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Proteomic analysis of the marine diatom
Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants ubiquitously distributed. They are generated by incomplete combustion of organic materials such as wood or fossil fuels. Due to their carcinogenic, mutagenic effects and to their wide distribution in the environment, these pollutants pose many concerns to researchers and regulators. In our laboratories we investigated the effect of benzo(a)pyrene (BaP) exposure in the marine diatom
In order to investigate the mechanism of action of PAHs, we exposed the diatoms for 24 h to 36.45 μg/L of BaP which inhibits the growth by about 30%, and analysed the relative protein expression profile by a quantitative proteomics approach based on iTRAQ labels. The proteomics profile analysis showed that around 10% of the identified proteins were regulated and one fourth of them confirmed the gene expression changes seen by DNA microarray. Particularly interesting was the down regulation of the Silicon transporter 1 (SIT1), an enzyme that is responsible for the uptake of silicon from the media into the diatom cells. Regulation of SIT1 upon BaP treatment was also confirmed at the gene expression level.
The potential use of the regulated proteins found in this study as early indicators of environmental exposure to PAHs is discussed. In particular, SIT1 is considered a promising biomarker and SIT1 expression changes were confirmed also when the diatoms were exposed to field samples, e.g. marine surface sediments contaminated by PAHs.
Diatoms are eukaryotic, unicellular, photosynthetic organisms that inhabit marine and fresh waters worldwide and are responsible for about 40% of the total carbon fixation in oceans
The available genomic information for these diatom species, associated with the important role they play in the carbon fixation in oceans, paved the way for their use as model organisms in molecular ecotoxicology studies. Thus, our laboratory has designed a DNA microarray chip containing all the available genes in
Recent advances in shotgun proteomics and the use of isobaric labelling of peptides and proteins for the relative quantification of an entire organism or cell proteome under different experimental conditions offer promising tools for the discovery of biomarkers also at the protein level. We describe here a procedure for proteomic analysis of the diatom
Effect of BaP on diatom growth
To study the effects of BaP exposure in diatoms at the molecular level,
Figure 1
Growth curve of diatom cultures
Growth curve of diatom cultures. (A) Line and scatter graph shows the diatom culture density along culture time in BaP-exposed (open symbols, dashed line) and control (closed symbols, continuous line) conditions, in three biological replicates. The black arrow indicates the time when exposure started. (B) Vertical graph shows the diatom average decrease in the growth rate caused by BaP treatment after 24 h exposure, when compared to control conditions; error bars represent the standard deviation among the three biological replicates.
Protein identification
Protein extraction from three biological replicates, each consisting of both control and BaP-exposed conditions, was carried out on consecutive days. Additional file
Additional file 1
Workflow for the identification of protein changes induced by BaP treatment in
Click here for file
Additional file 2
Silver staining of protein extract and tryptic digest run on SDS-PAGE. The figure shows the similar amount of proteins run for three biological replicates. Control protein extracts, labeled with iTRAQ 113, 115 and 119 were loaded onto lanes 1, 5 and 9, respectively, while BaP-exposed protein extracts, labeled with iTRAQ 114, 116 and 121 were loaded onto lanes 3, 7 and 11, respectively. Tryptic digested products were loaded on the neighboring lanes to the right of the corresponding protein extracts, and show the protein digestion was complete under the used conditions. Molecular weight markers were loaded onto lanes labeled with M.
Click here for file
Additional file 3
Protein identification in
Click here for file
Protein quantification
iTRAQ-mediated shotgun proteomics allows the relative quantification of peptides from up to eight samples simultaneously, and has proven to be a successful tool in protein biomarker discovery, both in the biomedical
Different iTRAQ reagents were used to distinguish peptides obtained from diatom cells cultured in control and BaP-exposed conditions (Additional file
Table 1
Changes in the relative abundance of proteins in
Regulated proteins based on two or more peptides at >95% confidence
Gene name
C-5 cytosine-specific DNA methylase, P
Metabolic process
Mitochondrial chaperonin
Cysteine synthase
Biosynthetic process
N-acetylornithine aminotransferase
Metabolic process
3-isopropylmalate dehydrogenase
Oxidation reduction
Proteasome subunit alpha type
Protein catabolism
Fucoxanthin chl a/c light-harvesting protein
Eukaryotic translation initiation factor 4A
Metabolic process
Predicted protein
Predicted long chain acyl CoA synthetase
Metabolic process
Fucoxanthin chl a/c light-harvesting protein
Adenine nucleotide translocator
Membrane transport
Potentially regulated proteins based on a single peptide at >95% confidence and additional peptides with lower confidence
Gene name
Dihydrodipicolinate reductase
Oxidation reduction
Predicted NonF-related protein; DJ-1/PfpI family
Inorganic pyrophosphatase
Metabolic process
Porin/voltage-dependent anion-selective channel
Membrane transport
Silicon transporter
Membrane transport
Rubisco expression protein
a Accession numbers, gene name and gene ontology information of proteins taken from the UniProtKB/Swiss-Prot database
b Values indicate the number of peptides used for identification and quantification of the proteins.
c Protein ratios in three biological replicates with 0.8 > Regulation factor < 1.2 and P-values <0.05
d Proteins showing the same regulation trend in the DNA microarray experiments
Additional file 4
Peptide fragmentation spectra used for the identification and relative quantification of proteins that have been included in Table
Click here for file
Figure 2
An example of tandem mass spectrometry spectra that exhibited significant iTRAQ ratios between BaP-exposed and control conditions
An example of tandem mass spectrometry spectra that exhibited significant iTRAQ ratios between BaP-exposed and control conditions. (A) The fragmentation spectrum of AIGGVTDGSVR, a peptide fragment from predicted C-5 cytosine-specific DNA methylase (B8BVP1) is shown. The reporter ion signals appear in the low-mass region of the spectrum and are used to determine the relative amount of the protein in
As expected, the quantification of iTRAQ labels 113, 114, 115, 116, 119 and 121 cluster on a principle component analysis (PCA) according to the conditions of exposure (Additional file
Additional file 5
Principle component analysis for the quantification of the iTRAQ labels in the different experiments. The analysis shows a clustering of the proteome analysis from control diatom cultures, labeled with iTRAQ 113, 115 and 119, as well as a clustering of the proteome analysis from the BaP-exposed conditions, labeled with iTRAQ 114, 116 and 121. An additional experiment has been incorporated in the MS/MS analysis, consisting of control and BaP-exposed conditions, labeled with iTRAQ 117 and 118, respectively, but were not considered for the overall quantification analysis. In this experiment, the same starting density of 0.5 million cells/mL was used, but from a culture in the resting phase. We observed that the growth in the diatom cultures was largely reduced from the expected values, and was also observed after the addition of solvent or BaP. We decided to use the cells arising from this experiment with the remaining iTRAQ labels from the 8-plex kit, to see whether under these abnormal cell culture circumstances, we could still observe significant differences between control and exposure conditions. It is evident that both conditions in this experiment sit as outliers on the graph and in addition, they seem to be clustered together. In fact, from the quantification analysis, it was observed that both 117 and 118 labels showed rather consistent values for most of the peptides analyzed, and not in agreement with the experimental conditions, i.e. control or BaP-exposed. It is interesting to note that the reduced cell growth in this culture, greatly affected the outcome of the quantification. Thus, the apparent sensitivity of these cells seemed to be more relevant than the exposure conditions themselves. These findings, although not unexpected, truly support the importance of reproducibility in biomarker research, since any differences in the initial state of the cells has a substantial effect on the biomolecular pool and thus masks any potential differences of expression, either at gene or protein level.
Click here for file
iTRAQ labels 117 and 118 have been used to label untreated and BaP-exposed conditions, respectively on an old diatom culture which was in resting phase. Thus, when we subjected this culture to the same experimental procedure, no significant growth could be observed in the control and BaP-exposed cultures after 24 h. This prevented the measurement of any BaP-induced effect either as growth inhibition or as a difference in expression of the proteome, which underlines the importance of using cell cultures in exponential growth for the purpose of molecular biomarker discovery.
Gene expression analysis of SIT1
The identification of the silicon transporter (SIT1) in our study as a potentially regulated protein after BaP-treatment (Table
Figure 3
Silicon transporter 1 gene regulation
Silicon transporter 1 gene regulation. Bar graph shows the normalized expression mean of the
Since the publication of the entire genome sequence of
Three different proteins involved in photosynthetic processes show a down-regulation in BaP-exposed samples, compared to control conditions. This finding is in agreement with previous reports on a harmful effect of BaP and other PAHs on the photosynthetic activity in plants
DNA methylation
Gene methylation is an ancient property of eukaryotic genomes
Lipid metabolism
Due to its hydrophobic nature, PAHs accumulate in lipidic environments such as membranes and are known to induce oxidative stress and lipid peroxidation
The involvement of the lipid metabolism on the cellular response to BaP exposure is further evidenced on a recent report in human macrophages where it was demonstrated that BaP is able to trigger lipid accumulation in cellular compartments, including endosomes and lysosomes
