text:
I MUST BE getting soft in the head. That, or a predominance of national opinion-makers have grown so cynical and callous--and so thoroughly co-opted by the bottom-line mentality that equates good and bad with plus and minus signs preceded by the $ symbol--that intangibles such as citizenship, leadership and cooperation in pursuit of public good no longer have meaning in America.
Now if you wise-asses are through chortling over the freebie I dished-up at the top of this column, let's move on to the meat of today's missive:
The state of the union.
In a word: sorry.
In another word: money. It seems the boys and girls in Washington and in most of the penthouse offices in most of the state capitols of America are fixated on the almighty dollar and damn little else. I watched Bill Clinton's state of the union address last week, and found myself moved by what the man had to say. I don't know if it's entirely a function of my advancing years, but my heartstrings hum to the tune of anthems and spirituals more readily now than when I was younger and knew so much more. It cheered me to hear the President speak of the need to put party politics aside and keep our minds on what's best for the country. I enjoyed and applauded the simple sensibility of his observation that it ought not have to take a flood or an earthquake or a tornado for us to act like Americans who believe in the American credo of equality. I laughed when Clinton told about the lady in California who did "what few of you in this room would do. She hugged me and said, 'I'm a Republican, but I'm glad you're here.' "
You know what? That kind of honest, self-deprecating humor is a precious thing to have in a nation's president. Bill Clinton may not have as great a store of other virtues as some might prefer, but he has humor, and he has a gift for stirring speechifying that in less-cynical, less-materialistic times could itself mobilize a nation to achieve great things.
But we no longer seem capable of believing in anything unless it is wedded to our own financial gain. Consider: The last Republican President we had was George Bush. What did George stand for? Thinking real hard I can summon three things from memory: A thousand points of light; a kinder, gentler nation; and restoration of large tax deductions for capital gains. The first two were literary conceits from the imagination of speech writer Peggy Noonan and amounted to nothing, even as symbols. The third was the lodestone of Bush's political philosophy--let's help the rich get richer--but the man was so uninspiring he couldn't even get this piece of self-interest through Congress.
Ronald Reagan was reckoned to be quite the inspirational leader, long on symbolism and feel-good, but what did he truly symbolize? Again, the answer is greater wealth for the already wealthy. Reagan didn't accomplish this by cutting taxes; he did it by deregulating commerce. The robber barons made out like the bandits they are, and the national debt tripled. The gap between the haves and the have-nots in America grew, by every measurable standard.
You've got to go clear back to the inauguration of John Kennedy to recall the last time an American President uttered a call to altruistic public service and was taken seriously. He asked us to ask not for ourselves, and some of us took it to heart.
If John Kennedy had been standing where Bill Clinton stood a week ago last Tuesday, and said what he said 35 years ago, the instant analysis from the networks would have laughed him off the podium.
Clinton tried, bless his heart, and it stirred something deep in my soul--something I enjoy still finding there. He spoke of nationhood instead of party politics. He spoke of compassion and humanity instead of personal gain. He spoke of the need for keeping families together, of protecting them from ruinous medical costs, of a minimum wage somewhat nearer to a living wage...
...and they laughed at him. Not while he spoke, but afterwards, in almost pitying tones. These pipe-dreams will never fly, they said--not just the Republicans, but the press.
And then Christy Whitman, the newly elected governor of New Jersey, delivered the official Republican Party response to Clinton's address, and what did she say?
Cut taxes. Cut taxes, Cut taxes. Oh, and did I mention cutting taxes?
This is leadership? This is what passes today for public service? Any idiot can cut taxes--it requires no genius nor imagination, no serviceable ideas for coping with the complexities of administering to the needs of 250 million people in a perilous world. You just cut taxes, which put a few hundred bucks a year in the pockets of the vast, middle-class majority, nothing in the hands of the poor, and thousands to millions in the bank accounts of the rich.
And the class war looms nearer.
What the hell has gone wrong with this nation? Is it so preposterous a notion to assure the working poor of $5 an hour? The new Republican majority in Congress sees no problem cutting taxes, which costs revenue and benefits the rich more than the poor. They say tax cuts stimulate the economy. Well, a fairer wage for the working poor benefits the economy, too. They've got to spend their raises--it's not enough to squirrel away in savings. Any increases in the minimum wage go right back into the economy, at the grocery check-out line and into the rent check.
I can understand, in all its perversity, the Republican fixation with punishing people on welfare. What I cannot square in my own heart and mind, nor with the Republican myth of hard work and self-reliance, is refusing to let the foundation of the American work force--those who are doing the dirty work, and are at greatest risk of sliding backward into unemployment, the public dole or crime--a fighting chance to work their way out of poverty and into independence.
At present I'm forced to conclude we are a nation of selfish hypocrites. At least judging by the government we have elected, this is the only logical conclusion. It won't stay this way forever.
I only hope I live long enough to see it get better rather than worse.
| © 1995-97 Tucson Weekly . Info Booth


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A love story in the digital age. A girl and a boy together for a moment. What if the photographs they take were to disappear? Memories are our most valuable property, but we save them on very fragile media. Hard disks are particularly vulnerable and break easily so you can lose everything in one go.
IMDb Link: http://www.imdb.com/title/tt2417540/
Facebook Link: https://www.facebook.com/36MaryIsHappy (please like!)
Genre: Drama
Distributor: Day For Night
Year: 2012
Country: Thailand
Director: Nawapol Thamrongrattanarit
DVD Source: DVD5
DVD Format: PAL
DVD Size: DVD5
Programs Used: DVDFab
Video Bitrate: 4935kbps
Screen Format: 16:9
Audio Language: Thai
Audio Format: 5.1 (AC3)
Subtitles: English
Menu: Untouched
Video: Untouched
DVD Extras: Untouched
Uploader's Comments:
36 (2012)
Written and Directed by:
Nawapol Thamrongrattanarit
Vajrasthira Koramit
Wanlop Rungkamjad
Cinemanila International Film Festival 2012
Won: Best Director International Competition - Nawapol Thamrongrattanarit
Nominated: Lino Brocka Award International Competition - Nawapol Thamrongrattanarit
Five Flavours Film Festival 2013
Won: New Asian Cinema Best Film - Nawapol Thamrongrattanarit (director)
Göteborg Film Festival 2013
Nominated: International Debut Award - Nawapol Thamrongrattanarit
Hong Kong Asian Film Festival 2012
Won: New Talent Award - Nawapol Thamrongrattanarit
Nantes Three Continents Festival 2013
Nominated: Golden Montgolfiere - Nawapol Thamrongrattanarit
Pusan International Film Festival 2012
Won: FIPRESCI Prize - Nawapol Thamrongrattanarit
Won: New Currents Award - Nawapol Thamrongrattanarit
Rotterdam International Film Festival 2013
Nominated: Tiger Award - Nawapol Thamrongrattanarit
The Thai independent film scene is relatively small and needs all the support it can get in order to survive. If you like these films, please consider supporting the filmmakers by purchasing the DVDs: www.day-for-night.org/day-shop/
Show your support by liking the Facebook page for the film: www.facebook.com/36MaryIsHappy
This film is part of a 2 DVD Set. The other film, Mary is Happy, Mary is Happy (2013), is available here.
Thanks to DJPS at ADC
These files can all be downloaded at Usenet.
36.2012.PAL.DVD5.rar – bytewhale – MirrorGet 14 Days for free Access to USENET. Free 300 GB Unlimited downloads!


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Here is my dragster running on MS2 about 2 years ago, thought I would post up some pics. The LowZ boards from Jean work great.
It was a bit of a challenge getting the IR manifold working right but once I figured out the tricks and made some mods life got easier.
Rear engine dragster
3 posts • Page 1 of 1
Re: Rear engine dragster
that is beautiful
Re: Rear engine dragster
Can you supply more info on the intake. Great looking setup.
3 posts • Page 1 of 1
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Body In Balance
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|Body in Balance
|2 June 2008 (UK)
|Owned by
|Body In Balance Limited
|Audience share
|0.0% (March 2009, BARB)
|Astra 1L
|Atlantic Bird 2
|CanalDigitaal (Netherlands)
|Channel ?
|Kabel BW (Germany)
|Channel ?
|Kabel Deutschland (Germany)
|Channel ?
|Kabelkiosk (Germany)
|Channel ?
|CAIW (Netherlands)
|Channel ?
|Hansenet (Germany)
|Channel ?
|Alice (Germany)
|Channel ?
|Arcor (Germany)
|Channel ?
Body in Balance (BiB) is a TV channel which broadcasts from London, UK. It was the UK's first health and wellbeing channel.
It airs programming about yoga, pilates, meditation, health & spiritual well being. There are regional versions of the channel for Germany and Switzerland, the Netherlands and Belgium, Luxembourg, Serbia and, more recently, Greece. In 2014, the channel plans to launch an online video on demand service.
Body in Balance was established in 2005 as a German-language channel. An English-language version, the UK's first leisure and health channel, followed in 2007. The channels were founded in response to the increasing popularity of yoga in both countries.
Since 2008 the channel's European network has grown from four to 30. The UK version was broadcast in the UK, Ireland, and 12 other countries in Europe and south east Asia. The UK version was removed from Sky channel 275 on 13 February 2012 and its satellite feed on Eutelsat 28A also ceased broadcasting.
In November 2011, Body In Balance entered administration, with the channel to be sold as a going concern.[1] Chief operating officer, Gerald Smith, left the company after concerns were raised over his employment despite being banned from acting as a director until 2021.[2]
The company was purchased in November 2011 by Alfra TV Ltd and continues to broadcast in over 20 countries worldwide.
As of 2014, Body in Balance is now owned by Body in Balance Networks and continues to be grow.
Body in Balance describes itself as 'Europe's leading wellness and yoga channel'. The channel has 10 million viewers internationally, across 19 countries and 26 platforms.
In the UK and worldwide, a large proportion of the channel's audience are professionals with an enthusiasm for health and fitness. The channel estimates that in the UK alone there are 9 million professional women with an enthusiasm for fitness, and has announced its intention to further focus on this demographic for future expansion.
The channel has worked with a number of notable teachers and instructors. Their yoga instructors include Maya Fiennes and Shiva Rea. Lynne Robinson, described by The Times as 'the Queen of pilates' has also taught for the channel, as has Doug Robertson, who is known for his Perfect Bum series. Wellness artists who have worked with Body in Balance include Danielle Collins, Lara Baumann and Ramona Braganza.
The channel also works with a number of experts in the field of diet and wellness including Maryon Stewart, who pioneered the field on non-drug medicine in Australia.
The company has a back catalogue consisting of over 400 hours of material. They license programmes and programme packages to producers throughout the world; recently the channel has made large sales in India and China.
As well as commissioning and acquiring programmes, the channel is increasingly involved in producing them and has in-house producers and post production facilities
DVD distribution[edit]
Body in Balance has a DVD library of over 40 titles and releases two new ones each month. In the UK, the channel sells to high street stores and supermarkets through RSK, a major distributor. The channel also sells DVDs from its website shop, and on Amazon and other major online shops. In Germany, they have an agreement with Rough Trade, a major retailer. In the US, Body in Balance products appear on Amazon.com
The channel has been a media partner to a number of health and fitness events in the UK and America. In 2010 they were a partner to Yoga on the Lawn in New York. 10,000 people attended the event in Central Park. In 2011 the channel will be an official media partner to the Wanderlust Festival, a festival of yoga and music held in various locations across the U.S.A.
In the UK, the channel was a media partner to the Vitality Show, which took place at the Earl's Court exhibition centre in West London. The event was marketed as 'the ultimate girls day out'. This summer, Body in Balance is holding a Summer Solstice Yoga Workshops with Shiva Rea in ZSL London Zoo.
Body In Balance operates two satellite feeds. Eutelsat's 9A delivers to Europe and Eastern Europe in German. In 2010 the company started broadcasting on Chinasat, which currently delivers to Taiwan and the Pacific in Mandarin.
- ^ "Buyers sought for Body in Balance TV channel". Business Sale Report. 18 November 2011.
- ^ "Convicted fraudster Gerald Smith admits media company Body in Balance 'close to collapse'". The Daily Telegraph. 3 November 2011.


