the induction of DCX expression measured at the mRNA level. Whereas, very low levels of DCX protein expression could be measured in proliferating NTERA-2 cells, strong expression could be detected in differentiated NTERA-2 cells (Fig (Fig2N).2N). In addition, low levels of DCX protein expression could be detected in differentiated D283 cells (Fig (Fig2N),2N), in agreement with the lower abundance of DCX mRNA measured in these cells (Fig (Fig2M).2M). The presence of DCX protein could not be documented in the other cells lines, notwithstanding their differentiation status.
Expression of several neural markers in differentiating NTERA-2 cells was scrutinized in more details by immunocytochemistry at various time points. As shown in figure figure2P,2P, within the first 7 days of exposition to retinoic acid, the expression A2B5, a neural cell surface antigen, was induced in a large proportion of the cells, in agreement with previous report [8]. Expression of nestin, an intermediate filament expressed in neural stem cells, was found in a high percentage of NTERA-2 cells at all time points. Nestin expression, however, decreased slowly during the course of differentiation. In contrast, NG2, an antigen present on the cell surface of glial precursors, could not be detected in our NTERA-2 cultures at any time point.
Expression of neuronal markers became significant after 3 weeks of differentiation. Hence, expression of βIII-tubulin, DCX and Map2 were strongly induced and following 4 weeks of retinoic acid treatment, was found in more then 90% of the cells present in our NTERA-2 cultures. Expression of GFAP was detected in a minor fraction of the NTERA-2 cells at every time point. Finally, expression of NeuN, a marker found in more mature neurons, could not be detected using our differentiation paradigm.
This capacity of differentiating NTERA-2 cells to induce expression of the DCX gene was further exploited to establish an in vitro reporter system for neuronal early differentiation events. We recently reported that a genomic fragment of 3,5 kb of the human DCX gene drove expression of reporter genes in neuronal precursors and young neurons in vitro and in vivo [11,12]. We therefore generated stable NTERA-2 cell lines bearing the EGFP or the firefly luciferase reporter gene under the control of the DCX promoter sequences (NTERA-2DCX-EGFP and NTERA-2DCX-luci). In parallel, we also generated transfected HeLa cell lines (HeLaDCX-EGFP and HeLaDCX-Luci) as non-neuronal controls. Integration of the reporter construct was confirmed by PCR on the genomic DNA (data not shown).
Whereas undifferentiated NTERA-2DCX-EGFP and HeLaDCX-EGFP did not express the EGFP reporter, retinoic acid treatment strongly and specifically induced the expression of EGFP in the NTERA-2DCX-EGFP cells but not in the HeLaDCX-EGFP cells (Fig 3A–H). Immunocytochemistry further confirmed that EGFP-expressing cells also expressed DCX, and frequently Map2 as well (Fig 3I–L).
The induction of the reporter gene in differentiating NTERA-2 cells was examined at the mRNA and protein levels. In agreement with the strong induction of the endogenous DCX mRNA expression following differentiation of NTERA-2DCX-EGFP cells, expression of the EGFP reporter mRNA was observed to be strongly induced (Fig (Fig3M).3M). In contrast, HeLaDCX-EGFP did not upregulated the expression of the EGFP reporter in response to retinoic acid treatment. The potent induction of the reporter's expression in NTERA-2 cells could also be documented by western blotting (Fig (Fig3N).3N). Again, as expected from the mRNA measurements, expression of the reporter was not induced in HeLaDCX-EGFP cells, underscoring the neuronal specificity of the reporter system.
Neurogenin 1 and neurogenin 2 (Ngn2) are transcription factors of the basic helix-loop-helix (bHLH) family expressed in the earliest steps of mammalian cortical neurogenesis. They initiate a cascade of gene activation leading to the expression of terminal neuronal differentiation genes [13,14]. It was recently reported that mouse Ngn2 could bind directly on the DCX promoter and activate it [15]. The capacity of our reporter system to respond to neurogenic factors was tested using a mouse Ngn2-encoding vector to transiently transfect the NTERA-2DCX-luci cells. HeLaDCX-Luci cell were used in parallel as a non-neuronal control. As shown in figure figure3O,3O, brief expression of mouse Ngn2 for as little as four days sufficed to activate DCX-promoter driven expression of the luciferase reporter gene in NTERA-2 cells, but not in HeLa cells.
Over the last few years, DCX became an established marker for neuronal determination and neurogenesis [4,12,16]. In differentiating neural stem cells, either in vivo or in vitro, expression of DCX can be detected upon induction of neuronal differentiation. To date however, no established neural cell line was shown to have the capacity to up-regulate DCX upon differentiation. In this report, we show that NTERA-2 cells in conjunction with DCX reporter constructs constitute an attractive model for the study of early phases of neuronal differentiation occurring during developmental and adult neurogenesis. Although, ultimate validation of factors acting on neuronal differentiation would need to be performed on primary cells, there was a need for such an in vitro system behaving as a simple neuronal differentiation model.
In contrast to other neural cell lines tested, such as PC12 cells, NTERA-2 and D283 cells could induce expression of DCX mRNA in response to retinoic acid exposure, a treatment known to induce a strong neurogenic response (for example Guan et al. [17]). At the protein level however, only differentiated NTERA-2 cells showed significant DCX expression. Expression of DCX in NTERA-2 cells was associated with the establishment of a neuronal morphology, as well as with the expression of other neuronal-specific markers, such as Map2 and NSE.
DCX expression occurs very early during the neuronal determination/differentiation process, possibly due to the direct binding of the bHLH transcription factors such as Ngn2 on the DCX promoter [15]. Hence, it appears that differentiating NTERA-2 cells can mimic neural stem cells in the early phase of neuronal differentiation. Supporting data also come from a recent gene profiling of NTERA-2 cells treated with retinoic acid. The latter revealed an expression pattern compatible with early neuronal phenotypes found in the posterior hindbrain and other posterior CNS regions [18]. Therefore, NTERA-2 cells appear to be well suited to be used as an in vitro model to study the early events of neuronal determination/differentiation and the factors influencing it.
In addition, we showed that genetically modified NTERA-2 cells bearing reporter genes under the control of the human DCX promoter constitute a simple and valuable tool to study factors acting on neuronal determination and early differentiation. We have previously demonstrated that DCX promoter-based expression vectors can drive expression of reporter genes in neuronal precursors and young neurons, both in vitro and in vivo [11,12]. The fact that reporter expression was not induced in HeLa cells further supports the evidence that expression of DCX promoter-based construct in NTERA-2 cells accurately reflects the induction of a complex genetic program similar to the early phase of neural stem cells differentiation into neurons, and not merely a cis-action on the DCX promoter.
We could demonstrate in this report that expression of DCX and DCX promoter-driven reporters occurred in NTERA-2 cells upon induction of neuronal differentiation. Through this new and simple reporter system tool, it will be possible to analyze early events of neuronal determination and differentiation such as occurring during development and adult neurogenesis and identified new neurons directly in statu nascendi. Moreover, this culture model also provides a rapid screening method for molecules influencing the early process of neuronal differentiation.
Cells were maintained in a humidified incubator at 37°C under an atmosphere containing 5% of CO2. HeLa, SK-N-SH, DAOY and D283 medulloblastoma cells were cultivated in MEM(Eagle) with Earle's BSS, supplemented with 10% (v/v) fetal calf serum, 2 mM L-glutamine, 1 mM sodium-bicarbonate, 0.1 mM non-essential amino acids, 100 U/ml Penicillin, 0.1 mg/ml Streptomycin. PC-12 were cultivated in RPMI 1640, supplemented with 10% horse serum, 5% (v/v) fetal calf serum, 100 U/ml Penicillin, 0.1 mg/ml Streptomycin, 2 mM L-glutamine. Finally, NTERA-2 cells were maintained in DMEM, supplemented with 10% (v/v) fetal calf serum, 4 mM L-glutamine, 4.5 g/l Glucose, 100 U/ml Penicillin, 0.1 mg/ml Streptomycin, 1.5 g/l sodium-bicarbonate.
Cells were plated in a T75 culture flask at a density of 1.5 × 104 cells/cm2 in their corresponding media. For the differentiation of D283 and PC-12 cell lines, the culture flasks were coated with poly-ornithine (250 μg/ml) to increase their adherence. One day after seeding, the differentiation was initiated according to a differentiation protocol adapted from [7]. Differentiation was induced by the addition of 10 μM of retinoic acid in the culture media. Cells were maintained under these condition for a period of 2 weeks, during which medium was replaced every 3 days. Thereafter, cells were further cultivated for 2 weeks in their designated medium in absence of retinoic acid. Retinoic acid treatment was previously reported to remodel the genetic program of PC12 cells [19]. Nevertheless, a standard differentiation protocol for the PC12 cells using 50 ng/ml nerve growth factor (NGF) in the culture medium was also applied to confirm their capacity to differentiate into neuron-like cells [20,21].
For stable transfection, cells were plated one day before transfection at a density of 1.5 × 105 cells/cm2 in a T75 culture flask. Vectors encoding the EGFP reporter gene, or the firefly luciferase reporter gene, driven by the human DCX promoter [12] where transfected using Metafectene (Biontex Laboratories, Munich) according to manufacturer's guidelines. Selection of transfected cells began 3 days after transfection using Geneticin (250 μg/ml for HeLa and 300 μg/ml for NTERA-2). After 4 weeks under selection conditions, stable transfected single cells were FACS sorted into 96 wells/plate to establish clonal cultures. In the stably transfected clones, integration of reporter genes was confirmed by PCR on genomic DNA (EGFP-fow GCT GAC CCT GAA GTT CAT CTG, EGFP-rev GGA CTT GAA GAA GTC GTG CTG, Luciferase-fow TCA AAG AGG CGA ACT GTG TG and Luciferase-rev TTT TCC GTC ATC GTC TTT CC, beta-actin-fow AGC CAT GTA CGT AGC CAT CC, beta-actin-rev CTC TCA GCT GTG GTG GTG AA). Vectors used for transfection served as PCR control. Non-differentiated cells expressing the EGFP reporter gene constitutively, as a consequence of an insertion artifact for example, were excluded.
To induce the neuronal differentiation, a vector encoding the complete mouse Ngn2-coding sequence, from ATG to the stop codon, flanked with a Myc tag was transfected using Metafectene (Biontex Laboratories, Munich). We used the bicistronic expression vector pIRES2-DsRed-Express (Clontech) to drive the expression of Ngn2 under the control of the CMV promoter. As control, we used the empty vector pIRES2-DsRed-Express, devoid of Ngn2. On the following day, transfected cell lines were plated in a 96 wells/plate at a density of 5 × 104 cells/well in triplicates in their respective culture media without retinoic acid. Four days after transfection, cells were processed to measure the firefly luciferase activity using the Luciferase Assay System (Promega). Luciferase activities were reported in arbitrary units, with the activities measured in NTERA-2 cells transfected with the empty vector representing the reference (100%).
Gene expression analysis was performed via quantitative RT-PCR on total cDNAs isolated from undifferentiated and differentiated cell cultures. RNAs were isolated using the RNeasy-Kit (Qiagen, Hilden) and cDNAs were synthesized using the RETROscript kit (Ambion, Austin, Texas, USA). Quantitative detection of EGFP transcription product was performed using a Rotor-Gene 3000 real-time PCR apparatus (Corbett Research, Mortlake, Sydney, Australia) and SYBR green master mix (Brilliant SYBR Green, Stratagen). Signals obtained for the EGFP were normalized on the levels of rRNA 15S (RETROscript kit, Ambion). Expression levels are represented as EGFP/rRNA 15S ratio.
For the analysis of neuronal gene expression at various time points of differentiation, total RNAs were extracted from cultures using Tri Reagent (Sigma) according to the manufacturer's protocol. Total RNAs were further purified using the SV Total RNA Isolation System (Promega). Reverse transcription was performed using the Reverse Transcription System (Promega) and controls for each sample were also generated by omitting the reverse transcriptase from the reaction. Real-time PCR was realized using SYBR Green QPCR Master Mix (Stratagen) and a Mx3005P thermocycler (Stratagen). Measurements were normalized on the β-actin mRNA levels and represented for each gene relative to its maximal expression. The following oligos were used: β-actin fow AGC CAT GTA CGT AGC CAT CC; β-actin rev CTC TCA GCT GTG GTG GTG AA; EGFP fow GCT GAC CCT GAA GTT CAT CTG; EGFP rev GGA CTT GAA GAA GTC GTG CTG; DCX fow GGA AGG GGA AAG CTA TGT CTG; DCX rev TTG CTG CTA GCC AAG GAC TG; NeuroD1 fow CCA AAA AGA AGA AGA TGA CTA AGG; NeuroD1 rev AGC TGT CCA TGG TAC CGT AA [8]; NSE fow ATC GCG CCA GCC CTC ATC AGC; NSE rev TTT TCC GTG TAG CCA GCC TTG TCG [8].
Protein expression was analyzed on homogenate derived from non-differentiated or differentiated cultures. Cells were lyzed in SUB buffer (0.5% w/v SDS, 8 M Urea, 2% v/v β-mercaptoethanol) and protein concentrations were determined using the Bradford method (Bradford Reagent, Sigma). Proteins homogenates (3 μg per lane) were separated on a 12.5% polyacrylamide SDS-PAGE and transferred on nitrocellulose membrane for western blotting. Membrane blocking, antibody dilution and washes were performed in the gelatin-western buffer (20 mM Tris-HCl pH 7.3, 0,9% w/v NaCl, 1% v/v gelatin from cold water fish (Sigma-Aldrich, Taufkirchen), 0,1% v/v Tween-20). Primary antibodies were applied on the membrane overnight at 4°C at the following concentration: goat anti-DCX C18 1:1000 (Santa Cruz, California, USA), goat anti-EGFP 1:1000 (Rockland, Gilbertsville, PA, USA) and rabbit anti-actin 1:5000 (Sigma-Aldrich, Taufkirchen). Peroxidase-conjugated donkey anti-goat (Sigma-Aldrich) and peroxidase-conjugated donkey anti-rabbit antibodies were applied on membranes for 2 hours at room temperature. Following extensive washes, immuno-complexes were revealed using ECL-Plus Western Blotting Detection System (Amersham-Pharmacia, Freiburg) and photographic emulsion (Hyperfilm, Amersham-Pharmacia).
Cells were fixed for 30 minutes in phosphate-buffered 4% paraformaldehyde. Following washes, cells were blocked for 1 hour with gelatin-histology buffer (100 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% bovine serum albumin, 0.2% gelatin from cold water fish (Sigma) and 0.1% Triton X100). Antibody dilutions and subsequent washes with also performed in this gelatin-histology buffer. Incubation with the primary antibodies was performed overnight at 4°C at the following dilutions: mouse anti-A2B5 1:200 (Chemicon), mouse anti-βIII-tubulin 1:500 (Promega), goat anti-Doublecortin C18 1:250 (Santa Cruz Biotechnology), rabbit anti-GFAP 1:1000 (Dako), mouse anti-Map2ab 1:250 (Sigma), rabbit anti-nestin 1:200 (Chemicon), mouse anti-NeuN 1:500 (Chemicon), rabbit anti-NG2 1:200 (Chemicon). Fluorochrome-conjugated donkey secondary antibodies diluted 1:500 were applied for 2 hours at room temperature (Alexa Fluor, Molecular Probes). Nuclear staining was obtained using 0.25 μg/ul of DAPI before mounting in Prolong Gold antifade reagent (Molecular Probes)
bHLH, basic helix-loop-helix; DCX, doublecortin; EGFP, enhanced green fluorescent protein; NGF, nerve growth factor; Ngn2, neurogenin 2; NSE, neuron-specific enolase; RA, retinoic acid.
SCD Designed the study, perform reporter experiments and drafted the manuscript.
EQ Performed cell culture, expression and histological analysis.
KA Performed cell culture, expression and histological analysis.
CK Cloned the expression vectors.
SP Contributed to cloning, cell culture and expression analysis.
UB Contributed to the design of the study design and manuscript draft.
JW Contributed to the design of the study design and manuscript draft.
AL Contributed to the design of the study design and manuscript draft.
All authors read and approved the final manuscript.
We thank Dr. Francois Guillemot (National Institute for Medical Research, London, UK) for sharing a mouse Ngn2 expression vector. This work was supported by the German-Israeli Collaboration Program in Neurosciences from the German Federal Ministry for Education and Research BMBF grant 01GA0501 and BMBF grant 0312134, by the EU – FP6-project DiMI, LSHB-CT-2005-512146 and the Bavarian State Ministry of Sciences, research and the Arts (ForNeuroCell grant).
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text:
Leisure/Dorchester Horror Recommendations.
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Grand Sorcerer
Posts: 5,148
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Join Date: Nov 2008
Location: SF Bay Area, California, USA
Device: Clié; PRS-505; EZR Pocket Pro, PRS-600, Kobo Mini, Inkbook Prime
Dorchester has been not paying its authors, and several of them are
calling for a boycott
. They've also been putting up ebooks that they don't have the rights to--this is the real book piracy, the kind that copyright law was designed to counteract. Brian Keene says:
Since January of this year, unauthorized digital editions of my work have been sold via Kindle, Nook, iBooks, and Sony. These digital editions were not made available for sale until well after the rights had reverted back to me. Dorchester's response, in each case, has been to blame someone else and assure me that "they are looking into it" and that I would be "financially compensated" and that "it wouldn't happen again". Except that I haven't been financially compensated and it keeps happening again.
His post goes into details, with links to more posts, and a list of authors who are supporting the boycott.
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I rarely ever do. My old one was my display pic for the longest time i got sick of it and thought it was time for a change!
Most Helpful Guy
I had a profile picture once. Then someone (Who i think i pissed off at some point in my 12 months being here), Made a question saying ''Why is riggers so ugly?'', So i took it down, Haven't had one since (That was like in August or September i think).21|10|0
I hope you blocked him.
I don't know who it was, They were Anonymous. I ain't mad about it though, They weren't wrong.
Most Helpful Girl
Maybe 1-3 times a month? Something like that.
PS: u look beautiful.11|00|0
Thanks :)


