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NeedyBee Mauve Double Deck Hair Clip Hair Pin(Pack of Two)for girls/Babies/Kids(Kids Hair Accessories)
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Buy this cute light purple / mauve grosgrain ribbon double deck hair clip for infant indian baby girls (Pack of Two) by NeedyBee. Have this cute baby hair clip ordered for your infant and toddler from anywhere in India. The classy white colored hair clip comes in rectangle shaped. Buy online this cute hair accessory for your young toddler. Dimension: Hair Clip 1.8"" Material: Grosgrain Ribbon and Alligator Hair Clip Recommended Age: Newborn to 8 Years Color: Light Purple / Mauve Sales Package: 2 Hair Clip Weight: 7 gms All of our designs are a one of a kind with your princess in mind. Your goodies will be wrapped in tissue paper and shipped right to your front door. Each ribbon used has been heat sealed to prevent fraying.We use the best materials. Other Multi Purpose Uses of our clips To wrap Gifts (as bows) Use as an accessory on your bag or sandal use as a decoration while serving food Use as an invitation accessory to make your invitation unique To attach on simple headbands


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Model Mayhem Admin
Jamie WaldenModel Female Birmingham, Alabama, US
My Website: www.myspace.com/liberaljamie
Mayhem # 630621
About Me
I was raised in Alabama, which tends to surprise and amuse people. I'm a college student and an aspiring writer. I'm fortunate enough to have a wonderful family and an amazing boyfriend. Modeling definitely gives me an outlet for artistic expression, but it also provides me with a real adrenaline rush and a boost of confidence. I can appreciate various styles, but I really enjoy edgy work. I'm usually down for just about anything (time permitting, of course). However, I'm not interested in any nude work at this time.
Have you worked with Jamie Walden? Add Credits for Jamie Walden! >>
Credit Notes
2007 - Alcatel Promotional Party
2008 - Puma Runway Show
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Now I know some people absolutely detest Valentines Day whether their single or not but I decided I'd embrace it this year and make a special effort.
I do love my sweet treat - I don't have them often anymore so every once in a while is good, especially for a special occasion such as Valentines Day.
The other evening my friend and I decided we'd give ourselves a challenge by making macaroons.... I know I created the extremely tricky cake from Holly Bell off The Great British Bake Off, but these macaroons were going to be hard work!
"The Rose Water is the key ingredient for these macaroons. Sweet and fragrant, it is an elegant steam distillate of rose petals. Its delicate floral notes are perfect in Middle Eastern, Indian and Greek cuisine and offer a wonderful accent to delicate French pastry glazes and creams. The ideal accessory to compliment the upcoming trend of floral inspired baking."
1. So we got all out ingredients together and began making the Macaroons. We started off by mixing the ground almonds in the food processor for around 30 second, we then sifted it to make sure it was as fine as possible. Then we sft the golden icing sugar into the ground almonds.
2. Measure 60g of egg whites and stir them into the almonds and icing sugar with the rose extract and colouring to make a thick paste.
3. After that you need to measure another 60g of egg whites into the bowl of your electric mixer - pop this to one side.
4. Now you need to place 50ml of water and the golden caster sugar into a small saucepan and heat. Bring this to the boil and cook until it reaches 118c. Once you've done this remove the pan from the heat and cool in a bowl of water.
5.Whisk the egg whites into soft peaks, gradually add the syrup and lightly fold. Whisk on high and until the mixture is thick and stands in firm peaks. After this fold the mixture into the ground almonds.
6.Heat the oven to 170c/Gas Mark 3. Line three baking sheets with baking paper and pipe the mixture into rounds about the size of a 10p piece. Make sure you leave them at room temperature for 30mins before baking.
8. Place the cream and white chocolate in a small saucepan and heat until melted and creamy smooth. I added some pink colouring to make them a little more romantic - that's your choice. Leave this to cool then use it to sandwich your macaroons together!
My first ever attempt at making macaroons was actually quite tough, I will be making another batch before Valentine's Day... so some advice give them a test first then making you romantic batch.