Silicon uptake and metabolism
One characteristic feature of diatoms is the silicified cell-wall or frustule that surrounds the plasma membrane
Using a quantitative proteomics approach based on iTRAQ labeling of peptides, we could study protein expression changes in diatoms upon exposure to BaP. Despite the low protein identification rate achieved in our studies, we were able to select a set of 19 proteins with changes in expression. Our data shows that the lipid metabolism and biosilicification processes are involved, as observed previously, but also unveils other potential pathways involved in the diatom exposure or in toxicity responses to BaP, such as DNA methylation and photosynthesis. These data, combined with gene expression analysis can help to elucidate pathway/metabolic processes involved in the mode of action of pollutants.
Diatom culture and detection of growth inhibition upon exposure to benzo(a)pyrene
Fresh diatom cultures were inoculated at a start cell density of 0.5 × 106 cells/mL, incubated for 24 h and divided into different flasks for exposure experiments. Diatom cultures were exposed to benzo(a)pyrene (36.45 μg/L) dissolved in methanol while the solvent was added to control cultures at a final concentration of 0.05% (v/v)
Total protein extraction and iTRAQ labeling
For protein extraction, the frozen cell pellet was allowed to thaw on ice, and resuspended with 200 μL of buffer containing 50 mM MOPS, 0.05% Triton X-100, 10 mM DTT and protease inhibitor cocktail (Sigma). Cell lysis was performed using homogenization with a IKA® T10 basic ULTRA-TURRAX, for periods of 2 min on ice, briefly spinning down to remove any foam created by the procedure. Around 5-7 cycles homogenization were necessary for complete homogenization, which was verified by inspection on a microscope. Finally, the samples were sonicated for 30 min on ice and the lysate was centrifuged at 16,000
Each iTRAQ 8-plex reagent (Applied Biosystems) was dissolved in 50 μL isopropanol and added to a total amount of 53 μg of peptide sample. Verification of the samples pH (7.5-8.5) was done and labeling occurred for 2 h at room temperature. Samples were frozen at -80°C and shipped within a day in dry ice to Proteome Factory, Germany for SCX separation and MS/MS analysis.
Peptide SCX separation
Efficiency of labeling from each sample was verified by mass spectrometry before the eight samples were pooled. SCX separation used an Agilent 1100 HPLC system with a PolySULFOETHYL A column (200 mm × 2.1 mm, 5 μm, 200 Å, PolyLC, USA). The iTRAQ labeled sample was dried by speedvac and resuspended in 1.5 mL 0.1% formic acid/25% acetonitrile and the pH adjusted to about pH 2.5. The following separation gradient with buffer A (0.1% formic acid/25% acetonitrile) and buffer B (500 mM KCl/0.1% formic acid/25% acetonitrile) was used at flow rate 200 μl/min (0%B for 10 min; 40%B for 45 min; 70%B for 57.5 min and 100%B for 65 min). 18 SCX fractions were collected in a microtiter plate and dried by Speedvac for subsequent nanoLC-ESI-QStar MS.
nanoLC-QStar MS analysis for protein identification and quantification
Protein identification and quantification of iTRAQ reporter ions were performed using nanoLC-ESI-MS/MS. The MS system consisted of an Agilent 1100 nanoLC system (Agilent, Germany), PicoTip emitter (New Objective, USA) and a Qstar XL MS (ABI, Foster City, USA). Dried SCX peptide fractions were resuspended in 80 μl 0.1% formic acid/1% acetonitrile in MilliQ water. After trapping 40 μl sample and desalting the peptides on enrichment column (Zorbax SB C18, 0.3 mm × 5 mm, Agilent) using 1% acetonitrile, 0.1% formic acid solution for 10 minutes, peptides were separated on Zorbax 300 SB C18, 75 μm × 150 mm column (Agilent, Germany) using an acetonitrile, 0.1% formic acid gradient from 5% to 35% acetonitrile within 110 minutes. MS spectra were automatically taken by Qstar XL according to manufacturer's instrument settings for nanoLS-ESI-MS/MS analyses. Proteins were identified and quantified using MS/MS ion search of Mascot 2.2.06 search engine (Matrix Science, London) with iTRAQ-8plex quantification, using a peptide mass tolerance of 150 ppm, a fragment mass tolerance of 0.4 Da and two missed cleavages. A parallel database search was done using the Paragon algorithm as implemented in Protein Pilot 3.0 software (Applied Biosystems), using the customized search settings. Searches were performed against a protein database containing the entire proteome of
RNA isolation and qRT-PCR
Extraction of total RNA from three biological replicates in both control and BaP-exposed conditions, was performed using the Trizol LS (Invitrogen) protocol, as described previously
Regulation of the expression of the gene
Amplification reactions were performed using the Master Mix 2× (Applied Biosystems), 0.9 mM of reverse and forward primers, 0.1 mM probe, and 2.5 μL of cDNA in a final volume of 25 μL. Samples were loaded in triplicate on 96-well optical reaction plates and qRT-PCR was performed in the ABI Prism 7900HT sequence detection system (Applied Biosystems). Each cycle of the thermal amplification followed the universal protocol according to the manufacturer, i.e. 2 min preheating at 50°C, 10 min at 95°C, followed by 40 cycles with 15 s at 95°C and 1 min at 60°C. Gene expression data from qRT-PCR were evaluated using Q-Gene
BaP: Benzo(a)pyrene; iTRAQ: 8-plex amine-modifying labeling reagents for multiplexed relative and absolute protein quantitation; DTT: dithiotreitol; ESI: electrospray ionization; GAPDH: glyceraldehydes-3-phosphate dehydrogenase; iTRAQ: isobaric tag for relative and absolute quantitation; LC: liquid chromatography; PAHs: polycyclic aromatic hydrocarbons; PCA: principle component analysis; qRT-PCR: quantitative real-time polymerase chain reaction; SCX: strong cation exchange; SIT1: silicon transporter 1; MS/MS: tandem mass spectrometry; TEAB: triethylammonium bicarbonate; TCEP: tris-(2-carboxyethyl)-phosphine.
Authors' contributions
RNC participated in the design of the study, drafted the manuscript and carried out all the experiments and data analysis described except for the SCX separation, the nanoLC-QStar MS analysis and the database search, which have been performed as a service at the Proteome Factory (Germany) who also provided a description for the method. TL participated in the design of the study, designed the primers for qRT-PCR and assisted in drafting the manuscript. All authors read and approved the final manuscript.
The authors, the study and the manuscript preparation have been funded by the European Commission. The EC has approved the publication of the manuscript. We are grateful to Joaquin Pinto Grande and Bruno Paracchini for technical assistance. We acknowledge the good service and help provided with the MS data acquisition and description of the corresponding method by Christian Scheler, at the Proteome Factory (Germany).
The life of diatoms in the world's oceans
Climate, critters, and cetaceans: Cenozoic drivers of the evolution of modern whales
Exploring bioinorganic pattern formation in diatoms. A story of polarized trafficking
Biosilica formation in diatoms: characterization of native silaffin-2 and its role in silica morphogenesis
Biomineralization in diatoms: characterization of novel polyamines associated with silica
A stress-induced protein associated with the girdle band region of the diatom Thalassiosira pseudonana(bacillariophyta)
Whole-genome expression profiling of the marine diatom Thalassiosira pseudonana identifies genes involved in silicon bioprocesses
The genome of the diatom Thalassiosira pseudonana: ecology, evolution, and metabolism
The Phaeodactylum genome reveals the evolutionary history of diatom genomes
Levels and toxicity of polycyclic aromatic hydrocarbons in marine sediments
Gene regulation in the marine diatom Thalassiosira pseudonana upon exposure to polycyclic aromatic hydrocarbons (PAHs)
Gene biomarkers in diatom Thalassiosira pseudonana exposed to polycyclic aromatic hydrocarbons from contaminated marine surface sediments
iTRAQ-based proteomic identification of leucine-rich alpha-2 glycoprotein as a novel inflammatory biomarker in autoimmune diseases
Quantitative proteomics analysis of maternal plasma in Down syndrome pregnancies using isobaric tagging reagent (iTRAQ)
A quantitative proteomic analysis of light adaptation in a globally significant marine cyanobacterium Prochlorococcus marinus MED4
The response of the marine bacterium Sphingopyxis alaskensis to solar radiation assessed by quantitative proteomics
Silica morphogenesis by alternative processing of silaffins in the diatom Thalassiosira pseudonana
Characterization of a modular, cell-surface protein and identification of a new gene family in the diatom Thalassiosira pseudonana
Identification of proteins from a cell wall fraction of the diatom Thalassiosira pseudonana: insights into silica structure formation
Deciphering diatom biochemical pathways via whole-cell proteomics
Photoinduced toxicity of PAHs to the foliar regions of Brassica napus (canola) and Cucumbis sativus (cucumber) in simulated solar radiation
Genome-wide evolutionary analysis of eukaryotic DNA methylation
DNA methylation, methyltransferases, and cancer
The role of mammalian DNA methyltransferases in the regulation of gene expression
The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors
Benzopyrene exposure disrupts DNA methylation and growth dynamics in breast cancer cells
Benzo[a]pyrene diol epoxide suppresses retinoic acid receptor-beta2 expression by recruiting DNA (cytosine-5-)-methyltransferase 3A
Oxidative stress in toxicology: established mammalian and emerging piscine model systems
Structure and regulation of rat long-chain acyl-CoA synthetase
Differential gene expression and biomarkers in zebrafish (Danio rerio) following exposure to produced water components
NPC1 repression contributes to lipid accumulation in human macrophages exposed to environmental aryl hydrocarbons
Do long-chain acyl-CoA synthetases regulate fatty acid entry into synthetic versus degradative pathways?
Application of AFM in understanding biomineral formation in diatoms
Analysis of Thalassiosira pseudonana silicon transporters indicates distinct regulatory levels and transport activity through the cell cycle
Synchronized growth of Thalassiosira pseudonana (Bacillariophyceae) provides novel insights into cell-wall synthesis processes in relation to the cell cycle
Chloroplast genomes of the diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana: comparison with other plastid genomes of the red lineage
Processing of gene expression data generated by quantitative real-time RT-PCR


