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Read Mark Twain free essay and over 86,000 other research documents. Mark Twain. Did you know that Mark Twain was actually pseudonym? Do you know what his real name was. "The Awful German Language" is an 1880 essay by Mark Twain published as Appendix D in A Tramp Abroad. The essay is a humorous exploration of the frustrations a native. AbeBooks.com: The Complete Short Stories and Famous Essays of Mark Twain: Ships from the UK. Shows some signs of wear, and may have some markings on the inside. Famous People term papers (paper 20176) on Mark Twain : Charles Goodyear was born in New Haven, Connecticut on December 29, 1800 to Amasa and Cynthia. Papers on Twain Essays term papers. A 3 page essay that discusses Twain's Tom Sawyer. Mark Twain's most famous book is Huckleberry Finn and critics agree that. In Advice to Youth, a talk he delivered to a group of young girls, Mark Twain turns the conventional moral lecture on its head.
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Christened as Samuel Langhorne Clemens, Mark Twain was born on November 30, 1835 in the small river town of Florida, Missouri, just 200 miles from Indian Territory. Samuel Clemens/Mark Twain 1835-1910 Samuel Clemens was born on November 30, 1835 in Florida, Missouri, the sixth of seven children. At the age of four, Sam and his. Free Mark Twain papers, essays, and research papers. Get this from a library! The complete short stories and famous essays of Mark Twain [Mark Twain. 1000 Mark Twain - Known for his razor sharp wit and ever-present cigar, Mark Twain is one of America's most respected and appreciated humorists. In addition to his. Essays and criticism on Mark Twain's The Adventures of Huckleberry Finn - The Adventures of Huckleberry Finn, Mark Twain. Best of Mark Twain Considered by some to be the real founder of American Literature, warts and all. List your favorites! All Votes Add Books To This List. 1: The.


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My name is Ákos Lumnitzer. I was born in Hungary a few years ago and moved to Australia in 1985. My name actually means "White Falcon" believe it or not! Read about my name here (name #5): And I find the meaning rather appropriate. As for pronouncing it, well, it is really not all that hard once you know.
I am totally enchanted by birds of prey. They are my favorite study (for my own knowledge) and photography subject. If I am not out chasing birds of prey, will most likely find me deep in a swamp photographing waterbirds or atop a rocky mountain seeking out elapid snakes and other reptiles. Although, you may also find me alone at night in some remote forest where I look for the elusive and incredible fauna that Australia is so extremely fortunate to call its own. In fact, nocturnal solitude in the bowels of a forest is my most favourite place to be.
However, in between the photography outings there has to be some sort of reality and earn a living somehow. Nothing is more important to me than my little family. My wonderful wife, who is currently undertaking full time veterinary science studies at Sydney Uni, my five wonderful cats, three gorgeous dogs and an old horse called Buddy. And on top of that we try to help injured wildlife through WIRES and foster dumped cats with our friends from Cat Rescue. So my life is busy and I love it. But once I am free to be outdoors, my calling is mother nature and her creatures. This blog is to share my love and passion with anyone mad enough to read my humble ramblings.
Feel free to visit my personal website A MATTER OF LIGHT
Best regards and wish you wonderful light for your photographic journey

subset: commoncrawl


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Look who we spotted this weekend at the Africa Aerospace and Defence 2014 show at the Waterkloof Airforce Base in Centurion. His name is Parabot, and he stands almost 10 metres tall! Parabot is a Transformer brought to life – to wildlife, to be exact. He is made from an armoured vehicle, and is a symbol of the fight against rhino poaching.
Today is World Rhino Day (22 September), and Parabot was made by Africa's largest privately-owned defence and aerospace company, Paramount Group, to raise awareness of the efforts that the defence industry is playing in preserving our magnificent rhinos.
Behold, the Parabot! Kids will love him.
#Parabot – the stats
- Parabot's concept and design is based on Paramount's Group largest armoured vehicle, the Mbombe 6
- Weight:1 tonne
- Height: 9.7m
- Hours to build: 600 man hours
- Built by: CFX, a famous Film Effects and Fabrication Company based in Cape Town
- Mission: Raise awareness and protect Africa's wildlife from poaching
Rhino stats
According to the Department of Environmental Affairs:
- 769 rhinos have already been poached in 2014
- 227 arrests associated with rhino poachings have been made in 2014
- The Kruger National Park continues to bear the brunt of rhino poaching in South Africa, having lost 489 rhinos so far this year.
- A total of 103 rhinos have been poached in Limpopo, 65 in KwaZulu-Natal, 47 in North West and 43 in Limpopo.
All images are copyright Philip Venter 2014, and may not be used without permission of DigiKids.


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This is one of those places that appears in a search for a hotel in Arrens-Marsous but is in fact a 40 minute walk from it. This may be okay if you're in a car but when you're walking with a backpack and its 30...More
Getting you more information on this room
Getting you more information on this room
"higher floors at the front would be better, as you have a view. Rooms at the back have the view of a wall."


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By Tim Reed, this site CEO of MYOB, viagra dosage Australia's Largest Provider of Business Management Solutions.
If you're trying to grow your business right now, then right now is the time for you to review how your business works, and how you're looking to attract new customers.
In an increasingly competitive market place, you need to give your customers a reason to visit your store – whether it's online or bricks and mortar. As a small business, you don't have the luxury of a massive marketing and promotional spend. So you need to be clever in how you attract and retain your customers.
In November, MYOB partnered with 'Shop Small' – a widespread social movement to promote local small businesses. So, now is an opportune time to review where you are now and where you want to be in the future.
Your specialty
Understand what it is that makes your business special. What is your point of difference? Why should customers choose to spend their money with your business, when there are so many other choices? The more the business is about serving others and the less it is about you and your needs (easier said than done!), the better it will work.
Understand your customer
Create a mental image of a customer you would like to serve and use this image when you are creating marketing campaigns. Who are they? Where are they? What are their needs and motivations? How do they like to be communicated with? Try to connect with them in the way that best suits them and make sure that you direct your message to them – not just to the general population.
Attract them
Depending on who your customer is, some methods will work better than others. Once you have a concrete mental image you will better be able to identify where they can be found and at what time. The better you can focus and direct your marketing efforts to those people most likely to actually pay for what you do, the better it will work.
Reward referrals
A satisfied customer willingly spreads word-of-mouth promotion. One successful tactic for many businesses is starting a referral program for existing customers to bring in new customers to your business. Offer both the existing and new customer an incentive, such as a discount or gift, for being part of the referral program. Assess the ROI and you could very well be surprised.
Use social media websites to promote sales and giveaways, advertise new customer incentives and ask existing positive customers to discuss their satisfaction with your company publicly online. Take the time to connect with other businesses in your community and industry. Stay active. Keep your eyes and ears open and join the conversation when relevant. Online promotion doesn't need to be expensive; there are numerous social media platforms available at your fingertips.
Emphasise information
An essential element of any effective marketing campaign geared to long-term customers is a focus on information they need, presented in an easily digestible way that appeals to their desires – not an over-the-top sales spiel. Before anyone buys from you, they have to trust you. The first step to establish trust is to let people know that you know what you're talking about.
Customer experience
Illustrate publicly how valuable your customers are to you. This does not mean thanking them 10 times or telling them how much you appreciate them – it means making it very easy for them to do business with you in every way you possibly can.


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ACICS – Accredited College in Florida
Florida Career College - Lauderdale Lakes holds institutional accreditation from the Accrediting Council for Independent Colleges and Schools (ACICS).
Gainful Employment Disclosures
Interested in taking your career to the next level? Contact us today and jump start the career of your dreams*. After applying, here's what you can look forward to:
Conveniently located right outside downtown Fort Lauderdale, you'll find our 50,000 square-foot facility in the Penn Dutch shopping plaza on the southeast corner of State Road 7 and Sample Road. With an ideal location that's close to nearby Florida cities, Lauderdale Lakes offers plenty of opportunities for students during their education and after graduation. During your time at our Lauderdale Lakes location, you'll have access to great technology, compassionate teachers, and a Career Services Center that's dedicated to guiding you through the process.
Florida Career College offers a variety of vocational and trade school programs that give you the skills you need to thrive in the professional world. At our Lauderdale Lakes campus, we offer the following programs:
3383 North State Road 7, Lauderdale Lakes, 33319
Tel: (754) 900-6800
At Florida Career College, our mission is to successfully prepare you for new career opportunities after you graduate. No matter where you are in life, our vocational and trade school programs will give you the tools you need to put yourself in a position to succeed after graduation. Depending on which program you select, you could go from enrolling in classes to interviewing for entry-level positions in less than a year.
Fill out the form above if you have any more questions or you're ready to enroll in a vocational or trade school program at our Lauderdale Lakes campus. We're happy to do whatever we can to help you find the right program and get you on the path to a great career. To connect with us even faster, call us right now at 888-852-7272!
Florida doesn't have to just be a place for vacation—it's home to numerous colleges and universities, as well as technical and vocational schools. As one of the leading vocational schools in Florida, Florida Career College offers undergraduate degree programs in some of today's fastest growing career fields.
Located in thriving metropolitan areas throughout the state of Florida, including Orlando, West Palm Beach, and Tampa, the education of your dreams is within reach- no matter what city you call "home". Plus, most of our Florida vocational schools are easy to get to by car, train, or bus, so you won't have to travel very long or very far to continue your education. With our library and learning resources, real-world technology, and experienced instructors, it's no wonder we're one of the best vocational schools in Florida.
If you've been looking for the right trade school in Florida, you've come to the right place. FCC is a technical training school that specializes in a wide range of career training programs, including nursing, business, and cosmetology. With such a variety of options, you'll receive the skills and knowledge you need to successfully launch your new career at FCC.
Want to learn more about a specific FCC technical school in Florida? Contact us for information about admissions, financial aid, or to find out what classes are offered at your campus of choice. At FCC, you'll find a supportive and welcoming academic environment with the career training solutions you need to confidently step into your next career.
For important information about the educational debt, earnings, and completion rates of students who attended this program, please see our Gainful Employment Disclosures and other consumer information at http://www.floridacareercollege.edu/student-consumer-info.
By submitting this form, I agree to the Privacy Policy and Terms of Service, available on this website, which I have read. I also agree IEC Corporation and/or its affiliates/subsidiaries/agents, including but not limited to Florida Career College and Florida Career College – Houston Campus, may contact me via email and/or telephone, at any phone number I have provided, including via text, automatic telephone dialing system, and/or artificial or prerecorded voice, for any purpose, including marketing. I understand that I do not need to provide this consent as a condition to receive any good or service.