text:
West Coast
Everything is changing at West Coast...to kick off the New Year we are bringing you all our exclusive album reviews and interviews and features online, freely available for everyone to see.
Once home to our quarterly music magazine covering West Coast Smooth, Fusion Grooves & Midnight Disco, you can still access our shop pages to purchase back issues, but they are running out, so anyone wanting to order should probably do so sooner rather than later
As always we feature music with that classic L.A. & California smooth groove sound from artists, bands and labels from around the world.
This music is for all the drifters and dreamers that remember when we had radio stations that championed the Doobie Brothers, Steely Dan, Hall & Oates, Patsy Gallant, Player & Andy Gibb and many more great AOR smooth gems from the 70's & 80's.
Plus you can buy all issues of West Coast Music Magazine past and present with one simple click.
Nick Mawson (Drifter, Dreamer & Editor)
Nick Mawson (Drifter, Dreamer & Editor)
Artist reviews of our Music Magazine
Bill LaBounty
"West Coast Magazine is something you shouldn't miss! Great articles and a great read"
Jerome Beuret (Louis Lake)
"A great magazine, beautifully made and of one of the last magazines of West Coast music in this world. I'm proud to be part of it with Louis Lake."
Tom Snow
Just got your latest issue. It's wonderful. Super cool graphics and a great layout. I love the presentation of my song list along with the terrific interviews. Best wishes for great success.
Shawn Lee (Young Gun Silver Fox)
"Great mag, thanks for the lovely feature. Silver Fox loves West Coast music magazine! Really cool, yeh baby!"
Ed Motta
"Great magazine. I loved the duos article. Everything about it, congratulations. All the best, Thank U. Ed"
Martijn Soetens (Music Director Soul & Jazz radio)
"A must have for anyone who loves west coast music. The magazine is a great way to discover songs you've never heard before."
Barney Hurley
(Samuel Purdey/Powder Blue Tux)
A magazine covering this genre of music should have been out decades ago. The most Informative and Insightful music magazine I've ever read. Buy a copy, now!
Marcus Liesenfeld (DJ Supermarkt) Too Slow Too Disco
"Thanks from DJ Supermarkt and Too slow to Disco for the fantastic new West Coast magazine."


