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English Teacher - January 2017
- Recruiter
- Randstad Education Group
- Location
- Wrexham, Wales
- Salary
- £24999 - £28999 per annum
- Posted
- 18 Nov 2016
- Closes
- 16 Dec 2016
- Ref
- 18111603
- Contact
- Christopher Verburg
- Sectors
- Education & Teaching, Secondary
- Contract Type
- Permanent
- Hours
- Full Time
English Teacher - January 2017
A secondary school in Wrexham are looking for a strong English teacher to join them on their journey to excellence.
As well as positive students and talented staff, they offer many advantages to the successful teacher.
The English department is part of the Communications Faculty which also includes English as an Additional Language and literacy. All students will take GCSE English language and literature.
The departments are well resourced with interactive Promethean whiteboards and appropriate support staff. The new build will include subject-specific facilities.
The newly appointed member of staff will be an excellent teacher with a passion and flair for English teaching. You will be an ambitious, committed and hardworking team player who will support their faculty and the Leadership Team in continuing to improve the school.
We are proud to support a diverse portfolio of schools within the North Wales area. These schools range in size, catchment, religious background and ethos. Within these schools our teachers support pupils across Key Stage 3, 4 and 5. We understand that every school and child is different so we carefully match our teachers with the schools and children to which they work with best.
Randstad Education is the UK market leader. We have been helping teachers and support staff to find temporary and permanent education jobs for nearly twenty years. Working with over 2,500 schools each week we can find the right match for your skills.
In reward for your hard work and commitment we provide competitive rates of pay with no hidden deductions, a refer a friend scheme, teacher of the term awards, access to Randstad Education's online CPD portfolio, long-term and permanent job opportunities, a dedicated consultant with a 24hr on call facility and an honest and trustworthy approach that matches you to the right school.
Please apply online to be considered for this position.
All candidates will be subject to Randstad Education's child safety standards and UK eligibility checks


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Home > Product Categories > Pro-Video > Video Recording & Acquisition > Camcorders > HVRS270U
Item #: HVRS270U MFG #: HVRS270U
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The HVR-S270U offers videographers a traditional "on-the-shoulder" feel with the benefits of increased camera stability and longer recording time of up to 4.5 hours with standard size cassettes.
Standard-Size Cassette CompatibilityA standard-size cassette tape provides approximately 4.5 hours of HDV/DV recording or approximately 3 hours of DVCAM recording. Of course, recording on a miniDV tape is still an option.
"Dual Finder" New LCD Position on EVF UnitThe new LCD position on the EVF (Electric View Finder) unit is currently only available on Sony's professional HDV shoulder-mount camcorders.The HVR-S270U camcorder features a new configuration of a 3.2-inch type XtraFine LCD monitor (approximately 921,000 dots) and an XtraFine EVF (approximately 1,227,000 dots, 852x3[RGB]x480)*. The LCD is located on the EVF unit. This unique layout enables the camera operator to perform both traditional EVF monitoring and LCD monitoring, while the camcorder is held on the shoulder. The 180-degree tilt mechanism allows for LCD monitoring when the camcorder is held in a high or low-angle position, and the 270-degree swivel mechanism will allow LCD monitoring from the front, right, or even left of the camcorder so that a reporter or a director can monitor what is being captured by the camcorder.
HD/SD-SDI & Other Output ConnectorsAn HD/SD-SDI output connector provides embedded audio and TC data and can, for example, be linked up to an XDCAM HD deck. BNC connectors provide secure cabling connections.
Four-Channel Audio InputFour-channel audio recording is realized in HDV and DVCAM thanks to four XLR audio input connectors - two at the front, and two at the back.BP Series Battery CompatibleV-lock professional BP batteries are another great feature in common with broadcast camcorders.
Smooth Slow RecAs with the HVR-Z7U, the HVR-S270U camcorder enables slow-motion playback by capturing images four times faster than the normal field rate (240 fields/s).
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Butternut squash is a warm season vegetable grown in home gardens across the U.S. This winter squash develops a hard outer shell encasing delicate orange fruit as it matures. If harvested after the fruit matures and seeds have developed it can be eaten fresh or stored for months in a cool dry place. Harvesting takes place in the late fall before the first hard frost.
Monitor butternut squash a week or two before the expected maturity date. Although there is some variation between cultivars, butternut squash generally matures in 110 to 120 days from planting or 45 to 55 days from blooming.
Color Changes
Look for a deepening in color. Butternut squash ranges from cream to yellow and darkens to a tan when ripe. Check the outer shell, ripe winter squash will resist a scratch from your fingernail and vines will begin to die back when the squash is mature.
Harvest butternut squash on a clear dry day when the squash are dry. Cut the squash from the vine, leaving a two to four inch stem attached to the squash to prevent the introduction of disease or bacteria that may cause decay. Store in a cool dry area with temperatures between 50 and 55 degrees.
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Grow butternut squash in sunny spots and fertile, freely draining soil. Water regularly in dry weather and apply nitrogen fertilizer.
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Pick acorn squash when the skins are dull and tough, which is usually 80 to 100 days after sowing. An orange patch...


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Prime Minister, step in to save the Bight!
With your help, we've just booted BP out—but now it's time to protect the Great Australian Bight for good.
BP may be gone, but it isn't the only oil giant with a lease to explore the Bight.
Our next big goal is to ensure this unique and vital stretch of ocean gets the protection it deserves; that's why we're calling on the Prime Minister Malcolm Turnbull to rescind ALL oil and gas exploration leases in the pristine Bight.
The Bight is one of our most diverse marine sanctuaries, home to over 36 species of whales and dolphins, and a vital breeding ground for the endangered southern right whale. It's no place to drill for oil.
Sign the petition and join the groundswell against Big Oil
Linda Irwin, the Kangaroo Island local pictured above, knows what's at risk better than most. If a spill were to occur in the Bight, Kangaroo Island's coastline would be covered in oil.
Will you support Linda by joining us for the next step on our journey?
Now that we've won once, we know we can win again. So join the growing sea of voices committed to protecting this vital and diverse marine environment for generations to come.


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|Message view
|« Date » · « Thread »
|« Date » · « Thread »
|"Mark H. Wood" <mw...@IUPUI.Edu>
|Re: [OT] Re: Securing Tomcat Applications from Reverse Engineering
|Fri, 22 Jan 2010 15:17:35 GMT
On Thu, Jan 21, 2010 at 03:02:41PM +0000, Peter Crowther wrote: > 2010/1/21 Mark H. Wood <email@example.com> > > > Reverse engineering is not a technical problem; it is a legal > > problem. You need a lawyer, not a program. > > > > Mmm, yes and no. Burglary is also a legal problem, but I have locks (on / > around the things I want to keep, of a cost and quality appropriate to my > expected loss) as well as being able to engage a lawyer if required. The analogy is imprecise. If you lease a house to someone, you have no feasible technical means to control who enters your house -- the lessee possesses a key and can let in anyone he pleases. But you could write a lease which constrains the set of people lessee is permitted to allow in. (Dunno why, but you could.) The house would be useless to lessee without a key. Similarly a program, distributed to a user, would be useless unless an intelligible version can be loaded or derived by the user's equipment. But if the user's equipment can load or derive an intelligible version of the program, the program can be reverse-engineered. That's why software licenses almost always contain specific language about reverse engineering. In both cases the owner has *necessarily* given up technical control of the property, and can only exert control through legal means. You can't stop people abusing property that you hand over to them, but you may be able to punish them if they do. -- Mark H. Wood, Lead System Programmer firstname.lastname@example.com Friends don't let friends publish revisable-form documents.
|View raw message


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Product Description
The covered stock pot is for boiling, blanching, canning, deep-frying, and poaching. The steamer insert is ideal for pasta, vegetables, and fish. 25 year warranty. Perfect for cooking soups, pasta, stews and steaming vegetables. Cooking with steam retains more nutrients in your food. Glass lid allows you to monitor your cooking without removing the lid and loosing heat. Cast, stainless steel handles are secured with rivets. Polished stainless steel means durability, beauty and resilience.
· Encapsulated bottom
· Rolled edge for easy pouring
· Stainless steel construction
· Suitable for all cook tops including induction
· Oven and dishwasher safe


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Live in NYC. As part of book research on 1877 violence in Scranton and Reading, I'm planning a week's trip through mine country (my second -- first was in July 2001; only fitting the second should be in winter, eh?). Must spend a day or two in Scranton and in Pottsville, for specific research needs. Otherwise, would like to view Sunbury, Shamokin and if I can locate it, Mt. Laffee. specifically the site of Gowen's mining venture there before he turned into the "Napoleon of American railroading" -- that is, when he was just one more bankrupt leaseholder of a mine property. (He's no friend of mine, I just hope to unravel some aspects of his particular monomania / megalomania, and the land everywhere whispers tales otherwise untellable, if only we listen....).
Any leads on locating any Mt Laffee mine(s) will be hugely appreciated; as well as any alternate suggestions of sites to take in during the limited time I have available.
Thanks to any and all in advance.
-- Doug


