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Watch_Dogs Online is a 2015 Open World Third Person Shooter MMO that works as a prequel to Watch_Dogs 1. It has many key and new characters in this game. It has been given good reviews. There's also a levelling system that lets you do more powerful hacks.
Gameplay is still the same only the hackable objects are now varied. You run around in game,look for crime and do firefights with either a gang occupying an area of the city your in or police of that city. You can also drive vehicles. Your not Aiden Pearce in this title through but your controlling your own hacker with a pre-selected name. Also you don't take much damage from large or small falls.
There are 110 Characters in total in this game,not counting the Blume rising or Vegas takedown expansion packs. Heres a list of the key characters and minor ones.
- Aiden Pearce (NPC)
- T-Bone (NPC)
- Nicky (NPC)
- Jacks (NPC)
- Various Civillians
- Police
- Chicago Militia
- Gang Members
- Thugs
- Prostitutes
- Chicago South Club Members
- Jordi Chin
- Maurice Vega
There are many references to other videogames that are done by Ubisoft or done by another studio. You can find them scattered around Chicago as Easter eggs you can run into.
- Prince Of Persia:There is some graffiti on a wall in New York that shows the Prince jumping across a large gap.
- God Of War:You can find a copy of the first game in the God Of War series in Jacks Room
- Assassins Creed:Various characters when you profile them say Working for Abstergo the corporation that lets you relive your ancestors memories.
- Saints Row:The Corpse of The Boss can be found outside a Nightclub in New Orleans.
- Oddworld Dark Future: A copy of that game also appears in Jacks Room.
Reception so far from critics has been positive but were negative when the game launched. Many people heeded he criticism and stayed away from the game while the others enjoyed it and ignored the negativity of the critics.
(Trivia coming soon! Feel Free to edit and improve this page!)


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The Chandlery
The suites have 1, 2, 3 or 4 occupancy combo where you pay based on number in party, not room rate. The Chandlery is a large property with much exposed wood and spacious rooms. Park your car in the small park next to the house. All accommodation is en-suite, with flat screen TVs, free WiFi, central heating, radio, plenty of hanging space, refreshment trays. Breakfast from full English to (some say) the healthy option. You will be greeted warmly by a host who says he can talk on any subject and if you wish will inform you about footpaths, the unique local geology, fishing, pubs (nearest 300m down the road, next to garage with fuel and local shop), restaurants, towns and villages. There is much to see, from the incomparable Teme Valley to the ancient city of Worcester (15 minutes) with its magnificent Cathedral and the River Severn, Elgar s birthplace, the Malvern Hills and Witley Court. Card, cheque, cash, BACS. Check in and out times flexible, pets by arrangement. PLEASE all guests give a mobile number as contact, if available. Elgar Fam, grd flr, pte front dr & lounge, rear double & twin brms shower rm separate toilet/washbasin, desk & chair, luxury armchairs, chaise longue, 32 TV, antique pine floor/carpet through. Total Area 40sqm Malvern Fam, 1st flr, King 2s, fitted carpet. Light and airy, sunshine floods in. Desk, chair, 32 TV, Cape Dutch furnishing. Private, fitted bathroom, no shower. Total Area 30sqm Worcester Vaulted, beamed, rear located 1st floor room with plantation shutters to balcony overlooking attractive garden. Spacious tiled shower room, 32 TV. Total Area 21sqm Shelsley Fully carpeted, rear located, double 1st floor suite overlooking trees and horse paddock. 19 TV, fireplace. Total Area 14sqm
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subset: commoncrawl


text:
Doggy Day Care in Petersburg, OH
Drop off your dog at your sitter's home in the morning, and pick them up in the evening.
I work for Petco & have my own dog!
Jackson, OH, 45640
House Sitting, Drop-In Visits, Dog Walking
About: I work for petco and take care of animals on a daily basis. I have a 16 year old dog that I love and have taken care of since I was in 5th grade. I... (more)
Experience exceptional service and a personalized match
From start to finish, we'll handle all the details for you.Learn About Premier
Rover also offers doggy day care in:
|Beaver, OH
|Coalton, OH
|Egypt, OH
|Firebrick, OH
|Jackson, OH
|Minford, OH
|Pine Grove, OH
|Pinkerman, OH
|South Webster, OH
|Stockdale, OH
Don't let your 9-to-5 stop you from being a great pet parent. When you're heading to work, drop off your beloved pet with a loving dog sitter near you. Your dog will get the love and care they're used to at home, and you'll get photo updates and the peace of mind that comes from finding great care for your pet. We're proud to offer a wide range of pet care, including puppy day care and pet day care for all kinds of pets. We know you love your pets, but you can't always be there, which is where our loving sitters come in. Forget kennels, find a loving neighbor who will give your dog the one-on-one attention they deserve. That way, when you head to work, you know your dog's in good hands.


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My foster mom says that I am a proper lady and if given the chance, I like to show my nurturing side by grooming and caring for some of the other kitties in my foster home. Sometimes, I forgot that my human companion is not a kitty, so I also like to give her grooming, too!
My foster mom says that I am an equal opportunity lover of both other cats and humans. Sometimes, when I have had enough playtime and socializing, I just like to be alone. That's when I can get a little "catitude." Nonetheless, this passes and then it is time for my fun and grooming!
We hold adoption events on weekends between 12 noon and 3 p.m.. Please check our calendar for dates. Appointments can be made to visit the animals in the foster home.
Thank You!
Your message has been sent to Whiskers and Wags-4-Adoption.
You'll receive a copy, too, at to help you keep track of which pets you've inquired about, and which shelters and rescues you've emailed.
NOTE: Some shelters have physical locations you can visit; some of these shelters may only have pets for a limited time, so please do not wait for a reply—just go visit the shelter! Other organizations are rescue groups run by busy volunteers who may take a while to reply. You can find information about the shelter or rescue group caring for this pet, and their adoption procedures, on the pet's details page on Adopt-a-Pet.com.


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Doctor insights on:
Cbc Rdw Low
What does it mean when your CBC shows high RDW and low LY% and low LY#?
Probabky: a low grade infection. You are ok ...Read more
Complete Blood Count (Definition)
Share on twitter bookmark & share printer-friendly version a complete blood count (CBC) test measures the following: •the number of red blood cells (rbc count) •the number of white blood cells (wbc count) •the total amount of hemoglobin in the blood •the fraction of the blood composed of ...Read more
On a CBC what does it mean when it says MCH is 25.8 mchc is 31.6 both indicating low and RDW is 15.8 indicates a high read?
May not mean: A whole lot. These numbers could be seen in mild iron deficiency. ...Read more
My mchc is low at 30.6 my RDW is high at 16.6% rest of CBC is normal why is this ? I know I use to have low iron could this be it?
Not reliable test: Unless your hemoglobin is low(.Get a more detailed answer ›
Cbc results: low lymphocytes (15.7), high granulocytes 79.2, gr# 8.3, mchc low (32.4), MCV normal, RDW high (14.3), low mpv (7.7). Is this concerning?
Context: Without the proper context nothing can really be said about these results. ...Read moreSee 1 more doctor answer
Had another cbc. My RBC were 5.8. Up from 5.7 , hemoglobin 17.3. Up from 16.9. & hemotacrit 51.0 same as last time. Rdw 11.1. (low) is itpolycythemia?
Yes,i is polycythemi: Are you a smoker, are you living in a hilly area because these things can cause secondary polycythemia by less oxygen in the blood stimulating the bone marrow. But in addition to red cell count if white count and platelet count, the other 2 cellular types in the whole blood in addition to whole blood then it needs to be evaluated by a hematologist as it could be a condition called polycytemia vera. ...Read more
I got my CBC done.I have low hb, low total count, lowpvc, low mcv, low mch, low mchc, high rdw.What does it mean? Other parameters wbcand platelets normal.
Microcytic anemia: You need to have the cause determined. Even if it's common iron deficiency, you need to figure out why along with your physician. Don't accept anything short of a full explanation. Anemia is always serious. Best wishes for better health. ...Read more
I recently had a CBC test done and they found mcv-low, mch-low, rdw-high, and they found presence of nrbc's but i don't know how many. Reason for going in: headaches. What could it be?
NRBC: And the "indices" suggest a need for a heme consult. You didn't mention wbc, platelets or hematocrit/hemoglobin. Nrbc's are immature red cells, but it infers the bone marrow may be crowded out. Headaches doesn't help. The scenario can be serious. Leukemia and metastasis on the list, but you need moer information and I do too. ...Read moreSee 1 more doctor answer
Cbc done - hgb, hct low(10.6/33.3). The mcv, MCH and MCH were borderline low. The RDW high. Donated blood 1 month previous with 12.9 hgb. ? Diagnosis
Not enough data: Most cbc's only tell us what numerically is wrong. The only sure way to tell a CBC is abnormal is to look at the peripheral smear. Your indices indicate that you may be iron deficient, however that requires a thorough assessment for blood loss from the gi, gu and respiratory tract. If you donate blood, you can exacerbate the iron deficiency. ...Read more
5 yo cbc had slight low hgb/rbc. mcv and rdw is high w/slight anisocytosis. Referred to hematologist who said not urgent. Appt is in 6 wks. Worry???
No: Consider administering a pediatric vitamin with plenty of iron while you're waiting. ...Read more
My CBC shows low hemglobin of 10.5, mch, MCV and mchc low and RDW high
iron and tibc shows iron serum low 14 and iron saturation 5 alert
and ferritin se?
Iron def anemia: You have iron deficiency anemia-from your blood work provided. You need iron supplement-but most importantly to find out why you have iron deficiency anemia. Bleeding/blood loss- , malabsorption of iron in the stomach, or poor nutrition status/not enough intake of iron from the food could be the cause. Go to see your pmd and have more eval. If needed, referral to a hematologist can be done by pmd. ...Read more
Cbc (Definition)
CBC stands for complete blood count. This is a lab test that counts the different formed elements- red and white ...Read more