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subset: commoncrawl


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The platinum database has all the features and content of the gold database but it also offers you the facility to export all fields of information to Excel, including full address details, contacts, polymers processed, markets served/products manufactured, size of products (if relevant) and some machinery information.
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Each week we will explore a common classroom practice. Just keep in mind we are English teachers. This means that we're prone to reciting Whitman and extolling the virtues of Hemingway. It also means that many of the posts will reference specific literature that can be partnered with the suggestions we're offering. It doesn't mean that these ideas are only practical for English teachers. You know, quite well, that teaching requires common practices across the curriculum. We hope that all teachers might be able to use our research and tech posts. We also hope that you, our audience, might be able to find some use within your own classrooms that we can't even begin to imagine. So, with that in mind here is our weekly format.
Monday: We provide an overview of the topic. We discuss what's been done and some of the difficulty we've run into with these standard approaches. We will try to be witty so that you'll a) like us and b) keep checking the blog over the course of the week.
Example topics include but are not limited to:
- Anticipation Guides
- Annotating
- Research & Current Events
- Popular Culture (songs, images, blogs)
Tuesday-Thursday: We offer a suggestion each day (that would add up to three total–look English teachers can do simple math). These suggestions will focus on how to modernize and reinvent the approach which means a focus on small group, classroom participation, popular culture, current events, you name it. We're big on the idea of not creating lessons that are just gimmicks. We know how fun those tech gimmicks can be and we sometimes succumb, too. However, we still want each suggestion to be something that engages students without losing rigor and skills application.
Friday: We offer up a reflection on which approach we like the most and why. Once again we will do our best to be concise. We know you're busy and probably have students who are demanding more education and homework if possible.
Saturday-Sunday: Need a fresh idea? A good story to spark classroom discussion? During the weekend we'll focus on specific websites, stories, blogs, technology, radio programs, etc. that we love with the hope of getting you to test them in your own classroom. We'll even have some guest bloggers from time to time. Anything to keep you interest in updating elements of teaching. We know how hard sometimes it can be to come up with good ideas and we want to help.


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Price-Linked Farm And Spatial Equilibrium Models
The integration of detailed farm supply models with the basic spatial equilibrium model, is outlined. The direct linking of farm linear programming models with the spatial equilibrium model is achieved so that both prices and quantities are endogenous. Both the farm model and the spatial equilibrium model must be specified in primal-dual form to make the linkages. Limited details of the use of such a model in a study of a segment of the grain handling system in New South Wales are presented along with conclusions relating to the pricing of grain handling services.
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Volume (Year): 38 (1994)
Issue (Month): 02 (August)
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References listed on IDEAS
Please report citation or reference errors to , or , if you are the registered author of the cited work, log in to your RePEc Author Service profile, click on "citations" and make appropriate adjustments.:
- John Quiggin, 2012. "Introduction," Introductory Chapters,in: Zombie Economics: How Dead Ideas Still Walk among Us: With a new chapter by the author Princeton University Press.
- Larry J. Martin, 1981. "Quadratic Single And Multi-Commodity Models Of Spatial Equilibrium: A Simplified Exposition," Canadian Journal of Agricultural Economics/Revue canadienne d'agroeconomie, Canadian Agricultural Economics Society/Societe canadienne d'agroeconomie, vol. 29(1), pages 21-48, 02.
- Onal, Hayri & McCarl, Bruce A, 1989. "Aggregation of Heterogeneous Firms in Mathematical Programming Models," European Review of Agricultural Economics, Foundation for the European Review of Agricultural Economics, vol. 16(4), pages 499-513.
- C. Richard Shumway & Anne A. Chang, 1977. "Linear Programming versus Positively Estimated Supply Functions: An Empirical and Methodological Critique," American Journal of Agricultural Economics, Agricultural and Applied Economics Association, vol. 59(2), pages 344-357.
- James G. MacKinnon, 1975. "A Technique for the Solution of Spatial Equilibrium Models," Working Papers 199, Queen's University, Department of Economics.
- T. Takayama & G. G. Judge, 1964. "An Interregional Activity Analysis Model for the Agricultural Sector," American Journal of Agricultural Economics, Agricultural and Applied Economics Association, vol. 46(2), pages 349-365.
- MacAulay, T. Gordon & Batterham, Robert L. & Fisher, Brian S., 1989. "Solution Of Spatial Trading Systems With Concave Cubic Programming," Australian Journal of Agricultural Economics, Australian Agricultural and Resource Economics Society, vol. 33(03), December.
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https://www.iprodev.com/wp-content/uploads/2013/11/screenshot.jpg 300 590 Hemn Chawroka https://iprodev.com/wp-content/uploads/iprodev-logo.svg Hemn Chawroka2013-11-17 10:53:222017-04-06 00:32:36iPresenter (jQuery)
iPresenter (jQuery)
iPresenter is a simple and powerful jQuery plugin that makes it easy to design advanced CSS3 presentations, galleries and sliders.
https://www.iprodev.com/wp-content/uploads/2012/07/iview_screenshot.jpg 300 590 Hemn Chawroka https://iprodev.com/wp-content/uploads/iprodev-logo.svg Hemn Chawroka2012-07-16 17:00:562017-04-06 00:33:44iView Slider (jQuery)
iView Slider (jQuery)
iView is easy to use jQuery image slider with animated captions, responsive layout and HTML Elements like (Video, iFrame) slider. Easily add unlimited number of slides and captions. Use it as image slider, image gallery, banner rotator, banner ads, or even presentation.