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Question: The following data are the selling prices in thousands of
The following data are the selling prices, in thousands of dollars, of random samples of existing homes in three different regions of the United States. These data can also be found in the Excel file Midwest.xlsx.
View Solution:
Perform a hypothesis test using α = 0.05 to determine if there is a difference in the median selling price of existing homes in these three regions of the country.
View Solution:
Answer to relevant QuestionsBecause of the popularity of National Football League (NFL) games, tickets can be difficult to acquire if you are not a season ticket holder. Many people buy NFL tickets on the secondary market when the owners of season ...Using α = 0.05, perform a hypothesis test with the data from Problem 18.35 to determine if the population rank correlation coefficient is less than zero. From Problem First time home buyers are an important segment of the housing market because this group allows current homeowners to sell their home and purchase another. In 2012, the median age of first time homebuyers was 31 years old. ...According to student loan provider Sallie Mae, the median amount of money borrowed by a student for college during the 2011– 2012 academic year was $ 7,874. Suppose Sallie Mae would like to determine if there was an ...H& R Block, a tax preparation company, believes that the median tax refund for 2014 was greater than $ 3,000. To test this belief, the following random sample of 2014 re-funds was collected. These data can also be found in ...
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Orchestra Miami's next program in the Family Fun Concert series offers five different programs to introduce kids to the arts through live music. This diverse series features symphonic music, opera, chamber music, folk music and more.
The performance lasts approximately one hour and is appropriate for children of all ages, and juice and cookies will be served after the performance. All performances are at Pinecrest Gardens, 11000 SW 57 Ave., Pinecrest, 33156. Free on-site parking.
Single Tickets for the concerts are $10 for children (ages 3- 17); $15 for adults, $12 for Seniors (65 & up). Subscription prices for the five concerts are $60 for Adults, $50 for Seniors (65 & up) and $40 for children (ages 3- 17) and a Family Pack (2 adults + 2 Children) is $195. Subscribers receive benefits, such as priority seating and study guides/program notes emailed in advance. Tickets are now on sale. To purchase tickets, please visit www.orchestramiami.org or call (305) 274 -2103.
Here's the schedule:
Nov. 4, 2016 at 8 pm: Tyrannosaurus Sue: A Cretaceous Concerto: What ferocious former meat eater is 40 feet long, 12 feet high and weighs more than 10 tons? It's Tyrannosaurus Sue- the largest and best preserved existing T-Rex fossil in the world! Through the magic of music, we travel back 67 million years and discover the life and times of Tyrannosaurus Sue as she competes with a tricky Troodon, chases a Parasaurolophus, and battles with a menacing Triceratops! Performed by the Chamber Ensemble of Orchestra Miami, conducted by Elaine Rinaldi, with narration in English and Spanish.
Jan. 6, 2017 at 8 pm: Visions of America: Teach kids about the power of the wind – both in terms of music and for the planet. Zephyronia is a comic musical parable by composer Bruce Adolphe, with story by Louis A. Gikow, about wind energy and the importance of saving our world with cooperation and understanding; set on the planet of ZEPHYRONIA. Flora and Flutter save the day and make Zephyronia a better place through planet-friendly energy.
March 3, 2017 at 8 pm: Little Red Riding Hood: What happens when Little Red stops to talk to a stranger in the woods? Find out as Orchestra Miami presents Little Red Riding Hood, an opera by Seymour Barab. Performed by three professional opera singers in English, with Orchestra Miami, Elaine Rinaldi conducting.
March 31, 2017 at 8 pm: Matthew Sabatella and the Rambling String Band: With vocals, guitar, banjo, fiddle, mandolin, and bass fiddle, Matthew Sabatella and the Rambling String Band bring to life music that is woven into the fabric of the United States: traditional folk songs, fiddle tunes, old-time country, bluegrass, Appalachian music, ragtime, blues, spirituals, railroad and cowboy songs, work songs, sea shanties, reels, breakdowns, ballads, and more. A popular favorite, your kids will have a rollicking, foot-stomping good old time.


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If you have ever dreamed of learning from and hanging out with master musicians Adrian Belew, Tony Levin, and Pat Mastelotto here is your chance. Adrian, Tony, and Pat will host a music camp at the Full Moon Resort, a … more »
Join Tony Levin at Three of a Perfect Pair Camp Coming This AugustFebruary 7, 2011
Stanley Clarke Joins The Ampeg RosterDecember 10, 2010
Ampeg is proud to announce their latest and greatest signing — bass icon Stanley Clarke. As a genuine pioneer, Stanley has been playing, recording, composing and innovating for more than four decades. Challenging most of the traditional notions of bass … more »
Artist Spotlight – Joe Lester of IntronautJuly 28, 2010
We recently caught up with Ampeg endorser, Joe Lester. He plays with the experimental hardcore band, Intronaut. They are currently on a North American touring, supporting prog rock legends, Cynic. Joe is a seasoned bassist known for his innovative … more »
Bob Glaub: On the Road with the AmpegMarch 2, 2010
Bob Glaub has stood alongside some of the music industry's most enduring legends. He's played with everyone, from John Lennon to Dusty Springfield. Right now, Bob is providing the low end for the Crosby, Stills and Nash tour. Click here … more »
Mike Inez Rocks Massive Ampeg Rig for N. American TourFebruary 24, 2010
Alice in Chains has already begun their North American tour and Ampeg endorser/bassist extraordinaire Mike Inez has brought along one of the most massive Ampeg bass rigs that we have ever seen. This monster rig combines two SVT-810E's and four … more »
Tony Levin and Stick Men Rock NAMM with the Micro-VRFebruary 8, 2010
One of the highlights of the 2010 Winter NAMM show was undoubtedly the performances of Tony Levin with the Stick Men in Ampeg's demo room. Two separate performances, each unique in both vibe and flow, packed the house with fans … more »
Two Jons. Too Awesome.November 17, 2009
Ampeg endorser Jon Reshard recently sat in with drummer, Jon Wilkes to jam out a track from Reshard's latest self-titled album. What transpired is more than inspirational for anyone who has ever picked up a guitar or sat behind the … more »
Three Nights of Ampeg Artists on ConanNovember 3, 2009
Ampeg Artists play all over this great blue sphere. Some of them make their way onto popular television programs such as The Tonight Show with Conan O'Brien. Each time we see an Ampeg Artist doing their thing on national television, … more »
Conrad Korsch & the Micro-VR StackOctober 6, 2009
Conrad Korsch is a New York City based acoustic and electric bassist/multi-instrumentalist, composer, producer, and educator. As bassist with Rod Stewart, Carly Simon, Andrea Bocelli, Bette Midler, Clay Aiken, Deborah Gibson, the Broadway show Swing!, the annual Jerry Lewis/MDA Telethon's … more »
It's a Micro, Micro WorldOctober 5, 2009
The Ampeg Micro-VR stack (Micro-VR head & SVT-210AV cab) has been making some pretty big waves with professional bassists. In fact, we've been getting a lot of orders from top-named artists who want to get their hands on this little … more »


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Quick And Easy Healthy Mussel Omelette Recipe
Looking for a light dish for those balmy summer evenings? A mussel omelette is a quick and...276
Looking for a light dish for those balmy summer evenings? A mussel omelette is a quick and...276
Breakfast is not only the most important meal of the day, but it's arguably the most delicious....13
If you're stuck for an easy dinner recipe and desperate to try something new, these smoked trout patties...47
This egg frittata is perfect for breakfast, brunch, or let's face it – any time of the day!...38
Who can resist a perfectly poached egg, especially when it's served with smashed avocado, hollandaise sauce and...24
The initial thought of a crepe might bring about a mental image of sugar, chocolate, with a...369
Start your morning the right way with a healthy wild mushroom omelette which is sure to keep...14
A healthy frittata dinner makes for delicious leftovers for lunch or even dinner the next day, since...15
There is no denying that eggs make the perfect breakfast recipe; packed with protein, and so quick...209
Make this healthy breakfast or brunch meal which is filled with protein and will keep you full until...23
We've all experienced it before, right? A delicious poached egg made by a friend or from your...24
Callum Hann's frittata recipe from his fun new cookbook I'd Eat That make great summer picnic food,...2,083
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Everything's better with an egg on it! This creamy mushroom soup flavoured with thyme and chives is...590
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Start the day right with these cafe-style eggs wrapped in baby spinach, with a side of roasted...503
In this frenetic age in which we live, we seldom have time to settle down and enjoy...375


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Transfer Credit
McKendree University accepts all applicable college credits with a grade of "C" or
better from any regionally accredited institution of higher learning. A maximum of
90 credit hours in total may be accepted in transfer from a regionally accredited
4-year institution or from a combination of hours from a 4-year institution and a
junior or community college. All other admission requirements must be met.
Please visit the transfer information page under Academic Records for more information.


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Added by Brooke Willson
Distance: 10km round trip. Elevation gain: 800m. 360 views of Kananaskis. Scenery is beautiful the whole way. A perfect intro scramble for those wanting to get into more advanced scrambling. The perfect sunset or sunrise hike.
Rated as moderate to difficult due to scrambling sections. Estimated round trip is 4-5 hours depending on experience. From highway #1, the trailhead is located 13 kms South on highway 40. You will spot a small stream on your left with a small parking area for a few cars. The trail head is often marked with orange or red hiking tape, though its quite visible from the parking location.
The initial 1-2 kms are quite steep, as you make your way through the forest and soon into an exposed area, with gorgeous views. It doesn't take long to gain elevation! You will continue on until you reach a point where you can either go left up a steep challenging scramble bed (highly recommended for those who are comfortable climbing and wanting a gorgeous photo opt) those who would rather skip this section can veer to the right bypassing the initial scramble and can meet up with the crew approx. 200-300m later.
From here you continue along a ridge and will arrive at an open space where people have built a rock couch and fire pit, with a view of Mt. Baldy summit behind it. A perfect spot for a break and snack!
From here you will continue to work your way up 'zig zagging' to the top - there isn't one set trail. Be cautious with foot placement and hand holds (lose rock is common). Once you reach the top you can sit and enjoy the view and work your way back down or you can traverse a ridge to a shorter second summit if you are feeling ambitious, and if conditions allow you to do so safely.
Pack List
- Day pack
- Waterbottle
- Snacks to keep you energized
- Hiking poles
- Bear spray
- Camera
- Sunscreen/sunglasses/hat when weather permits
ReviewsLeave a Review
3 months ago
Good Hike But A Lot Of Shale
This was a good hike but there is a lot of shale and loose rocks.
over 1 year ago
Great View!
I would say it was the hardest hike so far. If you love scrambling. Yeah this is right place.
Added by Brooke Willson
24 years old, currently living in Central Alberta. Having obtained my Social Worker Degree, I am currently employed as a FT Youth Worker and Resiliency Coach at a K-8 school in my home town. Working school hours has its benefits, as I am able to spend every weekend as well as holidays and summer months out adventuring, exploring and traveling. Kayaking, hiking, biking, running, photography and camping are some of my favourite activities. Also living so close to the Rocky Mountains I am blessed with endless trails, scenery and opportunity to explore. With 14 countries under my belt I want to and will continue to explore our beautiful world on an International level.Follow
Skill Level:
Spring, Summer, Autumn
Are we missing something?Suggest an edit
More Adventures Nearby
Hike to Bonnell Creek Falls
Canada / Bonnell Creek Falls Trail
This trail isn't much to look at but it's all about the destination here as you will stumble upon scenic waterfalls nestled in the quiet Nanoose Bay area.


