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"arthritis problem with GP"
About: Greater Manchester Greater Manchester
Posted by in need of help from the nhs (as ),
Around three years ago started with severe pains in my hands stiffness and dropping things and weakness in my hands and I felt generally unwell tired my GP sent me for a scan on my hands but they could not find anything at the time I had another health problem a fistula on my breast that was constantly infected and the GP said that this was related to the problem in my hands. The consultant dealing with the fistula disagreed. the problem eased with time in my hands but returned last December but it was as bad in my feet and hands and some times my hips I returned to the GP and was signed off work blood tests revealed nothing untoward although I have a vitamin D deficiency I was treated for this and given pain killers and anti depressants as I was very emotional due to the unexplained pain and feeling very tired. This problem has continued since then I have been off work and at my wits end due to the life changing pains in my hands and feet the pain killers do not really help the anti depressants help me sleep the doctor said it is the depression thats causing the pain. I applied to see a doctor on a television programme who did ultra sound scans on my hands and found evidence of seronagative arthritis and carpal tunnel syndrome in my hands a have canker sores in my mouth and cannot walk or properly or lift anything at the moment with out causing pain even with this knowledge I have to wait two weeks before I can see my GP I feel like a mithering person and other than increasing my anti depressants the GP has done nothing for me I told them I was down becasue of the pain and they told me the depression was causin the pain, I am close to losing my job and do feel with a referral to a specialist I would have avoided a lot of pain and heartache very disappointed with my GP.


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5973 SW 128th Avenue, Fort Lauderdale, FORTLAUD, 33330
$695,000 49,658 sqft
Within the superb equestrian Town of Southwest Ranches is the understated luxury subdivision of Sunshine Ranches. StoneCreek at Sunshine Ranches is a 7-homesite enclave where each residence boast privacy gates and a minimum of 2+ acres of land. Lot 1 is one of two lots that remain in the rapidly expanding development which is currently home to two massive custom estates, and in production to build two more. This is an incredible opportunity to build your dream estate. Please schedule a site visit today!
Wrong Location? FIND ME

subset: commoncrawl


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ZEUS (Fractal R4 | Blue/Black | Internal UT60 Rad) - by alpenwasser [COMPLETE]
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Featuring Model Prices and Specifications, plus Reviews,News and Videos.
THE LIFETIME motorsport ban handed down to Flavio Briatore by the FIA in September last year has been overturned by a French court which has ...
Interested in a new car? Simply tell us the vehicle you are interested in and we can take care of the rest.
Get great offers, the latest news and reviews, photos, videos and more delivered straight to your inbox.


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- Latest
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The Complete Guide to Twitter Measurement Download Gibson is one of the biggest and most respected brands in the music industry, best known for its legendary Les Paul guitar. This classic instrument is played by rock stars and the fans filling stadiums to see them, too. Being an avid music lover and rock 'n' roll enthusiast in addition to a Market Development Representative ...


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Butterick 4395; ca. early to mid 1980s. "Fast & Easy" Misses' Dress, Petticoat & Sash. Loose-fitting flared dress three inches below mid-knee has round neckline, elasticized waistline, extended shoulders and sash. Flared petticoat, mid-calf length has elasticiized waistline and hemline ruffle. Topstitching.
Links to reviews/blog postsEdit
- Sew Exciting Needleworks
- MOMSPatterns
- Pretty Pattern Shop
- Shelleyville Size 6-8-10, Bust 30 1/2 - 32 1/2
Add a photo to the gallery by clicking the "modify" button below.
[insert your username, and make sure your preferences allow for people to contact you via e-mail. Do not list your email address here!]


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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
At JotForm, we want to make sure that you're getting the online form builder help that you need. Our friendly customer support team is available 24/7.
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Can I create Forms in following formats: Web, PDF, and Embed?Asked by AmericanSystems on April 13, 2015 at 01:56 PM
Also, does JotForm provide an email receipt of submitted material to the respondents as well as the host/surveyor?
We want the person submitting the information to see what was submitted as a receipt. As the administrator, we want to make sure we receive an email with the data submitted as well.
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Blog Entry# 2040007
Posted: Oct 28 2016 (13:22)
No Responses Yet
Journey: Wed Nov 02, 2016 @ 06:3522105/Indrayani SF Express
Class: Chair Car - CC | Tot Seats: Avbl 97
Travel: KYN/Kalyan Junction to PUNE/Pune Junction | 138 km / 190 km
Entry# 2040007
PNR: 853-xxxxxxx | Similar PNRs
Posted Status as of: Oct 28 2016 (13:22)Passenger 1: Waitlist# 18 (Booking Status: W/L 25,GNWL)
Total Fare: 305
Chart NOT Prepared
Please analyze this PNR, and predict the final Charting Status. Thanks.
Latest Status as of: Nov 01 2016 (21:24)Passenger 1: C2,58 [AS] (Booking Status: W/L 25,GNWL)
Total Fare: 305
Chart PREPARED
ARP (Advanced Reservation Period) Calculator
Reservations Open Today @ 8am for:
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Submitted by: Stacy Saunders
Prep Time: 3 Minutes
Cook Time: 7 Minutes
Servings: 2
This is my "clean" version of my french toast recipe. I haven't figured out the breakdown of fat/proteins/carbs
mix the coconut sugar, cinnamon and vanilla and then beat the egg and milk mixture together. Soak bread slices to your desired level of moisture -- more well soaked makes it almost like bread pudding when cooked. Spread 1 T of coconut oil in a pan and cook the french toast for a total of 3 minutes on each side - flipping after 1 minute. Garnish with organic strawberries, blueberries and blackberries and serve with pasture butter and organic maple syrup (if you have a serious sweet tooth). This is a sweet french toast so you can eat it just plain as well.


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FEBRUARY 19-21 ITSC 2012, Portland, Oregon. Instructional Technology Strategies Conference is an active, hands-on technology and strategy learning conference presented by the Organization for Educational Technology and Curriculum (OETC), an organization comprised of more than 800 educational institutions, school districts, colleges, and other non-profit educational organizations throughout the U.S. Learn all about the conference, featured speakers, the "unconference"—and their TED-like Soiree of Slides. Good stuff! Check it all out right here.
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Acme Markets to Close Two Stores
Underperforming locations 'not meeting goals'By Bridget Goldschmidt, EnsembleIQ
Two underperforming Philadelphia-area Acme supermarkets, located in Chalfont and Warminster, Pa., are slated to close next month.
Angela Perez, Acme's manager, communications and public affairs, told PG that associates at the stores were informed "that their locations would be closing as a result of not meeting company goals." Added Perez: "The stores will close by Nov. 13, 2014, with liquidation sales beginning on Nov. 24. Associates who are interested in remaining with the company will have the opportunity to transfer to open positions at nearby stores."
The decision to close the stores was made because "Acme is committed to running as many profitable stores as possible," she explained. "Like any responsible business owner, we evaluate our locations frequently and do everything we can to increase profitability and gain customer traffic so that we can further invest in our store base."
Despite the planned closures, Perez noted that Acme was still expanding. "We are in the process of building a new store in Beach Haven, N.J.," she said. "In addition, we are in the middle of a significant remodel in our Chestertown, Md., location. Finally, we've added beer sales, with the opening of the Frosted Mug [beer cafés] in our Flourtown, Pa., [store] and 19th and Oregon location in Philadelphia. Future store remodels and Frosted Mug openings are currently underway."
About 130 associates are affected by the store closings, local TV station NBC10 reported.
Owned by Boise, Idaho-based Albertsons LLC, the grocery chain currently operates 110 stores in Pennsylvania, New Jersey, Delaware and Maryland.