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Posted May 13, 2016 by Aviation, Travel and Conservation News - DAILY from Eastern Africa and the Indian Ocean islands in Uncategorized. Leave a Comment
Demand for Fred. Olsen Cruise Lines' first-ever 'Islands of the Indian Ocean' fly-cruise, which was launched on 3rd May 2016, was unprecedented in the company's history, and meant that the cruise was almost fully-booked in under ten hours! In response to those customers who were disappointed at not being able to book this exotic cruise holiday, Fred. Olsen has launched two new itineraries on board 880-guest Boudicca in Autumn / Winter 2017, to explore the much-sought-after Indian Ocean region: a new 33-night sailing to the 'Mediterranean, Zanzibar & Mauritius', departing from Liverpool on 14th October 2017, and a new 15-night 'Islands of the Indian Ocean' fly-cruise, ex London / Manchester to Port Louis, Mauritius on 16th November 2017.
Nathan Philpot, Sales and Marketing Director for Fred. Olsen Cruise Lines, said:
'We are sorry that we had to disappoint so many of our guests when we launched our original 'Islands of the Indian Ocean' fly-cruise at the beginning of May; we received thousands of calls for this one itinerary to our Reservations Centre and, unfortunately – despite our best efforts – we were completely overwhelmed by the response. It is clear that we significantly underestimated the demand for this type of holiday. After listening to our customers, we have now organised two new fly-cruises to this hugely-popular region, so that our loyal guests who missed out on the first sailing now have two further opportunities'.
Details of Boudicca's two new Indian Ocean itineraries in late 2017 are as follows:
– 33-night D1724 'Mediterranean, Zanzibar & Mauritius' fly-cruise, ex Liverpool on 14th October 2017 (flying back into London / Manchester).
Ports of call: Liverpool • Cadiz, Andalusia, Spain • Palma, Mallorca, Spain • Valletta, Malta • Iraklion, Crete, Greece • Suez Canal Transit • Sharm el-Sheikh, Egypt • Salalah, Oman • Zanzibar, Tanzania (overnight) • Mozambique Island, Mozambique • Toamasina (Tamatave), Madagascar • Port Reunion, Reunion • Port Louis, Mauritius
– 15-night D1725 'Islands of the Indian Ocean' fly-cruise, ex London / Manchester to Port Louis, Mauritius on 16th November 2017.
Ports of call: Port Louis, Mauritius (overnight) • Praslin Island, Seychelles (overnight) • Cruising Silhouette Island at sunset • Victoria, Mahé, Seychelles (overnight) • Mamoudzou, Mayotte, Comoros • Hell-ville (Andoany), Nosy-Be, Madagascar • Diego Suarez (Antsiranana), Madagascar • Port Reunion, Reunion • Port Louis, Mauritius
For guests who book these exclusive new fly-cruises by 31st May 2016, Fred. Olsen will cover the cost of their gratuities and offer a 50% discount on cruises departing between 7th October and 16th December 2016 (excludes 'Party Nights').
Guests can also choose to upgrade to Fred. Olsen's great-value 'all-inclusive' drinks package for just £10 per person, per night.
To have some more information, visit: http://www.fredolsencruises.com
About the Vanilla Islands
The Vanilla Islands is an organisation that promotes the Indian Ocean islands: Comoros, Madagascar, Maldives, Mauritius, Mayotte, Reunion island and Seychelles
Follow the news on social networks:
#ilesvanille #vanillaislands
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text:
While the War of Independence began with the Battle of Lexington and Concord, the American Revolution is said to have started with the .
Check all that apply as reasons for the American colonies to become independent from Britain.
"No Taxation without Representation"
the Boston Massacre
lack of currency
Stamp Act
Quartering Act
death of Samuel Adams
Which of the following is the 2nd Estate?
The three values of the French Revolution are Liberté, égalité, fraternité. The English translation of these words into English are .
Which document does the Declaration of the Rights of Man and Citizen closely resemble?
US Constitution
Declaration of Independence
Constitution of 1801 (Haiti)
Napoleonic Code
When other countries went to war with France, they did so in order to restore the French monarchy to power.
Which of the following is NOT true about the Reign of Terror?
most people that died were from the former Third Estate
it began with the execution of Louis XIV
those responsible were known as the Committee of Public Safety
occurred during the Napoleonic Age
The king was above the Estates system.


text:
Reviews For
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I added this product and the night time moisturizer, and am very happy with them. They apply easily and my skin feels softer. Its expensive for me so I will be watching for specials.


text: ProtectWise has developed an innovative platform that addresses the urgent need for enhanced network visibility and forensic capabilities. ProtectWise sensors are deployable across all network infrastructures, including traditional ingress and egress, perimeter, cloud environments and industrial control systems. With real-time and retrospective breach detection, a rich analytics platform, workflows for optimized incident response and unparalleled visibility in a single pane of glass, ProtectWise is gaining substantial traction as a must-have for every SOC.


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Like i wanna take her out. And maybe buy her stuff. Im thinking like movies dinner /lunch shopping and like a actvity like ice skating or clubbing of. Whatever.
We hardly get to see each other becuase i wotk 6 days week a full time and a part time and trying to start my own business.
She got school and work. We live kinda far not crazy far but a little out the way but im crazy about her.
We mostly face chat and we hangout when we can for a cluple hours. And we usally go on dates 1 once a week.
But im off for 3 days and i wanna take her out for 2 dates. Ill be at her house for 2 days but will 300 be enough to cover 2 dates. Maybe 400/500
We only been dating for 2 months
Three-hundred dollars
Most Helpful Girl
Um. What? I'd slap a man for thinking of spending that much on me.
That's ridiculous and wtf do you need that much for a date?
We can:
Go to a cheap restaurant.
Netflix and pray (no chill, sorry lol)
Go to an arboretum
Go to a museum.
Walk around the beach or park even.
A surprise picnic at the park is always nice.
Go to see a movie.
Even $100 is excessive in my opinion.00|00|0
Most Helpful Guy
Fuck that. Get a bottle of wine, grab some flour from the cupboard, buy some yeast and turn on youtube to learn how to bake some bread. Plan a picnic. Afterwards, she'll be intrigued about your baking skills; say this is your first time at it and that she should help out next time. Then, using leftover ingredients, bake some more bread at the crib with her while Netflixin' and chillin'. $20 total for two dates with the added benefit of demonstrating ambition.00|00|0
ehh those are good ideas but honestly too cheap. not special enough. if they saw each other every day then yeah that would be a perfect idea because why not just chill ones in a while and not actually go out. a picnic is something to do when both people are broke, and watching netflix is the most casual thing ever. most girls actually want to go out so they can dress up and go somewhere new or try something new (to eat), not sit around in their sweatpants and eat bread.


text:
Tuesday 26 March 1963
It was Tuesday, under the sign of Aries. The US president was John F. Kennedy (Democratic).
Famous people born on this day include Paul de Leeuw and Rebecca Twigg.
In that special week of March people in US were listening to He's So Fine by The Chiffons.
In UK Summer Holiday by Cliff Richard was in the top 5 hits.
The Birds, directed by Alfred Hitchcock, was one of the most viewed movies released in 1963
while Raise High The Roof Beam, Carpenters by J.D was one of the best selling books.
On TV people were watching Crane.
But much more happened that day: find out below..
You can also have a look at the whole 1963 or at March 26 across the years.
Old Newspapers
Have a look at the old newspapers from 26 March 1963 and get them!
Which were the most popular movies released in the last months ?
TV Series
Which were the most popular TV series released in the last months ?
Find out your future
Get a FREE Numerology report based on the digits of 26 March 1963!
Wish him/her..
Historical Events
Which were the important events of 26 March 1963 ?
- Saint Emmanuel (died 304)
- Saint Ludger
- Saint Larissa
- Prince Kuhio Day in Hawaii
- Zoroastrianism - Prophet Zoroaster's Birthday.
Famous Birthdays:
- Paul de Leeuw: Dutch TV host (Cry of the Lion).
- Rebecca Twigg: American professional cyclist who was awarded the Silver Medal in the Olympic Road Race in 1984 plus many other prizes.
- Roch Voisine: Canadian singer, songwriter, actor, and radio and television host, Voisine writes and performs in both English and French.
- Natsuhiko Kyogoku: Japanese author.
Music Charts
Which were the top hits in that special week of March 1963 ?
Top #10 songs in the USA
- The Chiffons - He's So Fine
- Ruby and the Romantics - Our Day Will Come
- Skeeter Davis - The End Of The World
- The Orlons - South Street
- Bobby Darin - You're The Reason I'm Living
- The Cascades - Rhythm Of The Rain
- Roy Orbison - In Dreams
- Jackie Wilson - Baby Workout
- Bill Pursell - Our Winter Love
- Eydie Gorme - Blame It On The Bossa Nova
Search For Your Ancestors
Subscribe to our Newsletter
Don't miss our monthly news, no spam guarantee Click here to Subscribe
...and if 26 March 1963 was your Birth Date then Join our Birthday Club!
Make this date unforgettable
Genealogy: find out your family heritage
Magazine Covers
What news were making the headlines those days in March 1963?
- SportsIllustrated: Sports Illustrated: no.7812
- Life: Polaris Submarine Commander
- Time: TIME Magazine Cover: Cassius Clay -- Mar. 22, 1963
Which were the most popular books released in the last weeks ?
Remember when
What videogames would you be playing back in the days ?