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Aunt Lynn's Best Ever Waffles
READY IN: 35mins
Recipe by Danlyn
I make these for my girls and my neices and nephews. I had one of them tell me these dont even need syrup.
Top Review by Cookie Puss
I am concerned there are ingredients missing. There is nothing to give the waffles lift. So I combined this recipe with one I already use to fill in the missing ingredients. The flavor was excellent, very sweet...great with fruit! But all the sugar made them stick to my waffle iron.
See Discussion:1 Review
Ingredients Nutrition
- 2 cups flour or 2 cups pancake flour
- 1 egg
- 1⁄2 cup sugar
- 1 tablespoon vanilla
- water or milk, to make soupy but not to thin
- 1⁄4 teaspoon cinnamon (optional) or 1⁄4 cup pecans (optional)
Directions See How It's Made
- in a medium size bowl add all ingredients except water/milk add that slowly.
- then using a belgan waffle iron make your waffles.


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International Panchkalyanak Mahotsav 11 Feb to 17 Feb 2016, Mahamastakabhishek from 18 Feb 2016
LIVE TELECAST of mata ji pravachan and update about Panchkalyanak Activities
ONLY ON PARAS CHANNEL EVERY DAY From 7:15 AM TO 8:05 AM
Shri Digamber Jain Atishaya Kshetra Khandargiri Place & Post – Chanderi, Dist. – Ashok Nagar (M.P.) Pin – 473446 Phone 07547 – 243118
Khandargiri is at a distance of just one km from historic city Chanderi of Dist. Guna situated in hilly area. Here are six caves in hills with many idols of Teerthankars. In Cave No. 2 a forty five high standing statue of Lord Adinath is very attractive and miraculous. These idols are carved by cutting rocks of hills, completed during 13th to 16th century. These hills are a part of the Vindhyachal Mountain range. This place is also famous for its religious training given to the jain disciples. It is not very much clear as to how old these caves and carvings are, and they could be much older than the general belief. The oldest dated cave is Cave 6, In this cave inscription in Sanskrit and Nagri script can still be found underneath each idol. An inscription dating back to 1236 A.D. can be read. Inscriptions telling of a Dharm Kirti 1645 A.D., Padam Kriti 1683 A.D., Lalit Kirti 1711 A.D.


text:
This article may contain outdated information that is inaccurate for the current version of the game. It was last updated for 1.7.
Districts allow the player to designate specific areas of the map in which you can assign specific policies and specialization.
The use of districts is not necessary, however industrial specialization can not be achieved without its use.
Creating districts[edit]
To create a district, click the Districts button located to the right of the zoning button. There, the player has 3 choices of brush size, and simply has to draw the district over the area they wish to cover by left clicking. The brush auto-snaps to roads and intersections, meaning that drawing individual blocks is relatively easy. To remove what has been drawn, simply right click and draw to erase. To expand the size of a district that has already been drawn, hover the brush inside the district until it is highlighted, then click and begin drawing.
District specialization[edit]
- Main article: Natural resources
Once a district has been drawn, it can be specialized. This is done by selecting the appropriate specialization from the bar at the bottom of the screen and clicking on the district. Specialisms control which types of industry develops in the district, and allows industry to fulfill production and secondary processing roles of the supply chain. There are advantages and disadvantages to each specialization.
There are 4 different industrial specialized district types:
- Forest industry - This district will cause any industry built inside it to work with timber.
- Agriculture - Agricultural industry consists of farmland and processors of agricultural goods.
- Ore industry - The ore industry mines and processes ore.
- Oil industry - The oil industry pumps and processes oil.
For a specialized zone to be a primary producer of a product, it needs to be zoned in an area with the appropriate natural resource. Otherwise all buildings in the area will be secondary processors of the specified type and will need to import the raw materials from elsewhere. Some secondary processing industry will normally emerge in areas of primary production as well.
Industry outside of specialized zones will be generic industry. Generic industry will not automatically utilize any natural resources present. A district's specialism can be removed, reverting it back to the generic type. Changing, adding or removing specializations causes all pre-existing industry in a district to be dismantled.
|Available only with the After Dark DLC enabled.
In After Dark, there are also 2 different commercial specialized district types:
- Tourist Commercial - This district makes money on entertaining tourists during the day.
- Leisure Commercial - This district entertains people during the night.
Finally you can remove these specialization using the third a last tool.
District policies[edit]
Districts can be subjected to differing sets of policies to the city as a whole.
Once a district has been made, specific policies can be assigned to it by clicking on the name of the district and clicking "policies" on the UI. This will open another UI to the right of the screen. The options will be mostly identical to the city-wide policy options, however these policies will apply only to the district.
Naming districts[edit]
On creation, a district will be given an automatically generated name. To rename a district, click on the name of the district, click on the text box of the UI that opens, type in the desired district name and hit enter.


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Do you employees miss their deadlines because they just don't have enough time in the day?
By knowing what's 'stealing' their time you can teach them how to manage it before their lack of productivity affects your company.
But, to help employees manage their time, you must first identify time stealers.
Do you know what the two common ones are?
Two time stealers and how to stop them in their tracks!
The Practical Guide to Human Resources Management, lists the top time stealers as…
Time stealer # 1: Communication overload - email and voicemail
Living in the information age has its real downside from a time management point of view. There's too much information and employers can be distracted by all the electronic tools available to them. As a result company productivity will suffer.
Solutions to solve communication overload
Time stealer # 2: Meeting overload
If your company has a culture of holding meetings, assess if your employees are constantly in meetings and as a result never get down to the work.
Four solutions to meeting overload
Knowing what's stealing your employees' time in your company will help you manage it so they can be more productive and meet their deadlines.
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text:
Red Nun Buoys
de Pollux
Acerca del libro
Why does Jon do all the talking? Because Auberon seems to be a good listener. But Jon is mostly having a conversation with himself, and looking for the hopeful things that buoy him in a sea of inner turmoil.
Características y detalles
- Categoría Cómic y novela gráfica
15×23 cm
20 Páginas
- Fecha de publicación mar. 24, 2013
- Etiquetas buoy, comics, friendship
Acerca del creador
Pollux (né Paul Morris) was born in 1979 in the English town of Beverley, in what was the former East Riding of Yorkshire. He and his family moved to the United States a year later, and Pollux attended UCLA, studying medieval history and Latin. He attended Brown University for grad school, studying medieval history. As a child, Pollux constantly drew and created his own comics, and while at grad school, he began drawing "Nigel and Beatrice" for The Brown Daily Herald. He has been creating comics ever since, creating the web comic Arnjuice. He drew the daily comic "The Wavy Rule" for Emily Gordon's Emdashes.com. His series of graphic novels, The Theresiad, features the surreal adventures of pharmacist Theresa Sifuer. Pollux received an A.S. in Graphic Design from the Art Institute of California in 2011. He also specializes in paintings and drawings depicting a wide variety of instrumentalists and musicians. His work has appeared at various shows in Los Angeles.
Inicia sesión o crea una cuenta para publicar un comentario.