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Atomizers in Disguise
At TPE2016, I checked out the Movkin Disguiser, a temperature-control box-mod with several unique properties. The most outstanding feature of this mod is its atomizer cutaway. The cutaway allows a tank atomizer to fit into the dual-battery box while keeping the entire form factor somewhat compact. Check out the video above and keep reading for more details on the Movkin Disguiser.
Movkin Disguiser Features and Form Factor
The Movkin Disguiser's cutaway can accommodate atomizers 25mm or smaller in diameter. It comes with adapters that can be used for atomizers of different heights. There's even an extension tube that allows vapers to use atomizers on top of the box in a traditional manner. The extension tube is useful for low-profile RDAs.
As for the capabilities of the Movkin Disguiser, it's a 150-watt box that offers temperature control with nickel, titanium, stainless steel, and ceramic-tungsten coils. Color wise, it's available in black, white, red, and Tiffany blue.
The form factor of the Movkin Disguiser is somewhat reminiscent of the popular Wismec Reuleaux box mod, but bigger. Vapers with small hands will likely find the ergonomics cumbersome. Even those with medium-sized hands might be put off with the girth of the Movkin Disguiser. I definitely recommend holding one in person in order to see if the shape works for you.
Disguiser? I Hardly Know Her!
I enjoyed the brief time I spent with the Movkin Disguiser. At the very least, it's a unique box mod with a form factor that stands out from the several "me too!" vaping devices on the market. Box mods with atomizer cutaways are popping up all the time, but most of the ones I've seen use a single 18650 or 26650 battery. It's nice to see a dual-battery box with an atomizer cutaway. Hopefully there will be more in the future.
What do you think of the Movkin Disguiser? Are you intrigued by its unique design? Or it too big for what you enjoy? Kindly leave a comment with your thoughts on the mod.


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Edited by Cinemoi - http://www.cinemoi.tv/
http://youtube.com/FashionTV LONDON - Swissdent spokesmodel Hofit Golan gives FashionTV an inside look at her Swissdent Photoshoot with photographer John Oakley for the dental cosmetics brand's latest ad campaign. The photoshoot takes place in One For One Park Lane, a new club, where she is reunited with her favorite photographer, who she's shot campaigns with in the past, including her campaign for Elizabeth Grant. Hofit shows off her beautiful pearly whites with a big smile. Samantha Hosker, Hofit's personal makeup artist and hairstylist on the shoot, offers some beauty tricks like Elizabeth Grant's skincare product The Socializer.
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Last night I took a walk down the road with my daughter-in-law and a friend to check out an Italian restaurant a friend told me about. We were greeted by Chak, the owner who asked which languages we spoke. I said Swahili and he started talking Portuguese. What a character! After finally ordering 3-pizzas, he began telling us his life story in several languages and continued during our dinner. It was better than TV, although I hope I don't have to see a rerun next time I go there, which I fully intend to do because the food was GREAT!
Don't get me wrong about Chak, he's a really interesting and well meaning man who loves to windsurf, internet surf, and travel when he's not in his restaurant. So, if you're near Essaouira and don't mind driving 8-kilometers south to Raswa, you absolutely have to eat at the Chak Cafe if you appreciate good food!
Own or manage this property? Claim your listing for free to respond to reviews, update your profile and much more.


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Gluten Free Pumpkin Spice Cookies
Recipe by Chef Travis W Holla
Oh My!! If you love Pumpkin around the Holidays and really enjoy Gluten Free foods that actually taste Good. Then you will love my recipe.I drizzled a little dark chocolate and white chocolate over then. My Family and everyone at my Church loved them. P.S. Bobs Red Mill makes really good Gluten Free Flour mix Enjoy from Chef Travis Holland
Top Review by Sarah F.
These cookies are amazing!! I used Bob's Red Mill 1:1 Gluten Free Flour, added 1 package of vegan (+GF) chocolate chips, and made sure to cream all the wet ingredients together as you would any cookie recipe. The cookies were moist and fluffy! The family loved them! I also made these with margarine instead of butter on the 2nd time around and they were just as good! Now that I have a fantastic cookie that I can make GF and Kosher Parve (no dairy), I plan on making these often!
Ingredients Nutrition
- 1 (15 ounce) can pumpkin
- 3⁄4 cup brown sugar
- 1 egg
- 1⁄2 cup sugar
- 2 cups gluten-free flour
- 1⁄4 teaspoon salt
- 2 teaspoons baking soda
- 2 1⁄2 teaspoons pumpkin pie spice
- 1⁄4 cup butter, soften
- 1 tablespoon vanilla extract
Directions See How It's Made
- Preheat your oven to 350.
- In one bowl mix all your dry ingredients together.
- In another separate bowl mix all the wet ingredients.
- Combined the wet ingredients into the dry and thoroughly mix together.
- Take a sheet pan and place a piece of parchment paper on it.
- Scoop out the cookie dough with a spoon, making inch long circles with the dough on the pan. You should get 12 on a half sheet pan.
- cook for 10-12 minutes or until lightly browned on top.
- if you would like you can drizzle some melted chocolate on top -- makes it Super Yummy. take some chocolate chips and put them in a glass dish and place it in the microwave. put it in for one minute. Take out and stir. If it is not yet melted, place back in the microwave and repeat the step till it is fully melted and can be whisked smoothly.
- Enjoy! from Chef Travis Holland.


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World of Warcraft's 6.0.2 patch will go live on North American servers tomorrow before arriving on European servers this Wednesday, Blizzard has announced.
Why is that of consequence? Because 6.0.2 is the final patch to be released prior to the launch of the game's fifth expansion Warlords of Draenor, which goes live on November 14. As such, it brings with it a raft of balance and mechanical changes, as well as enhanced character models for most of the playable races. It also marks the start of a new questline to coincide with the invasion of the Iron Horde – the expansion's time-displaced villains – as well as a retuned level-90 version of the expansion's redesigned Upper Blackrock Spire dungeon, which will be raised to level 100 once the expansion launches.
A full list of the changes, including details of changes to long-standing stats, can be found over on the game's official website. Here are some of the highlights:
- Character models for races from the original game Dwarf, Gnome, Human, Night Elf, Orc, Tauren, Troll, Undead, and Draenei have been overhauled with an increase to fidelity and texture resolution; while retaining the core look and feel of the originals.
- Character animations have been updated to have more personality and now support expressive facial expressions. Emotes are now more emotive!
- The Dark Portal in the Blasted Lands has turned blood red. Hundreds of strange-looking orcs are violently pouring into Azeroth, killing everything that stands in their path. Nethergarde and Okril'lon have already fallen, and while the Horde and the Alliance moved as quickly as they could to get reinforcements to their people, they are too late. The Iron Horde invasion has begun.
- A specially revamped five-player version of Upper Blackrock Spire is available to level-90 characters for a limited time, and sets the stage for the coming counterstrike against the Iron Horde.
- Group Finder received an upgrade and expanded to facilitate players trying to create groups for both PvE and PvP content. Browsing or creating a Premade Group can be done via Group Finder (Bound to the I key by default) or the Player vs. Player pane (Bound to the H key by default.)
- The quest log has been integrated into the world map to make it easier to know where to go and what to do.
- Quest items no longer take up space in the inventory and are accessed through the Quest Tracker.
- Tradeskill materials now stack up to 200 (up from 20).
- Added a "Clean up Bags" button to automatically sort items in the character's inventory, moving all empty slots to one area, and automatically sorting items to the correct bag. There's also the option to ignore a bag from cleanup.
- Character stats have been squished into smaller numbers that are easier to understand. It's important to understand that this is not a nerf as enemies' stats have been squished as well.
- All characters now learn some important Major Glyphs automatically as they level up.
For more on Warlords of Draenor, check out Heather's preview from August. And be sure to follow us on twitter and facebook, or subscribe to our RSS feed to be kept in the loop on future World of Warcraft news.


text: Title. I have no idea what I should be doing now to make my character better. I would really appreciate it if someone is able to tell me what steps I should take to work on getting better gear(Please don't give me answers like "farm ancients" or stuff like that because I don't process farming ancients especially when I don't know what I'm actually farming for). Thank you in advance~


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NATO: Six foreign troops killed in suicide attack in Afghanistan
NATO has not confirmed the nationality of the dead
KANDAHAR, Afghanistan — NATO says that six of its soldiers have been killed in a suicide attack near Bagram Airfield in Afghanistan.
Brig. Gen William Shoffner, head of public affairs at NATO's Resolute Support base in the Afghan capital Kabul, said on Monday that three foreign troops were also wounded in the attack.
He says the attack happened at around 1.30 p.m. local time in the vicinity of the base, which is the biggest U.S. military facility in Afghanistan.
The Taliban has claimed responsibility for the attack in an email to The Associated Press.
NATO could not confirm the nationality of the dead, according to policy.
Earlier, an Afghan official put the number of NATO personnel dead at three, with another two wounded. It is the deadliest attack on foreign troops since August.
The week in pictures: 7 days, 15 photos
1 of 15
Heihe, China
Soldiers of China's People's Liberation Army (PLA) jump as part of their winter training in temperature of negative 26 degrees Celsius at China's border with Russia.