text:
Mitochondria play a critical role in mediating the cellular response to oxidants formed during acute and chronic cardiac dysfunction. It is widely assumed that, as cells are subjected to stress, mitochondria are capable of drawing upon a 'reserve capacity' which is available to serve the increased energy demands for maintenance of organ function, cellular repair or detoxification of reactive species. This hypothesis further implies that impairment or depletion of this putative reserve capacity ultimately leads to excessive protein damage and cell death. However, it has been difficult to fully evaluate this hypothesis since much of our information about the response of the mitochondrion to oxidative stress derives from studies on mitochondria isolated from their cellular context. Therefore the goal of the present study was to determine whether 'bioenergetic reserve capacity' does indeed exist in the intact myocyte and whether it is utilized in response to stress induced by the pathologically relevant reactive lipid species HNE (4-hydroxynonenal). We found that intact rat neonatal ventricular myocytes exhibit a substantial bioenergetic reserve capacity under basal conditions; however, on exposure to pathologically relevant concentrations of HNE, oxygen consumption was increased until this reserve capacity was depleted. Exhaustion of the reserve capacity by HNE treatment resulted in inhibition of respiration concomitant with protein modification and cell death. These data suggest that oxidized lipids could contribute to myocyte injury by decreasing the bioenergetic reserve capacity. Furthermore, these studies demonstrate the utility of measuring the bioenergetic reserve capacity for assessing or predicting the response of cells to stress.
- cardiomyocyte
- extracellular flux
- glycolysis
- heart
- lipid peroxidation
- mitochondrion
- oxidative stress
Defining the role of mitochondria in various cardiovascular pathologies is currently an area of great interest, with many studies focusing on the properties of the organelle isolated from diseased or stressed cardiac cells. These studies now point to the importance of understanding how changes in isolated mitochondria translate to changes in bioenergetic events that take place in the myocardium during ischaemia/reperfusion and heart failure. Cardiac tissue is rich in mitochondria, which are capable of dynamically responding to energy demand for increased work. This oxidative phenotype allows for rapid and substantial ATP production for cardiac function. It is clear from experiments using 31P-NMR that, even under an increased work load in the physiological range, mitochondria appear to have a substantial 'reserve capacity' which is depleted under conditions of severe stress such as pressure overload or ischaemia [1,2]. However, this concept has proved technically difficult to examine using direct indices of mitochondrial function in intact myocytes.
Results from mitochondria isolated from hearts subject to stress typically show changes in the activity of the respiratory chain, but the functional impact remains unclear once the organelles are removed from their cellular context [3,4]. It is frequently the case that the activity of respiratory chain complexes of isolated mitochondria such as complex I are decreased in the diseased heart [5,6]. It is challenging to extend these changes to the cellular setting since the control over metabolism is lost in isolated mitochondria. For example, it is known that the maximal capacity for oxidative phosphorylation is higher than that used under normal conditions [2]. This raises the question of the role of this reserve capacity and leads to the hypothesis that it is required by cells to respond to stress and that pathological events occur when this bioenergetic reserve is depleted.
The requirement of the reserve capacity for the response to stress, such as occurs in the ischaemic and failing heart, has not been examined in intact cardiovascular cells or tissues. Since the diseased heart is associated with increased oxidative stress, exposure of cells to reactive species generated during pathology can be used as a model of pathology and to test the role of oxidative stress in myocyte dysfunction. The significance of these reactive species is revealed in studies showing that overexpression of antioxidant enzymes such as Mn-SOD (superoxide dismutase) [7], catalase [8], extracellular-SOD [9] or glutathione peroxidase [10] protects the heart from ischaemia/reperfusion injury. Moreover, partial deficiency of Mn-SOD [11] or the complete absence of glutathione peroxidase [12] or CuZn-SOD [13] renders the heart more sensitive to injury. Interestingly, volume and pressure overload [14–16] as well as ischaemia [17,18] result in an increased ability of myocytes to consume oxygen, which might suggest a mechanism for further depletion of the bioenergetic reserve capacity in an environment where oxygen availability may be already limiting.
Secondary products of oxidative stress such as HNE (4-hydroxynonenal) are normally detoxified by energy-requiring processes; however, under pathological conditions, these detoxification pathways fail, leading to accumulation of oxidized lipids [19] that can damage key proteins in the mitochondrial respiratory chain. In humans and animal models, downstream products of oxidative stress such as oxidized lipids are abundant in the ischaemic [19,20] and failing [21,22] heart. Therefore, the generation of lipid peroxidation products capable of reacting with cellular nucleophiles could be primary instigators of tissue injury [23]. The α,β-unsaturated aldehydes (such as HNE) are likely to be the most significant because they modify proteins which affect energy production [24–30] and cell death pathways [31,32]. This is corroborated by studies showing that activation of a key enzyme required for the mitochondrial detoxification of HNE [19] protects the heart from injury [33,34].
These findings raise two important questions. (i) Does bioenergetic reserve capacity exist in cardiac myocytes? (ii) Does it modulate the response to stress-associated pathology? The recent availability of technology which allows for the non-invasive measurement of mitochondrial respiration and glycolysis offers the opportunity to address these questions. Therefore we hypothesized that maintenance of mitochondrial function and the availability of a bioenergetic reserve capacity is critical to combat oxidative stress and that, when exceeded, protein damage and cell death occurs. To test this hypothesis, we used an emerging technology, high-throughput XF (extracellular flux) analysis, to quantify the bioenergetic changes that occur in intact cardiac myocytes exposed to HNE. By measuring XF, we were able to measure oxygen consumption and proton production, indicative of oxidative phosphorylation and glycolysis respectively, in intact NRVMs (neonatal rat ventricular myocytes) exposed to HNE.
Our results from the present study support the hypothesis that pathologically relevant concentrations of oxidized lipids exhaust the reserve capacity of mitochondria. When this capacity is depleted, cellular injury occurs accompanied by decreased mitochondrial oxygen consumption, decreased efficiency due to proton leak and increased protein–HNE adduct formation. These results directly demonstrate the presence of a bioenergetic reserve capacity in intact myocytes. Furthermore, these findings suggest that oxidized lipids such as HNE, which accumulate in the heart during ischaemia and heart failure, could cause, accelerate or worsen myocardial injury by diminishing this reserve capacity.
Reagent HNE was obtained from Calbiochem. All materials and reagents for the XF assays were from Seahorse Biosciences. Nonanal, oligomycin, thiazoyl blue tetrazolium, FCCP (carbonyl cyanide p-trifluoromethoxyphenylhydrazone) and antimycin A were from Sigma. Secondary HRP (horseradish peroxidase)-linked antibodies and antibodies against actin were from Cell Signaling Technology. Anti-protein–HNE antibodies were a gift from Dr Sanjay Srivastava (Division of Cardiology, University of Louisville, Louisville, KY, U.S.A). ECL® (enhanced chemiluminescence) Plus reagents were from GE Healthcare.
NRVM primary cultures
All animal experiments were approved by the University of Alabama Institutional Animal Care and Use Committee and conformed to the Guide for the Care and Use of Laboratory Animals, published by the National Institutes of Health (NIH Publication No. 85–23, Revised 1996). Primary cultures of NRVMs were obtained from 2–3-day-old neonatal Sprague–Dawley rats and were cultured as described previously [35]. NRVMs were seeded at 75000 cells/well on to collagen-coated Seahorse Bioscience V7 culture plates in growth medium containing 15% FBS (fetal bovine serum) on the first day. The next day, medium was replaced, and cells were grown in the culture growth medium without FBS. Within 1–2 days of isolation, a confluent monolayer of spontaneously beating NRVMs formed, and cells were used as described below.
Bioenergetic measurements
An XF24 Analyzer (Seahorse Biosciences) was used to measure bioenergetic function in intact NRVMs. The XF24 creates a transient 7 μl chamber in specialized microplates that allows for OCR (oxygen consumption rate) and ECAR (extracellular acidification rate) or PPR (proton production rate) to be monitored in real time [36]. For all bioenergetic measurements, the culture medium was changed 1 h prior to the assay run to unbuffered DMEM (Dulbecco's modified Eagle's medium, pH 7.4) supplemented with 4 mM L-glutamine (Gibco). First, the optimum number of cells needed for these experiments was determined. NRVMs were seeded to a density of 25000, 50000 or 75000 cells/well. Oxygen consumption in these cells was linear with respect to cell number within this range (Figure 1A), and a seeding density of 75000 cells/well was chosen for the remainder of the experiments.
Next, an assay was developed to measure indices of mitochondrial function. Oligomycin, FCCP and antimycin A were injected sequentially through ports in the Seahorse Flux Pak cartridges to final concentrations of 1 μg/ml, 1 μM and 10 μM respectively. This allowed determination of the basal level of oxygen consumption, the amount of oxygen consumption linked to ATP production, the level of non-ATP-linked oxygen consumption (proton leak), the maximal respiration capacity and the non-mitochondrial oxygen consumption. As shown in Figure 1(B), three basal OCR measurements were recorded prior to injection of oligomycin. After mixing and recording the oligomycin-insensitive OCR, FCCP was injected and another OCR measurement was recorded. The OCR measured after FCCP injection represents the maximal capacity that cells have to reduce oxygen under the experimental conditions. Finally, antimycin A was injected to inhibit the flux of electrons through complex III, and thus no oxygen was further consumed at cytochrome c oxidase. The remaining OCR determined after this treatment is primarily non-mitochondrial and could have been due to cytosolic oxidase enzymes.
Protein–HNE and GSH measurements
Protein–HNE adducts were detected using a polyclonal anti-protein HNE antibody, as described in [37]. Briefly, cells were lysed in the 24-well XF plates using 20 μl/well of lysis buffer containing 20 mM Hepes, pH 7.0, 1 mM DTPA (diethylenetriaminepenta-acetic acid), 1% Nonidet P40, 0.1% SDS and protease inhibitor cocktail (Roche). The lysates were then added to SDS Laemmli Buffer, and the proteins were separated on 10% polyacrylamide/SDS gels. After transfer on to PVDF membranes, HNE-modified proteins were detected using anti-protein–HNE primary and HRP-linked anti-rabbit secondary antibodies by chemifluorescence on a Typhoon Variable Mode Imager (GE Healthcare). Relative levels of protein–HNE adducts were quantified by densitometry using ImageQuantTL software (GE Healthcare). Total glutathione (GSH+GSSG) was measured in cell lysates with and without treatment with HNE using the Tietze recycling assay [38].
Cell death assays
Cell viability was measured using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide] assay as described previously [39] with the following modifications. NRVMs were seeded as described above into V7 culture plates at 75000 cells/well. At 8 and 16 h after HNE treatment, the assay medium was replaced with medium containing 0.4 mg/ml thiazoyl blue tetrazolium. The cells were allowed to incubate in a non-CO2 incubator at 37°C for an additional 2 h. The medium was removed, and the resulting formazan crystals were solubilized in 250 μl of DMSO. The absorbance was read at 550 nm and the results are expressed as a percentage of control.
Statistical analysis
Data are reported as means±S.E.M. Comparisons between two groups were performed with unpaired Student's t tests. Comparisons among multiple groups or between two groups at multiple time-points were performed by either one-way or two-way ANOVA, as appropriate. P<0.05 was considered statistically significant.
Protein HNE adducts and cell death increase with HNE treatment
NRVMs were seeded at 75000 cells/well and then treated with either HNE or the non-electrophilic C9 lipid analogue of HNE, nonanal, for 2 h. For these experiments, we used concentrations of HNE that have been shown to occur in the ischaemic and failing heart [19,21,40,41]. The cells were harvested and proteins were separated by SDS/PAGE followed by Western blotting with anti-protein–HNE antibodies. As shown in Figures 2(A) and 2(B), HNE adducts accumulated in a concentration-dependent manner. The non-protein reactive analogue of HNE, nonanal, was used as a structural control and did not promote the formation of protein–HNE adducts. Additionally, cell death as a result of HNE treatment was examined at 8 and 16 h by the MTT assay. HNE exhibited both a concentration- and time-dependent effect on NRVM viability (Figure 2C). HNE can be metabolized by a number of enzymes in the cell, including the GSH-dependent transferases [19,42]. Accordingly, we measured total glutathione (GSH+GSSG) levels in the lysates of cells treated with HNE for 90 min (Figure 2D). As anticipated, HNE treatment depleted glutathione, and this was maximal at the lowest concentration of HNE tested.
Effect of HNE on aerobic metabolism in intact neonatal cardiomyocytes
To determine if bioenergetic derangements preceded cell death due to HNE, we assessed oxygen consumption in NRVMs treated under the same conditions as shown in Figure 2. After basal oxygen consumption measurements, HNE was injected to give final concentrations of 0–30 μM. Control wells received vehicle (ethanol) or the structural control for HNE, nonanal (10 μM), in DMEM. As shown in Figures 3(A) and 3(B), HNE initially increased OCR in a concentration-dependent manner. Myocytes treated with the lowest concentration of HNE tested (5 μM) resulted in a steady increase in the rate of mitochondrial oxygen consumption which plateaued after approx. 150 min of HNE exposure. Exposure to higher concentrations of HNE (10–30 μM) resulted in a maximal OCR that declined thereafter. Apparent maximal increases in OCR were reached with 10, 20 and 30 μM HNE approx. 130, 70 and 35 min after injection respectively. Interestingly, the rate of increase in OCR (i.e. the slope of the initial stimulation of OCR by HNE) increased linearly with HNE concentration (Figure 3B). Nonanal was used as a structural control for non-electrophilic effects of HNE and did not affect oxygen consumption by the myocytes (results not shown).
To quantify the overall increase in the amount of oxygen consumed by the myocytes due to each treatment, the OCR area under the curve for each group was calculated by multiplying the group average by the time interval for that rate and subtracting the baseline rate. The resulting value can be expressed in pmoles of oxygen consumed during the assay after injection of vehicle, nonanal or HNE. As shown in Figure 3(C), exposure of the myocytes to HNE at 0–20 μM concentrations resulted in up to approx. 20 nmoles of oxygen consumed during the course of the experiment. The highest concentration of HNE used, i.e. 30 μM, showed no overall increase in oxygen consumption. At this concentration, OCR increased transiently, followed by a rapid decrease in oxygen consumption that was well below baseline (Figure 3A).
