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Myles Garrett
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Magic Card of the Week: Spellskite
The New Phyrexia set gave life to possibly the most Lightning Bolt-frustrating card I've ever seen. For that, Spellskite has come forth as this week's Magic Card of the Week.
Dropping two colorless mana lets you pull Spellskite onto the battlefield, and with it's variable activated ability cost it could fit nicely into any deck.
If you like your life total where it is, keep this guy in a blue deck.
A natural 0/4, this Artifact Creature can cause any spell to target itself for the low cost of just one blue mana (or two life in a pinch).
He's tough enough to handle the light removal spells so he can selectively draw in spells multiple times. You can even use this creature to deny any buffs your opponent wants to put on his own creatures, simply redirect them to Spellskite.
A versatile tool for any control deck.

subset: commoncrawl


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|"Antonio Gallardo" <agalla...@agsoftware.dnsalias.com>
|Re: [RT] Woody and round trip of parameters.
|Thu, 31 Jul 2003 01:04:00 GMT
Marc Portier dijo: > > > Antonio Gallardo wrote: >> Marc Portier dijo: >> >>>I can't see a need for a field that should be 'calculated >>>automatically' AND be editable at the same time. >>>(I tried though) >> >> It sometimes happens, for example here in my country, in a restaurant >> invoice is a non written rule to pay 10% of the subtotal to people >> that serviced you. But if you dont want to pay this you can change the >> amount or not pay it at all. Then you need to create another invoice >> with the change. >> >> Also diplomatic people does not pay taxes. If you have an automatic >> formula to calculate the X % tax, when this people show you their ID, >> you need to put the tax amount to 0. >> >> I think is are two examples when a formula can be change by the user. >> > > I might be missing something, but if you try to model this in > web-forms you will end up with something different then a field > that must be updatable AND automatically calculated (upon each > round-trip) > > in both cases you mention I think you will have > - an editable field (1/ gratuity: a numeric field, percentage > format initially set at 10% and 2/ isDiplomat: a > boolean-field/checkbox initially set OFF) > > next to but separate from > - a calculated field that shows the total. > > making sense? Thanks for the response. Every programmer can found a way to use the given tools to solve a problem. I know this can be solved as you show up. This is one posibility. But, I saw that we need another 2 fields that increse the complexity of the form. In the concrete restaurant example if you have only 2 cashier that need to register 300 invoices between 10:00 p.m to 2:00 a.m on Friday and Saturday. you will see that just "jumping" in another 2 fields make sense. This is why (by the chashier request, we implemented this). 2 kestrokes * 300 is 600 keystrokes saved at a day => less work! Best Regards, Antonio Gallardo > > -marc= > -- > Marc Portier http://outerthought.org/ > Outerthought - Open Source, Java & XML Competence Support Center > Read my weblog at http://radio.weblogs.com/0116284/ > email@example.com email@example.com
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The boron-oxygen core of borinate esters is responsible for the store-operated calcium entry potentiation ability
Store-Operated Calcium Entry (SOCE) is the major Ca2+ ion entry pathway in lymphocytes and is responsible of a severe combined immunodeficiency (SCID) when deficient. It has recently been observed or highlighted in other cell types such as myoblasts and neurons, suggesting a wider physiological role of this pathway. Whereas Orai1 protein is considered to be the channel allowing the SOCE in T cells, it is hypothesized that other proteins like TRPC could associate with Orai1 to form SOCE with different pharmacology and kinetics in other cell types. Unraveling SOCE cell functions requires specific effectors to be identified, just as dihydropyridines were crucial for the study of Ca2+ voltage-gated channels, or spider/snake toxins for other ion channel classes. To identify novel SOCE effectors, we analyzed the effects of 2-aminoethyl diphenylborinate (2-APB) and its analogues. 2-APB is a molecule known to both potentiate and inhibit T cell SOCE, but it is also an effector of TRP channels and endoplasmic reticulum Ca2+-ATPase.
A structure-function analysis allowed to discover that the boron-oxygen core present in 2-APB and in the borinate ester analogues is absolutely required for the dual effects on SOCE. Indeed, a 2-APB analogue where the boron-oxygen core is replaced by a carbon-phosphorus core is devoid of potentiating capacity (while retaining inhibition capacity), highlighting the key role of the boron-oxygen core present in borinate esters for the potentiation function. However, dimesityl borinate ester, a 2-APB analogue with a terminal B-OH group showed an efficient inhibitory ability, without any potentiating capacity. The removal or addition of phenyl groups respectively decrease or increase the efficiency of the borinate esters to potentiate and inhibit the SOCE. mRNA expression revealed that Jurkat T cells mainly expressed Orai1, and were the more sensitive to 2-APB modulation of SOCE.
This study allows the discovery of new boron-oxygen core containing compounds with the same ability as 2-APB to both potentiate and inhibit the SOCE of different leukocyte cell lines. These compounds could represent new tools to characterize the different types of SOCE and the first step in the development of new immunomodulators.
In lymphocytes, after T or B cell receptor cross-linking, inositol 1,4,5-trisphosphate (IP3) is synthesized
Due to the key role of Ca2+ influx in lymphocyte activation and proliferation, the use of effectors to modulate the Orai1-containing channels has appeared as a new and promising way to modulate lymphocyte activities and could represent a new way for the treatment of inflammatory diseases
One of the most interesting and promising molecule is a boron-containing molecule, 2-aminoethyl diphenylborinate (2-APB). 2-APB was originally described as a plasma membrane permeant inhibitor of IP3 receptors in human platelets and neutrophils
Recently, several works have been published on 2-APB analogues. Thus, several analogues of 2-APB (1; Figure
Figure 1
Structure of 2-APB and analogues
Structure of 2-APB and analogues. Three structures of 2-APB are depicted besides analogues: open-chain monomer, ring monomer when a ring is created by an N → B coordinate bond, dimeric form, when N → B bonds are created between two molecules of 2-APB.
The aim of this study was to identify which part of the 2-APB molecule is required for potentiation (and inhibition) of SOCE by using analogues modified in the three parts of the molecule: the BOC, the ethanolamine group and the phenyl rings. We show that the BOC is an absolute requirement for the potentiating ability of 2-APB (but not for the inhibitory capacity), and that the number of phenyl rings is linked to the capacity of the molecule to potentiate/inhibit. We also confirm that the ethanolamine group is not required for potentiation and inhibition of SOCE in other cells.
Dual effects of borinate esters (2-APB, DPBA and 2-ABB) on SOCE
To visualize the SOCE, we treated the cells with 1 μM thapsigargin (TG) in a Ca2+-free medium during 10 min: this treatment allows the release of Ca2+ ions from the ER (Figure
Figure 2
Borinate esters have dual effects on store-operated calcium entry
Borinate esters have dual effects on store-operated calcium entry. A. Cytosolic calcium concentration measurement of BL41 cells using indo-1 fluorescence. Cells were treated 10 min with TG 1 μM to allow Ca2+ release from ER and opening of SOC channels. After 10 min, 1 mM CaCl2 was re-added, allowing Ca2+ entry through SOC channels. Different 2-APB concentrations were applied 30 s prior to CaCl2. Error bars were omitted for clarity (maximum ~ ± 70 nM). B. Mn2+ quenching of indo-1 in BL41 cells stimulated by potentiating and inhibitory concentrations of 2-APB. Cells were pre-treated 10 min with TG 1 μM allowing Ca2+ release from ER and opening of SOC channels. 60 s after beginning of the recordings, 100 μM MnCl2 was added. At 120 s, potentiating concentration of 2-APB (10 μM) was added, followed 60 s later by inhibiting concentration of 50 μM. Fluorescence of indo-1 was recorded at 430 nm. Results expressed relative fluorescence prior to MnCl2 adding. C. 2-APB, DPBA and 2-ABB concentrations differently regulate SOCE of BL-41 cells. Experiments were done as in figure 1A, and peak [Ca2+]i was expressed as % of peak [Ca2+]i recorded in absence of any borinate ester compounds. Borinate ester compounds were added 30 s prior to CaCl2. D. Mn2+ quenching of indo-1 in BL41 cells stimulated by potentiating and inhibitory concentrations of DPBA. Same as in figure 2B, except that 3 and 50 μM DPBA were used. E. Mn2+ quenching of indo-1 in BL41 cells stimulated by potentiating and inhibitory concentrations of 2-ABB. Same as in figure 2B, except that 30 and 100 μM 2-ABB were used. F. Values of Indo-1 quenching in presence of the borinate esters were normalised to values obtained in the lonely presence of Mn2+ ions.
These results were confirmed by indo-1 quenching experiments with Mn2+ ions: as Mn2+ ions enter the cells by the same channels as Ca2+ ions, they can not be pumped back to the external medium by the plasma membrane Ca2+ ATPases (PMCA) and lead to indo-1 fluorescence quenching at 430 nm
Diphenylborinic anhydride (DPBA) and 2-aminoethyl dibutylborinate (2-ABB, 4; Figure
2-ABB, another 2-APB analogue where the two phenyl rings are replaced by two butyl groups, was further shown to have a dual effect on BL41 cell SOCE (Figure
Differential effects of dimesitylborinic acid (DMBA) and phenylboronic acid (PBA) on SOCE
As diphenylborinic acid is unstable, we chose dimesitylborinic acid (DMBA, 5; Figure
Figure 3
Non-borinate esters are either uneffective or inhibitors of SOCE
Non-borinate esters are either uneffective or inhibitors of SOCE. Dose-response curve of [Ca2+]i to 2-APB analogues were obtained as in figure 2C. A. DMBA, DPTHF and DP3A are inhibitors of SOCE. B. Mn2+ quenching of indo-1 in BL41 cells stimulated by two concentrations of DMBA or 2-APB. Same as in figure 2B, except that 1 and 20 μM DPBA or 2-APB were used. C and D. Cytosolic calcium concentration measurement of BL41 cells in 2-APB/DMBA competition experiments. Cells were treated 400s with TG 1 μM to allow Ca2+ release from ER and opening of SOC channels. Then 1 mM CaCl2 was added, allowing Ca2+ entry through SOC channels. 2-APB 10 μM ("2-APB", C), DMBA 10 μM ("DMBA", D) or 2-APB 10 μM + DMBA 10 μM ("2-APB + DMBA", C and D) were applied 30 s prior to CaCl2. In competition experiments, DMBA 10 μM was applied 100 s after CaCl2 adding on cells treated by 2-APB ("2-APB then DMBA", C) or 2-APB 10 μM was applied 100 s after CaCl2 adding on cells treated by DMBA ("DMBA then 2-APB", D). Error bars were omitted for clarity (maximum ~ ± 50 nM). E. Diphenhydramine and phenytoine are weak inhibitors of SOCE, although PBA is inefficient. Same experiment as done in figure 3A. F. Mn2+ quenching of indo-1 in BL41 cells by low and high concentrations of DPTHF and DP3A, compared to 2-APB. Same protocol was used as in figure 2B. The two analogues were used at 10 μM and 50 μM. G and H. Values of Indo-1 quenching in presence of the borinate esters were normalised to values obtained in the lonely presence of Mn2+ ions, respectively from B and F.
Last we tested phenylboronic acid (PBA, 6; Figure
2-APB analogues without the B-O core are not able to potentiate SOCE
Thus, compounds containing a terminal B-OH (DMBA and PBA) do not appear to behave like compounds containing a central B-O ("BOC", 2-APB, DPBA and 2-ABB), suggesting a requirement of the BOC for the potentiating activity of 2-APB analogues. To confirm this hypothesis, we performed experiments with 2-APB analogues devoid of B-O. Several of these analogues (Phenytoin, diphenhydramine, DPTHF) have been tested on platelet SOCE and described as inhibitors
Diphenhydramine (7; Figure
2,2-diphenyltetrahydrofuran (DPTHF (3; Figure
3-(diphenylphosphino)-1-propylamine (DP3A (9; Figure
Dual effects of borinate esters (2-APB, DPBA and 2-ABB) are observed in other leukemia/lymphoma cell lines
As the most extensively studied SOCE is CRAC of T lymphocytes
Figure 4
Borinate esters have dual effects on SOCE of myeloid and lymphoid hematopoietic cells
Borinate esters have dual effects on SOCE of myeloid and lymphoid hematopoietic cells. Dose response curves for 2-APB (A), DPBA (B) and 2-ABB (C) obtained on SOCE of BL-41, U937 and Jurkat cells. Same protocol was used as in figure 2A to generate the dose-response curve of [Ca2+]i increase after Ca2+ re-adding to borinate compound concentrations. Values are expressed as % of the peak [Ca2+]i increase measured in absence of any effector.
DPBA was also 3 fold more efficient than 2-APB in Jurkat and U937 cells (Figure
2-ABB had the same dual effect on U937 and Jurkat cell SOCE (Figure
Orai isoform expression
SOCE is directly dependent on Orai protein expression. Three Orai isoforms have been characterized in human cells and exhibit different sensitivities to 2-APB when over-expressed in HEK 293 cells
Figure 5
Orai isoform mRNA are differently expressed in hematopoietic cells
Orai isoform mRNA are differently expressed in hematopoietic cells. qRT-PCR results were expressed as arbitrary units with expression of Orai3 in Jurkat equal 1. See material and methods for details.
Borinate esters are also Ca2+ release inhibitors
To test the SOCE-specificity of the 2-APB analogues, we performed the same kind of experiment, except that the cells were stimulated with an anti-IgM antibody instead TG. In these conditions, anti-IgM antibodies induce synthesis of IP3 and Ca2+ ion release through IP3 receptor of the ER
Figure 6
2-APB and analogues are Ca2+ release inhibitors
2-APB and analogues are Ca2+ release inhibitors. BL-41 cell cytosolic concentrations were measured as in figure 1. To induce Ca2+ release by the ER, cells were treated with 0.5 μg/ml anti-IgM antibody at t = 30 s. According to the treatment, cells were pre-treated with 2-APB or analogues during 5 min prior anti-IgM antibody stimulation. Error bars were omitted for clarity but were < 4 nM.
DMBA at 10 (not shown) and 20 μM was also able to totally block the Ca2+ release by the ER, in a range of concentration similar to concentration needed to block the SOCE (Figure
In this work, we clearly show that 2-APB is able to exert dual effects (potentiation and inhibition) on the Ca2+ influx of various cell lines of hematopoietic origin, and may be considered as the parent of a new family of molecules with this dual efficacy. We were able to define which part of the 2-APB molecule is required for potentiation and/or inhibition of the Ca2+ influx through store-operated channels. Despite the same kind of activity in hematopoietic cell lines, a slight difference in sensitivity to 2-APB and its analogues can be observed, and could be due to the relative expression of the three Orai proteins.