text: Amisha Patel has been bitten by love. She has experienced broken relationships, and the latest been her breakup with long time boyfriend Vikram Bhatt. She has gone through hard times all because of love but she is still a complete romantic at heart. She said that love is like oxygen, which one should feel and breath. She added that life without love is as good as death. She admitted that it hurts when love fails but she still believes in it. She said that Vikram Bhatt is still one of her best of friends. She added that her relationships over the years have made her stronger.


text:
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Aqueous solutions of the detergent Fos-10 (n-decylphosphocholine) without and with addition of the integral membrane protein (IMP) OmpX (outer membrane protein X) have been characterized using pulsed field gradient-stimulated echo (PFG-STE) NMR experiments for measurements of translational diffusion coefficients. Effective diffusion coefficients for Fos-10 micelles in the absence of OmpX were obtained by observation of NMR signals from 10-bromodecan-1-ol that had been inserted into the micelles, and in the presence of OmpX by NMR observation of the protein. It is thus shown that solutions of Fos-10-reconstituted OmpX can be quantitatively described as a mixture of Fos-10 monomers, uniform Fos-10 micelles and uniform OmpX-containing Fos-10 micelles, with Fos-10 monomers in fast exchange between the pools of these three species. This result establishes an avenue for efficient determination of the effective translational diffusion coefficients of IMP-containing detergent micelles based on observation of the intense detergent NMR signals, which is also applicable with unlabeled IMPs. This monitoring of the species present in a given IMP solution contributes to improved guidelines for rational selection of detergent and buffer conditions in structural studies of integral membrane proteins.
Micelle-forming detergents are widely used to solubilize integral membrane proteins (IMPs) for structural studies either by X-ray crystallography or by nuclear magnetic resonance (NMR) spectroscopy in solution. Systematic studies of aqueous solutions of detergent-solubilized IMPs are therefore of practical interest. In previous work we observed that high-quality 2D [15N,1H]-TROSY-NMR correlation maps of uniformly 2H,15N-labeled OmpX (outer membrane protein X from E. coli) reconstituted in detergent micelles were obtained only over a very limited range of detergent concentrations.1 Investigations of the hydrodynamic properties of [2H,15N]-OmpX/Fos-10 micelles using TRACT2 and the recently developed TRO-STE NMR methods3 then revealed that the size of the OmpX-containing micelles was maintained at approximately 50 kDa over a wide range of detergent concentrations above (Na,pm[OmpX] + c.m.c.), where Na,pm is the aggregation number for OmpX-loaded Fos-10 micelles. This indicated that increase of the viscosity due to micelle formation by excess detergent caused the observed deterioration of the NMR spectral quality at high detergent concentrations. In this paper we further refine the characterization of aqueous Fos-10 solutions with and without addition of OmpX.
In solutions containing multiple pools of species with rapid exchange of detergent monomers between the different pools (Figure 1), the measured translational diffusion coefficients correspond to a weighted average of the contributions from the different species. To obtain the effective diffusion coefficient for Fos-10 micelles we added 10-bromodecan-1-ol as a "NMR probe" into the micelles, and for OmpX-containing micelles we obtained the effective diffusion coefficient from NMR observation of the protein. The detergent Fos-10 was selected because it has a large c.m.c., so that effects from the pool of monomeric detergent on measured apparent diffusion coefficients are expected to be readily observable. With a view to future adaptations of the presently described procedures with other IMPs and detergents, including work with IMPs at natural isotope abundance, the main interest was to establish a protocol for efficient measurement of effective diffusion coefficients based on observation of the intense detergent NMR signals. Another essential feature of the project is that all experiments are based on micro-scale protein reconstitution and the use of micro-coil NMR equipment, so that the individual experiments can be performed with micro-gram quantities of the IMP.
1μl of 10-bromodecan-1-ol was added to 60 μl of a 1.1 M Fos-10 stock solution in buffer A (5 mM sodium phosphate at pH 6.8, 10 mM NaCl, 90% H2O/10% D2O, 0.3 mM NaN3). This mixture was repeatedly stirred and heated to 50 °C until a clear solution was obtained. To prepare samples with Fos-10 concentrations in the range between 0.1 and 200 mM, the stock solution was diluted with buffer A.
[2H,15N]-labeled OmpX was expressed as inclusion bodies in Escherichia coli and purified to yield a solution of the unfolded protein in 6 M urea. NMR samples were obtained using a previously established microscale protocol that yields 1 mM solutions of [2H,15N]-labeled OmpX reconstituted in Fos-10 micelles in buffer A.4
All NMR experiments were recorded at 25 °C on a Bruker DRX-700 spectrometer equipped with a 1.7 mm TXI microprobe (Bruker, Billerica, MA). One-dimensional (1D) 1H NMR spectra were collected with the following parameters: data size = 16 K complex points, acquisition time = 1.38 s; number of scans = 128; sweep width = 11900 Hz. All NMR data were processed with the software TOPSPIN 1.3 (Bruker).
Translational diffusion coefficients for Fos-10 micelles at variable solution conditions were measured using the 1H PFG-STE NMR experiment.5,6 For each dataset a series of 16 diffusion-weighted 1D 1H PFG-STE spectra were recorded in a two-dimensional manner, using a pair of gradient pulses of duration δ = 4.5 ms that were separated by a delay of Δ = 50 ms, with gradient strengths, GD, ranging from 3 to 55 Gcm–1. Translational diffusion coefficients, Dt, for particles in solution were determined using the expression (1) for the signal attenuation, ΨQ, in PFG-STE NMR experiments,7
with Q = (γsGDδ)2, where γ is the proton gyromagnetic ratio and s describes the shape of the diffusion gradient, and Tdiff = Δ–δ / 3. All NMR spectra were recorded at 25 °C. GD was calibrated with the residual 1H signal in 99.9 % D2O by use of a self-diffusion coefficient for HDO at 25 °C of (1.902 ± 0.002) × 10−9 m2s−1.8
Assuming fast exchange of detergent monomers between the pools of Fos-10 monomers (m) and Fos-10 micelles (em) present at detergent concentrations above about 2 × c.m.c, i.e., τem/Tdiff 1, where τem is the mean lifetime of Fos-10 monomers in the micelles, we derived the expression (2) to describe the weighted average represented by the apparent diffusion constant, , measured by observation of Fos-10 NMR signals.9,10
where [Fos-10] is the total Fos-10 concentration in mM units with respect to monomers, c.m.c. the critical micelle concentration in mM, and Θ(x) the Heaviside step function with Θ(x) = 0 for x < 0 and Θ(x) = 1 for x ≥ 0.
Due to obstruction caused by the increasing density of solute particles in the solution, Dt,m and Dt,em are concentration-dependent, as given by Eqs.(3) and (4),11-13
and are the diffusion coefficients at infinite dilution of Fos-10 monomers and Fos-10 micelles, respectively, is the volume fraction of the micelles, and kem is the slope of plots of Dt versus the detergent concentration.12,14 is given by Eq. (5),14
where Vm = 0.494 nm3 is the volume of a Fos-10 monomer15 and NA is the Avogadro constant.
For spherical non-interacting particles, the Stokes–Einstein relationship describes the dependence of on the solution viscosity, η, and on the hydrodynamic radius of the particle of interest, Rh,
where kB is the Boltzmann constant and T the absolute temperature. To eliminate the dependence on η, we determined the "relative diffusivity" for Fos-10 micelles, dem, as3,16,17
where is a reference diffusion coefficient for a particle of known size at infinite dilution. The aggregation number, Na,em, for Fos-10 micelles can then be determined as
where Vref is the volume of the reference compound.
Decanol is insoluble in water but has a high affinity for binding to detergent micelles, and on this basis it was used as a 14C-labeled tracer molecule for the determination of the diffusion coefficient of micellar systems using the capillary method.18 Because the 1H NMR signals of decanol and the presently used detergent Fos-10 overlap, we used 10-bromodecan-1-ol as a NMR probe, since the bromomethylene group signal at 3.30 ppm does not show overlap with any of the Fos-10 resonance lines (Figure 2A).
The suitability of 10-bromodecan-1-ol as a specific probe for the study of Fos-10 micelles relies on its specific affinity for binding to the micelles and absence of binding to the detergent monomers. 1D 1H NMR spectra of Fos-10 micelles doped with 10-bromodecan-1-ol were used to determine the 10-bromodecan-1-ol: Fos-10 ratio, Crel, by comparing the signal intensities of the 10-bromodecan-1-ol bromomethylene group and the Fos-10 trimethylamino group (B and F in Figure 2A). After centrifugation to remove insoluble material, no NMR signal of 10-bromodecan-1-ol was detected in solutions containing Fos-10 concentrations below 10 mM, whereas for Fos-10 concentrations above 30 mM, Crel was approximately 6 % (Figure 2B). These results show that 10-bromodecan-1-ol binds exclusively at high detergent concentrations, where Fos-10 micelles are present. The 1H NMR signal of 10-bromodecan-1-ol can thus be used to measure the effective translational diffusion coefficient of Fos-10 micelles by 1H PFG-STE NMR experiments.
Considering that previous studies have demonstrated that experimentally determined c.m.c. values may vary depending on the sample conditions,19,20 it was of interest to determine the c.m.c. of Fos-10 directly in the solutions used for the NMR experiments. A basis for c.m.c. measurements is provided by Eq. (2), which shows for Fos-10 concentrations below the c.m.c. that the measured diffusion coefficients, , are equal to Dt,m, whereas for Fos-10 concentrations above the c.m.c., is the population-weighted average of Dt,m and Dt,em, and depends in a linear fashion on the reciprocal of the detergent concentration in the range where obstruction effects are small.21,22 Plots of versus the reciprocal of [Fos-10] above and below the c.m.c. can thus to a good approximation be described by straight lines, and the point of intersection of these two lines is the c.m.c.. The c.m.c. for Fos-10 under the NMR sample conditions described in the Materials and Methods section was thus found to be 16 mM (Figure 3). It is worth noting (Figure 3) that for Fos-10 concentrations close to the c.m.c., the experimental values are smaller than expected from the assumption that all Fos-10 molecules are either monomeric or part of uniformly sized micelles (see Materials and Methods). The deviation of the experimental values from those predicted on the basis of this assumption is indicative of the formation of pre-micelles.9,21,23 The data in Figure 3 could thus be rationalized by an extension of the scheme in Figure 1 for detergent concentrations at and near to the c.m.c.. Over an approximately four-fold range centered about the c.m.c., Fos-10 monomers would associate also into smaller aggregates than the micelles that are homogeneously present at higher detergent concentrations. These "pre-micelles" then grow to full-size micelles with further increasing detergent concentration, so that one has again a linear relationship between and [Fos-10]−1 at Fos-10 concentrations larger than about 30 mM.
Since 10-bromodecan-1-ol is only soluble in the presence of Fos-10 aggregates, the well-resolved bromomethylene 1H NMR signal of 10-bromodecan-1-ol can be used to determine Dt,em of Fos-10 micelles at Fos-10 concentrations above the c.m.c. (see Figure 3). Here we introduce the notation for the apparent diffusion coefficients measured with NMR observation of detergent signals at concentrations above the c.m.c. (see Eq. (2); a similar notation, , is used below for solutions of OmpX and Fos-10). Comparison of Dt,em with the corresponding values then demonstrates that rapid translational diffusion of the Fos-10 monomers makes a sizeable contribution to (Figure 4).
As predicted from Eq. (3), Dt,em depends linearly on the detergent concentration (Figure 3), and the parameters kem and were determined to be 2.9 ± 0.2 and 9.6 ± 0.1 × 10−11 m2/s, respectively. Since obeys the Stokes–Einstein equation, the aggregation number Na,em can be determined using an appropriate reference compound (Eq. (7)). We selected OmpX-containing Fos10 micelles as a reference, since their diffusion coefficient at infinite dilution, , and their volume, Vref, have previously been determined under identical sample conditions.1 The resulting aggregation number for the Fos-10 micelles of 55 ± 8 is close to literature values measured with different techniques, which are in the range between 45 and 53.15