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Strawberry Iced Coffee
READY IN: 5mins
Recipe by AmandaInOz
Fruity refreshing coffee drink!
Top Review by Domestic Goddess
I so wanted to give this recipe a good review, but I'm sorry to say I could not. I have made this numerous times, in hopes I'd get to like it; especially after viewing the picture of the iced coffee which Baby Kato took, but each time I tried it, I could not get past the strong coffee taste. For some reason I felt the combination of ingredients did not go good together (taste-wise).
See Discussion:2 Reviews
Ingredients Nutrition
- 1 scoop vanilla ice cream
- 1 scoop strawberry ice cream
- 1⁄2 cup cold strong coffee
- whipped cream
- 3⁄4 ounce strawberry syrup
- 2 small strawberries
Directions See How It's Made
- Put ice creams in a rocks glass.
- Pour in the coffee.
- Spoon whipped cream on top.
- Pour syrup over cream.
- Garnish with the strawberries.


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We have many visitors on this website from the Washington state area. In this post, I have included some alcohol treatment centers in the Seattle area to help you if you are undergoing any type of alcohol dependence problem.
In future posts, I will be covering different cities and areas around the world, as unfortunately excessive alcohol drinking is a global and growing problem.
Alcohol Help in Seattle and Washington State
The first thing you can do is to call the Washington Recovery Helpline, a free service to help people dealing with different kinds of substance abuse problems. Call them on 1-866-789-1511 (toll-free), 24 hours a day, 365 days a year.
There is also a tremendous resource provided by the state government called the Directory of Certified Chemical Dependency Services in Washington State.
On this page, you will see many sections to download. You will need the Adobe Acrobat PDF reader in order to be able to read these sections. You can download this free if needed. The document provides a list of helplines, inpatient and outpatient treatment centers and other types of dependence centers, all certified by the Division of Behavioral Health and Recovery (DBHR).
They also contain resources for specific groups of people who might have specialized needs such as pregnant women or young people.
University Of Washington Alcohol and Drug Abuse Institute
Another excellent resource is the University of Washington Alcohol and Drug Abuse Institute.
Their web page lists a number of major detoxification and other centers in Seattle that deal with alcoholics and alcohol dependent people in Washington State. You can find more information and the links to each of the resources very quickly. They also have many resources on academic and research on alcohol dependence as well as links to national and international government agencies.
You can use our Treatment Directory to locate a treatment center near you.
Tips and tools to help you quit alcohol or moderate your drinking right now!


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Alluring VixensHot Sites
Alluring? Definitely. This popular site features really hot girls posing in sexy outfits, bikinis and lingerie. Some get naked and some are just there to tease so it's a nice mix of content for fans of softcore and stripping. The picture sets on a AlluringVixens.com are all shot in a glossy glamor style and come with videos from the shoots. The models? Some of the hottest go-go dancers, strippers and solo girls around. Christine Mendoza, Justene Jaro, Aaliyah Love and Brianna Lee are among the many fully qualified babes that appear. Hot, tan girls with big tits and tight bodies looking their best.


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|Kaoru Mitsuki
|Kaoru Mitsuki
|June 6, 1983
|22 (First Series)
Kaoru Mitsuki (御月 カオル Mitsuki Kaoru) is the female protagonist of the series, an aspiring artist who's always having trouble making money. Though learning of the Makai Knights and the Horrors, Kaoru learns that her destiny was intertwined in the conflict since her childhood. Kaoru is portrayed by Mika Hijii (肘井 美佳 Hijii Mika). As a child, she is portrayed by Kanami Katchi (甲地 夏波 Katchi Kanami).
Born to her parents, the famous artist Yuji Mitsuki (御月 由児 Mitsuki Yūji) and Karin Mitsuki (御月 かりん Mitsuki Karin), Kaoru was six years old when she is found by Barago who converted her into a gate for his plan to bring Messiah into the world.
Kaoru gets a break the day she is able to present her art at the gallery of Tasuke Taniyama, unaware that he is possessed by the Horror Anglay who intended to devour her before Kouga arrived and bought her painting to have her take it to his manor while he attempts to slay the Horror. However, returning to the gallery as the fight reaches its climax, Kaoru is splashed with Anglay's blood with Kouga sparing her life to find a way to save her life while giving Zaruba and the Eastern Watchdogs the notion that he intends to use her as Horror bait while keeping the secret of her condition from her.
Kaoru eventually was evicted from her apartment and temporarily freeloaded at Asami's place. After Kaoru's bad cooking gave Asami food poisoning, she moved into Kouga's home. She literally took Kouga's words of staying close as an excuse to move in. Kouga wanted her to at least earn her keep, but her cooking was so horrible that Gonza became ill.
Kaoru has feelings for Kouga as well, but was devastated when she found out Kouga used her as Horror bait, even though Kouga actually never did it until the occasion where she found it out. Kaoru always seem to manage to involve herself with a Horror all on her own, and that's why Kouga always says she's an unlucky woman. For her protection, Kouga placed a special ring created by Zaruba in one of her fingers, allowing him to always locate Kaoru's position when necessary and be alerted if a Horror is close to her, though she forced Zaruba to take it back when the truth came out. Eventually, Kouga managed to save Kaoru's life with the Barankas fruit.
Kaoru also learns of his father's ties to Kouga's father Taiga, finding the picture book from her dreams that her father wrote let never finished the final age. As Barago's scheme is nearly complete, Kaoru's nature as Messiah's gate begins to manifest as he kidnaps her. Trusting her to Elda, Barago conveys to Kaoru his life story and the fact that she has also been possessed by Messiah herself at times during their meetings. Rendered in a comatose state as Messiah begins to enter the human world, Kaoru played a role in having Kouga defeat the Horror and bring him back to their world. Soon after, Kaoru presents Kouga the finished the last page of the Golden Knight and the Black Flame book before she leaves for Italy.
- Mika Hijii also portrayed Miyuki Yoshinaga/Orchid Undead from Kamen rider Blade.


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Matt Lee (mattl)'s status on Friday, 23-Jun-2017 22:02:41 UTC
Matt Lee started following Addy Lerer 🌹.
mattl.io is a social network, courtesy of Matt Lee. It runs on GNU social, version 1.2.0-beta4, available under the GNU Affero General Public License.
All mattl.io content and data are available under the Creative Commons Attribution 3.0 license.


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YA Romance Fantasy Books
12 Book Series That Are Equal Parts Sexy and Sci-Fi
Photo 6 of 12
The Maze Runner
If you throw 50 or 60 guys into a maze with no explanation and take away their memories (save their names), then clearly some crazy things will go down. Add vicious robot monsters. Now top it off with the appearance of one beautiful girl. In James Dashner's The Maze Runner, Thomas and Theresa, with their inexplicable telepathic link and vague memory of each other from a past life, must work to solve the maze if they ever want to escape into the real world and find out why they were put there to begin with. The first installment in the series has been adapted to film and is coming out this Fall, so make sure to read the books before the movie!
0previous images
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Dear Fawn
Every year the quickest runner is entrusted with being the connection between the light and dark glade. Our young friend, equipped with four legs, wings and a quick-witted talent for making wise decisions, travels at the speed of light on the long and ever-changing path between the two gates.
The Poster measures 40cm x 50cm and is printed on 170 g Munken Polar Rough matte paper to maintain the nice feel of the original in watercolor.
Limited edition, when it's sold out, it is sold out forever.


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Added by Dani Perry
Peter Estin hut has it all! After a strong hike in, it opens up to amazing views and great skiing for the whole crew to enjoy.
If you have stayed in other 10th Mountain Division Huts, Peter Estin is a quite similar and definitely one of my favorites. I made the trip in the winter because of the awesome skiing/snowboarding just right out side the front door.
The trailhead begins at Yeoman Park and there are two routes that you can take. A more scenic, flatter route is about 7 miles. Or, the one I like starts by following the road to Fulford Cave and winds up the forest. You climb about 2,000 feet in 3 miles, so make sure to leave early enough, especially in the winter, depending on your skill level.
But it is so rewarding! We spent the next day hiking around the backside of the hut to explore the epic panoramic view and ski around in the trees. If you hike to the top, which isn't too difficult, you are exposed to the most incredible views. It also links well with another hut, Polar Star.
Photo Credit: Mason Lacy.
Pack List
- Snowshoes or skins for climbing
- Sleeping bag
- Food for cooking at the hut (comes equipped with fire stove and some propane fueled stove tops and lots of cooking utensils)
- Goggles or sunglasses
- Avalanche beacon if you plan on skiing
ReviewsLeave a Review
over 1 year ago
Hey Ian! You don't really rent out the entire hut, you more of rent out a bed/space in the hut. They sleep usually 10-15 people and there are times when the whole hut will be filled with fellow hut trippers or sometimes your group will have it to themselves. It depends on the time of year for availability usually. Here is a link to the website that shows you availability and how to make reservations. Hope that helped! https://olb.huts.org
Ski Hut Question
Hey Dani! This looks amazing! What do you have to do in order to reserve a hut? Are they usually booked out in advance?
Added by Dani PerryFollow
Snowboarding, Chillin, Skiing, Snowshoeing
Skill Level:
Trail Type:
5 Miles
Elev. Gain:
2000 Feet
Are we missing something?Suggest an edit
More Adventures Nearby
Hike the Long House Trail
Colorado / Long House Trailhead
This is the only dwelling in Mesa Verde that you can access without a guided tour or a ranger. Photos: Rene Rivers and Ken Lund
Hike the Cliff Palace Loop Trail
Colorado / Cliff Palace Loop Trailhead
Tickets for this tour are $4 per person and are available at the visitor center near the entrance of the park. This tour is best taken from April to November.