Though 2-APB is a small molecule, three parts can be distinguished: an ethanolamine chain, a boron-oxygen core (BOC) and two phenyl rings. The presence of a boron atom with an amine chain allows the formation of an internal coordinate, but the boron atom is also able to interact with N and O atoms of amino-acids, as shown in the case of bortezomib binding to proteasome
DPBA had been previously used and was known as a five fold more potent inhibitor of platelet SOCE than 2-APB (2 μM
2-ABB, a compound never used in Ca2+ signalling, is an analogue of 2-APB where the two phenyl groups were replaced by two butyl. On the three cell lines, we found that 2-ABB, like 2-APB and DPBA, has dual effect on Ca2+ influx, meaning that the two phenyl rings are not an absolute requirement for potentiation and inhibition processes. However, 2-ABB is less potent to potentiate AND inhibit SOCE than 2-APB and DPBA. A clear correlation seems to exist between the number of phenyl rings in the molecule and its ability to potentiate/inhibit SOCE: DPBA (4 phenyl groups) > 2-APB (2) > 2-ABB (0). Thus, it seems that the phenyl rings do not play a direct role in potentiation, but rather favour it. We can assess that after the binding of 2-APB to the channel protein by the BOC, the two phenyl rings strengthen the binding by a second interaction. In DPBA, the presence of four phenyl rings reinforces this binding. It was previously shown that addition of a phenyl ring to the 2-APB molecule could increase its inhibitory efficacy on platelet SOCE
It is also remarkable that the ratio between the concentrations of compound to have maximal potentiation, and to have inhibition is similar with the three borinate esters (from BL41 cells, but same kind from Jurkat and U937 cells): 2-APB 10 μM/100 μM, DPBA 3 μM/30 μM, 2-ABB 30 μM/200 μM (this last value was extrapolated from graphs): this clearly shows that potentiation and inhibition are linked. Interestingly, 2-ABB is the first described SOCE inhibiting molecule without any structural rings.
Sensitivity to potentiation/inhibition by borinate esters varies among cell line SOCE: thus, these compounds are three fold more potent on Jurkat cell SOCE. Using qRT-PCR, we showed the the cell lines used in this study do not express the same Orai proteins, and in different ratios (Figure
Since the molecular characterization of Orai1
The presence of a boron-oxygen link in a molecule does not mean that a compound has potentiating ability on SOCE. DMBA, which resembles 2-APB without the ethanolamine group but a terminal hydroxyl group bound to the boron atom, has shown great capacity to block SOCE, without any potentiating effect. The dipeptidyl boronate pyrazylcarbonyl-Phe-Leu-boronate, also known as PS-341 or bortezomib (commercial name: Velcade®) is a well known inhibitor of proteasome used in the treatment of multiple myeloma. Its pharmacophore is a terminal boron atom with two hydroxyl groups
The absolute requirement of the boron atom for potentiation was confirmed by DP3A. Despite its closely related structure to 2-APB', DP3A had never been used in Ca2+ signalling before this work. Remarkably, this compound where a P-C core replaces the BOC present in 2-APB, has lost its ability to potentiate SOCE of hematopoietic cell lines (Figure
The dual effect of borinate esters on SOCE is complex to analyse because the binding sites of 2-APB are not clearly established yet. Thus, before the discovery of Orai1, the study of the T cell SOCE, characterized the dual effect of 2-APB and the authors hypothesize the existence of two binding sites, one potentiating with high affinity, the other one with lower affinity and inhibitory
However, all the compounds used in this study are not SOCE specific, as they also exert ability to inhibit the Ca2+ release by the ER. Thus, when borinate esters are at their maximal potentiating concentration, they are already at a concentration fully inhibiting the Ca2+ release (Figure
The results from the present study clearly highlight the role of the Boron-Oxygen core in the ability of borinate ester molecules to potentiate Ca2+ entry in hematopoietic cells. The design of potentiating compounds requires the absolute presence of a BOC, whereas design of inhibitors does not. Strikingly, terminal B-OH could give high inhibitory ability to molecules (DMBA). This work could represent the first step for the development of new molecules with immunomodulatory properties: thus, a potentiating compound could be useful to enhance responses of blood cells from immunodeficient patients, or to increase Ca2+ toxicity in leukemia cells.
Cell lines
The BL30 and BL41 (Burkitt B lymphomas), Jurkat (acute T cell leukemia), U937 and THP1 (monocytic cell lines) and NB4 (promyeloid leukaemia cell line) cell lines were maintained in RPMI-1640 medium (Lonza, Verviers, Belgium) supplemented with 10% fetal calf serum and 2 mM ultraglutamine (Invitrogen, Cergy-pontoise, France), at 37°C in a 5% CO2 humidified atmosphere. All cell lines used were from ATCC-LGC Promochem (Molsheim, France) except BL-30 and BL-41 cells which were a gift of Irene Joab
Measurement of intracellular Ca2+ concentration ([Ca2+]i)
The intracellular Ca2+ concentration was recorded by a fluorimetric ratio technique. Cells were centrifugated and resuspended in Phosphate Buffer Saline medium (PBS, Cambrex, Belgium) supplemented with 1 mg/ml Bovine Serum Albumine (Sigma, Saint Quentin Fallavier, France) under gentle agitation. The fluorescent indicator indo-1 was loaded by incubating the cells at room temperature for 1 h with 4 μM indo-1-AM (Invitrogen/Molecular Probes, Eugene, USA). Cells were then spinned and resuspended in Hepes Buffer Saline (HBS) medium without CaCl2 (in mM): 135 NaCl, 5.9 KCl, 1.2 MgCl2, Hepes 11.6, glucose 11.5, pH 7.3 adjusted with NaOH.
One million cells were put in a 1 cm width - 3 ml quartz cuvette, and inserted in a spectrofluorophotometer (RF-1501 Shimadzu Corporation, Japan), equipped with a thermostatted (37°C) cuvette holder. Ultraviolet light of 360 nm was used for excitation of indo-1, and emission at 405 and 480 nm were recorded. Background and autofluorescence of the cells were removed from the values measured at 405 and 480 nm. The maximum indo-1 fluorescence (
To induce SOCE, the cells were treated for 10 min with 1 μM TG in the absence of external Ca2+. After 10 min, 1 mM CaCl2 was added. For dose-response curves, the peak [Ca2+]i was measured and expressed as a percentage of peak [Ca2+]i measured in absence of the test compound. These experiments were done at least three times, but for clarity, the SEM was not shown on traces, but is less than 1-2% of the mean.
To calculate the Ca2+ influx rate (Δ[Ca2+]i), we measure the variation of [Ca2+]i per second during the first 20 s after CaCl2 addition: in these conditions, the activities of the plasma membrane Ca2+ ATPases (PMCA) and of the Na+/Ca2+ exchanger are largely reduced (and the activity of SERCA is already inhibited by TG). Thus, the Δ[Ca2+]i is only due to the Ca2+ ion entry
Mn2+ ion binding to indo-1 forms a non-fluorescent complex. Thus, Mn2+ entry can be estimated by the Indo-1 fluorescence quenching rate
To test the effects of 2-APB and its analogues on the Ca2+ release by the ER, the BL41 cells were stimulated by an anti-IgM antibody (ABCam, Paris, France) instead by TG, still in absence of extracellular Ca2+. This kind of antibody cross-links to the B cell receptor induces the synthesis of IP3 and Ca2+ release through IP3 receptors
Orai isoform expression
Total RNA was isolated from cell lines by using the TRIzol® Reagent (Invitrogen) following the manufacturers' instructions. RNA was quantified with Thermo Scientific NanoDrop™ Spectrophotometers. Reverse transcription was performed on 1 μg of total RNA. After 3 min at 95°C, RNA was incubated for 1 h at 42°C with 50 units of M-MLV reverse transcriptase (Applied Biosystems), 20 units of RNase inhibitor (Applied Biosystems), 2.5 mM random hexamers and dNTP at 1 mM in a final volume of 20 μl. Finally, reverse transcriptase was denatured by heating for 5 min at 95°C. We quantified transcripts of the
GAPDH-Forward: GGACCTGACCTGCCGTCTAGAA
GAPDH-Reverse: GGTGTCGCTGTTGAAGTCAGAG
ORAI1-Forward: TCGGTCAAGGAGTCCCCCCAT
ORAI1-Reverse: GTCCTGTAAGCGGGCAAACTC
ORAI2-Forward: GCTGAGCTTAACGTGCCTATC
ORAI2-Reverse: GGAGTTCAGGTTGTGGATGTT
ORAI3-Forward: CCCTTAGTCCAGCTTCCAATC
ORAI3-Reverse: CCAAGGAGCGGTAGAAATGCA
PCR was performed using an ABI Prism 7700 Sequence Detection system (Perkin-Elmer Applied Biosystems) and the SYBR® Green PCR Master Mix (Applied Biosystems). The thermal cycling conditions comprised an initial denaturation step at 95°C for 10 min and 40 cycles at 95°C for 15 s and 60°C for 1 min. Experiments were performed in duplicates for each data point.
2-aminoethoxydiphenyl borate (2-APB), 2-aminoethoxydibutyl borate (2-ABB), diphenylboronic anhydride (DPBA), 2,2-diphenyltetrahydrofuran (DPTHF), phenylborinic acid (PBA), 3-(diphenylphosphino)-1-propylamine (DP3A), phenytoin, diphenhydramine, ,dimesitylborinic acid (DMBA) and 2-aminoethyl dibutylborinate (2-ABB) were from Sigma-Aldrich (Saint Quentin Fallavier, France), thapsigargin was purchased from Calbiochem-Merck (Nottingham, UK). The structure of 2-APB and analogues is given in Figure
Statistical analysis
Given values are representative of at least 3 independent experiments ± SEM. When used, a t-test < 0.05 is considered as significant.
List of abbreviations
SOCE: Store-operated calcium entry; SOC: Store-Operated Channel; CRAC: Calcium-Release Activated Calcium; IP3: inositol 1,4,5-trisphosphate; 2-APB: 2-aminoethoxydiphenylborate (2-aminoethyl diphenylborinate); [Ca2+]i: intracellular Ca2+ concentration; ER: endoplasmic reticulum; TRP: transient receptor potential; BOC: boron-oxygen core; TG: thapsigargin; DPBA: diphenylborinic anhydride; DPTHF: 2,2-diphenyltetrahydrofuran; PBA: phenylborinic acid; DP3A: 3-(diphenylphosphino)-1-propylamine; DMBA: dimesitylborinic acid; 2-ABB: 2-aminoethyl dibutylborinate.
Authors' contributions
OD carried out the [Ca2+]i measurements and Orais expression assays, and drafted the manuscript. PM carried out some [Ca2+]i measurements and compound tests.
CC conceived and designed the study. All authors have approved the final manuscript
The author would like to thank Patricia Krief for her technical help and Béla Papp for helpful talks. This work was financially supported by Association pour la Recherche sur le Cancer and Ligue contre le Cancer (France).
Inositol trisphosphate and calcium signaling
Ca2+/calcineurin signalling in cells of the immune system
Inhibition by SK&F 96365 of Ca2+ current, IL-2 production and activation in T lymphocytes
A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function
Amplification of CRAC current by STIM1 and CRACM1 (Orai1)
The molecular choreography of a store-operated calcium channel
Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1
Biochemical and functional characterization of Orai proteins
CRAC inhibitors: identification and potential
New therapeutic targets in immune disorders: ItpkB, Orai1 and UNC93B
Small-Molecule Inhibitors of Store-Operated Calcium Entry
2APB, 2-aminoethoxydiphenyl borate, a membrane-penetrable modulator of Ins(1,4,5)P3-induced Ca2+ release
Inhibition of SERCA Ca2+ pumps by 2-aminoethoxydiphenyl borate (2-APB). 2-APB reduces both Ca2+ binding and phosphoryl transfer from ATP, by interfering with the pathway leading to the Ca2+-binding sites
2-Aminoethoxydiphenyl borate affects the inositol 1,4,5-trisphosphate receptor, the intracellular Ca2+ pump and the non-specific Ca2+ leak from the non-mitochondrial Ca2+ stores in permeabilized A7r5 cells
2-Aminoethoxydiphenyl borate (2-APB) antagonises inositol 1,4,5-trisphosphate-induced calcium release, inhibits calcium pumps and has a use-dependent and slowly reversible action on store-operated calcium entry channels
2-aminoethoxydiphenyl borate reveals heterogeneity in receptor-activated Ca(2+) discharge and store-operated Ca(2+) influx
Potentiation and inhibition of Ca(2+) release-activated Ca(2+) channels by 2-aminoethyldiphenyl borate (2-APB) occurs independently of IP(3) receptors
Modification of store-operated channel coupling and inositol trisphosphate receptor function by 2-aminoethoxydiphenyl borate in DT40 lymphocytes
2-aminoethoxydiphenyl borate activates and sensitizes the heat-gated ion channel TRPV3
2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, and TRPV3
Mechanism of inhibition of TRPC cation channels by 2-aminoethoxydiphenylborane
2-aminoethoxydiphenyl borate as a prototype drug for a group of structurally related calcium channel blockers in human platelets
2-Aminoethoxydiphenyl borate directly inhibits store-operated calcium entry channels in human platelets
2-Aminoethyl diphenylborinate analogues: selective inhibition for store-operated Ca2+ entry
Synthesis of bisboron compounds and their strong inhibitory activity on store-operated calcium entry
Inhibition of the calcium release-activated calcium (CRAC) current in Jurkat T cells by the HIV-1 envelope protein gp160
Methods for studying store-operated calcium entry
CRACM1, CRACM2, and CRACM3 are store-operated Ca2+ channels with distinct functional properties
Genetic evidence for involvement of type 1, type 2 and type 3 inositol 1,4,5-trisphosphate receptors in signal transduction through the B-cell antigen receptor
Active site-directed inhibitors of Rhodococcus 20 S proteasome. Kinetics and mechanism
Biphasic currents evoked by chemical or thermal activation of the heat-gated ion channel, TRPV3
Pharmacological characterization and molecular determinants of the activation of transient receptor potential V2 channel orthologs by 2-aminoethoxydiphenyl borate
Gene regulation mediated by calcium signals in T lymphocytes
A novel native store-operated calcium channel encoded by Orai3:Selective requirement of Orai3 versus Orai1 in estrogen receptor positive versus estrogen receptor negative breast cancer cells
TRPC1: the link between functionally distinct store-operated calcium channels
Orai proteins interact with TRPC channels and confer responsiveness to store depletion
Store-operated Ca(2+) entry in platelets occurs independently of transient receptor potential (TRP) C1
Orai1 and STIM reconstitute store-operated calcium channel function
Properties of Orai1 mediated store-operated current depend on the expression levels of STIM1 and Orai1 proteins
Bortezomib (PS-341): a novel, first-in-class proteasome inhibitor for the treatment of multiple myeloma and other cancers
Proteasome inhibitors: from research tools to drug candidates
Potent and selective inhibitors of the proteasome: dipeptidyl boronic acids
Strategies for the inhibition of serine proteases
STIM1 has a plasma membrane role in the activation of store-operated Ca(2+) channels
2-Aminoethoxydiphenyl borate directly facilitates and indirectly inhibits STIM1-dependent gating of CRAC channels
Modulation of B-cell endoplasmic reticulum calcium homeostasis by Epstein-Barr virus Latent Membrane Protein-1
A new generation of Ca2+ indicators with greatly improved fluorescence properties
Phorbol ester-induced changes in cytoplasmic Ca2+ in human neutrophils. Involvement of a pertussis toxin-sensitive G protein