In Table 1, parameters characterizing Fos-10 monomers, Fos-10 micelles and OmpX-containing Fos-10 micelles have been collected from the present work and from the literature.1,15 Based on this data, the apparent diffusion coefficients measured in aqueous solutions of Fos-10 and OmpX, , (Figure 3) have been analyzed for the situation at detergent concentrations above (Na,pm[OmpX] + c.m.c.) (Figure 1), assuming fast exchange of Fos-10 monomers among the three pools of particles:
The use of the fast-exchange approximation for was based on the observation that only a single set of narrow Fos-10 NMR signals was observed (Figure 2A), and is further justified in the Appendix. The total concentration of Fos-10, [Fos-10], was determined by 1D 1H NMR spectroscopy as described previously,1 [pm] was determined using the relation (10),
where the OmpX concentration was determined by UV spectroscopy1, and [em] was determined as ([Fos-10] - [pm] - [m]), where all the concentrations are calculated in Fos-10 monomers.
A comparison of experimental values measured in Fos-10 solutions with model calculations using [m] = 16 mM, which corresponds to the effective c.m.c. of Fos-10 micelles (Figure 3), shows close agreement (Figure 5A), thus validating the approach of Eq. (9) and the assumption of rapid exchange. In contrast, corresponding calculations with [m] = 16 mM systematically overestimated the diffusion coefficients for OmpX-containing Fos-10 micelles (Figure 5B). Variation of the input parameters within their experimental error ranges (Table 1) had a minimal impact on the resulting values. However, variation of [m] between 5 and 20 mM resulted in significant effects on the calculated diffusion coefficients, with the best agreement with the experimental data obtained for [m] = 10 mM (Figure 5B). The results presented in Figure 5 thus show that the presence of OmpX reduces the effective c.m.c. of Fos-10 by about 30%, from 16 mM to 10 mM.
The present investigation with the use of 1H PFG-STE NMR spectroscopy showed that a quantitative characterization of the composition of OmpX-containing Fos-10 solutions at detergent concentrations above (Na,pm[OmpX] + c.m.c.) can be obtained with the assumption that these solutions contain a mixture of Fos-10 monomers, uniform Fos-10 micelles and uniform OmpX-containing Fos-10 micelles, with monomeric detergent molecules in fast exchange between these three different pools (Figure 1). This finding expands on earlier observations1 that increased viscosity due to the formation of empty Fos-10 micelles in OmpX/Fos-10 solutions at high detergent concentrations causes deterioration of the protein NMR signals. Comparison of experimental and values with the results of model calculations based on Eq. (9) further showed that the presence of OmpX reduces the Fos-10 monomer concentration in the detergent sample from 16 mM to 10 mM, indicating that Fos-10 forms preferentially OmpX-containing micelles and that "empty" Fos-10 micelles are formed only by excess detergent, resulting in the aforementioned increased viscosity at high Fos-10 concentrations.
With regard to future practice of selecting detergents and solution conditions for structural studies of integral membrane proteins, these observations with the OmpX–Fos-10 system may contribute to developing more focused approaches: (i) There is now a rationale for the observation that high-quality IMP NMR spectra are obtained only over a narrow range of detergent concentrations: At one end, at Fos-10 concentrations higher than about Na,pm [IMP] there is increased viscosity arising from high population of empty detergent micelles, and at the other end, at detergent concentrations lower than what corresponds to the stoichiometry of the mixed micelles, the amount of detergent is too small to support a homogeneous population of OmpX-containing micelles. (ii) The effective c.m.c. for a given detergent can be significantly lowered by the presence of an IMP, which should be carefully considered when choosing "optimal" detergent concentrations. Although these considerations apply directly to obtaining high-quality IMP NMR spectra in solution, it seems likely that solution conditions similar to those optimized for NMR experiments would favor crystallization into well-ordered, diffracting crystals. It will be interesting to see to what extent the indications from the present work for the preparation of high-quality membrane protein solutions in structural biology will be generally applicable. To this end, corresponding data will need to be collected for other combinations of membrane proteins and detergents. It is to be hoped that guidelines will thus emerge which can be applied at least to sub-classes of membrane proteins, if not to integral membrane proteins at large.
We thank George J. Lu for help with the preparation of the Fos-10/10-bromodecan-1-ol samples. This work was supported by the Joint Center for Innovative Membrane Protein Technologies (JCIMPT; NIH roadmap initiative Grant P50GM073197 for technology development). K.W. is the Cecil H. and Ida M. Green Professor of Structural Biology at The Scripps Research Institute.
The NMR experiments of Figures 2 to to55 present evidence for rapid exchange of Fos-10 monomers between the pools of Fos-10 monomers, Fos-10 micelles and OmpX-containing Fos-10 micelles because only a single set of Fos-10 NMR signals is observed. Here, numerical simulations of different exchange regimes present additional, independent evidence that the Fos-10 exchange in the presently studied solutions is indeed very rapid.
To describe the impact of exchange on the signals in 1H PFG-STE NMR experiments, we applied the theoretical treatment of PFG-NMR experiments with heterogeneous systems by Kärger24 to the situation in OmpX solutions at detergent concentrations above (Na,pm[OmpX] + c.m.c.) (Figure 1). We denote the contribution from detergents in the ith site to the signal attenuation, ΨQ, as yi. In the absence of exchange, yi is given by Eq. (11),
where i runs over m, em and pm, and [i] represents the concentrations of the different species (Figure 1). ΨQ is then given by the sum of the individual components yi, which can be written as the scalar product of two vectors,
with e = (1, 1, 1) and y = (ym, yem, ypm). Eqs. (11) and (12) show that the signal attenuation in a non-exchanging mixture of monomeric detergent, detergent micelles and IMP-containing detergent micelles can be described by a tri-exponential decay if the three pools of species have identical detergent NMR chemical shifts. In the case of exchange between the three pools, Kärger has shown that the signal attenuation for Tdiff » δ can be described by a coupled linear differential equation,24
where the matrix LQ is given by
In Eq. (14), τm, τem and τpm are the mean lifetimes for a detergent monomer in the pools of monomers, detergent micelles and IMP-containing detergent micelles, respectively. According to Eq. (12), ΨQ is then given by,
where y0 =([m],[em],[pm])/[Fos-10]. In terms of the independent variable Q, the dependence of the signal attenuation on the detergent exchange is complex, since Eq. (15) does not represent a simple superposition of three exponentials. Although solutions of Eq. (15) can, in principle, be determined in closed form,24 we found it more convenient to perform the calculation numerically.
Comparison of experimental and simulated ΨQ data for a solution containing 1 mM OmpX and 110 mM Fos-10 shows that while a plot of the experimental ln(ΨQ) values versus Q is linear, all simulations for τpm values ≥ 10 ms show deviations from linearity. In contrast, the simulation with τpm = 1 ms matches well with the experiment, thus establishing an upper limit for the mean lifetime of Fos-10 molecules in Fos-10 micelles and OmpX-containing Fos-10 micelles of about 1 ms. Since the experimental data set was acquired at [OmpX] = 1 mM and a Fos-10 concentration of about 110 mM, the Fos-10 is present primarily in OmpX-containing micelles and as monomers ([m] = 10 mM; [pm] = Na.pm[OmpX] = 100 mM). Interestingly, satisfactory agreement with the experiment is achieved by involving exclusively fast exchange of monomers between the three pools of species in Figure 1, so that indirect pathways via collisions between the micellar species appear to make at most negligibly small contributions to the signal attenuation in PFG-NMR experiments.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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Perle Hoop Earrings
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These silver-tone hoop earrings feature a gold-plated ball-chain accent at the leather overlay. Lever-back clasp. 2.5" (6.5 cm) long.
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Latest News
Current Data Updated
Form41 Financials – New Q1 2017 Posted
T1 monthly traffic statistics available for September 2016
With our new tool, The Hub, comes a new name – Airline Data Inc. For more than 30 years, "Data Base Products" has been considered the Gold Standard for supplying quality U.S. commercial airline data. We are taking that solid history combined with the newest in leading technologies to bring a fresh look to the industry. Airline Data Inc, formerly "Data Base Products" – A new name with the same great data, same great history.
Watch for our new website which will launch in early September!
Launched in January of 2016, The Hub brings to bear industry-leading technologies that allow users to access all of their critical reporting needs within a few clicks. The Hub focuses on ease-of-use, providing clear and concise functionality. Our users drive our innovation, and The Hub prides itself on continual improvement and enhancements. When you log in, you always see what is new…with new functionality every month or so!
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Convenience Overseas
The Future of International Convenience Retailing to take place during September in London, featuring delegates from 20 countries.
LONDON -- With spring now sprung, sights are set to the summer and beyond including the next C-store innovation conference. The "Future of International Convenience Retailing" will take place in London from Sept. 15 to 18, 2008.
The conference will feature delegates from 20 countries who will join attendees in various progressive programs as well as studying two of the newest, most advanced c-store sites in the world, including the new Tesco Express and Somerfield.
Central London premium c-stores report some of the highest sales worldwide. The conference will, in part, provide a looking glass into the varied processes involved in attaining such success.
Speakers and presenters include Steve McDavid, Retail Marketing Director at SSP, Matthew Knowles from M&S, Jasper Wright, MD at Chop'd, Jerry Marwood, Managing Director at SPAR UK and Hamed Al-Naqeeb from Subway.
Additional speakers include: Gordon Campbell, MD at SPAR Intl.; Ed Burcher, senior director for foodservice with Petro-Canada; Karen Hubbard, UK convenience retail director for BP; and Mary Barnard, general manager, Quaker, Tropicana and PJ's Division Walkers Snacks. And an exclusive insight into SPAR UK's brand new multi-format Ostore and latest stores from retail director, Richard Bennett.
Senior convenience retailers and suppliers from the following 20 countries attended last year's event: UK, Ireland, USA, South Africa, Thailand, Denmark, Norway, Netherlands, France, Canada, Belgium, Czech Republic, Germany, Latvia, Romania, Argentina, Austria, Luxembourg and Iceland.
A 15 percent discount is currently being offered for early registration.
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Little Miracles Group
Little Miracles is a charity that supports families with children with special educational needs, disabilities and/or life limiting conditions.
We are hosting a coffee morning so we can start to successfully run a group which will help families interact and provide a safe non-judgmental, welcoming place for all. Families can come, relax, chat and most importantly ... Have Fun!
We would like you to come along and have an input so we know what you would like and expect from us.
Opening event Thursday 9th February – then every Thursday term time.
Rowans Family Centre, Moorfoot Drive, Fullers Slade, MK11 2BD
10.00 – 11.30am
For more information please contact Anne Cryer Whitehead on 07511782468, or email firstname.lastname@example.com. Places are limited invitation/referral only, please register for your FREE place.