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It's often rock music that's generally classified as good driving music, which it is no doubt, but maybe we could add in some more stuff as well. We've come up with a list of songs that our generation should know and have enjoyed in the past—a least a majority of it—but may have forgotten about, that makes for good driving tunes.
We understand it's not a very conventional list, but believe your driving music should keep you cheery, so give a shot in your player. Here's to many miles of happy motoring with the the windows down and the radio up, like a very popular musician in this list talks about.
1. Jazzy Jeff and The Fresh Prince - Summertime (Year of release: 1991)
Summer is well and truly on its way, and what better track than Summertime to set the atmosphere right in your car. Hip hop doesn't get much smoother than this, and the combination of Jazzy Jeff's ultra-chilled beats and the French Prince's (a young Will Smith) easy flow will always make an impressive entrance to Sunday brunch at your mate's in your pride ride.
2. Tom Petty - Learning To Fly (Year of release: 1991)
Rock needs to be represented, obviously, and the genius that is Tom Petty is known to most as the singer of the ubiquitous Free Fallin', but this song is easily one of his best. Add it to your car playlist because few tracks are better suited to the road, with its brilliant score and lyrics that will drive away any work blues that insisted on tagging along for the trip.
3. The Grid - Swamp Thing (Year of release: 1994)
You would think a banjo and techno is one of the least likely combinations that will work, but the iconic Swamp Thing will accompany 100 km/h like few tracks can. Just make sure the amazing beat doesn't over-excite you though, we certainly don't want to profess unsafe driving habits. Because this was easily one the best songs of the early 90s, and complements energetic driving very nicely.
4. Lighthouse Family - Free (Year of release: 2001)
You've had a rough couple of months at the cubicle, and have finally got the time to leave for a well-deserved break. The traffic is thinning out, and you're on the highway at last, on your way to Goa. That's when you should chuck this track on, because you're guaranteed to forget all your troubles, and begin soaking in the fact that you're finally off...
5. Planet Funk - Chase The Sun (Year of release: 2001)
Tempo change now. Another great driving single that's lying forgotten in a CD grave somewhere, is Planet Funk's Chase the Sun, which works especially well if you're on your way to catching up with your buddies from school in town. It's obviously not a track with the deepest lyrics or anything, but who said catchy tunes can't be good driving music?
6. Milky - Just The Way You Are (Year of release: 2002)
Don't laugh, give it a shot, because music doesn't get you much happier than this song can, and what better time to get cheery than during your road trip. Just The Way You Are has one of the most infectious hooks of our generation's tunes, but it's a song that's lying forgotten in your 1990s collection. So plug in your thumb drive into your stereo, and get into a good mood.
7. Jakatta Feat. Seal - My Vision (Year of release: 2002)
My Vision samples a very famous track by Thomas Newman–Brooks Was Here–which played a part in the Shawshank Redemption movie soundtrack. But get on to a long, straight stretch of expressway, and this is the song to play. You won't feel like stopping for anything if this track is playing, so make sure all loo, smoke, and chow stops have been finished in advance.
8. Nelly - Pimp Juice (Year of release: 2003)
Every car needs at least one track to get the subwoofer booming its best, and if reproduced well, the bass line on Pimp Juice will vibrate some sense into those spoiled kids in your block who think that Pitbull is the epitome of stereo-show-off music. If base is the case, it's all about Nelly...
9. Bryan Adams - Open Road (Year of release: 2004)
From the Room Service album, Bryan Adam's Open Road may not be his most well known song, but it's the perfect one to put you in highway spirits. A simple, pure song about freedom and love for highway escapades, Open Road captures the joy of motoring with uplifting rock music. So don't make fun of Bryan Adams, because he gets the wheelin' world...
10. Avicci - Levels (Year of release: 2011)
We'll end this list with something even the aforementioned Pitbull fan club will recognise. But save this one for when you know it's an open stretch of road with minimal traffic. While we don't encourage rash driving, we know that some songs keep you fixed in the driving zone. This is definitely one of 'em, and we guarantee you that this remix of the Etta James classic is great accompaniment for your gear changing.


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Antoine Moueix 2007, Ac St Emilion Grand Cru
Bordeaux Right Bank
Bordeaux, France
$27.95 (Agent)
88 pts
Critic Reviews (1)
Julian Hitner
Dark-opaque ruby in colour, the 2007 Antoine Moueix is lightly toasted, revealing fairly developed notes of red...Sign In to see Critic Reviews. Not a Member? Please Sign Up.


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Cornwall 33
TRIES: Notman (2), Goodfellow, Williams, Vinnicombe. CONS: Lewitt (4).
Gloucestershire 27
TRIES: Bozzard, Fielding, Molitika. CONS: Fielding (3).
PENS: Fielding (2).
Gloucestershire provided Cornwall with a stern test last Saturday at Camborne's Recreation Ground and, despite some late controversy, the result was a deserved win for the home side.
The win means that Cornwall's match with Hertfordshire on Saturday becomes a winner-takes-all decider for who will play at Twickenham in the Bill Beaumont Cup final, with both sides tied on ten points.
Cornwall's match didn't get off to a good start as two penalties were conceded in the first 15 minutes, which were both converted by Leo Fielding. The away side showed real intent and Cornwall did well to limit the scoring to six points.
The attacking prowess of Cornwall shone through soon after though, as Tom Notman touched down after an excellent long pass from Ryan Westren. Neat passing from Greg Goodfellow and Chris Fuca saw the former score Cornwall's second try in four minutes soon after.
Just before the break however, ill discipline saw Jamie Salter and Joe Smart sent to the sin bin. Gloucestershire took advantage of their numbers to score a try and go in to the break one point ahead at 13-12.
In the second half it was Cornwall's turn to start quickly as Craig Williams charged forward to score a try after just two minutes.
Gloucestershire again proved tenacious though, and struck back soon after with Fielding scoring after some good passing from the visitors.
The see-saw swung back Cornwall's way though as they scored two tries. Lewis Vinnicombe charged down a kick to score the first, and Notman scored his second after a good drive from the forwards.
There was late drama though as Gloucestershire set up a tense finale, the referee awarding a try despite Loti Molitika appearing to ground the ball short of the line for the visitors.
Cornwall were to hold on, however, and now look forward to their match with Hertfordshire when they will vie for a place at Twickenham.
Cornwall coach Ian Morgan said: "The match against Gloucestershire was never going to be easy with people's expectations high after last week's win over North Midlands.
"Everyone involved with the squad knew this was going to be a tough mountain to climb; Gloucestershire teams are renowned for their power and were out to avenge their defeat of last week against Hertfordshire. The sun was lovely for the spectators but was really telling on the players and so we used our rolling substitutions and lots of hydration during the game.
"Everyone involved played with heart and pride to ensure a climax to the pool stage next week at Hertford RFC".


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Conditional required fields not updating when original selection changedAsked by Sian on February 10, 2016 at 05:29 AM
We have set up conditional fields (required) which respond to a drop-down selection.
For example: If select option a, conditional options appear as normal. However if I change my mind and then select option b instead of a, the conditional options for a. do not disappear and I am then stuck - as the options no longer correspond to the question.
What is the fix for this?
JotForm Support
Since you are using a form collapse, you should set it to hidden and open. Here's our complete guide on how you can show/hide fields by form collapse:
When using this method, I would suggest that all fields under one option should be added to a single form collapse. One form collapse for Writer and one form collapse for the director and so on.


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Video Editor
Experienced video editor with three years experience in National News, proficient in Adobe Premier Pro with experience in Photoshop and aftereffects.
Training & Qualifications
3 years experience in national news shooting, editing, producing, managing.
Masters in Business Administration
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I Strive to attain maximum efficiency for myself and my co-workers. I have worked from the ground up with NewsmaxTV, a National News Broadcast Company, from it's infancy on the internet. I created t...he standard operation procedure for post production, asset management, and third party content. In less than two years I worked up from Scheduler, to Production Assistant, to Editor/Assistant Producer, to Lead News Editor, to Video Production Manager. I organized the editorial staff to create a universal standard operating procedure and I tripled the output of the most valuable assets while working with half the original staff. Education includes a Masters in Business Administration with a Bachelors in Philosophy and a minor in Political Science. I have three years broadcast experience not including freelance work. I have superlative communication and interpersonal skills and work well in a team environment. show moreOpen to other proposals
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From Marsell, these deer skin ankle boots feature an almond toe, exposed, tonal back zip closure, and a chunky low heel. A suede upper with a distressed finish creates a rustic, vintage interpretation of a classic boot. Details include a heel cap and tonal pull tab on the zipper.
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Our dear friend Russell B. Johnson answers a very important question from a viewer about how superheroes.
Superheroes can be real jerks, can't they?
This is an archived article and the information in the article may be outdated. Please look at the time stamp on the story to see when it was last updated.