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Sq. Feet
Year Built
Days On Site
24 Photos
Home Description: 15549 Pebble Ridge St
One word says it all when you walk into this gorgeous 4br, 2ba golf front home in popular Stoneybrook West. That word is W0W!! Located on the 8th hole, this home has been meticulously maintained & upgraded. Starting with the kitchen, featuring stainless steel appliances, custom-trimmed 42" solid cherry cabinets, granite countertops and Travertine tile. The formal living & dining rooms feature Lavella Taun solid-wood flooring. The family room features a gas-fireplace, solid hardwood flooring & stunning window treatments. The enormous Master Suite features Travertine Flooring, gigantic walk-in closet, dual-vanities, garden tub & separate walk-in shower. Additional features include: Newer A/C System, 6? Crown & 5? Victorian Base throughout home, home-surveillance system w/ remote access & DVR, Newer Craftsman wi-fi garage door opener. Stoneybrook West is the only 24-hr manned, security-guarded & gated, golf-course community in Winter Garden. Fantastic location! Minutes from thriving downtown Plant Street district w/ shopping, dining and cultural options, as well as nearby Winter Garden Village at Fowler Groves. Easy access to all major area roadways & attractions.
Available now at $364,900
Approximate Room Sizes / Descriptions
The average asking price of a 4 bedroom Winter Garden home in this zip code is
$457,935 (25.5% more than this home).
This home is priced at $142/sqft,
which is 9.5% less than similar homes
in the 34787 zip code.
Map |
Street |
Birds Eye
Birds Eye
15549 Pebble Ridge St Winter Garden FL 34787
Description: 15549 Pebble Ridge St
Driving Directions:
From SR 535, take Stoneybrook West Parkway to the main entrance on the right. Once through gate, proceed to the second stop sign & make left onto Tour Pointe Blvd. At second stop sign, turn left into Pebble Ridge. Follow Pebble Ridge Dr for .3 miles.
Similar to: 15549 Pebble Ridge St
For Sale Near: 15549 Pebble Ridge St
Area Stats
These statistics are updated daily from the My Florida Regional MLS. For further analysis or
to obtain statistics not shown below please call EasyStreet Realty at
(407) 278-2057 and speak with one of our real estate consultants.
Listing Courtesy of Re/Max Prime Properties.
For information or to schedule a viewing of this property (MLS# O5523896) located at 15549 Pebble Ridge St Winter Garden FL 34787, call: 407-278-2057
15549 Pebble Ridge St, Winter Garden Florida is a single family home of 2565 sqft and
is currently priced at $364,900
This single family home has 4 bedrooms and was built in 2002.
A comparable home for sale at 14880 Ellingsworth Ln in Winter Garden is listed at $377,559.
This home on 15549 Pebble Ridge St has not had a price change.
In addition to single family homes, EasyStreet also makes it easy to find Condos, New Homes and Foreclosures
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text: Kindertage Kindertage Flute, viola, electric organ, piano, accordion, zither, guitar and percussion (5 players)Chamber music No. 3. Composed by Wilhelm Killmayer. This edition: ED 6473. Contemporary Music. Performance score. Composed 1973. 16 pages. Duration 7 minutes. Published by Schott Music (SD.49006343). Usually leaves our warehouse: Within 4 to 6 weeks More info Add to Cart { "catalogRefId":"16469428", "productId":"prod16469428", "quantity":"1" }


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Vistaprint (with an expected delivery date of the 25th) arrived two days ago, and they are brilliant. One hundred cards - with the full-colour book cover image one one side and blurb with two QR codes (one for the novel on Kindle and the other for Kobo) on the reverse - came to just over £30. Which included delivery that came a week earlier than expected.
The postcards are one of the aides I'm planning to help me promote the novel during my time at the Writers' and Artists' Yearbook organised Self-publishing in the Digital Age one day conference on the 3rd November.
There are many tools at a writer's disposal in the field of self-promotion and maybe the postcard is a bit clunky, the fact that it's tactile and has the QR codes on can make it a very useful asset. We'll see but so far it's looking good.


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The Building of Maxwelltown West ChurchMay 23, 2013
The Laurieknowe area in 1850 was very different from now.
A new Free Church was to be commissioned and a design prepared by Mr. Barbour, architect, was selected and a site was obtained.
By the 1893 map, the area had changed considerably. The following report on the building of the church is taken from the book 'Jubilee 1866 – 1916 Maxwelltown United Free Church' printed for the Deacons' Court.
"The whole work, exclusive of painting, was contracted for by Messrs Crackston & Sons, builders, Maxwelltown. The foundation stone was laid by Mr. A. Murray Dunlop, M.P. on the 6th of July, 1865; and the church thoroughly completed has just been publicly opened for the worship of God.
To Mr. Costin, painter, was entrusted the pictorial ornamentation of the interior, a task he has performed with admirable skill and taste. The building appears to be a model piece of masonry, reflecting the utmost credit on Messrs Crackston & Son.
We ought not to neglect to mention that the fine toned bell of the church was the gift of Mr. J. M. Henderson, manufacturer, and that the Baillie Tweddle gave, as his subscription, £20 for purchasing the heating apparatus. The contract price for the work of the mason, joiner, slater, plasterer, etc., was £1,888; the charge for painting, heating, grates, rails, and architect's fee will be about £220; the price of the site was £165 – in all £2,273. This will leave a considerable margin of debt – probably £400 or more; but whilst thankfully acknowledging the liberality manifested by the people at the opening services, by the handsome collection of £56/14s, we trust that the collection to be taken at the services in the church tomorrow will also be on a large and liberal scale.
The church was opened by the Rev. Dr. Begg on Thursday, 15th November, at twelve o'clock. Though the day was wet and boisterous, nearly every seat was occupied, the country members of the congregation, no less than those of the town, turning out well."


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Your Why Leads The Way I'm very big on goal setting and your "Why" is just as impotant. Fitness, nutrition, travel, self-growth, life achievements, chores, daily tasks.. Whatever my mind comes up with and desires I set goals and strive to achieve it. Most of the time I get there, sometimes not as easily as […]Read More
8 Nutrition myths DEBUNKED!! Hey guys here are 8 popular nutrition myths/misconceptions broken down and explained. Carbs are the enemy and stay away at all costs. • Truth: carbs are necessary in any diet. They are what gives our muscles and body energy. Carbs yield half the calories in fat so we need to eat […]Read More
Cravings are one out of the many habits that us normal people usually have a problem kicking. Studies tell us that it's fairly common for food cravings to happen at certain times, quite often at around bedtime. Your guard may be down, you may have had an unusually hard day, and off you go on […]Read More
Introducing Fit In 42 Park Slope (New Program)
We are beyond excited to share the amazing news with all of you! Body Unique has been selected to represent Fit in 42 Park Slope Brooklyn! We are the only facility in the area offering this unique transformational program. WHAT CAN YOU EXPECT FROM FIT IN 42? An innovative program that not only includes interval […]Read More
Holiday Hold'Em 21 Day Fitness Challenge
Holiday Hold'Em 21 Day Fitness Challenge will keep weight gain at bay – No Matter How Many Holiday Get-Togethers You Attend! Are you tired of having to make the same old resolution every New Year's Day? You know, "I resolve to lose all the weight I gained at those Thanksgiving and Christmas parties". If so, […]Read More
Maintain…. Don't Gain This Holiday Season. Tips For You To Be Successful, Healthy and Happy!
We want you to be able to maintain this holiday season, not gain! There will be parties, celebrations, food and champagne.Ahhhh….that irresistible succulent Thanksgiving turkey and mashed potatoes . The endless desserts. That mouthwatering New Year buffet and the countdown champagne are so tempting. Its instantly satisfying!So you binge.So what is the price you pay […]Read More
Injury prevention is a common term not just in gyms, but everyday life as well. In general we tend to misuse the term. By that I mean we tend to seek treatment after the fact. That is injury rehabilitation. Prevention is the action of stopping something from happening or arising. So before stepping into the […]Read More


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The Greedy Town is the nineteenth episode of the fourth season of Rawhide. It first aired on February 16, 1962.
Clad in deepest mourning, a very wealthy woman comes to the small town where her dead son robbed a bank and killed a man. She is prepared to pay each resident several years' income if they agree to wipe her son's crimes off the books. - Source: IMDb.com
- Eric Fleming as Gil Favor - credit only
- Clint Eastwood as Rowdy Yates
- Paul Brinegar as Wishbone
- Charles Gray as Clay Forrester
Special Guest StarEdit
- James Murdock as Mushy - credit only
- Steve Raines as Jim Quince
- Rocky Shahan as Joe Scarlet
- Robert Cabal as Hey Soos
Also StarringEdit
Supporting castEdit
- J. Pat O'Malley as George Emory
- Kathleen Freeman as Mrs. Beamish
- Ross Elliott as Bix Thompson
- William Phipps as Floyd Peters
- Dean Fredericks as Jed Harvey
- Addison Richards as Judge Wainwright
- Roy E. Glenn, Sr. as Joshua
- Fred Lerner as Lon
- Chuck Hicks as Billy
- Jim Galante as Handyman
Crew (and other credits)Edit
Opening CreditsEdit
- Produced by: Endre Bohem
- Teleplay by: Tom Seller
- Story by: Lew Lantz
- Directed by: Murray Golden
Closing CreditsEdit
- Rawhide Theme Created and Composed by: Dimitri Tiomkin
- Lyrics by: Ned Washington
- Recorded by: Frankie Laine
- Associate Producer: Mel Epstein
- Story Consultant: Lou Morheim
- Production Manager: Harry Templeton
- Director of Photography: Jack Swain
- Art Directors: John B. Goodman and George W. Davis
- Set Decoration: Budd S. Friend
- Assistant Director: Kurt Neumann, Jr.
- Film Editor: James Baiotto
- Music Supervision: Leon Klatzkin
- Casting: Russel Trost
- Recording Supervisor: Franklin Milton
- Sound Mixer: Fred D. Faust
- Titles by: Pacific Title
"Filmed at Metro-Goldwyn-Mayer Studios | A CBS Television Network production"