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GREAT COOKS: Linda McCartney; Lots of Smidgens, But Hold the Meat
By BRYAN MILLER
Published: September 7, 1994
"ARE you a veggie?" is the usual salutation of Linda McCartney, cookbook author, photographer, musician and wife of Paul McCartney, the songwriter and performer.
Mrs. McCartney recently went to the East Hampton, L.I., home of the chef Pierre Franey to discuss her passion for vegetarian cooking and to prepare a meal from her cookbook, "Linda McCartney's Home Cooking" (Arcade Publishing, 1989). Traipsing behind her was her vegetable-loving husband, wearing baggy plaid shorts, sandals and a blue sweatshirt and carrying a guitar.
"I grew up in a family that loved good food, but I was the one who really liked to cook," she said while quickly getting down to business by chopping onions, carrots and leeks for a vegetable soup. "When I was growing up in Scarsdale, we had a cook. Maybe because of my insecurity or whatever I used to hang around the kitchen and watch her."
Mrs. McCartney, 52, disarmingly earthy and outgoing, has been married to the ex-Beatle for 25 years and has four children: Heather, 31 (from a previous marriage); Mary, 25; Stella, 23, and James, 17.
She was wearing blue jeans and a colorful peasant blouse -- "I'm a peasant cook," she said -- that set off her straw-colored hair and pellucid green eyes. Mr. McCartney, also 52, watched his wife buzz around the kitchen for an hour before quietly carrying his left-handed Martin acoustic guitar into the den for -- what else? -- a songwriting session.
"Don't mind me," he said. "I'll watch for a while, then go into another room to play a little." The McCartneys spend a few weeks each summer in East Hampton, near Mrs. McCartney's stepmother, Monique Eastman.
"Paul really is a great bread maker," Mrs. McCartney said while assembling her soup. "During the bread strike in England he made it every day at home."
Mr. McCartney said: "My best dish is mashed potatoes. A little milk, some chopped onions -- that's the Liverpool version."
Mrs. McCartney's devotion to meatless cooking goes beyond her household south of London. Three years ago she introduced in England a line of frozen vegetarian entrees: lasagna, pasta, burgers, pot pies, goulash and more. Today the line, which bears her name, carries 18 items and has more than $40 million in sales annually. Once the initial investment is recouped, she said, she plans to give the profits to charity.
In this country, Linda McCartney's Homestyle Cooking entrees, produced by Fairmont Foods outside Minneapolis, were test-marketed in the Midwest this year and are to be introduced on the West Coast next month and on the East Coast by year-end. Single-portion entrees will cost about $3 each.
For the meal at Mr. Franey's home, Mrs. McCartney prepared vegetable soup, an eggplant casserole laced with fresh herbs, coleslaw and applesauce cake.
"Most people don't know how good vegetarian food can be," she said. "Look at this vegetable soup. You know where the flavor comes from? Sauteing the vegetables in a little oil first."
The McCartneys, who raise sheep for wool and have an organic farm two hours south of London, in Berkshire, have been vegetarians for more than 20 years. An abhorrence of animal slaughter is at the root of the couple's aversion to meat.
"It really all started with the animals," Mr. McCartney said. "We were sitting home having dinner one day, and there were some lambs running around outside on our farm. They were so cute, and we were sitting there eating meat. Before that it never occurred to us that maybe we should look into another way."
He added that for the first year of their meatless lives, "there was this big hole in the middle of the plate, you know, because we were so used to meals built around meat."
"Then gradually," he continued, "Linda started filling that hole by cooking vegetables in interesting ways."
At the drop of a scallion Mrs. McCartney cites statistics on animal carnage and on the global economic efficiency of vegetarianism. And she never misses an opportunity to underscore her beliefs. When she spotted some ceramic figurines of pigs and cows on Mr. Franey's windowsill, she remarked, "Oh, those are cute, but they sure look different when they're murdered."
Mrs. McCartney refers to meat as "flesh" or when giving extra emphasis to a point, "a slab of fear." The McCartney children, all raised without meat, poultry or fish, have remained vegetarians.
"When the kids were growing up I said: 'Hey, we're going veggie. I don't want to cook meat anymore,' " she recalled. "If they wanted to eat differently out of the house, fine. But as it turned out, they were all sympathetic."
Even though all the children except James are now on their own, the McCartneys describe themselves as homebodies. "We don't get out that much, unless we're on tour or something," Mr. McCartney said.
"Yes, but the house is always full," Mrs. McCartney said. "The kids come by; we have friends over." At their East Hampton rental house the week before, they had entertained guests -- including Paul Simon, Lorne Michaels and his wife, Alice -- with a vegetarian dinner.
Mrs. McCartney noted that she was working on an updated vegetarian cookbook. Her current book has sold more than 250,000 copies worldwide, she said.