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Phytoestrogens and Memory Decline in Menopause
This study has been completed.
National Center for Complementary and Integrative Health (NCCIH)
Information provided by:
National Center for Complementary and Integrative Health (NCCIH)
ClinicalTrials.gov Identifier:
First received: January 9, 2003
Last updated: October 24, 2007
Last verified: October 2007
- Full Text View
- Tabular View
- No Study Results Posted
- Disclaimer
- How to Read a Study Record
No Study Results Posted on ClinicalTrials.gov for this Study
|Study Status:
|This study has been completed.
|Study Completion Date:
|August 2007
|Primary Completion Date:
|No date given


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Exterior Design Blogs
Home decors,get information about the house here, such as the design of the living room,small living.. room design,home decors,bedroom design,interior design
Owner: surya23
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Home furniture, Office furniture, Bedroom furniture, Furniture stores, Outdoor furniture, Indoor.. furniture, Home Interior Design, Office Interior Design, All about Interior Design and furniture
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Provide home design, house design, house decorating, modern home plans, interior design,.. contemporary room space design, such as living room,
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4. Home Designs
home design ideas | interior design | exterior design | home decor | home improvement
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6. Home Architecture Design, Interior Design Ideas, Exterior Design Ideas, Ashley Furniture, Patio Furniture
eblandar.com This blog displays interior design, home interior ideas, home interior design,home.. interior design ideas,interior design ideas,interior design photos,home interiors
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How to Build your Home and All Information about Building Home
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Interior Design Magazine offers the latest interior design trends, ideas, contemporary architecture.. and design news.
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The largest collection of interior design and decorating ideas online,including.. kitchens,bathrooms,Bathroom Interior,Bedroom Interior,Cafe interior,Countertops,Dinning Interior,.. Fireplace,Home Ceiling Design,Home Interior,etc.
Owner: ahmas
Listed in: Home-garden
Other Tags: kitchen, interior idea, living room, sunroom, bathroom interior


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2017-08-22 19:19:37
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Recipes - Mozzarella and Tomato Appetizer Tray (Caprese Italian recipe)
In a medium bowl, mix together parsley, basil, oregano, capers, garlic and olive oil.
On a serving platter, arrange mozzarella cheese slices and tomato slices in alternating layers. Top with roasted red peppers. Drizzle with the herb and olive oil mixture. Cover and chill in the refrigerator 30 minutes before sprinkling with crushed red pepper and serving.
1 tablespoon minced fresh parsley
1/2 teaspoon dried basil
1 pinch dried oregano
1 tablespoon capers, drained
2 cloves garlic, minced
6 tablespoons olive oil
1 pound mozzarella cheese, sliced
2 tomatoes, thinly sliced
1 (7 ounce) jar roasted red peppers, drained and julienned crushed red pepper to taste
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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Need a timer to set a time limit in the formsAsked by Francisco Gonçalves on November 17, 2015 at 01:14 PM
Dear all,
I've been using survey monkey for my experiments. However I need a timer to set a time limit for each trial be answered within my experimental blocks.
Is this possible to do in your platform? And can it be done in which version by which amount per year?
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University of Leicester, UK
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Sunset in Paris :)
|August 5th, 2015 at 7:16 am
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On the picture:: Sunset in Paris
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Free show, movie, July 5 in Fremont: Smorgaschord + "Music Man"
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- Get enough sun? Maybe its time for a cool matinee? On Saturday,
July 5, Center Theater in Fremont ( http://www.centertheater-info.com
) is having a special screening of the movie "The Music Man". This
event is sponsored by the Center Theater Preservation Group and
spearheaded by volunteer Andrew MacRae. Admission is free, doors
open at 3:30. The show kicks off at 4 pm with a set of a cappella
singing by Smorgaschord ( http://smorgaschord.com ). A prize
giveaway, in part sponsored by Round Table Pizza, starts at 4:30.
The Music Man starts at 4:45.
The purpose of this event is to keep the conditional use permit on
the theater valid. An event must be held once every 6 months to keep
this permit valid. There are costs associated with this event and
the theater is requesting donations of those in attendance (passive
requests... no bucket will be passed!). Additionally, concession
sales will help offset costs.
For a fun, cool afternoon, plan on attending the Music Man at the
Center Theater!
The Center Theater Preservation Group
map: http://maps.yahoo.com/py/maps.py?Pyt=&ed=UIsB
Center Theater
37411 Fremont Blvd
Fremont, CA 94536-3740
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Archive for
NEWS, prophecy, dreams, ZionsCRY, Bible, teaching, visions
cj.myfreeforum.org Forum Index
Who is Doing the Forgiving!
Who in the world are those involved in religion asking for forgiveness? From God! Therefore the assumption is, Gods not reconciled when they sin. In their mind they are separating themselves from God by their sin. Justification...Redemption...Propitiation...Remission...and Forbearance...all terms God wants people to acknowledge, to become familiar with, and to come to fully appreciate. Never make the mistake of thinking that these terms all mean the same thing. They do not. Each one has a distinct meaning all its own. Oftentimes, people will look at these terms and sort of lump them all together under a heading called forgiveness.
To many people are trying to Christianize the flesh. One should never drive the train from emotions, but there are denominations living in the caboose, driving the train from the emotion standpoint, not living it from the doctrinal standpoint. But think about this: Is forgiveness the same thing as being justified or having someone elses righteousness freely attributed to their account? They are not one and the same. The human races sins have all been forgiven, but forgiveness alone does not mean the person having forgiveness is as perfectly and totally righteous as the one who is doing the forgiving.
Acts 26:20 But shewed first unto them of Damascus, and at Jerusalem, and throughout all the coasts of Judaea, and then to the Gentiles, that they should repent and turn to God, and do works meet for repentance.
1 Corinthians 6:9 Know ye not that the unrighteous shall not inherit the kingdom of God? Be not deceived: neither fornicators, nor idolaters, nor adulterers, nor effeminate, nor abusers of themselves with mankind,
1Co 6:10 Nor thieves, nor covetous, nor drunkards, nor revilers, nor extortioners, shall inherit the kingdom of God.
1Co 6:11 And such were some of you: but ye are washed, but ye are sanctified, but ye are justified in the name of the Lord Jesus, and by the Spirit of our God.
Colossians 3:1 If ye then be risen with Christ, seek those things which are above, where Christ sitteth on the right hand of God.
Col 3:2 Set your affection on things above, not on things on the earth.
Col 3:3 For ye are dead, and your life is hid with Christ in God.
Col 3:4 When Christ, who is our life, shall appear, then shall ye also appear with him in glory.
Col 3:5 Mortify therefore your members which are upon the earth; fornication, uncleanness, inordinate affection, evil concupiscence, and covetousness, which is idolatry:
Col 3:6 For which things' sake the wrath of God cometh on the children of disobedience:
Col 3:7 In the which ye also walked some time, when ye lived in them.
Col 3:8 But now ye also put off all these; anger, wrath, malice, blasphemy, filthy communication out of your mouth.
Col 3:9 Lie not one to another, seeing that ye have put off the old man with his deeds;
Col 3:10 And have put on the new man, which is renewed in knowledge after the image of him that created him:
Col 3:11 Where there is neither Greek nor Jew, circumcision nor uncircumcision, Barbarian, Scythian, bond nor free: but Christ is all, and in all.
Col 3:12 Put on therefore, as the elect of God, holy and beloved, bowels of mercies, kindness, humbleness of mind, meekness, longsuffering;
Col 3:13 Forbearing one another, and forgiving one another, if any man have a quarrel against any: even as Christ forgave you, so also do ye.
Col 3:14 And above all these things put on charity, which is the bond of perfectness.
1 Corinthians 6:9-10, if Paul had ceased writing after going through this list of deplorable practices then we could safely assume that continuing to do these things after believing the good news message would result in a reversal of the offenders salvation or as some teach perhaps even a lost with a connect with God that must somehow be restored. But that isnt what Paul is saying here at all. Paul is telling these carnal saints at Corinth that the reason these detestable deeds had not and could not result in a lost of their salvation or their fellowship with God for that matter is because as carnal as these Corinthians happen to be they had a holy standing with God through their identity with his son. And the gift decree of righteousness belonging to the son had been totally and permanently credited to the account of these carnal believers to their account in heaven at the point of their belief.
Little wonder that right after listing all these deplorable sins the apostle Paul follows up immediately in verse 12 with these words All things are lawful unto me, but all things are not expedient: all things are lawful for me, but I will not be brought under the power of any. So while none of the things on Pauls list either 1 Corinthians chapter 6 or we might even say Galatians chapter 5 where Paul presents a similar list...none of these things can send a believer to the second death. They are definitely things all believers should avoid.
cj.myfreeforum.org Forum Index


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Centers for Disease Control and Prevention
Phone: 770-488-7100 (24 hours)
Domestic Preparedness Chemical/Biological HelpLine
Phone: 800-368-6498
Provides technical assistance during business hours to eligible State and local emergency responders and their organizations.
Chem/Bio Hotline 800-424-8802 (24 hours)
Radiological Hotline 202-586-8100 (24 hours)
Report a Chemical, Biological, or Radiological emergency incident.
FEMA, Office of National Preparedness: Preparedness News and Information (May 30, 2003)
National Response Center Hotline
Phone: 800-424-8802 (24 hours)
Receives reports of oil, chemical, biological, and radiological releases and actual or potential domestic terrorism; provides technical assistance to emergency responders; and connects callers with appropriate Federal resources.
Nuclear Regulatory Commission Operations Center
Phone: 301-816-5100 (collect calls accepted)
Accepts reports of accidents involving radiological materials.


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
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Blue Air wishes to announce a new direct flight between Glasgow and Bucharest.
Tickets are already available at prices starting from* £26.55 so book your trips in advance!
Flights will be operated from December 17, 2015, each Thursday and Sunday, throughout the winter schedule.
Book your tickets now on www.blueairweb.com, through the Call Centers or our partner agencies.
We like our customers to be informed. Therefore, regardless of the doubt you have, you can contact our Call Center consultants, numbers are available here.
You can always contact us free of charge by submitting a question.