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... a Spatial Solutions Company
RouteMan – The Route Management System from KCS provides an efficient, flexible and accurate way for companies to manage route delivery and route sales. RouteMan is easy to learn and simple to operate. An integral part of RouteMan is RouteMobile, the smart device handheld module of RouteMan. Orders are entered into RouteMobile at the customer's site and a professional, printed invoice is available. Data is automatically uploaded from RouteMobile to back office PC via the internet. An optional module is available to keep up with Equipment Inventory and Maintenance. RouteMan is currently used by such industries as Packaged Ice, Bottled Water, Propane Gas, Coffee Service, Beauty Shop Supplies, and Pre-paid Calling Cards.


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Hold it down for the Motor City in the New Era 950 'Detroit Tigers' Trucker (70324489). This bold black hat features a white mesh trucker panel and Detroit Tigers logo embroidered on the front of crown. A black New Era flag branding is embroidered on wearer's left side and a second Detroit Tigers logo is found above the adjustable sizing enclosure at back of hat. Pair this hat to look fresh with any outfit. For sizing information, be sure to check our Size Chart. Looking for a store near you? Head over to our Store Locator.
© CityGear.com 2017


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MATLAB Newsgroup
Well, what i'm trying at is creating a *.m file which will define a function and then i wanna INTEGRATE the function from the command window by a call with suitable arguments using 'quadl' command(say). The function goes like-
function [F]=strain_time_newt(max_str)
Command Window :
However, the integration couldn't be carried out and an attempt in doing so gives the message
??? Error using ==> rdivide
Matrix dimensions must agree.
Error in ==> strain_time_newt at 13
Error in ==> quadl at 64
y = feval(f,x,varargin{:}); y = y(:).';
how am i going to get over this problem? please suggest.
Subrata Chandra <email@example.com> wrote in message <email@example.com>...
> Well, what i'm trying at is creating a *.m file which will define a function and then i wanna INTEGRATE the function from the command window by a call with suitable arguments using 'quadl' command(say). The function goes like-
> function [F]=strain_time_newt(max_str)
> estep=0.01;
> s=0.01:estep:max_str;
> P=s./max_str;
> F=(1-P)./(1-E.*s.*(1-P));
> end
> Command Window :
> >>strain_time_newt(2.0);
> >>quadl(@strain_time_newt,0,10);
> However, the integration couldn't be carried out and an attempt in doing so gives the message
> ??? Error using ==> rdivide
> Matrix dimensions must agree.
> Error in ==> strain_time_newt at 13
> P=s./max_str;
> Error in ==> quadl at 64
> y = feval(f,x,varargin{:}); y = y(:).';
> how am i going to get over this problem? please suggest.
- - - - - - - -
Your m-file is not properly prepared. The function that is passed to it must be able to accept a vector of integrand values that 'quadl' chooses and to do so many times during a single integration process, not the scalar you have apparently assumed here. It looks as though you are trying to decide for 'quadl' what integration variable values it should use. That is not how 'quadl' works. It is not clear what function your integrand is, but whatever it is, you should write your function so that it can produce vector-valued integrand values corresponding to the vector of integration variable values passed to it. Read the documentation!
Roger Stafford
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text:
Angel 3D card
The cover of this ornate pop up Christmas card shows an illustration of a detailed laser-cut angel floating magically.
Open the shimmering, golden cover to reveal the elegant angel sculpture contained within. The Christmas angel has her hands clasped solemnly, and her intricate wings expanded beautifully.
Inspiration for the Angel Pop-Up Christmas Card:
The angel card is inspired by the classic Christmas adornment. Who doesn't remember feeling like a bird while being lifted high off the ground to put the finishing touch on the tree?
Occasions for the 3D Pop-Up Angel Christmas Card:
Use this pop up card throughout the holiday season. Lovepop Christmas cards are a gift in and of themselves. Give the angel card to friends and family and delight them with its beauty.
This beautiful angel card is 5 inches wide by 7 inches tall.
One Lovepop pop-up card with one blank envelope.
Related collections:
Christmas Cards       Holiday and Christmas cards       Holiday and New Years       Holiday cards with a personal touch       Sympathy Cards       Use Your Imagination


text:
Question: On May 18 Stanton Electronics purchased on credit 1 000 TV
On May 18 Stanton Electronics purchased, on credit, 1,000 TV sets for $400 each. Stanton plans to resell these TV's in its store. Stanton paid the supplier on June 30.
Prepare the necessary journal entry (or entries) on May 18 and June 30.
View Solution:
Prepare the necessary journal entry (or entries) on May 18 and June 30.
View Solution:
Answer to relevant QuestionsOn February 15, Barbour Industries buys $800,000 of inventory on credit. On March 31, Barbour approaches its supplier because it cannot pay the $800,000. The supplier agrees to roll the amount into a note due on September 30 ...GCD Advisors offices are open Monday through Friday and GCD pays employees salaries of $50,000 every other Friday. During the current year December 31 falls on a Thursday and the next payday is January 8. Required: Given ...Weston Cellular provides wireless phone service. During April 2012, it billed a customer a total of $135,000 before taxes. Weston also must pay the following taxes on these charges: a. State of Illinois sales tax of 7 ...Kramerica, Inc., sold 350 oil drums to Thompson Manufacturing for $75 each. In addition to the $75 sale price per drum, there is a $1 per drum federal excise tax and a 7 percent state sales tax. What journal entry should be ...Daniels Company engaged in the following transactions during 2012: a. Purchased $25,000 of merchandise from XYZ Supplies on January 26. Amount due in full on February 28. b. Paid for 40 percent of the purchased merchandise ...
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2015 Was a Rough Year for Kids in Arizona's Child Welfare System
Andrew J. Nilsen
2015 has not been a good year for Arizona's most-vulnerable population: kids in the child welfare system.
In early February, without much explanation, newly appointed Governor Doug Ducey fired Charles Flanagan, director of Arizona's Department of Child Safety, and in his place, installed Greg McKay, a police officer who headed the Office of Child Welfare Investigations —the law enforcement arm of the DCS.
It's not unusual for a new governor to make leadership changes at the top of agencies, but McKay's appointment was controversial. He was seen widely as unqualified for the position and many worried that his law enforcement background would be a bad fit for a child welfare agency.
Ducey and McKay shrugged off any criticism, and promised that this leadership change was exactly what the department needed.
At the time, the DCS was still a new agency, having only been created a few months earlier because the previous child welfare agency, Child Protective Services, essentially imploded in the wake of a scandal about thousands of un-investigated reports of child abuse and neglect.
After the so-called "not investigated" or "NI" scandal came to light, former governor Jan Brewer dissolved CPS in January 2014, and with the state Legislature's approval, created the DCS and put Flanagan in charge.
As New Times wrote in a cover story earlier this year, Flanagan was making positive strides. Under his leadership, the DCS tackled the un-investigated cases, fixed problems with its child abuse hotline, increased staffing, and began the process of replacing an outdated computer system.
Employees who spoke with New Times described Flanagan as approachable, and consistently said that for the first time in years, morale at the agency was on the upswing.
Hence, it was a big surprise when Ducey replaced Flanagan.
One of McKay's first goals was to fix the backlog of "inactive" cases — open cases that haven't been touched in the computer system for 60 days — which had grown to include more than 13,000, and which he had said Flanagan did a poor job handling.
But during the first few months of McKay's tenure, he fired or demoted many employees — including some who had been at the agency for decades – and dozens of others quit or requested to be transferred to a different state agency.
McKay described the turnover as typical institutional growing pains, though some speculate it had a lot more to do with the so-called "culture of fear" many say he created at the agency.
Greg McKay, DCS Director
Arizona Department of Child Safety
At legislative Child Safety Oversight Committee meetings, McKay assured the room that things were getting better: he said he had plans to hire and train new staff, to fix the agency's antiquated computer system and to create a better foster care experience for children and families.
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The committee repeatedly expressed frustration with the lack of data and hard statistics by which improvement could be measured – an issue not helped by the fact that under McKay, the DCS has submitted regular progress reports months after they were due.
The Editorial Board of the Arizona Republic took the bold step recently of calling for McKay's resignation, though a handful of state legislators shot back and said McKay deserves "a fair chance" and more time.
(To be fair, the DCS was far from flawless when McKay took over, and everyone agrees it's unrealistic to expect all of the agency's troubles and challenges to be solved within a year. It's also unclear whether more knee-jerk reactions would help or hinder the DCS.)
-JosephB-/Creative Commons
But, the big question – perhaps the only question – is whether children in Arizona are faring better than they were before.
Given the following list of five things that didn't happen at the DCS during 2015, it's a little hard to say that the kid are all right.
5. The number of backlogged cases didn't go down.
In fact, it increased from about 13,000 to almost 15,000 since McKay took over.
4. Because of the 33 percent staff turnover rate, the DCS didn't meet its employment goals.
Staff turnover rate was about 26 percent at the end of 2014.
3. The DCS' antiquated computer system, CHILDS, wasn't updated or fixed.
According to Michael Dellner, deputy director of operations, the first round of changes won't take effect until 2018.
2. The DCS didn't please or gain the trust of state lawmakers.
The agency got an "unfavorable" review, akin to a failing review, at the most recent Joint Legislative Budget Committee.
1. The number of kids in state care has gone up, not down.
There are 19,000 kids in state care, as opposed to 16,000 last January.
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Model Mayhem Admin
StrandMachineHair Stylist Female Tampa, Florida, US
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My interest in fashion and cosmetology started at 16 years old when introduced to fetish and lolita goth photography. A huge fan of industrial and ebm music I began clubbing at 18 building my wardrobe and creating hair pieces and make up designs for each event I would attend. At 18 I also took my first salon job as a receptionist. I continued to work as a salon manager for years. In 2002, with my popularity growing in the South Florida fetish and goth scene I started StrandMachine hair designs. Designed, made, and shipped out hairpieces to a world wide clientel.
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I graduated from The Aveda Institute in St. Pete FL 6/07 and moved back to Miami. Worked at the Retreat Salon/Spa in Ft. Lauderdale for a year then decided Tampa is my true home.
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50% OFF! - God's Fall Paintbox Clusters
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text: Advancing Adhesives On the Bonding Edge A new reworkable edgebond adhesive enhances board-level reliability of large WLCSP. February 1, 2017 No Comments A new reworkable edgebond adhesive* from Zymet has been shown to enhance the board-level reliability of a large wafer-level chip-scale package (WLCSP). The work was performed in a collaborative effort between Portland State University, Cisco, and Zymet, and published in the Proceedings of the SMTA International 2016 conference, which took place in Rosemont, Ill.Read More