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Chicago Park Station plans
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- Hi all
Is seems that in the last year or two SOMEWHERE in one of the many
model railroad magazines out there I saw plans for the NCNG Chicago
Park Station. Now for the LIFE of me I am not finding it.
Can someone throw me a clue out there and tell me where the heck I saw
it at?
Frustrated in Texas
Mike D
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Today at the House Appropriations Committee markup of the Fiscal Year 2014 bill funding the US Department of Agriculture, Food and Drug Administration, and the Commodity Futures Trading Commission (CFTC), Congresswoman Rosa DeLauro (D-CT) continued to push for more funding and better policies on the issues important to millions of Americans. Unfortunately, the House Majority continued to block her efforts at every turn.
DeLauro's first proposal was an amendment that would eliminate a provision in the bill preventing American consumers from knowing the origin of the meat and poultry they buy in the grocery store by delaying the implementation of country of origin labeling rules.
"At a time when foreign companies are looking to purchase major US producers, our constituents should at least know where their food is coming from. Look no further than the recent announcement Shauanghui is trying to purchase Smithfield Foods. American food imports from China--and other countries--are growing, even as we struggle to ensure an adequate number of food inspections. American consumers need to be able to make informed choices in the marketplace."
DeLauro later offered an amendment to restore $60 million in funding to the Special Supplemental Nutrition Program for Women, Infants and Children (WIC). The money would support the critical work of breastfeeding peer counselors.
"WIC is a short-term intervention that can help provide a lifetime of good nutrition and health behaviors. Particularly in this environment, cutting funding for breastfeeding peer counseling is short-sighted, and will only result in higher health care costs. For a small cost, WIC pays enormous dividends in the health and well-being of our mothers and children."
Lastly, DeLauro proposed an amendment to restore funding for the CFTC to the President's request of $315 million.
"Americans want to see more accountability from Wall Street, not less. To make that happen we need enough cops on the beat at the CFTC, but right now the Commission is dangerously underfunded. Congress is effectively licensing more excessive risk-taking and bad behavior by banks and speculators. By not accepting my amendment, we have put in place a recipe for more reckless banking, more market failures, more bailouts and higher gas prices."
Source: http://delauro.house.gov/index.php?option=com_content&view=article&id=1310:delauro-continues-fight-for-american-values-at-appropriations-committee&catid=2:2012-press-releases&Itemid=21


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Alchester's relegation fears in Southern Counties North increased after they were edged out 22-20 at Drifters on Saturday.
Although the Fritwell-based club competed well, they are now five points adrift of safety, having won just three of their 13 matches.
Full back Alex Rankin ran in Drifters' opening try, but his knock-on led to wing Robin Tyreman scoring for Alchester, with fly half Tom Miles converting.
Two Matt Brown penalties made it 11-7 before Alchester scored a pushover try, with No 8 Joe McEneaney going over and Miles converting.
Brown's third penalty made it 14-14 at the break.
The same player added a drop goal, with Miles replying via a penalty.
Alchester had prop Dave Pike sin-binned before fellow front-rower Carl Corcoran went over for Drifters.
Miles slotted a second penalty, but Drifters held on.
On Saturday, Alchester travel to Wiltshire side Trowbridge in the semi-final of the Southern Counties Intermediate Cup.
Bicester are without a game in Berks, Bucks & Oxon Premier Division.


text: This web site presents information about Home Depot Bathroom Sinks with Cabinet. Bathroom cabinets were terms used to suggestion a easy white box that were typically mounted above the sinks in toilets. A little cupboard for one to keep make-up, tooth paste, tooth brush, your soaps, shaving razors, shaving lotions and a few medications. Those were the days taking into account toilets were basic and plain but now folks are making their toilets seem more refined and many are spending a lot of money a pain to make their toilet see and quality perfect. The bathroom cabinets used today generally in fact have a great deal of uses that are diverse practical and have become more in style and practical. This site provides a gallery of photos of the Home Depot Bathroom Sinks with Cabinet, supplementary bathroom Cabinet inventory. and provides help for design taking into account the counsel