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An introduction to leading profound social, environmental and personal transformation.
Kelvy Bird has been working in the field of human development within organizational contexts since 1995, with a focus on collaboration, innovation, leadership, and collective intelligence. Her joy is in harnessing group creativity for projects that cultivate learning and connectivity. She has supported the Presencing Institute to develop the Global Forum, Global Classrooms, and online community spaces, and now serves as Creative Developer for ongoing PI initiatives.
Kelvy also works extensively as a founder and graphic facilitator with dpict llc, a visualization firm specializing in scribing to advance social understanding. She supports groups with live translation of content and dynamics into visual, tangible formats to aid with decision-making and reflection. Long-standing partners have included: The Value Web, MIT Exec Education, Harvard Grad School of Education, The Ashland Institute, Dialogos, and MG Taylor, among others that include Fortune 500 and local community organizations alike.
An artist at core, Kelvy addresses threads of personal inquiry that include the blossoming of human presence, the state of the heart, and the nature of cohesion. Through entirely abstract, subjective mark-making, she wraps her spirit around the complexities of our times and finds tap roots of sense.
Kelvy received her BFA in painting and BA in Art History from Cornell University. Her current residence is Massachusetts, USA. Email: contact(at)kelvybird.com
More info here.
An introduction to leading profound social, environmental and personal transformation.
Learn how to lead change in social systems by exploring two root questions of creativity: Who is my Self? What is my Work?


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|By Theavatardemotivator
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Mornings pass the most rapidly. Bolin is asleep when Mako seeks breakfast, rooting in dumpsters, nicking from the bazaar, stealing abandoned items. He never thinks of himself as a thief, and he knows he won't be caught: The spirits are looking out for them. Still, he prefers to take what has been left, not what will be used.
By the time his little brother is awake, fruit or leftovers or—on rare occasion—heated meat greets him on some days. On others, his stomach rumbles until lunch or even dinner, and on a few he returns to slumber without a bite at all.
Even though Bolin is six, he rarely complains. A scary stillness has settled over him. No, not stillness. Optimism. An optimism that blinds him to the bad and opens his eyes to the good. Warm, hug, delicious, play, brother. Words that pepper his dialogue like a virus, spreading through his entire body, burying his hurts, fears, and aches in another terror.
Mako isn't sure if his brother truly is this optimistic or if he is merely afraid of provoking the firebender's rage. He hopes it is the former.
Afternoons are sluggish. Bolin practices his bending, his every accomplishment buoyed by words of encouragement and shouts of success. A pebble. Two. Three. Rocks that zoom around. Careful constructions of soil.
Once, he finds a glimmering black screw from a Satomobile, the thread thin and winding but broad enough to be traced with a fingernail. In the darkness, Mako feels Bolin remove it from his pocket and marvel at the construction, often trying to bend it and failing, sometimes pressing it into his brother's hand and begging him to admire its simplicity, its basic design, and its power.
The nights are the worst. They shelter under the statue of Fire Lord Zuko, the fire constantly burning in his outstretched hand a needed beacon of light and hope. Mako learns to ignore the stench of lizard crow droppings and their own dirty clothing, pretends not to hear the stinging remarks of those older and more fortunate than he—even if he only understands the message by the tone and the tautness of the jawline—and refuses to believe that he once had a life outside of this one.
It becomes less of a lie and more of a legend. Maybe if he says it enough times, it will come true.
But it doesn't.
It never will.
Protected from the chill and the wet by his brother's form around him, the earthbender misses being rocked by Mommy, and he wishes he could ride in a Satomobile. "It feels almost the same," he explains, his face tucked into Mako's armpit to hide from the monsters of the night, his words lost in the folds of the scarf wound around them both. "Mako, can you rock me?"
His arms hurt, but he gently sways, stopping after he hears Bolin's snoring, the sweetest sound he has ever heard. A fire dances in his hand, the shadows blurring the filth of their new home into smooth.
Time passes, the day melting into each other. The clouds weep bitters tears of a grief alien to his heart, a grief he will not comprehend for years down the path, a grief that leaves scars in his spirit and rips him in half. Worse than the rainfall is the old, the never-ending cold. At least his firebending offers them some consolation. The others, children as lost as the brothers, slip away in the darkness, their eyes—the first to defrost—pecked out by morn, the lizard crows starting on the soft flesh of the cheeks and throat once the thin barrier of cold is gone.
The scarf shields Bolin from the worst, but Mako forces himself to look at the rivulets blood trickling from the mouths, listen to the moans of those sickened with fever, smell the decay on their bodies before they perish, a smell not unlike that lingering on the brothers' rotting, loose clothes. All of the dead are sad, but the saddest are the littlest ones, their skins pale as the spirits departed from this world, their gazes frozen to the sky, their swollen lips slightly parted as if in prayer or calling out to loved ones, to Mommies and Daddies waiting for their precious flowers to join them. Fingers curl around stalks of grass peeking through the snow and cement or claw at throats tinged blue with bruises brought of desperation in dehydration.
On the street, he muses, one bends, or one dies.
Water is easy to come by. If it does not rain, dew covers the grass, and if that fails, the snow comes with increasing frequency, and heat funnelled from flame brings cool liquid to the dryness in their mouths. He worries of summer, but he is certain he will find a way.
He will find a way, for his brother.
Bolin helps. With his angelic face, innocent eyes, and cattish smile, he attracts offerings of coin and cooking, cautiously set down by kindly women. It aids him to wear the scarf, absurdly long for his stature, short for his age of merely six, and to take on earthbending stances or make overtly cute observations befitting a toddler of two. Anything works, and the rags hide the ribs, ruining the picture painstakingly perfected to please the passers-by.
The lizard crow with the missing foot mocks Mako, morning or midnight. Obviously, he is the leader of the murder, his caws commands for his kin to heed, the V-like pink across his otherwise black-feathered chest an easy marker for identification. He takes Bolin's charity with gusto—Mako is sure the bird's nest is lined with glittering gold—and swipes meals about to be collected by the firebender.
After a time Mako reverts to stealing from others. It's easier and quicker for him, and it finds them food that fills.
He does not believe that he will be caught until he is.
See more
For the collective works of the author, go here.