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Yo, so I just went on my profile, and I got a percentage (17%), and I assumed that must be my opinions to MHO-ratio. But it says "17 out of 58", when i've answered over a hundred. What does the "58" represent then, if not total questions answered?
A question about user percentages?
Have an opinion?
What Guys Said 2
I believe the number is based on questions that have MHO selected. What I mean is that not everyone selects MHO for their questions so that wouldn't count on your percentage as a non MHO post. If you get my drift. Just say you make 100 posts, only 50 of the questions you answered have been given MHOs and only 8 of those 50 were your opinions. Something like that?44|00|0
Our FAQ is full of information like this. https://www.girlsaskguys.com/faq#178
Not every Question you share on has a Most Helpful Opinion. 58 is the number of Questions that have MHOs.00|00|0
Ah, I should have checked it more thoroughly, thank you.
What Girls Said 3
It means you've only made 58 comments. It doesn't count commenting on other people's comments though.22|00|0
So wait, you're saying it counts comments as "opinions"? It says on my profile though, 'Total Opinion (s) 167', And honestly, there's no waaaaay i've posted more than, say, 30 comments in total.
- Show AllShow Less
MHO % (found on your profile page) reflects how many times you've been awarded Most Helpful on Questions with a MHO.
For example, if you shared 100 Opinions on 100 Questions who have MHOs and you were selected for 50 of those Opinions, your MHO % would be 50% (50 out of 100).
yeah I think comments are just sort of aggregated at the bottom of your profile in a feed kinda so it doesn't really get counted? They always had a problem with comments actually, due to the web host I think. They've never been able to impliment a notification system for comments, which I think they were trying to do a while back, like you have with Facebook
I'd likta know that too..:/11|00|0
I think the MO is kind of an odd number. Not sure if I buy it. Also most folks come here to get the answers they are looking for I think.00|00|0
Learn more
Select as Most Helpful Opinion?
You cannot undo this action. The opinion owner is going to be notified and earn 7 XPER points.