Effects of HNE on glycolysis
In addition to OCR, the XF assay also allowed the measurement of protons that were produced by the cells, which reflects lactate production and is therefore an index of glycolysis [36,43]. Figure 4(A) shows the ECAR profile of control myocytes and myocytes treated with 0–30 μM HNE. Similar to OCR, HNE increased the ECAR in a concentration- and time-dependent manner. Analogous to the OCR results (Figure 3A), ECAR showed a biphasic response in the 10 and 20 μM HNE groups, indicating severe damage to bioenergetic components at later time points. As shown in Figure 4(B), the initial rate of increase in ECAR was linear and dependent on the HNE concentration. Similar to that shown for OCR in Figure 3(C), the area under the curve of the PPR was calculated to determine the overall increase in proton production over the time course of the experiment. HNE treatment resulted in an increase in proton production, indicative of an increase in glycolysis, over the course of the experiment (Figure 4C). The maximal rate of proton production occurred in cells treated with 7.5–10 μM HNE.
Identification of mitochondrial defects incurred by HNE
Approx. 70 min after HNE addition (indicated by the dotted line in Figure 5B), the ECAR and OCR were used to obtain a 'metabolic image' of the cells in each treatment group. The image is divided into four quadrants, which relate the relative activities of the glycolytic and aerobic metabolism in response to HNE. As shown in Figure 5(A), HNE concentration-dependently stimulates both glycolysis and the aerobic consumption of oxygen, consistent with an increased energy demand of the cells as they respond to this stress.
To determine the specific mitochondrial derangements that occur in response to HNE, we used the mitochondrial function assay described in Figure 1(B). For these experiments, myocytes treated with HNE to final concentrations of 0–20 μM were exposed to oligomycin, FCCP and antimycin A at the time points indicated (Figure 5B). The measurements taken after each injection were used to calculate the ATP-linked OCR, proton leak, reserve capacity and non-mitochondrial OCR at 2 h after the addition of HNE. As shown in Figure 6(A), OCR was stimulated in response to HNE by 263.1±22.5 pmol/min above the baseline levels. Upon the addition of oligomycin, Figure 6(B) shows that the oligomycin-insensitive OCR in the presence of HNE increased significantly by up to 2.5-fold. To determine the individual parameters of mitochondrial function, the data shown in Figure 5(B) were analysed as described in Figure 1. Proton leak was increased by HNE in a concentration-dependent manner. Interestingly, the rate of ATP-linked oxygen consumption showed a biphasic response (Figure 6D), where it increased by approx. 3-fold with 10 μM HNE and decreased at 20 μM. These results suggest that HNE increases OCR both by increasing proton leak (thereby decreasing mitochondrial efficiency) and by increasing energy demand.
Next, the maximal respiratory rate was determined from the FCCP-stimulated rate. As shown in Figure 5(B), HNE at 0–10 μM concentrations had no effect on this maximal respiratory rate. However, concentrations of HNE in excess of 10 μM resulted in an inhibition of oxygen consumption even after FCCP addition, suggesting overt damage to mitochondrial respiratory complexes (Figure 5B). The reserve capacity was calculated based on the OCR immediately prior to oligomycin addition and the maximal respiratory rate. As shown in Figure 6(E), HNE decreased the mitochondrial reserve capacity in a concentration-dependent manner consistent with damage to the respiratory chain associated with increased protein adducts (Figure 2A). The rate of oxygen consumption due to non-mitochondrial sources was determined in the presence of HNE using antimycin A and was unchanged (Figures 5B and 6F).
Using XF analyses, we evaluated the effects of the electrophilic lipid HNE on bioenergetic function in intact myocytes. We show for the first time that oxidized lipids, at concentrations that accumulate under pathophysiological conditions in the heart, increase cardiomyocyte oxygen consumption and deplete the bioenergetic reserve capacity. We found that HNE stimulated the rate of cellular oxygen consumption in a concentration-dependent manner. This increase in oxygen consumption was due to both increased bioenergetic demand placed on the cardiac myocytes and decreased mitochondrial efficiency (i.e. proton leak). Glycolytic flux was also increased in response to HNE treatment, underscoring the integrated nature of the metabolic response to energy demand and expenditure in the presence of reactive lipid species. The HNE-induced increase in oxygen consumption was not compensated by an increase in the maximal respiratory capacity of the myocytes; consequently, HNE treatment resulted in depletion of the bioenergetic reserve capacity, overt respiratory failure and cell death.
In the heart, mitochondria make up at least 20% of the myocyte volume [44,45] and provide the unremitting energy required for contraction. Aerobic respiration is therefore indispensable to sustain cardiac function and viability. At rest, the heart consumes up to 0.15 ml of oxygen/min per g of tissue, which increases several fold with vigorous exercise by calling upon the reserve capacity of the mitochondria and glycolysis [46]. Interestingly, the diseased heart requires more oxygen to meet energy needs, which has led to the hypothesis that a state of energy starvation may underlie myocardial pathology [47]. In support of this concept, myocardial oxygen uptake is increased 2-fold in patients with left ventricular hypertrophy over normal subjects [14]; there is also an increase in the myocardial uptake of oxygen in experimental hypertrophy, congestive heart failure and diabetes [15,16,48]. Furthermore, the post-ischaemic heart uses more oxygen than the pre-ischaemic heart [18], and myocytes subjected to hypoxia/reoxygenation have an increased demand for oxygen [17]. It is reasonable to conclude, therefore, that bioenergetic dysfunction and the imbalance of oxygen supply against demand is a basic defect in myocardial pathologies. However, the mechanisms underlying this increased demand for oxygen in the diseased heart are unknown. Increased tissue levels of reactive species such as HNE are associated with both acute and chronic cardiac disease [19–21,41]. If, as shown in the present study, such reactive species increase the rate of oxygen consumption and decrease bioenergetic reserve capacity in the diseased heart, this could account for the contractile dysfunction associated with pathology.
Although the idea that ROS (reactive oxygen species) play a fundamental role in cardiovascular disease is well accepted, the molecular mechanisms by which oxidative stress cause cardiac injury are not well defined. ROS can damage cellular components by direct oxidation; however, given the susceptibility of polyunsaturated fatty acids to oxidative attack, there is a strong rationale for invoking the involvement of secondary products of lipid oxidation such as HNE and acrolein in ROS-related injury. Indeed, adducts of such oxidized lipids with proteins have been detected in the diseased heart [19,20,28,30,41,49–51] and myocytes exposed to similar compounds capable of modifying proteins promote a phenotype akin to myocardial stunning [52]. It remains unclear, however, whether oxidized lipids or their protein adducts are footprints of unquenched free radicals or if they indeed cause derangements in signalling or bioenergetics.
Recent studies showing that activation of enzymes critical for HNE detoxification protect the heart from ischaemic injury suggest that electrophiles are significant contributors to myocardial damage [33,34]. Many studies have shown that HNE damages electron transport chain complexes [3,25–30], affecting both respiration and critical events such as calcium-induced permeability transition [32,53]. These studies, however, were limited by their usage of isolated mitochondrial preparations. Although useful for understanding putative defects caused by HNE, experiments with isolated mitochondria are generally performed under saturating substrate conditions and are outside their normal intracellular environment. This removes all influence of cell signaling, ion fluxes and changes in intracellular substrate metabolism (e.g. glycolysis) on bioenergetic regulation. In contrast with the HNE-induced increase in oxygen consumption found in the present study, most studies to date report that HNE primarily inhibits mitochondrial respiration. This is probably due to the fact that the majority of studies have assessed HNE-induced changes in respiration using isolated mitochondrial preparations [19,25–27].
In the present study we demonstrate that HNE increases oxygen consumption and bioenergetic demand and decreases mitochondrial efficiency (Figures 3–6). These findings suggest that oxidized lipids contribute to myocardial injury by promoting an energy crisis. In support of this concept, we show that HNE at lower concentrations initially increased the OCR (Figure 3), which was due to both increased proton leak and bioenergetic demand (Figure 6). Using the process described in Figure 1, we can assess the impact of HNE on key bioenergetic parameters in the cell. We have assumed for the purposes of this calculation that the oligomycin-insensitive OCR is attributable to proton leak in the basal condition. However, this is not strictly correct, since oligomycin has been shown to increase mitochondrial membrane potential and the resulting OCR is likely to be an upper estimate of the contribution from proton leak. Although oligomycin-dependent hyperpolarization has no impact on the conclusion that HNE increases proton leak (Figure 6C), it will affect the value of the OCR which we attribute to ATP synthesis, primarily through an underestimation of this parameter. Nevertheless, it is clear that a significant portion of the stimulation of the basal OCR was due to increased bioenergetic demand. The increased proton leak across the inner mitochondrial membrane may be mediated by uncoupling proteins or leakage through damaged respiratory complexes. In support of this, studies using isolated mitochondria have shown that HNE promotes uncoupling and mitochondrial proton leak [24,54].
The biphasic nature of the HNE-induced changes in oxygen consumption (Figures 3A and 5B) suggest at least two factors contributing to the effects of HNE. Initially, HNE increased the oxygen consumption rate in a concentration-dependent manner (Figure 3B). We propose that this is an initial adaptive response to cellular stress induced by HNE and does not involve damage to the respiratory chain. The second phase, where the OCR decreased at the higher concentrations of HNE, is probably due to damage to respiratory complexes. Another possibility is that endogenous substrates became limiting as OCR increased. This is unlikely since 20 and 30 μM HNE, which quickly reached a peak respiratory capacity in the cells (Figure 3A), resulted in a lower overall consumption of oxygen compared with myocytes treated with 5 and 10 μM HNE (Figure 3C) and decreased the ability of the cells to respire maximally when treated with the uncoupler, FCCP (Figure 5B). Furthermore, the concentrations of HNE that caused a transient breach of the reserve capacity (i.e. 10–30 μM HNE) were associated with substantially increased protein–HNE adducts, and this resulted in complete cell death (Figures 2A and 2B). As anticipated, glutathione was rapidly depleted by HNE and was maximal at the lowest concentration of HNE tested. Since the major bioenergetic changes induced by HNE occurred without a significant change in these repressed glutathione levels, it is unlikely that glutathione depletion plays a major role in the HNE-dependent bioenergetic dysfunction reported in the present study. From these results, therefore, it is likely that extensive formation of protein–HNE adducts caused damage to respiratory complexes that resulted in inhibition of electron transport.
As discussed above, HNE levels are substantially increased in multiple myocardial pathologies [19–21,41,51], suggesting that HNE promotes tissue damage and dysfunction by decreasing the bioenergetic reserve capacity. Interestingly, myocardial oxygen consumption in the pressure-overloaded heart is increased to the maximal dinitrophenol-stimulated rate during high workloads produced by catecholamine infusion [2], suggesting that reserve capacity is depleted in the diseased heart under conditions of stress. This is particular interesting since even low dose catecholamine treatment promotes lipid peroxidation and the formation of protein–HNE adducts [22,51]. Although of particular relevance to cardiovascular disease, reduced bioenergetic reserve capacity has also been recognized in neurodegeneration. Specifically, reduction in the spare respiratory capacity has been shown to regulate glutamate excitotoxicity in neurons [55,56]. Those studies suggested that dysfunction occurs when the ATP demand exceeds the maximal ATP supply put forth by glycolysis and oxidative phosphorylation, thereby implicating that the spare respiratory capacity is critical for maintaining ATP generation under conditions of increased demand [57]. Similarly, we show that the ability of cardiomyocyte mitochondria to respire collapses when the maximal reserve capacity is depleted due to the increase in oxygen consumption by HNE (Figure 6C).
Taken as a whole, these results support the view that products of lipid peroxidation, in the concentration range reported under pathological conditions, contribute to myocardial injury by promoting bioenergetic stress. By evaluating mitochondrial function in intact cells, our findings reveal for the first time a dynamic response of cardiomyocytes to HNE, where oxygen consumption is increased through increased energy demand as well as via non-ATP-linked oxygen sinks (i.e. proton leak). Furthermore, these studies provide insight into a fundamental mechanism critical to the evolution of myocyte injury, namely the oxidized lipid-induced increase in oxygen consumption and depletion of the bioenergetic reserve capacity. Consequently, these findings have implications not only for our understanding of the pathophysiological processes underlying cardiac disease, but also in other disease states associated with increased oxidative stress.
Bradford Hill, Brian Dranka and Luyun Zou performed the experiments. Bradford Hill, Brian Dranka, John Chatham and Victor Darley-Usmar designed experiments and analysed the data. John Chatham and Victor Darley-Usmar provided financial support for the study. All authors contributed to the preparation of the manuscript.
This work was supported by the National Institutes of Health (NIH) [grant number ES10167] and Seahorse Biosciences (to V. D. U.), [grant numbers HL079364 and HL67464 (to J. C. C.)], and by NIH training grants [grant numbers T32 HL07457 (to B. G. H.) and T32 HL007918 (to B. P. D.)].
Abbreviations: DMEM, Dulbecco's modified Eagle's medium; ECAR, extracellular acidification rate; FBS, fetal bovine serum; FCCP, carbonyl cyanide p-trifluoromethoxyphenylhydrazone; HNE, 4-hydroxynonenal; HRP, horseradish peroxidase; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide; NRVM, neonatal rat ventricular myocyte; OCR, oxygen consumption rate; PPR, proton production rate; ROS, reactive oxygen species; SOD, superoxide dismutase; XF, extracellular flux
- © The Authors Journal compilation © 2009 Biochemical Society