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Polymorphonuclear leukocyte-dominated airway inflammation is a major component of cystic fibrosis (CF) lung disease and may be associated with CF transmembrane conductance regulator (CFTR) dysfunction as well as infection. Mutant ΔF508 CFTR is mistrafficked, accumulates in the endoplasmic reticulum (ER), and may cause "cell stress" and activation of nuclear factor (NF)-κB. G551D mutants also lack Cl− channel function, but CFTR is trafficked normally. We compared the effects of CFTR mutations on the endogenous activation of an NF-κB reporter construct. In transfected Chinese hamster ovary cells, the mistrafficked ΔF508 allele caused a sevenfold activation of NF-κB compared with wild-type CFTR or the G551D mutant (P < 0.001). NF-κB was also activated in 9/HTEo−/pCep-R cells and in 16HBE/pcftrantisense cell lines, which lack CFTR Cl− channel function but do not accumulate mutant protein in the ER. This endogenous activation of NF-κB was associated with elevated interleukin-8 expression. Impaired CFTR Cl− channel activity as well as cell stress due to accumulation of mistrafficked CFTR in the ER contributes to the endogenous activation of NF-κB in cells with the CFTR mutation.
- nuclear factor-κB
- cystic fibrosis transmembrane conductance regulator
- chloride channel
- inflammatory response
- intracellular calcium
- mitogen-activated protein kinase
cystic fibrosis (CF) transmembrane conductance regulator (CFTR) mutations have many effects on the physiology of respiratory epithelial cells in addition to the expected effects on Cl−transport. One of the major clinical manifestations of CFTR mutations is excessive airway inflammation (14-17), implying that CFTR affects the immune function of airway epithelial cells, particularly the expression of nuclear factor (NF)-κB-dependent proinflammatory chemokines and cytokines. Several lines of experimental evidence suggest that CFTR dysfunction influences the exogenous activation of epithelial immune function (8, 30). Bacteria persist in the respiratory tract of CF patients due to decreased antimicrobial activity in CF airway surface fluid and impair bacterial clearance due to mucus plugging (25). Cells with CFTR mutations have increased numbers of asialoglycolipid receptors for common bacterial pathogens (24). Ligation of these receptors activates NF-κB-dependent epithelial interleukin (IL)-8 production to recruit polymorphonuclear leukocytes (PMNs) from the circulation into the airways (8). Epithelial expression of IL-8 after bacterial stimulation is increased in cells with CFTR dysfunction, a finding demonstrated in both matched CF and corrected cell lines (5, 9, 27, 30) and in cftr-deficient [cftr(−/−)] mice compared with normal control mice (29). How CFTR dysfunction might affect the endogenous expression of NF-κB-dependent genes is less well documented.
NF-κB is a transcription factor required for the expression of many genes important in inflammation. It is complexed in the cell cytoplasm with IκB-α and -β, which are selectively phosphorylated, ubiquitinated, and degraded in the proteasome in response to stimuli (2). There is both signal-dependent and signal-independent IκB-α phosphorylation (20), a distinction that may be relevant to the endogenous activity of NF-κB as opposed to the activation in response to a superficial stimulus such as bacterial attachment to surface receptors. Ligation of superficial asialo-GM1 receptors on epithelial surfaces by bacterial adhesins stimulates Ca2+-dependent kinase activity through mitogen-activated protein kinase phosphorylation and NF-κB activation (23a). NF-κB translocation may be regulated by other intracellular factors that lead to a different pattern of IκB-α phosphorylation than that elicited by extracellular stimuli (4). It has been proposed that the abnormal trafficking of ΔF508 CFTR and its accumulation in the endoplasmic reticulum (ER) provides sufficient intracellular stress to cause NF-κB activation (2).
The most common CFTR mutation responsible for CF in ∼70% of patients is the deletion of a phenylalanine residue (ΔF508) in the nucleotide binding domain. The ΔF508 mutant CFTR not only fails to transport Cl− in response to cAMP but accumulates in the ER due to abnormal maturation and folding (1, 7, 32). From experimental data generated with adenovirus mutants targeted for retention in the ER, Pahl and colleagues (18, 19) proposed that homozygous ΔF508 mutations in CFTR would similarly cause Ca2+ release, activation of NF-κB, and resultant transcription of proinflammatory cytokines and chemokines. Comparison of the endogenous activation of NF-κB in IB3 cells (W1282X/ΔF508), a trafficking mutant similar to the homozygous ΔF508 mutation, with that in corrected C-38 cells, which express a functional but truncated form of CFTR, was consistent with this hypothesis. The transcriptionally active p65 component of NF-κB was found in the nuclei of unstimulated CF cells but not of the corrected cells by immunofluorescence labeling and was confirmed by gel shift assays (8). Manipulations to increase CFTR trafficking to the apical surface of the cell, such as growth at low temperature or the addition of glycerol, decreased the amount of p65 found in the IB3 cell nuclei. Although these observations were consistent with the hypothesis that abnormal CFTR proteins cause endogenous activation of NF-κB, they did not differentiate between two possible mechanisms: that "cell stress" associated with CFTR mistrafficking leads to changes in intracellular Ca2+ concentration ([Ca2+]i) and stimulation of NF-κB translocation or that CFTR Cl− channel dysfunction by itself is associated with endogenous activation of NF-κB.
Other types of CFTR mutations cause "CF-like" physiology, i.e., lack of Cl− secretion in response to cAMP, without causing trafficking abnormalities or resulting in the accumulation of mutant proteins in the ER (7). Analysis of the activation of NF-κB in such cell lines would provide the opportunity to determine whether Cl− channel dysfunction alone is associated with the regulation of NF-κB. The G551D CFTR mutant, in which a conserved glycine in the ATP binding cassette is mutated, lacks Cl−channel function, but the protein is properly folded and trafficked (12). Patients with the G551D mutation have a clinical disease that is indistinguishable from that caused by the more common ΔF508 mutation (10). The 9/HTEo− human tracheal epithelial cell line transfected with a plasmid directing constitutive expression of the CFTR regulatory (R) domain also fails to transport Cl− in response to cAMP while expressing low levels of wild-type CFTR message detected by RT-PCR (21). These 9/HTEo−/pCep-R cells are readily activated byPseudomonas aeruginosa and express significantly more IL-8 than control cells containing the empty vector (5). The impact of impaired CFTR Cl− channel activity on the normal interaction of IκBs and NF-κB is not known. Recently constructed 16HBE cell lines expressing plasmid-encoded cftr in the sense or antisense orientation also provide the opportunity to test the impact of CFTR Cl− channel function on the activation of NF-κB in airway cells without the confounding issues of mistrafficked CFTR protein (23).
In the experiments detailed in this report, several matched cell lines with CFTR dysfunction due to lack of Cl− channel function, CFTR misfolding, mistrafficking, or both were tested for endogenous activation of NF-κB and basal IL-8 expression. Although CFTR mistrafficking typical of the common ΔF508 mutation and associated cell stress may be responsible for the increased expression of proinflammatory cytokines and chemokines associated with CF cells, we sought to establish whether CFTR Cl− channel function as well as the effects of CFTR mistrafficking contributes to endogenous activation of NF-κB and the excessive inflammation that characterizes CF airway pathology.
Epithelial Cell Lines
9/HTEo− cells, a human tracheal epithelial cell line (11), were grown in DMEM supplemented with 10% FCS and were stably transfected with either pCep, a vector control, or pCep-R, providing constitutive expression of the R-domain of CFTR resulting in typical CF-like physiology, i.e., failure of Cl− secretion in response to cAMP (21) and altered superficial sialylation (5). IB3 cells (ΔF508/W1282X), a human bronchial epithelial cell line, and C-38 cells, "corrected" cells with normal physiology that express an episomal truncated form of CFTR (33), were obtained from P. Zeitlin (Johns Hopkins University, Baltimore, MD) and grown in LHC-8 medium (Biofluids, Rockville, MD) plus 10% FCS. The IB3 cells used in these studies contain the empty vector. 16HBE cells transfected with plasmids expressing the first 131 nucleotides of cftr in either the sense or antisense orientation were grown in MEM plus 10% FCS plus l-glutamine and have been previously described (23). Cells expressing the antisense plasmid failed to respond to cAMP agonists with Cl− secretion. Stably transfected Chinese hamster ovary (CHO)-K1 cells that express ΔF508 CFTR or G551D CFTR were obtained from J. Riordan (Mayo Clinic Scottsdale, Scottsdale, AZ) and grown in αMEM plus 200 μM methotrexate plus 8% FCS. CHO-K1 cells were obtained from the American Type Culture Collection (ATCC) and lipofected with pCep plasmid constructs expressing either wild-type CFTR, G551D CFTR, ΔF508 CFTR, or the vector alone, provided by M. Drumm (Case Western Reserve University, Cleveland OH). CHO cells were grown in MEM plus 10% FCS. CFTR expression in the cells was verified by immunodetection with COOH terminus-specific mouse monoclonal anti-CFTR antibody (Genzyme, Minneapolis, MN).
IL-8 Assays
RT-PCR analysis.
Total RNA was isolated from confluent monolayers of IB3 and C-38 cells grown in 10-cm plates with RNeasy Mini Kit, and RT was performed with Omniscript RT (both from QIAGEN, Valencia, CA). PCR primers for human IL-8 were 5′-TACTCCAAACCTTTCCAACCC-3′ and 5′-AACTTCTCCACAACCCTCTG-3′. The cells were stimulated by the addition of 0.5 ml of P. aeruginosa PAO1 (5 × 109 colony-stimulating units/ml) scraped from an agar plate and resuspended in cell culture medium.
IL-8 in epithelial cell culture supernatants was measured with ELISA (R&D Systems, Minneapolis, MN). The cells were weaned from serum for 18 h, and the supernatants were harvested 18 h later for IL-8 ELISA. Duplicate wells were treated with trypan blue to assess epithelial viability during the assay, which was >75%. Each IL-8 data point was determined in quintuplicate, and means ± SD were calculated. Significance was evaluated with a one-way ANOVA with Dunnett's posttest (GraphPad Instat version 3.00, GraphPad Software, San Diego, CA) to test the null hypothesis that there was no difference in the amount of IL-8 produced by the epithelial cells with different CFTR function.
Activation of NF-κB Detected by Luciferase Reporter Constructs
Cells were grown in six-well plates to 80–90% confluence, washed once with PBS, and replated with serum- and antibiotic-free medium. The cells were transiently transfected with LipofectAMINE 2000 reagent (Life Technologies, Gaithersburg, MD), 1 μg of each plasmid DNA, pNF-κB-Luc (Stratagene, La Jolla, CA) and a constitutively active pRL-TK (Promega, Madison, WI), to control for transfection efficiency and incubated at 37°C in 5% CO2 for 18 h. Lipofection of the CHO cells was done with an additional 3 μg of pCep-CFTR vector DNA. The cells were washed once, lysed, and harvested with passive lysis buffer (Promega). Luciferase assays were performed with the reagents and protocol for the dual-luciferase reporter assay system (Promega) and analyzed with a luminometer. After standardization for transfection efficiency, data were plotted as the means of quadruplicate samples and are representative of at least three independent experiments. Statistical analysis was performed with an unpaired t-test for the experiments involving epithelial cells and a one-way ANOVA to analyze the results obtained with the CHO cells to accommodate a larger sample size.
[Ca2+]i in IB3 and C-38 Cells
Relative levels of [Ca2+]i were estimated in fluo 3-loaded IB3 and C-38 cells by fluorescence imaging. Monolayers of C-38 or IB3 cells were grown for 2 days on Lab-TekII chambered slides (Nalge Nunc International, Naperville, IL) in LHC-8 medium (Biofluids) supplemented with 10% FCS. The monolayers were washed and dye loaded with fluo 3-AM (Bio-Rad, Hercules, CA) for 0.5 h at room temperature in the dark in modified Ringer solution (145 mM NaCl, 5 mM KCl, 1 mM Na2HPO4, 2 mM CaCl2, 10 mM glucose, 20 mM HEPES, and 1 mM MgSO4, pH 7.4). The cells were rinsed in Ringer solution and incubated for 30 min to allow continued cleavage of the acetoxymethyl ester. The monolayers were imaged on a Zeiss Axiovert microscope at ×40 magnification with Inovision software. Images were taken at 10-s intervals before and after the addition of stimuli, and each image is the average of eight scans. Final measurements were taken after the addition of the ionophore 4-bromo-A-23187 (Bio-Rad) to allow for correction of differences in dye loading. Images were analyzed with Scion image software (Scion, Frederick, MD). Significance was determined with an unpaired t-test to compare the mean relative fluorescence of the two populations of epithelial cells.
Airway epithelial cells were grown to near confluence on Lab-TekII eight-chambered glass slides (Nalge Nunc International). They were washed with PBS, fixed with 4% paraformaldehyde in PBS for 20 min at room temperature, washed three times with PBS, and blocked in 10% goat serum in Ca2+/Mg2+ PBS for 1 h at room temperature. After blocking, the cells were washed three times in PBS and incubated with a 1:100 dilution of rabbit polyclonal antibody p65 (Santa Cruz Biotechnology, Santa Cruz, CA) for 1 h at room temperature, washed, and then incubated with goat anti-rabbit IgG conjugated to tetramethylrhodamine B isothiocyanate (TRITC; 1:50; Zymed, South San Francisco, CA) in PBS plus 0.1% BSA plus an equal volume of bis-benzimide (50 μg/ml) for 1 h at room temperature. The cells were imaged on a Zeiss Axiovert S100 microscope with a Hamamatsu Orca II digital camera and Inovision software. 560D15 and 400DF15 band-pass filters were used for TRITC and bis-benzimide imaging, respectively. Emission data were collected with a triple-pass filter at wavelengths of 460 nm for bis-benzimide and 602 nm for TRITC.
Activation of NF-κB in Epithelial Cells With CFTR Dysfunction
To determine whether lack of CFTR-associated Cl−channel function affects the basal activation of NF-κB in airway epithelial cells, two cell lines with defective CFTR function but without mutations associated with mistrafficking were studied. 9/HTEo−/pCep cells and 9/HTEo−/pCep-R cells, which have constitutive overexpression of the CFTR R-domain and fail to transport Cl− in response to cAMP, were transfected with an NF-κB-luciferase reporter construct. The 9/HTEo−/pCep-R cells had 5.5-fold greater activation of NF-κB than 9/HTEo−/pCep cells expressing the vector control (Fig. 1). Similarly, 16HBE cells expressing cftr antisense, previously shown to lack CFTR-dependent Cl− channel activity, had fivefold greater activation of the NF-κB reporter than the cells expressingcftr in the sense orientation (P < 0.001 for both).
Nuclear p65 (Rel A) in Cells With CFTR Dysfunction Detected by Immunofluorescence
The presence of the p65 (Rel A) component of NF-κB in the cell nucleus is generally indicative of NF-κB activation and gene transcription. To verify that activation of NF-κB as detected by the reporter constructs was indicative of nuclear localization of the p65 component, the cell lines were permeabilized, treated with antibody to p65 that was detected with a goat anti-human IgG conjugated to TRITC with bis-benzimide to stain the nuclei. The control cells with normal CFTR function, either 9/HTEo−/pCep or 16HBE/cftr sense, had predominantly cytosolic p65 fluorescence (Fig. 2). In contrast, within the fields of 9/HTEo−/Cep-R and 16HBE/cftr antisense cells, there were significantly more cells with nuclear p65 TRITC fluorescence colocalizing with the blue bis-benzimide nuclear stain.
Activation of NF-κB in CHO Cells Expressing Wild-Type and Mutant CFTR
CHO cells that were transiently transfected with the pCep empty vector or pCep vector expressing wild-type CFTR had low levels of basal NF-κB activation as did the cells transfected with the plasmid expressing the mutant G551D CFTR (Fig.3 A). This was in contrast to the threefold increase in luciferase activity associated with CHO cells expressing mutant ΔF508 CFTR (P < 0.001 for ΔF508 compared with the pCep vector control). Experiments done with stably transfected CHO cells yielded similar results (Fig. 3 C); expression of ΔF508 CFTR was associated with a sevenfold increase in reporter activation compared with that in the CHO cells expressing the G551D mutation (P < 0.001) or CHO cells transfected with the reporter construct alone (P < 0.001). The relative expression of CFTR in the CHO cells was roughly equivalent (Fig. 3, B and D). In the CHO cells, expression of the ΔF508 CFTR mutation was sufficient to activate NF-κB, whereas the presence of comparable amounts of the mutant G551D CFTR was not.
IL-8 Expression Is Increased in Cell Lines With CFTR Dysfunction
The nuclear localization of NF-κB is associated with the transcription of several genes such as IL-8, a major PMN chemokine, that are important in inflammation. Increased expression of IL-8 has been frequently reported to be characteristic of CF cells after bacterial stimulation as well as under basal conditions (8). To determine whether the activation of NF-κB seen in the epithelial cell lines with dysfunctional CFTR is associated with an IL-8 response, IL-8 expression was assessed with RT-PCR (Fig.4). (These unstimulated cells did not produce sufficient amounts of IL-8 in serum-free medium to detect with a standard ELISA.) There was increased IL-8 mRNA expression detected in the IB3 cells compared with the endogenous levels in the C-38 cells, and both cell lines increased IL-8 mRNA expression in response to bacterial stimulation, included as a positive control.
IL-8 production could be reliably measured by ELISA in 9/HTEo− and 16HBE cell culture supernatants accumulated over 18 h after being weaned from serum. The 9/HTEo−/pCep-R cells produced more IL-8 than those expressing the vector control (P < 0.001), and lack of CFTR expression in the 16HBE cells expressing cftr antisense was also associated with increased IL-8 expression (P< 0.001; Fig. 5). Thus cell lines with defective CFTR Cl− channel activity, regardless of the nature of the CFTR defect, had increased IL-8 production compared with the corresponding cell line with normal CFTR activity.
[Ca2+]i in Cells With ER Overload
Epithelial cells with mistrafficked CFTR and "ER overload" have been postulated to have increased release of Ca2+ from ER stores as a consequence, a response that could activate Ca2+-dependent kinase activity. Relative [Ca2+]i was estimated in subconfluent IB3 and C-38 cells loaded with the fluorescent indicator fluo 3-AM (Fig.6). When the populations of the different cell types were compared, there was a consistent trend of increased [Ca2+]i associated with the IB3 cells, with relative basal fluorescence ranging from 0.6 to 0.85 compared with that in the population of C-38 cells, which had basal [Ca2+]i in the range of 0.5 to 0.75. Comparing the relative fluorescence of 10 individual cells at a single time point (30 s), the IB3 cells had a relative mean fluorescence of 0.758 ± 0.012 (SD) compared with 0.628 ± 0.08 for the C-38 cells (P < 0.001). Both cell types responded to the addition of P. aeruginosa with increased [Ca2+]i.
Airway inflammation is a major factor in the pathogenesis of CF pulmonary disease. Clinical studies have clearly established a direct correlation between infection, IL-8 secretion, and the accumulation of PMNs and their toxic products in the CF lung (14-17). Bacterial stimulation of cells with CFTR mutations activates significantly greater cytokine expression than matched cells with normal CFTR function (5, 8, 29, 30). Several reports (8, 28) suggest that cells with CFTR dysfunction have endogenously upregulated expression of proinflammatory cytokines and chemokines. Young infants with CF, even without detectable evidence of prior infection, have PMNs and increased amounts of IL-8 in their airways (14), and proinflammatory cytokine expression is increased in cells cultured directly from uninfected CF tissues (28). These findings are consistent with the in vitro studies demonstrating endogenous activation of NF-κB in CF but not in corrected cell lines. As indicated by the data in this report, lack of CFTR Cl− channel function and the physiological consequences of mistrafficked mutant CFTR both appear to contribute to the endogenous activation of NF-κB in CF airway cells, which is manifested by the increased expression of IL-8 in these cells, even in the absence of bacterial stimulation.
There is evidence of endogenous activation of NF-κB in cells with CFTR dysfunction due to several different mechanisms. In previous studies, IB3 cells, which express the W1282X/ΔF508 mutation associated with mistrafficked CFTR that accumulates in the ER, were found to have significant amounts of the p65 component of NF-κB in nuclei under basal conditions in which there was no nuclear NF-κB in the corrected C-38 cells (8). Because the translocation of NF-κB can be associated with many types of stimuli, additional consequences of accumulated mutant CFTR in the ER were also demonstrated in the mutant cell line. The IB3 cells were found to have higher basal [Ca2+]i, a predicted consequence of "ER stress" (2, 18). The presence of nuclear NF-κB in the IB3 cells could be correlated with increased IL-8 expression as estimated by RT-PCR compared with that in the control C-38 cell line. The concentration of IL-8 in the tissue culture supernatant was minimal in these cells after an 18-h incubation in serum-free medium; however, the relative amount of IL-8 message was consistent with a previous report by DiMango et al. (8). Not all investigators have found increased endogenous levels of IL-8 associated with CFTR mutations (30). Cell culture conditions, effects of FCS, and handling of the monolayers all can affect the translocation of NF-κB and endogenous expression of IL-8. Thus it seems relevant to demonstrate that the presence of nuclear p65 appears to be associated with the expected biological response, namely IL-8 expression.
The activation of other NF-κB-dependent pathways is not necessarily increased in IB3 compared with C-38 cells. Stimulation of NF-κB translocation is associated with an antiapoptotic effect in many cell lines including respiratory epithelial cells. Rates of apoptosis can be increased by proteasome inhibitors that inhibit IκB degradation and block NF-κB translocation (31). In a study published recently (23), the rates of IB3 and C-38 cell apoptosis were equivalent both under control conditions and after bacterial stimulation despite the observed differences in endogenous nuclear NF-κB. Thus the association between CFTR dysfunction, activation of NF-κB, and transcription of NF-κB-dependent genes is somewhat selective and is not a global response.
The consequences of CFTR mistrafficking and the activation of NF-κB were further demonstrated in CHO cells that do not normally express CFTR. The presence of the mistrafficked mutant ΔF508 CFTR was a significant stimulus for NF-κB activation in CHO cells. The G551D CFTR, which does not function appropriately as a Cl−channel but is trafficked normally to the apical surface of the respiratory epithelial cell (10), did not stimulate NF-κB. Expression of normal CFTR from the same plasmid construct did not cause NF-κB activation in CHO cells nor did transfection with the empty vector or the luciferase reporter construct. Although both the G551D and ΔF508 CFTR mutations are associated with clinical disease and lack of Cl− secretion in response to cAMP (10), the major difference between these mutants is the mistrafficking and accumulation of ΔF508 CFTR within the ER. Thus it appears that the consequences of mistrafficking of ΔF508 are sufficient to activate NF-κB in CHO cells.
In epithelial cells, unlike CHO cells, there appears to be a requirement for normal CFTR function in regulating NF-κB-dependent gene transcription. The homozygous ΔF508 or the compound ΔF508/W1282X mutation could activate NF-κB by two independent mechanisms: cell stress associated with mistrafficking or effects directly due to lack of CFTR Cl− channel activity. Cell lines that lack CFTR Cl− function due to either the overproduction of the CFTR R-domain or the expression of CFTR antisense had activated NF-κB and significant amounts of endogenous IL-8 production compared with the corresponding control cell lines. The amount of IL-8 endogenously expressed by these cell lines was greater than that of the IB3 or C38 cells and could be quantified by a standard ELISA assay. The presence of the p65 component of NF-κB in nuclei of undisturbed cells grown on coverslips as well as the relative activation of an NF-κB reporter construct compared with appropriate controls indicates that their activated state is unlikely to be an artifact of either cell transformation or manipulations occurring during cell culture. Because neither the 9/HTEo−/pCep-R or the 16HBE/cftr antisense cells have excessive CFTR accumulation in the ER, the lack of CFTR Cl− channel function in these epithelial cells appears to be responsible for the observed increase in NF-κB activation and IL-8 expression.
Exactly how CFTR dysfunction contributes to the activation and nuclear localization of NF-κB is unclear. There are multiple mechanisms for NF-κB activation that can be divided into signal-dependent and signal-independent pathways (20). In airway epithelial cells, bacterial attachment to asialo-GM1 activates a Ca2+-dependent signaling pathway that results in NF-κB activation and IL-8 transcription through a cascade that includes phosphorylation of both the p38 and extracellular signal-regulated kinase families of mitogen-activated protein kinases (23a). A signal-independent pathway, perhaps involving IκB or NF-κB regulation directly, may be affected by mistrafficked CFTR. IκB-β has been shown to be increased in IB3 cells under basal conditions and is hypophosphorylated, enabling nuclear NF-κB with bound IκB-β complexes to direct transcription (30). Hypophosphorylated IκB-β may be involved in either signal-dependent or the persistent activation of NF-κB (26). Diminished levels of cytosolic IκB-α was associated with CF (ΔF508/ΔF508) bronchial gland cells in primary culture and correlated with the presence of nuclear NF-κB Rel A (27). It is possible that CFTR function, perhaps by affecting pH in specific intracellular compartments (3), affects relevant kinase or phosphatase activity. Abnormalities in signal transducer and activator of transduction-1 phosphorylation in the same 9/HTEo−/pCep-R cells have been described recently (13), indicating that CFTR may have a role in multiple signaling pathways. These observations reflect the complexities of CFTR involvement in the normal physiology of the epithelial cell. Strategies such as gene therapy to correct CFTR Cl− channel function alone, which do not ameliorate the effects of mutant CFTR mistrafficking in the majority of airway epithelial cells, may not be sufficient to control the inflammatory component of CF lung disease.
These consequences of CFTR dysfunction in activating translocation of NF-κB and stimulating the expression of proinflammatory cytokines are important in not only understanding the many roles of CFTR in normal cell physiology but are also clinically relevant. The majority of CF patients with the most common ΔF508 CFTR alleles have multiple reasons for increased expression of inflammatory mediators in their airways. The effects of CFTR mistrafficking and ER stress, lack of CFTR Cl− channel function, and exogenous stimulation due to airway infection all act to increase epithelial NF-κB translocation and the expression of proinflammatory genes. This epithelial immune activation does provide multiple targets for therapy. It may be possible to devise a therapy to modulate the endogenous upregulation of NF-κB-dependent gene transcription without compromising the epithelial barrier function or the host inflammatory response to exogenous airway pathogens.
Expert technical assistance was provided by Robert Adamo.
This work was supported by National Heart, Lung, and Blood Institute Grants HL-56194 (to A. Prince) and P50-HL-60293 (to P. Davis, Case Western Reserve University, Cleveland, OH). Imaging studies were performed in the Optical Microscopy Facility of the Herbert Irving Cancer Center at Columbia University (New York, NY), supported by National Center for Research Resources Grant 1-S10-RR-10506.
Original submission in response to a special call for papers on "CFTR Trafficking and Signaling in Respiratory Epithelium."
Address for reprint requests and other correspondence: A. S. Prince, Black Bldg. 416, Columbia Univ., 650 West 168th St., New York, NY 10032 (E-mail: asp7{at}columbia.edu).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
- Copyright © 2001 the American Physiological Society