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The Miami Dolphins have added three veteran defensive players in the free agent period: Mario Williams, Byron Maxwell, and Kiko Alonso. This article discusses whether this is the beginning of a turnaround for the Miami Dolphins, or whether the Williams free agent signing and the Maxwell/Alonso trade are another example of the Dolphins spinning their wheels.
It's no secret: Howie Roseman has been unloading Chip Kelly's players from the Philadelphia Eagles. That talent unload allowed the Miami Dolphins to collect a couple of defenders, both of whom come with question marks. The signing of Mario Williams to a player-friendly contract should give the Dolphins Defense a better pass rush — something they definitely needed after Olivier Vernon signed a blockbuster deal with the New York Giants.
Let's start with the more controversial or question-filled move of the two: the trade for Maxwell and Alonso. This move got a lot of criticism from NFL observers, but it has the potential to start changing the Dolphins' locker room culture.
Byron Maxwell and Kiko Alonso Traded to Dolphins
The Philadelphia Eagles have traded Byron Maxwell and Kiko Alonso to the Miami Dolphins for the right to flip picks in the 1st round of the 2016 NFL Draft. Ultimately, the Eagles move up into the Top 10, while the Dolphins move down to the 13th overall pick, while adding two veteran defensive starters.
The early buzz is the Eagles pulled off a major coup, simply getting rid of the Maxwell and Alonso contracts. People believed Byron Maxwell to be overrated and inconsistent, while Kiko Alonso is unreliable, due to injuries and other off-field concerns. Both could be valuable players in the right situation, but it's amazing a team would take their salaries and drawbacks in exchange for any alteration to their 1st round selection process.
Trade Nearly Didn't Happen
The trade which would send cornerback Byron Maxwell and linebacker Kiko Alonso to the Miami Dolphins appears to be active again. If completed, the Dolphins would acquire Maxwell and Alonso from the Philadelphia Eagles. In exchange, the Eagles would move from the #13 pick to the 8th pick in the 1st round, while the Dolphins would correspondingly move down 5 spots in the 1st round.
Due to an injury to Eagles cornerback Byron Maxwell, the trade between the Miami Dolphins and Philadelphia Eagles was rumored to be a dead letter. The Dolphins were said to be "gathering information" on the Maxwell injury.
Shedding Byron Maxwell's Contract
The Eagles appear to be out from under the weight of the six-year, $63 million contract Byron Maxwell contract. An injury to the former Seattle Seahawks cornerback might keep it from happening. NFL pundits are likely to think the injury is saving the Dolphins in this case.
Yesterday, the trade was said to be off, but Maxwell's agent, Alvin Keels, said this morning it was once again going to happen. Whatever the concerns the Fins had, it no longer is an issue.
Former Super Bowl Defensive Back
Byron Maxwell was the cornerback on the other side of the field from Richard Marshall during the Seahawks' two Super Bowl runs. Maxwell had good numbers in that time, because he was on a unit with 3 Pro Bowlers (Marshall, Kam Chancellor, and Earl Thomas). Because he was opposite of Marshall, he often played the opposing team's 2nd receiver and often received double-team help (from a Pro Bowl safety).
Under the circumstances, Maxwell flourished. But as one NFL media member said recently, Peter King said during Super Bowl LIX, "Someone's going to overpay in free agency for Byron Maxwell."
Sending away 2 Disastrous Chip Kelly Decisions
That team ended up being the Philadelphia Eagles, led by Chip Kelly at the time. The huge contract became an albatross last year, as Maxwell racked up 2 interceptions and 64 tackles in an inconsistent season.
Kiko Alonso had a second injury-prone, unsuccessful season in 2015. The 3-year veteran has a stellar rookie season in 2013, leading the Buffalo Bills in tackles and piling up huge IDP stats in fantasy. Then he tore an ACL in 2014, got traded straight-up for LeSean McCoy in 2015, and amassed only 44 tackles to justify that trade.
Mario Williams Free Agent Signing
Mario Williams signed a free agent contract with the Miami Dolphins for 2 years and $17 million. The deal was described by the Miami Herald as a "team-friendly contract", because it allows the Dolphins to escape from its terms for $2 million in dead money, if they walk away from the deal in 2017.
This could very likely be a one-year deal, because the base salary in 2017 is $8 million+ with incentives for $20 million. While Mario Williams would have to be close to the Defensive Player of the Year to reach those numbers, he's going to need to become a vital cog in the defense to justify the $8 million price tag next year.
Still, Mario Williams is still only 31 in a league when pass rushers can still get to the quarterback into their mid-thirties. Mario Williams has shown he can be a versatile defensive player, as he's played both 4-3 defensive end and 3-4 outside linebacker in the last several seasons.
Defensive end in the Dolphins' 4-3 Defense is his natural position. The team has to figure out what it's going to do with Cameron Wake and former 1st round pick Dion Sims. After letting Olivier Vernon go in free agency — for a massive contract to the New York Giants — this is a good plug-and-play free agent deal.
Isa Abdul-Quddus Signs with the Miami Dolphins
In late-breaking news, 5-year veteran safety Isa Abdul-Quddus has signed a $12.75 million, three-year contract with the Dolphins. Isa Abdul-Quddus was a starter for the Detroit Lions, before his signing.
Outlook for the Philadelphia Eagles:
This is a recurring theme for the Philadelphia Eagles, but this represents a chance to sculpt their roster by simply purging it off Chip Kelly additions. One gets the idea there is a psychological component to Howie Roseman trading all the players Chip Kelly added last year. Howie Roseman was the Eagles GM who hired Chip Kelly, but he lost a power struggle in the 2015 offseason and the former Eagles coach became the GM. Chip Kelly even had Howie Roseman's office moved from the Eagles' training facility to some accounting center.
Now, Chip Kelly left town under a cloud and Jeffrey Lurie repented; Howie Roseman is now the Eagles' GM once again. He wasted no time in getting rid of the 2015 additions. If the idea is to get rid of big contracts and ill-fitting pieces, then these moves should be lauded. The Byron Maxwell and Demarco Murray contracts were massive burdens on the Eagles' salary cap.
At the same time, one has to realize that three starters were traded away and no player additions were made. The Eagles still have the same number of draft picks them had before the trades, though they did move up a bit in a couple of rounds.
Prediction for the Miami Dolphins:
The Dolphins have added 3 veteran defensive players in the first two days of free agency. These moves have bolstered all three units on the team. If Mario Williams and Kiko Alonso can stay healthy, they should be able to help the Dolphins improve their defense.
Now that Mario Williams is past 30, playing at his natural defensive end position is key to his health. It was always a little unstable that a 6'7″ lineman might be dropping back in pass coverage sometimes. It's my feeling that playing outside linebacker has contributed to his injuries in his time with the Buffalo Bills. When he was healthy, the Bills were a very good defense the past couple of years.
Williams' former Bills teammate sits at a crucial point in his career. Can Kiko Alonso prove that the 21 missed games in 2014 and 2015 were a fluke? Can he show that the breakout, stellar rookie season wasn't a fluke? Or is he going to go down the road of Sean Lee: great for the four games a year he's healthy? It should help having beefy defensive tackles like Ndamukong Suh in front of him.
Any one of these moves is not a difference-maker. That being said, if the Dolphins can keep adding solid veteran players and those players stay healthy, it should help create a more veteran and responsible team. I remember the year Bill Belichick first took the New England Patriots to the Super Bowl. In the offseason, the Patriots signed something like 17 veteran free agents.
Most of those players were not marquee names. They were solid veterans like Bryan Cox, Mike Vrabel, David Patten, Larry Izzo, Roman Phifer, Terrell Buckley, Marc Edwards, and Antowain Smith. While no one player turned the Patriots around, the whole was greater than the sum of the parts. A new culture was established in New England. Hopefully, the Dolphins can do the same with these trades and signings.