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Problem with Ansys meshing
Hi Dears;
I have a 3.D. geometry and I am obliged to generate a logical mesh for this fairly complex geometry. My exceed version as an essential tool for meshing is Exceed V6.1 and Gambit version is Gambit 2.2.30. But whenever I wanna execute Gambit, Gambit isn't loaded. What's the probable problem?
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Gambit/Exceed issue.
You should maybe try again with a better title so that Gambit/Exceed experts will look at it ;)
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Start the holiday festivities off right with these easy-to-make savory snacks.
30 Homemade Food Gifts for the Holidays
Have some fun with these sweet starter recipes to spread all the holiday joy.More
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Wow your guests with this great-looking appetizer that's also extremely delicious, featuring baked Brie topped with a spicy salsa made of cranberries, Granny Smith apple, jalapeño and fresh cilantro.
12 Days of Delicious Christmas Dishes


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Pinehurst Resort tweets beautiful iconic photo with a twist
Give Pinehurst Resort credit. They've taken a situation that certainly doesn't help business - and turned it into a way to celebrate an icon and their venue.
With a snow storm blasting many areas of the Southeast, Pinehurst was covered in a somewhat rare but still significant snowfall. So what'd they do? They shared a great photo of their most celebrated champion, Payne Stewart.
Payne, dressed in #snow. @MattGinellaGC @GCMorningDrive @garywilliamsGC @johnkim pic.twitter.com/74ZlPQ9M8n
— Pinehurst Resort (@PinehurstResort) January 29, 2014
Pinehurst Resort has been great at sharing their news and images via their blog. You can check it out here.
The Latest
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- Description
Set sail right in the comfort of your home with this Polyfiber covered 2-piece sofa set. It's light and airy surface features modern designed furnishings of clean lines and bold structures. It also includes striking accent pillows and a cocktail ottoman. Available in khaki and slate black.Includes
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What is JotForm?
JotForm is a free online form builder which helps you create online forms without writing a single line of code. No sign-up required.
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How can I delete an account?Asked by lindah on August 16, 2014 at 10:44 AM
Realized about I got started that I need a different user name for this project. Any way I can copy the current information to a different Username and then delete this one?
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Food, Cooking Media & Bloggers: Stain-Proof Chef Jacket and Apron Now Available for Review
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PhoneArena Awards 2012: Game-changing Product
0. phoneArena posted on 04 Dec 2012, 11:44
Every once in a while, a new product steps in and changes everything. Its impact on the mobile industry is so significant that it causes competing companies to rethink their strategies if they are to survive on the market. And that's when things get really interesting!...
This is a discussion for a news. To read the whole news, click here
1. drahmad (Posts: 480; Member since: 20 Aug 2011)
they are not game changers, they "game on" products
15. SuperAndroidEvo (Posts: 4888; Member since: 15 Apr 2011)
What about setting a new standard, like 1080p HD screens on mobile phones?
That is game-changing, don't you think? For Sharp to release a 1080p HD display at 5" is a TRUE definition of "game-changing", to me at least. Do you know how hard it is to fit 2,073,600 pixels in a small 5" diagonal area? Why did PhoneArena.com miss this on their best innovations of 2012 & now on their game-changing product of 2012?
Sharp is responsible for the technology, BUT HTC brought it to the world on their BEAST Droid DNA/J Butterfly smartphones.
24. kickgolfer (Posts: 69; Member since: 03 Dec 2012)
HTC's not a game changer. They're just the first buyer for this panel from Sharp. It's 1080p screen is very nice(IMO, best screen out, period) but upgrade from 720p to 1080p was expected. LG, japandisplay, Samsung, other taiwanese &japanese OEMs already all posses the manufacturing ability of 5" 1080p lcd panels, it's just matter of when.
Samsung's even working on 1080p SAMOLED panels, which no one in the business is come close to/able to produce at the moment. I hear even Samsung having trouble mass producing it right now.
27. SuperAndroidEvo (Posts: 4888; Member since: 15 Apr 2011)
Can you imagine a beautiful 5.5" or 6" 1080p RGB Super AMOLED display? Can you say Samsung Galaxy Note III? lol
39. kickgolfer (Posts: 69; Member since: 03 Dec 2012)
I love my Galaxy Note 2's display but I know it's not the best out there now. I give that title to Sharp's 5" 1080P SLCD3(HTC's Droid DNA/Butterly J) but like I said before everyone's going to have simiar displays on their high-end devices. All going to be 1080p and sweet. IPS panels are really great for being smartphone displays and more but OLED does have it's strong points, too.
As most of you here already know, Samsung went with the pentile type on their first Galaxy S and Nexus but used the RGB type on their second generation of Galaxy S line. Before the release of this year's most popular phone, the Samsung's Galaxy S3, Samsung and Google collaborated and released the Samsung Galaxy Nexus S with pentile display. Even though it had pentile display, it did have improvements over Galaxy S2's RGB type display. Samsung used the same type of pentile display on their latest Galaxy S device but still made a lot of improvments over Galaxy Nexus S's display. I know the Note 2 has RGB type display but I'm just amazed by how quickly Samsung's evolving and they're doing it all alone right now. Samsung's OLED tech should be at least 2 yrs ahead of it's competition. I can't wait to see the upcoming S4's beautiful 4.99" SAMOLED Plus display. White should be whiter than now and colors should be more natural and bright.
40. kickgolfer (Posts: 69; Member since: 03 Dec 2012)
I love gaming on my Galaxy Note 2 but gaming or watching videos and surfing web on 5.6~6" 1080P SAMOLED Plus display with improved color saturation, balance and whiter white would be pure epic.
29. gwuhua1984 (Posts: 1237; Member since: 06 Mar 2012)
No no, the phone really is a game changer... Just not innovation. Compared to the Nexus, it's just a bit short of a game changing product.
31. Berzerk000 (Posts: 4275; Member since: 26 Jun 2011)
Not really game-changing, it was an expected evolutionary advancement. Though, it is beautiful, it isn't game-changing, considering that this is just going to be the new standard for flagships in 2013. HTC was just first to make a consumer model with a 1080p display, not a game-changer, it just set a new standard.
My definition of game-changing is when a company outs a product that will completely turn the tides for the company and changes that specific industry as a whole. 5" 1080p displays are only evolutionary, not revolutionary.
33. Zero0 (Posts: 592; Member since: 05 Jul 2012)
A $300 quad core, 2GB RAM, 312 ppi, 4.7 inch, always updated smartphone sounds pretty game-changing to me. 1080p is a nice upgrade, but not earth-shattering. A half-price flagship, that's going to shake things up.
42. Nadr1212 (Posts: 741; Member since: 22 Sep 2012)
I can see why They didn't mention the Google Nexus 4,
It doesn't even deserve an 8
45. Jasonhunterx (Posts: 111; Member since: 20 Jul 2011)
Lately you've been really trolling with this 1080P junk we get it it looks great but its surely not a game changer its a cool new improvement but not game changer you can buy a high end and high end phone for 550$ that's a game changer last year we wouldn't be able to say those words now the game is changed let the value/price war begin!
52. infinitemethod (Posts: 151; Member since: 04 Sep 2012)
So the Nexus 10 display at its high res gets no love? Very few actually care about display specs anymore. People like you and me do but the vast majority of people probably cant even tell a difference.
I think the real game charger that the Nexus line is pricing. Instead of charging a premium for storage like Apple does, offering a better display, better hardware and an OS that is light years ahead of iOS, theyre undercutting Apple/MS and taking away marketshare.
28. Pings (Posts: 304; Member since: 19 Dec 2008)
I agree. The Google Nexus family are "game on" products. I would say Android 4.0,4.1,4.2+ are true game changers.
2. Aaron_R96 (unregistered)
Agree! Google went all out with kick-ass features on all of the 3 Nexus' they announced this year, using price, form and just pure brilliant technology in them all.
In my opinion, it will be especially the pricing of the Nexus 4 which will (hopefully) change the smartphone market for good next year! I do worry about other Android OEM's whose profit margins are already small - and worry this could lead to Apple to pull further ahead in terms of total revenue however..
3. nwright94 (unregistered)
I agree. Here come the complaints about the galaxy note II not being your pick though :/
4. Aaron_R96 (unregistered)
Experienced this on the innovation award yesterday didn't we? :/ ready for round 2...?
6. nwright94 (unregistered)
Yeah that's what I'm referencing haha. He will probably be in here flooding the comments shortly.
18. SuperAndroidEvo (Posts: 4888; Member since: 15 Apr 2011)
I just WANT PhoneArena.com to at least mention the 1080p HD screens that will be the standard for years to come.
Seems we are going to repeat yesterdays innovation fiasco all over. lol
25. Aaron_R96 (unregistered)
Yeah i agree! I added a comment tonight on the innovation one agreeing with you haha! Can't wait for it to be the new standard for high end next year, DNA, Sony Odin & Yuga, Galaxy S4 (presumably) etc etc
26. SuperAndroidEvo (Posts: 4888; Member since: 15 Apr 2011)
Exactly. Let the 1080p WARS begin! lol
49. XiphiasGladius (Posts: 813; Member since: 21 Aug 2011)
Hey man, you already have the DNA? if so then how good is adreno 320 in processing all that pixels?
54. SuperAndroidEvo (Posts: 4888; Member since: 15 Apr 2011)
The HTC Droid DNA with the quad-core Qualcomm Krait S4 Pro with the Adreno 320 is just EPIC. The chipset is SO powerful that I can under clock it from 1.5GHz to 1.0GHz & the machine runs incredibly smooth.
The 1080p SLCD-3 is so frugal with its power consumption that the battery life is just incredible. The phone with a 5" 1080p HD screen & quad-core processor is almost 3 times more efficient that the Samsung Galaxy Nexus.
I got 2 days 16 hours on a single charge with about 3 to 3 and half hours of screen on time. That is with NORMAL usage. My GNex on normal usage would last 1 day 3 hours with about 1 hour & 6 to 12 minutes of screen on time.
I can honestly say the HTC Droid DNA is GREAT with battery usage. Also pushing all those pixels is NO problem.
55. remixfa (Posts: 14605; Member since: 19 Dec 2008)
Welcome to the fuuuuuuuuuuuuuutuuuuuuuuuuuuuuuuuuure.. The DNA doesnt have 4.2 on it. You can kiss some of that battery life goodbye when it gets updated. :(
I run my nexus4 at 800mhz. Give it a shot. I still get no performance drops. and the difference in battery life from stock speeds is tremendous.
5. gazmatic (Posts: 795; Member since: 06 Sep 2012)
yes. high specs but low price... that is what I'm talking about... hopefully the price will drop even further... I still say that $600 for a 64gb phone is too much...
7. MeoCao (unregistered)
My picks are:
1. Nexus 7: High specs, low price concept has changed the landscape for Android tablets and great value for consumers. It also helped hugely strengthen Google Play.
2. Nexus 4: Affordable price and successful direct selling to consumers threaten to disrupt old sales model where carriers decide everything.
3. HTC Butterfly J/Droid DNA: Set new standard for phone screen.
11. lzsbleach (Posts: 155; Member since: 20 May 2012)
HTC butter fly is not a game changer but its a decent phone. The Note 2 would be considered a game changer with its use of S Pen, multi-app use and changing the phablet market from a Niche market to mainstream. The Note series gave birth to the HTC butterfly which is the first phone with a 1080p screen (WOW sarcastically) but they didnt even set a trend because 1080p screens already been in development before the butterfly came out.
16. MeoCao (unregistered)
Note 2 is a great phone but it changes nothing. It continues the business model of OG Note. OG Note was a game changer.
On the other hand HTC Butterfly J has changed the thinking of people about screen resolution. most people were fooled by Apple's Retina marketing ploy according to which anything higher than iPhone's pixel density is waste.
HTC Butterfly J has proved that is wrong and set new trend that top phones must follow.
30. richardyarrell2011 (banned) (Posts: 510; Member since: 16 Mar 2011)
The Samsung Galaxy Note 2 is not a phone it's the ultimate productivity/all purpose device ever made. If you want a phone by the Nexus 4, Htc Dna, Droid Razr Maxx Hd. The Samsung Galaxy Note 2 is something all together on it's own level.
53. infinitemethod (Posts: 151; Member since: 04 Sep 2012)
I disagree. Sure the Note 1 was the first of its kind, however, Samsung managed to add .5" all the while making it less wide and slimmer. Now that in and of itself isnt a huge deal, but it most definitely makes me more appealing for such a big device.
Having full hd display is nice but that does nothing for me. Features and functionality are game changers. Go try out the Galaxy Note 2, s mutli window feature and tell me that that isnt an amazing feature, not to mention a very useful one.
20. SuperAndroidEvo (Posts: 4888; Member since: 15 Apr 2011)
I don't think you get how difficult it is to fit OVER 2 million pixels in a small 5" diagonal screen. I am sorry my good friend but that IS a game changer. It's actually the new world standard for high-end smartphones/phablets.
Apple was always given credit for their "Retina" display when in fact it was LG who was responsible NOT Apple. So if it works for Apple then it works for HTC.
HTC WAS the first with a TRUE 1080p HD SLCD screen on a mobile device even though the 1080p display is Sharp's doing. So HTC is due some props.