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I took a bus the night before from Kangar to Terminal Bersepadu Selatan (TBS). I arrived in TBS around 4.30am, reloaded my Touch n Go card and took the KLIA transit to KLIA2. The ERL fare from Bandar Tasik Selatan (BTS) to KLIA2 was around RM28. Since I wasn't in much of a hurry, I hopped off at Putrajaya, went out, punched my TnG card and went back in. I saved more than 50% of the overall fare doing so because the fare from BTS to Putrajaya was RM6.20, and Putrajaya to KLIA2 cost RM5.30. Try this trick whenever you are planning to save on money instead of time. I arrived in KLIA2, used the free shower stall in the departure hall to freshen up, met my brother and boarded. I was ready for the six hours flight to Seoul. ^_^
|Getting closer to Seoul..
We arrived around 4pm in Incheon International Airport. We bought the T-Money card (Korea's equivalent of a Touch n Go, just more efficient and widely used) in the 7E and boarded the subway to Gongdeok station. We arrived in our hostel near Itaewon, YaKorea Hostel Itaewon which I already reserved weeks earlier only to find out that it was closed for renovation. What a fate. But I guess it happened for a reason and thankfully it was not my mistake, so the manager found us a new hostel at the same price. If it wasn't for the incident, I would not be able to experience riding in a taxi for free (the fare was covered by the hostel), and our new room in the new hostel, Kimchee Seoul Station Guesthouse was better than the one I booked before. So yeah, I'm not complaining over it. It was a great experience on the very first day in Seoul.
|Seoul at night, taken inside the taxi to Kimchee Guesthouse.
After taking a little rest and praying, my brother and I went out to Itaewon for dinner. I ate bread in the morning and spent the whole time on the flight being stingy about having to pay for my food, so I was a little famished at that moment. Because of the incident about the hostel, we went out a little late and Murree Muslim Food Restaurant was about to close when we arrived. Since our tummies were basically growling at that moment, we just stopped at the next halal restaurant we saw and had chicken rice for our dinner.
After dinner, my brother wanted to visit World Cup Stadium while I decided to just go back to the hostel and took a rest. However, since it was a hostel I didn't research before, I didn't note the number of the subway exit I should be taking to go to the hostel. You see, the hostel was very close to Chungjeongno subway station, like 20 steps away, only if you go through the right exit. There were 10 exits in the station and I went through 8 of them before I found the right one to my hostel. For those going to Seoul, be alert of the number of exit you should be taking. Because some stations have a lot of exits, even more than 10, and it'd be time and energy consuming if you don't know which one you should be taking.
|Me and the infamous banana milk. Pardon my sleepy eyes.
1. Know why you are going to Seoul. For the food? For the culture and history? For the shopping? For the nature? For the Hallyu wave? That way you are able to make an itinerary according to your preference, because trust me, there are a lot of different things you can do and experience in the city of Seoul.
2. Download MetroidHD app in your smartphone. It provides information about the subway; which one to take, how much will it cost, how much time will it take.
3. Choose a hostel near the place you'll frequent, or in the centre which will be near to everything. I chose a hostel near Itaewon, because I wanted to be close to halal food. Because of the change in the hostel, we lived a little far from Itaewon but it was still in the centre.
4. I suggest you buy a T-Money or any other card of the same function, because it is cheaper than having to pay separately for every destination you are going, and just more efficient.
...to be continued...