text:
- Description
- View Full Specifications
Best Price In The Market!
- Year:
- 1999
- Length:
- 36'
- Engine/Fuel Type:
- Twin / diesel
- Located In:
- Orange Park, FL
- Hull Material:
- Fiberglass
- 74958-2948557
- Last Listed Price:
- Sold
There are boats that help define a particular manufacturer and for Luhrs, the 36 Convertible, was just such a boat. The boat has great looks, innovative features, good performance on the water and is ruggedly constructed.
The one feature that sets this boat apart is the molded-in stairs to the flybridge. This was a hugely popular feature that makes access to the bridge easier and safer. But what made this boat a truly family friendly sport fishing machine was the totality of the design. The two stateroom interior was well laid out with an island berth in the bow and guest stateroom with upper and lower berths to starboard. You could also sleep another in the pull-out hideaway berth in the salon settee. There is a head with stall shower and a nice size galley with separate refrigerator and freezer. The cockpit features a transom door, livewell, bait prep area and plenty of space to fish.
The Apex is an original owner boat with low hours on a pair of Cat 3126 / 420 hp diesel engines. The boat has had good care and shows well.
Full specs and pictures are on-line.
Direct Line 904-631-6658
Please contact Jon Hamilton at 904-387-5432
Additional Specs, Equipment and Information:
Boat Name
The Apex
Hull Shape: Modified Vee
LOA: 38 ft 11 in
Beam: 13 ft 10 in
Maximum Draft: 3 ft 5 in
Bridge Clearance: 15 ft 8 in
Headroom: 6 ft 4 in
Deadrise: 15 ° at Transom
Dry Weight: 22000 lbs
Total Power: 840 HP
Engine 1:
Engine Brand: Caterpillar
Year Built: 1999
Engine Model: 3126
Engine Type: Inboard
Engine/Fuel Type: Diesel
Location: Starboard
Engine Hours: 1350
Drive Type: Direct Drive
Engine Power: 420 HP
Engine 2:
Engine Brand: Caterpillar
Year Built: 1999
Engine Model: 3126
Engine Type: Inboard
Engine/Fuel Type: Diesel
Location: Port
Engine Hours: 1350
Drive Type: Direct Drive
Engine Power: 420 HP
Fresh Water Tanks: (94 Gallons)
Fuel Tanks: (400 Gallons)
Model History
The Luhrs 36 Convertible was produced in St. Augustine, Florida from 1998 thru 2007 and has proven to be one the manufactures most popular models. She was the first of a new generation of Convertible models for Luhrs incorporating stairs leading to the flybridge thus removing the vertical ladder arrangement common to sport-fish models of the day. Not only is this arrangement much more convenient, but it is also much safer for children, and yes, even pets! After a remarkable 10 year production run, the 36' Convertible as replaced in in 2008 by an all-new 35' Convertible.
Luhrs used tried and true construction techniques in the building of the 36 Convertible. The boats are made with E-glass fiberglass woven roving and polyester resin. The hull is laid up with a solid fiberglass bottom and the hull sides are cored with balsa above the waterline. The superstructure is solid glass with balsa cored decks. The stringer system is made of marine grade plywood and is completely encapsulated in fiberglass. The Luhrs product is designed for offshore use and is heavily built. The hull design features a double chine configuration and single lifting strakes port and starboard. The running gear is a conventional straight shaft design with bronze struts and rudders, Aquamet stainless steel shafts and Nibral (4) blade propellers. (There are no recesses or prop tunnels for the propellers)
Vessel Walk Thru
Boarding though a large transom door, the cockpit is large and provides pleanty of space for serious sportfishing. There is a molded in fishbox in the transom and hawse pipes port and starboard for leading docklines under the gunnels. A large built-in livewell is forward that also serves as a convenient bench seat for crew. Storage and coolers are built into the molded fiberglass steps.
The fiberglass steps lead to a comfortable flybridge with captain and companion pedestal chairs. There is a large console with flush mounted electronics and engine controls. Forward is a wide bench seat and plenty of storage forward in the flybridge brow.
Entering the salon through a sliding door the space is bright and airy with huge u-shaped lounge to port, and long galley to starboard. Entertainment center, galley built-ins, and plenty of storage complete the salon.
Below three steps is the guest stateroom to starboard, with settee that converts to over and under bunks.
Across the passageway is the full head with large shower stall and electric head and vanity.
At the bow of the vessel is th master stateroom with queen island berth that has four drawers and plenty of storage under the berth. His and hers cedar lined hanging lockers are on port and starboard sides.
- Ultra-Leather Dinette with storage & Pull-Out Sleeper Sofa
- Cherry Wood Interior
- Padded Headliner
- High Low Varnished Table
- Digital Air Conditioning Controls
- Built-in Entertainment Center
- Hidden Electrical Distribution Panel
- Hidden Battery Switch Panel
- Cut Pile Carpeting
- Snap-In Carpet Cover
- Mini Blinds
- Padded Valance
- Recessed Overhead Lighting
- Recessed Speakers
- Engine Room Access Hatches
- Large Storage Cabinet
- 19" JVC Television
- Toshiba VCR
- Stereo System 10 Disc CD Changer
- (2) Stereo Speakers
- Rod Storage / Mechanical Locker Under Forward Seats
- 12v Lighting
- 120v Outlets
- CO Detector
- Aft Curtain
- Norcold Under Counter 120V/12V Refrigerator
- Norcold Under Counter 120V/12V Freezer
- Built in GE Microwave
- Built in Black & Decker Coffee Maker
- Princess 2-Burner Electric Stove
- Formica Countertops w/Corian Fiddles
- Round Stainless Steel Sink
- Cherry Wood Cabinets
- Pull Out Pantry
- (4) Overhead Storage Cabinets
- (3) Drawers
- Courtesy Lighting
- Water & Holding Tank Gauge
- 12v Lighting
- 120v Outlet
Master Stateroom
- Queen Island Berth with Inner Spring Mattress
- Flip Up Under Berth Storage and 3 Large Drawers
- Custom Comforter w/ Throw Pillows
- Air Conditioning
- Cherry Wood Interior
- Cut Pile Carpeting
- Snap-In Carpet Cover
- Built-in Night Stands Port & Starboard w/ Storage Under
- Individual Reading Lamps
- 13" TV & Stereo w/ Speakers
- His and Hers Cedar Lined Hanging Lockers, Lighted
- Padded Headliner
- Padded Hull Liner
- Recessed Overhead Lighting
- Overhead Storage Cabinet Starboard Side
- Overhead Hatch w/ Screen
- Private Entrance to Head
- CO Detector
- 12v Lighting
- 120v Outlets
- Vac-U-Flush Head, Macerator and Holding Tank with Overboard Discharge
- Overhead Hatch w/ Screen
- Air Conditoning Vent
- Recessed Lighting
- Corian Counter Top Vanity
- Large Stall Shower
- Bi Fold Shower Door
- Molded Seat In Shower
- Shower Sump Pump
- Private Door to Owner's Stateroom
- Fiberglass Liner
- 12v Lighting
- 120v Outlets
- Overhead Storage Locker
- Medicine Cabinet w/Mirrored Front
Guest Stateroom
- Settee converts to Upper & Lower Berths
- Rod Locker Under Lower Berth
- Overhead Hatch w/ Screen
- Large Hanging Locker w/ Shelf Over
- Individual Reading Lights
- Cut Pile Carpet
- Snap-In Carpet Covering
- Air Conditioning Vent
- 12v Lighting
- 120v Outlet
- CO Detector
- BridgeWalk Molded Stairway To Bridge
- Large Console w/ Flush Mounted Electronics and Engine Controls
- Half tower w/ Full Enclosure
- Storage in Bridge Coaming Forward
- Captain & Companion Pedestal Chairs w/ Footrests
- Large Settee w/ Storage Under
- Rocket Launcher For (6) Rods
- Diamond Pattern Molded Non-Skid
- Aluminum Handrails
- Cup Holders
- 120v Outlet
- New Bridge Upholstery
- (2) Stereo Speakers
- Sea Star Hydraulic Steering
- Cast Aluminum Sterning Wheel
- Teleflex Stainless Steel Shifters & Throttles
- Windlass Control
- Bennett Trim Tab Controls
- Glendinning Engine Sychronizer
- Sea Fire Engine Shut Down Overide
- VDO Engine Instruments: Tach, Volts, Temp, Oil Press, Fuel
- Keyless Ignition System
- Dual Pedestal Seats w/Footrests
- Raytheon RL70 Pathfinder Radar / GPS Chartplotter
- Raytheon 48 Mile Radar Antenna
- Raytheon L750 Fishfinder
- Raytheon RayPilot 650 Autopilot
- Raytheon Ray 220 VHF/Hailer
- Richie Powerdamp Wet Compass
- Richie Electronic Compass
- Electronics Cover
- Jensen AM/FM/CD System On Flybridge
On Deck
- Lee Double Spreader Outriggers (currently off boat)
- Molded Livewell/Bait Prep Area
- Molded Fish Box in Transom
- (6) Flush Mounted Rod Holders
- Self-Bailing Cockpit w/ Large Scuppers
- One Piece Welded Bow Rail
- (2) Bow, (2) Spring & (2) Stern Mooring Cleats
- Rub Rail w/ Stainless Steel Insert
- Cockpit Steps Port & Starboard w/ Storage
- Anchor Windlass w/ Foot Switches and Helm Switch
- Bow Pulpit w/ Anchor Storage
- Anchor Safety Latch
- Anchor Locker
- Lighting Under Gunwale
- Aluminum Plate in Deck for Fighting Chair
- Fresh Water Washdown
- Raw Water Washdown
- Lazerette Hatch w/ Lift Out Storage box
- Cockpit Flood Lights
- Hailer Horn
- Hawser Pipes For Transom Cleats
- Fresh & Raw Water Washdown
- 240v / 50 amp Shore Power Inlet w/50' Cable
- Under Gunwale Rod Storage
- Armstrong Boarding Ladder
- Last Bottom Job June 2015 with Epoxy Barrier Coat
- Twin Caterpillar 3126 Diesel Engines Rates at 420hp w/1,350 Hours
- 8 KW Kohler Generator w/990 Hours
- Aquamet Stainless Steel Prop Shafts
- Bronze Thru-Hulls Below Waterline w/ Seacocks
- Freshwater Cooling System On Main Engines & Generator
- Manganese Bronze Struts
- Manganese Bronze Rudders
- Bronze Thru Hulls & Ball Valves
- Compress Style Shaft Logs
- Stainless Steel Adjustable Tie-Bar
- (2) Marine Air Reverse Cycle A/C & Heat Units, 7,000 BTU & 16,000 BTU
- Oil Exchange System
- Vacuflush Head System
- Hydraulic Steering
- Racor Diesel Fuel Filters/Separators
- Aluminum Fuel Tank, Single Fill
- Engine Mufflers
- 94 Gallon Plastic Water Tank
- 30 Gallon Plastic Waste Tank Monitor
- Dockside Water Connection
- Bennett Hydraulic Recessed Trim Tabs
- Automatic Fire Extinguishing System
- Engine Alarm System
- Engine Room Ladder
- 3 Automatic 2000 GPH Bilge Pumps
- Water Heater
- 12v Engine Room Lighting
Electrical Systems
- 8 KW Kohler Generator w/ Separate Starting Battery
- 120V/12V Ships Electrical Panel
- Automatic 12V Battery Charger
- (2)8-D Engine Start Batteries
- (1) Group 27 Generator Battery
- Ground Fault Current Protection
- 50 Amp Shore Power Connection
- 50 Amp Shore Power Cord
- Battery Switch Panel Above Deck
- Cable TV Connection & Wiring
- Telephone Connection & Wiring
- Color Coded Wiring Harness
- Vessel Bonding System
- Galvanic Isolator
- GFCI Outlets
- 12v Lighting
Manufacturer Provided Description
Luhrs has included everything but the tackle to make the 36 Convertible one of the most complete sportfisherman on the market. The 36 includes bait prep and rigging stations, washdowns, rod holders, rocket launchers, and a complete package of Coast Guard-approved safety equipment. With every Luhrs, three generations of experience combine with modern engineering and production boat-building techniques to provide one of the most affordable, full-featured fishing boats in the industry. From the tower to the transom door, every piece of equipment and its placement is engineered to provide the best advantage on the water.