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News & Updates
Do You Have an Idea for a New Workshop at PAG XXVI?
All professionals in the Plant and Animal Genome community are welcome to submit a Workshop Proposal.
Proposals should be initiated with the intent of fostering the development of genomic analysis of agricultural products to improve the world food supply. We are interested in novel sessions which focus on, but are not limited to crop, livestock, aquaculture and model species genomics.
Once submitted, the proposal will be sent to the Organizing Committee for review. Please begin the process by filling out a Workshop Proposal Submission Form, and if accepted, we will contact you to discuss further.
Note, all submissions MUST include a list of proposed speakers
** Deadline for proposals is September 15, 2017 **


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Lincoln Logs Forge Mill Cabin Building Set
This is a Lincoln Logs Forge Mill Cabin Building Set that's produced by the good folks over at K'Nex.
They make some of the neatest building sets on the market today.
The Lincoln Logs Forge Mill Cabin Building Set contains 51 pieces and is made of real wood.
Recommended Age: 3+
Condition: Brand New
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- K'Nex Starter Rocket Car Building Set $9.99
- K'Nex Starter Stealth Plane Building Set $9.99


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Hi all, I got around to writing the report I always needed: how much time each job actually runs and how much time it spends waiting in the SGE queue.
The attached shell script produces the report, combining information from SGE's QACCT with the galaxy job/dataset information. The output contains: job, user, tool name, dbkey, total input size in bytes, waiting time in SGE queue, actual SGE executiong running time, and some other tidbits. This allows finding how much running time each user had on the cluster, how much time each tool/user spend idly waiting, and some possible correlations between tools, dbkeys, input size and running time. The script is tightly coupled with SGE and PostgreSQL, but can probably be adapted to PBS/MySQL. Hope this helps someone, -gordon
Description: collect_sge_job_timings.sh
___________________________________________________________ Please keep all replies on the list by using "reply all" in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http://lists.bx.psu.edu/


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Fensterbild Ostern Hasenwiese
Victorian Gable Ornament dollhouse inspiration Love it!
A Wooden Case That Turns Your iPad Mini Into A 1950s Television Set - DesignTAXI.com
Suzanne Dege's "Hobbit Treehouse." Originally built by the legendary natural builder, SunRay Kelley. Located on Orcas Island in Washington State.
Not great picture quality, but the backlight emphasizes the fantastically intricate railings and spandrels.
Pergola with fireplace/oven
Arboria Trellis Column - 72"
Arboria Trellis Column - 72"
Tool Tote Sawhorses - I'm often looking for a place to set tools when working on the sawhorses so this is an interesting idea. Sacrifices the option to have collapsible sawhorses, though.
Plans to DIY a nice folding sawhorse with a shelf. It can be a horse, table legs, armour rack and more. This will work great for LARPing/camping and it's easy to fold down for traveling. -


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In 2014, the Limpopo districts of Waterberg and Mopani were badly affected by severe storms and gales that destroyed and damaged houses in a number of villages, rendering households destitute.
The KwaZulu-Natal master spatial plan (MSP) for human settlements was finalised in April.
The community of Knysna was overjoyed when the Minister of Human Settlements, Lindiwe Sisulu signed the transfer of 72 ha of land to the HDA for the development of integrated human settlements.
HDA hosted sector workshop on unauthorised land occupation and its impact on the upgrading of informal settlements on 17 May 2016
The dysfunctional procurement system used in the Nelson Mandela Bay Municipality was among the HDA's areas of concern when appointed to support the metro's human settlements delivery programme in 2015.