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The modulation of protein–protein interactions (PPIs) by small drug-like molecules is a relatively new area of research and has opened up new opportunities in drug discovery. However, the progress made in this area is limited to a handful of known cases of small molecules that target specific diseases. With the increasing availability of protein structure complexes, it is highly important to devise strategies exploiting homologous structure space on a large scale for discovering putative PPIs that could be attractive drug targets. Here, we propose a scheme that allows performing large-scale screening of all protein complexes and finding putative small-molecule and/or peptide binding sites overlapping with protein–protein binding sites (so-called "multibinding sites"). We find more than 600 nonredundant proteins from 60 protein families with multibinding sites. Moreover, we show that the multibinding sites are mostly observed in transient complexes, largely overlap with the binding hotspots and are more evolutionarily conserved than other interface sites. We investigate possible mechanisms of how small molecules may modulate protein–protein binding and discuss examples of new candidates for drug design.
Protein–protein interactions (PPIs) play a key role in numerous biological processes such as cell proliferation, growth, differentiation, signal transduction and apoptosis; moreover, it has been shown that PPIs are disrupted in many diseases including cancer.1,2 This suggests the attractive possibility of manipulating PPIs for therapeutic intervention. However, targeting PPIs is more challenging than traditional drug discovery that, for example, designs small molecules to bind to enzyme active sites. The complications arise from the fact that PPI interfaces are relatively large, less conserved, often flat or more shallow and featureless in contrast with ligand binding pockets.3–7 Presently, there are small-molecule drugs known to affect about 1% of human proteins,8 and 10–15% of all human proteins are considered "druggable".9 The historical record of drug design and discovery has given rise to the idea that PPIs are much more intractable with respect to small-molecule drug discovery.8 Indeed, for therapeutic use, the chemicals or drugs should be small enough to get inside the cell and also be able to affect the large and often shallow PPI interaction sites.
Nevertheless, there have been a number of studies that suggest targeting PPIs for treatments for some human diseases,10–21 showing that protein–protein interfaces or regions near interfaces might be inherently flexible or intrinsically disordered allowing a small molecule to penetrate these complexes and displace the protein interaction partner.22–24 Several papers review the progress made in this research area.8,25–29 Additional evidence that small molecules do not have to cover the entire protein–protein binding interface but rather target only a small number of interface residues, the so-called "binding hotspot" sites, which contribute the most to the binding energy, has been obtained.30
Many approaches so far have focused on discovering druggable PPIs by in silico screening of small-molecule libraries and searching the chemical space of PPI inhibitors. It was found that PPI inhibitors usually represent relatively large rigid small molecules, containing hydrophobic and aromatic groups.4–7 A recent study showed that SVM (support vector machine) kernels can be successfully used to select molecular descriptors for PPI inhibitors, which characterize specific molecular shapes and the presence of a privileged number of aromatic groups.9 Molecular dynamics simulations, drug design and protein docking studies tried to uncover dynamic and physicochemical properties of protein–protein complexes to find those regions and pockets that can be targeted in small-molecule library screenings.31 Recently, a model was proposed to predict the druggability of pharmaceutically important proteins based on the crystal structures of the binding pockets.32 Despite these efforts, the most comprehensive list of known structure complexes with small molecules disrupting protein–protein interfaces is still very limited [27 Protein Data Bank (PDB) complexes in total]26 and is represented by only eight protein families. More recently, a database with an extensive analysis of these known protein–protein interfaces was made available.33
It is clear that the systematic and large-scale analysis of experimentally observed protein–small-molecule and protein–protein complexes is needed to discover potential protein–protein interfaces that, at the same time, have tendencies to bind small molecules. Such an approach has been undertaken recently by looking for homologous complexes in a protein structure database with overlapping protein–protein and protein–small-molecule binding sites.34,35 This study demonstrated that sampling of the space of homologs is an extremely useful and encouraging approach that not only allowed the recovery of known interaction modulators but also provided a list of potential drug targets. However, a large source of error might come from including complexes that are the result of crystal packing interactions. In this regard, specific methods have been developed to predict and confirm the biological relevance of specific interfaces in crystal structures.36,37 Another source of annotation errors comes from inferring that functions and interactions from distant homologs are common descents and do not necessarily imply similarity in function or interactions and that annotations transferred from one protein to a homolog may result in incorrect functional or interolog assignment at larger evolutionary distances.38 In the current study, we used the homology inference approach and used our recently developed IBIS (Inferred Biomolecular Interaction Server) method39,40 to find those protein complexes that can potentially be targeted by small molecules. To ensure biological relevance of binding sites to the query, IBIS clusters similar binding sites found in homologous proteins based on their sequence and structure conservation, further validates them using various approaches and finally ranks binding sites to assess how well they match the query.
We look for those sites that bind both proteins and small molecules and define them as "multibinding sites". We ask what thermodynamic and structural properties of protein complexes make them more targetable by small molecules. We find that small molecules have a tendency to bind to hotspot residues and preferentially target weaker and more transient protein–protein interfaces. Moreover, we show that multibinding sites are more conserved than the rest of the interface. From the most recent update of the protein structure databank (Research Collaboratory for Structural Bioinformatics PDB41), we compile a nonredundant set of potential PPI interfaces from 642 proteins representing 60 protein families, with strong evidence of multibinding and potential properties of small-molecule PPI inhibitors.
Protein complexes from the current release of the MMDB,55 an automatically parsed and validated derivative of the Research Collaboratory for Structural Bioinformatics PDB,41 are used in this study. PPIs are identified and analyzed at the domain level. The domain assignment is performed by searching the protein sequence against a comprehensive collection of domain models in the CDD.56 PPIs are recorded between different functional domains in the same chain or between different chains from a protein complex.
Protein–small-molecule and protein–peptide interactions are defined for a complete protein chain regardless of its domain annotations. Peptide is defined as a segment of polypeptide chain of 20 amino acids or fewer. Both protein–protein and protein–peptide interactions are considered as PPIs in this study. For small molecules bound to multiple chains, the interaction is assigned to the chain with dominant contacts (>75% of the contacts); otherwise, each protein interaction with the small molecule is recorded separately. An interaction is defined if a protein domain/chain has at least five residues in contact with another protein, small molecule or peptide, and two residues are said to be in contact if any of the heavy-atom interatomic distances is smaller than 4 Å. The "binding site" refers to a group of residues that make a contact with an interaction partner.
We have used the IBIS method described earlier39,40 to analyze experimentally observed complexes and at the same time infer interaction partners and binding sites in proteins without known complexes by inspecting homologs with known interactions. For a given query protein, IBIS collects all its homologs with known structures of complexes from MMDB that have significant structural similarity and at least 30% sequence identity to the query as calculated from the structure–structure VAST57 alignment. IBIS then clusters binding sites using a complete-linkage clustering algorithm. Binding site similarity is assessed based on the structural alignment using similarity scores. At the end of this step, a list of all inferred binding site clusters and binding partners (chain/domains, small molecules and peptides) is compiled, which is derived from homologous structural complexes. We refer to these inferred binding site clusters as inferred binding sites. For each interaction type (protein–protein, protein–small molecule and protein–peptide), all binding site clusters are ranked in terms of their biological relevance and similarity to the query. The components of the ranking score include the sequence PSSM score, the average sequence identity between the query and cluster members calculated over the whole structure–structure alignment and the number of interfacial contacts and the average sequence conservation of binding site alignment columns. The binding site clusters that contain the observed interactions of the query are regarded as observed binding sites, and the rest are defined as inferred binding sites coming from the homologs.
In addition to the ranking scheme, which aims to rank the inferred binding sites using evolutionary relatedness with respect to the query protein, we used other sources. In the case of protein–small-molecule interactions, small molecules were all validated and standardized by the PubChem database,58 which often provides extensive information on their known biological activities. Small molecules with less than five heavy atoms and/or having a molecular mass outside the range of 70–800 Da were ignored in this study. We also excluded nonbiological small molecules based on the list used in our previous study.40 A small-molecule-inferred binding site is deemed nonbiological if all the bound small molecules in an inferred binding site are nonbiological.
Likewise for PPIs, the oligomeric states and binding interfaces were verified using PISA algorithm,36 which identifies biologically relevant interfaces present in crystal structures. If all interfaces in an inferred PPI binding site are invalid according to PISA, the site is deemed nonbiological.
To detect those sites that can bind to both proteins/peptides and small molecules, for a given protein with known structure, we extract all IBIS-inferred sites interacting with other proteins, peptides and small molecules. An inferred binding site is a union of the observed binding site residues of the members of an inferred binding site cluster. The overlap score between protein–protein binding sites and protein–small-molecule binding sites is calculated as:
where Na is the number of residues in protein–protein binding site "a", Nb is the number of residues in the protein–small-molecule binding site "b" and Nab is the number of residues in the intersection of the binding sites "a" and "b", that is, the number of multibinding residues (as indexed with respect to the query). The multibinding sites are defined as those having an SC score greater than 0.5.
Supplementary materials related to this article can be found online at doi:10.1016/j.jmb.2011.12.026
Currently, a total of 239,395 protein chains/domains from 61,413 protein structures are present in IBIS with at least one type of interaction either observed in their structural complexes or inferred from their homologs†. Our method allows analyzing the mechanisms of how a small molecule competes with a natural protein partner.
First, we focused on those cases where protein–protein and protein–small-molecule complexes are available as separate structures in the structure databases. Some of the known examples are shown in Fig. 1 with the structure superpositions of the two different complexes of the same protein. We first tested if our method can recover known examples from the literature of PPIs that are modulated by small molecules. The method successfully recovered six out of eight known cases (Table SM1 and Fig. 1). The interaction between IL2 and IL2-alpha receptor was not recovered because of a partial Conserved Domain Database (CDD) domain mapping (see Materials and Methods). The case of tumor necrosis factor-alpha trimer dissociation mediated by a small molecule has been missed due to the stringent overlap threshold used in our method. In a survey of all the observed PPI and small-molecule interactions in the current PDB, 3223 domains/chains were found to have their observed PPI interfaces overlap with observed or inferred small-molecule binding sites; on the other hand, 4532 protein domains/chains have their observed small-molecule binding sites overlapping with observed or inferred PPI interfaces.
The same protein may be represented in multiple structures solved under different conditions or with mutations and/or in complex with several different small molecules. To account for this, we inferred PPI from close homologs with more than 90% sequence identity and found 6255 chains/domains in PDB with multibinding interfaces. A few examples are presented in Table 1, and the complete list can be accessed from the Web page provided in Supplementary Information.
For each protein chain in PDB/Molecular Modeling Database (MMDB), we assembled a comprehensive list of inferred protein–protein and protein–small-molecule binding sites using IBIS. Since inferred binding sites represent the consensus of binding sites from close homologs, their location reflects the conformational diversity and variability of homologous complexes and at the same time differences in the sizes of small molecules. A total of 27,340 protein chains from 501 CDD families have been determined to contain inferred multibinding sites, and a nonredundant set of 642 chains (culled at 50% sequence identity) from 60 families was compiled with multibinding interfaces that are biologically relevant according to the PISA (Protein Interfaces, Surfaces, and Assemblies server) algorithm.36
Although establishing the biological validity of each of these multibinding sites still requires experimental verification, these sites might be used as starting points to target small-molecule PPI inhibitors. We provide additional annotation for the multibinding sites including their PISA status, biological relevance of small molecules or verification of small molecules using DrugBank42 in Supplementary Materials. For example, of the 642 protein chains, about 400 have at least one multibinding site in which the bound small molecule is also biological.
It should be mentioned that protein structures in PDB often contain additives, detergents and other types of substances used for crystallization. These are not true biological ligands, but they can sometimes be difficult to distinguish from the biologically relevant ones. In the current study, we distinguish the most common biological ligands (see Supplementary Materials). Indeed, the crystallization agents, if present, may sometimes provide additional insights into the binding interfaces on a protein. For example, the nonbiological small molecule in the interface between the E2 protein and E1 helicase of human papillomaviruses probably defines additional regions of the binding pocket that could be exploited to design more potent inhibitors21 (Fig. 1d).
We also note that the PISA program36 is regarded as a state-of-the-art method for the annotation of biological assemblies, with an accuracy estimated at 80–90%. One might expect that the author-determined biological units in the PDB files would be the most reliable. However, it is not difficult to find examples where the annotations in the PDB file are not consistent with the authors' own paper; some authors are quite diligent about this, whereas others may not be so careful. Moreover, an estimate for the accuracy of the author-determined annotations is lacking. Therefore, a highly dependable method such as PISA provides the most consistent results, although researchers should be careful and should consult the relevant literature when accuracy is critical.
We calculated a conservation score using the Shannon entropy with the Henikoff–Henikoff sequence weights for each position in a multibinding site based on the binding site cluster alignments. The highly conserved residues are most likely critical for binding and may well be the binding hotspot residues, namely, those that contribute the most to the binding energy of the protein complex. We analyzed the nonredundant set of protein chains in our data set to check how often the multibinding residues are predicted to be binding hotspots. Hotspot residues were predicted using the PCRPi (Presaging Critical Residues in Protein interfaces) method, which integrates a number of different metrics involving sequence and structure, sequence conservation, "topographical index", computational alanine scanning and others into a probabilistic measure by using Bayesian networks.30 A residue in an inferred binding site is considered as a hotspot if the corresponding residue in the homolog contributing to the site is annotated as a hotpot by PCRPi.
Among the 642 nonredundant protein sequences with biological interfaces and multibinding sites, 259 chains had at least one hotspot on their multibinding inferred site. We found that the association between multibinding sites and hotspots is statistically significant (χ2 p-value 0.01) (Fig. 2), which points to the critical role of binding hotspots in modulating PPIs by small molecules.
At the same time, the analysis of residues in the protein–protein interfaces showed that multibinding residues are more evolutionarily conserved than the rest of the interface (χ2 p-value 0.01) (Fig. 3), and hotspots on multibinding interfaces are more conserved than the rest of multibinding interface (χ2 p-value 0.01). Previously, it was shown that binding hotspots are more evolutionarily conserved than the rest of the interface.43
We mention that small-molecule binding sites can overlap protein–protein binding sites for different functional reasons. First, small molecules can represent natural substrates of enzymes that, in turn, can be inhibited through the mechanism of competitive binding by other proteins. A second group consists of all other cases where small molecules modulate PPIs. Automatically, classifying a small molecule as a native substrate is a challenging task, and there is no such information provided in the PDB files. Therefore, we used a data source from the previous study,44 which compared the PDB ligands to small molecules from the ENZYME and KEGG databases using graph matching algorithms to assess the chemical similarity between small molecules. We mapped these native/cognate ligand annotations to inferred binding sites in IBIS. A total of 108 proteins from the nonredundant set of 642 proteins were found to have at least one multibinding site for a cognate ligand/native substrate. The analysis of these multibinding sites with native substrates in these 108 proteins showed a similar trend of multibinding residues to be more conserved and to have a higher tendency to include binding hotspots (χ2 p-value 0.01). However, the first group of enzyme–substrate complexes showed a lower hotspot frequency but a higher evolutionary conservation of multibinding residues compared to the second group of multibinding interfaces.
The functional enrichment of the nonredundant set of 642 multibinding proteins has been assessed using a selected list (GO slim) of gene ontology (GO)45 functional terms. We define this as a study group—a set of multibinding proteins found in PDB from our study—and a population group—all of the proteins in PDB. The frequency of annotation to a GO term for the study group is then compared to the overall population, which compensates for the functional bias in the PDB. We used Ontologizer,46 which performs a modified Fisher's exact test with correction for multiple testing and also takes the parent–child relationship47 in the GO hierarchy into consideration. GO assignments to PDB entries have been derived from the "gene_association.goa_pdb" gene association file provided by the UniProtKB-GOA group. We found that metabolic, regulation, transducer activity, electron carrier activity and multicellular organismal processes are significantly enriched with multibinding proteins (χ2 p-value 0.01) (Fig. 4). An analysis based on the assigned Enzyme Commission48 numbers of the nonredundant set of multibinding proteins and the rest of the proteins in PDB showed that multibinding sites are significantly overrepresented in enzymes (χ2 p-value 0.01), that is, protein chains with assigned Enzyme Commission numbers.
We checked the hypothesis whether the ability of small molecules to modulate and inhibit PPIs will depend on the stability of protein–protein complexes. We estimated the free energy of dissociation ΔGdiss by using the PISA algorithm. We compared the stability of dimeric complexes containing multibinding sites against all nonredundant dimers in the PDB as determined by PISA. As can be seen in Fig. 5, dimers with multibinding sites are less stable compared to the other dimers (t-test p-value = 0.016). Indeed, proteins with multibinding sites constitute a significantly smaller fraction among permanent protein complexes with the dissociation constant in the nanomolar-to-picomolar range and ΔGdiss of 20–30 kcal/mol or higher.
A majority of multibinding proteins observed in structure databases41 include synthetic small molecules targeting protein inhibitor binding sites. The modulation of PPIs is carried out through disruption, inhibition, stabilization or allosteric regulation. For example, kirromycin antibiotics such as aurodox lock EF-Tu (elongation factor-thermo unstable) in its EF-Tu/GTP conformation, preventing its release from the ribosome, which illustrates the mediation of a PPI by a small molecule. Similarly, the fungal phytotoxin fusicoccin stabilizes the interaction of 14-3-3 with PMA2, a plant proton pump.49 Two more small molecules, pyrrolidone 1 and epibestatin, have recently been found to stabilize the 14-3-3–PMA2 interaction.50 Rapamycin, a potent immunosuppressive drug, mediates the interaction between the human signaling proteins FKBP12 and FRAP that do not normally interact with one another.51 The overlap of the rapamycin binding site (1FAP_A: RAP) with type I TGF-beta receptor in complex with FKBP12 (1B6C_A:B) suggests a possible role of rapamycin in TGF-beta signaling. Indeed, FKBP12 binding to the TGF-beta receptor shields a regulatory segment of the receptor from phosphorylation, which maintains the receptor in its inactive state. Therefore, rapamycin bound to FKBP12 should permit easier activation of the TGF-beta receptor.52
We found that the GTPase (Cdc42) and cell polarity protein (Par6) PDZ domain interface (1NF3_A:C) overlaps with the AMP binding site of a small GTPase Rab1b (3NKV:AMP), suggesting a possible regulation of Par6 PDZ binding to Cdc42 through AMPylation. It has also been shown that mutational disruption of Cdc42–Par6 PDZ coupling leads to inactivation of Par6 in a certain type of epithelial cells.53 Another example is the cresidine binding site of Cu–Zn superoxide dismutase (SOD1) (2WZ0_F:ZZT), which overlaps with the dimer interface of Cu–Zn superoxide dismutase (2NNX_D:A). Recently, this binding site has been annotated as "druggable" for therapeutic purposes for SOD1-associated motor neuron disease.54 A complete list of all the protein chains with observed and inferred multibinding sites is available in Supplementary Materials.
The rapid increase in data on protein sequences and structures is posing new challenges to interpret and use these data productively. While many studies have presented novel methods for functional annotation of these sequences and structures, the annotations in public databases are still error prone or often hypothetical. Even when structural data are available for a particular protein, it can be inconclusive or hard to interpret. Manual curation by an expert, which is the most rigorous and reliable means to legitimize a functional site, is limited by the sheer volume of the data.
Toward addressing this problem, we recently developed an algorithm (IBIS) to analyze and conservatively annotate binding sites in proteins based on knowledge gained via homologous complexes. One important advantage of IBIS-derived sites is that they are weighted based on their recurrence in homologous proteins and ranked using binding site sequence and structure conservation. In the current study, we used the IBIS database to discover many protein–protein interfaces that potentially also bind small molecules. We have found that about 33% of all protein chains/domains with both PPI and small-molecule sites inferred from their homologs have multibinding sites. The likelihood that these sites are biologically relevant is increased by the conservative thresholds built into our method. The GO analysis suggests that multibinding sites are often enriched in metabolic processes. This is also reflected by the significant number of multibinding sites in GO enzyme annotations.
An earlier study showed that those positions, which may bind both small molecules and other proteins, are less conserved compared to monofunctional sites and also exhibit different amino acid propensities.34 However, we found that multibinding sites are more evolutionarily conserved and more likely to contain binding hotspots than other interface positions that are potentially involved in binding of one partner. Moreover, it has been shown previously that hotspots are more conserved than other interface residues,43 and we confirmed this result here with regard to hotspots on multibinding interfaces. Since hotspot residues contribute the most to the binding energy of PPIs, we suggest that binding of small molecules to these positions will have the most disruptive effect on those interactions. It is especially true if these interactions are not very strong. Indeed, as was shown in our study, small molecules mostly bind to transient protein–protein complexes.
It should be mentioned that the apparent conflict between our conservation results for multibinding residues and the results presented previously34 are undoubtedly due to important differences in methodology between the two studies. For instance, the earlier work used SCOP and CATH domain definitions and classifications, which allows for considerably more remotely related proteins and may affect the reliability of homology inference. IBIS uses a conservative threshold for homology inference (at least 30% sequence identity), and we observe that multibinding sites are under stronger evolutionary constraints than even the fairly conserved family background.
Binding of small molecules to proteins and protein complexes could cause a shift of equilibrium in favor of a subset of conformations that has higher or lower preferences to binding another partner. A small molecule may bind at a site far from the protein–protein interface and regulate protein binding through allosteric mechanisms or might bind at or near the protein–protein interface and directly influence the binding. In this study, we focused on the latter case. We showed that small molecules can bind to hotspots and through competitive binding prevent PPI. At the same time, we show examples of small molecules mediating PPIs. Other mechanisms of small molecule binding and their functional roles might be elucidated in future studies when more structural complexes are available.
This work was supported by National Institutes of Health/Department of Health and Human Services (Intramural Research Program of the National Library of Medicine).
†All multibinding sites observed in our study for proteins in the PDB are accessible from http://www.ncbi.nlm.nih.gov/Structure/ibis/P-D/multibinding.html.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's national science library in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text: wow 2016, 2015 was best year 4 card well best yet. it was its first proper year. so here's a jammer, the first for this year, its classic style fried bedroom silly's, chuntering static, bleeps and blops from the black plague, tape woopey garble and slap bangin bedroom acoustics all with pop star croon. do u want more? well if u do go some place else. xxxx