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Lead Guitar Breaks for Bluegrass Songs
Arranging Solos for Vocals. Instructional/Guitar/DVD. DVD. Homespun #DVDKAULD21. Published by Homespun (HL.641859).
Item Number: HL.641859
ISBN 1597730467. 5.25x7.5 inches.
Steve Kaufman breaks down lead solos to six classic songs, demonstrating the licks, chord inversions, embellishments, flatpick techniques and other important pointers that will show learning players how to "take it!" 60-MIN. • INCLUDES MUSIC + TAB • EARLY INTERMEDIATE LEVEL.


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I live in Missouri and my back porch faces North. I have the Brinkmann Trailmaster Vertical Smoker, winters can get rough around here and two years ago when I had a different smoker that was protected from the winds it was hard to regulate tempatures. I'm looking for advice what you all think would help.
post #1 of 2
9/27/14 at 12:02pm
Thread Starter


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Breakfast Burritos
Recipe by sloe cooker
Good to freeze and microwave later, when you are pressed for time. Recipe adapted from a Taste of Home cookbook.
Top Review by diner524
Can't go wrong with this recipe for making quick hearty breakfasts on the go!!! I made mine for eating right away so didn't need to thaw/heat. I made a scaled back recipe to serve one. Next time I might even pan fry the tortilla to better melt the cheese and get a crispy coating (I love crunchy taco/chimichangas) but I know my kids would want the soft burrito!! Could see adding other veggies/cheeses and even different meat for these wonderful burritos!! Thanks for sharing the recipe. Made for Fall PAC 2012.
See Discussion:1 Review
Ingredients Nutrition
- 12 slices bacon, diced
- 12 eggs, lightly beaten
- salt and pepper
- 10 flour tortillas, 8 inches
- 1 1⁄2 cups cheddar cheese, shredded
- 1⁄2 cup green onion, thinly sliced
Directions See How It's Made
- In a large skillet, cook bacon until crisp, place cooked bacon on paper towels to drain.
- Reserve 1-2 tablespoons bacon drippings and discard the rest.
- Add eggs, salt and pepper to drippings, cook and stir over medium heat until the eggs are completely set.
- Spoon about 1/4 cup of egg mixture down the center of each tortilla, sprinkle with cheese, onions and cooked diced bacon.
- Fold bottom and sides of each tortilla over filling.
- Wrap each burrito in waxed paper and aluminum foil.
- May freeze for up to 1 month.
- To use frozen burritos:
- Remove foil. Place burritos on a microwave safe plate. Microwave at 60% power for 1 to 2 minutes or until heated through. Allow to sit for 20 seconds before eating.


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Australian star Penny Taylor has guided home the final two free throws as she and Opals teammate Erin Phillips claimed the WNBA championship with the Phoenix Mercury.
The Mercury - coached by another Aussie, former Opals guard Sandy Brondello - beat the Chicago Sky 87-82 on Friday to complete a three-game clean sweep of the series.
Diana Taurasi and Candice Dupree scored 24 points each and DeWanna Bonner added 12 points for the Mercury, who also won titles in 2007 and 2009.
Star centre Brittany Griner sat out following surgery to correct a retina after she was hit in the right eye in Game 2.
Elena Delle Donne scored 23 points, Sylvia Fowles had 20 points and Allie Quigley had 19 for the Sky.
Taurasi hit a tiebreaking short jumper and was fouled with 14.3 seconds left and made the ensuing free throw to put Phoenix up 85-82.
Delle Donne missed a 3-pointer with 12 seconds left, and the Sky then fouled Taylor, who made both free throws with 9.3 seconds left.
Chicago was the first team with a losing record during the regular season to reach the finals.


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I have a Ricoh Aficio SP C232DN printer on my network. It works just fine. I can view the config through the web pages, but i cannot log in as an admin.
The supplier gave me the default password, i have done a full reset and still nothing works. I have googled and tried everything i can find.
I need some ideas from anyone.
8 Replies
May 24, 2012 at 8:12 UTC
We have a different model Ricoh Aficio but the default username is admin and the password is blank. Have you tried that?
May 24, 2012 at 8:16 UTC
May 24, 2012 at 8:31 UTC
check if is available
Default Password List
May 24, 2012 at 8:39 UTC
I already checked the default password list, and the "1111" option doesn't work either.
Willing to try any other suggestions..
May 24, 2012 at 9:06 UTC
Username: Admin (note A is capitalized)
Password: password
May 24, 2012 at 11:01 UTC
Username: Admin (note A is capitalized)
Password: password
+1 to this. Admin with the capital "A" is also required on our Toshiba copiers.
May 31, 2012 at 1:03 UTC
Wondering if this device is corrupt? Still unable to access the admin levels via the built in wen server.
Dec 14, 2013 at 6:10 UTC
1st Post
On typical Ricoh machines the default is all lower case User name: admin PW: left blank. The 232 is NOT a typical Ricoh mfp. There is no user name/pw turned on by default. If you enable the authentication there is no where to put it a user name, just a PW. The PW is left blank unless you have changed the password. On this case there is no supervisor login to reset the PW like other Ricoh models. You have to get into Service Modes and reset the machine to factory default. Note: all settings will be erased so print out a configuration page before doing this. If you have done all these steps and it will not rest I would do a firmware update then replace memory as a last resort.
This discussion has been inactive for over a year.
You may get a better answer to your question by starting a new discussion.


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10 properties in Mollina
Mollina Accommodation
Search 10 hotels in Mollina
Get Secret Prices on selected hotels
These prices aren't available to everyone.
Where to stay in Mollina
Spain   accommodation in Mollina - Hotels.com offers great last-minute Mollina hotels deals and discount room rates. Our hotels in Mollina can easily be booked online. Our fast online search facility allows you to:
- - Find and compare different hotels in Mollina by star rating, amenities, theme, or brand
- - Choose the perfect Mollina hotel and view room rates, hotel details, guest reviews and photos
- - Securely book your hotel room online today!
- - Receive email and text confirmations with reservation details of your online hotel booking


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Locust 4th Hopper / 18
Locust 4th Hopper / 18
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Possibly the very best quality locusts available in the UK. Raised on quality foods, with no expense spared, you can be absolutely sure when buying our locusts that you are giving your animals the nutrition and quality they need.
To get the most from your locusts they are best kept in a warm and dry environment and can be fed on fresh washed dark cabbage and bran.
Each tub contains approximately 18 locusts.
These are 28-32 mm
No customer reviews for the moment.


text: The latest initiative of the Physics for Development group was to launch a program on 3D printing for science with the Cameroon Physical Society in cooperation with Paul Woafo (head of the physics department at Yaoundé 1 University), the founder of the Cameroon Physical Society and its first president. The main goal of this project was to improve the availability of laboratory equipment for use in university, especially for optics accessories. Nowadays taking advantage of the open source availability of 3D design of laboratory equipment it is possible to improve the situation in many universities in which workshops are scarce or not available at all. Through a close cooperation with the Scientific Fab Lab of ICTP (Trieste) a physicist of Yaoundé University Mr Nana Bonaventure was trained for three months and two 3D printer kits with spare parts were sent to him. He was able to build the two 3D printers and to print different objects.


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Prognosis is a company specialized in developing web and mobile applications. Our clients include several companies from USA, Canada and Sweden. We have won their trust by developing high quality application on time and within scheduled budgets. We have been able to produce that high quality software thanks to our main strength - our team. Prognosis is a place that provides opportunities for constant professional development in supportive atmosphere.
We are always looking for people who are eager to take on new challenges, to grow with the team, and to take a part in building next generation of web and mobile applications.
• B.S. or M.S. in Computer Science or equivalent experience
• Very good knowledge of the Android platform
• C++ and C# experience is advantage
• Good working knowledge of English
• Drive and commitment to work in a highly dynamic environment
• 5+ years of software and/or web development experience, especially mobile
• Bonus points for your app being in a major Android App Store (Google Play, Amazon)
• Experience with a wide variety of Android device resolutions and SDK versions preferable.
• Strong technical background required, design and user experience skills a plus
• A bachelors in computer science or equivalent experience
• Experience working with remote data via REST and JSON
• Experience with a distributed version control system (git, maven)
• Play a lead role in the design and development of distributed tablet and mobile applications
• Collaborate closely with other developers, designers to define innovative UI and functionalities optimized for mobile platforms
• Investigate and resolve application issues, bottlenecks and inefficiencies
• Identify and resolve compatibility issues between the wide range of versions and devices
• Work with other mobile teams across the company to utilize and establish best practices for mobile development
• Deliver high quality work on tight schedules
• Strong knowledge of CS fundamentals including data structures, algorithms and complexity analysis
• Experience leading development of mobile applications that have been successfully delivered to customers
• Ability to translate concept designs into user interfaces on Mobile devices
• Knowledge of Android's common software design patterns, including the ones needed to develop connected applications with high-performing UI
• Strong communication, analytical and problem solving skills
• Understanding of system and application performance limits and characteristics
• Deep understanding of the internals of OS frameworks
• Knowledge of memory management and multi-threading
• Experience working on high-volume consumer products
• Committed to test driven development
• Strong sense of ownership, urgency and drive
• Ability to wear many hats, able to change direction quickly, and show eagerness to learn new things.
What we offer
• Competitive pay and generous stock options program
• Flexible work hours
• A small but very diverse team