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This property has been removed by the agent. It may be sold or temporarily removed from the market
2 bedroom terraced house for saleEagle Street, Hanley, Stoke-On-Trent
Removed £59,950
Property Description
Key features
- Pre-let Investment - Rental income £395 PCM
- Gas Central Heating, Majority Double Glazing
- Two Reception Rooms
- No Upward Chain
- Energy Performance Band D, Rating 57
- Please Ask One of Our Advisors For Further Details
Full description
A pre-let investment property, with a rental income of £395 per calendar month. The property is a two bedroom terraced house, with gas central heating and majority double glazing, close to the amenities of Hanley city centre. Living accommodation comprises two reception rooms, kitchen and bathroom at ground floor level, with two bedrooms to the first floor. Please see full details for tenancy information.
More information from this agent
To view this media, please visit the on-line version of this page at www.rightmove.co.uk/property-for-sale/property-42569424.html
Energy Performance Certificates (EPCs)
To view this media, please visit the on-line version of this page at www.rightmove.co.uk/property-for-sale/property-42569424.html
Listing History
Map & Street View
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Market Info
Disclaimer - Property reference 6804137. The information displayed about this property comprises a property advertisement. Rightmove.co.uk makes no warranty as to the accuracy or completeness of the advertisement or any linked or associated information, and Rightmove has no control over the content. This property advertisement does not constitute property particulars. The information is provided and maintained by Keates Hulme, Longton. Please contact the selling agent or developer directly to obtain any information which may be available under the terms of The Energy Performance of Buildings (Certificates and Inspections) (England and Wales) Regulations 2007 or the Home Report if in relation to a residential property in Scotland.
Map data ©OpenStreetMap contributors.