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Cyber Reappers is a Red Dead Redemption posse.
About the posseCyber_Reappers is a clan for players with no sympathy for others. We are the Grim Reappers of the Cyber world and we will take anyone that stands in our way down to the fiery pits of hell. We use any guns provided to us by the game (if they are given to u in the game they are for u to use) so use any weapons u want. Our main goal is to Raise Hell and reign supreme. Stand against us and pay the price, join us and rejoice in the sweet taste of victory.
Joining the posse
To join the clan u need to add Cyber_Murder (that would be me) and on the request announce that u are a potential recruit for Cyber_Reappers. Rules to live by: 1.Never leave teammates alone in times of need unless they are equally ranked and its a one on one fight. 2.Obey the leader and the co-leaders. 3. U must play the game often. 4.Be cool. 5. Make The Reappers proud!
If you are joining u must creat a new psn with the name "Cyber_" in it inorder to make it easier for us to recognize who you are and that you are in Cyber_Reappers.
- Leader 1 Cyber_Murder
- Leader 2 isanate
- Leader 3 GRIMREAPER0993
Other members
- Member 1 russian_97
- Member 2
- Member 3 etc.


text:
Computers are behaving more and more like the human brain, which is why it's now acceptable for children to claim a computer as their legal guardian. The future is now with this Groupon.
The Deal
- $39 for virus removal and computer optimization (a $75 value)
Velocity Computer Tech
It's hard to imagine getting by today without computers. Velocity Computer Tech makes sure no one has to by maintaining and repairing laptops, computers, and tablets. No matter the glitchy technical whatsit hampering memory and motherboards, techs troubleshoot the problem and quickly devise a solution. Services include everything from a basic tune-up and virus removal to a screen replacement to a total rebuild. To ensure clients know which services are necessary before work begins, they offer free computer diagnostics.