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Need for Speed: Hot Pursuit
Review by BloodGod65
"Proving The Chase is Every Bit as Good as the Catch"
Just this once, I'll forego the spiel about the steady decline of Need for Speed. After all, the series has been in a downward spiral for the entirety of this console generation and at this point, talking about it is beating a dead horse. So let's talk about something new, shall we? Something like Need for Speed regaining its former glory. Doesn't that sound nice?
From the moment Criterion was revealed to be the lead studio on the project, expectations were high. Seeing that Criterion is one of the best racing game developers out there, many were hoping that the series would experience a complete turnaround. It should come as no surprise that Hot Pursuit is the best we've seen in years. However, the full recovery of this storied franchise remains elusive due to a few poor design decisions.
In returning to the Hot Pursuit era, Criterion has ignored most of the changes made to the series between Underground and Shift. This means cars can no longer be customized, the car roster focuses almost exclusively on exotics, there is no goofy narrative tacked on, and the open world exists completely independent of the career mode rather than acting as a hub. For the most part, none of that matters.
Instead, the game uses a career structure that both resembles Hot Pursuit 2 and Burnout 3, with its map based event selections. As with Hot Pursuit 2, there are two career modes; one that places you in the shoes of the outlaw racers, and another that allows you to play as the cops. In both careers, you'll take on unique events and earn special vehicles.
Over the course of the racer career mode, you'll play through standard races, duels that pit you against a single other driver, time trials, and gauntlets, which are time trials with police pursuit. In the police career mode, there are interceptor events which charge you with taking out a single suspect and rapid response events which are essentially time trials that penalize you for hitting traffic or obstacles. Naturally, both career modes have Hot Pursuit events, which either charge you with getting to the finish line intact or taking out all racers before they escape. Unfortunately this event type is also the most infrequent, making up perhaps one in ten events in each career. What's more disappointing is that the most unexciting events the time trials are by far the most frequent. It's a shame that a game named Hot Pursuit features so little actual pursuing.
However, when you actually unlock one of these events it is an occasion worth celebrating. Regardless of which side you are on, Hot Pursuit events play out like high-speed demolition derbies. Cars crash into each other with the aim of wearing down opponents and ultimately taking them out of the fight. When a car finally succumbs to the onslaught, it crashes in spectacular fashion. Criterion's tenure with Burnout is immediately obvious in the slow-motion cutaways and the frenzied nature of these events. As exciting as they are, the intensity only rises when weapons are introduced into the mix.
While everyone has nitrous, the weapon loadouts for each group are tailored to what they do. In the case of racers, this means weapons that help evade pursuit, while the cops have weapons that are meant to stop the racers. Racers have an EMP blast ability - which damages and temporarily disables vehicles in front of them - spike strips, a jammer that leaves cops blind and disables their weapons, and a turbo boost that functions like nitrous on steroids. The cops also have EMP and spike strips, but also have the ability to call in roadblocks and helicopters. The helicopter tracks racers and is supposed to throw down spike strips, but the one time I actually witnessed this happening was when they dropped it right in front of me. The roadblock is also problematic, because the game gives you no indication of where the opening is. Even though it is meant to stop racers, it usually has more of a chance of stopping the player, while the racers slip through.
Even so, most players will learn the best ways to use the weapons and experience few problems after the initial learning curve. However, there are more significant problems to be found in Hot Pursuit. The first, and one that will strike racing fanatics as the ultimate heresy, is the lack of a manual transmission option. While Criterion has never included this option in their games, its omission from a Need for Speed is a questionable departure from the norm, to say the least. While this is initially a turnoff, especially in the beginning when the cars are slower, as things start to pick up it becomes less noticeable. Eventually everything begins to move at such a speed that you won't even notice. Like Burnout Paradise, you'll have to dedicate every bit of your attention to the road and the action at hand in order to stay in the race.
In the end, the most grievous error Criterion has made is with the vehicle physics. In a nutshell, vehicle handling feels too heavy and unresponsive. Not only is this yet another unsettling deviation from the traditional Need for Speed template, but it is utterly out of character with the buttery smooth controls of previous Criterion racers. Because of this, minute adjustments or last-ditch evasive maneuvers are nearly impossible because the vehicles refuse to respond quickly enough to the player's commands. It also becomes a hassle to negotiate the tracks as the cars tend to veer into walls or otherwise oversteer and powerslide through. Like the quirky weapons, dealing with the unusual vehicle physics eventually becomes second nature. Unlike the weapons though, something so elementary and fundamental as moving the car around should not have a learning curve not in an arcade racer at least. It is, at best, a lamentable misstep that will surely sour many people's enjoyment of the game.
Seeing as how Hot Pursuit is otherwise a very good game, it only seems fair to end the gameplay discussion on a positive note. In their previous efforts, Criterion has been unable to utilize actual vehicles (something about comprehensive destruction and reckless driving usually makes automobile manufacturers extremely uncomfortable). Now that they've finally gotten the chance to use real life cars, Criterion has created a comprehensive list of the most awesome vehicles ever to hit the streets. I'm willing to go out on a limb and call it the best list in a Need for Speed to date. The first tier cars are performance cars in and of themselves; the 370Z and the new Porsche Boxster both appear. The list quickly ramps up to include the entire current lineups of Porsche and Lamborghini. Other mentionables include Koenigsegg, Pagani, Mercedes with the SLR McLaren and the SLS and Aston Martin's One-77. There are a few things I could get nitpicky about, such as the inclusion of convertibles over their hard-topped - and better looking - counterparts, and the duplicate cars (the four versions of the Gallardo, for instance), but these are really only things that the most anal gearhead will fuss about.
While the gameplay of Hot Pursuit takes obvious cues from its predecessor, Criterion has also received inspiration from Hot Pursuit 2 in terms of its graphical design. Taking place in fictional Seacrest County, Hot Pursuit manages to encompass a lot of varied terrain, including snowy mountain passes, lonely stretches of desert, winding paths between a sequoia forest, and tight curves hugging sheer oceanside cliffs. Longtime fans will recognize these as the same types of areas that made up the tracks in Hot Pursuit 2. Far from feeling like a rehash, this simultaneously pays homage to that beloved title while updating it with the power of the current consoles. These familiar locales are now absolutely gorgeous and stunning in their detail, and it's tempting to just slow down and take it all in. Thankfully Criterion has built in an option that allows players to drive around the open world without racing just to sightsee. As an added bonus, Criterion has built in shifting times of day, so it's not uncommon to race at night. While the racing is intense enough during the day, nighttime adds a whole new dimension. It's safe to say that nighttime racing ranks right alongside Dead Space as one of the scariest and most pulse-pounding experiences I've encountered in gaming.
As great as the environs are, the cars are their equal in every way. It's been a long time since I've seen cars look this good and it is obvious that the folks over at Criterion are as car crazy as most Need for Speed fans due to the loving attention given to every model in the game.
It also seems like Criterion did its homework for the car sounds. Every vehicle has its own unique sound, and the combined cacophony of four dueling V10 engines and squealing tires creates a rousing symphony of automotive power. But like most EA games, the soundtrack is a letdown. There are some really excellent songs, but the majority of it is a bit strange. Unfortunately, Hot Pursuit doesn't have the usual EA Trax option that allows you to browse the music and turn off the songs you don't like.
Hot Pursuit is an excellent game and certainly the best we've seen in a long time, but I stop short of saying Criterion has resurrected the franchise. Although EA handing the series over to such a respected developer suggests they are finally ready to admit they've let the series slide for too long, Hot Pursuit still hasn't reached a level where it will be palatable to the gaming masses. This is due in large part to the unresponsive vehicle handling the ultimate sin for any arcade racer. Even so, those who have given up on Need for Speed in recent years and racing fans in general will want to check out Hot Pursuit. I for one hope this is the signal that Need for Speed is finally returning to its former glory.
Reviewer's Rating: 4.0 - Great
Originally Posted: 08/15/11
Game Release: Need for Speed: Hot Pursuit (US, 11/16/10)
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US public service advertising could learn from the UKBMJ 2007; 334 doi: https://doi.org/10.1136/bmj.39146.364757.DB (Published 08 March 2007) Cite this as: BMJ 2007;334:497
- Janice Hopkins Tanne
- New York
The way public service messages in the United States are communicated to the public has evolved without much planning and might benefit from the UK model, said Vicky Rideout, vice president and director of the programme for the study of entertainment media and health at the non-profit Kaiser Family Foundation. She was speaking late last month at a conference in Washington, DC, that compared public service advertising in the UK and the US.
The US system is "part paid, part donated, part run through other agencies, [and] part run through the government," Ms Rideout said. "Until very recently I had absolutely no idea that in Great Britain public service advertising is overseen by a 600 person government agency with a mandate to coordinate, implement, and oversee all of the government's public …
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Photos are for members only!!
Peachmate is a 100% free sex site for adults looking for fun with no strings attached.
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I'm a stocky guy in Penticton BC looking for someone for discreet NSA fun. I'm not bad looking, but am a bit short (5'4"tall) Never had a problem with that though. Brown hair, blue eyes.