text:
Download omniture-7.x-1.x-dev.tar.gztar.gz 14.44 KB
MD5: c0788a9f4262d05efcb96def54a56fc9
SHA-1: ea9d756a5ceb14dc524562af85e5e9661418834d
SHA-256: 43dcdcf509bb69e9484164d05ebf81b65302230ac3ee84258bfed670597f1111
Download omniture-7.x-1.x-dev.zipzip 17.11 KB
MD5: 030b12532fb39aa43f429c472c97bb3f
SHA-1: bd2403cd96180a49504aa29893b1426d254bc684
SHA-256: d5b31030072be3caec65480fe72cfa6089342676875504bea4e70e6c8e02b6c8
Release info
Created by: Race.it
Created on: 11 Feb 2010 at 13:05 UTC
Last updated: 12 Feb 2010 at 00:12 UTC
Core compatibility: 7.x
Release type: New features
Release notes
This is a D7 version of the omniture module that has been refactored by Pasquelle, some of the functionality has been moved into a sub module omniture_basic, that deals with the taxonomy tracking and populating values into the omniture javascript variables.
The selected release is the release that will be used for automated testing. Optional projects are only used for testing.
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text:
Long story short, my boyfriend and I were supposed to hang out yesterday and I would meet him at his job. He texts me what time I would be there, and I simply told him that I don't know but that it shouldn't concern you since I'll be there. He starts to get snappy and began making smart ass comments. I pointed out his attitude. He tells me he doesn't have an attitude, and warns me to keep pushing it and I'll get one. I laughed. He tells me don't bother showing up. He doesn't text me all day. I text it before going to bed as a way to maybe tall about it, but doesn't respond. After waking up, he asks like nothing happened. I don't know if I should go to his job to confront him, call him later or just let it be. I feel wronged, and we haven't seen reach other in a while. The day he decides to cancel plans is the day we have one chance to hangout.
Should I confront him, bring it up or let it be?
Sorry for the typos, by the way.
Have an opinion?
What Guys Said 2
this is a weird situation. it seems petty. talk to him but don't make a bigger deal than what it already is.00|00|0
Yeah, I'll try. Only if he doesn't blow it out of proportion.
- Show AllShow Less
well when a guy is in this state, best to give him time to cool down. he's only thinking off of angry right now.
Yeah, you're right.
Confront this.00|00|0
I'll try.
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text:
New product
Pool heater
Pool heater
The Sun, for Intex is not the only method of heating for swimming pools, in fact offers an electric heater that safely allows to increase a few degrees the water temperature of your pool. The heater for above ground pools from 2.44 m to 4.57 m in diameter, up to 12 m includes 230 volt cable for easier installation 3.0 Kw, a sensor that will automatically turn off the appliance as soon as the water reaches the ideal temperature, falicissimo for use with two simple buttons that set the switching on and off.
Tracking of Shipping: As soon as your item is shipped, you will receive an email with a link to the monitoring of the shipment.
With this system you can know the status and location of delivery.
Timing of packaging and shipping: The item is shipped the same day payment * at the latest, the following day. Do not count as working days: Saturday, Sunday and all those days on the calendar that are in red. * (valid for payments received through PayPal or other methods immediate. Payments must be received by 13:00 on the days between Monday to Friday)
The cost for shipping varies depending on the product placed in the shopping cart
a mino € 7.00 to a maximum € 25.00
The shipping is free for orders over € 150 EU
The shipping is free for orders over € 300 Out EU
The times for shipping varies from 2-9 days
To conclude a purchase with this type of payment you will see a page of our website where you can enter 'e-mail account and password of your PayPal account or access to the procedure for creating a new one.
SOFORT Online Wire Transfer
SOFORT Banking. The system of direct bank transfer, international and TÜV.
The high safety standards of SOFORT Banking minimize the risks associated with online payments. A payment system verified and data protection verified by TÜV Saarland. Payments online with ease and security!
Each transaction requires the entry of personal home banking known only to the User himself (please keep handy the details of their bank account and PIN).
Each observation is confirmed and approved by the user with a password dispositive, an authorization code or by other confirmation procedures.
This payment does not include costs aggiuntivi.Sarete redirected to the payment after order confirmation.
Valid only for the following countries (Austria, Belgium, France, Germany, Italy, Netherlands, Poland, Switzerland)
Bank transfer
Payment system is not on-line, using this method will see the page that shows the data required to make the payment. Not being an immediate payment method, the seller will wait until the actual crediting of 'amount provided before starting the shipping process.


text:
Kobe Bryant: Kobe Bryant's Masked Revenge
March 2, 2012 by staff
Kobe Bryant: Kobe Bryant's Masked Revenge, Kobe Bryant doesn't forget about anything that happens to him on a basketball court.
The Black Mamba never shrinks from a challenge, and thrives off anything he can use as motivation to enhance his game.
There is no question that Dwyane Wade's foul on Bryant in the All-Star Game was a hard one, but it would be foolish to think that the Miami Heat star had intentions of injuring Bryant on the play.
According to ESPN.com's Tom Haberstroh, Wade has apologized to Bryant for the outcome of the play.
"It's all I can do," Wade said of the message to Kobe. "He knows it's no ill intent of me to do that to him. Talk about me for taking the foul, but I never wanted that kind of outcome."
Despite the apology, some of Bryant's teammates were less than thrilled with Wade's on-court actions.
"It was an All-Star Game," said Andrew Bynum, who was on the Western Conference All-Star's bench in Orlando at the time of the foul. "I don't understand what that was all about. It was crazy."
Added Pau Gasol: "I think it was out of place, out of line, for the moment and the game that it was, but I don't think he intended to break his nose. He just fouled him kind of hard there and got his nose. But again, I don't think it was the place to foul like that."
Bryant insists that he holds no ill will toward Wade.
"It's always entertaining to me to hear people talk about our relationship as if they know really what's going on and try to say there's something between them and all this other stuff.
It was very simple. He didn't mean to do it. It's just something that just happens. He's not that type of person."
Please feel free to send if you have any questions regarding this post , you can contact on
Disclaimer: The views expressed on this site are that of the authors and not necessarily that of U.S.S.POST.


text:
IBISS, Brazil
The project fund for the International Children's Peace Prize 2008 funded two projects against social exclusion of children and youngsters in the favelas (slums); "Favela Força on tour" and "Vila Cruzeiro on-line".
Mayra's (Children's Peace Prize winner 2008) theatre group, Favela Força, makes its own productions around themes that are current for children and youngsters who live in the favela Vila Cruzeiro. By means of theatre, children and young people are informed and educated about their rights.


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- Rapid pass-thru cord access - Allow easy power cord access for battery charging
- Flexi-shelf - 8" adjustable bin storage
- Permanently retained lock with recessed shackle - Saves time, prevents lost locks and access by saws, pry bars and cutters (Lock not included)
- Fully arc welded construction - Tighter tolerances en sures durability
- Overlapping cover design - Superior weather resistance and security
- Durable baked enamel finish - Provides heavy-duty, long lasting protection
- Corner located lock housing - Prevents corner pry-ins; maximizes internal storage and access
- Solid lock tang - For superior security and pry resistance
- Ergonomic, recessed handles - 90-degree stop provides maximum leverage
- Full-length cover handle - Slip resistant, easy to grip along entire cover length
- Locking cover support - Secures cover in open position, prevents accidental closure
- Solid 1-piece 12 ga. skids - With wide base for greater strength and stability
|Arc Welded, 16 Ga. Steel
|(48" x 24" x 28")
|Usable storage
|16 Cu. Ft.
|Shipping weight
|149 lbs.
Support Documents
2048 On-Site Storage Chest is rated out of 5 by 63.
Rated 1 out of 5 by robertsa from Leaks in the rain and no support for missing parts I wrote customer service last week because I'm missing the tray inside and received no response. It also leaks when it rains which I was told it wouldn't do. The locks are a little funky but work well once adjusted just right. I own other Ridgid tools that I like very much so this is disappointing.
Date published: 2017-08-14
Rated 3 out of 5 by prop from storage box My box arrived badly dented, and the left side is really difficult to get the padlock locked, the locking arm is set too far forward.
Date published: 2017-07-19
Rated 4 out of 5 by Trayman from Help help help flexi tray Love my box but it didnt come with the flexi tray an before put into service i need that tray for it. I can make on but im about to start buying everything ridgid an i can even get this tray the has the dividers...? Someone please help!!!!
Date published: 2017-06-14
Rated 5 out of 5 by tedsesr from You Got To Buy It! This storage chest is by far is the best bang for your dollar ; it's everything they say it is and more... don't hesitate - just go out and buy!
Date published: 2017-06-13
Rated 5 out of 5 by JeffreLee from This box is very roomy. I have this gangbox mounted in the back of my truck in place of a tool box. It has out done my expectations. It's subjected to water/rain I've had no leaks or I'll effects. The 2048 has been in my truck just over a year now.
Date published: 2017-05-13
Rated 4 out of 5 by Tradesman from Seems to be a great product, can easily go from job site to the back of your truck for quick transit. I like how weather tight the lid closes. This 2048 size box is my primary choice for quick jobs. I use the #5 style combo locks on all job boxes so none of my workers have to worry about keeping up with keys. I installed 2 battend powered bright LED lights with notion sensors so you can see your tools in low light.
Date published: 2017-05-04
Rated 4 out of 5 by WoodyWoodpecker from Great box. The box is a little tall for a small pickup but just right for a full size pickup. This would be a great solution for someone that the over the bed typical toolbox just doesn't cut it. Loads in and out easily and could be left at the jobsite for safe tool storage.
Date published: 2017-04-19
Rated 5 out of 5 by Cliftonjosh from Awesome jobsite box and does the job. Love this box. Plenty of room and great storage. The eels look like quality compared to all the boxes I looked at. The only think I wish is that there was another tray other than the plastic one. Maybe a metal one that sits a little lower. Other than that, thumbs up!
Date published: 2017-03-29
Which casters can I buy with this unit? What is the bolt spacing for the casters?
The spacing for the bolt placement on the casters from center of hole to center of hole 3" x 3".
Date published: 2015-07-22
Where can I purchase the pieces that hold the locks in place?
Try your local hardware store or contact RIDGID, they will have them.
Date published: 2015-12-04
Can I get an additional orange flexi shelf for the 2048-OS JOB BOX?
The part number is 7650 but please take note that a 2048-OS cannot accommodate 2 Flexi-Shelf's as the box is designed that way. Thanks, RIDGID
Date published: 2014-06-03
Internal dimensions?
I assume the listed dimensions (48" x 24" x 28") are the external measurements. What are the internal dimensions of this storage chest?
Thank you for your interest in RIDGID! Please see attached images for your perusal. Regards, RIDGID
Date published: 2016-09-28
What are size of wholes on the skids ? So I can secure my box to a truck bed.
Thank you for your interest in RIDGID. Each skid foot will have 4 square holes that are 7/16" and 2 round holes that are 5/8". Regards, RIDGID
Date published: 2016-03-09


text:
Average White Band at Birchmere in Alexandria, VA on Apr 22, 2017
More InfoAverage White Band
Date: Saturday, April 22nd, 2017
Time: 7:30 pm
Address: (map)
3701 Mt Vernon Ave
Alexandria, VA 22305
United States
Phone: (703) 549-7500
The cheapest ticket option is usually the primary ticket seller, but sometimes you can find tickets below face value through secondary ticket sellers.
SuperStar Tickets is a Zumic preferred ticket broker for premium concert tickets. Use discount code SHOPCONCERTS for 5% off your order.