text:
Vintage Antique Clothing, Jewelry, Accessories, Textiles, Sewing with that added flair!
Pair Bakelite Buttons Vintage 1940s Art Deco Brown Deeply Carved Shank
Gingham Doll Apron Bonnet Vintage 1930s Mama Doll Green Cotton Lace
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If this doesn't do it for Santorum, we don't know what will. The gaffe does not however looks like to go away soon – it might even cost Rick Santorum the thing he wants most – Presidency of the United States of America.
Rick Santorum was vying for a chance to prove himself as the Republican nominee for the 2012 Presidential elections and took the race to Janesville, Wisconsin. 'This race is about who we are', said Santorum, perhaps unaware of the irony which waited for him.
"There are things that bring us together…there are things that bind us," Santorum said, speaking how despite their differences, Americans shared a common bond. A bond, which he later added, President Obama seeks to retain in order to keep Americans divided. "He takes the opportunity to divide us…to remind us of our differences," Santorum said.
However what happened at 34:25 is likely to resonate with a lot of Americans and would eventually; cost the Presidency hopeful, his dream.
"We know, we know the candidate Barack Obama, what he was like. The anti-war government nig- uh, the uh America was a source for division around the world."
Did Rick Santorum almost use the N word? You be the judge
You can watch the video here, starts at the 34:25 mark:
Read More: Twitter Reacts To Rick Santorum's Obama N-word Gaffe
Some of the spin doctors are saying Santorum was about to say 'negotiator', but from the slip up and the subsequent horrified look, and how Santorum completely gives up the sentence and starts another is a dead giveaway on what he was going to say. There might be no coming back for Rick Santorum after this.
"The lights of freedom may very well go out if we don't win this election," Santorum told the Republican crowd gathered at Janesville, Wisconsin, earlier. Adding in that this election is the most important in the nation's history, completely forgetting that the American history is based on equality and freedom. For Rick Santorum's Presidential dreams, from the looks of this, the light has certainly gone out.


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Pelion Resort, Volos
- Free WiFi and free parking
Main amenities
- 36 guestrooms
- Restaurant and bar/lounge
- Outdoor pool
- Breakfast available
- Sauna
- Spa services
- Business center
- Coffee/tea in a common area
- Air conditioning
- Daily housekeeping
- Garden
- Lobby fireplace
- Free WiFi and free parking
For families
- Free cribs/infant beds
- Playground on site
- Rollaway/extra beds (surcharge)
- Connecting/adjoining rooms available
- Private bathroom
- Separate bedroom
What's around
- In Portaria
- Museum of Folk Art and History of Pelion (2.4 mi / 3.8 km)
- Volos Town Hall (8.2 mi / 13.2 km)
- Volos Port (8.4 mi / 13.5 km)
- Volos Municipal Theatre (8.5 mi / 13.7 km)
- Volos General Hospital (9 mi / 14.5 km)
Pelion Resort, Volos
Hotel Info: 866-539-8117from $62
- Superior Double Room, Fireplace
- Junior Suite (2 People)
- Double Room
- Superior Double Room
- Triple Room
- Suite for 2 people
- Two Bedroom Suite for 4 people
- Double Room (Stay 3 and save)
- Junior Suite (3 People)
- Triple Room 3 nights
- Double Room (1 night free)
- Superior Double Room
- Triple Room (1 night free)
- Superior Double Room (1 night free)
- Standard Double Room, Fireplace
More great choices
At a glance
Key facts
Hotel size
- This hotel has 36 rooms
- This hotel is arranged over 2 floors
- Check-in time 2:00 PM-5:00 PM
- Check-out time is noon
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- One child (2 years old and younger) stays free when occupying the parent or guardian's room, using existing bedding
- No pets or service animals allowed
- Free WiFi and wired Internet access in public areas
- Free WiFi and wired Internet access in rooms
- Free self parking
- Extended parking
Other information
- Resort fee included
Nearby Four Star Volos hotels
In the hotel
- Buffet breakfast (surcharge)
- Restaurant
- Bar/lounge
- Coffee shop/café
- Poolside bar
- Room service (during limited hours)
- Coffee/tea in a common area
- Barbecue grills
- Outdoor seasonal pool
- Outdoor pool
- Spa services on site
- Spa tub
- Pool sun loungers
- Sauna
- Pool umbrellas
- Business center
- Conference space size (feet) - 250
- One meeting room
- Front desk (limited hours)
- Tours/ticket assistance
- Dry cleaning/laundry service
- Laundry facilities
- Luggage storage
- Wedding services
- Multilingual staff
- Number of buildings/towers - 6
- Year Built 2008
- Elevator/lift
- Designated smoking areas
- Garden
- Fireplace in lobby
- Television in common areas
- Accessible bathroom
- In-room accessibility
In the room
- In-room climate control (air conditioning)
- Air conditioning
- Minibar
- Slippers
- Premium bedding
- In-room massage available
- Yard
- Private bathroom
- Bathtub or shower
- Designer toiletries
- Hair dryer
- Satellite TV channels
- MP3 docking station
- DVD player
- Desk
- Free WiFi
- Phone
- Daily housekeeping
- In-room safe
- Connecting/adjoining rooms available
- Access via exterior corridors
Top Volos hotels
Recently reviewed hotels in Volos
- Aleka's HouseFrom
"Our stay was wonderful! The staff was kind and extremely helpful. Everyone was very to our 3 young boys!! The room was clean and comfortable!!"May 12, 2017
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- Palios StathmosFrom
"The building is beautiful and for those arriving on the gorgeous old train, it overlooks the terminal station. The restaurant is great, and the outdoor grill smells wonderful. The…"Oct 10, 2016
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"The hotel is a beautifully renovated 19c house in a picturesque village. The host is unbelievably nice - dedicated and pleasant - he helped with insights about traveling in the…"Aug 19, 2016
Nearby hotels
Also popular with travelers from the USA
Special features
DiningPlateia - This poolside family restaurant specializes in Greek cuisine and serves breakfast, lunch, dinner, and light fare. Guests can enjoy drinks at the bar. A children's menu is available. Open select days.
On Site
- Sauna
- Spa tub
- Playground on site
- Bicycle rentals nearby
- Hiking/biking trails nearby
- Kayaking nearby
- Mountain biking nearby
- Sailing nearby
- Scuba diving nearby
- Ski lessons available nearby
- Ski equipment rental shops nearby
- Downhill skiing nearby
- Snorkeling nearby
- Snowboarding nearby
- Snowmobiling nearby
- Water skiing nearby
Pelion Resort, Volos's small print
Also known as
- Pelion Resort
Cash transactions at this property cannot exceed EUR 500, due to national regulations. For further details, please contact the property using information in the booking confirmation.
The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.
Only registered guests are allowed in the guestrooms.
Some facilities may have restricted access. Guests can contact the property for details using the contact information on the booking confirmation.
This property does not have elevators.
Reservations are required for massage services and can be made by contacting the property before arrival at the number on the booking confirmation.
The name on the credit card used at check-in to pay for incidentals must be the primary name on the guestroom reservation.
Mandatory fees
The charges below are included in the total price displayed:
- Gala dinner fee
- Christmas Eve gala dinner fee for stays on 24 December
- New Year's Eve gala dinner fee for stays on 31 December
- Resort fee
Resort fee inclusions:
- Breakfast
- Beach towels
- In-room safe
- In-room bottled water
- Parking
Inclusions may be listed elsewhere on the page as free or for a surcharge.
Optional extras
Rollaway beds are available for EUR 20 per night
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.
Hotel registry number: 0726K014A152301
Is the description of this hotel not correct? Tell us


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In the recent years, there has been an increase in applications of non-contact diffusion optical tomography. Especially when the objective is the recovery of fluorescence targets. The non-contact acquisition systems with the use of a CCD-camera produce much denser sampled boundary data sets than fibre-based systems. When model-based reconstruction methods are used, that rely on the inversion of a derivative operator, the large number of measurements poses a challenge since the explicit formulation and storage of the Jacobian matrix could be in general not feasible. This problem is aggravated further in applications, where measurements at multiple wavelengths are used. We present a matrix-free model-based reconstruction method, that addresses the problems of large data sets and reduces the computational cost and memory requirements for the reconstruction. The idea behind the matrix-free method is that information about the Jacobian matrix could be available through matrix times vector products so that the creation and storage of big matrices can be avoided. We tested the method for multiple wavelength fluorescence tomography with simulated and experimental data from phantom experiments, and we found substantial benefits in computational times and memory requirements.
© 2009 Optical Society of AmericaFull Article | PDF Article
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Appl. Opt. 51(36) 8731-8744 (2012)
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Biomed. Opt. Express 2(11) 3207-3222 (2011)


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Dear God make it stop. I can hardly believe that Drew Barrymore is associated with this load of tosh,The characters are so weak and wooden .Acting skills are minimal to say the least.
No matter how urgent the situation is they always find time to change clothes, do their hair ,and make up .Come on you women out there how long in the real world does it take you to change outfits and do hair.In my experience waiting for the good lady ,blooming hours .Any way in this episode as with the first episode we have some one in the water soaking wet, and yet within a few minutes of getting on to the boat,yes the hair is bone dry. Like the plot .The continuity people must be blind or just plain useless.
For some of comments i have read about how good the characters are being developed ,I think you must be watching reruns of the original series. This show is made for the under 5,s who enjoy playing dress up .Time this was killed off and they could become real Angels in that garbage pile where they put all failed efforts of puerile rubbish . Enough is enough .Should have been dropped after the pilot..