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Doucette's is proud to offer new rental items!
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Some days I put myself in an amazing person's shoes so I can visualize accomplishing feats of greatness. This week, I imagined myself Serena Williams, until she did the splits and popped right back up. My mind couldn't get beyond the pain.
Month: August 2014
First Impressions
The "Fear" Talk
My mother is scared of almost everything, which means I spend a lot of time as an unpaid therapist.
This week's irrational fear has been ebola.
The rational fear has been heat stroke.
I'm pretty sure tomorrow's fear will be an otter attack.


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Brother Jack
Just Some Nut
Join Date: Jan 2008
Location: 2300 A.D.
Posts: 8,414
Re: Tom Hardy as Bane XXX
Originally Posted by
I agree with everything you said here except that I don't think we can say for sure that Alfred's intel on Bane is false. He says he was born in raised in the prison and no one knows how he escaped the Pit, only that he was trained by Ra's once he got out. We know the second part is true, so it's very possible that the first part is too. As you said, Talia seems to be completely off the radar of the authorities (the CIA was looking for Bane, not Talia) so I'm doubting that somehow their two stories got confused.
I was talking about him escaping the pit specifically. Bane had the reputation of being the one that escaped the pit and we are led to believe this is true until Talia is revealed.
It was the look on their faces...
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Once a school governing council endorses a decision to close an OSHC service, they must meet specific requirements.
Responsibilities of the school governing council
The school governing council must ensure that:
- families are provided with information about alternative school age care options in the community, where these options are available
- the community is notified
- a notification of closure to the Australian Government is submitted at least 42 days prior to closure
- a notification of surrender of service approval form is submitted to the Education and Early Childhood Services Registration and Standards Board of South Australia.
Responsibilities of the principal
The principal must make sure that:
- the school governing council minutes where the decision to close the service was endorsed, and the education director's endorsement, are decd.oshc [at] sa.gov.au (emailed to the department's OSHC unit)
- funds are available to meet all staff entitlements prior to closure of the service
- company 4 remains open until 30 June after group certificates have been completed, or to 31 December if the transfer is after the end of the financial year.
Out of school hours care unit
Phone: 8226 6427
Email: decd.oshc [at] sa.gov.au


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Kean University
October 15, 2010 by USA Post
Kean University, Concert Artist Program of Kean University will celebrate the 200th anniversary year Chopin November 4 to 20 hours Gabriela Martinez, piano; Joanna Frankel, violin, and Carrie Stinson, cello will perform several pieces by Chopin, including his Sonata for Cello and Piano Trio. The concert took place in Kean Hall, located on Kean's main campus at the entrance to Green Lane just off of 1000 Morris Avenue, Union.
Concert Artist program brings world-renowned musicians performing on the faculty of the Conservatory of Music. While maintaining a successful career as a professional artist, artists of this solo concert, chamber music and recitals of jazz Kean, teach private studio week for Kean students, and conduct master classes and other community outreach services in public schools and colleges. Some artists together also sets the coach and teach the classroom related to their specialties. Tickets are 25 and are available by calling 1-908-737-SHOW (7469), and online at www.keanstage.com.
Please feel free to send if you have any questions regarding this post , you can contact on
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Cole Veterinary Hospital at Legends Ranch
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- 4 Posts. Joined 5/2016
- Points: 10
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Welcome to the forum Mike!
Glad to have you aboard.
Sounds like your butt was under cooked. We usually figure 2 hours per pound at 225. But always go by meat temp.
Next time let it go until 205 or until a probe goes in with little to no resistance.
Check it in several places and make sure it's done everywhere.
The bone should just slide out clean.
Welcome Mike! I too am an FNG and you hit it on the head, this whole thing is a learning experience. You will find a wealth of knowledge from the fine folks here on SMF. Everyone is amazing and very helpful.
Thank you for your service Brother in Blue, stay safe out there.
I don't think the type of charcoal made a difference. It is hard to maintain a steady temp on an inexpensive smoker like yours.
That's because the metal is so thin. I think you did a great job of controlling the temps. Next time I would leave the water bowl in but leave it dry. This will let the temps get a little higher without using so much fuel. You can also put sand in the water bowl & it will act like a heat sink & help keep the temps more stable.


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Katrina Kaif was in Delhi recently, to promote her upcoming film Fitoor. The actress went an extra mile to pray for the success of the movie at Ajmer Sharif Dargah. Katrina Kaif was looking beautiful without any makeup, in her white salwar and kurta.
At the holy shrine, Katrina Kaif made the offering of flowers and chaddar.
Not many people know that before the release of her film, Katrina Kaif visits Khwaja Moinuddin Chishti, which is located in Ajmer, Rajasthan. She believes that it brings good luck to her movies. The actress is a regular at this shrine and was spotted here praying for the success of Dhoom 3 and Phantom in the past.
Katrina Kaif is working with Aditya Roy Kapur in Fitoor. The success of the movie is equally important for both the actors as Fitoor will decide their future in Bollywood. Some insiders say that Katrina's role is very strong in the movie and she will definitely win critical acclaims this time for her acting.
Talking about her movie Fitoor, Katrina Kaif told a leading news agency, ''It's basically about a mother who has been extremely jilted by her lover and has become very bitter. And as a result, she is almost brainwashing and manipulating her daughter and kind of having a wrong way on love and life.''
"Maybe in a certain way, she is trying to protect her from the same heartbreak which love can sometimes bring and some way encourage her to take revenge on men in general for the hurt," she added.
Sultan In Trouble Again? Anushka Sharma Slaps Salman Khan On The Movie's Sets