text:
A Star Is Born 4-Week Christmas Children's Ministry Curriculum
A Star Is Born Christmas Curriculum - This Christmas, Follow the Star!
The Wise Men followed a star to find the baby Jesus. But they knew, the true star was Jesus himself – the Son of God who came to bring us light and life. Kids will study 4 Bible lessons about the Christmas story in these 4 lessons leading up to Christmas. They will learn about the prophecy about Jesus in Isaiah, all the way to the story of Simeon who had been promised that he would live to see the Messiah born.
A Star Is Born Christmas Children's Ministry Curriculum Lesson Overview:
Memory Verse: "For to us a child is born, to us a son is given, and the government will be on his shoulders. And he will be called Wonderful Counselor, Mighty God, Everlasting Father, Prince of Peace." -Isaiah 9:6 (NIV)
Darkest Night – Thousands of years ago, Isaiah described the coming Messiah as a great light for the people walking in darkness. That light – that star – would be Jesus, the Son of God. Isaiah 9:2-7, For To Us A Child Is Born
Shining Bright – From the start, the light of Jesus shone so bright. Elizabeth's baby leapt for joy when Elizabeth met with Mary, who was pregnant with Jesus. Even then, some were able to recognize that Jesus was the light of the world. Luke 1:39-48, Mary Visits Elizabeth
Beautiful Light – A star in the sky drew the Wise Men to the tiny town of Bethlehem where they found baby Jesus. Today, the Holy Spirit draws us to the light of Jesus. Matthew 2:1-12, The Wise Men Visit Jesus
Wonderful Sight – Simeon and Anna beheld a wondrous sight when baby Jesus was brought to the temple to be dedicated. They recognized that Jesus was the light that would shine for all people – Jews and Gentiles. Luke 2:21-40, Jesus Presented in the Temple
Each Lesson Includes:
- Memory Verse
- Skit
- Object Lesson or Kids Sermon
- Large Group Lesson Outline
- Large Group Game
- Small Group Discussion Questions
- Make It Stick! Parent Sheet
- Graphics
About This Children's Ministry Curriculum:
- Instant Download
- Designed for Kids Ages 6-12
- 4-Week Series
- Includes Both Large Group and Small Group Resources
- Perfect for Children's Church or Sunday School
- We pin snack, craft, and game ideas on Pinterest for each of our Children's Ministry curriculum series'. Visit our Children's Ministry curriculum Pinterest board for this series.
Be sure to download our A Star Is Born Christmas Program that goes along with this curriculum:
Guaranteed to Be Awesome!
30-Day Money Back Guarantee. If you aren't doing cartwheels of joy because you love this Children's Ministry curriculum, we will give you a big hug and give you a 100% refund, no matter the reason. If you're not happy, we're not happy.
Find More Children's Ministry Curriculum:
Best-Selling Children's Ministry Curriculum
Newest Children's Ministry Curriculum


text:
WEBINAR: On-Demand
Learn How a Virtual Networking Approach Can Strengthen the Security of Federal Networks REGISTER >
Investor's Corner
Our Investor's Corner feature for this month is a report by Matt Barzowskas and Michael Prospero of FAC/Equities, titled "Managed Security Services: Outsourcing Security Expertise."
This in-depth analysis of the managed security services market lays out the market drivers and opportunities, categorizes the different types of security service providers and looks at the services each offers. The report also offers selection criteria for choosing a provider that's right for your organization and offers profiles of more than 40 providers.


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Recipe by kimbearly
a gooseberry patch recipe by request.
Top Review by Dwynnie
This was a good chili, though I had to make some modifications (my fault!) I used turkey instead of venison (as I didn't have any), added 1 can of beans (for DH), and added an extra can of diced tomatoes (to offset the beans) that had chipotle in them (in addition to the chipotles, as my chipotles were a little old!) The flavor was very good and not too spicy! Also, this made 6 servings for us (with mods). Great with cornbread! Thank you!
Ingredients Nutrition
- 1 lb ground round
- 1 lb ground venison
- 1 (16 ounce) packagesage flavored pork sausage
- 1 medium onion, chopped
- 2 cloves garlic, minced
- 1 large green pepper, chopped
- 2 (14 ounce) cans tomatoes with juice
- 1 (15 ounce) can tomato sauce
- 2 chipotle chiles in adobo, seeded and chopped
- 2 teaspoons adobo sauce
- 1 tablespoon chili powder
- 1 teaspoon cumin
- 2 teaspoons dried oregano
- 1⁄2 teaspoon salt
- shredded monterey jack cheese, to garnish
- sour cream, to garnish
- sliced green onion, to garnish
Directions See How It's Made
- in large dutch oven brown meats till crumbled.
- drain all but 1 tablespoon of drippings.
- saute onion, green pepper,and garlic about 10 minutes.
- add meat back to dutch oven.
- add tomatoes,tomato sauce,chipotles,adobo sauce and seasonings.
- stir well and simmer for at least 1 hour.
- serve with condiments of choice!


text:
Sat Nov 3, 2012, 05:46 PM
limpyhobbler (8,244 posts)
6 replies, 1114 views
Always highlight: 10 newest replies | Replies posted after I mark a forum
Replies to this discussion thread
|6 replies
Sam Seder: Occupy storm relief efforts (Original post)
|Nov 2012
|sabrina 1
|Nov 2012
|Nov 2012
|sabrina 1
|Nov 2012
|Nov 2012
|sabrina 1
|Nov 2012
|Nov 2012
Response to limpyhobbler (Original post)
Sat Nov 3, 2012, 06:05 PM
sabrina 1 (62,325 posts)
1. Sounds like they are doing great work.
Good for Sam Seder for helping out. A few of my friends used to live in the Rockaway area where they were very close to the water. I am glad they are getting into all these areas where there probably are elderly residents who need help.
What wonderful people they are.
Response to sabrina 1 (Reply #1)
Sat Nov 3, 2012, 06:19 PM
limpyhobbler (8,244 posts)
2. Yes.
It's a very moving story. The NYC Occupy people are really stepping up to help. They clearly have skills at coordinating a volunteer effort, running a volunteer kitchen, and managing donations and supplies. Much better for people to be organized and do something, instead of just helplessly waiting.
Response to limpyhobbler (Reply #2)
Sat Nov 3, 2012, 06:39 PM
sabrina 1 (62,325 posts)
3. I just watched Wolf Blitzer who was in one of the areas, I believe it was
Rockaway that OWS has been distributing supplies to. He spoke to a woman who stated that they have children who are hungry that there is no water etc. He made the comment that 'community organizations' are now there helping out. But he never mentioned OWS lol!
Response to sabrina 1 (Reply #3)
Sat Nov 3, 2012, 06:59 PM
limpyhobbler (8,244 posts)
4. ya I wouldn't expect him to in any case.
I'm sure lots of groups are helping out and working together. Thank goodness for people power.
Response to limpyhobbler (Reply #4)
Sat Nov 3, 2012, 07:17 PM
sabrina 1 (62,325 posts)
5. True, and he did not name any of them, so I guess it was unfair of me to jump
to conclusions.
I'm just glad they are all working together to get some help to those poor people. And good that he covered the story as that should get some more attention for them.
Response to sabrina 1 (Reply #5)
Sat Nov 3, 2012, 08:08 PM
limpyhobbler (8,244 posts)
6. I dunno
you might have been right, the 'Occupy Sandy' volunteers do seem to be playing a particularly big role. They might deserve some special recognition for being the first responders in many of these neighborhoods.


text:
"excellent service for new walking stick"
About: Bassetlaw / Community therapy Bassetlaw Community therapy
Posted by Impact998 (as ),
Excellent service, Victoria was so polite and informative. So very helpful.
Response from Lynda Potter, Hospice Team Leader, Bassetlaw Hospice, Bassetlaw Health Partnership 4 years ago
Thank you for taking the time to comment on the service you received from the Community Therapy Team.
The team work hard to make their service customer focused and I am glad that we were able to meet your needs.
I will pass on your positive comments to the team and to the individual member of staff involved.
Lynda Potter
- {{helpful}} of {{total()}} people think this response is helpful
- Was this response helpful? Yes | No
Thank you for your feedback.
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text: CONTACT INFORMATIONCREDENTIALSAWARDSPUBLICATIONSYuhchyau Chen, M.D., Ph.D.Cancer, Radiation OncologyClinical InterestsHead & Neck, Thyroid Cancer, Throat Cancer; Lung Cancer; Radiation OncologyContact InformationPhone NumbersAppointment: (585) 275-2171Fax: (585) 275-1531A member of the University of Rochester Medical Faculty GroupgroupAn Accountable Health PartnerassignmentAccepting New PatientsLocations111 Clara Barton StSte B194Dansville, NY 14437Radiation Oncology: (585) 335-8675Wilmot Cancer Institute601 Elmwood AveRochester, NY 14618Oncology: (585) 275-5823CredentialsFaculty AppointmentsRichard T. Bell Endowed Professorship in Radiation Oncology - Department of Radiation Oncology (SMD) SpecialtiesRadiation Oncology - American Board of RadiologyEducation1976BS | Taiwan - Non-Medical SchoolArts & Sciences1988PhD | Univ of WashingtonExperimental Pathology1990MD | Univ Washington Sch MedicineMedicinePost-doctoral Training & Residency07/01/1990 - 06/30/1991Internship in Internal Medicine at Virginia Mason Medical Center07/01/1991 - 06/30/1994Fellowship in Clinical Fellow at Harvard Medical School07/01/1991 - 06/30/1994Residency in Radiation Oncology at Massachusetts General HospitalVIEW ALL expand_moreAwards2014Educator of the YearSponsor: Association of Residents in Radiation Oncology2013 - PresentRichard T. Bell Endowed Professorship in Radiation OncologySponsor: Department of Radiation OncologyLocation: University of Rochester2012 - 2013Philip Rubin Professorship in Radiation OncologySponsor: Department of Radiation OncologyLocation: University of Rochester2012 - 2013FellowSponsor: Hedwig van Ameringen Executive Leadership in Academic MedicineLocation: Drexel University College of Medicine2011 - 2012Patient and Family Centered Care AwardSponsor: University of Rochester2001Educator of the YearSponsor: The Association of Residents in Radiation Oncology1997 - 1998Buswell Fellowship AwardSponsor: Department of MedicineLocation: University of Rochester1995 - 1996George Casarett Research Excellence AwardSponsor: Department of Radiation OncologyLocation: University of Rochester1993 - 1994Clinical Oncology FellowshipSponsor: American Cancer Society1986 - 1990Course HonorsSponsor: University of Washington School of Medicine1983 - 1984Graduate School Research AssistantshipLocation: University of Washington School of Medicine1982 - 1983Research Assistantship in SurgerySponsor: University of Washington School of MedicineVIEW ALL expand_morePublicationsJournal Articles7/7/2017Zhang F, Shen M, Yang L, Yang X, Tsai Y, Keng PC, Chen Y, Lee SO, Chen Y. "Simultaneous targeting of ATM and Mcl-1 increases cisplatin sensitivity of cisplatin-resistant non-small cell lung cancer." Cancer biology & therapy.. 2017 Jul 7; :0. Epub 2017 Jul 07. 6/1/2017Olsen JR, Moughan J, Myerson R, Abitbol A, Doncals DE, Johnson D, Schefter TE, Chen Y, Fisher B, Michalski J, Narayan S, Chang A, Crane CH, Kachnic L. "Predictors of Radiation Therapy-Related Gastrointestinal Toxicity From Anal Cancer Dose-Painted Intensity Modulated Radiation Therapy: Secondary Analysis of NRG Oncology RTOG 0529." International journal of radiation oncology, biology, physics.. 2017 Jun 1; 98(2):400-408. Epub 2017 Feb 13. 1/2017Wang X, Yang X, Tsai Y, Yang L, Chuang KH, Keng PC, Lee SO, Chen Y. "IL-6 Mediates Macrophage Infiltration after Irradiation via Up-regulation of CCL2/CCL5 in Non-small Cell Lung Cancer." Radiation research.. 2017 Jan 0; 187(1):50-59. Epub 2017 Jan 05. Books & Chapters2011 Chapter Title: Translational Research in Lung Cancer Book Title: Advances in Radiation Oncology in Lung Cancer Author List: Singh D; Bylund K; Chen Y Edited By: Jeremic B Published By: Springer-Verlag GmbH2011 in Heidelberg2006 Chapter Title: Molecular Staging of Non-small Cell Lung Cancer Book Title: Tumors of the Chest: Biology Diagnosis and Management Author List: Chen Y, Gandara D Edited By: Syrigos KN, Nutting C, Roussos C Published By: Springer-Verlag2006 in Berlin Heidelberg New York2005 Chapter Title: Translational Research in Radiation Oncology of Lung Cancer. Book Title: Advances in Radiation Oncology in Lung Cancer. Medical Radiology- Diagnostic Ima Author List: Chen Y Edited By: Jeremic B, Baert AL, Brady LW, Heilmann HP, Molls M, Sartor K Published By: Springer2005VIEW ALL PUBLICATIONS