text:
Caravan to Cape Leveque?
by David Richardson
Is it possible to tow an "off road" caravan to Cape Leveque? We have towed it to Bamaga without drama.
David R
Comments for Caravan to Cape Leveque?
Click here to add your own comments
Join in and write your own page! It's easy to do. How? Simply click here to return to Cape Leveque.
Travelling to the Kimberley?
The free Kimberley Pocket Guide
is a great introduction to travel in the region.
The full Kimberley Travel Guide
shows you how to make the most of your adventure at Australia's last frontier.
Travelling to the Northern Territory?
Destination Top End offers the same comprehensive, detailed insider information for the tropical regions of the Northern Territory.
A must have if you travel to or from Darwin.
NEW! Destination Red Centre is the latest addition in this popular series. Monica Coleman takes you through Australia's red Outback heart, offering all the detail and insider tips that you have come to know and love about our guides. With special emphasis on Aboriginal communities and culture.
A must have if you travel to or from Alice Springs/Uluru.
Return to Kimberley Travel Guide home page


text:
I'm rubbish when it comes to girls. I've never really perked the interest of any woman i've been interested in. Moreover when women have been interested in me I have always failed to notice. For the past year or so I had had a crush on a particular woman, but over the last couple of months I have come to realise our friendship is too important. So for now i'm going to try and ignore the fact that i'm a dysfunctional man incapable of building relationships with women. That is, to temporarily pretend i'm happy on my own, even if in truth i am not. Is that a problem?
I have given up on love for now, is that a problem?
Have an opinion?
What Girls Said 1
No, you're right. Friends are important. Taking a break from dating is good for something. And people sometimes have the chance to focus on the own things than dating.00|00|0
What Guys Said 1
If you were over love you wouldn't be making an announcement so part of you hasn't completely dropped those fantasies haha xD00|00|0
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Author | Sumita Patra | Thursday, Oct 26,2006 7:49 AM
Comma Consulting has been on an account winning spree of late. The communications management firm has added three new businesses to its kitty in the last one month – Alstom India, Spencer Stuart and AT Kearny – who have awarded their PR mandate to Comma.
Alstom India is one of the leaders in power and transport technology, while AT Kearny is among the top-end consulting firms in India. Spencer Stuart, an executive search firm, recently forayed into India. The size of the collective business couldn't be ascertained at the time of filing the report.
The core task before Comma Consulting is to help the clients meet their PR requirements, which includes media relations as well.
Rakesh Thukral, COO, Comma Consulting, said, "All of them require strategic inputs and that is what we aim to provide them in a qualitative manner. The core challenge for us is to get the objective right for the concerned company because a communication objective is of no use if it doesn't meet the business objective."
All three accounts would be serviced from the firm's Delhi and Mumbai offices. Comma is on the lookout for communication professionals to strengthen its staff. The firm is also planning to set up its Bangalore office soon.
Programming HeadRadio Mango


text:
View Talk Edit
|Artwork · Comic-Con · Transcripts
DamonCarltonAndAPolarBear.com is a website first introduced at the Comic Con 2009 Lost panel, when the comedian Paul Scheer handed Damon Lindelof and Carlton Cuse a velvet painting he had been working on for the past 6 months, featuring the duo and a polar bear. It served as the central website to the ARG, presenting 16 different frames and a hub page with clues. All posters are now revealed and sold out.
During the Q&A session in the Comic Con 2009 Lost panel, comedian Paul Scheer presented a velvet painting he has been working on for the past 6 months, featuring executive producers Damon Lindelof and Carlton Cuse, hugged by a polar bear. Paul revealed that more artworks by him will be available on his website, carrying the same name as the painting. Paul rushed to the stage, and handed the painting to Damon.
Ronie Midfew Arts
The website, functioning as a blog, has been running since July 20th, 2009, five days before the Lost panel. The blog posts mainly theories and Lost-related news bits, though starting on August 3rd, mentions of a certain legal firm were made. Later on, news of his painting being thrown away were made, presumably by the company Ronie Midfew Arts (anagram to Widmore Fine Arts). Scheer uploaded a letter sent to DocArzt by the company, claiming Scheer has no right creating Lost-related artworks. The letter was sent by Ronie Midfew's assistant, Alexandra Miller.
On August 12, Scheer recieved a call from Alexandra Miller.
Caller: Hello this is Alexandra Miller from Ronie Midfew Fine Arts.
Me: DON'T TELL ME WHAT I CAN'T DO!
AM: Excuse Me?
Me: You're just trying to be like the unsympathetic outback travel agent in episode four of Season 1, WALKABOUT.
AM: I'm Sorry.
PS: This is destiny, this is destiny… This is my destiny! Hey, I'm supposed to do this, dammit!
AM: Sir, if you could please just calm down. I just wanna talk.
PS: Umm…A small boy once asked me if I was a bad man. If I could answer him now, I would tell him that I once killed a man to save my brother's life….Mr. Eko.
AM: What?
PS: You all ev'rybody!! You all………ev'…ry…….body??" (NOTE: At this point [Scheer] ran out of recognizable Lost quotes and hung up the phone)
Scheer did some internet research, and apparently left some comments on the wrong Alexandra Miller Facebook page. He blamed it on Microsoft's Bing.
Soon afterwards, a messenger arrived at Paul's house, informing him that Ronie Midfew invited him over to his office. During the meeting, Midfew and Scheer came to an agreement regarding the paintings, and along with show-runners Damon and Carlton, they combined their efforts to make the ultimate Lost experience prior to the airing of the final season.
Visit to ABC
Transcript: Damon, Carlton and a Polar Bear Transcripts
On August 7th, Scheer visited ABC studios in Los Angeles in order to uncover the truth behind the trashing of his painting. Unable to get through security, he pretended to be Tom Friendly, which the security man recognized and gave permission to enter. While searching for the building, Scheer revealed he wrote a fanfic on the back of his painting, centering on Adam and Eve. Scheer failed to find the production building, though it was right behind him. Carlton also bumped into Scheer, though Scheer did not recognize him.
Searching through a dumpster outside the Lost offices, Scheer found a rose, relating it to The Bachelor. The rose accidentaly broke, after Scheer presented it to the cameraman and said "will you take this rose?"
Scheer also found a set design of the Temple elevation (set 493: 4+9+3=16), featuring hieroglyphs, four sides and a column. Along with it, Scheer recovered the shredded front page of the premiere episode's script of season 6, which he taped back together and revealed the episode's title.
During his visit, Damon passed by while Scheer was looking through the trash, and while on the phone he remarked "I don't care what his motivation is, just make him dead."
Enhanced Temple sketches
Artwork hunt
Starting on August 16th, Ronie Midfew Arts and Scheer combined their efforts (with the help of executive producers Damon Lindelof and Carlton Cuse), and organize a hunt which eventually will present Lost fans with 16 original paintings. The hunts involve following clues on the main website, leading fans to real-world locations where they will receive a website address to where a new poster is hidden. Events have been organized in California, Boston, New York and Arizona.
- The site is owned by ABC. [1]
- During his visit to ABC, Scheer made a few references to characters and events from Lost episodes.
- He compared his visit to ABC to Ethan's mission of infiltrating the survivors. ("A Tale of Two Cities") ("Raised by Another")
- Walking through the lot with his map, Scheer mentioned it is like the cable Sayid found and followed. ("Solitary")
- While in the car, a drinking cup from Mr. Cluck's Chicken Shack is visible for a few seconds.
- The RSS Feed credits "admin" as the author of the posts.
See also
- Fan events - for the Lost Underground Art Project


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The West Blog
Latest Post
July 24, 2017
Corporate Responsibility Report – Part 3: Celebrating Diversity

In Parts 1 and 2 of this five-part blog series on West's inaugural Corporate Responsibility Report, we focused on Compliance and Ethics Programs, and Philanthropic efforts.

At West, we recognize and value the spectrum of people who make up our Company. It is a key to our success. Below is the third part of this five-part series reviewing West's Diversity measures.

Previous Posts


text: Release Notes: In this release, the VM dependency on Rik's engine was resolved, latency was decreased in several paths, and a tracing bug was fixed so that you can now trace migrated processes. A small race in migration was eliminated, and the user-space tools were totally rewritten for GPL compliance. Some documentation was also added.


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
how do I attach a file to printAsked by 4Hregion1 on March 02, 2015 at 03:00 PM
JotForm Support
There are multiple ways to do it in JotForm
1. Add the Upload field on your JotForm so that it can be printed once uploaded.
2. You also try using any of the 3 widgets below that have upload functionality.
You can check more here http://widgets.jotform.com/search/upload
Please let us know if you need further assistance.


text:
why is it not acceptable for guys to wear women's clothes? I have enjoyed wearing womens panties and other lingerie since I was 11 of course I also like to wear dresses and skirts as wellbut why is it okay for women to wear mens clothes but not meant to wear girls clothes
Most Helpful Guy
I personally find the idea of wearing women's clothing a bit odd and confusing... And that's why *I* am never going to wear women's clothing. Why can't *you* wear women's clothing? I couldn't tell you. It doesn't affect anyone else, so you should really be allowed to do it without anyone attacking or harassing you for it.
If I saw you on the street in a dress, I'd probably smile to my self and be impressed that you had the guts to dress the way you want. But most people are not like me. And some may never be as accepting.
So to answer your question, "why is it not acceptable," I'd have to say some people are just too immature and/or total assholes and they just can't deal with something they don't understand or agree with.10|10|0