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The guanidinium alkaloids tetrodotoxin (TTX) and saxitoxin (STX) are classic ligands of voltage-gated sodium channels (VGSCs). Like TTX and STX, μ-conotoxin peptides are pore blockers but with greater VGSC subtype selectivity. μ-Conotoxin KIIIA blocks the neuronal subtype NaV1.2 with nanomolar affinity and we recently discovered that KIIIA and its mutant with one fewer positive charge, KIIIA[K7A], could act synergistically with TTX in a ternary peptide·TTX·NaV complex. In the complex, the peptide appeared to trap TTX in its normal binding site such that TTX could not readily dissociate from the channel until the peptide had done so; in turn, the presence of TTX accelerated the rate at which peptide dissociated from the channel. In the present study we examined the inhibition of NaV1.2, exogenously expressed in Xenopus oocytes, by STX (a divalent cation) and its sulfated congener GTX2/3 (with a net +1 charge). Each could form a ternary complex with KIIIA and NaV1.2, as previously found with TTX (a monovalent cation), but only when STX or GTX2/3 was added before KIIIA. The KIIIA·alkaloid·NaV complex was considerably less stable with STX than with either GTX2/3 or TTX. In contrast, ternary KIIIA[K7A]·alkaloid·NaV complexes could be formed with either order of ligand addition and were about equally stable with STX, GTX2/3, or TTX. The most parsimonious interpretation of the overall results is that the alkaloid and peptide are closely apposed in the ternary complex. The demonstration that two interacting ligands ("syntoxins") occupy adjacent sites raises the possibility of evolving a much more sophisticated neuropharmacology of VGSCs.
Voltage-gated sodium channels (VGSCs) are responsible for the upstroke of action potentials in excitable cells. Tetrodotoxin (TTX) and saxitoxin (STX) are guanidinium alkaloids (Fig. 1A) that block six of the nine isoforms of the pore-bearing α-subunit of mammalian VGSCs with Kd values in the nanomolar range (Catterall et al. 2005). μ-Conotoxins, which also block VGSCs, are peptides of 16 to 25 residues, including six cysteines that form a characteristic disulfide framework (see Fig. 1B) (Terlau and Olivera 2004). In contrast to the promiscuity of TTX and STX, GIIIA, the best-studied μ-conotoxin, is significantly more potent against the skeletal muscle subtype of channel NaV1.4 (half-maximal inhibitory concentration [IC50] = 19 nM), than other TTX-sensitive subtypes, such as brain NaV1.2 (IC50 ≈ 2.7 μM) (Safo et al. 2000). TTX, STX, and GIIIA competitively bind to site 1 of NaV1.4, located at the outer vestibule of the channel's pore (Al-Sabi et al. 2006; Cestele and Catterall 2000; French and Terlau 2004; Moczydlowski et al. 1986).
We recently discovered μ-conotoxins KIIIA and SIIIA, which block NaV1.2 with higher affinity than NaV1.4 (Bulaj et al. 2005; Yao et al. 2008; Zhang et al. 2007). Single-channel analyses of brain VGSCs in planar lipid bilayers showed that KIIIA produced only a partial block (Zhang et al. 2007). In saturating concentrations of KIIIA or its mutant KIIIA[K7A], a residual sodium current (rINa) could be macroscopically recorded in voltage-clamped Xenopus oocytes expressing NaV1.2 (Zhang et al. 2009). Remarkably, TTX blocked the rINa with either peptide, albeit 102- to 104-fold more slowly than control INa. When TTX was bound to the channel first, only a modest nearly 25% decrease in the rate of peptide binding was observed. The rates of recovery from block of the ternary complexes (koff values) were the same regardless of the order of ligand addition. The koff values for the bimolecular complex KIIIA[K7A]·NaV and the ternary complexes KIIIA·TTX·NaV and KIIIA[K7A]·TTX·NaV were similar; in contrast, the koff value for KIIIA·NaV was 10-fold smaller, indicating that the presence of TTX decreased the stability of KIIIA·TTX·NaV. We hypothesized that TTX and peptide bound abutting microsites (Olivera et al. 1991), within the greater confines of a macrosite synonymous with neurotoxin receptor site 1 designated by Catterall (Catterall 1980; Cestele and Catterall 2000).
In this report we tested STX, which has two positive charges (Bordner et al. 1975; Schantz et al. 1975), in contrast to TTX's single charge (Hille 1975), and GTX2/3, a naturally occurring STX congener with a net +1 charge (Fig. 1A). In view of their rather different structures, it is not surprising that STX and TTX do not bind in an identical fashion to VGSCs. For example, when Phe 385 near the selectivity filter of NaV1.2 is mutated to Cys, the channel's affinity for TTX is reduced 3,000-fold (Zhang et al. 2009), whereas that for STX is reduced (only) 340-fold (Heinemann et al. 1992). Herein, we show that STX and GTX2/3 behave differently from each other as well as from TTX in the formation and dissociation of complexes of peptide and alkaloid with NaV1.2. Nevertheless, the overall results are explicable in terms of the reaction scheme in Fig. 1C and support the hypothesis that in the ternary peptide·alkaloid·NaV complex, KIIIA or KIIIA[K7A] cooccupies site 1 with alkaloid, irrespective of whether it is STX, GTX2/3, or TTX.
Oocyte electrophysiology
Oocytes expressing rat NaV1.2 were prepared and two-electrode voltage clamped essentially as previously described (Fiedler et al. 2008; Zhang et al. 2007). Oocytes were harvested from Xenopus frogs, following a protocol approved by the University of Utah's Institutional Animal Care and Use Committee that conforms to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Briefly, oocytes were bathed in ND96 and sodium currents (INa) were recorded by holding the membrane potential at −80 mV and stepping to −10 mV for 50 ms every 20 s. The oocytes were exposed to toxin in a small (30 μl) static bath to conserve material. Recordings were done at room temperature (∼21°C).
μ-Conopeptides were synthesized as previously described (Zhang et al. 2007). TTX was obtained from Alomone Labs (Jerusalem, Israel), STX from either Calbiochem (San Diego, CA) or the National Research Council Canada (Halifax, Nova Scotia, Canada), and GTX2/3 from the National Research Council Canada. In experiments involving high concentrations, STX and GTX2/3 were lyophilized then dissolved in ND96. All stocks were stored at −20°C in a nonfrost-free freezer until use.
Data analysis
Off-rate constants (koff values) were determined from single-exponential fits of peak INa following toxin washout; observed rate constants (kobs values) were obtained by single exponential fits of INa following toxin addition and on-rate constants (kon values) were obtained from slopes of kobs versus [toxin], all as reported previously (West et al. 2002). In particular instances (see the text immediately following), the time course of the recovery of INa following toxin washout was biphasic and the recovery was fit to a double-exponential curve as previously reported (Zhang et al. 2009).
Numerical simulation of the reaction scheme in Fig. 1C
To model the time-dependent changes in the concentrations of the various components, the reaction scheme in Fig. 1C was simulated by reiteration of the following formulas in a for-loop using the graphical programming language LabVIEW (National Instruments, Austin, TX) Pseudofirst-order kinetics was assumed; i.e., the concentration of alkaloid, denoted by [$] (representing STX, GTX2/3, or TTX), and that of μ-conopeptide, denoted by [P], remained constant. When concentrations were expressed in micromoles (μM) and time in minutes, a time increment Δt of 10−4 proved sufficiently small to accommodate the concentrations and rate constants used for the simulations used in this study. The rate constants are as denoted in Fig. 1C and their values were obtained by measuring the kinetics of block and recovery of INa following exposure to, and washout of, alkaloid and peptide, each alone or in combinations, as follows: kRA and kAR were the kon and koff values, respectively, of the reaction of NaV with peptide alone; kBR and kRB were the kon and koff values, respectively, of the reaction of NaV with alkaloid alone; and kAC was determined from the kon value for the block of residual current of the peptide·NaV complex at saturating [peptide]. There was no direct means to determine kCA, but its value could be calculated, since the product of the rate constants for the four clockwise steps equals that for the four counterclockwise steps; that is, kCA = [kRA*kAC*kCB*kBR]/[kRB*kBC*kAR]. When all of the NaV was converted into a ternary complex by use of saturating concentrations of toxins, the time course for functional recovery after toxin washout could be fit by a single-exponential curve with a rate constant koff, which was smaller than kBR (and larger than kAR), indicating that kCB was rate-liming during toxin washout; therefore kCB could be approximated by koff. A more accurate value of kCB could be estimated from the experimentally determined values for six of the rate constants, kRA, kAR, kAC, kRB, kBR, and kBC (see next paragraph for experimental determination of kBC), and calculating the seventh, kCA, as described earlier, while the value of kCB was varied until the mean squared error between the simulated and experimental time courses of recovery from block reached a minimum. Values of kCA and kCB determined in this fashion are presented in Table 4.
The value of kBC was estimated from experiments in which alkaloid was added before peptide and exploiting the large disparity between kCB and kBR (except in one instance, involving KIIIA and STX; see following paragraph), which produced a double-exponential time course for the recovery of INa following toxin washout. The coefficients of the double-exponential fits provided the koff values and spans of the fast and slow phases. In every case, the fast koff had a value close (within a factor of ∼2) to kBR (i.e., the rate constant for the recovery from block of the binary alkaloid·NaV complex) and the slow koff had a value close (within a factor of ∼2) to kCB (i.e., the rate constant for recovery from block of the ternary complex formed with saturating concentrations of both alkaoid and peptide applied for sufficient time to convert all of the NaV into a ternary complex). These results supported the notion that the span of the slow phase was likely to provide a good estimate of the fraction of the channels that were in the ternary peptide·alkaloid·NaV complex at the start of toxin washout (Zhang et al. 2009). It was assumed that preceding toxin washout, the fraction of ternary complex formed (FTCF) as a function of time followed a single-exponential time course and therefore the estimated value for the observe rate constant kobs was calculated from the equation kobs = [ln (FTCF)]/time. For the sake of simplicity, data were obtained only at a single time point. This kobs was used with the equation kobs = kon*[peptide] + koff and, since koff (i.e., ∼kCB) was invariably about an order of magnitude smaller than kobs, the apparent kon (i.e., kBC) was calculated from kobs/[peptide].
A procedure different from that described earlier had to be used to estimate kBC in the case of STX and KIIIA because the difference between kCB and kBR was only fourfold and the experimental time course of recovery of INa following toxin washout could not be fit by a double-exponential curve better than a single-exponential one. Instead, the experiment was simulated using different values for kBC and the value producing simulated results that corresponded best with the experimental data was used to approximate kBC. The remaining rate constants used in this approximation are specified in results.
Molecular modeling and docking of NaV1.2, μ-conotoxin KIIIA, and the alkaloids TTX and STX
A model of rat NaV1.2 was constructed using as a template the model of rat NaV1.4 proposed by Lipkind and Fozzard (2000), which was composed of 12 parts from the four domains. Each domain was represented by three structural fragments: S5-segment, P-loop, and S6-segment. Sequence analysis of these 12 parts from NaV1.2 and NaV1.4 shows 91.0% identity (data not shown). We found every structural fragment for each domain of NaV1.2 that corresponded to Lipkind and Fozzard's model of NaV1.4. Our model of NaV1.2 was built by introducing modifications of specific residues using PyMOL (DeLano Scientific, Palo Alto, CA); the residues of the NaV1.4 model were replaced by corresponding backbone-dependent rotamer residues of the NaV1.2 using the PyMOL mutagenesis function. The conopeptide KIIIA was constructed with coordinates from Khoo et al. (2009) and the coordinates of TTX and STX were from Tikhonov and Zhorov (2005).
Automated docking of TTX, STX, and KIIIA to the model of NaV1.2 was performed using the AutoDock4.2 software package (Morris et al. 2009). Flexible torsions of ligands were assigned using AUTOTORS and a grid box defined by 60 × 60 × 60 grid points and spacing of 0.375 Å with AUTOGRID. Docking simulations were performed using a Lamarckian genetic algorithm (Morris et al. 1998), with the following parameters: population size = 300, mutation rate = 0.02, crossover rate = 0.8. The numbers of dockings per simulation were 50 and 256 for conopeptide and alkaloid, respectively. The number of dockings per simulation for conopeptide was lower than that for alkaloid due to the larger values of flexible torsions and corresponding longer simulation times. The maximum number of energy evaluations was set to 250,000 and the maximum number of generations was 27,000. One cluster-representative structure was selected from each of the docking runs. Clusters were examined for quantity of conformations and those with the greatest number were chosen for further analysis. A secondary criterion for selecting a cluster-representative structure was the value of binding energy.
Residual Na current (rINa) with KIIIA[K7A] can be blocked by both STX and GTX2/3; however, block of the rINa with KIIIA by either of these alkaloids could not be demonstrated
As we previously showed (Zhang et al. 2009), when oocytes expressing NaV1.2 were voltage clamped and exposed to saturating concentrations of either KIIIA or KIIIA[K7A], a residual sodium current (rINa) of 5 and 23% of control INa, respectively, persisted (Fig. 2A). In contrast, saturating concentrations of the alkaloids STX or GTX2/3 produced essentially a complete block (>99%), which was much more quickly reversible than the block by either conopeptide alone (Fig. 2, A and B).
The rINa with KIIIA was not blocked by either STX or GTX2/3 at the highest concentrations tested (10 μM); i.e., ≲10%, if any, of the rINa was blocked (Fig. 2C). In contrast, the rINa with KIIIA[K7A] could be readily blocked by STX and GTX2/3 (Fig. 2D). With each alkaloid, complete block was achieved with the formation of a ternary KIIIA[K7A]·alkaloid·NaV complex, as evident from the rate of recovery from block after toxin washout, which occurred in a single phase (Fig. 2D) without an initial fast phase that would indicate the recovery from block by alkaloid alone (also see following text).
The time course of the onset of block by STX and GTX2/3 of control INa and rINa with KIIIA[K7A] could be fit with single-exponential curves (Fig. 3,A–D), and the resulting observed rate constants (kobs values) were linearly dependent on the concentration of alkaloid (Fig. 3, E and F). For comparison, also illustrated in Fig. 3, E and F are previously acquired results with TTX (Zhang et al. 2009). The slopes of the lines yielded apparent kon values, which are tabulated in Table 1. The presence of KIIIA[K7A] decreased the on rates of all three alkaloids by at least two orders of magnitude.
Ternary complexes of KIIIA·alkaloid·NaV and KIIIA[K7A]·alkaloid·NaV can be formed by adding STX or GTX2/3 first, followed by peptide
Instead of adding peptide first, then alkaloid, experiments were also performed in which the order of ligand addition was reversed. Formation of a ternary complex was evident from the single-phase nature of the recovery of activity when both toxins were washed out (Fig. 2, E and F). The rates of functional recovery during dissociation of the ternary KIIIA[K7A]·alkaloid·NaV complex after toxin washout were similar for both STX and GTX2/3. This was so regardless of whether alkaloid or peptide was added first and the off rates were essentially the same as those for the recovery from block of the binary KIIIA[K7A]·NaV complex (Fig. 2, A, D, and F, Table 2).
Although we were unable to show that the rINa with KIIIA could be blocked by STX or GTX2/3, formation of a ternary KIIIA·alkaloid·NaV complex could be readily demonstrated by adding STX or GTX2/3 first, followed by KIIIA: on toxin washout, the respective rates of recovery of block were >100- and >10-fold faster than that after exposure to KIIIA alone and 6- and 20-fold slower than that after exposure to the respective alkaloid alone (Fig. 2E, Table 2).
Rates of formation of ternary complexes when peptide was added after alkaloid
In view of the dramatic reduction by the presence of peptide in the rate of binding of alkaloid to the channel, it was of interest to see how the presence of alkaloid affected the rate of binding of peptide to the channel. After exposure to a saturating concentration of alkaloid to form the fully blocked binary alkaloid·NaV complex, the amount of the ternary complex formed following addition of peptide was determined by analyzing the recovery from block following toxin washout as described in methods. For all but the KIIIA·STX·NaV complex, after exposure to both alkaloid and peptide using a protocol similar to that used in Fig. 2, E and F but with tenfold lower peptide concentration, the time course of recovery of activity following toxin washout had two distinct phases that could be fit by a double-exponential curve (Fig. 4, A and B): an early fast phase attributed to the off rate of the binary alkaloid·NaV complex and a slower phase attributed to the dissociation of the ternary peptide·alkaloid·NaV complex—these attributions were inferred because the respective koff values were within a factor of 2 of the expected values in Table 2. As elaborated in methods, the span of the slow component yields the fraction of ternary complex formed (FTCF) from which the apparent kon value for the reaction peptide + alkaloid·NaV → peptide·alkaloid·NaV, provided in Table 3, was calculated. The approach used here was a simplified version of the protocol used to determine the apparent kon of KIIIA and KIIIA[K7A] binding to the channel preoccupied with TTX, where a fixed concentration of peptide was used and the peptide exposure time was varied (Zhang et al. 2009); in those experiments the apparent kon value for the reaction peptide + TTX·NaV → peptide·TTX·NaV was 0.13 ± 0.006 and 0.20 ± 0.008 μM−1·min−1 with KIIIA and KIIIA[K7A], respectively. These values are close to the corresponding kon values obtained in the present experiments with these two peptides and TTX (Table 3) and suggest that good estimates of kon were obtained by the simpler protocol used here.