text:
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Hooper leads a renegade crew of X-tacles hell-bent on destroying Killface and the Haggar Pants Arena.
Wendell visits a friend.
Old friends are reunited.
Someone gets a new pair of pants.
The film Hooper is heavily borrowed from.
Wendell goes undercover. Again.
TM & © Turner Broadcasting System, Inc. A Time Warner Company. All Rights Reserved.
AdultSwim.com is part of Turner Entertainment Digital which is part of the Turner Sports & Entertainment Digital Network.


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New American Lecture
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Almeca Rosson 13 December 2010
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Transcript of New American Lecture
New American Lecture Parts 1. Select a topic 2. Identify important information you want your students to retain. 3. Create a visual organizer. 4. Create a hook. Steps Begin with a hook Students are given a graphic organizer. Teacher begins the press and release. Lecture for 5-10 minutes allow students to make notes in the organizer. Again Periodic thinking reviews Practical Personal Analytical Imaginative A reflection piece to apply and evaluate their learning. Well not that kind of hook. A hook to grab your students. my topic is Water Cycle Sports Figures Equations War funding we wrap it all up with my topic is takes lectures of ole and revamp them into dynamic, interactive opportunities for our students to acquire information. Then Any Questions?Full transcript


text:
Need a Fertility Test in Kennesaw, GA? Simply order online 24/7 and get a fast, affordable Fertility Test at a lab near you (Find More Locations):
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A Fertility Test can help people in Kennesaw, GA check for fertility through various blood and urine markers. A Fertility Test is part of a standard evaluation or as a precursor to in-vitro fertilization.
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Our Fertility Test prices include the doctor's order that you need to get tested in Kennesaw, GA, all lab fees and taxes, and a pdf copy of your results. For current prices, please CLICK HERE.
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For a sample report of a popular Fertility Test panel, please CLICK HERE.
We are delighted to offer a Fertility Test in Kennesaw, GA.
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text:
Brigitte Lajoie
Disclaimer: The views, actions and activities posted by participants on My Ocean are their own and do not necessarily reflect those of Project AWARE Foundation.
- Name
- Brigitte Lajoie
- Location
45.58329, -65.742188 View on map
- Member Type
- My Summary
Hi, I started diving about 6 years ago and it has been a wonderful experience, quite spiritual at times. I recently started my rescue diver course. Also, after watching the splendid movie Shark Water, I was surprised by two aspects : 1)how little the general public, including myself, knows about the essential role that is played by sharks in regard to the maintenance of a healthy global ecosystem, 2) how beautiful they really are.
After watching Shark Water, I changed my steadfast no to shark diving to a definite what are we waiting for? Let's go. Now, I view sharks with such respect and awe. I wish to help more people to become aware of the importance of safeguarding the various species of sharks.
My Ocean Community
My Dive Against Debris Surveys


text:
Carlos Saura began what would become his trilogy with this depiction of a single dress rehearsal for choreographer Antonio Gades's adaptation of poet/playwright Federico García Lorca's tale of passionate revenge. No mere recording of a ballet, Bodas de sangre (Blood Wedding) uses gripping camerawork and heart-pounding rhythmic editing to evoke the experience of moving with the dancers every step of the way.
A French psychologist investigates about famous suicidal women. She finds the case of Antonieta Rivas Mercado, a Mexican writer who died inside Paris' Notre Dame in 1931. To follow the investigation she travels to Mexico to reveal her life.
A married couple and a male friend leave together on a weekend vacation. Stress is brought on by the husband's jealousies. He hopes to play out his fantasies with his friend and wife in order to justify revenge. Geraldine Chaplin, Juan Luis Galiardo, and Fernando Cebrian star in this odd tryst that is heavy on insects and the voyeuristic machinations of the husband's mind.
The greedy relatives of an amnesic and paralytic 45 years old millionaire try him to recover his memory by any means. Surreal look at a family's attempts to shock, bully, frighten, coach, and cajole Antonio Cano into regaining his memory and his management powers after an auto accident. He's middle-aged, wealthy, the leader of a family corporation. After the accident he can't remember his Swiss bank account numbers, can't sign documents, and can't lead his board of directors.
Julian, a middle-aged single doctor, meets his childhood friend Pablo again. The latter is back from Africa and has just married a beautiful young blonde, Elena. Julian falls in love with her and tries to seduce her, but she mockingly pushes him away from her. He then finds that Ana, his nurse, bears a troubling resemblance to Elena. He decides to gradually transform Ana into Elena…
Ángel is an aging professor, a lonely widower without purpose. His lovely, spirited neighbor, Terese, interrupts his suicide attempt when she comes to borrow wine. She invites him for dinner and he meets her lover, Alberto, and their young child. Terese captivates him, he develops a school-boy's crush, and when Alberto invites him to write something for their theatre troupe, called the Stilts, he jumps at the chance. When he's not writing, he spends every waking hour with the Stilts. They take him in. It's not enough for Ángel: he begs Terese to spend time alone with him, and they do become lovers.


text:
HC notice to BBMP on garbage disposal
The High Court on Monday ordered notice to Bruhat Bangalore Mahanagara Palike in connection with a petition by former mayor P R Ramesh, challenging the tender process for disposal of solid waste, both manually and mechanically.
Though the eligibility period for participation in the tender process is three years, the Palike has relaxed it to one year and many parties close to the corporators have been awarded tenders, he said.
The Division Bench comprising Chief Justice Vikramajit Sen and Justice A S Bopanna refused to stay the procedure, but ordered notice to the Palike and the Urban Development Department.


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Getting your groove on has some superstar health benefits!
1. Dance as an aerobic exercise boosts self-esteem & brain power.
2. It reduces your adrenaline levels, relaxing you.
3. Just one hour of dancing can burn 250 to 500+ calories!
Related article: 5 Easy Ways to Burn 100 Calories Fast


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MMLIS offers a broad array of investment products, including:
In addition to these, Investment Advisor Representatives affiliated with MML Investors Services' Corporate RIA (CRIA) have access to a full array of advisory platforms and private money management services.
Securities products are typically offered by prospectus or offering memorandum. An investor should carefully consider the investment objectives, risks and charges and expenses of the product before investing. The prospectus or offering memorandum contains this and other information about the product and may be obtained from your registered representative. Please read the prospectus or offering memorandum carefully before investing or sending money.
Direct business
Brokerage business
CRIA business
Bank Deposit Sweep program (FDIC)
Through the BDSP, the brokerage account cash balance (from securities transactions, dividend and interest payments, and other activities) is automatically deposited or swept into interest-bearing deposit accounts at one or more FDIC-insured depository institutions ("Program Banks").
- Interest Rates, Program Banks and List of Eligible Accounts
*Subject to availability.
Order Routing Report | Privacy Policy
| Terms of Use
Massachusetts Mutual Life Insurance Company (MassMutual), 1295 State Street, Springfield, Massachusetts 01111-0001.
Securities, investment advisory and financial planning services offered through MML Investors Services, 1295 State Street, Springfield MA 01111-0001, (800) 542-6767. Member FINRA - www.finra.org / SIPC - www.sipc.org.
Copyright MML Investors Services. All rights reserved.


text:
Inattention in Individual Expectations
Consumption-Wealth Ratio and Expected Stock Returns: Evidence from Panel Data on G7 Countries
Microfounded Forecasting
Testing Consumption Optimality using Aggregate Data
Ensaios Econômicos da EPGE, 2014.
Forecasting Multivariate Time Series under Present-Value-Model Short-and Long-run Co-movement Restrictions.
Estimating Brazilian Monthly GDP: a State-Space Approach.
A Note on the Forward- and the Equity-Premium Puzzles: Two Symptoms of the Same Illness?
Non-Durable Consumption and Real-Estate Prices in Brazil: Panel-Data Analysis at the State Level.
Constructing Common-Factor Portfolios.
Ensaios Econômicos da EPGE, 731, 2012.
A Stochastic discount factor approach to asset pricing using panel data asymptotics.
An Econometric Contribution to the Intertemporal Approach of the Current Account.