text:
Mobile broadband reviews fall into 2 categories. Use the jumplist below to get to what you need instantly:
- Mobile Broadband Reviews (Services)
- Mobile Broadband Devices
Mobile Broadband Service Reviews
Between postpaid, prepaid and rental providers, there's an exhaustive list of reviews to choose from unless you already know which carrier you're checking out. If you don't already know what provider you're interested in, check out:
Otherwise browse through the reviews below:
While postpaid carriers have cheaper equipment, monthly plan costs (if used regularly) and better 3G coverage and better 3G speed, they usually have 2 year contracts. Check out individual reviews as well as comprehensive comparisons.
2010 Wireless Broadband Comparison
2009 Wireless Broadband Comparison
2008 Wireless Broadband Comparison
2010 AT&T Wireless Broadband Review
2009 AT&T Wireless Broadband Review
2008 AT&T Wireless Broadband Review
2010 Sprint Mobile Broadband Review
2009 Sprint Mobile Broadband Review
2008 Sprint Mobile Broadband Review
2010 T-Mobile Wireless Internet Review
2009 T-Mobile Wireless Internet Review
2008 T-Mobile Wireless Internet Review
2010 Verizon Mobile Broadband Review
2009 Verizon Mobile Broadband Review
2008 Verizon Mobile Broadband Review
Prepaid mobile broadband providers have no contracts but can more expensive if used long-term and not occasionally. The exception to this rule is Cricket Broadband that is not only cheaper than most post-paid providers but also contract-free.
2010 Prepaid Mobile Broadband Comparison
2009 Prepaid Mobile Broadband Comparison
AT&T Prepaid Mobile Broadband Review
Verizon Prepaid Mobile Broadband Review
Virgin Mobile Broadband Review
2 year mobile broadband contracts aren't for everyone. Neither is having prepaid wireless broadband hotspots all across the country.
Sometimes, you just need to be able to get online for a couple days while you're at a business conference, on vacation, or RV'ing across the country on a roadtrip. That's where mobile broadband rental comes in.
2010 Mobile Broadband Rental Comparison
2009 Mobile Broadband Rental Comparison
With 6 options to choose from, finding the best broadband device is like buying a new or used car. It boils down to your taste for style and functionality.
Not sure what to get or just new to it all?
Learn How To Pick The Best Broadband Device
Otherwise, check out individual mobile broadband device reviews.
Just as how you've got 3 types of broadband devices, there are also got 3 kinds of broadband cards:
1. USB Broadband Cards
2. ExpressCards
3. PC Broadband Cards (PCMCIA)
If you don't have a specific card in mind, it can be a bit much to go through the exhaustive list below. Instead, check out the Broadband Card Comparison for a condensed version.
2010 Broadband Card Comparison
2009 Broadband Card Comparison
2008 Broadband Card Comparison
Sierra USBConnect Lightning 305 AirCard
Sierra Wireless 598U Broadband Card
Novatel Merlin C777 2-in1- ExpressCard/PC Card
Novatel Ovation U760 USB Broadband Card
Novatel S720 PC Broadband Card
Sprint U301 3G / 4G WiMAX Card
Novatel Ovation USB760 Broadband Card
Retired Models:
- Option GT Ultra
- Option GT Ultra ExpressCard
- Sierra Wireless 881 AirCard
- Sierra Wireless Mercury USB AirCard
- Novatel Ovation U727 USB Broadband Card
- Sierra Wireless AC597E ExpressCard
- Novatel EX720 ExpressCard
- Franklin Wireless U680 USB Broadband Card
- Sierra Wireless Compass 597 USB AirCard
- Kyocera KPC680 Broadband ExpressCard
- Novatel Wireless USB720 USB Broadband Card
- Novatel Ovation U727 USB Broadband Card
- Novatel Merlin V740 ExpressCard
- UTStarcom PC5750 PC Broadband Card
- UTStarcom UM175 USB Broadband Card
- UM150 Broadband Card
- Novatel Merlin V740 ExpressCard
Personal Hotspots get mobile broadband via 3G or 4G signals and then broadcast it via WiFi for you and a couple friends to connect to.
3G Netbooks are basically mini laptops that have mobile broadband chips built-in. For you, that means mobile broadband anywhere you've got your laptop.
New to the concept?
Check out:
Why Give A Damn About Mobile Broadband Netbooks?
Otherwise, check out the reviews:


text:
With First Tutors: Music you can find a Wigton double bass teacher. Double Bass lessons are the perfect way to begin an entertaining new hobby, acquire a new skill and gain confidence with double bass. Use our site to find Wigton double bass teachers and choose which matches your needs so you can start your double bass tuition in Wigton immediately!
This is the best place to locate Wigton double bass tutors in the UK, helping you find Wigton double bass lessons close to home and Wigton teachers to help with any instrument like piano, violin or guitar. We can help you find Wigton double bass tuition or a Wigton double bass tutor to prepare for double bass grades and develop your double bass skills! We order Wigton double bass tutors local to you according to their hourly prices and proximity to you.
If you are a double bass teacher and would like to offer double bass tuition services in Wigton or throughout the UK, please register with First Tutors: Music or find out more about us here. Becoming a Wigton double bass tutor is a great way to re-affirm your own skills through giving double bass lessons while helping someone else, all in your local Wigton area!


text:
FREE SHIPPING on orders of $85 or more
An excerpt from www.HouseOfNames.com archives copyright © 2000 - 2017
Norman Conquest brought to England in 1066. The Marckisume family lived in Sussex. Their name, however, is thought to be derived from a location in Normandy called Argenson, which would have been used as a name in its local form, D'Argenson, meaning from Argenson. The location, however, like many small settlements of the time, has been lost to the map in contemporary times. It is likely that the M now appears as the first letter of the name in most cases due to confusion with the similar metronymic name meaning son of Margaret.
Marckisume Early Origins
Sussex where they held a family seat from very early times and were granted lands by Duke William of Normandy, their liege Lord, for their distinguished assistance at the Battle of Hastings in 1066 A.D.
Marckisume Spelling Variations
Marckisume Spelling Variations
It is only in the last few hundred years that the English language has been standardized. For that reason, Anglo-Norman surnames like Marckisume are characterized by many spelling variations. Scribes and monks in the Middle Ages spelled names they sounded, so it is common to find several variations that refer to a single person. As the English language changed and incorporated elements of other European languages such as Norman French and Latin, even literate people regularly changed the spelling of their names. The variations of the name Marckisume include Margesson, Margeson, Margerison, Margetson and many more.
Marckisume Early History
Marckisume Early History
This web page shows only a small excerpt of our Marckisume research. Another 187 words (13 lines of text) are included under the topic Early Marckisume History in all our PDF Extended History products and printed products wherever possible.
Marckisume Early Notables (pre 1700)
Marckisume Early Notables (pre 1700)
More information is included under the topic Early Marckisume Notables in all our PDF Extended History products and printed products wherever possible.
Marckisume In Ireland
Marckisume In Ireland
Some of the Marckisume family moved to Ireland, but this topic is not covered in this excerpt. Another 147 words (10 lines of text) about their life in Ireland is included in all our PDF Extended History products and printed products wherever possible.
The Great Migration
The Great Migration
Faced with the chaos present in England at that time, many English families looked towards the open frontiers of the New World with its opportunities to escape oppression and starvation. People migrated to North America, as well as Australia and Ireland in droves, paying exorbitant rates for passages in cramped, unsafe ships. Many of the settlers did not make the long passage alive, but those who did see the shores of North America were welcomed with great opportunity. Many of the families that came from England went on to make essential contributions to the emerging nations of Canada and the United States. Some of the first immigrants to cross the Atlantic and come to North America carried the name Marckisume, or a variant listed above: Edward Margeson was one of the passengers on the "Mayflower" which arrived in 1620; Robert Margeson settled in Virginia in 1655; Joseph Margerison settled in Philadelphia in 1880..
The motto was originally a war cry or slogan. Mottoes first began to be shown with arms in the 14th and 15th centuries, but were not in general use until the 17th century. Thus the oldest coats of arms generally do not include a motto. Mottoes seldom form part of the grant of arms: Under most heraldic authorities, a motto is an optional component of the coat of arms, and can be added to or changed at will; many families have chosen not to display a motto.
Motto: Loyalité me lie
Motto Translation: Loyality binds me.
Marckisume Family Crest Products
Marckisume Family Crest Products
See Also
See Also
Other References
The Marckisume Family Crest was acquired from the Houseofnames.com archives. The Marckisume Family Crest was drawn according to heraldic standards based on published blazons. We generally include the oldest published family crest once associated with each surname.
This page was last modified on 31 January 2013 at 13:56.
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NextNav closes $70 million Series D funding
NextNav, launched by former XM Satellite Radio CEO Gary Parsons, is a leading provider of location services for indoor and urban environments. The financing will be used to extend the commercialization of NextNav's revolutionary Metropolitan Beacon System ("MBS") positioning network. MBS provides accurate horizontal and vertical location services inside buildings and in urban areas where satellite-based GPS signals aren't available or reliable. NextNav is deploying its MBS network much like a cellular network, resulting in wide-area coverage that delivers its location services to every building within its network footprint.
Hogan Lovells corporate partner Richard Becker from the Northern Virginia office led the team, with assistance from Corporate partner Randy Segal, Antitrust partner Michele Harrington, Corporate associate Gabrielle Witt (all Northern Virginia), and Washington, D.C. Tax partner Shawna Tunnell.