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We love Denise O'Neill at Grey is Okay. She's got a lovely story and great message for any woman who is thinking about ditching the dye. … [Read more...]
Gorgeous Gray Hair Makeovers: Look Like a Silver Fox
Earlier this year, NBC's Today Show had a wonderful segment that featured the makeovers of three women who chose to go gray. It also includes great quick tips for gray hair care. Visit NBCNews.com for breaking news, world news, and news about the economy Here are the wonderful gray hair products used on the women (aff): Aveda Brilliant Pantene Pro V Silver Shampoo Fekkai Brilliant Glossing Cream Bumble and Bumble Dry Shampoo … [Read more...]
Going Gray…One Woman's Inspirational Story
What's your story? Here's a great one you'll want to hear! … [Read more...]
Liz Rizzo Talks about Anne Kreamer's Going Gray
Featured earlier on this blog, Liz Rizzo is a beautiful 30-something with gray hair. … [Read more...]


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Embattled Northern Territory Chief Minister Adam Giles has retained leadership of the Country Liberals government, with challenger Willem Westra van Holthe his deputy.
Mr Giles prompted a political crisis when he refused to resign on Tuesday, despite being ousted in a leadership coup on Monday night.
The late-night meeting resulted in Primary Industry and Mines Minister Willem Westra van Holthe being elected chief minister by nine of his 14 Country Liberals parliamentary wing colleagues.
But on Tuesday it became embarrassingly clear Mr Giles didn't plan to give up so easily, after word filtered out five minutes before Mr Westra van Holthe's swearing-in was due to start that Mr Giles had not resigned. The ceremony could not go ahead.
A CLP source told AAP that without at least four more CLP parliamentary wing members on board, Mr Westra van Holthe didn't have the numbers to lead the government.
Former deputy leader and Treasurer Dave Tollner opposed the change, along with Speaker Kezia Purick and members Bess Price, Peter Styles and - of course - Mr Giles himself.