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Let's take a look at how our crosstown rivals fared last night.
Before we get to the game, let's stop for a minute to discuss the Rangers this year. Their motto has been something along the lines of - "I feel the need...the need for speed!" - and it certainly has been entertaining to watch. Leading up to every game, we hear the players stating some variation of how they need to use their speed. The NHL betting lines have shifted as a result and the Rangers are often favored most nights.
But what happens when an opponent is able to slow that speed game down? The Senators gave us the answer last night.
People have stated how great Anderson was and also complained how frustrating Boucher's system is to compete against. While it's true that Anderson was fine he easily attained the shutout since he was rarely challenged. It's true that the Senators' defensive style is boring to watch. However, good teams will work around those things. Good teams come to the came with more than just Plan A.
Personally, I defend Vigneault. However, his inability to make in-game adjustments is beyond maddening. If the Rangers want to play a speed game, that's great. However, if an opponent is filling up the neutral zone, then you need to adjust. For Vigneault, he continues to try and put a square peg into a round hole. This stubborness is what ultimately doomed the Rangers last night.
The Rangers tried over and over again to skate through the wall Ottawa on the red line. They continued to turn the puck over and the Senators capitalized. It's one thing to be stubborn and keep at the plan through the first period. Why the team didn't alter the game plan during the first intermission is mind-boggling.
Halfway through the third period the Rangers finally shifted to a dump and chase game. However, the Rangers forgot that they have to "chase" after they "dump". This strategy at least got the puck got near Anderson. Their shots were limited to the perimeter but it was a start. Perhaps if they changed to this strategy during the first or second period, they may have ended up with a goal or at least challenged Anderson.
The defense took a lot of the blame and certainly they did not have a great night. However, they did get the pucks out of their zone, only to see the forwards turn it right back over. The Rangers defense is certainly not perfect but when the team has a bad defensive outing, I think the blame needs to be shared with more than just those six player. The forwards are guilty as well.
It's interesting to think about what the Rangers goalies feel during games like this. Late in the third period, the Senators appeared to be on a breakaway. Raanta headed towards the blue line. He was able to stop the play but then sent the puck up the ice to give the Rangers a scoring opportunity. This was the best pass of the night. It appeared that Raanta would have skated it down himself. If the goalies can see the problem, then, I don't understand why the coaches and players do not. If they can fix that, then Vigneault might actually get the Jack Adams this year. And to top it off, the Rangers probably would have won last night.]]>
The New York Rangers were active over the offseason, acquiring talent and getting healthy.
Now the Rangers enter the preseason looking to make a run and stand as one of the more intriguing teams bettors should consider when playing NHL futures and lines. With so much potential for a solid payout, bettors should take a look at NHL online sportsbook odds and consider making a wager given the positivity around the franchise.
Here's a look at the latest preseason news and notes around the Rangers.
Maxim Lapierre Gets a Tryout
The Rangers will have center Maxim Lapierre in camp for a tryout before the season. He's a veteran of the MODO Hockey in the SHL and HC Lugano of the Swiss National League A. Now 31 years old, Lapierre was last seen in the NHL in 2014 when he suited up for 80 games with the Pittsburgh Penguins.
There, he recorded 11 points and served in a smooth rotational role. The Rangers could use such a presence, provided Lapierre nails his tryout.
Mackenzie Skapski Back on Track
The Rangers were on the receiving end of a major blow last year when Mackenzie Skapski suffered a hip injury and didn't have a chance to suit up.
A high-upside goalie at the age of 22, Skapski showed promise in 2014 and has throughout his developmental trek. Word out of New York's recent developmental camp is he looks back to 100 percent after surgery, which is huge news for both Skapski and the team. A fully healthy Skapski means the Rangers have more options than usual this upcoming season should the coaching staff need to promote someone to the main roster.
Mika Zibanejad Looks Ready
Mika Zibanejad is another example of what a solid job the Rangers have done building up their system over the years.
Zibanejad is just 23 years old and just landed with the Rangers via a trade with the Ottawa Senators. Last year he trumpeted this upside with a strong 21 goals and 51 points, with word out of camp this year suggesting he's ready to post even more production. This would obviously be a huge boon for the Rangers, who coughed up elite scoring option Derick Brassard in the trade.]]>
The Wild also had some welcome days to rest and recover from what has been a physically demanding last few games. With some bumps and bruises that have kept Zach Parise, Marco Scandella and Erik Haula out of the lineup, the Wild have been forced to adjust but so far have shown resiliency which was an issue during the previous coaching regime. Another struggle the previous regime seemed to have was its struggles to get back into form after a few days off. Can this staff keep Minnesota focused and executing at a high level against what should be a motivated Sabres squad hoping to avenge a loss to the Wild from a week ago? For those of you interested in online hockey betting, this certainly is an interesting question.
1st Period Thoughts: Someone grab the small bottle of 5 Hour Energy, a bottle of Jolt soda or some Tabasco Sauce and have each member of the Minnesota Wild drink all of it. Because after a catatonic period where the Sabres outhustled, outplayed and outchanced the Wild it makes you wonder if they were asleep. It was lethargic from the Wild right from the drop of the puck. Buffalo was winning all of the little races to the puck and they were not hesitating to work pucks to the point men who then would rifle shots on goal and then look for rebound. Devan Dubnyk found himself under siege early and a just a few minutes in a shot from the point by Rasmus Ristolainen would hammer a slap shot that was stopped by Dubnyk but he was unable to stop Ryan O'Reilly's rebound bid. 1-0 Sabres. Minnesota didn't seem to be awaken almost at all after Buffalo's early tally and they continued to mostly sleepwalk through the rest of the period. Lazy area passes with lots of standing and reaching instead of skating meant the Wild were going to continue to have chase the Sabres around its offensive zone. The lines that looked so good a few days ago now looked disjointed, mostly uninspired and ineffective. It seemed that on any given shift, you'd have one Wild forward that would hustle and the rest would just sort of watch and hope he was able to get the puck somehow. Even as Dubnyk denied Nick Baptiste, the team continued to sort of go through the motions. One player who I felt was exceptionally bad was Mathew Dumba. The normally offensive-minded defenseman completely spaced and didn't hold the zone and then as the Sabres counter attacked he half-heartedly defended and the result was Buffalo nearly added to its lead. Few Wild players seemed interested in creating much offensively and the result was we did very little to test Robin Lehner. I know Bruce Boudreau complained about the lack of players available to practice due to salary cap constraints, but that doesn't excuse this team being outworked and outhustled after having two days to rest up prior to tonight's game. Minnesota would be wise to copy Buffalo's approach and keep it simple and work for a goal and put some pressure on the younger and less experienced Sabres.
2nd Period Thoughts: The 2nd period the Wild would awaken a bit, but poor execution nearly turned it into a disaster. Minnesota started to move its feet more and this meant they did not spend the period chasing around its own end of the ice. Even though the hustle was there, the decision-making left a lot to be desired. A few cases in point. 3 on 1 for the Nino Niederreiter, Charlie Coyle, Eric Staal line (which was by far the best line of the game so far for Minnesota) but with Nino with the puck and a clear look at the net tries to pass the puck to Coyle who seemed surprised and they didn't even get a shot off. A few minutes later it was the Wild catching the Sabres on a poor line change and without too much trouble had a 4-on-2. Instead of pressing the attack they slowed down and no one seemed to know what to do and by the time the puck worked its way to Dumba he had no clear shooting lane and another odd-man rush would go without a shot being taken. You could feel the frustration in the crowd and Minnesota tried to clean up its act and the next time the Niederreiter-Coyle-Staal line got a 3-on-2, Nino pulled the trigger and Lehner had a tough time fighting off the shot. A power play would finally give the Wild the chance it needed to bury the biscuit as Jason Pominville made a nice play to work the puck deep in the offensive zone, sending a pass to Staal who let go a low-lying shot that yielded a big rebound that was swept by Mikael Granlund who moved in dangled around a sprawling Lehner to backhand it into the open net. 1-1 game and Minnesota looked to have finally awaken. Minnesota out shot the Sabres 15-7 in the 2nd. Another player with a good period was Tyler Graovac who was moving well and making good plays at both ends of the ice. I like how he was taking the puck to the net and throwing his 6'5" frame around.
3rd Period Thoughts: The 3rd period would see the Wild ease up and the Sabres waited patiently to ramp up the intensity and Minnesota deserved the fate it received. Minnesota seemed to want to work for some pretty finish and more often than not this resulted in no shot at all. The Sabres would wait for Minnesota to make a mistake and it would come about halfway through the period when the team caught the Wild pinching with its worst defenseman, Nate Prosser and it was Brian Gionta racing into the Wild zone and swinging a centering pass that partially deflected by Jared Spurgeon and onto the stick of a crashing Johan Larsson who tapped it by Dubnyk. 2-1 Sabres and you'd expect the home team to be fired up after that small defensive breakdown right? Nope, it was more of a lethargic effort while Buffalo seemed eager to add to its lead with simple, in-your face hockey. Buffalo nearly added another when Ennis set up Marcus Foligno for a tap in chance after he outhustled Jonas Brodin for a loose puck. Only a Dubnyk save kept the Wild within one. Minnesota continued to look uninspired and it wasn't until the final two minutes, leading up to the Wild pulling Dubnyk for an extra attacker that the team started to show some urgency. Yet it was too little too late, as the Sabres continued to force the puck to the wall and just outworked us and Minnesota didn't even get a shot on goal with the extra attacker and the Wild fell 2-1.
Devan Dubnyk played well enough for the Wild to have a chance in this game, making 22 saves in the loss. He didn't have to face many chances tonight, but I thought he was left out to dry a bit by his defenseman whose effort seemed to be a bit lax this evening. Even Christian Folin who was coming off some of his best hockey was soft on the puck at the worst time when O'Reilly got the opening goal of the game. Same was true on the game winning tally as Spurgeon opted for an interception instead of taking the body against Gionta, not to mention a lazy effort to get back in the play by Prosser who should feel awful after his ill-advised pinch. Zero excuse for lazy, leaning and reaching play the Wild's defense had tonight after having the last two days off.
Offensively the Wild had one line that was going in the Staal-Coyle-Niederreiter line but even that group wasn't generating enough shots and quality chances as they were guilty of trying to make the perfect play instead of a simple one. The rest of the lines had a few flashes but were mostly forgettable or lacked cohesion. The 4th line of Graovac, Schroeder, Bertschy didn't add much of anything and the Sabres often would try to press the attack when they were on the ice. A few bright spots were the play of Staal and the pesky play of Pominville who I feel has been pretty understated while being bounced around to just about every line the Wild have.
This was a game where the Sabres nearly left the Wild off the hook for its poor effort, but Minnesota just couldn't capitalize on the chances they were given. From the failed odd-man rushes to simple let downs defensively are what ultimately cost the Wild at a good chance at another 2 points in the standings. Yes this team is beat up, but it still does not excuse being outworked as much as they were tonight by the Sabres who simply seemed to want this one more than the Wild did. Now they have another few days off before they go and play what will likely be a very motivated Colorado squad. The question is, will the Wild be motivated or will they look like they did this evening?
Wild Notes:
~ The Wild roster was as follows: Mikko Koivu, Mikael Granlund, Joel Eriksson Ek, Eric Staal, Charlie Coyle, Nino Niederreiter, Jason Pominville, Chris Stewart, Tyler Graovac, Jordan Schroeder, Christoph Bertschy, Ryan Suter, Jared Spurgeon, Jonas Brodin, Christian Folin, Nate Prosser and Mathew Dumba. Darcy Kuemper backed up Devan Dubnyk. Erik Haula and Marco Scandella are the scratches.
~ The 3 Stars of the Game were: 1st Star Ryan O'Reilly, 2nd Star Mikael Granlund, 3rd Star Johan Larsson
~ Attendance was 18,864 at Xcel Energy Center.
Iowa Wild Report:
Iowa 3, Milwaukee 2
Iowa would get out to a fast start as Alex Tuch set up Colton Beck for a quick strike just a little past 3 minutes into the game. The Admirals tried to answer back but Steve Michalek was rock solid, weathering the storm and keeping Iowa holding onto a 1-goal lead. Iowa would add to its lead less than a minute into the 2nd as Jordan Schroeder was just able to tuck in a shot near the right post to make it 2-0 Wild. The Admirals would answer back with two goals from Matt White and a power play tally by Adam Payerl from in close to tie the game at 2-2. A few minutes later it was the Wild who struck after a frantic few seconds near the Admirals crease that had Juuse Saros flopping around but unable to cover the puck up before Sam Anas was able to jam the puck over the goal line, 3-2 Wild. Iowa would then lock it down in the 3rd and the Wild would finish the home-at-home with a 2-game sweep. Michalek was superb making 36 saves in the victory.
Wild Prospect Report:
LW - Kirill Kaprizov (Salavat Yulaev Ufa, KHL) ~ the talented winger is certainly enjoying the fact he's playing for a much stronger team, as he chipped in a primary assist in Salavat's 6-0 win over Sibir Novosibirsk. Kaprizov has 10 goals, 20 points, 62 PIM's and is a +9 in 24 games.]]>