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There's a new suit in town — it's powerful and sexy and is the perfect addition to every working women's wardrobe. We spotted these updated suits on the runways at New York Fashion Week for Fall 2013 at Oscar de la Renta and Jason Wu, and we were instantly sold on the trend with all of its gorgeous iterations. To get the full story on the new suit, watch our NYFW trend report right here. On Allison McNamara: BCBG Max Azria jacket, Zara blouse.
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For sale is a beautiful vintage 18kt yellow gold ladies brooch in the shape of a dragon. The circle pin measures 1" in diameter and has a non-gold white metal pin, hinge and gold "C" clasp to secure it. The brooch contains one round faux half-pearl, it has been die-cast and assembled. This beautiful brooch comes with an insurance appraisal of $346.00. Free shipping in the USA. Note: several lead solders are black in appearance.
5 other shoppers have this item in their Cart or Wish List .
Fine Art & Treasures from Around the World.
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I'm an engineer working and living in Annapolis. School takes me to DC often and friends get me up to Baltimore pretty regularly. I've had a profile on this site for what seems like forever and I've met some great friends through it and some pretty lasting relationships. Here trying to find some more.
Currently work as an engineer which aligns well with what I'm good at. Unfortunately it probably isn't the thing I would love to do, thus the more school.
After finishing one part time masters program while working full time I decided to start another. This time an MBA which I'm actually having a blast working on.
I've been in my house a little over a year now and its finally starting to come together. It took a decent amount of work to get to where it is and I've only got a few more handy man tasks left to complete before I'll be good to go.
After a while people normally think that I'm mature for my age or overly responsible.
-A comfy bed and stuffed animal to use as a pillow
-A few really close friends
-My amazing family
-Iced Tea
-Long showers
-A vacation every now and then


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Alloy USA Axle Shaft Kit (1971-80) International Scout IIs, for Dana 44 Front
This 30-spline chromoly front axle shaft kit from Alloy USA fits 71-80 International Scout IIs with Dana 44 front axle.
Was: $961.99
Sale: $720.99
Save: 25%
Save: $241.00
High tensile strength 4340 Chromoly is heat-treated, tempered and induction hardened to produce these heavy-duty axles that are up to 40% stronger than stock 1040 axles. Each axle shaft is backed by Alloy USA's exclusive ten-year warranty Our front axle shaft kits include everything you need to complete the job: both passenger and drive side inner and outer axle shafts, U-joints and clips.
- 12173
- 12173
- 804314181734
- Alloy USA
- New
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- Item Requires Shipping
- 34.0 lbs.
- W7.0000" x H7.0000" x L46.0000"
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By Dan Eggen
Washington Post Staff Writer
Wednesday, January 30, 2008
Attorney General Michael B. Mukasey said yesterday that the interrogation tactic known as waterboarding appears to be clearly prohibited by current law in some circumstances, but said that other circumstances would present "a far closer question."
In a three-page letter to Senate Judiciary Chairman Patrick J. Leahy (D-Vt.), however, Mukasey stopped short of offering a clearer legal opinion, because he said the CIA no longer uses the practice on prisoners.
The lawyerly response to demands from Congress was delivered in a last-minute letter before Mukasey's scheduled appearance today before the Judiciary panel. It is likely to anger Democrats who nearly derailed Mukasey's appointment over his refusal to render a firm legal judgment on waterboarding.
Mukasey said in the letter that waterboarding -- a simulated drowning meant to coerce disclosures by a resisting prisoner -- is not part of a "limited set of methods" being used by CIA interrogators. Mukasey said he has found the current methods, which he did not specify, to be legal.
"I understand that you and some other members of the (Judiciary) Committee may feel that I should go further in my review, and answer questions concerning the legality of waterboarding under current law," Mukasey wrote to Leahy. "I understand the strong interest in this question, but I do not think it would be responsible for me, as attorney general, to provide an answer."
Mukasey also wrote that "it is not an easy question" to determine whether the technique is lawful. "There are some circumstances where current law would appear clearly to prohibit the use of waterboarding," he wrote. "Other circumstances would present a far closer question."
In a statement last night, Leahy said Mukasey's response "echoes what other administration officials have said about the use of waterboarding" but does not answer "the critical questions we have been asking about its legality."
"Attorney General Mukasey knows that this will not end the matter and expects to be asked serious questions at the hearing tomorrow," Leahy said.
Mukasey wrote to Leahy that he will answer questions about waterboarding today "to the best of my ability, within the limits that I have described." He also said those limits might make his task "more difficult for me personally" but that he must "do what I believe the law requires."
"Despite disagreements we may have on this issue, I hope that the Committee will respect my judgment on this matter," he wrote.
Mukasey did not elaborate on what circumstances would present a close call on waterboarding. He also did not identify which laws the practice might violate, or explain his reference to "current" law.
Waterboarding was used in 2002 and 2003 by the CIA on three suspected terrorists at secret overseas prisons, and lawmakers have specifically sought Mukasey's judgment about whether it was legal then, under the laws that prevailed during those years. At the time, the Justice Department said it was, but current and former military lawyers, human rights experts, and international lawyers have challenged that position.
One opinion issued by the Justice Department's Office of Legal Counsel in May 2005 authorized a combination of painful physical and psychological interrogation tactics, including head slapping, sub-freezing temperatures and waterboarding, said current and former officials familiar with the issue.
A second document issued by the same Justice Department office in the summer of 2005 asserted that the interrogation practices approved for the CIA did not violate pending legislation to prohibit "cruel, inhuman and degrading" treatment, current and former officials have said.
Waterboarding generally involves strapping a prisoner to a board, covering his face or mouth with a cloth, and pouring water over his face to create the sensation of drowning. The practice dates at least to the Spanish Inquisition and has been prosecuted as torture in U.S. military courts since the Spanish-American War. The State Department has condemned its use in other countries.
Officials have said one of those waterboarded was alleged Sept. 11 mastermind Khalid Sheik Mohammed. The practice was halted after lawmakers raised secret concerns about the CIA's interrogation tactics, officials have said. Mukasey said in his letter to Leahy that he is "authorized to disclose publicly" that waterboarding is not now an approved U.S. interrogation technique.
The CIA's shadowy interrogation program, and its use of severe "enhanced interrogation techniques" on prisoners, has proven to be a pivotal issue for Mukasey. Most Senate Democrats voted against his nomination in November over his refusal to comment on the legality of waterboarding, giving him the lowest level of congressional support of any attorney general in half a century.
In December, the CIA disclosed that it had destroyed interrogation videotapes depicting harsh tactics used on two al-Qaeda prisoners in 2002. Mukasey ordered a preliminary inquiry into the destruction and, earlier this month, assigned a special U.S. attorney to conduct a full criminal investigation. But Mukasey also has rebuffed demands from some Democrats that the investigation be handled by a special prosecutor, and has urged Congress to hold back on its own inquiry while Justice Department prosecutors determine if a crime was committed.
In prepared remarks released Monday, before yesterday's letter on waterboarding, Mukasey said he has sought "to live up to the commitment I made to look for opportunities to work with the Congress" while cautioning that "we will not always agree."
"There are policy initiatives that the department supports that some members of this committee vigorously oppose, and vice versa," Mukasey said. "There also are situations where the interests of the executive branch and the legislature will be in some tension."
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Air LeaseOwners of Air Lease (NYSE: AL) shares as of market close today will be eligible for a dividend of 3 cents per share. At a price of $29.14 as of 9:35 a.m. ET, the dividend yield is 0.3%. The average volume for Air Lease has been 448,300 shares per day over the past 30 days. Air Lease has a market cap of $2.9 billion and is part of the diversified services industry. Shares are up 36.6% year to date as of the close of trading on Friday. EXCLUSIVE OFFER: Jim Cramer's Protégé, Dave Peltier, only buys dividend stocks that have the potential for a 3% to 4% yield and 10% growth. Get his best picks for less than $50/year. Air Lease Corporation engages in the purchase and leasing of commercial aircraft to airlines worldwide. The company also provides fleet management and remarketing services. The company has a P/E ratio of 22.61. Currently there are 8 analysts that rate Air Lease a buy, no analysts rate it a sell, and 3 rate it a hold. TheStreet Ratings rates Air Lease as a sell. Among the areas we feel are negative, one of the most important has been very high debt management risk by most measures. You can view the full Air Lease Ratings Report now.
- See our top-yielding stocks list.


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Clare Short, the former development secretary, gave richly entertaining evidence to the Iraq inquiry. It was like watching a highly respected international statesperson whose brain was being taken over by Little Britain's Vicky Pollard. Answers tumbled out at endless length.
She would give her overview of the Middle East situation, and then suddenly you could almost hear her saying: "Yes but, no but, that Tracey Blair, she's a liar she is, and that Sharon Bush, she's no better, they was fibbin', they hate me, yes but …"
The inquiry seemed distressed by her tone. This is not the way international statespersons are supposed to talk. She painted a picture of the world today if her advice had been heeded: a Palestinian state, peace in the Middle East, and a "beautiful" UN resolution! Everything would have been better.
"A counterfactual universe," said Sir John Chilcot, in a voice so dry you could have used it to set a permanent wave. The public loves politicians who say what they mean; mandarins can't stand them.
She had wonderful portraits of the way we are governed now. Cabinet meetings were meaningless. "We had little chats," she said three times, as if they were water-cooler conversations about, say, last night's TV. All decisions were taken elsewhere. In cabinet, "Tony would say: 'Jack, you've been to see Colin Powell' – which had been in the press already – 'tell us how the meeting went,' and Jack would make little jokes, as he does."
At the meeting just before the invasion, when the attorney general suddenly revealed that he had completely changed his mind on its legality, she had said: "It's late. Why is it so late?" Then: "Everyone went [here she made a noise which I recorded in my notes as FWOUGHHH!]. I was jeered at to be quiet."
I loved the idea of the cabinet jeering, like Millwall fans at a West Ham match. "She's all gone noisy over there!"
Everyone who should have been involved was, she thought, told nothing. Whereas Alastair Campbell claimed Gordon Brown had been at the centre of things, according to Clare he had been abandoned at the edge. "Gordon was pushed out with me, and marginalised, and he would have cups of coffee with me, saying Tony Blair is obsessed with his legacy and he thinks he can have a quick war and then a reshuffle."
She seemed fixated on the intake of caffeine. "I had various cups of coffee with Gordon. He was very unhappy and he would talk about other issues than Iraq, and I would rabbit on about Iraq, I'm not sure we were communicating terribly well, but we were having cups of coffee." By the end they must have been so wired and so angry that the stuff spilled all over them.
She had been conned into not resigning immediately. Tracey Blair had told her that Sharon Bush had promised to follow the Middle East road map, and that the UN would lead the reconstruction of Iraq. But had she taken the right decision at the time?
"I still agree with myself" – a line only a politician could produce. As she left the room, the onlookers burst into applause. Unlike the inquiry, they adore anyone who sticks it to Tracey.