The Company offers the details of this vessel in good faith but cannot guarantee or warrant the accuracy of this information nor warrant the condition of the vessel. A buyer should instruct his agents, or his surveyors, to investigate such details as the buyer desires validated. This vessel is offered subject to prior sale, price change, or withdrawal without notice.
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Question: Niner Bikes as discussed in the chapter opener uses a
Niner Bikes, as discussed in the chapter opener, uses a costing system with standard costs for direct materials, direct labor, and overhead costs. Two comments frequently are mentioned in relation to standard costing and variance analysis: "Variances are not explanations" and "Management's goal is not to minimize variances."
Write a short memo to Chris Sugai, Niner Bikes' president, (no more than one page) interpreting these two comments in the context of his business.
View Solution:
Write a short memo to Chris Sugai, Niner Bikes' president, (no more than one page) interpreting these two comments in the context of his business.
View Solution:
Answer to relevant QuestionsAccess the annual report of Samsung (at samsung.com) for the year ended December 31, 2013. The usefulness of its budgets, variances, and related analyses depends on the accuracy of management's estimates of future sales ...Jansen Company reports the following for its ski department for the year 2015. All of its costs are direct, except as noted. Sales . . . . . . . . . . . . . . . . . . . . .$605,000 Cost of goods sold. . . . . . . . . . ...Apple and Google compete in several product categories. Sales, income, and asset information is provided for fiscal year 2013 for each company below. Required 1. Compute profit margin for each company. 2. Compute investment ...Park Co. is considering an investment that requires immediate payment of $27,000 and provides expected cash inflows of $9,000 annually for four years. If Park Co. requires a 10% return on its investments, what is the net ...A company has already incurred $5,000 of costs in producing 6,000 units of Product XY. Product XY can be sold as is for $15 per unit. Instead, the company could incur further processing costs of $8 per unit and sell the ...
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Private House On Golf Course With Mountian Views and Outdoor Kitchen W/firepit
Please note that if you or your group are looking for multiple houses within Palm Springs, that I have three similar homes in central Palm Springs of of Sunrise that are very similar to this listing and all houses are within the same neighborhood with two of them being side by side and the third house just around the corner from the two houses that are adjacent to each other. In addition, I then have this listing on the Tahquitz legends golf course as well as another listing that is located on the 4th hole of the Tahqutiz Legends golf course. Thank you for taking the time to look at this listing as well as any of my other houses listings that can be viewed on VRBO by clicking on the link below that states view owners other properties.
Also note that the gas fee is not charged during the summer months of June/July/August and begins on September 15. The summer months however do incur a daily utility charge of $50, so any inquires of dates during that time frame will show the gas fee/utility fee in the quote as well due to the electrical tier price increase that Southern California Edison charges. Should you elect or want to reserve/book online and do so during the summer months, the gas fee/utility fee will be charged. Further, also note that the gas fee unfortunately can not be calculated as a daily rate during the winter months and will only show as a $50 daily charge, so any vacation request during the winter months will have the gas fee charged accordingly up to the maximum weekly fee. So please again note that when figuring out your rental rate as the rate will more than likely be a bit higher to account for the added gas usage charges up to the maximum amount on a weekly or daily basis. Should you elect or want to reserve/book online the correct gas fee will then be calculated by me based on days and or weeks the property is rented and charged accordingly. Again, I will edit your rental request as necessary for the gas fees and then send you an updated quote to rent the house for the correct amount.
This is my fourth listing in Palm Springs and is another beautiful get-away home on a large 13,000 plus sq ft lot. This completely remodeled hi-tech home is gorgeous, well-appointed and captures the vibe and sophistication of the Southern California lifestyle or the mid century beauty of Palm Springs with the newly remodeled polished concrete floors throughout the house. This home is located on a golf course with secluded privacy with beautiful mountain views and desert landscaping throughout. Located in south palm springs, this modern 3 bedroom, 3 bath, 1702 sq ft single level house is a quiet retreat ideal for rest, relaxation or a group retreat with the idea of resting/relaxing at the house during your stay. With the spacious open floor plan inside the home you will find ample seating for everyone or go outside and relax by the pool in one of the numerous chaise lounges or deep seating collections under one of the two lanai's or relax in the attached spa while you gaze at the serenity of the property and sunsets at night. The backyard features a newly constructed outside entertainment area, featuring a large beautiful rocked fireplace under the large lanai, a fire pit near the golf course with amazing sunsets at night and a custom outdoor kitchen with built in 40" profession Lion BBQ, under mount refrigerator and sink, along with the numerous counter high chairs to sit around on the counter area and watch television or just gaze out at the sunset and or property. Additionally the outdoor kitchen features a 55" flat screen tv under the lanai, that is hung above the fireplace and then another 47" flat screen tv under the attached patio cover near the master bedroom slider for further outdoor entertainment/viewing of your favorite sport teams, or simply sitting and enjoying music and or television while outside. All tv's are set-up with direct tv, so you will have many viewing options to choose from. The main patio cover with the bbq and sink also features an outdoor heater for the cooler months which will enable you to enjoy your time outside late into the evening, along with the heat from the fireplace.
This 3 bedroom home is beautifully furnished with a large open living area that flows into the kitchen with a large flat screen 55" TV over the beautiful rock dual fireplace and then a 40" TV adjacent to the kitchen island. The 55" TV is a smart television allowing you access to stream some of your favorite movies from your streaming accounts or you can utilize the Blue Ray DVD player that is hidden behind the TV to watch some of your favorite movies. The user-friendly kitchen includes all modern appliances and is well stocked with glass, dining, and cookware. There is a total of three refrigerators with the large refrigerator, one beverage refrigerator on the kitchen island along with one wine chiller/refrigerator also located in the kitchen island. Two sliding glass doors lead to the large private backyard and pool area, from both the kitchen area and then the large adjacent living room. The outdoor dining area is also covered under the main lanai with two ceiling fans and light along with the 40" gas Lion professional BBQ that was previously mentioned.
The pool has been recently remodeled to include color led lights with four deck jets spraying into the pool along with a large tanning shelf near the jacuzzi, which makes the backyard perfect for all types of activities or just lounging for all guest at the house. Complete outdoor privacy provides the opportunity for swimming and sunning for those who choose. Inside the house, air-conditioning and ceiling fans allow for complete interior comfort, day or night. High speed wireless internet is included. For our guest's added convenience, there is a washer/dryer located in the garage for use during your stay. The home features a two-car garage as well for those wishing to park their cars inside.
The house has two master suites. The farther or back master suite has a sliding glass door that leads to the backyard has a Cal king bed, along with its own bathroom with dual vanity bath sinks as well as a dual head walk in shower. The sliding glass door leads out from this master bedroom to a covered lanai with seating area to enjoy the pool and spa or to gaze out at the golf course or surrounding property. The second master suite has a standard king bed along with its own private bathroom featuring one vanity sink along with a walk in shower. This bedroom also has a 48" flat screen tv to enjoy as well. The middle or hall bedroom has a queen bed with comfortable furnishings. All three bedrooms are equipped with flat screen televisions with direct tv satellite service. Total house can sleep six people very comfortably and up to eight with the queen floatation mattress or the couch in the living room. Perfect house for friends, three-four couples or a large family.
The PS Links house is located on a quiet residential street in the desirable Tahquitz Creek Golf Resort, which has been named by Golf Digest "One of the Best Places to Play" for golf resorts. The Legend Course which the PS Links house is located on was designed by William Francis Bell, also known for designing Torrey Pines, La Jolla Country Club and Bermuda Dunes Country Club.
PS Links is close to the downtown shopping strip, the aerial tramway, world gym, the riviera club, three shopping malls, excellent restaurants, medical facilities, spa locations, worship centers, award-winning golf courses and several casinos. The residence is non-smoking, although smoking is permitted, outside. All linens are provided, along with bath towels and includes swim towels as well. Families with infants and or children of all ages are welcome, however, the pool area is unfenced, so we discourage unattended toddlers and younger children swimming or to be unattended at anytime while in the backyard near or around the pool. Well behaved dogs are also welcome.
We cherish our escapes to our own desert getaway, as we always have valuable rest and memorable fun. Please come experience this house as we're sure you'll enjoy it as much as we do.
Palm Springs Transient Occupancy Registration Permit #6159
- Guests Provide Their Own Meals
- Air Conditioning
- Heating
- Linens Provided
- Dishwasher
- Refrigerator
- Stove
- Oven
- Microwave
- Washing Machine
- Clothes Dryer
- Fireplace
- Television
- Satellite / Cable
- Parking
- Garage
- Not Suitable For Children
- Pets Considered
- Romantic
- Family
- Away From It All
- Dining Area
- Internet
- Towels Provided
- Child's Highchair
- Non Smoking Only
- Heated Pool
- Private Pool
- DVD Player
- Coffee Maker
- Toaster
- Hot Tub
- Concierge
- Outdoor Grill
- Iron & Board
- Hair Dryer
- Dishes & Utensils
- Living Room
- Kitchen
- Deck / Patio
- Lanai / Gazebo
- Golf