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Teri was born and raised in Los Angeles and studied Communications and Business at University of Southern California. Upon graduation she joined the sales staff at Rykoff-Sexton (which is now U.S. Foods) and worked with numerous chefs while calling on restaurants, hotels, country clubs and caterers throughout Southern California. In 1997, Teri joined Chocolate Creations, a start up Chocolatier in Los Angeles, securing real estate and setting up a commercial kitchen. For 10 years, as the Director of Sales, she drove the food service, retail and corporate sales, working with accounts such as; Universal Studios, Paramount Pictures, The Cheesecake Factory, Costco and the U.S. White House. Later she opened, The Chocolate Box, a chocolate retail store and manufacturing kitchen in Pasadena. For several years, she also worked at Prudential, selling residential and commercial properties in the Pasadena area working with both buyers and sellers, with sales in the multi-millions. Teri has an entrepreneurial spirit and a history of working with startup companies as well as national accounts. She is currently an active member in the USC Trojan Food Network.Please view Teri's listings at www.restaurantrealty.com
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American Lawnmower Deluxe Light Push Reel Mower
This is my mower. And it works extremely well. Has for over ten years. Even when the dog tries to attack it. Not necessarily for you if you have five or six acres of lawn to cut. But if you have a back yard just large enough for a party of four, then this thing will do you round the bend and back.
if you have a yard - why not try giving up the gas lawnmower and get this nifty reel mower - plus i am sure a little extra cardio wouldn't be that bad.Tags: green, gardening, eco, sustainable, Garden, lawnmower, yard, mowers, reel mowerAdded 8 Years Ago from Cleanairgardening