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Glock's 30S subcompact pistol is a .45-caliber hybrid of the company's Glock 30 and Glock 36 that offers a larger frame to accommodate 10-round capacity in a slim package. Read A.J. George's full "Arsenal" review of the firearm. Photos courtesy of Mark W. Clark.
Author Lynne Finch joins us to discuss weapon handling, off-duty carry, and choosing a concealment holster for women in law enforcement. Her book, "Taking Your First Shot: A Woman's Introduction to Defensive Shooting and Personal Safety" was published in June. She also discusses situational awareness.


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Buying Credits
I bought credits from Amazon for 19.99. My game froze and I didn't get my credits. The Bingo Blitz people have been giving me the run around. I have sent them a copy of my purchase and filled out the complaint ticket twice. They still keep sending me the same " unfortunately" carp, if I don't get the credits I bought I will be reposting this every day, so you won't get the treatment I have.
4 Replies
That's horrible Don't stop until you get the credits or at least a refund.
Thank you bingo blit
Thank yu
That happened to me with Amazon and I have still not got a refund. This was in December 2015, and I was using a prepaid card!


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Matt Cohen: The Rodeo Trail
by Matt Cohen
About the Book
Matt Cohen – The Rodeo Trail:
An 80-page rodeo picture book featuring pictures from 2007-2012 by award-winning photographer Matt Cohen. This book includes a variety of pictures from quiet moments behind the chutes to peak action, black & white to vibrant color, portraits to buck-offs. You simply will not find better rodeo photography anywhere.
Pictures in the book were made at the Red Bluff Round-Up, Reno Rodeo, Clovis Rodeo, Calgary Stampede, Mother Lode Round-Up, Livermore Rodeo, Oakdale Rodeo, Marysville Stampede, National Finals Rodeo, Grand National Rodeo, Best Buck in the Bay, as well as practice sessions at the Growney Brothers Ranch and B-Bar Arena.
Features & Details
- Category Sports & Adventure
Standard Landscape, 10×8 in, 25×20 cm
80 Pages
- Publish Date Apr 10, 2012
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At the heart of all natural disaster preparedness is considering whether you have enough food to see you through the disaster. Many people don't. There are far too many people out there who believe that their government will help them in their time of need.
A change of perception is needed here, as it is unlikely that the government will always be able to help them out! This may be due to the authorities not having the resources or not being able to reach those in need quickly enough.
Source: survivalguidehub.com


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Overview Edit
Prior to 1976, national security investigations at the FBI followed no specific guidelines established by either the Department of Justice or Congress. Without oversight, the agency developed a covert Counterintelligence Program (COINTELPRO) to target the Communist Party U.S.A. During its lifespan from 1956 to 1971, the program involved aggressive and illegal tactics to harass, disrupt, discredit, and collect intelligence on the party and its members. COINTELPRO's purpose was to protect national security, prevent violence, and maintain the social and political order in the United States. It was not designed to build traditional cases to be brought to trial. The FBI expanded COINTELPRO to target groups and movements such as the Socialist Workers Party, the Ku Klux Klan, the New Left, and the Black Panther Party. The program was developed partly because the FBI was frustrated with the U.S. Supreme Court limits on overt investigations of dissident groups.
With COINTELPRO, the FBI "took the law into its own hands" and authorized questionable methods including "use of subterfuge, plant[ing] agents provocateurs, [and] leak[ing] derogatory information to the press." Among specific tactics, the FBI mailed anonymous letters to break up marriages, contacted employers to get people fired from their jobs, and falsely declaimed individuals as government informants to discredit them within their own organizations. The agency even targeted some nonviolent organizations, such as the Southern Christian Leadership Conference, because it "believed they represented a 'potential for violence.'" As the FBI itself acknowledges, some COINTELPRO methods were excessive and "went too far for the American people."
The public first learned of the program after a 1971 burglary at an FBI office in Media, PA. Individuals tied to the incident leaked information on COINTELPRO to the press and Congress. In response, the FBI terminated the program.


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Piñatarama / Museum of Modern Art / México City
Piñatarama / Museum of Modern Art / México City
Piñatas are just becoming popular around here but they are as symbolic as coffee or salsa to my culture. I grew up with crazy piñata parties begging to be blindfolded and span around, armed with a broom stick and then released in search for the treasure amidst a loud group of hungry-for-candy monsters. I still remember the rush of throwing blind ninja moves with the stick, trying to remain standing, drunk of laughter, hearing the bang! followed by kids screaming and then diving down with the crowd to fetch a few candies, whistles and plastic soldiers
I felt something quite similar when fellow illustrator Cecilia Meade asked me to participate in Piñatarama, a marvelous color fiesta featuring 40 Piñatas designed by artists from around the world, masterfully crafted by Mexican artisans. The show opened on June 11 at the Museum of Modern Art in México city and will be on view until September
Many thanks to Dr. Alderete, Clarisa Moura, David Gamez and of course to Cecilia for coming up with such a beautiful idea and for their kind invitation. My respect and admiration goes out to the piñateros, Ricardo Linares, Lorenzo García, Marcial García and also to the Museum for supporting this kind of exhibitions
photo: lugh
For more information about the project and a complete list of the artists, please visit pinatarama.com ^ above pic: lugh
Preliminary sketch of my flying burrito and the finished piñata
Rotations delivered to the piñateros as guidelines for my design, and below, a sweet video tour of the event
Piñatarama MAM from VértigoGalería on Vimeo.
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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
We believe that if one user has a question, there could be more users who may have the same question. This is why many of our support forum threads are public and available to be searched and viewed. If you'd like help immediately, feel free to search for a similar question, or submit your question or concern.
Forms Central UpgradeAsked by inlinesolutions on May 26, 2015 at 04:30 PM
I'm looking to upgrade to your system this weekend.
I want to migrate all of the information at one time.
Concern at this point is to ensure all of my users who will be former Co-Authors will need their accounts setup. Please provide me with information on instructions I should provide them to setup their accounts with your service before I move everything over from Forms Central to Jot Form.
JotForm Support
Hi Scott,
You can migrate your forms and data from Formcentral to JotForm by following this user guide:
Concern at this point is to ensure all of my users who will be former Co-Authors will need their accounts setup.
I am not really sure how you meant this, by if my understanding is correct, you wanted to have JotForm account for "Co-Authors" also?
If it is, they can sign-up for an account also just using at http://www.jotform.com/signup
Hope this help. Please let us know if there is more we can assist you with.


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Hero launched the new Duet scooter at Rs 48,400 (ex-showroom Bangalore). The Duet employs full metal body and has a claimed mileage figure of 63.8kmpl and will rival the likes of the Honda Activa 3G, TVS Jupiter, Yamaha Ray and Suzuki Lets
Duet is based on a new platform and comes with full metal body. The Hero Duet will be the second scooter to employ a non-Honda engine and will be powered by a indigenously developed 110-cc air-cooled, 4-stroke single cylinder OHC engine, producing a maximum power output of 8.5PS at 8,000 rpm and maximum torque of 8.3 Nm torque at 6,500 rpm. Hero claims that the Duet scooter has an overall mileage of 63.8kmpl which is among the highest for scooters on sale in India. The Hero Duet has been priced at Rs. 48,400 for the LX version and Rs. 49,900 for the VX version (both pricing are ex-showroom Bangalore).
Hero MotoCorp Ltd expanded its scooter portfolio and launched the new Duet scooter in India. Hero had showcased the new Duet earlier this month and hinted at a festive season launch.


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Flavio E. Goncalves was born in 1966 in Brazil. Having a strong interest in computers, he got his first personal computer in 1983, and since then, it has been almost an addiction. He received his degree in engineering in 1989 with a focus on computer-aided designing and manufacturing.
He is also the CTO of SipPulse Routing and Billing Solutions in Brazil—a company dedicated to the implementing telephone companies based on SIP, VoIP providers, and large-scale new generation telephony systems. Since 1993, he has participated in a series of certification programs and been certificated as Novell MCNE/MCNI, Microsoft MCSE/MCT, Cisco CCSP/CCNP/CCDP, Asterisk dCAP, and some others.
He started writing about open source software because he thinks that the way certification programs have worked is very good for learners. Some books are written by strictly technical people who sometimes do not have a clear idea on how people learn. He tried to use his 15 years of experience as an instructor to help people learn about the open source telephony software. Together with Bogdan, he created the OpenSIPS boot camp followed by the e-learning program, OpenSIPS eBootcamp.
His experience with networks, protocol analyzers, and IP telephony combined with his teaching experience gave him an edge to write technical books. The first one was Configuration Guide for Asterisk PBX, by BookSurge Publishing, the second was Building Telephony Systems with OpenSER, by Packt Publishing, and the third was Building Telepopny Systems With OpenSIPS 1.6, by Packt Publishing.
As the CTO of SipPulse, Flavio balances his time between family, work, and fun. He is the father of two children and lives in Florianopolis, Brazil—one of the most beautiful places in the world. He dedicates his free time to water sports such as surfing and sailing.