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Fluent and CFX benchmark
Hi CFX users,
I am interested in knowing if CFX and Fluent has been benchmarked for heat transfer problems. Which software provides better solution and provides higher productivity (ease of use, solver and parametric studies)?
I am interested in heat transfer in a rotating equipment with convection from fan air flow.
Thanks in advance for your suggestions.
Thanks, Ashutosh
Re: Fluent and CFX benchmark
hi A.Joshi!! I am working on CFX exactly for th same issues. I think that CFX is a very good solution to study rotating system with convection from fan air flow. It provides very good productivity even if its very difficult to make local studies. I studied air flow cooling of power generators.
Let me know if u have any questions about CFX for the heat transfert study in rotating equipments.
Re: Fluent and CFX benchmark
Thanks Alex, How do you model Fan in CFX? Represent as momentum source or fan object??
Thanks, Ashutosh
Re: Fluent and CFX benchmark
Hi Ashutosh, u can model it as momentum source if you know the curve "Q vs pressure". u have to know that the "pre rotation"( = shirling in front of the fan's air inlet) effect are very important. In fact, it could midify the fan curve. SO, to take account for the shirling effect, u have to represent the fan in yor 3D model.
Are you working on power generator model? For a company? Do u use CFX CFD software or Fluent?
|All times are GMT -4. The time now is 18:24.


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This property is no longer available.Search for Available Property.
10 acres in Otsego County, Michigan
Beautiful 10 acre property with 1375 sq. ft. ranch home and pole barn only 2 miles from Meijer and 5 miles from I-75. The land is about half cleared and half wooded, so would support your choice of many different hobbies. Metal roof on the home and appliances are included (many are newer). Need more than 2 bedrooms? There's plenty of space to add on here.
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Reviews (0)
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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
My survey responses are all accumulating in one email thread.Asked by grandtrunk on March 21, 2016 at 04:20 PM
All of the responses to my survey are attached in one email chain. How do I make sure they come in separately?
This depends on the email client / webmail that you use, but in general each allows you to break threads and show all emails as a single email instead.
Alternatively, you could change your forms title so that it shows a unique entry, like users full name, or submission ID, etc (depending what serves you best).
For example:
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a new email would have different ending so it should not become the same thread, like:
New Submission has been made by GrandTrunk
Hope that this helps.
The following guide will assist you in the steps on changing the email subject: How to place form title in the Subject Line of form notification?


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Months ago I found a list of the top ten cameras that are used on Flickr but it looks like the stats have changed a little bit. This time the charts are coming directly from Flickr with the Canon EOS Digital Rebel XT at the top of the list by a mile. What is even more interesting is that they also break-down the stats to show the most popular of point & shoot cameras and cameraphones.
The most used point & shoot camera on Flickr is the Canon PowerShot SD400 which is a 5MP camera. It is quickly losing some ground but its successor, the PowerShot SD450, is coming up right behind it and looks like it will be replacing it as the most popular camera. At any rate, Canon has earned the top-5 spots on the list of point & shoot cameras.
The cameraphones, however, are a slightly different story and aren't necessarily accurate. The page that lists the popular cameraphones says that they are often unable to identify which camera snapped a photo that was taken by a phone. For that reason some of the results may not be as accurate but the sleek Sony Ericsson K750i takes first place. From the looks of it Sony won't be in first much longer because two models in the Nokia N series have almost surpassed it.
I love seeing stats like these because they help you see what photo enthusiasts are actually buying and using for their own cameras (and phones for that matter). That way when you go out to get your next camera, especially with the holiday season around the corner, you'll have a good idea as to what camera is the most popular…instead of relying on the sales person to tell you that the most expensive one is the best.


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Monday, September 26, 2005
Amritsar : The epilouge
Covering the Jalianwala Bagh, Goldelden Temple and the Wgah Border in a day was hard for us tender mortals. We were totally drained by the heat and humidity of day 1 and so started late on day 2. Climate was no better the second day. We were to cover local spots and do shopping. Our train would leave at 9 in the night and so we manged to bargain for half day room rent at the hotel. We had in mind to visit the Durgiana Temple and the Ram Tirth. As the Durgiana Temple is withing the city, we started for that.
Durgiana Temple :
It was also the Janmastami day and thus the trip also served the ritual of visiting a temple for paying obeisance. This temple was started by efforts of Pandit Madan Mohan Malaviya. It is built in the Golden Temple style with an inverted lotus dome. The dome here is probably of brass, not gold. Like the Golden Temple, this one also stands in the center of a large lake. Inside there are idols of Krishna and Ram. There is nothing to shower praise on the place. Being a regular feature of Hindu temples, I saw the first of the beggars in Amritsar. From the market outside we bought small Krishna idols as souvenirs.
Ram Tirth:
This is supposed to be the place where Valmiki had his ashram and where Sita gave birth to Luv and Kush after being abandoned by Lord Ram. This is one of the places in the sightseeing list of Amritsar. It was about 15 km from the city and half of the road was kutcha and in bad shape. It was dusty. Do not even think of going there in auto. A shock was waiting for us to unfold after this hard travel. It was a sight of apathy all around. There is a big tank only half of which was filled with water. We could see the old temple of Vakmiki Kutir in the distance on the other side of the tank. People living around have built modern temples to help tourist get over their disappointment. It is upto you whether you go to this temple and shell out offerings in reward for those running them. We avoided barring one or two. The sun was blazing down hard. Braving the sun, we managed to reach the main temple. Condition of the temple will make you wonder if it really is the place! It is managed by Sikhs and there co-exists a gurudwara as well. In fact, all Sikh gurus call themselves descendents of either Luv or Kush. It was quite tough to travel bare foot on the boiling tarmac of the temple complex. We still managed a complete coverage of the monuments there. There is well nearby which people believe is there since the days of Ramayana (look at the photo above). Its water is considered sacred. It looked old, but wondered if it is there from the days of Ramayana. We were told that water from this well was taken for purifying the Amrit Sarover in the Golden Temple when it was being dug. We also saw the place where Luv – Kush supposedly stopped the Ashwamedha horse of Lord Rama. There is another temple on the bank of the tank where Sita is said to have been cremated. You need to be too religious to believe all these, not like me. I am not heretic, but just skeptic.
I felt like doing a short cut to avoid the sun. But as advised completed the parikrama of the tank. We were told by the cab driver that this poor condition of the place is a result of neglect and corruption. Nothing is done by the Government or the temple management to improve attractiveness or beautification of the place. My suggestion is to avoid it. You will feel like wasting your time going there. For me – now I have been to both the places where Ramayana and Mahabharata had supposedly been written. (Mahabharata was written by Ved Vyas in a place called Vyas Ponthi located in Mana village near Badrinath. We went there in our Badrinath trip.)
Travelling to Amritsar:
The no. 329 Dehradun – Amritsar Lahori Passenger that leaves Dehradun daily at 7-10 pm is best avoided as it takes 5 hours to reach Saharanpur. So we had decided to catch the 2903 Golden Temple Mail from Saharanpur at midnight. Travelling by taxi to Saharnpur takes about one and half hour. (for God's sake, hire a taxi from the taxi stand, not from the MK Travels in the Rajinder Nagar market near ONGC Colony). The train had dropped us at Amritsar at 6:30 in the morning, almost on time. While coming back we took the no. 330 Lahori Passenger that leaves Amritsar at 9 pm and dropped us at Saharanpur at 5:30 am next morning. The Golden Temple Mail leaves at 9:30 pm, but it drops you at Saharanpur at the uncomfortable hours of 3:30 in the morning. So, it is for those going to Delhi and beyond. Get off here as Lahori passenger takes almost 7 hours to reach Dehradun from Saharanpur. Buses to Dehradun are easily available from just outside the station anytime. We were home by 8 am.
We then moved into a hotel named City Heart located hardy 50 mtrs from the Jalianwala Bagh memorial. It was quite hot and humid. We got AC rooms for 550 which was not a bad bargain. Hotels in this area charges higher as they are very close to the Golden Temple. Hotels further away are cheaper, but one should prefer to stay close to the Golden Temple. I saw many hotels near the Sangam cinema. The Golden Temple also runs Sarais, a kind of dharamsalas, with a very nominal charge of Rs.50/- for non AC and Rs.200/- for AC rooms. One can try for accommodation there as well. After all, it is just a matter of spending one night.
Local Traveling:
Autos are quite cheap as compared to other places. The cab that dropped us at hotel from station took just 50 bucks. For a complete day hiring of a taxi that includes local sightseeing and Wagah Border costs Rs.650/-. We hired a taxi for the trip to Wagah Border only for Rs.450/-. Next day we hired a taxi again for local sightseeing plus Ram Tirth about 15 kms away for 300/-. You can also hire an auto for local sightseeing. But avoid hiring an auto for going to Wagah Border, for traveling 60 km on auto is no fun, and also to Ram Tirth, as the road is bad and dusty. Ricksaws normally charges 5 bucks for a short distance ply and 10 bucks for little longer ply.
If you avoid the Durgiana temple and ram Tirth, then Amritsar becomes just one-day destination. You can even catch the Golden temple mail at 9:35 pm to come back. However, you may like to do some shopping in Amritsar. Everyone will tell you that clothes are cheap here. But we found not much difference between Dehradun and Amritsar price. On the way back from Wagah, one can buy cloths from the OCM factory retail shop that is by the main road. There is a market just outside the Golden Temple for souvenir shopping. I bought a sword, the symbol of courage of the Sikhs as a souvenir.
That's all folks. Here are few numbers that may be of help :
Hotel City Heart : 2292282, 2554511, 2549301
posted by Rupankar Mahanta at 2:18 PM
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Apple says it has dealt with the rogue server that was causing apps to crash these days
0. phoneArena posted on 06 Jul 2012, 07:54
Apple issued a statement that the app installation problems that were occurring in the last few days have been resolved. The issue was raised by the Instapaper creator, when he got an influx of emails prompting him that his apps is crashing each time users are trying to run it after the latest update. The issue was reported happening with many other apps as well, even after delete and a clean install...
This is a discussion for a news. To read the whole news, click here
1. zhypher_23 (Posts: 195; Member since: 04 Jun 2012)
See, Apple ain't Perfect. Makes me sick people saying Apple has everything.
5. ZayZay (Posts: 571; Member since: 26 Feb 2011)
Wait wait wait wait wait. Apple admitted to a problem? This would be the only time if someone posted the "First" crap (first comment) and it would truly make sense.
Way to blow it zhypher_23
2. Obie-Wan (Posts: 103; Member since: 24 May 2012)
I thought iPhone apps never crashes !!! XD
3. Droid_X_Doug (Posts: 5993; Member since: 22 Dec 2010)
I am now wondering what exactly happened to the server that went rogue? Terminated with extreme prejudice? Or?