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CYR61/CCN1 and WISP3/CCN6 are chemoattractive ligands for human multipotent mesenchymal stroma cells
CCN-proteins are known to be involved in development, homeostasis and repair of mesenchymal tissues. Since these processes implicate recruitment of cells with the potential to be committed to various phenotypes, we studied the effect of CYR61/CCN1 and WISP3/CCN6 on migration of human bone marrow derived mesenchymal stroma cells (MSCs) in comparison to in vitro osteogenic differentiated MSCs using a modified Boyden chamber assay.
CYR61 and WISP3 were purified as fusion proteins with a C-terminal Fc-tag from baculovirus infected SF21 cells using protein G sepharose columns. CYR61 and WISP3 stimulated cell migration of undifferentiated MSCs in a dose-dependent manner. CYR61 and WISP3 had similar effects on committed osteogenic precursor cells. Checkerboard analysis revealed that CYR61 and WISP3 stimulated true directed cell migration (chemotaxis) of MSCs and committed osteogenic precursors. In MSCs the chemotactic activity of WISP3 but not CYR61 was mediated through integrin
Our results indicate that CYR61 and WISP3 can function as soluble ligands transmitting chemotactic signals to human MSCs but differ in the involvement of integrin
Members of the cysteine-rich 61/connective tissue growth factor/nephroblastoma overexpressed (CCN1-3) family of genes (CCN-family) have been reported to be involved in central cell-biological processes such as cell adhesion, migration, proliferation, differentiation and survival
Various CCN-proteins have been implicated in chondrogenesis and bone formation as well as angiogenesis during development and adult life
The observation that mutations in WISP3/CCN6 lead to progressive pseudorheumatoid dysplasia (PPRD) and polyarticular juvenile idiopathic arthritis, diseases that are associated with cartilage growth plate defects and/or loss of adult articular cartilage indicates that this CCN-protein might be related to the preservation of cartilage integrity
The aim of this study was to establish recombinant expression of WISP3/CCN6 and to quantify possible chemotactic effects on primary human MSCs in comparison to CYR61/CCN1. Our results indicate that both CCN-proteins are chemoattractive ligands for human mesenchymal stroma cells before and after osteogenic commitment and that the effect of WISP3/CCN6 but not CYR61/CCN1 on cell migration of MSCs is mediated by the integrin receptor
Silver staining and western blotting revealed the purity of the recombinant WISP3-Fc protein (Figure
Figure 1
Silver staining and western blot of recombinant WISP3-Fc
Silver staining and western blot of recombinant WISP3-Fc. To check the purity of WISP3-Fc SDS gel electrophoresis followed by silver staining and western blotting were applied as described in materials and methods. a) western-blotting analysis, b) silver stained SDS gel. Size of the fusion protein is 72 kDa.
Human MSCs used for the chemotaxis assays did not express markers of differentiated osteoblasts (osteocalcin), chondrocytes (collagen type II) or adipocytes (leptin). Immunostaining of MSCs showed that
Full length CYR61 and WISP3 induced a dose-dependent migratory response in human bone marrow derived MSCs through gelatin-coated chemotaxis filters (Figure
Figure 2
CYR61 and WISP3 stimulate migration of human MSCs
CYR61 and WISP3 stimulate migration of human MSCs. CYR61/CCN1 and WISP3/CCN6 stimulate migration of human MSCs in a dose dependent manner. Results are presented as absolute numbers of migrated cells from six independent experiments, measured in quadruplicate each (mean ± SD, * p < 0.05, ** p < 0.01 versus negative control in paired t-test).
Figure 3
CYR61 and WISP3 stimulate migration of osteogenic differentiated MSCs
CYR61 and WISP3 stimulate migration of osteogenic differentiated MSCs. CYR61/CCN1 and WISP3/CCN6 stimulate migration of osteogenic differentiated human MSCs in a dose dependent manner. Results are presented as absolute numbers of migrated cells from six independent experiments, measured in quadruplicate each (mean ± SD, * p < 0.05, ** p < 0.01 versus negative control in paired t-test).
Since the recombinant proteins both contained the IgG Fc-tag it was necessary to test whether the Fc-tag itself influenced cell migration of MSCs or dMSCs. As shown in Figure
Figure 4
The Fc-tag does not influence migration
The Fc-tag does not influence migration. The Fc-tag has no influence on migration of human MSCs and osteogenic differentiated MSCs up to 2 μg/ml. Results are presented as absolute numbers of migrated cells measured in quadruplicate (mean ± SD) from one representative experiment out of two independent experiments.
To rule out the possibility that CYR61 and WISP3 induced chemokinesis, and not directed cell migration, a checkerboard analysis was performed with the most effective concentration of 2 μg/ml. These results (Table
Table 1
Checkerboard analysis for migratory effects of CYR61/CCN1 and WISP3/CCN6.
negative control
14.3 ± 2.9
16.3 ± 3.7
55.5 ± 6.5
40.3 ± 4.0
18.8 ± 4.4
15.0 ± 2.9
43.5 ± 3.2
30.8 ± 3.3
12.0 ± 2.2
13.5 ± 4.2
Checkerboard analysis for migratory effects of CYR61/CCN1 and WISP3/CCN6 with human MSCs and in vitro osteogenic differentiated MSCs (dMSC). Results are presented as absolute migrated cells (mean ± SD of one quadruplicate determination). 2 μg/ml CYR61/CCN1 or 2 μg/ml WISP3/CCN6 were added only to the lower compartment (lower) or to both the upper and lower compartment (upper&lower) of the chemotaxis chamber to eliminate the concentration gradient.
Preincubation of MSCs with the integrin
Figure 5
Figure 6
Normal mouse IgG does not influence MSC migration to CYR61 or WISP3
Normal mouse IgG does not influence MSC migration to CYR61 or WISP3. Preincubation with normal mouse IgG does not influence the response of MSCs to 2 μg/ml CYR61 or 2 μg/ml WISP3. Results are presented as absolute numbers of migrated cells measured in quadruplicate (mean ± SD) from one representative experiment out of two independent experiments.
A chemotactic effect of CYR61/CCN1 has been previously described for fibroblasts, alpha smooth muscle cells, endothelial cells and the murine mesenchymal stem cell line C3H10T1/2
This is the first report that beside full-length CYR61/CCN1 also full length WISP3/CCN6 acts as a soluble factor stimulating migration of human bone-marrow-derived MSCs and dMSCs in a dose-dependent manner. Moreover, the stimulation could be specified as chemotaxis and not chemokinesis and, thus, may contribute to site-directed cell recruitment in vivo. These results confirm the biological activity of both recombinant expressed proteins. 2 μg/ml of the recombinant CCN-proteins in which the Fc-tag accounts for nearly half of the protein lead to about 70–80% of migrated cells compared to 100 ng/ml rhIGF-I. The concentrations needed for a similar chemotactic effect of these matricellular proteins were therefore markedly higher than for other growth factors but they were comparable to concentrations of CCN-proteins known to elicit cell-biologic responses in previous studies. The characteristics as matricellular proteins in tightly binding to heparan sulfate proteoglycans and distinct integrin receptors as well as the solubility of these proteins may be related to this point. For chemotactic processes along far distances proteolytic fragments of these domain structured proteins might be involved, which remains to be tested by functional domain bashing analysis in future experiments. In this context it might be relevant that IGFBP5 has a direct chemotactic effect on human MSCs