As noted previously, with KIIIA and STX, the off rate for the recovery from block of the ternary complex was only sixfold slower than that of the binary STX·NaV complex (Table 2). Consequently, when the experimental protocol used earlier was used with these ligands, the washout data were not fit by a double- any better than by a single-exponential curve, presumably in part because of experimental noise. Nevertheless, the formation of the ternary KIIIA·STX·NaV complex could be discerned from the observation that the rate constant of the fit to a single-exponential curve decreased as the concentration of KIIIA increased (Fig. 4, C and D). The reaction scheme in Fig. 1C was numerically simulated for KIIIA and STX as ligands using the algorithm described in methods. The following rate constants were used in the simulation: kRA and kAR, 0.3 μM−1·min−1 and 0.0016 min−1, respectively (Zhang et al. 2009); kRB, 254 μM−1·min−1 (Table 1); kBR, 2.0 min−1 (Table 2); kAC and kCA, both 0 (since STX was unable to block rINa with KIIIA, as described earlier); kCB, 0.35 min−1 (from the limiting koff value in Fig. 4D at high [KIIIA]); and, finally, kBC (i.e., kon for the reaction KIIIA + STX·NaV → KIIIA·STX·NaV) was varied over a tenfold range from 0.03 to 0.3 μM−1·min−1, where the latter value is that of kRA (i.e., the kon for the reaction KIIIA + NaV → KIIIA·NaV) (Zhang et al. 2009). Simulated decay curves for the recovery from block are illustrated in the inset of Fig. 4C. It is apparent by visual inspection that the pattern of the simulated curves with the kon of 0.09 μM−1·min−1 most closely resembles that of the experimental curves. On fitting each of the experimental and simulated decay curves in Fig. 4C to a single-exponential time course and plotting the resulting koff values as a function of [KIIIA], the formation of the ternary complex was reflected by the decreasing koff value as [KIIIA] increased, until a limiting value of koff was achieved at presumably saturating levels [KIIIA] (30 μM) (Fig. 4D). In the figure, the congruence between the experimental koff values (circles) and those obtained by simulation (solid line) suggests that the kon for the reaction KIIIA + STX·NaV → KIIIA·STX·NaV was about 0.09 μM−1·min−1.
Table 4 shows the equilibrium binding constants (i.e., koff/kon ratios or Kd values) for each of the four limbs of the reaction scheme in Fig. 1C. The table also indicates the predicted rate constants obtained by numerical simulation that were used, along with rate constants presented in previous tables, to derive the Kd values. It might be noted that direct determinations of steady-state IC50 values for alkaloids, corresponding to the Kd values in saturating [peptide], were not obtained because the time it would have taken to reach steady-state block of rINa, using alkaloid concentrations near the anticipated IC50 values (≈Kd values), was hours and outside the roughly 1-h time window of our experiments. Corresponding IC50 values for peptides in saturating [alkaloid] were not obtained because, of course, no sodium currents are detectable under those circumstances.
In this study, we used two μ-conotoxins and two alkaloids and tested all four peptide–alkaloid combinations on NaV1.2. The results, together with those obtained previously with TTX (Zhang et al. 2009), support the idea that bound conopeptide not only increases the access resistance to sodium ions, as evident by the diminutive rINa, but moreover also restricts the alkaloid's access to its binding site on the channel. The results also support the notion that in the ternary peptide·alkaloid·NaV complex, the peptide traps the alkaloid at the latter's binding site and the alkaloid does not dissociate from the channel until the peptide has dissociated.
Implications of the rates of functional recovery following toxin washout of ternary complexes
A ternary complex could be formed by either order of toxin addition in four peptide–alkaloid pairs: one pair with KIIIA (in which the alkaloid was TTX) and three pairs with KIIIA[K7A] (in which the alkaloid was, respectively, TTX, STX, and GTX2/3). In each instance, essentially the same koff value was obtained for a given pair (Table 2), suggesting that for each pair the same ternary complex was formed regardless of order of toxin addition. Thus all totaled, it appears that six unique ternary (peptide·alkaloid·NaV) complexes can be formed with the two peptides and three alkaloids tested thus far.
Comparison of the koff values of the six ternary peptide·alkaloid·NaV complexes indicates that by far the least stable ternary complex was KIIIA·STX·NaV (Table 2). The other ternary complexes had remarkably similar koff values, as if TTX and GTX2/3 can shield Lys7 of KIIIA from the same site(s) on the channel. The presence of alkaloid destabilized the KIIIA[K7A]·alkaloid·NaV complex minimally with TTX and hardly at all with STX and GTX2/3. Compared with TTX and GTX2/3, STX has an extra positive charge, as does KIIIA compared with KIIIA[K7A]; thus we hypothesize that charge repulsion accounts for the relatively high instability of the ternary KIIIA·STX·NaV complex.
Implications of the on rates for the formation of the ternary complex
TTX blocked the rINa with KIIIA at a rate 104-fold slower than it blocked control INa (Zhang et al. 2009) and the block of rINa by STX and GTX2/3 were not observed even at the highest concentrations tested (10 μM) (Table 1). Thus KIIIA bound to the channel severely restricted the access of TTX and that of STX and GTX2/3 even more so. In striking contrast, for the block of the channel preoccupied by KIIIA[K7A], the kon values of all three alkaloids were more modestly reduced and, furthermore, to essentially the same value (Fig. 3F, Table 1). A possible mechanism for this is that the binary peptide·NaV complex flickers between two states: a permissive state wherein an alkaloid can "sneak" by the peptide and a state in which the alkaloid cannot. The rate at which an alkaloid sneaks by would be dictated by the fraction of time the complex dwells in the permissive state and how readily the alkaloid can permeate that state. The permissive state with KIIIA may be permeable to TTX but not to STX or GTX2/3, whereas that with KIIIA[K7A] may be equally permeable to all three alkaloids. This would explain why the alkaloids have similar on rates in blocking the rINa with KIIIA[K7A], even though they block the control INa at very different rates; i.e., the rate-limiting factor for rINa block is the fractional dwell time of the permissive state, which is intrinsic to the peptide and independent of the specific alkaloid.
Examination of the rates of recovery from block allowed the apparent kon for the reaction to be estimated (Fig. 4). When alkaloid was already bound to the channel, the rate at which peptide could subsequently bind to the channel appeared to be only modestly impeded, if at all. With KIIIA[K7A] and STX, GTX2/3, or TTX (Fig. 4A) and with KIIIA and GTX2/3 or TTX (Fig. 4B), the apparent kon values were within a factor of 2 of each other (Table 3). This was consistent with previous results with KIIIA and KIIIA[K7A], where the presence of TTX on the channel was observed to reduce the apparent kon of the peptide by only about 25% by Zhang et al. (2009). STX bound to the channel reduced the apparent kon of KIIIA by about threefold, from 0.3 μM−1·min−1 under control conditions (Zhang et al. 2009) to about 0.1 μM−1·min−1 (Fig. 4, C and D).
Equilibrium constants derived for the proposed reaction scheme in Fig. 1C
The upper and lower limbs on the left side of the reaction scheme in Fig. 1C represent simply the binding of peptide and alkaloid, respectively, to the channel. The upper and lower limbs on the right represent binding of alkaloid and peptide, respectively, to the channel preoccupied with the opposite ligand; their convergence to form a single species of ternary complex for a given peptide/alkaloid pair, although hypothetical, is compatible with the results of our experiments. Table 4 lists the apparent binding constants of the reactions in all four limbs of the scheme. The Kd of TTX for the channel saturated with KIIIA was 20-fold larger than that without the peptide, where the presence of KIIIA decreased TTX's kon (i.e., block of control INa vs. rINa) about 20,000-fold (Table 1) and koff [i.e., koff of binary (Table 2) vs. ternary complex (predicted kCA, footnote Table 4)] about 1,000-fold. (Note that the SD of the predicted kCA is responsible for the large SD in Kd here.) In contrast, the presence of KIIIA[K7A] only modestly altered the Kd values of TTX, STX, and GTX—the apparent kon (Table 1) and predicted koff (kCA, Table 4) values for a given alkaloid were both decreased by two orders of magnitude or more. This raises the possibility that the binding site of the alkaloids per se may not have been strongly affected by KIIIA[K7A]; instead, access to and egress from the binding site was retarded by the presence of the peptide. This conjecture is compatible with the flicker mechanism for the binary peptide·NaV complex suggested in the preceding section.
For each ternary complex, the predicted kCB value (Table 4) was close to the measured koff value for the recovery of INa during toxin washout (Table 2), consistent with the idea that the rate-limiting step in disassembly of the ternary complex is the dissociation of peptide. Likewise, kCB was always greater than kCA except for the KIIIA[K7A]·TTX·NaV complex, in which the two rate constants were predicted to be similar (Table 4).
The Kd of KIIIA[K7A] binding was approximately the same irrespective of whether the channel was preoccupied with alkaloid, regardless of the alkaloid species; here, neither kon nor predicted koff was affected much by the presence of alkaloid (Table 4B). In stark contrast, the Kd of KIIIA was increased close to 20-, 800-, and 60-fold by TTX, STX, and GTX2/3, respectively; in each case, an increased koff value was responsible for most of the elevation in Kd (compare kCB values in Table 4 with the koff, i.e., kAR, value in Table 2).
Our overall results support the conclusion that the presence of alkaloid minimally interfered with the accessibility of peptide, whereas the presence of peptide severely occluded the accessibility of alkaloid. This striking asymmetry is consistent with the picture of the ternary complex wherein the alkaloid is sandwiched between the peptide and selectivity filter of the channel (Zhang et al. 2009). We explored the feasibility of this "sandwich" model in silico, as described in the immediately following text.
Structural models of alkaloid and peptide docked at the outer vestibule of the channel
Following the advent of the cloning of VGSCs, experiments to pinpoint the binding site for TTX and STX have been carried out by several investigators; the general consensus is that the alkaloids bind at a site deep in the outer vestibule of the channel to cork its pore (Choudhary et al. 2002, 2003, 2007; Lipkind and Fozzard 1994, 2000; Penzotti et al. 1998, 2001; Santarelli et al. 2007; Terlau et al. 1991; Tikhonov and Zhorov 2005). On the other hand, studies with GIIIA and NaV1.4 indicate that the conopeptide binds at a more superficially located site (Chang et al. 1998; Choudhary et al. 2007; Dudley Jr et al. 1995, 2000; Hui et al. 2002; Li et al. 2001; Xue et al. 2003).
Given this background work by others, it behooved us to simply see whether both KIIIA and TTX or STX would fit in the vestibule. We present here our initial attempts to examine this by docking alkaloid and peptide in silico, as described in methods. These docking experiments are based on the sodium channel model proposed by Lipkind and Fozzard (2000). The model, which consists of the S5- and S6-fragments and P-loop present in each of the four domains of the channel, was built starting with the structural framework of the KcsA potassium channel (Doyle et al. 1998). The approach of using such models to analyze the results of electrophysiological measurements of sodium channel mutants has proven instructive (see references published after 2000 cited in preceding paragraph).
Illustrated in Fig. 5 are the pore loops at the outer vestibule of NaV1.2, including the DEKA "ring" forming the ion selectivity filter (Hille 2001) and the more superficial EEDD ring of negative charges that are common to all mammalian NaVs (except NaV1.9, where the latter ring is EEED). STX and TTX were each docked before KIIIA (Fig. 5, A and B, respectively) as well as after KIIIA (Fig. 5, C and D, respectively). Indeed, both KIIIA and alkaloid can simultaneously fit in the vestibule. Furthermore, when KIIIA was docked first, TTX could also dock close to the selectivity filter (Fig. 5D); however, this was not the case with STX (Fig. 5C). Thus these results are, to a first approximation, consistent with our experimental observations. It would be interesting to examine the docking behaviors of the alkaloids when KIIIA[K7A] is docked first, as well as the docking of other peptides and alkaloids that have yet to be tested experimentally (see following text).
Aside from GTX2/3, 15 other naturally occurring congeners of STX have been identified (Yasumoto and Murata 1993). Experiments with: 1) these congeners as well as possible synthetic derivatives of both STX and TTX (good progress in guanidinium alkaloid syntheses is promising in this regard; Andresen and Du Bois 2009; Mulcahy and Du Bois 2008; Nishikawa et al. 2004; Sato et al. 2008); 2) close relatives of KIIIA, such as the μ-conotoxins SIIIA and SmIIIA (Bulaj et al. 2005; West et al. 2002); and 3) mutants of the channel, such as NaV1.2[F385C] (Zhang et al. 2009), should advance our understanding of the mechanism of action of guanidinium alkaloids and μ-conopeptides that target site 1 of VGSCs. Furthermore, if active, covalently linked alkaloid–peptide adducts can be synthesized, these should provide insights into the arrangement of the units when bound to site 1, as well as also serve as second-generation ligands for sodium channels.
We introduced the descriptor syntoxin to denote the phenomenon wherein toxins such as TTX and KIIIA can act in concert (Zhang et al. 2009). From a broader perspective, investigation of the syntoxin interactions of guanidinium alkaloids and conopeptides with VGSCs may contribute to more than just our understanding of the structure and function of sodium channels per se. For example, STX is a major active factor in paralytic shellfish poisoning, a serious public health hazard (Al-Sabi et al. 2006; Bricelj et al. 2005). KIIIA is a lead compound in the development of an antidote to STX in the form of a contratoxin; i.e., a ligand that shields the target receptor against a toxin (Zhang et al. 2009). KIIIA's ability to prevent STX binding augurs well for its development into a contratoxin, although it remains to be seen whether KIIIA can be sculpted to minimize its efficacy in interfering with sodium conductance (that is, engineered to increase its rINa) without excessively compromising both its affinity for the channel and its ability to interfere with STX binding. It also remains to be shown how conserved are the properties of the toxins when tested against sodium channels expressed in mammalian cells (as opposed to Xenopus oocytes). In any event, the exploration of the syntoxin and contratoxin properties of μ-conotoxins and their derivatives opens a new window through which to view the pharmacology of sodium channels.
This work was supported by National Institutes of Health Grants GM-48677 to G. Bulaj, B. M. Olivera, and D. Yoshikami and R21 NS-055845 to G. Bulaj and D. Yoshikami.
G. Bulaj is a cofounder of NeuroAdjuvants, Inc. B. M. Olivera is a cofounder of Cognetix, Inc.
We thank A. L. Goldin for the NaV1.2 clone, L. Azam for cRNA, and R. J. French and J. R. McArthur for providing the structural coordinates for NaV1.4.
Present address of A. Walewska: Faculty of Chemistry, University of Gdansk, 80-952 Gdansk, Poland.
- Copyright © 2010 The American Physiological Society
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- Andresen and Du Bois, 2009.↵
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- Choudhary et al., 2002.↵
- Choudhary et al., 2003.↵
- Doyle et al., 1998.↵
- Dudley et al., 2000.↵
- Dudley et al., 1995.↵
- Fiedler et al., 2008.↵
- French and Terlau, 2004.↵
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- Heinemann et al., 1992.↵
- Hille, 1975.↵
- Hille, 2001.↵
- Hui et al., 2002.↵
- Khoo et al., 2009.↵
- Li et al., 2001.↵
- Lipkind and Fozzard, 1994.↵
- Lipkind and Fozzard, 2000.↵
- Moczydlowski et al., 1986.↵
- Morris et al., 1998.↵
- Morris et al., 2009.↵
- Mulcahy and Du Bois, 2008.↵
- Nishikawa et al., 2004.↵
- Penzotti et al., 1998.↵
- Penzotti et al., 2001.↵
- Safo et al., 2000.↵
- Santarelli et al., 2007.↵
- Sato et al., 2008.↵
- Schantz et al., 1975.↵
- Terlau et al., 1991.↵
- Terlau and Olivera, 2004.↵
- Tikhonov and Zhorov, 2005.↵
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- Zhang et al., 2009.↵