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Battlefield Heroes is one of those titles that easily crosses a few genres. There's no question, however, that the game has a good chunk of free-to-play MMO shoved within its most gooiest bits.Just for history's sake, this will most likely be the third Battlefield game I will play. What's funny about that is the fact that I skip every other one. I played Battlefield 1942, skipped Battlefield Vietnam, played Battlefield 2, skipped Battlefield 2142, and will probably be playing Battlefield Heroes.
Eurogamer has the news that sign-ups will be handled over on the official website on May 6th for anyone interested in playing this quirky-go-lucky online shooter. If you somehow haven't seen this inspiring trailer, you really need to check it out. The character screen features plenty of hard-points for character clothing options and the required level/experience-to-next-level indicator that completes the MMO addiction trifecta.
It goes without saying that we'll be tossing our hats into the beta ring. The gameplay looks exactly how you would imagine a WWII-cartoon-styled persistent online first person shooter -- or WW2CSPOFPS if you love acronyms -- would look like; strangely awesome.
Star Wars: Rogue One Review - Since seeing Rogue One (twice now) I've been thinking about Star Wars Galaxies (SWG), the ill-fated Star Wars MMO. A key moment in my history with SWG was...
7 months ago


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About MeI live on the west coast and travel a lot to shoot. I do about half fashion and about half action sports (primarily wakeboarding and snowboarding) I love and have a passion for shooting them equally.
I like working with designers and have shot for some great upcoming designers such as German Madrigal and Sam Duncan.
I'm definitely "A Creative" and enjoy coming up with new ideas, new places to shoot and interesting concepts. I rarely shoot in a studio and like to work with models who are creative and energetic on location. If you have a shot or project you've been wanting, let me know, I am always willing to work on new and inventive ideas.
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UK Small Business Directory
Reference Number. 52200
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Millennium89c grange road
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Millennium Contractors has built its success and reputation on high levels of customer care and workmanship. We Supply registered and qualified contractors to private domestic customers and commercial clients All contractors are registered Sub-Contractors, they all are fully qualified and have at least 6 years experience within the trade, thus you can be assured quality work along with a professional service. We are hired by individual registered contractors to carry out a number of their duties such as customer liaison, booking in job(s), admin and many more. We do not take any commission or payments for any work, all jobs are priced at a fixed price by your allocated engineer, we simply take down your details allocate a registered contractor to your job, send you all your paperwork and simply make life as easy as possible for you the customer and the contractor. We offer Corgi Registered plumbing and heating contractors whom individually hold 6 years plus experience and are fully qualified within their regions of work. We are dedicated to providing an efficient and quality service to all our clients and domestic customers. Our contractors pride themselves in the quality of workmanship and guarantee that all work undertaken by our contractors will be carried out to the highest of standards. We have a full compliment of CORGI registered contractors that includes not only plumbers but tillers, electricians and builders (for more information regarding contractors other than plumbers please speak to a member of our team). We pride ourselves in offering the highest quality of workmanship and service. Whether the work is basic repairs, new installation or a full bathroom renovation, we ensure every customer is satisfied to the highest of standards. Our contractors are based in the London, Greater London and Essex areas, thus you can rest assure we can provide a qualified contractor within you local area. Current Offers Boiler Swaps - Com...
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