text: Brocktoberfest by Current in Carmel · November 14, 2015 Clare Crumback and Molly Cason hold up their glasses at Brocktoberfest. (Photo by Theresa Skutt) Firefighter John Huseman looks on as firefighter Justin Cox signs his photo in a 2016 calendar featuring Hamilton Co firefighters. The calendars were for sale at Brocktoberfest. (Photo by Theresa Skutt) Mishell Martinez participates in the keg roll. (Photo by Theresa Skutt) Brocktoberfest, an annual mini beer festival at Brockway Pub in Carmel, featured local breweries, seasonal beers, a food truck, live music and a keg roll. Proceeds benefited Carmel firefighters. Share


text:
Significant social taboo is attached to discussing men's sexual health. In particular, Premature Ejaculation.
PE is devastating for a man's selfconfidence, and it can be hugely frustrating for his partner. We created an information-based campaign website, with TV support enabling customers to:
- Normalise discussions between couples and their GP
- Access GP supported materials
- Find non-judgemental advice and information
Within the first month, there were over 35,000 visitors to the site. In phase two, our learnings guided us towards creating specific content for women who were making up almost 50% of those visitors.


text:
The lawsuit will alleges Misrepresentations anout the school's Accreditation, Transferability of Credit, and Cost. See Miller,et al. v. Corinthian Colleges in Third Judicial District Court, Salt Lake Ciyt County.
Is this case about your college? Was the accredidation misrepresented? How would one prove it other than your word versus theirs?


text:
Mario Cantone 2017
Mario Cantone Concert dates
You are intended to attend Mario Cantone event as it comes close to your home. And of course, the event is appearing in 4 cities including Fort Lauderdale, Durham, Ridgefield, New York and more. Visit our website and get top Mario Cantone tickets at low prices unavailable within other websites. Cheap Mario Cantone tickets are our specialty, so profit of the chance!
Mario Cantone tour dates
Fort Lauderdale, FL
Durham, NC
Ridgefield, CT
New York, NY


text:
It's not a regular occurrence that you realize that you have to buy a replacement Door Mirrors to repair your car or truck. Anyone who operates a Mercedes-Benz knows that when it comes to maintaining their automobile only the absolute best replacement parts will do. The vehicle in your driveway is head and shoulders above the rest when it comes to all the cars, trucks, and SUVs these days. Mercedes-Benz vehicle owners have grown accustomed to a certain level of performance when driving their C240 around town.
There are a few parts on your vehicle that are both safety devices and convenience features. Your Mercedes-Benz C240 door mirrors give you the ability to see along both sides and behind your vehicle without cranking your neck every time you needed to look behind you. If your Mercedes-Benz is old enough, the factory may have only installed a door mirror on the left side; however most C240 models have door mirrors on both sides. The most heavily optioned Mercedes-Benz C240 models could possibly have door mirrors with power adjusting and power folding features, along with heated glass to remove frost.
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text:
Evergreen is an integrated library system originally developed by the Georgia PINES consortium for use as their automation system, and now includes contributions from around the world. It was designed from scratch for large-scale deployment in very large public library and state-wide consortium environments with tens of millions of records and hundreds of libraries, but can also scale down to the smallest of single-branch libraries.
Joomla contact form builder extension is a complicated tool for making submission forms for your Joomla website. It permits you to specify parameters like colors and fonts. It can use a Google map when given an address or geographical coordinates (longitude and latitude), and the user can drag the marker to the required location.
Keynesis Lockngo is a security application that runs directly from a portable drive. Lockngo encrypts and password-protects data against loss or theft. When protected, the drive appears empty (data is hidden) and will not allow new data to be written onto it. Lockngo has Military Grade 256-bit AES Encryption, FIPS 140-2 Certified. Lockngo also comes in a cross-platform version for Mac and Windows. This version allows you to lock a drive on a Macintosh and open it on a computer running Windows and vice versa.
Noosfero is a Web platform for social networks. Besides the regular social network features (such as friends and communities), Noosfero offers a full-featured CMS, making every user or community profile a complete Web site with several types of content (including text, image gallery, and file uploads), RSS feeds, agenda, the possibility to set custom themes, and other features. It is also possible to run several independent social networks within the same Noosfero installation.
Roundcube Webmail is a browser-based, multilingual IMAP client with an application-like user interface. It provides the full functionality you expect from an email client, including MIME support, address book, and folder manipulation. High extensibility is granted by the plugin API and the user interface is fully skinnable using XHTML and CSS 2.
A MS Excel spreadsheet component for Android.
A GTK-based Web development editor.


text:
Accessibility Whack-A-Mole · An A List Apart Article
A fascinating piece by Eleanor on the typographic tweaking that the Wellcome team did to balance the competing needs of different users.
A fascinating piece by Eleanor on the typographic tweaking that the Wellcome team did to balance the competing needs of different users.
Eric walks through a really nice use of CSS shapes and
@supports on a page of the An Event Apart site.
It's a nice little illustration of how we can use advanced features of CSS right now, without the usual wait for widespread support.
Story of my life:
I have to confess I had no idea what a technical leader really does. I figured it out, eventually.
Seriously, this resonates a lot with what I find myself doing at Clearleft these days.
I really, really like this approach. I've used something similar in my responsive design workshops, where I get people to break things down into nouns and verbs (objects and actions). I think there's a lot of crossover with good URL design here too—this is kind of like REST for UX designers.
This rousing call-to-arms by Abby the IA makes a great companion piece to her interview on The Big Web Show.
Well, this is rather lovely!
I nodded along with host Jen Simmons and guest Jeremy Keith saying some very smart things about the web and its roots as the El train cut across Philadelphia. But at the 48-minute mark things got weird, because Jen and Jeremy basically started writing my column for me while I listened.
Read on for some great advice on conquering your inner critic.
I'm so proud of Charlotte right now: last week she gave a conference talk and today she has an article published in A List Apart. Superb work on both fronts!
She does a great job of talking through a collaborative exercise to help teams move from thinking in pages to thinking in patterns.
Very thoughtful and sensible thinking from Paul.
Alla has taken the ideas she presented in her superb talk at Responsive Day Out and published them as a great article in A List Apart.
Every single word that Lyza has written here speaks to me so, so much.
I have no idea what I'm doing and I'm nervous about messing up, but I keep doing this week after week because it feels important.
Get out of my head, Lyza!
More thoughts on the lack of a performance culture, prompted by the existence of Facebook Instant:
In my experience, the biggest barrier to a high-performance web is this: the means of production are far removed from the means of delivery. It's hard to feel the performance impact of your decisions when you're sitting on a T3 line in front of a 30 inch monitor. And even if you test on real devices (as you should), you're probably doing it on a fast wifi network, not a spotty 3G connection. For most of us, even the ones I would describe as pro-performance, everything in the contemporary web design production pipeline works against the very focus required to keep the web fast.
Zeldman looks back at Stewart Butterfield's brilliant 5K contest. We need more of that kind of thinking today:
As one group of web makers embraces performance budgets and the eternal principles of progressive enhancement, while another (the majority) worships at the altar of bigger, fatter, slower, the 5K contest reminds us that a byte saved is a follower earned.
On the fifteenth anniversary of A Dao Of Web Design people who make websites share their thoughts.
Paul Ford's is a zinger:
I don't know if the issues raised in "A Dao of Web Design" can ever be resolved, which is why the article seems so prescient. After all, the Tao Te Ching is 2500 years old and we're still working out what it all means. What I do believe is that the web will remain the fastest path to experimenting with culture for people of any stripe. It will still be here, alive and kicking and deployed across billions of computing machines, in 2030, and people will still be using it to do weird, wholly unexpected things.
A superb piece by Ross Penman on the importance of being true to the spirit of the web.
A terrific bit of smart CSS thinking from Heydon Pickering.
You know he's speaking at Responsive Day Out, right?
Focus on what you want to learn; not what you think you should learn.
There is a lot of pressure out there: to learn new things, to spend all your time coding, to be the super developer. I now believe that to be impossible and unhealthy. It means you aren't living a balanced life and it also means that you're living under constant stress and pressure.
A great primer on using
srcset and
picture. I think I'll be referring back to this a lot.
I'm quite intrigued by the thinking behind this CSS selector of Heydon's.
* + * {
margin-top: 1.5em;
I should try it out and see how it feels.
Turns out that the :checked pseudo-class selector allows you to do some clever interaction without JavaScript.
A great article by Susan on getting started with creating a styleguide for any project.
I've seen firsthand how style guides save development time, make communication regarding your front end smoother, and keep both code and design consistent throughout the site.
Older »