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Termas de Rio Hondo Vacation Packages
Why not spend some time exploring the area? Start with a trip to the the Gen. San Martin Artisan and Cultural Center.
For an authentic Santiago del Estero (province) experience, make sure you venture beyond Termas de Rio Hondo and explore the cultural and scenic gems that the region offers. Santiago del Estero, is 40 miles (64 kilometers) to the northwest, while Tucuman is located 50 miles (80 kilometers) to the southeast.
While you are in Termas de Rio Hondo, make a day trip to Santiago del Estero. Approximately 40 miles (64 kilometers) away, this regional city is where you can find Casa de Gobierno, Santiago del Estero Cathedral and Plaza Sarmiento. Teatro 25 de Mayo and Plaza Libertad are also good spots for checking out while you are in Santiago del Estero.
Traveling to a place encompasses so much more than just the scenery, it's also about the words, stories and ideas of a region. Your stay in Santiago del Estero will become all the more rewarding if you take the time to learn a few useful words and phrases in Spanish. "¿Querría bailar conmigo?" is the Spanish way to say "Would you like to dance with me?" Or try "Este caballero pagará por todo" (This gentleman will pay for everything).
You'll be reluctant to say "Adiós" (farewell) to this interesting place.


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office assitant
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• banking
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• accounts payable
• assisting with travel
• website updates
Our office manager will have strong computer skills and strong critical thinking skills. Our office manager will work extremely well independently, strive to support our office objectives, and be very compatible with our sales team.
If interested in learning more, please send a detailed letter and your resume ( alexiswashington629@[url removed, login to view] ) Thanks


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Fake Wine, Batteries Among Goods Seized in Busts
They're not only fake, but they can also be dangerous to your health.
Can You Tell Which Is the Counterfeit Website?
Counterfeiters are now duping consumers with copycat websites of real companies.
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- Description
This simple dining table and chair set will give your casual dining room a charming style that you will love. The smooth rectangular table top has rounded edges, above pretty turned legs. The simple chairs feature elegant lyre shaped backs, with elegant curves. A contoured wooden seat and pretty turned legs complete each simple but stylish chair. Add this lovely dining set to your home for a casual and comforting space where friends and family will love to gather.Includes
1 Dining Table and 4 Side ChairsDimensions
Table, 47.5"L x 29.5"W x 29.5"H
Chair, 15.5"L x 17.25"W x 36.5"H
More from this collection: Damen
- Additional Information
Additional Information
SKU 12601791 GTIN N/A Collection Damen Assemble Length 47.5 Assemble Width 29.5 Assemble Height 29.5 Assembly Required Yes Material Solid Wood Finish White Sizes 5-Piece Sets Styles Casual Shapes N/A Shipping Time 1-2 Weeks Free Shipping No
- Reviews
Disclaimer: Pictures displayed may include optional products not included in the price. Computer monitors often display color variations. Color swatches are shown if alternative colors are available. Please call us or email us if you have any questions!


text:
What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
How many submissions per hour does the $9.95 plan allow?Asked by WTTI on May 12, 2014 at 03:37 PM
How many submissions per hour does the $9.95 plan allow?
From what I know we don't put limits on the submissions per hour. It only consumes the limit of your package plan so for PREMIUM it has 1000 submissions / month if you consume this.
Now if you want to control the submissions then the "Form Limits" feature is the one that you need but from what I know Jotform doesn't have hourly submission limit.
Ok. I had read on the forum that the free service is limited to 2 per hour. Is that not correct?
Sent from my iPhone
That limit is for invitation mails or if you forward an email and not the actual submission. Actual submissions are initiated by the user who views the form.


text:
'Zoe Classic' Pump
Details & Care
Go-to style and superior comfort combine in an essential pump designed with orthotic arch support and memory foam cushioning for a soft, smooth ride.
- Aegis® antimicrobial lining.
- Approx. heel height: 2 1/2".
- Lynco Orthotic footbed promotes support, balance and alignment.
- Leather upper/polyurethane lining/rubber sole.
- By Aetrex; imported.
- Women's Shoes.
- Item #560547


text:
If u have a guy that likes to drop a little gift off from time to time. Is it flowers or chocolates u get and what would u rather have?
Guys what do u get if anything as a random surprise gift for your SO?
Flowers or chocolates? Which do you prefer as a surprise gift girls? Guys what do u usually get them?
If u have a guy that likes to drop a little gift off from time to time. Is it flowers or chocolates u get and what would u rather have?
Have an opinion?
What Girls Said 8
You can't eat flowers.
Hmmmmm true I'd want chocolate as a female or male lol
you know what, i think flowers are great but a lot of money LOL i'm one of those "get me one rose and i'll be happy rest of the week" kinda person. i rather you surprise me with a date somewhere we haven't been before or get me something interesting, not like chocolates or flowers.
definitely, definitely not saying i don't appreciate them, i for sure do. but there's cheaper stuff you can get that'll mean more to me.00|00|0
I like that one
Chocolates , yumm00|00|0
Neither, I'd much rather be surprised to go see a movie or go to an amusement park or carnival... I've never been on a romantic date.. So I think it's be thoughtful and fun :-)00|00|0
Both.. they go well together :)00|00|0
I would like a bag of white cheddar Smart Food popcorn please :-)00|00|0
I've never been given flowers before :(00|00|0
Awe (FLOWERS) for you Kay :-)
I would want white chocolates flowers will die bt regardless I would want the gift to be thoughtful with me in mind not something cliche some people get when there rushing to just buy anything.00|00|0
What Guys Said 1
I actually gift both, I've always thought they come hand in hand and should go together.00|00|0
Learn more
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You cannot undo this action. The opinion owner is going to be notified and earn 7 XPER points.