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"First Class Service"
About: Southmead Hospital Southmead Hospital Bristol BS10 5NB
Posted by Bri4070
I had an appointment at the Urology Department in Brunel Building. The self check in screens quickly directed me to the correct department, where I was seen by medical staff, at the time shown on my appointment. Staff were very courteous and friendly. Couldn't wish for better treatment, personal and medical.
Do you have a similar story to tell? Tell your story & make a difference ››


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NCHMCT JEE Result 2015
National Council for Hotel Management and Catering Technology is releasing the NCHMCT JEE Result 2015 for those candidates who are participated in the entrance exam for admission in the various courses. We have provided some important information regarding the same such as easy steps to check entrance exam merit, important dates etc.
4 Easy Steps to Download Merit List
- Candidates firstly visit to the official website i.e. www.nchm.nic.in
- Then select "NCHMJEE Result and counseling". Now, follow "JEE Result"
- Enter the Roll No. and DOB. Then press Submit button.
- Score card will display on your screen. Download the rank card and take a printout for future use.
Conducting Body:
National Council for Hotel Management and Catering Technology conducts the national-level Joint Entrance Examination to give admission to the merit students in its hospitality courses such as B.Sc Hospitality and Hotel Administration etc.
Type of Exam:
National Level Exam
Exam Category:
Under Graduate Level
National Council for Hotel Management and Catering Technology (NCHMCT) is releasing merit list for those candidates who have appeared in the examination. Students who are curiously waiting for the score card they may check and download the Entrance Exam Merit List by this page only.
Every year huge no. of students participates for NCHMCT JEE examination to get admission in the top hospitality colleges in India. National Council for Hotel Management and Catering Technology, Joint Entrance Exam (NCHMCT JEE) merit list is expected to be declared in the month of May 2015.
Important Dates of 2015:
- Application form available- December (Tentative)
- Last date of submission- March (Tentative)
- Exam Date- April (Tentative)
- Result date- May (Tentative)
You May Also Check
|Best Hotel Management Colleges In Mumbai
|Hotel Management Colleges in Delhi NCR
Note: You may get score card through the Official Website.


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We just came back from a long weekend at the Sea Rose Suites, which I found based on the Tripadvisor reviews. I can confirm that the reviews steered us in the right direction! The location of the inn is perfect, just about halfway between the downtown area and Perkins Cove, and across the street from the entrance to Marginal Way, which is a beautiful walkway along the ocean leading right to Perkins Cove. Everything was clean and nice, without feeling generic or sterile. We did not use the pool or hot tub but saw some familes using the indoor pool (outdoor pool was closed after Columbus Day, I believe).
Our room was perfect-- a 1 bedroom in the main building. The bedroom was a nice size, good sized, clean bathroom with plenty of hot water and nice toiletries (Philosophy brand). The living area included a kitchenette (fridge, microwave, sink, glasses, plates, utensils, everything except an oven), a table and chairs, and a sofa and TV. It was very spacious for a couple.
The only thing I could possible consider downside is that we could hear noise from the street, cars passing, people walking by if they were particularly loud. It wasn't bad, and we opted to keep the window open most of the time which shows how little it bothered us, but if you are a very very light sleeper, you might want a room on the back of the inn away from the street. Oh, and wifi is free, but it did make us keep reentering the password. Minor quibble.
As others have said, no food service, but I would not have eaten here anyway-- too many other places to try a short walk away!
All in all, I would highly recommend this inn to any of my friends and would stay here again if we make it back to Ogunquit.
- Official Description (provided by the hotel):
- The Sea Rose Suites is a comfortable, all suite hotel centrally located on Shore Road in Ogunquit, Maine. We are situated between Ogunquit Village with its small shops, restaurants and beautiful beach and quaint, charming, Perkins Cove. Our luxurious suites, many of which are recently renovated, all include a kitchenette and our hotel boasts indoor and outdoor pools, guest laundry, a fitness room, and ample guest parking. ... more less
- Reservation Options:
- TripAdvisor is proud to partner with Booking.com, Hotels.com, Expedia, Priceline, Travelocity, Orbitz, TripOnline SA, Hotwire and Agoda so you can book your Sea Rose Suites reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Sea Rose Hotel Ogunquit
- Sea Rose Suites Ogunquit, Maine


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Coming Through the Rye, set in 1969, is a touching coming of age story of sensitive, 16 yr. old Jamie Schwartz, who is not the most popular kid at his all boys' boarding school. Disconnected from students and teachers, he believes he is destined to play Holden Caulfield, the main character of The Catcher in the Rye, and has adapted the book as a play.
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| This stub is making Kirby sad.
This article or section is a stub. You can help the Heroes Wiki by expanding it!
What are you waiting for? GO!
Reggie Mantle is the tritagonist of the Archie comic series. He is Archie's rival for the affections of Veronica Lodge and is also a member of Archie's rock band The Archies, playing bass guitar for the group.
He is usually considered the main villain most of the times, due to him being the absolute moral opposite of Archie Andrews.
- Master Strategist / Manipulator
- Expert Guitar Player
- Skilled Combatant
- Significant Wealth / Resources


text: At level 30, you can go to a riding trainer, for your race. The level 30 mounts go 60% faster, and the mount you get depends on your race. The riding trainer and mount vendor will be around the area where you start. In order to get other mounts for your faction, you will have to gain Exalted reputation with them.


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Rear Seat Spring Retainer; 50-52 Willys M38
This replacement rear seat frame spring retainer from Omix-ADA fits 50-52 Willys M38s. 2 are required per seat.
Pricing: $7.99
Direct OE replacement Jeep parts and accessories built to the original specifications by Omix-ADA. Limited five year Manufacturer's warranty.
- 12023.10
- 12023.10
- 804314039318
- Omix-ADA
- New
Shipping Information
- Item Requires Shipping
- 0.1 lbs.
- W1.0000" x H0.7500" x L5.5000"
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16 Super Delicious Dinner Recipes You Can Make During The Weekend
Dear friends for the free time in the weekend probably you will want to cook something delicious something that you can share with your friends and loved ones. If you want to grab some inspiration and ideas also a recipe than you are on the right place. We have collected 16 yummy dinner recipes that will surprise your guests. Lasagna, ham, stuffed pork, amazing chicken and much more just pick up what you like and cick on the source to find the recipe. Everything is super delicious. Enjoy!


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Combine brown sugar, mustard, and cornstarch. Slowly stir in vinegar. Add lemon peel, lemon juice, raisins, and butter or ham fat. Stir all ingredients in a medium pan over low heat. Makes 2 cups. This is very good spooned over ham.
By Robin from Washington, IA
Add your voice! Click below to comment. ThriftyFun is powered by your wisdom!


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The Early Show Entertainment Contributor and People magazine Senior Editor Jess Cagle will give a preview Friday of what looks to be the biggest and the best in the Weekend Marquee series.
Cagle says the films may be smaller, but the stars are just as big. This fall there seems to be something for everyone.
Intolerable Cruelty
George Clooney and Catherine Zeta-Jones team up for the Coen Brother's romantic comedy, "Intolerable Cruelty."
The Duplex
Ben Stiller and Drew Barrymore play New York homeowners who plot to get rid of their elderly tenant in "The Duplex."
Runaway Jury
An all-star cast including Gene Hackman, Dustin Hoffman and John Cusack bring John Grisham's best-selling novel "Runaway Jury" to the big screen.
Looney Tunes Back in Action
Animated all-stars Bugs Bunny, Daffy Duck and Wyle E. Coyote team up to help Brendan Fraser and Jenna Elfman save the world from Steve Martin in "Looney Tunes Back in Action."
Once Upon a Time in Mexico
Antonio Banderas and Johnny Depp star in "Once Upon a Time in Mexico," the final chapter in director Robert Rodriguez's "El Mariachi" trilogy.
The Fighting Temptations
In "The Fighting Temptations," Cuba Gooding plays a man who must create a successful gospel choir in order to collect an inheritance. He gets some help from pop star Beyoncé Knowles.
The Rundown
The Rock plays a bounty hunter tracking down a renegade, played by "American Pie's" Seann William Scott, in "The Rundown."
The School Of Rock
Jack Black plays a frustrated musician who poses as a substitute teacher and forms a fifth-grade rock band in "The School of Rock."
Former Saturday Night live star Will Ferrell plays a man raised by elves who sets out to find his roots in "Elf."
Scary Movie 3
Get ready for a third go-round of horror movie spoofs with "Scary Movie 3."
Texas Chainsaw Massacre
There's a remake of the horror classic "Texas Chainsaw Massacre" for some real scares.
Oscar-winner Halle Berry stars in the thriller "Gothika."
Out of Time
Denzel Washington plays a police chief who gets caught up in a web of his own lies in "Out of Time."
Under the Tuscan Sun
Diane Lane escapes her past and finds her future in "Under the Tuscan Sun."
Kill Bill
"Kill Bill: Volume One" reunites Uma Thurman with writer-director Quentin Tarantino. "Pulp Fiction" won him an Oscar and her a nomination.
Human Stain
This year's best actress winner, Nicole Kidman, teams up with Oscar-winner Anthony Hopkins and nominees Ed Harris and Gary Sinise for "The Human Stain."
Mystic River
Clint Eastwood stays behind the camera and directs "Mystic River." He leaves the performances to Sean Penn, Tim Robbins and Kevin Bacon.
Veronica Guerin
Cate Blanchett stars as a journalist investigating Irish drug lords in "Veronica Guerin."
Master and Commander
Russell Crowe follows up his 2001 Oscar with the high seas epic "Master and Commander."
Matrix Revolutions
And Nov. 5 brings the final chapter in "The Matrix" trilogy — " Matrix Revolutions."