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Download Burkina News apk 1.1 free for Android smartphone
Posted by News Now in News & Magazines | Sept. 27, 2015 | 0 Comments
Apk file size: 2.9 MB
Burkina News (Burkinabé) brings you the most popular newspapers from Burkina Faso in French (Française) and English. This is a fast and easy way to read the latest news. No unnecessary features, we keep it simple to you!
- Most popular newspapers.
- Automatically download latest news.
- Really fast app, and it won't drain your battery.
- Burkina News, Newspaper, Journal.
These are some of the available newspapers:
- Burkina24
- Faso Presse
- Le Faso
- L'Observateur
- aOuaga
- Fasonews.net
- Mutations
- Evénement
- Labor Presse
- Le Pays
- L'Express du Faso
- France24
- allAfrica
- LeMonde
- Le Figaro
Do you want other newspaper on the list? Do you think that some newspaper should not be on the list? Need help? Write us [email protected]
Read the most popular newspapers from Burkina Faso (Burkinabé).
Whats new
Thank you using the app. As always, we keep it up to date, recently we've check the newspaper list and everything is working as expected. Anyway, feel free to contact us if you need help!
News Now part of our News & Magazines and have average installs from 10000 to 50000. Last Update Sept. 27, 2015. Google play rating is 84.058. Current verison is 1.1. Actual size 2.9 MB.
Similar applications
Burkina Faso News - NewsPapers8 Comments


text: Located just a 10-minute walk from the centre of Arles, La Chambre d'Hôtes Arles en Provence offers B&B accommodation. You can borrow the bikes to discover the area and several hiking paths start from the property. With a private terrace, the room at La Chambre d'Hôtes Arles en Provence is decorated with a mix of modern and antique furniture, in addition to original artworks created by the owner. With a garden view, it features an independent entrance, a seating area and a desk. The en suite bathroom is fitted with a shower and a hairdryer. A continental breakfast with homemade jams and pastries is included and served daily in the dining room. A welcome tray is provided in your room and restaurants can be found 0.6 miles from the property. La Chambre d'Hôtes Arles en Provence is equipped with free Wi-Fi. Free public parking is possible in front of the property and Saint-Remy-de-Provence is 14.3 miles from the B&B. Avignon is 21.7 miles away.


text: From the chaos of the Bakumatsu, two men now face each other in the twilight of an unknown tomorrow. One man, a wanderer with a cross shaped scar on his left cheek, a reverse bladed katana, a vow to never kill again, and with the sword that gives life deep in his heart, faces off against his brutal successor, a devil of the past, bandaged head to toe, with a katana long drenched in the blood of many, and wickedly burning ambitiously aflame with hellfire in his soul, whose sword that kills lusts for power, conquest, and strength. It is now Amakaeru Ryu no Hirameki versus Kagutsuchi, and the Will to Live against Only The Strongest Survive! In the dawn of the new day that awaits, who shall be the victor?


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German carmaker Volkswagen has launched the limited edition Polo AllStar in India. Prices for the new Polo AllStar start at Rs. 7.51 lakh ex-showroom (Mumbai).
Volkswagen Polo AllStar Price List
- Petrol - Rs. 7.51 lakh
- Diesel - Rs. 9.07 lakh
All Prices - Ex-Showroom (Mumbai)
The extra Rs. 30,000 that owners pay to get the new Polo AllStar gets you get minor cosmetic changes and equipment not offered on the standard Polo.
Changes on the outside include LED headlights with headlamp washers along with 15-inch, 20-spoke alloy wheels.
Inside, the Polo AllStar hatchback features new fabric seat upholstery with a single contrasting stripe running down the centre. The seats also feature contrast stitching.
Also present are metallic finished pedals, a foot-well lamp and scuff plates bearing the AllStar badging.
Underneath the hood there are no changes at all with the 1.2-litre petrol kicking out 74bhp and 110Nm of torque and the 1.5-litre diesel engine producing 89bhp and 230Nm of torque.


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Salt and Pepper shakers American glass set, forget me not, Challinor's No. 20, opaque white, mold blown, 6 lobes, 1 7/8" tall, 1 1/2" diameter at the bottom. No damage. Different tops. Circa 1885 - 1891, made by Challinor, Taylor and Company of Tarentum, Pa. See photos.
To purchase with MC or VISA please call Drury House Antiques 802-824-4395 EST or use PAYPAL
Need help with your order, Call us 802-8244395 EST 8 AM to 8 PM
Sale Final Days


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Love cars always have been obsessed with cars and everything about them, always wanted to be a mechanic... I've always been obsessed with finding out everything about them, but i dont know enough (i know a lot... but not enough) other than family -cause mine is an asshole- how did you learn about cars because i need to know everything!! What videos do you watch, pages you follow, magazines tv shows everything... i need to learn more about cars they are my life #cars
Most Helpful Guy
Well, I mean, I had Hot Wheels and model cars and car calendars and stuff as a kid ever since I remember. So there's that.
Since my family is pretty much on the lowish end of the middle class or something, I had to work at a grocery store for minimum wage, part time, in order to save up for a car.
I ended up with about $2000 to spend, so I had to look on craigslist.
I mostly just clicked on the cars that looked cool enough, or I already knew about, and then narrowed them down by mileage and stuff. I did lots of research on the models after narrowing them down, to see if they were reliable enough.
I ended up getting a '94 Cadillac Seville as my first car, and MAN it was a fuckin sweet ride. I felt very impressive driving around in that thing. It was so classy.
Unfortunately, in 1994 they had started using the Northstar engine, which is one of the worst engines to work on. But, on most of the reviews, people talk about all the other great things... it's only when I was trying to find solutions to problems with that engine that I finally found out how shitty of an engine it was. And even then, it worked really great and efficiently until you had to fix it. In fact, I think it was a "reliable" engine for the most part, but if it broke, you went broke.
So yeah, my dad and I (mostly me doing the work until I had to ask him how to do something) fixed a lot of problems with that car, and eventually realized that the head gasket was fucked, which was the main problem for many Northstar owners, and basically meant the whole engine would have to be replaced. (And the engine would cost more than I bought the car for).
Somehow we were able to sell it, probably only because it was in PERFECT condition cosmetically, despite being 20 years old.
My parents and my brother and I have bought cars off craigslist for the past 5 years or so because that's all we could afford, and we have learned lots about cars from that...00|00|0
u just described my life lol i still collect hot wheels too and i got this nice 97' soarer with a 2jz, did some body work and a repaint.. just orderd a nice set of rims too lol thats cool tho


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CMA Intermediate Result of Dec 2016 Exam announced by ICWAI
The Institute of Cost Accountants of India (ICAI) has announced that result of CMA Intermediate examinations held in Dec, 2016 will be available on 21-Feb-2017 late evening. If you have appeared for CMA Inter Dec 2016 Term of examination, then you may check your ICAI-Cost exam result today at official websites examicai.in or examicmai.org on 21-Feb-2017.
CMA Inter Dec 2016 Result – ICWAI Exam Result 2017
Check, download and print the results for CMA Inter examinations held in June, 2016 which are likely to be available at the following web address:
Check CMA Intermediate Result
(Official Website Server 1)
CMA Inter Dec 2016 Result
(Official Website Server 2)
All CMA Intermediate qualified candidates shall be eligible for getting admission to the next level i.e. CMA Final Course. Best of luck to all candidates who are eagerly waiting for their CMA Inter Dec 2016 result. I wish all of you will definitely pass with flying colors by getting good marks and passing percentage in the ICAI (erstwhile ICWAI) examinations.
Hence, all of those candidates who have appeared for the Dec, 2016 term of CMA (Cost and Management Accountants) Intermediate examinations may check their results online on 21/02/2017 at the following websites:
- http://examicai.in/
- http://examicmai.org/
Share your result with us and give your opinion on the marks allotted for your hard work and practices for June 2016 examination.


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BD Ultra Fine insulin syringes are designed for safety and comfort. They are engineered with optimal point geometry and are coated with special Micro Bonded lubrication for a smoother entry. All BD insulin syringes are manufactured with surgical grade steel and are guaranteed to be sterile
Single-use scalpels avoid cross contamination and are suitable for simple surgical procedures.
• Precision molding of blade into handle, with a replaceable blade guard, for complete user protection.
• Comfort fit handle design for good grip and easy manipulation.
• Made of high-quality stainless steel.


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January 11th, 2012
Episode 56 of 195 episodes
Masterchef 2011 champion Tim Anderson cooks the perfect sirloin steak.
You'll need to sign in to write a comment.
Welcome to the Brain Training Podcast, the daily audio workout for your head. In this podcast we have two games for you, each with three rounds which get progressively harder. To enjoy the full experience, relax, and avoid distractions whilst you listen.


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Can you tell me who does your screenprinting and embroidery?
We house our own screenprinting and embroidery facility.
I want to work for True Feel. How do I apply for a internship/job?
We apologize, but we are not accepting any applications for internships or jobs at this time.
Do you sponsor?
We do, check out and learn all about it HERE.
I own the sweetest shop/distribution in the world…How I could stock TrueFeel in my shop/distribution?
Email email@example.com your proposal and our Sales team will send you a PDF application to fill out and send back.
I ordered from your online shop and need an exchange. What should I do?
No problem, just email firstname.lastname@example.com your order number and the product you want to exchange. As long as the item is in its original packaging and hasn't been worn or washed, we will take care of your exchange right away.
How do I know my gear is on its way to my house?
UPS/USPS tracking numbers are emailed to the email account you used when ordering with our site. You should get an email once UPS/USPS scans the package.
Do you ship internationally?
I'm an international customer. Is there still a way I can track my order?
We use USPS for ALL international orders to minimize your cost. Unfortunately USPS does not offer tracking numbers once upon scanned goods. We will email you your USPS tracking number as soon as your order is processed by USPS and we recieve the tracking information from them.
I accidently entered the wrong shipping address how do I change it?
Don't panic! Click Here to contact us with your order ID number and correct shipping address/size/style as soon as possible. We will do all that we can to reach your package before it is shipped. If your package has already been shipped, there is still a chance we will be able to correct the address before delivery. Either way, we will work with you to resolve the issue.
Do you have a Street Team I can join?
Of course! Send an email to email@example.com asking to join along with your Name and Address, and we'll get you all hooked up.
What does it mean when an item is Limited Edition?
If an item is Limited Edition, it means that there is a limited quantity made…once it's gone, it's gone.