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23-Dec-2015 - If 'Love is the drug' should Roxy Music be subject to regulatory scrutiny? Can The Verve say 'The drugs don't Work' without conducting a clinical trial? We explore the harmonious...
22-Dec-2015 - GSK has temporarily halted production of Ventolin HFA inhalers at its plant in Zebulon, North Carolina while it investigates customer complaints.
22-Dec-2015 - The US FDA has warned Sun Pharmaceutical Industries about its facility in Halol, India just months after it withdrew approval for an epilepsy drug made at the site.
21-Dec-2015 - Almost half of all manufacturers have reported production downtime resulting from barcode labelling problems, according to a new survey.
18-Dec-2015 - 'Twas the week before Christmas, and what do you know?
17-Dec-2015 - The addition of Xcelience and Powdersize will strengthen Capsugel's position in the dosage forms solutions market, according to the company.
17-Dec-2015 - Under new legislation, the FDA and NIH will receive discretionary funding to combat antibiotic resistant bacteria and advance prevision medicine initiatives.
16-Dec-2015 - Nasdaq-bound Chinese biopharma BeiGene has chosen GE Healthcare's Flexfactory platform for the monoclonal antibody (mAb) plant it is building in Suzhou.
16-Dec-2015 - Data falsification at API facilities is getting harder to spot say regulators who have called for more systems training and international collaboration.
16-Dec-2015 - The FDA is seeking comment on a draft guidance which outlines the administration's thoughts on what constitutes a manufacturing site change.
15-Dec-2015 - AstraZeneca has invested £75m ($114m) to build packaging and warehouse facilities at its manufacturing site in Macclesfield, UK.
15-Dec-2015 - Sun Pharma has announced it will divest a plant in Ohio to further consolidate manufacturing operations following its $4bn acquisition of Ranbaxy.
15-Dec-2015 - Protea Biosciences has teamed up with Agilent Technologies to offer drugmakers mass spectrometry alternatives to UV detection in QA/QC of recombinant proteins.
14-Dec-2015 - If even the apparently non-joke, potential US presidential candidate Donald Trump criticizes your behaviour, you must know what you are doing is unpopular.
14-Dec-2015 - Beat In-Albon, the man tasked with improving profitability at Lonza's manufacturing site in Visp, Switzerland, will step down at the end of the year.
14-Dec-2015 - Trade agreement strengthens enforcement and is important to pharmaceutical industry, says expert.
14-Dec-2015 - The US FDA has alerted compounders of potential particulate matter in an API sourced from Chinese drugmaker Taizhou Xinyou Pharmaceutical & Chemical.
11-Dec-2015 - Sanofi Pasteur says Mexican approval for Dengvaxia vindicates the 'industrial risk' it took when it invested €350m in a dengue fever vaccine plant in 2009.
11-Dec-2015 - DuPont and The Dow Chemical Company have ended speculation by announcing they are to merge and the combined company will be called DowDuPont.
11-Dec-2015 - Scientists have identified seaweed as a source of nutrients for the expansion of pluripotent stem cells needed for future drug testing.
10-Dec-2015 - A new system will simplify and speed-up US imports of pharma ingredients the FDA says, but industry needs to be on board by the end of February.
10-Dec-2015 - The US FDA has approved a chewable, long-acting ADHD drug that was developed for Pfizer by formulation specialist Tris Pharma.
09-Dec-2015 - Early and efficient communication is the key to cutting approval times says the US FDA, which published a draft best practices guide for drug firms this week.
09-Dec-2015 - New Delhi industry group ASSOCHAM says international criticism of Indian drug and API plants is due to a 'severe shortage' of regulatory inspectors.
09-Dec-2015 - Sanofi's recent decision to stop making anti-venom could lead to shortages from next year and place the lives of thousands of people in jeopardy, says the World Health Organisation (WHO).
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Robotics Clubs:
- ChiBots – The Chicago-Area Robotics Clubs
- NIU Robotics – The Northern Illinois University Robotics Club
- Robomo – The St. Louis Area Robotics and Electronics Club
- North American Robotics Association – Promotes mini-sumo robotics in educational and club environments.
General Robotics Suppliers:
- Parallax – Offers Numerous Robotic Kits, Components, and Helpful Information and Resources for all Projects.
- RobotShop – One of the Leading Source's for Domestic and Professional Robot Technology.
- Jameco Robot Store – Large Supplier of Robotic Kits, Parts & More.
- Trossen Robotics – Distributor of Robot Components, Electronic Hardware, Educational Material, and also Features a Popular Forum.
- Pololu – Offers a Wide Selection of Robot Kits, Mechanical Parts, and Electronics.
- HVW Technologies – Offers Numerous Robotics Components, Sensors, Electronics, Tools, and more…
- Fingertech Robotics – A design and manufacturing company that produces custom purpose-built electronics, mechanical parts, hardware, and accessories for hobby robotics.
- Robotics Connection – High Quality Programmable Robot Kits, Components, and Hardware.
- IFI Robotics – Supplier of Large Robot Parts and Electronics Primarily used for FIRST Robotics.
- Innovative RC – Electronic Development and Prototyping Products for Engineers, Hobbyists, and Educational Programs.
- Zagros Robotics – Excellent selection of parts for RC robotics.
- AMain Performance Hobbies – Supplier of many brands of RC planes, boats, cars, trucks, drones, helicopters and rock crawlers.
- Actuonix – Manufacturers of micro-linear actuators that are ideal for robotics applications.
- Solarbotics – Kits for any skill level and supplies to build your own designs as well.
Robot Kits:
- Lynxmotion – Huge Selection of Robot Platforms Ranging from Wheeled Rovers to 6 Legged Walking Hexapods to Robotic Arms
- ROBOTIS – Specializes in Personal Robotics Ranging from Beginner-Level Toys to Advanced Humanoids
- OwiRobots – Distributor of Various Robot Kits and Toys
- Sparkfun – Offers a Large Selection of Electronics for Robotics Applications or Anything Else
- Dimension Engineering – Specializes in Robot Power Electronics, Radio Control Equipment, and More
- Robot Power – Economical Motor and Motion Control Solutions for Many Applications
- Adafruit – Electronics Hobby Supplier
- Solutions Cubed – Produces Versatile and Affordable Engineering Modules
- Digilent – Suppliers of Programming Design Kits for Learning and Research Applications
- LabJack – USB/Ethernet Based Measurement and Automation Devices
- New Micros, Inc. – Robotic Platforms and Embedded Microprocessor System Components
- SchmartBoard – Offers several items that make hand soldering SMT parts fast, easy, and flawless
- Ultrasonic Sensors from MaxBotix – Leading supplier of ultrasonic rangefinders
MicroControllers & Development Kits:
- A-WIT Technologies, Inc. – Supplier of C Stamp Products and other Electronics
- Basic Micro – Makers of the Basic Atom Microcontroller
- MicroEngineering Labs, Inc. – Specializes in Development Tools for Microchip PIC Microcontrollers
Robotics Magazines/Books/News:
- BotJunkie – Collection of Stories and Articles about Recent Robotics Announcements and News
- Nuts and Volts Magazine – Popular magazine for Electronics Hobbyists
- Robot Magazine – The Latest in Hobby, Science, and Consumer Robotics
- No Starch Press – Collection of Books about various "Geek Topics"
- Square 1 Electronics – Offers Several Books about PIC Microcontrollers with Hands-On, Step-by-Step Experiments
- Smart-machines – Artificial Intelligence (AI) and Robotics News
- AMain Performance Hobbies BLOG – An excellent blog for RC robotics.
- Make – An excellent source of DIY projects, ideas, products, and a magazine for makers of all types.
Robotic Combat:
- Robot Fighting League Forum – Online Forum for Robotic Combat Competitors and Beginners
- Standardized Procedures for the Advancement of Robotic Combat – Popular Online Forum for Robotic Combat Competitors With Good Resources
- The Robot Marketplace – Large Supplier of Robot Components Specializing in Robotic Combat Applications
- Inertia Labs – Small Robot Kits and Parts
Mechanical Components:
- BaneBots – Large Selection of Gearmotors Offered in Many Different Reduction Ratios
- AndyMark – Specializes in a Large Variety of Mechanical Components for FIRST Robotics
- ServoCity – Radio Control Electronics and Mechanical Components
- Duralite Batteries – High Performance Lithium Batteries, Chargers, and Accessories
- SR Batteries – Supplier of Batteries for RC and Custom Applications
- RC Judge – Good article on battery chargers. The rest of the site has reviews on all types of RC toys
- PROBOTIX – Supplier of affordable CNC routers, CNC stepper motor driver systems, and more
- Sherline – Manufacturer of Miniature Lathes, Vertical Milling Machines, and Machining Accessories
- Fastener Express – Offers a Large Variety of Miniature Hobby Screws in Metric and Standard Sizes
LEGO Robotics:
- LEGO Mindstorms – Very Popular Robotics Kits Built From Traditional LEGOs (Great for Beginners or Experts)
- Mindsensors – Supplier of Custom Sensors and Interfaces for LEGO Mindstorms NXT, RCX Robotics kits, and FIRST VEX Kits
Commercial/Research/Industrial/Toy Robots:
- Pleo – Advanced Robotic Dinosaur
- WoWee – Various Robotic Toys and Personal Robots
- Tamiya – Offers a Large Variety of Mechanical Models and Accessories
- iRobot – Leading Manufacturer of Robotic Vacuums and Robots for other Applications such as Government and Industrial Applications
- Dr. Robot – Large Selection of High Quality Robot Platforms and other Robotics Components


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Hangout Bridges: Bridges to Peace 2014 (Eng)
visit: hangout-bridges.com In 2014 Google, in collaboration with the Peres Center for Peace and the ORT school network brought 240 students and 17 teachers to empower and educate tomorrow's leaders of the importance of coexistence in the Israeli society. Through the Google+ Hangouts platform, groups of students hailing from the Jewish, Arab, and Druze Israeli sectors, worked together on joint projects that would express the significance of coexistence for the Israeli society. The Hangouts led to face-to-face meetings at the Peres Center for Peace in Jaffa, Israel. The video tells the story of 40 students from Ort Darski Akko (Jewish) and Ort Hilmi Shafi Akko (Arab) high schools, who designed a walking trail in the city that seeks to uncover the rich Jewish-Arab history in ancient Akko, and through that, discover ways to implement this cooperation in the future. This way, each community can tour the other's neighborhoods and be exposed to their peers' city from their perspective.
System Ali - Ala Bali סיסטם עאלי- עלא באלי
פסטיבל יערות מנשה 2012 צילום ועריכה: ריינבו הפקות http://www.rainbowpro.co.il/ http://www.yaarot.com/


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Average City Prices
- Per NightCity
- US$23.81 Brest
Other Locations in Belarus
Check out the Hostelworld.com selection of apartments in Belarus. Read customer reviews of Belarus holiday apartments and view apartments on map of Belarus. Hostelworld.com - the definitive guide to Belarus apartments.
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Very reasonable price for a spacious, clean room. Great staff and nice pool area. Appreciated the fact that you could get food 24/7 from their market menu. It definitely beats similarly priced hotels in the area. I am always extremely pleased with hyatt places and only wish I had discovered them sooner.
- Reservation Options:
- TripAdvisor is proud to partner with Booking.com, Hyatt Hotels Corporation, Expedia, Hotels.com, Orbitz, Travelocity, TripOnline SA, Priceline, Cancelon, Hotwire, Cheap Tickets and getaroom.com so you can book your Hyatt Place Windward Parkway reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Hyatt Place Windward Parkway Hotel Alpharetta
- Hyatt Place Alpharetta
- Hyatt Alpharetta
- Hyatt Place Hotel Alpharetta
- Alpharetta Hyatt


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Clue: Body work?
We have 2 answers for the clue Body work?. See the results below.
Possible Answers:
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- What John Scopes taught
- Cutting class?
- With 42-Across, what people who study living creatures are?
- Frog-dissection class
- High school science course
- Dissection class
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- LA Times Sunday - December 28, 2014
- New York Times - February 08, 2004
- New York Times - October 22, 2000
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