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President Obama held a press conference today about how Congress just punched America in the face for no real reason (a.k.a., they let the sequester cuts happen) when Jessica Yellin of CNN asked Obama why he couldn't do something about this. Obama, trying to be clever, made this galactic gaffe:
"I can't do some kind of Jedi mind meld on congressional Republicans,"
Obama meant to say "Jedi mind trick." That's when a jedi uses the force to mess with your mind and convince you of something you otherwise wouldn't believe like "these aren't the droids you are looking for," or "it's bad to randomly punch America in the face."
The mind meld is a Star Trek thing. That's when two people voluntarily let their mental spaces seep into one another to achieve a greater understanding.
But wait there's more! The mistake actually describes a central dynamic of the Obama presidency: he came in wanting to do a mind meld (let's put aside the partisan games and get something done), but Republicans wouldn't play that game, so his only refuge is the mind trick (i.e. continually campaigning for his positions to put pressure on Republicans to do what he wants). Obama wanted cohesion, but he's left with coercion. He wanted the decades-old but reenergized by J.J. Abrams sci-fi franchise that preaches peace and reconciliation among a diversity of races and opinions, but he's stuck with the decades-old but reenergized by J.J. Abrams sci-fi franchise that has many of the same values but ultimately boils down to good vs. evil.
Sometimes it works (more liberal Violence Against Women Act passes the House, taxes on richest Americans went back up to Clinton levels), and sometimes America gets punched in the face.


text:
Fred Kelman first appears at the beginning of the film during the charity sky dive at Angel Grove Park. He tells his father to pay attention as this how the pros do it. His dad tells him that he didn't do half bad. Fred reminds him that he landed in the parking lot and it was embarrassing. He is a young boy who is friends with the rangers. He congratulated Tommy, on his sky dive and Tommy tells him that if he liked it maybe next time he should join them. He asked his father but he told him to wait another 10 or 15 years.
Fred was later seen in the park with a crowd of young kids where Ivan Ooze, disguised as a carnival wizard, is selling his jars of ooze. Fred buys a jar and takes it home. He leaves it at home, when he takes off again. When his father returns home from work he finds the jar and pulls some out placing him under Ivan Ooze's spell along with the other parents of Angel Grove. Fred later returns home trying to find his dad with no luck. He later ends up at a construction site where he finds his dad. He tries to talk to him but his father pays no attention to him. Fred tries to stay hidden as he watches hundreds of parents digging at the site.
He later follows Ivan and the other parents of Angel Grove to an abandoned factory where he overhears Ivan's instructions to the parents to return to the construction site and leap to their doom. Fred also finds out about the Ectomorphicon titan's that Ivan had the parents unearth. Fred races to Ernie's Juice Bar to enlist the help of the other kids. At first, he has trouble being heard, but he whistles loudly which gets their attention. He tells the kids that they have all been brainwashed and that Ivan is taking over the world using all of them in his evil plot and that he's got their parents. When a kid confusingly ask's Fred what he's talking about Fred reveals to them that Ivan Ooze is evil and he convinces them to come with him or their parents are going to die. The kids along with Bulk and Skull leave with Fred to try and save their parents. They reach the monorail where Ivan now as a titan destroys the track by stepping on it. When the kids noticed that the track is broken theWhite Ranger in his Ninja Falcon Zord arrives and places his zord in the broken track. Allowing the kids to proceed much to their excitment as they cheer.
When they arrive at the construction site, the kids are shocked to see that their parents are walking in a trance and speaking gibberish. Fred tells them to try to stop them and askes Bull and Skull to come with him. With their help Fred uses a bucket lift and an air hose to keep a steady pressure of water aimed at the parents just to try and wake them up. When the Power Rangers defeat Ivan Ooze, the parents are freed from the spell. Fred happily see's that his dad is back to normal and goes to embrace him. His father tells him that he loves him.
At the end of the film he would later join his friends at a celebration near Angel Grove Harbor. Fred was modest over his efforts to stop Ivan Ooze, although he did not mind the thought of being the Gold Ranger


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Belgian FN Browning Hi-Power Semi-Automatic Pistol....Click the image to load the highest resolution version.
DescriptionBelgian FN Browning Hi-Power Semi-Automatic Pistol.
Serial no. 53797, 9mm, 4 5/8-inch barrel with fixed front and rear sights. Blued finish. Belgium proofs to barrel, slide and frame. Checkered walnut grip panels.
Condition: Very good. Retaining approximately 96-98% finish with scattered handling wear and fine patches of pitting. Minor dings to grips. Action crisp. Bright bore.
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text:
Pear Mimosa
Be the first to review
READY IN: 50mins
Recipe by mollypaul
Jumex Pear Nectar is available in the Mexican ethnic foods section at the market.
See Discussion:
Ingredients Nutrition
- 2 (750 ml) bottles champagne, chilled (use ginger ale for a virgin drink)
- 4 cups pear nectar, chilled (Jumex or Del Valle)
- 1 lime, cut into 8 wedges
Directions See How It's Made
- For each serving, pour 3/4 cup of Champagne (or ginger ale) into a flute or wineglass and top with 1/2 cup of pear nectar.
- Squeeze and drop in 1 wedge of lime.


text:
Sociology: Exploring Human Society, is a college level introductory audio textbook for Sociology. This comprehensive audio textbook has 17 chapters covering the beginnings of sociology as an academic research discipline, culture and media, sociological research, socialization across the life course, social structure and social interaction, groups and organizations, deviance and crime, and social class and social stratification, global stratification, race and ethnicity, gender and sex, sexuality, family, religion, education and healthcare, politics, the economy, and population and society. Each chapter begins with an introduction that serves to guide the student and ends with a summary of the most important points.
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Warning: Activist Pseudo Science
I generally spend my time in psychology and neuroscience books. I've been wanting to start going after people-system topics for some time, so I thought sociology was a logical field to investigate. This book, an entry level text book, seemed like a good place to start.
It was. Sort of. The field and theories are interesting and thought provoking. But ...
What struck me was the great lengths the author went through to point out how sociology relies on the scientific method. Good. Then, everything seemed to be implicitly or explicitly couched in unsupported value judgements. Distracting to infuriating.
The author is at his worst as a scholar in the last chapters where he gives an irrational, overly simplistic summary (commentary) of the terrible state of the world, from our near complete destruction of the environment (I submit that in fact the planet isn't practically destroyed) to how the poor Katrina response was clearly racist (it may have been a contributing factor, but it certainly isn't the case closed explanation). The author even describes George Zimmerman's part in the Trsyvon Martin incident as having been panic because he (George) confused Trsyvon's skin color with a weapon. Really? This is your scholarly assessment of the situation? Shameful. This might be appropriate for a New York Times op-ed, but in my opinion, it has no place in a text book of a scientific field.
This author's approach is exactly why sociologists get little respect as scientists, which is too bad because we desperately need a better understanding of the workings of relationships among and within societies.
Bottom line: I recommend this book only if you are ok with a bunch of personal opinion and (very liberal) values mixed in with presentation of an introduction to a field of study.Rick
- Ricky D. Phipps
Needs a part 2
- Brendon Chikonyora


text: The original angry young man will be sharing screen space with the new one in the remake of Zanjeer. Bollywood superstar Amitabh Bachchan will be reportedly doing a cameo in the Ram Charan Teja starrer film, which will take off in April.The buzz is that director Apoorva Lakhia has written a scene, where the yesteryear and the latest Vijay will come face to face. Though the Bollywood superstar has not given a nod yet, it is very likely that he will be doing a guest appearance in the movie, as he is close to the Ram Charan Tejas family. In fact, unlike Don and Agneepath remakes, Zanjeer is being made after consulting and getting Amitabh Bachchans advice on this project. However, Ram Charan Teja is expected to meet the Bollywood actor soon to seek his blessings for the remake of cult classic.