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Talk:Chicago Cubs
From Uncyclopedia, the content-free encyclopedia
You're an idiot. It didn't stop in 1908. Try 1907. Get your facts right. And you know the Cubs have won just as many World Series as the Sox in 101 years? Just thought you'd like to know that.
edit Page update
Someone should update this page because the Cubs won this year. It's time for an update. 184.108.40.206 03:16, December 26, 2016 (UTC)
- Yes, they did. Get started, then. Spıke 03:19 26-Dec-16


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INDIA: Apollo mulls tyre price hike
Apollo Tyres, India's second largest tyre maker by sales, is considering increasing product prices next week, after rubber prices soared to a record high, a senior official said.
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didn't work for me
I have oily skin and open pores, so when I came across this product, I did not hesitate to buy it... much to my disappointment, it did not "dis-a-pore" my pores, the horror is it actually builds up in my pores over a couple of hours such that I could see little dots of the color deposited in my pores... just the opposite of what I want to achieve! ... what a complete waste of my money!
Was this review helpful?
Sandra farmer
This product really does reduce the appearance of pores. It can appear a bit mask-like at first, but the effects lessen during the day. Best of all it's a product with sunscreen that I can put on my nose, without making it look shinier and even worse than usual.
Was this review helpful?
Fantastic BB cream
This BB cream is fantastic. Perfect for oily skin. It is matte and long lasting. The only criticism I have for this product is that there's no shade option. I found it too light for my skin tone so I have to use bronzer to fix the colour.
Was this review helpful?
Dis A Pore Beauty Balm
Not a fan of this product at all. First, it doesn't blur the pores. I don't have big pores to begin with. Therefore, the fact that it doesn't do anything is very disappointing. Also, the color is too light for my nc25-30 skintone. and pink-base just doesn't suit me at all.
Was this review helpful?
Eileen lim
It's really work on to cover my pores.
This is actually my first try on Dr.Jart + product. What make I buy this is because I got a sample of it from the previous purchase. Once I started to use it and it make me fall in love on it. It really covers my open pores, and very good oil control for my combi more to oily skin. Thanks Luxola.
Was this review helpful?
Dis a pore
At first I thought this was too white, so I just used it as a primer until I discovered I was not applying it properly. When I used a sponge to dab and blend the effect was natural and I could use it alone. My skin tends to get oily so I jut use a serum under this and I am done.
Was this review helpful?
Not Bad!
This is one of the few BB creams that I've tried and really liked. It doesn't have a super grey shade like how some other BB creams do. It also covers my pores fairly well, which is pretty good considering that I have significant large pores and acne pock marks. It also stays on great in Singapore's humid weather and doesn't turn my face too oily by the end of the day!
Was this review helpful?
Dr Jart+ Dis-A-Pore
I have always been a fan of Dr. Jart+'s BB creams, and this one definitely is another good one. I like its texture once applied, and its matte finish. I highly recommend this for those who are looking for a BB Cream as part of their daily skin routine.
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Claire lee
Covers pores well
Covers pores pretty well. No need for a pore primer before. I use my fingers to dot and then spread in a patting motion. Sponges seem to absorb a lot of the product and it goes to waste. The color is a tad fair (Mac NC25) for me if I am not careful with the amount I use. A little pricey but works well.
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Sook yan siew
Tried the sample they gave and it looks fabulous on me, and I immediately bought a bottle. However, the sample and the real product actually differs and it isn't as good.. it does not last long as well.. looks great at first but gets cakey later on.. anyone have some solutions to this? Anyway, it's a no-second-tube for me. Low coverage too.
Was this review helpful?


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Proform Chrome Alternator 66445N 100 Percent New - 63 Amp - Chrome Finish - GM 1973-86 Internal Regulator
Individually Tested Pit Stop USA offers Proform Chrome Alternator 66445N at low everyday prices. Be sure to check out all of the products from Proform including Proform Electrical System Parts and Accessories, Proform Alternators and Generators, Proform Alternators.Product Description: Alternator, 80 amp, 12V, Internal Regulator, Single V-Belt Pulley, Chrome, GM
- 37995964451
- Proform Performance Parts
- Available
- Same Day Shipping!
- New
Shipping Information
- Item Requires Shipping
- 11.6 lbs.
- W6.8000" x H7.1000" x L7.1000"
- Ships from Michigan.
Customer Reviews
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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
You can make your form become invisible when the user's screen width is less than a common width for mobile devices, such as making it invisible if the device it is viewed on is less than 680 pixels wide.
To do that, we can simply inject the following custom CSS into your form:
@media only screen and (max-device-width: 680px) {
.supernova { display: none; }
However, as you are using the automatic popup of your form in a lightbox, the lightbox will still popup for users on mobile devices - only the lightbox popup would appear empty. In other words, the above method works for hiding a form which is embedded through iFrame or script methods, and will not prevent lightbox or feedback methods from popping out and bothering your users.
What I would recommend instead is for you to make your Lightbox popup mobile responsive. It is quite easy, all you need to do is set the height and width in your embed codes to false, rather than using a predefined width of 700 pixels and heigh of 500 pixels. So your lightbox embed code would look like this, with the only changes marked in red:
 Click this to show lightbox popup


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The Foundation's Board is responsible for making key decisions, setting goals and evaluating progress, setting policy, establishing committees, and ensuring all board and committee actions align with the mission, vision and values of the foundation.
Tara Wilson, PresidentEventsia Susan Smith, Vice-PresidentTwo Forks Catering Lance Hindt*, SecretaryKaty ISD Superintendent Ken Janda, TreasurerCommunity Health Choice
Rob Bamford, Cross Creek RanchChris Borreca, Thompson & Horton, LLPThomas Butts, Hargrove Engineers + ConstructorsDoyle Callender, Rise DevelopmentKeith Carmichael, Cinco Ranch High School Athletic Booster ClubTom Cline, Seven Lakes High School Fine Arts Booster Chris Crockett, Community VolunteerKeiko Davidson, Davidson ElementaryHenry Dibrell, Katy ISD Board of Trustees - MemberPaul Dickens, Sovereign Wealth Advisors LLCKerry Gilbert, Kerry R. Gilbert & AssociatesJeanne Griffith, President, Katy Council of PTAsKelly Hammons, Community VolunteerAnn Hodge, Katy Area Chamber of CommerceMatt Katsarelis, Katy MagazineYvonne Kershner, Brazos Valley Schools Credit UnionFrank Lombard, Katy Area Economic Development CouncilShani Matheson, Ex-Officio Chair, Katy ISD Volunteers in Public Schools Executive BoardBob McAuliffe, Wealth Design GroupRandy Petty, Shell Oil (retired)Jeffrey Poole, AXA AdvisorsLance Redmon, Sundance Premier Memory CareAllan Rundell, Chase BankMatt Schomburg, State Farm InsurancePatti Shafer, Fred and Patti Shafer ElementaryBrenda Shaver, Community VolunteerMatt Shivers, The StandardMaureen Singleton, Thompson & Horton, LLPKaren Thornton, Katy Area Retired EducatorsMike Van Hoozer, Van Hoozer & AssociatesMarta Vasel, BP America, Inc.Downy Vickery, Prosperity BankJamie Wolman, Ray and Jamie Wolman Elementary
AdministratorsDr. John Alawneh, Chief Information OfficerDr. Christine Caskey, Chief Academic OfficerChristopher J. Smith, Chief Financial OfficerDr. Andrea Grooms, Chief Officer, Communications, Governmental Relations & Administrative Support Campus PrincipalsAngel Bateman, Williams ElementaryDavid Paz, Mayde Creek Junior HighTed Vierling, Seven Lakes High School
Classroom TeachersStephanie Rundell, Elementary Teacher of the YearPattison Elementary Tiffany Shirey, Secondary Teacher of the YearMorton Ranch High School
Janet Theis, Director of Community PartnershipsLeah Marchione, Manager of Community & Internal RelationshipsTammy Stringer, Partners in Education Senior Specialist * indicates ex officio, non-voting members of the Board Voting members of the Board of Directors serve either one- or three-year terms. Three-year voting members are nominated by individual board members and elected by the entire board. Other voting members, who serve one-year terms, represent district organizations including the Katy Council of PTAs, Katy ISD VIPS Executive Board, a Katy ISD Athletic Booster Club and a Katy ISD Fine Arts Booster Club. These community representatives are recommended for service by the foundation board president and ratified by the board.
6301 South Stadium LaneKaty, TX 77494Contact: Janet firstname.lastname@example.com


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Question: Recall the discussion of IT professionals in Chapter 1 Business
Recall the discussion of IT professionals in Chapter 1, "Business Information Systems: An Overview." Of the following professionals, who does the majority of the systems construction job: the CIO, systems analyst, database administrator (DBA), or programmer? Why?
View Solution:
View Solution:
Answer to relevant QuestionsWhat is systems integration?You are trying to explain to your supervisor the general details of a proposed IS. The IS involves a server connecting many PCs. Your supervisoris not an IS professional and has no idea what a DFD is. How would you prefer to ...An increasing number of IS professionals prefer to call the end users of their creations "customers," even if the developers and users are employees of the same organization. Why?You are the CIO for a large university hospital. The medical staff of the cardiac ward would like to build an expert system for diagnosis. Your preliminary review shows that the financial investment would be considerable. ...Why don't users commonly document the applications they develop? Why is poor documentation a problem?
Post your question


text:
Barefoot Bandit
July 11, 2010 by Post Team
Barefoot Bandit:(FOXNews (blog)) — Police sources tell us that "Barefoot Bandit" Colton Harris-Moore is to be calm and cooperate with them.
Speaking by phone with an unidentified source, we could hear the sirens of police cars as escort a convoy of Mr Harris-Moore to Nassau, Bahamas where he faces possible extradition to the United States.
A press conference is expected at noon.
Yesterday, Fox News has obtained photos of the board of Harris-Moore is a suspect in emergency landing after flying in from Indiana with zero formal training.
Please feel free to send if you have any questions regarding this post , you can contact on
Disclaimer: The views expressed on this site are that of the authors and not necessarily that of U.S.S.POST.


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Description: The Mole Cake from DR. OETKER is a unique creation: Under the molehill is a...更多
The Mole Cake from DR. OETKER is a unique creation: Under the molehill is a wonderful cream of chocolate flakes and bananas located. The cake always works out, is quick and easy to prepare.
- with chocolate flakes and bananas
- simplest preparation
Sugar, wheat flour, 13% chocolate flakes (sugar, cocoa mass, fat reduced cocoa powder, cocoa butter, emulsifier soya lecithin, flavoring), wheat starch, 3.4% low fat cocoa, dextrose, baking powder (raising agent disodium diphosphate, sodium bicarbonate), gelatin, rice starch, modified starch, emulsifier (e 475, e 471, e 433), aroma, thickener E466
Preparation Note:
You have to add: 100g softened butter, 2 eggs, 75ml of milk, 3 bananas, 550g whipped cream, 100ml water
Product Specifications:
1 x 435g DR. OETKER Cake Mix Mole Cake