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Burglauenen First rankings
Difficulty ranking world: 1484 (all)
Ranking Switzerland: 158 (all)
Difficulty score: 102.55 what?
Burglauenen First ratings
(rate it)
The Burglauenen First is situated in Bern. The Burglauenen First should be climbed with a mountainbike.
Starting from Burglauenen,
the Burglauenen First ascent is 31.85 km long. Over this distance, you climb 1274 heightmeters.
The average percentage thus is 4 %.
Look for other sides to climb the Burglauenen First.
Find climbs close to the Burglauenen First: within 10 km - within 20 km - within 50 km
Find all climbs and cols in Bern on a map.
No stories submitted yet. Did you climb this mountain?
Did you climb the Burglauenen First? Please send your cycling story, description or comments. You can also send us video's or send us pictures on the Burglauenen First.
© climbbybike™ All rights reserved 2005 -


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Yes! The time has come to announce the lucky winner of the 'Celebrate Sallu's Birthday with CNS' contest. As promised, the lucky person gets an awesome Bluetooth speaker as the reward for his/her hard work. We are thankful to all those who participated and would like to wish you the best for future contests.
Scroll down to know if you are the one who is going to take home the cool and trendy Bluetooth speaker home!
We request the winner to kindly inbox us their details on our facebook page.
Congratulations to the lucky one.
Copyright © ClapsnSlaps, Inc 2017.
SBB Venture Pvt. Ltd. All rights reserved.
The content on this site may not be reproduced, distributed, transmitted, cached or otherwise used, except with the prior written permission of clapsnslaps.com


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Featuring Model Prices and Specifications, plus Reviews,News and Videos.
To celebrate its 75th anniversary, Jaguar has announced Australian pricing and availability for the 2010 Jaguar XKR Special Editi...
Interested in a new car? Simply tell us the vehicle you are interested in and we can take care of the rest.
Get great offers, the latest news and reviews, photos, videos and more delivered straight to your inbox.


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The affluent town of Hoffman, New Jersey, reeled in disbelief when highly esteemed physician Duncan Avery stabbed his wife Marsha to death one spring evening. The two Avery sons turned their backs on their father, but his daughter Nina never stopped believing in his innocence. Now, 15 years later, Nina, a struggling actress in New York City, returns to Hoffman when her father is paroled and insists on re-settling there. Not only does Dr. Avery want to repair his relationships with his sons and meet his grandchildren, he claims he wants to find out who killed his wife. But neither Nina's brother Patrick, a successful investment banker with a wife and twin sons, nor Jimmy, a recovering drug addict who has adopted a new family, can bear to acknowledge their father's return.
Then, as suddenly as before, another act of violence overturns the Averys' lives. No longer certain if she can trust her family, her neighbors, or her old friends, Nina finds she can rely only on herself and a surprising new ally in her life as she searches for the truth behind crimes past and present. If she digs deep down into the secrets of her family's and her neighbors' lives she stands a chance of catching a killer before she becomes his next victim.
With psychologically perceptive characterizations, emotional realism, and a gripping story line, The Girl Next Door exposes the dark impulses and fierce passions that can rule seemingly ordinary people and showcases Patricia MacDonald at the height of her celebrated powers.
"MacDonald, the master of the small-town tragedy, delivers another sure winner." (Publishers Weekly)
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Kept me on the edge! Almost every chapter added another twist!
And the reader, Bernadette is AWESOME! Love her style!
Jimmy's return to his addiction!
This book won me over to Patricia! I just purchased another one!
- Skoutariotis


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Google+, the emerging social networking site is now busy in promoting some trust among the users in their platform. Google+ has recently added an entirely new attribute known as Badges to it. Badge is similar to a certification that is made on some specified user's profile and it is a dark gray colored checkmark engraved on the top of a circle of light gray. As the mouse is moved across it, it includes a phrase: Verified name. This feature is placed adjacent to the name of the user that is already verified by Google+.
As per an official of Google, this feature is to verify the real identity of the users. When the other users come across the profile of a public figure or a celebrity, they can see the verification badge adjacent to the profile name and hence helps the users in identifying the real profiles. Right now, this badge option is implemented only for celebrities, public figures, users added to innumerable users' circles, actors and politicians. Still the number of users who can follow a particular user is not announced by Google+.
The verification process or procedure used to verify the high profile personae and the celebrities is intact. Quite a number of users of that level are already authenticated by the site and few of them owning verified profiles are Dolly Parton and Facebook's Zuckerberg.
When it comes to social media or networking sites with genuine and real high profiled users, Google+ is surely a member in the list. Google+ along with Facebook and Twitter lets the users to interact completely and be updated with those they view as important in the current growing and living world wide digital community.
Google+ implemented a policy for real-name and this forces the users to create an identity for themselves and present them using their true name, disclosing their gender and more. This social media website has gone to the extent of deleting the accounts that proved to be false or doubtful too. This in-fact generated complaints from many users but the site has posed an amicable style of approach. Google+ offers the users with a way to repair and appeal the non-compliance.
Despite of this solution offered by the website, the users still press that they revise this policy. Some of them argue that certain users cannot reveal true identity or all the details and require anonymity like the political dissidents who voice out causes and opinions or the abused spouses coming out with the issues that happen to them. The anonymity will let them be protected and secured from the pseudonyms.
Google+ was launched in the month of June 2011 and has about 25 million members now. The member list goes on increasing with the users sending invites to others they know. The demand is growing largely since launch and the website has started to operate the beta testing version for the growing user list.


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Liquid ceramic thermal insulation coating
Found:9 products← Back to category"Liquid Insulation"
Liquid ceramic heat-insulating covering of TSM Ceramic, wholesale, Ukraine
In stock
Price:6.88 EUR
Liquid ceramic heat-insulating covering of TSM Ceramic. The unique heat-insulating material TSM Ceramic possesses the following properties: heat-in Read more
6 years on Allbiz
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This company replies on the enquiry during 24 hours
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Thermal insulation liquid Facade
In stock
Price:6.13 EUR
SCOPES 1.Thermal insulation of facades of buildings; 2.Elimination of "cold bridges"; 3.Thermal insulation of a roof of buildings and constructi Read more
Delivery fromBelarus
Energy saving KERAMOIZOL paint - a new type of heat insulation with use of nanotechnologies
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Price:2.67 EUR
The energy saving composition of "KERAMOIZOL" is absolutely new type of heat insulation with use of nanotechnologies. Minimizirovaniye or decrease Read more
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Warm g_dro_zolyats_yne pokrittya "Tezola
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Opis pokrittya keram_chny teplo_zolyats_yny "Tezolat" Teplo_zolyats_yne pokrittya "Tezolat" to the priznachena for warmly - g_dro - noise - _zolyats Read more
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Liquid hydro-thermal insulation, ultrotonky 0,6 mm
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Material consists of the tiny, hollow balls from ceramics which are in a suspension in the liquid composition consisting of rubber, polymers and inorg Read more
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The heatpreserving VD-AK-121T pain
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Our Forevermark Diamonds
▼ Our Forevermark Diamonds
We make it easy for our clients to understand the factors that make Devotion with Forevermark Diamond jewelry so rare and exceptional. When you work with a Devotion associate to select your diamond, you will learn about its shape, carat, clarity, color, sparkle, brilliance, fire, fluorescence, finish, symmetry, crystal structure, evaluation documents; and how each of these characteristics relates to its price.
On the following pages you will find some basic background on these diamond terms, and how Forevermark diamonds go beyond the standard 4Cs, to enhance your understanding.


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One new councilor elected in Hampden
Posted Nov. 03, 2010, at 2:42 a.m.
HAMPDEN, Maine — Kristen Hornbrook won a three-way race Tuesday for one open seat on the Hampden Town Council.
Hornbrook, with 1,424 votes, edged former Councilor Andrew Colford, 1,184 votes, and James Feverston, 415 votes.
The 40-year-old Hornbrook is self-employed and lives in Hampden with her husband and two children.