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If you can't stand the heat Status QuoCD
12,99 €
Product Details
|Hard Rock
|Deluxe Edition, Re-Release
|Media format
|Media Packaging
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Status Quo presents the 2 CD deluxe edition of the three albums: "On The Level", "If You Can't Stand The Heat" and "Whatever You Want" - each in a beautiful digipak (with extended booklet and new liner notes of classic rock authors like Dave Ling as well as rare photos from the band's archive).
"If You Can't Stand The Heat" was released in 1978 and comes with the classic "Accident Prone" and "Again And Again". The tracks were remastered from the original tapes by Andy Pearce and Bob Young. The 12 tracks bonus CD contains unreleased demo versions
Tracklist - If you can't stand the heat
- 1.Again And Again
- 2.I'm Giving Up My Worryin'
- 3.Gonna Teach You To Love Me
- 4.Someone Show Me Home
- 5.Long Legged Linda
- 6.Oh! What A Night
- 7.Accident Prone (Album Version)
- 8.Stones
- 9.Let Me Fly
- 10.Like A Good Girl
- 1.Accident Prone (Single Edit)
- 2.One By One (Early Demo)
- 3.One By One (Demo)
- 4.No Time Left To Cry (Early Demo)
- 5.No Time Left To Cry (Demo)
- 6.Keep Me Guessing (Early Demo)
- 7.Keep Me Guessing (Demo)
- 8.Late Last Night (Demo)
- 9.Late Last Night (Early Demo)
- 10.Invitation (Demo)
- 11.Invitation (Early Demo)
- 12.Again And Again (Demo)
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text:
Beer, Wine, And A Day With The Ski Patrol
Man, what a weekend.
Saturday, we hooked up with some friends to go to the Spring Beer & Wine Festival at the Oregon Convention Center. That rocked. Great booze and a humongous exhibition hall made for an excellent time. Even though there were tons of people there, there was so much space that you didn't feel cramped (like at the Holiday Ale Festival). I didn't take any notes, so I don't have a report for you, but suffice to say there are some really great local stouts out there (especially the Black Bear XX Stout from Alameda, and the one from Roots), and I found another scottish ale I really like (Karlsson). I had some 15-year-old MacTarnahan's scotch as well. Yummy.
Sunday was spent at the Mt. Hood Ski Bowl. I went up there to be a "meat puppet" (as one of the hill apprentices called it) - basically a warm body to ride in the sleds for one of the hill-first folks to do their sled test. Apparently, she'd passed the test on Saturday, so my day all of a sudden got more interesting.
I got paired up with the training director, John, and after running through some opening procedures (radio to RC what equipment was at the top of Multorpor, clear and tighten some rope-lines, and adjust some lift tower pads), actually got to do some one-on-one sled training. I took a familiarization run with a "clipper" sled, learning the ins and outs of making traversing turns and just getting a feel for hauling around an empty (except for the sled pack) sled. After I finished my first run, John had me rig the sled up for transport up the chairlift. I'd helped with this before, on my shadow day, so I pretty much knew how to rig up the bracket. That was kinda trippy actually getting on the lift with it.
The next run was with John in the sled. Holy crap, what a difference. Glad he showed me how to use the drag-chain "brake". I found out on that run just how out-of-shape I am, and how weak my arms are. I had to stop a bunch of times to rest. I was probably gripping the sled handles way too hard, though, because it was my forearms that were just burning. At least I didn't roll the sled and dump him, but I kept having to stop and shake out my arms. I managed to keep pretty decent control of the sled, and I think I even surprised John by actually switching sides when I was sideslipping with the sled.
Now I have to add some different arm stuff to my workouts whenever I get around to actually starting them.
Later, John and I headed up to the Rescue Center at the top of the Upper Bowl, so he could show me around up there and get an understanding of what equipment was up there and stuff like that. I got to check and clear an oxygen bottle, like we've done in OEC class, but I felt like an idiot for not recognizing a suction device. I'd remembered seeing one in the textbook.
Anyway, after that, John took me with him on a couple runs in the Outback area of the Ski Bowl. We did one run on Calamity, then took a hike over to Tom Dick Peak, which is at the western boundary of the ski area. We could see some small slides to the west of the bowl, and just a magnificent view (despite the cloud cover) to the west. We skied from there down the Western Boundary trail, down Black Label, then the Log Road all the way to the base of the bowl. We grabbed some lunch and John showed me around the west side Aid Room.
After lunch, John asked if I was up for some more sled runs, and I said, "sure." We headed up to the top of the upper bowl and were going to take the Skyline trail over to the Multorpor chair to grab a sled, when a call came over the radio (they had me carrying one, too, as well as an avalanche transceiver - I felt almost like a real patroller) about a guy on a snowboard who'd crashed on Skyline. We headed down there, since we were already heading that way. After a quick assessment, we called for a sled. Two other patrollers arrived with the sled and we got the guy off the hill and down to the aid room. I helped out where I was needed, but with 3 patrollers, it wasn't much more than helping line the sled up next to the patient and ski down the hill with his snowboard.
Once we got to the aid room, I helped out in there by taking his BP and pulse, and helped get him ready to head down into town so he could get checked out at an urgent care facility. Just as I was getting ready to go back out on the hill with John, a call came in for another case that would have to be taken care of at the aid room. John figured it would be another good training opportunity if I stayed around the aid room and helped out, so I did. This time, it was just filling out the paperwork and helping the patroller in the aid room put a cardboard splint on the patient's leg. We also ended up carrying her to her sister's vehicle, because we couldn't get a wheelchair up the hill from the aid room (flat tires!).
Anyway, I was pretty beat after all that, and we ended up missing the last lift to get back to the "palace", so we bummed a ride over there with another patroller (who happens to be a transplanted Vermonter like me) in his '73 Land Rover. Cool old vehicle, which he and his dad had restored.
Good day on the mountain, despite how tired I was (and how sore I am now!). Got in some good skiing, and got some early training in on the sled stuff, aid room procedures, and all that.