text:
Finally a place to showcase all your custom projects that really don't fall into any other category. This forum is intended to share information amongst those that are making custom parts for their cars, as well as those with general welding/fabrication questions.
So let's see pics of your projects, whether it's downpipes, differentials, control arms, or fence gates!
Welcome to the Fabrication/Welding Forum
Started by Drax240z, Mar 06 2006 02:22 PM
2 replies to this topic
Posted 06 March 2006 - 02:22 PM
2000 BMW 528iT
1985 Chevrolet C20
2000 BMW 528iT
1985 Chevrolet C20
Posted 06 March 2006 - 06:16 PM
Finally, I'm really excited to see this forum open up.
Cody 82ZXT
12.9 @ 107.7
12.9 @ 107.7
Posted 08 December 2015 - 09:39 AM
This part of the forum has been especially useful in my rust repairs, thank you to whoever the person was that made this happen.
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text:
Climate change increasing freak weather
Climate change is increasing far faster than the world has been willing to admit, and New Zealand could face increased flooding and drought while large areas of coastline could be inundated by the rising sea and coastal erosion.
As another round of global climate talks began last week in Doha, a member of the generation that will face the effects of climate change, 18-year-old Ben Dowdle, believes those with the power to do something are not concerned about his future.
Dowdle was a Unicef youth ambassador at the climate change negotiations in Rio de Janeiro in June and witnessed the political inaction firsthand.
"It is definitely frustrating, we are the generation that have inherited the biggest issues of our time. Young people are getting more of a voice but politics doesn't think long term. It's why Rio+20 failed and it's why the talks in Qatar won't achieve anything," he said.
Fellow United Nations Children's Fund ambassador Rachael Hodge, 17, watched how people worked together when her home city of Christchurch was flattened by the 2010 and 2011 earthquakes.
Hodge hopes it won't take a climate change-induced natural disaster to make New Zealand aware of the effects of climate change.
"If we rely on a natural disaster to get people mobilised, everything is going to be a short-term response," she said.
Extreme weather events are going to become larger and more frequent in New Zealand and around the world as scientists begin to acknowledge that the more severe predictions of climate change are on course to become reality.
Last week the World Bank released a grave assessment of the potential effects of climate change.
A two-degree global temperature increase is generally regarded as dangerous climate change. The report, 'Turn Down the Heat', says that under current emissions levels the Earth will warm by an average of 4C by 2100, with dramatic consequences.
"To prevent the 2C rise is virtually impossible with our current rate of emissions. Of the climate models we use to predict warming, the ones that predict the higher levels are probably more accurate," said Professor Tim Naish, director of Victoria University's Antarctic Research Centre and lead author of the Intergovernmental Panel on Climate Change's fifth assessment report due next year.
The World Bank report forecasts droughts that will destroy agriculture, the flooding of coastal cities, unprecedented heatwaves and increased frequency and intensity of tropical cyclones, unless drastic action is taken to reduce the greenhouse gas emissions.
Dr Jim Yong Kim, the president of the World Bank, hopes the report will "shock" the world into action.
But policy makers have been surprised by the extent of the onset of climate change and now adaptation is taking priority over prevention, says Naish.
"The conversation has changed from mitigating, to how to cope living in a warmer world."
This year the world witnessed how difficult and expensive it is to cope with the effects of extreme weather.
There is no denying Hurricane Sandy was a monster of mother nature. But the influence of man-made climate change fuelled the storm, say many scientists.
The north Atlantic was unseasonably warm, sea surface temperatures were 3C higher than normal, (0.6C can be attributed to global warming) magnifying the storm by 5-10 per cent, according to New Zealand scientist, Dr Kevin Trenberth, senior scientist at the US National Centre for Atmospheric Research.
"The environment in which all these storms form is different than 20 to 30 years ago. Where they form is warmer and moister."
Which means more destruction.
"Ninety-five per cent of the time they look like storms we had before but they are really not. All of the storms that come through pick up those effects and can develop somewhat stronger."
As the water warms and sea levels rise, the increased frequency of extreme weather events is already being felt in New Zealand. Sea level rises will occur naturally, but New Zealand's low-lying areas will be vulnerable to the increased storm surges and rainfall of a warmer climate.
Last week Dunedin City Council responded to the effect by annoucing new homes would have to be built 1.2 metres higher off the ground.
"A 10-centimetre sea level rise increases the frequency of flooding in coastal regions by a factor of three. So a half-metre rise will increase the frequency of flooding by 300," said Naish.
"We are already getting to see that in Nelson where we are getting the one-in-100-year floods happening every few years now. That's not just because of sea level rise, that is because of extreme climate change."
If the climate continues to trend towards a 4°C increase, the West Coast is expected to get much wetter while the east of New Zealand will be much drier.
In the worst-case scenario, climate change will lead to highly drought-prone areas in the key eastern agricultural regions by 2050, according to a report on climate change and drought by the National Institute of Water and Atmospheric Research (Niwa).
And it is drought, not Hurricane Sandy, that will be the most destructive weather event to hit the US this year. Millions of hectares in the Midwest have been scorched by drought at an estimated cost to the economy of US$77 billion ($93.6b), the third costliest natural disaster in the country's history.
But the effects of 4 degrees of warming will not be evenly distributed around the world.
New Zealand's coastal climate and isolation mean it could be relatively well off. But if parts of Australia experience extreme drought and heatwaves, and the warming oceans fuel tropical cyclones and rising sea levels displace thousands of Pacific Islanders and South Asians, New Zealand will become a destination for climate migrants.
"There is growing recognition that climate change will exacerbate both the sudden and gradual environmental events and processes driving current patterns of migration and displacement," said Philippe Boncour and Bruce Burson in the paper Climate Change and Migration in the South Pacific.
New Zealand has already seen environmental migrants arrive from Australia after the Queensland floods and the Pacific archipelago Kiribati is looking to relocate its entire 103,000 population.
Hodge thinks Kiwis should be prepared.
"I hope that it is not just a lesson about being prepared for natural disasters it is about being prepared for a world that is always changing."
Sunday Star Times


text:
Cotton & Lacy Solid Patterned Bikni style Panty-Blue & Orange
Add to the innerwear collection this pretty and snazzy product from the house of Clovia. It has the following features:
• It is made from quality cotton and spandex material in pretty orange and blue colour. This material is super stretchable.
• The product bears a solid pattern.
• Comfort is guaranteed with cotton lining at the crotch which ensures easy movement of limbs.
• It has a soft elastic thick band at the waist. The same has a contrast orange colored lace trim at the leg opening adding appeal to the same.
• It has a medium waist fit.
• It is prepared in bikni style and provides medium coverage.Clovia Says Regular wear with class!
Material: Cotton, Spandex & Lace
Colour: Orange & Blue
Care: Handwash 30°C, Do not bleach, Do not iron, Drip dry in shade.
Comfort and style promised with this bikni style panty.


text:
Product Spotlight
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ampm's 'Edgy' Trade Show Puts Spotlight on Future
BP's convenience retail chief, Fiona MacLeod, pumps up crowd with talk of "pride, confidence, energy and excitement."
By Renee M. Covino
ANAHEIM, Calif. -- Was it a trade show or a Broadway show? The ampm national convention, held last week for the first time in a dozen years at the Disneyland Hotel, was a little bit of both—and had attendees looking towards a future filled with "too much good stuff" (its slogan, and also conference chant).
The retailer's first convention since going "all franchise" earlier this year drew more than 1,000 attendees (including about 80 percent of its franchisees), housed more than 130 exhibitors, and featured workshops, sessions and guest speakers, including outside retail experts such as Paco Underhill, consumer trends guru, and top BP parent-company executives, including Fiona MacLeod, president of convenience retail for the U.S.A. and Latin America.
But traditional show agenda aside, there was an unmistakable theatrical air about the place. The ampm brand was projected in a supersized neon-lighted sign, affixed to design-element scaffolding beside the conference stage. Meanwhile, dozens of inflatable, brightly-colored spheres that resembled planets, but with pointy ("edgy") spikes hung overhead, suspended from a blackened ceiling. And the chain piped in high-energy rock music, representative of the chain's three decades in business—the '80s, '90s and today.
The conference kicked off with "America's Got Talent" 2007 Finalist "Passing Zone," a male duo of comedic jugglers who finished their act in ballet costumes—and juggling powered-up chainsaws. Their moral to the audience: amazing things can happen when people trust each other and come together in teamwork.
"If c-stores were jugglers, our competitors would be juggling oranges and bowling pins, while ampm would be juggling chainsaws," said MacLeod in her vibrant Scottish accent, as she took the podium.
Her speech centered on having "pride in our past, confidence in our present, and energy and excitement about our future together." As proof of her own energy and excitement, she swayed with the groove of ampm's commercial music playing to the screen behind her—a video presentation of the 30-year-old chain's advertising history.
But the music and dancing hardly stopped there. Professional dancers took part in an elaborate "mini-musical" that highlighted in song and dance much of the ampm brand's new enhancements and improvements, including:
-- Its 24-hour hot-food offering, which includes the addition of corn dogs and a "bigger, better burger;"
--The pre-recorded hip music played at the conference will now be piped in to all stores, but with added "funky" ampm commercial messages; and
--The chain's just-unveiled new employee uniforms, which were designed with photographic images of the hot food and "too much good stuff" icons—corn dogs, burgers, big drinks, etc.—on its baseball caps, aprons and in a "strip" down one side of a royal blue uniform polo shirt.
"The competition is fierce, with other c-stores copying some of the best stuff we've got in ampm; but I'm here to tell you, ampm is fit for this fight and to win this war," said Don Strenk, vice president of operations. "We know customers don't come to us just for the corn dogs—it's the brand, the total offer, and how we execute it, and we're going to take it where no one has gone before—there will be a bigger, bolder ampm experience."
Strenk referenced an "operational excellence" program the company has in place for three years with its former company-operated stores; and now franchisees are coming on board with this practical approach to operations, which include best practices on how to clean the store, stock it, greet customers and other "best of the best" ideas from successful store managers and franchisees. Strenk told the crowd one franchisee who recently put the program in place saw a 62 percent sales increase in the prepared food area alone. Many stores have seen an overall 10 percent sales improvement "just by treating customers right" through the program steps, according to Strenk.
Overall, the ampm brand uses "humor, energy and authenticity" to attract and retain its customers, said Andrew J. Baird, vice president of marketing and merchandising, convenience retail. "Consumers are making significant shifts in their shopping behavior—they're reducing the number of stops they make—which makes it even more important that we give customers a reason to come back. We believe the answer is taking the ampm brand and turning it into a true customer experience that they can see, taste, hear and smell. We want to touch the senses of our customers so that they really know they are in an ampm store."
After the fanfare, in an exclusive interview with Convenience Store News, MacLeod and her convenience store team sat down to discuss the show and the future of ampm. Here are some of the highlights:
--On this being the first "national" show since 1996:
"We've always brought our top-performing franchisees together every year with a big incentive trip," said MacLeod. "But we haven't all been together; it's been in different groups. Now with all the new franchisees, we wanted to give everybody a chance to immerse in the culture and be connected. This is a great opportunity to indoctrinate them into the heritage and the history, and give them a chance to network with 30 years of experience out there so they can hit the ground running very, very quickly. And the fact that we're going national was just a great opportunity to take it to the next level."
--Explaining why the trade show was executed in such an "edgy" way:
"To me, ampm is a brand that wants to grow, and in order for us to grow we need passion, excitement and the commitment of our franchisees," said MacLeod. "And we don't want to stand still in the current environment; we want to grow through the current environment and get our people together and have them reconnect, have fun and rediscover their passion for ampm. From passion comes growth, it's genuinely how I feel about this brand."
"We don't want to stand still, we want to stand out," added Baird.
--A status report on converting company stores to franchise:
"We're actively recruiting in all our existing markets," stated Bill Fry, vice president of retail operations. "We've done Discovery Days in Cincinnati, Indianapolis, Orlando and Cleveland, and a few on the West Coast. We will be in 12 states total, once we've finished selling off the company stores."
"By the end of 2009, we would look to be entirely franchised with ampm," MacLeod stated. "My judgment as of now is that we're well ahead of that target—so we're very happy with where we are. In 2009 we're also planning to look at opportunities in other states. We have already had interest from jobbers looking to grow in states where we don't currently have an ampm franchise."
--On the consistency of the brand from store-to-store and state-to-state:
"If we're doing our job right, customers shouldn't notice much difference," MacLeod said. "About 85 percent of the stores should be consistent, with about 15 percent tailored to local markets, local demographics, maybe a higher weighting of Latino products, for instance. But the core products should be consistent. We want customers to walk in to any of our stores and not have to thinking differently about it."
--Regarding the recent ampm brand awareness:
"It has blown us away," maintained MacLeod. "The speed of customer recognition, customer relationship with the brand has just been so quick. Within days of stores opening, we see a whole different customer base coming in and shopping. In the market areas where we just launched the ampm brand, it's only been a matter of months, and it's already more than 50 percent, which is huge. And in certain key markets, where the brand has been there longer, the brand awareness is more than 60 percent. People just really 'get' the brand. They understand that ampm means 'morning and evening, I can get what I want.' They also know it's fun, they get that it's a c-store—it's a very real brand for people."