We have previously shown that in vitro differentiation of MSCs to the osteogenic phenotype by dexamethasone, ascorbic acid and ß-glycerophosphate mostly led to a chemotactic response very similar to that of primary osteoblasts
Our in vitro data show that recombinant CYR61 and WISP3-proteins both stimulate directed migration of human MSCs and committed osteogenic precursor cells. The chemotactic activity of these two members of the CCN-protein family differently involves integrin
Cell Culture
With informed consent, following the guidelines from the ethics committee of the University of Ulm, bone marrow samples from eight donors (age 20 to 39 years) were plated and cultured in Dulbecco's modified eagle medium (DMEM; Biochrom, Germany) containing 10% FCS as described previously
Osteogenic differentiation of MSCs and analysis of gene expression
Medium for osteogenic differentiation, containing DMEM with 0.1 μM dexamethasone, 10 mM ß-glycerophosphate and 50 μg/ml ascorbic acid, was changed every third day as described
Cloning for WISP3 expression
The open reading frame for WISP3 was amplified from a plasmid (IMAGp956O19142) obtained from the Resource Center Primary Database (Berlin, Germany). Primers corresponding to the translational start site (including the KOZAK Sequence of the WISP3 cDNA sequence) and the stop site (the primer lacked the stop codon) contained restriction sites at the 5'end (
Transfection of insect cells and expression of full-length CYR61 and WISP3 proteins
SF-21 caterpillar (lepidopteran) derived cells were used to express recombinant WISP3-protein (rWISP3). The recombinant vector was mixed with linearized DNA that contained a modified
The purification of the Fc-tag was done in parallel using similar procedures. The Fc-domain of human IgG was amplified from a PC3 expression vector (kindly provided by Dr. Pascal Schneider, University of Lausanne, Switzerland) by PCR using primers containing EcoRI and NotI restriction sites and subcloned into the transfer vector pBakPak 8 (Clontech, Saint-Germain-en-Laye France). Further virus amplification and protein purification was done essentially as described above for the CCN-proteins.
Chemotaxis Assay
Cell migration was analyzed by a modified Boyden chamber assay, using a 48-well micro-chemotaxis chamber (NeuroProbe Inc., Baltimore, MD, USA) and polycarbonate filters with 8 μm pores (Whatman Biometra, Göttingen, Germany), as described previously
SDS gel electrophoresis, silver staining and western blotting
SDS gel electrophoresis, silver staining and western blotting essentially were performed as described previously
The chemotaxis experiments on dose-dependent effects of CYR61 and WISP3 were performed in quadruplicate, for MSCs and osteogenic differentiated MSCs (dMSCs) in independent experiments with cells from six different donors, and the mean value for each donor was used for statistical analysis. Experiments with integrin-blocking antibodies were done in quadruplicate determinations with cells from three different donors each. Results are presented as mean ± standard deviation. The significance of differences in chemotactic responses compared to the negative control was determined using Student's
Authors' contributions
NS synthesized recombinant CYR61 and WISP3 and participated in drafting the manuscript, RS established recombinant synthesis of CYR61 and WISP3, JF was involved in the experimental design of chemotaxis experiments and data evaluation, TM aspired bone marrow samples and contributed to the establishment of MSC cultures, FJ participated in data interpretation and drafting of the manuscript, RB designed the study and drafted the manuscript. All authors read and approved the final manuscript.
The authors would like to thank the German Society for Orthopedics and Orthopedic Surgery for financial support within the "Network of Regenerative Orthopedics". We are grateful to Dr. Pascal Schneider, University of Lausanne, Switzerland for providing an Fc-tag containing plasmid and to Mrs. Susanne Jatzke and Mr. Giovanni Ravalli for expert technical assistance.
Cyr61, a product of a growth factor-inducible immediate-early gene, promotes cell proliferation, migration, and adhesion
The CCN family of angiogenic regulators: the integrin connection
The CCN family: a new stimulus package
Mesenchymal stem cells reside within the connective tissues of many organs
Multilineage mesenchymal differentiation potential of human trabecular bone-derived cells
Mesenchymal stem cells
Human bone marrow stromal cells express a distinct set of biologically functional chemokine receptors
Migration of bone marrow and cord blood mesenchymal stem cells in vitro is regulated by stromal-derived factor-1-CXCR4 and hepatocyte growth factor-c-met axes and involves matrix metalloproteinases
The migration of bone marrow-derived non-hematopoietic tissue-committed stem cells is regulated in an SDF-1-, HGF-, and LIF-dependent manner
Bone morphogenetic protein-2 but not bone morphogenetic protein-4 and – 6 stimulates chemotactic migration of human osteoblasts, human marrow osteoblasts, and U2-OS cells
Basic fibroblast growth factor controls migration in human mesenchymal stem cells
BMP-2, BMP-4, and PDGF-bb stimulate chemotactic migration of primary human mesenchymal progenitor cells
To go or not to go: Migration of human mesenchymal progenitor cells stimulated by isoforms of PDGF
IGF-I and IGF-II stimulate directed cell migration of bone-marrow-derived human mesenchymal progenitor cells
Vascular endothelial growth factor stimulates chemotactic migration of primary human osteoblasts
VEGF-A and PlGF-1 stimulate chemotactic migration of human mesenchymal progenitor cells
Expression, purification, and functional testing of recombinant CYR61/CCN1
CCN1/Cyr61 Is Regulated by the Canonical Wnt Signal and Plays an Important Role in Wnt3A-Induced Osteoblast Differentiation of Mesenchymal Stem Cells
Mutations in the CCN gene family member WISP3 cause progressive pseudorheumatoid dysplasia
Wnt-1-inducible signaling pathway protein 3 and susceptibility to juvenile idiopathic arthritis
WISP3-dependent regulation of type II collagen and aggrecan production in chondrocytes
WISP-3 functions as a ligand and promotes superoxide dismutase activity
Differential expression of CCN-family members in primary human bone marrow-derived mesenchymal stem cells during osteogenic, chondrogenic and adipogenic differentiation
The fate of circulating osteoblasts
CYR61 stimulates human skin fibroblast migration through Integrin alpha vbeta 5 and enhances mitogenesis through integrin alpha vbeta 3, independent of its carboxyl-terminal domain
The angiogenic factor cysteine-rich 61 (CYR61, CCN1) supports vascular smooth muscle cell adhesion and stimulates chemotaxis through integrin alpha(6)beta(1) and cell surface heparan sulfate proteoglycans
CYR61, a product of a growth factor-inducible immediate early gene, promotes angiogenesis and tumor growth
Fisp12/mouse connective tissue growth factor mediates endothelial cell adhesion and migration through integrin alphavbeta3, promotes endothelial cell survival, and induces angiogenesis in vivo
CCN3 (NOV) is a novel angiogenic regulator of the CCN protein family
Integrin-dependent functions of the angiogenic inducer NOV (CCN3): implication in wound healing
Pro-angiogenic activities of CYR61 (CCN1) mediated through integrins alphavbeta3 and alpha6beta1 in human umbilical vein endothelial cells
Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers
Effects of dexamethasone on proliferation, chemotaxis, collagen I, and fibronectin-metabolism of human fetal lung fibroblasts
Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor