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Flowers and there meaning to me!
I'm a huge flower lover. I can remember as a little girl going to a botanical garden in Saint Louis, Mo and seeing hundreds if not thousands of different flowers. Everywhere I looked there were different shapes, sizes, and colors … Continue reading →


text:
Mugshot of Reality Winner, who is being held at Lincoln County Jail in Georgia. The prosecutor said investigators also found the names of three Islamic extremists known to federal authorities listed in Winner's notebook.
NSA leaker Reality Winner will plead not guilty to charges she leaked classified information to a reporter, her lawyer told NPR Wednesday.
Victor is accused of "removing classified material from a government facility and mailing it to a news outlet", according to a federal complaint.
Wearing an orange jumpsuit and leg shackles, victor pleaded not guilty in US district court to charges that she illegally retained and transmitted national defense information. "I don't think she's seeing a light at the end of the tunnel", Billie Winner said.
Reality Winner was caught because she was stupid, not because of anything the reporter (s) did or didn't do.
If convicted, victor faces a maximum sentence of 10 years in prison and/or a fine of up to $250,000, according to court documents.
Prosecutors plan to ask a judge to deny bond for 25-year-old Reality Winner, according to court records filed in the case.
Victor reportedly told federal agents she mailed the classified document to the undivulged media outlet, according to court documents.
She started learning Arabic on her own before she finished high school, a language she loved, said her mother, Billie Winner-Davis.
USA protectionism poses sizable risk to Mexico growth
The OECD has raised its 2017 GDP projection for Poland to 3.6 percent from 3.2 percent in a November forecast. Global growth remains below long-term averages, however, and wages are not yet growing as much as hoped.
Gary Davis, Winner's stepfather, said she turned down a full college scholarship to join the Air Force. She's never run away from anything in her life.
In the process, the intelligence agency noticed that the reports "appeared to be folded and/or creased, suggesting they had been printed and hand-carried out of a secured space", according to the official legal complaint.
Winner also has a Twitter account under the alias Sara Winners - although she had a visible profile photo of herself - where she was repeatedly critical of President Trump.
After authorities determined the model and serial numbers of the printer that produced the secret documents, as well as the date and time of the printing, they were able to arrest victor.
Victor also told her mother she feared for her life and the life of her pets, and asked her mother to "play up that angle", Solari alleged.
The judge said he was anxious about what happened to the portable hard drive prosecutors mentioned, as well as references victor had made in her notebook about the Middle East.
Winner posted under a pseudonym, Sara Winners, but didn't seem concerned with concealing her identity, using a photo of herself as a profile picture and posting a selfie in February. "She's going to be made an example of", Winner's mother said. "She serves her community; she served her country".
Prosecutors said a phone call to her sister expressed Winner's confidence in how to play the court during her bond hearing.
The Department of Justice has said victor admitted to the accusations against her, but Nichols, told the Associated Press "if there is a confession, the government has not shown it to me". They say they were able to narrow down the source using the unique "microdots" in the printout, which identify the source printer, as well as the fact that victor had been in email contact with a news outlet, presumed to be The Intercept.


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*Seeing is Believing is a novelette. This is not a standalone story. It must be read with the series to be understood.*
About the Book
The life of a Seer isn't for the faint of heart. I should know. Mine has been one of upheaval for the past few years, what with the downfall of the lab, the recent exile from the Council, and a future that is suddenly becoming painfully clear.
With Izzy missing, and the darkness still looming, I know what must be done. I have to go to the Order, find a way to bring Izzy back, and get to the bottom of what is really happening with this darkness business.
I'm just not sure how to do that when Ian is haunted by his past, Revenants are out to make me one of their own, and the dreaming keeps trying to reject me.
Look Inside


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Demonstrators may be asked to leave St. Paul's in London
St. Paul's Cathedral raises doubts over protesters
Local churches in Joplin help to rebuild houses with "Extreme Makeover: Home Edition"
TV makeover show to build 7 homes in Joplin
Afghanistan lack in religious liberty
Watchdog group laments lack of religious liberty in Afghanistan
< Euro In Trouble
Sep 28, 2012 06:18
Category: General News
Category: General News
Clinton To Campaign For Obama On Debate Day
WASHINGTON (AP) — Former President Bill Clinton is going back on the campaign trail stumping for President Barack Obama. He'll appear in New Hampshire Wednesday, the same day Obama and Republican rival Mitt Romney meet in their first debate. Romney has a rally in Pennsylvania today along with some fund-raising. Obama also plans fundraising today.


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Have you ever given a thought to the idea of "what keeps us going forward in Life"? Human Being are full of desires and these desires are what keeps any human being moving forward. Don't you agree with me? Let us look at examples. Most people have these varying dreams/desires for eg: they want to be… Continue reading Ever wondered, why is it so important to have something to look forward to?
Tag: Life
The Excitement and Adventure of going to my Best Friend's Wedding
I never thought that I would be living in another corner of the world when my best friend in India would decide to get married with the guy she fell in love with. Yes, she found the guy who makes her believe in love, fairy tales, magic and most of all, he makes her the… Continue reading The Excitement and Adventure of going to my Best Friend's Wedding


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Outfit Details:
sweater - Joe Fresh, jeans - Gap, necklace - H&M, wedges - Gucci
Ever have a terrible TERRIBLE day at work? I'm having one of those. And it's only noon.
It's got to get better right?
Sorry for the bad day :(
Love the outfit, though! Especially the shoes!
You can take a deep breath and say "I'm rocking the nautical look, I'm rocking the nautical look, I'm ROCKING the nautical look" and hopefully you'll feel better!
Thanks for the kind words Becca and igetdressed. Things are better this week (albeit still a bit crazy). Still swamped at work, but at least I met my last deadline!


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On my recent trip north to our summer cabin high atop the mountains of Piney creek, North Carolina, we took a southward detour to visit my long time pal and fellow humor illustrator Fian Arroyo in his new home in Asheville.
We then made a 3 hour drive to Winsten-Salem for a first time face-to-face meeting with another fellow illustrator I met on twitter, the talented Bob Ostrom.
This is a candid interview with the Bob Ostrom as he shares some great information on his career as a cartoonist, how he got started drawing children's books, freelancing, marketing and chicks on motorcycles. Enjoy.