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I am 13 years old and in middle school. I have liked this guy for AGES and he finally asked me out last year and I said yes. I had this one annoying friend that was my excuse to hang out with him. She left the school but I still hung out with him for a few months (he would ditch his friends and hang out with me) but then one day he stopped and he hasn't ever hung out with me since (I tried to hang out with him once but he just talked to be for like 15 minutes and then kinda forgot I was there and talked to his friend) Everytime I walk past him, we both look at each other weirdly and I don't know why... But we just walk away from each other... We only have tiny little conversations but they are really nice and funny :) I don't know if he still likes me or not? I have some friends saying that he probably doesn't like me anymore but he might be thinking the same thing!!! It is so complicated and I overthink things!!! Sorry this is so long but PLEASE HELP ME!!!
Most Helpful Guy
Pull him aside and give him a hug. Tell him u miss hanging with him.11|00|0
Thanks. But I don't really know how... He hardly ever leaves his friends... I have seen him at school every day and he has seemed pretty upset and I don't really know why? We have maths together and I always find him looking at me but pretends he wasn't (He doesn't look away he just pretends he was looking above me at info)
You just gotta find the time to reach out. Maybe something is troubling him. Anyway I'm sure he'd be happy to be reminded that u care for him.


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George Clooney Liked to Mess with Walter Cronkite: Late Last Night
Last night, George Clooney stopped by The Late Show to talk about pranking a 90 year-old Walter Cronkite at his Italian villa to the point of getting him to (drunkenly) jump in his lake.
Hugh Jackman appeared on The Daily Show to talk about getting punched in the stomach by Sugar Ray Leonard, and how all the non-dangerous movies he tries to get involved with always get canceled.
Will Arnett showed up on Jimmy Kimmel Live! to reveal that his mother was clearly not a fan of Arrested Development, and to find out that he inadvertently insulted just about every member of the Jimmy Kimmel Live! crew through different scenes of his new sitcom, Up All Night.
Finally, Evan Rachel Wood visited Late Night to talk about getting her tooth knocked out on her birthday, and the crowd of screaming girls perpetually following Ryan Gosling.


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"You need to play it," 5th Cell producer Caleb Arseneaux told me. That's why Hybrid will not only have a beta, but remain an XBLA exclusive for the time being. "You need to play it to discover its charm, and so XBLA has free demos. That's a huge thing: you don't need to risk anything. You get in, you play, and we'll have a counter or whatever that will count down before you have to decide if you'll buy it. That's enough for us, because what's going to sell it more than anything else is the gameplay itself. So, yeah, we're an exclusive XBLA title right now."
As soon as I jumped into a game and started darting around, I immediately fell in love with the quick, twitchy gunplay. The customization options, though fairly uninspired, added a layer of depth to the affair – guns, individual character perks and grenade modifiers are all available. Some of the more ingenious options included a hacking grenade, which turns enemy drones against their masters if they're caught within the blast radius, and team-based buffs such as increased weapons damage or faster ability cooldowns.
Hybrid features a persistent online world that changes constantly, based on the outcome of battles. Battles take place across continents, which are broken down into regions – once a side has won a region, it's locked out. Each region also awards the side who controls it a special modifier, and it takes only a glance to see which side is dominating a particular region.
Once you boot up Hybrid, you have to pick a side: Paladin or Variant. Once chosen, you're locked in for the long haul and fight in regions to try and capture the area and, eventually, the continent. "Everyone constantly contributes for their faction," Arseneaux tells me. "That is how players will complete the world map part of the game. They're all contributing to the same war effort. We estimate players will lock out the entire world map in two-to-four weeks. One day you may see the Paladins have locked out North America, and another day you may see the Variant has Asia." Hot Zones will show where the two factions are neck-and-neck in fully claiming an individual region.